LOCUS       DANLXF010000001        34309 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.001, whole genome shotgun sequence.
ACCESSION   DANLXF010000001 DANLXF010000000
VERSION     DANLXF010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 34309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34309
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.001"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            23..241
                     /locus_tag="PXD66_000001"
     CDS             23..241
                     /locus_tag="PXD66_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL6318345.1"
                     /translation="MARNKRVRDMSNFEIRGELMKIGKSLSQLGIENGLAKTTVRNAL
                     DKPYPKGEQIIAKAIGKTPQEIWPSRYE"
     gene            251..2221
                     /locus_tag="PXD66_000002"
     CDS             251..2221
                     /locus_tag="PXD66_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HDL6318346.1"
                     /translation="MKEWFLAKEIAGLSGVPELPNSVSRLATKEGWQKRQIQGVRGVT
                     YEYHLTSLPIETQQQLRLNAALAVIPQAAELQPKRDDPALIARLNNATDKGRDKAKGK
                     AEACMQLQAFLDQGFSYTQAEAGAATAKNVSQGSLKNWYYKVKGHPVHLWQAILISES
                     GRSKKPQLKAKITEEAWDCFLADYLRPEKPDLRASYRRTQAIAKQYGWQIASLQTFQR
                     RVLAEVPYEVILLKREGANAVAKLVPALQRTVKDILAGEWINGDGYQHNVFVKWHTGE
                     IVRPKTWFWQDVRTRKILGYRTSISENTDSIRHALMDVIFNVGIPKTLTLDNTRAAAN
                     KAMTGGIENRYRFKHTELDPKGIMPILGIDVHFTSILYGEGHGQAKPIERAFGRGGIG
                     EKIDKRPELSGFYTGRNAQETPDNYNGGKDGVDYNTFLKAIAAGIEEYNSQTERHTEM
                     CRGELSFNQVWERDYHPSNVRQASPEQLRLLFLQAETVSIKRNGSFTLKAAGKLYGLT
                     NVYWAESLIGITDKKVVARFDPDDLHGNVYVYNLEGQFLAEAVCREAKGFGDTSASRE
                     QGRLYQKVVKSAKAQAEALELLEAHELASLAPQVDVPEPIEKKVKEVLVKEDFIVDFN
                     TVRKATVVEETEEISIFDEVLLKQEKQLRIVE"
     gene            complement(2401..2889)
                     /locus_tag="PXD66_000003"
     CDS             complement(2401..2889)
                     /locus_tag="PXD66_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318347.1"
                     /translation="MLFRSLTDDEIHQIALDSARATFSGFKLKRANAIVMKNCITLFK
                     LSTINFCLAEYHSSFHHLTSIDDLDGIMPTVKKVAQMLSLQESKALIWLLYLKWLFVM
                     RQRSLQVLLENSADLIQGEVIAQPLPTFVKAVLAFQLETYHGLPEWIPEFDSAEFWQS
                     LS"
     gene            2921..3862
                     /locus_tag="PXD66_000004"
     CDS             2921..3862
                     /locus_tag="PXD66_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL6318348.1"
                     /translation="MTLIDQIKQHIAESGSSQGKVAKEAGINAGALSAYLNENYKGNN
                     EELEAKLIAYLERIEAKKREFVEAPSFIETKTANQIFGSLRFAQNTGVLAIIHGASGV
                     GKTQAAREYRKRYANVWLVTASPSRSSLSEVLYEIALELGMNDAPRRKGTLARLIVRK
                     MKGTAGLLIIDEADHLPYEALEELRLLQEEVVNESDIGAGVGLVLIGNDKVYTRMKGG
                     INPAHEYGRLWSRSAKRTSIQKTKKEDTQAVAKAWGLSENQEALKVMQAITETGGGLR
                     ILTHTLRLAAMVAKDRMIPLTADLIQLARKDLLSTGD"
     gene            3865..4179
                     /locus_tag="PXD66_000005"
     CDS             3865..4179
                     /locus_tag="PXD66_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318349.1"
                     /translation="MRTPKPKHSFNRTNQAACRFLKQTQKAIIRLNSLGFTVLNIDFT
                     RIKPRIEVEIGNNKHIAQGLIYEGKAYRYSFGKSEDLGRWEGYYTMLEGIRVWWRQAA
                     IH"
     gene            4191..4427
                     /locus_tag="PXD66_000006"
     CDS             4191..4427
                     /locus_tag="PXD66_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318350.1"
                     /translation="MKTLALLTALLGLVGCDLVQSKQAKNYIGNNLTEICIDNVVYLI
                     YSGDKKGGITPKVNKDFYPYTCTNKEERQNGKTR"
     gene            4411..4704
                     /locus_tag="PXD66_000007"
     CDS             4411..4704
                     /locus_tag="PXD66_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318351.1"
                     /translation="MAKPDETPITKAECQSQLAELGVQYKKLPMAITRHICNATTNIH
                     GKVIKVSVVERVGYGVQITAQGNEKSCLVTYEAMLGMAEAMGLFDEVKEQNND"
     gene            4826..5044
                     /locus_tag="PXD66_000008"
     CDS             4826..5044
                     /locus_tag="PXD66_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HDL6318352.1"
                     /translation="MNDELAAISASYAQHNRYQHYAEKAAEAERKGNYKEAQDHWEVA
                     KLSAKKTANRDWAEQRAEFCKRMHNKPF"
     gene            5054..5236
                     /locus_tag="PXD66_000009"
     CDS             5054..5236
                     /locus_tag="PXD66_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318353.1"
                     /translation="MTETVKAQLNSQLNEAIIQLIQAQKYLNQDDAIRSGVYIGTVQD
                     LLPKVHLKLLTANRKH"
     gene            5253..5642
                     /locus_tag="PXD66_000010"
     CDS             5253..5642
                     /locus_tag="PXD66_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318354.1"
                     /translation="MAITEEQWKEIKQKLDSIIGRVKFRYKEHLLTVDVVQIKRSLKL
                     AVYVDGEIDGAWTKEGHEIRPYLEEVWYRKERPFFNAKEKKEYRGLMSKKKLNEKIVT
                     YSPMFPSPTALTRQYKKLDGLELIEVI"
     gene            5691..5978
                     /locus_tag="PXD66_000011"
     CDS             5691..5978
                     /locus_tag="PXD66_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318355.1"
                     /translation="MNKNEFFKKYHLEGSLAILDLDANDDQSQFCYQALLEYRELPLD
                     TFLAEYSDDEDMTESEFIELMLDELEWIIRRAKNAKRFVKKRQKTMREEIM"
     gene            6097..6642
                     /locus_tag="PXD66_000012"
     CDS             6097..6642
                     /locus_tag="PXD66_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1018 domain-containing protein"
                     /protein_id="HDL6318356.1"
                     /translation="MTDKAKLIQLIHIAKSQLAMDDLSYREMLKRLTNKTSSTKCTVV
                     ELHKVLHELQTKGAKVKYFAKRSKKPTAYSPATGETAVKSQIAHKIRAVWITMGKHGF
                     LNDPSEKALNAYMRKVINNKTRPTILLNVSALEAGDASYMLEILKKWHKRVMIKALSK
                     SCTVDPKISYDELVEWYNEVM"
     gene            6629..7162
                     /locus_tag="PXD66_000013"
     CDS             6629..7162
                     /locus_tag="PXD66_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318357.1"
                     /translation="MKLCRCPICHSDIHLEALVEDEAGRELLGKISQLTHGVAQPMVA
                     YLGLFKPQKSNLNNSRALKILNDVLALYPCSLLLAQALSETVVSIRKKRQQAMENGQK
                     IEPLPNHNYLKSVYETQKVHFAVVRTGKTERETMKAELEKTRNAIAYVQRFVDLGREE
                     VVKNSPEYQIWLAHKPK"
     gene            7327..7686
                     /locus_tag="PXD66_000014"
     CDS             7327..7686
                     /locus_tag="PXD66_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL6318358.1"
                     /translation="MELVDIFEEKAPEVLTDLAKHIEVALEEKCQFSPEKAKQIGIDI
                     AQQMAQNWGGEVIYIPRNLLMALNERDMKIYEEFNGNNHRELARKYNVSMQWVYKIVK
                     KVHKDEVARRQMSMFEM"
     gene            7822..8331
                     /locus_tag="PXD66_000015"
     CDS             7822..8331
                     /locus_tag="PXD66_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HDL6318359.1"
                     /translation="MSQNLTFRQIFDRLIGHEGGYVNHPQDPGGETHWGITKRTAVAN
                     GYMYAMKNMTREQAYQIYEKAFWQRYRCAELKPAVAYQFFDAVVNHGFGNASRMLQRA
                     VGVADDGIIGNITLQAINAKSESDLLMLFNAERLKFYTKLRNFPTFGKGWVNRVAGNL
                     EYAAQDNEV"
     gene            8334..8603
                     /locus_tag="PXD66_000016"
     CDS             8334..8603
                     /locus_tag="PXD66_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2644 domain-containing protein"
                     /protein_id="HDL6318360.1"
                     /translation="MAKLFKELLSTNGTLSTTSTVQFFGTLCVFGLMVYAVITQQPYA
                     ESLLNNVLIYLFGATTAKGVVTSYQAKIKGGIHKQQSTFKEEEGC"
     gene            8597..8944
                     /locus_tag="PXD66_000017"
     CDS             8597..8944
                     /locus_tag="PXD66_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2681 domain-containing protein"
                     /protein_id="HDL6318361.1"
                     /translation="MLMQQILGVIGVIAAILSYALFKSWQLKRERKKNAILQAEKQQQ
                     AVEIEQKKAEVKYVYISKKNNEKVTRSSASDIDDKLQQHGYFRDNNRLHGVQSDLSEP
                     CGYAGNETSDSCS"
     gene            8971..9123
                     /locus_tag="PXD66_000018"
     CDS             8971..9123
                     /locus_tag="PXD66_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318362.1"
                     /translation="MKYIKKLRKRWQIWRFLKNHPEVAQRRDLLAIAIKQGVENPVGR
                     ARRREP"
     gene            9123..9458
                     /locus_tag="PXD66_000019"
     CDS             9123..9458
                     /locus_tag="PXD66_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2730 domain-containing protein"
                     /protein_id="HDL6318363.1"
                     /translation="MDLFTVFWESIRANFGFISALLTIVVTAFWLKLDSKYAKKNDLG
                     ELLEIAKSHDNRLTTLETKVENLPSAVDVEKLKTLVTDVKGDTKATSKQVDSISHQLG
                     LLLEAKLKE"
     gene            9460..9756
                     /locus_tag="PXD66_000020"
     CDS             9460..9756
                     /locus_tag="PXD66_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318364.1"
                     /translation="MSLRDLLTQDQRLVILRSLAEAGYDANESIISDCLDLYGHDISR
                     DLVRTHLSWLEEQGLLTLERLRDGYMVASITQRGLDVAQGRVVVSGVKRPRPKI"
     gene            9779..10351
                     /locus_tag="PXD66_000021"
     CDS             9779..10351
                     /locus_tag="PXD66_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3486 family protein"
                     /protein_id="HDL6318365.1"
                     /translation="MSEKNTRGRASKVDLLPPNIKTQLAMMLRDKQYSQSQILEEIND
                     LIRDCGLPDEMLLSKTGLNRYASRMERLGAKIRNAREVAEIWTKQFGEMPQTDIGKTL
                     MEMVKQIAFETSLKLGEQEGGIEPKQLALLSSAIQRLEQAESLSFKREQAIRKEVAQQ
                     AAETAEKVVVQAGLSAETVRTIKEQILGIA"
     gene            10351..11907
                     /locus_tag="PXD66_000022"
     CDS             10351..11907
                     /locus_tag="PXD66_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HDL6318366.1"
                     /translation="MALLNNRPLNELAPECRSFLESIHAFNPNELLLGYQKRWIADDS
                     QLKIAEKTRRCGLTWAEAADNALIASTRKSDGGSDVFYIGSNKEMAREYIDAVAMWAK
                     AFNRVAGEIQEEIFEDEDKDILTYVIYFSSGFKVKALSSNPKNLRGMQGVVVIDEAAF
                     HEYLAEVLKAALALTMWGAKVRIISTHNGSENLFNELITDSRAGRKRYSVHTITIEDA
                     CRDGLYQRICQVVTKKTWSPEAEKEWIENLLNDTASEEDALEEYFCVPKFGSGLWLSR
                     ALIERQMSEATPVIRFTAKDGFSLVAEPTRYAEIAEWCELELEPILATLNPDLLHFMG
                     EDFARSGDMTSLVVLAQQQNLVRKVAFIVELGNMPYKQQEQIVMFMLKRLPHFAGGAF
                     DARGNGGYLAEAARDEFSTLIDCVQLSEKWYREHTAPFKAALEDGELEAIPKDADILA
                     DLRSFQVVKGVPRIPDKRTKSADGKSKRHGDTAISLLLAHYASRQLVQMPVEPVSRRP
                     RHSQQLTEGY"
     gene            12026..13480
                     /locus_tag="PXD66_000023"
     CDS             12026..13480
                     /locus_tag="PXD66_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HDL6318367.1"
                     /translation="MAKKNNLISEIATRARSIDYWAFGYYLPNPDPILKRMGKDISVY
                     RELLSDGQVRSGVRRRKAAIKGLEWRITTTNNEKVDEQIYQIFNRLPMNNLITEMLNA
                     ALYGYHISEVIWAKEGELFVPTEIIGKKPEWFMFDDENLLRFRTKENWADGELLPDDK
                     FLLTTQEATQDNPYGLGDLSLCFWAATFKKGGFKYWLEFTEKYGSPWLIGKHPRQAHN
                     TDKERLADALEGMISSAIAVIPEDSSVEIKDAAGKSASTDAFEKFLNFCKAEINIALL
                     GQNQTTEQESNRASAQVGLEVMEDIRSDDQAMIEATFNTLLQWIVKYNFNVEQLPKFE
                     FYEQEAINTDQVERDSKLHAMGVRFSKAYFEREYGFEAGDIEVQAVEKAEKSANTTEF
                     SEGQRRTQVPMPVPILEQLEHEGEAMVDDWLQGVRDKLAQADSLEDFQSQLDSLIPEL
                     SFAEYGELLALGSTVAELAGRQSVNDEIKAKRNA"
     gene            13473..14750
                     /locus_tag="PXD66_000024"
     CDS             13473..14750
                     /locus_tag="PXD66_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HDL6318368.1"
                     /translation="MRKFTFKQQAQYFEKKLNLRTNSYLDILGEEHDYFFVVAGANRN
                     EVLSEFRKAVDEAIHNGETLENFRKRFDEVVAKTGWEYNGGRNWRTRIIYDTNVYGAY
                     NRGRLQQHLELAEDMPYWEYQHNDNAHPRPQHVAWDGLVLRYDDPFWKYHYPIKAYGC
                     HCTVQALDDFDLKHDGKTVSPPPEIEFEQKTVGVRTGNSRVVTVPKGVDVGFTPHNFD
                     NLKAGRAANVDKLLMQKMVTADPIFASKAVSDLLAKEPVAKLHNQAFKNWVNDVANAK
                     GSALSQVENMQLLGVLPVAVIERLQQINLAPQSAIIAITKGDMVHALRELKQNTGIAV
                     PLEFWEKLPAKLQYPNAILLEKQQAETTLLFIYDVAQTKIAVKVNYDATLQDRHGAKG
                     RQKVLLNRAVTGSLFQDTTGLKSSKYEVLLGEM"
     gene            complement(14952..15296)
                     /locus_tag="PXD66_000025"
     CDS             complement(14952..15296)
                     /locus_tag="PXD66_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318369.1"
                     /translation="MMVNAEQAIELVYDLLLSRQWLVTKAEKLPLDPLSEKEAVMFLY
                     TLDQQTEASWLQLTPEQRATANGLIMDFIAKCLTSTKQWLVSDNIVPELQAIEIIKHE
                     IFLSHNSLVMPN"
     gene            15360..15824
                     /locus_tag="PXD66_000026"
     CDS             15360..15824
                     /locus_tag="PXD66_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HDL6318370.1"
                     /translation="MIKITLNNTQAVKSLQQIANQLEQPRRLYGILGETLKKIHTARF
                     KAEQDPDGNNWKPLASSTLALKRKRGKSTKILRQDGYLADKTAYNVTSDNVEFGSQEV
                     YARLHQFGGKAGRGKKVTVPARPWLGVGDKDEQLLLRKAEWHLGQIIGRMGK"
     gene            16059..17174
                     /locus_tag="PXD66_000027"
     CDS             16059..17174
                     /locus_tag="PXD66_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDL6318371.1"
                     /translation="MTQIEIFKAGKRYDANGTLIDITPEMLQQTVAAYNPEFHEAPLV
                     IGHPKSNNPAWGWVKALSLEGDVLKAEVDQLDAEFSEMVASGKFKKVSAAFYLPDSPN
                     NPHKGVLSLRHVGFLGAMPPAVKGLKQVEFAEDDDFVVFSEWGVASLFSRFRDWLVSE
                     FGLEKADKALPPHEIDWLKEEAMRSQISKQVHAEQATPEPIFNEPAQPEGEPEMSAEE
                     KEELERLKAENEQLKAEKAQAEAEKMEAELEAEKAGNAEFCEGLVKQGKLAPVAKAAF
                     VQALNNLSVLKAGREPEFNEGEDVVSQFKSAMSQSPQIIQFGEYATADKANPQEAEAV
                     EYAEGTDPVSIEADQKVRAYMKAHNVGYAAAFNAIYR"
     gene            17205..18131
                     /locus_tag="PXD66_000028"
     CDS             17205..18131
                     /locus_tag="PXD66_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007524268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HDL6318372.1"
                     /translation="MTTHNLQKLRVQDPVLTELAQGYHNNELVAETLMPTVEIEKEAG
                     KIPTFGRLAFRLPSTVRNLRGSSNRLDPEDIGAIDVALEEHDVEYAIDYREENEAIFS
                     LRQFALNTTQDVIALGREKAVATLALDESRYETDNKVTLSGTSQFSHKDADVFKVFDT
                     AIRAVKRAIGRKPNVCVISGDVWAALKEHPAVVEKLKYSQVAIVTPEVFAKLIGIETV
                     KIGEAVYEESGQLKDIWSKDIVVAYVAPRSTGGKGTVYEPSYGYTVRRQKGLFVDTYK
                     ESGGKIEVVRTTDIHKPHLVGAAAGYLIKAAV"
     gene            18200..18481
                     /locus_tag="PXD66_000029"
     CDS             18200..18481
                     /locus_tag="PXD66_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318373.1"
                     /translation="MAKFIVLHTPILHDGKRYEVGAEIELTAEQAENNAINLKAVEAV
                     PKPPKNASKPEDEPKPEGEPKPEDAVTEDDVKLAEAKIAEAEQPKGNKK"
     gene            18481..18915
                     /locus_tag="PXD66_000030"
     CDS             18481..18915
                     /locus_tag="PXD66_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 domain-containing protein"
                     /protein_id="HDL6318374.1"
                     /translation="MYVQIDELLTAFSRKIVVQLSNDEPTATEPDLKMLETAVKVANE
                     RIDAALRSRYTLPLADVPTLINQHALTLARYWLYARRPETKMPDTVKETYQQAVKELE
                     QIATGKLHLGIAESSQILEDDVLADNSEYEVRANARIDTSRY"
     gene            18921..19418
                     /locus_tag="PXD66_000031"
     CDS             18921..19418
                     /locus_tag="PXD66_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp37 family protein"
                     /protein_id="HDL6318375.1"
                     /translation="MSATQPILEDICQHLRQALPEWEVELFPNDPSTYHLSHINGAVL
                     ISYLASKFEKPRTTQAVLQVRHVQIALTVLTRDLHNDVGALDLLDKLRLLVVGFKPQN
                     CGECWLIDEFFNGSSEETGLWQYQLILQTETQQVQHQAVENLPKFVTAHLRRQDQPGS
                     PGLKT"
     gene            19503..20888
                     /locus_tag="PXD66_000032"
     CDS             19503..20888
                     /locus_tag="PXD66_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HDL6318376.1"
                     /translation="MTFHHGSETERVNGGSVPVSVVDSAIIGIVGTAPIGAVNELTVC
                     LTKKDFAKFGTILNQGFTLPDAFDILARYAAGKVYVVNVLDPSRHRTTVTDEVLTLDR
                     NTLTAQTAKTGIIAVTRVKDGANVLNADRYSVDLQTGVVTFVSAPSAPKISYIYADPS
                     KVTEEDIKGGVDSLTGKRKGFELLRDGFNLYGADAKILICPEFDKTASCAAALATLAD
                     QLHAKAYIQLPKGTSLSKAIQGRGPAGTINASASNENVRHFFPYALGSSNTLESLATH
                     AAGLRMLIDVEKGYWFSTSNKELKGVIGLEIPLTARVDDKQSETNLLNAVGITTIFNS
                     FGTGFRLWGNRSSNFPTVTHISNFEVASRTGDIIDESIRQAELQFIDLPIDDALLDSF
                     LETIDTFLRTQKSLVGYSVSLDYEYDLVDAFSRGQVPIVYEYTPKIPGERISNKSVMT
                     RKYLANLVSQR"
     gene            20899..21414
                     /locus_tag="PXD66_000033"
     CDS             20899..21414
                     /locus_tag="PXD66_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HDL6318377.1"
                     /translation="MSIAINQIVNGNVYINGNSQMGRVNEVKIPDIEFEFIEHKGLGM
                     HGVIKLPAGTNAIEAEITWDSFYPEVRAVMLNPYKYTQLQIRSNLQVFNSQGLAAEEP
                     MVTIMNVSAGKIGGTGHKNKENAEFADTLNVHSIKQTVAGRELLFIDVLANIYRVNGV
                     DVLQKFRTNIG"
     gene            21510..21824
                     /locus_tag="PXD66_000034"
     CDS             21510..21824
                     /locus_tag="PXD66_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HDL6318378.1"
                     /translation="MANAAQQVLTNLRVQTTYKLQYPIALPDGRVIETLDVRRPKGKD
                     FRLLDEKGFDPEKDGVKILRFYIQQLTTLVPEDIDELDAADIKGLNNLLEDMLIEGKS
                     DK"
     gene            21821..21973
                     /locus_tag="PXD66_000035"
     CDS             21821..21973
                     /locus_tag="PXD66_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HDL6318379.1"
                     /translation="MSELDTVYADLAWWFKFSPSELLELDLVDIPKWVEQMNRQIKAG
                     YGQILR"
     gene            complement(21952..22254)
                     /locus_tag="PXD66_000036"
     CDS             complement(21952..22254)
                     /locus_tag="PXD66_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318380.1"
                     /translation="MLTKLNNRITEWAGFEACVTRTECTAKWLLAVALVGWSIFYWLD
                     AIQSIQSLSEGVSFVLIYGLILTILAWVGVYLAWGVAILLTLATEPFIWLYRKICP"
     gene            22302..24959
                     /locus_tag="PXD66_000037"
     CDS             22302..24959
                     /locus_tag="PXD66_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL6318381.1"
                     /translation="MANNLAIGMVIGASLSSGFHGAFSGAKKKLEQLEQAVAKNQQSH
                     SKLGSELTALRSKQATLYAEMSRASSKGGAGLALMQQEYKKIGKEIANISREQRKWST
                     ELEKSIAKQTKLRNAMERHTQAKENRDELKGKIIGTVASAAMGVGVMKTYMEQEEAAN
                     NLKISMLKADGSFGKFEEIGKIADTLGTDLPGTKKDFYKLAMALKKQGVSDDVLTGGA
                     LKTSAELNVLLDMDQESGGEFLAKFMESHRLNENELPQAADYLQRAMFAGGLSKEQMY
                     ESMKYYAPKLNSMKLTGAENTEKVLAIEAMAGQQGLEGSTFGTGLNMMLSRMNKGPEM
                     LKAAAKGMKAEAREMMEAVGVEFNFWDKNGSFKGIDGMLAEMTKFEKIRKKFGDKGVG
                     LVAEELFGIEGGRLADILAMKGQKGLDEMLSKMREQASLQDRINLKTNTLGAALESLG
                     GAWETFVGDIGSVFAEDIKGFSKGLQSAVEWLVPFVSKHKEAIKFVGGLVAGLVILRG
                     VLQAGSFVIQTVTMAMNALKIAAMTNPIVLVIGAIAAGAYLIYSHWDSIAPWFANLWQ
                     NVTAYFNQFWTWVKGVWSGVTDWVSSVWNGVATFFGQLWTTITSLFSVAWTNIQTIWG
                     GATAWFSGLWDGIKSAAFGMFDSISQKVSSVVASIKSFLGFGDDVDKMGAKIGNVATQ
                     VISKEHSGQLSKTADMAANTGFSSGGFTGQGGKYTPAGIVHKGEYVMTKEATSRLGVG
                     MLDSLNYGGAMGFTQPVLQPILGKQKGFFGSLWDDVKFGANFVGNLLGLNQGSLKTPD
                     FNPNATNASGQNSSIFSNYEPLNRNAVTQQESSQAPQIVVNFNPTINVNGNATQEAQS
                     QIMQQLQMSAYEFEKLLNRVLDQRSRRAY"
     gene            24969..25892
                     /locus_tag="PXD66_000038"
     CDS             24969..25892
                     /locus_tag="PXD66_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6318382.1"
                     /translation="MYFMLGNIAFEPVNLTEFSEQHTAEFAEHAVLKGKPRLQAMGEK
                     LNELNFSLRLHHTIGGVESRYQALLEAKAKQAALALIWGSRYKGDYVIVDISSTTLFT
                     DGRGNALAREMQISLKEFVGDNDGGVLGEALNFGGGSLLGSILPSGAVTTLSQIKTAV
                     SRGVELYNSGKRLVDEVQNTVAIIRQLKNDPATALAYLPAALANLDGALGHFGNLTGS
                     SATLGGLQEKLPAAVVFSEEIGEIYQSLQTMRSSLGNASANSWDNWFTPAESALTEVT
                     DSFDHLAKPVAKMTAWIVLRTDEEAENDTVA"
     gene            25876..26103
                     /locus_tag="PXD66_000039"
     CDS             25876..26103
                     /locus_tag="PXD66_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HDL6318383.1"
                     /translation="MTQLLKHIVKQGERWDNLSYQYYGNAFEYGRIIDANPHIGFCEV
                     LPIGVTIYIPVLNVKPTNNENMPPWLRGRDA"
     gene            26096..27160
                     /locus_tag="PXD66_000040"
     CDS             26096..27160
                     /locus_tag="PXD66_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318384.1"
                     /translation="MRNVPKPDFSLFYEKTNITAEIEPHLVQLTYIDHLEGQSDELTV
                     EFEDIQGKWIRQWFPTQGDKLKAAIGYQGEFLTDIGEFEIDEVEYSYKPSTINLKALS
                     TGISKANRTLKPKAYENTTLAQVVAKVADNLKLKVVGKIKAIPIKRITQYQERDVEFL
                     ARLAREYHHSFKIVGNQLVFTDKSELGQSEPVLMLEERDTISLRLRDRIKDTAKAVEI
                     SGYDTSGKKVVKKRKKAKALRPNIKQAKSASEDTLKIITRGESQEQIDARGEAALATQ
                     NEDQAAGDIELIGNPKLVAGATILLRNFGVFSGKYLIKSSRHTISRSGGYTTHIEVRM
                     LEFIADDLITLGMEAGNANA"
     gene            27147..27683
                     /locus_tag="PXD66_000041"
     CDS             27147..27683
                     /locus_tag="PXD66_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HDL6318385.1"
                     /translation="MQTHNFGATYQEGIVSAVDPKTHKVRCKIPALEDLETAWLSFLT
                     PNAGGNQFYCLPDVGELVAMLLDAQGEGGCVFGAIYNEQDPTPVADSDMWVKKFSNGT
                     EISHNRKTGDVVVKTNGHLTATAQGGATINANTVVNGTLHATGKITSDEEVSAPVVKQ
                     GTVELGTHKHGGSPEPNK"
     gene            27738..28100
                     /locus_tag="PXD66_000042"
     CDS             27738..28100
                     /locus_tag="PXD66_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HDL6318386.1"
                     /translation="MNRQTSIQLTHWQLAPSTDTVEVVQGIDDIHLCIVNILSTQKGT
                     DILRPEFGSDHFRYIDYPEDVAIPNFVREITTALQKWEKRIEVEEVLVDGSAPHFIFT
                     VMWHLTDDVYREIYRTTV"
     gene            28110..29213
                     /locus_tag="PXD66_000043"
     CDS             28110..29213
                     /locus_tag="PXD66_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDL6318387.1"
                     /translation="MTEAIKIIDDDVKTVLAEAIADYEKRTGKILQPAHIERSIIQTY
                     AYREQLLRKGINHAFLQTFPQFATGIALDLCGETFGCYRLRDKPARTLLRFSVSGEHT
                     AIVIPKGTQVAINDELYFSTLNDDVITPLITYVEIDAECNQVGVIGNGWESGRVSKLK
                     TALATNYKVTVENIDITSGGLMQEEDEPYRKRILAAPEAFSNCGSIAAYDYHVRSVSQ
                     DIADVNISTPRGGLVRIAVLTQKGLPDTRLLDDIKKHVSAERLRPLCDMVEVIKPTER
                     RYQITARLILLDGYREDLVKAKAREALQLYLSDKTKKLGLDIVPSAIISALRVEGVYD
                     VNLIQPVKTIINETEWANCTAISVEVEEERRDG"
     gene            29206..29772
                     /locus_tag="PXD66_000044"
     CDS             29206..29772
                     /locus_tag="PXD66_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6318388.1"
                     /translation="MASLHYADIIVRDPKYKALADLSKRLNLLDTSQIMTTLVDLLGD
                     ECIPLLAEKWSVTGYDGLLVADSENSKRALIKRAIELHQLKGTPWSIREVLRRLGFGE
                     VEIDEGLKNRLYESTYVSGIPENERWAHYAIRLSNPITNEQARNIRKVLRNFAPARCS
                     LAVLDYKADPIRYNNKATYNGTYNHGSS"
     gene            29782..32061
                     /locus_tag="PXD66_000045"
     CDS             29782..32061
                     /locus_tag="PXD66_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HDL6318389.1"
                     /translation="MAGVQETAQWENHVYRIEENDPVHGGENGVTNKPIKHLANRTLY
                     LRRLLTEAGQRINPKKITATSKNSNDLTGHTHEIDHASLTQKGIVQLNASIQSTSTTE
                     SATPSAVKAAYDKAATAEQKATLANEKVEAIKVGGRNLLIQSNTPHNGNAYSVRYALA
                     EAPKVGEEIVVTLWGELGSQRTGIGVYNSQGATELAKLVKIRDGVYQGKGIWRKPMSE
                     GRERTPNDTHLNVYFYPKTATDNNRIERIQLERGSIGTDWTPNEADLKAEISAIRTKL
                     IPVRGGNVATTLNAAKRSDWFREMQIPYQSGWGYYTQAFHNSGTQHNYSNLPLTGRYY
                     FIAELYHNSGYSHLRITYPSLKRTFESAINLNNEDLILDWREVVFLKDGLYQGTFKTT
                     GTLESAYRLRAARDDERYQPYLQMVDMGYDLANPPVGKNKIIGSVDYYVKNGTSESSK
                     ATMMAAAMPDKNVFWEVALWNAAQQRNVLFKGYSKTNNLSIGKTTDNERDRVQVNGTI
                     QATAPADNANNDQVPTTAWVRKMAKILADGKVSKAGDTMTGHLFIKNGEYSAVHTYNT
                     SGWFAKWEAAPLSDSHFAAIVYANDRETVINRVLIPKKNGTVALINDIADTVRAAVSR
                     SDKSRSRTFPLQTGKKYTTTGSVTIYPDGRIVQIFHLKNIKAAWFDYEASAVGDRHRV
                     VNIPLWTAMPNKIFDVDVKTVRPSNNASTYYIEAAEWLSAWQIYGNDTNKSSVNINLS
                     RFRGGKDEDMDLYVTVEGY"
     gene            32062..32664
                     /locus_tag="PXD66_000046"
     CDS             32062..32664
                     /locus_tag="PXD66_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318390.1"
                     /translation="MAYFVNVIDEQGNYELIDDEFIGLYPDLDFNTLPKLTDKQYQTY
                     LAKANGKERFVNGEFVYEQIKVDMQAVISAEKSAKLAEINQKAQAFINDLAKYNETPK
                     FERDTWLEQAKEAKAWVADPTVQTPTLELIAQMRGIPLDTLRQKAYEKAMAYQTVAAI
                     VAGQRQGYEDRLEQAETLEQIQAIEPMYQLPQGANNEQTD"
     gene            32648..32923
                     /locus_tag="PXD66_000047"
     CDS             32648..32923
                     /locus_tag="PXD66_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318391.1"
                     /translation="MSKLTKLKSWSYHVLIAIDQLFNALTGGAADETFSSRCYRGAVL
                     AENPKKRWRFWHSFLNCLFLDPKHCYAAYKAEVHRKQYPKAFRLGGI"
     gene            32923..33210
                     /locus_tag="PXD66_000048"
     CDS             32923..33210
                     /locus_tag="PXD66_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318392.1"
                     /translation="MKKQNLKLVRGDDVSFRFSIKDSTGQPLALKDLWFDLHLVSYQG
                     EVLIALSTDDGSIETAEAGQMVLHFSHQQTARAEWTSANYDLQLTHDYTRV"
     gene            33220..33384
                     /locus_tag="PXD66_000049"
     CDS             33220..33384
                     /locus_tag="PXD66_000049"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318393.1"
                     /translation="MTSIIELDLTDDLIIEVQLDSTIITGDSFLANLPDLLQIYNQAK
                     SDSSKELNND"
     gene            33377..34081
                     /locus_tag="PXD66_000050"
     CDS             33377..34081
                     /locus_tag="PXD66_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318394.1"
                     /translation="MTKHKNHENHPIAITFYQLGKDVGDINRRLAVIEQREQSAQPSQ
                     PQAEAVTMPQQDAVAINNINAVLEAVEKYKTFEKYNTQWQERFEKFLSRNPNDYELSE
                     EQRQKLYHTMCQHLWQVNFTEINSKQYELPIPQQFQGKFGYIVKTSGGSTEISFELLS
                     ETHSTYLYPDSYIPEGGGDVMLKPAYIYLVIPDLVAMREPEVVGVSPLKLNILQKHLL
                     TPQKLQEQKPLNTKAN"
BASE COUNT        10821 a         6857 c         8104 g         8527 t
ORIGIN      
        1 taactttcaa ggatctcaca aaatggcaag aaataaaaga gttcgagata tgagtaattt
       61 cgaaattcgt ggtgagctga tgaaaatcgg taagtcctta tctcagttag gtattgagaa
      121 tggattagca aaaaccacag ttcgtaatgc gttagataag ccatatccca aaggggagca
      181 aattattgca aaagcgattg gtaaaacacc gcaggaaatt tggccctctc gttacgaata
      241 aggagctttt atgaaggaat ggtttttagc caaagaaatc gcaggattat ctggagttcc
      301 agaattaccg aatagcgttt caagattggc aaccaaagaa ggatggcaaa aacgacaaat
      361 tcaaggtgta agaggtgtta cttacgaata ccacctcacc agcctaccca ttgaaaccca
      421 acaacaactt agactaaacg ccgcccttgc ggtaataccg caagcggcag aactacaacc
      481 caaacgagac gaccccgcac ttatcgcaag gctcaataat gccaccgata aaggcaggga
      541 taaagcaaaa ggcaaggcag aagcctgtat gcagttgcaa gcctttctcg accaagggtt
      601 tagttatacg caggcagaag cgggagcggc aaccgctaaa aatgtgtcgc aaggttcttt
      661 aaaaaattgg tactacaaag tgaaaggtca ccccgtccat ttatggcagg cgatcttaat
      721 ttccgaaagc ggtaggagta aaaagccgca gctgaaagcc aagattactg aagaagcgtg
      781 ggactgcttt ttggcggatt acctccgccc tgaaaagccg gatttacggg caagttaccg
      841 ccgaacccaa gccatcgcca agcaatacgg ctggcaaata gcaagtttac agactttcca
      901 acgccgagtg ttggcggaag tgccttacga ggtgattttg ctcaaacggg aaggagcaaa
      961 tgcggttgcc aagttagtgc cggcactaca acgcacggtg aaagatattc tcgcaggaga
     1021 atggattaac ggcgatggct atcaacacaa tgtgtttgtg aaatggcaca ccggcgagat
     1081 tgtccgccct aaaacgtggt tttggcaaga tgttcgcacc cgcaagattt taggttatcg
     1141 aacatcgatt tcggagaata ctgacagcat acgccacgca ttaatggatg tgatttttaa
     1201 cgtaggcata ccgaaaacgc tcacgctgga taacacccga gcagcagcga ataaggcaat
     1261 gaccggtggt attgaaaacc gctaccgctt taaacatacc gagttagacc cgaaaggcat
     1321 tatgccgatt ttagggattg atgtgcattt tacctcaatt ctttatggtg aaggacacgg
     1381 gcaagccaag ccgattgaac gtgctttcgg gcgaggcgga attggcgaga aaatagataa
     1441 acgccctgaa ttaagtggtt tctatacggg cagaaatgca caagaaaccc ctgataacta
     1501 taacggcggt aaagacggtg tggattacaa cacattcttg aaagccatag cagcagggat
     1561 agaagagtac aactctcaaa ccgaacgcca taccgaaatg tgccgaggag aattgagttt
     1621 taaccaagtt tgggagcggg attaccaccc aagcaacgta agacaagcca gccctgaaca
     1681 gttgcggtta ctgttcttac aggcagaaac ggtcagcatt aaacgcaacg gcagttttac
     1741 gctgaaagca gccggcaaac tttacggctt aactaacgtt tactgggcag aaagcctgat
     1801 agggataacg gataagaaag tagtggcacg gttcgacccc gatgacttac acggcaacgt
     1861 gtatgtgtac aacctagagg ggcaattctt agccgaagcg gtttgccgag aggcgaaagg
     1921 atttggtgat accagtgcaa gccgtgagca aggtcgctta tatcagaaag tagtgaaaag
     1981 tgccaaagct caagccgaag cccttgaatt actggaagct cacgaactgg caagcctcgc
     2041 cccacaagtg gacgtgcctg agccgataga gaagaaggta aaagaggtgc tggtgaaaga
     2101 agattttatc gtggatttca acactgtgcg taaagcaacg gtggtggaag aaaccgaaga
     2161 gatcagtatt tttgatgaag tgttactgaa acaagagaaa caacttcgca tagtggaata
     2221 actagcggtc agtttttgca aaaaatttgc aaatttttct aaaaaaacga ccgcttgaaa
     2281 tgagatttaa acagggttta aacgccttta aggagtaaaa aatgacatta acagggcaag
     2341 cttaccgagt gattttcaag gaactcaaag agcaacaaga gagtgaattt gaacaggctt
     2401 tcaagaaagg gattgccaaa attcagcaga atcaaactca ggaatccatt ctggtaaacc
     2461 gtgatatgtt tcaagctgaa aagctaacac tgctttcaca aaagttggaa gcggttgagc
     2521 aattacttcg ccttgaatta aatcagccga attttcaagc aaaacttgaa gactacgctg
     2581 acgcattacg aacaaccatt ttaagtaaag tagccaaata agagctttag actcttgaag
     2641 gcttaacatc tgagccactt tttttactgt cggcattata ccgtcaagat catcaataga
     2701 agttaaatga tgaaaagagg aatggtattc agctaaacaa aaattgattg tactaagttt
     2761 aaacaaagta atgcaatttt tcatcacgat ggcatttgct cgttttaact taaaaccaga
     2821 gaaggttgca cgagcagaat caagggctat ttgatgaatt tcatcatctg ttaaagaacg
     2881 aaagagcata aaaacctcca aacaaaaaca ggacaccatt atgacattaa tcgaccaaat
     2941 taaacaacac atcgccgaaa gcggcagttc tcaggggaaa gttgccaaag aagccgggat
     3001 taatgccggg gcgttatcgg cttacctgaa tgaaaattac aaaggcaata atgaagaact
     3061 tgaagcaaag ctgattgctt atttagagcg tattgaggcg aagaaaagag agtttgtcga
     3121 agctccgagt tttattgaaa ccaaaaccgc aaaccaaatt ttcggttcgt tacgatttgc
     3181 tcaaaatact ggcgtgcttg ccattattca cggtgcgagt ggcgtgggta aaacacaagc
     3241 ggctagagag tatcgtaaac gctatgcgaa tgtatggttg gttaccgcaa gcccttctcg
     3301 ttcctcatta agtgaggtgt tatatgaaat tgctcttgaa cttggaatga acgatgcacc
     3361 acgccgcaaa gggacattgg ctcgtttaat tgttcgcaaa atgaaaggca cagcaggttt
     3421 attaattatt gatgaagcag accatttacc ttacgaagcc ttagaagaat taaggctgtt
     3481 acaagaagaa gtagtgaatg aaagtgacat cggagctggt gttggcttag tgctgattgg
     3541 caatgacaaa gtctacaccc gaatgaaagg cggaattaac cctgctcacg aatatgggcg
     3601 actttggtca cgcagtgcca aacgcaccag tattcagaaa accaaaaaag aagataccca
     3661 agcggtagcc aaagcgtggg ggttgagtga aaaccaagaa gcactcaagg taatgcaagc
     3721 cattactgaa actggtggcg gattacggat tctgacacac accttacgat tagcagcgat
     3781 ggtagcaaaa gaccgaatga ttcccttaac tgccgattta attcagctcg cacgtaaaga
     3841 tttactctca acaggagatt aacaatgaga accccaaaac ccaaacacag ttttaaccgc
     3901 accaaccaag cagcgtgccg ttttttaaaa caaacccaaa aggcaattat ccgcttaaac
     3961 agtcttggct ttacggtgct gaatattgat tttacccgta ttaagccccg cattgaggtg
     4021 gaaatcggca ataacaagca cattgctcaa ggcttgattt atgagggtaa agcctaccgc
     4081 tacagcttcg gtaaaagcga agatttaggc agatgggaag gctactacac catgctggaa
     4141 ggtatcagag tgtggtggcg acaagcagca attcactagg agaaaaacag atgaaaacct
     4201 tagcactatt aaccgccctt ttagggcttg tagggtgtga tttagtccaa agcaaacaag
     4261 ctaaaaatta catcggcaac aatttaactg aaatctgtat agataacgtg gtgtacctga
     4321 tttattccgg tgataaaaaa ggcggaatta ccccgaaagt aaataaagat ttttaccctt
     4381 atacctgtac aaacaaagag gaaagacaaa atggcaaaac ccgatgaaac acccattaca
     4441 aaagcagaat gtcaatctca gcttgcagag ttgggggtgc aatataaaaa actgccaatg
     4501 gcaattacaa gacatatttg taatgccaca accaatatac acggcaaagt tataaaggtc
     4561 agtgtggtag agagagttgg atatggcgtc cagattaccg cacaaggtaa cgagaaatct
     4621 tgcttagtaa cctatgaagc aatgttaggt atggcagaag caatgggatt atttgatgaa
     4681 gttaaggagc aaaataatga ctaaaaaaac aaccagagta aaaagtgcaa cccaaagtgc
     4741 gatttatcaa agccgagatg aagtgcaagt ggctataaaa atcattggtg ataaacagcg
     4801 tgaattgcaa cgtttagcta cggcaatgaa tgatgaatta gctgctatta gtgccagtta
     4861 tgctcaacat aaccgctacc aacattatgc cgagaaagcg gctgaagcag aacgcaaagg
     4921 caactataag gaagctcaag accattggga agttgcaaaa ttatcagcaa aaaagaccgc
     4981 taaccgagac tgggcggaac aacgagcgga attttgtaaa cgtatgcaca ataaaccgtt
     5041 ttagggggaa gtgatgacag aaaccgtaaa agcacaactt aacagccaat taaatgaggc
     5101 aattatccag ttgatacaag ctcaaaagta tttaaaccaa gatgatgcca ttcgcagtgg
     5161 agtgtatatc ggtacggtgc aggatttatt gccaaaggtg catttaaagt tattaacggc
     5221 aaatcgtaaa cattgataga gatgaggtaa aaatggctat tacagaagaa caatggaaag
     5281 aaattaagca aaagctagat agtatcatag ggagagttaa atttagatac aaagagcatt
     5341 tactcacggt agatgttgta caaattaagc gatctttaaa attggctgtg tatgttgatg
     5401 gggaaattga tggagcttgg acaaaagaag gacacgaaat tcgcccttat ttagaagaag
     5461 tttggtatcg caaagaacgc ccttttttta atgctaagga gaagaaagag tatagaggtt
     5521 taatgagcaa aaaaaaactg aatgaaaaaa tagtaaccta ctcacctatg tttccatcac
     5581 caactgcact gactcgtcaa tataagaaac tagatggctt agagttgatt gaagtgattt
     5641 aaaacccatt tacagcccat ttaaccccga gttatttaaa ggacaacaaa atgaataaaa
     5701 acgaattttt taagaaatac cacctagagg gttcactagc tattttggat ttggatgcaa
     5761 atgacgatca aagccaattc tgttaccaag cattgttgga gtatagagaa ctcccgttag
     5821 acacatttct tgcagagtac tcagatgatg aggatatgac agagtcagag tttattgagt
     5881 taatgcttga tgaattagag tggattatcc gcagagctaa gaatgctaaa cggtttgtta
     5941 aaaaacgaca aaaaacgatg agggaggaaa ttatgtaaca gctcaaccca accaacgcca
     6001 catcatccgt ctagctgaga aacaggtgtg gcgtttgttt taagtgggca atcaaaagcg
     6061 ataacgtttt taattgttta tttatcggag taacaaatga cagacaaagc caagctaatc
     6121 cagcttatcc atattgccaa gtcgcaacta gcaatggacg atttaagcta ccgtgaaatg
     6181 ctgaaacggc tcaccaataa aaccagctcg accaaatgca cggtggtcga gttgcacaaa
     6241 gtcttacacg aactgcaaac caaaggagca aaagtaaaat attttgccaa acgcagcaaa
     6301 aaaccgaccg cttacagccc ggcaacaggg gaaacagcgg taaaaagcca aattgctcat
     6361 aaaatccgag cggtatggat aacaatgggc aaacacggat ttttaaacga cccaagcgaa
     6421 aaagcattaa atgcttatat gcgtaaggtt atcaataata aaactagacc aacaatatta
     6481 ttaaatgtct ctgcattaga ggctggagat gcaagttata tgcttgaaat cctcaaaaaa
     6541 tggcataaac gtgtgatgat taaagcccta agcaaaagtt gcacagttga tccaaagata
     6601 agctacgatg aattagtgga gtggtacaat gaagttatgt agatgcccga tttgccacag
     6661 tgatatacac ctagaagctc tcgtagaaga tgaagccggg cgtgaactac tcggcaaaat
     6721 cagccaatta acacacggtg ttgctcaacc gatggttgct tatttaggct tatttaaacc
     6781 gcaaaaaagt aaccttaata actcaagagc cttgaaaata ctcaatgatg tattagcact
     6841 ctacccttgc tcattgctac tggctcaagc cctatctgaa acggtagtaa gcatacgcaa
     6901 aaaacgtcag caagcaatgg aaaacgggca gaaaattgaa ccgctaccga accataacta
     6961 tttaaaatcg gtgtatgaaa cgcaaaaagt ccattttgct gtggtgagaa ccggcaaaac
     7021 cgaacgcgaa acaatgaaag ccgaactgga aaaaacacgc aatgcgattg cctatgtgca
     7081 acggtttgtt gatttaggac gtgaagaggt tgttaaaaat agccctgagt atcagatttg
     7141 gttagctcat aaacccaaat aacacaagcc accaaaaggt ggctttttaa ctttttgaaa
     7201 gtgcgataaa acctgattta aacaggcttt aaacgggttt taagattgag tttaaaaaaa
     7261 tatttcacaa aataagaatg gttttttata caataaccga aacggttttt ggataggaaa
     7321 tagacaatgg aattagtgga tatttttgaa gaaaaagccc cggaagtgtt aaccgattta
     7381 gccaaacata ttgaagtcgc attagaagaa aaatgccaat ttagccccga aaaggcaaag
     7441 caaatcggta ttgatattgc ccaacaaatg gctcaaaact ggggtggcga ggtaatttat
     7501 attcctcgca atttgttgat ggctcttaat gagcgagata tgaaaattta cgaagagttt
     7561 aacggtaaca atcaccgtga attggctcgt aagtacaatg tatcaatgca gtgggtatat
     7621 aaaattgtga agaaagtcca taaggacgaa gttgcccgca ggcagatgag tatgtttgag
     7681 atgtaaatca ttttttccca gccctgatca ttttcagggc tttttgtgta ttaaaagaac
     7741 gcagtcttta aacccgttta aaatcaattt ttcagcaaat tctttaaact ccttttaaag
     7801 caattttaat aggagtgtgc tatgtctcaa aatcttacct ttcgccaaat ttttgaccgt
     7861 ttaatcggac acgagggcgg ctatgtcaat cacccacaag acccgggcgg agaaacccac
     7921 tggggaatta ccaaacgtac cgcggttgct aatggttata tgtatgcaat gaagaatatg
     7981 acccgtgagc aagcctacca aatctacgaa aaagccttct ggcaacgtta tcgttgtgca
     8041 gagcttaagc cagcagttgc ttatcaattt ttcgacgcag tggttaatca cggttttggt
     8101 aatgctagtc gtatgttaca gcgtgcagta ggcgtagcgg atgatggcat tattggcaat
     8161 attaccctgc aggcaattaa tgcaaaatct gaaagcgatc tcttaatgct atttaatgcc
     8221 gaacgtctga agttttacac aaaattacgc aacttcccca cttttggtaa gggttgggtc
     8281 aatcgtgtgg caggcaattt agaatacgca gcacaagata atgaggttta aaaatggcaa
     8341 aactatttaa agaactactc tcaaccaatg gcacattatc caccactagc accgtgcagt
     8401 tttttggcac attgtgtgta tttggtttaa tggtttatgc agtaattacc caacaacctt
     8461 atgccgaaag cctattaaat aatgtgttga tttatctttt tggggcaact accgctaagg
     8521 gtgtagtaac cagctatcaa gccaaaatta aagggggtat ccataaacaa caaagtacat
     8581 ttaaagagga ggagggatgt taatgcaaca aattttaggc gtgattggcg tcatcgcagc
     8641 aatattgtct tatgctttgt ttaaaagctg gcaactaaaa cgtgagcgta aaaagaatgc
     8701 catattacaa gctgaaaaac agcaacaagc ggttgaaatt gagcaaaaaa aagcagaggt
     8761 aaaatatgtg tatatatcaa agaaaaacaa tgaaaaagtt actcgcagct ctgctagcga
     8821 tattgatgac aagttgcagc aacacggtta cttccgtgac aacaacaggt tgcacggcgt
     8881 tcagtctgat ttatccgaac cgtgcggata cgctggaaac gaaacgtcag attcttgctc
     8941 ataatctgac ttttgaggaa atttgccaaa atgaaataca ttaaaaaatt acgtaaacgc
     9001 tggcaaattt ggcgtttttt aaaaaatcat cctgaggttg cacaacgccg agatttatta
     9061 gcgattgcca ttaaacaggg cgtagaaaac cctgtagggc gtgcaagacg gagggagcca
     9121 taatggattt atttacagtc ttttgggagt ctatccgtgc taactttggt tttatttctg
     9181 cgttactcac cattgttgtt acggcatttt ggctcaagct agatagcaaa tacgccaaga
     9241 aaaatgatct tggtgaattg ctggaaattg ccaaaagcca cgataaccgc ttaaccacct
     9301 tagaaaccaa ggtagaaaac ctgccaagtg cggtggacgt ggaaaagctg aaaaccttag
     9361 tcactgatgt aaaaggcgat accaaagcca cctcaaagca ggtggatagt atcagccacc
     9421 aactcggctt gttattagaa gcaaaattaa aggaatgata tgtcgttaag agatttactc
     9481 actcaagacc aacggctggt gattttacgc tcattagcgg aagccggcta tgatgccaat
     9541 gagtcaatta ttagtgattg cctagattta tacggtcacg atattagccg tgatttagtt
     9601 cgcacccatt taagttggct tgaagagcaa ggcttactta ccttagaacg tttgcgagat
     9661 ggctatatgg tggcaagcat tacccagcgt ggcttagatg ttgctcaagg gcgtgttgtg
     9721 gtaagtggcg ttaaacgccc tcgccctaaa atttaaacgg tatttaagga gcgtttaaat
     9781 gagcgagaaa aatactcgtg gtcgagcatc aaaagtcgat ttactgccgc cgaatattaa
     9841 aacccaactg gcgatgatgt tgcgtgataa gcagtactct cagtcacaaa ttttagaaga
     9901 aattaatgac ttaattcggg attgtggctt gcctgatgaa atgctgttaa gcaaaaccgg
     9961 attaaaccgc tacgcaagcc gtatggaacg cttaggggca aaaatccgta atgcccggga
    10021 agttgccgaa atttggacta agcaattcgg cgaaatgcca caaaccgata ttggcaaaac
    10081 cctaatggaa atggtcaagc aaattgcctt tgaaacctca ttgaaattag gcgaacaaga
    10141 aggcggtatt gagccaaaac agcttgcttt gctctcgtct gctattcaac gtttagaaca
    10201 agccgaaagt ttgagtttta aacgggaaca ggcaatccgt aaagaggtcg cacaacaagc
    10261 cgctgaaacc gctgaaaaag tggtggtgca agcgggttta tccgctgaaa ccgtccgtac
    10321 gattaaagag caaattttag gaattgcata atggcactac tcaataacag accgctgaat
    10381 gaattagccc ctgaatgccg ttcgttttta gagagcattc acgcttttaa tcctaacgaa
    10441 ctgttattgg gctatcaaaa gcggtggatt gcggacgaca gccaactcaa aattgccgaa
    10501 aaaacccgcc gttgcggttt aacgtgggca gaagcagcag ataacgcctt aatcgcaagc
    10561 acccgcaaat cagacggtgg ctctgatgta ttttacattg gctcaaataa agagatggca
    10621 cgtgaatata tcgatgctgt cgcaatgtgg gcaaaagcct ttaatcgtgt ggctggagaa
    10681 atccaagaag agatctttga agacgaagat aaagacatct taacctatgt aatctacttc
    10741 tcatcgggtt ttaaggtcaa ggcattatcc agtaatccga aaaacctgcg tggtatgcag
    10801 ggcgtggtgg tgattgatga ggcggcattc cacgaatact tagcggaagt cttgaaggcg
    10861 gcgttagctc tcacaatgtg gggggcgaaa gtccgtatta tttcgaccca caacggctcg
    10921 gaaaatctgt ttaatgaact gattaccgat agccgtgccg gccgcaaacg ctactctgtg
    10981 catacgatta cgattgaaga tgcctgccgt gatgggctat atcaacgtat ctgccaagta
    11041 gtgacgaaaa aaacgtggtc accggaagca gaaaaagagt ggattgaaaa cctactcaac
    11101 gacaccgcca gcgaagaaga cgcattggaa gaatacttct gcgtaccgaa gttcggctca
    11161 ggcttatggc tctctcgtgc tttgattgaa cggcagatga gcgaagccac gcctgttatc
    11221 cgctttacgg caaaagatgg tttcagcctt gtggctgaac cgacacgcta tgcggagatt
    11281 gccgaatggt gtgaattaga acttgagccg atattggcaa cccttaaccc tgatttgttg
    11341 cattttatgg gcgaagactt tgcacgcagc ggcgatatga cctctctggt ggtgcttgct
    11401 cagcaacaaa accttgttcg taaagtcgcc tttattgttg agcttggcaa tatgccctac
    11461 aagcaacaag agcaaatcgt gatgtttatg cttaaacgct taccgcattt tgccggtggg
    11521 gcttttgacg cccgaggcaa tggtggctat ttagccgaag ctgcacgaga tgaatttagc
    11581 accttgattg actgcgtgca gttatcggaa aaatggtatc gggaacacac cgcccccttt
    11641 aaagcggcat tggaagacgg cgagctggaa gctattccaa aagatgcgga tattttggct
    11701 gatttgcgtt catttcaggt ggtgaaaggc gtaccacgca taccggataa acgcactaaa
    11761 agtgcagatg ggaaaagcaa acgacacggt gataccgcaa tttctttgct gttggctcat
    11821 tatgccagcc gacaattagt gcaaatgcca gttgagccgg tatcacgccg tccacgccat
    11881 agccaacaat taacagaagg atattaacaa atgagatatg taccactttg tttaatgatg
    11941 tgtgcctcat tctacctatt tattaacaac attgacggct ggggttgggg aattctttta
    12001 tcttttattt tagcgagtgc agcttatggc aaagaagaat aatttaatta gtgaaattgc
    12061 cacccgtgcc agaagtattg attactgggc atttggctat tatctgccga accctgaccc
    12121 gattttaaaa cgaatgggga aagatatttc cgtttaccgt gagttgttat cagacgggca
    12181 agttcgctca ggcgtacgcc gtcgaaaagc ggcgattaaa ggcttagagt ggcgaattac
    12241 caccactaac aatgagaaag tggacgagca gatttatcaa atatttaacc gcttgccgat
    12301 gaataacctg attaccgaaa tgctcaatgc agcactctat ggctaccata tcagcgaggt
    12361 tatttgggca aaagagggtg agttatttgt gccaacagaa atcattggta aaaagcctga
    12421 atggtttatg tttgacgacg aaaacctact gcgtttccgt accaaagaaa actgggcgga
    12481 cggcgaatta ttaccagatg ataagttctt gcttactacg caagaagcca cccaagataa
    12541 tccctacggt ttaggcgatt tatccctctg cttttgggcg gcgaccttta aaaaaggcgg
    12601 tttcaaatac tggttggaat ttaccgaaaa atacggctca ccgtggttga ttggcaaaca
    12661 ccctcgccaa gctcacaata cagataaaga gcgactagct gatgccctag agggaatgat
    12721 tagctcagcg attgcggtta tccctgaaga tagttcggtc gaaatcaaag atgcagcggg
    12781 caaatctgcc agcaccgatg cctttgaaaa gttcctcaat ttttgcaagg cagagatcaa
    12841 tatcgcccta cttgggcaaa accaaaccac cgagcaagaa agcaaccgtg ccagtgcaca
    12901 agtaggcttg gaagttatgg aagatattcg ctcggacgac caagcgatga ttgaagccac
    12961 cttcaacacc ttgttgcagt ggattgtgaa atataacttc aatgtggaac aactgccgaa
    13021 gtttgaattt tacgagcaag aagccatcaa caccgaccaa gttgaacgag acagtaaact
    13081 ccacgcaatg ggcgtgcgtt tttccaaagc ctactttgag cgtgaatatg gctttgaggc
    13141 gggtgatatt gaggtgcaag cggtcgaaaa agcggaaaaa tctgcaaata ccacagaatt
    13201 tagcgaagga caacgccgaa cccaagtgcc aatgcctgtg ccaattttgg aacagctaga
    13261 acacgaggga gaagcaatgg tggacgattg gctacaaggg gtgagagata aactcgctca
    13321 agcagatagt ctggaggatt tccaaagtca gctagatagc cttatccctg aattaagctt
    13381 tgccgaatat ggcgagctgt tggcgttagg ctcaacggtg gcagagcttg caggcagaca
    13441 atcggtaaac gatgaaatta aggcaaaacg caatgcgtaa atttaccttt aaacagcaag
    13501 cccaatattt tgagaaaaaa ctcaatctcc gcacgaatag ctaccttgat attctgggcg
    13561 aagagcacga ctattttttc gtggtggctg gggcgaatcg taatgaagtg ttgagtgagt
    13621 ttcgcaaggc ggtggatgag gctattcaca acggcgaaac cttagagaac tttcgcaagc
    13681 ggtttgatga ggttgtggca aaaacaggct gggagtacaa cggcggacga aattggcgaa
    13741 cacggattat ttacgatacc aatgtttacg gggcgtataa ccgtggacgt ttgcaacaac
    13801 atttagagct tgcggaggat atgccttatt gggaatatca acacaacgac aacgcccacc
    13861 ctcgcccaca gcacgtggcg tgggacggct tggtgctgcg ttatgacgat ccgttttgga
    13921 aatatcacta tccaataaaa gcctacggtt gccactgcac cgtgcaagcg ttagatgatt
    13981 ttgacctcaa gcacgatggt aaaaccgtca gcccaccacc tgaaattgag tttgaacaaa
    14041 aaacggtagg cgtgcggaca ggcaattcac gagtcgtaac tgtgccgaaa ggggttgatg
    14101 tgggttttac accgcataat tttgataatc tcaaagccgg gagagcagca aatgtagata
    14161 agttgctaat gcaaaaaatg gttaccgccg atcctatatt tgcctccaaa gcagtcagtg
    14221 atttattggc aaaagagccg gttgcaaagt tacataacca agcctttaaa aattgggtaa
    14281 atgatgttgc caatgctaaa ggaagtgcat tatcccaagt agagaatatg cagttgttag
    14341 gggtactacc tgttgcggtg attgaacggt tacagcagat caatttagct ccgcaatctg
    14401 caattattgc aataacaaaa ggcgatatgg tacacgctct acgagagcta aaacaaaata
    14461 ccggtattgc cgtaccgttg gaattttggg aaaagttgcc tgcaaagtta caatacccaa
    14521 atgccatctt gttggaaaaa caacaagcag aaactacatt gctctttatc tatgacgtag
    14581 ctcaaaccaa aattgccgta aaagtcaatt atgatgcaac tttacaagat agacacggag
    14641 caaaaggacg gcagaaagtc ttactgaatc gagcggttac aggtagttta ttccaagata
    14701 caactggatt gaaaagttca aagtatgaag tgctattggg agaaatgtag cggcgaagga
    14761 ctcgaacctt ataatgtagg ttaaacctac aacctttacc aattaggaaa ctaccgctac
    14821 attgtgtaaa ttcactatac ccctaaatta ttttttaatc aataggagaa acaaaatggc
    14881 agaaaaacaa ctttccttta aagtagccga aatagtgcca atgtcagcta ttaacactat
    14941 gcggcactta attagtttgg cattacaagc gaattatgag agagaaaaat ctcgtgcttg
    15001 ataatttcaa tggcttgtag ttcgggaact atattatctg aaacaagcca ttgttttgtt
    15061 gatgtcagac atttagcgat gaaatccata attaaaccat tagcagtagc tcgttgttcc
    15121 ggagtaagtt gtaaccacga cgcctctgtt tgttggtcta atgtataaag aaacataacg
    15181 gcttcttttt ctgataatgg gtcaagtggt aatttttcag cttttgtgac taaccattga
    15241 cgggaaagca ataaatcata aaccagttct attgcttgtt ctgcatttac catcatattc
    15301 taattcctta atatatcagg gcaacattac ccaatctata ttttactgag atttgcttaa
    15361 tgatcaaaat caccctcaac aacacccaag cggtcaaatc gttacaacaa attgcaaatc
    15421 agctggaaca acctcgccga ctgtatggca ttttagggga aaccctaaag aaaattcaca
    15481 ctgcacgctt taaagcggaa caagaccccg atggcaacaa ttggaaaccg ttagccagta
    15541 gcacgcttgc tttaaaacgt aaacgaggta agtccactaa aatcttacgg caagacggct
    15601 accttgccga taaaacggct tacaatgtta caagtgataa tgttgagttt ggctcgcagg
    15661 aagtgtatgc acggttgcat caattcggcg gtaaagccgg acgaggcaaa aaagtgactg
    15721 taccggctcg cccgtggtta ggcgtaggcg ataaagatga gcagttactt ttacgcaaag
    15781 ctgagtggca tttagggcaa attatcggtc gtatgggaaa ataaccgctt aaaatcaaat
    15841 ttaacgcata aaaacgcctc tgtgcgtttt tattttttat tcgcctgatt attcgactca
    15901 aaaaatttaa acgctgttaa acgcatttaa acggtattta aacggtattg ccgattgtga
    15961 taaatcctct ttattaaaac accctcgaaa aatctttaaa ccagtttaaa agctaatttc
    16021 tctccaaatc cttattattg ccccatcacg gaggcaatat gacccaaatt gaaattttta
    16081 aagccggtaa gcgttatgac gccaacggca cgttaattga cattacccct gaaatgttgc
    16141 aacagaccgt tgctgcctat aaccccgaat ttcacgaagc tccgctggtg atcggacacc
    16201 cgaaatccaa caaccctgcg tgggggtggg taaaagccct ttcccttgag ggcgatgtac
    16261 taaaagcaga agtagatcag ctagacgctg agttttcaga aatggtggca agcggcaaat
    16321 tcaaaaaagt ctcggctgct ttttacttac ctgatagccc gaataaccca cataaaggcg
    16381 tgttatcgct acgccacgtg gggtttttag gggcaatgcc accggcggtt aaaggcttaa
    16441 agcaggtgga atttgcagaa gatgatgatt ttgtcgtctt ttctgaatgg ggggttgcaa
    16501 gtctatttag tcgttttcgt gattggttgg tgagtgagtt cgggcttgaa aaagccgata
    16561 aagctcttcc cccacacgaa attgattggc tgaaagaaga ggcaatgcga agccaaatta
    16621 gcaaacaggt acacgccgaa caggcaacac ctgaaccgat ttttaatgaa cccgcacaac
    16681 cagaaggaga acctgaaatg agtgcagaag agaaggaaga gcttgaacgc ttaaaagcgg
    16741 aaaacgagca gttgaaagcc gaaaaagccc aagccgaagc agagaaaatg gaagcagagc
    16801 tggaagctga aaaagctggt aatgcagagt tttgtgaagg tttagtcaag caaggcaaac
    16861 ttgccccggt ggcaaaagcg gcgtttgtgc aggctttaaa taacctttca gtattaaaag
    16921 ccggtcgtga gccggaattt aatgagggcg aagatgtggt ttcacagttt aagtctgcaa
    16981 tgtcgcaatc accacagatt atccaatttg gagaatatgc cacagcagat aaagccaacc
    17041 cacaagaagc ggaagcagtg gaatatgctg aaggcacaga tccagtttcg attgaagccg
    17101 accaaaaagt gcgagcttat atgaaagccc acaatgtagg ctacgctgcg gcatttaatg
    17161 cgatttatcg ctaatgttta acccacaact gaaaaggaat actaatgaca actcataact
    17221 tacaaaaact gcgtgtacaa gaccccgttt taaccgagct ggcacaaggc tatcacaata
    17281 acgagctggt tgccgagacc ttaatgccga ctgtcgaaat cgaaaaggaa gcaggcaaaa
    17341 tcccaacttt cgggcgttta gctttccgat tgccttctac cgtgcgtaac ttgcgtggtt
    17401 catctaaccg tttagatcca gaagatattg gggcgattga tgtggcgtta gaagaacacg
    17461 atgtggaata tgccattgat tatcgtgaag agaacgaagc cattttttct ttacgtcaat
    17521 ttgccttaaa taccacccaa gatgtgattg cgttaggtcg tgaaaaagcg gttgcaaccc
    17581 tcgccttaga tgaaagccgt tatgaaacag acaataaagt cacgctgtcc ggcaccagtc
    17641 aattcagcca taaagatgct gatgtgttta aggtatttga taccgctatt cgtgccgtga
    17701 agcgggctat cggtcgtaaa ccgaatgtgt gtgtgatttc cggtgatgtg tgggcagcgt
    17761 taaaagaaca tccggcagtg gttgaaaagc tcaaatacag tcaagtagcg attgttaccc
    17821 ctgaggtgtt tgccaaactg atcggcattg agaccgtaaa aatcggtgaa gcggtgtatg
    17881 aagaaagcgg tcagctcaaa gatatttgga gcaaagatat tgtggtggcg tatgttgctc
    17941 ctcgttcgac cggaggcaaa ggcacggttt acgagccgtc ttatggttac acggttcgcc
    18001 gtcaaaaagg cttatttgtt gatacttata aagagagtgg cggcaaaatt gaagtggtac
    18061 gcacgaccga tattcacaaa cctcatttag tgggtgcggc agcaggctat ttaattaaag
    18121 ctgccgtata agccacatac catagggtgc gtttactaac gcaccattat ctcaaacaca
    18181 ttacatagag gaaaaaacga tggctaaatt tattgtttta cataccccaa ttttacacga
    18241 tggcaaacgc tatgaggtgg gggcagaaat tgaactcacc gctgaacaag ctgagaacaa
    18301 tgcgatcaac ttaaaagcgg tagaagctgt tccaaaacca cctaaaaatg cctcaaaacc
    18361 tgaagatgag ccaaaacccg aaggtgaacc taagcctgaa gatgcagtga cggaagatga
    18421 tgtgaagctg gctgaagcaa aaattgcaga agcagagcaa ccaaaaggga ataaaaaata
    18481 atgtatgtgc agattgatga gctactcacg gcgtttagtc gcaagattgt ggtgcagctc
    18541 agtaatgatg aaccgacagc gaccgagcct gatttaaaga tgcttgaaac ggcagttaaa
    18601 gtggcaaatg agcgaattga tgcagcgtta cgctctcgct ataccttgcc gctggctgat
    18661 gtgcctacgc ttattaatca gcacgcatta acccttgccc gttattggct ttatgcccgc
    18721 cgtcctgaaa ccaaaatgcc ggacaccgtg aaggaaacct accaacaagc ggtgaaagag
    18781 ttggaacaga ttgcaacagg caaactgcat ttaggcattg ctgaaagcag tcaaatttta
    18841 gaagacgatg ttttagccga taacagtgaa tatgaagttc gggcgaacgc tcgcattgat
    18901 accagtcgtt attaaagatt atgtcagcca cacaaccgat tttagaagat atttgccaac
    18961 acttgcggca agcattgccc gagtgggagg tggaactctt cccgaatgac ccaagcacct
    19021 atcatctctc gcacattaac ggtgcggtgt tgattagcta tttggcaagc aaatttgaga
    19081 aacctcgcac tacacaagcg gtgctgcagg ttcgccacgt tcagattgct ttaacggtgc
    19141 tgacccgtga tttgcataat gatgtcgggg cgttggattt gctggataaa ttgcggttat
    19201 tggttgtggg gtttaagcct caaaactgcg gtgagtgttg gttgattgat gaatttttta
    19261 atggctcaag cgaagaaacc gggctttggc aatatcagtt gattttgcaa accgaaactc
    19321 agcaggtgca acaccaagcg gtcgaaaatc tgccaaaatt tgtaaccgct catcttcgcc
    19381 gtcaagacca gcctggttcg cccggactta aaacttaaat cgtagggtgc gtttgctaac
    19441 gcaccatcaa catcacctga ttttacggtg cgttggcaaa cgcaccctac aaggagaata
    19501 ttatgacgtt tcaccacggt tctgaaaccg aacgagtaaa cggtggctct gtgccggtat
    19561 cggtggttga tagtgccatt atcggcattg tcggtactgc cccaatcggc gcagttaatg
    19621 aattgaccgt ctgtttaacc aaaaaagatt ttgccaaatt cggcacgatt ttaaaccaag
    19681 gcttcacctt acctgatgcc tttgatattt tagctcgtta cgcagcgggt aaagtgtatg
    19741 tggtgaatgt gcttgaccct tcccgccacc gtactaccgt gaccgatgaa gtcctcacac
    19801 tggatcgcaa tacgctgacc gctcaaacag cgaaaaccgg cattattgcg gtgacgagag
    19861 tgaaagacgg tgcaaacgtg ctgaatgctg atcggtattc ggtcgatctg caaaccggcg
    19921 tggtgacctt tgtctctgca ccgtctgccc cgaaaattag ctatatctat gctgacccga
    19981 gcaaagtgac cgaagaggac attaaaggcg gggtggatag cttaaccggt aaacgcaaag
    20041 gttttgaact attgcgtgat ggttttaacc tttacggtgc ggatgccaaa atcttaatct
    20101 gtccggaatt tgataaaacc gcaagctgtg ccgctgccct tgccaccctt gccgaccaac
    20161 tgcacgctaa ggcgtatatt caactgccga aagggacgag cttatcaaaa gctattcaag
    20221 gacgtggccc ggcagggacg attaacgcct cggcaagcaa tgagaacgta cgccatttct
    20281 tcccgtatgc gttggggtca agcaatactc ttgaaagcct tgcgacccac gccgccggtt
    20341 tgcgtatgtt gattgatgtg gagaagggct actggttcag cacttccaat aaagagctga
    20401 aaggggtgat cgggttggaa attccgctga cggctcgggt ggacgataag caaagcgaaa
    20461 ctaatctgct caatgcggta gggattacca ccattttcaa tagttttggc acaggcttcc
    20521 gcttatgggg caaccgttcg tctaacttcc cgactgtgac ccatatcagc aactttgaag
    20581 tcgcctctcg cacaggcgat attattgatg aaagcattcg ccaagccgaa ttgcagttta
    20641 ttgatttacc gattgatgat gcgttgctgg atagcttcct tgaaaccatc gacactttcc
    20701 tgcgtacgca gaaatcgttg gtaggttatt cggtgagctt agactatgaa tatgacttgg
    20761 ttgatgcatt cagccgtggg caagtcccga ttgtgtatga atacactcct aaaattccgg
    20821 gtgaacgtat cagcaataaa tctgtgatga cccgtaaata cttggcaaac ttggtgtcgc
    20881 aacgataagg ggaaataaat gagtattgca attaaccaaa ttgtaaacgg aaatgtttac
    20941 attaacggca acagccaaat ggggcgagtg aatgaggtca aaatccccga tattgagttt
    21001 gaatttatcg aacacaaagg ccttggaatg cacggtgtga ttaaattacc tgccggcacg
    21061 aatgcgattg aagcagaaat cacttgggac agtttttatc cggaagtgcg tgcggtaatg
    21121 cttaatccgt ataaatacac tcagttgcaa atccgctcta acctgcaagt gtttaactcg
    21181 cagggcttgg cagccgaaga gccgatggtc accattatga atgtgtctgc cggtaaaatc
    21241 ggcggcacag ggcataaaaa caaagaaaat gccgaatttg ccgacacgtt aaatgtacat
    21301 tcaattaaac agaccgttgc cggtcgtgag ctgttattta ttgatgtgct ggcgaatatc
    21361 taccgtgtga acggggtgga tgtgttgcaa aaattccgca ccaatatcgg ttagtaaaat
    21421 ctttaaagcc gtttaaaatc aatttaaaca ccgtttaagt aaactccttt gtgaagttaa
    21481 acaacaacca ctcacaaagg agttttttta tggctaacgc agctcaacaa gtattaacca
    21541 atcttcgtgt acaaaccact tataaattgc aataccctat cgctttacca gatggtagag
    21601 ttattgaaac cttagatgta cgtcgtccaa aaggtaaaga ttttcgtttg cttgatgaaa
    21661 aagggtttga ccctgaaaaa gatggcgtaa aaattctacg cttctatatt caacagctca
    21721 ccacacttgt gccggaagat attgatgaac tcgatgctgc cgatattaag ggcttaaata
    21781 acctattgga agatatgctt attgagggaa agtccgacaa ataagtgaac tagataccgt
    21841 ttatgctgat ctcgcttggt ggtttaagtt ttcaccaagc gagcttttag aattggattt
    21901 agtggatatt ccgaaatggg tagaacagat gaacaggcaa ataaaagccg gttatgggca
    21961 gattttacgg tagagccaaa taaagggttc ggtggctaaa gtaagtaaaa tggcgacgcc
    22021 ccaagctaga tacacaccaa cccacgccaa gattgtcaaa atcaatccat aaatgagtac
    22081 aaaactaacg ccctcgctta ggctttgtat agattgaatc gcatctaacc agtagaagat
    22141 actccaaccg actaatgcta ccgctaacaa ccatttagcg gtgcattctg ttcttgttac
    22201 acaagcctcg aaaccagccc actcagtaat tcgattattt aatttagtca gcatacacca
    22261 cctccttaaa ctttccctat tttacgctaa ggatttaacc aatggcaaat aatttagcta
    22321 tcggaatggt aattggtgca agcctatcat caggttttca cggtgctttt tcaggagcaa
    22381 agaaaaagct agaacaatta gaacaggctg tcgctaaaaa tcagcaaagc cattctaaat
    22441 taggttctga gcttactgca ttgcgtagta aacaagctac tctctatgca gaaatgagcc
    22501 gagccagtag taaaggtggt gctgggcttg ctcttatgca acaggaatat aaaaaaattg
    22561 gcaaagaaat tgccaatatt agccgagagc aacgcaaatg gagtacagaa ttagaaaaat
    22621 ctattgctaa gcaaactaaa ttacgcaatg caatggaacg tcatacacaa gctaaagaaa
    22681 accgagacga gcttaaaggg aaaataattg gaacggtagc ttctgcggca atgggtgtcg
    22741 gcgtgatgaa aacctatatg gagcaagaag aagctgcgaa taatcttaaa atttcaatgc
    22801 tgaaagcaga tggttctttc gggaaatttg aagagattgg caagattgct gacacattag
    22861 ggacagattt accgggaaca aaaaaagatt tctacaagct cgcaatggca ttaaagaaac
    22921 aaggtgtttc tgatgatgtg ctgaccggag gggcattaaa aacttctgcc gaactgaatg
    22981 tcttgcttga tatggatcag gaaagtggtg gagagttttt agcgaagttt atggaatctc
    23041 accggctcaa tgaaaatgag ttaccacaag cagccgatta tctgcaacga gcaatgtttg
    23101 ccggcggttt aagtaaagag cagatgtatg aatcgatgaa atactatgct ccaaaactga
    23161 actcaatgaa acttacaggg gctgagaata ccgaaaaagt gctggcgatt gaagcaatgg
    23221 caggtcagca aggcttagag ggttcaacct ttggtacggg cttaaatatg atgttaagcc
    23281 gtatgaataa aggaccggaa atgctcaagg cagcagccaa aggaatgaaa gccgaagctc
    23341 gagagatgat ggaggctgtc ggagtagaat ttaatttctg ggataaaaac ggctcattca
    23401 aaggcattga tggaatgctg gctgaaatga cgaaatttga aaaaatccgc aaaaaattcg
    23461 gtgacaaagg tgtaggttta gttgcagaag agctgtttgg cattgaaggt gggcgtcttg
    23521 ctgatatttt ggctatgaaa ggtcaaaaag gcttagatga aatgctgagt aaaatgcgtg
    23581 aacaagccag ccttcaagat cgcattaacc ttaaaacaaa tacattaggt gctgccctag
    23641 aatctttagg aggagcgtgg gaaacctttg tgggggatat tggctctgtc tttgccgaag
    23701 atataaaagg gttttcaaaa ggcttgcaat ctgcggtgga atggcttgtg ccatttgtat
    23761 caaaacataa agaggcgatt aaatttgtag gtggtttggt tgctggactt gttattttac
    23821 gaggcgtttt acaagctggt tcttttgtta ttcaaacggt aactatggca atgaacgccc
    23881 tcaaaattgc agcaatgacc aatcctattg tcttagtcat tggagcaatc gcagcgggtg
    23941 cttacttgat ttatagccat tgggatagca ttgccccttg gtttgcaaat ctttggcaaa
    24001 atgtgaccgc ttacttcaat caattttgga cttgggtaaa aggtgtttgg tcgggcgtga
    24061 cggactgggt gtcttctgtg tggaatggtg tagccacttt tttcgggcag ttatggacta
    24121 cgattactag cttatttagt gtagcttgga caaatatcca aacaatatgg ggaggagcaa
    24181 cagcttggtt ctctggttta tgggatggga ttaagtctgc ggcttttggt atgttcgaca
    24241 gtattagcca aaaagtcagc tctgttgtag caagcattaa gagcttttta ggctttggcg
    24301 atgatgtgga taaaatgggg gcaaaaattg gcaatgtagc tacccaagtc atcagcaaag
    24361 aacactcagg acaactcagc aaaaccgcag atatggcagc caatacaggg ttctctagcg
    24421 gtggtttcac cgggcaaggc ggtaaatata cccctgccgg tattgtgcat aagggcgaat
    24481 atgtgatgac gaaagaagcg acctctcgtt tgggtgttgg aatgctggat agcttaaact
    24541 atggcggtgc tatgggtttc actcaacctg ttttgcaacc gattttaggt aagcaaaaag
    24601 gcttttttgg ctcactttgg gacgatgtga aattcggggc aaactttgtc ggcaatctgc
    24661 ttggcttaaa tcaaggcagc ctgaaaacgc ctgattttaa cccaaatgcg accaatgcaa
    24721 gcggtcaaaa ttcgtcgatt tttagcaatt atgaaccact caaccgaaat gcggtaacac
    24781 agcaggaaag ctcgcaagca ccgcagatag tcgtgaactt taacccgaca atcaatgtaa
    24841 acggtaatgc tacgcaagag gctcaatcgc aaattatgca gcagttgcaa atgagtgctt
    24901 atgagtttga gaaattgctc aatcgtgtgc tagaccaacg aagccgtaga gcctattagg
    24961 agtgatttat gtattttatg ttaggcaata ttgcctttga gccggtgaat ttaactgaat
    25021 ttagcgagca acataccgcc gaatttgccg aacacgctgt gctaaaaggc aaacctcgtt
    25081 tacaagcgat gggcgaaaag ctaaatgaac ttaatttttc tcttcggttg caccatacca
    25141 taggcggtgt agagagccgt tatcaagcct tgcttgaagc caaagcgaag caagcggcat
    25201 tggcgttaat ttggggcagt cgttataagg gagattatgt aattgtcgat atttcatcga
    25261 ccacattgtt caccgatggc agaggcaatg ccctcgctcg tgaaatgcaa atcagcctga
    25321 aagagtttgt gggcgataat gacggtggtg tgttgggtga ggcgttaaat tttggtggtg
    25381 gctcactgct tggctctatt ttgccaagcg gtgcggtaac taccctatct caaatcaaaa
    25441 cagcagtcag ccgaggtgtg gagctttata acagcggcaa acgcttagtt gacgaagtgc
    25501 aaaataccgt ggcgattatt cggcagttga aaaatgaccc ggcaactgca ttggcatatt
    25561 tacccgctgc attagcgaat ttagacgggg cattggggca ttttggtaat ttgaccggct
    25621 catcggcgac cttgggcggt ttgcaggaga aactaccggc agcagtggtc tttagcgagg
    25681 aaatcggcga gatttatcag agtttgcaaa caatgcgaag cagtttgggt aatgcctcgg
    25741 caaatagctg ggataattgg tttacaccgg cagagagtgc attgacagag gtcacagaca
    25801 gctttgacca tctagcaaaa ccggtggcaa aaatgaccgc ttggattgtg ctgcgtaccg
    25861 atgaggaggc agaaaatgac acagttgctt aaacatatcg tcaaacaggg tgagcgttgg
    25921 gataacttga gctatcaata ctacggcaat gcgtttgaat atggtcgaat tattgatgcc
    25981 aatccgcata tcggtttttg cgaggtgttg ccgatagggg taacaattta tatccctgta
    26041 cttaacgtta agccaacgaa taatgaaaat atgccaccgt ggctaagggg gcgtgatgcg
    26101 taatgtaccg aagcccgatt tttcactgtt ttatgaaaaa accaatatca ctgccgagat
    26161 tgagccacat ttggtgcaac tgacctatat cgaccatttg gaaggtcaat cggacgaact
    26221 cactgtggaa tttgaagaca ttcaaggcaa atggatacgc caatggttcc cgactcaggg
    26281 ggataagctc aaagcggcga ttggctatca gggcgaattt ctgacggaca tcggtgagtt
    26341 tgaaattgat gaagtggaat atagttataa gccttccact attaacttaa aagctctaag
    26401 caccggtatc agcaaagcca accgtacttt aaaaccgaaa gcctatgaga atacgactct
    26461 tgctcaggtg gtggcaaagg tagcagataa cctgaaatta aaggtagtcg gtaagattaa
    26521 ggctatcccg attaaacgga tcacccaata tcaagagcgg gacgtggagt ttttggcacg
    26581 ccttgcccga gagtatcatc acagctttaa aatcgtgggc aatcagctgg tctttaccga
    26641 taaaagcgaa ctggggcaaa gtgagccggt gttaatgttg gaagaacggg atactatttc
    26701 cctacgtttg cgagatcgga ttaaagacac tgcaaaggca gtggaaatca gcggctatga
    26761 tacaagcggt aaaaaagtgg tgaaaaaacg caaaaaggca aaagccttac gcccgaatat
    26821 aaaacaagct aaatcagcca gtgaagatac gctgaaaatc atcacacgag gcgaaagtca
    26881 agaacagatt gatgcacggg gtgaggctgc gttggctacg caaaatgaag atcaggcggc
    26941 tggtgatatt gagcttatcg gcaatcctaa attggttgcc ggagcaacga ttttattgcg
    27001 taactttggc gtgtttagtg gcaagtatct gattaaatca tcacgccata cgattagccg
    27061 tagcggtggt tatactactc atattgaggt cagaatgttg gagtttattg ccgatgattt
    27121 aatcacatta ggtatggagg ctggcaatgc aaacgcataa ttttggggcg acttatcaag
    27181 aagggattgt atccgctgtt gatccgaaaa cgcacaaagt gcggtgtaaa atccctgcgc
    27241 tggaagattt agaaactgct tggctgtcgt ttttgacccc gaacgccggt ggcaatcagt
    27301 tttactgttt gcccgatgtg ggcgagctgg tggcaatgct acttgatgca caaggcgaag
    27361 gtggctgtgt gtttggggca atttataacg agcaagaccc gacaccggtt gccgatagcg
    27421 atatgtgggt taagaaattc agcaatggca cggagatttc gcataatcgc aaaaccggcg
    27481 atgtggtagt gaagactaac gggcatttaa ccgcaacggc tcaaggtggg gctacgatta
    27541 atgcaaacac cgtagtcaat ggcacattgc acgctaccgg taaaatcacc tctgacgaag
    27601 aagtctctgc tcctgttgtg aaacaaggca cggtagagct tggcacacat aaacacggcg
    27661 gtagccctga acctaataag taattcttta aaccagttta aaatcccttt taaagatagc
    27721 cctgtatcat cagggctatg aacagacaaa cttccattca attaacccac tggcaacttg
    27781 caccctcaac cgatacagtt gaggtggtgc aaggtattga tgatattcat ctttgtattg
    27841 tcaatatcct ttcaacccaa aaaggcacgg atattttacg ccctgaattt ggctctgacc
    27901 actttcgcta cattgactac cctgaagatg tggctatccc gaattttgta cgggaaatta
    27961 caaccgcctt gcaaaaatgg gaaaaacgca ttgaggttga agaagtattg gtggacggtt
    28021 cagctccgca ttttattttt actgtgatgt ggcacttaac cgatgatgtt tatcgtgaaa
    28081 tttaccgaac aacggtataa ggataggcaa tgacagaagc gattaaaatc attgatgacg
    28141 atgtaaaaac cgtacttgcc gaagcgattg ccgattatga aaaacgcacc ggtaaaatct
    28201 tacaacctgc acatattgag cgttccatta ttcaaaccta cgcttaccgt gagcagttat
    28261 tacgcaaagg cattaatcac gcctttttac aaacctttcc gcagtttgcg acggggattg
    28321 cgttggattt gtgcggggaa acttttggtt gttatcggct tagagataaa cctgcccgta
    28381 ctttactacg cttttcagta agtggtgaac atacagctat agttatccct aaaggaacgc
    28441 aggtagcaat taatgacgag ctatactttt caacgctcaa tgacgatgtt attactccat
    28501 taattaccta tgtggaaatt gatgcagagt gtaatcaggt tggtgttatc ggtaatggtt
    28561 gggaatcagg aagagtcagt aaactaaaaa cagcactggc aactaattat aaagtaacag
    28621 ttgaaaacat tgatattacg agcggtgggt tgatgcagga agaagatgaa ccttaccgta
    28681 agcgaatttt agccgcaccg gaagccttta gtaactgtgg ctctatcgct gcatatgatt
    28741 accacgtccg ctctgtatcc caagatattg cagatgtgaa tatctctacg cctaggggtg
    28801 gcttggtaag aattgcggta ttgacccaaa aagggttacc ggatacccga ttacttgatg
    28861 atattaaaaa acacgttagt gccgaacggc tacgcccact ttgcgatatg gtcgaggtta
    28921 ttaagcccac agaacgccgt tatcaaatta cggcacgttt aatattgtta gatggctacc
    28981 gtgaggattt ggtaaaagca aaagcccgag aggcattaca actctatttg tcagacaaga
    29041 ctaaaaaact cggtttagac attgtgccgt cggccatcat ttcagcttta agagttgagg
    29101 gtgtttacga tgtgaattta attcagccag tcaaaactat tatcaacgaa acagaatggg
    29161 cgaactgcac agcaatcagc gtagaagtcg aagaggagcg tagagatggc tagcttgcac
    29221 tatgcggaca ttattgtgcg agaccctaaa tataaagcat tagcggattt aagcaagcgg
    29281 ttaaatttgc tcgatacttc gcaaattatg actacgctgg tggatttatt gggcgacgaa
    29341 tgtatcccat tgctggcaga aaaatggtcg gtaaccggct atgacggctt attggttgca
    29401 gatagcgaaa actcaaagcg agccttaatt aaaagagcaa tcgaactaca tcaactaaaa
    29461 ggcacgcctt ggtcaattcg tgaagtatta cgcagactag gtttcggaga ggttgagatt
    29521 gacgagggac tgaaaaatcg gctgtatgaa agcacttatg tgagcggaat ccccgaaaac
    29581 gaacgctggg cgcactatgc gattaggttg agtaacccga ttaccaatga gcaagctcgc
    29641 aatatccgta aggtcttacg caattttgct ccggctcgtt gctctcttgc agttttggat
    29701 tataaagcag atccaattcg atataacaat aaagcaacct acaacggcac atataaccac
    29761 ggttcaagtt aaggataaat tatggcaggt gtacaagaaa ccgcacagtg ggaaaaccac
    29821 gtttacagaa ttgaagaaaa tgacccagtc cacggaggag aaaacggtgt aaccaataag
    29881 ccaattaagc acctagcaaa ccgcacgctc tatctgcgta gattgctcac cgaggcagga
    29941 caacgcatta acccgaaaaa aatcacagct accagcaaaa atagtaatga tttaactggg
    30001 catactcacg agattgacca cgcaagccta acgcaaaaag gcattgtgca gttaaatgcg
    30061 agtatccaat caactagcac aactgagtct gcaacccctt cagcagttaa agcagcctat
    30121 gataaagcag caacagcaga acaaaaagcg acattggcaa atgagaaagt cgaggcgatt
    30181 aaagtaggcg gacgaaacct cctcattcaa tccaacactc cacacaacgg gaatgcgtac
    30241 tcagtcagat acgcattggc tgaggctccg aaagtaggcg aagagattgt agtcactcta
    30301 tggggtgaac tcggctctca acgtactgga attggcgttt acaactctca aggtgctacg
    30361 gagttggcaa aactggtcaa aattcgtgat ggagtttatc aaggtaaagg catttggcgt
    30421 aagccgatga gtgagggacg agagcgaacg cctaacgaca cgcacctgaa tgtgtatttt
    30481 tacccgaaaa ccgcaacaga taataaccgt attgagcgaa tccagcttga gcgaggctca
    30541 atcggtacag attggacgcc aaacgaggca gacttaaagg cagagattag tgcaatccgc
    30601 actaaactaa tccctgtacg gggtggcaat gttgctacaa ccttaaatgc ggctaagcgg
    30661 tcggattggt ttagagaaat gcaaatccct tatcaaagtg gttgggggta ttacacccaa
    30721 gcatttcata actcagggac gcaacacaac tacagcaatt taccgctgac aggtcgttat
    30781 tattttatcg ccgaactcta tcataactct ggttattctc acttgcgtat cacctatcca
    30841 agtttaaaac gcacctttga gagtgctatc aaccttaaca atgaggattt gattctagat
    30901 tggcgtgagg tggtgttttt aaaagatgga ttgtatcaag gcacttttaa aacaacaggc
    30961 acactagagt ctgcttatcg tctaagagcg gctcgtgatg atgaacgtta tcagccatac
    31021 ctgcaaatgg tcgatatggg ctatgacctt gccaatcccc cggtaggaaa aaacaaaatt
    31081 attggcagtg ttgattatta cgttaaaaac ggcacaagcg agtcttccaa agcgacgatg
    31141 atggcagcgg caatgcctga caagaacgta ttttgggaag tagcattgtg gaatgcagca
    31201 cagcaacgga atgtattgtt taaggggtat agcaagacta acaatttgag tattggtaaa
    31261 accaccgaca atgagcgaga ccgagtacaa gtcaatggca caatccaagc caccgcaccg
    31321 gctgataacg ccaataacga ccaagtgccg actacggctt gggtgcgtaa gatggcaaag
    31381 attttagctg atggcaaggt gagtaaagcc ggtgacacaa tgactgggca tttattcatc
    31441 aagaatgggg aatactctgc ggtacatacc tataacacaa gtggttggtt cgcaaaatgg
    31501 gaggcggccc cattatctga ttcgcacttt gctgccattg tatatgccaa cgacagagag
    31561 acagttatca atcgggtgtt aattcccaag aagaatggca cagtcgcttt aattaatgat
    31621 attgccgata ccgttagagc cgctgtgagt cgtagtgata aatcccgtag tagaactttt
    31681 cctctgcaaa cggggaaaaa atatacaacc acagggagcg ttacgattta tccagatggc
    31741 cgaattgtgc agattttcca cttaaaaaat attaaagcgg cgtggtttga ttatgaggca
    31801 agtgcggtag gagataggca tagagttgtc aatatccctc tttggactgc gatgccaaat
    31861 aaaatttttg atgtcgatgt taaaactgtt cgcccctcaa ataatgcctc aacttactac
    31921 atagaagcgg ctgaatggct gagtgcgtgg cagatttacg gtaatgacac gaacaaatca
    31981 agtgtcaata ttaatctatc tcgattcaga ggtggaaagg atgaagatat ggacttgtat
    32041 gtaacagttg agggatatta aatggcatat tttgtgaatg tgattgatga gcaaggcaat
    32101 tatgagctaa ttgatgatga gtttattggg ctgtatccgg atttggattt taacacatta
    32161 cccaaactca cggataagca atatcagacc taccttgcca aagcaaacgg caaagagcga
    32221 tttgtaaatg gcgaatttgt gtatgagcaa attaaggtgg atatgcaagc ggtcatttcc
    32281 gctgaaaaat ctgcaaagtt agcagagatt aaccaaaaag cacaagcctt tatcaatgat
    32341 ttagccaaat acaacgaaac gcctaagttt gagcgtgata cttggcttga gcaagcgaaa
    32401 gaggcaaaag cgtgggtggc tgacccaacc gtacaaacgc caacccttga gttaattgcg
    32461 caaatgcgag gcataccgct cgacacgcta cgccaaaaag cctacgaaaa agcgatggcg
    32521 tatcaaacgg tggctgctat tgtagcaggg caacggcaag gatacgaaga ccgcctagaa
    32581 caagcggaaa ccttagagca aatacaggcg attgagccga tgtatcaatt accgcaagga
    32641 gcaaacaatg agcaaactga ctaaattaaa atcgtggagc taccacgtct taattgcaat
    32701 agatcaactc tttaacgctc tcaccggtgg agcagcggac gaaacatttt ctagccgttg
    32761 ctaccgtggt gcagtgcttg cggaaaatcc gaaaaaacgt tggcgttttt ggcactcttt
    32821 tttgaattgc ttatttttag acccaaagca ctgctacgcc gcttataaag cggaggtaca
    32881 ccgcaagcag tatccgaagg catttagact tggggggatt taatgaaaaa gcaaaactta
    32941 aaattagtgc ggggcgatga tgtgagtttt agattcagca ttaaagacag caccggacaa
    33001 cctcttgcgt taaaggattt gtggtttgat ttgcacttag tcagctatca aggtgaggtg
    33061 ctgattgctc tctctactga tgatggcagt attgagacag cagaggctgg tcagatggtg
    33121 ttacatttta gtcaccagca aactgctcga gctgaatgga caagtgccaa ttatgacttg
    33181 caactcacgc acgactatac aagagtctag gaggtggaga tgacctctat cattgaacta
    33241 gacctgactg atgacctcat cattgaggtg cagttagata gcacaatcat taccggcgat
    33301 agctttttag ccaacctgcc ggatttactc caaatttaca accaagcaaa gtcagatagc
    33361 agtaaggagc ttaacaatga ctaaacacaa aaaccacgaa aaccacccga ttgctataac
    33421 attctaccaa cttggcaaag atgtcggcga cattaaccgc cggctagcgg ttattgagca
    33481 acgtgagcaa tcagcacagc caagccaacc acaagctgag gcggttacaa tgccacagca
    33541 agatgctgtt gccattaata acatcaatgc cgttttggag gcggtcgaaa aatacaaaac
    33601 ttttgaaaaa tacaacactc agtggcaaga gcgttttgaa aaattcctgt ctcgtaaccc
    33661 gaatgattac gagttaagtg aagagcaacg ccaaaaactt taccacacga tgtgccagca
    33721 tttgtggcag gtgaacttta ctgaaatcaa tagtaaacag tatgaattac cgatcccgca
    33781 acaattccag ggtaagtttg gctatattgt caaaacctcg ggcggtagca cagaaattag
    33841 ttttgagttg ctatctgaaa ctcacagcac ttatctttat ccggatagct atattcccga
    33901 gggcggtggc gatgtaatgc tcaagccggc ttatatttat ttagtgattc cggacttagt
    33961 ggcaatgcgt gagccggagg tggtgggggt ttcaccgctg aaactgaaca ttctgcagaa
    34021 acacctgctg acacctcaga aactacagga gcagaagccc ttgaacacga aggcaaactg
    34081 attgatggcg tatggcattt tgagcgagag ccggtaaatc caccgagaga tcagaattat
    34141 tatgtggcag gtaacctgtt ctacaacggc ataccggatt gataaattga tgtttcagta
    34201 aacctaatta ggggggaatc cccctttttt attattataa ggaaagaaaa tgaataaatc
    34261 tttagtagcg ttagctgttg cagcgatggc atcgaaagca caaactaaa
//
LOCUS       DANLXF010000002        30584 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.002, whole genome shotgun sequence.
ACCESSION   DANLXF010000002 DANLXF010000000
VERSION     DANLXF010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 30584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30584
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.002"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            3..503
                     /locus_tag="PXD66_000051"
     CDS             3..503
                     /locus_tag="PXD66_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013745376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDL6318395.1"
                     /translation="MSNIGLDKEIAKKLADELNGLLATYQVFYTNVRGYHWNVRGVNF
                     FELHAKFEEIYDDLVTKVDEIAERILTLGHTPQNAFSQYLTKSQIQEHIGVSAAQECL
                     RGTLNGFKTLLGQQREILALANDANDEGTASQMSDYIKEQEKLVWMFTAACAECSDYA
                     QGCSCN"
     gene            562..1275
                     /locus_tag="PXD66_000052"
     CDS             562..1275
                     /locus_tag="PXD66_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HDL6318396.1"
                     /translation="MAMSQGFQFKQFFIRHDRCAMKVNTDGILLGAIAHIKDCQNILD
                     LGIGSGLVALMLAQRTDDCCHITALELEPNAYQQAVENAQNSAWANRISVLQGDVMQQ
                     AFEQKFDLIVSNPPYFADSLAARTTERDLARSISHYSHLNWLAKAKPWLAENGKITLI
                     LPIAAAEKLVDQSEQIGLYCVEQWLIFTKSGKLAKRSIVSFSLNKQKREHKELIIYDL
                     DNCYTTEFKRLTQDFYLNF"
     gene            complement(1386..2390)
                     /locus_tag="PXD66_000053"
     CDS             complement(1386..2390)
                     /locus_tag="PXD66_000053"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005651072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDL6318397.1"
                     /translation="MAFNLKNRHLLSLVNHTEREIRFLLDLARDLKRAKYAGTEQQKL
                     KGKNIALIFEKTSTRTRCAFEVAAYDQGAHVTYIDPNSSQIGHKESMKDTARVLGRMY
                     DAIQYRGFKQSVVQELADYAGVPVFNGLTDEFHPTQMLADVLTMIENCEKPLSQISYV
                     YIGDARNNMGNSLLLIGAKLGMDVRICAPKALLPEDAFVKMCEKFAAESGARMTVTED
                     IETAIKGVDFVHTDVWVSMGEPLESWTERIDLLMPYRVTPELMQATDNPKVKFMHCLP
                     AFHNSETKVGKEIAEKYPALANGIEVTEEVFESPMNVAFEQAENRMHTIKAVMVASLA
                     "
     gene            complement(2478..3317)
                     /locus_tag="PXD66_000054"
     CDS             complement(2478..3317)
                     /locus_tag="PXD66_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDL6318398.1"
                     /translation="MQSYPYLLVNVFATSHFGGNPLAVFYEADGLTDLQMQLIARQFN
                     LSEVVFIQSSTDSQAVKKLKIFTPDYEMPFAGHPTVGAAFVLHSAFDFPTTYRLQTNA
                     GLVAIQHQHNVTTFALTNGVNLENTPLSRAECASILGLNVADIASEPCYVNTGVEQLL
                     VELASQQAVEKCKINANLAEKAFLKDSVYVWCREGNEAKVRLFFQSQGSIVEDPGTGS
                     AAANLGGWHIAQGKIPIQLQISQGDEIQRPNRLLLKVDQNGKIFVGGEVILVGKGEFY
                     LPE"
     gene            3507..4841
                     /locus_tag="PXD66_000055"
     CDS             3507..4841
                     /locus_tag="PXD66_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HDL6318399.1"
                     /translation="MLYAEFLLLLAFLYAGSRYGGIGLGVVSGIGLFVEVFFLGMPLS
                     APPISVMLVILAVVTCASILEAAGGLKFMLQVAERILRSNPKRITFLGPLVTYLMTLM
                     LGTGHSVYSIMPIIGDIALKNKIRPERPMAAASVASQLGITGSPLSAAVAYYLTEIVK
                     LPGFEGVTLLNVVGVTITATFCGVIAMSLYSLRRGKELEDDPEYQRRMQDPELRKQIE
                     QTSATSLDEKLPASAKNSVYLFLAAIATIVIIAMIPAIKPTVAATGKVAGMDQIIQIV
                     MLTFGGLILILTKTDPKKVPNGIVFKSGMVATIAIFGIAWMSDTYFTHAMPAFKEGVT
                     EMVRAQPWTFAFALFAVSVVVNSQAVTAKMLLPVGIAMGLPAPLLVGLMPATYAYFFI
                     PNYPSDIATVNFDVTGTTKIGKYYFNHSFMAPGLVGVITACIVGIAVANVLI"
     gene            complement(4921..5331)
                     /locus_tag="PXD66_000056"
     CDS             complement(4921..5331)
                     /locus_tag="PXD66_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDL6318400.1"
                     /translation="MNSTLTNLQPLAFLITRVVAGYMFLLHGTAKFFEFPMSMTNGNG
                     SVPLASIYGVGGILEIVGGIFFILGLFTRQTAFILSGMMAYAFFIAHGAQGNVLLPLL
                     NGGELAGVYSVLFLTYIFIGGGKYSLDAKLFNKA"
     gene            complement(5454..6131)
                     /locus_tag="PXD66_000057"
     CDS             complement(5454..6131)
                     /locus_tag="PXD66_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDL6318401.1"
                     /translation="MNLFGFRCFHCNHPLAIGVQGICSHCQKLLKPTPYCRHCGMSAV
                     EFRNACGYCLKNEPKWHKLVRVNEYRAPLSHWVHQFKFQQQYWWDQALARQLLLAIKQ
                     AQREQVLSLPEVIMPVPLFWHRHWKRGFNQSELLCDWLSKWLQIPLDTQSLQRVRATV
                     SQRELTAAERRKNLRGAFFYQPLKAYKRVAIVDDVLTTGSTLNVICAELLKAGVVEIQ
                     VWVLARA"
     gene            6249..7061
                     /locus_tag="PXD66_000058"
     CDS             6249..7061
                     /locus_tag="PXD66_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL6318402.1"
                     /translation="MKSLQKPIKIVFFDIDDTLYVKHKAYIPKSITEQVLPRLKAKGI
                     IPAIATGRNHGCFPEALKPYLNPEIGFELFVTINGQYNFYKDQIISDYSLSREQIERI
                     IEKANALGILYAFITPMTIAVSETNPIVHEAVLPITPNYLIDPHYYEKEKIVQMLLFY
                     TEEQKQAVENSGIFANDLKEARWHPNAVDILRKENSKARGIQDVLATLNIEIESAMAF
                     GDGINDLEMLPTVGVGVAMGNGVPELKAVADFITKPLEEDGILYALETLEVI"
     gene            complement(7109..7378)
                     /locus_tag="PXD66_000059"
     CDS             complement(7109..7378)
                     /locus_tag="PXD66_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDL6318403.1"
                     /translation="MGLLYLLLLNTLIGYGSYGEALNHWDTSKVSIVTTMLPIFTMIF
                     SNLSYYFYPHIFAKPDMNWISYLGALVVVSGAILAIAGDKLFRRN"
     gene            complement(7351..8028)
                     /locus_tag="PXD66_000060"
     CDS             complement(7351..8028)
                     /locus_tag="PXD66_000060"
                     /inference="COORDINATES: protein motif:HMM:NF013088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL6318404.1"
                     /translation="MKQRPLLGFSLALTAAMTWGTLPIAAQQVLKVIDAQTLVWVRFV
                     VASVGLLLILGFAKRLPKLTACSKKELGLILLGVCGLSANFFLFAEALHYVSPTINQV
                     LWQLAPFSMILLGVLLFKEKFGLFQKIGFVLLIAGLVAFFNDKFAEILQLNTYALGVL
                     LSAGASLIWVLYGVAQKLLLAKFNSQQVLFIIYIGCSLLMLPFASPAQLGNLEDGFLW
                     GCFIYCC"
     gene            complement(8130..8387)
                     /gene="rpmA"
                     /locus_tag="PXD66_000061"
     CDS             complement(8130..8387)
                     /gene="rpmA"
                     /locus_tag="PXD66_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDL6318405.1"
                     /translation="MATKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGSNVGMGKDHTLFATADGKVKFEVKGEKSRKYVSIIAD"
     gene            complement(8408..8719)
                     /gene="rplU"
                     /locus_tag="PXD66_000062"
     CDS             complement(8408..8719)
                     /gene="rplU"
                     /locus_tag="PXD66_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDL6318406.1"
                     /translation="MYAVFQSGGKQHRVSEGQVVRLEKLELATGEKVEFDSVLMVVNG
                     EDVKIGAPVVAGAKVVAEVVAQGRGEKVKIVKFRRRKHSRKQQGHRQWFTEVKITGIQ
                     A"
     gene            8997..9995
                     /gene="ispB"
                     /locus_tag="PXD66_000063"
     CDS             8997..9995
                     /gene="ispB"
                     /locus_tag="PXD66_000063"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HDL6318407.1"
                     /translation="MAVQLFSLEQIQALANEDMQAVNAEILAQLNSEVLLINQLGHYI
                     IAGGGKRIRPLIAVLAARALGYRGKKHITAAAFIEFVHTATLLHDDVVDESDMRRGRP
                     TANAHFGNAASVLVGDFIYTRSFQMMTDLDSLRVLKVMSAATNALAEGEVQQLMNVND
                     PNTTEANYMRVIYNKTARLFEAATQCVAIAADASEEIETALKNYGCYLGTAFQLIDDI
                     LDYSAQSESLGKNVGDDFVEGKPTLPLLHAMHAADKAGKTTQAELIRNAIENGGELEQ
                     IPQVLAIMQEHQSLDYAMQKAREEAQKAVDSLGVLPESEYKQALVSLAYLSVDRSY"
     gene            10098..10844
                     /locus_tag="PXD66_000064"
     CDS             10098..10844
                     /locus_tag="PXD66_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HDL6318408.1"
                     /translation="MTEQNQRPQEHIESVRARRRAQNQKKGPNAPFVREKLTLPNGHN
                     KLLLHSCCAPCSGEVMEAIHASGIEFTIYFYNPNIHPLKEYLIRKEENIRFAEKWGIP
                     FIDLDDDYENDRKEWFKKAKGMEWEPERGIRCTMCFDMRFEKAAEYAHNNGFPVFTSC
                     LGISRWKDMNQINDCGHRAAAKFDDVVYWDYNWRKGGGSQRMIEISKRERFYQQEYCG
                     CVYSLRDSNKWRLDTGRQRIEIGKLYYSPD"
     gene            11261..12196
                     /gene="fabD"
                     /locus_tag="PXD66_000065"
     CDS             11261..12196
                     /gene="fabD"
                     /locus_tag="PXD66_000065"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDL6318409.1"
                     /translation="MSKFAMVFPGQGSQAVGMLADLAPQYPIVEETFKQASDVLGYDL
                     WDLVQNGTAEELGQTHRTQPALLASSVALFRIWQQKFPEQKPAVMAGHSLGEYSALVC
                     AGVLDFQDAIKLVELRGRAMQEAVPAGSGAMFAIIGLDNESIIKACEQAASETGEIVS
                     AVNFNSPGQVVIAGTKAAAEKAGELCKAAGAKRALPLAVSVPSHCALMKPAAEKLAEA
                     LQNIELNQPLVPVINNVDVAVETDADTIRNALVRQLYSPVRWTETVEKMAQDGITTLY
                     EIGPNKVLTGLAGRIVKELSAKAVNDVASFEAVEA"
     gene            12213..12677
                     /locus_tag="PXD66_000066"
     CDS             12213..12677
                     /locus_tag="PXD66_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318410.1"
                     /translation="MSKETLFMLISALIALVILPLAYTVGIAKETLGGNMGMLSIAAG
                     CSFLAYFLSLNPKIRHAFIGGSAITAAAIQAWVLYGVLLSENDPKSAVVLFQIYLFSS
                     LGPLFFSAIWAFTRPRWIYPKQGSAVKYALFGGLTTVINYLITLFIFYILTL"
     gene            12698..13420
                     /gene="fabG"
                     /locus_tag="PXD66_000067"
     CDS             12698..13420
                     /gene="fabG"
                     /locus_tag="PXD66_000067"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HDL6318411.1"
                     /translation="MSKIALVTGATRGIGKAIAEELVAKGYIVVGTATSEKGAESISA
                     YLGENGKGLVLNVADAESIESVLKQIKETFGDVDVLVNNAGITRDGLLMRMKDEDWFD
                     IIQTNLTSVYRLSKAVLRPMMKKGGRIITIGSVVGSMGNPGQTNYCAAKAGLVGFSKS
                     LAKEVASRGITVNVVAPGFIATDMTDELTEDQKNAILSQIPAGELGSPQDIAKAVAFL
                     ASDDARYINGETIHVNGGLYMS"
     gene            13539..14837
                     /gene="brnQ"
                     /locus_tag="PXD66_000068"
     CDS             13539..14837
                     /gene="brnQ"
                     /locus_tag="PXD66_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDL6318412.1"
                     /translation="MTRNTLVVGFLLFAIFFGAGNLIFPPKLGYESSTEFFSAISGFV
                     LTGVGLPLLGIIVSAFYNGGYKSALDKIHPWFSLAFLTSIYLAIGPFFAGPRTAATAY
                     EMAIMPFLGDSSQVSMLIFTLIYFAFTLWLALNPNKMVDRIGAILTPILLITIIGLVI
                     RAGFILDTDPQITTPNTNEQSYFFKGMVDGYLTMDALASLAYSVIVLNAIRGKGVSQA
                     ALQRQTVSAGLVAAVALAVIYISLGWIGNNMPISANTLSMLSERKLDLGTYILNTVTT
                     QAFGELGRSVLGVIVSLACLTTAVGLAVSVSEYFNEIFPKISYKVYAILFTVISFTVA
                     NQGLKEVISKSIPVLLVLYPIAMTVILMLLINLVIKLPLSAQRLSLLLVTIVAIASVA
                     GVGFTENLPLKSYSMEWLPFAIIGAIIGAIIHPLTGRKHK"
     gene            14883..15707
                     /gene="mutM"
                     /locus_tag="PXD66_000069"
     CDS             14883..15707
                     /gene="mutM"
                     /locus_tag="PXD66_000069"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HDL6318413.1"
                     /translation="MPELPEVETSVRGVSPYLVGQTIEQIIVRQPKLRWTVSPELSQM
                     AGAKITAVERRAKYLIIRTDKGDILVHLGMSGSLGILQERQQKEIGKHDHVDLITQNG
                     TILRYNDPRKFGCWLWAEKAEDHELLRKLGPEPLSNDFTADYLFAKSRKKTTACKTFI
                     MTNEVVVGVGNIYACESLFMAGIHPELAAQNLTKKQCERLVKTIKEVLTKAIIQGGTT
                     LKDFIQPDGKPGYFAQVLQVYGRKGETCNDCGKPIETKVIGQRNTYFCPKCQRLPK"
     gene            complement(15790..16224)
                     /gene="dtd"
                     /locus_tag="PXD66_000070"
     CDS             complement(15790..16224)
                     /gene="dtd"
                     /locus_tag="PXD66_000070"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDL6318414.1"
                     /translation="MIGLIQRVKFAKVEVEGQVVGEIASGLLVLLGVEKDDDEAKADK
                     LLEKVLNYRVFSDSEGKMNLNVQQAGGSLLVVSQFTLAADTNKGLRPSFSKGASPVDA
                     ERLYAYFHQQAATKIHTQTGQFAADMQVSLQNDGPVTFWLQV"
     gene            complement(16199..17044)
                     /locus_tag="PXD66_000071"
     CDS             complement(16199..17044)
                     /locus_tag="PXD66_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HDL6318415.1"
                     /translation="MLNRLWRFIKLFAKRYNENNIAISAGYLTYSTMLAIVPFIVVLF
                     SIFTFFPIFHEANEQLKAFIYDNFAPSASEMVSEYIELFVSNAKQMGLVSFIGLFVVT
                     IMLIKSIDEALNTIWHDNRQRPMWFSFLLYFLILLFGPLLAGASIAISTYVMSLSIFN
                     GNGALSLGQHLLELMPFLLIWLLFTLVYKFIPIRKVKFQHAAIGALMAAIFFTLGKKA
                     FIWYITAFPSYQAIYGALAVLPIMLLWIQLSWQVVLLGGQFAAVLDEMETDKGSINDR
                     LNSAG"
     gene            complement(17046..18284)
                     /gene="proA"
                     /locus_tag="PXD66_000072"
     CDS             complement(17046..18284)
                     /gene="proA"
                     /locus_tag="PXD66_000072"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDL6318416.1"
                     /translation="MQMIELAQQAKQASFELAQFGNLQKNQALLTIADSLEQRAEEIL
                     YANAKDIEFAQAQGISLAIIDRLLLNEDRLKDIANDVRNVAKLADPVGQVMDGGVLDS
                     GLKIERQRVPLGVILTIYEARPNVTIDVASLCLKTGNAVILRGGKETKFTNAVLVDVV
                     QDALEKAGLPKLAVQAVTDPDRALLLELLKLDRYIDMVIPRGGAGLHQFCKENSTIPV
                     IVGGIGVCHLFVEKTADQEKSLEVIANAKTQRPSTCNTLETLLVEKAIAVEFLPKLVA
                     KMQQLGVTIHSNDLQKIEGIEPLDQAKLSQEWLSLDLNVVVVNDLQQAVEHIRHYGSQ
                     HSEGILTSDYQLARQFVQQVDAAAVYINASTRFTDGGEFGLGAEVAVSTQKLHARGPM
                     GLEALTTYKWVCEGDYLVRG"
     gene            18568..20451
                     /gene="mutL"
                     /locus_tag="PXD66_000073"
     CDS             18568..20451
                     /gene="mutL"
                     /locus_tag="PXD66_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDL6318417.1"
                     /translation="MINTTPKQLIHILPPQLANQIAAGEVVERPASVVKELVENSLDA
                     GANTIQIDIEKGGSQLIRIRDNGCGISKQDLPLALARHATSKISSLEDLEAILSLGFR
                     GEALASISSVSRLTLTSRTAEQTEAWQAYAQGREMAVEIQPASHPIGTTIEVANLFFN
                     TPARRKFLRTDKTEFAHIDEVVRRIALAKPNISFSLSHNGKIVRQYRKVQDNSVEQQQ
                     KRVAAICGDEFIQHSTYIDRQHGDLHLHGWVGSPALARLQNDLCYSYVNGRMMRDKTI
                     NHAIRQAYGDSIASGYYPAFVIFLDLDPTMVDVNVHPAKHEVRFHQGRLVHDFILQGV
                     LNSLQQTQPTLELRNEVNEPLPSYVQDTNRQAAGQNIFVSQTAPQAAEFTQKNASRSS
                     YTPKFERSNSVSSSAQKWYKELVGGDLHQTTPTIKTQSTLVEPKTEPQTEHKTEAVSQ
                     KITQIAPLASHHSQALAIVKNQAVLIKEEENFYLLPLATLAELKLKLQLEKGQSQALL
                     IPLNLSLDQQQTERWQQTKEELTKLGFSISEKQWQAQTRLTVLSVPQAVREQNLQQLL
                     FALFNQTQAVNLAEFFAKKCEKPTACTLSDVIALLSEIELYANGKKELEGIRKEVDFS
                     KFL"
     gene            complement(20470..20739)
                     /locus_tag="PXD66_000074"
     CDS             complement(20470..20739)
                     /locus_tag="PXD66_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HDL6318418.1"
                     /translation="MREIEYSNAFKRDYKKCAVESLSSSLIEALYFLINDKPLPEKYC
                     DHALTGEWKGFRDCHIQPDLVLIYRKVEDDKLELLRIGSHSHLFG"
     gene            complement(20726..20992)
                     /locus_tag="PXD66_000075"
     CDS             complement(20726..20992)
                     /locus_tag="PXD66_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDL6318419.1"
                     /translation="MANSALVSVRISPEIKEQASEVLAGIGLTVSDVMRMTLAKIATE
                     KRFQFDYQPNFETAEVLRAVKNGEEKLHSANSIADLMEQLNARD"
     gene            21432..22385
                     /gene="miaA"
                     /locus_tag="PXD66_000076"
     CDS             21432..22385
                     /gene="miaA"
                     /locus_tag="PXD66_000076"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDL6318420.1"
                     /translation="MNKKPLALFLMGPTASGKTDLAIALRQELPVEVISVDSALIYKG
                     MDIGTAKPSKEELALAPHRLIDILDPAESYSAMNFHADALREMADITSQGKIPLLVGG
                     TMLYYKALLEGLSPLPSADPAIRAEIESRAAQHGWATLHQELVKIDPIAGARINPNDS
                     QRINRALEVFYITGKTMTELTAQQGEALPYNILQFAIAPQDRAVLHQRIEQRFHKMIE
                     LGFEEEVKRLFLRKDLHINLPSIRCVGYRQMWEYLQGDISLDEAIFKGICATRQLAKR
                     QITWLRGWQGELTWLDSLAPTSSKAKMVEKIDHYLKNCDNI"
     gene            22454..22726
                     /gene="hfq"
                     /locus_tag="PXD66_000077"
     CDS             22454..22726
                     /gene="hfq"
                     /locus_tag="PXD66_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005712219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDL6318421.1"
                     /translation="MAKGQSLQDPYLNALRRERIPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPARALSHNNNNQAQHAQPAAEVTEQAE"
     gene            22746..24131
                     /gene="hflX"
                     /locus_tag="PXD66_000078"
     CDS             22746..24131
                     /gene="hflX"
                     /locus_tag="PXD66_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDL6318422.1"
                     /translation="MLNESLPNSEENSAFGFALSEASQTTEAASPTDPENQDKAILVH
                     LYLGQHKDVENLLEFKTLAESAGVEVLATLTTSRQSSHIKYYVGQGKAEGIAEAVERL
                     GATVVLVNHQLSPAQTRNLQSLCGCRVVDRTGLILDIFAQRARSHEGKLQVELAQLRH
                     LSTRLVRRVTNQDQQKGGAVGLRGPGETQLETDRRLIKVRIQQLLNRLEKVSKQRNQN
                     RKTRQKADIPTVSLVGYTNAGKSTLFNAITQAGVYAADQLFATLDPTLRRIQVQDVGT
                     TILADTVGFIRFLPHDLVSAFKSTLQETTEATLLLHVIDGSDERKNENIDAVNQVLDE
                     IGALDIPTLLVFNKIDKLEGVEPHIERNADGTPVAVYLSAQENSGIELLFQAIQERLR
                     SNLVCENLLLPVTAGAIYAQFKAENCIKTEHFNEFGERIISIEINEVQWNKWVKQFPD
                     LAEYIELAKWG"
     gene            complement(24353..30424)
                     /locus_tag="PXD66_000079"
     CDS             complement(24353..30424)
                     /locus_tag="PXD66_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HDL6318423.1"
                     /translation="MNKIFKVIFNQTTQKMEVVSELARSQGKAASSTDKRGTIKLAAA
                     SILGGATLISGMAAFMLSATPAEAAVIIDRIEVQQKAPKKVVNGGIDIYTNTSLATDS
                     ITNPYNYFNPGSLSYADRTKLQESNNSVNKEKDSRLLYKSNTTSAIVIGHNAVALDNN
                     RKSRGKPKASGIAIGDYSYSGGGLSIAIGSFSRTASIGSVALGTSARAEGFNSFAAMR
                     QSAAIADYSTAIGSTSWANATASFAIGASATALGDRSFAIGSGTPQTVDGSYGHENRR
                     SIYDGQNNTLAFGTDAFSIGTKAKTNGNHSFAIGTNAWSGGFKLENSNISELGQVINP
                     DTAKVANNAFALGTNTRANSNDTLAFSTNASATGDGSIAFGVNSNTTGIGAIAFGNAS
                     KAWKSNAISIGWNANSHAEKVISIGSHSNASKDGAMAIGDTANSSGNNSVAIGYSANV
                     SKANATAVGTRANITHEGGTAIGFEAKSKGNYATAIAYNSTAKGKNSIAAGVNSTVSG
                     IRAAALGYNNTVSGKDTFVLGSNVTVKTKGSVILGSDSTEATATKEDTATVGTLKYSS
                     FAGDENVVDGDYVSVGNATNARQIKFVAPGKIASNSTDAINGSQLYAVADTLVKTGFN
                     ITADHSGLGGGAKEDNVKLGETVKYTSTDKNVVTTVTNNTIDFGLSDNISVGGKPGKD
                     GSVGVKGADGKDGVTIKPEAIVFHGVDGVNGKDGKDGKASIKVEKGAKGLDGNDGKDG
                     ESKTRIVYEKPNGDKEEVATLNDGLKFGANDGKVHDAKLNTQVDIKGATTNTDWAKFD
                     AGKNIMTQVVGNTITVALAKDLAGLDSATFGTQGDSTVINKDGVTIAKKDPNDATKSN
                     VTLTEDGLSNGGNQITNVDSGLKGKDGQPVKLTEAAGDVLNNAVNVGDLKNTVTETVT
                     EVTTDAFGLKDENNKEFKQNLGTTAKIIGDGNVKTNVVEKDGGHALEVSLVNNITVGK
                     DGEPGKVGIAGKDGKDAIALDGKDGAITVGRPGKDGIDGTIGVNGKDGSAVVLNGKDG
                     SIGLNGTNGKDGLTIKGTEGKPGLVGKDGLPGKDGVTRIQYQPTDGNGDPIGEPEQVA
                     TLNDGLHFKGDDATLVSKKLNETLEIIGGADKDKLTANNIGVNSKDGKLSVQLAKDLK
                     DLNSATFKPQDGENGQEGKGPTTVVNNNGVTITPKDGTDGKAVKLTEDGLDNGGNQIV
                     NVKSGIDGLAGKDGINGKDGKPITSVNDLTDDQLKEVGTNAANINDVVNASNDIIEKG
                     FSIAADNGETDHVKLGETVKYTSKDKNIITTVSDNVIDFTLNNDFTIGGPGKDGSIGV
                     KGADGTVGVTLNGKDGSIGLTGPKGADGKDGASATISVKDGAKGLDGNDGKDGESKTR
                     IVYEKPDGTTEEVATLNDGLRFVGDDGKEIVKKLNETLKISGGDTDLTNLSDNNIGVV
                     NNNGALTVKLAKDIDLTKDGSVTIGDTTVNNDGLTIKDGPSVTKAGINAGDKKIINVQ
                     DGEISATSKDAVNGSQLYQVQEVANAGWNLTANGKDKGNVKPGATVDLNNKDGNIIIT
                     KEGNNVTFGLKNNLTIGGKDGKDGQIGIAGKDGKDGVTIKGDGTITAGRDGKDGVDGS
                     IGATGKDGASVVLNGKDGSIGLTGPKGADGKDGASANIAVKDGAEGVDGTNGKDGLPG
                     ENGKTRIVYETKDKDGKTVTEQVATLNDGLIFSGNNEDVKNRQKLNTEVKVKGEGVDK
                     TASENFKSASGNINVKANGTDTLEVQLAKAIDLTEDGSVTTGDTTVNNNGLTIKDGPS
                     VTKGGIDAKGTKITNVKDGDVTATSKDVVSGSQLYNAMQNTGWYLTVDGNVAETSEKG
                     AQRVNNNGKVAVSGGKNIVVSRKGSTVEVATSSNLEFDSVKVGGTTISSTVAKDGVNE
                     LNIAGSKNAQTRITNVAPGVKGTDAVNVNQLKGAENRLNNRISNVDKDLRAGIAGALA
                     AGNLYHVTQPGKSMVSAGVGAYRGQGAIAVGYSRLSDNGKVGIKFSVNSNSRGDTGAA
                     ASVGYQW"
BASE COUNT         8938 a         6810 c         5987 g         8849 t
ORIGIN      
        1 caatgtcaaa tatcggttta gataaagaaa tcgcaaaaaa attagcagat gagttaaatg
       61 gtttattagc tacttaccaa gtgttttaca ctaatgtacg tggctaccat tggaatgtac
      121 gtggtgtgaa cttctttgaa ttacatgcaa aatttgaaga aatctacgat gatttagtga
      181 caaaagtaga tgaaattgca gaacgtattt taaccttagg tcacacacct caaaatgcgt
      241 tcagccaata tttaaccaag tcacaaattc aagaacatat tggtgtttca gctgcacaag
      301 aatgtttacg tggtacttta aatggtttca aaacattact gggacaacaa cgtgaaattc
      361 ttgctctagc aaatgatgca aacgatgaag gtactgcttc tcaaatgagt gattacatca
      421 aagaacaaga aaaattagta tggatgttca ctgcagcttg tgctgaatgt tcagactacg
      481 cacaaggttg ttcttgtaac taagttgcaa taatataatt aacccccgat aatgaaagat
      541 tatcgggggt tactttttca gatagctatg tcgcaaggat ttcaatttaa acagtttttt
      601 attcggcacg acagatgtgc aatgaaagtc aatacagatg gtattctatt gggagcgatt
      661 gctcacataa aagattgtca gaatatttta gatctgggta taggttctgg tttagtagca
      721 cttatgcttg ctcagagaac agatgattgt tgtcacatta ctgcgttgga acttgaacca
      781 aatgcctacc aacaagcggt cgaaaatgcc caaaattctg catgggcaaa tcggatttcc
      841 gttttgcaag gtgatgtgat gcagcaggca tttgaacaga aatttgactt gattgttagt
      901 aacccacctt attttgcaga ttcacttgca gctcgaacca cagagagaga tttagctcgc
      961 tcaattagcc attacagcca cttgaattgg ctggcaaaag caaaaccatg gttagcagag
     1021 aacgggaaaa ttacactgat tttacctata gcagctgcag aaaaattagt tgaccaaagt
     1081 gaacaaattg gattgtattg tgttgaacaa tggttaattt ttaccaagag cggcaaattg
     1141 gctaaacgca gtatagtgag ttttagttta aacaagcaaa agcgtgaaca taaagagctg
     1201 atcatctatg atctagataa ttgttacact actgagttta aaagacttac ccaagatttt
     1261 tatttgaatt tttaatttac tccccgggtt agtgttttcc acaaatcttc ttcagctacg
     1321 atgatctagc cttaagctaa aaagcagatt accacccaag ataatccgct ttttagactt
     1381 agctattatg ccaaacttgc taccattacc gctttaatag tgtgcattcg attttctgct
     1441 tgctcgaatg ccacattcat tggcgattca aacacctctt cggttacttc aatgccgttt
     1501 gccagtgccg gatatttttc ggcgatctct ttaccgactt tagtttcact gttgtggaac
     1561 gccggtaagc agtgcataaa tttcactttc gggttgtcgg ttgcttgcat taattcaggc
     1621 gttactcggt aaggcattaa taaatctata cgttcagtcc agctctctag cggttcaccc
     1681 attgaaaccc aaacatcagt atgcacaaaa tcgacacctt taattgcagt ctcaatatct
     1741 tcggttaccg tcattcttgc ccccgattca gctgcaaatt tttcacacat tttcacgaat
     1801 gcatcttccg gtaataaggc tttcggggca caaattcgca cgtccatccc caatttcgcc
     1861 ccaatcaaca ggagtgaatt acccatatta ttgcgagcat cgccgatata gacataactg
     1921 atttggctaa gcggtttttc gcaattttcg atcatcgtta gcacgtcagc caacatttgg
     1981 gttgggtgga attcatcggt taagccgtta aacaccggca cgccggcata atcggcaagc
     2041 tcttgtacta ccgattgttt aaagccgcga tattgaatcg catcatacat tctgcctagc
     2101 actcgggcgg tatctttcat tgattcttta tgcccgattt gcgaggagtt cgggtcaata
     2161 taggtaacgt gagccccttg atcgtaagct gctacttcaa aggcacaacg agtacgggtc
     2221 gaggttttct caaaaattaa ggcaatattt ttgcccttta atttttgctg ctcagtgccg
     2281 gcatatttcg cccgttttaa atctcttgct aaatccaaca aaaagcgaat ttcacgctca
     2341 gtgtggttta ctaaactgag taaatgtctg ttttttaagt taaatgccat tttgtttctc
     2401 cctgtaaaca ttaaaattta ttttatgcaa aataattgca taaaaattca cttatagcaa
     2461 acagaatttg cttaccctca ttcgggtaaa taaaactcgc ctttgccgac taaaataacc
     2521 tctccgccaa caaaaatctt cccgttttga tcgactttta ataacaggcg attcggccgt
     2581 tgaatttcat caccttgtga gatctgaagc tggattggta ttttgccttg tgcaatgtgc
     2641 caaccgccta aatttgccgc tgccgaaccg gtgccggggt cttcgacaat cgaaccttgc
     2701 gattgaaaaa acagtcgcac tttcgcctca tttccctcac gacaccaaac atatacactg
     2761 tctttcaaaa atgctttttc agccagattt gcatttattt tgcacttttc gaccgcttgt
     2821 tgagaagcta actcaaccaa taactgctcc acgccggtgt ttacataaca aggctcggac
     2881 gcaatatccg caacattcag ccccaatatt gaggcacatt ctgcacggct tagcggtgtg
     2941 ttttccaggt tcacaccatt ggttaaagca aaagtagtca cattatgttg atgttgaatc
     3001 gctaccaatc cggcattagt ttgcaagcga taggttgttg gaaaatcaaa ggcggaatgc
     3061 aacacaaaag ccgctccaac agttgggtgg ccggcgaacg gcatttcata atcaggcgta
     3121 aaaattttga gttttttgac cgcttgtgaa tcggtggaag attgaataaa taccacttca
     3181 gaaaggttaa attggcgagc aataagttgc atttgtaagt cggttaatcc gtccgcttca
     3241 taaaatactg caagcgggtt gccgccaaaa tggcttgttg caaacacatt caccagcaaa
     3301 taaggatagc tctgcattta tcactcctta cataaactta aagccacatt gggcgaaaat
     3361 tctaccattt tggtggcgaa tcgtaaaatt ggaaaattta tcggtatccc tctttagagt
     3421 taactatttt tgatttatct caaagtttta aaaaaaagat taggtcatac ttctttctag
     3481 tgattatttt ttaatttgag gttcttatgc tttatgcaga atttttactt ttattagcct
     3541 ttttatatgc aggtagccgt tacggtggca tcggtttagg tgtggtttcc ggcatcggct
     3601 tatttgttga agtgttcttc ttaggaatgc cgctttccgc tccgccaatt agcgtgatgc
     3661 tcgtgatttt ggctgtagtt acttgtgcct ccattctaga agccgctggc ggtttgaaat
     3721 ttatgttgca agttgccgaa cgcattttgc gtagcaaccc aaaacgtatt acctttcttg
     3781 gcccacttgt cacttatctg atgaccttaa tgctcggcac agggcattcg gtttactcta
     3841 ttatgccgat tatcggtgat attgccttaa aaaataaaat tcgcccggaa cgcccgatgg
     3901 cagcagcctc tgttgcttca caattaggta ttaccggtag cccactttct gctgcagttg
     3961 cctattactt aaccgaaatc gttaaattac cgggctttga aggggtgact ttattgaatg
     4021 tagtcggcgt aaccatcacc gcaaccttct gtggtgtgat tgcgatgtca ttatatagct
     4081 tacgtcgtgg taaagagttg gaagacgatc ctgagtatca acgccgtatg caagaccctg
     4141 agctacgcaa acaaatcgag caaacgagtg caacgtcttt agacgaaaaa ctaccggcaa
     4201 gtgcgaaaaa ctcggtttat ctgttcctag ccgctattgc aaccattgtg attattgcaa
     4261 tgattccggc tattaaacca acggttgctg ctacaggaaa agttgccggt atggatcaaa
     4321 tcattcaaat cgtgatgcta actttcgggg gtttaatctt aattttaact aaaaccgacc
     4381 cgaaaaaagt accaaacggc atcgttttca aatccggtat ggtagcaacc attgcgattt
     4441 tcgggattgc ttggatgagt gatacttact tcacccatgc aatgccagcg ttcaaagaag
     4501 gggtaacaga aatggttcga gcccaacctt ggacattcgc cttcgccttg tttgcggtat
     4561 ctgtagtagt aaacagccaa gccgtaaccg caaaaatgtt attaccggta ggcattgcaa
     4621 tgggcttgcc tgctccatta ttagttggtt taatgccggc aacttatgct tacttcttta
     4681 ttccgaatta tccgtctgat atcgcaaccg ttaactttga cgtaaccggt acaactaaaa
     4741 tcggtaaata ttacttcaac cacagcttta tggctccggg tttagttggc gtaattactg
     4801 cctgtattgt cggtatcgcc gttgcaaatg tgctaattta attttaacaa aaaaacaagc
     4861 ggtcagatta agtaaatttt ttaccaaatc tgaccgcttg attatttact gcaaaaccaa
     4921 ttaggctttg ttaaagagtt ttgcatctaa cgagtatttt ccgccaccga taaaaatata
     4981 ggttaagaat aataccgaat acacgcctgc taattcaccg ccgtttaata aaggtaatag
     5041 cacattgcct tgagctccgt gagcaataaa gaacgcgtat gccatcatac ctgaaaggat
     5101 aaacgctgtt tggcgggtaa atagacctaa aataaagaaa attccgccca caatttccaa
     5161 aataccgcct acaccataaa tggaggcaag gggtacactg ccgtttccgt tggtcatcga
     5221 cattggaaat tcaaaaaatt tcgctgtgcc gtgtaataaa aacatataac cggcaacaac
     5281 acgggtgatt aaaaacgcta aaggttgtaa atttgttaaa gtagagttca tttgctttcc
     5341 ttaaaataaa ttgtgatata aaggtcaatt gattgaggca ggcattctat tttaaaaacc
     5401 tatacttcac aatccagccg tcatgaaata gattatttac cattaataaa caattaggct
     5461 ctcgctaaca cccaaacctg aatttctact actccagctt tgagtaactc ggcacaaatt
     5521 acatttaagg tggaacctgt ggtgagcaca tcatcaacaa tcgccactcg tttataggct
     5581 tttaacggtt gatagaaaaa tgccccacgc agatttttcc gccgttcagc agcggtcaat
     5641 tctctttgtg agacggttgc tctcacacgt tgtaaactct gggtatccag cggaatctgt
     5701 aaccacttcg ataaccaatc acacaataat tcgctttgat taaaccctct tttccaatgt
     5761 ctgtgccaaa aaagtggtac aggcataatc acttcaggca agctcagaac ctgttccctt
     5821 tgagcttgtt tgattgccag cagcaattgt cgggcaaggg cttgatccca ccaatattgc
     5881 tgctggaact taaattgatg tacccaatgt gaaagtggcg ctcggtattc atttaccctc
     5941 actaatttat gccacttcgg ttcatttttc aaacaatagc cacaagcgtt tcgaaactcc
     6001 accgcactca ttccacaatg acgacaataa ggggttggtt ttagcaattt ttggcaatgg
     6061 ctgcaaatgc cttgcacgcc aatagcgagt ggatgattac aatgaaagca acggaagccg
     6121 aataaattca catttcctcc ttttatttgc agattatcaa atttagcctt attttttccg
     6181 gctatttttg tgacatcgct cgaaaaaaga gtaaaatctc attatcagta aataaaaagg
     6241 aaaccgagat gaaatccctc caaaaaccga tcaaaattgt ctttttcgat attgatgaca
     6301 ccctctatgt aaaacataaa gcctatattc caaaatcaat taccgagcag gtattacctc
     6361 gtttaaaagc caaaggcatt attccggcca ttgccaccgg cagaaatcac ggctgttttc
     6421 ctgaagctct caaaccttat ttaaaccctg aaatcggttt tgagctgttt gttaccatta
     6481 acgggcaata taatttctat aaagatcaaa taattagtga ttattcgtta agccgtgagc
     6541 aaattgaacg tattatcgaa aaagcgaatg ctttgggtat tctgtatgcc tttattacgc
     6601 caatgacgat tgcagtttcc gaaaccaatc cgattgtgca cgaagcggtt ttaccgatca
     6661 caccgaatta tttgatcgat ccacactact acgaaaaaga gaaaattgtg caaatgttac
     6721 tgttctacac cgaagagcag aaacaagcgg tcgaaaatag tggcattttt gcaaatgatt
     6781 taaaagaggc tcgctggcac cccaatgcgg tagatattct gcgaaaagaa aattcgaaag
     6841 ctcgtggcat tcaagatgta ttagcaacct tgaatattga gattgaaagt gcgatggcat
     6901 ttggcgatgg tattaacgac ttggaaatgc tcccgaccgt aggtgttggt gtggcaatgg
     6961 gtaatggcgt gccggaatta aaagcggtgg cagatttcat caccaagccg cttgaggaag
     7021 acggcattct atacgctctt gaaactcttg aggtgattta aaagcaagcg gttgtaagtt
     7081 tttctccatt tacaaccgct tgtagtttct aattacgcct aaatagctta tcgcctgcaa
     7141 tcgctaaaat cgccccactg acgaccacca acgccccaag ataactgatc caattcatat
     7201 ccggcttggc aaaaatatgc ggataaaaat agtagctaag gttggaaaag atcatcgtaa
     7261 aaatcggcaa catcgtggtt acaatgctaa cctttgaagt atcccaatga ttcaacgcct
     7321 caccgtatga accgtagcca attaaggtgt tcaacagcaa taaataaagc aaccccacag
     7381 aaaaccatcc tcaagattgc ctaactgtgc cggtgaggca aacggtaaca tcagcagaga
     7441 acagccgatg taaataataa acagcacttg ctgagagtta aatttcgcta ataataattt
     7501 ttgagccacg ccgtatagca cccaaatcaa gctggctccg gcactcaata acactccgag
     7561 agcgtaggtg ttgagctgta aaatttcagc aaatttatcg ttaaaaaatg cgacaagccc
     7621 tgcaatcaat aacacaaatc caatcttttg aaacaagccg aacttttcct taaacaataa
     7681 tacgcctagt agaatcatcg aaaatggagc taactgccac aatacttggt taatggtggg
     7741 cgaaacatag tgtagagcct ctgcaaacaa aaagaaattt gccgacagac cacacacccc
     7801 taacaaaatt aaaccgagtt cttttttgct acaagcggtc aatttgggta aacgttttgc
     7861 aaatcctaga atcagcaata gccctactga tgccacaacg aacctaaccc aaactaaagt
     7921 ttgggcatct atcactttca atacttgctg ggcggcaatc ggtagcgttc cccaggtcat
     7981 tgctgcggtt aatgcaagtg aaaagccaag taaggggcgt tgtttcatcg tggaaattcc
     8041 tttttctatt ctatgtttct atattttaga aagattgaga tacaaaaaaa ccccgccatt
     8101 ggcgaggtct cttattgatt ttgaaatgat tagtcagcaa tgatgcttac atatttacgg
     8161 cttttctcgc ctttcacttc aaatttaact ttaccgtcag cagttgcaaa taaagtgtgg
     8221 tctttaccca tacctacatt tgaacctgcg tggaatttag taccacgttg acgaacgata
     8281 atgctacctg ctaatacaga ttcaccaccg aaacgtttaa caccaaggcg tttagcttca
     8341 gaatcacgac cgttacgagt tgaaccacca gcttttttag ttgccatctt ctagatctcc
     8401 tctgaaatta tgcttgaatc ccagtgattt tcacttctgt gaaccactga cgatgacctt
     8461 gttgtttacg gctgtgttta cgacgacgga acttaacgat tttaactttc tcgccacgac
     8521 cttgtgctac aacttcagcc actacttttg cacctgctac aaccggtgca ccgattttaa
     8581 catcttcacc gttaacgacc attaacactg agtcaaattc aactttctca ccagttgcaa
     8641 gttcaagttt ttctaaacga actacttgac cttcacttac tcggtgttgt ttgccgccac
     8701 tttggaaaac tgcgtacata tttactccgc tatgtgccaa ctgcttttgc ttttaggcac
     8761 taaattaatt cataaaaata gggttgcaat tctacggaaa aagattcgaa agagcaagag
     8821 cgattttgag aattttttga aatttgcaaa aaatggtaaa aatactaccg cttgtttcaa
     8881 ctgcgactaa aaaacaatag actgctccga ttttacaaca aaatcataat tcttttttga
     8941 acacaggcta aattaaggta gaatctactc aattttcgtc ttactttatg gattcgatgg
     9001 ctgtgcaact tttttcttta gaacaaattc aagcccttgc gaatgaagat atgcaagcgg
     9061 taaatgccga aattttagct caacttaatt ccgaagtgct actgattaac cagctcggac
     9121 attatatcat cgcaggcggt gggaaacgta ttcgcccgtt aattgcggtg cttgcggctc
     9181 gtgccttggg ttatcgtggt aaaaagcata tcacggcggc tgcttttatt gagtttgtgc
     9241 atactgctac acttttgcac gatgatgtag tcgatgaatc cgatatgcgt cgtggcagac
     9301 caaccgcaaa cgcccatttt ggcaatgcgg ctagtgtatt ggtaggggat ttcatctata
     9361 cccgttcgtt ccagatgatg acggatttag actctctgcg tgtgttaaaa gtgatgtctg
     9421 ccgccaccaa tgcgttggca gaaggggaag ttcagcaatt aatgaacgtg aacgacccaa
     9481 acaccactga agcgaactat atgcgggtca tttacaataa aaccgcccgc ttgtttgaag
     9541 ccgccactca atgcgtggcg attgcggctg atgcttctga ggaaattgaa accgcattaa
     9601 aaaattacgg ttgctactta ggcacggcat tccagctgat tgacgatatt ttagactact
     9661 ccgctcaaag cgaaagttta ggcaaaaacg taggcgatga ctttgtggaa ggtaagccaa
     9721 ccttgccatt gctccacgca atgcacgctg ccgacaaagc aggcaaaact actcaagcag
     9781 agctgattcg caatgctatc gaaaacggcg gcgaattgga acaaattcca caagtattgg
     9841 caattatgca agaacatcaa tctctggatt atgccatgca aaaagcccgt gaagaagccc
     9901 aaaaagcggt ggattcatta ggggttttac cggaaagcga gtacaaacaa gccttggttt
     9961 cattggcgta tttatcggtg gatcgctctt attaatttgc aaaaaatcat ataaatttaa
    10021 ccacttgtag gggcaaatga tatttgcccc ataacctata attcttttgg gcaaatgtta
    10081 tttgccccaa cataacaatg actgaacaaa accaacgacc ccaagaacat attgaaagcg
    10141 tacgagcgag acgccgtgct caaaatcaga aaaaaggccc gaatgccccg tttgtgcgtg
    10201 agaaattaac gctgccaaac ggacataaca aattactgct gcactcttgc tgtgcccctt
    10261 gctcgggtga ggtaatggag gctattcacg cctccggcat tgagtttacg atctattttt
    10321 ataacccgaa cattcacccg ctgaaagagt atttgatccg caaggaagag aatattcgct
    10381 ttgccgaaaa gtggggtatt ccgtttatcg atttagacga tgactacgaa aacgaccgca
    10441 aagagtggtt caaaaaagcc aaaggaatgg aatgggagcc ggaacgaggc attcgttgca
    10501 caatgtgctt tgatatgcgg tttgaaaaag cagcggaata cgctcacaac aacggtttcc
    10561 ctgtatttac cagttgctta ggcatttccc gatggaaaga tatgaaccaa atcaacgact
    10621 gtggtcaccg tgcagcagcc aaatttgatg acgtggttta ctgggactac aactggcgta
    10681 aaggcggcgg ctcacaacga atgatcgaaa tcagcaaacg tgaacgtttc taccagcaag
    10741 aatactgcgg ttgcgtctat tcgttaagag atagcaacaa atggcggttg gacacagggc
    10801 gtcagcggat tgaaatcggt aagctctatt actcacccga ttaaatgtta cttttctttg
    10861 cttcgccaaa gaaaagtaac caaaagaaag gcgacccgaa cttcgcttag ttacgatttc
    10921 cttaacggaa aatttgtaaa ctcgctacgc tcaaacagta caaattttcc tagaaatcgc
    10981 aagtcgctca gacgggacaa gcggtacatt attcaaaatt ttttacaaaa tatagtgatg
    11041 tactcttccc ttctgaaccg cccgaacgtg tttgaattgg taggaaaatt tactctgagc
    11101 gaagcgaatg taaattttcc gtgaaaccaa ttcaaactaa gtgaggataa gcggtgaagt
    11161 aggggtgttt ctttggttac tttctttgca catgcaaaga aagtaacgaa aactcagata
    11221 tagatttcaa cttaaaaatt tacagaagga ataataacaa atgtcaaaat tcgctatggt
    11281 tttccccggt caaggctcac aagcggttgg aatgcttgcc gaccttgcgc cacaatatcc
    11341 aatcgtggaa gaaaccttca aacaagcaag tgatgtgctt ggctatgact tatgggattt
    11401 agtgcaaaac ggtactgccg aagagttagg gcaaacccac cgcactcaac ctgcgttgct
    11461 tgcctcaagc gtggcacttt tccgcatttg gcaacaaaaa ttccctgagc aaaaaccagc
    11521 cgtgatggca gggcatagtt tgggtgaata ctcagcgttg gtgtgtgccg gtgtgttgga
    11581 tttccaagat gcgattaaat tggtcgagtt gcgtggtcgt gcaatgcaag aagccgtgcc
    11641 ggcaggcagc ggtgcgatgt ttgcgattat cggtttagat aacgaaagca ttattaaagc
    11701 gtgcgagcaa gcagcaagcg agaccggtga aattgtctct gcggtaaact ttaacagccc
    11761 ggggcaagtt gtgattgccg gcacaaaagc agcggcagaa aaagcaggag aattgtgtaa
    11821 agccgcaggt gcaaaacgtg cgttaccatt agcggtgagc gtaccttcac actgtgcttt
    11881 aatgaaaccg gcggcagaaa aattagccga agccttgcaa aatattgagc taaatcaacc
    11941 gcttgtccct gttatcaaca acgtggatgt ggcagtggaa acagatgccg ataccattcg
    12001 taatgcactt gttcgccaac tttacagccc tgttcgttgg acggaaaccg ttgaaaaaat
    12061 ggcacaagat ggcatcacta ctttatatga aatcgggcca aacaaagtcc taaccggttt
    12121 agcaggtcgt attgtaaaag aattatcagc caaagcggta aatgatgttg ccagctttga
    12181 ggcggttgag gcttaatttt cgggagcgta aaatgagtaa agaaacccta tttatgctaa
    12241 taagtgcatt gattgcctta gtgattttac ctttagctta tactgttggt atcgctaaag
    12301 aaacattagg cggaaatatg ggaatgttat ccattgctgc cggttgctca tttttagcct
    12361 attttttaag tctaaacccg aaaattcgcc acgcctttat cggaggttca gctattacag
    12421 ctgctgctat tcaagcttgg gtattatatg gtgtgctatt aagcgaaaat gaccccaaaa
    12481 gtgcggtcgt tttatttcaa atttatctgt ttagtagttt agggccactg tttttttcag
    12541 ctatttgggc atttactcgc ccacgttgga tttaccctaa acaaggtagt gcggtgaaat
    12601 atgccttatt tggtggctta acgaccgtga taaactactt gattaccctt tttatttttt
    12661 atattctgac gttataacgt catactaagg agaatttatg agcaaaatcg cattagttac
    12721 aggggcaacc cgtggaatcg gcaaagcgat tgccgaagaa ttggtagcaa aaggctacat
    12781 cgtagtcggc accgcaacct ctgaaaaagg ggcggaatct atttcagctt atttaggcga
    12841 gaacggcaaa ggcttagtgc taaatgttgc cgatgcagag tcgattgaga gcgtattaaa
    12901 gcagatcaaa gagacctttg gcgatgtgga cgtgttagtc aataacgccg gcattacccg
    12961 tgacggctta ttaatgcgta tgaaagatga ggattggttc gacatcattc aaactaactt
    13021 aacttcggtt tatcgtttat ccaaagcggt attacgcccg atgatgaaaa aaggcggacg
    13081 cattatcacg atcggttctg tggtcggctc aatgggcaac ccggggcaaa ccaactactg
    13141 tgctgccaaa gcaggcttag ttggtttttc taaatcttta gcgaaagaag tagcaagccg
    13201 cggcattacg gtaaacgtgg ttgctccggg ctttatcgcc actgatatga ccgatgagct
    13261 aaccgaagat cagaaaaatg cgattttaag ccaaattcct gccggtgaac tcggttctcc
    13321 acaagatatt gccaaagcag tcgctttctt agccagcgat gatgcccgtt atatcaacgg
    13381 tgaaaccatt cacgttaatg gcggtttata tatgagctaa tccgctacaa gcggtctttt
    13441 ttcaaccgag ctttgtaaat tcagagttcg gttttgttat ttgttatttt gtgttatcac
    13501 ctgctttata ggtataggtg aaaaagtgtg aatatgttat gactagaaat acattagtag
    13561 taggcttttt attgtttgct attttctttg gtgccggcaa tctgattttc ccaccaaaat
    13621 taggttatga aagtagtaca gaatttttct cagccatttc aggctttgtt ttaactggag
    13681 tagggcttcc tctactcggt attatcgtca gtgcatttta taatggaggc tataaatctg
    13741 cattagacaa aatccaccct tggttttctt tagcattcct aacttctatc tatttagcga
    13801 tcggtccatt ctttgccggt ccgcgcacgg cagcaactgc ctatgaaatg gcgataatgc
    13861 cgtttttagg ggattccagc caagtgtcaa tgttaatttt taccttaatt tactttgcct
    13921 ttaccctatg gttagcgcta aatccaaata aaatggttga tcgcattggt gcaattctta
    13981 ccccgatctt gcttataacc atcattggat tagttattcg agccggcttt attttagata
    14041 ccgatccaca aatcactacg cctaatacga atgaacaatc ttactttttt aaaggtatgg
    14101 ttgatggcta tctaactatg gatgcacttg cttcgttagc ctattcggta attgtattaa
    14161 atgctattcg tgggaaaggg gtaagccaag ccgcattaca acgccaaaca gtttctgccg
    14221 gcttagttgc agcagttgct ctagctgtaa tttatatttc attgggttgg attggtaata
    14281 atatgccaat tagtgcaaac acactttcga tgttatcaga aagaaaattg gatctaggta
    14341 cttacattct taacactgtg actacccaag ccttcggtga actcggacgt agtgtcttag
    14401 gagtgattgt ttcacttgcc tgtttaacca ctgctgtcgg tttagccgta tcagtcagtg
    14461 aatactttaa tgaaattttc ccgaaaattt catataaagt ttatgctatc ttatttactg
    14521 tgattagttt tactgttgct aaccaaggat taaaagaagt tattagcaaa tccattccgg
    14581 tattattagt tctctaccca atagcaatga ctgtcatttt aatgttgctg attaatttag
    14641 tgattaaact tccgttgtct gcacaacgtc tatcactcct attagtgaca attgtagcga
    14701 tagcttcagt tgccggtgtc gggtttaccg aaaacttgcc tctaaaatcc tactcgatgg
    14761 aatggctacc tttcgctatc attggagcta ttattggagc aattatccac ccgcttaccg
    14821 gtcgaaagca taaataaagt aaaggcagtg agcattcact gcctcttttt taggagagaa
    14881 taatgcctga attacccgaa gttgaaacca gcgttcgtgg tgtatcgccc tatttggttg
    14941 ggcaaactat tgaacaaatc atcgttcgtc agccaaagtt acgttggacg gtgagtcctg
    15001 aattaagcca aatggcaggg gcaaaaatta ccgcagtaga aagacgtgcc aaatatctga
    15061 ttatccgcac cgacaaaggc gatattttgg tgcatctggg aatgtccggc tcgctcggga
    15121 ttttgcaaga acgtcaacaa aaagaaattg gcaaacacga ccacgtcgat cttattaccc
    15181 aaaacggcac aattttacgc tataacgacc ctcgcaaatt cggctgttgg ctatgggcag
    15241 aaaaagcaga agatcacgaa ctgttgcgta aactcgggcc tgaaccgctt tcaaacgatt
    15301 tcacagcaga ttacttattt gcaaaaagcc gcaaaaaaac gaccgcttgt aaaaccttta
    15361 ttatgaccaa tgaagtggtt gttggggtgg ggaatatcta tgcgtgcgaa agtttattta
    15421 tggcaggcat tcaccctgag cttgcagcac aaaacctgac taaaaaacag tgcgaacgtt
    15481 tagtaaaaac catcaaagaa gtgctgacta aagcgattat tcaaggcggc acaacactga
    15541 aagattttat ccaacctgac ggcaaaccag gctattttgc tcaagtgttg caagtgtatg
    15601 gaagaaaagg ggaaacctgt aatgactgcg gaaagccgat tgaaactaaa gtaatcgggc
    15661 aacgtaatac ctatttttgc ccgaagtgtc agagattgcc gaagtaatct aataataaac
    15721 tctctccccc tgagggagag agacagcttg ttgaaacgtt aagtgaaaca agcagagaga
    15781 gggtaaaacc taaacctgca accaaaaggt aacaggcccg tcattttgta ggctgacttg
    15841 catatcggct gcaaattgcc cggtttgggt atgaatctta gtggcggctt gttgatgaaa
    15901 gtaggcatac aaacgttcgg catcaaccgg gcttgctcct tttgagaaac taggacgcaa
    15961 gcctttattg gtatccgccg caagggtaaa ctgtgaaacc actaacaaac tgccacctgc
    16021 ttgctgtacg tttaagttca ttttcccctc gctatccgaa aacacccgat aattcagcac
    16081 tttttccagt agcttatctg ctttcgcttc atcatcgtct ttttccacac cgagcagcac
    16141 caacaaaccg cttgcaattt cgccaacgac ttgcccttca acctccactt ttgcgaattt
    16201 aacccgctga attaagccta tcattaatgc tccccttgtc tgtttccatt tcgtctaaaa
    16261 ccgccgcaaa ttgcccgcca agtaatacca cctgccaact taactgaatc cataacagca
    16321 taatcggtaa caccgctaat gctccataaa ttgcctgata agacgggaag gcagtaatat
    16381 accaaataaa ggctttcttc cctaaagtaa agaaaatcgc tgccattaat gccccgatag
    16441 cagcgtgttg aaattttacc tttctaattg gaataaattt atacactaag gtaaaaagta
    16501 gccaaattag caaaaacggc atcaattcta ataaatgctg cccgagcgat aacgctccat
    16561 ttccattaaa aatagaaagc gacattacat aggtgctaat cgcaatgcta gcaccggcaa
    16621 gcagcgggcc aaacagcaaa atgagaaagt aaagcaagaa agagaaccac atcggacgct
    16681 gacgattatc gtgccaaatg gtattcaatg cctcatcaat actcttaatc agcataatag
    16741 taaccacaaa taagccgata aaacttacca gccccatttg tttggcatta ctgacaaaca
    16801 actcaatgta ttccgacacc atctcgctcg cactcggggc aaagttgtca taaatgaacg
    16861 cttttaactg ctcgtttgct tcgtggaaaa tcggaaaaaa ggtaaaaatg gaaaacagca
    16921 cgacaataaa cggcactatc gccagcattg tgctataagt gagataaccg gctgaaatcg
    16981 caatgttatt ttcattgtaa cgcttggcaa agagcttaat aaatctccac agtcgattta
    17041 acatcttaac ctctcaccaa ataatcgcct tcacacaccc atttgtaggt ggttaaggct
    17101 tctaatccca tcgggcctcg tgcgtgtaat ttctgcgtgc taactgccac ttctgcccct
    17161 aaaccaaact caccaccatc ggtaaaacgg gtgctggcat taatataaac cgccgcggca
    17221 tcaacctgtt gtacaaattg acgggcaagt tgataatcgg aggttaaaat cccctctgaa
    17281 tgctgcgaac cataatggcg gatatgctct accgcttgtt gcaggtcatt caccaccacc
    17341 acgttaagat caagcgataa ccactcttgg ctcaattttg cttgatcaag cggttcaatt
    17401 ccttcaattt tttgcaaatc gttactgtga atagtaacgc caagctgttg cattttggcg
    17461 accaatttcg gcaaaaattc caccgcaatc gctttttcca ccaacagcgt ttccagcgta
    17521 ttgcaagtgc ttgggcgttg cgttttggca ttagcgatga cctctagaga tttctcctga
    17581 tccgctgttt tttccacaaa cagatgacaa accccaatcc cccccacaat caccggaatg
    17641 gtcgaatttt ctttacagaa ttgatgtaaa cctgcaccgc ctcgagggat caccatatca
    17701 atgtagcgat ctagcttcaa tagttccaat aataaggctc gatccggatc cgtcaccgct
    17761 tgcactgcca atttcggcaa gccggctttt tctaacgcat cttgcactac atcaaccaac
    17821 acagcattgg taaatttggt ctctttaccg ccacgcaaaa tcactgcatt gccggttttt
    17881 aggcataatg atgccacatc aatggtcaca ttcgggcgag cttcgtaaat ggttaaaatc
    17941 accccgagcg gcacacgctg gcgttcgatt ttcaaaccgc tgtctaacac gccaccgtcc
    18001 atcacctgcc ccaccggatc cgctaatttg gcaacatttc gcacatcgtt agcaatgtct
    18061 tttagccgat cttcattcaa taataagcgg tcaataatcg ccaatgaaat cccttgtgct
    18121 tgagcaaatt caatatcttt ggcattagcg tataaaattt cttctgcacg ttgctcaagg
    18181 ctatcggcaa tcgtgagtaa ggcttggttt ttctgtaggt tgccaaattg agccagctca
    18241 aaactagctt gtttggcttg ctgggcaagt tctatcatct gcataaatta ggtctcattt
    18301 aatcaatatt ctccataatc taacacgttc ttttatgtga agaaatatgc aaaagccaag
    18361 aaaattggat aaaatggggg aattttaaaa cattacgcaa ttcccctctc cctgattttt
    18421 ctcctgaacg gagaaaaatc tatccctctc ctacaagggg agagggtaga atttaactct
    18481 ctccccttgt gggagagaga ccgcctacag attcgttcag aatctgtagg cagagagagg
    18541 ggtaaaaaca gataaaactt aaacaaaatg ataaacacta cccctaaaca gctaatccac
    18601 attcttccgc ctcaactggc aaaccaaatt gctgccggcg aggtggtgga acgccctgcg
    18661 tctgtggtaa aggagctagt ggaaaacagt ttagatgccg gagccaatac tattcaaatc
    18721 gacattgaaa aaggcggctc tcagcttatc cgcattcgag ataacggttg tggtatcagc
    18781 aagcaagatt taccgctggc acttgctcgc cacgctacga gcaaaatcag cagcttggaa
    18841 gatttagaag ccattttaag tttaggcttt cggggcgaag cattggcaag tatcagctcg
    18901 gtttcacgtt taacactgac ctctcgtacc gcagagcaaa ccgaagcatg gcaagcctat
    18961 gcacaagggc gggaaatggc ggtggaaatt cagcctgcct cacacccgat tggcacaacc
    19021 attgaagtag caaatctgtt ttttaacaca ccggcacggc gtaaatttct acgcaccgat
    19081 aaaaccgaat ttgcccatat tgacgaggtg gttcgccgca ttgcccttgc caagccgaat
    19141 atcagtttca gcttaagcca taatggtaaa atcgtccgcc aatatcgcaa agtgcaagac
    19201 aattcggtag agcagcaaca aaaacgggtt gcagccattt gcggtgatga gtttattcaa
    19261 cattcaactt atattgacag gcaacacggg gatctgcacc tacacggctg ggttggctca
    19321 ccggctcttg cccgtctgca aaacgacctt tgctacagct atgtgaacgg gcgaatgatg
    19381 cgggataaaa ccatcaatca cgccattcgc caagcctacg gcgacagcat tgcaagtggt
    19441 tactatccgg cttttgtgat ctttttggat ttagatccga caatggttga tgtaaacgtt
    19501 cacccggcta aacacgaggt gcgctttcat caaggaagac tggtacacga ctttattttg
    19561 caaggcgtac tcaacagcct gcaacaaacc caacctacgc ttgaactgcg aaatgaagtg
    19621 aacgagccgt taccgagcta tgttcaagac actaaccgcc aagctgcggg gcaaaatatt
    19681 tttgtgtcgc aaaccgctcc acaagcggcc gaatttacgc aaaaaaatgc aagtcgatca
    19741 agctatacgc ctaaatttga acgaagcaac agtgtgagtt cgtccgctca aaaatggtat
    19801 aaggaattgg ttggtggcga tttgcaccaa acaacgccaa ctatcaaaac gcaaagcacg
    19861 cttgttgagc caaaaacaga accccaaact gaacataaaa cagaagccgt ttcgcaaaaa
    19921 attacgcaaa ttgcaccgct tgcaagccac cattcacaag ccttagcgat tgtaaaaaac
    19981 caagcggtgt taattaaaga agaggaaaat ttttacctgc ttccactggc aacattagcc
    20041 gagcttaaac tcaagctaca actggaaaaa gggcaatctc aagcgttgtt gatcccactg
    20101 aatctcagtt tagatcaaca acaaaccgaa cgctggcaac aaaccaaaga agaactaact
    20161 aagcttggct tttcgatcag cgaaaaacag tggcaagccc aaacacggct cacagtgctt
    20221 tccgtaccac aggctgtcag agaacaaaac ctgcaacagc ttttatttgc tctgttcaac
    20281 caaacacaag cggtcaattt agccgaattt tttgcaaaaa aatgcgaaaa acccaccgct
    20341 tgtacgctaa gtgacgtgat tgctctactc tcagaaattg agctttatgc caatgggaaa
    20401 aaagagctgg agggaataag gaaggaagtg gatttttcca agtttttata gtctaaaggg
    20461 caataccctt tagccaaata aatgagagtg gctgccaatt cgcagcagct ctagcttatc
    20521 atcttccact tttcgataga taagaaccaa atcaggttgg atatggcaat ccctaaagcc
    20581 tttccattcg ccggttaaag cgtgatcgca gtatttctca ggtaatggct tgtcattaat
    20641 cagaaaatac aacgcctcaa ttaatgaaga ggataaagac tcaaccgcac attttttata
    20701 gtcacgttta aaagcattac tatactcaat ctctcgcatt gagttgctcc attaaatccg
    20761 caatactatt tgctgaatgt agcttttcct cgccattctt aacagccctt aatacttctg
    20821 ctgtctcaaa attaggctga taatcaaact gaaaacgttt ttctgttgcg attttcgcta
    20881 atgtcattcg catcacatca gagacagtca agccgatacc ggctaacacc tcacttgctt
    20941 gctctttaat ttcagggctg attcttacgc ttactaatgc actatttgcc atcaaaaact
    21001 ccttttgtat atcaaatgat atacaaaata acatttaaca aaaaattgtg caataaaaaa
    21061 gaaattcaaa gaaaagaaat gaatagttaa aattagctcg ttacaagaaa tcattgagtt
    21121 actcaagaaa tcaaggtaat ataaagaagt ctgggcggaa cacgaacccg cccctagcat
    21181 ctagccgctt acaagaacag atagattagt tgaaagagcg tgaaaatttc aactgctgca
    21241 cacagaaacc caagtgcaac taaaatcaat agtagttctt tcattgtaag cctccttagt
    21301 gctactaagg atgatttagg ctcaggctac tgcgaatagc cctaacgagc ctaccagtag
    21361 cacaatcagg attgtaacaa aaaagctgtg aatttcacgg ctttttttgt atctatatac
    21421 agtatattaa tatgaataaa aaaccccttg cccttttttt aatggggccg actgcctccg
    21481 ggaaaaccga tttagctatt gctttacgcc aagaattgcc ggttgaggta atcagtgttg
    21541 attctgcctt aatttacaaa ggaatggata ttggcacggc aaagccaagc aaggaagagc
    21601 ttgcccttgc accgcatcgc ttgattgata ttttagaccc tgccgagagc tattcggcga
    21661 tgaatttcca cgctgatgcg ttgcgtgaaa tggcagatat tacctcacag ggtaaaattc
    21721 cgctcttggt gggtggcaca atgctttatt acaaggcatt gcttgaaggg ttatctcccc
    21781 tgccctctgc cgatccagcc attcgggctg aaatcgaaag ccgagcggca caacacggct
    21841 gggcaacatt gcatcaagaa ttagtgaaaa tcgacccgat tgccggtgca agaattaacc
    21901 caaatgacag ccaacggatc aaccgagcgt tagaagtttt ttacattacc ggcaaaacga
    21961 tgaccgaact cactgctcag caaggtgagg ctctacctta taatatctta caatttgcaa
    22021 tcgctcctca agatcgtgca gttctacacc aacggattga acaacgcttt cacaaaatga
    22081 ttgagctagg ctttgaagag gaggtcaagc ggttattttt acgcaaagat ttgcatatta
    22141 atttgccctc gattcgctgt gtgggctacc gccaaatgtg ggaatatttg caaggcgata
    22201 tttcgctcga tgaggcgatc tttaaaggca tttgtgccac ccgccagctg gcaaaacggc
    22261 agattacttg gctgcgtggc tggcaaggcg aactgacttg gttagatagt ttagctccga
    22321 ccagttctaa agcaaaaatg gtggaaaaga tcgatcatta tttaaagaat tgtgataata
    22381 tctaaacgcc tttggcactt atcgcattat ttggtgtctt aaaaatcaca caactataaa
    22441 aaggaaaata acaatggcaa aaggtcaatc tttacaagat ccatatttga atgcacttcg
    22501 tcgtgagcgt attcctgttt caatttattt agttaacggc attaaattac aaggtcagat
    22561 cgagtcgttc gaccaatttg tgattttatt aaaaaacact gtaagccaaa tggtgtataa
    22621 acacgctatt tcaacggttg ttcctgcccg tgcgttatcg cataacaata acaaccaagc
    22681 ccaacacgct cagcctgctg ccgaagtaac tgaacaagcg gaataataac attattactc
    22741 aaccaatgtt aaacgaatca ttgccaaact cagaagaaaa ttctgcgttt ggctttgctt
    22801 tatctgaagc aagccaaact acagaagccg cctcgcctac cgatccagaa aatcaagata
    22861 aagccatttt ggtgcatctc tatctcgggc aacataaaga tgttgaaaat ttgctggaat
    22921 ttaaaacgct ggcagaatct gccggtgtcg aagtgctggc aacgcttacc accagccgcc
    22981 aaagttcgca tatcaaatat tatgtggggc aaggcaaagc ggagggaatt gccgaagcgg
    23041 tagaacgact cggggcaaca gtagtgctgg tgaaccatca acttagcccg gctcagaccc
    23101 gtaatttgca atcgttatgc ggctgccgtg tggtggatag aaccggcttg attttggata
    23161 ttttcgctca acgggcaaga tcgcacgaag gcaaactaca agtcgagctg gcacaacttc
    23221 gccatttatc gacccgtttg gttcgccgag ttactaatca agaccaacaa aaaggcggag
    23281 cagttggctt gcgtggtcca ggtgaaacac agctcgaaac cgatcgccgt ttgattaaag
    23341 tgcgaatcca acagttactc aaccgcttgg aaaaagtcag caagcaacgc aatcaaaatc
    23401 gaaaaaccag acaaaaagcc gatattccaa cagtatcctt agtgggctat accaatgccg
    23461 gcaaatctac cctgtttaat gcgattaccc aagctggtgt ttatgctgcc gatcaacttt
    23521 ttgcgaccct tgacccgact ttacgccgga ttcaggtgca agatgtcggc acgacaattc
    23581 ttgccgatac ggtaggtttt atccgttttc taccgcacga tttagtttca gcctttaaat
    23641 ctaccctgca agaaaccacc gaagctactt tactgctcca cgtcattgac ggctcagacg
    23701 aacgcaaaaa cgaaaatatt gatgcggtaa atcaagtgtt ggacgaaatc ggagcgttag
    23761 atattcctac tttattggta ttcaataaaa ttgataaatt agaaggtgta gaaccgcata
    23821 ttgaacgcaa tgccgatggc acgccagttg ccgtgtatct ttccgcccaa gaaaatagcg
    23881 gcattgagct gttattccaa gccattcaag aaaggcttcg cagtaactta gtgtgtgaaa
    23941 acttgctcct cccggttact gccggtgcaa tttacgcaca atttaaagca gaaaactgca
    24001 ttaaaaccga gcattttaat gagttcggtg aacggattat ctctattgaa attaacgaag
    24061 tgcaatggaa caaatgggtg aaacaatttc cggatctagc cgaatatatt gagctggcaa
    24121 aatgggggta attaatctca cttctgagta agttatattt aaattatgca agcggtcaat
    24181 tctttacgtt tttttgcaaa aaagtgtaaa gaattgaccg cttgttttat ccattaaatc
    24241 caatttagcc aataaaaaaa tccctcaaat aaaccagatt tccccgttct atttgaagga
    24301 ttttatttat tcttccccaa aagtggggaa gaaaagctta atcaagcagt tactaccact
    24361 gataacctac gcttgcagca gcacctgtat caccacggct gttagagtta acactgaatt
    24421 tgatacccac tttaccatta tctgataaac gagaataacc tacagcaata gctccttgac
    24481 cacggtaagc acctacaccg gctgaaacca tcgatttacc aggttgtgtt acgtgatata
    24541 aattacctgc ggctaatgca cctgcaatac ctgcacgtaa gtctttatct acgttactga
    24601 tacgattgtt cagacgattt tctgcacctt tcaactgatt cacattaaca gcatcagtgc
    24661 ctttcacacc cggagcaacg ttcgtaatac gtgtttgcgc attcttgcta cctgcaatgt
    24721 taagctcatt tacgccatct tttgcaacag ttgaactgat agttgtacca ccaactttca
    24781 ctgaatcaaa ttcaagattg cttgaggttg ccacttcaac agttgaacct ttacgtgaaa
    24841 caacgatgtt tttgccacct gaaacggcca ctttaccatt gttattaaca cgttgagcac
    24901 ctttctctga agtttcagct acattaccat caacagtgag ataccagcct gtattctgca
    24961 ttgcattata caactggcta ccgctcacaa catctttact tgttgccgtt acatcaccat
    25021 ctttaacgtt agtgatcttc gtgcctttcg catcaatacc gcctttagtt acacttgggc
    25081 cgtctttaat ggttaaacca ttgttattta cggttgtgtc gccagttgtt acagaaccat
    25141 cttccgttaa atcaattgct ttcgctaatt gtacctctaa tgtgtctgta ccattcgctt
    25201 tcacattgat gttgcctgat gctgacttaa agttttcaga agctgtttta tccacacctt
    25261 cacctttcac tttaacttca gtattcagtt tctgacggtt cttcacatct tcattgttac
    25321 cagagaagat taaaccatca tttaaggttg caacttgttc tgtgacagtt ttaccgtctt
    25381 tatctttggt ctcataaacg atacgagtct taccgttctc acccggtaat ccatctttac
    25441 catttgtacc atcaacacct tcagcaccat ctttcactgc aatatttgca cttgcaccat
    25501 ctttaccgtc agcgcctttc ggaccggtta agccgataga gccatcttta ccgtttaata
    25561 caacagaagc gccatcttta cctgtagctc caattgaacc atcaacaccg tctttaccgt
    25621 cacggcctgc agtaattgtg ccgtcacctt taatggttac gccatcttta ccgtctttgc
    25681 cagcaatacc gatttgaccg tctttaccat ctttaccacc aatggttaag ttgtttttca
    25741 agccgaaggt cacgttatta ccttctttag taataataat gttaccgtct ttgttgttta
    25801 gatcaactgt tgctcctggt ttaacgttgc ctttgtcttt accatttgct gttaagttcc
    25861 aacctgcgtt agcaacttct tgcacttgat ataactggct accatttacc gcatctttgc
    25921 tggttgcaga aatttcaccg tcttgaacgt taatgatttt cttatcgcct gcattgatac
    25981 cggctttagt tacacttggg ccatctttga tagttaaacc atcattattt accgtagtat
    26041 caccaatagt tacagaacca tctttcgtta aatcaatgtc tttcgctaat ttaactgtta
    26101 acgcaccatt gttgtttaca acaccgatat tgttatcgga caggttcgtt aaatcagtat
    26161 caccgcctga gatttttaac gtctcattaa gtttcttaac gatttcttta ccgtcatcac
    26221 ctacgaatct taagccatcg ttcagtgttg caacttcctc agttgtaccg tctggtttct
    26281 cataaacgat acgggttttg ctttcgccat ctttaccgtc gttaccgtca aggcctttag
    26341 caccgtcttt tacagagata gttgcacttg caccatcttt accgtcagcg cctttcggac
    26401 cggttaagcc gatagagcca tctttaccgt ttaaagtaac accaactgta ccatctgcac
    26461 ctttaacacc gattgaacca tctttacctg gaccgccaat ggtaaaatcg ttatttaacg
    26521 taaagtcaat cacattatca cttactgttg tgatgatgtt tttgtcttta gaggtgtact
    26581 taacagtttc acctagttta acgtgatcag tttcaccatt atccgctgca atgctaaagc
    26641 ctttctcgat aatatcgttg cttgcattta caacatcatt aatattcgca gcgttagtac
    26701 caacttcttt taattgatcg tctgttaagt cgttcactga agtgattggc ttaccatctt
    26761 taccgttaat gccgtcttta cctgctaaac cgtcaatacc gctcttcacg ttaacaattt
    26821 ggttgccgcc attgtctaga ccgtcttcag taagcttaac tgctttacca tctgtgccat
    26881 cttttggcgt gatagttacg ccattattat ttactactgt tgttggacct ttaccttctt
    26941 gaccattctc tccatcttgt ggtttgaatg tagctgagtt caagtcttta agatctttag
    27001 ctaattgaac agataattta ccatctttgc tgtttacgcc gatgttattt gcagttagct
    27061 tatctttatc agctccacca atgatttcta aggtttcatt caatttctta gatactagtg
    27121 ttgcatcatc acctttaaag tgtaaaccat catttaaagt agctacttgc tctggttcac
    27181 ctataggatc accattgcca tctgttggct gatattggat acgagttaca ccatctttac
    27241 ctggtaaacc atctttacca acaagccctg gtttgccctc agtaccttta atggttaaac
    27301 catctttacc atttgtaccg ttcaagccga ttgaaccatc tttaccgtta agaacaactg
    27361 cagaaccgtc tttaccgttt acaccgatag taccatcaat accatctttg ccagggcgac
    27421 ctacagtgat agctccatct ttaccatcta aagcaatagc atctttaccg tctttaccag
    27481 caataccaac tttacctggt tcaccatctt taccaacagt aatattattc actaggctaa
    27541 cttctaatgc atggccacca tctttctcaa caacgtttgt tttaacattg ccatcaccaa
    27601 tgatcttagc tgtcgtacct aagttttgct taaactcctt gttgttctca tcttttagac
    27661 caaacgcatc tgttgtcacc tcagtaacag tctcagtaac agtatttttc aagtcaccga
    27721 cgttaaccgc attgttaaga acatcacctg ctgcctcagt aagtttaaca ggttgaccat
    27781 ctttaccttt taagccgcta tcaacattgg tgatttggtt accaccgttg cttaagccat
    27841 cctctgttaa ggtaacatta gatttagttg catcattagg atctttctta gcaatcgtta
    27901 caccatcttt attgataact gtagaatcac cttgtgtgcc gaatgttgca ctatctaagc
    27961 ctgctagatc ttttgctaac gccacagtaa tggtattgcc aacaacctgt gtcatgatat
    28021 ttttaccagc atcaaactta gcccaatcag tgtttgttgt agcaccttta atatccacct
    28081 gcgtatttaa tttcgcatcg tgaactttgc catcattcgc accgaatttt aagccatcgt
    28141 taagtgttgc aacttcttct ttatcaccat ttggtttttc gtaaacaata cgagttttgc
    28201 tttcgccgtc tttaccgtcg ttgccgtcaa gacctttagc acctttctca actttaattg
    28261 atgctttacc atcttttcca tcttttccat ttacgccatc aacgccgtgg aatacgatgg
    28321 cttctggttt aattgttaca ccatctttac catcagcacc tttaacacca actgaaccat
    28381 ctttacctgg tttaccacca acagagatgt tatcggataa gccaaaatcg atggtattat
    28441 ttgttacggt tgtaacaaca ttcttatcgg ttgaggtgta cttaacagtt tcacctaact
    28501 tcacattgtc ttctttagca ccacctccta agccagagtg atccgctgta atgttaaagc
    28561 cagttttaac taaagtgtca gctactgcgt aaagttgtga accgttaatc gcatctgttg
    28621 aattggaagc aattttaccc ggtgcaacaa acttaatttg gcgagcattg gttgcattac
    28681 ccacactcac ataatcacca tcaacaacat tttcatcgcc agcaaagctg ctatatttta
    28741 atgtgccaac agttgcagta tcttctttag ttgcggttgc ttcagtagag tcagaaccta
    28801 gaattacaga accctttgtc ttaacagtaa cattagaacc taatacgaaa gtatctttac
    28861 ctgaaactgt attgttataa cctaaagccg cagcacgaat acccgaaaca gtactattta
    28921 caccggctgc aattgagttt ttaccctttg cggttgagtt gtaagcaata gcagttgcat
    28981 aattaccttt tgatttggcc tcaaaaccaa ttgctgtgcc accttcatgc gtaatattgg
    29041 cacgtgtacc tactgctgtc gcattcgcct tacttacatt agcactataa cctatcgcaa
    29101 cagaattatt accacttgaa ttagccgtat caccaattgc cattgcacca tctttgctcg
    29161 cgttagaatg cgaaccaata ctgatcactt tttctgcatg agagtttgca ttccaaccaa
    29221 tgctaatagc attacttttc caggctttag aagcattacc aaaagcaatc gcaccgattc
    29281 ctgttgtatt cgaatttaca ccaaaggcga tagaaccatc acctgttgca cttgcatttg
    29341 ttgaaaacgc caaagtatca ttcgaatttg cacgagtatt cgtacccaaa gcaaatgcat
    29401 tatttgctac tttcgctgta tcaggattaa ttacctgtcc taattcagaa atatttgaat
    29461 tctctaattt aaagccaccg gaccaagcgt tagtaccaat ggcaaatgaa tggttaccat
    29521 tagttttcgc tttagtaccg attgagaatg catctgttcc aaatgctaaa gtattatttt
    29581 gcccatcata aattgaacgt ctattttcat gaccgtaact tccatccact gtctgtggag
    29641 taccactacc aatcgcaaaa gaacggtcac ctagcgctgt agccgaagca ccaatggcga
    29701 agctagctgt agcatttgcc caagatgttg aaccaattgc agtagaataa tctgcaattg
    29761 cagcagattg acgcattgca gcaaatgagt taaatccttc cgcacgtgca gaggtaccta
    29821 atgctacaga accaatactt gcagtacgag aaaaagaacc aattgcaata gacaatcccc
    29881 ctccagaata tgaataatca ccaatggcta tgcctgacgc tttcggtttt cctctacttt
    29941 ttctgttatt gtctaaagct acagcattgt gtccaattac aattgctgag gtagtattag
    30001 acttatacaa taatctagaa tccttctcct tattaacaga gttattagat tcttgaagct
    30061 tagttctgtc tgcataggat aagctacctg ggttaaagta gttatatgga ttcgtaatag
    30121 aatctgtagc caaactagtg tttgtataaa tatctatacc accattaaca actttttttg
    30181 gtgctttttg ttgtacttct attctatcaa taatcaccgc cgcttctgcc ggtgtagcag
    30241 aaagcataaa ggctgccatt ccactgataa gggttgctcc ccctagaatg ctagccgctg
    30301 ctaatttaat agtaccgcgc ttatcagtag aagaagcggc tttaccttgc gatcgagcaa
    30361 gttcagacac aacttccatt ttttgcgtcg tttggttaaa aataacctta aatattttgt
    30421 tcataatttt atacctttag aattattata aagcttactt cgtaaataat tcggcaatta
    30481 caacccaccg aataaacaac cttactactc ttaatttagt aagaggaaag tcatatcgat
    30541 aatagtaaga ttttcacttc aaaagtgaaa caagcttttc actt
//
LOCUS       DANLXF010000003        27532 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.003, whole genome shotgun sequence.
ACCESSION   DANLXF010000003 DANLXF010000000
VERSION     DANLXF010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 27532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27532
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.003"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            122..850
                     /gene="fabG"
                     /locus_tag="PXD66_000080"
     CDS             122..850
                     /gene="fabG"
                     /locus_tag="PXD66_000080"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012771807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HDL6318424.1"
                     /translation="MNNTVLITGSSRGIGKAIALTLAQQGFDIVVHCRSRVEEAEAVA
                     QAVRNLGKNARVLQFDVADREKVRSILEADVEQHGAYYGVVLNAGLTRDNAFPALTDE
                     DWDSVLRTNLDGFYNVLHPIMMPMIRRRKAGRIVCITSVSGLIGNRGQVNYSASKAGI
                     IGAAKALAIELAKRKITVNCVAPGLIDTDILDENVPIEEILKMIPAGRMGDPDEVAHA
                     VAFLMDEKATYITRQVIAVNGGLC"
     gene            913..2058
                     /locus_tag="PXD66_000081"
     CDS             913..2058
                     /locus_tag="PXD66_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HDL6318425.1"
                     /translation="MKRVVITGIGVVTAFGKEWAEIHGYQPPKHWNRKQLRSLGRVSQ
                     FVVEASERALAQAGLLDENGVIFDWLKDRRMGVACGSSTGSTNDIKDAAELLMTGKSD
                     GFNANTYVRMMPHTTAANIGIFFGLTGRIIPTSSACSSGSQGIGYAYEAIKYGMIPMM
                     LAGGGEEFCASKVYVFDSLYAASRRNDNPKGTPRPYDKDRDGLVIGEGAGMFVLEELE
                     HALARGANIIAEVVGYGANSDGSHVTRPQATTMQRCMELALKDAGIQPNQVGYVNGHG
                     TATEQGDIAETQATTAVFGKIPLSSQKSYLGHTLGACGALESWFSIEMMRDGWFAPTL
                     NLENVDERCGDLDYLQGDGREIHTDYVMNNNFAFGGVNTSLIFKRWK"
     gene            2072..2977
                     /locus_tag="PXD66_000082"
                     /pseudo
     CDS             2072..2977
                     /locus_tag="PXD66_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251927.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dialkylresorcinol condensing enzyme"
     gene            3000..4142
                     /locus_tag="PXD66_000083"
     CDS             3000..4142
                     /locus_tag="PXD66_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HDL6318426.1"
                     /translation="MLSNVYITNTSAFLPNAPVSNDAMEAVLGMVGETPSRVRKMILR
                     SNGIQHRYYAIDPITRQATHSSTELAVQAVKKLGNSATSATCLAVGTSYPDQIMPGQG
                     VMVHGLLDAEPMEVLTTAGVCVAGMAAMKHAYNAVRIGEHRAAIATASETASAIMRSE
                     HFRAEIDHKKLDDARPEIGFEKDFLRWMLSDGAGAVLLANQPNEKGLSLKIHWIDLVS
                     YANEMPVCMYAGAEFIDGNYTGWKAMSEAQRAECSAMAVKQDVKLLNENIVKYTVEKA
                     LERIIPKYKLNAEEIDYFLPHYSSGSFRDKLLEGLQRINFLIPQEKWFTNLTTKGNTG
                     SASIYIMLDEFIRTHDLKQGQKILCYVPESGRFSSCFMLLEAVVNS"
     gene            4191..4406
                     /locus_tag="PXD66_000084"
     CDS             4191..4406
                     /locus_tag="PXD66_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL6318427.1"
                     /translation="MDINDIPQDNSKIFNGERKVIYARNPQPEIRQMISSILACYAWD
                     ENNPFVAKSDARLKALAEVVGKAEIGD"
     gene            4687..5178
                     /gene="rplJ"
                     /locus_tag="PXD66_000085"
     CDS             4687..5178
                     /gene="rplJ"
                     /locus_tag="PXD66_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDL6318428.1"
                     /translation="MALNLQDKQAIVAEVNEAAKGALSAVVADSRGVTVEKMTELRKA
                     AREAGVTMQVVRNTLLRRAVEGTEFECLTDTFTGPTLIAFSNEHPGAAARLFTEFAKT
                     NKEFELKGAAFEGKKQDVAFLATLPTYEEAIARLMGTMKEAAAGKLVRTLAALRDQME
                     AAA"
     gene            5237..5605
                     /gene="rplL"
                     /locus_tag="PXD66_000086"
     CDS             5237..5605
                     /gene="rplL"
                     /locus_tag="PXD66_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDL6318429.1"
                     /translation="MSLTNEQIIEAIASKSVSEIVELITAMEEKFGVSAAAAVAAAPA
                     AGAAAAEEKTEFDVVLAEAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGISK
                     AEAEALKKELEEAGAKVEIK"
     gene            complement(5712..6542)
                     /locus_tag="PXD66_000087"
     CDS             complement(5712..6542)
                     /locus_tag="PXD66_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL6318430.1"
                     /translation="MVQKPLNHYLEMQEHFLYVPYLHHHRRIRVLLPKDYHNEGWERY
                     PVLYMHDGQNVFYSKESYSGHSWKIIPTIKSHQELPKMIIVGIDNAGANRLNEYGPWK
                     TTTGSTPETRNAGGLGEEYAKWVVETVKPFIDIHYRTKPQQEHTLLAGSSMGGIITAY
                     MGSRYPHIFGHLGVFSLASWFSEPDFLRFTHQYPLQPNTKVFIQVGTNEGDEIDSHFI
                     SNTNQTYIDCSLNYYQALIRIGVPLDNIRLRIMANEIHHEMHWADHFVEFLHFSLLRK
                     "
     gene            6768..8309
                     /locus_tag="PXD66_000088"
     CDS             6768..8309
                     /locus_tag="PXD66_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HDL6318431.1"
                     /translation="MQASLQDILEVVKAKNLTYQQKLMSLGNIAERLFNPIELLGYTE
                     EEWEHIENQMICDLNEGYAIYRPRYILPDYNVYMQKGCQFLDLPPPTNLDEALDGLLI
                     LYSHVPSITTFPVYIGRLDQLLEPFITDEEQDYIKIKRFLNHIDKTISDSFCHANIGP
                     NDTKAGRLILKAVIELENITPNMTIRYDKTKTSREFAELAAKACLLVSKPSFANDAYY
                     IQDLGEEYGIASCYNALPECGGAYTLTRLRLGTIGRACASVEQMVNEMLPKVAKLALS
                     TMDKRHKFLVEESNFFESSFLVQEGFIKRTNFTSMIAIVGLADAVNHLLKQEGLNETF
                     GQSKRGDEIATLIMDKLQAVNDAHKGLYVERTNNQYLLHAQVGANNHAEDKMNTPAHR
                     IRVGEEPTLLAHLKQSAPFHKYFPSGTGDLFAFDQTYTDHLDAVVDIIDGSFAQGYRY
                     ITTYLKNTDLIRVTGYLVKKSEVERYRKGEAVLRDTTWYGSGTDDCAKVFDRQLRDEE
                     NVNAG"
     gene            8312..9100
                     /locus_tag="PXD66_000089"
     CDS             8312..9100
                     /locus_tag="PXD66_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HDL6318432.1"
                     /translation="MAALSEIFVPLHRIIPFSNVEGQGNRTSIFLQGCKLNCLYCHNP
                     ETIPRYSKEAKQVSLQYLYDQVMEAVPFIRGVTISGGEPTIHHKKLVPLFEALREQGL
                     TCYLDSSGFFDFEATEPLINVTDKFLFDLKGDGAGLQTLCFDRKNQAGKVPEQALPNV
                     NHIKPENLARNLQNLAKLLPLDKVEEVRLVYLNEFYNAHHLLEKVADLLKPYPDVLLK
                     IIRVHTKGARDETGLARFVPTIEQTDELAAFAKRHGIQKVITIY"
     gene            9113..10370
                     /locus_tag="PXD66_000090"
                     /pseudo
     CDS             9113..10370
                     /locus_tag="PXD66_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005760733.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
     gene            complement(10449..12458)
                     /locus_tag="PXD66_000091"
     CDS             complement(10449..12458)
                     /locus_tag="PXD66_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL6318433.1"
                     /translation="MSAENMPSVIRFEQAVAKKDYESACTELLSILSKLDSNFGGISN
                     IELNMPEQIENLENDKAIYFCTRMAVAITRLFEDPALEISEHGAMRFLTLQRWIALIF
                     ASSPYVNADHILRTYNRNKESANPNTVDLDATLQALIKFCILYLPESNILLNLDAAWN
                     ASSDLTASLCFALQSPRFIGTSSAFAKRAAILQWFPEKLAQIENLNKLPSAISHDVYM
                     HCSYDIEANKHNVKRSLNAVIRRHLLSVGWEDRKIEQLGTRNNKPVMVVLLEHFHSSH
                     SIYRTHSTSMVAAREHFHLIGLGSDAVDEMGQQVFDEFHLLPQDGSLFDRLSFLKDIC
                     DKNNPAVFYMPSIGMDLTTIFASNTRLAPIQAVALGHPATTHSDFIEYVIVEDDYVGS
                     ESCFSEQLLRLPKDALPYVPSALAPQNVVYNLRENPEVIHIGIASTTMKLNPYFLEAL
                     KAIRDRAKVKTHFHFALGQSSGITHPYVERFIKSYLGNDATAHPHSPYDEYLNILHNC
                     DMMLNPFPFGNTNGIIDMVTLGLVGVCKTGPEVHEHIDEGLFKRLGLPNWLITQTAEE
                     YVTQAIRLAENHEERLAIRRDIIENNKLQTLFSGDPRPMGQIFLAKVQAWLADKNSKN
                     AEVEVKTKKVRKAATASQSAKKQTTSKTQTAKAEKDNTAKTETKS"
     gene            complement(12468..13805)
                     /gene="rimO"
                     /locus_tag="PXD66_000092"
     CDS             complement(12468..13805)
                     /gene="rimO"
                     /locus_tag="PXD66_000092"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HDL6318434.1"
                     /translation="MSASPNIGFISLGCPKNLVDSERILTELRADGYNIIPSYENADL
                     VIVNTCGFIDSAVQESLESIGEALEANGKVIVTGCLGAKENQIREVHPKVLEITGPHS
                     YEAVMNHVHKYVPKPEFNPYTSLVPKQGIKLTPKHYAYLKISEGCDHRCTFCIIPSMR
                     GDLASRSIVQVLDEAKRLVDAGVKELLIVSQDTSAYSLDRKKEEATKTVFWNGMPIKS
                     NLITLCEQLGSMGVWVRLHYVYPYPHVDDLIPLMAAGKILPYLDIPLQHASPKILKAM
                     KRPGSIERTLERIKKWREICPELTLRSTFIVGFPGETEEDFQLLLDFLKEAQLDRVGC
                     FKFSPVEGAVATDMPDQVPEEVKEERFHRFMQLQQEISAARLQAKIGKTVQVIIDDID
                     EEGIIGRSMADAPEIDGVVYVDNPSNQLVTVGQIISVTITHADEYDLWGILNN"
     gene            complement(13960..14652)
                     /gene="mtnN"
                     /locus_tag="PXD66_000093"
     CDS             complement(13960..14652)
                     /gene="mtnN"
                     /locus_tag="PXD66_000093"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDL6318435.1"
                     /translation="MKIGIIGAMAQEIEILRQVMVEPKVTELAGCKIFEGKINNTRVA
                     LLQSGIGKVAAAIGTTLLIQLTQPDMIINTGSAGGLDPDLNVGDILISTEVRYHDVDV
                     TAFGYEIGQLPANPPTFSPNEQLVELAQKEAKKAGYNVVSGLICSGDAFINGNDKINQ
                     IRQNFPNVVAVEMEAAAIAQVCHGFNLPFVIVRAVSDVADKESHLSFEEFLPLAAEKS
                     STIVLAMLNNID"
     gene            complement(14710..14931)
                     /locus_tag="PXD66_000094"
     CDS             complement(14710..14931)
                     /locus_tag="PXD66_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HDL6318436.1"
                     /translation="MDNNYTLSAKITELETKVAFQELIIEELNQALIEQQFAIDKLQT
                     QIRHIAEKLKGAQLSQVASQAEETPPPHY"
     gene            15019..15744
                     /gene="fkpA"
                     /locus_tag="PXD66_000095"
     CDS             15019..15744
                     /gene="fkpA"
                     /locus_tag="PXD66_000095"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL6318437.1"
                     /translation="MLKTKLSLFTLIVGTLFSTNVMAEEKADPKFVDDSSYAVGVLMG
                     KNINGVIESQKDIFTYNQDKILAGVQDTLKKTGKLTDEDLQKQLKSLDAYLSAKEAKI
                     NEEKTKATVAAGDKFRADYKKQSGVKETASGLLYKIEKAGEGNSPKAEDTVKVHYKGT
                     LTDGTVFDSSYDRGEPIEFQLNQLIPGWIEAIPMLKKGGKMEIVVPPALAYGDRAAGK
                     IPANSTLKFEIELLDFKAADAKK"
     gene            15841..16497
                     /locus_tag="PXD66_000096"
     CDS             15841..16497
                     /locus_tag="PXD66_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL6318438.1"
                     /translation="MTKKFTPFSDEDHAILASYFPMVDGIAALIGEHCEIVLHSLEFL
                     EHSAIYIANGHNTNRKIGSPITDRALKSLHHMATDSVSKPYFTKAKGGVLMKSITIAI
                     RNGKQHVIGLLCININLEVPTSQFLNSFIAPVDTASEVTFASSVEDLVSQTIQTTIDD
                     VMADRQISNNNKNRQIVISLFEKGIFDIKDSINQVAEKLDISRHTVYLYIRQIKQDND
                     "
     gene            16497..16874
                     /gene="tusD"
                     /locus_tag="PXD66_000097"
     CDS             16497..16874
                     /gene="tusD"
                     /locus_tag="PXD66_000097"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HDL6318439.1"
                     /translation="MDYVLAIKSPVYGSQGAFLAYQVAEELLKANHTICQIFFFQEGV
                     SNANALVNPASDEVNLVEMWKKLAKLHRLPLHLCIAAAQRRGVVDEQISANATHNLAE
                     GFTLAGLGEFSQAVLKADRLLTF"
     gene            16876..17247
                     /gene="tusC"
                     /locus_tag="PXD66_000098"
     CDS             16876..17247
                     /gene="tusC"
                     /locus_tag="PXD66_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HDL6318440.1"
                     /translation="MKKYKLAFLFTQPPFGSAVSREGLDALLAASAFCDEQEIAILFI
                     NDGVFNLIADQQPEKLLQKDHISTFKLLELYDLTECFICQESVSLRGLDNADWLLRML
                     QFKTHAEMIALLNQAEKVMTF"
     gene            17257..17532
                     /gene="tusB"
                     /locus_tag="PXD66_000099"
     CDS             17257..17532
                     /gene="tusB"
                     /locus_tag="PXD66_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HDL6318441.1"
                     /translation="MLYTLSKANYDEETLNFFFSHLSENDAVVLWQDGVLQAVKSPQF
                     FAKLPRCYLLAQDVQARGLQEKLTAFQCISLSELVKLTAVYFPQVAL"
     gene            17648..18547
                     /locus_tag="PXD66_000100"
     CDS             17648..18547
                     /locus_tag="PXD66_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318442.1"
                     /translation="MVMAEENNTWVDKKHETVSTTLDRWANNINSWLGDADPDKPGTA
                     SLRVMLDSRWDKHDKFTYKPRVRGKIKLPILKKHLNVVFGDDELDNELTDKNHAGKLY
                     KNVNPNKRYNSREARDSNSSIGLRWSDGIKALGIETDLDAGIRSGSDIFGRLRISKDW
                     KITDQFSTRLEQIYRYGSESKHYLRTNFENKYTETDTTFIMNHTNFEYRHDVDEERSW
                     GNSLYRQHNFKPLTHISYGISMGGRLDKELSKFNYWGPFVSYRQPIWRNWLFIQPEIS
                     FYNDKDKDRKHFIQTFVRLEAIF"
     gene            18897..22922
                     /gene="rpoB"
                     /locus_tag="PXD66_000101"
     CDS             18897..22922
                     /gene="rpoB"
                     /locus_tag="PXD66_000101"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDL6318443.1"
                     /translation="MAYSYSEKKRIRKSFGKRPQVLNVPYLLTIQLDSFEKFIEKDPE
                     GQQGLEAAFRSVFPIVSNNGATELQYVSYELGEPVFDVRECQIRGTTYAAPLRVKLRL
                     VSYDREAAAGTVKDIKEQNVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFARIDRRRKLPATIILRALGYTTE
                     EILNLFFEKIKFEIADNKLLMTLVPERLRGETASFDIAANGKVYVEMGRRITARHIRA
                     LEKDNVTQIEVPVEYIVGKVAAQDYVDLSSGEVICPANMEISMEMLANLSQAGYNQIE
                     VLFTNDLDHGPYISETLRVDPTYDRLSALVEIYRMMRPGEPPTKEAAEALFDNMFFSA
                     ERYDLSAVGRMKFNRSLEIPEGVGSGILSNDDIIGVMKKLIEIRNGRGEVDDIDHLGN
                     RRIRSVGEMAENQFRIGLVRVERAVRERLSLGDLDGVTPQDLINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEVTHKRRISALGPGGLTRERAGFEVRDVHPTHYGRLCPIETPEG
                     PNIGLINSLSVYARTNNYGFLETPFRKVVNGQVTEEIEYLSAIEESNNIIAQANSNLD
                     ENFRFTDTYVTCRQQGESGLFKPEEVQYMDISTQQVVSVAAALIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMEKPIALDSGVAIVAKRGGTVQYVDASRIVVKVNEDE
                     TIPGEAGIDIYNLIKYTRSNQNTCINQVPCVNLGEPVARGEILADGPSTDLGELALGQ
                     NIRVAFMPWNGYNFEDSMLVSERVVQEDRFTTIHIQELSCVARDTKLGAEEITADIPN
                     VGESALSKLDESGIVYIGAEVKGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGTSGTVIDVQVFTRDGVEKDKRAKEIEEMQLCEAKKDLAEELEILEAGLF
                     TRVRNLLIEGGVKESALDGVAREKWLEQTLEDEAKQNQLEQLAEQHEELRKEFERKLE
                     IKRNKIIQGDDLAPGVLKVVKVYLAVKRQIQPGDKMAGRHGNKGVISKINPVEDMPYD
                     ENGQPVEIVLNPLGVPSRMNIGQILETHLGLAAHGIGDQIDKMIKQQQEVAKLREYMQ
                     KAYDLGHGSQVVDLSTFSDEEVMRLAQNLRKGLPLATPVFDGAHESEIKGLLELGGLP
                     TSGQITLYDGRTGEKFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGKA
                     QFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGTHYMEPGIPE
                     SFNVITKEIRALAIDMELDEA"
     gene            23185..27468
                     /gene="rpoC"
                     /locus_tag="PXD66_000102"
     CDS             23185..27468
                     /gene="rpoC"
                     /locus_tag="PXD66_000102"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDL6318444.1"
                     /translation="MKDLVKFLKAQTKSNEDFDVIKIGLASPDKIRSWSFGEVKKPET
                     INYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRER
                     MGHIELACPVAHIWFLKSLPSRIGLILDMPLRDIERVLYFESYVVTEPGMTDLEKNQL
                     LTEEQFMDAEERWGDEFEAKMGAEGIQALLRDLDLEHQCETLREELQETNSETKRKKI
                     TKRLKTLEAFLQSGNKPEWMVMTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINR
                     NNRLKRLLDLVAPDIIVRNEKRMLQESVDALLDNGRRGRAITGSNKRPLKSLADMIKG
                     KQGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFRPFIYSKLENRGIA
                     STIKAAKKMVEREDPIVWDILAEVIREHPILLNRAPTLHRLGIQAFEPLLIEGKAIQL
                     HPLVCAAFNADFDGDQMAVHVPLTLEAQLEARALMMSTNNVLSPASGDPIIVPSQDVV
                     LGLYYMTREKVNGKGEGMYFLDPREAEKAYRTGQAELHSRVKVRVTEYEKNEAGELEA
                     KTSLVDTTIGRSILWMIAPKGMPFSLFNQTLGKKAISKLINESYRRLGLKESVVLADQ
                     IMYTGFAYAARSGASVGIDDMVIPEQKYAIIEAAEAEVAEIQEQFNGGLVTAGERYNK
                     VIDIWAAANERVAKAMMENLSTEEVINREGNPEKQASFNSIFMMADSGARGSAAQIRQ
                     LAGMRGLMARPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLT
                     RRLVDVAQDLVIIEDDCGTHEGIVMTPLIEGGDVKEALRDRVLGRVVAEDVLKPGTDE
                     VLIPRNTLLDEKWCDVVDRESVDVIKVRSVVTCDTDFGVCAKCYGRDLARGHLINQGE
                     AVGVIAAQSIGEPGTQLTMRTFHIGGAASAAAKESSIQVKNAGTIKLTNAKFVTNKEG
                     KIVLTSRNTELTVIDTFGRTKENYKVPYGAVLSKHDGAEVAVGEVVANWDPHTMPVIS
                     EVSGRIQFSDIVDGLTVTRQTDELTGLSSIVVQDVGERATAGKDLRPALRLVDSNGKD
                     ILIPGTDVAAQYFLPGKAIVTLDDGAEIEVGEALARIPQESVGTKDITGGLPRVADLF
                     EARKPKEPAILAEISGIVSFGKETKGKRRLVITPAEGEAFEEMIPKWRQLNVFEGEMV
                     QRGDVISDGAETPHDILRLRGVHAVTDYIVNEVQEVYRLQGVKINDKHIEVIVRQMLR
                     KAVITNAYDSEFLEGEQVEVARVKIANRKRAEEGKPLVEFERELLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKQDELRGLKENVIVGRLIPAGTGFAYHQARAKKRAQLEA
                     GIAVVPEFEAPAAKANSFVTDADIEAEFEFVADDATQSLAALLNAGEEE"
BASE COUNT         8083 a         5522 c         6224 g         7703 t
ORIGIN      
        1 aaagaattat ctcgtagaat attaaaaagt ttagaacaaa aaaatattct cacttggcac
       61 ggcttaagtc caaaagatcc aacccaatac tacacactca agagatagta ggaaatataa
      121 gatgaataat acagttctca tcaccggctc aagccgtggt atcggtaaag cgattgcttt
      181 aacccttgca caacaagggt ttgatattgt ggtacattgc cgttctcgtg tggaagaagc
      241 ggaagcggtg gcacaagcgg tcagaaatct cggaaaaaat gccagagtgt tacagtttga
      301 tgtggcggat cgtgaaaaag tgcggtcgat tttagaggca gatgtggaac aacatggtgc
      361 ctattacggc gtggtgctaa atgcagggct tacccgtgat aacgcctttc ctgctttaac
      421 cgatgaggat tgggatagcg tgttgcgtac caatttagac ggtttttata atgtcctgca
      481 cccgattatg atgccgatga tccgtcgccg taaagcaggc aggattgtct gtatcacctc
      541 ggtttccggc ttgatcggca accgtgggca ggtcaattac agcgcttcaa aagccggcat
      601 tattggtgcg gcaaaagcac tcgccatcga gcttgccaaa cgtaaaatca cggtaaactg
      661 cgtggcgccc ggcttgattg ataccgatat tctggacgaa aacgtcccaa tcgaagaaat
      721 tctcaaaatg atccccgcag gacgaatggg cgatcctgat gaagtggcac acgccgttgc
      781 gtttttaatg gatgaaaaag ccacttacat cacacgccaa gtcatcgctg tgaatggtgg
      841 gttgtgttaa ctttaggata ataattcgta ggggcggatc cagtacccgc ccgaagccaa
      901 aaagagataa atatgaaacg agtcgtcatc accggaattg gtgtggttac cgcttttggt
      961 aaagagtggg cggaaattca cggctatcaa ccgccgaaac attggaaccg caagcagttg
     1021 cgcagccttg ggcgagtgtc gcaatttgtg gtggaggcca gcgagcgggc tttagcacag
     1081 gccggtttgt tggacgaaaa cggcgtgatt ttcgactggc taaaagacag gcgaatgggc
     1141 gtggcgtgtg gctcgtccac aggctctacc aatgacatca aagatgccgc cgaattgctg
     1201 atgaccggca aatcggacgg tttcaatgcc aacacctatg tgcgaatgat gccccacacc
     1261 accgctgcca atattggcat tttcttcggg ctgacagggc ggattatccc gacttccagc
     1321 gcttgctcct cgggcagcca aggtatcggt tacgcttacg aagccattaa atacggtatg
     1381 atcccgatga tgcttgccgg cggtggagaa gaattttgtg cctccaaagt ctatgtcttc
     1441 gacagcctct atgctgccag ccgccgcaac gacaacccaa aaggcacgcc acgcccctat
     1501 gacaaagacc gagacggctt agtgatcggc gagggagcgg gaatgttcgt attggaagag
     1561 ctggaacacg cgttagcacg aggggcaaac attatcgccg aagtggtggg ctacggtgca
     1621 aacagtgacg gttcccacgt cacccgccca caagccacca caatgcaacg ctgtatggaa
     1681 cttgccctta aagatgccgg tattcaaccg aaccaagtcg gctatgtaaa cggacacggc
     1741 acggccaccg agcagggcga cattgccgaa actcaagcca ccacagcggt gttcggcaaa
     1801 attccgctca gctcgcaaaa aagctactta gggcatacgc tcggtgcgtg tggtgcattg
     1861 gaaagctggt tcagcattga aatgatgcga gacggctggt tcgccccaac actgaattta
     1921 gaaaatgtcg atgaacgctg tggcgacttg gattaccttc aaggtgacgg acgggaaatc
     1981 cacaccgatt atgtgatgaa caacaatttc gccttcggcg gtgtgaatac ctcattgatt
     2041 tttaaacgtt ggaaataacc gttttcaaat aatgaaaaag atattattag tttattactc
     2101 ccaaaccggg caactcaccc atttggcaga aaattttgta caatcgcttg aacaagcggg
     2161 cgtttttgtg gaaaaactcg caatcaaacc gcaacaggaa tacccgtttc cgtggcggtt
     2221 tatgcgtttt tttaacacct tccccgaaac cgtccacctc acaccgccgc cgattgagcc
     2281 tctgcccttt cagcacgaaa tatacgattt ggtgatcatc gcctattccg tgtggtttct
     2341 ctcccccagc cagccgatta ccgctttttt acagagtgaa caggcaaaaa agtgttgaag
     2401 gatacgcccg tcatcacgct gatcggctgt cgcaatatgt ggctgatggc gcaggaaaaa
     2461 gtgaaaaaac tgcttgccga gagtgaggcg aagctcattg gcaacgtggt caaagtggat
     2521 caatctaacg actgggcgag ttttatcacc acaccgcttt ggatgctgac cggcaagaaa
     2581 aaaaatcggc aagctgccga gcgctggcat tgccgaaagc gaaatggaag atgtaaaacg
     2641 cttcggggca aaacttttgc acatttttag ccaaaaccaa ccgcttgaca gcaccctttt
     2701 ccgccaaatg caagcggtca aaattgacga aaaattgatg atgagcgaaa aagtcggtgc
     2761 gagaagtttc cacctttggg gcaaattact gatcaagtgc ggtcaaattt caccgcactt
     2821 acgccaagcg gtactctatt tttacatcct ctttttagtg acgttaattt taaccgtcgt
     2881 cccgatttca acggtgatta aacggctatt acgcccactg ctgaaagaaa aattagaaaa
     2941 acaaaaacgg tattttgccg agccttcggg cgagtaaacg taccctacac ggaaacttta
     3001 tgctatctaa cgtctatatc accaacacct ccgccttttt acccaacgcc cctgtgtcca
     3061 acgatgcaat ggaagcggtg ttgggaatgg tgggggaaac gccttcccga gtgcggaaga
     3121 tgattttgcg ttctaacggt attcaacatc gttattatgc cattgacccg ataacccgcc
     3181 aagcgactca cagcagtacc gagttggcgg tgcaagcggt caaaaaattg ggaaattctg
     3241 caacatcagc gacttgcttg gcggtgggga catcttaccc cgaccaaatt atgccggggc
     3301 agggggtgat ggtacacggc ttgcttgatg ccgaaccgat ggaagtgctg accactgccg
     3361 gtgtgtgcgt ggcgggaatg gcagcgatga agcacgccta taatgccgta cgcatcggtg
     3421 agcatcgtgc tgcgattgca acggcttccg aaaccgcctc cgccattatg cgcagtgaac
     3481 atttccgtgc cgaaatcgat cataaaaaac tggacgatgc tcgccctgag atcggttttg
     3541 agaaagattt cctgcgttgg atgctctcag acggtgcagg ggcggtattg cttgccaatc
     3601 agccgaacga aaaagggttg agcctcaaaa ttcactggat cgatctggtc tcttacgcta
     3661 acgaaatgcc ggtttgtatg tacgccggtg cggaatttat tgacggcaac tacaccggct
     3721 ggaaagcaat gagcgaagcc caacgtgccg aatgctcggc aatggcggtg aaacaagatg
     3781 tcaaactgct caacgaaaac atcgtcaaat acacggtgga aaaagcattg gagcggatta
     3841 tcccgaaata caagctcaac gctgaagaga ttgactactt cctgccgcac tactcgtccg
     3901 gttccttccg agataaatta ttggaaggtt tacagcggat caatttcctg atcccacagg
     3961 aaaaatggtt caccaacctg actaccaaag gcaacactgg gtcagcctcg atctacatta
     4021 tgctcgatga atttatccgc acccacgatc tcaaacaggg acaaaaaatc ctctgctatg
     4081 tgccggaaag cggacgtttt tcgtcttgtt ttatgctttt ggaagcggtg gtaaattcgt
     4141 aaaatcgtag ggacgggggc tattcccgtc caacaatcat cggaaaacgt atggacatca
     4201 atgacattcc ccaagacaac agcaaaatct tcaacggcga acgcaaagtg atctacgccc
     4261 gcaatccgca accagaaatc cgccaaatga tctcctctat tctcgcttgc tacgcttggg
     4321 acgaaaacaa cccgtttgta gcgaagtctg atgcacggtt gaaggcattg gcggaagttg
     4381 ttggtaaagc agaaattggc gattaagttt gattttgtgc tttacagggt cgcagattat
     4441 ctgtataatt tgcgacctta acttgcgaga gcaagctgat ggtgcttagc ctatctaagc
     4501 cccgtctaag actgtagggg tgaaaactta ataatcctac atagatggtg aacagacaga
     4561 attctctgct tctggacacc ttaggctcaa agaagattgc agtttgtagc aatgcaagcg
     4621 gtaatatttt tgggattttt tgtaaattct gcttgaaaag gcagagtgta tcaggagcta
     4681 aaaccaatgg cattaaatct tcaagacaaa caagcgattg ttgctgaagt aaacgaagct
     4741 gccaaaggtg cactttcagc tgttgttgcg gattctcgcg gtgtaacagt tgaaaaaatg
     4801 actgagttac gtaaagcagc tcgtgaagct ggtgtgacaa tgcaagttgt acgtaatact
     4861 ttattacgtc gtgcagtgga aggcaccgag ttcgagtgct taacagatac gtttaccggt
     4921 ccaactctta tcgcattctc aaatgaacac ccaggtgcag cagcacgttt attcactgaa
     4981 tttgcgaaaa caaacaaaga gtttgaactt aaaggtgcag cctttgaagg taaaaaacaa
     5041 gatgttgcat tcttagcaac cttaccaact tacgaagaag caattgcacg tttaatgggc
     5101 acaatgaaag aagctgcggc aggcaaactt gttcgcactc ttgcagcatt acgcgatcaa
     5161 atggaagcag cagcttaatt ttaagcactc ttctttattc gtttaacaac ttattttact
     5221 ttaggaatcg attgttatgt cattaactaa cgaacaaatc attgaagcga ttgcttctaa
     5281 atcagtatca gaaatcgttg aattaatcac tgcgatggaa gaaaaattcg gtgtttcagc
     5341 agcggcagca gtagcagcag ctccagcagc aggcgcagcg gcagcagaag agaaaactga
     5401 gtttgatgta gtattagcag aagctggtgc aaacaaagtt gctgtaatca aagcagtacg
     5461 tggtgcaaca ggtttaggct taaaagaagc taaagactta gtagaatcag ctccagctgc
     5521 attaaaagaa ggcatctcta aagcagaagc tgaagcactt aagaaagaat tagaagaagc
     5581 tggcgcgaaa gtagaaatca aataattttt gatttatttt gtcagaactt caagttcaac
     5641 aaaccccgat ggaaacatcg gggttttgtt ttttcagaat tgtgtaatgt agatatgatt
     5701 acctttatcc attacttgcg cagcagtgaa aaatgtaaaa attccacaaa gtgatctgcc
     5761 cagtgcattt cgtggtggat ttcatttgcc ataatccgca ggcggatatt gtctaacggc
     5821 acgccaatcc gaatcagtgc ttggtagtaa ttgagcgaac aatcaatata ggtttggttc
     5881 gtattagaga taaagtgcga atcgatttca tcgccttcgt tagtgccaac ctgaataaac
     5941 actttggtat tgggttgtaa cggatattgg tgcgtaaaac gcagaaaatc cggctcacta
     6001 aaccaagagg caagcgaaaa cacccctaaa tgcccgaaaa tatgtgggta gcggctgccc
     6061 atataagcgg tgataatgcc acccattgaa ctgcctgcca ataaggtatg ttcttgctga
     6121 ggcttggtgc gatagtgaat atcaataaag ggtttaaccg tttccaccac ccatttggca
     6181 tattcctccc ctaagcctcc ggcatttcgg gtttcagggg ttgagccggt ggttgttttc
     6241 cacgggccgt attcatttaa gcgattagct cctgcgttat caatgccgac aataatcatc
     6301 ttcggtaatt cttggtgtga tttaatggtt ggaataattt tccaagaatg ccccgaataa
     6361 gactctttgc tgtaaaacac attttgcccg tcgtgcatat ataacaccgg ataacgttcc
     6421 caaccttcgt tgtgataatc cttcggcaat agtacccgaa tgcggcggtg atggtgtaaa
     6481 tagggaacat ataaaaaatg ctcctgcatt tctaaatagt ggttaagtgg tttttgtacc
     6541 atcagtctgt ccttttgctt aaattgtggc gtattttaca ccttaatcag tctgagttaa
     6601 aacaagcggt cagaaaaagt caaaaatttg caaattgctg ttacctaacg caatcggttg
     6661 ccttatgctt ttgaggaata taaaagagaa actgtgatct aagtcacagc aatttttatt
     6721 cggtttgact aaaatccgaa ctatcactta atagagagga aaatgttatg caggcttcat
     6781 tacaagatat tcttgaggta gtaaaagcaa aaaatctcac ttatcagcaa aaattgatga
     6841 gtttaggcaa tattgctgag cggttattta acccgattga actacttggc tacactgaag
     6901 aagagtggga acacattgaa aatcagatga tttgcgattt aaatgaaggt tatgcgattt
     6961 atcgcccacg ttatattttg cctgattata atgtgtatat gcaaaaaggt tgccaattct
     7021 tagatttacc tccgccaacc aatttagatg aagccttaga cggcttgtta attctgtatt
     7081 cacacgttcc gtccattact acttttccgg tgtatatcgg gcgtttagac caactgttag
     7141 aaccgtttat aaccgatgaa gagcaagatt acattaaaat caagcgtttc ttaaatcata
     7201 tcgataaaac catttccgat tctttctgcc acgctaacat cgggccgaat gacaccaaag
     7261 ccggtcgttt aatcttaaaa gcggtgattg agcttgaaaa cattacaccg aatatgacga
     7321 ttcgttatga taaaaccaaa acatcgcgtg aatttgccga actggcggcg aaagcgtgct
     7381 tgttggtgtc caaaccgtct tttgctaatg atgcctacta cattcaagat ttaggcgaag
     7441 aatacggaat tgcaagctgt tataacgcct taccggaatg tggcggtgct tacactctga
     7501 cccgcctgcg tttaggtact atcgggcgtg cttgtgcgag tgttgaacaa atggtcaatg
     7561 agatgctgcc gaaagtggca aaactcgccc tttccacgat ggataaacgg cataaattct
     7621 tggtggaaga aagcaatttc tttgaaagta gcttcttggt tcaggagggc tttatcaaac
     7681 gtactaactt cacaagtatg attgcgattg taggtcttgc tgatgcggtg aatcatttac
     7741 tcaagcaaga aggtttaaat gaaacctttg ggcaaagcaa acgtggcgat gaaattgcga
     7801 cgctgattat ggataagctc caagcagtaa atgacgctca taaagggctg tatgttgaac
     7861 gaaccaataa ccaatatctg ttacacgctc aagtgggggc aaataaccac gctgaagaca
     7921 aaatgaacac gccggcacac cgcattcgtg tgggcgaaga gccaacctta ttggcacact
     7981 taaaacagtc tgctcctttc cataaatatt tcccatccgg tacgggggat ttattcgcct
     8041 ttgaccaaac ctacactgat catttagatg cggtggtgga tattattgac ggctcgttcg
     8101 cccaaggtta tcgctacatt accacttacc tgaaaaatac tgatttgatt cgagtgacag
     8161 gctacttggt gaaaaaaagc gaggttgaac gttatcgcaa aggcgaagcg gtattgcgtg
     8221 ataccacttg gtatggttca ggcaccgatg attgtgcgaa agtgttcgac cgccagctgc
     8281 gtgatgaaga aaatgtgaat gcagggtaac aatggctgca ctttctgaga tttttgtgcc
     8341 gttacaccgc attatcccct tctcgaatgt agaagggcag ggcaatcgta ccagcatttt
     8401 tctacaaggt tgcaagctga attgcctgta ttgccataac ccggaaacca ttccacgtta
     8461 tagcaaggaa gcaaaacagg ttagcttgca atacttgtac gatcaagtga tggaggcagt
     8521 tccctttatt cgaggagtca ctatttcggg tggagagcca accattcatc ataaaaaatt
     8581 agtgccactg ttcgaggcgt tgcgtgagca agggctaacc tgttatttag acagcagcgg
     8641 cttttttgat tttgaggcaa cagagccgtt aattaatgtt accgataaat ttctgttcga
     8701 tctcaaagga gatggggcgg gcttacaaac gctatgcttt gatcggaaaa accaagccgg
     8761 caaagtgcct gaacaggctc ttccaaacgt gaatcatatt aaacctgaaa acttggctcg
     8821 caatttgcaa aatttagcca aattattacc gcttgataag gtggaagaag tacgtttggt
     8881 ttacctcaat gagttttata acgcccacca tttgctcgaa aaagtggctg acctacttaa
     8941 accttaccca gatgtactac ttaaaattat tcgggtacac accaaaggag cgagagatga
     9001 aacaggcttg gctcgttttg tacccactat cgagcaaacc gatgaactgg cagcatttgc
     9061 aaaacggcac ggtattcaaa aagttattac tatttactaa tggagactta cgatggatat
     9121 actaattagc ataattggtg tttttgtgtt attaggctta ggtgttttat tatcaaataa
     9181 tcgcaaagcc attaaatttc gcacaatttt gggcgctttg gcgattcaaa ttggttttgc
     9241 cgctcttatt ctttactttc cagcgggaag aaatgcattg ctagctactg caaattgtgt
     9301 gagcaatatt attaattatg gtaacgaagg tattagcttt gtgtttggca atttagcgaa
     9361 tccgagtaat agcagcattg gttttgtgtt tgcggttaaa gtattgccta ttattatctt
     9421 cttctctgcg ttaatttcaa tgctttacta ccttggtgtg atgcaatggg tgattaaaat
     9481 tattggtgga gcattacaaa aaacactagg cacttccaag gcagaatcaa tgtcagcggc
     9541 agctaattat ttttgttggt cagacagaag ctcctctagt tgtaaaacca tacattagcc
     9601 gtatgacaga gtcagaatta tttgccatta tggttggtgg aactgcttca attgctggtt
     9661 ccgttatggc tggttatgcc ggaatgggcg ttcctttaac ttatcttatt gcggcctcct
     9721 ttatggctgc accagctggg ttactatttg ctaaattaat gtatcctcaa acggaaacct
     9781 tggagagtaa agcagatgac aatgttgaag ttgaaaagcc aaataacgtg gttgaggccc
     9841 tagctaatgg tgctagtgca ggtgttactt tggctattaa tgtcggtgct atgttaatag
     9901 catttattgc agtgatcgct ttattaaatg gctttattgg tggcattggt aattggtttg
     9961 gttatgaggg gctaacctta caaattttat taggttattt attccaacca cttgctttct
    10021 taatcggtgt gccgtggcaa gatgccgata ttgcaggtca aatgataggt atgaaacttg
    10081 ctgttaatga atttgtcgga tatcttgagt ttgctaaata tctacagcct gatgctgtgg
    10141 tgcaattaag cgataaaact aaggcaataa ttacttttgc tttatgtggt tttgcaaact
    10201 ttagagcaat tgcggtattg attagtggta tcggaagtat ggcaccaagt cggcgttcag
    10261 atattgctcg tttaggttta aaagcagtga ttggcggcac attggcgaac ttaatgagtg
    10321 caactatagc gggtttcttt attggtttaa gtggtgccac attaggttga tacaagcggt
    10381 taaattgact cacttttttg caaaaagccc aagtaagtta aatgactact tgggctttgt
    10441 ttaatgaact agctcttagt ttcggttttt gctgtgttat ccttttccgc tttggctgtc
    10501 tgtgttttcg aagttgtctg tttttttgcg gattggctag cagtagctgc ttttcttact
    10561 ttctttgttt taacttctac ttccgcattt ttagagtttt tatccgctaa ccatgcctgc
    10621 actttagcta agaatatttg tcccatcggg cgaggatcgc cagaaaataa tgtttgtaat
    10681 ttattatttt caataatgtc gcgtctaatt gctaagcgtt cttcgtggtt ttccgctaaa
    10741 cgaatagctt gagtaacgta ttcctctgct gtttgggtaa ttaaccaatt aggtagcccg
    10801 aggcgcttaa ataaaccttc atcaatatgt tcatgaactt cagggcctgt tttacagaca
    10861 ccaactaaac caagcgtaac catatcgata atgccgtttg tatttccaaa tgggaatgga
    10921 tttagcatca tatcgcaatt atgtagaata tttagatatt catcatatgg cgaatgtggg
    10981 tgtgcggttg catcatttcc taaatagctt ttgataaaac gttccacata aggatgcgta
    11041 attccactag attgccctaa tgcaaaatgg aaatgagttt ttaccttcgc cctatcacga
    11101 atggctttta atgcctctaa gaaatatggg tttaatttca tagtggttga tgcaattccg
    11161 atatggataa cctctggatt ttcacgtagg ttatagacca cattttgagg cgctaatgcg
    11221 gaaggcacat agggtagcgc atcttttgga agacgtaata attgttcact aaagcagctt
    11281 tcggaaccaa catagtcatc ttctacgatg acatattcaa taaaatcaga gtgtgtagtt
    11341 gccggatgac ctagcgcaac agcttgaatt ggtgctaggc gagtgttact agcgaaaata
    11401 gtggttaaat ccattccaat gctaggcata tagaatacgg ctggattatt tttatcacag
    11461 atatctttta aaaagcttaa gcggtcaaat agtgagccat cttgtggtaa taaatgaaat
    11521 tcgtcgaata cctgttgccc catttcatct acagcatcac tgcctagccc aataagatgg
    11581 aaatgttcgc gtgcagcaac cattgaggtt gaatgagtac gataaataga atgtgatgaa
    11641 tggaaatgct ccaataatac taccatgaca ggtttattat ttctggtacc aagctgttca
    11701 atttttctat cttcccaacc gacagaaagt aaatgacgac gaattacagc atttaacgac
    11761 cgttttacat tatgtttatt tgcttcaata tcataactgc aatgcatata aacatcgtgt
    11821 gaaatggcgc tcggtagctt gttcaagttt tcaatttgcg ctaatttttc tgggaaccat
    11881 tgcaaaatag ccgcgcgttt tgcaaaagca gaggatgtac caataaatct aggtgattgg
    11941 agtgcaaaac aaagtgatgc ggtgagatct gatgaagcat tccatgcagc atctagattc
    12001 aataaaatat tagattccgg taagtataaa atacagaact taattagtgc ttgtaatgtg
    12061 gcatctaaat caaccgtatt tggattagca gattctttat ttctattgta agtacgcaag
    12121 atatggtctg cgtttacgta tggagaactg gcaaaaatta atgctatcca gcgttgtaat
    12181 gtcagaaaac gcatagcacc gtgttcggag atctctaatg caggatcttc aaacaagcga
    12241 gtaatcgcta ccgccattcg agtacagaaa tagatagctt tatcattttc taaattttca
    12301 atctgctctg gcatattcaa ttcaatattg gaaatgccac caaaattact atctaatttt
    12361 gaaaggatag acagtagctc ggtacaagcc gattcataat cttttttcgc gaccgcctgc
    12421 tcaaaacgga tgacgctagg catattttct gctgacataa tgtatcctta gttgtttaaa
    12481 attccccaca gatcatactc atctgcatgt gtaatggtta ctgaaataat ttgccctact
    12541 gtcactaatt ggtttgaagg attatctaca tagactacac cgtcaatttc cggtgcatct
    12601 gccattgagc gaccgataat gccttcttcg tcaatgtcat caataattac ttgtacggtt
    12661 ttgccgattt ttgcttgtaa gcgagcagcg gaaatttctt gttgtagttg cataaaacgg
    12721 tggaaacgtt cctcttttac ttcttccgga acttgatccg gcatatcggt agcaacagca
    12781 ccttccactg ggctgaattt aaagcaacca acacgatcta attgtgcttc ttttaagaaa
    12841 tcgagtaata gttggaaatc ttcttctgtt tcccccggga agccgacaat aaaggtagaa
    12901 cgcagagtta attcagggca aatttcacgc cattttttaa tgcgttctaa ggttctttcg
    12961 attgaacccg ggcgtttcat cgcttttaag attttcgggc tggcgtgttg gagtggaata
    13021 tccaaatacg gcaaaatttt gccggcagcc ataagcggaa ttaaatcatc tacgtgtggg
    13081 tatgggtaaa catagtgcaa acgcacccac acgcccattg agcctaattg ctcacatagc
    13141 gtaattaaat tacttttaat cggcataccg ttccagaaaa cggttttggt tgcttcttct
    13201 tttttgcgat ctaacgaata ggctgaggtg tcttgtgaga cgattaacag ctcttttacc
    13261 ccggcatcaa ctaggcgttt tgcttcatct aatacttgca cgatagaacg gcttgctaaa
    13321 tcgccacgca ttgaaggaat aatgcagaaa gtgcagcgat gatcgcagcc ttcggaaatt
    13381 ttcaaataag cgtaatgctt cggtgttaat ttaatccctt gttttggcac taagctggtg
    13441 taagggttaa attccggttt tggcacatat ttatggacgt ggttcattac cgcctcatag
    13501 ctatgtggcc ctgtaatttc caatacttta gggtgtacct cacgaatttg gttttctttt
    13561 gcccctaaac agccggtgac aattactttt ccgttagctt ctaacgcttc accaatgctt
    13621 tcaagagatt cttgtactgc ggaatcaata aaaccgcagg tgtttacaat cacaagatcc
    13681 gcattttcat aactagggat gatgttataa ccatccgcac gcagttcggt caaaatacgt
    13741 tcggaatcaa ctaaattttt agggcagcct aagctgataa aaccaatatt tggtgaagca
    13801 ctcatataac tctcaataac taaaaagaaa aatgcctaca atttttgtct gtaggcaaaa
    13861 aactaacaag attctacaaa atcttgccca aaatagcgat aacaagcggt caatttttaa
    13921 taaacattta caaacttgaa atttcgctaa caattaaaat taatcgatat tatttagcat
    13981 tgctaataca atcgtggaag atttttctgc ggctaatggc agaaattctt caaaagaaag
    14041 gtgggattct ttgtccgcta catccgatac tgctcttacg attacaaatg gaaggttaaa
    14101 accgtgacaa acctgcgcaa tggctgctgc ttccatttcg actgcaacga cattcgggaa
    14161 attctgacga atttgattaa tcttatcatt accgttaata aaggcatcgc cgctacagat
    14221 taagcctgaa accacattgt aaccggcttt tttcgcttcc ttttgagcta attcgactaa
    14281 ttgctcgttt ggcgaaaatg ttggcgggtt tgccggtagt tgcccgattt cataaccaaa
    14341 ggcggtcaca tcaacatcgt gataacgaac ttcggttgaa atcaaaatat cacctacgtt
    14401 taaatctgga tctaatccac cggcagagcc tgtattaata atcatatccg gctgggtgag
    14461 ctgtatcagt aaggtagtgc cgatggcggc agcaactttg ccgatacccg attgtaataa
    14521 tgccacacgg gtattgttaa ttttgccttc aaaaatttta caacctgcta attcagtgac
    14581 tttaggttca accattactt ggcgtaaaat ctcaatttct tgtgccatcg caccaataat
    14641 accaattttc attactttcc ttctctaatg tattgtttat tttttgaaaa aaacgctatt
    14701 ttaacagcat taatagtgag gagggggtgt ttcttctgct tgtgaagcaa cttggcttag
    14761 ttgagcccct ttcaatttct cagcaatatg gcgaatctga gtttgtaact tatcaattgc
    14821 aaattgttgc tcaataagtg cttgatttaa ctcttcaata atcagctctt ggaaagcaac
    14881 tttagtctcc aattcggtaa tttttgccga taatgtataa ttattatcca ttttttacta
    14941 aaaaaaggtt gtgtgaaaag gaattacact ttaccctatc ttaaattttc ttaacagtta
    15001 tttttataag gaaaaattat gttaaaaacc aaattatcat tatttactct tattgttggc
    15061 acacttttta gtacaaatgt gatggcagaa gaaaaagcag atcctaaatt tgtggatgat
    15121 tcctcttatg ccgtgggtgt attaatgggt aaaaatatta atggtgtgat tgagtcacaa
    15181 aaggatattt ttacctataa ccaggataaa atcttagccg gtgttcagga caccttaaag
    15241 aaaaccggta aattaaccga tgaagatttg caaaagcagt taaaatcgtt agatgcctat
    15301 ttatctgcga aagaagcaaa gattaacgaa gaaaaaacca aagcaacggt agctgcaggc
    15361 gataaattcc gtgctgatta taaaaaacag tctggcgtaa aagaaacggc ttcaggcctg
    15421 ttatataaaa ttgaaaaagc aggtgaaggc aattcaccaa aagcggaaga tactgtgaag
    15481 gtgcattaca aaggcacttt gactgatggc acggtatttg acagctctta cgaccgtggt
    15541 gagccgattg aatttcagtt aaatcaatta attccgggtt ggattgaagc gattcctatg
    15601 ttgaaaaaag gcggtaagat ggaaattgta gtgccacctg cattagctta tggtgatcgt
    15661 gccgcaggca aaattccagc aaattcaacc ttgaaatttg aaattgaatt attagatttt
    15721 aaagcagcag atgctaagaa ataattcgtt attccttaat tattgaagat acgtaagcgg
    15781 ttagatttat tataatattt gcaaaaaatg tgataaaact aaccgcttgt aatttgtatt
    15841 atgaccaaaa aatttacgcc tttttctgac gaagatcacg ccattcttgc ttcttacttt
    15901 ccgatggtag atgggattgc ggcactgatt ggtgagcatt gcgaaattgt attgcattcg
    15961 ttagagtttc tggaacattc ggcaatttat attgctaacg gacacaatac taaccgtaaa
    16021 atcggctctc cgattactga ccgtgcattg aaatcgctgc atcatatggc aacggatagc
    16081 gtctccaaac cttattttac caaggcaaaa ggtggcgttt taatgaagtc tatcactatt
    16141 gcgattcgta atggtaaaca gcacgttatc gggttactgt gcattaatat taatttggaa
    16201 gtgcctacct cacagtttct caattctttt attgcccctg tagataccgc ttcagaagtt
    16261 acttttgcca gttcagtaga agatttggtt tcacaaacga ttcaaacgac cattgatgat
    16321 gtgatggcag atcgtcaaat ttctaacaat aataaaaatc gtcaaattgt gatttcgtta
    16381 tttgagaaag ggatttttga tattaaagat tccattaacc aagtagcgga gaagctggat
    16441 atttcacgcc atactgtgta tctctatatt cgtcaaatta agcaggacaa tgattaatgg
    16501 attatgtatt agcgataaaa tcaccggttt atggttcaca aggggcattt ttagcctacc
    16561 aagtggcgga agaattatta aaagccaatc atacaatttg ccaaatattc ttttttcaag
    16621 aaggcgtgag caatgctaat gccttagtga atccagcaag cgatgaagtg aatttagtcg
    16681 agatgtggaa aaaattggct aaattgcacc gcttgcctct gcatttgtgc attgctgctg
    16741 cccagcgtag aggcgtggtg gatgagcaaa tctcagctaa tgcaacacat aatttagccg
    16801 aaggatttac cctagccggg ttaggcgaat ttagccaagc agtattaaaa gcggatcgat
    16861 tactgacttt ttaagatgaa gaaatataaa cttgcctttt tatttaccca gccacctttt
    16921 ggttcggcag ttagccgtga ggggctagat gccttgcttg cagctagtgc tttttgtgac
    16981 gagcaagaga tagctatcct ctttatcaat gatggcgtat ttaatctcat tgccgatcaa
    17041 cagccggaaa aactcttaca aaaagatcat atttccacct tcaaattatt ggagttgtat
    17101 gacttaactg aatgttttat ttgccaagaa agtgtaagtt tacgagggct ggataatgca
    17161 gattggttac tgcgaatgct tcaatttaaa actcacgctg agatgatcgc attgctcaat
    17221 caagctgaaa aagtaatgac gttttaagga atatttatgc tttataccct ttccaaagcg
    17281 aattatgatg aagaaacctt aaacttcttt ttctcacatc ttagtgagaa cgatgcggtt
    17341 gttctgtggc aagatggcgt gctacaagcg gtcaaaagtc cgcaattttt tgcaaaattg
    17401 ccccgttgct atttattggc acaagatgtg caagctcgag gattgcaaga gaagctcact
    17461 gcatttcagt gcatttcatt atccgaatta gtcaaattaa cagcggttta ttttccccag
    17521 gttgcactgt aacctagaga ggatattttt tgtaatacca aataatttag attatgatgt
    17581 gattcctaaa aaggaaaata ttatgaaaac aaaatgtctt attccgctac tagcacttac
    17641 acctgttatg gtgatggcag aagaaaataa cacttgggta gacaagaaac atgaaacagt
    17701 aagcactacc ttagatcgtt gggcaaacaa tattaacagt tggctaggtg atgcagaccc
    17761 agataaaccg ggaacggctt ctttacgggt aatgttagat agccgttggg ataaacacga
    17821 taaatttact tataagcccc gtgttcgtgg taaaatcaaa ctccctatct tgaaaaagca
    17881 tctaaatgtc gtatttggtg atgacgaact ggataatgag cttaccgata aaaatcatgc
    17941 tggaaaattg tataaaaacg tcaatccaaa taaacgctat aactctcgtg aagcgagaga
    18001 ttcgaactct tctatcggtt tacgttggtc tgatggcatt aaggctttag ggattgagac
    18061 cgatttagat gctggtattc gttcgggaag cgatattttc gggcgtttac gcatcagtaa
    18121 agattggaaa atcaccgacc agttcagtac ccgcctagag caaatttatc gttacggttc
    18181 agagagcaag cactatttac gtactaactt tgaaaataaa tataccgaaa cggataccac
    18241 ctttattatg aaccacacca attttgaata tcgtcatgat gtagatgaag aaagaagttg
    18301 ggggaatagt ttatatcgtc aacataactt caaaccactc acgcacatca gctatggtat
    18361 cagtatgggg gggcgtttag ataaagagtt atcaaaattt aactattggg gtccatttgt
    18421 ctcctatcgt caaccaatct ggcgcaactg gctctttatc cagcctgaaa tcagttttta
    18481 taacgacaaa gataaagatc gtaagcactt tattcagaca tttgtacgtt tagaggctat
    18541 cttctagcta ttaagcctca agtagaatct tggggcttgt ttttgcaagc ggtcaatttc
    18601 ttgccaaaat ttacaaaatc cgctataatt cctaaccatt tttagcaaaa attggctgac
    18661 ggcttgaccg gtcagccttt tgtgcttgaa cgcaagggct gattttgtca ttttaatcag
    18721 caaaatttgc aaatttcctg cccaatttga ccgcttgcag tagcgatttc tgactgcttg
    18781 attggcgatt ggaaaatgac atctcgtaca gtttttataa tgccacttcc aaccgagtct
    18841 ttcgtgtcgg attgtgtaat ctcaaccaaa atatatttca gaggctatct taaaaaatgg
    18901 catattcata ttccgagaaa aagcgtatcc gtaagagctt tggcaaacgc ccacaagttc
    18961 tgaacgtacc ttatctatta acaattcagc ttgattcttt tgaaaaattt atcgaaaaag
    19021 atcctgaagg gcagcaaggt ttagaagcgg cattccgttc tgttttcccg attgtgagca
    19081 acaatggtgc aacagaatta caatacgttt cttacgaatt aggcgagccg gtgtttgacg
    19141 tgcgtgagtg ccaaattcgt ggtacaacct acgctgcacc attacgcgtg aaattgcgtt
    19201 tagtctctta cgatcgtgaa gccgcagccg gtacggtaaa agacattaaa gagcaaaacg
    19261 tgtatatggg cgaaatccca ttaatgaccg ataacggtac tttcgtgatt aacggcaccg
    19321 agcgtgttat cgtttctcaa ttacaccgta gcccaggtgt gttcttcgat tctgacaaag
    19381 gtaaaaccca ctcttcgggt aaagtgcttt ataacgcacg cattatccct taccgtggtt
    19441 cttggttaga tttcgagttt gatccgaaag ataacttatt tgcccgtatc gaccgccgtc
    19501 gtaaattgcc ggcaaccatt attctacgtg cattaggcta caccaccgaa gaaatcttaa
    19561 atttattctt cgagaaaatt aaatttgaaa ttgccgataa taaattattg atgacgttag
    19621 tgcctgagcg tttaagaggc gagactgcat catttgatat tgcagcaaac ggcaaagtat
    19681 atgttgaaat gggtcgccgt attaccgctc gtcatatccg tgcgttagaa aaagacaatg
    19741 tgacccaaat tgaagtgccg gtggaatata ttgtcggcaa agtagcggct caagattacg
    19801 ttgatttatc ttcaggtgaa gtgatttgcc cggcgaatat ggaaatctcg atggagatgt
    19861 tggcgaattt atcacaagcc ggttacaacc agatcgaagt attatttacc aacgatttag
    19921 accacggtcc atatatttct gaaaccttac gtgttgatcc aacttacgac cgtttaagtg
    19981 cgttggttga aatctatcgt atgatgcgtc cgggcgaacc accaacgaaa gaagcggcag
    20041 aagcattatt cgataatatg ttcttctcgg cagagcgtta tgatttatcg gcagtaggtc
    20101 gtatgaaatt caatcgctcg ttagaaattc cggaaggcgt aggcagcggt attttaagca
    20161 acgatgacat catcggtgtg atgaaaaaat taatcgaaat ccgtaacggt cgtggtgaag
    20221 tggatgatat cgaccactta ggtaaccgtc gtatccgcag cgttggtgaa atggcagaaa
    20281 accaattccg cattggtttg gtgcgtgtag aacgtgcggt gcgtgagcgt ttatctttag
    20341 gcgatttaga tggtgtaaca ccacaagatt taatcaacgc taaaccgatt tcagcagcag
    20401 tgaaagagtt ctttggttca tcacaacttt cgcaatttat ggaccaaaac aacccgcttt
    20461 cagaggtaac acataaacgc cgtatctcgg cattaggccc gggtggttta acccgtgagc
    20521 gtgccggctt tgaagtgcgt gacgtacacc cgactcacta tggtcgttta tgtccaatcg
    20581 aaactcctga aggcccgaat atcggtttga tcaactcact atcggtgtat gctcgtacta
    20641 ataactacgg tttcttagag actccattcc gtaaagtggt gaatggtcaa gtaaccgaag
    20701 agatcgagta tttatctgcg attgaagaaa gcaacaatat cattgcacag gcgaactcaa
    20761 acttagatga gaacttccgt tttaccgata cttatgtaac ttgccgtcag caaggcgaat
    20821 cgggcttatt caaaccggaa gaagttcaat atatggacat ctcaacccaa caagtggttt
    20881 ctgtggctgc ggcgttaatt ccgttccttg agcacgacga tgcgaaccgt gcgttaatgg
    20941 gtgcgaacat gcaacgtcag gcagttccaa ccttgcgtgc ggacaaacca ttagtcggta
    21001 ccggtatgga aaaaccaatt gctcttgact caggtgtagc gattgtcgca aaacgtggtg
    21061 gtacggtgca gtatgttgat gcgtcacgca ttgtagtgaa agtaaatgaa gatgaaacca
    21121 ttccgggcga agcgggtatc gatatttata acttaatcaa atacacccgt tcaaaccaaa
    21181 acacctgtat caaccaagta ccttgtgtga acttgggtga gccggttgct cgtggtgaaa
    21241 tcttagcgga cggtccttca acagacttag gtgaattggc attaggtcaa aatatccgtg
    21301 tggcatttat gccttggaac ggttataact tcgaagactc aatgttagtt tctgagcgtg
    21361 tggtacaaga agatcgcttt accactatcc acattcaaga attatcttgt gtggcgcgtg
    21421 ataccaaatt aggtgcagaa gagatcaccg ctgatattcc aaacgtgggt gaatctgcat
    21481 taagcaaatt agatgaatca ggtatcgttt acatcggtgc agaagtgaaa ggtggcgaca
    21541 tcttagtagg taaagtaact cctaaaggtg aaacccaatt aacaccggaa gaaaaactat
    21601 tgcgtgcgat cttcggtgaa aaagcctctg atgtgaaaga ttcatcattg cgtgttccaa
    21661 acggtacttc aggtacggtt atcgatgttc aagtatttac ccgtgatggc gtagagaaag
    21721 ataaacgtgc gaaagaaatc gaagagatgc aactgtgtga agcgaagaaa gacttagcgg
    21781 aagagttgga aatcttagaa gccggtttat ttactcgtgt tcgcaaccta ttaattgaag
    21841 gcggcgtaaa agaatctgca ttagatggcg ttgctcgtga aaaatggtta gaacaaaccc
    21901 ttgaagacga agcgaagcaa aatcagttag aacagttagc ggaacagcac gaagagttac
    21961 gcaaagaatt tgaacgtaaa cttgagatca aacgcaacaa aattatccaa ggcgacgatt
    22021 tagcaccggg tgtgttaaaa gtagtgaaag tttaccttgc ggtgaaacgc caaatccagc
    22081 cgggtgataa gatggcgggt cgtcacggta acaaaggggt tatctcgaaa atcaaccctg
    22141 tggaagatat gccatacgat gaaaacggtc agccggttga aatcgtattg aacccgctgg
    22201 gcgtaccgtc tcgtatgaac atcggtcaga tcttagaaac ccacttaggc ttagcggctc
    22261 atggtatcgg tgatcagatt gataagatga tcaaacagca acaagaagtg gctaaattac
    22321 gtgaatatat gcaaaaagcc tacgatttag gtcacggctc acaagtcgtt gatttaagca
    22381 ctttctctga tgaagaagtg atgcgtttag cgcaaaactt acgcaaaggc ttaccgcttg
    22441 ctactccggt attcgatggt gcacacgaaa gcgaaatcaa aggcttatta gagttaggcg
    22501 gcttaccgac ttcaggtcag atcacacttt atgatggtcg taccggtgag aaatttgagc
    22561 gtccggtaac cgttggttat atgtatatgc tcaaattgaa ccacttggtt gatgataaaa
    22621 tgcacgctcg ttcaaccggt tcttatagcc ttgttactca acaaccattg ggtggtaaag
    22681 ctcaattcgg tggtcagcgt ttcggtgaga tggaggtttg ggcattagaa gcatacggtg
    22741 cggcttatac tcttcaagaa atgcttacgg tgaaatcgga cgatgtgaac ggtcgtacga
    22801 agatgtataa aaacattgta gatggtacac attatatgga gccgggtatc cctgaatcct
    22861 ttaacgtaat taccaaagag atccgtgcat tagcaattga tatggagctg gacgaagcgt
    22921 aatggtttca ctctccccct tgatggggga gagacaggca aaggatttgt tcagaatcct
    22981 ttggcagaga gagggtgatg caagcggtgt aaatttgcta aatttttgca aaaatcgacc
    23041 gcttaccctc tccctgattt tgcttctgaa cgaggcaaaa tctatccctc tcccacaagg
    23101 ggagagggtt taaaatcgca accaaattcg tagagtggat attctttcca ccaaacaaaa
    23161 caacctcaaa ctaggggcaa aaaagtgaaa gacttagtga agtttttaaa agcacaaaca
    23221 aaatcgaacg aagattttga tgtgattaaa attggtttag catcaccgga caagatccgt
    23281 tcttggtctt ttggtgaagt gaaaaaaccg gaaaccatta actatcgtac cttcaaaccg
    23341 gagcgtgatg gtttattctg tgcacgtatt ttcgggccgg taaaagatta cgagtgcttg
    23401 tgtgggaaat acaaacgttt aaaacaccgt ggcgtaattt gtgaaaaatg tggtgttgaa
    23461 gtaacccaaa ctaaagtacg tcgtgagcgt atggggcaca ttgaattagc ttgccctgtc
    23521 gcacacatct ggttcttaaa atcactaccg tcccgtatcg gtttaatctt agatatgccg
    23581 ttgcgtgata ttgaacgtgt gctttatttt gaatcttatg tagttactga accgggaatg
    23641 accgatttag agaaaaatca gttattaacc gaagaacaat ttatggacgc tgaagagcgt
    23701 tggggtgatg aatttgaagc gaaaatgggt gcagaaggta tccaagcatt acttcgtgat
    23761 ttagatttag aacaccaatg cgaaaccttg cgtgaagagt tacaagaaac caattcagaa
    23821 accaagcgta aaaaaatcac taagcgttta aaaaccttag aagcattctt acaatccggc
    23881 aacaaaccgg aatggatggt gatgacagta ttaccggtac ttccaccgga tttacgtccg
    23941 ttagtaccgc ttgacggtgg acgttttgcg acttctgact taaacgattt ataccgccgt
    24001 gtaattaacc gtaacaaccg tttaaaacgc ttgttagatt tagttgcacc ggacatcatc
    24061 gtgcgtaacg aaaaacgtat gttacaagag tcggttgatg cgttattaga caacggtcgc
    24121 cgtggtcgtg cgattaccgg ttcgaacaaa cgtccgttaa aatctttggc agatatgatc
    24181 aaaggtaaac aaggtcgttt ccgtcaaaac ttattaggta aacgtgtaga ttactcaggt
    24241 cgttcggtaa ttaccgtagg cccatactta cgcttacacc aatgtggttt accgaagaaa
    24301 atggcgttgg aattattccg tccgtttatc tactcaaaat tagaaaaccg tggcattgct
    24361 tcaactatta aagccgcgaa gaaaatggta gagcgtgaag atcctatcgt atgggatatt
    24421 cttgccgaag tgattcgtga acacccgatt ctacttaacc gtgcaccaac acttcaccgt
    24481 ttaggtatcc aagcgtttga gccgttattg attgaaggta aagcgatcca gttacaccca
    24541 ctcgtgtgtg cggcgttcaa tgcggacttc gacggtgacc aaatggcggt tcacgttcca
    24601 ttaacccttg aagcacagtt agaagctcgt gcgttaatga tgtcaaccaa caacgtactt
    24661 tcaccggcaa gtggtgatcc gattatcgta ccgtctcagg acgttgtact cggtctttac
    24721 tatatgaccc gtgagaaagt aaacggtaaa ggtgagggaa tgtacttcct tgaccctcgt
    24781 gaagcggaaa aagcataccg taccggtcag gctgaattac attctcgtgt taaagtgcgt
    24841 gttactgaat atgagaaaaa cgaagcaggc gaattagaag caaaaacttc gttagtagat
    24901 accaccattg gtcgttcaat cttatggatg attgcaccaa aaggtatgcc gtttagcttg
    24961 ttcaaccaaa ccttaggcaa aaaagcgatc tcgaaattaa ttaacgagag ctatcgccgt
    25021 ttaggtttaa aagaatctgt ggttttagct gaccaaatta tgtacaccgg ctttgcttat
    25081 gctgctcgtt ctggtgcctc agtaggtatc gacgatatgg taattccgga gcaaaaatac
    25141 gcaatcattg aagcggctga agcggaagtc gctgagatcc aagagcaatt caacggcggt
    25201 ttagtaacag ccggtgagcg ttataacaaa gtgatcgata tttgggctgc tgcaaacgaa
    25261 cgtgttgcga aagcaatgat ggaaaacctt tcaacggaag aagtaatcaa ccgtgaaggt
    25321 aatccggaaa aacaagcctc atttaacagt atctttatga tggcggactc cggtgctcgt
    25381 ggttctgctg cacagattcg tcagttagcg ggtatgcgtg gcttgatggc tcgtccggac
    25441 ggttcgatta tcgaaacccc aattactgca aacttccgtg aaggtttgaa cgtattacag
    25501 tactttattt caacccacgg tgcacgtaaa ggtcttgccg atacggcatt aaaaacagcg
    25561 aactccggtt acttaacccg tcgtttagtt gatgtggcac aagatttagt tatcattgaa
    25621 gatgactgtg gtacgcacga aggtatcgtg atgacaccac ttatcgaagg tggtgatgtg
    25681 aaagaagccc tgcgtgatcg tgtattaggt cgtgtggtgg cagaagatgt gttaaaaccg
    25741 ggtacagatg aagtattaat tccacgcaac accttattag atgaaaaatg gtgtgatgtg
    25801 gtggatcgtg aatctgtgga tgtgatcaaa gtacgttcgg tagtaacttg tgatacagac
    25861 ttcggtgtgt gtgctaaatg ttacggacgt gacttggctc gtggtcactt aatcaaccaa
    25921 ggtgaagcgg tcggtgttat tgcggcacaa tctatcggtg agccgggtac acagttaacg
    25981 atgcgtacgt tccatatcgg tggtgcggct tctgcggcag caaaagaatc tagcatccaa
    26041 gtgaaaaacg caggtacgat taaattaacc aacgctaaat ttgtaaccaa caaagaaggc
    26101 aaaatcgtat taacttctcg taatactgaa ttaactgtca tcgacacctt cggtcgtacc
    26161 aaagaaaact ataaagtgcc ttatggtgcg gtgctttcta aacacgacgg tgcagaagtg
    26221 gctgtgggtg aagtggttgc gaactgggat ccgcacacaa tgccggtaat ctcagaggta
    26281 tcaggtcgta tccaatttag cgacatcgta gatggcttaa ccgttactcg ccaaaccgat
    26341 gaattaaccg gtttatcatc tatcgtggtg caagatgtgg gtgaacgtgc aaccgcaggt
    26401 aaagatttac gtccggcatt acgcttagtt gatagcaacg gcaaagacat cttaattccg
    26461 ggtacagacg ttgcagcaca atacttctta ccgggtaaag caatcgtaac cttagacgat
    26521 ggtgcagaaa ttgaagtcgg tgaagcctta gcccgtattc cacaagaatc tgtggggacg
    26581 aaagatatta ccggtggtct tccacgcgta gcagacttat tcgaagcccg taaaccgaaa
    26641 gagccggcaa tcttggcgga aatttcaggt attgtgtcgt tcggtaaaga aaccaaaggt
    26701 aaacgccgct tggtaattac tccggcggaa ggcgaagcgt ttgaagagat gattccaaaa
    26761 tggcgtcagc tcaacgtatt tgaaggcgag atggtacaac gtggtgatgt gatatcagac
    26821 ggtgcagaaa ctccgcacga catcttacgc ttacgtggcg ttcacgccgt taccgactac
    26881 atcgtaaacg aagtgcaaga agtttaccgc ttacaagggg taaaaattaa cgacaagcac
    26941 atcgaagtga ttgttcgcca aatgttgcgt aaagctgtga tcaccaacgc ttacgactct
    27001 gaattcttag agggcgaaca agtggaagtg gctcgtgtga aaattgccaa ccgcaaacgt
    27061 gctgaagaag gcaaaccgtt ggtagaattt gaacgtgaat tgcttggtat taccaaagca
    27121 tctcttgcaa cagaaagctt tatttctgca gcctcgttcc aagaaaccac tcgagtgctt
    27181 accgaagccg cagtggcagg taaacaagat gaattgcgtg gcttaaaaga gaacgtaatc
    27241 gttggtcgct taatcccggc aggtaccggt tttgcttacc accaagcacg agctaaaaaa
    27301 cgtgcccaac tggaagcagg aattgcagtt gtccctgaat ttgaagcacc ggcagctaaa
    27361 gccaattcat tcgttaccga tgcagacatc gaagctgaat ttgaatttgt tgcagacgat
    27421 gcaacccaaa gtttagcagc gttgttaaat gcgggtgagg aagagtaata gctcgtaggg
    27481 tatgataaat taaaaaatct cctagaatat aatttctagg aggttttcta ct
//
LOCUS       DANLXF010000004        25884 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.004, whole genome shotgun sequence.
ACCESSION   DANLXF010000004 DANLXF010000000
VERSION     DANLXF010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 25884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25884
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.004"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..910
                     /locus_tag="PXD66_000103"
     CDS             <1..910
                     /locus_tag="PXD66_000103"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015701738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HDL6318445.1"
                     /translation="IIEVQGPILSEVGNGMQDNLSGIEKAVKVNVKCIPSATYEVVHS
                     LAKWKRHTLARFGFKEGEGLFVHMKALRPDEDSLDRTHSVYVDQWDWEKVIPEGRRNF
                     DFLKETVNEIYKAIRLTELAVEARFDIPSVLPKQITFVHSEDLVQRYPNMTSKERENA
                     ICKECGAVFLIGIGGKLSDGKPHDGRAPDYDDWTTESEQGYKGLNGDILVWNEELGTA
                     FELSSMGIRVDETALRLQVALTGDEDRLEMDWHKDLLAGRLPLSIGGGIGQSRLVMFL
                     LRKKHIGEVQSSVWPKAVFEEFENIL"
     gene            complement(1034..1453)
                     /gene="atpC"
                     /locus_tag="PXD66_000104"
     CDS             complement(1034..1453)
                     /gene="atpC"
                     /locus_tag="PXD66_000104"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDL6318446.1"
                     /translation="MASQFELTVVSAERKIFEGTVTSVRVSGTDGELGVYAGHAPLLT
                     AIKPGMVKFTLADGKEEFIYVSGGFLEVQPTVVTVLADTAIRGEELDEQRILAAKRKV
                     EETLSKTSDADMTAKLAREIAKLRVYELTKTKLANKR"
     gene            complement(1481..2854)
                     /gene="atpD"
                     /locus_tag="PXD66_000105"
     CDS             complement(1481..2854)
                     /gene="atpD"
                     /locus_tag="PXD66_000105"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011978979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDL6318447.1"
                     /translation="MATGKIVQIIGAVIDVEFPQDAVPKVYDALKVESGLTLEVQQQL
                     GGGVVRCIALGSSDGLKRGLKVENTNKAIEVPVGTKTLGRIMNVLGEPIDEAGPIGEE
                     ERWTIHRAAPSYEEQANSTELLETGIKVIDLVAPFAKGGKVGLFGGAGVGKTVNMMEL
                     IRNIAIEHSGYSVFAGVGERTREGNDFYHEMKDSNVLDKVSLVYGQMNEPPGNRLRVA
                     LTGLTMAEKFRDEGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEMGVL
                     QERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDSTVVLSRNIASLGIYPAVDP
                     LDSTSRQLDPLVVGEEHYNVARGVQGTLQRYKELKDIIAILGMDELSEDDKRLVARAR
                     KIERFLSQPFHVAEVFNGVPGKFVPLKETIRGFKGILEGEYDHIPEQAFYMAGSIDEV
                     VERAKQM"
     gene            complement(2875..3741)
                     /gene="atpG"
                     /locus_tag="PXD66_000106"
     CDS             complement(2875..3741)
                     /gene="atpG"
                     /locus_tag="PXD66_000106"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011201492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDL6318448.1"
                     /translation="MAGAKEIRTKIASVRNTQKITKAMEMVAASKMRKTQERMAASRP
                     YAESIRKAISHIAKGNIEYKHPFLTPRPVKKVGYLVVSTDRGLCGGLNINLFKTVLHE
                     LKEKDDQGVKSRLAVVGNKGISFFNPMGLEIKGHINGLGDTPAMEDLVGIVNGMVNAY
                     REGEIDEVYVVYNRFINTMSQKPTVQQLLPLPALENDSLEQTGSWDYLYEPNPQALLD
                     SLLVRYLESQVYQAVVDNLASEQAARMVAMKAATDNAGNLINELQLVYNKARQASITN
                     ELNEIVAGAAAI"
     gene            complement(3767..5308)
                     /gene="atpA"
                     /locus_tag="PXD66_000107"
     CDS             complement(3767..5308)
                     /gene="atpA"
                     /locus_tag="PXD66_000107"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDL6318449.1"
                     /translation="MQLNSTEISELIKKRIAQFNVVSEAQSTGTIVSVSDGVIRIHGL
                     SDVMQGEMIALPGNRYAIALNLERDSVGAVVMGPYADLAEGMQVQCTGRILEVPVGRG
                     LLGRVVNTLGQPIDGKGEIQNDGFSPIEVIAPGVIDRQSVDQPVQTGYKAVDSMVPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTIANVVRKLEEHGAL
                     ANTIVVVASASESAALQYLAPYAGCAMGEYFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERASRVNADYVERFTNGAVKGQTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLESGLFNAGIRPAVNPGISVSRVGGSAQTKLVKKLAGG
                     IRTALAQYRELAAFAQFASDLDEATRKQLSHGQKVTELLKQKQFEPMSVAQLGLSLAA
                     AEYGYLDDIPVERVGSFEANLLAYAQSNYGEFMQELSKSGNFNDEIKDKLNEILSGFK
                     KNSAW"
     gene            complement(5321..5854)
                     /gene="atpH"
                     /locus_tag="PXD66_000108"
     CDS             complement(5321..5854)
                     /gene="atpH"
                     /locus_tag="PXD66_000108"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDL6318450.1"
                     /translation="MSELSTVARPYAKAAFDFALEQGQLEQWQQMLTFSAAVANDEQV
                     ASFLNSSLASGQISETFIKICGEQLDQYGQNFIRVMADNKRLAVLPAVLNAFLELRAE
                     QESIKDVEVVSADKLTDAQEAKIANAMEKRLGTKVRITSSIDSSLIAGVIIRYDDVVI
                     DGSSRGQLNRLSQELSL"
     gene            complement(5868..6338)
                     /gene="atpF"
                     /locus_tag="PXD66_000109"
     CDS             complement(5868..6338)
                     /gene="atpF"
                     /locus_tag="PXD66_000109"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDL6318451.1"
                     /translation="MNLNATLIGQLIAFALFVAFCMKFVWPPLIRVIEERQANIASAL
                     ASAEKAKQEQADSKAAADQEILRAKEEAQNIIDLATKRRNEILESVQAEAEVERQRII
                     EQGYAEVESERKRVQEELRQKVAALAIAGAEKIVGRSVDAAANNDIIDKLVAEL"
     gene            complement(6399..6653)
                     /gene="atpE"
                     /locus_tag="PXD66_000110"
     CDS             complement(6399..6653)
                     /gene="atpE"
                     /locus_tag="PXD66_000110"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HDL6318452.1"
                     /translation="MESVITATIIGASILLAFAALGTAIGFAILGGKFLESSARQPEL
                     ASSLQTKMFIVAGLLDAIAMIAVGISLLFIFANPFIDLLK"
     gene            complement(6771..7559)
                     /gene="atpB"
                     /locus_tag="PXD66_000111"
     CDS             complement(6771..7559)
                     /gene="atpB"
                     /locus_tag="PXD66_000111"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDL6318453.1"
                     /translation="MAGTTAEYISHHLSFLSTGEGFWAVHLDTLFFSLVAGAIFLWVF
                     SRVAKNATTGVPGKLQCFVEIVIEWVNGLVKDNFHGPRNVIAPLALTIFCWVFIMNAI
                     DLIPVDFLPQLANLFGIHYLRAVPTADISATLGMSICVFALILFYTVKSKGFGGLVKE
                     YTLHPFNHWAFIPVNFILETVTLLAKPISLAFRLFGNMYAGELIFILIAVMYMADNFA
                     LQALGIPLHLVWAIFHILVITLQAFIFMMLTIVYLSIAYNKADH"
     gene            complement(7582..7965)
                     /locus_tag="PXD66_000112"
     CDS             complement(7582..7965)
                     /locus_tag="PXD66_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HDL6318454.1"
                     /translation="MSAVINRAKSQYKQALKVEFFLLCVFALGVSLWKMNFNFSFLGG
                     ALASFLPHLVFVYWVFFKKSAKNQSKMTAFYRGEGMKWLVTIILVIGCFKLIPALDVV
                     LFFVGYFIALFLNNMIPFVLSKHHH"
     gene            8433..9566
                     /gene="carA"
                     /locus_tag="PXD66_000113"
     CDS             8433..9566
                     /gene="carA"
                     /locus_tag="PXD66_000113"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDL6318455.1"
                     /translation="MFKPAILVLTDGSVFSGKSIGADGHTIGEVVFNTAMTGYQEILT
                     DPSYTKQIVTLTYPHIGNTGINLEDKESPNVCASGLIIRDLPLLHSNFRASSSLSDYL
                     IQQNIVAIADIDTRRLTRILREKGAQAGCILVGDATEENIANALAFANSFGSMAGKDL
                     AQSVTCNELYKWTDGEWQLGKGYVPQQQTEFHVVAYDYGVKHNILRMLAERGCRLTVV
                     PAKTSAEAVLALNPDGIFLSNGPGDPEPCDYAITAIRKLLETQKPIFGICLGHQLLGL
                     AAGGKTKKMVFGHHGANHPVQDLDSQKVLITSQNHGFEVDESSLPSNVRVTHRSLFDG
                     TVQGIELTDRPAFSFQGHPEASPGPNDVAYLFDRFVSEMKKVK"
     gene            9669..12875
                     /gene="carB"
                     /locus_tag="PXD66_000114"
     CDS             9669..12875
                     /gene="carB"
                     /locus_tag="PXD66_000114"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDL6318456.1"
                     /translation="MPKRTDINTILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     VLVNSNPATIMTDPNMADVTYIEPIEWKTVEKIIEKERPDAILPTMGGQTALNCALDL
                     SKNGVLKKYNVELIGATEDAIDKAEDRGRFKEAMTKIGLNTPKSFVCHSFEEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNRDEFIAICERGFEASPTHELLIEQSVLGWKEYEM
                     EVVRDRADNCIIVCSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAINPANGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLNE
                     LRNDITGGLIPASFEPTLDYVVTKIPRFAFEKFANADDRLTTQMKSVGEVMAMGRTFQ
                     ESLQKALRGLETGICGFNLLSEEPEKIRRELANPGPNRILYVADAFGAGFSLEEVHHY
                     SKIDPWFLVQIQDLVLEELALEKKQFSDLDANEIRRLKRKGFSDKRIAQLTKVSEKMV
                     RDYRHTYAILPVYKRVDTCAAEFASDTAYLYSTYEEECEAKPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHASLALREAGYETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RVENPWGVIVHYGGQTPLKLANALSDAGVNIIGTSADSIDAAEDRERFQQILNTLGLK
                     QPANRTARNALEAVALANEVGYPLVVRPSYVLGGRAMQIVYNDEELNRYMREAVSVSN
                     DSPILLDHFLNNAIEVDVDCICDGENVLIGGIMQHVEQAGIHSGDSACSLPAYSLSGH
                     VLDEIRKQTNAMAFALGVKGLMNVQFAVQNEVVYVLEVNPRASRTVPFVSKATGRPLA
                     KIAARVMAGETLADLNALEEIIPSKFFVKEAVFPFIKFPGVDTILGPEMRSTGEVMGI
                     GNTFAEAFFKAQLGAGERIPKVGKVFMSVIEEHKPALIYIAKRFQEQGYGLCATFGTA
                     NFLRQHGIAVQTVNKVREGRPHIVDAIKNGEIALVINTVSGEPEVIADSHSIRRTALQ
                     QRVPIYTTLAGADAISYGVKHINEFDVYSVQELEQKAN"
     gene            complement(12950..13741)
                     /locus_tag="PXD66_000115"
                     /pseudo
     CDS             complement(12950..13741)
                     /locus_tag="PXD66_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012072249.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
     gene            13944..14942
                     /gene="yiaK"
                     /locus_tag="PXD66_000116"
     CDS             13944..14942
                     /gene="yiaK"
                     /locus_tag="PXD66_000116"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005757429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HDL6318457.1"
                     /translation="MRVSYEELKAQFKRVLLARNVREEIAEECATVFADTTQAGAYSH
                     GVNRFPRFIQQLENGDIVPEAVPTKVLSLGAIEQWDAHQAIGNLTAKKMMDRAMELAS
                     ENGIGVVALRNANHWMRGGSYGWQAAEKGYIGICWTNALAVMPPWGAKECRIGTNPLI
                     IAVPTNPITMVDMSCSMYSYGMLEVHRLAGRQTFVDAGFDDDGNPTRDPATVEKNLRL
                     MPMGFWKGSGLSIVLDMIATLLSNGESTAAVTEDKDDEYCVSQVYIAIEVDRLIDGKT
                     KDEKLNRIMEYVKTAERADPDVAVRLPGHEFTQILADNKANGIPVDDTVWAKLQAL"
     gene            15067..15432
                     /locus_tag="PXD66_000117"
     CDS             15067..15432
                     /locus_tag="PXD66_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDL6318458.1"
                     /translation="MFFGHISQVKANRYPPAIQIALNYLKNTDFDAMEAGVYELKGRQ
                     IYVQVLDLNTKSKHEFQPEVHRNYLDVQYLHRGKEIMAAAVDTGTNPIAMEYNPERDI
                     QYYQSVANENEFRCVEGNF"
     gene            15496..15975
                     /locus_tag="PXD66_000118"
     CDS             15496..15975
                     /locus_tag="PXD66_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDL6318459.1"
                     /translation="MKALAQLVGKLLEILVVVILSAMSILVFVNVVLRYGFNSSISVT
                     EEVSRYMFVWLAFLGAVLAFSENQHVKVTMLTDKLSPTARNILSVVTDLAMLFCCYLI
                     VDGSWIQFNLNLNNFAPISGLPQGITFLASTVAGVLIGLLIIARIVATVGVIAKGEK"
     gene            15976..17253
                     /locus_tag="PXD66_000119"
     CDS             15976..17253
                     /locus_tag="PXD66_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017806296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HDL6318460.1"
                     /translation="MTVVIFLSVLLGSILLGIPVAFALLVCGVALMLHLDLFDAQILA
                     QQIVSGADSFSLMAIPFFILAGEIMNEGGLSKRIIDLPMKLVGHKRGGLGFVAILAAM
                     IMASLSGSAVADTAAVVAMLLPMMKTTGYPIDRSAGLIGTAGIIAPIIPPSIPFIVFG
                     VASGVSITKLFLAGIFPGIMMGICLAALWWWQAKRLDLMTFSKATKEELCISFKNSIW
                     ALLLPVIIIGGFRSGMFTPTEAGAVATFYALIVSLFVYREMKFKDLYKVVLAAAKTTA
                     VVMFLVAAANVTGWLITVAELPMMLTELLEPLIESPTTLLLVIMLAVFIIGMVMDLTP
                     TVLILTPVLMPLVEEAGIDPVYFGVLFILNTSIGLITPPVGNVLNVITGVSKLPFDQA
                     AKGVMPYLVMMICLLLLFVFFPSLVLVPLSWMQ"
     gene            17356..18342
                     /locus_tag="PXD66_000120"
     CDS             17356..18342
                     /locus_tag="PXD66_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDL6318461.1"
                     /translation="MKLFNLKTLFALVAGVAVMSGSAMAETSLRFGYEAPRSDTQHIA
                     AKKFDELLAAKTKGEIKLKLFPDSTLGNAQTMISGVRGGTIDIEMSGSPNFTGLEPKL
                     NVIDIPFIFKDREHVYKVLDGEVGQGLLKDLEAQGLKGLAFWDVGFRAFSNSKHPVTK
                     PEDIKGLKVRTNQNPMYIQAFTLLGGNPVPMPLSELYTALETRAVDAQEHPIGIFWSA
                     KLFEVQKHLSLTNHGYTPLIVVMNKAKFDGLAPELQTALVEAAKEAGQFQRDLNVKNE
                     KEIIEKLRKQGIEVIEQVDNAPFKAAIEAEVRKAFVEKHGDELLKKVDALAQ"
     gene            18416..19399
                     /locus_tag="PXD66_000121"
     CDS             18416..19399
                     /locus_tag="PXD66_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDL6318462.1"
                     /translation="MKLFNLKTLAAFVAGFALFSTAQAETSFRFAYEAPRSDTQHIAA
                     KKFNDLLQEKSQKGLKLSLFPDSTLGNAQTAISGVRGGTIDIAMSSSSNFTGLEPKLN
                     VIDIPFIFKDRDHAYKALDGEIGQGLLTALEAHGLKGLAFWEVGFRAFSNSKHPITKP
                     EDIKGLKIRTNQNPMYIQAFSILGANPVPMPLSKLYTALETKAVDAQEHPIGIFWSAK
                     LYEVQKYFSFTNHGYTPLIVAINKAKFDALPAEQQTALVEAAKEAGKYQRQLNLENEQ
                     KIIDSLKKAGIEFVDNLDTTPFKDAVSAETRKAFIEKNGDGLVKAIDALAK"
     gene            19530..21014
                     /locus_tag="PXD66_000122"
     CDS             19530..21014
                     /locus_tag="PXD66_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL6318463.1"
                     /translation="MQHIIKRFGSHTAVNDVSLDIHSGEIVALLGENGAGKSTLIKIL
                     AGIYDRDEGDILFHNQKIQSAHAIEKGNKKPIAFIHQDLGLIEWMTIAENMAFVMGFP
                     RKFGLIDWAKVREQSQKALDFVGIKLSADTRVFDLSRTEKALLAIARAIAVNAEILVL
                     DEPTASLPASDVEHLFTVLNRLRSQGVGMIYVTHRLDEVIAISDRILVMRDGFPVAEG
                     QTKLYDVKGLVKAIVGEESRGRQRVDLPTDTPEILSLQNVVVGDTGPVNFRLHKGEMI
                     ALAGLRGAGQEEIGRLLFGERTLDKGEIQLDKRSYSAKDPQDAIAKGVALVAGDRTRE
                     SVFMSMTTSENLFINPVLSGHSPLKRYSRREEWGESWYKFQLFDIRPKNLFIDASALS
                     GGNQQKIVLARWMHLNTPVLILEDPTAGVDVGARAEIYDLLNKALAEGVAIIVISTDF
                     EEIAHLCNRALVFNRGEIAGELLNEEVTFANLLALASDSQSKKQ"
     gene            21209..22255
                     /locus_tag="PXD66_000123"
     CDS             21209..22255
                     /locus_tag="PXD66_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL6318464.1"
                     /translation="MAQTNIKSTALEQPVSIARDGFMAWFSQMSTRYGLLWLCVLLVI
                     IFSLTTESFASMLTLNAILESKSKIALLALAATTTMIVGKIDLNVGFGIVLWHILVIT
                     LQVQYGFSWYMAILIVLVIAALYGLLNGILVALADIDSFVATLGSGTVLYAVALWHSG
                     GRQIVGDLPDEFIALNSTEIFGIPISAFYVLAVAIVMWLVTEHTPTGRCMYAVGGNPT
                     AARLNGINIKKYTIVPFIVSSVITAFTGVLISAQQGVGQASVGMDYLLPALVGAFLGS
                     TTIRPGRINVWGTVVGIAILAIGISGIQQFGGAFWVEPLFNGATLLLSITIAGYAQRK
                     RLLNQKAVQKKSTN"
     gene            22268..23377
                     /locus_tag="PXD66_000124"
     CDS             22268..23377
                     /locus_tag="PXD66_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005757420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL6318465.1"
                     /translation="MKKAFRTTLTAIATLTAIAFGNNAFANDDPFVQEAKQLVAAATA
                     KQEKWDGPTTGPQLQKEKTVIFIASDMKNGGVLGVIDGMKEAVGVTGWKLDVLDGAGT
                     VNNQLAALNQAIARKPDAIVIGGWNPNVAKIPLQKATKNGIKLAAWHATPEPGAIDKY
                     GIFYNVTSDSDEIARLSAMLAVAESNGKAKAIILTDSLYEIALRKANVMKEVIEKCKE
                     CKVLEFIDTPLADTSSRMPNLTFSLLQKYGDDLEYALAINDLYFDFMAPSLRTAGKDK
                     PFSISAGDGSVTAYQRIRNGDKQFATVPEPLNLHGWQLVDELNRAFANEKPSGYITPA
                     HLVTKENVAFDGGENNLYAPQNGYKGAYKAIWGVK"
     gene            23482..24372
                     /locus_tag="PXD66_000125"
     CDS             23482..24372
                     /locus_tag="PXD66_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HDL6318466.1"
                     /translation="MSNKFIPKISNPRFNELVGNTFELEELFDKAIWAEGPAWSKPQQ
                     ALYFSDVKGCTMYRWTEQDGTQVFRQPSYFSNGNAFDANDNLITCEHGNRGISLTTPS
                     GEYRFLVGQLDGKRLNSPNDVVVKSDGIIWFTDPPYGILSDNEGKQSPSEIIGCYIYC
                     YDPKTDKLNIATFNTMRPNGLAFSSDEKQLFVADMSSVEFDEDGLHHLVVFDVDGNKL
                     KNRRDVAEISPGIPDGFRIKQNGVIFCSCEEGIIALLPDGTELGRFVLGKLTSNCSFG
                     DDEQTLFITCSNSVYRLTIK"
     gene            24444..>25884
                     /locus_tag="PXD66_000126"
     CDS             24444..>25884
                     /locus_tag="PXD66_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HDL6318467.1"
                     /translation="MDNKMNYTLGIDCGGTFIKAALFDTQGNIQALHRENVSVISEQA
                     GYAERDMSQLWAICAEVVRSTIEKSGVNPADIKAVGISAQGKGAFLLDKQNRPLGRAI
                     LSSDQRSLEIVKQWQAEGIPQQLYPITRQTLWTGHPVSILKWVQQNEPERYAQIGAVL
                     MSHDYLRFCLTGELHCEETNISESNLYNMEKGEYDQQLADLLGMPDVLEKLPPVIEPN
                     KISGYVTENAAKLTGLAVGTPIVGGLFDVVSTARCANLDDETKLNAVLGTWSVVSGIT
                     DHIDASQSLPFVYGRYAEAGKFIVHEASPTSAGNLEWFVNQWKLDYVKINQAVAQLPP
                     AASSVLFVPFLYGSNAGLGMQAGFYGMQAHHTQAHLLQAIYEGVLFSLMSHLNRMFIR
                     FPKADVLRVTGGPVKSKVWMQMLADLTGMRLEIPQMEETGCLGAALMAMQGVGLDISQ
                     VQALNNEMQVFEPNPENYAAYQTKYQRYQK"
BASE COUNT         7402 a         5717 c         5684 g         7081 t
ORIGIN      
        1 cattattgaa gttcaggggc ctattttgag cgaagtcggc aacggtatgc aagacaacct
       61 ttccggtatc gaaaaagcag taaaagtaaa cgtaaaatgt attccaagcg ctacttatga
      121 agtggttcat tctcttgcaa aatggaaacg ccacacctta gctcgttttg gatttaaaga
      181 aggagaaggg ttatttgttc atatgaaagc tcttcgccct gatgaagatt ctttagatcg
      241 cacacactcg gtatatgttg accaatggga ctgggaaaaa gtcattcctg aaggtcgccg
      301 caacttcgat ttcttaaaag aaacggtaaa cgaaatctat aaagcaattc gtttaaccga
      361 attagcggtg gaggctcgtt tcgatattcc atctgtattg cctaaacaaa ttactttcgt
      421 tcacagcgaa gacttagtgc aacgctatcc gaatatgacc agcaaagagc gtgaaaatgc
      481 tatctgtaaa gagtgcggag cggtgttctt aatcggtatc ggcggcaaat tatcagacgg
      541 taaacctcac gatggacgag ctccggacta tgatgactgg acaaccgagt ccgaacaagg
      601 ctacaaaggc ttaaacggcg atattttagt ttggaacgaa gaactcggca cagcctttga
      661 actttcctca atgggtattc gtgtagatga aaccgcctta cgcttacaag ttgctttaac
      721 tggcgacgaa gaccgcctcg aaatggattg gcacaaagat ttattagccg gcagactccc
      781 gctttctatc ggcggcggta tcggtcaatc tcgcttagtg atgttcttac tacgcaaaaa
      841 acacatcggt gaggtgcaat ccagtgtctg gccaaaagcg gtatttgaag agtttgagaa
      901 tattctttaa taaaacaaaa aagcgaacct tagggttcgc ttttgctttc tacaagcggt
      961 cttattttgc aaaaaattta ctgtttttaa ccgcttgtaa aacaaaaccc cactttcgtg
     1021 gggttttttg atattaacgt ttattcgcta atttggtttt agtgagttcg taaacacgca
     1081 gtttcgcaat ttctctcgct aacttcgcgg tcatatccgc atcgctggtt tttgaaagtg
     1141 tttcttctac tttgcgtttt gcggcaagaa tacgctgttc gtctagttct tcaccacgaa
     1201 ttgcggtatc agccaacact gttactacgg tcggttgtac ttcaaggaaa ccacctgaaa
     1261 cgtagataaa ctcttctttg ccgtcagcta aagtgaattt aaccattccc ggtttaattg
     1321 cggttaataa cggtgcatgc cctgcataca cccctaattc accatctgtc cctgaaacac
     1381 gcacgctggt aactgtgcct tcaaagattt tacgttctgc actcactacc gtgagttcaa
     1441 attgagatgc catgttgttc tcctttagtt tagaacaaaa ttacatctgt tttgctcttt
     1501 caaccacttc gtcaattgaa cccgccatat agaacgcttg ttccggaata tggtcgtatt
     1561 cgccttctaa aatgccttta aagccacgaa ttgtttcttt taacggtacg aatttgcccg
     1621 gtacgccgtt gaatacttcc gcaacgtgga atggttgtga aaggaaacgt tcgattttac
     1681 gcgcacgagc aacaaggcgt ttgtcgtctt ccgataattc atccataccc aaaattgcaa
     1741 tgatgtcttt taactcttta taacgttgta atgtaccttg tacaccacga gcaacgttat
     1801 agtgttcctc accaaccacg agtggatcta actgacgaga ggttgagtca agagggtcaa
     1861 ccgccgggta aataccgagt gaagcgatat tacggcttaa taccaccgtt gagtctaagt
     1921 gggcaaaggt agttgccgga gacgggtcag ttaagtcatc cgccggaaca tataccgctt
     1981 gcacagaggt aatagagccg gttttggttg aggtaatacg ctcctgtaat acgcccatct
     2041 cttctgcaag agtcggctga taacctaccg cagacggcat acgacctaat aacgcagata
     2101 cttcggtacc agctaaagta taacggtaga tgttatccac gaagaataat acgtcacgac
     2161 cttcatcacg gaatttttct gccatggtta agccggttaa tgccacacgc agacggttgc
     2221 ccggtggttc gttcatttga ccgtaaacca gcgatacttt gtctaatacg ttagagtctt
     2281 tcatttcgtg gtagaagtca ttaccctcac gagtacgctc acctacaccg gcgaataccg
     2341 agtaaccgct atgttcgata gcgatgttac ggattaactc catcatattt acggttttac
     2401 ctacacccgc accaccgaat aagccaactt taccaccctt agcaaatgga gcaactaagt
     2461 cgataacttt gataccggtc tctaaaagct cggtgctgtt cgcttgttct tcgtagcttg
     2521 gtgctgcacg gtggattgtc caacgttctt cttcaccgat tgggcctgct tcatcaattg
     2581 gctcacctaa tacgttcata atacgaccga gtgtttttgt accaaccggt acttcaatcg
     2641 ctttattcgt attttcaact tttaagccac gttttaaacc atcagatgaa ccaagtgcga
     2701 tacaacggac cacaccgcca cctaattgtt gttgaacttc aagggttaat cctgattcaa
     2761 cttttaatgc atcataaact tttggtactg catcttgcgg aaactcaaca tcgattaccg
     2821 caccgatgat ctgtacaatt tttcccgttg ccattaccgt tcctctattc attgttaaat
     2881 tgctgcggca cccgcgacaa tttcatttaa ttcattcgta atacttgctt gacgagcttt
     2941 gttatacact aactgtaact cattaatcag attacctgcg ttatcggttg ctgctttcat
     3001 tgccaccatt cgagcagcct gttcagacgc aagattatct accactgcct gataaacttg
     3061 agattctaaa taacgaacca gtaagctgtc taataacgct tgtggatttg gttcatagag
     3121 ataatcccaa gaaccagttt gctctaatga gtcattttcc agtgcaggca aaggaagcaa
     3181 ctgttgtact gtcggttttt gtgacatcgt gtttataaaa cggttatata ccacatacac
     3241 ttcatcaatt tcgccttcac ggtaggcatt taccatacca ttaacaatac cgactaaatc
     3301 ttccattgcc ggtgtatcac ccaatccatt gatatgacct ttaatctcta gccccattgg
     3361 gttaaaaaag gagatccctt tatttcccac cacagcaagt cgagacttaa caccttggtc
     3421 atctttttct ttcaattcat gtaaaacggt tttaaataaa ttgatattta agccaccaca
     3481 taaaccgcga tcggttgaaa ctactaaata gccaactttt tttaccggac gtggggtcaa
     3541 aaatgggtgt ttatactcaa tgttaccttt ggcaatatgg ctaattgcct tgcgaatact
     3601 ttcagcataa gggcgagaag ccgccatacg ctcttgggtt ttacgcattt ttgatgcggc
     3661 aaccatttcc atcgctttgg taattttttg tgtattacga acacttgcaa ttttggttct
     3721 tatctcttta gcacctgcca tattatttct ccgctaaaaa gttagattac caagcactgt
     3781 tctttttgaa accagataaa atctcgttca atttatcttt aatttcatcg ttgaaattgc
     3841 cggattttga cagttcttgc ataaactcgc cgtagttact ttgagcataa gctaataagt
     3901 ttgcttcaaa tgagccaaca cgctcaaccg gaatatcatc taagtaaccg tattcagctg
     3961 ctgctaatga taaacctaat tgtgcaacag acatcggctc gaattgtttt tgtttcaaca
     4021 attcagttac tttttgaccg tgtgaaagct gtttacgtgt tgcttcatca agatctgatg
     4081 cgaactgggc aaatgccgct aattcacggt attgagcaag tgcggtacgg ataccaccgg
     4141 caagcttctt aaccagttta gtttgtgcag aaccacctac acgggaaacc gagatacccg
     4201 ggtttaccgc agggcgaata ccggcattga ataagcctga ttctaagaaa atctgaccat
     4261 cggtaattga aattacgtta gtcggtacga aggctgaaac gtcacctgct tgggtttcga
     4321 tgatcggtaa tgcggttaaa gaaccggttt gacctttcac agcaccgtta gtgaaacgtt
     4381 ctacatagtc tgcatttaca cgagacgcac gctctaataa gcgagagtgt agatagaaca
     4441 catcacccgg gaatgcttca cgacccggtg gacggcgtaa taacaatgaa atttgacggt
     4501 aagcaactgc ttgcttagac aaatcatcgt aaacgatcaa cgcatcttca ccacggtcac
     4561 ggaagtattc acccattgca caacctgcat aaggtgcaag atattgtaat gcagcagatt
     4621 cagacgcaga agccactaca acgatagtgt tcgctaatgc accgtgttct tctaatttac
     4681 gcaccacgtt ggcgatagtt gatgcctttt gaccgattgc aacgtagata catttaatgc
     4741 ctgaatcacg ttggttgatg atcgcatcaa tcgctaatgc ggttttaccg gtttgacggt
     4801 cacctataat caattcacgc tgaccacgac cgattggtac cattgagtcc accgctttgt
     4861 aaccggtttg aaccggttga tcaaccgatt gacggtcgat aacgcccggt gcgatcactt
     4921 cgattggtga gaaaccgtca ttttggattt cgcctttacc atcgattggc tgacctaagg
     4981 tatttacaac acgacctaat aagccacgac caaccggcac ttctaaaata cgaccggtac
     5041 attgtacttg cataccttct gctaaatcgg cataaggtcc cattacaact gcaccaacag
     5101 aatctctttc taagtttaat gcaatcgcat aacgattgcc cggtaatgca atcatttcac
     5161 cttgcattac atcagataag ccgtggatac gaataacccc gtcacttacc gaaacgattg
     5221 tccctgtgct ttgagcttca ctcaccacat taaactgggc aatacgtttt ttaatcaatt
     5281 cactaatttc agttgaattt agttgcattt tttattcctc ttacaagctc aactcttgac
     5341 tcaagcggtt tagctgtcca cgactactac catcaatcac tacatcatca taacgaatga
     5401 tcacacccgc aattaatgaa ctatctattg atgaagtaat acgtactttc gttccgagtc
     5461 gcttttccat cgcattggca atttttgctt cttgtgcatc tgttaattta tctgcagaaa
     5521 caacctcaac atctttaatt gattcctgtt ctgctcttaa ttctaaaaat gcattcaata
     5581 ccgcaggtaa tactgctaaa cgcttgttat cagccattac gcgaatgaaa ttttgcccat
     5641 attgatctaa ttgttcgcca caaatcttga taaacgtctc agaaatctga ccgcttgcaa
     5701 gtgatgagtt aagaaaacta gctacttgct catcgttagc gacggcggca gaaaaagtta
     5761 acatctgctg ccattgttcc aattgacctt gttctaaagc aaaatcgaaa gccgctttag
     5821 cataggggcg agctactgtg cttaattctg acatctgccc ctcctagtta tagttccgca
     5881 actagcttat caataatatc gttgttcgca gcggcatcca ccgaacgacc cacgattttt
     5941 tctgcaccag caatagccaa tgcagccact ttttgacgaa gctcttcttg aacgcgttta
     6001 cgttcgcttt caacttccgc gtaaccctgt tcaataatac gttgacgttc cacttcagct
     6061 tctgcttgta cagattctaa aatttcatta cgacgttttg ttgctaaatc aatgatattt
     6121 tgtgcttctt ctttcgctct aaggatttct tgatccgcag ccgctttaga atcagcttgt
     6181 tcttgtttcg ctttttcggc agaagccaat gcgctagcaa tatttgcctg acgctcttct
     6241 atcactcgaa ttagcggtgg ccatacaaac ttcatacaga acgcaacaaa aagtgcgaac
     6301 gcaatcagtt gaccaattag tgttgcattt aaattcacaa cgccctccta aaagccggta
     6361 aagttattca actgaataac ggttaatcaa atcccgactt atttcaataa atcaatgaac
     6421 gggtttgcga agatgaatag taatgaaata cctacagcga tcattgcgat agcatctaaa
     6481 agaccagcaa cgataaacat tttagtttgt aagcttgatg ctaattccgg ctgacgtgca
     6541 gatgactcta agaatttgcc acctaagata gcaaagccga tagcagtacc aagagcagcg
     6601 aaagctaata aaattgaagc accaataatt gttgctgtga ttactgattc cataatgttc
     6661 tccattagat tgaggaatta gcccgaaggc tgggttgttg ataaaaatac atatcaagcg
     6721 gtcaatattt tgcaaaaatt tgcgaaaaac ctaccgcttg taaggctagt ttaatgatcc
     6781 gctttgttat aagcgatact caaataaaca atcgttagca tcataaaaat aaaggcttga
     6841 agcgtaataa ctaaaatatg gaaaatcgcc caaaccaagt gtaatggaat ccccaacgcc
     6901 tgtagagcga agttgtctgc catatacatt actgcaataa ggataaagat taactcacct
     6961 gcatacatat taccgaataa acggaacgcc aatgaaatcg gttttgccaa aagcgttacc
     7021 gtttcaagaa taaagtttac cggaatgaat gcccagtggt taaacgggtg taacgtgtat
     7081 tctttcacta aaccgccaaa acctttcgat ttcaccgtgt aaaataaaat taacgcaaat
     7141 acacaaatag acatacccag tgttgcacta atatctgccg ttggtactgc acgcaaatag
     7201 tgaataccaa acagattggc taactgtggt aagaaatcga ccgggataag gtcgatcgca
     7261 ttcataataa acacccaaca gaaaatcgtt aatgctaatg gtgcaattac attgcgaggt
     7321 ccgtggaagt tatccttcac taaaccgttt acccactcaa taacaatctc aacaaaacat
     7381 tgcaattttc ccggaacgcc cgttgttgca ttttttgcta cacgcgagaa cacccataaa
     7441 aaaatcgcac ctgccactaa agagaagaac agtgtatcta aatgcactgc ccaaaagcct
     7501 tcacctgtcg ataaaaaact caagtgatgg ctaatatatt ctgctgtcgt tccagccata
     7561 acatatcctt ttaaattaaa attagtggtg atgtttgctt aatacaaacg ggatcatatt
     7621 atttaaaaat aatgcgataa aataacccac gaaaaacaac acaacatcta gagcagggat
     7681 aagtttaaaa cagccgatca ccaaaataat agtaaccaac catttcattc cttcaccacg
     7741 atagaaagcg gtcatttttg actgattttt tgcagatttc ttaaagaaaa cccaatatac
     7801 aaacacaaga tgaggcaaaa aactcgctaa agcaccgcct agaaaggaaa aattaaaatt
     7861 cattttccat aaagacactc caagtgcaaa aacgcacaag agaaaaaatt caacctttaa
     7921 tgcttgttta tattgtgatt ttgctctatt gattacagct gacacatttt atcctttttg
     7981 aataagtaaa ccacacaatc aggctaataa ttagccactg ttgaatcaaa gaaaatccgt
     8041 gctaaattat actttacaaa tgaattgttg caaccgaaaa gcgacacaaa aacttgttat
     8101 tcttcacatt tttgaaaaaa aattttattt acgttgcatt tttttgagta aaaccatcaa
     8161 aatcagttaa caaaaaaaca acaaatagag gtgtgtgtat ttttctctgt cacattattt
     8221 caatatttga taaattcccc ttgcttaaaa atgaataaaa attcataata attgcatata
     8281 ttttcacttc cgagtagaaa atgaaataaa aaaagaagaa agaaataggc aaaataacta
     8341 atttttactt gaatgcaaac gtttgcttga ttaaacttag tcgcctactt ctaaggctac
     8401 acagataaaa tttgatgatt aggagtaaaa caatgttcaa accagcaatt ctggttctca
     8461 ctgacggttc tgtcttttcc ggcaaatcca ttggtgctga cggtcacact atcggtgaag
     8521 tggtatttaa cactgcgatg accggttatc aagaaatcct taccgatcct tcttatacta
     8581 aacaaatagt caccctgact tacccccaca ttggcaatac cggcattaat ttagaagata
     8641 aagaatcccc caatgtttgt gcctccggct taattattcg tgatcttcct cttctccata
     8701 gcaacttccg tgcgtcctcc tcactttcag attatctcat tcaacaaaac attgtcgcta
     8761 ttgccgatat tgatacccgc cgtttaaccc gaattttacg cgaaaaaggg gcacaagccg
     8821 gttgtatttt agtaggcgat gccacagaag agaatattgc taacgctttg gcatttgcca
     8881 acagtttcgg ttctatggct gggaaagatt tggctcaaag tgtcacctgc aatgaattat
     8941 ataaatggac agatggtgaa tggcaattag gtaaaggtta tgtaccgcaa caacaaaccg
     9001 agttccacgt agttgcctat gattacggtg taaaacacaa cattttgcga atgcttgccg
     9061 aacgaggctg tcgcttaacc gttgtgccgg caaaaacctc tgctgaagct gtgcttgcgt
     9121 taaatccgga cggtattttc ctttctaacg gcccggggga cccggagccg tgcgattatg
     9181 ccattaccgc aatccgaaaa ttattggaaa cgcaaaaacc cattttcggc atttgcttag
     9241 ggcatcaact tttaggttta gcggcaggcg gtaaaaccaa aaaaatggtg tttggtcatc
     9301 acggagctaa tcacccggta caagatttag acagccaaaa agtgctcatc accagccaaa
     9361 accacggttt tgaagtagat gaatccagct taccatctaa tgtgcgagtg actcaccgct
     9421 cactgtttga cggcacggtg caggggattg aactcactga tagaccggcg ttttcgttcc
     9481 aaggtcaccc ggaagccagc ccggggccga atgatgttgc ctatttattt gaccgatttg
     9541 tcagtgagat gaaaaaagtg aagtaaggac gtacctatgt gtgtccatca agcggtcgaa
     9601 atttgcaaaa aacttaccaa aaatgaccgc ttgtagcctg aaataaacga gataaaagag
     9661 agcccaaaat gccaaaaaga acagacataa acactatcct aattatcggt gccggtccga
     9721 ttgtgatcgg tcaagcgtgt gaatttgact actccggagc ccaagcgtgc aaagcgttgc
     9781 gtgaagaagg gtataaagtg gtgttggtga actccaaccc tgcaaccatt atgaccgacc
     9841 cgaatatggc agatgtgact tacattgagc cgattgaatg gaaaacggtt gaaaaaatca
     9901 ttgaaaaaga acgccctgat gccattttac cgactatggg cgggcagaca gcgttgaact
     9961 gtgccttaga tctttcgaaa aacggtgtgc tgaaaaaata taatgtagag ctgatcggag
    10021 ctaccgaaga cgcgattgat aaagccgaag atcgtggtcg ttttaaagaa gcaatgacta
    10081 aaatcggctt gaatacgcca aaatcattcg tttgccacag ttttgaagag gccttagcag
    10141 cacaagagca ggttggcttt ccaaccctga ttcgcccctc tttcacgatg ggaggctccg
    10201 gcggtggtat tgcttataat cgagatgaat ttatcgccat ctgcgaacgt ggttttgaag
    10261 cctcgcctac tcacgaatta ctgattgaac aatccgtact aggctggaaa gagtatgaaa
    10321 tggaagttgt gcgtgaccgt gccgataact gcattatcgt ctgctcgatt gaaaacttcg
    10381 acccgatggg tgtacacacc ggcgactcca ttaccgttgc tccggcacaa acgctaaccg
    10441 ataaagagta tcaaattatg cgtaacgctt cgctggcggt attgcgtgag atcggcgtgg
    10501 acaccggcgg ttcaaatgtg caatttgcga ttaaccctgc aaacggcgag atgatcgtca
    10561 ttgaaatgaa ccctcgtgtg agccgttctt ctgcactcgc atctaaagcc accggcttcc
    10621 cgattgccaa agttgcggcg aaattggctg taggctacac actcaacgaa ttgcgtaacg
    10681 acatcaccgg agggctgatt ccggcttcat tcgagccgac tttagattat gttgtgacca
    10741 aaattccacg cttcgccttt gaaaaatttg ccaatgcaga cgaccgatta accactcaaa
    10801 tgaaatcggt aggcgaagta atggcaatgg gaagaacctt ccaagagagt ttacaaaaag
    10861 ccttgcgagg cttggaaacc ggcatttgcg gctttaactt gctttccgaa gagcctgaaa
    10921 aaatccgccg tgaacttgcc aaccccggac caaaccgtat tttgtatgtt gccgatgcct
    10981 ttggagcagg cttttcattg gaagaagttc accactattc taaaatcgac ccgtggttct
    11041 tggtgcaaat tcaagactta gtacttgaag aattagcgtt agagaaaaaa cagtttagcg
    11101 atctagatgc taacgaaatc cgccgcttaa aacgcaaagg cttctcagac aaacgtatcg
    11161 cacagcttac caaagtcagc gaaaaaatgg tgcgggatta ccgccatact tacgctattc
    11221 taccggtgta taaacgggta gatacctgtg cagccgagtt tgcctcagat acggcttacc
    11281 tctattctac ttacgaggaa gagtgtgaag ccaaaccgtc tgatcgcaaa aaagtgatga
    11341 ttttaggcgg cggaccaaac cgtatcgggc aggggattga atttgactac tgctgcgttc
    11401 acgcctcgct tgctctgcgt gaggcaggtt atgaaaccat tatggtgaac tgcaaccctg
    11461 aaaccgtttc caccgatttc gacacctccg accgcctcta tttcgagccg ctaacattag
    11521 aagatgtact ggaaattgtg cgtgtagaaa acccgtgggg cgtgattgtg cattacggcg
    11581 gtcaaacacc actgaaatta gcgaatgcct tatctgatgc cggtgtaaac attatcggga
    11641 cttccgccga cagcattgat gccgccgaag accgtgaacg tttccaacaa attctaaaca
    11701 ctctcggttt aaaacagccg gcaaatcgca ccgcccgtaa tgcgttggaa gcggtggcat
    11761 tggcaaatga agtaggctat ccgctggtgg ttcgcccatc ttatgtgcta ggtggtcggg
    11821 caatgcaaat tgtgtataac gatgaagagc taaaccgcta tatgcgtgaa gcggtatcgg
    11881 tttccaacga cagcccgatt ttactcgacc acttcctcaa taatgcgatt gaagttgatg
    11941 tggattgcat ttgtgacggc gaaaatgtac tcattggcgg cattatgcaa cacgttgagc
    12001 aagccggtat tcacagtggc gatagtgcct gctcactacc tgcctattcg ttaagcggtc
    12061 atgttttgga cgaaattcgc aaacaaacca acgcaatggc gtttgctctc ggtgtgaaag
    12121 gcttgatgaa cgtacaattt gcagtacaaa atgaggtcgt ttatgtgttg gaagttaacc
    12181 ctcgagccag ccgtactgta ccgtttgtca gcaaagctac cggtcgccca ctggcaaaaa
    12241 tcgctgctcg cgtgatggct ggtgaaaccc ttgccgactt aaatgcatta gaggaaatta
    12301 tcccaagcaa attcttcgtt aaagaagcgg tattcccatt catcaaattc ccgggtgtgg
    12361 ataccattct cgggcctgaa atgcgttcta ccggcgaagt gatgggcata ggtaatacct
    12421 ttgctgaagc gttcttcaaa gcacaactgg gtgccggtga acgaattcca aaagtcggca
    12481 aagtgtttat gtcggttatt gaagagcata aacctgcttt aatttacatt gccaaacgtt
    12541 tccaagaaca aggctacggt ttatgtgcca ccttcggcac tgcaaacttc ctacggcaac
    12601 acggaattgc agtccaaacg gtaaacaaag ttcgtgaagg gcgaccgcat attgtcgatg
    12661 cgatcaaaaa cggcgaaatt gcgttggtaa tcaacaccgt cagcggcgag cctgaagtga
    12721 ttgccgacag ccactctatc cgccgtaccg ccttgcaaca acgtgtgcct atttacacca
    12781 cccttgcagg tgctgatgct atctcctacg gcgtaaaaca tatcaatgag tttgatgtgt
    12841 attcagtgca ggaattagaa caaaaggcta actaaaataa agctaaaatg acaagcggtc
    12901 agattttgca aattttttac aaattctgac cgcttgtgtt tgtagaggat tatttagtaa
    12961 atttatatgc taaatcattc cagcgtaatt catttttgag ttgtggtagc tcggtatttt
    13021 ggttgatatg gataaactca ataccaaaat attcagcgaa cattcgtagc atatcggcat
    13081 caatatctaa actaaataca ctatgatgag caccgccggc tagaatccat gcttgagtac
    13141 ctacataaaa atttggttga agcttccaaa aagcatgccc aactggtaaa tttggcattg
    13201 gatttggtga ggcgatagcg tctaaatccg caacaaccat tctaaaacgg tctcccatat
    13261 ctacaacggt tgcattcact gcttttcccg ccttactact gaaaattaag cgaacaggat
    13321 ctgccttacc gccaatacct agcggtcgaa tggctaatgt tgatttctct ttggcaatag
    13381 acggacaaac ttcaagcata tgagccccca ataccacttg gttttgttca tctaaatcat
    13441 aggtgtagtc ttccataaag gaagaaccgt ttggtaagcc ataacccatc actttcactt
    13501 cacgcacaag agctgcggtt ttccaacctc cttcaccacc aaatccgtag ccttgttcca
    13561 ttaaacgctg aacagccaag cccggtaatt gtttaatccc atttagattt tggaaattat
    13621 cagtaaaagc tttaaatcca ccattatcta agaatgtttt taagccgatt tcaatacgag
    13681 cagaatatgc gagagattct cttgctgaac cattttcttg caagctatcc tcaacattat
    13741 acaagcggtc agattttgca aaaaaatgac cgcttgttgc catttctaaa gtgtgatcta
    13801 cctctcaaat ttacaaaatt ttttataaca tattgattta aaatgttttt tatatttttc
    13861 ctttaattga ctttaatttg tcttaagagt aacttataaa acgtgatttt actatttgaa
    13921 atattgaggt attcaagatg actattcgag tttcctatga agaattaaaa gctcaattta
    13981 aacgtgttct gttggctcgc aatgtgcgtg aagagattgc cgaagagtgt gccactgtgt
    14041 ttgccgacac cactcaagcg ggtgcttatt cacacggggt aaaccgtttc ccacgcttta
    14101 ttcagcagtt agagaacggt gatattgtgc cggaagccgt gccgactaaa gtgctttcac
    14161 tgggggcgat tgagcagtgg gacgctcacc aagccatcgg taacttaacc gcaaaaaaaa
    14221 tgatggacag agcaatggag cttgcatctg aaaacggcat cggcgtagtg gcgttacgta
    14281 atgcaaacca ctggatgcgt ggcggttctt acggatggca ggcggcagag aaaggctata
    14341 tcgggatctg ctggacgaac gcccttgcgg taatgccgcc ttggggggcg aaagagtgcc
    14401 gtatcgggac taacccgtta atcattgcgg tgccgactaa cccaatcacg atggtggata
    14461 tgtcttgctc aatgtactca tacggaatgt tggaagtgca tcgccttgct ggtcgtcaaa
    14521 cctttgtcga tgctggtttt gatgatgacg gcaacccaac ccgtgatccg gcaacggtcg
    14581 agaaaaacct ccgtctaatg ccgatgggct tctggaaagg ttcgggctta tcgattgtgt
    14641 tagatatgat cgccaccttg ctctctaacg gtgaatccac tgcggcagtc accgaagata
    14701 aagacgatga atattgcgtg tcgcaagttt atatcgctat tgaagtggat cgcttaattg
    14761 acggcaaaac caaagacgaa aaactcaacc gtattatgga atatgtgaaa accgcagaac
    14821 gtgccgaccc tgatgtggca gtacgtttac cgggccacga gttcacccaa attctagccg
    14881 ataacaaagc caacggcatt ccggtggatg acaccgtttg ggcgaaatta caggcgttgt
    14941 aattgcaaat tgaccggtag ggtgggcgta agcccacgca ttagacacaa tcataagatg
    15001 acgtgggctt acgcccaccc tacaagcggt catttttccg cagaatttta caaaacggag
    15061 gtatgaatgt tttttggtca tatttcacaa gttaaagcga atcgataccc gccggcgatc
    15121 caaattgcgt tgaactatct gaaaaatacc gattttgatg cgatggaagc aggggtttac
    15181 gaactcaaag gcagacaaat ttatgtgcag gtgttggatt tgaacaccaa atcgaaacac
    15241 gaatttcagc cggaagtgca tcgcaattac ctcgatgtgc agtatctaca ccgaggcaaa
    15301 gagataatgg ctgcggcggt ggataccggc accaatccga ttgcgatgga atacaaccct
    15361 gagcgggata ttcaatatta tcaatccgtt gccaacgaaa atgaatttcg ctgcgttgaa
    15421 ggcaattttt gacggcacgg aaaccattcg caaagtggtg gtaaaaattg cgatgagcga
    15481 aattgaggag caacgatgaa agccttagct caattagtcg gaaaactgtt agaaatcttg
    15541 gtggtggtga ttttatccgc aatgtcgatt ttggttttcg tcaatgtggt actacgttac
    15601 ggttttaaca gcagcattag cgtgaccgaa gaggtgtctc gctatatgtt tgtttggttg
    15661 gcgtttttag gggcggtgtt ggcatttagc gaaaatcaac acgttaaagt cacaatgctg
    15721 accgataaat tatcgccaac ggctcgtaat atcttaagcg tagtcacaga tttagcgatg
    15781 ttgttctgtt gctacttaat tgtggacggt agctggattc aatttaacct caacctgaat
    15841 aactttgccc cgatttcagg cttaccgcaa ggcatcactt tccttgccag cacagtggca
    15901 ggggtattaa tcggtttatt aattattgcc cgcattgtgg caacggttgg cgtgattgca
    15961 aaaggagaaa aatagatgac tgtggtaatt tttctttccg tcttgcttgg ctcgatttta
    16021 ttgggcattc cggtagcttt cgccctgttg gtctgcggtg tggcgttaat gttgcattta
    16081 gatctgttcg atgcccaaat tttggctcaa caaattgtga gcggtgcaga cagtttctcg
    16141 ctaatggcaa ttccgttctt cattcttgcc ggtgaaatta tgaacgaggg cggtctctcc
    16201 aaacggatta tcgacctgcc gatgaaatta gtcggtcata aacggggcgg tttaggtttt
    16261 gtagcaattt tagcggcgat gattatggca agcctttccg gttcggcggt ggcggatacc
    16321 gcagcggtag tcgcaatgct actgccgatg atgaaaacca ccggttatcc gattgaccgc
    16381 tctgccggtt taatcggtac ggcagggatt atcgccccaa tcattccgcc aagcattccg
    16441 tttatcgtat ttggtgtggc tagtggcgtg tccattacca aactgttctt agccggtatt
    16501 ttcccgggca ttatgatggg gatctgcttg gctgcccttt ggtggtggca ggcaaaacgt
    16561 ttggatttaa tgaccttctc caaagcgacc aaagaagaac tttgcatttc atttaaaaac
    16621 agtatttggg cgttattgtt gcctgtcatt attatcggtg gtttccgctc gggaatgttc
    16681 accccaaccg aagcgggtgc ggtcgcaact ttctatgcct tgattgtttc actgtttgtt
    16741 taccgtgaaa tgaagttcaa agatttatac aaggtagtgt tggcggcagc gaaaaccacg
    16801 gcagtagtga tgttcttagt ggcagcggca aatgtgaccg gctggctgat taccgtagcg
    16861 gaattaccga tgatgctcac cgagctgtta gagccgttaa ttgaaagccc gaccacactc
    16921 ttattggtga ttatgttagc ggtgtttatt atcggaatgg tgatggactt aaccccaacc
    16981 gtgctgattt taacgccggt attaatgccg ttggtggaag aggcaggcat tgacccggtt
    17041 tacttcggtg tgctgtttat tcttaacacc tccatcggct tgattacacc accggtcggc
    17101 aacgtgctaa acgtgattac cggcgtgtcg aaactgccgt tcgaccaagc ggctaagggc
    17161 gtaatgcctt acctcgtaat gatgatttgt ttactactgt tgttcgtgtt cttcccgtca
    17221 ctggtgttag tgccgttaag ctggatgcag taatggtttg aaccgaaaca agcggtcgaa
    17281 ttgacgcaaa aatttgcaaa tttttttggc aaaacagacc gcttgcaact caatttttaa
    17341 tcacaggaga accctatgaa attatttaat ttaaaaacct tatttgcctt agtggcaggc
    17401 gttgcggtaa tgagcggctc tgcaatggca gaaacctcac tgcgttttgg ttatgaagcc
    17461 ccacgttccg atacccaaca catcgccgcc aaaaaattcg atgaattatt ggcagcaaaa
    17521 accaaaggtg aaattaaatt aaaactgttt ccggacagca ccttaggtaa tgctcaaacg
    17581 atgatcagcg gcgtgcgtgg cggtactatc gacattgaaa tgtccggctc gccaaacttt
    17641 accggcttag agccaaaact aaatgtgatt gatattccgt ttattttcaa agatcgtgaa
    17701 cacgtttaca aagtattaga cggcgaagtt ggtcaaggct tattaaaaga tttagaagcc
    17761 caaggcttaa aaggcttagc cttctgggac gttggcttcc gtgctttctc caactcaaaa
    17821 caccctgtga ccaagccgga agacatcaaa ggcttaaaag tccgcaccaa ccaaaacccg
    17881 atgtacattc aagccttcac cttactaggc ggcaacccgg tgccaatgcc actttctgag
    17941 ctttacaccg cactcgaaac ccgtgccgtt gatgcccaag aacacccaat cggcattttc
    18001 tggtcggcaa aactgtttga agtacaaaaa catttaagtt taaccaacca cggctacacc
    18061 ccgttaatcg tggtgatgaa caaagcgaag tttgacggct tagctcctga attacaaacc
    18121 gcattagttg aagccgcaaa agaagccggt caattccaac gtgatctcaa cgtgaaaaac
    18181 gaaaaagaga tcatcgaaaa attacgcaaa caaggcatcg aagtgatcga acaagtggat
    18241 aacgccccgt tcaaagcagc gattgaagcg gaagtccgca aagctttcgt ggaaaaacac
    18301 ggcgatgaat tattgaaaaa agtggatgcg ttagcacagt aattgacttt attttagtct
    18361 aaagctccct ccgtttgggg ggagttgttg tatagactct actataggag agactatgaa
    18421 actgtttaat ttaaaaactt tagccgcttt cgttgccggt tttgcactgt tttcaaccgc
    18481 acaagcagaa acctcattcc gctttgcgta tgaagctcct cgttctgata ctcaacatat
    18541 tgcagcgaaa aaatttaatg atttgctgca agaaaaatcc caaaaagggc tgaaattaag
    18601 cctcttccct gatagcacgc tcggcaacgc acaaacggcg attagcggtg tgcgtggcgg
    18661 tactattgat attgcaatgt ccagctcctc taactttacc gggttagaac caaaactgaa
    18721 tgtgattgat attccgttta ttttcaaaga cagagaccac gcctataaag ccttagatgg
    18781 agagattggt caaggtttat taaccgcatt agaggctcac ggtttgaaag ggttggcgtt
    18841 ttgggaagtt ggtttccgag cgttttccaa ctccaagcac ccgatcacca aaccggagga
    18901 tattaaaggc ttaaaaatcc gtactaacca aaacccgatg tatattcaag ccttctctat
    18961 tttaggtgca aatccggtgc caatgccgct ttccaagctt tacactgcac tggaaaccaa
    19021 agcggttgat gcccaagaac acccaatcgg cattttctgg tcggcaaaac tctatgaagt
    19081 acaaaaatat ttcagcttta ctaaccacgg ttacaccccg ttaattgtgg cgattaacaa
    19141 agcgaaattt gatgcactgc cagccgagca acaaaccgca ttagttgaag cggcaaaaga
    19201 agccggaaaa taccaacgcc aacttaacct agaaaatgag cagaaaatta tcgactcgct
    19261 caaaaaagca ggtattgaat ttgtggataa cttagatacc acaccgttca aagatgccgt
    19321 atcggctgaa acccgcaagg catttatcga gaaaaacggt gatggcttag ttaaagccat
    19381 tgatgcattg gcgaagtaaa aatagagcta caaagtgctc ggttaaacta acctcacacg
    19441 aatcgtgtga ggtacaagcg gtcaaaatcc gcaaaatttt tacacatctt tcctcaaagg
    19501 aggaacaaat ggcacagccc attttaagta tgcagcacat catcaaacgc tttggtagcc
    19561 atacggcggt gaatgatgtc agccttgata tccactcagg cgagatcgtt gccttactcg
    19621 gtgaaaacgg tgcaggtaaa tcgaccctaa tcaaaatcct tgccggtatt tatgaccgtg
    19681 acgagggtga tattcttttc cataaccaaa aaatccaatc tgcccacgct attgagaaag
    19741 gcaacaaaaa gcccatcgct tttatccacc aagatttagg cctaatagaa tggatgacga
    19801 ttgccgaaaa tatggcattt gtaatggggt tccctcgcaa atttggcttg attgactggg
    19861 cgaaagtgcg agagcagtcg caaaaagccc ttgattttgt aggcattaaa ctctctgccg
    19921 acactcgagt gtttgattta agccgtaccg aaaaagcctt actcgccatt gcccgtgcga
    19981 ttgccgtcaa tgcagaaatc ttggttttag acgaacctac tgcttcccta cctgcctctg
    20041 atgtagagca tcttttcact gtgttaaacc gcttacgttc ccaaggtgtg ggaatgattt
    20101 atgtgaccca ccgtttagat gaagtgatcg ccatttccga ccgcattttg gtgatgcggg
    20161 acggtttccc ggttgcagaa gggcaaacca agctctatga cgtcaaaggc ttggtcaaag
    20221 cgattgtagg cgaagagagc cgtggcagac agcgggtgga tttaccaacc gatactcctg
    20281 aaattttaag cctgcaaaat gtcgtggttg gcgacaccgg cccggtgaat ttccgtctgc
    20341 acaaaggtga gatgattgcc cttgccggct tgcgtggagc agggcaggaa gaaatcggcc
    20401 gcttattatt tggggagcga accttagaca aaggcgaaat tcagttagac aagcggtcat
    20461 attctgctaa agatccgcaa gatgcgatag ccaaaggtgt ggcattggtt gcgggagacc
    20521 gcacccgtga aagcgtgttt atgtcaatga ctaccagtga gaacctgttt atcaacccgg
    20581 tactaagcgg tcattcacca ttaaaacgct actcccgccg tgaagagtgg ggtgagagct
    20641 ggtacaaatt ccaactattc gatattcgtc cgaaaaattt gtttattgat gccagtgcgt
    20701 tatccggtgg taatcagcag aaaatcgtgt tggcacgttg gatgcacctc aatacgcctg
    20761 ttctgatttt agaagacccg actgccggcg tggatgtagg agcaagggca gagatttacg
    20821 acttgctcaa caaagccctt gcagaagggg tcgcaattat tgtgatttcc accgactttg
    20881 aagagatcgc tcatttgtgt aaccgagcat tagtcttcaa ccgaggggaa attgccggtg
    20941 aactactgaa cgaagaggtg actttcgcca atttgctggc gttggcatcg gatagtcagt
    21001 cgaaaaaaca ataacttact ctcgcccgct tgcgggagag agacagctaa aaatagcgtc
    21061 aggctatttt tagcagagag agggtaaaca agcggtcatt tttcttaaaa aatattgcga
    21121 accctctccc tccgaaagcc taacggcttt ctcctccctc tcccgcaagc gggcgagggg
    21181 aaaatgcaga attttaacgg agtaatttat ggctcaaaca aacatcaaat caaccgcact
    21241 ggaacaaccg gtatctatcg cccgtgacgg ttttatggca tggttttcac aaatgtccac
    21301 tcgttatggc ttactgtggt tatgtgtgtt attggtgatt attttctcac ttaccaccga
    21361 atctttcgcc tcaatgctga cattaaatgc gattttagaa agtaaatcga aaattgccct
    21421 acttgctttg gcagccacaa ctacaatgat tgtcggcaaa atcgacctga acgtgggttt
    21481 cggtattgtg ttgtggcaca ttcttgtgat tactctgcaa gtccaatacg gcttctcgtg
    21541 gtatatggcg atcttaatcg tgttagtgat tgccgctctt tacggcttgc tgaatgggat
    21601 tttagttgct cttgctgata tcgatagctt tgtggcaaca cttggctcag gcacagtgct
    21661 ttatgccgta gcactctggc actcgggcgg tcgccaaatt gtcggggatt tacccgatga
    21721 atttatcgca ctcaacagca cagaaatttt cggtatcccg atttctgctt tctatgtgct
    21781 ggcagtggct atcgtaatgt ggttggtgac agaacatacc ccgaccggtc gttgtatgta
    21841 tgcggttggt ggcaacccaa cggcagctcg cctaaatggt atcaatatta aaaaatacac
    21901 cattgtgccg tttattgtct ctagtgtaat cactgctttc acaggcgtat tgatctctgc
    21961 tcaacaaggc gttggtcagg caagtgtcgg tatggactac ttactgcccg cattggttgg
    22021 ggcattctta gggagtacca ctattcgccc agggcgtatt aacgtatggg gaacagtggt
    22081 ggggattgcc attcttgcca tcggtatttc aggtattcaa caattcggtg gagcgttctg
    22141 ggttgagcca ctctttaacg gtgcaacact gttactttcc atcactattg caggctatgc
    22201 ccaacgcaaa cgcttgttaa atcaaaaagc tgtacagaaa aaatcgacca actaagagga
    22261 tttgattatg aaaaaagcat ttcgtacaac cttaactgca atcgcaacct taactgctat
    22321 tgctttcgga aataatgcgt ttgccaatga cgacccgttc gtacaagaag caaaacagct
    22381 tgtcgcagca gctacggcta agcaagaaaa atgggacgga ccgacaaccg gccctcaatt
    22441 acaaaaagag aaaactgtga tttttattgc ctctgatatg aaaaacggtg gtgtacttgg
    22501 tgtgattgac ggaatgaaag aagctgttgg cgtaacgggc tggaagttag atgtattaga
    22561 cggtgcaggt acggtaaaca accaactcgc cgcacttaac caagcgattg cccgcaaacc
    22621 ggatgcgatt gtgattggtg gctggaaccc gaatgtggcg aaaatcccac tacaaaaagc
    22681 gactaaaaac ggcattaaac ttgcagcttg gcacgcaaca ccggagccgg gtgcgattga
    22741 taaatacggc attttctaca acgtgacctc tgactcagac gaaattgcac gcttatcggc
    22801 aatgcttgcg gtggcagaat ctaacggtaa ggcaaaagcg attattttaa ccgactcgct
    22861 atatgaaatt gcgttacgca aagcgaacgt catgaaagaa gttatcgaaa aatgtaaaga
    22921 gtgtaaggtg ttagagttta tcgacacgcc attggcggat acctcaagcc gtatgcctaa
    22981 cctcactttc agcttactgc aaaaatatgg cgacgattta gagtatgcgt tggcaattaa
    23041 cgatctctac tttgacttta tggctccgtc tttacgcacc gctggtaaag ataaaccgtt
    23101 tagcatttcc gcaggtgatg gctcagtaac ggcttatcaa cgtattcgca atggtgataa
    23161 acagtttgca accgtccctg agccacttaa cttacacggc tggcagttag tcgatgagct
    23221 aaaccgtgct ttcgctaacg aaaaaccgtc tggttatatc acaccggctc acctagtgac
    23281 taaagaaaat gtggcgtttg acggtggtga aaacaacctg tatgccccac aaaacggtta
    23341 caaaggcgct tacaaagcga tttggggcgt gaagtaatga cgtaggggca aataaatatt
    23401 tgcctcgaac aagcggttgt tttttgcaaa gattttgcga aatttaaccg cttgtcataa
    23461 aaaatagaga gggtaataca aatgagcaat aaatttatcc ccaaaatctc caaccctcgt
    23521 tttaatgagc tggttgggaa tacgtttgaa cttgaagagc tttttgataa ggcaatctgg
    23581 gcagaagggc cggcgtggag caagccacaa caagccctct attttagtga tgtaaaaggt
    23641 tgcacaatgt accgctggac agagcaagat ggtacacagg ttttccgcca gccgtcttat
    23701 ttttctaacg gcaatgcgtt tgatgcaaac gacaacttga ttacctgtga acacggcaat
    23761 cgaggtatca gtttaaccac gccaagcggt gaatatcgtt ttttggtcgg tcaattagac
    23821 ggaaaacgac tgaactcacc caatgatgtg gtggtaaaat cagacggcat catttggttc
    23881 actgatccac cttacggtat tttgtcggat aacgaaggca aacagtcgcc aagcgaaatt
    23941 atcggttgtt atatttactg ctatgatccg aaaaccgaca agctgaatat tgcaactttc
    24001 aacacgatgc gaccaaacgg cttggccttc tcgtcagacg aaaaacaact ctttgtggca
    24061 gatatgtcga gcgtggaatt tgacgaagat ggcttacacc acttagtcgt atttgatgtg
    24121 gacggtaata aactaaaaaa tcgccgagat gtggcggaaa ttagtcccgg cattccggac
    24181 ggcttccgaa ttaaacaaaa cggcgtaatt ttctgctctt gtgaagaggg aattattgct
    24241 cttttacctg acggcacgga gctgggacga tttgtgctgg gtaagctaac ctcaaattgt
    24301 agctttggcg atgatgaaca aacactgttt attacttgtt cgaatagcgt ttatcgttta
    24361 acaattaaat agcagaatgc attaagtgcg tctgttgtag cattacaagc ggtttctttt
    24421 tcaaaatttt ttacaaacaa ataatggata acaaaatgaa ctacacactc ggcattgatt
    24481 gcggtgggac ttttatcaaa gccgctcttt ttgatacaca aggtaatatt caagcccttc
    24541 atcgtgaaaa cgtttcggta atcagtgaac aggcaggtta tgcagagcgt gatatgtcac
    24601 aactctgggc tatttgtgcg gaagtagtgc gttcaaccat tgaaaaaagt ggggtaaacc
    24661 cagcggatat taaagcagta gggatttccg ctcaaggcaa aggggcgttt ttactggata
    24721 agcagaatcg accgttgggg cgtgcgattt tatcttccga ccaacgttcc cttgagattg
    24781 ttaaacaatg gcaggctgaa ggcattccgc aacaacttta cccgattacc cgccaaactc
    24841 tttggacagg acaccctgtg tcgattttga agtgggtgca gcaaaatgaa ccggagcgtt
    24901 atgcacaaat cggggcggtg ttgatgtcgc acgactattt gcgtttttgc ttaaccggtg
    24961 agttgcattg cgaagagacc aacatttccg aaagcaatct ttacaatatg gaaaaaggcg
    25021 aatacgacca acagttagcc gatttgttgg gaatgccgga tgtgttggag aaattaccac
    25081 cagtgattga accgaataaa atttcgggat atgtgactga aaatgccgcc aaattaaccg
    25141 gtttagcggt gggtacgccg atagtcggtg gcttgtttga tgtggtttct accgcacgtt
    25201 gtgccaattt agatgacgaa accaaactga atgcagtgct gggaacgtgg tcggtggtga
    25261 gcggcattac cgatcatatt gatgcaagcc aatcattacc ttttgtttat gggcgttatg
    25321 ccgaggcagg gaaatttatc gtccacgaag caagcccgac ttctgccggt aatttagagt
    25381 ggtttgtgaa tcagtggaaa ctcgattatg tgaaaatcaa ccaagctgtt gcccaattac
    25441 cgccggcggc aagctcggtg ctgtttgtgc cgtttctgta tggctcaaat gcaggcttag
    25501 gaatgcaagc cggtttctat ggtatgcaag cccaccatac ccaagcccat ctcttgcagg
    25561 caatttacga aggagtgttg tttagcttga tgagccattt aaaccgtatg tttatccgct
    25621 ttccaaaggc tgacgtgctg cgagttaccg gcggaccggt gaaatctaaa gtttggatgc
    25681 aaatgttggc cgatttaacc ggaatgcggc ttgaaatacc acaaatggaa gaaaccggct
    25741 gcttgggtgc ggcattaatg gcaatgcaag gggttgggct ggatataagc caagttcaag
    25801 ccctaaataa tgaaatgcag gtgtttgagc caaatccgga aaattatgcg gcttatcaga
    25861 cgaaatatca gcgttaccaa aaat
//
LOCUS       DANLXF010000005        23713 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.005, whole genome shotgun sequence.
ACCESSION   DANLXF010000005 DANLXF010000000
VERSION     DANLXF010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 23713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23713
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.005"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            6..1196
                     /gene="malE"
                     /locus_tag="PXD66_000127"
     CDS             6..1196
                     /gene="malE"
                     /locus_tag="PXD66_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005537801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HDL6318468.1"
                     /translation="MKNKCVKFTLATLATLVLSGTVMAKMTEGKLVIWINGDKGYNGL
                     AEVGKKFEKDTGVQVLVEHPDRLEEKFAQVASTGDGPDIMFWAHDRFGGYAQSGLLAE
                     VNVSKEFKDKFVDFAWDAETYNGKIIGYPVAIEAISLIYNKDLVKEAPKSWEEIIELD
                     KKLKKEGKNAIMWNLSEPYFTWPIAASNGAYAFKFANGKYDPKDIGVNNEGAVKALQF
                     VVDMVKNKHISADMDYAVAEASFNKGQSALTINGPWSWGNIDKSGIKYGVTLLPTLNG
                     QASKPFVGVLSAGVNSSSPNKDLAKEFLENYLLTDDGLDAVNKDKPLGAVALKSYQEK
                     LAADPRIAATMANAKNGEIMPNIPQMSSFWYAEKSAINNAVTGRQSVKEALDDAHSRI
                     QKQQ"
     gene            1315..2853
                     /gene="malF"
                     /locus_tag="PXD66_000128"
     CDS             1315..2853
                     /gene="malF"
                     /locus_tag="PXD66_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HDL6318469.1"
                     /translation="MLTQTQSKSTHWLKYLLAGLVLLLDFYLVVLMYSQGEYLFAILT
                     LIILTSGVYIFTNKNTYAWRYVYPGITGMAIFILFPLVATIAIAFTNYSGSNQLSFER
                     AVSVLTEQRYFAGDKYQFTLYPQADNKYQIALTNPTTEQTFVSEPISLATGTNVVVTE
                     QNRPTGEVAPLKVITQNRAALQSMKVVLPNNNELTMSSLRQFSEQKPRYQFDEENKIL
                     LNNETGKRYQANDETGFFQSIDENGHWQSETLEPGYTVTSGFNNFIKIFTDEGIQKPF
                     VQIFIWTVIFSLLTVVFTVILGMVLACLVQWEALKGKAVYRVLLILPYAVPSFISILI
                     FKGLFNQSFGEINMILNQLFGISPEWFNDPLLAKVMILIVNTWLGYPYMMILCMGLLK
                     AIPADLYEASAMDGASTWQNFSKITFPLLLKPLTPLMIASFAFNFNNFVLIQLLTNGR
                     PDMIGTTTPAGYTDLLVSYTYRIAFEGSGTQDFGLAAAIATIIFLLVGGLALLNIKAT
                     KMEL"
     gene            2906..3796
                     /gene="malG"
                     /locus_tag="PXD66_000129"
     CDS             2906..3796
                     /gene="malG"
                     /locus_tag="PXD66_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HDL6318470.1"
                     /translation="MAIVQSKSVRYRVWATHFILICFLALIIFPLLMVIGISLRPGNL
                     AIGDIIPSQISWEHWQAALGFDVTHADGTVTPPPFPVLRWLWNSIKVATITSIGIVTL
                     STTCAYAFARMKFKGKKTILQGMLIFQMFPAVLSLVALYALFDRLGQYVPFLGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDGSLEEAASLDGATPWQAFRLILLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLYPQNYLWGDFAAAAVLSAIPITL
                     VFLLAQRWLIGGLTAGGVKG"
     gene            3871..5928
                     /locus_tag="PXD66_000130"
     CDS             3871..5928
                     /locus_tag="PXD66_000130"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HDL6318471.1"
                     /translation="MKNALPYLCLFYTSSLFAQSWQHSHFSDFNDDLEKHFFQSQVQL
                     EKGSYPLRFKFGEQCYQPQQAIKLNQSVALVPCINEAPQLRLFRQGNYLAQIDLRSGT
                     PTPTISVEQQLQHNEAMFQSCPNWDKKPIEIDVSSTFAEGESVSDFYSGNTAIVKNGK
                     VTLMPNENASGLILLEKSNTENTATFDWKNATVYFVLTDRFYNGDPTNDHSYHRQKDG
                     MQEIGTFHGGDLKGLTEKLDYLQQLGVNVLWISSPLEQMHGWVGGGDKGDFPHYGYHG
                     YYHLDWTKLDANMGTEDDLSRFVQQAHQRGIRVLFDVVMNHTGYATLADMQEFNFGGF
                     YLTPDEISRTLGEKWTHWKPKSGQNWHSFNDFIRFNDNQAWQNWWGKEWVRADIADYD
                     SPKFDDLKMSLAALPDLKTESEQAVKLPEFFANKNTNAKTLPNAKVRDYLIGWLSDWV
                     RKYGIDGFRVDTAKHVEKSTWLVLKQSAQQALNEWQKANPNAGFNDRFWMTGEAWGHG
                     VFKSDYYQNGFDAMINFDFQDQAKQGLNCFAHIEPIYAEMNRKLSDFNVLSYLSSHDT
                     RLFFHSDSEQNIEKHKTAANLLLLSPGAVQIYYGDESGRTFGATGSDLIQGTRSDMNW
                     QELQQDPQKQALHQHWQKLTQFRQRHPAIGAGVHQLIKNDSYFAFKRVLNEDKVMVVW
                     AGN"
     gene            complement(6042..8768)
                     /gene="malT"
                     /locus_tag="PXD66_000131"
     CDS             complement(6042..8768)
                     /gene="malT"
                     /locus_tag="PXD66_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HDL6318472.1"
                     /translation="MLIPSKLVCSFRLQNSIPRKRLIQELDKSCSYPVVLINAPAGYG
                     KTTLVSQWIENKKNIGWYALDEGDNNSDRFATYFSAALHSAINEEIDVLLEENRKANL
                     LALFNQLLIKAAAFSEHFYLAIDDYHIIENDEIHEALKYWIKHQPSNMTLILISRSIP
                     PLSIASLRVQEQLLEIDINQLMFDHQESTAFFQARLGSELNQQDVIALCNEVEGWPTA
                     LQLISLFAKNKSHTLQVPLQDIAKRLAKSNHFHINEYLADEVLNKVDEETRLFILRCS
                     VLHSMNESLVQAVTGEQNSRTKLESLEKQGLFLQQMANSKWQSSDDSWWKFHPLFASF
                     LNLCCQNELYAELPQLHQKAANAWLQLGYVTEALHHAMQLSDTGLLLNILSKHAWTVF
                     HQGELKLLEESLNCLDYAHLTEHTNLVLLKAWLVQSQHRHIEVGGILADFSRALTENK
                     IELSKTANAEFNVLRAQVAINSGDENTALQLASEALNDLSENAYYAHIVATSIIGEAH
                     HCHGNLAEALAMLQKAERMARQHHTYHNILWSQLQQSEILLAQGFSQAAYEMLDKASE
                     FVKENHLQKVPMYEFLLRSKGKILWEWYNLDKAESMAVAGMNALSKFEDKLQCLTLLT
                     KISLARGNLDNTARLLGEVEQLEHSHSYHHDWIANADQVRIFYWQMTNDVSAVRNWLI
                     QNPSPVSDKNHFTQVQWRNIARSRILLEEYHEAKAILDNLIETAEKFSLISDLNRALI
                     VRNRLYFLQGEKELAQQDLIQALKLTRQTNFISAFVIEGDVMAQQIRHLLQLNVLDEL
                     VLHKAQFILRNINQFYRQKFAHFDENFVNQLLKNPKVPELLKISPLTQREWQVLGLIY
                     SGYSNEQISDELQVAATTIKTHIRNLYQKIGVTNRNEAISYTKELLMLMGYH"
     gene            8932..11301
                     /gene="malP"
                     /locus_tag="PXD66_000132"
     CDS             8932..11301
                     /gene="malP"
                     /locus_tag="PXD66_000132"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HDL6318473.1"
                     /translation="MTFNSIVEKYCRYFDVTDPKHFTLQQWYQVVAEGSLELIYSQPA
                     TKPTESRHVNYLSMEFLIGRLTGNNLMNLGYYEQIRDYLKQYQVELVDVLEQERDPAL
                     GNGGLGRLAACFLDSMATVGQNATGYGLHYQYGLFKQSFKEGMQKEAADTWARDSYPW
                     HRFNPSKTRHIGFGGKIKHIQADQYEWQPKLTIQGKAFDLPIVGYKNNIIQPLRLWQA
                     DSDQSFDFDAFNEGKFLSADKTIVNAAALTQVLYPNDNHQAGQKLRLMQQYFHCACSV
                     ADILDRHISEGYSLEDLAKRQVIQLNDTHPTLAIPELMRVLLDEHQFTWEQAWDICSN
                     TFAYTNHTLLPEALEQWDQRLFKQLLPRHYQIVEKINDIFHDKVQAEFGDDAKIWEKL
                     AVLFDYRVRMANLCVVTCFRVNGVAEIHSDLLVTDLFPEYHKLFPTKFCNVTNGITPR
                     RWIRQANPQLSDLLDRTLKQDWTHNLELLKGVEQYADDTGFREEYQRIKRNNKVVLAN
                     EIHKTLGLNVNPNAIFDVQIKRFHEYKRQHLNLLNIIATYQSLKANPNQDYTPRLFLF
                     SGKAAPGYYLAKNIIHAINNVADIINNDKQVNDRLQVAFLPDYRVSLAEKIIPAADVS
                     EQISMAGKEASGTGNMKLALNGALTLGTLDGANVEIADMVGEENVFIFGHTVESVREL
                     LAKGYQPKAYYNQDPILKNAVDFLATGKASNGDKEAFKLMLDSLLERDPFLVFADFDS
                     YRQAQEKIGVAYLDQETWLRSAILNTARLGAFSSDRSIKDYQQRIWLNR"
     gene            11383..13461
                     /gene="malQ"
                     /locus_tag="PXD66_000133"
     CDS             11383..13461
                     /gene="malQ"
                     /locus_tag="PXD66_000133"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HDL6318474.1"
                     /translation="MKITAKQYYRAGIMPYFFDERGVKKTASEKIKHSLFKAFNGNTQ
                     ARETPIPTVNIFYQHQPCFLPFHLADKKHPLTGTWQLQLENSNQIISGKIKARGITLP
                     QDLPLGYHQLQLKTVDKTFNCTIIIAPERCYQPQAVIEQKRLWGTFLQLYTLKSAHNW
                     GIGDFGDLKDFIQQIAPYQADFIGLNPIHSLFPANPNSASPYSPSSRRWLNIAYIDIN
                     QLAEFQQSDEAQTWFHSAEIQQQLSELRTQDWLNYEQIIPLKLKGLRFAFAKFQQNMT
                     ACAAPAFIEFVQKGGESLQVQATFDALHQHLSAQFADQWGWDFWEKAYQDYHSAAVQQ
                     FRETHKTDIEFYLWLQFVADQQLAECDAICRQQNMRIGMYRDLAVGVSPNGAETWNDK
                     QLYCLNASVGAPPDVLGPQGQNWGLTPMNPHVLKQQAYRPFIELVRANMSHCGALRLD
                     HIMSLLRLWWIPKGDSAVNGAYIRYPVDDLIAILALESQRHQCLIIGEDLGTVPKEIV
                     SKLKNAGILSYKIFYFEFDEQGKSRDLQHYPYQAMTTLSTHDLPTINGYWRGYDFELG
                     EKFGVYPNSNILAILKQDRVNAKAKILQRLQEHNVEIAPEISEQLNSPVNTAFIHCLQ
                     TYVANVSSGLFGFQPEDWLGMTEPVNIPGTSTQYANWRRRLSVNLEEIFTDKDIQALL
                     KEVNLIRKNQ"
     gene            complement(13526..15187)
                     /locus_tag="PXD66_000134"
     CDS             complement(13526..15187)
                     /locus_tag="PXD66_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HDL6318475.1"
                     /translation="MSEIALTVSLLSLVAVLGLWIGHIKIRGVGLGIGGVLFGGLFVS
                     HFMAQFDVKLDAHTLHFIQEFGLILFVYSIGIQVGPGFFASLKHSGLKLNGFAVLIVL
                     ISGILVILIHKFFNVPLPVILGIFSGAVTNTPSLGAGQQVLAELSAESVTEIMGMSYA
                     IAYPFGIIGILLSMWLIRILFRINIEAEAEAFNKQQNRSKDQLSTLNVRLTNPNIHGL
                     TLKQIPDFNLHEVAYSRLKRGNELSIPRVDTILQIGDILHLVGEESSLRKMQIILGEA
                     VDVSVSTKGTIYRSERAVVTNEKVFGKQIRQLMLKGKYDVVISRLNRAGVELVPTGDM
                     TLQFGDVLNLVGRQADIEMVMTIIGNAHSKLQQVQMLPIFIGIGLGVLLGSVPIYLPG
                     FPVALKLGLAGGPLVVALILARIGSFSKLYWFMPPSANLALREIGIVLFLAVVGLKSG
                     GRFLETLLSSEGVEWICYGAIITFVPLMIAGLIARMYNKVNYLTICGLLAGSMTDPPA
                     LAFANEIKEGNGAAALSYATVYPLVMFLRIMLPQVLAIMLWVSGG"
     gene            15405..16781
                     /locus_tag="PXD66_000135"
     CDS             15405..16781
                     /locus_tag="PXD66_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HDL6318476.1"
                     /translation="MLSNEVFISIIVLLILSLLRINVVIALIISALTCGLIGNLGVEG
                     AGFTEALEKTIKSFTGGLGGGAETAMNYAVLGAFAVALSKSGVTDLLAYKVIKSFGHS
                     PSGRSVTWFKYIVLFVLVAFAMSSQNLIPIHIAFIPILIPPLLSVMNKLQIDRRAVAC
                     ALTFGLTATYMLIPAGFGQIFIESILVKNINQAGEPFQLVTTTAEMTKAMLIPVSGMI
                     LGLLFAFFVSYRKPRVYIENMPEPTADDIEARVANVKPFHIGISIVAIIATCSVQLAT
                     NSTIIGGLVGLVIFALGGVFRLKESNDVFQAGLRLMAMIGFVMIAASGFAGVVNSTGG
                     VPALVESLRDVISSKEMAALLMLVVGLFITMGIGSSFSTVPIITSIYVPLCISFGFSP
                     LATMAIVGVAAALGDAGSPASDSTLGPTSGLNADGKHDHIWDSVVPTFIHFNIPLLVF
                     GWIAAMTL"
     gene            16879..17682
                     /gene="xthA"
                     /locus_tag="PXD66_000136"
     CDS             16879..17682
                     /gene="xthA"
                     /locus_tag="PXD66_000136"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDL6318477.1"
                     /translation="MKFISFNINGLRARPHQLEAIIEKHQPDVLGLQEIKVADEDFPY
                     DLVNHLGYHVFHHGQKGHYGVALLTKKEPIAVRKGFPTDVLDAQKRMIMADIETDFGV
                     LTVLNGYFPQGENRSHETKFPAKQKFYADLQNYLESELKPENPIIIMGDMNISPTDLD
                     IGIGEPNRKRWLRDGKCSFLPEEREWLDRLYSYGLVDTFRAGNPDVNDKFSWFDYRSK
                     GFDDNRGLRIDLILASKSLAERCVETGIDLDIRAMEKPSDHSPVWATFK"
     gene            17836..18420
                     /gene="lptC"
                     /locus_tag="PXD66_000137"
     CDS             17836..18420
                     /gene="lptC"
                     /locus_tag="PXD66_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HDL6318478.1"
                     /translation="MNKRLTVILLIIVAVLGGWYYSLQEKENTGLDQLIKKEGQPEYV
                     GNKMSTTVYDLKGNPQYFAQATEIKRYESTERTEFFKPFIELFAKESTQKQWKVSADY
                     AEITKEKMLNLKGNIKLVALDPASRLQQITTDTLTVDLNTQDIFTESTVKSIGSGFTT
                     TGVGLKGNLKQQVATLQKDVKSYIEPTIIQETKE"
     gene            18444..18950
                     /gene="lptA"
                     /locus_tag="PXD66_000138"
     CDS             18444..18950
                     /gene="lptA"
                     /locus_tag="PXD66_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HDL6318479.1"
                     /translation="MKLMFKSILVSVLLGSSFSAYALKGDTDQPINIDSGSQSLDMTN
                     NIVTFSDNVVITQGSIKVTAANVKITRQEGKKETIDATGSPVTFQQTLDNGKPVNGKG
                     NSVHYDLNSEFLTLIGNAELKQQGSFIKANKITYDVKKQQLKATSGGKTRVKTVLIPN
                     ELQDNKKK"
     gene            18962..19687
                     /gene="lptB"
                     /locus_tag="PXD66_000139"
     CDS             18962..19687
                     /gene="lptB"
                     /locus_tag="PXD66_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005727816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HDL6318480.1"
                     /translation="MSVLYAEHLAKSYKQRQVVKDVSLNVKSGEIVGLLGPNGAGKTT
                     TFYMVVGLVRHDHGTIRIDDQDISTLPMHDRAKQGIGYLPQEASIFRRLSVYDNLMSV
                     LQVRKNINDKQRKERAEELITEFHIEHIRNSLGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKKIIVNLKERGLGVLITDHNVRETLDVCERAYIVGSGEMIAS
                     GTPQQVLENPDVKRVYLGDQFKL"
     gene            19729..20241
                     /locus_tag="PXD66_000140"
     CDS             19729..20241
                     /locus_tag="PXD66_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL6318481.1"
                     /translation="MTKLTEFLSPENVRQGVLISSKKRALELVGKIVAESINQTEQQS
                     EALCPIECFGNLFKREKLGTTGLNQGIALPHAKLPACGDIELTKPIAVFLQLEEAIDY
                     EAQDNKEVDLIYAMMFPENSCEQYKSCLPAIAQKLTDKSLLKQLRAAESAEDIWQILV
                     YADNHLKDEE"
     gene            20254..21117
                     /gene="rapZ"
                     /locus_tag="PXD66_000141"
     CDS             20254..21117
                     /gene="rapZ"
                     /locus_tag="PXD66_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDL6318482.1"
                     /translation="MELIIISGRSGSGKSVALRALEDAGYYCVDNIPLPLIPTLTYYL
                     TQENRSAVISLDIRNLPNTPNAIEELLTQLEPLNAKLIFLDCERNTLIRRYSDSRRIH
                     PLSTQDDLSLEGALDLEQKLLEPLVQNANYIIDTSHLSSHELAENLRQILQGSTEKEL
                     KIIFQSFGFKYGLPADSDYVFDVRFLPNPHWNPELRPMTGLEQPVIDFLERQTEVHNF
                     IYQTRNYLEMWLPMLEQNNRSYLTIAIGCTGGKHRSIFIAEQLAKYFQAKGKNVQVRH
                     KSLEKHHKKTS"
     gene            21200..22384
                     /locus_tag="PXD66_000142"
     CDS             21200..22384
                     /locus_tag="PXD66_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HDL6318483.1"
                     /translation="MKSADIVIVGGGMVGLALAGLLKDVDCQIKIIEKNTPKFDADTI
                     YHRVSAINASSQKILEQIGAFQHIPAQRLSPYTEMKVWEKDSFANIHFDNNDNEIKQL
                     GLSQLGFILENAVIQHALWQQVSQQANVEYIIAAPQTLGVSENGAFLTLDNGEMLSAK
                     LVVGADGANSWVRNQMKMPLTSKDYQHTALVCNVKTTEKHGKTARQIFAEDSIFAFLP
                     MADEHLCSIVWSLPPNKANELKNASEAEFNKALTIAFDNQLGLCEVQSLREIYPLAAR
                     FCRDFAQPRIALIGDAAHTIHPLAGLGVNLGFADARTLANEIQKQLVNNQDIGEYRHL
                     RQFERERKAQAMKLLATMEGLKQLFDGNHPLKKLFRGIGLSLTNQLPFVKKQLIQQAI
                     YL"
     gene            22516..23100
                     /locus_tag="PXD66_000143"
     CDS             22516..23100
                     /locus_tag="PXD66_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein HlpB"
                     /protein_id="HDL6318484.1"
                     /translation="MNKLTKISATALVALFLAACDKPAEKAPATEPTKTTEAAPTTTA
                     PAAEAAKPAETAVAAPSEAAKADYQKLVDWNTAQESTMATAQAELQKKLATQDPKQIQ
                     EGLSAFNKKVEETVKSLDAIEVSDAQIKTFKEKTKSVLTLSSEVISEQVKTISTPNDQ
                     AALQAVQQKAQSLIEAGNELQKLNVELQQRFSAK"
     gene            23167..>23713
                     /locus_tag="PXD66_000144"
     CDS             23167..>23713
                     /locus_tag="PXD66_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein Hlp"
                     /protein_id="HDL6318485.1"
                     /translation="MNTLAKISLSVLFTLFLTACEQPNSTKTQSSAESPVQVKEESKE
                     EVKPADTGAQDYKMLREWQDTQEKALNDAIKAATETLTDKQKADSTLMQETVNNALLA
                     QIDHIKISAETLNIQNNEVKALKDKTLEVLTLGAQMIVEGAKMEKNPTPEAHKAFGEL
                     QTKLNQLAEEGQQLENILRAKY"
BASE COUNT         7428 a         4872 c         4622 g         6791 t
ORIGIN      
        1 tttttatgaa aaacaaatgt gtcaaattca ccttagctac tcttgctacc ttagttcttt
       61 caggaacagt aatggcaaaa atgacggaag gtaaactcgt tatctggatt aacggtgata
      121 aagggtacaa tgggcttgcc gaagtcggta agaaatttga aaaagatacc ggtgttcaag
      181 ttttagttga acacccggat agacttgaag aaaaatttgc acaggttgcc tcaactggtg
      241 acggtccgga tattatgttc tgggctcacg accgctttgg tggttatgca caatccggtt
      301 tattggctga agtgaatgta agcaaagaat ttaaagataa atttgtcgat tttgcttggg
      361 atgctgaaac ttacaacggc aaaattatcg gttatccggt tgcgattgaa gcgatttcat
      421 taatttataa taaagattta gtgaaagaag caccaaaatc ttgggaagaa attattgagt
      481 tagataaaaa actcaaaaaa gagggcaaaa atgccattat gtggaactta tccgagccct
      541 atttcacgtg gccgattgcg gcttcaaacg gagcttatgc attcaaattt gctaacggca
      601 aatacgatcc aaaagatatt ggtgtgaaca atgaaggggc agtaaaagca ctacaatttg
      661 tagtcgatat ggtgaaaaat aaacatatta gtgcggatat ggactatgcc gttgccgaag
      721 cctcctttaa taaaggacaa agtgccttaa ccatcaatgg tccttggtct tggggcaata
      781 ttgataaaag cggtattaaa tatggtgtaa ctttactccc aacattaaac ggtcaagctt
      841 ctaaaccatt cgtaggggtg ttaagtgccg gtgtaaacag ctcaagccca aataaagatt
      901 tagccaaaga attcttagag aattatttac tcaccgacga cggtttagat gcagtcaata
      961 aagataaacc tttaggtgcg gtggcgttaa aatcttacca agagaaatta gcggctgatc
     1021 ctcgtattgc tgcaacgatg gcaaatgcga aaaacggtga aattatgcca aatattccgc
     1081 aaatgtcttc tttctggtat gccgaaaaat ctgctatcaa taatgcagtg accggtcgtc
     1141 aatcggtgaa agaagcatta gatgatgcac attcacgcat tcaaaaacaa caataattct
     1201 tcatcattct gagtgggtta ttatgaccca ctctattttt agattcaccc taaatacaat
     1261 aaaagtacag taaaagtacc gtaacaaaat gcagtaaaaa ttcgaggtgc tgttatgcta
     1321 actcaaactc aatccaaatc cactcattgg ctcaaatatc tcttagccgg attagtcttg
     1381 ctccttgact tctatcttgt tgtcttaatg tactcacaag gtgaatatct ttttgctatt
     1441 ttaacgctga ttattctaac ttccggtgtc tatattttta ccaataaaaa tacctatgct
     1501 tggcggtatg tttaccctgg cattaccgga atggcaattt ttattttgtt tccgctggtt
     1561 gcgactattg caattgcgtt taccaactat agtggttcta atcaattatc ctttgaacga
     1621 gcggtatccg tcttaactga acaacgctat tttgccggag ataaatatca attcaccctt
     1681 tatccgcaag cagataataa atatcaaatt gcattaacca atccgacgac agagcaaact
     1741 tttgtgtcag agccgatttc attggctacc ggaacgaatg tggttgtaac cgagcaaaac
     1801 agaccaactg gcgaagtcgc tccccttaaa gtgattacgc aaaatcgggc cgcacttcaa
     1861 tcaatgaaag tggttttacc gaataataat gaactgacaa tgagctcatt acgtcaattc
     1921 tccgagcaaa aaccacgcta tcaatttgat gaagaaaata aaatattact caataatgag
     1981 actggcaaac gttatcaagc aaatgacgaa accggtttct tccaatcgat tgatgaaaac
     2041 ggtcattggc aaagcgaaac acttgaacca ggttatactg tcacatcagg ctttaataac
     2101 ttcattaaaa tttttaccga tgaaggtatt caaaaacctt ttgtccaaat atttatttgg
     2161 acggtgatct tctctttatt aaccgttgtc tttaccgtta ttctcggtat ggttcttgcc
     2221 tgtttggtac aatgggaggc attaaaaggt aaagcagtct atcgtgtctt attgatttta
     2281 ccttatgccg taccatcatt tatttcgatt ctgattttta aaggactatt taaccaaagt
     2341 tttggtgaga tcaatatgat tttaaaccaa ctattcggca tcagccctga atggtttaac
     2401 gatccgctcc ttgccaaagt gatgatctta attgtcaata cgtggttagg ttatccttat
     2461 atgatgattt tatgtatggg gttactcaaa gctattccgg ctgatttata tgaagcctcc
     2521 gcaatggacg gtgcttccac ttggcaaaac ttcagtaaaa tcactttccc attactgtta
     2581 aaaccactta ctccgttaat gattgcttcg tttgcattta actttaataa ctttgtatta
     2641 attcaactat taacaaacgg tcgcccggat atgattggaa caaccactcc agccggttat
     2701 accgatttat tagtcagcta cacctatcgt attgcctttg aaggaagtgg tacgcaagat
     2761 ttcggcttag cagccgccat cgcaaccatt attttcttat tagtcggtgg cttggcactc
     2821 ttaaatatca aagcaaccaa aatggaatta taaaagcggg agggatttaa caatcccgac
     2881 cccaaactaa ctttaggagt tatatatggc aattgttcaa tctaaatctg tgcgttatcg
     2941 ggtatgggca acccatttca tcttaatctg tttccttgcc ttaattattt tccctttatt
     3001 aatggtgatt ggcatttcgt tacgaccggg aaatctggct atcggtgata ttattccaag
     3061 ccaaatttcg tgggaacatt ggcaagccgc tcttggtttt gatgttacac acgccgatgg
     3121 tacagtcaca ccgccgccat tcccggtgct acgctggtta tggaactcca tcaaagtggc
     3181 aacaattacc tctatcggca ttgtaacctt atctacgacc tgtgcttatg catttgcccg
     3241 aatgaaattt aaaggcaaaa aaaccattct acaaggtatg ctgattttcc aaatgttccc
     3301 ggcagtgctt tccttagttg ccttatatgc gttgtttgat cgtcttgggc agtatgtacc
     3361 gtttcttggt ttaaacacac acggtggtgt tatttttgcc tacttaggcg gaattgcctt
     3421 acacgtttgg acaatcaaag gctactttga aacgattgat ggttcattgg aagaagccgc
     3481 ttcccttgat ggtgcaaccc cgtggcaagc gttccgatta atcttattac cgctttcagt
     3541 gccgattttg gcggtagttt tcattctttc atttattgcc gccattaccg aagtaccggt
     3601 tgcctcactc ctattgcgtg atgtaaacag ctacacccta gccgttggta tgcaacaata
     3661 cctctatccg caaaactatt tatggggtga cttcgcagca gcggcggtct tatccgcaat
     3721 tccgattacg ctggtcttct tattggcaca acgctggtta atcggtggtt taactgccgg
     3781 cggcgtgaaa ggctagtttg caaaaaattc tctaaaaacg accgcttgtc gagctgatac
     3841 tgcggtcgtc aatctttatt agggctgtct atgaaaaatg cgttacctta tctctgctta
     3901 ttctatacct caagcctttt tgcccaatct tggcaacatt cccatttttc tgattttaac
     3961 gatgatctcg aaaaacattt ttttcaatcc caagttcaat tggaaaaagg gagctatccg
     4021 cttaggttca aatttggcga acaatgttat caaccccaac aagcgataaa attaaatcaa
     4081 tcggttgcgt tagtcccttg tataaatgaa gctccacaac tgcggttgtt tcgtcaaggc
     4141 aactatctgg cacaaattga tctgcgaagc ggcactccga cacccacaat cagcgtagaa
     4201 cagcagctac agcacaatga agcgatgttt caaagttgcc cgaattggga taaaaaaccg
     4261 attgaaattg atgtttcttc cacttttgcc gaaggcgaat cggttagcga tttttattca
     4321 ggcaacaccg ctatcgttaa aaacggcaaa gtcaccttaa tgcccaatga aaacgcaagc
     4381 ggtctgattt tgctggaaaa aagcaatacc gaaaatacgg caacgtttga ttggaaaaat
     4441 gccaccgtct attttgtgct gaccgaccgc ttttataacg gcgatccgac caatgaccac
     4501 agctaccacc gccaaaaaga cggaatgcaa gaaatcggca cctttcacgg tggtgattta
     4561 aaaggattaa ccgaaaagct cgattaccta caacaacttg gggtaaacgt attgtggatt
     4621 agctctccgt tagaacaaat gcacggctgg gttggcggcg gtgataaggg ggattttccg
     4681 cattatggct atcacggtta ctatcattta gattggacga aattagatgc caatatgggt
     4741 actgaagacg atttaagtcg ctttgttcaa caagcccacc agcgtggtat tcgggtgcta
     4801 tttgatgtgg tgatgaacca taccggctac gcgacgcttg ccgatatgca ggagtttaac
     4861 tttggcggat tttacttaac accggacgaa attagccgca cattaggcga aaaatggaca
     4921 cactggaagc cgaaatcagg acaaaattgg cacagtttca atgatttcat tcgctttaac
     4981 gataatcagg cttggcaaaa ttggtgggga aaagaatggg ttagagctga tattgccgat
     5041 tacgacagcc ctaaatttga tgatttaaaa atgtcgctcg ctgcccttcc tgatctaaaa
     5101 accgagagcg aacaagcggt caaattacct gaattttttg caaataaaaa taccaatgcc
     5161 aaaacattac caaacgccaa agtgcgtgat tatttaattg gttggttatc cgattgggtc
     5221 agaaaatatg gcattgacgg ttttcgggtt gataccgcca aacacgttga aaaatccacg
     5281 tggctggtat taaaacaatc ggcacaacaa gccttaaacg aatggcaaaa agccaatccg
     5341 aacgccggtt ttaacgatcg cttttggatg acaggagaag cgtggggaca cggcgtgttc
     5401 aaaagtgact attatcaaaa cggttttgat gcaatgatca atttcgattt tcaagatcag
     5461 gctaaacaag ggttaaattg ctttgcccat attgagccga tttatgccga aatgaatcgc
     5521 aaattaagcg acttcaatgt gttgagctat ttatcttcac acgatactcg attatttttc
     5581 cattcggata gtgaacaaaa tatcgaaaaa cacaaaaccg ccgcaaattt gctgttgctt
     5641 tcacccggtg cagtgcaaat ctattatggt gatgaaagtg ggcggacatt tggtgcgacc
     5701 ggttcagacc tgatacaagg cacccggtcg gatatgaatt ggcaagagtt gcagcaagat
     5761 cctcagaaac aggcattgca tcaacattgg caaaaattga cacaattccg ccaacgccat
     5821 ccggctattg gcgcaggtgt ccatcaactc atcaaaaatg atagctactt tgcatttaag
     5881 cgtgtgttga atgaagataa agtgatggtg gtttgggccg gaaattaaat ctactttagc
     5941 ttcaagtttt ctctcttctg gggaaaactt gaagccggtt cggggaaaca agttgcaaaa
     6001 aattactaaa aatagaccgc ttgttcccca tgagcttgtg atcaatgata ccccatcaac
     6061 atcagcagtt ctttcgtata gctgattgct tcgttacgat tcgtcacacc gattttctga
     6121 taaagatttc ggatatgggt tttaatcgtg gttgcagcca cttgcaattc atctgaaatt
     6181 tgttcattgc tatatccgga gtaaatcaaa cccaatactt gccattcccg ctgagtgagc
     6241 ggactaattt ttaagagttc cggcactttc ggatttttca ataattgatt cacaaagttt
     6301 tcatcaaaat gggcaaattt ttggcgataa aattgattga tgttgcgtaa aataaactgt
     6361 gctttgtgca ataccagttc atctagcaca ttaagttgca ataaatggcg gatctgctgt
     6421 gccattacat ccccttcaat cacaaacgca ctgataaaat tcgtttggcg ggtgagtttt
     6481 agtgcctgaa ttaaatcttg ctgagccaat tctttctcgc cttgtaaaaa ataaagccgg
     6541 ttacgaacaa ttaatgcgcg atttaaatcg ctaatcagag aaaatttttc agccgtttca
     6601 attaaattat ctaaaatggc tttcgcctcg tgatattcct caagcaagat tcgagaccgt
     6661 gcaatatttc gccattgcac ttgggtaaaa tgatttttat ctgaaaccgg tgaggggttt
     6721 tgaattaacc agttcctcac cgcagaaacg tcattggtca tctgccaata aaatatacgt
     6781 acctgatcgg cgttggcaat ccaatcgtgg tgataagaat gagaatgttc taattgttcg
     6841 acctcgccta ataatcgggc agtgttatcc aaattgcctc gagccaacga gatcttcgtg
     6901 agtaaagtta agcattgtaa cttatcttca aatttagata aggcattcat tccggcaact
     6961 gccattgatt cagctttatc taagttatac cattcccata aaatcttgcc tttagaacgt
     7021 agcaaaaatt catacatcgg cactttttgt agatggtttt ctttcacaaa ctcgcttgct
     7081 ttatctaaca tttcataggc tgcttgtgaa aacccttggg caagcaaaat ttcagattgt
     7141 tgaagctgtg accacaaaat attgtggtaa gtatgatgct gacgtgccat acgctctgct
     7201 ttttgcaaca ttgctaatgc ttctgccaga ttgccgtggc aatgatgagc ttcgccaata
     7261 atagacgttg caacaatatg agcataatag gcattttctg aaagatcgtt taacgcttca
     7321 gacgctaatt gaagtgcggt attctcatca ccggaattga tagcaacttg tgcccgtagc
     7381 acattaaatt ccgcattggc agttttgctc aattcaattt tattttcagt taatgcacga
     7441 gaaaaatccg ctaaaatacc acctacttca atatggcggt gctggctttg taccaaccag
     7501 gctttgagta acaccaaatt agtatgttcg gttaaatggg cgtaatctaa gcaatttaag
     7561 ctctcttcca ataatttgag ttctccttga tgaaacaccg tccaagcgtg tttgctcaaa
     7621 atattgagta gtaatccggt atccgataac tgcattgcgt gatgtaaggc ttctgtaaca
     7681 tagcctaatt gtaaccacgc attcgccgcc ttttgatgca gttgcggtaa ttcagcatat
     7741 aactcatttt ggcaacataa atttaaaaag gaagcaaaaa gcggatggaa tttccaccag
     7801 ctatcatcac tactctgcca cttactgttt gccatttgtt gtaaaaacaa accttgcttt
     7861 tcgagagact ctaatttggt gcgactgttt tgctcccctg taacagcctg cactaaagac
     7921 tcattcattg aatgcaaaac cgaacaacgc aaaataaaca aacgagtctc ttcatcgact
     7981 ttattaagca cttcatcggc taaatattcg ttaatatgaa aatggtttga tttggctaaa
     8041 cgtttggcaa tatcttgcag tggtacttgt agcgtgtgtg atttattttt tgcaaaaagg
     8101 ctaattagct gaagggctgt gggccaccct tcaacttcgt tacacagtgc gataacatct
     8161 tgctgattta actccgaacc taatcttgct tgaaaaaaag cggtactttc ttggtgatca
     8221 aacattaact ggttaatatc aatttccaat aattgttctt gcactcttaa actggcaata
     8281 cttaacgggg gaattgatcg agaaatcaag ataagcgtca tattagacgg ttgatgttta
     8341 atccaatatt ttaatgcttc gtgaatttca tcattttcaa taatatgata atcatcaata
     8401 gcaagataaa aatgctctga aaatgccgcg gctttaatca ataattgatt aaataaagct
     8461 aataaattag ctttacgatt ttcctctaac aatacgtcta tttcttcatt gattgctgaa
     8521 tgcaatgccg cactaaaata agtggcaaaa cgatctgaat tattatcgcc ttcatctaac
     8581 gcataccagc cgatattctt tttattttca atccattggg aaaccagagt ggttttgcca
     8641 taacctgccg gtgcatttat caatacgacc ggataactgc aagatttgtc taattcttga
     8701 attaaacgtt ttcgaggaat gctgttttgt aatcgaaaag aacaaacaag ttttgatggt
     8761 attaacataa taaattcaat tttctctcat aacaaaatag ggatgatata tccaccattt
     8821 gtgttctcac tacttcctta taatcatacc atacaagatg attattggga tgatttgatt
     8881 ttatttggat tttacataat agcgacattc atctttaacc gggggtttcc tatgacattt
     8941 aattctattg tagaaaaata ctgtcgctac tttgatgtga cagatccaaa acactttacc
     9001 ttacagcagt ggtatcaagt tgttgctgaa ggttcccttg agctaattta tagccagcct
     9061 gcaaccaaac cgacagaatc acgccacgtg aactacctct caatggagtt cttaatcggt
     9121 cggttaaccg gcaataattt aatgaattta ggttattacg aacaaattcg tgactatctt
     9181 aaacaatatc aagttgaatt agtagatgtt ttagaacaag agcgtgatcc ggcattgggt
     9241 aatggcggat taggtcgtct tgctgcctgc tttttagatt cgatggcaac tgtcggacaa
     9301 aatgcaaccg gctatgggct tcattatcaa tatggtctgt ttaaacaaag cttcaaagag
     9361 ggaatgcaga aagaagcagc ggatacttgg gctcgtgaca gctacccgtg gcatcgtttt
     9421 aatccgtcta aaacccgcca tatcgggttt ggtggaaaaa tcaaacatat ccaagccgat
     9481 caatatgaat ggcagcctaa attgaccatt caaggcaaag cctttgactt gccgattgta
     9541 ggttataaaa acaacatcat tcaaccatta cgtttatggc aagcagatag tgaccaatct
     9601 tttgattttg atgcctttaa tgagggtaaa tttttaagtg ccgataaaac gattgtcaat
     9661 gcagccgcac taactcaagt gctgtatcca aacgacaacc atcaagccgg acaaaaatta
     9721 cgcttaatgc agcaatactt ccattgtgcc tgctcggttg ccgatatttt agatcgtcac
     9781 attagcgaag gttattcgtt agaagattta gcaaaacgcc aagttattca actcaatgat
     9841 acccatccaa cgctcgctat acctgaattg atgcgtgtac tgttagatga acatcaattc
     9901 acttgggagc aggcttggga tatttgcagc aatacctttg cttacactaa ccatacctta
     9961 ttacctgaag cacttgaaca atgggatcaa cgcctattta agcagctatt acctcgccat
    10021 tatcaaattg tggaaaaaat caatgatatt ttccacgaca aagtacaggc agaatttggt
    10081 gatgatgcaa aaatttggga aaaattagcc gtgctatttg attaccgtgt gcgtatggca
    10141 aacctctgcg ttgtcacctg cttccgtgta aacggcgttg cggaaattca ctccgatctc
    10201 ttagtgaccg atctattccc ggaataccac aaattattcc caacaaaatt ctgcaatgtt
    10261 actaacggca tcacacctcg ccgctggatt cgacaagcta atccacaatt aagtgattta
    10321 ttggatcgta ccttaaaaca agattggaca cacaatctgg aattattaaa aggcgttgaa
    10381 caatatgcgg atgacaccgg tttccgtgaa gaatatcaac gcattaaacg caataacaaa
    10441 gtggttttag ccaatgaaat ccataaaaca ttagggttaa atgtcaatcc taatgcgatt
    10501 tttgatgtgc aaattaagcg tttccacgaa tataaacgcc aacatttgaa tttgttaaac
    10561 attattgcga cctatcaatc gctaaaagct aacccgaacc aagactacac accacgtcta
    10621 ttcctattct cgggcaaagc tgcaccgggt tattatttag caaaaaatat tattcacgct
    10681 attaataatg tggcagatat tattaataac gacaagcagg taaatgatcg actacaagtg
    10741 gcattcttac cggattatcg agtcagtctt gcggaaaaaa ttattccagc agctgatgta
    10801 tcagaacaga tttcaatggc aggtaaagaa gcctccggta ccggtaatat gaaattggca
    10861 ctcaacggcg cattaacctt aggtacgcta gacggtgcca atgttgaaat tgctgatatg
    10921 gtcggtgagg agaatgtctt tatcttcggt cacaccgtag aaagcgtgag ggaattatta
    10981 gcaaaaggct accaaccgaa agcctactat aaccaagatc cgattctgaa aaatgcggtt
    11041 gatttcttag ccacaggaaa agcctctaac ggagataaag aggcattcaa gctgatgtta
    11101 gatagcctgt tggaacgaga tcctttctta gtctttgccg actttgatag ctatcgccaa
    11161 gcacaagaga aaattggtgt agcctattta gatcaagaaa cttggctgcg tagtgcaatt
    11221 ttaaatactg ctcgtttggg tgcatttagc tcagaccgct caattaaaga ttatcaacaa
    11281 cgtatttggt tgaatagata ataaattcgg gcgacttcta caataaagga agtcgcctat
    11341 tttctgctgt atttcatatg tatttatata tggagagaaa tgatgaaaat aactgccaaa
    11401 caatattatc gggctggaat tatgccctat tttttcgatg agcgtggtgt aaaaaaaaca
    11461 gcatctgaaa aaataaaaca ttcgttattc aaagccttta acggcaatac tcaagcaaga
    11521 gaaacaccta tccctacggt caacattttt taccagcacc aaccctgttt tttacctttt
    11581 catttagcgg ataaaaaaca tccgttaacc ggtacttggc agctacaact agaaaattca
    11641 aaccaaatta tttccggcaa aattaaagca aggggaatta cattacctca agatttgccc
    11701 ttaggttatc accaattaca gctaaaaacg gtagataaaa cctttaattg caccattatt
    11761 atcgctccgg aacgctgcta ccaaccacaa gccgtcattg aacaaaaaag attgtgggga
    11821 accttcctac aactatacac cttaaaatca gcccacaact gggggattgg cgattttggc
    11881 gatttaaaag acttcattca acagattgcc ccttaccaag cagattttat cggcttgaac
    11941 ccgattcact cactttttcc ggcaaacccg aattctgcca gcccatacag tccatcctct
    12001 cgccgatggc tgaatattgc ttacattgat atcaatcagc tagcggaatt tcaacaaagt
    12061 gatgaagcac aaacttggtt tcattctgct gaaatccaac aacaattatc agaattacgc
    12121 actcaggatt ggttgaatta cgagcaaatc attccgttga aattgaaagg gctacgcttt
    12181 gctttcgcaa aatttcagca aaatatgacc gcttgcgctg cccctgcttt tatcgaattt
    12241 gtacaaaaag gtggtgaaag cttacaagtt caagccacat ttgatgccct tcatcagcat
    12301 ctatcggcac aatttgccga ccaatggggc tgggatttct gggagaaagc ctaccaagac
    12361 taccactccg cagccgtgca gcaatttaga gaaacgcata aaaccgacat agaattttac
    12421 ctttggttac aattcgttgc cgatcaacag ctagcagaat gtgatgctat ctgccgtcag
    12481 caaaatatgc gtattggtat gtatcgcgat ctcgccgttg gcgtcagccc gaatggtgca
    12541 gaaacgtgga atgacaaaca actttattgc ttaaatgcct ctgtcggcgc tccgcctgat
    12601 gtattaggtc cgcaaggaca aaactggggc ttaaccccaa tgaatccgca tgtattaaag
    12661 caacaagcct accgtccgtt tattgagtta gtcagagcta atatgagtca ttgcggtgca
    12721 ttacgccttg accatattat gtctttactc cgtttatggt ggatccccaa aggcgatagt
    12781 gcggttaatg gtgcttatat tcgttatcca gttgatgatt taatcgcaat tttggcactt
    12841 gaaagccaac gccaccaatg cctaattatc ggtgaagatt taggtactgt gccaaaagaa
    12901 attgtgagca agctcaaaaa tgcggggatt ttatcttaca aaattttcta ttttgaattt
    12961 gatgaacaag ggaagagtcg agacttacag cattaccctt accaagcaat gaccacatta
    13021 agcacccacg atttaccgac tatcaatggt tattggcgtg gctatgattt tgaattaggc
    13081 gaaaaattcg gcgtctatcc taattcgaat attttggcaa tccttaagca agatcgagtc
    13141 aatgccaaag ccaaaatctt acaacgctta caagaacata acgtagagat cgcacctgaa
    13201 attagcgagc aacttaattc tccggtgaat accgcattta ttcactgttt acaaacctat
    13261 gttgcaaatg taagcagcgg attattcggc ttccaacccg aagactggtt gggtatgact
    13321 gagccggtta acattccggg aaccagtaca caatatgcaa actggcgacg tcggcttagt
    13381 gttaacctcg aggagatctt cacagataaa gatattcaag ccttattaaa agaagttaat
    13441 ttaatccgta aaaatcaata aaagttagat aagaaaaaag gcgactttcc gtcgcctttt
    13501 gggcttatat gcttgatgtc tatctttatc cacctgaaac ccacaacata atcgctagca
    13561 cttgaggtag cataatacgc aaaaacatta ctagtgggta aacagttgca taggataacg
    13621 ctgcagcgcc atttccctct ttaatttcat tggcaaaagc aagcgctgga ggatcagtca
    13681 ttgagccggc aagtaatcca caaatcgtta aatagtttac tttgttatac attctggcaa
    13741 tcaatcctgc tatcatcaat ggaacaaaag taataatcgc cccatagcat atccactcaa
    13801 cgccctcact gcttaatagc gtttcgagaa atctaccgcc agattttaaa ccaactaccg
    13861 ctaaaaacag tacaataccg atttcacgca atgcaagatt ggcacttggt ggcataaacc
    13921 agtagagttt actgaaacta ccaattcttg caagaataag agcaaccacc agtggtccac
    13981 cagccaagcc cagctttaac gcaacaggga aacccggtaa ataaatcggt acagaaccaa
    14041 gtaatactcc caaaccgatc ccgataaata ttggcaacat ttggacttgt tgcaactttg
    14101 agtgcgcatt gccgataatc gtcattacca tttcaatatc agcttgccgc cctactaaat
    14161 tgagaacatc accaaattgt aacgtcatat cacctgttgg tactaattct acaccggctc
    14221 gatttaaacg agaaatgaca acatcgtatt tgcctttaag cattagctga cgaatttgtt
    14281 tcccgaagac tttttcattc gtcactacag cacgttcaga acggtaaatc gtgcctttgg
    14341 ttgagacaga tacatctact gcctcaccta aaataatctg catttttcgt aatgaggatt
    14401 cttcacctac taagtgtaaa atatctccta tctgtagaat cgtatctaca cgtggaatag
    14461 aaagctcatt cccccgtttt aagcgtgaat aagctacttc gtgcaaatta aaatcaggaa
    14521 tttgtttcaa cgttaaaccg tgaatatttg gattagtcag acggacattt aaggtactaa
    14581 gttgatcttt acttctattt tgctgtttgt taaaagcctc tgcttccgcc tcaatattga
    14641 ttcgaaataa aatccgaatc agccacattg aaagtagaat tccgataatg ccgaacgggt
    14701 aagcaatcgc ataactcatt cccataattt cagttacact ctcagcgctt aactcagcca
    14761 atacctgttg acctgctcct aaagaagggg tattggttac agccccggag aaaatgccta
    14821 aaatcacagg caaaggaaca ttgaagaatt tatgaataag aataactaaa ataccgctaa
    14881 tcagtacaat cagtaccgca aaaccattga gttttaagcc agaatgtttt agtgaggcaa
    14941 agaatccagg tcctacttga attccaattg aataaacaaa taaaatcagc ccgaactctt
    15001 gaataaaatg aagcgtatgg gcatcgagct tcacatcgaa ttgcgccata aaatgggaga
    15061 caaacagccc accaaataac actcccccta tacctagccc cacgcctctg attttaatat
    15121 ggccaatcca taagcctaga acggccacta aagataataa actgacagta agtgcaattt
    15181 cgctcattac agcctctctt taatatgaaa tacctcaaaa atggtatcca attctaccaa
    15241 atctcaaaaa cgaaaaatga gatctatgtc aaaaaaataa aatattgata gtattactcc
    15301 aagatgaact tcaaaaaagt tcctgaattt aaaaaaaatt ctcgacaata ctttttttta
    15361 cagtaaatta aacaattatt taaataacct aaggatcaaa cacaatgtta tcaaacgaag
    15421 tctttatttc gattatcgtg ctgttaatcc taagcttgct tagaattaat gtagtaatcg
    15481 cacttattat ttctgccctt acttgcggtc taatcggtaa tcttggtgta gaaggggctg
    15541 gatttaccga agcattggaa aaaacaataa aaagttttac tggtggttta ggtgggggtg
    15601 cggaaactgc tatgaactat gccgttcttg gtgcgtttgc cgtagctcta tcaaaatcag
    15661 gcgtgaccga tttattagcc tataaagtga tcaaatcctt tggtcacagc ccgtcaggtc
    15721 gctccgttac ttggtttaaa tacattgtat tatttgtgtt agtggcattt gcaatgtcat
    15781 ctcaaaacct aattccaatt catattgcct ttattccgat tttaattcca ccgctattaa
    15841 gcgtaatgaa taaattgcaa attgaccgcc gtgcggtagc ttgtgcttta acctttggtt
    15901 taaccgccac ttatatgtta attccggcag gtttcggtca gatctttatt gaaagcatct
    15961 tagttaaaaa tatcaaccaa gcaggtgaac cgttccaatt agtgactacc acagcggaaa
    16021 tgaccaaagc gatgttaatt cctgtttccg gtatgatttt aggcttactg tttgcattct
    16081 ttgtgtcata ccgtaaaccg cgtgtgtata ttgaaaatat gcctgaacca accgcagatg
    16141 atattgaagc ccgtgttgca aacgttaagc ccttccatat cggcattagt attgtggcga
    16201 ttatcgcgac ctgttcggtg caattagcaa caaattcgac tattatcggc ggcttagtcg
    16261 gtctggttat tttcgcctta ggcggtgtat ttagactgaa agagagtaac gatgtcttcc
    16321 aagctggctt acgtttaatg gcgatgattg gctttgtgat gattgccgcc tccggttttg
    16381 ccggcgtggt caattcaaca ggcggtgtgc cggcattggt agaaagcctg cgtgatgtga
    16441 tttcatcaaa agaaatggca gcattgctaa tgttagtcgt tggcttattt attacgatgg
    16501 gtatcggctc ttcattctcg actgtgccga tcattacctc aatttatgtg ccgctttgta
    16561 tctcatttgg cttctcgcca ctggcgacaa tggcgattgt cggtgttgct gctgcactcg
    16621 gtgatgcagg ttctccggca tcagattcca cacttggtcc aacctccggc ttgaatgctg
    16681 acggtaaaca cgaccacatt tgggattctg ttgtaccaac ttttatccac tttaacattc
    16741 cattattagt gttcggctgg attgcagcaa tgacacttta agctataata tgcaaaattt
    16801 ttgctaaaaa tgaccgcttg tagccattac aagcggtcta ttttcttctt tattttgcaa
    16861 accacattag gatcaccaat gaaatttatc tcttttaata tcaacggatt acgagccaga
    16921 ccacatcagc tcgaagcgat tatcgaaaaa caccagcccg atgtactcgg cttacaagaa
    16981 atcaaagttg ccgatgaaga ttttccttac gatttagtca atcacttagg ctaccacgtt
    17041 ttccaccacg ggcagaaagg tcattacggc gtggcattac tgactaaaaa agagcctata
    17101 gcggtgcgta aaggcttccc gacagatgta cttgatgctc aaaaacgaat gattatggcg
    17161 gatatcgaga ccgatttcgg cgtgctaacc gtattaaacg gctatttccc acagggggaa
    17221 aatcgcagcc acgaaaccaa attcccggca aaacaaaaat tctatgctga tttgcagaac
    17281 tacttggaaa gcgaattaaa gccggaaaat ccgattatta ttatgggcga tatgaacatc
    17341 agcccgactg atttggatat cggcatcggc gagccaaacc gcaaacgctg gctgcgtgac
    17401 ggtaaatgtt cattcttacc cgaagagcgt gaatggctag atcgcttata cagctacggc
    17461 ttggttgata ctttccgtgc aggcaaccca gacgtgaacg ataagttctc ttggtttgac
    17521 taccgttcaa aaggttttga tgataatcgt ggcttgcgta ttgatttaat tcttgcttca
    17581 aaatcacttg ctgagcgttg cgtggaaaca ggcattgatt tagacattcg agcaatggaa
    17641 aaaccgtctg accactcccc tgtttgggca acctttaaat aaagttgcaa aaaaatctga
    17701 gttttcaacc gcttgtcagt ttttagccct acaagcggtt attttttctc acttttttgc
    17761 actttttacg ctgtactata tccctattca aatctgtgct acaatcaaac actattttta
    17821 gattattttt ggattatgaa caaacgttta accgttattt tactgattat tgttgccgta
    17881 cttggtggct ggtattacag cttacaagag aaagagaata cggggcttga tcagcttatt
    17941 aaaaaggaag gacagcctga atatgtgggg aataaaatgt ccaccacggt ctatgatcta
    18001 aaaggaaacc cacaatattt cgctcaagct actgaaatta aacgctatga gtctactgag
    18061 cgtactgagt tttttaaacc ttttattgag ctttttgcca aagagtcgac gcaaaaacag
    18121 tggaaagtca gtgccgatta tgcagaaatt accaaagaaa aaatgttaaa tttaaaaggt
    18181 aatatcaaac ttgttgcatt agatcctgca tcacgtttgc aacaaatcac taccgatacg
    18241 ttaactgttg atttaaatac acaggatatt tttaccgaaa gtacggtcaa atcgataggt
    18301 tccggtttta ctacaacggg ggttggatta aaaggtaact taaaacaaca agttgcgact
    18361 ttgcaaaaag atgtaaaatc ttacattgaa ccgaccatta ttcaagaaac taaagaataa
    18421 tttgcttgga acaaggaata acaatgaaat taatgtttaa atctatttta gtgagcgtgt
    18481 tactgggtag cagcttttcc gcttatgctc taaaaggcga taccgatcag ccgattaata
    18541 ttgattcagg tagccaatcg ttagatatga ctaacaatat cgtgaccttt agcgataatg
    18601 tcgttatcac tcaagggtcg attaaagtga ccgcagcaaa cgtcaaaatt acccgtcaag
    18661 aaggtaaaaa agaaactatt gacgctacag gctcacctgt aacattccaa caaaccttag
    18721 ataacggtaa accggtaaac ggtaaaggca acagcgttca ttatgattta aactctgaat
    18781 ttttaacgct gattggcaat gcggaactca aacaacaagg tagctttatt aaagccaata
    18841 aaattaccta tgatgtgaaa aaacaacaac ttaaagctac cagtggcggt aaaacccgag
    18901 tgaaaaccgt gctaattcca aatgaacttc aagataacaa gaaaaaataa ggttaaatga
    18961 tatgtctgtg ctttatgccg aacatttagc aaaaagctat aaacaacgcc aagtggtaaa
    19021 agacgtcagc ctgaatgtca aatcaggtga aatcgtgggc ttgctcggtc cgaacggcgc
    19081 aggtaaaacc accactttct atatggttgt gggtttggtt cgccacgacc acggcacaat
    19141 tcgtattgac gaccaagata tcagcacact accaatgcac gacagagcaa aacaagggat
    19201 tggctatcta ccgcaagaag catctatttt ccgccgttta agtgtgtacg ataacttaat
    19261 gtcggtatta caagtgcgta aaaatatcaa tgataaacag cgtaaagagc gtgccgaaga
    19321 gctgattaca gaattccata ttgagcatat tcgcaatagt ttaggtcaat cactttcagg
    19381 tggtgaacgc cgccgtgtgg aaattgcccg agcattagca gcgaacccga aatttattct
    19441 gttagatgaa ccttttgctg gggttgaccc aatctcggta attgatatta aaaaaattat
    19501 cgtcaattta aaagagcgtg gtttaggggt attgattacc gaccataacg tacgtgaaac
    19561 tctagatgta tgtgaaagag cctacattgt cggtagcggc gaaatgattg ccagcggcac
    19621 accacagcaa gtgcttgaaa accctgatgt aaaacgtgtt tatttaggcg atcaattcaa
    19681 actctaaaaa atcccctctt tggggatttc atctttagat gaaaaattat gacaaaacta
    19741 accgaatttc taagccctga aaatgttcgc caaggtgtac tgatttcaag taaaaaacga
    19801 gcgttagaac tcgtgggtaa aatcgtggct gaatcaatca accaaaccga acagcaatct
    19861 gaagcactct gccctattga atgctttggc aatttattta aacgtgaaaa gttaggcaca
    19921 accggcttaa atcaaggcat tgcacttcct catgcaaaat tgcccgcttg cggtgatatt
    19981 gagctgacta aaccgattgc ggtcttttta caactggaag aagcgattga ctacgaagcc
    20041 caagacaaca aagaagtgga tttaatttat gcaatgatgt ttccggaaaa tagctgcgaa
    20101 cagtataaaa gttgcctacc tgcaattgca cagaaactta ccgataaatc gttattaaaa
    20161 cagctccgag cagctgaatc cgcagaagat atttggcaaa ttttggtgta tgctgataat
    20221 cacctaaaag atgaagaata atactgagga aatatggaat taatcattat aagcggacgc
    20281 tcaggctctg gtaaatcggt agcattaaga gcattagaag atgcaggtta ttactgcgtg
    20341 gataatatcc ctttaccttt aattccaacc ttaacctact accttactca agaaaatcgt
    20401 tcggcagtca tcagcttaga tattcgtaat ttaccgaata cgcctaatgc gattgaagaa
    20461 ttgcttaccc agcttgagcc tttaaatgct aagctgattt ttttagattg cgaacgcaat
    20521 accttaatcc gccgttatag cgattcccgc cgaatccacc cgctttccac ccaagacgat
    20581 ctctcgttag aaggtgcatt ggatttggaa caaaaattgc ttgagccttt ggtgcaaaat
    20641 gcaaattata ttattgacac cagtcatctt tcttcgcacg aattagcgga aaatcttcgc
    20701 caaatactgc aaggctcaac cgaaaaagag cttaaaatta ttttccaatc attcggtttt
    20761 aaatacggct tacctgccga ttccgattac gtttttgatg ttcgcttttt accgaaccca
    20821 cattggaatc cggagcttcg cccaatgaca ggcttagagc aaccggtgat tgatttctta
    20881 gaacgccaaa ccgaagtgca taattttatt taccaaaccc gcaattattt agaaatgtgg
    20941 ctgccgatgt tggaacaaaa taaccgtagc tacctaacta ttgcgattgg ttgcactggt
    21001 ggaaaacatc gttccatatt tattgccgag caactggcaa aatatttcca agcaaaaggc
    21061 aaaaatgtgc aagttcggca taaaagtttg gaaaaacacc ataaaaaaac atcttaaata
    21121 agtaggggct ggtcctgtgc cagccctcat ttcaactatt ttagggcggg cacagggcac
    21181 cgcccctaca tttgatttta tgaaatccgc agatattgtt attgttggcg gcggtatggt
    21241 tgggctggct cttgccggac tgctcaaaga tgtcgattgc caaattaaaa ttattgagaa
    21301 aaacacgccc aaatttgatg ccgatacgat ttaccaccgt gtaagtgcga ttaatgcttc
    21361 aagccaaaaa atactggaac agattggtgc ttttcaacac attccggctc aacgcctttc
    21421 tccttacacc gaaatgaaag tgtgggaaaa agacagcttt gctaatatcc attttgataa
    21481 taatgataat gaaatcaagc aattaggact ttcacaactt ggctttattt tggaaaatgc
    21541 ggtgattcaa cacgcattgt ggcaacaggt ttctcaacaa gctaatgtag aatatattat
    21601 cgccgccccc caaactctgg gcgtgagcga aaacggggca tttttaacct tagataacgg
    21661 cgaaatgctg tctgctaaat tggttgtcgg ggcagacggt gcaaattcgt gggtgcgtaa
    21721 ccagatgaaa atgccgctta ccagtaaaga ttatcaacat accgccttag tctgtaatgt
    21781 caaaacaacc gaaaagcacg gcaaaaccgc tcgtcaaatc tttgctgaag atagcatttt
    21841 tgcttttctg ccaatggcag atgagcatct ctgctcgatt gtttggtcgc tgccaccaaa
    21901 caaagccaat gaattaaaaa atgcaagcga agccgaattt aataaagcct taacgattgc
    21961 cttcgataat cagcttggct tatgtgaagt gcaaagtttg cgtgaaattt acccgcttgc
    22021 agcacgcttc tgccgtgact ttgcccaacc tcgtattgcg ttgattggtg atgccgctca
    22081 taccattcac ccattggcgg gacttggtgt caatttaggc tttgccgatg ccagaacgct
    22141 ggcaaacgaa atccaaaaac agttggtaaa caaccaagat attggtgaat accgccattt
    22201 acgccaattt gaacgtgagc gtaaagccca agcgatgaaa ttattagcca caatggaagg
    22261 attaaaacag ctatttgacg gcaatcaccc gcttaaaaaa ttattcagag gaattggttt
    22321 aagcctaacc aaccagctgc cttttgtgaa aaagcagctt attcagcaag ctatttactt
    22381 ataaaatgta aattttttcg aaataacgta aagatgaacg attttatgaa ctaatacgga
    22441 gattacttgt ctatttattg gtacaataac cagcctttat tggcttttat ttttatattc
    22501 ttgaggaatt ttttaatgaa caaattaaca aaaatcagtg caaccgcttt agtcgcatta
    22561 ttcttagcag cttgtgataa accagcagaa aaagcaccgg caacagaacc tacaaaaaca
    22621 actgaagctg caccaactac aacagctcct gctgcagaag cagccaaacc tgctgaaaca
    22681 gctgtagccg ctccaagcga agctgcaaaa gcagattacc aaaaattagt tgattggaat
    22741 acggcacaag aaagcacaat ggcaacagca caagcagaac tacaaaagaa attagctaca
    22801 caagatccta aacaaattca agaaggatta agtgcattta ataaaaaagt agaagaaact
    22861 gtaaaaagct tagatgcgat tgaagtatct gatgcacaaa tcaaaacatt taaagaaaaa
    22921 acaaaatcag ttttaacact ttcaagtgaa gtgatttctg aacaagtaaa aacgatctct
    22981 acaccaaatg accaagcagc attacaagcc gtacaacaaa aagctcagtc tttaattgaa
    23041 gctggtaatg agttacaaaa attaaacgta gaattacagc aaagattctc agctaaataa
    23101 tttaccattc aatgaaaagc tagagtattt tgctctagct tttttttgca attactaagg
    23161 attactatga acacgctcgc taaaattagc ctatctgtat tatttaccct ttttttaacc
    23221 gcttgcgaac aacctaattc taccaaaaca cagagctcag cagaatcgcc agttcaagtg
    23281 aaagaagagt caaaagaaga agttaaacct gctgataccg gcgctcaaga ttataaaatg
    23341 ttacgggaat ggcaagacac tcaagaaaaa gccttaaatg atgcaattaa agcggcaaca
    23401 gaaacactta ctgacaaaca aaaagccgat tctaccttaa tgcaagagac agttaataac
    23461 gcattattag ctcaaattga tcatattaag ataagtgctg aaaccctgaa tattcaaaat
    23521 aatgaagtca aagcactaaa agacaaaacc ttagaagtcc tgactttagg ggctcagatg
    23581 atcgtagaag gtgctaaaat ggagaaaaat cctacaccgg aagcacataa agcatttggc
    23641 gagttacaaa caaaattgaa tcaattagct gaagaagggc aacagttaga aaatatatta
    23701 agagctaaat atg
//
LOCUS       DANLXF010000006        23464 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.006, whole genome shotgun sequence.
ACCESSION   DANLXF010000006 DANLXF010000000
VERSION     DANLXF010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 23464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23464
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.006"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..1604
                     /gene="pckA"
                     /locus_tag="PXD66_000145"
     CDS             <1..1604
                     /gene="pckA"
                     /locus_tag="PXD66_000145"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDL6318486.1"
                     /translation="NRIEQELADLGITNVKEIVRNPSYEQLFEEEMKSDLKGYEKGVL
                     TNLGAVAVDTGIFTGRSPKDKYIVLDETTKDTVWWTSDAAKNDNKPMTQSTWQSLKTL
                     VTDELSNKRLFIIDAFCGASPEHRINVRIITQVAWQAHFVKNMFIRPTEEELKDFKPD
                     FVVMNGSQVTNPNWKEQGLNSENFVAFNITEGVQIIGGTWYGGEMKKGMFSMMNYFLP
                     LKGVASMHCSANVGEEGDVAVFFGLSGTGKTTLSTDPKRKLIGDDEHGWDENGVFNYE
                     GGCYAKTINLSIENEPDIYRAIRRDALLENVVVREDGTIDFDDKSKTENTRVSYPIYH
                     IDNIAVPSKAGHAKKVIFLTADAFGVLPPVSKLTPEQTKYYFLSGFTAKLAGTERGIT
                     EPTPTFSACFGAAFLSLHPTQYAEVLVKRMEAAGAEAYLVNTGWNGTGKRISIKDTRG
                     IIDAILDGSIEKAEMSTLPIFNLAIPTALPGVETHILDPRNTYEDKAQWQAKAEDLAG
                     RFVKNFEKYATNEEGKALIAAGPKL"
     gene            1697..2698
                     /gene="bioB"
                     /locus_tag="PXD66_000146"
     CDS             1697..2698
                     /gene="bioB"
                     /locus_tag="PXD66_000146"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDL6318487.1"
                     /translation="MTTFQLRLKAKDELTPHPTAQYWRKCQVEGLFDLPFMDLVFRAA
                     QVHRENFDANAIQLSTLLSIKTGGCPEDCEYCPQSARYDTGIEKQTMLDVETIVEKAK
                     IAKERGASRFCMGAAWRGPRPKDIKVVSEIINAVKSLGLETCGTFGMLEEGMAEELKQ
                     AGLDYYNHNLDTDPERYNKIIHTRNHEDRMDTLGKVRNAGLKVCCGGIVGMNETRPER
                     AGLIASLANLDPQPESVPINQLIKVEGTPLTNAEDLDWTEFVRTIAVARITMPQSFVR
                     LSAGRTTMPEAMQALCFMAGANSIFYGDKLLTTENPEEDHDRQLMDKLDLYPLQYIA"
     gene            2858..2955
                     /gene="ffs"
                     /locus_tag="PXD66_000147"
     ncRNA           2858..2955
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PXD66_000147"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            2990..3697
                     /gene="gloB"
                     /locus_tag="PXD66_000148"
     CDS             2990..3697
                     /gene="gloB"
                     /locus_tag="PXD66_000148"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HDL6318488.1"
                     /translation="MLQITPIPALKDNYIWAIRNGQDVIIIDPSEHQPALNFIAKNAL
                     NLTAILITHNHHDHTDGVSGIVEQYPNIPVYGPQEVAEFANVIVSPEQHISLFGYDVR
                     IIESGGHTAGHISYLFGYDYLFCGDALFSGGCGRVFTGDYQAQFDALQRFKNLPNSVK
                     AYPAHEYTKSNLKFAEAVLPPSCVLSEYQEQVDLLRSQNKPTLPTTIGLEMQVNPFMQ
                     AVDLAEFIALRQQKDNF"
     gene            3856..4569
                     /gene="uppS"
                     /locus_tag="PXD66_000149"
     CDS             3856..4569
                     /gene="uppS"
                     /locus_tag="PXD66_000149"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005619022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HDL6318489.1"
                     /translation="MNFDPQNMPKHVAIIMDGNGRWAKQKGKLRIFGHQNGVKAVRSA
                     VSFAAKHHIQVLTLYAFSSENWSRPETEVSALMSLFMKALDSEVKQLHKNNIRLKIIG
                     DKSAFSENLQKRIAESEKLTENNTGLILNIAANYGGYWDIVQAAQQLAVHVKSEQLAV
                     EQITPALFQQHLVTENQPQVDLLIRTSGEQRISNFLLWQIAYAELFFSPVLWPDFNDE
                     AFSDAIYAYQQRERRFGGC"
     gene            4593..5462
                     /locus_tag="PXD66_000150"
     CDS             4593..5462
                     /locus_tag="PXD66_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDL6318490.1"
                     /translation="MLKERVLSAIIMIIAVLAAIFWLSPLPLTLVLSAIVVAAMWEWA
                     QFAGFKHPVPRAIVAMVTICLLLFAIVANTDYIRATRFITDETTPILFIGCIWWLIAL
                     VLVATYPNSAKLWEKSVAAKFLFGLATLVPFLIGTLALRFHNYAFDPYQGTYLFLYVC
                     LLVWGADSGAYFAGRAFGKRKLAPKVSPGKSWEGVIGGVLTSSLIAFTFLQLTPANVF
                     GKALATTPFILVSVGMVAISVLGDLAESMFKRQAGIKDSSQLIPGHGGILDRIDSLTA
                     AVPFFATFFFFVL"
     gene            5466..6776
                     /gene="rseP"
                     /locus_tag="PXD66_000151"
     CDS             5466..6776
                     /gene="rseP"
                     /locus_tag="PXD66_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDL6318491.1"
                     /translation="MTSIIAFFILICVLVFVHEYGHFWAARKCGVKVIRFSIGFGKVL
                     FKKKDKQGTEFVFSLIPLGGYVQMWNDETDINAPAQQALNTKSVLQRAFIIIAGPAAN
                     FIFAILAYWVVFIAGIPTVKPVIGEILPNTIASQARIPTELEITKIGHQSIQDWESAS
                     LALVGFVGNKNVPLEGNINGNSTQKYELDLSNWSIDSAKENPLTALGIRPKSAKVTAE
                     IKQVVENSPASQVGIQAGDRILQINQQPFNWFNLVELVQAGKPIELKIEQRGQIKDLV
                     VQPEKKDERYIIGVIPSYEPLADKYRTELKYDILTAFYKSIEKVWSLTQTILQFIGNL
                     ISGDLSIKNLGGPISMAKGAGATAEIGLVYYLSFMALISVNLGVMNLFPLLPLDGGQL
                     VLLAVEAIRGKALSEKIQLKFQQIGFAFVLSLMLFAFANDIIHF"
     gene            6891..9272
                     /gene="bamA"
                     /locus_tag="PXD66_000152"
     CDS             6891..9272
                     /gene="bamA"
                     /locus_tag="PXD66_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HDL6318492.1"
                     /translation="MKKLLLSSLLIANGVVAAPFVVKDIRVEGVQPTTGESIISSIPV
                     RVGQTATDTDVSNVVRHLFSQQRFADVRATREGNTLVIKVAEKPIIGKVEIEGNQAIP
                     KDALEQNLKANLINQGEIFDAAKLEAFKEGLIEHYHSTGRYEAKIDTSVSNNTEGGVN
                     IKLAINEGEVAKAKTIKFEGNNAFSDKKLLENLDIQPDVSWWNIFESSKFEQTAYQKD
                     LETLRDFYLNRGYAKFAIQDTDVQFNDNKTEVALTYKLDEGSQYHVSEMRIIGNTANM
                     DSQLNALLKEFKPGQLFRKSDLTQIEEEIKSILGDAGYGSAKVDIYPQFNEENKTVKI
                     SFVVDAGRRIYVRKIRFEGNGVTADSTLRREMRQQEGAWLSTNKASLGKARLERTGFY
                     ESVDMSMPNVEGTADQVDVVYKIKERNTGSINFGIGYGTENGISYQAGIKQDNFLGMG
                     STISLNGTRNNYGTSVNLGYTEPYFTKDGVSLGGNIFYEDYDNSDNDSMASYKRQTYG
                     INGTLGFPVNENNSYYLGLGYTHDKIKNAAREYTREKYVKSMNFEFDPAADYYKKIKA
                     DDFDFSLGWNYNSLNRGYFPTEGTTANIGGKITIPGSDNKYYRVSADFRNYYPLNREH
                     KWVISTKASIAYTNGFGGKEVPFYQLYTAGGIGTLRGFSYGAVGPQAIYYNTKSGKFD
                     SMNNDIIGGNALAAASLELITPTPFVSEKYQHNVRTSLFVDAASAWNTKWKKDEYTNL
                     PDYGDFKRFRASAGIAFQWMSPIGPLSFSYAKPIRKYDNDDIEQFQFNIGSSF"
     gene            9352..10143
                     /locus_tag="PXD66_000153"
     CDS             9352..10143
                     /locus_tag="PXD66_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HDL6318493.1"
                     /translation="MKKLFKMTGLAVALASTTGIANAADTIGFVDPSYVLQNHPVLLD
                     ASAKFDKFMKESQEKFAADEKKLADENKTLTAERNKINEDAQKLQNEQKNVEASIKKK
                     VAALEKDAPRLRSKEIQARQNAIQKEANAFQAKVTAIQKREADFAKKAEAFQKRADEF
                     QKRIEQANKENGGINPQEAQKQAVDEVNATIKEIAKSKGYTLVLQPQVALYAEDEGKD
                     ITEAVLDAIVKKHPEIKIEKPATEAQPATEKETKAEETKPEEAKK"
     gene            10143..11168
                     /gene="lpxD"
                     /locus_tag="PXD66_000154"
     CDS             10143..11168
                     /gene="lpxD"
                     /locus_tag="PXD66_000154"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HDL6318494.1"
                     /translation="MANFRLIDLAEHIGGTLRGNADLAIQSIAALDKADSSQITFISN
                     AKYRENLTACNAGAIIVSPADVEFCRADQNLIIVANPYVAYAQLAQYMDSTPKAASEI
                     HPSAVISPEAKLGNNVSVGANAVIESGVELGDDAVIGAGCFVGKNSKIGARTQLWANV
                     SVYHNVQIGADCLIQSSTVIGSDGFGYANDKGQWIKIPQTGGVIIGNRVEIGACTCID
                     RGALDPTVIEDNVIIDNLCQIAHNVHIGFGTAVAGGVIMAGSLKVGRFCQIGGASVIN
                     GHMEICDGAIITGMGMVMRPITEKGIYSSGIPLQTNKEWRKTAALVMNIDEMNKRLKS
                     LEKRFND"
     gene            11197..11655
                     /gene="fabZ"
                     /locus_tag="PXD66_000155"
     CDS             11197..11655
                     /gene="fabZ"
                     /locus_tag="PXD66_000155"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDL6318495.1"
                     /translation="MTTENREPNIIEVTEIMDMLPHRYPFLLVDRVTDYEEGKWLKAI
                     KNVTFNEPCFTGHFPGAPIFPGVLILEAMAQATGVLAVATYGKLSEDELYYFAAIDNA
                     RFKRPVVPGDQLQLEVEFLKEVRGITKFTGKAYVDGKLVCEADLMCARRK"
     gene            11689..12483
                     /gene="lpxA"
                     /locus_tag="PXD66_000156"
     CDS             11689..12483
                     /gene="lpxA"
                     /locus_tag="PXD66_000156"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HDL6318496.1"
                     /translation="MRLIDSTAKISPLAIIEEGAEIGAHVEIGPFSVIGKDVKIGART
                     KIHSNVVINGMTEIGEDNHIFQFASIGEINQDLKYQGEPTKVVIGNRNRIRESVTIHR
                     GTVQGGGVTKIGDDNLFMINTHIAHDCSIGNRCIIANNGTLAGHVTLDDFVIVGGMSA
                     IHQFVVIGSHVMLGGGSMVSQDVPPYVMAQGNHARPFGINFEGLKRRGFDKPTMHAIR
                     KVYKLIYSSGKTLEECLVEIEQIAATEPAIAIFKQFFKRSTRGIIR"
     gene            complement(12521..14521)
                     /gene="mnmC"
                     /locus_tag="PXD66_000157"
     CDS             complement(12521..14521)
                     /gene="mnmC"
                     /locus_tag="PXD66_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HDL6318497.1"
                     /translation="MGKLTFATLTFNDADTPVSEQFDDIYFSTQDGLAESYYVFQEGN
                     QLWEKWQSHPKEAFVIAETGFGTGLNFLAVADKFQQFRAAFPQSKLKRLYFISFEKFP
                     LTASQLADIHQRYPQFATLSENLTACWQPRQIGCQRYHFEQVYLDLWFGEIAENLPQL
                     GDLYSGTIDAWFLDGFSPDKNPEMWNEQLYLQMFRLSANGGSFATFTASSMVRRGLEA
                     AGFAVKKRKGFGKKREMLWGEKPLDSQVSEPNYPYFYAKNETADDIAIIGGGVASFFV
                     ALSLLEKGKQVTLYCKDETLAINASGNLQGAIYPQLSDDDERNVQFYIHSFDYALQRL
                     QQLKSNIEFEHNLSGVVLYAYDEKTAKKLEKMVEQTADDTLLKSCSQAELSEKLGLSV
                     PNSGAFIPQAGWLSPVQLVRNGFTWLEQKGLKIVLNHKVEHLTFSNGMWQWQTRGETV
                     RHQAVVLANGHTLNEFSQANGIPLYPVRGQVSQIPTTSELQKLKCVLCYDGYLTPMSA
                     YGTHCIGASHIRDNAETEFSLSEHWENVAKLQQNVTVCEWTQGIDLSENKAKVGIRAA
                     FRDRVPMVGAVHHFEQQKSQYANLYNQLRRKQAVENAEIFTNLYMVNGLSSRGLTTAP
                     LLGELLASLIVNEPVPISEDIWHNLSPNRAWIRKLLKGSTVA"
     gene            complement(14746..16182)
                     /gene="trpCF"
                     /locus_tag="PXD66_000158"
     CDS             complement(14746..16182)
                     /gene="trpCF"
                     /locus_tag="PXD66_000158"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HDL6318498.1"
                     /translation="MQNIVQDNAQPNQPTILQKIVQDKAIWVAQKEQAFPLSEFQHKI
                     VKSDHDFYAALAKGTHQEPAYILECKKASPSKGLIRAEFDLDSIAQVYKNYAAAISVL
                     TDEQYFQGDFRYIDQVKRQTTQPILCKDFMISAYQVYLARFYNADAILLMLSVVNDET
                     YRELATLAHELGMGVLTETSTEAEFERALALGAKVIGVNNRDLHTLTIDMNRIVRLVE
                     KYQAQISADVCLISESGINDHQQVKNIKPYVHAFLIGSSLMGSADLNNAVRAVIFGEN
                     KVCGLTRVQDVKAVYEQGALYGGLIFAEGSPRQLSLRQAQELVVNAPLRYVGVFQNQA
                     VEFVEKMAKQLELFAVQLHGSEDEQYIAELAEKLGSKTQIWKALSVDVEVENLEFNDN
                     PQIARYVLDSKVGNQQGGTGKTFNWALIPETLKQKALLAGGIGTENIEQALAQGCVGL
                     DLNSGVESAKGVKDLQKLTACFGKIIQA"
     gene            16270..16581
                     /locus_tag="PXD66_000159"
     CDS             16270..16581
                     /locus_tag="PXD66_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HDL6318499.1"
                     /translation="MKSQVIQHLSYLEIALRVNQLWEATPPSPEALANEQPFCVETLT
                     PTQWLQWIFIPRMRAILDANADLPRNFAITPYLEEALKNEGYLQAICEPLLGLEKLLK
                     D"
     gene            16735..17463
                     /gene="truC"
                     /locus_tag="PXD66_000160"
     CDS             16735..17463
                     /gene="truC"
                     /locus_tag="PXD66_000160"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HDL6318500.1"
                     /translation="MTLDILYQDNSLIAINKPAGVLVHRSWLDKHETVFAMQTLRDQI
                     GQHVFPIHRLDRPTSGVLLFALNSETARIMSEQFEQHLVQKTYLAVVRGYLQGSAQID
                     YPLKVILDKIADKFSQEKEAQAAITDYKGLATVEMPYPVGKHQTSRYSLVELSPKTGR
                     KHQLRRHLKHLFHPIMGDSKYGDLHQNRALVEKSGVSRLMLHAHKLQFQHPKNLQKIE
                     IIASLDEQWQRLFAFFDWNFTQYY"
     gene            17481..17645
                     /locus_tag="PXD66_000161"
     CDS             17481..17645
                     /locus_tag="PXD66_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HDL6318501.1"
                     /translation="MDNSLLSLTHEQQQAAVEEIQKLMAQGISAGEAIQIIANQLRDK
                     YQILEKSNNE"
     gene            17638..18114
                     /locus_tag="PXD66_000162"
     CDS             17638..18114
                     /locus_tag="PXD66_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318502.1"
                     /translation="MNKLWTVLALTITSHYSFAAAEKIVTKDLPAEGKPAVVLQVFDE
                     SEKIAKPIKGNKLFISKKQTRLCWHSINLPVQNKVTIAEAFYAPAAMKLASPGSKINS
                     SADKKNHTIITEINLANNQVLNRCWGFDKTDPVGKYKMEIQINDHIFKGLEFEIVK"
     gene            complement(18151..19002)
                     /locus_tag="PXD66_000163"
     CDS             complement(18151..19002)
                     /locus_tag="PXD66_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HDL6318503.1"
                     /translation="MENQKQAEIELKIMLEQQNVALVENYLNQTSPFPVLAHQTELLG
                     NTYYDTPEQFFATQKMGLRVRSVNQQYEITLKTQGEIVGGLHIRPEYNLALPNSQPDF
                     QTLVEKYQLPFENSEQIAESLQAIFSTDFTRKTWLVQVGMSDIEVALDQGIIKNHFGE
                     EPICELEFEIKQGNLGDLFTLVEAMPKADGMWLSNLSKAQRGYLLGQAVKWEQKIATA
                     MDGENSEKLTQLLADFICVGNESLEVLVRFNQCTQQQFSNWQQARAFVKSKDYLLHNL
                     TNLKTMI"
     gene            19063..20472
                     /locus_tag="PXD66_000164"
     CDS             19063..20472
                     /locus_tag="PXD66_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HDL6318504.1"
                     /translation="MFSKIHHKLNNFVQRGLDNHLRLAVTGLSRSGKTAFITSLVDQL
                     LHINKGGNSHLNLFAPARHGQILSVKRVEQGDLTIPRFEYDKNRQSLENQPPQWPEST
                     TGISEIRLAIRYQRQHSLLRHIKETGTLYLDIFDYPGEWLLDLPLLSLSYKEWSQTQQ
                     TIHKDERAELATPWLQAVKKLDLFAKTDENLLAEFSQQYTDYLLACKAQGMQYIQPGR
                     FVLPTENAKGAPVFQFFPLLDLSEAEWEKLEQSDRNSIFHTLKKRYEHYQKKIVKPFY
                     EDYFSQFDRQVILADCLTPLNYGYNEFMEMKLGLQQLFKHFHYGNRSLFHRLFSSNID
                     KLLFIATKADHITSDQVPNLESLMRQLVQEGGRHAEFDGIETGYLAISSIQATDAVKV
                     TQQDKIIKAIRGIRSSDRKQVTLYPGSVPNRIPDKNYWQFNRFEFDQFEPKQLDYSQA
                     IPHFRMDSVLQFLLADMFE"
     gene            20518..20868
                     /locus_tag="PXD66_000165"
     CDS             20518..20868
                     /locus_tag="PXD66_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL6318505.1"
                     /translation="MNTVISQRKPIHWVGSTLDDIREFPDNIKRELGFDLDLVQQGLL
                     PRDFKAMANLGSGVMEIRVKDPSGAFRLVYLAKFKDAIYCLHAFQKKTQKTAPKDIAV
                     IKARYAAVKELENE"
     gene            20861..21184
                     /locus_tag="PXD66_000166"
     CDS             20861..21184
                     /locus_tag="PXD66_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL6318506.1"
                     /translation="MNNIDTKIRHTTEAQGNVFEDLGFPIDEAQKLKSASQQLIETKL
                     MLMNEMSNWIDKNNLKQSEAASILGVSRPRISDMVNGKLEKFTLDTLVTFVAKTGKTI
                     QLQLS"
     gene            21292..21771
                     /gene="smpB"
                     /locus_tag="PXD66_000167"
     CDS             21292..21771
                     /gene="smpB"
                     /locus_tag="PXD66_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDL6318507.1"
                     /translation="MAKKPKVASNTIALNKRARHEYFIEDEIEAGLELQGWEVKALRA
                     GKGNISDSYVIFRHGEAFLFGAMITPLNMASTHIVSDPTRTRKLLLNKRELDSLFGKV
                     NREGYTIVATSMYWKNAWVKVKIGLAKGKKLHDKREDIKDREWQVAKQRIMKNANRG"
     gene            21974..23281
                     /gene="serS"
                     /locus_tag="PXD66_000168"
     CDS             21974..23281
                     /gene="serS"
                     /locus_tag="PXD66_000168"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDL6318508.1"
                     /translation="MIDQNLLRTNLEDVATALKIKRNFILDVERVKTLEEQRKTLQVK
                     TETLQAERNTRSKNIGAAKARGEDISALLAEVDSMGNELDTAKVELDKVQAEIRELLL
                     SVPNLPADEVPLGKDDSENLEVLRWGEPRQFDFEVQDHVTLGENLNGLDFAAGVKLTA
                     SRFVVMKGKLARLHRALSQFMLDLHTEQHGYVETNVPFLVNHDTLYGTGQLPKFGEDL
                     FHTQPLTGQDPNEVQRPFGLIPTAEVPVTNLVRDEIIDEDSLPLRYTAHTPCFRAEAG
                     SYGRDTRGLIRMHQFEKVEMVQIVAPEKSMEALEELTGHAEKVLQLLGLPYRKVLLCT
                     GDMGFGAAKTYDLEVWLPAQNTYREISSCSNMWDFQARRMSARCKAKGDKKTRLVHTL
                     NGSGLAVGRTLVAVLENYQNADGSITVPEVLRPYMGGLEVITA"
BASE COUNT         7335 a         4761 c         4670 g         6698 t
ORIGIN      
        1 taaatcgtat tgaacaagaa cttgcagact taggcattac aaatgtaaaa gaaatcgtgc
       61 gtaaccctag ctatgagcaa ctgtttgaag aagagatgaa atcggatctt aaaggttatg
      121 aaaaaggcgt cctaacaaat ttaggcgcag tagcggtaga taccggcatt ttcacggggc
      181 gttcgccgaa agataaatat atcgtattag atgaaacgac aaaagatacc gtctggtgga
      241 catctgatgc agctaaaaat gataataaac cgatgactca atctacttgg caaagtttaa
      301 aaaccttagt gaccgatgag ttatctaata agcgtttatt tattattgat gctttctgtg
      361 gtgcaagccc tgagcatcgc atcaatgtgc gtattattac ccaagttgcg tggcaagctc
      421 actttgtgaa aaatatgttt attcgtccaa cagaagaaga attaaaagat ttcaaaccgg
      481 attttgtggt aatgaacggt tcccaagtaa ctaacccgaa ctggaaagag caaggcttaa
      541 attcagaaaa ctttgtggca tttaatatca cagaaggtgt tcagatcatt ggcggtactt
      601 ggtacggtgg tgagatgaaa aaaggtatgt tctctatgat gaactacttc ttaccattaa
      661 aaggtgtcgc ttcaatgcac tgttcagcca atgtgggcga agaaggtgat gtggcggttt
      721 tctttggttt atcaggaacc ggtaaaacga cactatcaac cgatccgaaa cgcaaattga
      781 ttggtgacga tgaacacggt tgggacgaaa acggcgtatt taactatgaa ggtggttgct
      841 atgcgaaaac catcaacctt tctattgaaa acgagccgga tatttatcgt gcaatccgcc
      901 gtgatgcgtt attagaaaac gtggttgtgc gtgaagatgg cactattgat tttgatgata
      961 aatcaaaaac agaaaatacc cgtgtgagct atccgattta tcatattgat aatattgccg
     1021 tgccgtcaaa agcaggacac gctaaaaaag tgattttctt aacggcagat gcattcggcg
     1081 tattgccacc ggtgtctaaa ttaacgcctg aacaaactaa atactacttc ttatcaggct
     1141 ttaccgcaaa attagcaggt acagagcgtg gtattactga gccaacccca actttctctg
     1201 cttgtttcgg tgcggcgttc ttatcactcc acccgacaca atatgctgaa gtgttagtga
     1261 aacgtatgga agcagccggt gcggaagcct atttagtgaa taccggttgg aacggcacag
     1321 gtaaacgtat ttcgattaaa gatacacggg gtattattga tgccatctta gacggctcaa
     1381 ttgagaaagc ggaaatgagt acattaccaa tcttcaattt agcaattcca actgcgttac
     1441 cgggtgtaga aacccacatt ttagaccctc gtaacactta tgaagacaaa gcacaatggc
     1501 aagctaaagc agaagattta gctggcagat ttgtgaaaaa ctttgaaaaa tatgcgacca
     1561 acgaggaagg caaagcatta attgctgctg gtccgaagtt ataagttgta attataagtt
     1621 ataatcgcta aaaaattagt ttaggactaa accttgaatg aggtttagtc cttttgtata
     1681 aaggagaagt acaataatga caacattcca attaaggctg aaagccaaag atgagcttac
     1741 cccacaccca acagcacaat attggcgaaa atgccaagtg gaaggactat ttgatctgcc
     1801 gtttatggat ttagtgttta gagcggcaca agttcaccgt gaaaattttg atgctaatgc
     1861 gatccagctt tcaacgttac tctcaattaa aacaggaggc tgtccggaag attgcgaata
     1921 ttgcccccaa tctgcccgct atgatacggg aatagaaaag caaacaatgc ttgatgtaga
     1981 gacgattgta gaaaaagcta aaattgccaa agagcgtgga gcaagccgct tttgtatggg
     2041 ggctgcgtgg cgaggaccta ggcctaaaga tattaaagta gtctcagaaa tcatcaatgc
     2101 ggtgaaatca ttggggctag aaacctgtgg tactttcggt atgcttgaag agggaatggc
     2161 agaagaatta aagcaagcag ggctggatta ctacaaccat aatttagata ccgaccctga
     2221 acgctataac aaaattatcc atacccgaaa tcacgaagat cgaatggaca ctttaggtaa
     2281 agttcgcaat gccggcttga aagtttgctg tggcggtatt gtgggaatga acgaaacccg
     2341 ccccgagcga gcaggcctaa ttgcaagcct cgctaacctc gatcctcaac ctgaaagcgt
     2401 accgattaat cagttgatta aagtagaagg tacaccgctt accaatgccg aagatttaga
     2461 ctggacagaa tttgttcgta ccattgcggt agcaagaatt actatgcctc aaagctttgt
     2521 ccgactttct gctggcagaa ctactatgcc ggaggcaatg caagccctct gttttatggc
     2581 aggagcaaat tctattttct atggcgataa attacttact acagaaaatc cggaagaaga
     2641 tcatgatcgc caattaatgg ataaattaga tttatatccg ctacaatata tagcctgagt
     2701 gccaatgatt ttcgcctgaa tgaaaattaa agaatcaatc gttattttac gcaaatttct
     2761 aacataaaat aaccgcttgc aaaattcgtt cttgtcttat ttttaaattt atctataatg
     2821 tgaggcctta cgcaagcttg cgtaattcga tggggcgtta ttggttcctc gcaatggtgt
     2881 tcagcttact tgatcaggtt cggaagaaag cagtctgggt tgaaatttcc gtgtgccgag
     2941 ggaggctggt aatgccccac ccttttttca gttctatcag gagaacgtta tgctccagat
     3001 cacccccatt ccggcattaa aagataatta catttgggca atccgtaacg ggcaagatgt
     3061 gattattatt gatccatcag aacatcagcc agcattaaac tttattgcaa aaaatgcgtt
     3121 aaatttaacc gctatcttga ttacccataa tcaccacgac catactgatg gggtgagtgg
     3181 tattgttgag caatatccga atatccctgt ttatggtccg caagaagtgg ctgaatttgc
     3241 caatgtgatt gtctcgcctg aacaacatat cagcctgttt ggttatgatg taaggattat
     3301 tgaaagtggt gggcataccg cagggcatat cagttattta tttggctatg attatctgtt
     3361 ttgtggcgat gcgttattct ccggcggttg cgggcgggtt ttcaccggtg attaccaagc
     3421 ccaatttgat gcgttacaac gctttaaaaa tctgcctaac tcggttaaag cctacccggc
     3481 tcacgaatac accaagagca atttgaaatt tgcagaagcg gttctaccac caagctgtgt
     3541 attatcggaa tatcaagaac aggttgatct tttacgttcg caaaacaaac cgactttgcc
     3601 aaccactatt ggcttggaaa tgcaggtgaa cccgtttatg caagcggtcg atttagccga
     3661 atttattgca cttcgtcagc aaaaagacaa tttttaacct catatttcta attaagcatt
     3721 aaagcctctc actttaatgc tttttggtta aataaaacct ttgcaaacat cgcttgtcaa
     3781 ttccactctg gcttatccta tggtatagtt acgactattt ttagcttaat ttattatttt
     3841 agtgagtaaa attctgtgaa cttcgatcct caaaatatgc ctaagcacgt tgccattatt
     3901 atggatggca atggcagatg ggctaagcag aaaggaaaat tacgcatttt tggtcatcag
     3961 aatggtgtaa aggcagttcg ttctgcagtt agttttgcag caaagcatca tatccaagtt
     4021 ttaaccctct acgcatttag cagtgagaat tggagcagac cagaaactga agtttcagct
     4081 ttaatgtctc tgtttatgaa agctttagat tctgaagtta aacaattaca taaaaataat
     4141 attcgcttaa agattatagg cgataaatcc gcctttagcg aaaatttaca aaaacgcatt
     4201 gccgaatcgg aaaaattaac cgaaaacaat actgggctaa tcttaaacat tgctgccaac
     4261 tatggtggtt actgggatat tgttcaagct gctcaacaac tagcagtgca cgttaaatct
     4321 gaacaacttg ctgtggagca gattacacca gcattgtttc aacaacattt agtgacagaa
     4381 aaccagccgc aagtggattt attaattcga accagtggtg agcagcggat cagcaatttt
     4441 ctattatggc aaattgctta tgccgagctg ttttttagcc cggttttatg gcctgatttt
     4501 aatgatgagg cttttagtga tgcaatttat gcttatcagc aacgtgaacg ccgattcggt
     4561 ggttgttaat ataaaaagaa taaggaaata caatgcttaa agaacgtgta ctttcagcga
     4621 ttattatgat tattgccgtg ctggctgcca tattttggct ctcaccactt cctttaactt
     4681 tagtgttatc ggcaattgtt gtggctgcaa tgtgggaatg ggctcaattt gccggcttta
     4741 aacaccctgt gccacgtgca attgtggcaa tggtgacaat ctgcttatta ctttttgcga
     4801 tcgttgcgaa tactgattat atccgagcca cccgttttat tactgatgag actaccccta
     4861 tcctctttat tggctgtatt tggtggttaa ttgcccttgt cctagtggca acttacccca
     4921 actcggcaaa actgtgggaa aaatctgttg cagctaaatt cctatttgga cttgctacct
     4981 tagtgccgtt tttaattggc acgctcgcat tgcgtttcca taattatgca tttgatccat
     5041 accaaggaac ttatttattc ctttacgtct gtttattagt ttggggagct gattcaggtg
     5101 cttattttgc tggcagagcc tttggcaaaa gaaaacttgc cccaaaagta tctccgggta
     5161 aatcgtggga aggcgtaatt ggtggcgtac tcacctctag tttgattgcc tttacctttt
     5221 tacaacttac accggcaaat gtattcggca aagcattagc cactacccca tttattttag
     5281 tttcggtcgg tatggttgct atttcagtct taggcgattt ggcagaaagt atgtttaaac
     5341 gccaagcagg cattaaagac agtagccagt taattcctgg gcatggaggt attttagatc
     5401 gtattgatag tttaaccgct gccgttccgt tttttgctac attcttcttt tttgtactat
     5461 agaatatgac gtccatcatt gcatttttta ttttaatttg cgtactggtt tttgtgcatg
     5521 aatacgggca cttttgggct gctcgcaaat gcggggtaaa ggtgatccgt ttttcgatcg
     5581 gatttggtaa agtcttattt aagaaaaaag ataaacaagg aaccgaattt gttttttcac
     5641 ttattcctct gggcggctat gtacaaatgt ggaatgatga aacggatatc aacgctccag
     5701 ctcaacaagc tcttaacaca aaatctgtat tgcaacgtgc atttattatt atagccggtc
     5761 ctgctgcaaa ttttattttt gcgattttgg catactgggt cgtttttatt gccggtattc
     5821 caactgttaa acctgttatt ggggaaattt taccaaacac tatcgcctcc caagcaagaa
     5881 ttcctaccga attggaaatt acaaaaatcg gtcatcaatc tatccaagat tgggaaagtg
     5941 catctttagc actagttggt tttgtgggca ataaaaatgt accgttagag ggaaatatca
     6001 acggcaactc aacacaaaaa tatgaactcg atttatctaa ctggagtatt gactcagcca
     6061 aagaaaaccc attaaccgca ttaggtattc gccccaaaag tgcaaaagtc accgctgaaa
     6121 ttaaacaagt tgtcgaaaat tctcctgcaa gccaagtggg tattcaagca ggtgatcgta
     6181 ttcttcaaat taaccagcag ccatttaatt ggttcaattt agttgaactg gtacaagcag
     6241 gtaaacctat tgaacttaaa attgaacaac gaggtcagat taaagacctt gtcgtccaac
     6301 cagaaaaaaa agacgaacgc tatattatcg gggtaatacc aagttatgag cctcttgcag
     6361 ataaatatcg taccgaatta aaatatgata ttcttaccgc attttataaa agtatcgaaa
     6421 aagtgtggtc tcttacccaa accatactcc aatttatcgg taatttaata agcggtgatt
     6481 tgtccattaa aaatttaggt gggccaattt caatggcaaa aggagccggt gcaacagctg
     6541 aaatcggctt agtttattac cttagtttta tggctctaat cagtgtcaat ttgggggtaa
     6601 tgaacttatt tccactttta cccttagatg gtgggcaact tgttttattg gctgttgaag
     6661 caattcgtgg aaaagcatta tcagaaaaaa tacaactcaa attccaacaa attggatttg
     6721 cattcgtgct gagtttaatg ttattcgcct ttgccaacga tataattcac ttttaacaag
     6781 cggttaaaaa aggtgacttt tttgcaaaaa gcaccctgac atcaaaatcc ataatatgga
     6841 tttaaactat attcttaata attttgcaaa acgatcatag gataatttca atgaaaaaat
     6901 tattactttc ctctctttta attgcaaacg gtgtagttgc tgcacctttc gtagtaaaag
     6961 acattcgtgt tgaaggtgtg caacctacaa caggcgaatc tatcatttca tctattccgg
     7021 ttagagtagg tcaaacagca acggatactg acgtatctaa tgtcgtccgc catctattca
     7081 gccaacaacg ttttgcagat gttcgtgcaa cacgagaagg caatacgctt gtcatcaaag
     7141 tcgctgaaaa accgattatt ggcaaggtag agatcgaagg taaccaagcg attcctaaag
     7201 atgccttaga gcaaaattta aaggcaaact taattaacca aggtgaaatt tttgatgctg
     7261 ccaagcttga agcttttaaa gaaggactaa tcgaacatta tcactctacc ggccgttatg
     7321 aagccaaaat tgacacatct gtttcgaata acaccgaagg tggcgttaat attaagttag
     7381 cgattaatga aggtgaagtt gctaaagcca aaacaattaa gtttgaaggt aataatgcct
     7441 tctctgataa aaaattatta gaaaacttag atattcaacc tgatgtttct tggtggaata
     7501 tttttgaaag tagcaaattc gaacaaaccg cctatcaaaa agacttagaa acattaagag
     7561 atttttattt aaatcgtggc tatgcaaaat ttgctatcca agataccgat gttcaattca
     7621 atgataataa aacggaagtt gcattgacct ataaattaga tgaagggtca cagtaccatg
     7681 tcagtgaaat gcgcattatc ggtaatacag ccaatatgga tagccagctc aacgccctac
     7741 taaaagaatt caagcctggt caattattcc gtaaatcaga ccttacacaa attgaagaag
     7801 agattaaatc tattttaggg gacgcaggtt atggttcagc taaagtagat atttatcctc
     7861 aatttaatga agaaaataaa accgtaaaaa tcagttttgt tgtggatgcc ggtcgtcgta
     7921 tttatgttcg taaaatccgt tttgaaggta atggtgtcac tgcagattca acattacgtc
     7981 gtgaaatgcg ccaacaagaa ggtgcttggc tttccacaaa caaagcttct ctaggtaaag
     8041 ctcgtttaga aagaaccggt ttctatgaaa gtgttgatat gtcaatgcca aatgtagaag
     8101 gtacagccga tcaagttgat gtagtgtata aaattaaaga acgcaatacc ggtagtatta
     8161 actttggtat cgggtacggc acggaaaacg gtatcagcta tcaagcagga attaaacaag
     8221 ataatttctt aggtatgggg tcaactatca gtctaaatgg tacccgtaat aactacggta
     8281 ccagcgttaa cctaggctat accgaacctt actttactaa agatggtgta agtttaggcg
     8341 gtaatatttt ctatgaagat tatgataact cagacaatga ttcaatggct tcatataaac
     8401 gccaaaccta tggtattaac ggcacattag gcttcccggt aaatgaaaat aactcatact
     8461 acttaggttt aggctatacg cacgataaaa ttaaaaatgc agcacgtgag tacactcgtg
     8521 agaaatatgt aaaatcaatg aactttgagt tcgatccagc tgccgattat tacaaaaaaa
     8581 tcaaagctga tgattttgat ttttctctcg gttggaacta caacagcttg aatagaggct
     8641 atttcccgac tgaaggaact accgcaaaca ttggcggtaa aatcacaatt ccaggctctg
     8701 ataataaata ttaccgagtc agtgcagact ttagaaatta ttacccgtta aaccgtgagc
     8761 acaaatgggt aatttcaacg aaagctagca ttgcttatac taatggattt ggtggtaaag
     8821 aagttccgtt ctatcagctt tatactgccg gaggtattgg tacactaaga ggtttctctt
     8881 acggtgcagt agggcctcaa gcgatttact ataatacaaa atccggcaaa tttgatagca
     8941 tgaataatga tattatcggt ggtaatgcgt tagctgcggc aagcttggaa ttaatcacac
     9001 caacgccatt tgtgagcgaa aaatatcagc acaatgtaag aacctctctc tttgttgatg
     9061 ctgcaagtgc atggaataca aaatggaaga aagatgaata tacgaacttg cctgactatg
     9121 gtgatttcaa acgtttccgt gcttccgccg gtattgcatt ccaatggatg tctccaatcg
     9181 ggccattatc attctcttat gcgaaaccaa ttcgtaaata tgataatgat gatattgaac
     9241 aattccagtt caacattggt agttcattct aattaaccac aagcggttaa taaatgtaaa
     9301 aaatttgcaa cttagccgct tatttcaaaa ctgttcatac aaggaaaaca aatgaaaaaa
     9361 ttatttaaaa tgactggctt agccgtagct ttagcctcta caaccggaat cgcgaatgct
     9421 gcagatacta ttggtttcgt agatccaagc tatgtattac aaaaccaccc tgtattatta
     9481 gatgcttcgg caaaatttga taaattcatg aaagaaagcc aagaaaaatt tgctgctgat
     9541 gaaaaaaaat tagcagatga aaataaaacc ttaacggctg agcgcaataa aattaatgaa
     9601 gatgcgcaaa aattacaaaa tgaacaaaaa aatgtagaag cttctatcaa gaaaaaagtg
     9661 gcagcattag agaaagatgc gcctcgctta cgttcaaaag agattcaagc acgtcaaaat
     9721 gcgattcaaa aagaagcaaa cgcattccaa gctaaagtaa cagcaattca aaaacgtgaa
     9781 gctgattttg caaaaaaagc agaagccttc cagaaacgtg cagatgaatt ccaaaaacgt
     9841 attgaacaag cgaataaaga aaatggtggc attaacccac aagaagcgca aaaacaagcg
     9901 gttgatgaag taaatgcaac cattaaagag atcgcaaaat cgaaaggtta cactttagta
     9961 ttacaaccac aagtagcact ttatgctgaa gatgaaggta aagatattac tgaagcagta
    10021 cttgatgcta ttgttaaaaa acatccggaa attaaaatag aaaaacctgc aactgaagct
    10081 caaccagcta cagaaaaaga aaccaaagct gaagaaacta aaccagaaga agcgaaaaaa
    10141 taatggcaaa tttccgttta attgatttag cggagcatat cggcggtact cttaggggta
    10201 acgccgattt ggctattcaa agtattgctg ctttagataa agccgacagc tcgcaaatta
    10261 cctttatctc aaatgcaaaa tatcgggaaa atttgaccgc ttgcaatgct ggagctatta
    10321 tcgtcagccc ggcagatgta gaattttgcc gagcagatca aaatcttatc atcgttgcca
    10381 atccgtatgt tgcttatgct caactggctc aatatatgga ttctactccg aaagcagcga
    10441 gcgaaatcca cccaagtgcg gtgatttcac ctgaagctaa actgggtaat aacgtatcgg
    10501 taggggcaaa tgccgtgatt gaaagtggcg tagaattggg cgatgatgcc gtgatcggtg
    10561 caggctgttt tgtgggaaaa aacagtaaaa tcggggcaag aacccaactt tgggcaaatg
    10621 tttcggtgta ccacaatgta caaatcggag ctgattgctt gattcaatcc tcaacggtta
    10681 tcgggagtga tgggtttggc tatgcgaatg acaaagggca atggattaaa atcccacaaa
    10741 ccggtggtgt gattatcggt aatcgtgttg aaattggtgc ttgcacttgt attgaccgag
    10801 gtgcattaga tccaaccgta atcgaagata atgtgattat tgataacctt tgccaaatcg
    10861 cccataatgt acacattggt tttggcactg ccgttgccgg cggcgtcatt atggcaggca
    10921 gtctaaaagt tggtcgcttt tgccaaattg gtggagcaag tgttatcaac ggtcatatgg
    10981 aaatctgtga tggtgccata attaccggaa tgggtatggt tatgcgccca attacagaaa
    11041 aaggaattta ctcatcaggt attccattac aaaccaataa agaatggcga aaaaccgcag
    11101 cacttgtaat gaatattgat gaaatgaaca aacgcttaaa atcgttagaa aaacgtttta
    11161 acgattaatt ttattagagg tcttaaagtg acagaaatga caactgaaaa tcgtgagcca
    11221 aatattattg aagtaaccga aattatggat atgttaccgc atcgctaccc tttcctgtta
    11281 gttgatcgcg taaccgatta tgaagaaggg aaatggttaa aagccattaa aaatgtaacc
    11341 ttcaatgaac cttgttttac cgggcatttt cctggcgcac caatcttccc aggagtattg
    11401 attctggaag caatggctca agcaactggc gtattagccg ttgccactta tggtaagtta
    11461 agtgaagacg aactctacta ttttgccgca attgacaatg ctcgctttaa gcgaccggtt
    11521 gtgccgggcg atcaacttca acttgaggtt gaatttctta aagaagtacg tggtattact
    11581 aaatttaccg gtaaagcata cgttgatggc aaattagtct gtgaagcaga tttaatgtgc
    11641 gctcgccgta aataattcga tataagccca aactagaggg aacaaactat gcgtctaatt
    11701 gattcaacag caaaaattag cccgcttgcc atcattgaag aaggtgctga aattggcgct
    11761 cacgttgaaa ttggtccatt ttcagtcatc ggtaaagatg tcaaaatcgg ggctagaacg
    11821 aaaattcact caaatgttgt cattaacggc atgactgaaa ttggtgaaga taaccatatt
    11881 ttccaatttg ccagcattgg tgaaatcaac caagatctaa aataccaagg tgaaccaacc
    11941 aaagtggtga tcggtaatcg taaccgtatt cgtgaaagtg ttactattca ccgaggtacg
    12001 gtacaaggcg gtggcgtgac gaaaatcggt gatgataact tatttatgat caacacccac
    12061 atcgcacacg actgctctat cggtaaccgt tgtattatcg ccaataatgg tacgctagca
    12121 ggtcacgtta ccctagatga tttcgtgatt gtgggaggaa tgtccgccat tcaccaattc
    12181 gttgtgattg gttcacacgt tatgcttgga ggcggttcaa tggtcagcca agacgtaccg
    12241 ccttatgtca tggcacaagg taaccacgca cgcccatttg gtattaactt tgaagggtta
    12301 aaacgtcgtg gctttgataa accaactatg cacgctattc gcaaagtcta taaactgatt
    12361 tactccagcg gaaaaacatt ggaagagtgc ctagtagaaa ttgagcaaat tgcagcaaca
    12421 gaacctgcga ttgctatttt taaacaattc ttcaaacgtt ctacccgtgg tattattcgt
    12481 taataaacga aaccccgaaa tcaattcggg gtttgtttta tcaagcgacc gtcgaacctt
    12541 tcaacaattt tcgaatccaa gctcgatttg ggctaaggtt atgccaaata tcctcactga
    12601 ttggcacagg ctcattcaca atcaggcttg ctaacaattc ccctagaagt ggtgcagtcg
    12661 tcaaacctcg agaagacagg ccgtttacca tatacagatt ggtaaaaatt tcagcatttt
    12721 ctaccgcttg tttccgtctc aactggttgt ataaattggc atattggctt ttttgctgct
    12781 caaaatgatg cactgcccca accatcggta cacgatcacg aaacgccgca cgaataccga
    12841 ctttggcttt attttcagat aaatcaattc cttgcgtcca ctcacaaacg gttacatttt
    12901 gctgaagttt tgcaacattc tcccaatgct ccgataaact aaactcggtt tctgcattat
    12961 cccgaatatg gcttgctcca atgcaatgcg tgccataggc tgacattggc gttaaataac
    13021 catcataaca aagcacacac ttcagttttt gtaattcgct tgtagtcgga atttggctca
    13081 cttgccctcg taccggatac aacggaatgc cgttcgcttg gctaaattca ttcaacgtat
    13141 gtccatttgc cagcacaacc gcttgatgtc gaaccgtttc tcccctcgtc tgccattgcc
    13201 acattccatt agaaaaggtt aaatgctcaa ctttgtgatt cagtacaatc ttcaaccctt
    13261 tctgctccag ccaagtaaag ccatttctaa caagttgtac cggtgaaagc caaccagctt
    13321 gcggaataaa tgccccgctg tttggcacgc ttaaacctaa tttttcactt aattctgcct
    13381 gtgaacaaga ttttaataag gtgtcatcag ccgtctgctc caccattttt tccagttttt
    13441 tagccgtttt ctcgtcatag gcgtaaagca ctaccccgct taaattatgc tcaaactcaa
    13501 tgttggattt cagttgttgc aagcgttgca atgcataatc aaaactatgg atataaaact
    13561 gtacatttcg ctcgtcatca tcgcttaact gtgggtaaat cgccccttgt aaatttcctg
    13621 aagcatttat tgccaaggtt tcatctttgc aatacagcgt gacttgcttg cctttttcta
    13681 acagcgataa cgcaacgaag aaactcgcta ctccgccacc aataattgca atgtcatcag
    13741 cggtttcatt tttggcataa aaataggggt aattaggctc ggaaacttgg ctatctaacg
    13801 gtttctcgcc ccaaagcatt tctcgttttt tgccaaatcc tttgcgtttt ttcactgcaa
    13861 aacctgcggc ttccagccct cgtctgacca tacttgaagc ggtaaaagta gcaaaactgc
    13921 cgccattggc acttagtcga aacatttgca gatataactg ttcgttccac atttctgggt
    13981 ttttatcagg cgaaaagcca tctaaaaacc aagcgtcaat cgtgccgcta taaagatcgc
    14041 ctaattgggg taagttttca gcaatctcac cgaaccataa atctaaataa acctgctcaa
    14101 aatgataacg ttggcaacct atttggcgag gctgccaaca agcggttaaa ttttcagata
    14161 atgttgcaaa ttgcggatag cgttggtgaa tatcagctaa ttggctggcc gtcagaggaa
    14221 atttctcaaa tgagataaaa tagagtcgtt ttagtttact ttgcggaaat gctgcccgaa
    14281 actgttggaa tttgtcagca acggctaaaa aattcaagcc cgtgccaaag ccggtttccg
    14341 caataacaaa agcctctttc gggtgagatt gccacttttc ccacagttgg ttaccttctt
    14401 gaaacacata gtaactttcc gccaaaccat cttgggtaga aaaataaata tcatcaaatt
    14461 gctccgacac cggtgtatct gcatcattaa atgtaagtgt cgcaaaagtc agtttaccca
    14521 tcatatcccc ttaaaaacaa aacacggaaa accccaagat tttccgtgtt tcaaccagtt
    14581 caatagtacc aatgttagaa aatgttgctg gcaaaaataa cgatcatacc aagtgcgcct
    14641 acaaccgtta gcattgaata accaaaagta cccattttac aatcctcatt gttaattatg
    14701 attaaaacgg ctttattcta gcataatttc catcgctttg aatgcctatg cttgaataat
    14761 tttgccaaaa caagcggtca atttttgtaa atcttttacg cctttggcac tttccacgcc
    14821 agaatttaaa tctaacccga cacagccttg agcaagggct tgctcaatat tctcagtgcc
    14881 aataccacct gccaataacg ctttttgttt taaggtttct ggaatcaacg cccaattaaa
    14941 ggttttgcct gtgccgcctt gctgattgcc tactttgctg tctaatacat aacgagcaat
    15001 ctgtggatta tcgttaaatt ctagattttc aacctcaaca tcaaccgata aggctttcca
    15061 gatttgagtt ttactaccta atttctctgc cagttctgca atgtattgct catcttccga
    15121 gccgtgcaat tgaactgcaa ataactcaag ctgttttgcc attttttcca caaattcgac
    15181 cgcttgattt tggaacaccc ccacataacg caacggtgca ttcaccacca attcttgggc
    15241 ttggcgaagt gaaagctggc gaggcgagcc ttcggcaaaa attaaaccgc catataatgc
    15301 cccttgctca taaaccgctt tcacatcttg cacacgggtt aagccacaca ctttgttttc
    15361 accaaaaatc accgcccgca ccgcattatt gagatcggca cttcccatta aactgctgcc
    15421 gattaaaaaa gcatgaacat acggcttaat gtttttcact tgctggtgat cgttaatacc
    15481 cgattctgaa atcaaacaga catcagccga aatttgggct tggtattttt ccaccaaacg
    15541 cacaatgcgg ttcatatcaa tcgtgagcgt gtgcaaatca cggttattaa cgccaattac
    15601 cttcgcccct aaggctaagg cacgttcaaa ttccgcctcc gtgctggttt cagtcaaaac
    15661 gcccattcct aactcgtgag ccagtgtggc aagttcacgg taggtttcat catttaccac
    15721 cgataacatc aataaaatcg catcagcatt ataaaaacgg gcaaggtaaa cctggtatgc
    15781 cgaaatcata aaatctttac acaaaatcgg ctgtgtggtt tggcgtttga cttgatcgat
    15841 ataacggaaa tcgccttgaa aatattgctc atcggttaaa accgaaatcg ccgcagcgta
    15901 atttttatac acttgggcaa tactgtctaa atcgaactca gcacgaatta acccttttga
    15961 cggtgaagct tttttacatt ctaaaatata agctggttct tggtgtgtgc ctttggcaag
    16021 agccgcatag aaatcatggt cgctttttac aattttgtgc tgaaattcac ttaacggaaa
    16081 ggcttgctct ttttgtgcca cccaaatggc tttatcttgt acgatctttt gtaaaatcgt
    16141 cggttggttt ggttgagcgt tatcttgaac gatgttttgc atataaattc ctagagcgaa
    16201 atcggtttga atggtaaaat ctgcccttat ttttacccga aaaagtctca tttcacaagc
    16261 aaaactaaga tgaaatcaca agttatacag catttatctt atttagaaat cgccctgaga
    16321 gttaatcaac tttgggaggc gacaccacca agccctgaag ccttagccaa tgagcaacct
    16381 ttctgcgtgg aaaccttaac cccaacccaa tggctacaat ggatttttat tcccagaatg
    16441 cgggccattt tagatgcaaa tgccgattta cctcgcaatt ttgcaatcac cccttattta
    16501 gaagaggcat tgaaaaacga aggttattta caagcgattt gtgaaccgct attaggtttg
    16561 gaaaagttgt tgaaagatta acttctctct cagttctttt attaatctga caaattcgtt
    16621 gatagtgcca gcgtccacgc tggtactaat tttaacggtt aattaatcaa aatttggcac
    16681 aagcgaggac gcttgcgcca tcatagagat ttgataaatt atacttaatg tattatgacc
    16741 ttagatattt tataccaaga caactcgctt atcgccatca acaaacctgc cggagtgttg
    16801 gtgcatcgtt cttggttaga taaacacgaa acggtgtttg caatgcaaac cctgagggat
    16861 caaatcgggc aacacgtttt tccgattcac cgccttgacc gcccaacctc aggtgtattg
    16921 ctgtttgctc tcaacagcga aaccgcccga ataatgagcg agcagtttga acaacaccta
    16981 gtacaaaaaa cttatttggc ggtggtgcgt ggctatttac aaggttcggc acaaattgat
    17041 tatccgctca aagtgatttt agataaaatt gcggataaat tttctcagga aaaagaggct
    17101 caagcggcga ttactgatta taaaggctta gcaacggttg aaatgcctta ccctgtcggc
    17161 aagcaccaaa cctctcgtta tagtttggtt gaacttagcc ctaaaaccgg gcggaaacat
    17221 cagcttcgcc gccacctgaa acacctgttc cacccgatta tgggagacag caaatatggc
    17281 gacttacacc aaaaccgtgc tttagttgaa aaatcaggcg taagtcgttt aatgttacac
    17341 gcccataaat tgcaatttca gcacccgaaa aatttgcaaa aaatagagat aattgcatcg
    17401 cttgatgagc aatggcaacg cctctttgca ttttttgact ggaactttac tcaatattat
    17461 tagacaaaaa agaggattga atggataatt cacttttatc attgacacac gagcaacaac
    17521 aagccgcagt agaagaaatt caaaaactga tggcacaagg catcagtgcc ggagaggcaa
    17581 ttcaaattat tgctaatcaa ttacgtgaca aatatcaaat attggagaaa tcaaacaatg
    17641 aataaattat ggacagtgct tgccctaaca ataacttccc actatagctt tgctgccgct
    17701 gaaaaaatag tcactaaaga tttaccggca gaaggaaaac ctgcagtcgt gctacaggtt
    17761 tttgatgaaa gtgaaaaaat tgcaaaacct attaagggaa ataagctctt tattagcaaa
    17821 aaacaaacaa ggctttgctg gcacagtatt aatcttcctg tacaaaataa agttactatt
    17881 gcagaagctt tctatgcccc tgctgcaatg aaattagctt ctcccggctc taaaattaat
    17941 tcttctgcag acaagaaaaa tcatactatc attactgaaa taaaccttgc aaataatcaa
    18001 gtattaaatc gttgctgggg ttttgataaa actgaccctg tcggtaaata caaaatggaa
    18061 attcaaatta acgatcacat tttcaaaggt ttagaatttg aaattgtaaa ataacctaaa
    18121 agccacagag taacaccctg tggctttttg ctaaatcatt gtttttaaat tggttaaatt
    18181 gtgcagcaaa taatctttac tcttcacaaa ggctctcgcc tgttgccagt tgctaaattg
    18241 ttgctgagtg cattgattaa aacgtaccag cacttccaaa ctttcattgc cgacacaaat
    18301 aaaatcagct aataattggg ttaatttttc actgttttca ccatccattg cagttgcaat
    18361 tttctgctcc catttaaccg cttgtccgag caaataaccc cgctgggctt tgcttaaatt
    18421 actcaaccac atcccatccg cttttggcat tgcctcaacc aaagtaaata agtcgcctag
    18481 atttccttgc ttgatttcaa actccaattc acaaatcggc tcttcaccaa aatgattttt
    18541 aatgatgccc tgatctaacg ctacctcaat gtcagacatc cccacttgca ccaaccaagt
    18601 tttacgagtg aaatcggtgc tgaatatcgc ttgcaagctt tctgcaatct gctctgaatt
    18661 ttcaaacggc aactggtatt tttccactaa tgtttgaaaa tccggctggc tattgggtaa
    18721 agctaaatta tattccggac gaatatgcaa accgcctaca atttcccctt gtgtttttaa
    18781 cgtgatttca tattgttgat tcacagaacg cacccgcagc cccatttttt gcgtggcaaa
    18841 aaattgctcg ggcgtatcat aataggtgtt gcctaacaat tccgtttgat gagcaagcac
    18901 agggaatggc gaggtttgat tcaagtagtt ttccactaac gccacattct gctgttccaa
    18961 cattattttc agttcgattt ctgcttgttt ttggttttcc atttcacacg cccctgcaac
    19021 gttgtataat caagcggtta ttttatcaat aaattttgca aaatgttttc aaaaattcac
    19081 cataaactga ataactttgt ccagcgtgga ctcgacaacc atttacgcct cgccgttacc
    19141 ggcttaagcc gtagtggcaa aacggcattt attaccagct tggttgatca gcttttgcat
    19201 atcaacaaag gcggtaattc gcatttaaac ctgtttgcgc ctgcacgcca cgggcaaatt
    19261 ctatcggtaa aacgcgttga gcaaggggat ttaactattc cccgctttga atatgataaa
    19321 aaccgccaaa gccttgaaaa tcaaccacct caatggcctg aatccaccac tggcattagc
    19381 gaaattcgcc ttgcgattcg ctaccagcgg caacattctc tgctacgtca tattaaagaa
    19441 accggcacgc tttatttgga tattttcgat tacccgggcg aatggctgtt agatttaccc
    19501 ctgctctccc tttcctataa agaatggtcg caaacgcaac aaaccataca taaagatgag
    19561 cgtgcagagc ttgccacacc ttggttacaa gcggttaaaa aactcgatct ttttgcaaaa
    19621 accgatgaaa atttactggc agaattcagc caacaataca ccgattattt gctcgcctgc
    19681 aaagctcaag gaatgcaata tattcagccg gggcgatttg tgttgccaac tgaaaatgcc
    19741 aaaggtgcac cggtattcca attttttccg ttattagatt taagcgaagc tgagtgggaa
    19801 aagttggaac aaagcgacag aaacagtatt ttccataccc tcaaaaaacg ctatgagcat
    19861 taccagaaaa aaatcgtcaa accgttttat gaagattact tctctcaatt tgaccgccaa
    19921 gtgattttgg cagactgcct cacaccgtta aattatggct ataacgaatt tatggaaatg
    19981 aaattagggt tacagcaact gtttaaacat ttccattatg gcaatcgttc gctatttcac
    20041 cgtctgtttt cgtccaacat tgataaattg ctatttatcg caacaaaggc agatcacatt
    20101 actagcgacc aagtgccgaa tttggaaagc ctaatgcgac aattagtgca agaaggggga
    20161 agacacgcag aatttgatgg tatcgaaacc ggctatttag ccatttcctc gattcaagca
    20221 accgatgcgg tgaaagtgac tcagcaagat aaaatcatta aagcgataag agggatccgt
    20281 tccagcgata gaaaacaagt gacactctac cctggcagcg tgccaaatcg catacccgat
    20341 aaaaattact ggcaatttaa ccgttttgaa ttcgaccaat tcgagcccaa gcaacttgat
    20401 tatagccaag cgattccaca cttcagaatg gattcggtgc tacaattttt attggcggat
    20461 atgtttgagt aaaatcttgt tgcatatttc cattaaaggt ataaaatttt atacattatg
    20521 aatactgtaa tttcccaacg aaaaccaatt cactgggttg gatcaacctt agatgatatt
    20581 cgtgaatttc cggataacat taaaagagaa ctcggttttg atcttgattt agttcagcaa
    20641 ggcttattac cgagggattt taaagcgatg gcaaatttgg gttcaggcgt aatggaaatt
    20701 cgagtaaaag atccctcagg ggcttttcgc ttagtgtatt tagctaaatt taaagatgca
    20761 atttactgtt tacacgcttt ccaaaagaaa acgcaaaaaa cagcccctaa agacattgct
    20821 gtcattaaag cacgctatgc tgccgtaaag gagttagaaa atgaataata ttgatactaa
    20881 aattcgccat actacagaag ctcagggtaa tgtttttgaa gatcttggtt tccctattga
    20941 tgaagctcaa aaattgaaat ctgccagtca gcaactgatt gaaactaaac tgatgctaat
    21001 gaatgaaatg agcaactgga ttgataaaaa taatctcaaa caatcagaag cggcttctat
    21061 tctaggtgtt agccgccctc gaatttctga tatggtcaat ggcaagttag aaaagtttac
    21121 cctagatacc ttagtcactt ttgttgcaaa aaccggcaaa acaatccaat tgcaattatc
    21181 ctgatatttg caaaatttta ctcaaattca accgcttgtt gcttttatca ttcactaaca
    21241 aggttatttc aagtaaaatt gtgactaatt ttttattaat aaacacaaat tatggcaaaa
    21301 aaacctaaag ttgcatcaaa tacgattgcg ttaaataaac gtgccagaca cgaatatttc
    21361 attgaagatg aaattgaagc cggcttagag ctacagggct gggaagtcaa agcattgcgt
    21421 gccggaaaag gcaatatcag cgatagctat gtcatctttc gccacggcga agccttctta
    21481 ttcggggcaa tgattacgcc tttgaatatg gcatcaaccc atattgtcag cgacccgacc
    21541 cgcacccgca aacttctact gaataaacgt gagttagact cactgttcgg taaagttaat
    21601 cgtgaaggtt ataccattgt tgccacctca atgtattgga aaaatgcgtg ggtgaaagta
    21661 aaaatcggct tagccaaagg gaaaaaactg cacgataaac gtgaagatat taaagaccgt
    21721 gaatggcaag tggctaaaca gcgtattatg aaaaacgcca atcgtggcta aatgaataat
    21781 taccctctcc ccttgtggga gagggacaga aaaattgcgt tcagcaattt ttcagggaaa
    21841 gggggatttg taaaagttca aggaaaaaca acgcttgccc tctctctgaa aaatatttct
    21901 gaacgaaata tttttcggtc tctctcccat aaagggagag agtgaaacac aaatttaaac
    21961 taaggaacat acaatgatcg accaaaacct acttcgtacc aatttagaag acgttgctac
    22021 agctcttaaa atcaaacgta attttatttt agatgtagag cgagtaaaaa cccttgaaga
    22081 acagcgtaaa acgctacaag ttaaaaccga aaccttacaa gcagaacgca acactcgctc
    22141 caaaaacatc ggtgcggcaa aagctcgtgg cgaagatatt tcagctttat tagccgaagt
    22201 agattcaatg ggcaacgaat tagacacggc aaaagtagaa ttggataaag tacaggcaga
    22261 aatccgtgag ttgttgcttt ctgtgccaaa cttaccggca gatgaagtac cactcggtaa
    22321 agacgatagc gaaaacttag aagtattacg ttggggcgaa ccacgtcaat tcgactttga
    22381 agttcaagat cacgttaccc ttggcgaaaa cttaaacggt ttagattttg ctgccggtgt
    22441 aaaattaacc gccagccgtt ttgtggtaat gaaaggtaaa cttgcccgct tacaccgtgc
    22501 attatctcaa tttatgttgg atttacacac tgaacaacac ggctatgtgg aaaccaatgt
    22561 gccattttta gtcaatcacg acactctata cggcacaggt cagctaccaa aatttggcga
    22621 agatttattc catactcaac cattaacagg gcaagatcca aacgaggtgc aacgcccatt
    22681 cggtttgatt ccaaccgctg aagtgccagt aacgaactta gtgcgagatg aaatcattga
    22741 tgaagacagc ttaccactgc gttataccgc ccacacccct tgtttccgtg cagaagccgg
    22801 ttcttatggg cgtgatactc gtggtttaat tcgtatgcac caatttgaaa aagtggaaat
    22861 ggtgcaaatt gtcgcccctg aaaaatcaat ggaagcctta gaagaattaa ccggtcacgc
    22921 agaaaaagtc ttacagcttt taggcttgcc ataccgcaaa gtgctactct gcactggtga
    22981 tatgggcttt ggtgcagcaa aaacctacga cttagaagtt tggctaccgg cacaaaacac
    23041 ttaccgtgag atttcttctt gctcaaatat gtgggatttc caagcccgcc gaatgtctgc
    23101 acgttgtaaa gctaaaggcg acaagaaaac tcgcttggtt cacaccttaa acggctcagg
    23161 tttagcggtt ggtcgcacac tggtcgctgt gttagaaaac taccaaaatg ccgatggctc
    23221 tattactgtg cctgaggtgt taagacctta tatgggtggt ttggaagtaa ttaccgctta
    23281 agattaataa atgaaaccaa tctcagaaat ggggttggtt tttttaataa atcttatccc
    23341 taatcttttt caggataatt cataaaaatt tcagtaacta ctcggcggtt ttgcactgta
    23401 tctaaacgaa taggattttt cattctccct aaacgaaatg caataatggt gacttttttc
    23461 ggtt
//
LOCUS       DANLXF010000007        21930 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.007, whole genome shotgun sequence.
ACCESSION   DANLXF010000007 DANLXF010000000
VERSION     DANLXF010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 21930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21930
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.007"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(96..1700)
                     /locus_tag="PXD66_000169"
     CDS             complement(96..1700)
                     /locus_tag="PXD66_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDL6318509.1"
                     /translation="MALFLSVFPIVLLIYLMVKRNALPSYVALPLTALLIFVLQLTYF
                     GNDTTLIFANIIAGLGDVLTPITVIFGAILFNRFSEVSGATNTMRKWLGTINPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILVGLGFKPLQVAMLALVMNSVPVSFGAVGT
                     PTWFGMGPLLKDGLLTDAQVLEIGSITALIHSIAAFIIPVMALRLIVSWKEIRQNIVF
                     IYISIFACVIPYFIIAQFNYEFPSLVAGAIGLLVSVWVANMGIGLAKSENHLDGDKAT
                     FGEVAKALLPTGLLIFILVITRIQQLPLKAMLNDATAWIVSSLGFANFEISQGLIFAL
                     KNILGTNVATSYKLLYVPALIPFVVTVLICLPIFSVSGKNTQEIFGTSFKQVKKPFLA
                     LLGAIVMVKLMLIGGVDSMVQIIGKNFAEFTGQSWTLFASFLGAIGAFFSGSNTVSNL
                     TFGTVQYATAQQVGLSVTLILALQSVGGAMGNMVCINNIVAVCSVTGIEKQEGNIIKK
                     TAVPMFVYGVIAAISALFIVPMFYNL"
     gene            complement(1990..3081)
                     /gene="ychF"
                     /locus_tag="PXD66_000170"
     CDS             complement(1990..3081)
                     /gene="ychF"
                     /locus_tag="PXD66_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDL6318510.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANYPFCTIEPNTGVV
                     PMPDPRLDALAEIVKPERVLPTTMEFVDIAGLVAGASKGEGLGNKFLANIRETDAIGH
                     VVRCFENDDIVHVAGKIDPADDIEIINTELALADLDSCERAIQRLQKRAKGGDKDAKF
                     ELSIMEKILPVLENAGMIRSVDLDKDELHAIKGYNFLTLKPTMYIANVNEDGFENNPY
                     LDRVREIAEKEGAVVVPVCAAIESEIAELDDEEKVEFLQDLGIEEPGLNRVIRAGYRL
                     LNLQTYFTAGVKEVRAWTVSVGATAPKAAAVIHTDFEKGFIRAEVIAYQDFIDNKGEA
                     GAKEAGKWRLEGKDYIVQDGDVMHFRFNV"
     gene            complement(3267..3854)
                     /gene="pth"
                     /locus_tag="PXD66_000171"
     CDS             complement(3267..3854)
                     /gene="pth"
                     /locus_tag="PXD66_000171"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDL6318511.1"
                     /translation="MSEIKLIVGLANPGVKYEETRHNAGEWLINELARIYNVSLKEEP
                     KYFGKVAKINTAQGEVRLLVPTTFMNLSGKAVGALANFYRIKPEEILVAHDELDLPPG
                     SVKLKKGGGHGGHNGLKDIIAALGNNKEFYRVRIGIGHPGHKDLVANYVLGKPSPSDQ
                     TLLNSAVDEAARCVEVLFKDGMVKATNRLNSFKAN"
     gene            complement(3927..4802)
                     /locus_tag="PXD66_000172"
     CDS             complement(3927..4802)
                     /locus_tag="PXD66_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDL6318512.1"
                     /translation="MMKKFIPVMIAALVLSACSSQPAKRKSTPRIKATAAYIPTENSA
                     YVVFDLNQKKFLEGKNFNLVRPIASVTKLMTAVVFLEENRYGASCQTQILPSDADFLK
                     GTTSPLPKNLNIACSELLKAMLVRSDNYAAHALAHATHLNKAQFLARMNSKAREIGMT
                     QTYFGDSSGLSSDNVSTVVDLAKLAGYAASKPEIQQLSNSPLVSVYAGGRYFSMKNTN
                     SMVRSQSYQALVSKTGYTREAGYNLAFIAKGDCRGKRIGVVSLNNDNAASRTDFTESM
                     LAKYQCVPNPYRQYD"
     gene            5024..6604
                     /gene="prfC"
                     /locus_tag="PXD66_000173"
     CDS             5024..6604
                     /gene="prfC"
                     /locus_tag="PXD66_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDL6318513.1"
                     /translation="MSYPQEVNKRRTFAIISHPDAGKTTITEKVLLYGNAIQTAGSVK
                     GKGSAQHAKSDWMEMEKQRGISITTSVMQFPYNDCLVNLLDTPGHEDFSEDTYRTLTA
                     VDSCLMVIDSAKGVEERTIKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVESVLK
                     IKCAPITWPIGCGKLFKGVYHLYKDETYLYQTGQGHTIQEVRVVKGLNNPELDQAVGD
                     DLAQQLRDELELVQGASHEFDHTEFIKGELTPVFFGTALGNFGVNHFLDGLTEWAPAP
                     QARQSDEREVSPSEEKFSGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLKHVRIG
                     KDVVISDALTFMAGDRSQAEEAYAGDIIGLHNHGTIQIGDTFTQGENFKFTGIPNFAP
                     ELFRRIRLRDPLKQKQLLKGLVQLSEEGAVQVFRPLINNDLIVGAVGVLQFDVVVSRL
                     KTEYGVEAIYETVNVATARWVEGKDAKKFEEFKRKNEANLALDGGDNLTYIAPTMVNL
                     NLAQERYPDVQFFKTREH"
     gene            6863..8437
                     /locus_tag="PXD66_000174"
     CDS             6863..8437
                     /locus_tag="PXD66_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL6318514.1"
                     /translation="MKKWLIPLTVAAFCVSACDDKNSTQATAEKPQVENTQSQKNAEI
                     LPLATVVAPWEINSLDPNQSGVIFQRMNLAETLVEADLNGNLIPALATDWRSNEEGTI
                     WRFTLREGVKFHNDQVLTAQAVVNSLNIAKSKPGVLAKAFIKEIRAVDNKVEIELTKA
                     FVPFPPFLTHYTALILAPESYNEKGEVTQIIGTGAFKPTKIEAPQKLEAVQFEGYWGK
                     KPQVQQANYLASSRSETRMLMAQSEPSSLVFNLDTASVARLKADPNLQLVSGSIARTI
                     QLKMDVAKPFFNDLAIRQALSQAINRKAIAEQVLKIENGAADQILPKAFADWRVDTAE
                     QVVDIAKIKQNLTASGYTFDEQGRLTKDGKPFSFTMRTFSDRPELPVIATILQAQWKQ
                     IGVEVNVSVGNFSEIPAGHQDGSLEMALYALNYGKTLDPYGVIASDFANGGSDWGVMN
                     WQNTEVDNAVKSLETERDPQKAKALKQTVSKIIYNDLPIIPVVYYQQNVVAHKEVKNV
                     TLDPFERRFFLELLSK"
     gene            8657..9589
                     /locus_tag="PXD66_000175"
     CDS             8657..9589
                     /locus_tag="PXD66_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL6318515.1"
                     /translation="MHKILFRRLIQILLVIWSVGTLTFILTRQLSGDMAYRIAASRYG
                     YDQADSAAAEAVRAELGLDQPWWQSYFSWLSDLAQFNLGKSLVTGDPVWSEIQHQFGH
                     TLSLAIVALILAILIGPPLGLLAARKPNGFFDRLTLVFSTLFRSVPAFIIAIGLITLF
                     SAQLKWLPAGGYGCWQHFILPALTLAIGLSTVSVRVTRSAMVQVKGSEFYQFSQLKGL
                     SRWHTFLRHGLPNIAVPVIAYHAVQLVYLIEGVVVVESLFAWPGSGHALVHAIIARDV
                     PMIQGTTLVMGGLFVVLNTIADLLSAWIDPRIKR"
     gene            9599..10381
                     /locus_tag="PXD66_000176"
     CDS             9599..10381
                     /locus_tag="PXD66_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL6318516.1"
                     /translation="MKISISQYLGGTILLAMLGFAFLQPYFYPMDIGFQDLTNILSNP
                     NEIAWFGTDHLGRDMLARLASAIRLSFGLSLFSVICALIAGLITGIMAGFFGGWIDRI
                     FSFICDLVMALPGLLLVLLFAALSPGSFWTLYLGISLVMWVEFFRVIRSISSTMANSS
                     EIQASRLMGMGWFYSFKRHLLPRLLPMIATLSAFSLGNAILALATLGFVSVGLRPPTA
                     ELGLMMTELFPYYYEAPLIFLQPVVAVFLMVLSLQLLSRKIK"
     gene            10470..11882
                     /locus_tag="PXD66_000177"
     CDS             10470..11882
                     /locus_tag="PXD66_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL6318517.1"
                     /translation="MKLLDIQNLSVETENRLQLVEPISLQLKAGKNITILGETGSGKS
                     LLIQAIMGALPDGLIASGEISVKNCKISTESDRLQTLWGKTLVMLPQEPRRSLNPIMA
                     IGKQLWESFHYVAGKPSSQAKTESKAYLAKLGLGESVENYPHQLSGGMAQRASFAIAT
                     AAGGKILLADEPTKGLDPISKGKVIELLKSAYQNGGGLLTITHDIEVAEQLGGTILVM
                     KKGELLEQGEAKALLNNPQHPYTKALISADPKHWQPLQDLQNLSQKQPLVSVKNLSIA
                     RGKKTLFSNLSFDLHKGEVLGIVGHSGIGKSSLADVLCGLLKPKSGEVVWHSHSHKKH
                     QVLKLYQDPPEAFAPNVSLKTLLDDVINKHQLDRLPIPSLLQQLSLNPEILQRNAENV
                     SGGELQRVAILRALLLEPVLLFADEVTSRLDPITQQETIELLINQCRARNCSLILVSH
                     DPYLIEKSCDKVINLEEYVG"
     gene            complement(11969..12712)
                     /gene="rlmB"
                     /locus_tag="PXD66_000178"
     CDS             complement(11969..12712)
                     /gene="rlmB"
                     /locus_tag="PXD66_000178"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDL6318518.1"
                     /translation="MSEQIYGIHAVKAFLDNAPERLIEVLVLKGREDKRLMPLLNELQ
                     RLGVAIQQVNRQTLDNKSQGEVHQGIIARVVPQKELNEHDLEAILSQKKNPLLLILDG
                     VTDPHNLGACLRTADAAGVDAVIVPKDKSAQLTPTARKVACGAAEVMPLIRVTNLART
                     MRDLQERHNVWIVGTAGEAESGIYEAKLTGSIALVMGAEGDGMRRLTREHCDQLISIP
                     MAGSVSSLNVSVATGVCLFEIVRQKLAGQ"
     gene            complement(12767..14134)
                     /gene="pssA"
                     /locus_tag="PXD66_000179"
     CDS             complement(12767..14134)
                     /gene="pssA"
                     /locus_tag="PXD66_000179"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HDL6318519.1"
                     /translation="MLILNKQRKAEYLLKSLPYLPQVAEQVQFLASSKAFKQQILQLI
                     QTAKKRIYITALYFEKDEAGQEVLAALYQAKRNNPELEINILVDWHRAQRGRIGEAEN
                     LSNADWYAKVKYEQGLPAGQEIAFYGVSINGREVFGVLHLKGFVFDDTILYSGASINN
                     VYLHQFERYRYDRYHVIENKVLADSWVTFIQQHLLTNEAVKRLDTENRPKTAEIRAQI
                     KHFRKQLSREAYLFNGVAENGRLAVSPLVGLGRRKNQLNKVIEALFYQTEQKLTICTP
                     YFNFPHSLRTRIEWLLANGKSVEIIVGDKTANDFYTKPDEKFTMASALPYLYEKNLRA
                     FAKRLDGYVQNKQLTIRLWKDGENSYHLKGVWVDNRYILLTGNNLNPRAWRLDAENAI
                     LISDPQHQLSEKAETELNQICQHTQILTHYSDLEVLTDYPENVRKLLKKFGRVKLDKI
                     VKMLL"
     gene            complement(14204..15751)
                     /locus_tag="PXD66_000180"
     CDS             complement(14204..15751)
                     /locus_tag="PXD66_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HDL6318520.1"
                     /translation="MMKIIAFLFIFLSVFSHTALANSMAEQCVALKSNSPEKTAIYKA
                     EWVEKSAELPAYCLVQGEIGKRVEVDNVPYGIQFELRLPEQWNEKFLFQGAGGVGGVI
                     FPAIGKLYPHGASGANGLNRGYAVVSNDSGHATRDLVFTADPEARSNYAYASIGKVTT
                     VAKQLIAEFYRKPSKHNYIMGCSNGGREAMMAAMRYPQEFDGVIAGSPGFRVSRSVLA
                     EVWDNRALLAVAPKNGDGDKILSQALTQQDLDVIANGVLTRCDKLDGLADGLINAWEQ
                     CDFQPEMVAKQLGQKKVALIKTIFEGAKNSRGELIYSPWAYDSGINAKGWRDWKLGDS
                     QTAQPNSISFKMGLKSLTHYYLAPRVPSRDPLSVDLDQASEQVKAVGSIHDTDQVDLS
                     AFKQNGGKMLIYQGVSDPIFSAVDLKNWYQSLQQAVENPQHFAKLFFVSAMNHCGRGA
                     TVNDFDMLTALENWVEKGLEPESIAAKAGELYPNKAKQIPLCAYPKVAIYQTGEDPNR
                     LTSYQCR"
     gene            complement(15876..18692)
                     /gene="ileS"
                     /locus_tag="PXD66_000181"
     CDS             complement(15876..18692)
                     /gene="ileS"
                     /locus_tag="PXD66_000181"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDL6318521.1"
                     /translation="MTIDYKNTLNLPETGFPMRGDLAKREPDMLKNWYDKNLYQKIRT
                     ASKGKKTFILHDGPPYANGNIHLGHAVNKILKDMILKSKTALGFDTPYVPGWDCHGLP
                     IELKVEGIVGKPNEKISAAEFRQACRDYAKEQVEGQKADFIRMGILGDWDNPYLTMNF
                     NTEANIIRTLGKVIANGHLYKGSKPVHWCLDCGSSLAEAEVEYEDKVSPSIYVRFNAV
                     DVNAVEEKFNAQGKGRGALSAVIWTTTPWTIPSNRAIAINPELDYALVQLGDERVLLA
                     VDLVEDVAKAAGVESAEILATTKGENLELLRFNHPFYDYSVPFIFGDHVTTDGGTGLV
                     HTAPDHGADDFVVARKYNIEMAGLISNDGKFKADTPFFAGLGVFESNDKVVEKLQEVG
                     ALLKLSRIKHSYPHCWRHKTPIIFRATPQWFIGMETQGLRQQALGEIKSVRWIPSWGE
                     ARIDTMVANRPDWCISRQRTWGVPMAMFVHNETEELHPRTLELIEEVAKRVEEKGIQA
                     WWDLDPAELLGEEAKDYRKVPDTLDVWFDSGSTYASVVEQRPEFNGNSADMYLEGSDQ
                     HRGWFMSSLMLSTATNGKAPYKQVLTHGFTVDEKGRKMSKSLGNVIVPSEVWNKNGAD
                     ILRLWVASTDYTGEITVSHNILNSAGESYRRIRNTARFLLANLNGFDPKSDLVQPQEM
                     IALDRWAVACALEAQNDIKEAYDNYQFHTVVQRLMRFCSIEMGSFYLDIIKDRQYTTK
                     ADSLARRSCQTALWHIAEALVRWIAPILSFTADEIWGYLPQVEGRSEFVFTEEFYTGL
                     FGLTEQDKLDDAYWQQILKVRAEVNRVLEQARKDKVIGAGLEAKVTVFANDEIRPLLE
                     QLGNELRFVLITSQAIVKPLAEADIAEGELAGLAVKVENADGEKCPRCWHYATDIGSH
                     SGHEEVCGRCVENVAGEGEKRLFA"
     gene            complement(18795..19757)
                     /gene="ribF"
                     /locus_tag="PXD66_000182"
     CDS             complement(18795..19757)
                     /gene="ribF"
                     /locus_tag="PXD66_000182"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HDL6318522.1"
                     /translation="MQLIRGFHNLDKHIALNQGCVLSIGNFDGVHLGHQNILARLCDR
                     ALDLGLPSVVMLFEPQPREFFAKKSGNSTACNPPARLMRLRDKLKYLEEAGVDFVLCV
                     RFSEKFAQISADDFISDLLAKRLKVRYLSVGDDFRFGAKRAGSFETLRNAGLKYRFAV
                     EESHTHSLNEERISSSLVREALQNNELDLAEKLLGKPYSILGRVAHGNKLGRTIGFPT
                     ANIMLNRLVTPIQGVFAVKVKTKQGSFNGIANVGNRPTINGTKPLLEVHIFDFNASIY
                     GEAIEVIFLKKLRNEIKFPDFEALKAQIEKDRLQAVEFFKEFAR"
     gene            19981..21255
                     /gene="nadR"
                     /locus_tag="PXD66_000183"
     CDS             19981..21255
                     /gene="nadR"
                     /locus_tag="PXD66_000183"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HDL6318523.1"
                     /translation="MTDFAYLQQKRKQLKLKVNDICEQAGVTRAYFNQLVSGKIKNPS
                     ANKLKALHSVLDIVEDSNQRSGVIFGKFYPIHTGHINMIYEAFSKVDVLHVIVCTDSE
                     RDLRLFQESKMKRMPTNEDRLRWVQQIFKYQQKQIFIHHLVEDGIPSYPNGWEDWSGR
                     VKALFEEKSINPTIVFSSEIQDKESYEKYLNLEVQLVDPTRESFNISATQIRNNPFQY
                     WRFIPKEVRPFFVKTIAILGGESSGKSVLVSKLANVFNTTSAWEYGREFVFEQLGGNE
                     QAMQYSDYPQMVLGHQRYVDYAMKHAHKVAIIDTDYITTQAFCIQYEGKAHPFLDSMI
                     REYPFDVTILLSNNTKWVADGLRSLGSKKQRQQFQHLLKKLLEKHHIPYIKIESPSYL
                     DRYNQAKEVVEAILNEEPIPLQFKQYNIHEFE"
     gene            21273..>21930
                     /locus_tag="PXD66_000184"
     CDS             21273..>21930
                     /locus_tag="PXD66_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL6318524.1"
                     /translation="MILFAGDPHGSYEHLYPILQQQTEEVALVILGDLQLTTPDELDR
                     LSQYCDIWFIHGNHDSKTVAAFDAIWNSSWKERNIHGKVVDIQGIKIAGIGGVFRGHI
                     WMPPNRPMFFDPIHFCQYSAPEKIWRGGIPLRHRSSIFPSDFEALENEKADILITHEA
                     PKPHPQGFSVINQLARKLGASKIFHGHYHDNFNYGNLNVSRECAIYNIGFRSLADING
                     N"
BASE COUNT         6285 a         4957 c         4404 g         6284 t
ORIGIN      
        1 cgttctaaga cggcgaaagg aagaggtgga tttgcaaaaa atttgctaaa tctcaccgct
       61 tcctttctaa atcaagtaag ctgattatta actcattata ggttatagaa cataggtact
      121 ataaacaatg ccgaaatcgc agcaataacg ccatacacga acattggcac ggctgttttc
      181 ttaataatat tgccttcttg tttctcaatg ccggtaacag aacacacagc cacgatgttg
      241 ttaatacaca ccatattccc cattgcacca ccaactgatt gcaatgcaag aattaaggta
      301 acagataacc cgacttgctg tgccgttgca tattgtactg ttccgaaggt taagttagat
      361 accgtatttg accctgagaa gaacgcacca attgcaccta agaacgaggc aaataatgtc
      421 caagattgac cggtaaattc tgcgaagttt ttaccaatga tctgaaccat tgagtctaca
      481 ccaccaatta acattaattt taccatcaca atggcaccta aaagtgctaa gaatggtttt
      541 ttcacttgtt taaatgaagt gccaaaaatc tcttgagtat ttttacctga taccgagaaa
      601 atcggtaagc aaattaatac ggtcacaaca aatggaatca acgcaggaac atacagtaat
      661 ttatagctag ttgctacatt cgtacctaaa atatttttca gtgcaaaaat taagccttgg
      721 ctaatttcaa agttagcgaa accaagcgaa ctgacaatcc aagctgttgc atcatttagc
      781 attgctttta atggaagttg ctgaatacgg gtgattacca aaatgaagat cagtaaccct
      841 gttggtaata atgcttttgc cacttcacca aaggtcgctt tatcgccgtc aaggtggttc
      901 tcgcttttcg ctaaaccaat acccatattt gctacccaga ctgacaccaa taaaccaatc
      961 gccccggcaa ctaatgatgg gaattcgtag ttaaattgag caataatgaa gtaaggaatg
     1021 acacaggcaa aaatgctgat ataaataaac acgatatttt gacgaatttc tttccagctc
     1081 acaattaaac gcaatgccat aactggaata ataaacgcag caatagagtg gatcagtgca
     1141 gtaatagaac caatttcaag cacttgtgca tcggttagta aaccatcttt taataatggt
     1201 cccataccaa accaagttgg tgtgccaacc gcaccaaatg acaccggcac agagttcatt
     1261 actaaggcta acattgccac ttgtaacggt ttaaagccta aaccaaccag aattggagct
     1321 gcaattgccg ccggcgtacc gaaacctgat gcgccttcaa tcataaatgc aaatgcccaa
     1381 ccgataatca tcaactgtgc caccggattc gggttaattg tccctaacca tttacgcata
     1441 gtgttggttg cacctgaaac ttcagagaaa cggttaaata aaatcgcccc gaaaataacg
     1501 gtaattgggg ttaatacatc acctaaaccg gcaataatat tggcaaagat taacgtggta
     1561 tcattaccaa aataggtaag ctgaagcaca aaaattaata acgccgtaag tggtaaagcc
     1621 acataagacg gtaacgcgtt acgttttacc attaaataaa tgagcaaaac aattggaaat
     1681 acgctcagga atagagccat actgcacctc tttatatgat tataaaatga cctaactggt
     1741 cggataggtg cgaattgtac tcttaaaaaa aactaatgta actaggttgt tacaattttg
     1801 tgatctagct cgcattttta atgattgtat ggttaaaaaa gagccatatt tattttcatt
     1861 taatacttaa tagttagccc tatttaagac atttttaccg aatcgagcaa tatgcaatta
     1921 attaacaaaa aaaggccgct tgtagatcac agatcaacaa gcggtctttt ttaggcaaaa
     1981 atttgcaaat taaacattga aacggaagtg cattacatcg ccgtcttgaa caatataatc
     2041 tttaccttcc aaacgccatt tacccgcttc ttttgcaccg gcttcacctt tattgtctat
     2101 gaagtcttgg taagcaatta cttccgcacg aataaagcct ttctcaaagt cagtgtgaat
     2161 cacggctgct gcttttggag cggttgcacc taccgaaacc gtccacgcac gcacttcttt
     2221 tacccctgcg gtaaagtagg tttgtaagtt aagtaaacgg tagcctgcac gaataacacg
     2281 gtttaagccc ggctcttcaa tgcctaaatc ttgtagaaat tccacttttt cttcgtcatc
     2341 aagctcggca atttccgatt caatcgctgc acacaccggt acaaccaccg ccccttcttt
     2401 ttcggcaatt tcacgcacac ggtcaagata agggttattt tcaaaaccgt cttcgttcac
     2461 gttggcaata tacattgtcg gttttaaagt taagaagttg tagcccttaa tagcgtgcag
     2521 ttcatcctta tctaaatcta ccgaacgaat cataccggca ttttcaagca caggtaggat
     2581 tttttccata atcgataatt caaattttgc gtctttatcg ccacctttcg cacgtttttg
     2641 taaacgctga atggcacgct cgcaactgtc taaatcggca agtgccaatt cggtattgat
     2701 gatttcaata tcatcagctg ggtcaatttt ccccgcaacg tgaacgatgt catcattttc
     2761 aaaacaacgc acaacgtggc caatcgcatc agtttcacga atattggcta agaatttatt
     2821 acctaaacct tcgcctttgc ttgcacctgc cactaagccg gcaatatcca caaattccat
     2881 tgtagttggt aacactcgct caggtttcac aatttccgcc aacgcatcta agcgagggtc
     2941 aggcattggt accacaccgg tatttggctc aatcgtacag aacggatagt ttgctgcctc
     3001 aatacctgct ttagttaatg cattgaaaag ggtcgattta ccgacatttg gtaaacccac
     3061 aataccacat ttaaatccca ttttttgttc cttagaattc ccctctcccc ttgcgggaga
     3121 gggataaatt tgagcttcgt tcagaagcga aaatgaggga gagggcgaaa ttgcaataat
     3181 ttcctcaaat atgaccgctc ccctctctct gaaaaatact tctgaacgaa gtatttttcg
     3241 gtctctctcc cacaagggag agagttttaa tttgctttaa aactatttaa acggttagtt
     3301 gccttaacca ttccgtcttt aaataaaact tctacgcaac gtgcggcttc atcaaccgca
     3361 ctattcagta atgtctgatc gcttggtgaa ggcttgccca gcacataatt cgccactaag
     3421 tctttatgcc ccgggtgtcc aataccaatt cgcacacggt aaaactcttt attattgcca
     3481 agggcggcaa taatatcttt caagccgtta tgaccaccgt gtccgccgcc ttttttcaat
     3541 ttcaccgaac cgggtggtaa atccaattca tcgtgagcga ccaaaatttc ttccggttta
     3601 atccgataaa aattggctaa cgctcccact gctttaccac ttaaattcat aaaagtagtc
     3661 ggcactaata aacggacttc gccttgagcg gtattaattt ttgctacttt gccaaaatat
     3721 ttcggctctt cttttaggct aacgttatat attcgggcaa gttcgttaat cagccactcg
     3781 cctgcattat ggcgggtttc ttcatatttt acccccgggt tcgccagccc gacaattaat
     3841 ttaatttctg acacaatctt gtctcaattc atttaattta aaaggcagta ttttagagaa
     3901 aaaccgctat tttctcaagg caaccattaa tcatattgtc tatacggatt tggtacgcat
     3961 tgatacttcg ctaacatcga ttcagtgaaa tcggtgcgtg aagcagcgtt atcattgttt
     4021 aaactaacca caccaattcg cttgcctctg caatcccctt tagcaataaa ggcaagatta
     4081 taacccgcct cacgggtata acctgtctta ctcactaatg cctgatagga ttgggaacga
     4141 accatactgt tggtgttttt catagagaaa taacgaccac ctgcataaac actcactaat
     4201 ggcgaattag acaattgctg aatttcaggc ttacttgccg catagcctgc caatttagca
     4261 agatcaacca ccgttgaaac attatccgaa gataagcctg agctatcacc aaagtaggtt
     4321 tgagtcatac cgatttcacg ggctttgctg ttcattcgag ccaaaaattg ggctttattc
     4381 aaatgtgttg cgtgggcaag ggcgtgagcg gcatagttat cggagcgaac tagcatcgct
     4441 tttaacaatt cactacaggc aatgttaaga tttttcggca atgggctggt cgtgcctttt
     4501 aagaaatcag catctgacgg caaaatttgg gtttggcaac tcgccccata gcggttttct
     4561 tctaagaaaa cgactgctgt cattaatttt gtgaccgaag caatcgggcg aactaaattg
     4621 aaattcttac cttccaaaaa tttcttttgg tttaagtcaa acaccacata agcggaattt
     4681 tcggttggaa tataagcagc ggttgcttta atccgtggtg tgcttttcct ttttgccggc
     4741 tgggaggaac aggcgcttaa caccagagcc gcaatcataa caggaataaa ttttttcatc
     4801 ataatagatt catcgataat aaagtcaaag ctggttattc taccctgttt ccaaaattgt
     4861 gccaaagcaa gcggtcgatt tttacaaaat ttttgcaaat ctagccgatt ttgcaaaatt
     4921 tccgccaaat ttaaccgctt gccctctcgc ctcaaatcag cgaaaataac gcttaatttt
     4981 ttggcggact ttccgccctt tttattttaa ttggttgaga aaaatgtcat acccacaaga
     5041 agttaataaa cgtcgcacct ttgcgattat ttcccacccc gatgcgggta aaaccaccat
     5101 taccgaaaaa gtattgctct acggaaatgc aatccaaact gccggctcgg taaaaggcaa
     5161 aggctcggca caacacgcta aatccgactg gatggagatg gaaaaacaac gtggaatttc
     5221 gattaccacc tccgtgatgc agttcccgta caatgattgc ttggtaaact tactcgatac
     5281 accggggcac gaagacttct cggaagatac ttatcgtact ttgactgcgg tagatagctg
     5341 cttaatggtt atcgactcgg caaaaggggt tgaggaacgc accattaaat taatggaagt
     5401 gacccgcttg cgtgatacgc caatcctaac ctttatgaac aagctagacc gtgatattcg
     5461 tgacccgatg gagctgttag atgaagtgga aagcgtgctg aaaatcaaat gtgcgccgat
     5521 cacttggccg attggttgcg gaaaattgtt taaaggggta tatcaccttt acaaggacga
     5581 aacctactta taccaaaccg gacaaggtca taccattcaa gaagtacgag tggttaaagg
     5641 cttaaataat ccggaactgg atcaagccgt tggcgatgat ttagcccagc agttgcgtga
     5701 tgaattagag ctggtacaag gcgcttcgca cgaattcgat cacaccgaat ttatcaaagg
     5761 cgagctgaca ccggttttct ttggcactgc actgggtaac ttcggggtca atcatttcct
     5821 tgacggttta accgaatggg caccggcacc acaagcccgt caatcggacg aacgtgaagt
     5881 ttcaccaagc gaggaaaaat tcagcggttt cgtatttaaa attcaagcga atatggatcc
     5941 gaaacaccga gatcgagttg cttttatgcg tgtcgtttca ggtaaatatg aaaaaggaat
     6001 gaagcttaaa cacgtccgta tcggcaaaga tgtggtgatc tccgatgcct taacctttat
     6061 ggcgggcgac cgttcacaag ccgaagaagc ctacgccggc gatattatcg gcttgcacaa
     6121 tcacggcacg atccaaattg gtgatacttt cacccaaggc gaaaatttca aattcaccgg
     6181 tattccgaac ttcgcccctg agctgttccg ccgaatccgc ctgcgtgatc cgctcaaaca
     6241 gaaacaactg ctcaaaggct tggtacaact ttccgaagag ggtgcggtgc aagtattccg
     6301 tccgttaatc aacaacgatt tgatcgtggg agcggttggg gtgctacagt ttgatgtggt
     6361 ggtttcacgg ctgaaaaccg aatacggtgt agaggcaatt tatgaaaccg taaacgttgc
     6421 cacagcccgt tgggtcgaag gcaaagatgc caaaaaattt gaagagttca aacgcaaaaa
     6481 cgaggcaaac cttgccctag acggcggaga caacctcacc tacattgctc cgacaatggt
     6541 gaacttaaac ctcgcccaag aacgttaccc tgacgtacag ttctttaaaa cgagagagca
     6601 ctaaaaccaa taggggcgaa aaatctttcg cccctacaaa taaagcacaa gcggtcaatt
     6661 ttttttccga attttacaat actaggcatt ctgttcctca aatagcctaa atattcccct
     6721 atcaacaatc cttatcttga gattattctg cctattaatt aaccactcaa aaataccctc
     6781 gcagaaaact ctactttttt tatggaaata tgctaaagtt cgccaatatt catagccctg
     6841 atttttttat ggagaaaata aaatgaaaaa atggttgatt ccattaactg tcgcagcgtt
     6901 ctgtgtttct gcttgcgatg ataagaacag cactcaagca accgcagaaa aaccgcaagt
     6961 agagaatact caatcgcaaa aaaatgcgga aatattaccg cttgcaaccg tggttgcccc
     7021 atgggaaatc aatagccttg acccgaatca atcaggcgta attttccagc gtatgaactt
     7081 agcggaaaca ttggtcgaag ccgatcttaa cgggaattta attccggcat tggccactga
     7141 ctggcgcagt aacgaagagg gaacaatctg gcgttttacc ttgcgtgaag gggtaaaatt
     7201 ccataacgat caagtactta ccgcacaagc agtggtaaat agtttgaata tcgcaaaaag
     7261 caaaccgggt gtgttagcaa aagcctttat caaggaaatt cgagcggtag ataacaaagt
     7321 ggaaattgag ctaactaaag cttttgtgcc gtttcctccc tttttaacac actacaccgc
     7381 cttaattttg gcacctgaaa gctataatga aaaaggcgaa gtgacccaaa ttatcggtac
     7441 aggtgcgttt aaacccacaa aaattgaagc tccgcagaaa ttggaagcgg tgcaatttga
     7501 gggctattgg ggcaaaaaac cgcaagttca gcaagccaat tatttagcca gcagccgtag
     7561 cgaaacccgt atgttaatgg cacaaagcga gccgtcatca ctggtattta atttagatac
     7621 cgccagtgta gcaaggctga aagccgatcc taacctgcaa ttagtcagcg gatctattgc
     7681 ccgtactatt cagttgaaaa tggacgtggc aaagccgttt tttaacgact tagcgattcg
     7741 ccaagcctta tcgcaagcga ttaatcgcaa ggcgattgct gagcaggtct taaaaattga
     7801 gaacggagct gccgatcaaa ttttaccgaa agcctttgcc gactggcgtg ttgataccgc
     7861 agaacaagtg gtcgatattg caaaaattaa acaaaatctg accgcttccg gctatacctt
     7921 tgatgaacaa ggtcgcttaa ccaaagacgg caaaccgttt agctttacca tgcgcacctt
     7981 ctcagatcgt cctgaattac cggtgattgc tactatctta caagctcaat ggaaacaaat
     8041 tggcgtggaa gtgaatgttt cggtgggtaa tttcagcgaa attccggcgg ggcatcaaga
     8101 cggcagttta gaaatggctc tttatgcctt aaattatggc aaaaccttag atccatatgg
     8161 cgtaattgcc tccgacttcg ccaatggtgg cagtgattgg ggggtaatga attggcaaaa
     8221 tactgaggta gacaatgcag tcaaatcgct cgaaaccgag cgtgacccgc aaaaagcgaa
     8281 agcattaaag caaacggtta gcaaaattat ttacaatgat ttgccaatca ttccggtggt
     8341 ttattaccaa caaaatgtgg tggcacataa agaagtaaaa aatgtgacgc tagatccatt
     8401 tgaaagacgc ttctttttgg agttattaag taagtagttt aattcaaaat actcctcgct
     8461 ttagaaaagg gaaggtagga tttttcagta gtaatttgcg ctcttaggtg aataaatagt
     8521 gacagaatta tcgttttttc tcatttttca cctattttct ccgcttattt cttctgccaa
     8581 atcttccctt cccctctttg ctaaagaggg ggatcagaac gcataaaatc atctttttaa
     8641 taaatgaaat aatacaatgc acaaaattct cttccgccgt cttatccaaa ttctactggt
     8701 gatttggtcg gttggcacac tcacatttat cctcacccgc caactttcgg gtgatatggc
     8761 ataccgcatt gctgccagcc gctatggtta tgatcaagca gatagtgctg ctgctgaggc
     8821 tgtgcgtgcc gagctgggat tagaccaacc ttggtggcaa agctacttca gctggctaag
     8881 tgacttggct caatttaatt taggcaaatc attggtgacc ggtgatccgg tctggtctga
     8941 aattcagcac caattcgggc ataccttaag ccttgcgatt gtggcactta tcttagcaat
     9001 ccttatcggg cctccgctcg gtttgttagc cgcccgtaag ccaaacggct tttttgaccg
     9061 cttgacactg gtattcagta ccctgtttcg ctccgtgccg gcatttatta ttgcgattgg
     9121 cttgattacg ctgttttccg ctcaactaaa atggctacct gccggcggat acggctgttg
     9181 gcaacatttt atcttgcctg ccttaacgct tgcgattggc ttgagtacgg tttcggtacg
     9241 agtcacccgt tctgcaatgg ttcaggtaaa aggctcagaa ttttaccaat tttcacaatt
     9301 aaaaggctta agccgttggc ataccttttt acgtcacggt ttgccgaata ttgccgtgcc
     9361 ggtcattgcc taccacgcag tacaattagt ttatttaatt gaaggcgttg tggtggtcga
     9421 aagcctattt gcttggcccg gcagcggaca cgcactggtt cacgccatca ttgcccgtga
     9481 tgtgccgatg attcaaggta caacgctcgt gatgggcgga ttgtttgttg tattaaacac
     9541 cattgccgat ctattaagtg cttggattga cccacgaatt aagcgatagg acatcaagat
     9601 gaaaatttca atttcacaat atttaggcgg cactattctc cttgcaatgt tgggatttgc
     9661 ctttttacag ccttattttt acccgatgga tattggcttt caagatttaa ccaatatcct
     9721 ttccaatcca aatgaaatcg cttggttcgg caccgatcat ctcgggcggg atatgcttgc
     9781 ccgtcttgcc tctgctattc gtctttcgtt tggcttatca ctgtttagcg tgatttgtgc
     9841 cttaattgca ggcttaatta ccggcattat ggcaggcttt ttcggtggct ggattgaccg
     9901 cattttcagc tttatttgtg atctagtgat ggcactcccc ggcttacttt tagtattgct
     9961 ctttgccgcc ctttctcccg gctcattttg gacgctctat ttagggattt cattggtaat
    10021 gtgggtggaa tttttccgtg tgatccgctc tatcagttca acaatggcaa acagctcgga
    10081 aattcaagcc tcacgcctaa tgggaatggg ctggttttat agcttcaaac gccacttatt
    10141 gccccgttta ttaccgatga ttgccacttt atcggcattc tcgctcggca atgcgatttt
    10201 agctctcgcc acacttggct ttgtcagcgt agggcttcgc ccgcctacag cagaattagg
    10261 cttgatgatg accgaactat tcccttacta ctacgaagca ccgctgatat ttttgcaacc
    10321 agtggttgcc gttttcttaa tggtactcag cttacaatta ttgtctagaa aaattaaata
    10381 atgcgtaggg gcaggtcctg tgcctgccca aaattcttat tatatttatg ggcgggcaca
    10441 gggcccgccc ctacagtaat aaaatgaaga tgaaattact cgatatccaa aacctatctg
    10501 ttgaaaccga aaacagatta cagctggtcg aaccgattag cctacaatta aaggcaggaa
    10561 aaaatattac tatcttaggc gaaaccggct caggcaaaag cctgctgatt caagcgatta
    10621 tgggggcgtt acccgacggc ttaatcgcaa gcggtgaaat ttcggtaaaa aattgcaaaa
    10681 tttccaccga atccgaccgc ttgcaaacat tgtggggtaa aaccttggta atgctgccac
    10741 aagagcctcg ccgctcgctt aatccgatta tggcaatcgg caagcagttg tgggaaagtt
    10801 ttcactatgt ggcaggcaaa ccatcatctc aagccaaaac ggaaagcaaa gcctatttag
    10861 ccaaacttgg gctgggcgaa tcggttgaaa actacccaca ccagctttcc ggtggaatgg
    10921 ctcaacgtgc ctcttttgcg attgcaactg ccgccggtgg caaaatttta cttgccgatg
    10981 agccgaccaa agggcttgac ccaatcagta aaggcaaagt gattgaactg ttaaaatccg
    11041 cctaccaaaa cggtggaggc ttgctcacca ttactcacga tattgaagtt gccgagcaac
    11101 tcggtggcac tattttagtg atgaaaaaag gcgaactgtt ggagcaaggt gaggcaaaag
    11161 cgttgttaaa taacccacaa cacccttaca ccaaagcact tatcagtgcc gatccaaaac
    11221 attggcaacc attgcaagat ctgcaaaatt taagccaaaa acaaccgctt gtttcggtta
    11281 aaaatttgag tattgcccgt ggtaagaaaa cgctgtttag caacctttcc tttgacttac
    11341 acaaaggcga agtgcttggg attgtcgggc atagtgggat tggcaaaagc tcgcttgccg
    11401 atgtactttg tggtttgctt aagccgaaat cgggcgaggt tgtgtggcac agccacagcc
    11461 ataaaaaaca ccaagtgttg aagctttacc aagacccacc ggaagccttt gctccgaatg
    11521 tcagtctaaa aaccttgctt gatgatgtga ttaacaagca ccaattagat cgactgccga
    11581 ttccaagttt actgcaacaa ctttcgctca acccagaaat tttgcaacgc aatgcggaaa
    11641 atgtctccgg cggcgagcta caacgagtag caattttgcg agccttgctc ttagaaccgg
    11701 tactactgtt tgccgatgaa gtcacttctc ggcttgaccc aatcacacaa caagaaacga
    11761 tagaattgct gatcaaccag tgccgagcga gaaattgcag tctgattttg gtcagtcacg
    11821 atccttatct aattgagaaa tcgtgcgata aagtgattaa tttggaagag tatgtgggct
    11881 aacaagcggt tcaatttctg agattttttg cggctctaaa aacaaaaatg cgaagacatc
    11941 agttcttcgc atttttctta cttattcctt attgcccagc cagcttctgc cgtacaattt
    12001 caaacagaca gaccccggtt gcaactgaga cattcaaaga agatactgag cccgccattg
    12061 gaatgctgat aagctggtcg caatgctcac gggtaaggcg acgcattccg tcgccttctg
    12121 cccccataac taacgcaatc gagccggtta attttgcttc ataaatgccc gattccgcct
    12181 cacctgccgt gccgacaatc cagacattat gccgttcttg taaatctcgc attgtgcgag
    12241 ccagattggt aactcgaatc agtggcatca cttccgctgc cccacaggcg actttgcgag
    12301 cggttggtgt aagctgagca gatttatctt tcggcacaat aaccgcatca acccctgccg
    12361 catcagcggt acgcaaacaa gcccctaggt tgtgagggtc ggtcacgcca tctaaaatca
    12421 acaagagtgg atttttcttt tggcttaaga tcgcctctaa atcgtgttca ttcagctctt
    12481 tttgtggcac gactctggca ataatccctt gatgcacttc accttgcgat ttgttatcta
    12541 aggtttggcg gttcacttgt tgaattgcca cccctaaacg ttgtaattca tttaatagtg
    12601 gcattaagcg tttatcttca cgccctttta gcaccagcac ttcaattaaa cgctcaggtg
    12661 cattatccaa aaaggctttc actgcgtgaa tgccataaat ttgttcgctc atattttatc
    12721 cttaataatt gggcgaaccc atcagctcgc ccatataaat acctaactaa agcaacatct
    12781 tcacaatttt atccaactta accctgccga attttttcag caatttacgc acattttcag
    12841 ggtaatcggt taaaacttcc aaatcactgt agtgagttaa aatctgcgta tgttggcaaa
    12901 tttggtttaa ctccgtttct gctttttcac ttaattgatg ttgaggatcg gaaattaaaa
    12961 ttgcattttc ggcatctaac cgccacgctc ttgggttaag gttattgccg gtcagcaaaa
    13021 tatagcggtt atccacccaa acccctttta agtggtagct gttttcgcca tctttccata
    13081 agcgaatggt taactgttta ttttgtacat aaccgtccaa ccgttttgca aaggctcgta
    13141 agttcttttc atacaaataa ggcagagccg atgccatcgt gaatttttcg tccggcttgg
    13201 tgtagaaatc attagcggtt ttatccccca caataatttc aaccgatttg ccgtttgcca
    13261 atagccattc aatacgggtt ctcagagaat gcggaaaatt aaaatacgga gtgcaaatcg
    13321 ttaatttctg ctcagtttga taaaacaacg cttcaatcac tttatttaac tgattcttac
    13381 gtcgccctaa gcctactaaa ggagaaaccg ctaagcgacc attttcagca acaccgttaa
    13441 ataaatacgc ttcccgactt aattgtttgc ggaaatgctt aatttgcgca cgaatttcag
    13501 ccgttttcgg gcgattttcc gtatctaaac gttttaccgc ttcattggtt aaaagatgct
    13561 gttgaataaa cgtcacccag ctatcggcaa gaactttatt ttcaatgacg tggtaacggt
    13621 cataacggta acgttcaaat tgatgcaaat acacattatt aatgcttgct ccgctataca
    13681 aaatcgtatc gtcaaatacg aagcctttca aatgcaatac accaaacact tctcgtccgt
    13741 taatcgacac accgtaaaag gcaatctctt gccctgccgg taaaccttgt tcgtatttaa
    13801 cttttgcata ccagtcggcg ttagataaat tctctgcctc accaatacgc ccacgttggg
    13861 ctcggtgcca atcaaccaaa atgttaattt ccagttcagg attatttcgc ttcgcttgat
    13921 ataaagcagc taatacttcc tgcccggctt catctttttc aaagtaaaga gcggttatat
    13981 aaatacgctt ttttgccgtt tgaatcagct gtaaaatttg ttgtttaaac gctttgctcg
    14041 aggcgagaaa ctgaacctgc tctgccactt gtggtaagta tggcaggctt ttcaataggt
    14101 attcagcttt acgttgtttg ttcaaaatta acatcattga ttccaaaatt ttcaatcgtt
    14161 gcctgctatt ttacactgaa atcgccaata aagcgatttt cgcttaacgg cattgatagc
    14221 tggtcaaacg attaggatct tctccggttt gataaattgc gactttcgga taagcacaaa
    14281 gcgggatctg cttggcttta ttagggtaaa gctcaccggc tttggcggca atactttccg
    14341 gctccagtcc tttctcaacc caattttcaa gggcagtgag catatcaaaa tcattcaccg
    14401 ttgcccctcg cccacaatgg ttcattgccg acacgaaaaa caattttgca aaatgttgcg
    14461 gattttcgac cgcttgttgt aggctttgat accaattttt gagatcaact gccgaaaaaa
    14521 tcgggtctga aacgccttga taaatcagca ttttgccacc gttctgctta aaagcggata
    14581 aatcaacttg atctgtatcg tgaatgctcc ctaccgcttt cacttgttcg ctggcttgat
    14641 ccaaatctac gctgagtgga tcacggctcg gcactctggg ggctaaatag taatgggtca
    14701 aacttttcaa tcccatttta aaactaatgc tgttgggctg agcggtttgg ctatcaccta
    14761 atttccaatc ccgccagcct ttagcgttaa tgccggaatc atacgcccaa gggctataaa
    14821 tcagctcgcc tcgactgttt ttcgccccct cgaaaatcgt tttgattaaa gcgacttttt
    14881 tctgccccag ttgctttgcc accatttccg gctggaaatc acactgttcc cacgcattaa
    14941 ttaaaccgtc tgctaagccg tctaacttat cgcaacgtgt taatacgcca ttggcaatca
    15001 catctaaatc ttgttgagtg agggcttggg ataaaatttt gtcgccatca ccattcttcg
    15061 gagcaactgc caacaaggct cgattatccc atacttccgc caagactgaa cgggaaaccc
    15121 gaaagccggg gctgcctgca atcacaccgt caaattcctg tggataacgc attgccgcca
    15181 tcatcgcctc acgaccacca ttcgagcagc ccataatgta attgtgcttg ctcggttttc
    15241 ggtaaaattc ggcaatcaac tgcttggcaa ccgtggtcac tttgccgata gaggcatagg
    15301 cataatttga gcgagcttcg ggatctgcgg taaacactaa atctcgagtg gcgtgaccac
    15361 tgtcgttact cactactgca tagcctctgt ttaagccatt tgcaccactc gccccgtgcg
    15421 gatagagctt accaatggca gggaaaatca ccccacctac accgcccgcc ccctgaaata
    15481 agaatttttc attccattgt tcgggtaaac gtagctcaaa ttgaataccg taaggaacat
    15541 tatccacctc cacccgtttt ccaatttcac cttgtactaa acagtaagcc ggcaactcag
    15601 cagatttctc aacccattca gctttataaa tagccgtttt ttccggcgag ttagatttta
    15661 acgcgacaca ttgttcagcc atagaattag ccaaagcagt gtgagaaaaa acggagagaa
    15721 aaataaaaag aaaagcgatt attttcatca tttacatcaa tacaagcggt tatttttccg
    15781 aaaatttttg caaattttaa actgagtgtc tatctatcgc tggaaaagct aaggcagagt
    15841 ttcctctgcc ttagaaagat gaattgaatt acattttaag caaacaagcg tttttcgcct
    15901 tcgccggcta cgttttccac acaacgtccg caaacttctt cgtggccaga gtgcgagccg
    15961 atgtcggtag cataatgcca gcaacgaggg catttctcac catctgcatt ttccactttt
    16021 actgcgagtc cggctaattc gccttccgca atatctgctt ccgccagtgg tttaacaatc
    16081 gcttgtgagg taatcagcac aaagcgtaat tcattgccaa gttgttctaa taacgggcga
    16141 atttcatcat tggcgaatac cgtcaccttg gcttctaagc cagcaccaat tactttgtct
    16201 ttacgggctt gttccagcac acggttgact tccgcacgca cttttaagat ttgttgccag
    16261 taagcatcat ctaatttatc ttgctcggtt aaaccgaata atccggtgta gaattcttcg
    16321 gtaaagacaa attcacttct gccttccacc tgcggtaagt agccccaaat ttcatcggct
    16381 gtgaaggata aaatcggtgc aatccaacgt actaacgctt ctgcaatatg ccataacgct
    16441 gtttggcagc tacggcgggc aaggctgtcg gctttggtgg tgtattggcg gtctttgata
    16501 atatctaagt agaaagaccc catttcaatc gagcagaaac gcattaaacg ctgtactacc
    16561 gtgtggaatt ggtagttatc ataggcttct ttgatgtcgt tttgagcctc taaagcacaa
    16621 gccaccgccc agcggtctaa ggcgatcatc tcttgcggtt gaactaaatc actttttgga
    16681 tcgaaaccgt tcaagttcgc caataagaaa cgggctgtgt tacgaatacg gcggtatgat
    16741 tccccggcac tgtttaaaat attgtgcgaa acggtaatct caccggtata gtcggtggag
    16801 gcaacccata agcgtaaaat atctgcaccg tttttgttcc acacttcact tggcacaatt
    16861 acgttgccaa gcgatttgga catttttctg cctttctcat ctacggtaaa accgtgagtt
    16921 agcacttgtt tgtatggtgc tttgccgtta gtagcagtgg aaagcattaa agatgacata
    16981 aaccaaccac gatgttggtc tgaaccttct aaatacatat ccgctgaatt gccgttaaat
    17041 tcagggcgtt gttctacaac agaggcatag gtcgatcctg aatcaaacca cacgtctaag
    17101 gtatccggca ctttgcggta atctttggct tcttcaccga gcaattccgc aggatctaaa
    17161 tcccaccacg cttgaatgcc tttttcttct acacgtttcg ccacttcttc aattaattct
    17221 aaagtgcgag gatgtaattc ttcggtttca ttatgcacaa acatcgccat tggcacgccc
    17281 caagtacgtt ggcgtgaaat acaccagtct gggcggtttg ccaccatcgt atcaatacga
    17341 gcttcacccc agcttgggat ccaacgcacc gatttgattt ctcctagtgc ttgctggcgt
    17401 aagccttggg tttccattcc gataaaccat tgtggggtag cacggaagat aatcggggtt
    17461 ttgtgtctcc aacagtgcgg ataactatgc ttgatgcggg ataattttaa taatgctccc
    17521 acttcttgta atttttcgac tactttatcg ttggattcaa acacacctaa gccggcaaag
    17581 aacggggtgt ctgctttgaa tttaccgtca ttggaaatta gccctgccat ttcaatgtta
    17641 tatttgcgag cgaccacgaa gtcgtccgca ccgtgatccg gtgcagtatg cactaaccct
    17701 gtaccaccgt ctgtggtaac gtggtcgcca aaaataaatg gcacgctgta atcatagaat
    17761 gggtggttaa agcgtaataa ttctaagttt tcgcctttgg tggttgctaa aatctcagca
    17821 gattcaacgc ctgctgcttt tgcaacatct tcgactaaat cgaccgctag taacacacgc
    17881 tcatcaccta attggactaa ggcatagtct aattcagggt tgatcgcaat cgcacggtta
    17941 gacggaatag tccaaggggt agttgtccaa ataaccgcag ataatgcccc tctaccttta
    18001 ccctgtgcgt tgaatttttc ttctaccgca tttacgtcca cagcattaaa acgcacataa
    18061 atagagggag aaactttatc ttcatattcc acttcagctt ccgctaacga agatccacaa
    18121 tctaagcacc aatgtaccgg ttttgaacct ttgtataaat gcccgttcgc aatcactttg
    18181 ccgagcgtgc gaataatatt cgcctcagtg ttaaaattca ttgttaggta tgggttatcc
    18241 caatcaccta aaatacccat acggataaaa tcggctttct gaccttctac ttgttctttc
    18301 gcatagtcac ggcacgcttg gcgaaactcg gcagccgaaa ttttctcatt tggtttacca
    18361 acaatccctt ctactttcag ctcaatcggc aaaccgtgac agtcccagcc cggcacataa
    18421 ggggtatcga aacctaatgc ggttttcgat ttcaaaatca tatcttttaa aattttattc
    18481 actgcgtgac ctaagtgaat attaccgtta gcatacggag gaccatcatg caaaataaag
    18541 gttttcttgc ctttcgaggc agtacgaatt ttttggtata aatttttgtc ataccaattt
    18601 tttaacatat cgggttcacg tttcgcgaga tccccacgca taggaaagcc tgtttcaggc
    18661 aagtttaagg tgtttttgta gtcaatagtc atttttttat ttctcgttaa aattatctgt
    18721 tttatgccta gcacggcata gccgtactag gttaagcata gctcagcccc tctggggctg
    18781 gaaactagaa aggctcacct tgcaaattcc ttaaaaaatt caaccgcttg caagcgatct
    18841 ttctcaatct gggcttttag tgcttcaaag tcgggaaatt ttatttcatt gcgtaatttt
    18901 tttaaaaaga tcacttcaat cgcctcaccg taaatagagg cattgaaatc aaaaatatgc
    18961 acttctaata acggcttagt accgttaatg gtcgggcggt tgccgacatt ggcgattccg
    19021 ttaaaactgc cctgtttggt cttcactttt accgcaaata ccccttgaat cggcgtaacc
    19081 aaacgattga gcataatatt tgcggtagga aaaccaatgg ttctgccgag tttattaccg
    19141 tgtgcaacgc gtcccagaat cgaataaggc ttgccgagta atttctctgc caaatctaac
    19201 tcattgtttt gtaaggcttc tcgcactaat gagctgctaa ttctttcttc gtttaagcta
    19261 tgggtatggc tttcttccac cgcaaagcga tattttaagc cggcattgcg taaagtttca
    19321 aaactgcccg cccgttttgc cccaaagcga aaatcatcgc ctacacttaa atagcgtact
    19381 ttcagtcttt tagcgagcaa atcgctgatg aaatcgtctg ccgaaatttg agcaaatttt
    19441 tcactaaaac gcacgcatag cacaaaatcc acaccggctt cttccaaata ttttagctta
    19501 tctcgcaggc gcattaagcg ggcgggcggg ttacaagcgg ttgaatttcc actttttttt
    19561 gcaaaaaact ctcgaggttg tggctcaaat aacatcacaa ctgagggcaa tcctaaatct
    19621 aaagctctat cacaaagacg agccaaaata ttttgatgcc caagatgcac accatcgaaa
    19681 ttcccgatag agagaacaca gccttgattt aaagcgatgt gtttgtctaa gttgtggaaa
    19741 cctcgaataa gttgcataaa agataaacca ttttataagt gaaaattgct gtgattataa
    19801 cgagaaatct acaaaaaagg aaaaaaattc aatctgttat atttttttag tgctgaaagt
    19861 ggtcgtgtag ctaattatta aagcaaagat atgctataaa actgctattt atacaataaa
    19921 aatatattca aatgctacat tttactatat gataatcatc aaaaaatcga gggaatcatt
    19981 atgacagatt ttgcttatct tcaacaaaag cgcaaacagc ttaaactcaa agtgaatgat
    20041 atttgtgaac aagctggggt aacccgagct tattttaatc agttagtaag cggaaaaatc
    20101 aaaaatccaa gcgccaataa gcttaaagcc ttgcattccg ttttagatat cgtagaagat
    20161 agcaaccagc gttccggggt tatttttggc aaattctatc ctattcacac aggtcatatt
    20221 aatatgatct atgaagcatt tagtaaagta gatgttttac atgttattgt ttgtaccgat
    20281 tctgaacggg atttacgtct tttccaggaa agtaaaatga aacgaatgcc gacaaatgaa
    20341 gatcggctac gctgggtaca gcaaattttt aaataccaac aaaaacagat ctttattcac
    20401 catttagttg aagacggtat tccgagctat ccaaatggct gggaagattg gtctggtcgt
    20461 gttaaagcgc tatttgaaga aaaaagcatt aatcctacta ttgtttttag tagtgaaatt
    20521 caagataaag aatcttatga aaaatatttg aatctagaag ttcaacttgt cgatcctact
    20581 cgtgaatcct ttaatatttc agcaacacaa attcgtaata atccttttca atactggcgc
    20641 tttattccga aagaagttcg tcctttcttt gttaaaacta ttgcgatttt aggtggtgaa
    20701 agcagtggta aatcagtgtt ggtgagcaaa ttagcgaatg tatttaacac tacctctgct
    20761 tgggaatatg ggcgtgagtt tgtgtttgag caacttggtg gcaatgaaca agcaatgcaa
    20821 tattccgact atccgcaaat ggtgcttgga catcaacgct acgttgatta tgccatgaaa
    20881 catgctcata aagtggctat tattgatacc gattacatca caactcaagc gttctgtatt
    20941 cagtatgaag ggaaggctca cccgtttctg gattcaatga tccgcgaata tccttttgat
    21001 gtgacgattt tactttctaa caatacaaaa tgggtagccg atggtttacg cagtttagga
    21061 tcgaaaaaac agcgtcaaca gtttcaacac ctactgaaaa aactactaga aaaacaccat
    21121 attccttata ttaaaattga atctccaagt tatttggatc gctacaatca agccaaagag
    21181 gtggtagaag ctattttaaa tgaagaacca atcccattac agtttaagca atataatatt
    21241 cacgaatttg aataactgcc acaggaaata acatgatact ttttgcaggc gatccacacg
    21301 gtagctacga gcatctttat ccgattctac aacagcagac ggaggaggta gctttagtta
    21361 ttctaggcga tcttcaattg actactcctg atgaattaga tcggctttcg cagtattgtg
    21421 atatttggtt tattcatggt aaccatgaca gtaaaaccgt tgctgccttt gatgctattt
    21481 ggaatagtag ctggaaagaa cgcaatattc acggtaaagt agttgacatt caaggaataa
    21541 aaattgcagg tattggaggg gtcttccgag gtcatatttg gatgccgccc aatcgtccaa
    21601 tgttttttga tcccatccat ttttgccaat actctgctcc ggaaaaaatt tggcgtggtg
    21661 gtattccatt aaggcaccgc tcttcgatct tcccttctga ttttgaagca ttggaaaatg
    21721 aaaaagccga tattttaatt acacacgaag caccaaagcc acaccctcaa ggcttctctg
    21781 taatcaacca gctagcaaga aaattagggg cgagcaaaat tttccatggc cactaccatg
    21841 ataattttaa ttatggcaat ctaaatgtca gcagagaatg tgctatttat aatatcggct
    21901 ttcgtagcct agcggatatt aatggaaatt
//
LOCUS       DANLXF010000008        21576 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.008, whole genome shotgun sequence.
ACCESSION   DANLXF010000008 DANLXF010000000
VERSION     DANLXF010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 21576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21576
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.008"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..167)
                     /locus_tag="PXD66_000185"
     CDS             complement(<1..167)
                     /locus_tag="PXD66_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL6318525.1"
                     /translation="MKAQQLKNAILQLAIQGKLVPQDPNDEPASELLCKIQAEKDRLI
                     AEGKIKKNKKTA"
     gene            complement(373..2034)
                     /locus_tag="PXD66_000186"
     CDS             complement(373..2034)
                     /locus_tag="PXD66_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HDL6318526.1"
                     /translation="MLTWEHIEKMIAHQQFIREKAEIEYKEAKNSVPKDLWHTYSAFA
                     NSNGGFILLGIKENEQGHFPELLISGVNDPDKVIDELFSQSRSEKVSKSLLKDNDVKI
                     FQVENKSVIAIYVKAADILDRPIHLNGDYRLSYVRLNSGDHKLTSSELRSFISSYSQK
                     DADGRIVPYASIDDISLSTLDKYRNLLKAHNSTSKLLTLSDEKLLQEIGAYKKDLAQN
                     SEGITNAGLLMFGKHYAITSIFPHFFFEYYEEAGDVKRYTLRITDFDLEEGNLFEFYL
                     KIVPLIQELGKDKHFKLDNLTRTEENEITDGLREALINAITHADYFNNVRHLKVIKTK
                     DNKLIFENAGVMLVDIPTAIAGQRSECRNSIIHNLFHRLGLCERQGQGVRDIYSYWQS
                     RNFNSPLLESEIDYTKLTLTFQSGMIADFEEKFTACFGENFSELQPIQKDCLLFIAFN
                     QGKAMFAELAQKLPQYPRRNITLALARLKEKGLLTSSGSPRKQLYTLVNLEWIITEKS
                     QDDYFNPPFDQLDKASESKGAPPKKSKVKQVKQLPQNKANKLPPK"
     gene            complement(2047..3516)
                     /locus_tag="PXD66_000187"
     CDS             complement(2047..3516)
                     /locus_tag="PXD66_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HDL6318527.1"
                     /translation="MSLNNIVKRLQDVMRNDAGINGDAQRIEQIVWILFLKIYDAKEQ
                     EWELENDEYHSILPNFLRWQNWAEDKKDGKAMTGDELLSFVNNELFPTLKNLPISADT
                     PMNQRIIRAAFEDNNNYMKNGVLLRQVINIIDEIDFAHYQERHAFGDIYENILKSLQS
                     AGNAGEFYTPRAVTDFMVQAIKPKLGERIADFACGTGGFLTSALKVLESQIQTLSDRT
                     LFNNSVYGIEKKALPHLLCITNLLLHDIDNPNVHHDNALEKSVKDYTENDKFDVILMN
                     PPYGGSEIEQIKKNFPTALQSSETADLFMSVIMYRLKQNGRAAVVLPDGFLFGTDNAK
                     VAIKKKLLEEFNLHTIIRLPHSVFAPYTSITTNILFFDKTEPTRETWVYRLDMPQGYK
                     NFSKTKPMKLEHFNEVVEWWGNRQVIEVEGFDKAKSFTLKEMSDRQYNLDLCGFPHEE
                     EEILPPDELIADYQRKRTAYNADIDKILAEITDILGIKL"
     gene            complement(3564..5915)
                     /locus_tag="PXD66_000188"
     CDS             complement(3564..5915)
                     /locus_tag="PXD66_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL6318528.1"
                     /translation="MYTLQELKQMNEQEIRSRLISPAIRNAGWSDRQIGEEYTFKTNK
                     RFTDGQVVVDPKTTQTKRIEAKRVDYLLYTSANQKIAVVEAKDNHHSSRHGLQQAMTY
                     ARLLDVPFAYSSNGDEFVEHDFITGVQRTLPMSAFPTPDELHQRWAKQYTTEELKIIQ
                     QPYYTSSTDTFKEPRYYQEIAINRTVEAVAKGQQRILLVMATGTGKTYTAFQIIHRLK
                     TSGAKKKILFLADRNVLVDQPMMQDFKPFKDKMVKVSNKKLDSAYEVYLALYQQLDGE
                     TDNELFKQFQPSFFDLIVIDECHRGSAKEDSDWRAILKYFKDATHIGMTATPKETDDA
                     SNITYFGEPIYTYSLKQGIEDGFLAPYRVIRVDLDCDLKGWRPTKGLKDHQGNEIEDR
                     LYTVKDFDYNIVLNDRTRLVAERICEFLRKTDRLQKTIVFCANINHAERMVAELSRLN
                     ADMLKHNSKYVCQITGDQSGKGVDLEEFCDVANEGKDYPVIAVTSKLMTTGVDSKTCK
                     LVVLDANIQSMTEFKQIIGRGTRLRPDFGKEFFTILDFRGVTRLFADPAFDGEPVVIL
                     DVPENKPLVEQPLPKPSDKDIIKDPELKPYKIPVVVDFGTLKLRILDEKVQYLNEQGE
                     LVSEDIDLFSKREMQKEFGSYEQFQQQWTTSGEIFSKFYTDPKWLAALRQTRQFSHDV
                     EDFDVLSHISFGKKPLTKTERAEKVKQSGYVEQYSPENQQVLGLLLNEYVKSGNQDLT
                     KTEILKTPQFSRFGGLVSIIKNFGGKELYQKAVKGLEKMIYSE"
     gene            complement(5987..6880)
                     /gene="psd"
                     /locus_tag="PXD66_000189"
     CDS             complement(5987..6880)
                     /gene="psd"
                     /locus_tag="PXD66_000189"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251643.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HDL6318529.1"
                     /translation="MQLKKYPVPTYFQRVKIALHYLLPQLAITRAAGWLAEQKWGVVT
                     HLIIKLFAKQYKVNLSEAKKTEPSDYATFNEFFIRELKENARPINQDPNTLCLPAEGK
                     VSESGEISDNRLLQAKGHHFTLETLLANDEEMAAKFKDGTFITTYLSPTDYHRVHMPC
                     DGTLRKMIYVPGELFSVNPFLAEHVPNLFARNERVICEFDTAFGPMLQILVGATVTAS
                     MSTVWAGVINPPRADEVVVWNYETEGEKAIKLQKGEEMGAFRLGSTVINLFPKGKVHL
                     NPALIAGMQTRMGEELGRIIN"
     gene            complement(7045..7383)
                     /locus_tag="PXD66_000190"
     CDS             complement(7045..7383)
                     /locus_tag="PXD66_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HDL6318530.1"
                     /translation="MKKIKQLGLTFLFGLNAMAFAQTPPTTMPVEQNAVTQPVQQTQI
                     TNANAVNINTASAAELQDKLVGIGQKKAQAIVDYRTKNGNFKNIEQLAEVSGIGQATV
                     EKNRANIILE"
     gene            7676..8995
                     /locus_tag="PXD66_000191"
     CDS             7676..8995
                     /locus_tag="PXD66_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="opacity-associated protein A"
                     /protein_id="HDL6318531.1"
                     /translation="MTQNNFRKEPVFGESSVTSQDAEATKTDTAQAPYVSLRSTKAPG
                     HTFTPIMKRPDAKVEDHTADVFSAKQAKEFQFTPSSEATEAVEPDNTIHSVEESVSTA
                     SFNAVEAQENVEKEVVKPAPESFTTERIIPNAATAAAAATAAAAATKTKEIKAKMPPK
                     TRRLLLVALLALALLVLFFLLKPSTPETVEELQSQQGGSLPIEFRPVDEAEAKRAEEQ
                     ARAEQEAAEAAKAQEQAQAAQQESVTVAPAQTEQTMAKPSQDSQSQTQSQAVVEQPVA
                     VKPVENKPVVAQPAIKPKTENSVVFQPETTVKQQPKAPAKTEVKTERATLAAKETAKT
                     AAKPVEKAPTKTEAVKAAPAAESANAVATKTMTVPKGVSLMQVFRDNNLNISDVNAMS
                     KVNNVVSNLKVGERVTVRLDKNNRVVEMSIGSGGKFTRQANGSYTFK"
     gene            9113..9475
                     /locus_tag="PXD66_000192"
     CDS             9113..9475
                     /locus_tag="PXD66_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HDL6318532.1"
                     /translation="MLVACSTVVTPVEQIHQVQKEKESVKKVTKLIAQAPKVSLYRCE
                     NDKKVEVERQGRTKKALKKEIVTVSYQGTTHQLSPSVTRDGKKYTNIRWTWHETRSGK
                     AFLYNNTKKTLAANCIKQ"
     gene            9586..10623
                     /gene="waaF"
                     /locus_tag="PXD66_000193"
     CDS             9586..10623
                     /gene="waaF"
                     /locus_tag="PXD66_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HDL6318533.1"
                     /translation="MNILVIGPSWVGDMMMSHALYQQLKIQYPNCQIDVMAPDWCCPL
                     LARMPEVRNAISMPIGHGSFRLCERYRLGKSLRNQYDMAIVLPNSLKSAFIPLFAKIA
                     RRRGWKGESRYFFLNDLRSNKNDYPMMVQRYVALAFEKATVPTAQQLPIAYPYLTTDS
                     EQIEQTKAKFEKQLAYAENRPAIGFCPGAEFGPAKRWPHYHYAILAKMLIEQGYSVRL
                     FGSKKDEAAGEQIRLALPENLQRYCLNLAGQTDLNQAVDLIADCNAVVSNDSGLMHIA
                     AALNKPLVALYGPTSPQYTPPLSQNAEIMRLIEGGLIKIRKGEGAEGYHQSLIDIQPE
                     MVMDKLAELLV"
     gene            10690..11649
                     /gene="rfaC"
                     /locus_tag="PXD66_000194"
     CDS             10690..11649
                     /gene="rfaC"
                     /locus_tag="PXD66_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HDL6318534.1"
                     /translation="MKILIVKTSSMGDVLHTLPAITDMQNAIPDLKVDWVVEENFTEI
                     PAWHSAVNKVIPVAIRRWRKNLCQRQTWVEWQQFLKQLKSEQYDAVIDAQGLIKSAVL
                     VTRQAKGKKFGYDKNSAREGLSSLFYDETFNIPYQQHAVERIRKLCAKSLGYALPTER
                     GDYCIAQHFAKNPQKTTAYIMLIHATTRADKHWQEAYWAELIQALDKHNLAVRLPWGN
                     QAEKERAERLASVANNAKVLPKMSLTDIATQLAGAAAVVSVDTGLSHLAAALDKPNVI
                     LYGATDPKLIGAYGQNQFYLKADKMENITSSQVLEKLLPLINK"
     gene            11770..12144
                     /gene="rpsL"
                     /locus_tag="PXD66_000195"
     CDS             11770..12144
                     /gene="rpsL"
                     /locus_tag="PXD66_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDL6318535.1"
                     /translation="MATINQLVRKPRVKKVVKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRIRLTNGFEVTSYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGAL
                     DCAGVKDRKQGRSKYGVKRPKA"
     gene            12275..12745
                     /gene="rpsG"
                     /locus_tag="PXD66_000196"
     CDS             12275..12745
                     /gene="rpsG"
                     /locus_tag="PXD66_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDL6318536.1"
                     /translation="MPRRRSIEPRKILPDPKFGSELLAKFINVLMVDGKKSTAEKIVY
                     GALETLTQRSGKEALEAFEIALENVRPTVEVKSRRVGGSTYQVPVEVRPTRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAADNKGSAVKKREDVHRMAEANKAFAHFRW"
     gene            12847..14949
                     /gene="fusA"
                     /locus_tag="PXD66_000197"
     CDS             12847..14949
                     /gene="fusA"
                     /locus_tag="PXD66_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDL6318537.1"
                     /translation="MARTTPISLYRNIGISAHIDAGKTTTTERILFYTGVSHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMSQQYPQHRINVIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRTGANFLRVVEQIKT
                     RLGGNSVALQLPIGAEDSFKGVVDLIKMKAINWNEADQGMTFTYEDIPADMLEACEEH
                     RAMLVEAAAEASEELMEKFFSGEELTEDEIKAALRQRVLAGEVIPVCCGSAFKNKGVQ
                     AMLDAVIDYLPAPTDIPAIKGINEDETEGERHASDDEPFASLAFKIATDPFVGNLTFF
                     RVYSGVINSGDTVYNSVKQKRERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVGTG
                     DTLCAIDAPIILERMEFPEPVISVAVEPKTKADQEKMGLALGRLAQEDPSFRVHTDEE
                     SGETIISGMGELHLDIIVDRMKREFKVEANIGKPQVSYRETIRTRVNDVEGKHAKQSG
                     GRGQYGHVVIDLYPLDAEGPGYEFVNEIKGGVIPGEYIPAVDKGIQEQLKSGPLAGYP
                     VVDLGVRLHFGSYHDVDSSELAFKLAASLAFKAAFAKASPVLLEPIMKVEVETPPDYV
                     GDVIGDLSRRRAMVNGQEANEFVVKINAEVPLSEMFGYATDLRSQTQGRASYSMEPLK
                     YAEAPKNVADAIIEARRK"
     gene            15009..16193
                     /gene="tuf"
                     /locus_tag="PXD66_000198"
     CDS             15009..16193
                     /gene="tuf"
                     /locus_tag="PXD66_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDL6318538.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKHFGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTATRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALQALNGVAEWEEKILELANHLDTYIPEPERAIDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIRTGDEVEIVGIKDTAKTTVTGVEMFRKLLDEGRAGENVG
                     ALLRGTKREEIERGQVLAKPGSITPHTDFESEVYVLSKEEGGRHTPFFKGYRPQFYFR
                     TTDVTGTIELPEGVEMVMPGDNIKMTVSLIHPIAMDEGLRFAIREGGRTVGAGVVAKI
                     IK"
     gene            16490..17788
                     /gene="tig"
                     /locus_tag="PXD66_000199"
     CDS             16490..17788
                     /gene="tig"
                     /locus_tag="PXD66_000199"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDL6318539.1"
                     /translation="MSFSIETLEGLKRRVTINVPADKIEAAYKEQLKGYAKNARVDGF
                     RKGKVPHSIIEQRFGIAARQDVLSDEMQRAFFEAVIAEKINLAGRPTFTPNNYQPGQD
                     FSFTAEFEVFPEVELKGLENIEVEKPVVEIADADLDKMVDVLRKQQATWSESQEAAKA
                     ESRVTIDFVGSVDGEEFEGGKASDFVLFMGQGRMIPGFEDGIVGRKAGEQFDIDVTFP
                     EEYHAENLKGKAAKFAITLKKVEEMVLPELTEEFVKKFGNAKSVEELRAEIKKNMQRE
                     LKNAVTSRVKTQVINGLIAQNDVEVPAAAVEEEINVLRQQAVQRFGGKPEMAAQLPAE
                     LFEAEAKRRVQVGLLLSSIIGSNELKVDEKRVEEMIADIASAYEQPAEVVAHYAKNRQ
                     LTENIRNVVLEEQAVDAVLAKAKVTEKTMSFDEVMSQQAQ"
     gene            17904..18356
                     /locus_tag="PXD66_000200"
     CDS             17904..18356
                     /locus_tag="PXD66_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL6318540.1"
                     /translation="MEQLTFAQQLEQFFANHTLMVFAWVALLIAVIFSFYKSATQKYK
                     IVDNAQATQLINKEEAVVVDLRSDEEFRAGHIIDSIHVLPSDIKAAKINQIDKYKARP
                     VIVVDTNDFTSGGVAADLAKQGFSRVYVLKEGIGGWRAASLPTIKKHK"
     gene            18377..18877
                     /gene="secB"
                     /locus_tag="PXD66_000201"
     CDS             18377..18877
                     /gene="secB"
                     /locus_tag="PXD66_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HDL6318541.1"
                     /translation="MSEENQVASTEEATQTPFELQIQRIYVKDVSFEAPNLPTIFQQE
                     WKPQLGFELDTETKEIGEDLYEVTLHITVQTTLEDSNDVAFICEVKQAGVFTIKGLEG
                     IQLAHCLASQCPNVLYPYARELISSLVNRGTFPALNLSPVNFDALFMDYLQRQEESAE
                     EPALAN"
     gene            18984..19994
                     /gene="gpsA"
                     /locus_tag="PXD66_000202"
     CDS             18984..19994
                     /gene="gpsA"
                     /locus_tag="PXD66_000202"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDL6318542.1"
                     /translation="MSEIYSAPITVLGAGSYGTALAIALSRNGHKTYLWGHNPDKMAV
                     LASERMNQDFLPDIVFPEALEIVADLATAVQKSKDLLIVVPSHVFSEVLAQIKPFLSQ
                     DHRIMWATKGLERDTGRLLQTVAKEILGEIYPLAVLSGPTFAKELAAGLPTAISLAST
                     DPVFADQMQKRSHCSKAFRVYLNNDIIGVQLGGAIKNVIAIGAGISDGMGFGANARTA
                     LITRGLAEISRLGVSLGADPKTFTGMAGLGDLVLTCTDNQSRNRRFGLALGQGKSAEV
                     AIAEIGQVVEGFYNTKEAYLLAQTQGVEMPIVEQIYQMLFCGKQASDVVKTLLGRERK
                     GE"
     gene            20010..20825
                     /gene="cysE"
                     /locus_tag="PXD66_000203"
     CDS             20010..20825
                     /gene="cysE"
                     /locus_tag="PXD66_000203"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDL6318543.1"
                     /translation="MQQSEINQIWQAIRDEAKELANCEPMLASFFHATILKHHNLGDA
                     LSYILANKLENPIMPAIALKEIIEEAYRAEPQIIASAACDINAVRTRDPAVDKWSTPL
                     LYLKGFHALQSYRVTHYLWNQGRKALAVYLQNEISVAFDVDIHPAAKVGCGIMFDHAT
                     GIVVGETSVIENDVSILQGVTLGGTGKENGDRHPKIREGVMIGAGAKILGNIEIGRYS
                     KIGANSVVLQPVPDHATAAGVPARIIGKSSEQKPAFDMNQYFEDEQGLFGDGI"
     gene            20874..21485
                     /locus_tag="PXD66_000204"
     CDS             20874..21485
                     /locus_tag="PXD66_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDL6318544.1"
                     /translation="MQLTFNDFQNYYNGKGDLTMFSFYGKKTWENGIYVGIDGSIGRA
                     NNKLSHQSQTIKLARNVYSLGLTVGKTLEVASFNLQPSFGMRYHHLTSANAQLENTKF
                     ETDRVDLLAIQAGLALNKTFIFNELKVKPELGSYFVDASHGTLKTKLNEFSLNQNIGR
                     YFKQEVGITLHYKGVSSSVHAGFTKGNTLQEQKFISLKVGYEW"
BASE COUNT         6536 a         4126 c         4629 g         6285 t
ORIGIN      
        1 gcagtttttt tgtttttttt gatctttcct tctgcaatca agcggtcttt ttctgcttgg
       61 attttgcaaa ggagttcgct tgccggttca tcatttggat cttgtggaac gagcttgcct
      121 tggattgcta gttgtaggat tgcgtttttt agctgttggg ctttcatttt ttattctcaa
      181 taatcggttg ttatttctta aagtatcttt gattacggtg ttttgggttt tctgggaatt
      241 gcatttcgat cacaccaagc gttagtgcag gttttaaata ctgcatacgg aaatgcttat
      301 catcttttaa cctgagtttt ttcataattt ctttgctctt catcggctca agcaacactg
      361 aaagtaagcg atttacttcg ggggtaattt gtttgctttg ttctggggta attgtttgac
      421 ttgcttcact ttgcttttct tcgggggggc gcccttactc tcactagctt tatcaagttg
      481 gtcgaaaggt ggattaaaat aatcatcttg tgatttctct gtgattatcc attctaaatt
      541 cactagggta tagagttgtt ttctcggact ccccgaactg gttagtaagc ctttttcttt
      601 cagtcgtgct agtgccaaag taatatttct gcgtggatat tggggcagtt tttgtgctaa
      661 ttctgcaaac attgctttac cttgattgaa ggcgataaag agtaagcaat ctttttgaat
      721 aggctgtaac tcagagaaat tttcgccaaa gcaagcggta aatttttcct caaaatctgc
      781 aatcatacct gattgaaaag ttaaggtgag tttagtgtaa tcaatttcac tttctaaaag
      841 cggtgaattg aaatttcggc tttgccaata tgaataaata tcccgaacgc cttgtccttg
      901 tcgctcacat agccctaaac ggtggaataa gttatgaata atagaattac ggcattccga
      961 tctttgccct gcaatagccg tgggaatatc aaccaacatt actcctgcat tttcaaaaat
     1021 cagtttgtta tctttggttt taatgacttt tagatggcga acattgttaa agtaatctgc
     1081 gtgagtaatt gcattgatta gagcttcccg tagcccgtca gtaatttcat tttcctcagt
     1141 acgggttaaa ttatctaatt tgaaatgttt atctttacca agctcttgaa taagtggcac
     1201 aattttcaag taaaattcaa agagattgcc ttcttcaaga tcaaaatcgg taatccgtaa
     1261 tgtatagcgt tttacatcac cagcttcttc gtaatattca aaaaagaagt ggggaaatat
     1321 gcttgtaatg gcatagtgct taccaaacat caataagcct gcattggtaa tgccctcgct
     1381 attctgtgct aaatcttttt tataagcccc aatttcttgc agcaatttct catcggataa
     1441 cgtaagtaat tttgatgtgc tgttatgggc tttcagcaga tttctgtatt tatccaatgt
     1501 gcttaaactg atatcatcaa tagaagcata aggcacaatt ctcccatcag catctttttg
     1561 gctatagctt gaaatgaaag aacgcagttc agaggaggtg agtttatgat cgcctgaatt
     1621 taaacgaaca taagaaagtc gataatcacc atttaaatgg ataggacggt ctaaaatatc
     1681 agctgctttc acataaattg caatcacgct tttattttca acttgaaaga ttttcacatc
     1741 attatccttt aataaggatt tactgacttt ttcactacga gattgggaaa ataattcatc
     1801 aatgacttta tcaggatcat taacaccaga aattagcagt tcaggaaaat gtccttgttc
     1861 attttccttt attcctaata aaataaagcc accattgctg tttgcaaaag ccgaataagt
     1921 atgccaaaga tcttttggca cactattttt tgcctcttta tattcaattt ctgccttttc
     1981 tcggataaat tgctgatgtg caatcatttt ttcgatatgt tcccaagtca gcattttatg
     2041 ctcctattac agtttaatgc ctaaaatatc ggtaatttct gctagaatct tatcaatatc
     2101 agcattgtaa gcggtgcgtt ttcgctgata atctgcaatt agttcatcag gcggaaggat
     2161 ttcctcttct tcgtgaggaa agccacataa atcgaggttg tattgacggt cggacatttc
     2221 ctttaatgta aaacttttcg ctttgtcaaa cccttcgact tcaatcactt ggcgatttcc
     2281 ccaccattca acaacttcgt tgaaatgttc caatttcatc ggtttagttt tggagaaatt
     2341 tttataaccc tgtggcatat caaggcgata aacccaagtt tcacgggttg gctcggtttt
     2401 atcaaaaaac agaatattag tcgtaatcga ggtataaggg gcaaagacac tatgcggtaa
     2461 gcggataatc gtatgcagat taaactcttc taatagcttt ttcttaatcg caactttggc
     2521 attatctgtg ccgaacaaaa atccgtcagg cagcaccacc gcagcccgtc cattctgttt
     2581 taggcggtac ataattaccg acataaacag atcagcggtt tcactgctct gtaaagccgt
     2641 agggaaattc tttttaattt gctcaatttc tgaacctccg taaggtggat tcatcaaaat
     2701 cacatcaaat ttatcatttt cggtgtaatc tttgaccgat ttttctagag cattatcgtg
     2761 atgtacattc ggattatcaa tatcgtgcaa caataaattg gtaatacaaa gcaggtgagg
     2821 aagggctttt ttctcaatgc catacacaga attattgaat aaagtgcggt cagaaagggt
     2881 ttgaatttga ctttccaata cctttaacgc cgaagttaaa aaaccacccg tcccacaggc
     2941 aaaatcggca atgcgttcgc caagtttcgg tttgattgct tgtaccataa aatcggttac
     3001 agcgcgaggg gtataaaatt cccctgcatt acctgcactt tgtaaacttt tgaggatatt
     3061 ttcataaata tcgccaaagg cgtgtcgttc ttggtaatgg gcaaaatcaa tttcatcaat
     3121 gatattgata acctgacgta ataacacccc atttttcata tagttgttat tatcttcaaa
     3181 agcagcacgg ataatccgtt ggttcatcgg cgtatcggct gaaataggca gattttttaa
     3241 agtcgggaaa agttcgttat tgacgaaaga gagtaattca tcaccagtca tcgctttgcc
     3301 gtctttttta tcttccgccc aattttgcca gcgtaaaaaa ttcggcaaaa ttgagtgata
     3361 ttcatcgttt tccagttccc actcttgttc tttagcatcg taaattttca agaataagat
     3421 ccagacgatc tgttcaatgc gttgggcatc accgttaatc cctgcatcgt tacgcattac
     3481 atcttgcagg cgttttacta tgttgttgag gctcattgtg atccttgttt atctgtaaaa
     3541 ttttgttgta aaataaccgc ttgttattcc gaataaatca tcttttctaa gccttttacc
     3601 gctttctggt agagttcttt accgccgaaa tttttaataa tagacactag acctccaaaa
     3661 cggctgaatt gcggtgtctt taaaatttcg gttttagtca gatcttgatt gccggatttg
     3721 acatattcat taagcagcaa ccctaaaact tgctggtttt cgggcgaata ctgctccaca
     3781 tagccacttt gtttcacttt ttctgctcgt tcggttttag tcagtggttt tttgccaaag
     3841 gagatgtggc ttaatacatc aaagtcttca acatcgtggc tgaattggcg tgtttggcga
     3901 agggctgcca gccattttgg atcggtgtaa aatttcgaga aaatttcacc gcttgttgtc
     3961 cattgctgtt ggaactgttc gtaactgccg aactcttttt gcatttcccg ttttgagaac
     4021 agatcaatat cttcagaaac cagctcgccc tgctcattca gatattggac tttctcgtct
     4081 aaaatgcgta atttgagtgt gccgaaatcg acaactaccg ggattttata tggctttagc
     4141 tcggggtctt taatgatgtc cttatcactg ggtttaggaa gcggttgttc gacaagtggc
     4201 ttattttccg gtacatctaa aatcaccacc ggctcaccat caaatgccgg atcagcaaac
     4261 aggcgagtaa cgcctcgaaa atctaaaatg gtgaaaaatt ctttgccgaa atcagggcga
     4321 agacgagtgc ctcgtccgat aatttgttta aattccgtca ttgattgaat gtttgcatca
     4381 agtacaacca gcttacaggt tttgctatct acacctgttg tcatcaattt agaggttacg
     4441 gcaatcacag ggtaatcttt cccttcattt gctacatcac aaaattcttc taaatcaacg
     4501 cctttgcctg attggtcgcc tgtaatttgg cagacatatt tggaattgtg tttcaacata
     4561 tcggcattga ggcgtgaaag ctctgccacc attcgctctg catggttgat gttggcacag
     4621 aagactattg ttttctgcaa gcggtcggtt ttgcgtaaaa attcgcaaat tctctccgca
     4681 accaagcggg tgcgatcatt tagcacaatg ttgtaatcga aatctttgac tgtatagagg
     4741 cgatcttcaa tctcattacc ttgatgatct tttaaacctt tggttggtcg ccagcctttt
     4801 aaatcacagt ccagatcaac acgaatcact cgataagggg caagaaagcc gtcttcaata
     4861 ccttgtttga ggctataggt ataaatcggc tcgccaaaat aggtaatgtt gctggcatca
     4921 tcggtttctt tgggggttgc agtcataccg atatgggtgg cgtccttgaa atacttcaaa
     4981 atagcacgcc agtcagaatc ttcttttgct gaaccacgat gacattcatc gatcacaata
     5041 agatcaaaga aagagggttg gaactgctta aacagttcgt tatcggtttc accgtcaagc
     5101 tgttgataaa gtgcaagata gacttcataa gcactgtcga gctttttatt gctgactttc
     5161 accattttgt ctttaaacgg tttgaagtct tgcatcattg gctgatcgac tagtacattg
     5221 cgatcggcaa gaaataaaat tttctttttc gcaccactgg tttttaaacg atggataatt
     5281 tggaaagccg tataggtttt acccgtgcct gttgccatta ccaataaaat cctctgctga
     5341 ccttttgcta cagcttcaac agtacggtta atcgcaattt cttggtagta acgaggctct
     5401 ttaaaggtgt cagtgctact ggtgtaataa ggctgttgaa taattttcag ctcttcggtg
     5461 gtatattgtt tcgcccaacg ttggtggagt tcgtcgggag taggaaatgc tgacatcgga
     5521 agggttcgtt gtacgccagt gataaaatcg tgttccacaa actcatcacc atttgagcta
     5581 tatgcaaaag ggacatcaag caatcgagcg tatgtcattg cttgctgtaa gccgtgtcgg
     5641 gaagagtgat ggttatcttt ggcctcgacc acggcaattt tttgatttgc ggaagtatag
     5701 agcagataat caacccgttt tgcttcgatg cgtttagttt gcgtcgtttt tggatcaaca
     5761 actacttgcc catctgtaaa tcgtttattg gttttaaatg tgtactcttc gccaatttgt
     5821 ctatccgacc atcctgcatt tctaattgca ggagagatta atcgagagcg aatttcttgc
     5881 tcgttcatct gctttaattc ttgaagtgtg tacatatttt tccttccgat aagagtttct
     5941 tatcggaaga ttatagcaaa cagtttaggt aaaattaact caggatttaa ttgataattc
     6001 tacccaactc ttcccccatt cgggtttgca ttcctgcaat aagtgcaggg ttgagatgta
     6061 ctttaccttt cgggaagagg ttaattacgg ttgagcctaa acggaaagct cccatttctt
     6121 cgcctttttg gagtttgatg gctttttcgc cttcggtttc gtaattccaa actaccactt
     6181 catcagcgcg aggcggatta attacgccag cccaaaccgt actcatactt gcggtaaccg
     6241 ttgcaccgac caaaatctgt agcattggtc cgaatgcggt gtcaaattca caaatcacac
     6301 gctcattgcg agcgaataag tttggaacgt gttcggctaa aaacggattg acggagaaca
     6361 attcgcccgg cacataaatc attttacgca atgttccatc gcacggcata tgtacacgat
     6421 ggtaatcagt tggcgataag taggtggtaa taaatgtgcc gtctttaaat tttgccgcca
     6481 tttcttcatc atttgccaat aaggtttcta gcgtaaaatg atgccctttt gcttgtaata
     6541 aacggttatc cgaaatttcg cctgattcgc tgactttacc ttctgccggt aagcaaagtg
     6601 tatttggatc ttgattaatc gggcgtgcgt tttcttttaa ttcacggata aaaaattcat
     6661 taaaagtagc ataatctgac ggctcggttt ttttagcttc agataaattg actttatact
     6721 gttttgcaaa cagtttgatg ataaggtgag taactacgcc ccatttttgc tctgctaacc
     6781 aacctgccgc acgggtaatt gccagttgtg gcagtaaata gtgcagagcg attttcacac
     6841 gttggaaata ggttggaact ggatattttt ttaattgcat tgcgttttcc ttttatttaa
     6901 aaacgagaat ttgcgtttaa ttgggggcga agtatagcaa atacaatgca aaatagataa
     6961 aaaatcttta aggtgttgaa gtggctacaa agaaaaaaac agataggggc tttgtaggaa
     7021 ttccacttca acaatttggt tgtcttattc taaaataata ttggctcgat ttttttctac
     7081 cgttgcttgc ccaattccgg agacttcagc aagttgctca atgtttttaa aattgccgtt
     7141 tttagtacgg taatcgacaa tcgcttgagc ttttttctgc ccaattccca ctagcttgtc
     7201 ttgcaattca gcagcactag cagtattgat atttaccgca ttagcgttgg tgatttgtgt
     7261 ttgctgtacg ggttgagtga ctgcattttg ttcaacaggc attgtagttg gcggtgtttg
     7321 ggcaaaagcc attgcattta agccaaacaa gaatgttaga ccaagttgtt tgattttctt
     7381 cataggattt ccttattagt tgttgaaagg tcgaaaggaa gattaagagg gaataaaata
     7441 gaactcaacc ggcaaaaata aatttttcga cacagatcga aaaaatagaa aatggctaaa
     7501 cttctagacg tgcatttggt ctgaaattca atgaaagtaa atagatttgc aaaaaattag
     7561 gcaaaaatga ccgctagttg cgtaaattta gttaaataaa ttcaaatagc ggctgaattg
     7621 ctttagaata tcgccatttt tatactcatt aacaacgcta ttatttggaa ttaatatgac
     7681 acaaaataat tttcgcaaag aaccggtttt tggtgaatct tcggttacct ctcaagacgc
     7741 tgaagcaacc aaaacagata cagctcaagc cccttatgtt tcgttgcgtt caaccaaagc
     7801 accggggcat acgtttaccc caattatgaa aagacctgat gctaaagtag aagatcatac
     7861 ggctgatgtc ttttcggcta agcaggcaaa agaattccaa tttactccaa gttcagaagc
     7921 tacagaagca gtagagccgg ataataccat tcattcggtt gaagaaagtg tatcaaccgc
     7981 atcttttaac gcggttgaag cacaagaaaa tgtggaaaaa gaagtggtta aacctgcacc
     8041 cgagagtttc actactgagc gtattattcc aaatgcggca acagctgccg cagcagccac
     8101 tgcggcagct gcagccacta aaaccaaaga gattaaagct aaaatgccac ctaaaacccg
     8161 ccgtttatta ttagtggcat tgttggcgtt agcattattg gtactgtttt tcttgttaaa
     8221 accaagtacg ccggaaactg tcgaagagct acaatctcag caaggtggta gcttgccgat
     8281 tgaattccga ccggttgatg aagctgaagc gaaacgtgcg gaagaacaag ctcgtgcaga
     8341 acaagaagcc gctgaagctg caaaagctca agaacaagct caagccgctc aacaagagtc
     8401 tgtaacagtt gctccagccc aaaccgagca aacaatggct aaaccgtcac aagatagcca
     8461 gtcgcaaaca caatcacaag cagtagttga gcaacctgtt gcggtgaaac cggtggaaaa
     8521 taaaccggtt gttgcacaac cggcgattaa gcctaaaacg gaaaatagcg tggtgttcca
     8581 acctgaaaca acggtaaaac aacagcctaa agcccctgct aaaactgaag tgaaaaccga
     8641 acgtgctaca ctggcagcaa aagaaacggc aaaaacagca gctaaaccag ttgagaaagc
     8701 tccgacaaaa actgaagcgg taaaagcagc accggctgcc gagtcggcaa atgcggtagc
     8761 aaccaaaaca atgactgtgc caaaaggtgt atcactaatg caagtgttcc gtgataacaa
     8821 tttaaatatc tcagacgtta atgcgatgag caaagtcaat aacgtggtga gtaatttaaa
     8881 agtaggcgag cgagtaaccg ttcgtttaga taaaaataac cgtgtggttg aaatgagcat
     8941 tggctcaggc ggcaaattta cccgtcaagc aaacggtagc tatactttta aataaataaa
     9001 atgaagaaaa agagcaacgc tacaaacgtt gctcttttat ttaaaaagga gataaaggat
     9061 gaatttattt tgcccctctt acaaacttat ctacttaacg ctaagctcag cgatgttagt
     9121 tgcttgtagt acagtggtta cccctgttga gcaaattcat caagtacaaa aggaaaaaga
     9181 gtcagtaaag aaagtgacta aactgatagc acaagcacct aaagtgagtt tatatcgttg
     9241 tgagaatgat aagaaggttg aagttgaacg ccagggacga acaaaaaaag cgttaaaaaa
     9301 ggaaatagtc acagtgagtt atcaagggac aacgcatcag ctttcacctt ctgtaacgag
     9361 agatggtaaa aaatacacta atatccgttg gacttggcat gaaacccgta gtggtaaagc
     9421 atttttgtat aacaacacta aaaaaacgtt agcggcaaat tgcataaagc agtaacaagc
     9481 ggacacaaaa agtatcgctt tttgaacgtt ggctttgcca acggcagcga agctgtgaac
     9541 aatccttaca aggattgtga ataaattttt aagagaattt tgcaaatgaa tattttagtt
     9601 ataggaccgt cttgggtggg tgatatgatg atgtcgcacg cactttatca acagcttaaa
     9661 atacagtatc cgaattgcca aattgatgta atggcacccg attggtgttg tccgctttta
     9721 gcccgtatgc ctgaggtgcg taatgcgatt tcaatgccga ttggacacgg ttcatttcgt
     9781 ttgtgtgaac gctaccgact cggaaaatcc ctgagaaatc aatatgatat ggcgattgta
     9841 ttgcctaatt cattaaagtc tgcctttatt ccactgtttg cgaaaattgc gagacgtcgt
     9901 ggttggaaag gggaaagtag atattttttc ttaaatgatc ttcgcagtaa taaaaacgac
     9961 tacccaatga tggtgcaacg ctatgtagcc ttggcgtttg aaaaggctac tgttccaacc
    10021 gcacaacagt tgccgattgc ttatccatac ctcacaactg atagcgaaca aattgagcag
    10081 accaaagcga aatttgaaaa acagttggct tatgcggaaa accgtcctgc catcggcttt
    10141 tgtccagggg ctgaatttgg accggcaaaa cgttggccgc attatcatta tgcaatattg
    10201 gcaaaaatgt tgattgagca aggctattcg gtacggttat ttggctcgaa aaaagatgaa
    10261 gccgccggtg agcaaattcg cttggcatta cctgaaaatt tacaacgcta ttgcctgaat
    10321 ttagccgggc aaaccgattt aaaccaagcg gtagatttga ttgcagattg taatgcagtc
    10381 gtgagcaatg actccggctt aatgcacatt gccgcagcac taaataaacc gctggtcgcc
    10441 ttatatggac cgaccagccc acaatatacg ccgccgttat cccaaaatgc cgagatcatg
    10501 cgcttaattg aaggcggttt gattaaaatc cgcaaaggcg aaggggcaga gggctatcac
    10561 caaagtttaa ttgatattca gcctgaaatg gtgatggaca aattagctga actgttagtt
    10621 taaatggata acaagcggtt gaatttgcca attttttgca aaaaaagacc gcttgtttgt
    10681 ttataaaaga tgaaaatttt aatcgttaaa acctcatcaa tgggagatgt attacatacg
    10741 cttccagcca taaccgatat gcaaaatgct attcccgatt taaaagtaga ttgggtggtg
    10801 gaggagaatt ttaccgaaat tcctgcgtgg cattcagcgg taaataaagt aattccggtg
    10861 gctattcgcc gctggcggaa aaacctttgc caaagacaga cttgggtaga atggcagcag
    10921 tttttaaaac agctaaaaag tgagcagtat gatgcggtaa ttgatgctca agggctgatt
    10981 aaaagtgcgg tgttagtaac tcgccaagcc aaaggcaaaa aatttggcta tgataaaaac
    11041 tctgcccgag aagggttaag tagtctgttt tacgatgaaa cgtttaatat cccctaccaa
    11101 caacacgctg tagaacgcat cagaaaatta tgtgcaaaat cattaggtta tgctttgcca
    11161 accgaacggg gcgattattg cattgcacaa cattttgcaa aaaatcctca aaaaacgacc
    11221 gcttacatta tgctgattca cgccactacc cgagcggata aacattggca agaagcctat
    11281 tgggcggagt tgattcaagc gttggataag cacaatctgg cagtcagatt gccttgggga
    11341 aaccaagccg aaaaagaacg ggcggagcgt ttagcgagtg tggcgaataa tgctaaggta
    11401 ttgccaaaaa tgagcttaac cgatattgct actcaacttg cgggagcagc ggcagtggtg
    11461 tcggtggata ccggcttgag ccatttggcg gctgcgttag ataagccaaa tgtgatttta
    11521 tatggggcaa ccgatccaaa acttatcggg gcttacgggc aaaatcagtt ttatctaaaa
    11581 gcagataaaa tggaaaatat tacctcttca caagtgctag aaaaattact tccattgatt
    11641 aacaaatgat cattgttgtt ctcttactgt gcttgacttt tatccacgtt caatttagaa
    11701 ttcaccgtcc tttcgtgtga aaggatgttt ttaattttat ttttaataat tttaactgga
    11761 gcttttttaa tggcaactat caaccagcta gtacgcaaac cgcgtgtgaa aaaggttgta
    11821 aaaagtaacg ttcctgcatt agaggcttgc ccgcagaaac gtggcgtgtg tactcgtgta
    11881 tacactacta cacctaaaaa accgaactca gcattacgta aagtatgtcg tattcgttta
    11941 acaaatggct ttgaagtaac ttcatacatc ggtggtgaag gtcacaacct tcaagaacac
    12001 agtgttgtat taatccgtgg tggtcgtgtt aaagacttac cgggtgttcg ttaccatact
    12061 gtacgtggtg cacttgactg tgcaggcgtt aaagatcgta aacaaggtcg ttctaaatac
    12121 ggcgttaaac gtcctaaagc ttaatggttc tccgttgagt aaggccaaac gtctaaattt
    12181 taatatttaa accataaact ccagtcaaga tgccttaagg cagattagat gagttttgga
    12241 tatcctgaag atttaatata cggagaaatt tccaatgcca cgtcgtcgta gtattgaacc
    12301 acgcaaaatt cttccagatc cgaagttcgg ttcagaatta cttgcaaaat ttattaacgt
    12361 gttaatggta gatggtaaaa aatctactgc agaaaaaatc gtttacggtg cattagaaac
    12421 tttaacacaa cgttctggta aagaagcgtt agaagctttc gaaatcgcat tagaaaacgt
    12481 acgtccaact gttgaggtta aatctcgccg tgtgggtggt tctacttacc aagttcctgt
    12541 agaagttcgc ccaacccgtc gtaatgcttt agcaatgcgt tggatcgtag aagccgctcg
    12601 taaacgtggt gacaaatcaa tggcattacg tttagctaac gaattatcag atgcagcaga
    12661 caacaaaggt tcagcagtga agaaacgtga agacgttcac cgtatggctg aagctaacaa
    12721 agcgtttgct cacttccgtt ggtaatcgtt taagcaaact tatcaaaggc ttcatctcag
    12781 tttgcggtga agccttaccc ttattaagaa ttttaattta aaccccaaac aaggtaataa
    12841 ataaaaatgg ctcgtacaac ccctatttca ctttaccgta acatcggtat cagtgcgcac
    12901 atcgatgcgg gtaaaacaac tactacagag cgtatcttgt tctatacagg tgtaagtcac
    12961 aaaatcggtg aagttcacga tggtgctgca acaatggact ggatggaaca agaacaagag
    13021 cgtggtatca cgatcacatc tgctgcaaca acagcattct ggtctggtat gtcacaacaa
    13081 tatccacaac accgtatcaa cgttattgat actccgggac acgttgactt tactatcgaa
    13141 gtagaacgtt caatgcgtgt acttgatggt gcggtgatgg tttattgtgc agttggtggt
    13201 gttcaaccac aatctgaaac tgtatggcgt caagctaaca aatataaagt gccacgtatc
    13261 gcgttcgtaa acaaaatgga ccgtacaggt gcaaacttct tacgtgttgt tgagcaaatc
    13321 aaaacgcgtt taggtggtaa ctcagttgca ttacaacttc caatcggtgc agaagacagc
    13381 ttcaaaggtg ttgttgattt aatcaaaatg aaagcaatca actggaacga agctgatcaa
    13441 ggtatgacct tcacttatga agatattccg gcagatatgc ttgaagcttg tgaagaacac
    13501 cgtgcaatgc ttgttgaagc agcagctgaa gcttctgaag aattaatgga aaaattcttc
    13561 tctggtgaag aattaactga agatgaaatc aaagcagcat tacgtcaacg tgtattagca
    13621 ggtgaagtaa tccctgtttg ctgtggttct gcatttaaaa acaaaggtgt acaagcaatg
    13681 cttgatgcgg taatcgatta cttaccggca ccaactgata ttcctgcaat taaaggtatc
    13741 aatgaagatg aaactgaagg tgagcgtcac gcaagcgacg atgagccatt tgcttcatta
    13801 gcattcaaaa ttgcaacaga cccattcgta ggtaacttaa ccttcttccg tgtttactct
    13861 ggtgtaatta actctggtga tacagtttac aactcagtaa aacaaaaacg tgaacgtttt
    13921 ggtcgtatcg tacagatgca cgcaaacaaa cgtgaagaga tcaaagaagt tcgtgcgggc
    13981 gatatcgctg cagcaatcgg tttaaaagat gttggtacag gtgacacatt atgtgcaatc
    14041 gatgcaccaa tcattcttga gcgtatggaa ttccctgagc cggtaatctc tgttgcggtt
    14101 gaaccaaaaa ccaaagctga ccaagagaaa atgggcttag ctttaggtcg tttagcacaa
    14161 gaagaccctt cattccgtgt tcacacagat gaagaatctg gtgaaacgat catttcaggt
    14221 atgggtgagt tacacttaga catcatcgtt gaccgtatga aacgtgagtt taaagtggaa
    14281 gcaaatatcg gtaaaccaca agtatcttac cgtgaaacta tccgtactcg tgttaatgat
    14341 gttgaaggta aacacgcaaa acaatctggt ggtcgtggtc aatatggtca cgttgtgatc
    14401 gacttatacc cattagatgc tgaaggtccg ggttatgagt ttgtaaacga gatcaaaggt
    14461 ggtgtaatcc ctggtgaata cattcctgcg gttgataaag gtatccaaga acagcttaaa
    14521 tctggtccgt tagcaggtta cccggtagta gatttaggtg tgcgtttaca cttcggttca
    14581 taccacgatg ttgactcatc tgaacttgcg tttaaacttg ctgcatcatt agcatttaaa
    14641 gcggcgttcg caaaagcaag cccggtatta cttgagccaa tcatgaaagt ggaagttgaa
    14701 actccaccgg attacgtggg tgatgtaatc ggtgacttaa gccgtcgtcg tgcaatggtt
    14761 aacggtcaag aagcgaacga gttcgttgtt aagatcaatg ctgaagttcc actttcagaa
    14821 atgtttggtt atgcaacaga cttacgttca caaactcaag gtcgtgcatc atactctatg
    14881 gaacctttaa aatatgctga agcaccgaaa aacgttgctg atgcaattat tgaagctcgt
    14941 agaaaataat ttgtttaacc tgagccatag caatagggct atggctcatt ttttaggaag
    15001 atattaaagt gtctaaagaa aaatttgaac gtacaaaacc gcacgttaat gtgggtacaa
    15061 tcggccacgt tgaccatggt aaaacaactt taacagcagc aatcactacc gtattagcaa
    15121 aacacttcgg tggtgcagct cgtgcatttg accaaatcga taacgcgcca gaagaaaaag
    15181 cgcgtggtat caccatcaac acttcacacg ttgagtacga tacagcaact cgtcactacg
    15241 cacacgttga ctgcccggga cacgcggact atgttaaaaa catgattacc ggtgcggcac
    15301 aaatggacgg cgctatctta gtagtagcag caactgacgg tccaatgcca caaactcgtg
    15361 agcacatctt attaggtcgc caagtaggtg taccatacat catcgtattc ttaaacaaat
    15421 gcgacatggt agatgacgaa gagttattag aattagttga gatggaagtg cgtgaacttc
    15481 tttcacaata cgacttccca ggtgatgaca ctccaatcgt acgtggttca gcgttacaag
    15541 cgttaaacgg cgtagcagag tgggaagaga aaatccttga attagcaaac cacttagaca
    15601 catacattcc agagcctgag cgtgcgattg ataaaccatt cttattacca atcgaagacg
    15661 tattctcaat ttcaggtcgt ggtacagtag taacaggtcg tgttgagcgt ggtatcatcc
    15721 gcactggtga tgaagttgaa atcgtaggta tcaaagatac agcgaaaaca accgtaacag
    15781 gtgttgagat gttccgtaaa ttattagacg aaggtcgtgc gggtgagaac gtaggtgcat
    15841 tattacgtgg tactaaacgt gaagaaatcg aacgtggtca agtattagcg aaaccgggtt
    15901 caatcactcc acacacagac ttcgaatcag aagtttacgt attatcaaaa gaagaaggtg
    15961 gtcgtcacac tccattcttc aaaggttacc gtccacagtt ctacttccgt acaacggacg
    16021 taacaggtac aatcgaatta ccggaaggtg tagagatggt aatgccgggc gacaacatca
    16081 aaatgactgt tagcctaatt cacccaatcg cgatggacga aggtttacgt tttgcgattc
    16141 gtgaaggtgg tcgtacagta ggtgcgggcg ttgtagctaa aatcatcaaa taattaagat
    16201 tgtttgataa attgaaaagc taaataaaaa ctagaaagag gcatatcgaa aggtatgcct
    16261 cttttgcttt tatgccgatt aagctagcca tatcatatca tttcatttgc aaaaatcggt
    16321 gaaaaaagac cgcttgtagg caagcccatt gacaagcagt cagattttag ggatttttta
    16381 caaaactgtt tatttgcgaa tgagtttctt ctcgacagtt tcttgataga acgctacaat
    16441 aaccgtctat ttgtatatct tttttatatc cctaaaccga ggtttaagaa tgtcattttc
    16501 aattgaaaca ttagaaggct taaaacgccg tgttactatt aacgtaccag ctgataaaat
    16561 cgaagcagca tacaaagaac aattaaaagg ctatgcgaaa aatgctcgtg tagatggctt
    16621 ccgtaaaggt aaagtgccac actcaattat cgaacaacgt tttggtattg cagcccgtca
    16681 agatgtgtta tctgatgaaa tgcaacgtgc attctttgaa gccgtcattg ctgaaaaaat
    16741 caacttggca ggtcgcccta ctttcacgcc aaacaactac caaccaggtc aagacttcag
    16801 ctttactgcg gaatttgaag tattcccaga agtggaatta aaaggtttag agaatatcga
    16861 agtagaaaaa ccggttgttg aaatcgcaga cgctgattta gacaaaatgg tagatgtatt
    16921 acgcaaacaa caagcgactt ggtcagaaag ccaagaggct gcaaaagccg aatctcgtgt
    16981 aactattgat ttcgttggtt cagtagatgg tgaagaattc gaaggcggta aagcatcaga
    17041 cttcgtgtta tttatggggc aaggtcgtat gatcccgggg tttgaagacg gaatcgtagg
    17101 tcgtaaagcg ggtgagcaat tcgacattga tgtgactttc ccagaagaat accacgctga
    17161 aaacttaaaa ggcaaagcag cgaaatttgc aattaccttg aaaaaagtag aagagatggt
    17221 attaccggaa ttaaccgaag agtttgtgaa aaaattcggt aatgcaaaat cagtggaaga
    17281 attacgcgca gaaattaaga aaaatatgca acgtgaactt aaaaatgcag tcacttcacg
    17341 tgtgaaaact caagtaatca acggcttaat tgctcaaaat gatgttgaag tgccggctgc
    17401 tgcggttgaa gaagaaatca acgtattacg ccaacaagcg gtgcaacgtt ttggtggtaa
    17461 gcctgaaatg gcagctcaat taccggctga attatttgaa gcagaagcta aacgccgtgt
    17521 tcaagtgggc ttattattat cttcaatcat cggttctaac gaattaaaag tagatgaaaa
    17581 acgtgttgaa gagatgatcg cagatatcgc ttcagcttac gaacaaccgg ctgaagtggt
    17641 tgctcactat gcgaaaaacc gtcaattaac tgaaaatatc cgcaatgtgg tattagaaga
    17701 gcaagcggtt gatgcagtgc tagcgaaagc gaaagtgact gaaaaaacaa tgtcatttga
    17761 cgaagtaatg tcacaacaag ctcaataatt tccttataag gtgggctttt gctcaccttt
    17821 tattactttt acaagcggtt aaatttagtg ataaatttgc atcaaaacga ccgcttattt
    17881 tttgatgtta aggaaattga attatggaac aactaacttt tgctcaacaa ttagagcaat
    17941 tttttgccaa tcataccttg atggtgtttg cttgggttgc attattgatt gcggtaattt
    18001 ttagctttta taaaagtgca acgcaaaaat ataaaattgt cgataacgca caggctacac
    18061 agctgattaa taaagaagag gcagtagtgg ttgatttacg ttctgatgaa gagttccgtg
    18121 ccggtcatat tattgatagc attcacgtgt taccgtcaga cattaaagca gctaaaatca
    18181 atcagattga taaatacaaa gctcgcccgg taattgtagt agacactaat gacttcactt
    18241 caggtggtgt tgcggcagat cttgctaaac aaggctttag cagagtttat gtattaaaag
    18301 aagggattgg cggctggcgt gcagcaagcc taccaactat taaaaaacat aaataatcta
    18361 ttattaagga aattttatgt cagaagaaaa tcaagttgca tcaacagaag aagctacaca
    18421 aacccctttt gagcttcaaa ttcaacgcat ttatgtaaaa gatgtttcat ttgaagcacc
    18481 aaatttaccg actattttcc aacaagagtg gaaaccacaa ttaggctttg agttagatac
    18541 tgaaaccaaa gaaattggtg aagatttata cgaagttacc ttacacatta cggtgcaaac
    18601 tacgttagaa gacagcaatg atgtcgcttt catttgtgaa gtaaaacaag caggtgtgtt
    18661 tactattaaa ggtttagaag gcattcaact tgctcactgc ttagcttcac aatgtccgaa
    18721 tgtgctttat ccttatgctc gtgagctaat ctcaagcctt gtgaatcgtg gtacattccc
    18781 ggcattaaat ctctcaccgg taaactttga tgcgttattt atggattatc tccaacgcca
    18841 agaagaaagt gcagaagagc cggctttagc gaactaagca ttttagatag aaaataggta
    18901 tgcaaggcat acctattttt ttacaagcgg ttattttttc tactattttt tgcaattttc
    18961 taaataagcc gaacagagga aatatgagtg aaatctattc tgccccaatc acggtattag
    19021 gtgcaggttc ttatggaaca gctttagcca ttgccctttc tcgaaatggt cacaaaacct
    19081 atttatgggg gcataatccc gacaaaatgg ccgttttggc atcagagcga atgaaccaag
    19141 attttctgcc tgatattgta ttcccggaag ccttagaaat tgtcgcagat cttgctacag
    19201 cggtacaaaa atcgaaagat ctgctgattg tggtgccaag tcatgtattc tctgaagtat
    19261 tggctcaaat caagcctttt ttgtcccaag atcaccgtat aatgtgggca accaaagggc
    19321 tggagcgaga taccgggcgt ttgttgcaaa cggtagctaa ggaaattttg ggggaaattt
    19381 acccgcttgc ggtgctttcc ggtccgactt ttgccaaaga actggcggca ggcttgccaa
    19441 cagcgatttc gcttgcctca actgatcctg tttttgccga tcaaatgcaa aaacggagtc
    19501 actgttccaa agcgttccga gtttacctca acaatgatat tatcggtgtg caactcggtg
    19561 gggctattaa aaatgtgatt gcaattggtg ccggtatttc agacggaatg ggattcgggg
    19621 cgaatgctcg taccgccttg attactcgag ggctggcgga gattagccgt ttgggtgttt
    19681 cgctcggggc tgatccgaaa acctttaccg gaatggcggg gcttggagat ttagtgctga
    19741 cgtgtaccga taaccaatcc cgaaaccgcc gatttggttt ggcgttaggg caaggaaaat
    19801 cggcagaagt cgcgatagcg gaaatcggtc aagtggtaga aggattttat aataccaaag
    19861 aagcctattt gctcgctcaa acacagggcg ttgaaatgcc gattgtagaa caaatttacc
    19921 aaatgttatt ttgtggcaaa caggcttcgg atgtggtgaa aaccttgttg gggcgtgaac
    19981 gaaaaggcga ataaagaaag gagaataaaa tgcagcagag tgaaattaat cagatttggc
    20041 aagcaattcg agatgaagcc aaagagctgg cgaattgtga acctatgtta gcaagttttt
    20101 tccacgctac cattttaaaa caccataatc taggcgatgc gttaagttat attttggcga
    20161 ataagttaga aaacccgatt atgccggcaa tcgccttaaa agaaattatt gaggaagctt
    20221 atcgagcaga gccacaaatt attgctagtg cagcttgtga tattaatgcg gtcagaacta
    20281 gagatcctgc ggtggacaaa tggagtacac ctctgctgta cttaaaaggc ttccacgctt
    20341 tacaaagcta tcgggtgacg cattatcttt ggaatcaagg caggaaagca ttagcggttt
    20401 atttacaaaa tgaaatttct gttgcctttg atgtggatat tcatcctgcg gcaaaggtcg
    20461 gttgcggaat tatgtttgac cacgccaccg gtattgtggt gggtgaaacc tctgtgatcg
    20521 aaaatgatgt ctcgatttta caaggtgtaa cactaggcgg tacagggaaa gaaaatggcg
    20581 accgccaccc gaaaatccgt gaaggcgtga tgattggagc cggtgcaaaa attctcggta
    20641 atattgaaat cggacgctat tctaaaatcg gggcaaattc agtggtgcta caacctgtgc
    20701 ctgatcacgc cacagcagca ggtgtaccgg ctcgcattat cggcaaatcc tctgaacaaa
    20761 aaccggcttt tgatatgaat caatactttg aagatgagca aggtttgttt ggtgatggga
    20821 tttaggtaag agttattcct ttaatttatg atttagacat tgctaagtac aagttgcaat
    20881 taacctttaa tgattttcag aactactata atggtaaagg ggatttaact atgttttctt
    20941 tttatggtaa gaaaacatgg gaaaatggca tttatgtagg gattgatggt agcattgggc
    21001 gtgcgaataa caaattaagc catcaaagcc agacgataaa actagccaga aatgtatatt
    21061 ctttaggatt aacggtaggt aaaacgttgg aagttgcaag ttttaaccta caaccgtcat
    21121 ttggtatgcg ctatcatcat ttaacatctg caaatgccca attagagaac acaaaatttg
    21181 agactgatag ggtagatctt ttggccattc aagctggttt agcattaaat aaaactttta
    21241 tatttaatga attaaaggta aaacctgaat taggaagcta ttttgttgat gcaagccacg
    21301 gaacacttaa aactaagtta aatgaatttt ccttaaatca aaatataggc cgttatttta
    21361 agcaagaagt agggattaca ctccactata aaggggtatc aagttcggta catgctggct
    21421 ttacaaaagg aaatacatta caagaacaaa aattcatttc actcaaagtt ggatatgagt
    21481 ggtaagcttt atgtaacctg aagctacact tgaatcggtt caaacataat gttactaaaa
    21541 aagccttgaa acattgattt tcaaggcttt ttaggt
//
LOCUS       DANLXF010000009        20505 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.009, whole genome shotgun sequence.
ACCESSION   DANLXF010000009 DANLXF010000000
VERSION     DANLXF010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 20505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20505
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.009"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..492
                     /locus_tag="PXD66_000205"
     CDS             <1..492
                     /locus_tag="PXD66_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HDL6318545.1"
                     /translation="YFNLLGNITWLWMNSSLHKEWSCELLARNVIPAIENEQYMLLID
                     NGIPIAYCSWADLNLETEVKYIKDINSLTPEEWQSGDRRWIIDWVAPFGHSQLLYKKM
                     CQKYPDMIVRSIRFYPKQKELGKIAYFKGGKLDKKTAKKRFDTYQEELATALKNEFNF
                     IKK"
     gene            508..3369
                     /locus_tag="PXD66_000206"
     CDS             508..3369
                     /locus_tag="PXD66_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX family hemolysin"
                     /protein_id="HDL6318546.1"
                     /translation="MGTRLTTLSNGLKNTLTATKSGLHKAGQSLTQAGSSLKTGAKKI
                     ILYIPQNYQYDTEQGNGLQDLVKAAEELGIEVQREERNNIATAQTSLGTIQTAIGLTE
                     RGIVLSAPQIDKLLQKTKAGQALGSAESIVQNANKAKTVLSGIQSILGSVLAGMDLDE
                     ALQNNSNQHALAKAGLELTNSLIENIANSVKTLDEFGEQISQFGSKLQNIKGLGTLGD
                     KLKNIGGLDKAGLGLDVISGLLSGATAALVLADKNASTAKKVGAGFELANQVVGNITK
                     AVSSYILAQRVAAGLSSTGPVAALIASTVSLAISPLAFAGIADKFNHAKSLESYAERF
                     KKLGYDGDNLLAEYQRGTGTIDASVTAINTALAAIAGGVSAAAAGSVIASPIALLVSG
                     ITGVISTILQYSKQAMFEHVANKIHNKIVEWEKNNHGKNYFENGYDARYLANLQDNMK
                     FLLNLNKELQAERVIAITQQQWDNNIGDLAGISRLGEKVLSGKAYVDAFEEGKHIKAD
                     KLVQLDSANGIIDVSNSGKAKTQHILFRTPLLTPGTEHRERVQTGKYEYITKLNINRV
                     DSWKITDGAASSTFDLTNVVQRIGIELDNAGNVTKTKETKIIAKLGEGDDNVFVGSGT
                     TEIDGGEGYDRVHYSRGNYGALTIDATKETEQGSYTVNRFVETGKALHEVTSTHTALV
                     GNREEKIEYRHSNNQHHAGYYTKDTLKAVEEIIGTSHNDIFKGSKFNDAFNGGDGVDT
                     IDGNDGNDRLFGGKGDDILDGGNGDDFIDGGKGNDLLHGGKGDDIFVHRKGDGNDIIT
                     DSDGNDKLSFSDSNLKDLTFEKVKHNLVITNSKKEKVTIQNWFREADFAKEVPNYKAT
                     KDEKIEEIIGQNGERITSKQVDDLIAKGNGKITQDELSKVVDNYELLKHSKNVTNSLD
                     KLISSVSAFTSSNDSRNVLVAPTSMLDQSLSSLQFARAA"
     gene            3444..5570
                     /locus_tag="PXD66_000207"
     CDS             3444..5570
                     /locus_tag="PXD66_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_063101322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HDL6318547.1"
                     /translation="MEANHQRNDLGLVALTMLAQYHNISLNPEEIKHKFDLDGKGLSL
                     TAWLLAAKSLALKAKHIKKEISRLHLVNLPALVWQDNGKHFLLVKVDTDNNRYLTYNL
                     EQDAPQILSQDEFEACYQGQLILVTSRASVVGQLAKFDFTWFIPAVIKYRKIFLETLI
                     VSIFLQIFALITPLFFQVVMDKVLVHRGFSTLNIITVALAIVIIFEIVLSGLRTYVFS
                     HSTSRIDVELGAKLFRHLLSLPISYFENRRVGDTVARVRELDQIRNFLTGQALTSVLD
                     LLFSFIFFAVMWYYSPKLTLVILGSLPCYILWSIFISPILRRRLDEKFARSADNQAFL
                     VESVTAINMIKAMAVAPQMTDTWDKQLASYVSSSFRVTVLATIGQQGVQLIQKTVMVI
                     NLWLGAHLVISGDLSIGQLIAFNMLSGQVIAPVIRLAQLWQDFQQVGISVTRLGDVLN
                     SPTEQYQGKLSLPEIKGDISFKNIRFRYKPDAPTILNNVNLEIRQGEVIGIVGRSGSG
                     KSTLTKLLQRFYIPENGQVLIDGHDLALADPNWLRRQIGVVLQDNVLLNRSIRENIAL
                     SDPGMPMERVIYAAKLAGAHDFISELREGYNTIVGEQGAGLSGGQRQRIAIARALVNN
                     PKILIFDEATSALDYESEHIIMQNMQKICQGRTVILIAHRLSTVKNADRIIVMEKGEI
                     VEQGKHHELLQNSNGLYSYLHQLQLN"
     gene            5582..7018
                     /locus_tag="PXD66_000208"
     CDS             5582..7018
                     /locus_tag="PXD66_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_140441352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HDL6318548.1"
                     /translation="MKIWLSGIYEFFLRYKNIWAEVWKIRKELDHPNRKKDESEFLPA
                     HLELIETPVSKKPRLIAYLIMLFLVVAIVLASVSKVEIVATAPGKLTFSGRSKEIKPI
                     ENAIVQEIFVKDGQFVEKGQLLVSLTALGSDADIKKTMASLSLAKLENYRYQTLLTAI
                     EKESLPVIDLSRTEFKDSSEEDRLRIKHLIEEQYTTWQKQKTQKTLAYKRKEAEKQTI
                     FAYVRKYEGATRIEQEKLKDFKALYKQKSLSKHELLAQENKLIEAQNELAVYRSKLNE
                     LENDLLNVKEELELITQFFKSDVLEKLKQHIENERQLRLELEKNNQRRQASMIRAPVS
                     GTVQQLKIHTIGGVVTTAETLMIIVPEDDVLEATALVPNKDIGFVAAGQEVIIKVETF
                     PYTRYGYLTGRIKHISPDAIEQPNVGLVFNATIAIDRKNLTSPDGRKIDLSSGMTITA
                     EIKTGERSVMSYLLSPLEESVTESLRER"
     gene            7259..8257
                     /locus_tag="PXD66_000209"
                     /pseudo
     CDS             7259..8257
                     /locus_tag="PXD66_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250818.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            8274..9017
                     /locus_tag="PXD66_000210"
     CDS             8274..9017
                     /locus_tag="PXD66_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL6318549.1"
                     /translation="MKQKYIYGLAGLLLLLLGWQLGTALLSQRLPMASMLAPAKTWES
                     FTWLISSGEIFPHLWASLQRIAVGLLAALVIGVPLGLLLGFSKHLEMLCTPSFQLLRM
                     ISPLSWMPIIVMLLGVGDLPIYFLLAFATVWSLIISTTSGVKSIDPQWLQLGRVMSAT
                     RKELLCKIVVPAITGHVLTGLRVAVGIAWVVLVPCEMLGVNQGLGYFILDTRDRLAYS
                     ELMAVIIIIGLIGWALDSLIRLALAKGGR"
     gene            9172..10230
                     /locus_tag="PXD66_000211"
     CDS             9172..10230
                     /locus_tag="PXD66_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HDL6318550.1"
                     /translation="MSVLSSSFIQWLQQNAENLDQSNERADLLIPQIAALGLYKIGVP
                     ETLGGSGGTLVDAIKAVTELGNHSLAAAFVSWGHRTFIEHIIASKNPIPRQSYLADLL
                     NGNLTAGTGLSNAMKFLSGIEELNVNIREENGKRYLNGRLPWVTNLRSYRFVASFVAG
                     FQDERKPLVIAIPSTAKGLTRTKELEFIALQGTNTTSLVFENVELQDEWILADDAPEF
                     LAATRPAFLGLQFGLPFGLVERSLAEIEKSLPFRAILASEWQAQVDALNDIKTRLFNG
                     LNEEGYFVRNPKELFQARIDIVNVVAQTILLELQASGGRGYLKNDTSGFSRRWKEAAF
                     LPVVTPSAVQLRMVLANS"
     gene            complement(10351..10893)
                     /locus_tag="PXD66_000212"
     CDS             complement(10351..10893)
                     /locus_tag="PXD66_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDL6318551.1"
                     /translation="MSQFQIHTIESAPETAQEALKAVQQANGFIPNLIGVLANAPTAL
                     ETYRTVGGINSRNSLTAEEREVVQITAAVVNGCGFCVAGHTKIALKALKMPEEVVNAL
                     RATARIDDNKLDTLARFTLAVMLQKAKLTEAQLNEFFAAGYNQQNAIDVILGVSLATL
                     CNYVNNIAEIPINPELQPFA"
     gene            complement(11045..12556)
                     /gene="glpK"
                     /locus_tag="PXD66_000213"
     CDS             complement(11045..12556)
                     /gene="glpK"
                     /locus_tag="PXD66_000213"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDL6318552.1"
                     /translation="MEKQYIIALDQGTTSSRAVLLDKNANVVEVAQREFTQIYPQAGW
                     VEHNPMEIWSSQSSTLNEVVAKAGIKPDSIAAIGITNQRETTIVWEKETGKPVYNAIV
                     WQCRRTSDITDKLKADGHEEYIRKTTGLVVDPYFSGTKVKWILDNVEGAREKAERGEL
                     LFGTVDTWLVWKLTQGRVHVTDYTNASRTMLFNIHTKQWDDKMLELLNIPRSMLPEVK
                     NSSEVYGQTNIGGQGGVRIPVAGIAGDQQAALYGHLCVKEGQAKNTYGTGCFMLMHTG
                     NTAIQSENGLLTTIACNAKGEPEYALEGSVFIAGASIQWLRDELKIVHDSFDSEYFAT
                     KEDDSNGVYVVPAFTGLGAPYWDPYARGSIFGLTRGANRNHIVRATLESIAYQTRDVL
                     EAMQSDSKAKLQALRVDGGATANNFLMQFQADILDTKVERPKVKEVTALGAAYLAGIA
                     VGFWKDLEELKDKAEIERTFTPDGDQEKRAKRYKGWKKAVRRSLEWAKEDAEE"
     gene            complement(12560..13345)
                     /locus_tag="PXD66_000214"
     CDS             complement(12560..13345)
                     /locus_tag="PXD66_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDL6318553.1"
                     /translation="MSKSLRNACIGEFIGTGFILFFGAGCVAAAQVAGANFGLWEISI
                     VWGLGVSMAIYISAGISGAHLNPAVTIALAAFYGFEKHKILPYVIAQVAGAFCSVALI
                     YFMYSDLFTAAEAAQGITRGETVGFAGVFSTYPNPNITLVTAFIVEFVITVVLMSTIL
                     AIGDDKNGLPNKALAALLIGLLIAVIGGATGPLTGFAMNPARDFGPKLFAYLAGWGEI
                     ALTGGKEIPYFIIPIVAPICGALFGAWGYKNLIHNNLPANTQE"
     gene            13558..13917
                     /locus_tag="PXD66_000215"
     CDS             13558..13917
                     /locus_tag="PXD66_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HDL6318554.1"
                     /translation="MISIGAVLGAMCRWQLAVWLNPILSQFAFGTLAANWLGCLLIGI
                     AMGFNLGESQRLLFISGFLGSFTTFSSFSLELTEKLLAEKWGQFVTVLSLHLIGGLLL
                     TLIGVMLARAVTSGRFC"
     gene            complement(13965..15287)
                     /locus_tag="PXD66_000216"
     CDS             complement(13965..15287)
                     /locus_tag="PXD66_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HDL6318555.1"
                     /translation="MSAMFIIQFAIVLLCILVGARVGGIGLGVFGGLGLAILSFGFGL
                     KPAGLPIDVMFMIMAVVSAAAAMQAAGGLDYMIKIATRILRKNPKYITFIAPLVTWTF
                     TVLAGTGHVAYSVLPVIAEVSRHNGIRPERPLSMAVIASQFAIVASPIAAAVVAVVAF
                     LEPQGVTLGNVLSVTIPATLLGLGLACVFVNKMGKELKDDPNYQRLLQDPEYVKENHI
                     TTNPTELPLKPTAKLSVGLFLFGALLVVLMGAIPALRPVFDGKPMGMAHTIEIVMLSI
                     GALIIFFCKPDGNEITKGSVFHAGMRAVIAIFGIAWLGDTLMQGHMDEVKSMVSGLVE
                     TAPWAFAFALFVLSVLVNSQGATVATLFPVAIAIGIPAPVLIGVFVAVNGYFFIPNYG
                     PIIASIDFDTTGTTRIGKYIFNHSFMLPGLLSMIFSIGFGLLLSNILL"
     gene            15436..16260
                     /gene="dapF"
                     /locus_tag="PXD66_000217"
     CDS             15436..16260
                     /gene="dapF"
                     /locus_tag="PXD66_000217"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDL6318556.1"
                     /translation="MQFSKMHGLGNDFMVVDGVTQNVYLTEEMIRTLSDRHRGIGFDQ
                     LLLVEPPYDPELDFHYRIFNADGSEVSQCGNGARCFARFVTLKGLTNKKDIHVSTAKG
                     KMVLSLQEDGKVRVNMGEPIWEPSQVPFTANKFEKNYILRTELQTVLCGVVSMGNPHC
                     VLQVDDVKTAPVNELGPLLESHERFPERANISFMQVVNRNHVKLRVYERGAGETQACG
                     SGACGTVAVGIMQGLLDNKVQVDLPGGTLIIEWQGVGHPLYMTGDATHIYDGFIKL"
     gene            complement(16431..16973)
                     /locus_tag="PXD66_000218"
     CDS             complement(16431..16973)
                     /locus_tag="PXD66_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase family protein"
                     /protein_id="HDL6318557.1"
                     /translation="MAQVYIVHGYTANADKHWFPWLEQELAKCGVSCERLNMPDSAKP
                     SLDGWLNHLAQNVELTDQTVFVGHSLGCIAILNFLAKNYAKIKGAVFVSGFYQPVENL
                     PELSAFTNYYAILPSVSSFPSYVVSALDDKVVNHQYSDALAQHLHADYIRLNQGGHFL
                     DREGVTELPVVLELVKKLLE"
     gene            17097..17420
                     /locus_tag="PXD66_000219"
     CDS             17097..17420
                     /locus_tag="PXD66_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QacE family quaternary ammonium compound efflux
                     SMR transporter"
                     /protein_id="HDL6318558.1"
                     /translation="MNVWILLAISICLEIAATNLLKLSNGFTKAIPTIGSLALYGLSF
                     YFLSIIFRTLPVGIVYAIWSGVGIVLTALVAYFAFGQKIDLAGLIGISLIIAGVLVIN
                     LFSKV"
     gene            complement(17483..18928)
                     /gene="xylB"
                     /locus_tag="PXD66_000220"
     CDS             complement(17483..18928)
                     /gene="xylB"
                     /locus_tag="PXD66_000220"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HDL6318559.1"
                     /translation="MYIGIDLGTSGVKVVLLDENQHIIAITQKSLPIFRPHPLWSEQN
                     PQDWWNATNEAMLELSATQDLQAVKAIGLTGQMHGATLLDNADNVLSPAILWNDGRSF
                     MECEELEQLVPNSREITGNLMMPGFTAPKLRWVDKHQPDIAEKVSKVLLPKDYLRLLM
                     SGEYASDMSDASGTMWLDVGKRNWNKSLLNACGLDIENMPKLFEGNQITGYLRPTLAE
                     QWKMKSVPIVAGGGDNAAGAIGIGLYQTGQAMLSLGTSGVYFVVSDKFQANPQKAVHS
                     FCHALPDRWHLMSVILSAASAVDWAVKSLGLKDVSTLFQQVEASQTASDAIFLPYLSG
                     ERTPHNDPYAKGVFWGLSHNDNQITMAKAVIEGVSFALAEGIEVLHETGVVADNIALI
                     GGGAKSVYWRQLLADISGKTFEYRTSGDVGPALGAAKLAQIALNPNQDIATFCQPLPL
                     EQVYQPTPARYAEYQQKRQKFTELYRRLKGL"
     gene            complement(19081..20400)
                     /gene="xylA"
                     /locus_tag="PXD66_000221"
     CDS             complement(19081..20400)
                     /gene="xylA"
                     /locus_tag="PXD66_000221"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012072255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HDL6318560.1"
                     /translation="MSNYFDKISKVQYEGANSTNPFAFKHYNPDEVVLGKTMAEHLRL
                     AVCYWHTFCWTGNDMFGVGSLDRSWQKTGDLLQGAKQKAEIAFEFFQKLGIPYYCFHD
                     VDVAPEGNNFKEYQHNFNTIVDILEQKQAETGVKLLWGTANCFTNPRYMSGASTNPNP
                     EVFAWAAAQVFTAMNATKRLGGENYVLWGGREGYETLLNTDLKREREQIGRFMQLVVE
                     HKHKIGFNGTLLIEPKPQEPTKHQYDYDVATVYGFLKQFGLEKEIKVNIEANHATLAG
                     HTFQHEIATATALDIFGSIDANRGDPQLGWDTDQFPNSVEENTLVMYEILKAGGFTTG
                     GFNFDAKIRRQSTDPYDLFHAHIGAIDVLALSLKRAAKMLEDQALQKVVDNRYAGWNQ
                     DLGLQILNGKASLEDLAKIVETQGLDPKPVSGQQEYLENLVNSYIYR"
BASE COUNT         6070 a         4187 c         4378 g         5870 t
ORIGIN      
        1 tattttaact tactaggaaa cattacttgg ctatggatga actcctccct ccacaaagaa
       61 tggagctgtg aactactagc acgcaatgtg attcctgcaa ttgaaaatga acaatatatg
      121 ctacttatag ataacggtat tccgatcgct tattgtagtt gggcagattt aaaccttgag
      181 actgaggtga aatatattaa ggatattaat tcgttaacac cagaagaatg gcagtctggt
      241 gacagacgct ggattattga ttgggtagca ccattcggac attctcaatt actttataaa
      301 aaaatgtgtc agaaataccc tgatatgatc gtcagatcta tacgctttta tccaaagcag
      361 aaagaattag gcaaaattgc ctactttaaa ggaggtaaat tagataaaaa aacagcaaaa
      421 aaacgttttg atacatatca agaagagctg gcaacagcac ttaaaaatga atttaatttt
      481 attaaaaaat agaaggagac atcccttatg ggaactagac ttacaaccct atcaaatggg
      541 ctaaaaaaca ctttaacggc aaccaaaagt ggcttacata aagccggtca atcattaacc
      601 caagccggca gttctttaaa aactggggca aaaaaaatta tcctctatat tccccaaaat
      661 taccaatatg atactgaaca aggtaatggt ttacaggatt tagtcaaagc ggccgaagag
      721 ttggggattg aggtacaaag agaagaacgc aataatattg caacagctca aaccagttta
      781 ggcacgattc aaaccgctat tggcttaact gagcgtggca ttgtgttatc cgctccacaa
      841 attgataaat tgctacagaa aactaaagca ggccaagcat taggttctgc cgaaagcatt
      901 gtacaaaatg caaataaagc caaaactgta ttatctggca ttcaatctat tttaggctca
      961 gtattggctg gaatggattt agatgaggcc ttacagaata acagcaacca acatgctctt
     1021 gctaaagctg gcttggagct aacaaattca ttaattgaaa atattgctaa ttcagtaaaa
     1081 acacttgacg aatttggtga gcaaattagt caatttggtt caaaactaca aaatatcaaa
     1141 ggcttaggga ctttaggaga caaactcaaa aatatcggtg gacttgataa agctggcctt
     1201 ggtttagatg ttatctcagg gctattatcg ggcgcaacag ctgcacttgt acttgcagat
     1261 aaaaatgctt caacagctaa aaaagtgggt gcgggttttg aattggcaaa ccaagttgtt
     1321 ggtaatatta ccaaagccgt ttcttcttac attttagccc aacgtgttgc agcaggttta
     1381 tcttcaactg ggcctgtggc tgctttaatt gcttctactg tttctcttgc gattagccca
     1441 ttagcatttg ccggtattgc cgataaattt aatcatgcaa aaagtttaga gagttatgcc
     1501 gaacgcttta aaaaattagg ctatgacgga gataatttat tagcagaata tcagcgggga
     1561 acagggacta ttgatgcatc ggttactgca attaataccg cattggccgc tattgctggt
     1621 ggtgtgtctg ctgctgcagc cggctcggtt attgcttcac cgattgcctt attagtatct
     1681 gggattaccg gtgtaatttc tacgattctg caatattcta aacaagcaat gtttgagcac
     1741 gttgcaaata aaattcataa caaaattgta gaatgggaaa aaaataatca cggtaagaac
     1801 tactttgaaa atggttacga tgcccgttat cttgcgaatt tacaagataa tatgaaattc
     1861 ttactgaact taaacaaaga gttacaggca gaacgtgtca tcgctattac tcagcagcaa
     1921 tgggataaca acattggtga tttagctggt attagccgtt taggtgaaaa agtccttagt
     1981 ggtaaagcct atgtggatgc gtttgaagaa ggcaaacaca ttaaagccga taaattagta
     2041 cagttggatt cggcaaacgg tattattgat gtgagtaatt cgggtaaagc gaaaactcag
     2101 catatcttat tcagaacgcc attattgacg ccgggaacag agcatcgtga acgcgtacaa
     2161 acaggtaaat atgaatatat taccaagctc aatattaacc gtgtagatag ctggaaaatt
     2221 acagatggtg cagcaagttc tacctttgat ttaactaacg ttgttcagcg tattggtatt
     2281 gaattagaca atgctggaaa tgtaactaaa accaaagaaa caaaaattat tgccaaactt
     2341 ggtgaaggtg atgacaacgt atttgttggt tctggtacga cggaaattga tggcggtgaa
     2401 ggttacgacc gagttcacta tagccgtgga aactatggtg ctttaactat tgatgcaacc
     2461 aaagagaccg agcaaggtag ttataccgta aatcgtttcg tagaaaccgg taaagcacta
     2521 cacgaagtga cttcaaccca taccgcatta gtgggcaacc gtgaagaaaa aatagaatat
     2581 cgtcatagca ataaccagca ccatgccggt tattacacca aagatacctt gaaagctgtt
     2641 gaagaaatta tcggtacatc acataacgat atctttaaag gtagtaagtt caatgatgcc
     2701 tttaacggtg gtgatggtgt cgatactatt gacggtaacg acggcaatga ccgcttattt
     2761 ggtggtaaag gcgatgatat tctcgatggt ggaaatggtg atgattttat cgatggcggt
     2821 aaaggcaacg acctattaca cggtggcaag ggcgatgata ttttcgttca ccgtaaaggc
     2881 gatggtaatg atattattac cgattctgac ggcaatgata aattatcatt ctctgattcg
     2941 aacttaaaag atttaacatt tgaaaaagtt aaacataatc ttgtcatcac gaatagcaaa
     3001 aaagagaaag tgaccattca aaactggttc cgagaggctg attttgctaa agaagtgcct
     3061 aattataaag caactaaaga tgagaaaatc gaagaaatca tcggtcaaaa tggcgagcgg
     3121 atcacctcaa agcaagttga tgatcttatc gcaaaaggta acggcaaaat tacccaagat
     3181 gagctatcaa aagttgttga taactatgaa ttgctcaaac atagcaaaaa tgtgacaaac
     3241 agcttagata agttaatctc atctgtaagt gcatttacct cgtctaatga ttcgagaaat
     3301 gtattagtgg ctccaacttc aatgttggat caaagtttat cttctcttca atttgctaga
     3361 gcagcttaat ttttaatgat tggcaactct atattgtttc acacattata gagttgccgt
     3421 tttattttat aaaaggagac aatatggaag ctaaccatca aaggaatgat cttggtttag
     3481 ttgccctcac tatgttggca caataccata atatttcgct taatccggaa gaaataaaac
     3541 ataaatttga tcttgacgga aaagggcttt ctttaactgc ttggctttta gctgcaaaat
     3601 cgttagcgtt gaaagcgaaa cacattaaaa aagagatttc ccgcttacac ttggtgaatt
     3661 taccggcatt agtttggcaa gataacggta aacatttttt attggtaaaa gtggataccg
     3721 ataataaccg ctatttaact tacaatttgg aacaagatgc tccacaaatt ctgtcacaag
     3781 acgaatttga agcctgctat caagggcagt taattttggt cacgtccaga gcttccgtag
     3841 taggtcaatt agcaaagttc gatttcacct ggtttattcc ggcggtgatc aaataccgaa
     3901 aaatctttct agaaaccttg attgtttcga tctttttgca aatttttgcc ctaattacac
     3961 cgctattctt ccaagttgtt atggataaag tactggtgca tcgaggtttt tcaaccttga
     4021 atatcattac ggttgcctta gctattgtga tcatctttga aattgtacta agtggtttga
     4081 gaacctatgt tttttctcat agcactagcc gtattgatgt tgaattaggc gctaaattat
     4141 ttcgacattt attatcacta cccatttctt attttgaaaa cagacgagtt ggagatacag
     4201 tcgctagggt tagagaatta gatcaaattc gtaatttcct taccggacaa gcattaacct
     4261 cggtgttaga tctcttattc tcttttatct tttttgccgt aatgtggtat tacagcccaa
     4321 aattaacctt ggtaattctt ggttcattgc cctgctatat tttatggtca atttttatta
     4381 gtccgatttt aagacggcgt ttagatgaga aatttgcccg aagtgctgat aaccaagcat
     4441 tcttagttga gtcggtaaca gccatcaata tgattaaagc gatggcggtt gctccacaaa
     4501 tgacggatac atgggataaa cagctggcaa gctatgtttc atcaagtttc cgtgtcaccg
     4561 tattagcaac cattgggcaa caaggtgtac aacttattca aaaaaccgtt atggtgatta
     4621 acctttggtt aggggcacac ttagttattt caggcgatct gagtattggg caattaattg
     4681 cctttaatat gctatcaggg caagtgattg caccggtgat tcggctggct cagctctggc
     4741 aagatttcca acaagttggg atttccgtca ctcgcttagg tgatgtttta aactctccaa
     4801 ccgaacaata tcaaggcaaa ttatcactac cagaaataaa aggcgatatc tcatttaaaa
     4861 atatccgctt tagatataaa ccagatgcac caactatttt aaataatgtg aatttagaaa
     4921 ttaggcaagg agaagtgatt gggattgttg gacgttccgg ttcaggcaaa agtactctga
     4981 ctaaattact gcaacgtttt tatattcctg aaaatgggca ggttttgatt gatggacatg
     5041 atctagcctt agctgatcca aactggctac gccgtcaaat aggtgtagtg ctgcaagata
     5101 atgtgttatt aaaccgcagt atccgagaaa atattgcgct atcagatcca ggaatgccaa
     5161 tggagcgagt aatttatgca gcaaaattag caggggctca cgattttatt tcagaattgc
     5221 gtgaaggtta taacaccatt gtgggtgaac aaggagcggg gctttcaggc gggcaacgcc
     5281 aacggattgc gattgctcga gctttggtaa acaacccgaa aatcctgatt tttgatgagg
     5341 caaccagtgc cctcgattac gaatctgagc atattattat gcaaaatatg caaaaaatat
     5401 gccaaggcag aaccgtgatt ttgattgcac atcgtttatc gaccgtcaaa aatgcggatc
     5461 gaattattgt gatggaaaag ggggaaattg ttgagcaagg caagcaccac gaattactgc
     5521 aaaacagtaa cggactttat tcctacttac accaattaca acttaattaa gaaggaaaac
     5581 aatgaaaata tggcttagtg gtatttatga atttttccta cgctataaaa acatttgggc
     5641 agaagtatgg aaaattcgta aagaattaga ccacccaaac agaaaaaaag acgaaagtga
     5701 atttttaccg gcacatttag aactgattga aaccccggtt tctaaaaaac cacgtctaat
     5761 tgcttatttg attatgctat ttttagttgt ggcaattgtg cttgccagtg taagcaaagt
     5821 tgaaattgtg gcgactgctc ccggtaaatt aacttttagt ggcagaagta aagaaattaa
     5881 accgattgaa aacgccattg tacaagaaat tttcgttaaa gatgggcagt ttgtggaaaa
     5941 agggcaatta ttagtcagct taactgcatt gggttctgat gcagatatca aaaagaccat
     6001 ggcttcactt tctttagcta aactggagaa ctatcgctac caaactttgc ttactgccat
     6061 tgaaaaagag tccttgccgg tgattgattt atctagaacc gaatttaaag attcatcgga
     6121 agaagatcga ctacgtatta aacacttaat tgaggagcaa tacaccactt ggcaaaaaca
     6181 aaaaacacag aaaactttag cgtataagcg taaagaggct gaaaaacaaa caatatttgc
     6241 ctatgtccgt aaatatgaag gtgcaacacg tattgaacaa gaaaaattaa aagactttaa
     6301 ggcactttat aaacagaagt ctttatctaa gcacgaactt cttgcgcaag aaaataaatt
     6361 aattgaggct cagaatgagc tagctgttta tcgctcaaaa ttaaatgaat tagaaaatga
     6421 tctactcaat gtaaaagaag aacttgaatt gatcacgcaa ttctttaaaa gcgatgtgtt
     6481 ggaaaaatta aagcaacata ttgaaaatga acgccaactt cggctcgagt tagaaaaaaa
     6541 taatcaacgc agacaggcct cgatgatcag agcaccggtt tccggtacgg ttcagcaact
     6601 gaaaattcac actataggtg gtgttgttac gactgctgaa accttgatga tcattgtgcc
     6661 ggaagacgat gtgttagagg ccaccgctct ggttccaaac aaagatatcg gctttgttgc
     6721 agcagggcag gaggtgatta ttaaagtgga aactttccct tatacacgct atggttatct
     6781 aactggtcga attaaacata ttagcccgga tgcgattgaa caacctaatg taggcttagt
     6841 ttttaatgca actatagcta tagataggaa gaatctaaca tcgcctgatg ggcgaaaaat
     6901 tgatttgagt tcaggtatga caataactgc tgaaatcaaa accggtgaac ggagtgtaat
     6961 gagttattta ctcagcccat tagaagaatc tgtcacagaa agtttaaggg aacgctaatc
     7021 gaaccaaaac aaagccataa aagccatttt gagcttttat ggctttattt tttagtccac
     7081 aagcggtcaa aaaagcccaa ttttttacac ttttataaca aattgttcta actaaaaatt
     7141 actaattctt ttcttttata agacgaatcc tctattttca tttcattaac attgaactag
     7201 aaaaggggat ttatgagcct aagcattacg aatctttctc ttggctaccg caaaaatcag
     7261 caaaggctta tttgaaaagc acggtgtgca ggtggaaaaa ccggtgatgt ttcgcagctg
     7321 ggctcagttg gtggaagcct ttttaagtgg caatgtgaac gtggtgcatc tgctttcgcc
     7381 tatgagtttg tgggcgaaat atggagcaaa tgctccggtg aaagcggtaa tgtggaatca
     7441 cttggcaggt tcggctttaa cggttcgccc tgaaatcaac agtattgccg aactctccgg
     7501 caaaacggta gcaattccgt tttggtattc aattcataat atcgttttgc aacgattatt
     7561 acgagaaaat ggcttgaaaa tcaccgaaaa agagccgaaa agcggtgaag tgcgattaag
     7621 tgtaatggct ccgtcagata tggtggcagc gttggcaagt gaggcaattt ccggctttat
     7681 tgtggcagag ccgttcaatg cgttagcgga agccaaaggt gtggggaagg tgctgcgttt
     7741 tagtggcgat gtgtggaaag atcacgcctg ctgtttaacc ctaatgcacg aatacgatat
     7801 tcaacaccgc ccggaatggg tgcaaaaagt ggtgaatgtc ttaaccgaag cccaagtgtc
     7861 cacgcttgcc aaccgtgcag aaaccgctca attattggct cggggcagcg gctacacgcc
     7921 gcacgatcaa aaagtgctgg aaaaggtact ggctccaacc caagctcagt gggataaata
     7981 tatcgaaaca ggggcaattc aaaaccctca gtggcaccaa cagcgaatcg gtttccaacc
     8041 gtatccgttc gattcctata tggaagagct ggtcaaaatg ctcaaacaga cccacattgc
     8101 cggcaataat gcgtttttac aacagcttga cccggtagag gcagcgaaag cgattaacga
     8161 gccgaccttt gtgcgaaaag cgattgagca aggcggttgg gttgaaacct ttaatttaca
     8221 aaccggttgg acaagaaccg agcaaattag tgtataagcg ataggaacga acgatgaaac
     8281 agaaatatat ttacggctta gccggtttat tactgctact tttaggttgg cagcttggca
     8341 cagccttgtt aagccaacgg ttgccgatgg cgtcaatgtt agctccggca aaaacctggg
     8401 aaagttttac ttggctgatt tcctccggcg aaatttttcc gcacttatgg gcgagtttgc
     8461 aacgcattgc tgtcgggcta ctcgctgcgt tagtgattgg tgtaccgctg ggcttattgc
     8521 tcggcttctc caagcacctt gaaatgcttt gcacgccgtc attccaacta ctacgtatga
     8581 tttcgccact ttcgtggatg ccgattatcg tgatgttact tggtgtaggc gatttgccga
     8641 tttatttttt actggcgttt gccaccgttt ggtcgttgat tatttccacc acctcagggg
     8701 taaaaagcat tgatccacaa tggttacaac tgggtagagt aatgtcggct acccgcaaag
     8761 aactgctgtg caaaattgtt gtaccggcta ttaccggaca cgttctcacc ggtttacgtg
     8821 tggcggttgg gattgcgtgg gtggtgctgg taccgtgcga aatgctaggc gtgaatcaag
     8881 gactcggcta ttttatttta gacacccgag accgattagc ctattccgaa ctgatggcgg
     8941 taattataat tatcggctta atcggctggg cgttggatag cttaatccgc ttggctttag
     9001 cgaaaggcgg cagataaatt gacaagcggt cgaaaaattg catttttttg caaaaaatcg
     9061 accgcttgtt tattcccaaa aagcatatta ccttaattta ttttatttct agggcataaa
     9121 aaagcgattt aagatagcga caaagtaaac taattatacg aggtaaacac aatgtcggtt
     9181 ctttcatctt cttttattca atggcttcag cagaacgccg aaaatttaga tcaatctaac
     9241 gaacgagcgg atttactcat tccacaaatt gccgcactag ggctatataa aatcggcgtg
     9301 ccggaaacgc ttggcggcag cggtggcact ttggttgatg cgatcaaagc cgttaccgaa
     9361 ctgggtaatc actcgctggc tgcggcattt gtttcgtggg gacatcgtac ctttatcgag
     9421 catattatcg cctcaaaaaa tcctattccc cgccaaagct atttggcgga tctgctaaat
     9481 ggcaatttaa ccgcaggaac aggtttatcc aatgcgatga aatttctctc cggcattgaa
     9541 gagttgaatg tgaatattcg tgaagaaaac ggtaaacgct atttaaatgg ccgcctgcct
     9601 tgggtaacca atttacgttc ataccgcttt gttgctagtt tcgttgccgg ttttcaagac
     9661 gaacgcaaac cgctagtgat tgcaattccc tccaccgcca aaggcttaac ccgcaccaaa
     9721 gaattggaat ttatcgcatt gcaaggcacg aatactacct ctctcgtatt tgagaatgtg
     9781 gaattacaag atgaatggat tttggctgat gacgcgcctg agtttttagc agccactcgc
     9841 cctgcgttct taggtttaca atttggtttg ccctttggct tggttgaacg tagtttggcg
     9901 gaaatcgaaa aatcgctccc tttccgtgca attctcgcct ctgagtggca agctcaagtt
     9961 gatgccctca acgatattaa aactcgtctg tttaatggct taaatgaaga aggttatttt
    10021 gtgcgtaatc cgaaagagct tttccaagcg agaattgaca ttgtcaatgt cgtcgcccaa
    10081 actattctgc tagaactgca agcgagtggc ggacgtggct atctgaaaaa tgatacttca
    10141 ggcttcagcc gccgttggaa agaagcggca tttctgcccg ttgttacgcc aagtgcggta
    10201 cagctgagaa tggtgctggc gaatagttaa gcggataaga gacaaattgt tgcctctttt
    10261 attttgcaaa aaatagtcta aatataaccg cttgcaataa aaataaaaaa ggatgcattt
    10321 tttatgcttc ctcaatttta cggctatcta ttatgcaaaa ggctgcaatt cagggttaat
    10381 tggaatttcg gcaatgttgt taacgtagtt acataatgtt gccaagctca cgcctaaaat
    10441 cacatcaatc gcattttgtt ggttgtaacc tgcggcaaaa aattcgttta attgggcttc
    10501 ggttaatttg gctttttgta acattactgc aagggtgaag cgagccagag tgtctaattt
    10561 gttatcgtct atgcgagccg tggcacgtaa agcatttacc acttcttcag gcatttttaa
    10621 tgcttttaag gcgattttag tatgacctgc cacgcagaaa ccacagccgt tgaccactgc
    10681 tgcggtgatt tgtaccactt cacgttcttc agcggttaag ctgttacgac tattaatacc
    10741 acccacagtg cggtaagttt ccagtgctgt tggtgcgttg gctaatacgc cgatcagatt
    10801 tgggataaaa ccgtttgctt gttgcactgc ttttagggct tcttgtgccg tttcaggagc
    10861 ggattcaata gtgtgaattt gaaattgaga cataaatgtt ccttatcagg ttgtttattg
    10921 gtgtggctca tcataaagaa aaggctaatt tagaacaaat caaaatcact atttaatata
    10981 actaaaagat cttaaaggga gtatagttgc ctatactccc tttgtttatg cacaggttgc
    11041 gtttttactc ttctgcgtct tcttttgccc attctaatga gcggcgaacg gcttttttcc
    11101 aacctttata gcgtttcgcc cgtttttctt ggtcgccgtc cggtgtgaag gtgcgttcaa
    11161 tttccgcttt gtctttcagt tcttccaaat ctttccagaa gccgacagca atacccgcaa
    11221 gataagctgc ccctaaagca gtgacttctt tcactttcgg acgctcaact ttggtgtcta
    11281 aaatatcggc ttggaactgc attaagaagt tgtttgcggt tgcaccaccg tccacacgta
    11341 acgcttgtag tttcgctttg gagtcagact gcattgcttc taacacatct cgggtttggt
    11401 aagcgataga ctctagggtc gcacgcacaa tgtggttacg gtttgcccca cgggttaaac
    11461 cgaaaattga gcctcgtgca tacggatccc aatatggagc acctaagccg gtaaaggcag
    11521 gtacaacata aacaccgtta gagtcatctt ctttagtggc aaaatattcg ctgtcaaagc
    11581 tatcgtgaac aattttcagc tcatcacgca gccattgaat agacgcaccg gcaataaata
    11641 ctgaaccttc caacgcatat tccggctcgc cttttgcgtt acacgcaatg gtcgttaata
    11701 aaccgttttc agattgaatc gcagtgttac cggtgtgcat caacataaaa cagcctgtac
    11761 cataggtatt cttcgcttgt ccttctttca cgcataaatg accgtagaga gcggcttgtt
    11821 ggtcacctgc aatacccgcg accggaatac gcacaccgcc ttgaccgccg atattagttt
    11881 gcccgtaaac ttctgatgaa tttttcactt caggtaacat tgagcgtgga atattgagca
    11941 attccagcat tttatcgtcc cattgtttgg tgtgaatgtt aaacaacata gtacgagaag
    12001 cgttggtgta atcggtaacg tgtacccgcc cttgggttaa tttccacacc agccaagtat
    12061 cgaccgtacc gaacagcaat tcgccacgtt ctgctttttc tcttgcccct tctacgttat
    12121 ctaaaatcca cttcacttta gtgccggaga agtacggatc gactaccaag cctgtcgttt
    12181 tacgaatata ttcctcgtgt ccgtctgctt ttaatttatc ggtaatatcc gatgtgcgac
    12241 gacattgcca cacaatcgca ttataaacag gtttaccggt ctctttttcc cacacaatcg
    12301 tggtttcacg ttggtttgtg atcccgattg cggcaatgct atccggctta ataccggctt
    12361 ttgccaccac ttcatttaag gtggaacttt ggcttgacca aatttccatc gggttatgtt
    12421 ctacccagcc tgcttgcgga taaatctggg taaattcacg ctgagccact tcaaccacat
    12481 tggcattttt atctaataaa accgcacgag agctggttgt accttggtcg agagcgatga
    12541 tatattgttt ttccatagat tactcctgag tatttgctgg aagattgtta tgaatgaggt
    12601 ttttataacc ccaagcaccg aataatgcac cgcagattgg ggcgacaatc ggaatgatga
    12661 aatacggaat ttcttttccg cctgttaaag caatttcacc ccaaccggca agataagcaa
    12721 agagtttcgg accaaaatca cgagccgggt tcatcgcaaa gccggttaac gggccggttg
    12781 ctccaccaat taccgcaatc aataaaccga tgagtaatgc ggctaaggct ttattcggca
    12841 agccgttttt atcatcgcca atcgctaaaa tagtggacat caacaccaca gtaatcacaa
    12901 attcaacgat gaaggcggta accaatgtaa tattagggtt tggataggtg gagaacacac
    12961 cggcaaagcc aacagtttcg ccccgagtaa tgccttgagc ggcttcggcg gcagtaaaga
    13021 gatcgctata cataaaataa atcagtgcga cagagcaaaa cgctccggca acttgggcga
    13081 tgacataggg caaaatttta tgcttttcaa agccgtagaa tgcggcaagt gctatagtga
    13141 ccgcagggtt aaggtgagca ccggaaatac cggcagaaat gtagattgcc atggaaacac
    13201 caagccccca aacgatggaa atttcccata aaccgaaatt cgcaccggct acctgagcag
    13261 cagcaacaca accagcacca aagaaaagaa taaaacctgt accgataaat tcgccaatac
    13321 aggcattacg taaggatttg ctcataaagt ttctccttaa gatgaaaatg attggaaagt
    13381 gatgaaaaaa tgtttgaata tgaacatatt tcggagtgat attgccaaag cggcattttt
    13441 taagcaaata ggaagtttca aatttgtgaa atccatcaca ttatttttct aaatcactca
    13501 tttttaggta tgatgtgaga aaacgttaga tgaggaaaaa tgatgtcttc aatacttatg
    13561 atttcaatcg gggcggtact tggagctatg tgccgctggc aattagccgt ttggctaaac
    13621 ccgatactca gccaattcgc ctttggtact ttagcagcaa attggcttgg ttgtttgtta
    13681 attggtattg caatggggtt taatttaggc gagagccagc ggttgctgtt catcagcggt
    13741 tttttaggca gttttaccac cttttccagt ttctctttag aactaacgga aaaactatta
    13801 gcagaaaaat gggggcagtt tgtgacagtg ctgagtttac atttaatcgg tgggcttttg
    13861 cttactttga taggtgtaat gcttgcaaga gccgttacaa gcggtcgttt ttgctgaaaa
    13921 ttttgcaaat aaaaaacctc ccaaacggga ggttttgcga gcgattaaag taggatattg
    13981 gaaagtaata aaccaaatcc gatactgaaa atcatactaa gtaagcccgg taacataaag
    14041 ctatggttga aaatatattt accgatacgg gttgtgccgg tggtgtcgaa gtcaatcgag
    14101 gcaataatcg ggccgtagtt cggaatgaag aagtagccgt ttaccgccac aaataccccg
    14161 attagcaccg gtgcaggaat gccgatagca atcgctacag ggaataaggt tgcaaccgtt
    14221 gccccttggc tgtttaccaa tacggaaagt acgaataatg cgaaagcaaa tgcccaaggt
    14281 gcggtttcaa ctaagcctga aaccattgat ttcacttcgt ccatatgacc ttgcattaag
    14341 gtatcgccta accaagcaat accgaaaatc gcaatcactg cacgcatacc ggcgtggaag
    14401 acagaacctt tggtgatttc attaccgtcc ggtttgcaga agaagataat caacgcacca
    14461 atacttaaca tcacaatttc aatcgtgtga gccatcccca tcggcttacc atcaaacaca
    14521 gggcgaagag ctggaattgc gcccattaat accactaata atgcaccgaa taggaataag
    14581 cctactgata atttcgcagt cggttttaac ggtaattcgg ttggattggt agtaatgtga
    14641 ttttctttca catattcagg gtcttgcaat aagcgttgat aatttgggtc gtcttttaat
    14701 tctttgccca ttttatttac aaacacacaa gctaaaccta agcccaataa ggtcgcagga
    14761 atagtcacac ttaacacgtt acccaatgta acaccttgag gttcaaggaa ggcaaccaca
    14821 gcaactactg ccgcagcaat cgggctggcg acaatcgcaa attgtgaggc gattactgcc
    14881 attgaaagtg gacgctcagg gcggataccg ttgtggcggc ttacctctgc aattacaggt
    14941 aatacagaat aagcaacgtg acctgtaccg gcaagcacgg tgaatgtcca agttacaagc
    15001 ggtgcgataa aggtaatgta tttcgggttt ttacgcaaaa tgcgagtggc aattttgatc
    15061 atatagtcca aaccaccggc tgcttgcatg gctgctgcag ccgacacaac cgccataatc
    15121 ataaacatta catcaatcgg taaaccggca ggttttagcc caaacccaaa tgaaagaatg
    15181 gctaagccca agccaccgaa cacccctaat ccgataccgc ctacacgggc gccaaccaaa
    15241 atgcatagta acactatggc aaattggata ataaacatcg cagacataat tttttgctcc
    15301 taaataagta aatgaaactc tgtgtttgaa aaaatctgca tttttcatca aattaatcgg
    15361 cacgtactat atcaataaat aaaactaatc tctataatct aaatcaataa aatttctaat
    15421 taattaagga ataatatgca attttcaaaa atgcacggct taggcaatga ttttatggtg
    15481 gttgatggtg taacccaaaa cgtttacctt accgaagaga tgatccgtac gctttctgat
    15541 cgccatcgtg gtatcggttt cgaccaactt ttattggttg aaccgcctta cgaccctgaa
    15601 ttagattttc actatcgcat ttttaatgca gacggcagtg aagtgtcgca atgtggcaat
    15661 ggggcgagat gttttgcccg ttttgttacg ctcaaaggct taaccaacaa aaaagacatt
    15721 cacgttagca ccgcaaaagg caaaatggtg ctaagtttgc aagaagacgg caaagtgcgt
    15781 gtgaatatgg gcgaaccgat ttgggaacct tcgcaagtgc cttttacagc aaataaattt
    15841 gagaaaaact atattttacg caccgagcta caaaccgtgt tatgtggcgt ggtttcaatg
    15901 gggaatccgc actgcgtact gcaagttgat gatgtgaaaa ccgccccggt aaatgaactc
    15961 gggccgttac tagaaagcca cgagcgtttt cctgaacggg cgaatatcag ctttatgcaa
    16021 gtagtgaatc gcaatcacgt taaattacgg gtgtatgagc gtggagcagg cgaaacccaa
    16081 gcctgtggta gcggtgcgtg cggcacagtg gcggtcggca ttatgcaagg cttgttagat
    16141 aacaaagtgc aggtggattt acccggtgga acgctgatta ttgaatggca aggagtaggg
    16201 catcctctgt atatgaccgg cgatgccacc cacatttatg atggatttat taaactgtaa
    16261 gttttcccat agggaggcaa gcggtttatt tttaccgttt ttttgcaaaa tagggttggt
    16321 ttatacaaaa gtaccagcgt ggacgctggc actatcagac tgatgtataa cttttgttta
    16381 aattcaactt atagatggtg caagcgtcct cgcttgtacc aaaacaggtt tcactcgagt
    16441 aacttcttca ccaactccaa caccacaggt aattcggtaa caccttctct atccaaaaaa
    16501 tgcccgcctt gatttaaccg aatataatcc gcgtgcaaat gttgggctaa cgcatcactg
    16561 tattggtgat tcacgacctt atcatctaac gcacttacca catagcttgg aaagctcgat
    16621 acgctcggca aaatcgcata atagttggta aaggcagaca gttcgggtaa gttttctacc
    16681 ggttgataga accccgacac aaacaccgcc cctttaatct tggcgtaatt tttggctaaa
    16741 aaattcaaaa tggcaatgca acctaaacta tgcccgacaa aaaccgtttg atcggtcagc
    16801 tccacatttt gtgcaagatg atttaaccaa ccatctaatg acggctttgc cgaatccggc
    16861 atattcaacc gctcacaact gacaccacac ttcgctaatt cttgctccag ccaaggaaac
    16921 caatgcttat cggcattcgc cgtataaccg tgtacgatat aaacctgtgc catttcacac
    16981 ctcctgattc accaaagatt gtaccatagc ttaatcagga aaaaacaagc ggtgattttt
    17041 ttaaattttt ttgcaactca ggtaaaatag cacgcattta cgcaaagagg tcatcaatga
    17101 acgtttggat tttacttgcg atttccatct gcctcgaaat cgctgccacc aatttactca
    17161 aattaagcaa cggcttcacc aaagctatac cgactattgg ctcgctcgcc ttatacggtt
    17221 tatcctttta ctttctctct attattttcc gcaccctacc ggtcggaatc gtgtatgcta
    17281 tttggtccgg cgtagggatt gtccttaccg cccttgttgc ttattttgct ttcggacaga
    17341 aaattgactt ggcaggctta atcggcatca gcctcattat tgccggtgta ttggtgatta
    17401 atttgttttc taaagtgtga tacaagcggt tgattttaca aaaaaatctg caaaatcaac
    17461 cgcttgtaat agttggcaag aattataacc cctttaaccg ccggtaaagc tcagtaaatt
    17521 tttggcgttt ttgttgatat tctgcatagc gggcaggggt tggttggtaa acctgttcca
    17581 ggggtaacgg ctgacagaac gtagcaatat cctgatttgg gtttagcgca atttgagcga
    17641 gttttgccgc ccctaatgcc ggaccgacat caccactggt gcggtattca aaggttttac
    17701 cgctaatatc ggcaagtagt tgtcgccagt acacactttt tgctccaccg ccaattaatg
    17761 caatattgtc agcaactaca ccagtttcgt ggagtacctc aatcccttca gccaaagcga
    17821 aacttacccc ttcaatcacg gcttttgcca tcgttatttg gttgtcatta tggcttaatc
    17881 cccaaaatac gcctttcgca taagggtcat tatgcggtgt acgttcaccc gataaataag
    17941 gtaagaaaat agcatcagat gccgtttggc ttgcttcaac ctgttggaat aaagtcgata
    18001 catcttttag tcccaatgat tttactgccc aatctaccgc agaagcggca cttaatatta
    18061 ctgacatcag atgccaacga tcaggtaaag cgtggcaaaa actatgcact gctttttgcg
    18121 gattagcctg aaatttatcg ctcactacaa aataaacccc tgaagtcccc aatgagagca
    18181 tagcttgccc ggtttggtat aagccaatgc cgattgcacc ggcagcatta tcgccaccgc
    18241 ctgccacaat cggcacagat ttcattttcc attgctcggc aagggttgga cgtaaataac
    18301 ctgtgatttg attgccttca aacagtttcg gcatattttc aatgtctaat ccgcaagcat
    18361 ttagtagcga tttgttccaa ttccgtttac ccacatcaag ccacattgtg ccggaggcat
    18421 cagacatatc agaggcatat tcgccgctca tcaacaagcg gagataatct ttcggtagca
    18481 agactttgct gactttttcg gcaatatcgg gctggtgttt atcaacccaa cgcaatttcg
    18541 gagcggtaaa gcccggcatc atcaggttgc ctgtaatttc acggctattt ggcacaagct
    18601 gttctaactc ttcgcattcc ataaaactac gtccgtcatt ccataaaatc gcaggggaaa
    18661 gtacattatc tgcgttatct agtaaagtcg ctccgtgcat ttgcccggtt aaaccaatcg
    18721 cttttactgc ttgtaaatct tgagtagcgg aaagttctaa catcgcttca ttggtggcat
    18781 tccaccaatc ttgcggattt tgttcagacc acaatgggtg tggacgaaaa ataggaagtg
    18841 atttttgtgt gatggcaata atgtgttgat tttcatcgag taacacgact ttcacacccg
    18901 atgtgccgag atcgattcca atgtacataa atattccttt gttattgaat acaagcggtc
    18961 attttattcg gaacatttgc aattttaagt tttggagatc tatcacccct ctttagcaaa
    19021 gagggggatc agatcgagct taactttgca aaaaattgat aaaaaacaac cgcttgtaga
    19081 ttaacggtaa atatagctat ttaccaaatt ttccaaatat tcttgctgac cggaaacagg
    19141 ttttggatct aacccttggg tttccactat ttttgccaaa tcttcaaggc ttgctttacc
    19201 gtttagaatt tgtaagccca aatcttggtt ccagccagcg tagcggttat ctactacttt
    19261 ctgtaatgct tgatcttcca gcatttttgc cgcacgtttt agcgaaaggg ctaacacatc
    19321 aattgccccg atatgtgcgt ggaataaatc atacggatcg gtactttggc ggcggatttt
    19381 cgcatcaaag ttgaaaccgc ccgttgtgaa accacctgct tttaagattt catacatcac
    19441 aagggtgttt tcttccacgc tgtttgggaa ttggtcggta tcccaaccta attgcggatc
    19501 acctcggttg gcatcaatcg agccaaaaat atctaatgcg gtggcagtcg caatttcgtg
    19561 ttggaaagta tgccctgcaa gagtggcgtg gtttgcttca atgttgactt tgatctcttt
    19621 ttctagacca aactgtttta agaaaccgta aacggtggca acatcgtagt catattgatg
    19681 tttggttggt tcttgtggtt ttggctcaat taataaagtg ccgttaaaac cgattttgtg
    19741 tttatgttcc accactaact gcataaagcg accgatttgt tcacgctcac gttttaagtc
    19801 agtgttgagt agggtttcat aaccttcacg tccgccccat aatacataat tttcgccgcc
    19861 taagcgtttg gttgcgttca ttgcggtaaa gacttgtgct gccgcccaag caaagacttc
    19921 cggattcgga ttggttgaag cacccgacat atagcgagga ttggtaaagc agttcgccgt
    19981 accccataat aatttcacgc ctgtttcagc ttgtttttgc tctaaaatat ccacaatagt
    20041 gttgaaattg tgctgatatt ctttgaaatt gttgccttcc ggtgcaacat ccacatcgtg
    20101 gaagcagtag taaggaatgc ctaatttttg gaaaaattca aaggcaattt ccgctttttg
    20161 cttcgcacct tgcagtaaat cccccgtttt ttgccagctt ctgtcaagcg aacccacgcc
    20221 aaacatatcg ttacccgtcc agcagaaggt gtgccaatag caaaccgcta accgcagatg
    20281 ttctgccatc gttttgccta aaaccacttc atccgggttg taatgtttga atgcaaacgg
    20341 gttggtggaa ttagcccctt catattgtac ttttgagatt ttgtcgaaat agttagacat
    20401 aataatgctc ctatgaattg gaaaaataat ctttcggtaa catcatcgga aaattatgca
    20461 aaaccttcaa ttacgatatt tcgtttttgg atttcgatta ttggt
//
LOCUS       DANLXF010000010        20433 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.010, whole genome shotgun sequence.
ACCESSION   DANLXF010000010 DANLXF010000000
VERSION     DANLXF010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 20433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20433
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.010"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            109..867
                     /locus_tag="PXD66_000222"
     CDS             109..867
                     /locus_tag="PXD66_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03761 family integrating conjugative element
                     protein"
                     /protein_id="HDL6318561.1"
                     /translation="MTTPLDLQLGPLRSEITFTLHTQYAHKLWMGRPMIRNEEGKVTQ
                     SSIISVPNCFAMLTQIQRAASEDDPYADDYLIQFEESVINYRKEIQKLTNDIVTLYAK
                     MLPEGISIERCANISPISYPIYVNSQLGYQLLYLLGDFDSLCRSVMTAAHIAIINRAE
                     AQDWIEAGARLIRKCFGIVERYKNSGITRRDYQENNARYQAAVKRMGYTLSDAVLTGE
                     HRAEFAPFIKQNGIVEEEQPVETHITTQANESQE"
     gene            876..1385
                     /locus_tag="PXD66_000223"
     CDS             876..1385
                     /locus_tag="PXD66_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3158 family protein"
                     /protein_id="HDL6318562.1"
                     /translation="MNLISDEIYRQLVKDSGLNASLKQLFSHFDTVSDYAVLQEQIQQ
                     ARAYFMNAQDGMVQYVRSEFSPLAVYMIKDKASSSGGTFLRWRSMQNARTGGTVWQPI
                     VDDESVPVDVRQKVVAVEKDRILINMQISVFNHILRQLVDCTEKLKEVDNAVAKSGLY
                     PLKNPVCNP"
     gene            1491..1691
                     /locus_tag="PXD66_000224"
     CDS             1491..1691
                     /locus_tag="PXD66_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318563.1"
                     /translation="MANVKMKFSVEIDGEVIIQHEIEEQAEKRSYFSQERKLAKEIIN
                     KMNEFYSDRWIDAKPWIASDDK"
     gene            complement(1807..2010)
                     /locus_tag="PXD66_000225"
     CDS             complement(1807..2010)
                     /locus_tag="PXD66_000225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318564.1"
                     /translation="MELRKFPHHIIFIEQAKYGLQNYLLFELLTRTGQAVYFKHPIFH
                     TPNKEMRIVFVQDHKFLSLWQNK"
     gene            2119..2637
                     /locus_tag="PXD66_000226"
     CDS             2119..2637
                     /locus_tag="PXD66_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
                     /protein_id="HDL6318565.1"
                     /translation="MRYIKYSLLLISTMIMSGCAELSAINDKVGDWAGQMNQTLYGSK
                     ESSVEQHNGRQVYIKTRNGKYVYQDSGTLQLKQNIDEVFLKVRREFGFRPTNADSDAR
                     KVYHTVQGTFYHVSGYFKSDVDNDGVDLGENYLEVIIEKGSKGTVNVEWKATGSTRWL
                     DSTKQRLLNTLK"
     gene            2798..4849
                     /locus_tag="PXD66_000227"
     CDS             2798..4849
                     /locus_tag="PXD66_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL6318566.1"
                     /translation="MKLFICEKPSQARDLASVLGATSKGDGLLATNDKSIIVTWGFGH
                     LVEQYQPEDYDEVWKRWAFETLPIIPAQWKMAPKKESKKQYNVVIGLIKKASLVVIAT
                     DADREGEMIARELLDLAGYRGQIQRCWLSALDDASIRKALQTLKSGKETEALYYAGMG
                     RSRSDWLIGMNFSRLFTLMAQAKGYSGKPLSVGRVQSPTLALVVNRDREIANFVPKAH
                     YSLAVQLATASDETFVAGLSIPEQYLDESGLCLDSRVIQQAEAQIKQVGIAKVITVET
                     KREKKAPPLLYALSDLQGECNRLFGFGAQQVLDIAQSLYEEHKITTYPRTDCGYLPES
                     QLTEVPMVIRSLVAADSELQTLLPRLNLQQKSRAWDDKKITAHHGIIPTIKKADLSKL
                     SEEEMKVYDLIRRRYLAQFLPHLETDKTIATLQSSGHTLIARGNVIVSQGWRILFGKN
                     ADEDVGSDDEKQGLPALTANQQCRVTHSEVRTLQTKPPAHYTEGTLLEAMKNAARFVT
                     DPRLKQRLRETEGLGTEATRAGLIQGLIDKGFLIKKKKSLMASAEAIALIDSLPDLLK
                     NPGLTALWEQALNQIAEGSMTLDDFMQRQETFVRQLIGNCMQQGMSLGNIEIRKCPEC
                     GKPMRKINHAKGTFWGCTGYPDCQHKEADKKVKSTSNKSTKKNSSLNVLDQLNKLRSQ
                     L"
     gene            5670..6101
                     /locus_tag="PXD66_000228"
     CDS             5670..6101
                     /locus_tag="PXD66_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3577 domain-containing protein"
                     /protein_id="HDL6318567.1"
                     /translation="MTTQTSTQKNYFNLHTTGIGYLNDIRLVEPKKGNPFYACRIAAL
                     VGESDSPEYRYFDMNVVGKETETLIKRCQKAVSEEKKVLVSFVMADLWFDTFTYSTDS
                     KFHKKGDTGVSVKGRLIRIKMIKIDGEIAYQEKPKSDEQSE"
     gene            6119..7111
                     /locus_tag="PXD66_000229"
     CDS             6119..7111
                     /locus_tag="PXD66_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL6318568.1"
                     /translation="MLCWALLLDVQDLRVTFKTPDGDVTAVNDLTFSLNAGETLGIVG
                     ESGSGKSQTAFALMGLLADNGRISGSAKFNGQEILNLPETQLNRLRAEQIAMIFQDPM
                     TSLNPYMKIGEQLMEVLMLHKGMDHKTAFEESVRMLDAVKMPESRKRMNMYPHEFSGG
                     MRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKSEFNTAIIMITHDLG
                     VVAGICDNVLVMYVGRTMEYGTAEQIFYTPSHPYSIGLIGAVPRVDGQEEELITIPGN
                     PPNLLRLPSGCPFQPRCVHATKQCLKAPALTLLNDQRLRACFVPAEQLIRGNAS"
     gene            7108..8097
                     /gene="oppF"
                     /locus_tag="PXD66_000230"
     CDS             7108..8097
                     /gene="oppF"
                     /locus_tag="PXD66_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HDL6318569.1"
                     /translation="MNTEKKVILEVKDLSVHFNIKDTKSLFFAKHQTLKAVNHVSFQL
                     YEGETLGVVGESGCGKSTLARAIIGLVESSGGNILWLGKELTQQSAKEWKTIRSDIQM
                     IFQDPLASLNPRMTIGSIIAEPLKIYQPHLTAEEVKEKVRAMMMKVGLLPNLINRYPH
                     EFSGGQCQRIGIARALIVEPKLIICDEPVSALDVSIQAQVVNLLKSLQREMGLSLIFI
                     AHDLAVVKHISDRVLVMYLGNAVELGDYEQVYHDTKHPYTKALMSAVPIPDPQLERNK
                     QIQLLEGDLPSPINPPSGCVFRTRCPLADQHCIADKPLLKGRDNHVVACFKAE"
     gene            complement(8201..8959)
                     /locus_tag="PXD66_000231"
                     /pseudo
     CDS             complement(8201..8959)
                     /locus_tag="PXD66_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326384.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
     gene            complement(9089..9730)
                     /gene="bioD"
                     /locus_tag="PXD66_000232"
     CDS             complement(9089..9730)
                     /gene="bioD"
                     /locus_tag="PXD66_000232"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005724996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HDL6318570.1"
                     /translation="MAKVFFITGIDTDIGKTIATGWYAKKLMQQGASVITQKMIQTGC
                     RGIAEDLLIHRKIQGIELTEEDKQGITCPYVFDYPCSPHLAAKLEPRTIERKKIETCT
                     ALLCEKYDYVLLEGAGGLCVPYNEEETTLDYLCQHQYPVILVTSGKLGSINHTLLSLQ
                     VLNSKRVSVHAVIYNLYPETDQVISQETQHFLRRYLEKYSPNTLFEIMDLISV"
     gene            complement(9748..10503)
                     /gene="bioC"
                     /locus_tag="PXD66_000233"
     CDS             complement(9748..10503)
                     /gene="bioC"
                     /locus_tag="PXD66_000233"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005755527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HDL6318571.1"
                     /translation="MDRIDKGRIKQRFSAAVSNYDQQARAQQQIHQRLLSLLSYIDRQ
                     AFQRALEIGCGTGGLTKQLMSQLQVEHWELNDLCDMREHLQSVLPQPFHFHCGDAEQL
                     PFPQQYDLIASASVVQWFEDKAGFIARCKVRLKKHGFLLFSTFTPENLTQIKHLTGVG
                     LTYPRLEDWQKWLVPEFEILALEHEEIVLHFDDPLAVLKHLKQTGVTATQQQRWTKGQ
                     LHAFCQQYQQHYRNAQHKVELTYTPMYVLARLT"
     gene            complement(10507..11154)
                     /locus_tag="PXD66_000234"
     CDS             complement(10507..11154)
                     /locus_tag="PXD66_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005758126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF452 family protein"
                     /protein_id="HDL6318572.1"
                     /translation="MKTQHISQQGDNLIVYFAGWGTPPSVVEHLILPENYDLCLCYDY
                     RDLHCDVDFSAYQHIRVVAWSMGVWVAERVMQGYPLLSATAINGTGLPCDDQYGIPEA
                     IFAGTLSGLNEDNRTKFERRICGSKTLLRDYQHYSMRPTLQEIHAELTALYTFLQQDH
                     RTDLISWSKAIVGSKDKIFPAQNQRAYWQDRCAITEIAGEHLLFPHFTHWETCWL"
     gene            complement(11151..12314)
                     /gene="bioF"
                     /locus_tag="PXD66_000235"
     CDS             complement(11151..12314)
                     /gene="bioF"
                     /locus_tag="PXD66_000235"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005719374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HDL6318573.1"
                     /translation="MIRYYQQQLADLKAQNQFRQLPQLIHRGRFIQREDNTMLNMSSN
                     DYLGLANNEALRQAFFTQYQDQLPALTSSSSRLLTGSFPIYDELESLMAQAFGRETAL
                     LFNSGYHANIGILPALADKKTLIVADKLVHASMIDGIRLAQCEFVRFRHHDYVHLEQI
                     LQKNDRTFERIIVVTESVFSMDGDCADLTQLVALKQRYPQVMLYVDEAHAIGVLGEKG
                     LGLAEQQGCINQIDILVGTFGKALGSMGAYVICDQVIRDYLVNKMRPLIFSTALPPFN
                     VAWTHFVFQQLPHLQAERAHLAQLSQHLRQAIVDIFQVPMPSESCIVPYILGDNELTV
                     RTAQRLQQQGYYCLPIRPPTVPKGTSRIRFSLTADMQVAEVEQFIACLRELAE"
     gene            complement(12311..13612)
                     /locus_tag="PXD66_000236"
     CDS             complement(12311..13612)
                     /locus_tag="PXD66_000236"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016533607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HDL6318574.1"
                     /translation="MNEQQLLECDRQHIWHPYAALNADFPLFAVERAEGVYIQLKNGQ
                     QLIDGMSSWWSAIHGYNHPRLNHAATQQLSQMSHVMFGGLTHQPAVELAQQLVNILPE
                     SLDKIFFADSGSVAVEVAMKMAVQYQHAKGETQRYKFATIRSGYHGDTWHAMSVCDPV
                     TGMHSLFAKGLPVQYFLPQPPVKFGQAWQPEAMQPLQDLLAQHGDEIAGLILEPVVQG
                     AGGMYFYSPEYLVQAKALCEPYGILLIFDEIATGFGRTGKLFACEHANVVPDVMCIGK
                     ALTGGYLTLSATITNQAVADVICSGEAKCFMHGPTFMANPLACAVAAESIKLLLASPW
                     QQRIQQIEQQLRQELRQAEALDCVAEMRVLGAIGVIEMKAPVNMASLPPRFVQNGVWV
                     RPFGKLVYLMPPYIISSDQLSQLIHGTIRTLQEEYAHKESL"
     gene            13754..14641
                     /gene="psd"
                     /locus_tag="PXD66_000237"
     CDS             13754..14641
                     /gene="psd"
                     /locus_tag="PXD66_000237"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005755522.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HDL6318575.1"
                     /translation="MNILEKKAQLSYWQRIKIAFQYVMPQLYLTRLAGWFAKQQWGAV
                     THFVIKLFAKKYHVDMSEAAKPNFSDYASFNEFFIRPLADNARPINQNPTALCLPADG
                     RISQLGHIEQDLLLQAKGHYFSLNDLLAGDEALAHHFKDGEFATTYLSPRDYHRVHMP
                     CDATLCKMIYVPGDLFSVNPFLAEHVPNLFARNERVICVFDTEFGKMVQILVGATITA
                     SMSTVWAGVINPPRPEKITTWTYEGESAVKLLKGQEMGAFQLGSTVINLFEKDRVQLA
                     SHLQVDSPVRMGEILAHQK"
     gene            14666..15220
                     /locus_tag="PXD66_000238"
     CDS             14666..15220
                     /locus_tag="PXD66_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL6318576.1"
                     /translation="MSQQFFDQVSLESQLEVSAEAVAKGIFDVLNQNPPALELNEVSH
                     ALQTLQQQAKFKEIEAVGAQFLAENAQKAGVNTTESGLQYEVLVAGEGQIPAREDKVR
                     VHYTGTLIDGTVFDSSVKRGQPAEFPVNGVIAGWIEALSMMPVGSKWRLTIPHNLAYG
                     ERGAGASIPPFSTLVFEVELLAIL"
     gene            complement(15280..15603)
                     /locus_tag="PXD66_000239"
     CDS             complement(15280..15603)
                     /locus_tag="PXD66_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005755520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318577.1"
                     /translation="MNWKAVLIFSVLGSLVSCASYSEHAVQRIEAGKSFAVTGNTKRI
                     NTMACQDNDDWYLDGYRVGKSFREHQQKMLIQRTAYCEEQTGKAVPDKFRHSWNSGYQ
                     QGLKR"
     gene            complement(15627..16730)
                     /gene="proB"
                     /locus_tag="PXD66_000240"
     CDS             complement(15627..16730)
                     /gene="proB"
                     /locus_tag="PXD66_000240"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005719361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDL6318578.1"
                     /translation="MKIDKTLVVKFGTSTLTQGSAKLNLPHMIDIVRQLAQLHQAGFR
                     LVIVTSGAIAAGRHYLNHPQLPPTIASKQLLAAVGQSQLIQTWEKLFAIYDIHIGQIL
                     LTRADIEDRERFLNARDTLRALLDNQIIPVINENDAVATSEIKVGDNDNLSALVAILV
                     QAEQLYLLTDQQGLFDRDPRKHPDAKLIADVDKITDHIRAIAGGSGTSLGTGGMSTKI
                     SAADVATRSGIETIIAPGNRPNVIVDLAYGQAIGTKFSVQADRLESRKQWLYAAPSAG
                     IITIDAGAENAMLIQHKSLLPAGIVNVEGRFSRGEVVKIRTQQGKDVALGMPRYNSDA
                     LHLIQGKHSQDIEQVLGYEYGAVAVHRDDMIVL"
     gene            16896..17884
                     /locus_tag="PXD66_000241"
                     /pseudo
     CDS             16896..17884
                     /locus_tag="PXD66_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252470.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
     gene            17959..18606
                     /locus_tag="PXD66_000242"
     CDS             17959..18606
                     /locus_tag="PXD66_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HDL6318579.1"
                     /translation="MTINTYAKFAPTVFVAKCSESHTKGEIITLTSKYGNETEVQVHN
                     LVKQDGEHYFYSFTRCDGMDSQSFAQQKAERYQGYADNAAKRSQDWVNAANEGCDFLS
                     LGEPIKIGHHSEKRHRNLIERNARRMDNAVAEMNKAASYESRIAYWEKMAGKIDLSMP
                     ESLEYFKFELDKAKTKHKELKDNPEKREHGYSLTYAKKAVNELEKKVKLAELLWA"
     gene            18630..19037
                     /locus_tag="PXD66_000243"
     CDS             18630..19037
                     /locus_tag="PXD66_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization protein"
                     /protein_id="HDL6318580.1"
                     /translation="MNRQGIYINGKQVFDSQGEDIQSIGFFGCNIQTVVGINKGSILV
                     SSNSNQLLVNGQSIELGSEPVLKIEVLGNVQKLSAGAADVSITGDAGSVDTVSGDVSI
                     KGNVASYVKTVSGDVRCRAITGSVETVSGDIYR"
     gene            19135..19485
                     /locus_tag="PXD66_000244"
     CDS             19135..19485
                     /locus_tag="PXD66_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318581.1"
                     /translation="MTTINLSQQERELVEFVWNNRNTPWFFHCSVSNVSRNNTSRQIA
                     VCVIKDNQLISLDELIAKLCGYKLRQNGLYVTVHNMDAVFDTLYRFMCKLNDEMEANE
                     NVCPYLSLAHYEVF"
     gene            19576..19938
                     /locus_tag="PXD66_000245"
     CDS             19576..19938
                     /locus_tag="PXD66_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318582.1"
                     /translation="MIDTFFDLDDTSCYLGNWEDILKEYDDCLSVEDFWHSAKEFDTV
                     PHFGNLYQERVILKLIARFCIELNIEQDSGLVEFDYYINAIDTHFYINSQRICDIDDW
                     NEMLDKIREEMTPAKLVA"
     gene            20051..20311
                     /locus_tag="PXD66_000246"
     CDS             20051..20311
                     /locus_tag="PXD66_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318583.1"
                     /translation="MTFELGRVCATRAVAECFNQHTLVDLLHRYCSGDWGNLCDNDKK
                     ANVKAIERGGRILARYDVEDETLYVITEADRSYTTILFPYEY"
BASE COUNT         6086 a         4257 c         4202 g         5888 t
ORIGIN      
        1 tgattaaaaa ataaacagag tagacagtgt ctacaaaggt taaaaaaata gacaatggaa
       61 agcccctttc caaaggctaa aaaacaatca aataaacaag gagcatatat gaccacacca
      121 ttagacttac aattaggtcc acttcgtagt gagatcacgt ttacgctaca tacccaatat
      181 gcccataaat tatggatggg gagaccaatg attcgtaatg aggaaggcaa agtaactcaa
      241 tcctccatta tcagcgtacc aaattgcttt gcaatgctaa cccaaattca acgggctgca
      301 tcagaagatg atccttatgc tgatgattac cttattcaat tcgaggaaag tgtgatcaat
      361 tatcggaagg aaattcaaaa actcaccaac gatattgtca cgctctacgc caaaatgttg
      421 ccggagggaa tttctattga acgctgtgcc aatatttccc cgatttctta cccgatttat
      481 gttaattcac agttaggcta tcagttgctt tacttacttg gtgattttga cagtctttgt
      541 cgctcagtaa tgacagctgc tcatattgcc attatcaatc gtgcagaagc tcaagattgg
      601 attgaagcag gtgcaagatt aattcgtaaa tgttttggga ttgttgaacg ttataaaaat
      661 tccggtatta ctcgccgaga ctaccaagaa aataatgctc gctatcaagc tgctgtaaaa
      721 cgaatgggct atacattgtc agatgctgta cttaccggtg agcatcgtgc tgaatttgct
      781 cctttcatca agcaaaacgg cattgttgaa gaagaacaac ccgtagaaac ccatatcacg
      841 acacaagcaa atgaatctca agaataagga gtgatatgaa cctcatttct gatgaaattt
      901 atcggcaatt agtcaaagac tccgggttaa atgcatctct gaaacagcta tttagccatt
      961 ttgatactgt ctcagactat gctgtattgc aggagcaaat acagcaagca cgggcttatt
     1021 ttatgaatgc acaggatggt atggttcagt atgttcgtag cgaattctct ccccttgcgg
     1081 tctatatgat taaagataag gcttcttctt ccggtggtac gtttttacgc tggcgcagta
     1141 tgcagaatgc cagaaccggt ggcacagttt ggcaaccgat tgtagatgat gaatcagtcc
     1201 cggtcgatgt gagacaaaaa gttgtcgcag tggaaaaaga cagaattttg atcaatatgc
     1261 agatttccgt gtttaaccat attttgcgac agttggttga ttgtactgaa aaactaaaag
     1321 aagtggataa tgcggttgca aaatcaggtt tgtatcccct gaaaaatcca gtttgtaatc
     1381 cctgaaaaag taaggtttgt aaagcctgca ttaaaaggcc ttttaaaggt aaggggacaa
     1441 gcccccttac ctttcacgaa gaaaatgcct ttattatttc ggagtaaaca atggcaaatg
     1501 taaaaatgaa attcagtgtt gaaatagatg gtgaagttat cattcaacat gagattgaag
     1561 agcaagccga aaaacgctct tacttttcgc aagagcgtaa attggccaaa gagattataa
     1621 ataaaatgaa tgaattttat tctgacagat ggattgatgc aaaaccttgg attgcttctg
     1681 acgataaata aaagcaatga gcatcatcga ttacaggttt cataaattga tttcgctaag
     1741 gtatttaact taatagcaat aaaatcttcc tgagtgtagg acgatttatc ccattcttta
     1801 ataaggttat ttgttttgcc atagggataa aaatttatga tcttgaacaa agacaattct
     1861 catctctttg ttaggggtat ggaatatcgg atgcttaaag taaaccgctt gtcctgttct
     1921 tgttaatagt tcaaagagta agtaattctg taatccgtat ttagcttgct caataaagat
     1981 aatgtggtga ggaaattttc ttaattccat aatagtccct agcattttct ctcttactac
     2041 ctaaaagaat tttaagtaga ttttaaccgc acttttgcaa aaaatgaatg cagataaccc
     2101 gcttgtaagg agaattccat gcgttacatc aaatatagct tacttctcat ttctactatg
     2161 attatgtcag gttgtgccga actttctgca attaatgata aggttggaga ttgggcagga
     2221 cagatgaacc agactctcta tggttctaaa gaaagctcag ttgaacagca taatgggcga
     2281 caggtttata ttaaaactcg aaatggcaag tatgtctatc aagattccgg cacattacag
     2341 ttgaaacaga atattgatga agtcttccta aaagttcgcc gagaatttgg cttccgtcca
     2401 accaatgcag atagcgatgc acgtaaggtt tatcatacgg tacaaggtac cttttaccac
     2461 gtttccggtt atttcaaatc tgatgtagat aacgacggcg tggatttagg tgaaaactac
     2521 ttagaggtta tcattgaaaa aggcagtaaa ggcacggtga atgttgaatg gaaagccaca
     2581 ggtagtacaa gatggctaga tagtaccaaa caacgtttac ttaatacatt aaaataagaa
     2641 ctcatatcgc tcctagaacc tcttcccagt ggaaacactg ggattttttt atcgttctca
     2701 tctctcaatt tattgcattc tacaagataa taagtcgttg aatgctattt aatcagcaca
     2761 ttactattgc ctatccgtat tgattttgga aaatagtatg aaactgttta tttgtgaaaa
     2821 gccctctcaa gcccgagatc ttgcaagtgt attaggtgct acatcgaaag gggatggact
     2881 attggcaact aatgataagt ctattattgt aacttgggga tttggtcatc ttgttgagca
     2941 ataccaaccc gaggattatg atgaagtttg gaaacgctgg gcatttgaaa cattgccgat
     3001 cattcctgct caatggaaaa tggctcctaa aaaagagtcc aagaaacaat acaatgtagt
     3061 catcggatta attaagaaag cctctttggt tgtgattgca accgatgcag acagagaagg
     3121 tgaaatgatt gctcgtgaac tgttagattt agccggatac cgaggacaaa tacagcgttg
     3181 ctggctatca gctctcgatg atgccagtat ccgaaaagct ctccaaacac tcaaatcagg
     3241 taaagaaacc gaggcacttt actacgctgg tatgggacgt agccgctcgg actggctgat
     3301 tggaatgaac ttctcccgtc tatttacctt aatggcccaa gcaaaaggct attccggtaa
     3361 gccattaagc gttggtcgag tgcaaagccc aacactagct cttgtggtca atcgagacag
     3421 agagattgca aatttcgtgc ctaaagctca ttactcctta gcagttcaat tagcaacagc
     3481 gtctgatgaa acctttgttg ccgggctttc aatacctgaa cagtatttag atgaatcagg
     3541 gctttgcctt gatagtcggg tcatacaaca agctgaagct caaattaaac aagtaggcat
     3601 agccaaagtg ataacggttg aaaccaaacg agagaaaaag gcaccgccgt tgctttatgc
     3661 attaagtgat ttacaagggg aatgtaaccg cttgtttggt tttggggctc aacaagtact
     3721 tgatattgct caatcacttt atgaggagca taaaattact acttatcccc gtaccgattg
     3781 cggctatcta cctgaatctc agcttactga agtaccgatg gtaattcgtt cattagtcgc
     3841 cgctgattca gagttacaaa ctctattgcc tcgattaaat ctacaacaaa aaagtcgggc
     3901 ttgggatgat aaaaaaatta ctgctcacca cggtattatc cccactatca aaaaagcaga
     3961 tttaagcaag ttaagtgagg aggagatgaa ggtctatgat ctcattcgcc gtcgctatct
     4021 cgctcaattc ttaccgcact tagaaacaga taaaactata gccaccttgc agtcctctgg
     4081 acacactctg attgctagag gaaatgtgat cgtttctcaa ggttggcgta tcctgtttgg
     4141 taaaaatgca gatgaagatg ttggtagtga tgatgagaaa caaggcttac ctgcattgac
     4201 ggcaaatcag caatgccgtg ttacacattc agaagtccgg acattacaaa ccaaaccacc
     4261 ggcacactat actgaaggaa cactactcga agcaatgaaa aatgcagctc gctttgtgac
     4321 tgatccaaga cttaaacaac ggttgcgtga aacggaggga ttgggtacag aggcaactcg
     4381 tgccggattg attcaagggc tcattgacaa aggtttttta attaagaaga aaaaatccct
     4441 aatggcttct gctgaagcga ttgcccttat tgatagccta ccggatttat taaaaaatcc
     4501 cggtttaacg gctctttggg aacaggcact taatcaaatt gctgaaggca gtatgaccct
     4561 tgatgacttt atgcaacgac aagaaacctt tgtacgacaa cttatcggaa attgtatgca
     4621 acaaggtatg tcattgggta atattgaaat tcgaaaatgc ccggagtgtg gtaagccaat
     4681 gcggaaaatc aatcacgcta aaggtacatt ctggggttgt acaggatatc cggattgcca
     4741 acataaagaa gcagataaaa aagtcaaatc gacttcaaat aaatctacca agaaaaatag
     4801 tagcttaaat gtgctagatc agctgaataa attaagaagc cagttataaa tctaagcaga
     4861 ttatggtatt atcaaagtgc aaagaacccg tttaatagat tgtcggttat caatctatac
     4921 cagccgaaat gtctcccagc attctgaaag ggaactctgt aaaattacag gaatgccacc
     4981 taaacaataa atggataatg ccagccactt tatccgttgt attttgtcac atgcgtgatt
     5041 gcaatgtcat aatattaccc gacttgggga tattatgaca ttgcaatcat tgctcacttt
     5101 tcttagcctg agcccacagg tgaataaagg gtgccgtttg tgtcctcgat gatgccacag
     5161 atatgccgat taaagaccac aaggtaatcg ggtaaattac gaacgaaaat atcaacattg
     5221 tcacgaaggt gctgctattt tcaatatagt aacttatatt ggtctaactt aaatttcatt
     5281 tattcttcaa aagtacaaca ccgttggtaa gtgatttatc aacggtgttg tcatttccaa
     5341 ttctctaaaa tcaaaattca attctcaatt aactttatga ttcaatatga ctaaattatc
     5401 ctcaatttaa gacgctgtat ataacagcac ttaataagtc gcataactcc tgagaagcga
     5461 cggctatcct tagaggtagt tgagttattg ccaaaatgtc tcctagcatt ctgaaaggga
     5521 acgccacttt tgtgggaatg ccacctaaac cctctaccat ctcagtgaag catcattgag
     5581 atttcatttt acttccacct cgcggggaac atttttcccg tgttgggcaa ggatgtttct
     5641 ccgctttttt acatttggag aaacaaacta tgacaactca aacttctact caaaaaaatt
     5701 acttcaacct tcatacaaca ggcattggct atctaaacga tattcgtttg gtagagccta
     5761 aaaaaggaaa tcctttttat gcctgccgta tagcagctct ggttggcgaa tctgactctc
     5821 ctgagtacag atattttgat atgaatgtag tcggtaaaga gactgaaaca cttatcaaaa
     5881 gatgccaaaa agcagttagt gaagagaaaa aagtgcttgt gtcatttgta atggctgatt
     5941 tgtggttcga tacatttact tactcaacag atagtaaatt ccacaaaaaa ggcgataccg
     6001 gtgtatctgt aaaaggtcgc ttaatccgca tcaaaatgat taaaattgat ggggaaatag
     6061 cctaccaaga aaagccaaaa tcagatgagc aatctgagta attttaccta agcccgacat
     6121 tttatgttgg gctttattac tcgatgtaca agatttgcgc gtaactttta agaccccaga
     6181 tggcgatgtt actgccgtca atgatctgac tttttcttta aatgccggtg aaaccctcgg
     6241 cattgtcggc gaatctggct caggaaaatc gcaaaccgct tttgctctaa tggggctatt
     6301 agcagataac gggcgaattt caggatcagc aaaatttaat ggacaagaaa ttctcaactt
     6361 gcctgaaaca caacttaacc gcttgcgtgc tgaacaaatt gcgatgatct tccaagatcc
     6421 aatgacctcg ctgaaccctt atatgaaaat cggcgaacag ctaatggaag tactgatgtt
     6481 gcataaaggc atggatcata aaactgcgtt tgaagaatcg gtacggatgt tagatgcggt
     6541 gaaaatgccg gagtcacgta aacgtatgaa tatgtatccg catgaatttt ccggcggtat
     6601 gcgtcagcgt gtgatgattg cgatggcatt actttgccgt ccgaaactcc tcattgcgga
     6661 tgaacccaca actgcgctcg atgtgacggt gcaagctcaa atcatgacct tattaaacga
     6721 attaaaaagc gagtttaata ccgccattat catgattact cacgatctcg gtgtggtcgc
     6781 aggaatttgc gataatgtgt tggtgatgta tgtaggcaga accatggaat acggcaccgc
     6841 tgagcaaatt ttctataccc caagccaccc ttattctatc ggcttaattg gtgcagtgcc
     6901 tcgcgttgac ggacaggaag aagaactcat tactattccg ggtaatccac ctaacttatt
     6961 acgcttaccg agtggctgtc ctttccaacc acgttgcgtt catgcaacta aacagtgcct
     7021 caaagcaccg gcgttaacct tgttaaatga tcaacgttta cgcgcttgtt ttgtccctgc
     7081 cgagcaatta atccgaggta acgcatcatg aacacagaga aaaaagtgat cctagaagtg
     7141 aaggatttaa gcgttcattt caacatcaaa gatacgaaat ctttattctt tgctaaacac
     7201 caaactttaa aggccgtcaa tcatgtctct tttcagttat acgaaggcga aaccttaggt
     7261 gtggtcggtg aatcgggctg tggaaaatca actctcgcac gcgcgattat tggcttagtg
     7321 gaaagctctg gcggtaatat tttatggcta ggtaaggagt taactcaaca aagtgcgaaa
     7381 gaatggaaaa cgatccgtag cgatattcaa atgattttcc aagatccttt agcctccctg
     7441 aatccacgga tgaccattgg cagtatcatt gccgaaccat taaaaattta ccaaccccat
     7501 ttaaccgcgg aagaagtgaa agaaaaagta cgcgcgatga tgatgaaagt gggcttatta
     7561 cctaacttaa tcaatcgcta tccacatgaa ttttcgggtg gacaatgtca gcgtatcggg
     7621 attgcgcgcg ccttgattgt ggaaccaaaa ctcattattt gcgatgagcc agtgtccgcc
     7681 ttagatgttt caatccaagc acaagtagtg aatttattga aatctttaca acgcgaaatg
     7741 gggctatcat tgattttcat tgcacacgac cttgctgtgg tgaaacacat ttccgatcgt
     7801 gttttggtca tgtatcttgg taacgcggtg gaattaggtg actatgaaca agtctatcac
     7861 gatactaagc atccttatac caaagctttg atgtctgcgg taccgattcc tgatccgcaa
     7921 ttagaacgca ataaacaaat tcaattgctt gaaggcgatc tcccttcccc gattaatccg
     7981 ccgtcgggct gtgtgttccg cacccgttgc ccactggcag atcaacactg tattgcagac
     8041 aaaccgctcc tcaaaggacg agataatcac gttgttgctt gttttaaagc cgagtaatac
     8101 acacaaccgc acaaaaaagt gcggttgttt ttttgcgaat attcacagtc agactttcgt
     8161 gatactttcg ctctgagata aggattacga tacagagaag ttattcaaca atcacacgtg
     8221 cattgatcgg ttgcactgga cgattattgt gtcctttcat caaggtcgca aacgcctcaa
     8281 cttgagattg gctaatgttg tcatagtgtt ttaacactaa ccaatttaca ccttcagaac
     8341 aaggtggtgt agttaatgac ccgctgaaac gatagtaatc gcgttttttc ggaattaatg
     8401 ttgctgggtc aaaagcggta ctaagcgtat atttctcccc tgctttttgc gggaagtttt
     8461 gccaaattgg ggctaaccat tggttttcac ttgataatac atagagcacg ccaagcacag
     8521 caagattccc ttcattgtct gcgtgtacaa aatgtgcttc catcgggaag gatttacctc
     8581 aaattaagtt ttcactcggc acatggaaat ggaattgttt aagggtaaaa gtctttccat
     8641 taagctgcat gcgattactt gcctctttga aatccacttg aatagtatgc ccattattga
     8701 caatctccac attggaaggt cgataatcca gcttaatcgg cgctaactta ccatcaatcg
     8761 tatgcttgat atctattggt gtttggtttt taccttcgcc acaaatacgg aatttgggtg
     8821 aaagttcagc ccaatgttct ggcgattcgt gtccggtgta tccccaatgc gtgtgcgttt
     8881 cttctttctt aagggggctt gtacacgctg tcaatgctaa tgcacctaac gtaagcacaa
     8941 aaaggtttga ttttttcata gcctagctcc tgattaaaag agatgaattg atgagacaac
     9001 ctaaactcaa tgatgcctta atccgagacg ctttcatctt aggcaaaaca acactccatt
     9061 tcaagtataa cttgctcaag aatatatctt agaccgaaat caaatccatt atttcaaata
     9121 aggtattagg cgagtatttc tctaaataac gacgtaaaaa atgttgtgtt tcttggctaa
     9181 tgacttgatc tgtttccgga tacaagttgt atatcacagc atgcacactt actcttttgg
     9241 agttgagtac ttgtaaactc aataaggtat gattaatact acctagcttg ccagaggtaa
     9301 ctaaaatcac ggggtattga tgctgacaaa gataatcaag ggtggtttct tcctcattat
     9361 aaggtacaca caagccgccc gccccttcca ataatacata atcatatttt tcacacaaaa
     9421 gtgcggtgca ggtttcaatt tttttacgtt cgatcgtgcg cggttctaat ttcgcggcta
     9481 aatgcggtga gcaaggataa tcaaacacgt aaggacaagt gataccttgc ttatcttctt
     9541 ctgtaagctc aataccttgt atttttcggt gtatgagtaa atcttcggca attcctcggc
     9601 agcctgtttg gatcatcttt tgcgtgatga cacttgcgcc ttgttgcatc agtttcttgg
     9661 cgtaccaacc tgtggcgatg gttttgccaa tatcggtgtc gatgccagtg ataaaaaaaa
     9721 cctttgccat actttttcct tctaagatta agttaaacgc gccagcacat acatcggcgt
     9781 gtaagtgagt tctactttgt gttgtgcgtt acggtaatgt tgttgatatt gttggcaaaa
     9841 ggcgtgcaat tgcccttttg tccacctttg ctgctgtgtg gcagtaacgc ctgtttgttt
     9901 caaatgtttt aacaccgcta agggatcatc aaagtgcaac acaatctcct cgtgttcgag
     9961 ggctaaaatc tcaaattcag gcactagcca cttttgccag tcctcaagac gtggataagt
    10021 taaccccaca ccggtgaggt gtttgatctg tgttaaattc tcgggcgtga aagtactgaa
    10081 taacaaaaat ccgtgctttt ttaagcgcac tttacaacgc gcaataaaac ccgctttatc
    10141 ttcaaaccat tgcaccacgg aagcactggc aatcaaatca tactgctgcg gaaagggaag
    10201 ttgttccgca tcgccacaat ggaagtgaaa aggttggggc aacactgatt gcagatgctc
    10261 tcgcatatcg caaaggtcat tcagctccca gtgttcaacc tgcaactgtg acattaattg
    10321 cttcgttaat ccaccagtac cacatccaat ctctaatgca cgttggaatg cttgtctgtc
    10381 gatatacgac aataaactaa gcaaacgttg gtgaatctgc tgttgtgctc tcgcttgctg
    10441 atcatagttc gatacagcgg cagaaaaacg ctgtttaatg cgtcctttat caatacgatc
    10501 catgtgctac aaccaacaag tttcccaatg ggtgaagtga ggaaacaaca aatgctcccc
    10561 tgcgatttct gttattgcac aacgatcctg ccaataagcc cgctgatttt gtgcagggaa
    10621 aattttatct tttgagccga caatcgcctt gctccacgag attaaatctg tacggtgatc
    10681 ttgttgtaaa aaggtataaa gtgcggtcag ttcagcatga atttcctgta atgtgggacg
    10741 catggaatag tgctgataat ctcttaacag cgtttttgaa ccacaaatac gacgttcaaa
    10801 tttagtccga ttatcttcat ttaaaccaga taacgtgcca gcaaaaatgg cttcgggaat
    10861 accatattgg tcatcacaag gtaagcccgt gccattgatc gccgtagcag atagcagtgg
    10921 ataaccttgc atcacacgct ccgctaccca cacgcccatt gaccatgcca ccacgcgaat
    10981 atgttgataa gcggaaaaat caacatcgca atgcagatct cgataatcat agcaaaggca
    11041 taaatcataa ttttccggca gaatcaggtg ctcgaccacg ctcggcggtg tgccccaccc
    11101 tgcaaaatag acgatcagat tgtcgccttg ctgtgaaata tgttgtgttt tcattcagca
    11161 agctcccgta aacaagcaat aaattgttcc acttccgcca cttgcatatc tgcagtcagt
    11221 gaaaaacgaa tacgagaggt tccttttggg actgtcggcg ggcgaatcgg taagcaataa
    11281 tagccttgtt gttgcaaacg ttgcgccgtt cttactgtca gttcgttatc gcccaaaata
    11341 taaggtacga tacaactttc acttggcatc gggacttgaa aaatgtcaac aatcgcttgg
    11401 cgtaaatgtt gactaagctg agcaagatgt gcgcgttcgg cttgcaaatg tggtaattgt
    11461 tgaaagacaa aatgcgtcca tgccacatta aacggcggaa gtgccgtgga aaaaatcaat
    11521 gggcgcattt tattaaccaa ataatctcgg atcacttgat cgcaaatcac ataagccccc
    11581 atagagccca acgccttgcc aaacgtaccg actaaaatat caatttggtt gatacagcct
    11641 tgttgttctg ctaaccctag ccccttctcc cccaatacgc ctatcgcatg ggcttcatct
    11701 acatataaca tcacttgcgg atagcgctgt tttaatgcga ccaactgggt gaggtctgca
    11761 cagtcaccat ccatactaaa cacggattcc gtgaccacga taatccgctc aaaagtgcga
    11821 tcattttttt gcaaaatttg ttcaaggtgg acgtaatcat gatgacgaaa acgcacaaac
    11881 tcacattgtg ccaagcgaat gccatcaatc atactggcat gtaccagttt atccgccaca
    11941 atcagcgttt ttttgtccgc taaagcaggc aaaatcccaa tattagcatg ataaccgcta
    12001 ttaaatagca aggcagtttc tcgtccaaat gcctgcgcca ttaagctttc tagctcatca
    12061 taaatcggaa aagagcctgt taataaacgg gaggaagaag aagtcaatgc agggagttga
    12121 tcttggtatt gcgtgaaaaa tgcctgacgc aaggcttcgt tattggctaa acctaaataa
    12181 tcattggagg acatattcag catcgtattg tcctcacgtt gaataaaacg ccctcggtga
    12241 attagttggg gcagttggcg aaattgattc tgcgccttta aatcagctaa ctgctgctga
    12301 taatacctga tcatagagat tccttatgtg catattcttc ttgaagggtg cgaattgtgc
    12361 catgaattaa ttgagacaat tgatccgatg agatgatata cggtggcatc aaatacacta
    12421 atttgccaaa tggacgcacc caaacgccat tttgcacaaa gcgtggtggt aaacttgcca
    12481 tattgacagg cgccttcatt tcaatcaccc caatcgctcc aagtactctc atttcagcga
    12541 cacaatctaa ggcttccgct tggcgcaatt cttgacgtaa ttgctgttca atctgttgaa
    12601 tacgttgttg ccaagggctt gccaataaca atttgatact ttctgctgct accgcacacg
    12661 ccagtggatt tgccataaac gtcggaccgt gcataaaaca ttttgcctcg ccagaacaaa
    12721 tcacgtctgc taccgcttgg ttggtaatgg tagcggatag agttaaatac ccccccgtta
    12781 aagctttacc gatacacatc acatccggca cgacattggc atgttcacaa gcaaataatt
    12841 tgcctgtacg cccaaatcct gtggcaattt catcaaaaat cagcaaaatg ccgtacggtt
    12901 cgcataaggc ttttgcttgc accaaatact caggggaata aaaatacata ccacccgccc
    12961 cttgcactac cggttctaaa attaaacccg caatctcatc accatgttgt gccaataaat
    13021 cctgtaacgg ttgcatcgct tcgggttgcc aagcttgccc aaattttacc gggggttgcg
    13081 gtaaaaaata ttgcacgggt agccctttgg caaacaagct gtgcatgccg gtcacgggat
    13141 cacacacgga catcgcatgc caagtatcac catgatagcc cgaacgaatc gtagcaaatt
    13201 tatagcgctg tgtttcgcct ttagcatgtt gatattgcac tgccattttc atcgccactt
    13261 ccacggcgac cgaaccacta tcggcaaaga aaattttatc caaactttcc ggcaagatgt
    13321 tgaccagttg ctgcgccagt tctaccgctg gttggtgcgt taatccacca aacatcacat
    13381 gactcatttg actcagttgt tgcgtggcag cgtggtttaa acgggggtga ttatagccat
    13441 gaatcgctga ccaccacgat gacatgccat caatcaactg ctgaccgttt tttaactgga
    13501 tatacacacc ttcggcacgt tctactgcaa ataagggaaa atccgcattt aacgccgcat
    13561 aagggtgcca aatatgttgt cgatcacatt ctaaaagttg ctgctcattc atcgtttcac
    13621 ctcggttgtg aatcgccctt tcaactggcg taaattcacg caaatactta cataaacgga
    13681 ataacttgtt ataatcgcca agcatttggc ttttcaagcc cattattcta acttaaaaag
    13741 atgatttatt actatgaata tattagaaaa aaaagcacaa ctgagttact ggcaacggat
    13801 taaaattgcc tttcaatatg tgatgccaca actttattta acacgtcttg caggctggtt
    13861 tgcaaaacag caatggggtg ctgtcacaca ttttgtgatt aaattattcg cgaaaaaata
    13921 ccatgtggat atgagtgaag ctgcaaaacc aaatttcagc gactatgcga gctttaatga
    13981 attttttatc cgtccattag cggacaatgc ccgtccaatt aatcaaaatc ctaccgcact
    14041 ttgtttaccg gcagatggtc gtattagtca gttaggacac attgagcagg atttattgct
    14101 acaagccaaa ggacattatt tcagcttgaa cgatttgtta gctggcgacg aggcgctcgc
    14161 tcatcatttt aaagatggcg aatttgccac cacctacctc tcaccacgcg attaccaccg
    14221 tgtgcatatg ccttgtgatg ccaccttatg caaaatgatt tatgtgccag gtgacttatt
    14281 ctcagtcaat ccatttttag ccgaacacgt gccaaactta tttgcacgta atgaacgcgt
    14341 gatctgcgta ttcgataccg aatttggtaa aatggtccaa attttagtcg gcgcaaccat
    14401 taccgcgagt atgagcaccg tgtgggcagg tgtgatcaac ccgcctcgtc cagaaaaaat
    14461 caccacctgg acctatgaag gagaaagtgc ggtcaaatta ctgaaaggac aagaaatggg
    14521 agcctttcaa ttgggctcga cggtgatcaa tttatttgaa aaagatcgtg tgcaactggc
    14581 atctcattta caagtggata gcccggtacg tatgggtgaa attcttgccc accaaaaata
    14641 atttttaacc acaataggaa aaacaatgag tcaacaattt ttcgaccaag tgtcattaga
    14701 aagccaactt gaagtgagcg ccgaagccgt cgcgaaaggg atctttgatg tgttaaatca
    14761 aaatccacca gcacttgagt taaatgaagt gagtcatgcc ctacagactt tgcaacaaca
    14821 agcgaaattc aaagaaattg aagccgttgg tgcacaattc ttagctgaaa acgcgcaaaa
    14881 agcgggggta aatactacgg aatctggttt acagtatgaa gtgttagtgg ctggtgaagg
    14941 gcaaattccg gcacgcgaag acaaagtgcg tgtgcattac actgggacat taatcgacgg
    15001 taccgtcttt gacagctcag taaaacgtgg tcaacctgca gaattcccag taaatggcgt
    15061 gatcgctggt tggattgaag ccttgtccat gatgccagtg ggttcaaaat ggcgtttaac
    15121 cattcctcac aacctcgctt atggcgaacg tggtgctggt gcctccattc cgccattcag
    15181 taccttagtg tttgaagtcg aattattagc cattctttaa agctaaacgc tagacataaa
    15241 aaagtgcggt cattttccgc acttttttat gtctagcgtt tatcgtttca agccttgctg
    15301 atagccgctg ttccacgaat ggcggaactt gtccggcaca gccttgcccg tttgttcttc
    15361 acagtaagcc gttcgttgaa tcaacatttt ttgttggtgt tctcgaaatg attttcccac
    15421 acgatagccg tccaaatacc aatcatcatt atcttgacat gccatggtat taatccgttt
    15481 tgtattcccc gtcactgcaa aagatttgcc tgcctcaata cgttgcaccg catgttcaga
    15541 gtatgaagca cagctcacca gcgaacctag tacactgaat atcaatactg ctttccaatt
    15601 catcttgtta cctttctttt tctgatttat aacacaatca tatcgtctcg gtgtaccgca
    15661 accgccccat attcataacc gagaacctgt tcgatatctt gcgaatgttt gccttgaatt
    15721 aaatggagcg catcgctgtt ataacgtggc atccctaagg caacatcctt gccttgctga
    15781 gtacgaattt ttaccacttc accgcgtgaa aagcgccctt ccacgtttac aatcccagca
    15841 ggcaataacg atttatgttg tattaacatc gcgttttccg cccccgcatc aatggtaata
    15901 ataccggctg acggcgccgc atatagccat tgtttacgac tttctaagcg atctgcttgc
    15961 acactaaatt tggtgccaat ggcttgccca taagctaagt ccacaatcac attcgggcga
    16021 ttacccggtg caataatggt ttcaatgccg gagcgggttg ccacatccgc ggcactgatt
    16081 ttggtggaca tgccacctgt gcctaacgag gtgccacttc cgcccgcaat cgcgcgaata
    16141 tgatcggtaa ttttgtccac gtcggcgatc aatttggcgt ctggatgttt gcgcggatca
    16201 cgatcaaata aaccttgctg atcagtaagc aaatacaact gttctgcttg caccaaaatt
    16261 gccactaacg cggataaatt atcgttatcg cctactttaa tttctgacgt agcgacagcg
    16321 tcattttcat taatcactgg aataatctga ttgtctaata aggcgcgcaa agtatctctg
    16381 gcatttaaaa aacgttcacg atcttcaatg tcggcacggg tcaataaaat ctgcccaata
    16441 tgaatatcat aaatggcaaa caatttttcc cacgtttgaa tgagctgact ttgtcctacc
    16501 gcagccaata attgtttcga ggcaatcgtc ggtggcaatt gcgggtgatt taaataatga
    16561 cgacctgccg caatcgcccc tgaggtaaca atcactaaac gaaagcccgc ttgatgaagc
    16621 tgtgcgagct gacgcacaat atctatcata tgaggtaaat ttaacttcgc cgatccttgc
    16681 gtaagcgtac tggttccaaa ttttacaacg agtgttttgt cgattttcat cttctctatc
    16741 ttcttatttt tgtcgcacaa aattgaagct aacttaccat taatcatcag aaaaatcatc
    16801 tcgtttactt gctattttct atgatttagc acaaattttt tctcactaaa aggcatataa
    16861 taaagtttct tgatttttca gctaggaacc cgattatgac ctcaacctct tcacaagaaa
    16921 cggagcggca acgagaaatc actcgccttt gtgttcaagt tgcccaactc ttatacaaca
    16981 tggtgcagaa agtgccatcg ttgtgcaaat ggcacatcgc ttaggcttag ccctcggtgt
    17041 agatagcgta gaatgtgccc tcacgccaaa tgccattatt cttactacat tagcccaaga
    17101 ccattgcatc acgacagtcc gtaaaaacca agacaaagga attaatatgc aagtggttac
    17161 cgaagtgcaa cgtattgtga ttacagcaga acgtaaaatc tatgatatgc aacaagtgca
    17221 tgccaaactg gctcgcatta aaccgcttaa atacaatcgc tattttgtga tgttgatgat
    17281 tggcgtatcc tgtgccagtt ttgctcattt atctggtggg gacgcactag tttcattggt
    17341 tacatttctc gccgcaacac tcgccatgta tgttcgccaa tcacttgccg caagacacta
    17401 taacccctta attgtatttg cggtgaccgc ctttgtcgcc tcactaattg cgggtatcgc
    17461 gttgaaatat aacatcggca atcagcccca aattgccttg gcatccagcg tattattgct
    17521 cgttcccggc tttccactga ttaatgcact cgccgatatt ttaaaagggc atatcaatat
    17581 ggggattgcc cgttgggcac tcgcgacaat cctcacattt ggggcatgct taggcattgt
    17641 atttgcgctg agtttattct tagaggaaat ctgtatgcta aataaattca ttcaagatga
    17701 atttctacgg ctagatcaac gttatgcagc ttggtatcaa agtgaagaaa cggatgaagt
    17761 tgccagaatg gggattcaag aaagtgagcg gattttgaaa attctgtatg cactgaaata
    17821 taacgaagac cctatgcaaa atggtttagg ctatattgca tttatggcat caaaaggcca
    17881 ataatttact tatctcaaca atttacccac cggggaaact cctcgatggc gtttccccta
    17941 ctttatagga gaaacgctat gactattaac acttatgcaa aatttgcacc taccgttttt
    18001 gttgcaaaat gctctgaatc acataccaaa ggtgaaatta ttacgctaac cagcaaatat
    18061 ggtaatgaaa ccgaggttca agtacataat cttgttaaac aagatggcga gcattatttt
    18121 tattcattta ctcgttgtga tgggatggat agtcagtcat ttgctcaaca aaaagcagaa
    18181 cgctatcaag gctatgcaga taatgcagcc aaacgtagtc aagattgggt aaatgccgct
    18241 aatgaggggt gtgatttttt gagtttaggt gagccaataa aaattggtca tcacagcgaa
    18301 aagagacacc gtaaccttat tgaacgtaat gctcgccgta tggacaatgc ggttgcagaa
    18361 atgaacaaag ctgcaagtta tgaatctcgt atcgcatatt gggaaaaaat ggcaggaaaa
    18421 attgacctgt ctatgccgga aagtctggaa tactttaagt ttgaattgga taaagccaaa
    18481 acaaagcaca aagaattgaa ggacaacccc gagaaacgag agcatggtta ttcactgact
    18541 tatgctaaaa aagcagttaa tgaacttgag aaaaaagtaa aattagctga attactttgg
    18601 gcataattat ttacaaagga gatgacgata tgaatcgaca aggtatttat attaatggca
    18661 aacaggtgtt tgattcacaa ggcgaagata ttcaaagcat tggatttttt ggctgcaata
    18721 ttcaaacggt cgtaggcata aacaaaggaa gtattttagt ctcgtccaat agtaatcaat
    18781 tattggtgaa tggacagtcg attgaattag gttctgagcc agtcctcaaa attgaggttt
    18841 tgggcaatgt gcagaaactg agtgccggtg ccgcagatgt ctctattaca ggagatgccg
    18901 gcagtgttga cacagttagc ggtgatgtct ctattaaggg aaatgttgcc tcttatgtga
    18961 aaaccgtgag tggtgatgtt cgctgtcgtg ctattaccgg ttcggtggaa accgtctccg
    19021 gtgatattta tcgctgatat ttactcatct tattatttac caccctattg ggaagcaatt
    19081 ttcccaaggt gggcagattg tttcccattt ttattttgaa tttggagaaa catgatgacc
    19141 actattaacc tttcacaaca agaacgtgag ttagttgaat ttgtctggaa taaccgtaat
    19201 acaccttggt tttttcactg tagtgtatcg aatgtgtcga gaaataatac ctctcggcaa
    19261 attgctgtat gtgtcattaa ggataatcaa ttgattagcc ttgatgaatt gatagcaaaa
    19321 ttatgtggtt ataaactcag acaaaacgga ttatacgtta ctgttcataa tatggatgct
    19381 gtttttgata ccttatatcg ctttatgtgc aagctaaatg atgagatgga ggcaaatgaa
    19441 aatgtttgcc cttatttatc attagctcac tatgaagtgt tttgattggt atcagaacta
    19501 tccaccctgt cgggaaacaa tttttccgat agggcagatt gtttcccact tttattttga
    19561 atttggagaa acataatgat agacaccttt tttgaccttg acgacacctc atgctattta
    19621 ggtaactggg aggatatact gaaagaatat gatgattgtc tttcagttga agatttttgg
    19681 cattccgcta aagaatttga taccgtacct cattttggca acctatacca ggagcgtgtc
    19741 atccttaagt tgattgctcg attctgtatt gagctgaata ttgagcaaga ttcaggttta
    19801 gtcgaatttg attattatat caatgcaata gatacgcatt tctatattaa tagccaaaga
    19861 atctgtgata tagatgattg gaatgaaatg ttagataaga ttcgtgaaga aatgacgccg
    19921 gctaaattag ttgcctaatt tttgcaaatt tttactaaaa atgacgctta ctgtcgtctt
    19981 aattcaccca cttggggaaa tatctcccca atgggatgac atttcccctc tttctgttta
    20041 ggagaaatgt atgacttttg agcttggtag agtatgtgca actcgtgcag ttgctgaatg
    20101 tttcaatcaa catacattag ttgatctact tcatcgctac tgcagtggtg attggggaaa
    20161 tttgtgtgat aacgacaaga aagccaatgt gaaagctatt gaacgcggag gacgcatttt
    20221 agctcgctat gatgtggaag atgaaacact ttatgtaatc acagaggctg atcgtagtta
    20281 cactacaatt ttatttcctt atgaatatta atggagcttc catttatacc ctacattttt
    20341 atgttatcaa acgccgtcaa atgatggcgt ttttctattt tatatctttc gattttatag
    20401 ttgtttcaat atgcaatttt aattattttt cct
//
LOCUS       DANLXF010000011        20039 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.011, whole genome shotgun sequence.
ACCESSION   DANLXF010000011 DANLXF010000000
VERSION     DANLXF010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 20039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20039
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.011"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..588
                     /locus_tag="PXD66_000247"
     CDS             <1..588
                     /locus_tag="PXD66_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL6318584.1"
                     /translation="GDYLESSDAQIFRDKYQELEFKPNPDFNGPYYGLDFGFANDPTA
                     CVKCWVFDDDLYIEREAGKVRLELDETAAYLANALPDLSNYTVRADSARPESISYLKR
                     HGLPYIQPVPKWKGSVEDGVEHIKSYRKVYIHPSCTEVLKEFRLYSYKTDRLSGDVLP
                     IIIDAHNHYIDAIRYALNPLIQARGEAKQCALKVY"
     gene            601..2004
                     /locus_tag="PXD66_000248"
     CDS             601..2004
                     /locus_tag="PXD66_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4055 domain-containing protein"
                     /protein_id="HDL6318585.1"
                     /translation="MPNSISADFAALKAKTKIIDDLLGGTQKMRQAGQTYLYKMKMEE
                     EDGYKNRLNRSTLYPALRETLSQMLGRVFFNPINVEDVHKQIEPLFDDIDLEGNNLDV
                     FASRWFYAALAYGVSYCLVDFVRVENARSLAEEKAQNARPYLVHIKPQQVLGFKTAKI
                     NGKTAFTQFRYKETVTEDDGEFASKQVNYIYVYEIGRVRKYKNDEGGLSLVADMQIKA
                     QNTPLAFVPIVPFITKPIDVWGMGEPPLMELAYLNIKHWQSQSDQDNILNIARVPLLA
                     IFSDTQIKSLEIGSSAISLPKDSTMQFIEHSGSSINAGLTSLKELEDQMKTAGAKLLT
                     KTALAMTDSQAKDEAGKEISQLRHYANKFEDALDLAFEYIGHWLGIGVDEVGTSQISG
                     NIDNDLDPNASMATIIQLRNAGVISNHSTFEEAKRRGLLSDSAKWEDEQARLQAEGLS
                     DDFTNPFNSNQSENAAS"
     gene            1952..3613
                     /locus_tag="PXD66_000249"
     CDS             1952..3613
                     /locus_tag="PXD66_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HDL6318586.1"
                     /translation="MTLQIHSIRTKVKTPLAKRIAHALTDRKILQFRFDAHLRNQVWQ
                     RLTKTQKALLNRINAAGIDALPKRELDKLLKELKAEIAKNYQETTAYIQPELSGFFAV
                     EKQALTELYNDEIGYDFFNQVPSYQRKATQQATIIAGMPLESWFEKQGSELSFKFEST
                     IRQGLLEGRQTPEIAKATAELFGISRRHADTLVITAVAKVADDAHKALRDENLDLIKG
                     EMHLSTLDTRTSTVCQVRDGKMWDTDYKPIGHSLPYQRPPLHPRCRSILQLVMKDWKE
                     LGFDDVKEMPKSTRASADGQVSEKTNYEEWLSSKTPEEQDKALGKGKADLWRRGVITF
                     SDMLDQSARPLTLKELYSLKNSSSVDKMDSVELVRKKAVAVEPMITRDVVSIITASGG
                     EPAGLDFRLKSLSSLQRKIDTEIMAGVSKEQAIASIRDVIRYTAILDEQRFVEQYQKM
                     QKDLEKQGYSTIIVKNTWKSNNAYKGINTFVSTFIEKNNIIFELQYHTKQSFELKNGK
                     LHELYEKFRDLNIPLAKKSEILLEMQKLSANLKEPKNIDLIKEKK"
     gene            3613..3831
                     /locus_tag="PXD66_000250"
     CDS             3613..3831
                     /locus_tag="PXD66_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318587.1"
                     /translation="MYQYYLAQVGNEQQKLIRGMPDNLLSFSVYEPEQEDWDNKFGPF
                     WADKILISGFDAYEEISEKEAIRFIKVH"
     gene            3832..4245
                     /locus_tag="PXD66_000251"
     CDS             3832..4245
                     /locus_tag="PXD66_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL6318588.1"
                     /translation="MCQAEQFARQLHYGQTDKAGKPYIEHLAFVAENISTKNEETEAV
                     AWLHDSVEDTQCSINDIRHIFGDVIADAVEAITKKQGESYFCYLDRVKSNPIAKVVKL
                     ADLTHNLCLERLPKITEKDLKRAEKYKSALEYLQA"
     gene            4363..5097
                     /locus_tag="PXD66_000252"
     CDS             4363..5097
                     /locus_tag="PXD66_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318589.1"
                     /translation="MKLKLDENGHVVVENGMPVYIHDDGKEIPFDALKATQKITQLNA
                     EAKQHREAKEKAEADLKAFSGIDDPKAALEALKTVANLDAKKLIDAGDAEKVKAEIIK
                     GYDEKLAVATAQAEKLQQQLHAELIGGSFARSKYAQEHLNIPSDVVQAFFGKHFSISD
                     DGKVVAKFADGGEIFSRTRPGEKADFEEALEALVSAYPNKDAILKPSGSSGAGAGGGS
                     RSSSMPKTLSDCKTDEERIAYLQANS"
     gene            5163..6110
                     /locus_tag="PXD66_000253"
     CDS             5163..6110
                     /locus_tag="PXD66_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318590.1"
                     /translation="MAFDLQVFNKQTQLALTETVDQAIEKFNAASGGAIILQNKPAEG
                     DFDIRASFKGIAGLVRRRNVYGTGTVDAKRLEQLLDVAVKVAAGTAPIEYQPSQYKWI
                     LLNPELAAIEIGTQLAKARVADMLNTAIAGTVAAISGNTAMTLDEKTAAPTFRILNKG
                     AAKMGDRAGSLKAWVLHSTTLHGLYDNALTNAENLFKYENVNVIRDPFGRVFVVTDSP
                     ALMASDGTFNTLGLVENAVVVSGNNDFDSVLVPKTGGENLSAVYQAEWTYNLGILGYK
                     WDMTAGGKSPNDTALGTPTNWDKSVTSNKDTAGVLVKTK"
     gene            6170..6520
                     /locus_tag="PXD66_000254"
     CDS             6170..6520
                     /locus_tag="PXD66_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318591.1"
                     /translation="MKRILYFTTDFSAKNIAFAKAHGVLMRNLNAYHPSDTLENADAV
                     CGNVPQAYQHLPLFELPESNQKSGQDLKTLKTEEIKEKLTELGIAFEANAKKETLLTL
                     LTQAVGRDGTNDAK"
     gene            6510..6947
                     /locus_tag="PXD66_000255"
     CDS             6510..6947
                     /locus_tag="PXD66_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318592.1"
                     /translation="MLNSYMTVEQANAYHDIRMSREAWNALSDQEKQQRLVSASDFLD
                     FNYRFSGEKADLTQEREFPRKGFEAIGIPKAVQFAVCELALQDNLTANQEAQMSSVRV
                     GPVSVNYDNSSPQNSVNRFEYVRQLLSTWLDRSSFGTVKLERS"
     gene            6947..7318
                     /locus_tag="PXD66_000256"
     CDS             6947..7318
                     /locus_tag="PXD66_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318593.1"
                     /translation="MADFYGNLGKISHSLIKQFGQPCSVTINEQGRYNPATGERKKGS
                     STVHAGYCLFDNLAFDFRNQQSVSVQQGDVMIYLTADCKPVLNAVIEANGEKWSVINV
                     QPIRPAATTMIYQVQGRKADG"
     gene            7311..7724
                     /locus_tag="PXD66_000257"
     CDS             7311..7724
                     /locus_tag="PXD66_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDL6318594.1"
                     /translation="MGSFHAQIARFVEQTQQKADRALRVIALQTLSGVQKKSPVDTGQ
                     LRRSWTVALNGFPSSYNGSQTVLNSAKFGDMIVIATNKPYAPMLEYGLYPNPPKKPTG
                     KTKNGYSIQAPNGMVRITVQEMQAFIRNNPQLGVS"
     gene            7724..8116
                     /locus_tag="PXD66_000258"
     CDS             7724..8116
                     /locus_tag="PXD66_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318595.1"
                     /translation="MRPIIRSVLEQHLAELNPFNTVWEGIIDSPKLPYQTVSMTTSTN
                     ETATIGNTPKAKQSGFLQVTLYYPLDQGTKAIEERADEIFRHFFGQVWIEENVQILVQ
                     QPPLVASLYRLDNSIALPVTIYFTAYEL"
     gene            8126..9148
                     /locus_tag="PXD66_000259"
     CDS             8126..9148
                     /locus_tag="PXD66_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318596.1"
                     /translation="MSILGNSQGTKRTVVFAKESSFGTKAAPTGAKIMPRVETSLNTN
                     FESYQSEEIRDDLQRSASVIGFEKVEGDIKGELAAGQWAAFFAAALRGSFTAQAKKPI
                     ITKTTDGTGEKNGKILIVPKTSHTSDSFTLEDVFKDINVSRQYLGCRVSKLSLDVQPN
                     GIASITVSFLGQRGEESTTAYFTNPTDLTQSGKLAGVKGSLQVNKRPVGLVTAFKLDI
                     DLGASSEPVLGAKYAPDVFIGTVAVSGSFTMYFQDKTMIDAVRNGTDLSLALRMDAEE
                     RTNTDYLTIILPGVKVTSNELDDGAKNIMQTLNFDAFPAVWDSASQIDNVLKVATTMI
                     VQDSLA"
     gene            9238..9639
                     /locus_tag="PXD66_000260"
     CDS             9238..9639
                     /locus_tag="PXD66_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318597.1"
                     /translation="MNLNALSKEKQLAEHRFELVHPVSGEPLGVFMSVVSAKSDKAQR
                     FAEKQLRQEQMRELENAKSRKPKFKGLGDMIAESRAMAIDRLTGWENVEWNEKPLEFS
                     DQNAELLLTECDWIVEQIFEHSNDLGKFLKS"
     gene            9654..9980
                     /locus_tag="PXD66_000261"
     CDS             9654..9980
                     /locus_tag="PXD66_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318598.1"
                     /translation="MYAEKTFKLDKKLKGSESTLREHLQAIEMQTGITPEELHNPEPS
                     PAVAYLLGWFFELAMSRQSGMSLNPISYTEIDAWSRLFARHLEQWEISVIKQLDMIYL
                     NVQQTE"
     gene            9982..10305
                     /locus_tag="PXD66_000262"
     CDS             9982..10305
                     /locus_tag="PXD66_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005714385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6318599.1"
                     /translation="MDTFNFQPNWGLNVQRKPEVMKIAFGDGYEQVAPKGLNHNLRTY
                     SAVFTGTEARIKQIEAFFERQGGYKAFLWTPYGGKEGKFRCEEWEVSISSGKWTLSAT
                     IREVL"
     gene            10392..11084
                     /locus_tag="PXD66_000263"
     CDS             10392..11084
                     /locus_tag="PXD66_000263"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318600.1"
                     /translation="MKKIFVSALLLTCSLSALGNSSYELIIDSMQDLEKHKPEILSIP
                     EADRNTFIRYMMRKELRIKLNQGRLADGLTVREAIQNQQAFEKDRNSKMQQEKQKKET
                     EKAEKEKAVTDFNQKFELSITGARKAKNALNQDILLVSLSLKNNSDKPIIAVQPDIQF
                     VLGEDKQTISSMRPKKFENGIPPNGSEIFEFGFDLDDMFALKAEKNIEKLRAVFQTAE
                     VLFADGTTEKLQ"
     gene            11148..11972
                     /locus_tag="PXD66_000264"
                     /pseudo
     CDS             11148..11972
                     /locus_tag="PXD66_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691083.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
     gene            14016..14174
                     /locus_tag="PXD66_000265"
                     /pseudo
     CDS             14016..14174
                     /locus_tag="PXD66_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251214.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
     gene            14182..14898
                     /locus_tag="PXD66_000266"
     CDS             14182..14898
                     /locus_tag="PXD66_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDL6318601.1"
                     /translation="MPISISNQMKLDLAKLEQNAMLDLYEVDLRNLKDKSGNAGRVYR
                     FYSGLNELKTSIVWQGRTYDPYPIEASGFERNSSGPSNRPTLTLSNLFGLITSIANQF
                     DECIGAIVRRHQVYAQYLDAVNFAGGNAKADPNQEIISHFVIEQLTSLTRETATFTLA
                     LPIETDNAKIPSRIIMADTCTWIYRSAECGYTGKQYFDEKDKSTSDPKADKCSHCLNG
                     CELRGNQRNFGGFVSVNKLG"
     gene            14902..15633
                     /locus_tag="PXD66_000267"
     CDS             14902..15633
                     /locus_tag="PXD66_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL6318602.1"
                     /translation="MELEQQIIDYALQHEPHEMCGFVVFDGNKNRFIPCENQAEDKAN
                     YFEISDLDYIKAEEKGELVAVVHSHPEPNGKLILSVLDRKMQVQTGLDWWLVHNRQIH
                     KFRNVPHLIGREFKHGTMDCYTLYRDAYMLAGYEMDEFERQDDWWHSGQNLYLDNIQG
                     QGFERVETPQIGDVILMQVGADVPNHAAIYIGEQMVIHHSPNRLSKRDLYDGYWLRHT
                     HSIWRHKLAEKLDFDGILNDIAVQD"
     gene            16267..16929
                     /locus_tag="PXD66_000268"
     CDS             16267..16929
                     /locus_tag="PXD66_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005826391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HDL6318603.1"
                     /translation="MTTQTKLSTFNFETQQIRVIAVNGEPWFVAKDLCDTLGISNPSK
                     AILNLDDDEKMISSDSNLKLGSAGNGAQSLSLVSESGMYTLILRCREAVKKGSVPHRF
                     RKWVTAEVLPQIRKTGAYTKPVEQEPKFTHTLTSDELRDLAWLWFEASKMAETMNTLY
                     QPLMAIGSNLAPMVYSQGVEYGNHIQRMGKLIYRLTYGVDFSNNPTPFRHLQQGVALP
                     HR"
     gene            17012..17602
                     /locus_tag="PXD66_000269"
     CDS             17012..17602
                     /locus_tag="PXD66_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDL6318604.1"
                     /translation="MVKVKFYGHLRKFGTDFKLEVKDTAEAIRALCTQIRGLREALRD
                     GVYKVRIGKQYLDPSALEKGLFYCLKKGQTIHFTPVIKGAKSGGVFQAVLGVALIGAA
                     FFLGPVGWGVLGASSAMMMGAMGASMLLGGVSQMLTKMPKAPTMGNETEKESSTAFSN
                     LNNLVAQGKPVPLAYGLIRTGSLVISQGVETMTVEV"
     gene            17605..>20039
                     /locus_tag="PXD66_000270"
     CDS             17605..>20039
                     /locus_tag="PXD66_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HDL6318605.1"
                     /translation="MGLFSRKRKNHTPKEAPETGRSKQIVNIVELLCEGEIEGLVDGF
                     KSIYLDGTKIQNDDDSYNFNNVSGQLNVGTQDQEVLEGYDSSQNEVSVGVEVKKKNGA
                     IVRTVTDERISRLRLTLGVRSLFHQNNQGDTNTTNVDLKITIGTRQYSHSFNGKYSSQ
                     YLESVVFDNLPPVPFNISVERLTEDSNSQRLQNGTIWSSYTEIIDTEFTYPNSAVAGI
                     SFDSEYFNNIPTRNYLIKAKKVKVPSNYDPVKRTYTGFWDGTFKIAWTNNPAWEIYDL
                     APILSKMLGVEISFDKWALYDVARYCDQLVPDGMGGMEPRFTCNVWLTEVKTAYDLLN
                     DFCSVFRAIPIWTGTEVSVIIDRPRDPVWTYTNANVVGGFERSYSARKSRHNAVQVTY
                     SDKTNGYESAIEYVSDDEEIKKHGLNLSQVTAFGCTSRGQAYRTGKWILETEKREKET
                     ITFTVGREGLMHLPGDIIRVADSHYAGTEIGGRVLAINGRKVTLDREISIDNASYFTY
                     INGEATHSSIKIQSVNGKEITLDSTPTGLETYGVWSLSTKQISSGLYRSISIVENADG
                     TNTITALQHEPQKEAIVDNAAHFVETARTLYKAPQINAVEVSTGYDGKLYISSDISSG
                     DGKLTYDIKITKDGNFYQFKKGLADPNIELSDLPNGDYSVIIYGKNAKGQIVTEKTQT
                     FTIDRPPAPTGVVVTGGLGQITLEWDWVNEVTQTEIFAAETDNFALAKKIAKVTARTY
                     AHTLKGNKVVRYYWLRHARGINVGPFYQQQGVRGESAVDLDARLTELNTQLSRNIVNE
                     VFDVAAPARGLELV"
BASE COUNT         6307 a         3904 c         4645 g         5183 t
ORIGIN      
        1 ggggactact tagaatcctc tgatgctcaa atctttagag ataaatacca agaattagaa
       61 tttaagccta accctgattt taacggtcct tattacgggc tggactttgg ttttgccaat
      121 gacccaacag cctgtgtgaa atgttgggtg ttcgatgatg acttatatat cgagcgtgaa
      181 gcagggaaag taagactaga gctcgatgag accgctgctt atttagcaaa tgctttgcct
      241 gatttgagta attatactgt tagagcagat tctgctcgcc cagaatccat cagttattta
      301 aaacgacatg gattgcctta tattcaacct gttccaaagt ggaaaggctc agtcgaagat
      361 ggggttgagc atataaaatc ttatcgtaaa gtatatattc atccaagttg tacagaggta
      421 ttgaaagaat ttcgccttta tagttacaaa acggacagat taagtggtga tgtactgcct
      481 atcattattg acgctcataa ccactatatt gatgccattc gttatgcctt aaatcctctt
      541 attcaggcaa gaggcgaggc gaagcaatgt gcattaaagg tttattaaaa tgaggtaaca
      601 atgcctaact ccatctcagc cgattttgcg gcattaaaag caaaaacaaa aatcatcgat
      661 gatttactcg gtggcacaca aaaaatgcga caagcgggtc aaacctacct ctataaaatg
      721 aaaatggaag aggaagatgg atataaaaac cgattaaatc gttctacgct ttatccggca
      781 ttgagggaaa cgctttcgca gatgttgggg agagtgtttt ttaatccgat caacgtagag
      841 gatgtgcata aacaaatcga accattgttt gatgatattg atttagaggg taacaatctt
      901 gatgtattcg cttctcgttg gttttatgct gcattggctt atggcgtatc ttattgcttg
      961 gtggattttg tgagggtaga gaatgcccgc agtcttgcag aagaaaaagc tcaaaatgca
     1021 cgtccgtatt tggtgcatat taagccgcaa caagtgctgg gctttaaaac agccaaaatc
     1081 aacggtaaaa ctgcttttac ccaatttcgt tataaagaga cagttacaga agatgatggc
     1141 gagtttgctt ctaaacaggt gaactacatt tatgtttatg aaatcggacg tgtccgaaaa
     1201 tataagaacg atgagggtgg cttatcactg gttgctgata tgcaaatcaa agctcaaaat
     1261 acaccgcttg catttgtgcc tattgttcca tttatcacta aaccgattga tgtttgggga
     1321 atgggtgagc cgcctttaat ggagctggct tatttaaata ttaagcactg gcaaagtcag
     1381 tcagatcaag acaatatttt gaatattgct cgtgtaccgc tattagctat tttctctgat
     1441 actcaaataa aatcccttga aatcggctca agtgcaatat ctctgccgaa agacagtaca
     1501 atgcagttta ttgagcattc aggttcgtct attaatgccg gtttaaccag tctgaaagaa
     1561 ctagaagacc aaatgaaaac tgccggtgcg aaactgctaa ctaaaacagc attagcaatg
     1621 acggatagtc aggctaaaga tgaagcaggt aaagagatct cacagcttag acattatgct
     1681 aacaaatttg aagatgctct cgatttggct tttgagtaca ttgggcattg gctaggtatc
     1741 ggcgttgatg aagttgggac ttcgcaaatt tcaggcaata tcgacaatga tttagatcca
     1801 aatgcgtcaa tggcaaccat tattcagcta cgcaatgccg gtgtcatttc aaatcactct
     1861 acgtttgaag aagctaaaag acgaggttta ttgtcagata gtgcaaaatg ggaagacgaa
     1921 caagcaaggt tacaagcgga aggtttaagc gatgacttta caaatccatt caattcgaac
     1981 caaagtgaaa acgccgctag ctaaacggat cgctcacgca ttaacagatc gtaaaatatt
     2041 gcagttccgt tttgatgctc atttacgaaa tcaagtttgg caacgactca caaaaacgca
     2101 aaaggcatta ttaaaccgaa ttaatgcagc cgggatagat gccttaccga aaagagagtt
     2161 ggataaactg ctcaaagaac tcaaagcaga aattgcaaaa aattaccaag aaacgaccgc
     2221 ttacattcag cctgaattaa gcggtttttt tgcggttgaa aaacaggcac tgactgagct
     2281 ttacaatgat gaaatcggct atgatttttt caatcaagtg ccgtcatatc aacgtaaagc
     2341 tacgcaacaa gcgactatta ttgccggaat gccgcttgaa agttggtttg aaaagcaagg
     2401 atcagagctt tcgtttaaat ttgaaagcac cattcggcaa ggcttgttag agggtaggca
     2461 aacgcctgag attgccaaag ctacggctga attatttggt atatccagac ggcacgctga
     2521 tacacttgtc attactgctg tggcaaaagt ggcggacgat gctcacaagg cattgcgaga
     2581 tgaaaatctt gatttaatta agggtgagat gcacttatca acgctcgata cccgcaccag
     2641 tactgtttgt caagtgcgag acggtaagat gtgggacact gattacaagc ctatcggaca
     2701 cagtttgccg tatcaacgcc caccacttca ccctcgctgt cgttcgattc tgcaactggt
     2761 gatgaaagac tggaaagagt taggctttga tgatgtgaaa gaaatgccga aaagcactag
     2821 ggcgagtgca gacgggcaag tgtcggagaa gacaaactat gaagagtggc tcagtagcaa
     2881 aacaccggaa gagcaagata aagcgctagg caaaggcaag gcggatttgt ggcggcgggg
     2941 agtgataacg tttagcgata tgttagatca aagtgctaga ccattgacac taaaagagct
     3001 atattcatta aaaaatagtt cttcggtaga taaaatggat agtgtggaac ttgtccgtaa
     3061 aaaggctgtt gcagttgagc ctatgattac tcgtgatgta gtatcaatta ttacagcatc
     3121 tgggggcgag cctgccggct tagattttcg cttgaaatca ctatcatctt tgcagcgaaa
     3181 aattgatacg gaaataatgg ccggagtgtc aaaagagcaa gcgatagcaa gtattcgaga
     3241 tgttattcgc tacactgcaa tcttagatga acagcgtttt gttgagcaat accaaaaaat
     3301 gcaaaaagac ttggagaaac aaggttattc tacgatcatt gttaaaaaca cttggaaatc
     3361 aaataatgct tataagggca ttaatacgtt tgtgagcact tttattgaaa agaataatat
     3421 aatttttgaa ttgcaatatc atacaaaaca aagttttgag ttgaaaaacg gtaaattgca
     3481 cgagctctat gaaaaattca gagatctgaa tatacctctt gcaaaaaaat cagaaatttt
     3541 acttgaaatg cagaagttaa gtgctaatct aaaagagcca aagaacattg atttaatcaa
     3601 ggagaaaaaa taatgtatca gtattattta gctcaagttg gcaatgaaca gcaaaaactt
     3661 attcgtggta tgcctgacaa tctacttagt ttctcggttt atgagcctga acaagaagat
     3721 tgggataata agttcggccc attttgggca gacaagatct tgataagcgg atttgacgct
     3781 tacgaggaaa tttcagaaaa ggaagcaatc cgatttatca aggtacatta aatgtgtcaa
     3841 gctgaacaat tcgctcgcca actgcattat ggacaaacag ataaagctgg aaagccatac
     3901 attgagcatc ttgcttttgt tgcagaaaac attagcacaa aaaatgaaga gacagaagca
     3961 gtagcttggt tgcacgacag tgtagaagat actcagtgtt caattaatga tattcgtcat
     4021 atatttggcg atgttattgc tgatgcagtt gaggctatta ctaaaaagca aggcgaaagt
     4081 tatttttgct acttggatag agtaaaatca aatcctattg ctaaggtggt taagttagct
     4141 gatttgactc ataatctttg ccttgagcga ttgcccaaga tcactgaaaa ggatcttaaa
     4201 cgagcagaaa aatacaaatc agcattggaa tatctacaag cctagcctaa aacgctaggc
     4261 ttttttatcg caatttttta ccccaaactg accgtttgtg agccatcaca ggcggttttt
     4321 tattatcggg cggaagccca ccagcaatga gaggaagctc aaatgaaatt aaaattagac
     4381 gaaaacggac atgtagtagt tgaaaatggt atgcctgtgt atattcacga tgacggaaag
     4441 gaaattccgt tcgatgcact aaaggcgaca cagaaaatca cacaactgaa cgctgaggca
     4501 aagcaacatc gagaagctaa agagaaagcg gaagccgact taaaagcatt ctcagggatt
     4561 gacgatccga aagctgcact cgaggcatta aagacggttg ccaatttaga tgctaaaaag
     4621 ctgattgatg ccggtgatgc ggaaaaagtc aaagcggaaa tcatcaaagg ttacgatgag
     4681 aaattagctg tggcaaccgc tcaagccgaa aaactgcaac agcagttaca cgctgaatta
     4741 atcggtggtt cgtttgctcg gtctaaatac gctcaagaac acttgaatat tccgtctgat
     4801 gtcgtacagg cattcttcgg taagcatttt agtatttcgg atgacggcaa agtggtagct
     4861 aaatttgctg acgggggcga aatctttagt cgcacccgcc cgggcgaaaa agcagatttt
     4921 gaagaggcat tagaagcttt agtaagtgct tatccaaata aagacgctat tttaaaacca
     4981 agtggttcaa gtggggcagg agcaggtggc ggatcacgca gttcatctat gccgaaaaca
     5041 ttatcggact gcaaaacaga tgaagagcgt atcgcttatc ttcaagcaaa ttcgtaaggt
     5101 gcaagaggtt gcaccttttt tattttcggt gcaatcgcac cactttttag aggaaaatca
     5161 aaatggcatt tgacctacag gtttttaaca aacaaacgca attagccttg accgaaactg
     5221 tcgatcaggc tattgaaaaa ttcaatgcag catcaggcgg tgcaatcatt ttacagaaca
     5281 agccagctga aggtgatttt gatatccgtg ctagctttaa aggtattgcc ggtttagttc
     5341 gccgccgtaa tgtgtacggt acaggtacgg tagatgcaaa acgtttagag caattactgg
     5401 acgtagcagt taaagtggcg gcaggtacag caccgattga ataccagccg agtcaatata
     5461 aatggatttt attaaatcct gaattggcgg cgattgaaat cggcacacaa cttgccaaag
     5521 cccgtgttgc tgatatgctc aatacggcta ttgccggcac ggttgctgca attagtggca
     5581 atacagcaat gacacttgat gaaaagaccg ccgctccgac attccgcatt ttaaacaaag
     5641 gtgcagcgaa aatgggtgat cgtgccggtt cgcttaaagc gtgggtgttg cactccacaa
     5701 ccttacacgg cttatacgat aatgccttaa ccaatgcgga aaacttattc aagtacgaaa
     5761 acgtgaacgt aattcgtgac ccgtttgggc gtgtatttgt cgtcacagac agcccggctt
     5821 taatggcttc agacggtaca tttaacacct taggcttggt tgaaaatgcg gtggtagttt
     5881 ccggcaacaa tgactttgac agcgtgttag tgccgaaaac cggcggagaa aacctttcag
     5941 cagtttacca agctgaatgg acctataact taggaatcct aggttataaa tgggatatga
     6001 ccgctggtgg taaatcaccg aacgataccg ccctaggcac accgacgaac tgggataaat
     6061 cggttacctc caacaaagac accgccggtg tattggtaaa aacgaagtaa tttttaaccg
     6121 cttgtagata aggtttacaa gcggttgttt ttttatctgg agtttgcaaa tgaaacgaat
     6181 actttatttt acaacggatt tttcggcaaa aaacattgcc tttgccaaag ctcacggtgt
     6241 attaatgcgg aatctgaacg cttaccaccc atcagacacc ttagaaaatg cagatgcagt
     6301 gtgcggtaac gtacctcaag cttatcaaca tttaccgttg tttgaattgc ctgagtctaa
     6361 ccaaaaatca ggtcaagatt tgaaaacctt aaaaaccgaa gaaattaagg aaaaactgac
     6421 tgaattaggt attgcttttg aggcgaatgc caaaaaagaa acattactaa ccttactcac
     6481 tcaggcggtg ggacgtgacg gaacaaacga tgctaaatag ttatatgacg gttgagcaag
     6541 ccaacgccta tcacgacatc agaatgagcc gtgaggcgtg gaatgcgtta agcgatcaag
     6601 aaaaacagca acgattggtg tcagcgtctg attttctgga tttcaattat cgtttttcag
     6661 gcgaaaaagc agatcttact caagagcgtg aatttccacg caagggtttt gaagctatcg
     6721 gtattcctaa agccgtgcaa tttgcggtat gtgagttggc attgcaagat aacttgaccg
     6781 caaatcaaga agctcaaatg agtagcgtaa gagttggccc ggtttcagtt aattatgata
     6841 acagttcacc gcaaaacagc gtgaatcggt ttgagtatgt gaggcagttg ctttccactt
     6901 ggttagatcg atcttctttc ggcacggtta agttggagcg tagttgatgg cggattttta
     6961 cggcaattta gggaaaatct cccattcttt gattaagcaa ttcgggcagc cttgtagtgt
     7021 aacaattaac gaacaaggca gatacaaccc tgcaaccggt gagcggaaaa aaggtagctc
     7081 aacggttcac gcaggctatt gtctgtttga taatttggcg tttgatttcc gcaatcagca
     7141 atctgtcagc gttcaacaag gcgatgtgat gatttatctc actgccgatt gcaaaccggt
     7201 attaaatgct gtgattgaag caaacggcga aaaatggtcg gtcatcaacg tccagcctat
     7261 acgcccagca gctaccacga tgatttatca agtacaaggg agaaaagccg atgggtagtt
     7321 ttcacgctca aatcgcccgt tttgtggaac aaacgcaaca aaaagcggat agggcgttaa
     7381 gggtaattgc cctgcaaacc ttaagtgggg tgcagaagaa atcgcctgtc gataccggac
     7441 aacttcggcg tagctggacg gtggcattaa atggtttccc gagcagttat aacggcagtc
     7501 aaaccgtgct gaatagtgct aaattcggcg atatgattgt gattgctacc aataaacctt
     7561 atgcaccaat gctcgaatat gggctttacc cgaacccacc aaaaaaaccg accggcaaga
     7621 ccaaaaacgg ttattcaata caagcaccta atggaatggt gcgaattacc gtgcaggaaa
     7681 tgcaagcctt tatccgcaat aatccacaat taggagtaag ctgatgcgac cgattatccg
     7741 ctcggtatta gagcaacatc ttgccgaact caatccgttt aatacggttt gggagggcat
     7801 catcgacagc ccgaaactgc cgtatcaaac cgtatcaatg acgacttcaa ccaacgaaac
     7861 cgccacgatt ggtaatacgc cgaaagccaa acagagcggt tttttgcagg tcacgcttta
     7921 ctatccgcta gatcagggga caaaggcgat tgaggaacga gccgatgaga tcttccgcca
     7981 ctttttcggg caggtctgga ttgaggaaaa tgtgcaaatt ctggtgcaac agccaccgct
     8041 tgtagcctca ctttatcgac tggataacag cattgcctta cccgtcacca tctattttac
     8101 cgcttatgaa ctataggaga acccaatgtc tattctaggc aattcacaag gcacaaaacg
     8161 tacggtcgtt tttgccaaag agagttcttt cggcacgaaa gctgctccga caggcgcaaa
     8221 aattatgcct cgtgttgaaa cctcactcaa taccaatttt gaaagttatc aatcggaaga
     8281 gatcagagat gatttacaac gctccgcctc tgtgatcggc tttgaaaagg tcgaaggcga
     8341 tattaaaggc gaactagccg ccggacaatg ggcggcattt tttgcggctg cattacgtgg
     8401 ttcatttacc gctcaggcga aaaagccgat tattaccaaa accacagacg gcaccggcga
     8461 gaaaaacggt aaaattttga ttgtgccgaa aaccagccac acctccgaca gtttcacgct
     8521 ggaagatgtc ttcaaagaca tcaacgtcag ccgccagtat ttgggatgtc gtgtatcgaa
     8581 actcagcctt gatgtgcaac caaacggtat tgcctcgatt acagtctcat ttttagggca
     8641 acgaggcgaa gaaagtacaa cagcctattt taccaatccc accgatttaa cccaatccgg
     8701 caagctggca ggggtaaaag gctcattgca ggtcaataaa cgcccagtcg ggttagtgac
     8761 ggcctttaaa ctggacattg atttaggggc aagcagtgag ccggtattag gggcaaaata
     8821 cgcccctgat gtctttatcg gtacagtggc agtgagcgga tcgtttacga tgtacttcca
     8881 agacaagacg atgattgatg cggtgcgtaa cggcacagat ttatcccttg ctttgcggat
     8941 ggatgccgaa gagcgtacca ataccgacta tttaaccatt atcttaccgg gtgtcaaagt
     9001 gacctcaaac gagttggacg atggggcgaa aaacattatg cagacactca attttgacgc
     9061 attccctgcg gtatgggatt cagcaagcca aatcgacaac gtgctaaaag tcgccaccac
     9121 aatgattgtg caagacagcc tcgcttaatc gaaattttgc aaaaaaataa ccgtatctaa
     9181 ccgcttgccc tcaaacaagc ggtctttttt atccccaatt tacaaaagga aacccaaatg
     9241 aatttaaacg cattaagtaa agaaaaacag ttagccgaac accgttttga gctggttcac
     9301 cctgttagtg gcgaaccgct cggcgtgttt atgagcgtcg tgagtgctaa gagtgataag
     9361 gctcaacgct ttgccgaaaa acaattacgc caagaacaga tgagagagct ggaaaatgcc
     9421 aaatcccgca agccgaaatt caaaggctta ggcgatatga ttgccgaaag tcgtgcaatg
     9481 gcgattgacc gcttaaccgg ctgggagaat gtcgaatgga acgaaaaacc gttggagttt
     9541 tccgaccaga atgcggagct acttttaacc gaatgtgatt ggattgtgga gcaaatcttt
     9601 gagcattcga acgatctggg aaagttcttg aagagctaat tgatgattta caggtgtatg
     9661 cagagaaaac gtttaagttg gataaaaaac ttaaaggcag cgaaagtacc ttgcgagagc
     9721 atttgcaggc aatcgaaatg caaactggca ttacaccaga agaattgcat aaccctgaac
     9781 ccagtccagc ggtggcctat ctgctgggct ggttcttcga gttggcaatg agccgacaat
     9841 cgggtatgag cttaaaccca attagctaca ctgaaattga tgcgtggagc aggctttttg
     9901 ctcgtcatct cgaacaatgg gagatcagcg taattaagca gcttgatatg atttacttga
     9961 acgtacaaca aacggagtag tatggatact ttcaactttc agcccaattg ggggcttaac
    10021 gtacagagaa agccagaagt gatgaaaatc gcctttggtg acggttacga acaagtcgcc
    10081 cctaaagggt taaaccataa tctccgcact tattccgctg ttttcaccgg cacagaagca
    10141 cggattaaac agattgaggc attttttgaa cgacaaggcg gctataaggc ttttctttgg
    10201 acaccttacg gcgggaaaga ggggaaattc cgctgtgaag agtgggaggt aagcataagc
    10261 agcgggaaat ggacgctgtc ggcgacgata agagaagtgt tgtagttttt tctgattttg
    10321 tgaactggtt cacactctgt gggttggata taggttagaa tttaagtcta atttgcttaa
    10381 cggagtcttt tatgaaaaaa atttttgttt cagccttatt gctcacttgc tcgttatctg
    10441 cacttggaaa ttcctcgtat gagttgatta ttgactcaat gcaagatctt gaaaagcata
    10501 aacccgaaat tctatctata ccagaagcgg atagaaatac ttttattcgt tatatgatgc
    10561 gaaaagaact gcggataaag ttaaatcaag gcagattagc ggatggtcta actgtgagag
    10621 aagctattca aaatcaacag gcatttgaaa aagatagaaa tagcaaaatg cagcaggaga
    10681 agcaaaaaaa agaaacagag aaagcagaaa aagaaaaagc tgttacggat tttaaccaaa
    10741 aatttgagct atccattaca ggggcgagaa aggcaaaaaa tgcactaaat caagatattt
    10801 tgcttgtttc attaagtcta aaaaataact cagataagcc aattatcgct gtacaaccgg
    10861 atattcaatt tgtactgggt gaagataaac aaactatttc atccatgcgt ccaaaaaaat
    10921 ttgaaaacgg cattccgcct aatggctcag agatttttga gtttgggttt gatttagatg
    10981 atatgtttgc tttgaaagca gaaaaaaata tagagaaatt gagggcagtc ttccaaacag
    11041 cagaagttct ttttgctgat ggaacaactg aaaaacttca atagtaattg gtaaatcttc
    11101 attggccgct tgtagaaata caggcggttt ttttattgga gcaagctatg gcagatttag
    11161 caacactggc gattaaaatc gaaacagaag gcgcaagccg agcaagtgca gatctaagta
    11221 aggtcgagca atctgcaaag aacacagaga aagccgcaga taatctgacg cgcgcaatgg
    11281 gaaatttgaa gcggatttta gcgattgcag ggctggctgg tgggttatcc gcttacctag
    11341 atatggctga aagaatgcag tcgttaaacg cacagcttaa atttgttact aactctcaat
    11401 acgaattcaa tagcgcacaa aaagagcttt ttgcgattgc gcagaatacg cgcgctagtt
    11461 tggaaagcac cacgcaactt tatattaaat cttctcaggc actgaaagat tacggctacg
    11521 cgcaaaagga tattctcaca tttaccgaaa ccattaacaa agcaatggca gttggcgggg
    11581 ttaagccaca agagcaagct agtgctttac tccaattatc acaggcatta ggttcaggga
    11641 aactgcaagg agatgaattc cgttcaattt cagaagctgc accgattatt ttagatacgc
    11701 ttgccgagta tatgggtaaa agtcgcgatg aagtaagaaa actcgcatcc gagggtaagc
    11761 tcaccgcaga cttactgttc aatgcgttta acggttcaag cgctaaaatt accgaaaaat
    11821 ttaatcaaat gccgctaaca tttggcggtg caatgcagca gttgcagaat gccacattga
    11881 aatttatcgg tgacctagat agtgcaaatg gcttgagtgg aatgttagct caagccatta
    11941 gttttttagc acagaatttt gatgtgttag gcaaggcttt gatttatgca accggtgctt
    12001 atattgcgtt taatgcagtt gcctttgctc agaattttgc aaaagcaacc ggtggtgtcg
    12061 gtttattaag tagtgcattt ggttatttga ctacgatgat ccgaggtgca actgttgcaa
    12121 tgatggctaa tcctatcgag cttttaacaa ctgcaattat tggggctgct tttgtatttg
    12181 atgcttttat cagtgatata gaggtaggtg cagcaacctt tgatgcgact tggggagatg
    12241 ttgcgacagc tgtgtgggat gattttaaag atttaacttc agatgcggca gattggttag
    12301 tcactcaatg ggataaagca accgccagtg cagcgagttc gttcagttcc ttaggtgttg
    12361 atattggctc agtatgggag acggtgcaat ccattacgaa aacattgatc aatagtatta
    12421 ttggtctgtt tgttggagct tatcaagcca tcgtgcttgc ttggaataat tttcctgcca
    12481 caatggaaaa cttgggtgtg atggcggtaa atgctcttgt cgatttggtt caaaaaggca
    12541 tcaatagcat tattcagctt atcaaattac cgattgagct tttaaacaga gcctcatcta
    12601 cttttggtgg aggaaatctg attgatacat caggttggaa ggcaagctta gatgatttta
    12661 aactaaaggc aagccgcgag gcagaagttg ttggtcaatc aattagccaa gcatttgcgg
    12721 atagtatcac cacagattac ataggcaatg cagttaattc agtgagtgac tatattgatg
    12781 gagcggcagt tcgtgggcgc ttaaaacgtc aattagatga aaccgcctct gacgatcaag
    12841 gtacgcagtc accagctaaa aaaacattag ctggaaataa taactctgct agtgactgga
    12901 aaaactacct caatgaactt gaacgagcca atgccgatgg attagcccgt attgcacttg
    12961 agcaagatcg ctcaatgcgt gaaatgttgg aaaaggcgaa gaaagccggt gcaagtcacg
    13021 ctgaaattga aaaagccaaa atgctgatta cagaacgtta tgctaaagag cgaatggaaa
    13081 ttgccgagaa gtatgtgccg agtctgaagt ttgaggaaga actcaaagat cagattaagg
    13141 aaattgatga actgcaaaag ttaaacctga ttaccaccga gcaagcaggt cttgcgaaag
    13201 aggcgttagc aggcaaatat cagccggtag ttgaaatcca acagcaatac atcaatcaac
    13261 tgagagagat tgaagcatta aaacaagccg gtgtattaag caaacctcaa gcagaagtag
    13321 cggccgaaaa agccaaatgg gacgccggca cacaaaaagc caaaatcgca ggtaaaaacg
    13381 ccgttagccc ttatgaaaaa tggaaatcgg aatttgaccc aatgcaggca attcaaaatg
    13441 agcaagcctc aaagttggca gaagtgcaat cgatgtacga tcagcatttg atccaatatg
    13501 aggattttat caacgctaaa gcacaaattg atgctaaggc gactgctgat actcatcaac
    13561 tgtttatgaa cagtattagt gggtttggtt cggcgataga tacgatgtta ggcgtgatga
    13621 aaaacgcagg gcaagagcaa tcaggtattt accgcacgat gtttgcgatg tcgaaagcgt
    13681 ttgctattgc ggaatcaatt ctgaacttga atcgtgcaat tactcaagca atgagcgatc
    13741 aaacagctat tacaccggct caaaaatttg caaatatggc ggctattgct agtgccgggg
    13801 cgagcttgat ttctaatatc cagtcagtct cattaggcgg tatggctcac agcggtattg
    13861 acaatgtgcc gaaagaaggc acttggttgc tggataaagg tgagcgtgta gtggatagtc
    13921 gtactaacca agacttaaaa acctttttat ccaatgctaa tcgtggcggt gcaagtggtc
    13981 aaagatcggc gataaatgtc aaaattatta ataacgggca acccgtagag gcgaaagtca
    14041 gcagtgaaga gactgtagat ggcaatatcc aaatgacggt ggaactgctg aagaaaatgg
    14101 atcaaatcgc cgatcaacgc taccgtagaa atcagttaaa tgatttaaga acaggtggaa
    14161 cattatctaa ataggtgaag tatgccaatt agtatttcta accaaatgaa actagatctt
    14221 gccaaacttg agcaaaatgc aatgctggac ttgtacgagg ttgatttgcg caatctcaaa
    14281 gataaaagtg gtaatgctgg cagagtttac cgcttttatt ctgggctaaa tgaactcaaa
    14341 acaagtatcg tctggcaagg gcggacttac gatccgtatc ctattgaggc aagtggattt
    14401 gaacgaaaca gtagcggccc gagcaatcgc ccgactctca cgctatcaaa tctgtttggt
    14461 ttaattacca gtattgcgaa tcagtttgac gaatgtatcg gtgcgattgt ccgccggcat
    14521 caggtttatg ctcaatattt agatgccgtg aattttgccg ggggtaatgc gaaagctgat
    14581 ccaaatcagg aaataatcag tcattttgtg attgagcagc ttaccagcct cacccgagaa
    14641 acggcgacct ttacgctggc attaccgatt gaaaccgata atgccaagat ccccagcaga
    14701 attattatgg cggacacctg cacttggatt taccgctcgg cggaatgtgg ctacaccggc
    14761 aagcagtatt ttgatgaaaa agacaaatca acctcagacc caaaagcaga taaatgtagt
    14821 cattgcctta atggttgtga attaagaggc aatcaacgca attttggcgg gtttgtatcg
    14881 gtaaataaat tggggtaatg aatggaattg gaacaacaaa taatcgacta cgccctccaa
    14941 cacgaaccgc acgaaatgtg cggctttgtt gtttttgacg gcaataaaaa tcgatttatc
    15001 ccttgcgaaa accaagcgga agacaaagcc aattactttg aaatatccga tcttgattac
    15061 atcaaagcag aagaaaaagg cgaactggta gcggtggtac attcacatcc cgagccaaac
    15121 ggcaagctta tattatctgt gcttgatcgc aaaatgcagg tacagaccgg tttagattgg
    15181 tggctagtgc ataatcggca aatccataaa ttcaggaatg tgccgcattt aatcgggcgt
    15241 gagtttaaac acggtacaat ggattgttat acgctctatc gtgatgccta tatgcttgcc
    15301 ggttatgaga tggacgagtt cgagcggcaa gatgactggt ggcatagcgg gcaaaatctc
    15361 tatttggata atattcaagg gcaaggtttt gagcgagtag aaaccccaca aatcggcgat
    15421 gtgattttaa tgcaggttgg agctgatgta cctaaccacg ctgccattta cattggcgaa
    15481 cagatggtaa tccatcatag cccgaaccga ttatctaagc gtgatttata cgacggctat
    15541 tggttacgtc atacccacag catttggcgg cataaactgg cggagaagtt agattttgat
    15601 ggcatattga atgatattgc tgttcaggat tagcaaaaaa tctttaaagc agtttaaaag
    15661 aaatttagcc attaagttgt gaaaatacat ttgacaaagc aaaggtgtgg aagtccttcc
    15721 tcattacaca gacaatgagc attgttatga tttcacaacg caatcggaaa aaacaaaccc
    15781 cgaagcgttt gcagcacttc ggggtttttc atatccaaat acctaataaa aaggaatgta
    15841 tatatacaat ggaaattatt gcatttattg tcacttcaat caaggagatt atcacaatgt
    15901 ttgaagaatt atcaacgtgg cgatttatcg ctattttaat cacgatcatc atctgttttg
    15961 ctgtgtggaa atcgcctgaa atcgtaaaag tactttacaa caagaaaagt tcagagtagt
    16021 atttacccca taggtgtcga aacctacata aaaggcggta atccgcaacc gaaaccccta
    16081 tgcggttttt ttgtacccga aatttggggc agaaaaggtc tattaaattt ttgtctatga
    16141 tcgagtgggt ggcgaataca acacccgaaa gggaaataag cccagccgac cttttacggc
    16201 tttcgaacca cttgatcgcc ctaatctacg gattaggaaa tgtcgaaaat ttaaaaggat
    16261 ctcactatga ctactcaaac aaaactctca acattcaact ttgaaaccca acaaatccgt
    16321 gttatcgcag taaatggcga accttggttc gttgcaaaag atctctgtga tacattggga
    16381 atttcaaatc caagcaaagc aattcttaac ttagatgacg atgaaaaaat gatttcttcc
    16441 gactctaact taaagttagg gtcggcagga aatggtgctc aaagtctaag tttagtttcc
    16501 gaaagcggaa tgtacaccct aatcctccgc tgtcgtgaag cggtgaaaaa aggttcagtg
    16561 ccacaccgtt tcagaaaatg ggtaaccgcc gaagtgttac cgcagatccg aaaaacaggt
    16621 gcttatacca aacctgttga gcaagagccg aaatttaccc atactctaac aagcgatgaa
    16681 ctacgggatt tagcgtggct ctggttcgag gcaagcaaaa tggcggaaac aatgaacacg
    16741 ctttatcaac cgctgatggc tattggctct aatcttgccc cgatggtgta tagccaaggt
    16801 gtggaatacg gcaaccatat tcagcgaatg ggcaaactca tctaccgcct cacctatggc
    16861 gtagatttta gcaataatcc gacaccgttc cgccatttac agcaaggcgt tgcactaccg
    16921 caccggtaat tcgcaaactt ttacaaaaaa atgaccgctt gcaggaaact gcaagtggct
    16981 tttggcatac attaaattta ttggagcaaa tatggtgaaa gtcaaatttt acggacacct
    17041 gagaaaattc ggtacagact ttaagcttga ggtaaaagac accgctgagg caattcgtgc
    17101 cttatgtaca cagattagag gattgcgtga ggcattgcga gacggtgttt ataaagtccg
    17161 tatcggtaag caatatttag acccatcagc ccttgaaaaa gggctttttt attgcttgaa
    17221 gaaagggcaa accattcatt ttacccctgt tatcaaaggg gcgaaaagcg ggggtgtttt
    17281 ccaagctgtg ttaggtgtag ccttaattgg agccgcattt tttctcgggc cggtcggttg
    17341 gggagttcta ggggcttctt ccgcaatgat gatgggagca atgggggcat caatgttact
    17401 aggaggagtt tcccaaatgc tcaccaaaat gccaaaagcc cccacaatgg gtaatgaaac
    17461 agaaaaagaa agctcaacgg ctttttcaaa tttaaataac ttggtggctc aaggcaaacc
    17521 tgtgccgttg gcttatggct taattcggac cggctcgctt gtgatttcac agggagttga
    17581 gacgatgact gtagaggtat aattatggga ttatttagtc gtaagagaaa aaatcatact
    17641 cctaaagaag caccggaaac tggtcgctct aaacagattg tgaatatcgt ggagttactc
    17701 tgcgagggtg aaattgaagg cttggtagat ggctttaaat cgatttatct tgacggcacg
    17761 aaaatccaaa acgatgacga cagctataat tttaataatg tgagcggtca gttaaatgtc
    17821 ggtacgcaag atcaagaggt attagaaggt tacgacagct cacaaaatga agtgagtgtg
    17881 ggtgtagaag tcaagaaaaa aaacggtgca atcgtccgca ccgttactga tgaacgcatc
    17941 agccgtttgc gtttaacgtt gggcgtgcgg tcactattcc atcaaaataa tcaaggcgat
    18001 accaatacca ccaatgttga tttaaaaatc acgattggca cacggcaata ttcgcatagc
    18061 tttaacggca aatacagctc gcaatacctt gaatctgtgg tgtttgataa cctgccgcct
    18121 gtgccattta atatctcagt tgagcgtcta accgaggata gcaattcgca acgcttacaa
    18181 aacggtacaa tttggagtag ttacaccgaa attatcgata ccgagtttac ctaccctaat
    18241 tcagcggttg ccggtatcag ttttgattcg gagtatttta ataatatccc gacccgaaac
    18301 tacctaatta aagcgaaaaa agtcaaagtg ccaagcaatt atgatccagt taaacgcact
    18361 tacaccggct tttgggacgg cacatttaaa attgcgtgga cgaacaaccc tgcttgggaa
    18421 atttacgatt tagctccgat tttaagcaaa atgcttgggg ttgaaatcag ttttgacaaa
    18481 tgggcgttgt atgatgttgc ccgctattgc gatcaacttg tgccggacgg tatgggtgga
    18541 atggagcctc gtttcacctg taacgtatgg ctcacggaag ttaaaactgc ctatgacttg
    18601 ttgaatgatt tctgctcggt attccgtgcg attccgattt ggaccggaac agaagtatcg
    18661 gtcattatcg accgtccgcg tgatccggtt tggacttaca caaatgctaa tgtagttggt
    18721 ggatttgaac gatcctactc tgctcgcaaa tcacgccata atgcagtgca agttacttat
    18781 tcggataaaa caaatggcta tgaaagtgcg attgaatatg tctctgatga cgaggaaatc
    18841 aaaaagcacg gcttaaattt aagccaagta actgcctttg gctgtacatc tcgtgggcaa
    18901 gcataccgta ccggcaaatg gattttagaa accgaaaaac gtgaaaaaga aaccatcact
    18961 tttaccgtgg ggcgtgaagg cttaatgcac ttgccgggcg atattattcg tgtggcagac
    19021 agccattatg ccggtacgga aattggcgga cgtgtactgg cgattaatgg gcgaaaagtc
    19081 acgttagatc gtgaaattag cattgataac gccagttatt ttacctatat caacggtgag
    19141 gcaacgcact ctagcattaa aatccaatcg gtaaacggta aagaaatcac gctagatagc
    19201 acgccaaccg gcttagaaac ctacggcgta tggtcgttat cgactaaaca aatcagctcg
    19261 ggcttatacc gctccatctc gattgttgaa aatgcggacg gtacgaatac gattactgca
    19321 ttgcaacacg aaccgcagaa agaagcgatt gtagataacg ctgctcactt tgtggaaacg
    19381 gcgagaacgc tttacaaagc cccgcaaatt aacgcagtag aagtctcgac tggttatgac
    19441 ggcaagctct atatctccag tgatattagt tctggcgacg gcaagctgac ctatgatatt
    19501 aaaatcacca aagatggcaa tttttatcaa ttcaagaaag gattagccga cccaaatatt
    19561 gagttgagcg atttaccgaa tggcgattac agcgtgatta tttacggtaa aaatgccaaa
    19621 gggcaaatcg tcaccgaaaa aacacaaacc tttactattg atcgacctcc agctccgacc
    19681 ggtgtagtag taacaggtgg attagggcaa attaccctcg aatgggattg ggtaaatgag
    19741 gttacgcaaa ccgagatttt cgcagcggaa actgataatt tcgcccttgc aaaaaaaatc
    19801 gcaaaagtga ccgctagaac ctacgctcac acactaaaag gtaacaaggt tgtacgctat
    19861 tattggctaa gacatgctag aggcattaat gtcggaccgt tttatcaaca acagggggta
    19921 agaggcgaaa gtgcggttga tttggatgct cgattaaccg aattaaacac tcagctatca
    19981 cgcaatattg ttaatgaggt ttttgatgtt gctgcaccgg cacgaggttt agagctggt
//
LOCUS       DANLXF010000012        19762 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.012, whole genome shotgun sequence.
ACCESSION   DANLXF010000012 DANLXF010000000
VERSION     DANLXF010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 19762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19762
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.012"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            46..930
                     /gene="oxyR"
                     /locus_tag="PXD66_000271"
     CDS             46..930
                     /gene="oxyR"
                     /locus_tag="PXD66_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HDL6318606.1"
                     /translation="MNIRDLEYLIALAEHKHFRRAAESCNVSQPTLSGQIRKLEGELG
                     TILLERTSRKVLFTQAGLTLVEQAKVVLREVKILKEMASNQGKEMSGPLHVGVIPTLG
                     PYISPIIVPALKAEFADLELYIYELQTNQLLDQLESGQLDCGIISFVKESESFIEVPI
                     FNEEMYLVVPEQHEWAALKKFDMNLLKNRELLFLDSGHCLRTNTLDYCLSIGAKENPH
                     FKATNLETLRNMVAANVGLALIPKLAAKPIKGVHYIPLENPKPFRAVGMVYRPGSPLR
                     ARYERLAQAITKIMQSEA"
     gene            933..1565
                     /gene="fabR"
                     /locus_tag="PXD66_000272"
     CDS             933..1565
                     /gene="fabR"
                     /locus_tag="PXD66_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HDL6318607.1"
                     /translation="MANGMSVRAIQKEKTRRALVEAAFNQLSSEKNFSNLSLREVARE
                     AGVAPTSFYRHFKDMDELGLAMVDESGLLLRQLMRQARKRIANGGSVVVVSVETFFEL
                     IADRPNVFQLLLRESSGTSQAFRTAASREIQHFVAELADYIMSKDNHSNRELAYIQSE
                     GLVTLVFTAGSHALDMNNQEREKLKQRVIMQLRMLIRGSTYYANKQASST"
     gene            complement(1592..1891)
                     /locus_tag="PXD66_000273"
     CDS             complement(1592..1891)
                     /locus_tag="PXD66_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HDL6318608.1"
                     /translation="MKKADIQKMQDCYNQWVELLPELEKGIEQWKRASALLEPLSRFY
                     ASPEWRELHDSFDEQLDTKGNYSILSEDALWNALSEHHQLALEWLKLSTAYITKE"
     gene            complement(1980..5795)
                     /locus_tag="PXD66_000274"
     CDS             complement(1980..5795)
                     /locus_tag="PXD66_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HDL6318609.1"
                     /translation="MKGSGLATVGAPETGDNGQTIYTVDVAKAGAPTVTRGNVADAIN
                     NSEKTSSVVAGSKAVTVKAGKEDDKGNTEYTVDVATDKSIGRDNDGNMTVNTDNVTIV
                     KDPTTGEVKANTTTLNNSPEGKVTEPTGDDAKKLVTAGDIANAINNSGFNVTAGDGVD
                     GGETKGKKTQLIKPSETVTFDAGKNMTLTQADGKFTYTTKDNVAFNSIDMSNGKPDTT
                     GSITNLKSGVGGTFADKAAPTDAERKAIADNINNATGDTLNNAVNVGDVQAAMKAATT
                     KVEGDKGVTVTSKTNDDGSTTYTVEAKTDGTTIKVNDKGEITANTSELTNNPDGKVEE
                     PTEPNALVTAKTVADAINNAGFNIKANGDEKSLVKTGDTVQFLNGKNIEITRDGNNIT
                     VSTAKNVNFDSVQFGNEGPKITNNGGNINVGDKDGNAVKVTNVAAGDVNADSKDAVNG
                     SQLYTFAMASREEVKSTDKSVTVNTTKNADGANVFDLSVNTDDVTIVKDPTTGAIKAN
                     TTALNDANNDGRIDEPTADDAKKLVTAGDITNAINNSGFTLKTSAVEGGEKLSGGDEL
                     INPGKAVEMVAGKNLTVKQEADGKVIYATKDDVKFSSVTSNTVTVPTDEADPANNPIT
                     INKDGINAGNKAISNVASNLIPVTADDKVQPADNNPTNLADKLSNAATLGDVLNAGWN
                     LQGNGKAVDTVVHNDTVDFINGKGTTVTVENKDGKNTIKVDSPIEFVNQDPTDSSTPS
                     NTAKFTGEAPVQLGNVASSVRNEDGSTPEGKDRAEAIKNAEGDKLNNVVNLGDLQAAT
                     NAATTKVGGNRGVTITPSTNADGSTTYNVEAKTDGTTIKVDNEGNITANTSELGNNED
                     GTVKAPTQPNALLIAQTVADAVNNAGFNIKSAGNKAAGDQAATKLVKTGEEVVFEAGD
                     NLTVKRDGNQFTFATAKDVSFNSVQFSENGPKITNDGDNIKVGDKDGKPTKITNVADG
                     DISPVSTDVINGKQLNNYAKVNGNNIGTDEDGSINIVNGNGTTITSDKAGEVKVNVNI
                     TDLTVADNGKINVQDPNGTGSRFVNATTVANAVNNVSWNVDSKAVGTGAVEGDKAPAK
                     VKAGSTVSVNAGNNIKVTRKGSDVTVAVSDTPEFTSKTGDTLVNNNGVTINNGSAGKA
                     VSLTKDGLNNGGNRITNVKAGEADTDAVNVGQLKGAVNHLNNKIHRNNREARAGIAGS
                     NAAAALPQVYIPGKSMVAAAGGTFKGENALAVGYSRSSDNGKLILKLQGNANSRGDFG
                     GGVGVGYQW"
     gene            complement(5758..6243)
                     /locus_tag="PXD66_000275"
     CDS             complement(5758..6243)
                     /locus_tag="PXD66_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318610.1"
                     /translation="MDTGSITVDNTTGAVTTPAEEDKVATTKTVSEAIQKAGWNAKSG
                     GNKADGDQEAAELINPGEKVIFAAGDNLKVKRVGTIFTYETAKDVKFDSVTFGDNGPK
                     ITNKDGNVNIAGNDGNPTKITGVKAGEADTDAVNVSQLKQAAASQNRSERFRFSNCWC
                     T"
     gene            complement(6252..8411)
                     /locus_tag="PXD66_000276"
     CDS             complement(6252..8411)
                     /locus_tag="PXD66_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL6318611.1"
                     /translation="MGARATSANAIALGTDTAATGNRATAFGAGALATGNRSTVMGWR
                     SAASGTRSFAMGSGAMGDKSVANATYSLALGSSANANATSTATRGTAIGYNATVSGVN
                     ATALGVSAVSSNQSTVAIGDSAKASAGFAIALGSGANVALNNSVALGSNSTVSNAVAV
                     PSATVNGITYSGFAANTTAAGNVVSVGSNTIKRQIQNVAAGQISATSTDAINSSQLYM
                     AMNATGNLANSTKNILGGNATVKPDGSVTYTNIGGTNKNTIEEALKAVKTEVVAGSNV
                     NITNATGANGQTIYTVNAYNTTANSSSPDYITVTGKAATAANTTNYEIGLTKKAIDDF
                     TKDTQATVVSNDGTVTVKSTERNANGTVIYDLSVNIPAQASQIQYFSVNSTVPENQAN
                     DGAKSRNSIAIGPNATATGGEQAAVALGTNSNANGNGALSLGVATVSKGIQATAVGHS
                     ANATANGTTALGRQTNATAGDATAVGSNANATAEKASAFGVAANASANASLAVGANSI
                     ASAQSAVAVGTRANATAQFATALGMGAQATLNSSVALGSESVVRAATPTENATVGGIT
                     YNGFAGVNKDTNYVVSVGSAGKERQIQNVAAGQISATSTDAINGSQLYMAMNATSNLA
                     NSTANNFGGGSVVNPDGSVTQPKYNVTNAAGTQYGNTATNVGDAITNLNNYVNQGFNI
                     KDNAGETKGTVTPNESVQFVNGKGTVSNVTQEADGVK"
     gene            complement(8640..8840)
                     /locus_tag="PXD66_000277"
     CDS             complement(8640..8840)
                     /locus_tag="PXD66_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR domain-containing protein"
                     /protein_id="HDL6318612.1"
                     /translation="MNQIFKVIWNQATQSWVAVSELQRAKGKTKSVSSASKATLALLA
                     GASAVLGANDAVAVVSYRFNNK"
     gene            9204..10358
                     /locus_tag="PXD66_000278"
     CDS             9204..10358
                     /locus_tag="PXD66_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HDL6318613.1"
                     /translation="MNQFFPLIFSLTLFSGVTLALEKAPRTMPTEGINKPLLGQILFF
                     DNMLSHNGSQNCSSCHNPDYAFIDNRTTPAKGVVSQGDTPTKFGKRNSPTMLYAKYSP
                     EFHFDEQSQEYIGGQFWDGRAKNLAEQAGMPPIDPNEMGMPNKLEVAKRLYNVPMYQR
                     FLGQFYGEKVWESYESVYAAMEDAIATFQQENKLLAPFDSKYDLFLKGEVQLTALEEK
                     GRALFFNKAQTNCSSCHQLQSAANHQEETFSNYHYYNVGVPGNPDLIAVNGFAPDFVD
                     LGLFENPNVKGDETQKGKFKTPTLRNVAVTALYMHNGVFKELKTVLLFLDSYNNPERK
                     INPETGTAWQQAEFEPTIAHQKLKAKPLSDEEIDALEAFLKTLTDERYRK"
     gene            complement(10406..11395)
                     /locus_tag="PXD66_000279"
     CDS             complement(10406..11395)
                     /locus_tag="PXD66_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HDL6318614.1"
                     /translation="MNDLIIYNTEDGKSNVALLVVENEAWLTQNQLAELFDTSVQNIS
                     EHIKNILKDKELDENSVIKDYLITAQDGKNYQVKHYSLEMILAIGFRVRSPRAVQFRK
                     WANSTLRTYLEKGFLIDSERLKNPQGRLDYFDELLEQIREIRASELRFYQKVRELFKL
                     SSDYDKTDKNTQMFFAETQNKLIYAITKHTAAELICQRADATKMNMGLTSWKGKVVRK
                     GDIVIAKNYLDNNELDSLNRLVMIFLESAELRVKNNQDLTLGFWRRNVDMLIEFNGFS
                     VLGNGGTITHKQMESFVREQYEKFDIQRKCLKQKEADWEDLQALEKLESELTR"
     gene            complement(11555..12919)
                     /gene="mpl"
                     /locus_tag="PXD66_000280"
     CDS             complement(11555..12919)
                     /gene="mpl"
                     /locus_tag="PXD66_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HDL6318615.1"
                     /translation="MKKHIHILGICGTFMGGVAMIARELGYKVTGSDTNVYPPMSTFL
                     AAHGIEIIPNYDVAQLQPAPDLVVIGNAMSRGNPCVEYVLDNQLNYTSGPQWLHDHLL
                     KDKWVLAVSGTHGKTTTTGMLAWILDQNGIDTGFLIGGVAGNFGISARAGNSPFFVIE
                     ADEYDSAFFDKRSKFVHYTPKTLIINNMEFDHADIFDDLKAIQRQFHHLVRTMPQSGC
                     ILSAIADQNVQDTLKMGSYSQLQFIGEQNEWFAKPLNADCSQFEVFHLGVKQGEVHWS
                     IIGKHNMHNALMAIAAAHHAGVEVQGACEALGKFVNANRRLEVKGEVNGITVYDDFAH
                     HPTAIEATIDALRGKVGKNQRILAVLEPRSNTMKMGVHKDEIAPSLHDAEAVFVYQPD
                     IIAWSVSEITNVLSQPAYWSANIDDLVDLVVKEAKPTDHILVMSNGAFGGIHQKLLAK
                     LADK"
     gene            complement(13215..13970)
                     /locus_tag="PXD66_000281"
     CDS             complement(13215..13970)
                     /locus_tag="PXD66_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HDL6318616.1"
                     /translation="MKLKSFKPMFMLLSVAFLTACSSSINPETGVRDDPLEGFNRAMW
                     KVNYDYLDPYVLKPVAKGWKEYVPSPVKTGLINVANNLDEPVSFVNRLIEGEGKKAMV
                     HFNRFWINTVFGLGGLIDWASLDEGLRLNNGQRGLGESLGAHGVPSGSYIMMPAYGAT
                     TPRQGIGSAVETGYSVLTYVGSPWSIAKFVVQGVDTRSKLLEQDALLEQAQDPYVTFR
                     EAYFQNLEFKVKDGNVEQSQQEQLSEEELKNID"
     gene            14125..14442
                     /gene="metJ"
                     /locus_tag="PXD66_000282"
     CDS             14125..14442
                     /gene="metJ"
                     /locus_tag="PXD66_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HDL6318617.1"
                     /translation="MADWNGEYISPYAEHGKKSEQVKKVTVSIPIKVLEILTNERTRR
                     QIKNLRHATNSELLCEAFLHAFTGQPLPNDEDLMKDRADEIPEAAKQKMCELGIDPDE
                     WQY"
     gene            14490..14720
                     /locus_tag="PXD66_000283"
     CDS             14490..14720
                     /locus_tag="PXD66_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF997 family protein"
                     /protein_id="HDL6318618.1"
                     /translation="MYQQLTTEARWAVLLTLIYLLGWVGFAYFSPQGRGIFGFPIWFE
                     LSCIFLPVLFSLVATIVVKKVYKNIDLEVNNE"
     gene            14713..16137
                     /gene="panF"
                     /locus_tag="PXD66_000284"
     CDS             14713..16137
                     /gene="panF"
                     /locus_tag="PXD66_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HDL6318619.1"
                     /translation="MNSEMILPLIAYLIFVFGVAFYAYRKRQGGSFISEYYIGGRSMS
                     GFVLAMTTAATYVGASSFIGGPGAAYKYGLGWVLLAMIQVPAVLLSLGALGKKFALLA
                     RKHNSVTINDMLLARYQNKFVVWIAGLALLLSFFAMMTVQFIGAGRLLETTLGIPYQT
                     AVMIFAITVGLYTFIGGFRAVVLTDTIQGLVMIVGTSILLAGVIYATGGVENAMHTLE
                     AINPQLTTPYGIDERPLDFTFMTSFWVLVCFGLIGLPHIAVRSMACKNSQALHRAMII
                     GTVVIAILMFGMHLSGVLGRAVVPDLKVPDQVIPTLMVQVLPPVVAGIFLAAPMAAIM
                     SSIDSMLIQSSSTLIKDLYLAIKPNAINNEKKIKLFSTITTLTFTVLLVFAALTPPEM
                     LIWLNLLSLGGLEATFLWVIVLGLYWEKANATGAIYSMLAGLSCYVLMTTFKIAIFSF
                     HAIVPSLLIGLIAFLIGNRFAPKV"
     gene            complement(16214..17404)
                     /gene="nhaA"
                     /locus_tag="PXD66_000285"
     CDS             complement(16214..17404)
                     /gene="nhaA"
                     /locus_tag="PXD66_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HDL6318620.1"
                     /translation="MEKLKEFLKSEASGGVLLLVFAFAAIVCANTSLSDFYFEFLQTI
                     VSVQFGEFALNKPLLLWVNDGLMAVFFTLVGLEVKKELFEGSLSSYKKAIFPAIAAVG
                     GMIVPAIIYWAINQNHPEYHNGWAIPMATDIAFAVGIVALLGTRVPLALKAFLLALAI
                     IDDLGAIVVIAIFYSQEISVQALTIAAIAIIGLVLLNRFKVTSLCAYLVVGLVLWTSV
                     LKSGVHATLAGVIIGFCVPLKGKHGERPAEELEHILAPWCAYMILPIFAFANAGVYLL
                     EMGMEQLSSPLPLGIALGLIIGKTLGVFGFSYLAVLFRLASLPQGVNFKQIFAIAVLC
                     GIGFTMSMFLASLAFDVSEAGESITALARLGILLGSAVSAILGYTLLRMATKQNNPEN
                     NATY"
     gene            17572..18000
                     /locus_tag="PXD66_000286"
     CDS             17572..18000
                     /locus_tag="PXD66_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDL6318621.1"
                     /translation="MTHDWLYWALASALFAALTAIFAKVGLQGVDSDFATFIRTVVII
                     IALIAFLTYSGKWQPLDSLSSKNWLFLILSGLATGLSWSAYFKALQMGNASQVAPIDK
                     FSLVLVTLFAVIFLGERPSGQDWLGIGLVAGGVLLLAFKR"
     gene            18006..18728
                     /locus_tag="PXD66_000287"
     CDS             18006..18728
                     /locus_tag="PXD66_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HDL6318622.1"
                     /translation="MQNFANIEIAFIHRKAPLPSDFSYPEMPGGLTEKQVQKWQSRRV
                     AFFLLTELFKKHHLDLDLLADMQRSPSGRTFVDSEQIDFNISHSGEWIAVIFSYSFAK
                     LAVGIDIEHPQKIRRYHDLICHYGNAEEQNVLLANHSPILSNLAERFYLSWCLREAIL
                     KSQGVGIVKLSEVKHLPLEKQIFSAHCPKGKLHFVSELPFYLSYFYQQPQNMLLSEPL
                     LYHWQNGQFQPIKCQSLVYDVN"
     gene            18738..19157
                     /gene="psiE"
                     /locus_tag="PXD66_000288"
     CDS             18738..19157
                     /gene="psiE"
                     /locus_tag="PXD66_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HDL6318623.1"
                     /translation="MPKKSLEEHHSSFGKIASETFQWIMNIALLVTGALLSYSLFAEA
                     YSLYELSFSDSEKFQIVEKIVIFFLYFEFLALIVKYFKSNYHFPLRYFLYIGITAMVR
                     LIIVDHSNALHTLLFALSILVMIIALYIVHTDRLHKS"
     gene            19166..>19762
                     /locus_tag="PXD66_000289"
     CDS             19166..>19762
                     /locus_tag="PXD66_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDL6318624.1"
                     /translation="MKLTQYQHNDLQILNRETVYKGHFELQKITFRHKLFSGEMSGEV
                     VRELLIKGEAAALIAYDPTLDKVVLIEQVRIGAYDAGQKKSPWLLELVAGMVDKGNEE
                     PAEVAMREAKEEAGLEVTTVQHALSVWDSPGGQLERLHLFLGLIDSTQVKSGEVHGLL
                     EEENEDILVHVVSREQAYQWVEDGTIDNVIAVIGLQWLQ"
BASE COUNT         5580 a         4404 c         3724 g         6054 t
ORIGIN      
        1 tcagcctatc agaacaatag gaaaaattta aattggagga atcaagtgaa tattcgagat
       61 ttagaatacc ttattgcact tgcagaacat aagcatttcc gtcgtgcagc agaatcttgt
      121 aatgttagcc aacctacttt aagcgggcaa attcgcaaat tagaaggcga actaggcact
      181 attctgcttg aaagaaccag tcgcaaagtc ctctttacgc aagcaggctt aacattagtt
      241 gaacaagcca aggtagtttt acgtgaagta aaaattctaa aagaaatggc aagcaaccaa
      301 ggtaaagaga tgtctggccc gcttcatgtt ggtgtaatcc caaccttggg gccttacatt
      361 tcgccaatta ttgtgcctgc attaaaagca gaatttgcag atttagagct gtatatttac
      421 gaattacaaa ccaaccaatt attagatcaa cttgaatccg gccagcttga ttgtggcatt
      481 atctcatttg taaaagagag tgaatctttt attgaagtgc ctatctttaa tgaagaaatg
      541 tacttggtag ttccagaaca acacgaatgg gcagcgctta aaaaattcga tatgaattta
      601 ttaaaaaacc gtgagttgct ctttttagac agtggccatt gtttacgcac taatacgcta
      661 gactattgtc tatccattgg agcaaaagaa aatccacact ttaaagctac caaccttgaa
      721 accttacgca atatggtagc agcaaatgtt ggcctagcat tgattcctaa attagccgct
      781 aaaccgatta aaggagtaca ttatattcca cttgaaaacc cgaaaccgtt tcgagctgtg
      841 ggaatggtgt atcgaccagg ttcaccatta cgcgcccgct atgagcgttt agctcaagcc
      901 attactaaaa ttatgcaatc cgaggcgtaa aaatggcaaa cggtatgagt gtgcgcgcaa
      961 ttcagaaaga aaaaacacgc agagcattag tagaagccgc ctttaatcaa ttaagctcag
     1021 aaaaaaattt ctctaatctc agtttgcgtg aagttgcacg tgaagctgga gtcgcaccga
     1081 cttcgtttta ccgccacttt aaagatatgg acgaactagg cttagcaatg gtggacgaat
     1141 ccgggctact attacgccaa ttaatgaggc aagctcgaaa acgaattgca aatggcggaa
     1201 gtgttgtcgt ggtttcagtg gaaacattct tcgaactgat tgccgaccgc cctaacgttt
     1261 tccagctatt actacgcgaa agctccggta catcgcaagc gtttagaacc gcagcatcac
     1321 gcgaaattca acattttgta gctgaattgg cggactatat tatgagcaaa gataatcaca
     1381 gtaaccgaga acttgcttat attcaatcgg aaggtttagt cacgctagta tttactgccg
     1441 gttctcatgc tttggatatg aacaatcaag aacgggaaaa actaaagcaa cgtgtcatta
     1501 tgcagctaag aatgctgatt cgtggctcca cctattatgc caataaacaa gcttcatcaa
     1561 cataaaaaaa cagacgccta agcgtctgtt tttattcttt cgtaatatat gcagtcgata
     1621 gctttaacca ttctagtgct aattgatgat gttcagataa cgcattccat aacgcatctt
     1681 ctgacaagat actataattg cctttagtat ctaactgttc atcaaaacta tcatgtaatt
     1741 cacgccattc cgggctagca taaaaccgac ttaagggttc caaaagtgca gaagctcgtt
     1801 tccattgctc aatccccttt tccaattctg gtaatagctc tacccattgg ttataacaat
     1861 cctgcatttt ctgaatatct gcttttttca ttctttaccg cttacaccat aaattttagc
     1921 ctcaatataa cataaaaatc cccttccgaa gaaggggatt tcaactcaat taggctaaat
     1981 taccactggt atccaacgcc tacaccgcca ccaaagtcgc ctcggctatt cgcattacct
     2041 tgtaatttca agataagctt accgttatca cttgaacgtg aataacctac tgcaagcgcg
     2101 ttttcacctt taaatgtacc acctgctgca gccaccatag atttacctgg aatgtaaacc
     2161 tgcggtaacg ctgctgctgc atttgaaccg gcaataccag cacgggcttc acggttatta
     2221 cgatggatct tgttatttaa gtgattaaca gcacctttta actgaccaac attaacagca
     2281 tcggtatctg cctcacctgc tttaacatta gtaatacggt taccaccatt atttaagcca
     2341 tctttcgtta agcttaccgc ttttcctgca gagccattat taatggttac gccattattg
     2401 ttcaccaatg tatcaccagt cttgctagta aattcagggg tatctgaaac ggctacagtt
     2461 acatcagaac ctttacgtgt cactttgata ttattacctg cattcacgct cactgtacta
     2521 cctgctttta ctttcgcagg ggctttatcc ccttcaaccg cacctgtacc aactgcttta
     2581 gaatctacgt tccagcttac gttgtttacg gcatttgcta ctgttgttgc attcacaaag
     2641 cgtgaaccgg taccgttagg atcttgaaca ttgattttgc cgttatcagc aacggttaaa
     2701 tctgtgatgt ttacgttcac tttcacctca ccagccttat cactggtaat agttgtacca
     2761 ttaccattta cgatattgat agaaccatct tcatcagtac caatgttatt accattcact
     2821 ttcgcatagt tgttcagctg tttaccgttg ataacatctg tgcttactgg gctaatatcg
     2881 ccatctgcta cattagtgat cttagttggt ttaccgtctt tatcaccgac tttgatgtta
     2941 tcaccatcat tagtgatttt cggaccattc tcactgaact gtacgctatt gaagctcaca
     3001 tcttttgctg tcgcaaaagt aaattgatta ccatcacgtt taacagttaa gttatcacct
     3061 gcttcaaaca caacttcttc gcccgttttc actagcttag tcgcagcttg gtcaccggca
     3121 gccttattac ctgcagattt aatattaaag cctgcattat tcactgcatc tgcaacagtt
     3181 tgagcaatca ataacgcatt tggctgagtt ggtgctttca ccgtaccatc ttcgttgtta
     3241 cctaactcac tggtatttgc agtgatgtta ccttcattat ctactttaat agtagtaccg
     3301 tcagttttcg cttcaacgtt ataagtagtt gaaccatcag cattcgtgct aggtgtgata
     3361 gttacacctc ggttaccgcc cactttcgta gtagctgcat tagttgccgc ttgtaaatca
     3421 cctaagttca ctacgttgtt tagcttgtcg ccttcagcat ttttaatcgc ttctgcacgg
     3481 tctttaccct ctggggttga gccatcttcg ttacgaacac tacttgccac attgcctaat
     3541 tgaacagggg cttcacctgt gaatttagcc gtattgcttg gcgttgagct atcagtagga
     3601 tcttggttta caaactcaat cgggctatct actttaatcg tattcttacc gtctttgttt
     3661 tcaaccgtga ccgttgtacc tttaccgtta ataaaatcaa ccgtgtcatt atgtaccaca
     3721 gtatcaaccg ctttgccgtt accttgtaaa ttccagcctg catttaatac atcacctaat
     3781 gttgctgcat tacttaactt atcagctaag ttggttggat tattgtctgc tggttgaacc
     3841 ttatcatctg cagtcacagg tatcagatta gacgcaacat tgctaatcgc tttattacct
     3901 gcattgatac catctttatt gatagtaatt ggattatttg caggatctgc ttcatcagtt
     3961 ggtacagtta cagtgttaga ggttacgctg ctaaacttaa catcatcttt agtcgcataa
     4021 atgactttac catcagcttc ttgcttcaca gttaagtttt tacctgcaac catttcaact
     4081 gctttacccg gattaatcag ttcatcacca cctgaaagct tctctccacc ttctacagcc
     4141 gacgttttca acgtaaagcc tgagttattg atggcgttgg taatatcacc tgctgtcact
     4201 aatttcttag catcatcagc ggttggttca tcaattctac cgtcattatt tgcatcattt
     4261 aatgccgtag tgttagcctt gatcgcaccg gttgtaggat cttttacaat tgttacatcg
     4321 tccgtattca cagaaagatc gaacacattc gcgccatcag cattttttgt cgtgttaacc
     4381 gtaactgatt tatcagtcga cttcacttct tcacgagatg ccatagcgaa agtataaagc
     4441 tgactaccat ttactgcatc tttactatct gcatttacgt caccggcagc tacattagtt
     4501 actttgactg cattaccgtc tttgtcacca acattgatat taccaccatt gttagtaatc
     4561 ttaggacctt catttccgaa ctgtacacta tcaaaattca cattttttgc agttgaaaca
     4621 gtaatgttat tcccatcacg ggtaatctca atgtttttac catttaagaa ctgaaccgta
     4681 tcacctgttt tcaccaggct cttctcgtca ccgtttgctt taatgttaaa acctgcattg
     4741 ttaattgcat ctgcaacggt tttagctgtt actaatgcat ttggttctgt aggctcttcc
     4801 actttaccat cagggttatt tgttaactcg ctggtgtttg ccgtgatttc gcccttatcg
     4861 tttaccttaa tggttgtacc atccgttttt gcttcaacgg tataagtggt tgagccgtca
     4921 tcgttagttt tagatgttac agtaacacct ttatcacctt ccactttggt ggttgctgct
     4981 ttcattgctg cttgtacatc acccacattc acagcattat tcaacgtatc acctgttgcg
     5041 ttgttaatat tatccgcaat cgctttacgc tctgcatctg ttggtgctgc tttatctgca
     5101 aatgtaccac ccacgccgct cttcagatta gtgattgagc ctgttgtatc tggtttacca
     5161 ttgctcatat caatgctgtt gaatgcaaca ttatctttcg ttgtgtaagt gaatttaccg
     5221 tcagcttggg taagtgtcat atttttaccc gcatcaaagg taactgtttc acttggcttg
     5281 attagctgtg ttttcttacc tttggtttca ccaccatcaa caccatcacc tgctgttaca
     5341 ttaaagcctg aattattgat tgcattagca atatcacctg ccgtcactaa tttcttagca
     5401 tcgtcacctg taggctcagt aactttacct tcaggagaat tatttaatgt tgtggtattc
     5461 gctttaactt cgcctgttgt cggatctttc acgatagtaa cgttgtcagt attcactgtc
     5521 atattaccgt cattatcacg accgatagat ttatccgttg caacatctac cgtatattca
     5581 gtattgcctt tatcatcttc ctttccggct ttaacagtta cggctttaga accagctact
     5641 acagatgagg ttttctctga attattaatt gcatcagcca cattaccacg agttactgta
     5701 ggagcacctg ctttcgctac atctaccgta tagattgttt gaccattatc acctgtttca
     5761 ggtgcaccaa cagttgctaa acctgaacct ttcacttcgg ttttgtgacg ctgctgcttg
     5821 ttttaactga cttacattca ccgcatcagt atcagcttca cctgctttca cgcctgtaat
     5881 tttggttgga ttaccatcat tacctgcaat attaacatta ccatctttat tcgtgatttt
     5941 cgggccatta tcaccaaatg ttacgctatc aaatttcaca tctttcgctg tttcgtacgt
     6001 gaatatagtg ccaacacgtt taacttttaa gttatcgcct gctgcaaaaa tcactttttc
     6061 gcccggatta atcaactctg ctgcttcttg atcaccatca gctttattgc caccagattt
     6121 tgcattccaa cctgcttttt gaatcgcttc agaaactgtt tttgtagttg caactttatc
     6181 ttcttctgca ggtgtagtta ctgcacctgt tgtgttatca accgtgatag aaccggtatc
     6241 cacatcaaag gttactttac gccatcagct tcttgtgtaa catttgaaac tgtaccttta
     6301 ccgtttacaa attgtacaga ttcatttggg gtcactgtgc ctttggtttc acccgcatta
     6361 tctttaatat tgaaaccttg gtttacatag ttatttaagt tcgtaattgc atcgccaacg
     6421 tttgttgcag tattaccata ctgagttcca gctgcattcg ttacattata tttaggctga
     6481 gtcacagagc catccggatt cacaacagaa ccgccaccaa aattatttgc agttgaattt
     6541 gcaaggttac tggttgcatt cattgccata taaagttgtg aaccattaat cgcatcggtt
     6601 gatgtagcag aaatctgacc cgctgccaca ttttggattt gacgctcttt acctgcagag
     6661 cctacagaaa caacatagtt agtatcttta tttaccccgg caaagccatt ataagtaata
     6721 ccccctaccg ttgcattttc agttggtgtt gctgccctta cgactgattc gctacccaaa
     6781 gcaacagaac tattcagagt agcttgagct cccataccta atgctgtagc aaattgagct
     6841 gttgcattag cacgtgtacc aacagccact gctgattgtg ctgaggcaat tgaatttgca
     6901 cctacggcta aagaggcatt tgcactcgca tttgctgcaa caccaaaggc agaagctttt
     6961 tcagccgttg cattagcatt agaacctacc gctgtagcat cacctgctgt cgcatttgtt
     7021 tggcgaccaa gtgcggttgt accattagcc gttgcatttg ctgaatgacc aactgccgta
     7081 gcttgaatac cttttgaaac cgtagcaaca cctaaagata acgctccgtt accatttgca
     7141 tttgagtttg tgcctaatgc tactgccgct tgctctccac ctgttgccgt tgcattcggg
     7201 ccgatcgcaa ttgagtttct gctcttcgcg ccatcattag cttgattctc aggtacggtt
     7261 gaattcacag agaaatattg aatttggctt gcttgcgcag gaatattgac agaaagatca
     7321 taaataaccg tgccatttgc atttctctct gtacttttta cagttactgt accatcgttt
     7381 gaaacaacag tagcttgggt atctttcgta aagtcatcaa tggctttctt agttaaacca
     7441 atttcatagt tagttgtatt cgcagcagtt gcagctttac ctgttacagt aatgtaatca
     7501 gggctagaag aatttgccgt ggtgttatac gcatttactg tataaatagt ttgcccattt
     7561 gcaccggttg catttgtaat attaacattg ctacctgcaa caacttctgt ttttaccgct
     7621 tttaatgctt cttcaatcgt atttttattt gttccaccaa tattagtata ggtaacagaa
     7681 ccatcaggct ttactgtagc attaccacct aaaatatttt tagttgaatt agcaaggtta
     7741 cctgttgcgt tcattgccat ataaagctgt gaactattaa ttgcatcggt tgatgtagca
     7801 gaaatctgac ccgctgccac attttggatt tggcgtttga tcgtgtttga gccaacagaa
     7861 acaacattcc ctgctgccgt agtattagcc gcaaagccgg aataagtaat accatttacc
     7921 gtcgcagaag gaactgccac agcgttggaa acggttgaat tactccctaa agcaaccgaa
     7981 ttatttagcg ctacattagc acctgagcct aaggcaatag caaaaccggc acttgcttta
     8041 gcactatcac caattgcaac cgtagattgg ttggaagata ctgcacttac ccctaaagct
     8101 gtcgcattaa cacctgaaac tgtcgcatta taaccaatcg ctgtacctct agttgcagta
     8161 gaagttgcat tagcattagc acttgaacct aaagctaaag aataagtagc atttgcaaca
     8221 gacttatccc ccattgcacc tgaacccatt gcaaatgaac gagtgccact agcggcagat
     8281 ctccatccca ttactgttga acggttacca gtagctagag ctcctgcacc aaaagctgtt
     8341 gcacggttac ctgttgcagc agtatccgta cctaatgcaa tagcatttgc tgaagtcgct
     8401 ctagccccca taccaatagc aatagaagga actaatgccc aattctgacc agtgttattg
     8461 agactctcta caccaatatt atagtaatgt aagaagtatc ttctggatcc actaaatttg
     8521 ttctatgcgt tgcattcgta tctgtaccaa ttgcaatagt agatctgcca gttgagattg
     8581 aatccgcacc aatagcaatt gcgttctccg atgatgcagt tgcttgtgtt gaaatataat
     8641 tacttgttgt tgaacctata agatacaaca gccacagcat cattagctcc caacacggca
     8701 ctagctccag ccaataaagc caatgttgcc ttacttgcag aactaacaga tttcgtttta
     8761 cctttcgctc gttgtaattc agatactgca acccaacttt gtgttgcctg attccaaata
     8821 actttaaaaa tttgattcat aatcgaccct atataaaaat aaaaaagatt acgtagaacc
     8881 tatgttcaac ataatcttca ccaatataaa aaaccgccac ctatttaagt gaaataactt
     8941 acttatagac tcagaaattt aagtgcgcgt attttacgct atttgtaata taattcatct
     9001 tctggagtgt aaatttattg taaataatac gaggaatcta accgcttgtt ataacgcaaa
     9061 acaccttgac ttaaatcaag ccactacctc attttgaggg gagatttata cgcagtgaaa
     9121 ttttaaaata agctaaggtt tacggataat cgttcaacac tattgaatta tcactgtagt
     9181 tatcgttaaa aaggtatgct tttatgaacc aattcttccc tctaatcttt agcttaactc
     9241 tattttccgg cgtaactttg gcactagaaa aagcacctcg aactatgccg acagaaggaa
     9301 taaataagcc cctacttgga caaattctat tctttgacaa tatgctgtca cacaacggtt
     9361 cacaaaactg ctccagctgc cataatccgg attatgcctt tattgataac cgcactactc
     9421 cggcaaaagg ggtggtttca caaggagata ctccaaccaa attcggcaaa cgcaactccc
     9481 cgaccatgct ttatgcgaaa tactcgcctg agttccactt tgatgagcaa agtcaagaat
     9541 atatcggcgg gcagttctgg gacggacgag ccaaaaatct cgccgagcaa gccggtatgc
     9601 cgcctatcga cccaaatgaa atgggaatgc ctaacaaatt agaagtagcc aaaagattat
     9661 ataatgtgcc gatgtatcag cgatttttag ggcaatttta cggtgaaaag gtatgggaaa
     9721 gttatgaaag cgtttatgcg gcaatggaag atgcgattgc gacattccaa caagaaaaca
     9781 aacttctcgc tccatttgat tcaaaatatg atctattttt aaaaggcgag gtacaattaa
     9841 ctgcattgga agaaaaaggc agagctttat ttttcaataa agctcaaact aattgtagta
     9901 gttgccacca attacagagt gctgcaaacc accaagaaga aacctttagc aactatcact
     9961 attacaatgt aggcgtaccc ggtaacccag acttgatagc ggttaacggc ttcgcaccag
    10021 acttcgtcga tttaggctta tttgaaaacc caaatgttaa aggtgatgaa acacaaaaag
    10081 gcaaattcaa aaccccaaca ttgcgtaatg ttgccgtgac tgcactttat atgcacaacg
    10141 gtgtgtttaa agaattaaag actgttttgc tgtttttaga cagctacaat aacccggagc
    10201 gaaaaatcaa ccctgaaacc ggcacggcgt ggcaacaagc agaatttgaa cccactatcg
    10261 cccaccaaaa actcaaagcc aaacccttaa gcgatgaaga aattgatgcc ctcgaagcat
    10321 ttttaaaaac cttgacggat gagcggtata gaaaataagg ctacaagcgg taaaattttg
    10381 gggaaatttt accgcttgcg tttaactacc gtgttagctc actttctaac ttttccaatg
    10441 cctgcaaatc ttcccaatct gcctctttct gttttaaaca tttacgctga atatcaaatt
    10501 tttcgtattg ctctctgaca aagctttcca tttgtttatg cgtgattgtt ccaccatttc
    10561 ctaatacaga aaagccgtta aactcaatca acatatctac atttcttcgc cagaaaccta
    10621 acgtaagatc ttggttgttc ttcacacgta attcggcact ttcaagaaag atcattacca
    10681 agcgatttaa agaatccagc tcattattat ctaaatagtt tttcgcaatc acaatatcgc
    10741 ctttacgcac gacttttcct ttccagctag ttaatcccat attcatctta gtcgcatcag
    10801 cacgttgaca aatcaactca gcggcagtgt gtttggttat tgcataaatg agcttatttt
    10861 gcgtttctgc aaaaaacatt tgcgtatttt tatctgtttt gtcgtaatcg ctagagagtt
    10921 tgaaaagctc tcgtactttt tgatagaaac gcaattcact tgcccgaatc tcacgaatct
    10981 gttcaagtaa ttcatcgaaa taatctaaac gaccctgcgg atttttcaaa cgctcgctat
    11041 caattaaaaa acctttttcg agataagtgc gtaatgtgct gtttgcccat tttctaaact
    11101 gtactgctct tgggctacga actcgaaacc ctatcgccag aatcatttca agtgaataat
    11161 gttttacttg ataattcttg ccatcttgag cagttatcaa gtaatccttg ataactgaat
    11221 tctcatctaa ctctttgtct tttaagatat ttttaatgtg ttcggatata ttttgcacag
    11281 aggtgtcaaa aagttctgca agttgatttt gtgtcagcca agcttcattt tcaaccacta
    11341 acaaggctac attgcttttg ccgtcttccg tattataaat aattaaatcg ttcatatttt
    11401 attctgttta ttggtagaga tattagtcgc tattttcaat aaaacatgta taaatatcca
    11461 gaattgattt tgaatttttc gatgcagatc acaaaaatag agagttatac aaacaagcgg
    11521 taaaattttg taaaaaattt accgcttgta aaggttactt atcagctaat ttggcaagta
    11581 gtttttgatg aataccgcca aaggctccgt tgctcatcac taagatgtga tcggtgggtt
    11641 tggcttcttt gacgactaga tcgactaaat catcaatatt tgccgaccaa tacgccggtt
    11701 gcgataacac attggtaatc tcgctcacac tccacgcaat aatatcaggc tggtaaacaa
    11761 acactgcttc ggcatcgtgt aggcttgggg ctatttcatc tttatggaca cccattttca
    11821 ttgtatttga acgaggctct aacactgcca aaatgcgttg atttttgccg actttaccac
    11881 gcagagcatc aatagtcgct tctatcgcag ttgggtggtg agcgaaatca tcataaacgg
    11941 taatgccgtt tacctcgcct ttcacttcca aacggcggtt agcattcaca aacttgccta
    12001 atgcctcgca agccccttgc acttcaaccc ctgcgtgatg agcggcggca atcgccatca
    12061 gagcattatg catattgtgc ttgccgataa tcgaccaatg cacctcgcct tgtttaacgc
    12121 ctaagtgaaa cacctcaaac tgtgagcaat cggcatttaa cggtttagca aaccattcgt
    12181 tctgttcgcc aataaattga agctggctgt aactgcccat ttttagtgta tcttgcacgt
    12241 tttgatcagc gattgccgac aaaatacagc cactttgtgg catcgtgcga acaaggtggt
    12301 ggaactggcg ttgaatcgct tttaaatcat caaaaatatc ggcgtggtca aattccatat
    12361 tattgataat cagcgttttc ggggtgtaat gcacaaattt agaacgctta tcaaagaacg
    12421 cagaatcgta ctcgtccgcc tcaatcacga agaatggact gttaccggca cgagccgaaa
    12481 tgccgaaatt accggcaaca ccgccaatta aaaagccggt atcaatccca ttttggtcta
    12541 aaatccacgc taacattcct gttgtggtag ttttgccgtg cgtgccggaa accgccagca
    12601 cccatttgtc tttaagcaaa tgatcgtgca accattgcgg gccggaggtg taattgagtt
    12661 ggttgtccaa cacatattcc acgcacggat taccacggct catcgcatta ccaatcacca
    12721 ctaaatcagg agccggttgc agttgagcaa catcgtaatt cgggataatt tcaataccgt
    12781 gagcagctaa aaaggtactc atcggtgggt aaacattagt gtctgagccg gtcactttat
    12841 agccaagctc acgggcaatc attgccacac cgcccataaa ggtgccgcaa atgcctaaaa
    12901 tatggatatg ttttttcatc tttttatatt taaaatgtta gaatcatccc ctctcccctt
    12961 gtgggagagg gatagatttt ttcttcgtgc agaagaaaaa atcagggaga ggggtaagcg
    13021 gtcattttta ggcttaatat tgtaagctcc ctctctctga aaaatatttc tgaacgaaat
    13081 atttttcggt ctcaatactc gctacgctcg ctcctgtgga gccgttggca aagccaacgt
    13141 tcaaatgtaa acatttgtcc cacaagggga gaggtgcgct ttagatgagg cgtaccatcc
    13201 ctcgttcaca tcaattaatc aatattcttc aactcttctt ccgaaagctg ctcttgttgg
    13261 ctttgctcaa cattgccatc tttcacttta aattcaaggt tttggaaata agcttcacgg
    13321 aaggtaacat aagggtcttg agcttgctct aacaacgcat cttgttctaa taatttagaa
    13381 cgggtatcta ccccttgtac cacaaattta gcgatagacc aaggtgaccc aacataagtc
    13441 agcaccgaat aaccggtttc caccgcacta ccaatacctt gacgaggtgt ggttgctcca
    13501 taagccggca tcataatata ggagccggaa ggcacgccgt gagcgcctaa actctcacct
    13561 aaaccacgtt gtccgttatt taaacgtaac ccctcatcta aactcgccca gtcaattaac
    13621 ccacctaaac caaacacagt gttgatccag aagcggttga agtgaaccat cgctttttta
    13681 ccttctcctt caattaagcg gtttacaaag ctcaccggct catcaaggtt attagcaaca
    13741 ttaattaagc ctgttttaac cgggcttggt acatactctt tccaaccttt agctaccggt
    13801 tttaacacat aagggtctaa atagtcgtaa ttgactttcc acatcgcacg gttaaacccc
    13861 tcaagtgggt cgtctcgcac gccggtttct gggttaatgc ttgatgaaca agcggttaaa
    13921 aatgccacac tgagtagcat aaacatcggt ttgaacgact tgagtttcat aaattgaacc
    13981 ttttgcagag aaatttgtcc attgtagcga agttgataaa aaatacaaaa caaaaagccg
    14041 ttttgcctct agaaatctag acgtctagat tgagatatag ttcgcttttg attacaatac
    14101 aggcaacatt taaggagcaa acaaatggca gactggaacg gcgaatatat tagcccttat
    14161 gccgaacacg gcaaaaaaag tgaacaagtc aaaaaagtga cggtatccat tccaattaaa
    14221 gtattggaaa ttttaactaa tgagcgaacc cgtcgccaaa tcaaaaatct tcgtcacgca
    14281 accaacagcg aattattgtg cgaggctttt ttgcacgcct ttaccggtca gcctttgccg
    14341 aatgatgaag acttgatgaa agacagggca gatgaaatcc ctgaagcggc aaaacaaaaa
    14401 atgtgtgagc tgggaataga cccggacgaa tggcaatatt agacacacct tgcaactgcg
    14461 aggttctttt tatgttttaa ggagacttta tgtatcaaca attaacaaca gaagcacgtt
    14521 gggctgtgtt gcttacgctg atttatctgc tcggctgggt aggttttgct tacttttcac
    14581 cgcaaggcag aggcattttc ggctttccga tttggtttga actttcttgc attttcttac
    14641 ccgtactatt tagcttagtt gccaccattg tcgtcaaaaa agtgtataaa aatatcgatc
    14701 ttgaggtgaa taatgaatag tgaaatgatt ttacccctga ttgcttattt aatttttgtg
    14761 tttggcgtag cgttttatgc ttaccgtaaa cgccaaggcg gaagctttat ttcggaatac
    14821 tatatcggtg ggcgttcaat gtcgggcttt gtgttagcaa tgacaaccgc cgccacttat
    14881 gtgggggcaa gttcctttat cggtgggccg ggtgctgcct ataaatacgg tttaggctgg
    14941 gtgttactgg caatgattca agtgcccgca gtgctactct ctcttggagc attaggcaaa
    15001 aaatttgcct tacttgcccg caagcataat agtgtcacca ttaacgatat gttattagcc
    15061 cgttaccaaa acaaatttgt ggtttggatt gcaggactag ccttattact ttcgtttttt
    15121 gcgatgatga cggtgcaatt tatcggtgcc ggtcgcttac ttgaaaccac actcggaatc
    15181 ccttaccaaa cagcggtaat gatttttgcc atcacagtcg ggctttatac ctttatcggc
    15241 ggttttagag cggtggtgct aaccgatact attcaaggtt tagtaatgat agtcggcaca
    15301 agcattttac tcgctggtgt gatttatgcc acaggcggag tggaaaatgc aatgcacacc
    15361 cttgaagcca ttaacccaca acttaccacc ccttacggca ttgacgaacg cccgcttgat
    15421 ttcaccttta tgacttcatt ttgggtattg gtttgctttg gtttaatcgg cttaccgcat
    15481 attgcggtca gaagtatggc atgcaaaaac agccaagcct tgcaccgagc aatgattatc
    15541 ggcacggtag tcattgcaat tctaatgttt ggaatgcacc tttcaggcgt attaggcaga
    15601 gcggttgtgc ctgatctcaa agtgccggat caagtcattc caacactaat ggtacaagtg
    15661 ctaccaccgg tggttgccgg tattttcctt gccgccccaa tggcagcgat tatgtcttcg
    15721 attgactcaa tgttgattca gtcgtcttca accttaatta aagatctcta tctggcaatt
    15781 aagccgaatg ccattaataa cgagaaaaaa atcaaattgt tctccactat tactactctt
    15841 acttttacgg tattactcgt gtttgccgcc ctaaccccgc ctgagatgtt aatttggctg
    15901 aacctactct ctttaggtgg tttagaagcg actttcctat gggtaattgt actagggttg
    15961 tattgggaaa aggcaaatgc caccggtgct atttactcaa tgctggcagg cttaagttgt
    16021 tatgtattaa tgactacctt taagattgct atttttagct tccacgccat tgtgccttca
    16081 ctgctaattg gtttgattgc tttcttaatc ggcaaccgat ttgctcctaa ggtgtaaaat
    16141 aaaataggca tagtggtatg gaagttcact atgcctattt tgcaaaattt ccgctcaatt
    16201 caaccgcttg tagttaataa gtggcattat tttctggatt attctgctta gtcgccattc
    16261 taagtaacgt ataacctaaa atcgctgaaa ccgcagagcc gagtaaaata cctaatcgag
    16321 ctagagcggt aatactttcc ccagcctcac ttacatcaaa ggcaagactt gctaagaaca
    16381 tcgacattgt aaaaccgata ccgcatagca ccgcaatggc aaaaatttgc ttaaaattaa
    16441 ccccttgcgg caggctggca aggcggaata atactgctaa ataactaaaa ccgaatacac
    16501 ctaaggtttt gccgataatt aagccgagcg caatacctaa cggcaatggt gatgagagtt
    16561 gctccattcc catttctaat aagtaaacgc cggcattagc gaaggcgaag atcggtaaaa
    16621 tcatataagc acaccaaggg gcaagaatat gttctagctc ctctgccggt cgttccccat
    16681 gtttaccttt cagcggtacg cagaagccga tgatcacgcc ggcaagggta gcgtgaaccc
    16741 ctgattttaa aacggaagtc catagcacta agcctactac caaataagca cagagtgagg
    16801 tcactttaaa gcggttaagt agcactaagc cgataatcgc aatcgcggca atagttaagg
    16861 cttgcacact gatttcttgc gaatagaaaa tggcgattac cacaatcgct cctagatcat
    16921 caataatcgc taatgccaac aaaaatgcct ttaaagccaa tggcacacga gtgcctaata
    16981 acgccacaat gcccaccgca aaggcaatat cagttgccat tggaattgcc caaccattat
    17041 gatattcagg gtggttttga ttaatcgccc aataaataat cgctggaaca atcataccac
    17101 ctactgctgc tatcgcagga aaaatagctt tcttataact tgaaagcgaa ccttcaaaca
    17161 actctttttt tacttccagc cccaccaacg taaaaaacac tgccattaag ccatcgttaa
    17221 cccagagtaa tagcggttta ttcaaggcaa attcaccaaa ctgtacacta acaatcgttt
    17281 gtaaaaactc aaaatagaaa tcacttaagg aggtgttggc gcacacaatt gctgcaaaag
    17341 caaaaaccag aagcaatacg cctcctgagg cttcagattt taaaaattct tttaattttt
    17401 ccacttaact ttctcctatt aatatttgtt tattgtattt tcatttctat acattccatc
    17461 ttttaccaaa aattttgcga agatcaaata gaaaatttga taaactagaa ctaactcgca
    17521 ttttattgct ccatatgatt agaaattaac ctaaaatatt aaaattttgt tatgacacac
    17581 gattggctct attgggcatt agcctctgca ctttttgcag cattaaccgc catttttgcc
    17641 aaagttggct tacaaggcgt agattctgat ttcgccactt ttattcgcac ggtagttatt
    17701 attattgcac ttattgcctt tctcacttac agcggaaaat ggcagccgtt agatagcttg
    17761 agcagcaaaa actggctgtt tttaatctta tccggcttgg caacaggatt gtcttggtcg
    17821 gcgtatttca aagcattgca aatgggtaat gcttcccaag tcgctccaat cgataaattc
    17881 agcttggtgt tagttaccct ttttgcagta attttcttag gcgaacgccc aagcggacaa
    17941 gattggcttg gcattggctt agttgctggt ggcgtactgt tacttgcctt caagcggtga
    18001 tttttatgca aaattttgca aatattgaaa ttgcgtttat tcatcggaaa gcacctctgc
    18061 cgagtgattt ctcctaccct gaaatgcccg gcggtttaac tgaaaaacag gtgcaaaaat
    18121 ggcaaagccg ccgagtcgcc ttttttctgc tgacggaatt gtttaaaaaa caccatctgg
    18181 atttagattt attagctgat atgcaacgct ccccaagcgg tcgcactttt gtcgattctg
    18241 agcaaattga ctttaacatc agccattcag gggaatggat agcggtcatt ttttcgtatt
    18301 cttttgcaaa attagcggtc ggcattgata ttgaacaccc acaaaaaatc agacgttacc
    18361 acgatttaat ctgccattac ggaaacgctg aagaacaaaa tgttttatta gccaaccact
    18421 cccccattct aagcaacctc gccgaacgct tttatttgag ctggtgcttg cgagaggcga
    18481 tattgaaatc gcaaggggtg gggattgtga aactgtccga agtaaaacat ttaccgttag
    18541 aaaaacagat cttttccgcc cattgtccga aaggcaagct gcattttgtg agcgaactgc
    18601 cgttctattt aagctacttc tatcaacaac ctcaaaatat gctattatct gaacctttgc
    18661 tataccattg gcaaaacggg cagtttcagc ctataaagtg ccaatcactt gtttatgatg
    18721 ttaattaagg ataacgaatg cctaaaaaat ctttagaaga acaccattcc agttttggca
    18781 aaattgcttc cgaaaccttt cagtggatta tgaatattgc cttgctagta acaggagcat
    18841 tgctttctta ctcgctgttt gctgaagcct attcactcta tgaactttcc tttagcgaca
    18901 gtgagaaatt ccaaattgta gaaaaaatag taattttctt tctctatttt gagtttcttg
    18961 cgttgattgt gaaatatttc aaatcgaatt atcactttcc gttacgctat ttcctctata
    19021 tcgggattac tgccatggtg cgtttgatta ttgtcgatca ttctaatgcg ttgcatacac
    19081 tattattcgc actctctatt ttggtgatga ttatagcatt atatattgta cacaccgatc
    19141 gtttacataa aagctaggaa ctaatatgaa gctaacccaa tatcagcaca atgatttaca
    19201 gattctaaac cgagaaaccg tctataaagg gcattttgag ctacaaaaaa tcactttccg
    19261 ccataagctg ttttccggtg aaatgagcgg tgaagtggtg cgtgaactct taattaaagg
    19321 cgaagcggcg gctttaattg cctatgatcc aaccttagat aaggtagtat taattgagca
    19381 agtgcggatt ggagcttacg atgcaggaca gaaaaaatcc ccttggttgc tggaattggt
    19441 tgccggtatg gtagataaag gcaacgaaga gccggcagaa gtggcaatgc gtgaagccaa
    19501 agaagaagcc ggcttggaag tcacaacagt gcaacacgcc ttaagcgtat gggacagtcc
    19561 cggtggacaa ctggaacgct tgcacctgtt tctcggcttg attgacagca cccaagtgaa
    19621 atccggcgag gtacacggct tactggaaga agaaaatgaa gacattttgg ttcacgttgt
    19681 aagccgtgaa caggcatatc aatgggtaga agacggcaca attgacaatg tgattgcggt
    19741 aattgggcta caatggttgc aa
//
LOCUS       DANLXF010000013        19713 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.013, whole genome shotgun sequence.
ACCESSION   DANLXF010000013 DANLXF010000000
VERSION     DANLXF010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 19713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19713
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.013"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            46..342
                     /locus_tag="PXD66_000290"
     CDS             46..342
                     /locus_tag="PXD66_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HDL6318625.1"
                     /translation="MTAITIPQFEEVTSKQFQSEFGRIVDIVKGKTPVAITQYGRPTM
                     VLFPYDQAVEARRLLAKQRLLEMLAERDKNPPAALAEITLEELDKMIDEERNAL"
     gene            332..748
                     /locus_tag="PXD66_000291"
     CDS             332..748
                     /locus_tag="PXD66_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HDL6318626.1"
                     /translation="MHSDYLVIDTNILISGLLFPKSNPNLAVKKALLGYTICVSQATF
                     DEFLEVSARPKFAKYFSIQEREEFIANLLDAVKFVEIKETITDCQDPKDNKFLEVAIS
                     AKAKYLVTGDKKDLLSMNPYRNVEIISVSEFLAETR"
     gene            748..2406
                     /locus_tag="PXD66_000292"
     CDS             748..2406
                     /locus_tag="PXD66_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HDL6318627.1"
                     /translation="MKRFLTLFLLLFSSFSLAETHQIGILAQRGEAYTRTHWQPWVDW
                     LNSQFPNERFELVPLGINEASRRAELDFLLTNQAQFFYLSNQNVRWLATLNSPFSANG
                     EQGAVGSSIWVRADSHYQQLSDLKNQTISAVDNDAFGGFLLGLYQFHQAGMRQNRDFS
                     VQFSGFPVENALALLAEKQVEAAIVPVCLLEELEKEGKFKRSDFRLILQNPQAQGCLA
                     STPLLPNWSLAAMENVPNELAVQLATRLLNSHNPDLPRWTLPFSSAQADHILRELYRH
                     PQQKSLWATVLDWVRLNKFGLLAVVLFILLNLVALRYQVYRKSKALQQAHCKMQQYQQ
                     ELTRADRLALLGEMTTGFAHELKQPLSAVRMYAEGLKSQNSDPYQQRILDKLIAQVDR
                     AVKTMQSIRDWAQNRPSGEPQAVILNQLITNVIEFVAVENRQNAQISLIADRTFRLNL
                     HATVLEQVLTNCLLNALQAGASEISVRLQAVENGLEIAIEDNGGGFSPAQLEFPFVPF
                     RTDKPHGLGLGLVLCQRLMQSLNGRIVLANGENGAKVVLLLPQI"
     gene            2492..3139
                     /locus_tag="PXD66_000293"
     CDS             2492..3139
                     /locus_tag="PXD66_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL6318628.1"
                     /translation="MIIHILDDEESILDAMSFLLAPLGVEIQTWQSSLDFLAQADLHR
                     QGVLLLDIRMPLPDGQQVHQQLREAQSTLAVVIMTAHGDVPMAVAELKKGAVDFLQKP
                     ASFEQLKQAIAQAEKVSEKAVKIREISQNYAKLTEKERNLVPLIMQGFTNKQIADKLA
                     ISVRTVEVHRANVMEKMQAESLAGLVQMIGELQYKGSGNCSATFSFDFCRINLLL"
     gene            complement(3123..4061)
                     /gene="ttcA"
                     /locus_tag="PXD66_000294"
     CDS             complement(3123..4061)
                     /gene="ttcA"
                     /locus_tag="PXD66_000294"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDL6318629.1"
                     /translation="MTDDIQKSDKKITYNFNKLQKRLRRNVGNAIADFGMIEEGDRVM
                     VCLSGGKDSYTLLDILLNLQQSAPVNFSVVAVNLDQKQPGFPEEVLPRYLESIGVEYK
                     IVEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDMLATLF
                     LNMFYGGKMKSMPPKLISDDGKQIVIRPLAYCKEKDIIKYAEAKQFPIIPCNLCGSQP
                     NLQRQVVKEMLNTWDRQYPGRLETMFSALQDIVPSHLCDPKLFDFKGIKHGQTIEGIE
                     GDTAFDDVKIEAKTFLYEDDDNNFAEAGMIQFKEVN"
     gene            complement(4124..4837)
                     /gene="ubiG"
                     /locus_tag="PXD66_000295"
     CDS             complement(4124..4837)
                     /gene="ubiG"
                     /locus_tag="PXD66_000295"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HDL6318630.1"
                     /translation="MQNVDQQEITKFENMAKTWWDPNGSFKPIHLLNPLRLAYILEKS
                     NGLFGKKVLDVGCGGGILSEAMAKQGAIVTGIDMTSQPLEVAKQHAKESGLEIDYQQT
                     TIENFLAKMTACNQEKFDVITCMEMLEHVPDPLSIIQSCKALLKPDGVLFFSTINRTF
                     KAYMLVIIGAEYVLKMLPKGTHEFEKFIKPAELLAWCDQADLRCQEMRGYHFNPLTEK
                     FWLNNDVSCNYIATLKPNN"
     gene            4902..5282
                     /locus_tag="PXD66_000296"
     CDS             4902..5282
                     /locus_tag="PXD66_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HDL6318631.1"
                     /translation="MTRINLVPPTELCDQHLLAEHRELTRIPNAVAKGKFSLKGQPED
                     YKLGEGHVRFFFNKLTFLKKRYDALHEECLARGFKVQYFWAEHLPQDPSLWLDYQPTE
                     TALTVNRERIALRMPANPRFSERK"
     gene            5379..8075
                     /gene="gyrA"
                     /locus_tag="PXD66_000297"
     CDS             5379..8075
                     /gene="gyrA"
                     /locus_tag="PXD66_000297"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HDL6318632.1"
                     /translation="MSELAKDIIPVSIEDELKTSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLFSMDQNSNTYNKPHVKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDAPAAMRYTEVRMQKITQELLTDLDKETVDFSPNYDGKEMIPDVLP
                     TKIPALLVNGSSGIAVGMATNIPPHNLGEVLDGCLAYIENEQISVEELMRYIPGPDFP
                     TGAIINGRKGIEEAYRTGRGKVYVRAKASVETTDKGREQIVVTELPYQVNKAKLIEKI
                     AELVKEKKIEGISKIDDYSDKKGISIVIEVKRDAVGEVVLNHLYSLTQMQVTFGINMV
                     ALDNGQPKVLNLRQIIEAFVKHRREVVTRRTIFELRKARERAHILEGLAIALANIDPV
                     IELIRASKTAEEAREGLLARPWALGNVAPMLEAAGVGASRPEDLPEHLGVRDGEYYLS
                     EAQARAILELRLHRLTGLEHEKIVEEYKELLTEIGELIHILTSPERLMEVIREELERV
                     KTDFNDERRTEITAASGDINIEDLIAQEDVVVTLSHAGYVKYQPLSDYEAQRRGGKGK
                     SATKMKEDDFIEKLLVANTHDTILCFSSRGRLYQLKVYQLPQASRGARGTPIVNILPL
                     VKEENERITAILPIPNGEFSADKFIFMATASGVVKKVSLDAFSNVRASGLIALKLREG
                     DELIGVDITDGNSEIMLFSAQGRVVRFAETAVRAMGRTATGVRGIKLSTTEISSEEIE
                     EAEIIETDNEEAEDSSSVSLDKDRVVSLVIPRTEGDILTVTQNGYGKRTVISEYPVKS
                     RATKGVVSIKVNERNGKVVAAVQVEETDQIMLITDAGTLVRTRVAEVSLVGRNTQGVR
                     IIRTAEDEQVVSLERVCEPEDDENESLDALENAETAETSTEE"
     gene            8170..10134
                     /locus_tag="PXD66_000298"
     CDS             8170..10134
                     /locus_tag="PXD66_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL6318633.1"
                     /translation="MALINLSNAYLGFGDHPLLDHTELHIEPGERVCLVGRNGAGKST
                     LMKILAGEVQLDDGKLVFEKDIVVTRLEQDPPRHIEETVFDYVAEGIAHLADLLKQYH
                     HISLQMQTDYSDELLAKLSAVQSQLEHANGWQFENRIQDTLKLLELAPDKRLCELSGG
                     WVRRAALARALVADPDVLLLDEPTNHLDIEAIAWLENLLLDFKGSIIFISHDRAFIRK
                     MATRIVDLDRGKLVSYPSNYDKYLEEKAEDLRVEALQNELFDKKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKALREERRNRREVQGTAKIQIDNTARSGKIVFEVENAGYEIGGRTL
                     LKNFSTTILRGDKIALIGANGIGKTTLIKLLLGEIQPTSGSVRCGTKLEVAYFDQYRV
                     DLDPEKTVMDNVADGKQDVEVNGVKRHVLGYLQDFLFPPKRAMTPVKALSGGERNCLL
                     LAKLLLKPNNLLILDEPTNDLDVETLELLEEMLTDYQGTLIIVSHDRQFVDNTVTECY
                     FFEGEGVVNKYVGGYFDAKQQQENYFAATADKRLNSSKNLQKIEEKAPLAEQEKQKAD
                     QEKTKAEPTKKTKLSYKEQRELDELPAKMEQLEADIEALQAEVNSAEFFAKDPSYTNA
                     QLQKLADTEAALEQAFERWEALENIKNGNI"
     gene            10216..10974
                     /gene="tcdA"
                     /locus_tag="PXD66_000299"
     CDS             10216..10974
                     /gene="tcdA"
                     /locus_tag="PXD66_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HDL6318634.1"
                     /translation="MTERLDNYEQRFGGIGRLYTPEGLEKLRRSHICVIGIGGVGSWA
                     VEALARSGIGKITMIDMDDICVTNINRQIHAMSGTVAQLKTEAMKERIERINPECVVE
                     IIDDFLTQDNLADYLNRGYDYVIDAIDSLRVKAAMIAFCKRNKIKIITTGGAGGQTDP
                     TKIQITDLSKTVQDPLAARVRSLLRKEYGFSNKNKFGVECVSSTQQLIFPQMGEACEV
                     SATMNCANGFGAVTMVTATFGFFAVSRVIDKLLK"
     gene            11021..12388
                     /locus_tag="PXD66_000300"
     CDS             11021..12388
                     /locus_tag="PXD66_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HDL6318635.1"
                     /translation="MAIHRVNPKGSMEQLSHLEMELLAKNTQGNLHQLYRNCSLAVLN
                     SGVHTDDSRALLSQYPDFEIRLLTREKGVSLELHNPPETAFVDGKMILNIQYHLFAVL
                     RDIVFVNALKNAIRPLEETSLEALTTNTVFSILRNAKAIEMNTDPNLVVCWGGHSINE
                     TEYQYCRAVGQEFGLRELNIVTGCGAGVMEAPMKGAAIGHANQRYKNGRFIGITEPSI
                     IASEPPNPIVNELVIMPDIEKRLEAFVRLGHAIVVFPGGPGTLEELLYIIGIKLNPAN
                     HAQQLPLILTAPKESADYFYAIDQFIGETLGEEARSLYEIIIDDSVLVARKVKQAMIE
                     VKSSRYESGDAYNFNWSLKIEEDFITPFIPTHENMANLNLSLAQPKEQLAANLRRAFS
                     GIVAGNIKPETSDLIEKYGVFKLHGDRKLMEHIDKLLERFVVQHRMKIPTGEAYQPCY
                     EIVKG"
     gene            12408..13955
                     /locus_tag="PXD66_000301"
     CDS             12408..13955
                     /locus_tag="PXD66_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HDL6318636.1"
                     /translation="MYLKHIEISGFRGINHLSLQLRPNMVLIGENAWGKSSLIDALSL
                     IFNLEQALYQFQFKDFHIQYTDTPNQVRDIQLVFTFCEEHQHDLAVQFPAYLAQAAFY
                     IDNHRCVSLAVTGKLHGEQISTDYQFVDQSLKPIQIEQAENAIRQFIYHHPVYRLRDA
                     RLNNRVHNELLLKGSSQLEDDFQRELNALSSLLKYYFLTSQSRYLIKTSMQDTALWWE
                     KVKSLCLRLKQQPELVESAQQHISTLFLFNPRSTVIEKPIILFEDLNAQLHPRMIAIF
                     WELLGFLPSQRITTTNSIELLSQVPLREICRLVRHHDHTQAYWLERHSLGKEDLRKLT
                     FHIHHNRGLALFAKAWILVEGETEVWILQELARLLDIHLEMEGIKIVEFAQCGLRPLI
                     KYARAMGIEWYVLTDGDDSGKRYADTVKSMEEDFAHRLTMLPQKDIEHFFYKAGLRNV
                     FIQLANWRSQEKYPVSYIIKRAIQRSSKPDLAIALANEMGQRGEHSIPTLFKQLFADV
                     LALINKV"
     gene            14178..15212
                     /gene="metN"
                     /locus_tag="PXD66_000302"
     CDS             14178..15212
                     /gene="metN"
                     /locus_tag="PXD66_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HDL6318637.1"
                     /translation="MIQLKNISKQFEVKGKKITALDNVNLEVPRGTIYGVIGASGAGK
                     STLIRCVNLLERPTIGQVIVDGQDLTAMSDKELIHARRNIAMIFQHFNLLSSQTVFEN
                     VALSLRLSNTPKEQVEKKVNELLDLVGLTDRKDVYPANLSGGQKQRVAIARALASDPH
                     VLLCDEATSALDPATTQSILQLLKDINKRLGLTILLITHEMEVVKRICDRVAVIDKGQ
                     LIESGSVSEIFSNPKTELAQKFIQSTFHFELPQEYTEQLSSSPTEHSKPIIKFEFTGR
                     SVDAPLLSMASKKFGIDFSILMSQIDYAGGVKFGFVIAEVSGNPDSIAEAKFYLIDNN
                     VKVEVLGYVG"
     gene            15202..15879
                     /locus_tag="PXD66_000303"
     CDS             15202..15879
                     /locus_tag="PXD66_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL6318638.1"
                     /translation="MWADFMQEMTPQMWSLVWESTFETVYMGFVATALAVVIGLPIGF
                     LAFLTGKGEILENPRLHQVLDVVINIGRSIPFIILLIILLPFTRLVVGTTLGTTAAIV
                     PLSVSAIPFFARLTSNALLEIPSGLTEAAKSMGATNWQVVTKFYLPESLPILINGITL
                     TLVALIGYSAMAGAVGGGGLGNLAISYGEHRNMVYVKWIATIIIVAIVMISQKLGDHL
                     AKRYDHR"
     gene            15902..16735
                     /locus_tag="PXD66_000304"
     CDS             15902..16735
                     /locus_tag="PXD66_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family lipoprotein"
                     /protein_id="HDL6318639.1"
                     /translation="MSFKKILGVALVSALALTACKEEKKAESTAAPAAQAPAKIKVGV
                     MSGPEHTVAERAAQIAKEKYGLEVEFVLFNDYALPNTAVSKGDLDANAFQHKPYLDKD
                     SQSKGLNNLVIVGNTFVYPLAGYSKKVKNVSELAEGAVIAVPNDPSNLARALILLEKQ
                     GLIKLKDNTNLFSTSVDIIENPKNLKIKEVDTSIAAKALDDVDLAVVNNTYAGQVGLN
                     TQDHGVFVESKDSPYVNIIVARQDNKDAANVQNFIKSYQTEEVYQEAQKHFKDGVVKG
                     W"
     gene            16818..17648
                     /locus_tag="PXD66_000305"
     CDS             16818..17648
                     /locus_tag="PXD66_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family lipoprotein"
                     /protein_id="HDL6318640.1"
                     /translation="MNFKKLLGVALVSALALTACKDEKAQAPATTAKTENKAPLKVGV
                     MTGPEAQMTEVAVKIAKEKYGLDVELVQFTEYTQPNAALHSKDLDANAFQTVPYLEQE
                     VKDRGYKLAIIGNTLVWPIAAYSKKIKNISELKDGATVAIPNNASNTARALLLLQAHG
                     LLKLKDPKNVFATENDIIENPKNIKIVQADTSLLTRMLDDVELAVINNTYAGQAGLSP
                     DKDGIIVESKDSPYVNLVVSREDNKDDPRLQTFVKSFQTEEVFQEALKLFNGGVVKGW
                     "
     gene            17700..18491
                     /gene="metQ"
                     /locus_tag="PXD66_000306"
     CDS             17700..18491
                     /gene="metQ"
                     /locus_tag="PXD66_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HDL6318641.1"
                     /translation="MKIMKLAGAVAIFSLFLTACNDKAEKLKVGVISGPEHKVMEVAA
                     KIAKEKYNRDVELVVFTDYATPNAALDKGDLDLNAFQHKPYLDNQIQEKGYKLVPVGN
                     TFVYPIAAYSKKIKSLAELKDGDTIAVPNDPTNLARALILLEKQDLIKLRADAGLKAT
                     SVDIIENPRKLVIQEIEAPLLPRTLDDVAFSIINTTYAGQNGLTPTKDGIFVEDKDSP
                     YVNLIVARENNQHSEAVKDLVKAYQTEEVYNKANEEFKGAMIKGW"
     gene            complement(18536..19687)
                     /gene="metK"
                     /locus_tag="PXD66_000307"
     CDS             complement(18536..19687)
                     /gene="metK"
                     /locus_tag="PXD66_000307"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018356717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDL6318642.1"
                     /translation="MAINLFTSESVSEGHPDKIADQISDAVLDEILKQDPKARVACET
                     YVKTGMALVGGEITTSAWVDIENLTRQVICDIGYTHSDMGFDAHSCAVLNAIGKQSPD
                     INQGVDRADPLEQGAGDQGIMFGYATNETEVLMPAPITYAHRLMEQQSKVRKSGKLDW
                     LRPDAKSQLTFAYENNKIIGIDAVVLSTQHAEHISQKELIEGVMEEIIKPVLPSEWLS
                     QNTKYFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQLSYAIGVADPTSIMVETFGTGKVSNEILVKLIYQNF
                     DLRPYGLIKMLDLIKPIYRETAAYGHFGRKHFPWEKTDKAEALRAGAGL"
BASE COUNT         5777 a         3956 c         4653 g         5327 t
ORIGIN      
        1 aacatagcta acatatccaa aatcactcac attaggagct taaaaatgac agcgattact
       61 atccctcaat ttgaagaggt gacttctaag caatttcaat ctgagttcgg cagaattgtt
      121 gatatcgtaa aaggcaaaac ccctgtggcg attacccaat atggcagacc gacaatggtg
      181 ctttttcctt acgatcaagc ggttgaagct cggcgattat tagcaaaaca gcgtttgctt
      241 gaaatgcttg cagagcgaga taagaatccg ccggcagctc ttgcagaaat cacgcttgaa
      301 gagttggata aaatgattga tgaggaacgt aatgcactct gattatttag tgattgatac
      361 gaatattttg attagtggtt tattgttccc aaaatcaaat cccaatttag cagtgaaaaa
      421 agccttatta ggctatacca tttgtgtctc gcaagcgact tttgacgaat ttttagaagt
      481 ctccgctaga ccgaaatttg ctaaatattt cagtattcag gaaagggaag aatttattgc
      541 caatttgttg gatgcagtaa agtttgtgga aattaaagaa acgattacag attgccaaga
      601 tcccaaagat aataaatttt tagaagtggc gatcagtgca aaagcgaagt atttagtgac
      661 gggcgataaa aaagatctcc tcagtatgaa cccttatcgg aatgttgaaa ttatttccgt
      721 cagcgaattt ttagcggaaa ctcgctaatg aaacggtttc tcactctttt tcttctcctt
      781 ttttcatctt tttcattagc cgaaacccac caaatcggca ttctcgctca gcgtggggaa
      841 gcctacaccc gcacccattg gcagccttgg gtggattggt taaattcgca gtttcccaac
      901 gagcggtttg agctggttcc attggggatt aacgaggcga gccgccgggc ggagttggat
      961 tttctgctga ccaatcaagc tcagtttttc tatttgagta accaaaatgt gcgatggctt
     1021 gccacgctca acagtccgtt ttcggcaaat ggtgagcaag gggcggtggg cagtagcatt
     1081 tgggtgcgtg ccgacagcca ttatcagcag ttaagcgatc tgaaaaatca gactatcagt
     1141 gcggtggata acgatgcttt cggcggtttt ttgcttgggt tgtatcagtt tcatcaagca
     1201 ggaatgcggc aaaatcggga tttttcggtg cagtttagcg gttttccggt ggaaaatgcg
     1261 ttggctttgc tggcggaaaa acaggtggaa gcggcgattg taccggtctg tttgctggaa
     1321 gagttggaga aagagggcaa gttcaagcgg tcggattttc gcttaatttt gcaaaaccca
     1381 caggcgcagg gttgccttgc cagcacgccg ttgttgccga actggtcgct ggcggcaatg
     1441 gaaaatgtgc caaacgagct ggcggtacag cttgccacac gcttgctaaa cagccacaat
     1501 cccgacctac cccgctggac gttgccgttt tcttccgccc aagccgatca cattctgcgt
     1561 gaactgtatc gccacccgca acaaaaaagc ctgtgggcaa cggtgctgga ttgggttcgt
     1621 ctcaataaat tcggcttgtt ggcggtggtg ttgttcattt tgctcaacct tgtcgccctg
     1681 cgttatcagg tctatcgcaa aagcaaggcg ttacagcagg cacactgcaa aatgcagcag
     1741 tatcagcagg aactgacccg agccgaccgc cttgctttgt tgggcgagat gacaaccggt
     1801 tttgctcacg aactgaaaca gccgttgtcg gcggtgcgaa tgtacgccga ggggctgaaa
     1861 agccaaaaca gcgatcctta tcaacagcgg attttggata agctgattgc gcaggtagat
     1921 cgggcagtga aaacgatgca gagcattcgg gactgggcgc aaaaccgccc gagtggtgaa
     1981 ccgcaagcgg tcattttgaa ccaattgatt actaatgtga tcgagtttgt ggcggtggaa
     2041 aatcgccaga atgcacaaat ctcgctgatt gccgaccgca cttttcggct taatttacac
     2101 gccacggtac tggaacaagt actgacgaac tgcctgctca acgccctgca agccggtgca
     2161 agcgagatat cggtgcggtt acaagcggtc gaaaacgggc tggaaattgc aatcgaagac
     2221 aacggcggcg gtttttcccc tgcccagctt gaatttccgt ttgtgccatt tcgcaccgac
     2281 aaaccacacg gcttagggct aggcttggtg ctatgccaac ggctgatgca atcgctcaac
     2341 ggacggattg tattggcgaa cggggagaac ggggcgaagg tagtgctttt actccctcaa
     2401 atataaatgt aggggcaggt cctgtgcctg cccgtaaatc ctatcaaatt cagggcgggc
     2461 acaggacccg cccctacgga aaaaagaaaa aatgattatt cacattcttg atgacgaaga
     2521 aagcatttta gacgctatgt cgttcctgct tgccccgctt ggggtggaaa tccaaacgtg
     2581 gcaatccagc ctagattttt tggcacaggc ggatttacac cggcaaggcg tgctgttact
     2641 agatattcga atgccgctgc cagacgggca acaggttcat caacaactgc gagaggcaca
     2701 atcgaccctg gcggtggtga ttatgaccgc ccacggtgat gtgccgatgg cagtcgctga
     2761 attgaaaaaa ggcgcagtgg attttctgca aaagccggca agttttgaac agctaaaaca
     2821 ggcgattgca caggcggaaa aagtaagtga aaaagcggtg aaaattcgtg aaatttcgca
     2881 aaattacgcc aaattgacgg aaaaagagcg taaccttgtg ccactgatta tgcaaggttt
     2941 caccaataaa cagatcgcag ataaattagc gatttcagtg cgtacggtgg aagtacatcg
     3001 agccaatgtg atggaaaaaa tgcaggcgga gagtttggcg gggctggtgc agatgatcgg
     3061 ggagttacaa tataaaggta gcggaaattg ctctgctacc tttagtttcg acttttgccg
     3121 tattaattta cttctttaaa ctgaatcatt cccgcttcgg caaaattgtt atcgtcatct
     3181 tcgtataaga aggttttggc ttcaattttg acatcatcaa aagcggtgtc gccttctatg
     3241 ccttcaatgg tttgaccgtg tttaatccct ttaaaatcaa acaatttcgg atcgcataag
     3301 tgggatggca cgatatcttg caaagcactg aacattgttt ctaaacgccc cgggtattgg
     3361 cgatcccaag tattcagcat ttctttcacc acttggcgtt gtaaattagg ctgagaacca
     3421 cacagattac acggaataat cgggaactgc ttggcttcgg catatttgat aatgtctttt
     3481 tctttacaat aggcgagtgg gcggatcaca atttgcttgc cgtcatcaga gatcagttta
     3541 ggtggcatac ttttcatttt gccgccgtag aacatattta agaataaagt cgccagcata
     3601 tcgtcacggt gatgccctaa tgcaattttg gttgcaccca attcggtggc ggtgcggtat
     3661 aaaatcccac ggcgaagtcg tgagcaaagt gaacaggtgg ttttgccctc cggaattttc
     3721 tcttttacga tgccgtaggt gttttcttcc acaattttgt attccacgcc aatgctttct
     3781 aaatagcgag gcaatacctc ttccggaaat cccggttgct tttggtctaa attgaccgct
     3841 accacactaa aattcaccgg tgcactttgc tgtaaattga gcaaaatatc taacagcgta
     3901 tagctgtctt taccgccgga aaggcagacc attacacggt cgccctcttc aatcatacca
     3961 aaatcggcaa tggcattacc gacattacgg cgtaaacgtt tttgtaactt gttgaaatta
     4021 taagtaattt ttttatcaga tttttgaata tcgtctgtca taatagttgt actaggctta
     4081 gaaattagcg gtgtatagta aagatttcgc actattttgc aagttaatta ttcggcttca
     4141 gcgtagcgat atagttacaa ctcacatcat tattgagcca aaatttttcg gttaatgggt
     4201 tgaaatggta gcctctcatc tcttggcaac gtaaatcagc ctgatcgcac caagctaaca
     4261 attcggcagg cttgatgaat ttttcaaatt cgtgcgtgcc ttttggcaac attttcagca
     4321 catattccgc cccgataatt accaacatat aggctttaaa agtacggttg atggtagaga
     4381 aaaacagcac gccgtcaggt ttgagcaggg ctttacagct ttgaatgata gaaagcggat
     4441 cgggaacgtg ttccagcatt tccatacaag taatcacatc gaatttttct tgattacaag
     4501 cggtcatttt tgctaaaaaa ttttcaatcg tggtttgttg gtaatcaatc tctaacccac
     4561 tttctttggc gtgctgcttt gccacttcaa gcggttgaga ggtcatatcg atgccggtaa
     4621 caatcgctcc ctgttttgcc attgcctcac ttaaaatacc gccaccacag ccgacatcta
     4681 acaccttttt gccaaataaa ccgttcgatt tttctaaaat ataggctaaa cgcagtggat
     4741 taagcagatg aatcggcttg aaactgccgt ttggatccca ccaggtttta gccatatttt
     4801 caaattttgt gatttcttgc tgatcgacgt tttgcataaa ctgttcctat ggtaaattta
     4861 gggcattact ataacgcatt tagcctaaaa ggaaaatcag aatgacccga attaaccttg
     4921 ttccgccaac ggaattatgc gatcaacatc tgcttgccga acatcgtgaa cttactcgga
     4981 ttccgaatgc ggtagcgaaa ggcaaattta gcctaaaagg acagccagaa gattacaaac
     5041 tgggcgaggg acacgttcgg tttttcttca ataaactcac gtttttgaaa aagcgttacg
     5101 atgcgttaca cgaggaatgt ttagcaagag gttttaaggt gcaatatttc tgggcggaac
     5161 atttaccaca agatccctct ctctggctag attatcagcc gactgaaacc gctctgactg
     5221 taaatcggga gcgaattgca ttaagaatgc cggcaaatcc acgttttagc gaaagaaaat
     5281 gaccgcttgt acgccttttt gttgcttttt atgctataat ctcgccaatt ttttcgcctt
     5341 atttaaggct cttttactaa taaaattagg aaatttcaat gagcgaatta gccaaagata
     5401 ttattcccgt aagtattgag gacgaattaa aaacctctta ccttgactat gcgatgtcgg
     5461 tgattgttgg gcgtgccttg cctgatgtac gagacggttt aaagccggta caccgccgag
     5521 tgcttttttc aatggatcaa aacagcaaca cctataataa gccgcacgtt aaatctgccc
     5581 gtgtggtcgg tgatgtgatc ggtaaatatc acccacacgg tgactctgcg gtgtatgaca
     5641 ctattgtgcg tatggctcaa ccgttctcac tacgttatat gttggtggac gggcaaggta
     5701 actttggttc tattgatggc gatgcaccgg cagcaatgcg ttataccgaa gtgcgtatgc
     5761 agaaaattac gcaggaatta ttaaccgatc tcgataaaga aacggtggat ttctcaccga
     5821 actatgacgg caaagagatg attcctgatg tattgccaac caaaattccg gctctgttag
     5881 tcaatggttc ttcagggatt gcggtcggta tggcaaccaa tattccacct cataatttag
     5941 gcgaagtatt agatggttgc ttggcgtata ttgaaaatga acagattagt gttgaagagc
     6001 taatgcgtta tatcccgggg ccggatttcc cgaccggggc gattatcaac ggtcgtaaag
     6061 ggattgaaga ggcttaccgc accggtcgtg gcaaggtgta tgtgcgcgcg aaagccagcg
     6121 tggaaacaac tgataaaggg cgtgagcaaa ttgttgtaac cgaactgcca taccaagtga
     6181 ataaggcaaa attaattgaa aaaattgccg agctggttaa agagaagaaa atcgaaggca
     6241 tcagcaaaat tgatgactat tccgataaaa aagggatttc gattgttatt gaagtcaagc
     6301 gtgatgcagt gggtgaagtg gtgttaaacc acctttattc tttaacccaa atgcaggtca
     6361 cgtttggtat caatatggtg gcgttagata atgggcagcc aaaagtgctg aacttacgcc
     6421 agatcatcga agcctttgtt aaacaccgtc gtgaagtggt cacacgccgt acgatttttg
     6481 aattgcgtaa agcccgtgaa cgtgcccata ttttagaagg tttggcgatt gcgttagcga
     6541 atatcgaccc ggtaattgaa ttaattcgag cctcaaaaac cgcagaagaa gcccgtgaag
     6601 gcttattggc tcgcccttgg gcattaggca atgttgcacc aatgttagag gctgctggtg
     6661 ttggtgcttc ccgaccggaa gatttacctg aacacttagg agtgcgtgat ggcgaatact
     6721 atttatctga agctcaagcc agagcgattt tagagctacg cttacaccgc ttaaccggtt
     6781 tagaacacga gaaaatcgtt gaagaataca aagaactctt aactgaaatt ggcgaattaa
     6841 tccacatttt aaccagccca gaacgcttaa tggaagtgat ccgtgaagag ttagaacgtg
     6901 taaaaaccga ttttaatgat gaacgccgta ctgaaattac cgcagcctct ggcgacatca
     6961 acattgaaga cttaatcgca caagaagatg tggtggttac gctttctcac gccggctatg
     7021 tgaaatatca gccgttatcc gactacgaag cacaacgccg tggcggtaaa ggtaagtcgg
     7081 caaccaagat gaaagaagat gatttcatcg aaaaactctt ggtggcaaac acccacgaca
     7141 ccattctttg cttctcaagc cgtgggcgtt tatatcaatt aaaagtgtat caattaccgc
     7201 aagcaagccg tggggcaaga ggcacgccga ttgtgaatat tctgccgtta gtgaaagaag
     7261 aaaacgaacg tattaccgcg attctaccga ttccaaacgg tgaatttagt gccgataagt
     7321 ttatctttat ggcaacagct agcggtgtag tgaaaaaagt ttcccttgat gcctttagca
     7381 acgtgcgtgc aagcggtctg attgcgttaa aacttcgtga aggcgatgag ctgatcgggg
     7441 tagatattac tgatggtaac agcgaaatta tgttgttctc ggcacaaggg cgagtagttc
     7501 gctttgccga aacagcagtt cgtgcgatgg gacgtaccgc caccggcgta cgaggtatta
     7561 aactttctac caccgaaatt tcaagcgaag aaattgaaga agctgaaatt attgaaactg
     7621 acaatgaaga ggcagaagat tcctccagcg tcagccttga taaagaccgt gtggtttctt
     7681 tagtgattcc tcgcaccgaa ggcgatattt taaccgtcac ccaaaacggc tacggtaaac
     7741 gtacggtgat tagcgaatat ccggtgaaat cccgagcaac caaaggggtc gtttcgatta
     7801 aagtgaatga acgaaacggc aaagtggttg cggcagttca agtagaggaa accgaccaaa
     7861 ttatgttgat taccgatgcc ggcacgctgg ttcgcacccg agtggcagaa gtcagcctcg
     7921 ttggacgaaa cacccaaggc gtacgtatta ttcgcaccgc agaagacgaa caagttgtta
     7981 gccttgaacg agtctgcgag cctgaagacg atgaaaatga aagcctagac gcattagaaa
     8041 atgccgaaac agcagaaaca tcaactgaag aataatctta actaaacaag cggtcgaatt
     8101 ttcacaattt tttgcaaaaa tagaaggaaa ttcgaccgtt tttggatcat acagtagata
     8161 ataaaaacta tggcacttat caatctttcc aacgcctatt taggctttgg cgatcatcct
     8221 ttattggatc atactgaatt acatatcgaa cccggcgagc gggtttgttt ggtggggcgt
     8281 aacggggcgg gtaaatcgac cctgatgaaa attctggcgg gcgaagttca gcttgatgac
     8341 ggcaagttgg tatttgaaaa agacattgtc gttacacgtc ttgaacaaga tccgccccgc
     8401 catattgaag aaacggtgtt cgattatgtg gcggagggga ttgcccatct tgccgactta
     8461 ttgaagcaat atcaccacat ttccctgcaa atgcagaccg attacagcga tgaactgctc
     8521 gccaaacttt cagcggtgca atcgcaatta gaacacgcta acggttggca gtttgaaaac
     8581 cgcattcaag acaccttgaa actgctggaa ctcgcccccg acaaacgcct gtgcgaactc
     8641 tcaggcggtt gggtacgccg tgcggcattg gctcgggcgt tggttgccga tcctgatgta
     8701 ttgctgttgg acgaaccgac caaccacctc gacattgagg cgattgcgtg gctggaaaat
     8761 ctgttattgg atttcaaagg cagtattatt tttatctcgc acgaccgtgc ctttatccgc
     8821 aaaatggcga ctcggattgt ggatttagat cgaggcaaat tggtttccta cccaagcaat
     8881 tacgataaat acttggaaga aaaagccgag gatctgcgag tagaagcgtt gcaaaatgag
     8941 ctgttcgaca aaaaactcgc ccaagaagaa gtctggatcc gtcaagggat taaggcaaga
     9001 cgcacccgaa acgaagggcg tgtgcgagcg ttgaaagcgt tgcgtgaaga gcgtagaaac
     9061 cgccgagagg tacaaggcac ggcgaaaatt cagatcgaca acaccgcacg cagcggcaaa
     9121 attgtgtttg aagtggaaaa tgccggctat gaaatcggcg gcagaaccct gctgaaaaat
     9181 ttcagcacca ccattttgcg tggcgataaa attgccctga ttggagcgaa cgggatcggc
     9241 aaaaccacgc tgatcaagct gttacttggc gaaattcagc cgacatctgg ttcagtgcgt
     9301 tgtggcacaa aattggaagt ggcgtacttc gaccaatacc gagtcgatct tgaccctgaa
     9361 aaaaccgtga tggacaacgt ggcagacggc aagcaagatg tagaagtaaa cggggtgaaa
     9421 cgccacgttc ttggctatct gcaagacttc ctgttcccgc caaaacgggc gatgacaccg
     9481 gtgaaagccc tctcgggtgg tgaacgcaac tgcttgttgc tggcaaaact gttactcaaa
     9541 ccgaacaact tgctgatttt ggacgaaccg accaacgatt tggacgtgga aaccctcgaa
     9601 ctgctggaag agatgctcac cgactatcaa ggcaccctga ttattgtgag ccacgaccgt
     9661 caatttgtgg ataataccgt aaccgaatgc tacttcttcg agggcgaagg cgtggtgaat
     9721 aaatatgtcg gcggctattt cgatgccaaa caacagcagg aaaattactt cgccgccact
     9781 gccgacaagc ggttaaattc gagtaaaaat ttgcaaaaaa tagaggaaaa agcaccgctt
     9841 gcggagcaag agaaacagaa agccgatcag gaaaaaacga aagcagagcc gacgaagaaa
     9901 accaaacttt cctacaaaga acagcgtgaa ctggacgagt taccggctaa aatggaacag
     9961 cttgaagccg acattgaagc cctgcaagcc gaagtaaata gtgccgaatt cttcgccaaa
    10021 gatccaagct acaccaacgc ccagctacaa aaacttgccg ataccgaagc cgcgttagag
    10081 caagcctttg agcggtggga agcgttggaa aatattaaaa acgggaatat ctaatgtagg
    10141 ggcgaatgat tattcgccct tgatggataa aatgattggg cgaaaaattt ttcgccccta
    10201 caaatgtgaa gcaaaatgac agaaagatta gataactacg aacaacgttt tggtggcatt
    10261 ggtcgccttt atacgccgga aggattggag aaactacgcc gttcgcatat ttgcgtgatt
    10321 gggattggcg gggttggctc gtgggcagtg gaggcattgg ctcgctcagg tatcggtaag
    10381 atcacgatga tcgatatgga cgatatttgc gtgaccaata tcaaccgcca aattcacgca
    10441 atgagtggca cggtggctca actgaaaacc gaagcgatga aagagcggat tgagcggatc
    10501 aaccctgaat gtgtggtgga aattattgat gatttcttaa cgcaggataa tctcgcagac
    10561 tacctcaatc gaggatatga ctatgtgatc gatgcgattg acagtttgcg ggtaaaagcg
    10621 gcgatgattg cgttttgtaa acgcaataag atcaaaatta tcaccaccgg cggggcgggc
    10681 gggcagaccg atccgaccaa aattcaaatt accgatttaa gcaaaacggt gcaagatccg
    10741 ttggcggcaa gggttcgtag cctgttgcgt aaagagtacg gtttcagcaa taaaaacaaa
    10801 ttcggcgtgg agtgcgtttc ttcgactcag caactgattt tcccacaaat gggtgaggcc
    10861 tgtgaggtgt cggcaacaat gaactgtgcc aacggctttg gagcggtgac aatggtgacg
    10921 gcaacgttcg gcttttttgc ggtgagtcgg gtgattgata aactattaaa ataacaagcg
    10981 gtcatattaa atgaattttt tgcaaatcaa ggagtaaata atggcaatac atcgagtcaa
    11041 cccgaaaggg agtatggaac agctttccca tttggaaatg gagttgttgg caaagaatac
    11101 gcagggtaat ttgcatcaac tttatcgtaa ttgctctttg gcggtgctaa attccggtgt
    11161 gcataccgat gacagccgag cgttgttaag ccaataccct gattttgaaa tccgcttatt
    11221 aacccgtgaa aaaggcgtgt cgcttgaatt gcataatcca cccgaaactg cgtttgttga
    11281 cggcaagatg attttgaata ttcaatatca cctttttgcg gtgctgcggg atattgtgtt
    11341 tgtgaatgcg ttaaaaaatg cgattagacc gcttgaagag acttctttag aagccttaac
    11401 cacgaatacc gtattttcga ttttgcggaa tgcgaaagcg attgaaatga ataccgaccc
    11461 aaatttggtg gtgtgctggg gagggcattc gattaatgaa accgaatacc aatattgccg
    11521 tgccgtcggg caagagtttg gcttgcgtga gctgaatatt gtgaccggtt gcggagccgg
    11581 tgtgatggaa gccccgatga aaggagcggc tatcggacac gccaatcaac gttataaaaa
    11641 cgggcgtttt atcggcatta ccgagccgtc tattattgcc tccgagccgc caaacccgat
    11701 tgtgaatgaa ttggtaatta tgccggatat tgaaaaacgg ttggaagcct ttgtgcgttt
    11761 agggcacgcc attgtggttt tcccgggcgg gccgggaacg ctggaagagt tgctgtatat
    11821 tatcggtatt aagttaaacc cggctaacca tgctcagcaa ttaccactga ttttaaccgc
    11881 cccgaaagaa tcggcagact atttctatgc catcgatcaa tttattggcg aaactttagg
    11941 cgaagaagct cgcagtttgt atgaaattat tattgatgac tcggtgttgg tggctcgtaa
    12001 agtcaagcaa gcgatgattg aggtaaaaag cagccgttat gaaagtggcg atgcttataa
    12061 tttcaactgg tcgttaaaaa ttgaggaaga ttttattacg ccatttattc caacccacga
    12121 aaatatggca aacctgaatt taagtttagc acagccaaaa gagcagctgg ctgcgaattt
    12181 aagacgggca ttttcaggga ttgttgcagg taatattaag ccggagacct ccgatttaat
    12241 tgagaaatat ggggtgttta aattacacgg cgatcgtaaa ttaatggagc atatagataa
    12301 actgttggag cgttttgtgg ttcaacaccg tatgaaaatt ccaacgggtg aggcgtacca
    12361 accttgctat gaaattgtaa aagggtaatg ctaacaaggg gtaaggtatg tatttaaagc
    12421 atattgaaat cagtggcttt cgtggtatta accatttatc gctccagcta cgaccaaata
    12481 tggtattgat tggcgaaaat gcgtggggga aatccagtct gattgatgca ttaagcctga
    12541 tttttaacct tgagcaggcg ttgtatcaat ttcagttcaa agatttccat attcaataca
    12601 ccgatacccc caaccaagtg cgggacattc aactggtgtt taccttttgt gaagagcatc
    12661 agcacgattt agcggtgcaa tttcccgctt atttggcaca agccgcgttt tatattgata
    12721 atcaccgctg tgtttcgctt gccgtaaccg gaaaattaca cggagagcag attagcaccg
    12781 actatcaatt tgtggatcaa agcctaaaac cgatccagat agaacaagcg gaaaacgcca
    12841 ttcggcagtt tatttaccat caccctgttt atcgcctgcg tgatgcaagg ctcaataaca
    12901 gggtgcataa tgaattatta cttaaaggat cgtctcaact tgaagacgat tttcaacgtg
    12961 aattaaacgc cctctcttcg ctactgaaat actattttct aaccagccaa agtcgctatt
    13021 tgatcaaaac cagtatgcaa gacaccgcat tgtggtggga aaaagtaaaa tccctctgtt
    13081 tacgcctaaa acagcagccg gaattggtcg aatctgctca gcagcatatt tctactctat
    13141 ttttgtttaa tcctagatct acagtaatag aaaagccgat tattttattt gaagatctga
    13201 atgctcaact acaccctcga atgattgcga ttttttggga actgctcggt tttttaccca
    13261 gccaacggat caccactact aattctattg aattgttatc gcaagtgcct ctgcgtgaaa
    13321 tctgccgttt ggtgcgtcat cacgatcata cccaagccta ttggcttgaa cgccacagtt
    13381 taggcaagga agatttgcga aaattaacct ttcatattca ccataatcgt ggcttggcac
    13441 tgtttgctaa agcgtggatt ttggttgagg gggaaacgga ggtttggatt ttgcaagagc
    13501 tggcgagatt attagatatt catttggaaa tggaagggat caaaatcgtt gaatttgctc
    13561 agtgtggctt acgtccgtta attaaatacg ccagagcaat ggggatcgaa tggtatgtgc
    13621 tgactgacgg tgacgattcc ggcaaacgtt atgcagatac ggtaaaaagt atggaagagg
    13681 attttgctca ccggctcacg atgttgccac aaaaagatat cgaacatttt ttctacaaag
    13741 caggcttgcg gaatgtgttt atacagcttg caaattggcg ttcacaagaa aaatatccgg
    13801 tgtcctacat cattaaacga gcgattcaac gaagctcaaa gcctgattta gccattgcat
    13861 tagccaatga gatggggcaa cggggagagc attcaattcc aactttattt aaacaattat
    13921 ttgcagatgt gctggcattg ataaacaaag tatagaatac aagcggttaa atttgacaaa
    13981 aaattgccaa ttttgaccgc ttgtttgctt gtgatgaaaa ccaatcatac aaaactctaa
    14041 tttatttcaa gtgcaagcta ttattttatg cgttatgctt atttggaata aaaaaatcgg
    14101 gttagttgat tgacatagca ttctagacgt ctaaaataac caaaaataat agttaattga
    14161 atgtggataa atagaaaatg attcagctga aaaatatcag caaacaattt gaggtaaagg
    14221 gcaaaaaaat taccgctctg gataatgtaa acctagaagt accaagaggc actatttatg
    14281 gtgtcattgg ggcatcaggg gcaggaaaaa gtaccttaat tcgctgtgta aatttattag
    14341 aacgcccaac aatcgggcaa gtgattgtcg atgggcaaga tttaacggca atgtcggata
    14401 aagagttgat tcacgctcgc cgtaatattg caatgatttt ccaacatttt aatttgcttt
    14461 catcacaaac tgtatttgaa aacgtggcgt tatcgttgcg tttaagcaat acgccaaaag
    14521 agcaagttga gaagaaagtc aatgagctgt tggatttagt ggggttaaca gatcgtaaag
    14581 atgtttaccc tgcaaatctt tccggtgggc agaaacagcg tgtagcgatt gctcgtgcgt
    14641 tagccagcga tccacacgtt ttgttgtgtg atgaagcgac cagtgcgtta gatccggcga
    14701 ctacgcaatc cattttgcag ctattaaaag atattaataa acgtcttggt ttgacgattt
    14761 tattaatcac tcacgaaatg gaagtggtaa aacgcatttg tgaccgagta gcagtgattg
    14821 ataaagggca gttgattgaa tctggctcgg taagtgaaat tttctcgaat ccgaaaaccg
    14881 aattagctca gaaatttatt caatcgactt tccattttga attaccgcaa gaatataccg
    14941 agcagctttc aagttcgccg actgaacata gtaagccgat tattaaattt gagtttaccg
    15001 gacgttcggt ggatgcacct ttgctttcaa tggcatcgaa gaaattcggc attgatttta
    15061 gtattttgat gtcgcaaatt gattatgcag gcggggtcaa atttggattt gtaattgcag
    15121 aagtgagtgg taatccggat tccattgcag aggcaaaatt ttatttaatt gataacaatg
    15181 taaaagtaga ggtgttaggc tatgtgggct gattttatgc aggagatgac gccacagatg
    15241 tggagcttag tatgggaatc tacttttgaa accgtttata tgggctttgt ggcaacggca
    15301 ttggcggtag tcatcggttt accgattggt ttcttggcgt ttttaaccgg taaaggcgaa
    15361 attttagaaa atccgagatt acaccaagtg ttagatgtcg ttattaacat cgggcgttcg
    15421 attccgttca ttattttatt gattattttg ttgccattta ctcgcttagt tgtgggcaca
    15481 acattaggca caacggcagc gattgttcct ttaagtgttt cggccattcc gttttttgcc
    15541 cgtttaactt caaatgcgtt gcttgaaatt ccgtccggtt taacagaagc ggcaaaatca
    15601 atgggagcaa caaattggca ggtagtgaca aaattttatt taccggagtc attaccgatt
    15661 ttgattaatg gcattacgct aacgctggtt gctttaattg gctactcagc aatggcgggg
    15721 gctgttggtg gcggcggttt aggcaattta gcgattagct atggcgaaca ccgtaatatg
    15781 gtgtatgtga aatggattgc taccattatt attgttgcga ttgtgatgat tagccaaaaa
    15841 ttgggtgatc atttagcaaa acgttacgat catcgttaat tttatttaaa aagaggtttc
    15901 tatgagtttc aagaaaattt taggcgttgc attggtttct gcattagcat taaccgcttg
    15961 taaagaagag aaaaaggcag aatctaccgc tgctccggca gctcaagctc cggctaaaat
    16021 caaagtaggg gtaatgtcag gccctgagca taccgttgca gaacgtgcag cacagattgc
    16081 aaaagaaaaa tatggtttag aagttgagtt cgttttattt aacgactacg ctttaccgaa
    16141 tactgcagta agtaaaggtg atttagatgc aaacgcattc caacacaaac catatttaga
    16201 taaagacagc caatcaaaag gtttaaacaa cttagtgatt gtgggtaaca cctttgttta
    16261 tccgttagcg ggctactcta aaaaagtgaa aaatgtgtct gagttagcag aaggtgcggt
    16321 aattgcagta ccaaatgatc cttcaaactt agctcgtgct ttgattttat tagaaaaaca
    16381 ggggttaatt aaattaaaag ataataccaa tttattctca acttcagtgg atattatcga
    16441 aaatccgaaa aacttaaaaa ttaaagaagt ggatacttca attgccgcaa aagcgttaga
    16501 tgacgtagat ttagcggtgg taaataacac ttatgccggg caggtaggct taaataccca
    16561 agatcacggt gtatttgttg agtctaaaga ttcaccgtat gtgaatatta ttgtggctcg
    16621 ccaagacaat aaagatgcag ctaatgtaca aaactttatt aaatcttacc aaaccgaaga
    16681 agtgtaccaa gaagcacaaa aacactttaa agatggtgta gtaaaaggtt ggtaatttag
    16741 ctgattttta cttaaatggc gattaagcgc taggttaatc gccattttca tctttaaaat
    16801 ataggaagga gcaaacaatg aactttaaaa aattattagg tgtggcgtta gtatctgcct
    16861 tagcacttac tgcgtgtaaa gatgaaaaag cacaagcacc tgctacaaca gctaaaactg
    16921 aaaacaaagc cccattaaaa gtgggtgtga tgaccggccc tgaagcccaa atgaccgaag
    16981 tggcagtgaa aattgcaaaa gagaaatatg gcttagatgt agagttagtg cagtttactg
    17041 aatacactca accaaatgcc gcacttcatt ctaaagattt agatgctaac gcgttccaaa
    17101 cggtgcctta tttagagcaa gaagtgaaag atcgtggtta taaattagcg attatcggca
    17161 atacgctagt atggccaatc gcggcttatt ctaaaaaaat taaaaacatt tccgagttaa
    17221 aagacggagc gactgttgcg attccaaaca atgcaagtaa tactgctcgt gcgttattat
    17281 tgcttcaagc tcacggttta ttgaaattaa aagatccgaa aaatgtgttt gctaccgaaa
    17341 acgatattat cgaaaacccg aaaaatatca aaatcgtaca ggcggatacc tcacttttaa
    17401 cccgtatgtt agatgatgta gaacttgcgg taatcaacaa cacttacgca ggtcaagctg
    17461 ggttaagtcc ggataaagac ggtattattg tggaatctaa agattcaccg tatgtgaatt
    17521 tagtggtaag tcgtgaagat aataaagatg acccacgctt acaaactttt gtgaagtcat
    17581 tccaaaccga agaagtattc caagaagcgt taaaattatt taacggtggt gtggtgaaag
    17641 gttggtaatc agttggacgg catattctgc cgtccttttc ttaaaaggag aaacagataa
    17701 tgaaaataat gaaattagcc ggtgcagttg caattttctc gcttttttta accgcttgta
    17761 atgataaagc cgaaaagttg aaagtcggtg tgatttccgg ccctgaacat aaggtaatgg
    17821 aagtggcggc aaaaattgca aaggaaaaat ataaccgtga tgttgaatta gtggtattta
    17881 ccgattatgc cacgcctaat gcagctttag ataaaggcga tcttgatttg aatgctttcc
    17941 agcataaacc ttatttagat aaccaaattc aggaaaaagg ctataaatta gtgccggtcg
    18001 gcaatacttt tgtttatccg attgcggctt attccaaaaa aattaaatcg ctggcagagt
    18061 tgaaagatgg tgatactatt gcagtgccta atgatccgac taatttagcc cgtgctttaa
    18121 ttttattgga aaaacaagat ttaattaagc tgcgagcaga tgcaggctta aaagcaacca
    18181 gtgtggatat tattgaaaac cctcgtaaat tggtgatcca agaaattgaa gcaccattat
    18241 tgcctcgaac gttggacgat gttgcctttt cgattattaa tactacctac gcagggcaaa
    18301 acggtttaac gccaaccaaa gacggaatat tcgttgaaga caaggattcg ccttatgtga
    18361 atttaattgt tgcccgtgaa aataaccaac attctgaagc cgtaaaagat ttggtgaaag
    18421 cctaccaaac agaagaggtg tataacaaag cgaatgaaga gtttaaaggg gcgatgataa
    18481 aaggctggta attgaagata aataaggcat agattcctat gccttacttt ttatgttaca
    18541 gacctgctcc ggctcttaat gcttcggctt tgtcagtttt ttcccaagga aaatgtttac
    18601 gaccgaagtg tccgtaggca gcggtttcac ggtagattgg tttgattaaa tctaacattt
    18661 tgattaagcc atacgggcgt aagtcaaagt tttggtagat taatttcaca agtatttcgt
    18721 tgctcacttt acctgtgccg aaggtttcga ccataattga tgttgggtca gccaccccga
    18781 tagcataaga aagttggatc tcacagcgat cggctaagcc tgcggcaacg atattttttg
    18841 cgacataacg agcagcataa gcagcagagc gatccacttt tgacgggtct ttaccagaga
    18901 atgcaccacc accgtggcga gccgcaccac cgtaggtatc tacaatgatt ttacgcccgg
    18961 ttaaaccaca gtcacccatt ggaccgccaa tcacaaaacg accggttggg ttgatgaaat
    19021 atttggtgtt ttggctgagc cattcgcttg gtagtaccgg tttgatgatc tcttccatca
    19081 ccccttcaat taactctttt tgcgaaatat gctctgcgtg ttgtgtagaa agcactacgg
    19141 catcaatgcc gataatttta ttgttctcat aagcaaatgt taattggctt ttagcgtcag
    19201 ggcgtaacca atcgagtttg cccgatttac gcacttttga ctgctgttcc attaagcggt
    19261 gtgcgtaagt aattggagcc ggcattaaca cctcggtttc attggtagcg tagccaaaca
    19321 taatcccttg gtcgcccgca ccttgctcta acggatcggc acggtcaacg ccctggttga
    19381 tgtccggaga ttgtttacca atcgcattta ataccgcaca agagtgagca tcaaagccca
    19441 tatcagagtg agtatagccg atgtcgcaaa tcacttggcg ggttaagttt tcaatatcca
    19501 cccacgcaga ggtagtgatt tctcccccta ctaatgccat accggttttc acataagttt
    19561 cacaagcaac gcgtgcttta gggtcttgtt ttaaaatttc atctaatacc gcgtctgaaa
    19621 tttggtctgc gattttatcc ggatgccctt ctgaaaccga ttcagaagta aataaattaa
    19681 ttgccatttg tattcctctc tatcttaaat atg
//
LOCUS       DANLXF010000014        19666 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.014, whole genome shotgun sequence.
ACCESSION   DANLXF010000014 DANLXF010000000
VERSION     DANLXF010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 19666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19666
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.014"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..846
                     /gene="prmC"
                     /locus_tag="PXD66_000308"
     CDS             <1..846
                     /gene="prmC"
                     /locus_tag="PXD66_000308"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDL6318643.1"
                     /translation="TFAEQKLLENSGADPYLNAKFDANLLLQAVTKRSKSAIFAFSET
                     LLSESELAELAEKLARRAKGEPMAYILGYREFWSLPLKVSPATLIPRPDTERLVELAL
                     EYANKRLGNQKMLQILDLGTGTGAIALALASELGEKAQIIGVDFQNEAVQLAEENRRN
                     LGLNQVTFLQSDWFSSLANQQFDLIVSNPPYIDESDENLTVGDVRFEPLTALVADNKG
                     LSDLQKIIENAPLHLKPQGALMLEHGWKQGNLVRDLFDLHLWEQVETAQDYAGNDRVT
                     KAILK"
     gene            855..1721
                     /locus_tag="PXD66_000309"
     CDS             855..1721
                     /locus_tag="PXD66_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB1 family protein"
                     /protein_id="HDL6318644.1"
                     /translation="MDDIIEELLKELEDPKVTISETELKKFLYREILRLTTLIDKNVS
                     EQQVFGQMSALVKKARYNVNGEGDEERINQLLHLVYREWGFHCYYEDYFHTENLLLNQ
                     VIRKRRGMPVSLGAVALYLSAVLDLPLYPVNFPTQLVLRAEIHQPNGATKVRFINPWN
                     GEFLTIEMLNKWLEGEMGFGVEVTPDLLRRAEPQELLERVETVFKMALTREGKYEETL
                     RLIEYRLAFSPEDPYEIRDRGMVLASMDCYQAALEDINYFIDQCPEDPSAEILKMEVK
                     GLQQKSQESVVH"
     gene            1811..2665
                     /gene="kdsA"
                     /locus_tag="PXD66_000310"
     CDS             1811..2665
                     /gene="kdsA"
                     /locus_tag="PXD66_000310"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012073169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HDL6318645.1"
                     /translation="MTDKIVKVGNIEVANHKPFTLFGGMNVLESRDMALRVCEQYVEV
                     TQKLNVPYVFKASFDKANRSSIHSYRGPGMEEGLKIFQELKQTFGVNIITDVHEIYQC
                     KPVAEVVDVIQLPAFLARQTDLVEAMARTGAVINVKKPQFLSPGQMGNIVEKIAECGN
                     DQVILCDRGTNFGYDNLVVDMLGFGIMKKVSKGCPVIFDVTHSLQCRDPFGAASGGRR
                     DQVTELARSGMAIGLAGLFLEAHPDPNSAKCDGPSALPLSKLEAFVSQMKAIDELVKS
                     FEEIDTSK"
     gene            2886..3833
                     /locus_tag="PXD66_000311"
     CDS             2886..3833
                     /locus_tag="PXD66_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HDL6318646.1"
                     /translation="MLNIVFLDRTGIPATHDIPRPNFPHTWTEYATTSADQTFERVKE
                     ADIIITSKVLLQRDLLAKLPKLKLIAITATGTNNVDLAAAKELGIAVKNVTGYSSVTV
                     PEHVIGMIYSLKHRLADYHRDLITSDRWATCGQFCYVDYPIHDVRGSTLGIFGKGNIG
                     AEVARLAQAVGMQVIFAEHQGATEIREGYTAFEEVFKQADIISLHCPLTEQTQNLINA
                     ETLALMKPTAYIINTGRGPLIDEQALLEALEQGKIAGAALDVLVKEPPEKDNPLMLAA
                     KRLPNLLITPHVAWASDSAVTTLVNKVTQNMEEFVATGM"
     gene            3931..5103
                     /locus_tag="PXD66_000312"
     CDS             3931..5103
                     /locus_tag="PXD66_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HDL6318647.1"
                     /translation="MNLTPFFIAFRYFRSKSADRFGRLVTNLASLGIVLGVMALVIVL
                     SVMNGLENMQKTNLLSTLPHAIITPQEGHFAKGEKLALPEFAKQSVVINRANVIIQSS
                     QGINAGQLIGVENATDDPLLVAENIPALLPSGEFKVVIGSRLANKLNVTVGDKLRLMI
                     TENSQYTPLGRVPVQRLFEISGVYQSNREASEFTLFANLADVGRLLRISEDQVQGVRL
                     YLQDPFQVTELSQYFDAERYQISDWREQKGEFFQAVKMEKNMMGLLISLIIVVAISNI
                     VTSLSLMVVDKQGEIAILQTQGLTKKQVMQIFVFQGAIVGVIGSIIGGLLGVLIAYNL
                     DQIILLLNPSIHLPTLISGTQIAVIVGVSMLLSLLCTLYPAYRASKIEPAQALRYE"
     gene            5120..5803
                     /gene="lolD"
                     /locus_tag="PXD66_000313"
     CDS             5120..5803
                     /gene="lolD"
                     /locus_tag="PXD66_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HDL6318648.1"
                     /translation="MTELLRCEHISKFYDEGEQKVQVLKEVSFSMNEGELVAIVGSSG
                     SGKSTLLHTLGGLDQPSTGEVWIRGQSLQQLSSDKLALLRNQNLGFVYQFHHLMADFT
                     ALENVMMPMLIGKQNKTEAANRAEKMLQAVGLAHRMTHRPSALSGGERQRVAIARALV
                     NNPALVLADEPTGNLDQKTTESIFELIQQLNQEQNIAFLLVTHDLNLANKLSRSLVMR
                     DGVLSEGSL"
     gene            5800..7065
                     /gene="lolE"
                     /locus_tag="PXD66_000314"
     CDS             5800..7065
                     /gene="lolE"
                     /locus_tag="PXD66_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HDL6318649.1"
                     /translation="MSTPFFISWRYQRGKQKNKLVSLIALFSNIGIALGVAVLIIGLS
                     AMNGFERELNNRVLSVVPHAELVSFQGNQPQPIFDSKKLEALIKKSQNVTASSPFVSF
                     TALIENGAQLKIAQVRGVDPKKQDQASQLSQFIPPEQWQAFNQQFQADEDGLILGAGI
                     AKALEVEAGDEVTLLIPQPTEDGKLAQPLRFNLNVTGILRLDGQLDHSYALIPLNKAQ
                     ELMEYQANEVSGLEISLANPFDVQQLEMPQLNGYTQPLYLNTWIDKFGYMYNDIQLIR
                     TVMYIAMVLVIGVACFNIISTLIMAVKDKQGDIAILRTLGANNGFIRRIFLWYGLISG
                     MKGALFGLILGVILSLNLTAIIKAIEAFFEVKLLSDGVYFVDFLPSELHWQDVVYVLA
                     ATIILSLFASLYPANRAAKLEPAKVLSGH"
     gene            complement(7140..9071)
                     /gene="thrS"
                     /locus_tag="PXD66_000315"
     CDS             complement(7140..9071)
                     /gene="thrS"
                     /locus_tag="PXD66_000315"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDL6318650.1"
                     /translation="MPIITLPDGSQRSFDNPVSVMEVAQSIGAGLAKATIAGRVNGER
                     RDASDIISQDSTLEIITAKDEDGLEIIRHSTAHLLGHSIKQLFPDVKMAIGPTIENGF
                     YYDIDLDRSLTQEDLDALEKRMLELAKTNYDVVKRPVSWQEARDTFEKRGEPYKMAIL
                     DENIERTATPALYHHEEYIDMCRGPHVPNMRFCHHFKLQKVAGAYWRGDSKNKMLQRI
                     YGTAWADKKQLAEYLQRLEEAAKRDHRRIGKALDLYHMQEEAPGMVFWHNDGWTIFRE
                     LETFVRTKLKEYDYQEVKGPFMMDRVLWEKTGHWQNYADLMFTTQSENREYAIKPMNC
                     PGHVQIFNQGLKSYRDLPIRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IESEVISCIKMVYDIYSTFGFTNIAVKLSTRPENRIGDDAMWDRAEKGLANALRNNGL
                     EYEIQEGEGAFYGPKIEFALRDCLGREWQCGTVQLDFALPGRLAASYVAEDNGRRTPV
                     MIHRAILGSIERFIGIITEEYAGFFPTWLAPTQAVVMNITDSQADYVQQVVKQLSDAG
                     IRVKADLRNEKVGFKVREHTLRRVPYMLVCGDKEIAEGKVSVRTRKGADLGTYLVSDL
                     VEILKNQIKARELKLLGEE"
     gene            complement(9238..10587)
                     /locus_tag="PXD66_000316"
     CDS             complement(9238..10587)
                     /locus_tag="PXD66_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL6318651.1"
                     /translation="MNSTKFYSLYSGIFFLFFGYGLFLNSAGIKLAEMGITDIIIGLL
                     NAAFFIGAALSTIFAHRIVSSVGHIRSFSVFCAVFAIAALGHIMIDTLWIWGVLRIIL
                     GFCHYSLLLLVESWLSEKTNVDTRGKVLATYNIIFYLAFILGVSLLSLELSSRNIFIL
                     STILVVMSMIPIALTKMVQPDIPARERISIPKLFAISPLALATSFISGMLVNGFFTMA
                     SVFLLKFSFSLSEISLYLMISMIGGFISQMPMAILSNRIGRRNAILACSVIATLTALA
                     GLFAIFQENLTAWGQYIIAFFFGCSLFSLYALSIARANDELPNNMNTVEVSRGLLFCY
                     GVGALVAPPLIGFAMSLSAEYGFYALFGLFSLSLFICAFRTKSIPKAERAEMQYATPI
                     ATASPLEEIEVVEDALIPFDEEAVQEYQAQLEILEQDPQTENENIELDEGAAENSKV"
     gene            complement(10862..11353)
                     /locus_tag="PXD66_000317"
     CDS             complement(10862..11353)
                     /locus_tag="PXD66_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDL6318652.1"
                     /translation="MFTTQLIQTKRWLEANSITTFWLKTFIGATLLAIFAQISVPMYP
                     VPITGQTLALTVVGFAMGRKAATAAVLLYLFEGAIGLPVFAGGASGFHHFFGPTGGYL
                     LGYIPTAYFLGYYSDKGVLNSFWKSLFVALIASVITFAFGLAQLSLYQKAQYYKRAYI
                     HLF"
     gene            11434..12201
                     /locus_tag="PXD66_000318"
     CDS             11434..12201
                     /locus_tag="PXD66_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--protein ligase"
                     /protein_id="HDL6318653.1"
                     /translation="MVKPVYIYCDEGSSEIGVQSLLLAVKQHLQRPAYLINAKQIIQS
                     KFSDGCLLIMPGGADLPYCKKLNGEGNRRIRQFIEQGGAYLGICAGAYYGCAELNFVG
                     RDYSVNGNRELALFNGIAGIAKGSLPQFTNGQLYDKNIGSKAIVEVTLAEGGKMPFYY
                     HGGCCFEPNSTTVIYNPLAYYPDGSVAMIEGELGRGYYLLSGVHFELQAVVYWQKIVN
                     CCENLDIKTQEEAICSNFNVNYGSQIWQHLKQRLNQA"
     gene            complement(12192..12986)
                     /locus_tag="PXD66_000319"
     CDS             complement(12192..12986)
                     /locus_tag="PXD66_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDL6318654.1"
                     /translation="MRFFDTHTHLDYLAESLNLSIPELVENAKQHQVECILIVAVFAK
                     NFAKVTACAKQKPENLVYGLGLHPLYIQQHQQQDLILLENLLSQQDPQCTAIAEIGLE
                     KAVSEVSTPELWKKQCEFLEAQLSLAKKFNLPVNLHSRKSHDQLFVFLKKAQLTKTGV
                     VHGFAGSYEQAKRFVDLGYKIGVGGTITYERANKTREAIRKLPLESLLLETDSPDMPV
                     FGFQGQPNRPERLLVTFNSLCELRTESPNLIAETIWQNSERLFFYA"
     gene            complement(13047..14633)
                     /locus_tag="PXD66_000320"
     CDS             complement(13047..14633)
                     /locus_tag="PXD66_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HDL6318655.1"
                     /translation="MLWFFFCVAVLVLGYFTYGKIIEKIFVINPKRATPAYAMNDGVD
                     YMPMSKTKIWLIQLLNIAGTGPIFGPILGALYGPVAMLWIVIGCIFAGAVHDYFCGML
                     SIRNGGATMPNLAGKFLGRPVKLFINVMALILLLLVGVVFVASPAQLLSTITMDIAGA
                     TQSAMELGDVEAIKESVASGGVTVWGMDKATVISVWTLIIFAYYILATLLPVDKIIGR
                     IYPFFGALLLFMSVGMVYGLVSAHLSATDPIDFFRTVNADGQGLTWEKFTQNFQVKGD
                     VPIWPLLFLTISCGALSGFHATQTPLMARCAQNEKEGRFIFYGAMITEGVIALVWCAV
                     GLSFYENPQALQDAISAGSPSKVVYDSSLHFLGFIGGIFAVLGVVVLPITSGDTAFRA
                     ARLQLAEIFGLEQRTLVKRLYIAVPLFVLGFIVSKVDFSVLWRYFTWANQMTAMVMLW
                     TAAGYLYRYKKFHWVCSIPAWFITTVCATYLANNKIGFGLDYELSVYIGFAITVVCVI
                     CFFAFLKPSEARDEDAYVTN"
     gene            complement(14819..16672)
                     /gene="dxs"
                     /locus_tag="PXD66_000321"
     CDS             complement(14819..16672)
                     /gene="dxs"
                     /locus_tag="PXD66_000321"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDL6318656.1"
                     /translation="MEKKYPLLSEINSPEDLRLLSKDQLQTVVDELRAYLLESVSQTS
                     GHLASGLGVVELTVALHYVYQTPFDQLIWDVGHQAYPHKILTGRRDQMHTIRQKDGIH
                     PFPWREESPYDVLSVGHSSTSISAGLGIAVAAEKENAGRKTVCVIGDGAITAGMAFEA
                     LNHAGALHTDMLVILNDNEMSISENVGALNNHLARIFSGSLYTSVRDGSKKILDKVPT
                     IKNFMKKSEEHMKGVIFSPESTLFEELGFNYIGPIDGHNIDELVKTLSNMRELKGPQF
                     LHIRTKKGKGYTPAENDPIGYHGVPKFDPTAAELPKSKAAPTYSNIFGDWLCEMAERD
                     PKIIGITPAMREGSGMVEFSKRFPEQYFDVAIAEQHAVTFGAGLAIAGYKPVVAIYSS
                     FLQRAYDQLIHDVAIQNLPVIFAIDRAGIVGADGQTHQGAFDLSFMRCIPNMTIMAPS
                     DENEMRKMLYTAYTMNTPSAIRYPRGNAKGVKLEPMQRLEVGKGNIIREGKKVAILNF
                     GALLDEAKIVAEKHNYTLVDMRFIKPLDEALLQKVADSHELLVTLEENAIQGGAGSFV
                     NEYLQNIGKIKPLVMLGIPDFFVPQSTQAEAYADLGLDAQGIEAKIQKFDI"
     gene            complement(16717..17529)
                     /gene="suhB"
                     /locus_tag="PXD66_000322"
     CDS             complement(16717..17529)
                     /gene="suhB"
                     /locus_tag="PXD66_000322"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012621556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HDL6318657.1"
                     /translation="MNPMLNIAIRAARKAGNIIAKGYEQSPQDTQVLQKNTNDYVTEI
                     DKAAEAAIIDVIRKSYPEHSIVTEESGVLNGSNENVQWVIDPLDGTTNFVKRLPHFAV
                     SIAVRENGRTTVGVVYDPIRNELFTAVRGEGAKLNEFRLRIDAERRDLKGTVIATGFP
                     FKVIRHRDAHLNMLEGLMNNGVADFRRTGSAALDLAYVAAGRVDGFFEIGLKPWDFAA
                     GELIAREAGAIVTNFVGGTDYLKTGNLVVGNGRVVKEILNSIQPTLTEELKA"
     gene            17710..18744
                     /locus_tag="PXD66_000323"
     CDS             17710..18744
                     /locus_tag="PXD66_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA methyltransferase"
                     /protein_id="HDL6318658.1"
                     /translation="MQFKENNQPRFKHREERRSDECSPRFSDKGKMRGNEGKSDRPHK
                     LHFKRDEAYSRSEQIYPAEGKKASGEGSVQIWVKGGNSSVKEKKNGPLSPRAPEKIKK
                     NRLEEMKVYGENACISLFQQRPESIVRLWATVEGAKKLGDLMSYLAQHKKAYHVIDRE
                     EMERVTGTEHHGDMCLLVKKTASFVLEGYLQVPKKQDCLVLLDGVNNAQNVGGIIRTC
                     AFYGVKGIITENVDSLNSSAAARVAEGGLEFVRALETKNKHIALVQLRQAGYQIVHLT
                     RHKQAPSLTKIKLAEKVVFVLSEIPASDVAYAEDTTIQLSFNNPLNSGLNVAVNAGIL
                     LNQWYQTHIL"
     gene            18754..19638
                     /locus_tag="PXD66_000324"
     CDS             18754..19638
                     /locus_tag="PXD66_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HDL6318659.1"
                     /translation="MNQQKPFEVIAIVGTPRHDSAFETHLAVYNWLKDRDYQILVETE
                     VATQLQLKESYSLDQIGQRAELVIVIGGDGNMLGIARALAKYRVPLIGINRGNLGFLT
                     DIAPQTAFEQLYSCLVKNEYVIEERFLLEAFIERNGKIIAANNALNEIVVHPTQVARI
                     IEFEVYIDGKFAFSQRADGLIVATPTGSTAYSLSAGGPILTPNMNAMALVPMHPHTLS
                     SRPLVVDGDSQISLRFAKYNTPDLEVICDGQVNMHFTPEDRVLVRKSPDKLQLLHLKD
                     YSYFTVLGSKLGWASKLF"
BASE COUNT         5899 a         4023 c         4271 g         5473 t
ORIGIN      
        1 acttttgctg agcaaaaatt acttgaaaat agcggggcag atccatacct aaacgcaaaa
       61 tttgatgcaa atttgctatt gcaagcagtc acaaaacgtt caaaatcggc catttttgct
      121 ttttcggaaa ccctactttc tgaaagcgag cttgcagaat tagcggaaaa attagctcgg
      181 cgtgccaaag gtgaaccaat ggcatatatt ttaggctatc gggaattttg gtcgttgccg
      241 ttaaaggttt caccggcaac gctgattcct cgccctgata cggaacgact ggttgagttg
      301 gcgttggaat atgctaacaa gcggttagga aatcagaaaa tgttgcaaat tctggatctc
      361 ggtaccggca cgggggcgat tgcgttggca ctggcgagtg aattaggcga gaaagcccaa
      421 attatcgggg tggatttcca aaatgaggcg gtgcaactgg cagaagaaaa tcgccgaaac
      481 ttaggcttaa atcaagttac atttttacaa agcgattggt tcagttcgct tgcaaatcaa
      541 caatttgatt taattgtctc aaatccgcct tatattgatg aatcggacga aaatttgacg
      601 gtgggagacg tacgttttga acctttaacc gcattagttg ccgataataa ggggttgagc
      661 gatttgcaaa aaatcatcga aaatgcaccg cttcacttaa aaccgcaagg ggcgttaatg
      721 ctggaacacg gctggaaaca aggtaacttg gttcgagatt tatttgattt acacttgtgg
      781 gaacaggtag aaactgctca agattatgcc ggcaatgacc gagtaaccaa agcgatttta
      841 aaataaggca gaaaatggac gatattattg aagaattgtt aaaagagttg gaagatccga
      901 aagtaaccat cagcgaaacc gagctgaaaa agtttcttta tcgtgaaatt ttacgattaa
      961 ctacgctcat tgataagaat gtaagtgagc aacaagtgtt tggtcagatg tctgcgttgg
     1021 tgaaaaaagc tcgctataac gtgaatggcg aaggcgatga agagagaatt aaccaattac
     1081 ttcatttagt ctatcgtgaa tggggctttc attgctacta tgaagactat ttccacaccg
     1141 aaaatttgtt gctaaatcaa gtaattcgca aacggcgtgg aatgccggtt tcacttgggg
     1201 cggtggcgct ttatttatcc gccgtattgg atttgccgct ttacccggtt aattttccga
     1261 ctcagcttgt gttacgggct gaaatacacc agccgaacgg ggcaaccaaa gtgcgtttta
     1321 tcaacccgtg gaacggtgaa tttctcacca tcgaaatgct gaataaatgg ctggaaggag
     1381 aaatgggctt tggcgtagaa gttacccctg atttgttaag acgagcagaa ccacaagagt
     1441 tgctggaaag ggtcgaaact gtgtttaaaa tggcacttac ccgtgaaggc aaatatgaag
     1501 aaaccttacg cttgattgaa taccgcttgg catttagccc ggaagatccg tacgaaatca
     1561 gagaccgagg aatggtactg gcaagtatgg attgctacca agcagcatta gaagatatta
     1621 attatttcat tgaccaatgc cctgaagatc cttctgccga aattttaaaa atggaagtga
     1681 aaggactgca acaaaaaagc caagaaagtg tggtgcatta agctgagaag tagccccaaa
     1741 ggggctgagc tatgattaac ctagtacggc tctgccgtgc taggcagaaa ataagaaaca
     1801 aggactccct atgacagata aaattgtaaa agttggcaat attgaagtgg ctaatcacaa
     1861 accgtttacg ctttttggtg gtatgaatgt gctggaaagc cgtgatatgg cacttagagt
     1921 atgtgagcaa tatgttgaag tcacgcaaaa actaaatgtg ccttatgtgt tcaaagcctc
     1981 atttgacaaa gcaaaccgtt cttcaatcca ctcttaccgt ggaccgggaa tggaagaagg
     2041 cttaaagatt ttccaagaat taaaacaaac ctttggcgtg aacatcatta ccgatgttca
     2101 cgaaatttac caatgtaaac ctgtggcaga agtagttgat gtgattcaac taccagcatt
     2161 tttggctcgc caaaccgatt tagtggaagc aatggctcgt accggtgcgg ttattaacgt
     2221 taaaaagccg caatttttaa gcccgggtca aatggggaat atcgtagaaa agattgctga
     2281 atgcggcaac gatcaagtga ttttgtgcga ccgtggcact aactttggct acgataatct
     2341 agttgtggat atgcttggtt tcggcattat gaaaaaagtg tcgaaaggtt gcccggtgat
     2401 ttttgatgtg actcactcat tacaatgccg tgatccattc ggtgcggctt ccggtggtcg
     2461 cagagatcaa gtaaccgaac tggcacgctc cggtatggcg attggtttag ccggcttgtt
     2521 cttagaagct cacccggatc caaactcggc gaaatgtgat ggtccatctg cattaccgct
     2581 gtcaaaatta gaagcctttg tttcacagat gaaagcgatt gatgagttgg ttaagagctt
     2641 tgaagaaatt gatacttcca aataaacttt ctccgcatta aatcctttcc tactttcttt
     2701 gcttcgccaa agaaagtagg caaagaaagg cgaccctact tcgctacttt tcttcgctta
     2761 gtgtgaatgg gcttaacgaa aaatttacat tcgcttcgct cagagtaaat tttcctaccc
     2821 attaaaacac gctcaggtag ctcagaaggg agttagaaag gtaaaagaca gaataggaga
     2881 aaaaaatgct aaatattgtt tttcttgacc gtaccggtat tcctgccact cacgatattc
     2941 ctcgtccgaa ttttccacat acttggacgg aatatgccac cacctctgcc gatcaaactt
     3001 ttgagcgggt aaaagaagcg gatattatca ttaccagcaa agtgttactc cagcgtgact
     3061 tgctggctaa attaccaaag ctgaaattaa ttgcgatcac agcaaccggc acgaacaatg
     3121 tggacttagc ggcagcaaaa gagttaggga ttgcagtgaa aaatgtaacc ggctattcca
     3181 gcgttaccgt gccggaacac gtcattggta tgatttactc cctgaaacat cgtttagccg
     3241 attatcaccg tgatttaatt acgtctgacc gctgggcaac ttgcggccaa ttttgctatg
     3301 tagattaccc gatccacgat gtgcgaggct caaccttagg cattttcggt aaagggaaca
     3361 tcggggctga agtggcacgg ctggcacaag cggtcggaat gcaggttatt tttgcagaac
     3421 atcaaggcgc aactgaaatt cgagaaggct acaccgcctt tgaagaggta ttcaaacaag
     3481 cagatattat ttcactacac tgccctttaa ccgagcagac tcaaaacctg attaatgcag
     3541 aaaccttagc cttaatgaag ccgaccgctt atattatcaa tacgggtcga ggcccactga
     3601 ttgatgaaca ggcattgctt gaggcattag agcaaggcaa gattgccggc gctgcgttag
     3661 atgttttagt gaaagagccg cctgaaaaag acaacccttt aatgttagcc gcaaaacgtt
     3721 taccgaattt actgattacc ccacacgttg cgtgggcaag cgattctgcg gttacaacac
     3781 tagtgaataa agtgacacaa aatatggaag agtttgtagc aacgggtatg tagcttgaag
     3841 taggggctgg tcctgtgcca gccctaatat taatagaatg agagggcggg tataaaaccc
     3901 gcccctacca atatcatttt agaaaaacag atgaatttaa cccctttttt tattgctttt
     3961 cgctatttta gatcgaaaag tgctgaccgt ttcgggcggt tagtcaccaa tcttgccagc
     4021 cttggcattg tgctgggcgt aatggcattg gtgattgtgc tgtcggttat gaacggtttg
     4081 gaaaatatgc agaaaaccaa tctgctatcc accttgccgc acgctatcat cacccctcaa
     4141 gaggggcatt ttgccaaagg tgaaaaactg gctctacctg aatttgccaa gcaaagtgtg
     4201 gtgattaacc gtgcgaatgt gattatccaa agttcacaag gcattaacgc cggtcagtta
     4261 atcggcgtgg aaaatgccac agacgatccg ttgttagtgg cggaaaatat ccccgcatta
     4321 ttgcctagcg gtgaatttaa ggtagtaatt ggctctcgtt tagccaataa attgaacgtt
     4381 acggtaggcg ataagctccg cttaatgatc actgaaaaca gccaatatac gccacttggg
     4441 cgagtgccgg tacagcgttt gtttgaaatt tcgggcgttt atcaatctaa tcgtgaagcg
     4501 agcgaattta ccctctttgc taatttagct gatgtcgggc gtttgcttcg catcagcgaa
     4561 gatcaagtac aaggcgtgcg tttatatttg caagatcctt tccaagttac cgaacttagc
     4621 caatatttcg atgcagaacg ctaccaaatc agtgattggc gtgagcaaaa aggcgaattt
     4681 ttccaagcgg tgaaaatgga aaaaaatatg atgggcttac tcatcagcct gattattgtg
     4741 gttgcaattt ccaacattgt gacttcactc agtttgatgg tggtagacaa acagggcgaa
     4801 attgcaattt tacaaaccca aggcttaacc aaaaaacaag tgatgcagat ttttgtgttc
     4861 caaggggcga ttgtcggagt aattggctcg attattggtg gtttgctcgg cgtattgatc
     4921 gcctacaatt tagatcagat tatcctgcta ttaaacccga gcatccatct gcctacccta
     4981 atttcaggca cgcaaattgc ggtcattgta ggggtgtcga tgttgctttc actcctttgc
     5041 acgctttacc cggcataccg agcctcaaaa attgagccgg cacaggcatt gagatatgaa
     5101 taataagaac ggaaataaaa tgacagaatt acttcgctgt gaacatatca gcaaatttta
     5161 cgatgagggc gaacagaaag ttcaggtgct aaaagaggtg tcattttcga tgaacgaagg
     5221 tgagctggta gcgattgtcg gtagttcagg ttccggcaaa agcaccctat tgcacacatt
     5281 aggcgggcta gatcagccaa gcaccggtga agtctggatt cgagggcaat ctctgcaaca
     5341 gctcagttca gacaaattag cgttattgcg taaccaaaac ttaggctttg tgtaccaatt
     5401 tcatcactta atggcagact ttaccgccct tgaaaatgtg atgatgccga tgctgatcgg
     5461 caaacagaac aaaactgaag ctgccaatcg tgccgagaaa atgctacaag cggtcggatt
     5521 agctcatcga atgacacatc gcccatcagc actttcaggc ggcgaacgcc aacgggtggc
     5581 gattgcccga gcgttggtta ataatccggc tctggtactt gccgatgaac cgaccggtaa
     5641 tttagaccaa aaaaccactg aaagcatttt tgagctgatt caacagctca accaagagca
     5701 gaacattgcg tttttattag taacgcatga tttaaatcta gcgaataaac tttcacgtag
     5761 tcttgtaatg cgagatggcg tgttaagcga ggggagctta tgagtacgcc gtttttcatt
     5821 agctggcgtt accaacgggg caaacagaaa aacaaactgg tttcactgat tgctctcttt
     5881 tccaacatcg gtattgccct tggggttgcg gtattaatta tcgggctaag tgcaatgaac
     5941 ggctttgagc gagagctgaa taatcgtgtg ctttcggttg tgccacacgc tgaactggtt
     6001 tcgtttcaag gcaatcagcc tcaaccaatt tttgatagca aaaaattaga ggcattgata
     6061 aaaaaatcgc aaaatgtgac cgctagttcg ccttttgtca gctttaccgc cttaattgaa
     6121 aatggggctc agcttaaaat cgcccaggtg cgaggggttg atcctaaaaa gcaagatcag
     6181 gccagccaat taagccaatt tattccaccc gagcaatggc aagcgttcaa tcagcaattt
     6241 caagccgatg aagatggctt gattttagga gccggtattg ccaaagcgtt agaagttgag
     6301 gcaggagacg aagttacgct tttaattcct cagccaacag aagacggcaa attagcccag
     6361 cccttgcgtt ttaacctaaa cgtaaccggc attttgcggc tagatgggca gctggatcac
     6421 agttatgcct taattccact gaataaagct caagagctga tggaatacca agcgaatgaa
     6481 gtgtcagggc ttgaaatcag ccttgccaat ccgtttgatg tacaacagct tgaaatgcca
     6541 caacttaacg gctataccca accgctttat ttgaatacgt ggattgataa attcggttat
     6601 atgtataacg atattcaact gattcgcacc gtgatgtaca ttgcaatggt tctcgtgatt
     6661 ggcgttgcct gttttaatat tatttccacc ttgattatgg cagtgaaaga caaacaaggc
     6721 gatatcgcga ttttacggac actgggggca aataacggtt ttatccgccg cattttctta
     6781 tggtatggct taatttcagg tatgaaaggg gcgttattcg ggctgatttt aggtgtaatt
     6841 ttatcgctaa atttaaccgc tattatcaaa gcgattgaag cattctttga ggtgaaattg
     6901 ctttcagacg gggtttattt tgttgatttc ttaccaagtg aattacattg gcaagatgtc
     6961 gtctatgtat tggcagccac gattatccta agcctttttg ccagcttata ccctgccaac
     7021 cgagccgcta aattagaacc ggcaaaagtg ttaagtgggc attaatcatt ttcctattct
     7081 aataaaaaag cagctacccc tcgattatgt cagaaaggta gctgctttta tttatgttat
     7141 tactcttcgc ctaataattt caactcacga gccttaattt ggttttttag aatttcaact
     7201 aagtctgaaa cgaggtaagt acctaagtct gcacctttac gggtacgcac agacacttta
     7261 ccttcggcga tttctttatc accgcaaacc aacatataag gcacacggcg taaggtgtgc
     7321 tcacgcactt tgaagccgac tttttcgttg cgtaagtctg ctttcacacg aatgcctgca
     7381 tcagaaagct gtttcaccac ttgttgcaca taatctgctt ggctgtcggt aatgttcatt
     7441 accactgctt gggttggggc aagccaagtt gggaagaaac cggcatattc ttcggtgata
     7501 atcccgatga aacgttcgat cgagcctaaa atcgcacggt gaatcattac cggtgtgcgg
     7561 cgaccattat cttctgctac ataagaggct gctaaacgac ccggtagagc gaagtcaagt
     7621 tgcactgtgc cacattgcca ttcacgtcct aagcaatcac gcaatgcaaa ctcaattttc
     7681 ggcccgtaga atgcaccttc gccttcttga atttcatatt caagtccgtt gttgcgtaag
     7741 gcgttggcta agcctttttc tgctctgtcc cacattgcat catcaccgat acggttttcc
     7801 ggacgggttg aaagttttac tgcaatgtta gtaaaaccga aggtgctgta aatgtcgtac
     7861 accattttga tacagctgat tacttctgac tcgatttgtt cttcagtaca gaaaatatgg
     7921 gcatcatctt gggtaaaacc acgcacacgc attaagccgt gtaatgaacc ggacggctcg
     7981 ttacggtgac acgaaccaaa ttccgccata cggatcggta aatcacggta agatttcaag
     8041 ccttgattaa agatttgaac gtgacccggg cagttcatcg gcttaattgc atattcacgg
     8101 ttttccgatt gggtagtgaa cattaaatct gcgtagtttt gccagtgtcc ggttttttcc
     8161 caaagcacac gatccatcat aaacggacct tttacttctt ggtaatcgta ctctttcaat
     8221 ttggtacgca caaaagtttc aagttcacgg aaaatcgtcc agccgtcatt gtgccagaac
     8281 accatacccg gtgcttcttc ttgcatatgg tataaatcta acgctttacc gatacggcgg
     8341 tggtcacgtt ttgccgcttc ttccaaacgt tgtaaatatt cggctaattg ttttttatca
     8401 gcccaagctg taccgtaaat acgttgtagc attttatttt tgctgtcgcc acgccagtaa
     8461 gcacccgcta ctttttgtaa tttgaagtgg tggcagaaac gcatatttgg tacgtgtgga
     8521 ccacggcaca tatcaatata ttcttcgtgg tgatataacg caggcgttgc cgtacgttca
     8581 atattttcat ctaaaatcgc cattttgtat ggctcaccac gtttctcaaa ggtatctcga
     8641 gcttcttgcc agcttaccgg gcgttttacc acgtcatagt tggtttttgc cagctctaac
     8701 atacgctttt caagagcgtc taaatcttct tgagtcaaag agcggtctaa atcgatatcg
     8761 tagtagaaac cgttttcgat agtcgggccg atagccattt taacgtctgg gaataattgc
     8821 ttgatggagt gacctaacaa gtgagcggtt gagtggcgaa tgatctctaa accatcttcg
     8881 tctttggcag taatgatttc aagtgtgctg tcttggctga tgatatcaga cgcatcacgg
     8941 cgttcaccgt ttacacgacc ggcaatggtg gctttcgcta aacctgcccc gatagattgg
     9001 gcaacttcca ttacagaaac tgggttgtca aatgaacgtt gtgaaccgtc cggtaaagta
     9061 ataattggca taataaatcc ttacagtggt aatccatacg agagattact tgtgttgttt
     9121 gaatgaaaaa accgcctaca agcggtcttt tttgattaaa attttgcaat ttgcttatta
     9181 tttcccttac aagtggggaa taaaagcact gaaattctag cataaaacaa agaaaaatta
     9241 aactttgctg ttttcagcgg ccccttcatc aagctcaata ttttcatttt ctgtttgtgg
     9301 atcttgttca agtatttcaa gctgagcctg gtactcttgt accgcttctt catcaaatgg
     9361 aataagtgcg tcctcaacaa cttcaatttc ttcaagtggg cttgcggttg caattggggt
     9421 agcatattgc atctctgctc gttccgcttt cggaattgat ttggtgcgga aagcacaaat
     9481 aaataaacta agtgagaaca gtccaaaaag tgcataaaag ccatattcag cggaaaggct
     9541 catcgcaaaa ccgataagcg gtggagcaac taacgccccc acaccatagc aaaacagtaa
     9601 acccctgctt acttcaaccg tgttcatatt attcggcaat tcatcgttgg cacgggcaat
     9661 gctcaaagca tataagctaa acagactaca gccaaagaaa aaggcgataa tatactgtcc
     9721 ccaggcggtc aaattttctt ggaaaattgc aaataatccg gcaagtgcgg ttaaagtggc
     9781 aatcactgaa caagctaaaa tggcgttacg tctgccaata cgattcgata atatcgccat
     9841 cggcatttgg ctgataaaac caccgatcat cgagatcatc aaataaagcg agatttcact
     9901 taatgaaaag ctaaacttca gcagaaagac tgacgccatt gtaaaaaagc cgtttaccaa
     9961 cattccgcta ataaagctgg ttgctaaggc taacggtgaa atcgcaaata gtttaggaat
    10021 actaatgcgc tctcgagccg gaatatcagg ctgaaccatt ttagttaaag caattgggat
    10081 catcgacatc acaaccaaaa tggtagataa gataaaaatg tttcggctac taagttctaa
    10141 acttaataaa cttacgccta aaataaaggc gagatagaag ataatgttat aagtggcaag
    10201 cactttgcct ctggtatcga cattggtttt ttcgcttaac caactttcga ctaaaagcag
    10261 caagctataa tgacagaatc ccaaaataat tcgtaacacg ccccagatcc acaaagtatc
    10321 aatcataata tgaccgagag cggcgatagc aaaaacagca cagaaaacgc taaatgaacg
    10381 gatatgccca acacttgata caatgcggtg agcaaaaata gtgcttaatg ccgccccaat
    10441 aaagaaagca gcgttaagta aaccgatgat gatgtccgtt atccccattt ctgccagttt
    10501 gatccctgct gaatttagaa ataaaccgta gccgaaaaac agaaaaaaga tacctgaata
    10561 aagggagtaa aatttagtgc tattcatcaa tgctccttca aacattctgc cagtattaaa
    10621 tcattgtacc gatttaatac cttttaggca aaaaagttat tattagcgtt gagaaaataa
    10681 cttttggcta ttttgattaa aaaatcaaat gaaggtgatt ttgaaatgta gtaaaaaata
    10741 gagcaatcag aattgaaaaa gggtttgccg taacaaagcc tttagaaaga ttagagtttc
    10801 gagaagaatt tgtagcttgg gctaaccaaa acacttgcta ggagtgcttt aagcacacca
    10861 cctaaaataa atggatataa gcccgcttgt aatactgtgc cttctggtac aaagaaagtt
    10921 gagccaagcc gaaagcaaag gtgatcacac ttgcaattaa ggcgacaaat aaacttttcc
    10981 aaaaagagtt taatacgcct ttatcgctat aatagcctaa gaaataggcg gttggaatat
    11041 agcctaataa gtatccgcct gttggaccaa agaaatgatg gaaacctgaa gcaccgccgg
    11101 caaagacagg taaaccaatc gccccttcaa aaagataaag taatactgca gcagttgcgg
    11161 ctttacgacc cattgcaaaa ccaaccacag ttaaagcaag tgtttgccct gtaatgggta
    11221 cagggtacat cggcacagaa atttgtgcaa aaatagcaag cagtgttgct ccaataaaag
    11281 ttttcaacca aaatgtagtt atactattgg cttctagcca acgtttagtt tgaattaatt
    11341 gcgtagtgaa cataatcctc tcctttaata tggttaaaat gttctcaacc gattatataa
    11401 gaagatgtct tttagggtaa gagtgaattt aaaatggtaa aaccagttta tatttattgt
    11461 gatgaaggta gcagtgaaat tggtgttcaa tcactgttat tggcagtaaa acagcattta
    11521 cagcgtccgg cttatctcat caatgccaaa caaattattc aaagcaagtt ttccgatggc
    11581 tgtttattaa tcatgcccgg tggagccgat ttaccttact gcaaaaaatt aaacggagaa
    11641 ggcaatcgta gaatccgcca atttattgaa caaggtggag cttatctcgg tatctgtgcc
    11701 ggagcttatt atggctgtgc tgaattgaat tttgtagggc gtgactatag cgtaaatggt
    11761 aatcgagaac tagctttatt taatggcatt gcgggcattg cgaaaggcag tctgccgcag
    11821 tttaccaacg ggcaacttta cgataaaaac attggtagca aagcgatagt tgaagtaaca
    11881 cttgcagagg gtggaaaaat gccgttttat tatcacggtg gttgctgttt tgaaccaaat
    11941 tcaacaacag tgatttacaa cccgttggct tattatcctg atggctcagt agcaatgata
    12001 gagggagagc tagggcgagg ttactatctg ttgtcgggtg tacattttga attacaagcg
    12061 gtcgtttatt ggcaaaaaat tgtaaattgc tgtgaaaatc ttgacataaa aacacaagaa
    12121 gaagcaattt gctctaattt taatgtaaat tatggctcgc aaatttggca acatctaaag
    12181 caacgactaa atcaagcgta aaaaaataac ctttcgctat tctgccaaat ggtttctgca
    12241 atcagatttg gtgattctgt tcttaattca catagactat tgaaagtcac taaaagccgt
    12301 tccggtcggt tcggctggcc ttgaaagcca aacaccggca tatcgggcga gtcggtttcc
    12361 agtagtaaac tttcaagagg tagtttgcgg atagcctcac gggttttgtt agcacgttca
    12421 taggtaatag ttccccccac accaatttta taaccgagat cgacaaaacg ttttgcctgt
    12481 tcatagctgc cggcaaagcc gtgaaccaca ccggttttgg ttagttgggc ttttttgagg
    12541 aacacaaaaa gttgatcgtg gcttttacgt gaatgaagat taacgggtaa attaaatttt
    12601 ttcgcaaggg aaagttgtgc ctctaaaaat tcacattgct ttttccataa ttcaggggta
    12661 gaaacttcac ttaccgcttt ttccaaaccg atttcggcaa tagcggtaca ctgtggatct
    12721 tgttggctta gcaaattttc caataagatt aaatcttgtt gttggtgctg ttgaatatac
    12781 agtggatgca accctaatcc atacaccaaa ttttccggtt tttgtttggc acaagcggtc
    12841 acttttgcaa aattttttgc aaatacggca acgataagaa tgcattcaac ctgatgctgt
    12901 ttggcattct caaccaattc cggaatgctt aaattcaggc tttctgcgag atagtcgagg
    12961 tgagtatgag tgtcaaaaaa tcgcataata aaaccaataa aaacggggac tagattgtcc
    13021 ccatttttag cattttacgc aagcaattag tttgttacat aagcatcttc atcacgagcc
    13081 tcagatggct tgaggaaagc aaagaagcaa ataacacaaa ctaccgtaat cgcaaaaccg
    13141 atataaactg ataattcata atctaaaccg aagccgattt tgttgttcgc aaggtaagtt
    13201 gcacacacgg tagtgataaa ccatgcagga atcgagcata cccagtggaa ttttttatag
    13261 cggtataagt aacctgcggc agtccataac ataaccattg cagtcatttg gtttgcccaa
    13321 gtgaagtaac gccataatac gctgaaatca acttttgaga caatgaaacc aagtacgaat
    13381 aatggtacgg caatatacaa acgttttact aaggtacgtt gttctaaacc aaagatttcc
    13441 gctagttgta aacgagcggc tcggaatgca gtgtcacctg aggtgattgg taatactaca
    13501 acgcctaata cggcaaaaat accaccgata aaccctaaga agtgtaacga gctgtcatat
    13561 accactttag acggtgaacc ggcagagatc gcatcttgta aggcttgtgg gttttcatag
    13621 aatgaaagac ctactgcaca ccatactaat gcaattaccc cttcagtaat cattgcaccg
    13681 tagaaaataa aacgaccttc tttctcattt tgcgcacagc gagccattaa tggggtttgg
    13741 gttgcgtgga aaccggataa tgctccgcaa gaaatagtta agaatagtaa tggccaaatt
    13801 ggaacatcac ctttcacttg gaagttttgg gtgaattttt cccacgttaa gccttgaccg
    13861 tctgcattca ctgtacggaa gaagtcgata ggatcagtcg cacttaagtg agcgctgact
    13921 agaccatata ccatacctac agacataaag agtaataatg ctccgaagaa aggataaatt
    13981 cgaccgatga ttttatcaac aggtagtaat gtagcaagaa tatagtaagc aaaaatgatt
    14041 aacgtccaca cagaaatcac ggttgcttta tccatacccc aaacggttac gccacctgat
    14101 gctacagatt ctttaattgc ttcaacatca cctaattcca ttgcactttg ggttgcacct
    14161 gcaatatcca tggtgatggt agagagtaac tgagcagggc ttgcaacgaa aactacgcct
    14221 actaataaaa gcaagatgag cgccattacg ttgatgaata acttgactgg acgacccaag
    14281 aatttccctg ccagatttgg catcgttgcg ccaccgttgc ggatacttaa cataccacag
    14341 aagtagtcgt gtactgcacc ggcaaaaata caaccaatca caatccaaag cattgcaact
    14401 ggtccgtata aggcaccaag tattggtcca aaaatagggc cggtaccggc aatgtttaat
    14461 aattgaatca gccaaatctt ggttttagac attggcatat agtcaacacc gtcgttcatt
    14521 gcataagccg gcgttgctct tttcgggtta atcacgaaga ttttttcaat gatttttccg
    14581 taagtaaagt agccaagaac aagcacggct acacagaaaa agaaccacaa catagtagaa
    14641 atcctataaa ttgagataaa tgatcatttt tagcagaact gataaaggcg tgccattgta
    14701 agtgaggagt gaggataaag taaaatgaat tgttagattt gtgtgaataa tttactgaaa
    14761 atgtgaggtt catcaaaaaa tccctttgtt ctgcgtcaat caaagggact ttttaaagtt
    14821 aaatatcgaa tttttgaatt ttcgcttcaa tgccttgggc atctaatcca agatcggcat
    14881 aagcttcagc ttgagtagat tggggaacga aaaagtcagg aataccaagc attacaagcg
    14941 gtttgatttt gccgatattt tgcaaatatt cattcacaaa actacctgcg ccaccttgaa
    15001 tagcattttc ttcaagcgta actaacaact cgtgtgaatc cgccactttt tgaagtaatg
    15061 cttcatctaa tggtttaatg aaacgcatat ctaccaacgt gtagttatgt ttttcagcca
    15121 caatctttgc ttcatcaagc aaagcaccaa aatttaaaat cgcaactttt ttaccttcac
    15181 gaataatgtt tcctttacct acttcaagtc tttgcattgg ctcaagtttt acccctttag
    15241 cattgcctcg agggtaacga atagcacttg gcgtattcat cgtgtaagcg gtataaagca
    15301 tttttcgcat ttcattttca tcgctcggtg ccataatggt catattggga atacaacgca
    15361 taaaacttaa atcaaatgca ccttggtgag tttgcccgtc agcgccgaca attccggctc
    15421 ggtcaatcgc aaaaatcacc ggcaggtttt gaatagcaac atcgtgaatt aattggtcat
    15481 aagctcgctg caagaagctg gaataaattg cgactaccgg tttgtaacct gcgattgcta
    15541 agcctgcccc gaaagtcact gcgtgttgtt cggcaatcgc cacatcaaaa tattgttcag
    15601 ggaaacgttt ggaaaattct accattcccg agccttcacg cattgccggc gtaatgccga
    15661 taattttcgg atcacgctct gccatttcac atagccaatc gccaaaaata ttggaataag
    15721 taggtgctgc ttttgatttt ggtaactcgg ctgcggtagg gtcaaatttc ggaacgccat
    15781 ggtagccaat cggatcattt tccgcaggtg tgtagccttt gccttttttc gtgcgaatat
    15841 gcagaaattg agggcctttc agctcacgca tattgctcaa ggttttgact aactcgtcaa
    15901 tattatgtcc atcaatcggg ccgatataat taaagcctaa ctcttcaaac aaagtgcttt
    15961 caggggagaa aatcacccct ttcatatgct cttcgctttt tttcataaag tttttgatgg
    16021 taggcacttt atctaaaatt ttcttactgc cgtcacgcac tgaagtatag agcgaaccag
    16081 aaaaaatgcg ggcaaggtgg ttgtttaatg ccccgacatt ttcagagatt gacatttcat
    16141 tatcatttaa gataaccaac atatcggtat gcaatgcacc tgcgtggttt aatgcttcaa
    16201 atgccattcc ggcagtgatt gcaccatcac cgattacaca gacggtttta cggccggcat
    16261 tttctttttc tgctgccacc gcaatgccaa gtccggcact gatcgaagta gaagaatgcc
    16321 caacgcttaa cacatcgtaa gggctttctt cacgccaagg gaaggggtga ataccatctt
    16381 tttggcggat agtgtgcatt tgatctcggc gcccagtaag gattttatgt gggtaagctt
    16441 gatgaccaac gtcccaaatt aattggtcga aaggcgtttg ataaacataa tgcaaagcta
    16501 ccgtaagctc tactacccct aagccagagg ctaaatgtcc gctagtttga ctgactgatt
    16561 ccaataaata ggctcgcagt tcatcaacta cggtttgaag ctggtcttta gaaagtaaac
    16621 gtaaatcttc cggtgagttg atttcgctta acagaggata cttcttttcc ataatgtttt
    16681 attacccaca accatcaaat aatagatgat attaaattaa gctttgagtt cttcggtcaa
    16741 agtaggctga atgctattta aaatttcttt aaccacacgg ccgttaccaa caaccagatt
    16801 acctgttttc aagtaatctg ttccgccaac aaagttagtc acaattgcac cggcttcacg
    16861 ggcaataagt tcaccggcgg cgaaatccca cggttttaag ccgatttcaa agaaaccatc
    16921 aacacgaccc gctgcaacat aggcaagatc taaagcggca gaaccggtac ggcggaaatc
    16981 agctactccg ttattcatta agccttcaag catatttaaa tgtgcatcac gatgtctgat
    17041 gactttgaaa ggaaaaccgg ttgcaattac agtgcctttt aaatcacggc gttctgcatc
    17101 aatgcgtaaa cggaactcgt ttaatttagc cccttcgcca cgcaccgcag tgaaaagctc
    17161 gttacggata ggatcgtaaa ctacccctac tgttgtacgt ccgttttcac gcacggcaat
    17221 cgacacagca aaatgaggta agcgttttac gaaattggtt gtaccatcta atggatcgat
    17281 cacccattgt acattttcat tgctaccgtt taacacgccg gactcttcgg tcacgatcga
    17341 gtgttcaggg taagatttac ggatcacatc aataattgcc gcttccgctg ctttatcgat
    17401 ttcagtcaca taatcattcg tatttttttg taaaacttgt gtatcttgag gcgattgttc
    17461 atagcctttg gcaataatat tgcctgcttt tcgtgccgca cgaatagcaa tgtttaacat
    17521 tgggttcata tttccaccag attttaaaga acataataaa gcacgaatta taaagaatgt
    17581 agcgaaataa agaaacaaca agcggtctaa tctgtgaaaa attttgcaaa aaaatggctt
    17641 tttctaaccg cttggagttt ggattcgtta taatacctcc cattcaagcc acccatttag
    17701 gtactaaaaa tgcaatttaa agaaaataac caacctcgtt ttaaacacag agaagaacgc
    17761 cgttcagatg agtgttcccc ccgtttttca gataaaggca aaatgcgagg aaatgaaggt
    17821 aaaagtgaca gaccgcataa gctacatttt aagcgtgacg aggcttattc acgctctgag
    17881 cagatttatc cagcagaagg caaaaaagcc agtggtgaag gtagtgtgca aatttgggta
    17941 aaaggtggga atagctcagt taaagagaaa aaaaacggcc cgctttcacc gagagcgcct
    18001 gagaaaatta agaaaaatcg cttggaagag atgaaagtct atggcgaaaa tgcctgtatc
    18061 agtttgttcc aacaacgtcc tgaatctatt gtacgtttat gggcaacagt ggagggggca
    18121 aaaaaattag gcgatttaat gagctatttg gctcagcaca aaaaagccta tcacgttatt
    18181 gaccgtgaag agatggagcg agtcaccggt actgaacatc acggcgatat gtgcttattg
    18241 gtgaaaaaaa cggcttcatt tgtgctagag ggctatttac aagtgccgaa aaaacaagat
    18301 tgcttggtac tgttagatgg tgtaaacaat gctcagaatg tggggggaat catcagaacg
    18361 tgtgcttttt atggtgtgaa aggcattatt actgaaaatg tggatagctt aaactccagt
    18421 gctgcagctc gtgtcgcaga gggtggtttg gaatttgtgc gagcgttaga aactaaaaac
    18481 aagcatattg ctttggtaca actccgccaa gccggttatc aaattgtcca cctcacacgc
    18541 cataagcaag ccccttcttt aaccaaaatt aagctggcgg aaaaggtggt ttttgtatta
    18601 agtgagattc ctgcaagtga tgtggcttac gcagaagaca caacaataca gctttccttt
    18661 aataatccgt taaatagtgg tttaaatgtg gcggtaaacg caggtattct acttaatcaa
    18721 tggtatcaaa cccacattct ttaaggaatt gaaatgaatc agcaaaaacc gtttgaagtg
    18781 attgcgattg ttggcacacc tcgccacgac agtgcatttg aaacacatct tgccgtttat
    18841 aattggttaa aagatcgtga ttatcaaatt ttagtggaaa cagaggttgc cactcagtta
    18901 cagcttaagg aaagctacag tttagatcag attgggcagc gcgcagagtt ggtgattgtg
    18961 ataggtggcg atggtaatat gcttggcatc gcccgagctt tggctaaata ccgagtgcca
    19021 ctgataggca ttaaccgtgg caatctgggt tttttaaccg atatcgcacc tcaaacggct
    19081 tttgaacagc tttatagttg cttagtcaaa aatgagtatg tgattgaaga gcgattcctg
    19141 ttagaggctt tcattgagcg caacggtaaa attattgccg caaataatgc cttgaatgaa
    19201 attgtggttc acccaaccca agtggctcga attattgaat ttgaagttta tattgacgga
    19261 aaattcgctt tttcacaacg tgcagatggc ttgattgtgg caacgcctac aggctcaacc
    19321 gcctattcac tttctgctgg tggcccgatt ttaaccccga atatgaatgc aatggcatta
    19381 gtgccgatgc accctcacac tctttcttct cgtcctttgg tggttgatgg cgatagtcaa
    19441 atttcgcttc gttttgccaa atataatacc cctgatttag aagtgatttg tgacgggcag
    19501 gtgaatatgc actttacgcc tgaagataga gtcttggtgc gtaaaagccc ggataaatta
    19561 cagctgctcc atttaaaaga ttatagctac tttactgtat taggttctaa attaggctgg
    19621 gcaagtaagt tgttttaaac acaagcggtg ttttttctga ttaaat
//
LOCUS       DANLXF010000015        19476 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.015, whole genome shotgun sequence.
ACCESSION   DANLXF010000015 DANLXF010000000
VERSION     DANLXF010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 19476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19476
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.015"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..2599
                     /gene="adhE"
                     /locus_tag="PXD66_000325"
                     /gene_synonym="adhC"
     CDS             <1..2599
                     /gene="adhE"
                     /locus_tag="PXD66_000325"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDL6318660.1"
                     /translation="TQPFDAQAEVNELVEKGLKALDEFRKLNQEQVDFIVAKASVAAL
                     DKHGILAMHAYEETGRGVFEDKATKNLFACEYVVNNMRHLKTAGVISEDDVTGITEIA
                     DPVGVVCGITPTTNPTSTTIFKALIALKTRNPIVFAFHPSAQQSSAHAAQIVRDAAVA
                     AGAPENCIQWITQPSMEGTSALMKHPGIATILATGGNAMVEAAYSCGKPALGVGAGNV
                     PAYVEKTAKLKQAVYDIVMSKSFDNGMICASEQAAIVDKEIYAEFIKEMQSYGVYLVN
                     KKEKALLEKYIFGIDKAKDENCSGAKLNSAVVGKPAAWIAEQAGFKVPEKTNILLAEC
                     AFVGEAEPLTREKLSPVLALLKSDSTEHGLELSEAMVNFHGLGHSAAIHTENKALAKT
                     FGERVKAIRVIWNSPSTFGGIGDVYNSFLPSLTLGCGSYGKNSVGNNVSAVNLINIKR
                     VGRRRNNMQWFKVPSKIYFERDSIQYLKSMHDVHKVMIVTDRSMVDLGFVDRITEQLR
                     QRRNQVMIQFFTDVEPNPSLQTVQRGTELMRSFQPDTIIALGGGSPMDAAKIMWLFYE
                     QPEVDFRDLVQKFMDIRKRAFKFPQLGRKAKFVGIPTTSGTGSEVTPFAVITDGDIKY
                     PLADYSLTPTIAIVDPALVMSVPSHVAADTGLDVLTHATEAYTSILANDYTDGLALQA
                     IKLVFENLEKSVKEFDETAREKMHNASTMAGMAFANAFLGICHSMAHKIGGKFHTIHG
                     RTNAILLPHVIRYNGTRPTKVATWPKYTNYVADVRFQDIARMLGLPAETPEQAVEAYA
                     QAVHDLAVRCGVKMSLREQGVDEQAFLNARRELALHAFEDQCTPANPRLAMIEDMEAI
                     MTKAYYGKE"
     gene            2746..3642
                     /locus_tag="PXD66_000326"
     CDS             2746..3642
                     /locus_tag="PXD66_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HDL6318661.1"
                     /translation="MQITILDGGISRELMRRNAPFHQPEWSAAALYEGPHFVQAVHED
                     FIAHGAEVITTDSYAVVPFHIGEKRFAADGKMLADLAGRLAKQAVKNSGNIATQIAGS
                     LPPMFGSYRADLIQMERFAEVAQPLIDGLSPYVDIWLCETQSAIIEPISIKALLPKDN
                     RPLWVSFTLTDDEPTTEPQLRSGESVKQAVEEMVKLGVQAILFNCCQPEVIGEALSVT
                     QKTLAELKATHIRTGAYANAFAPQPKDATANDGLDEVRQDLTLEAYLGWAKKWTEQGA
                     TIIGGCCGIGVEYIETLAKNLK"
     gene            3652..5061
                     /locus_tag="PXD66_000327"
     CDS             3652..5061
                     /locus_tag="PXD66_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL6318662.1"
                     /translation="MSNKKIGLISLTALVLSSMIGSGIFSLPQNMAAVAGAEAITIGW
                     LITGVGIIFLGLSFFFISRLRPDLDGGIYTYAREGFGELMGFMSAWGYWLCATIGIVG
                     YLTVAFEGLGVFTDNPNTVIFGQGNTLASFIGSSIIVWLVHALIAGGIKEAAFVNLIA
                     TFVKVAPLILFIGLGIWYFDVDLFNSDVKATALNNSVSDQVKSTMLITLWVFTGVEGA
                     SVLSAHAKKRSDVGLATVLGIIIALALYVAITILSLGILPRETIANMANPSMGPLLDA
                     MMGPTGKVIITACLIVSVLASYISWTMYSAEVPYRGSKNGAFPKILDKVNKNGTPINS
                     LWFTGFIVQFCLVLVFVFEQSYNTLLLISTSMILIPYFLIGAYLFKLAIQTNATWYIK
                     LTGFMAALYGLWIVYAAGLEYLLLSVVLYVPGILLFLYSNYKFNGRLTLSTAEKVILI
                     VLLVIFGYAVIELPALLAA"
     gene            5168..6391
                     /locus_tag="PXD66_000328"
     CDS             5168..6391
                     /locus_tag="PXD66_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HDL6318663.1"
                     /translation="MQTYQHKALCRVRTITLFLSLTKDKLQWETALNAAKAEFDLLVP
                     AIQTQGYHIQSIRIVTNSFGEYLDLTNIETAKQDLAYLKQLLTQLNDSGLRIRFAIGE
                     AKTPHELALLPELILNYGDLSNACVNIEPDEFGILDNETILQSAQIIKQIAEITPRGE
                     GNFNFTVNFNCKPFIPYFPASYHSSKLPNSFVIGLETPDLLVAVLKELKASPHNDFYR
                     QCYETMSQALQYHVDEVLEAVKSVQHKLQFAFAGIDSSAAPSKNCASMTEVYELMGVP
                     FFGAAGTVEASSLLTKVFKSIKHVPLVGFSGLMLAVTEDLGLAAGTHKAQFDIRALLT
                     YSAVCGIGLDTVPISNEATVEQIAALMRDTGTMAFRLNKPLTVRLFPIPNKSAGEVTE
                     FESDDLCNCRILAVP"
     gene            complement(6429..7625)
                     /locus_tag="PXD66_000329"
     CDS             complement(6429..7625)
                     /locus_tag="PXD66_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL6318664.1"
                     /translation="MKQVIRFIVFSTLSLFSFSTFAKTIVAIDAGHGGKDPGAIGKNL
                     GILEKSVTLAISKELKALLDADPNFKAVMTRSGDYFIQLPQRTEIARKHRADLLVSIH
                     ADSSPRGDSLKGASVWVLSNSRASDEMGKWLEDHEKQSELLGGAGSVLSSNNERYLNQ
                     TVLDLQFSHSQRAGYELGKSILAKMSNVAALAKSSPQHASLSVLRSPDIPSVLVETGF
                     LSNSTEEAKLASANYRKQVARAIYNGLVAYRAKNAVASSSVSKKSDKEDKQNKKEEKT
                     AKADLTKKPASEKQEKNTQSDKTKLKEKSKKEDKKEDSAKTNKKKEQQLKEKTALEKD
                     QKNQSKKSDSQREIKVSNSGYHIVQQDETVYSIARAYGTTPQKISELNNLKNYQITVG
                     KKLKVK"
     gene            complement(7645..8136)
                     /gene="tsaE"
                     /locus_tag="PXD66_000330"
     CDS             complement(7645..8136)
                     /gene="tsaE"
                     /locus_tag="PXD66_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDL6318665.1"
                     /translation="MTDSLSFYCEDETKLLEFGQSFALELKKYLLQDQAHSLVVYLNG
                     ELGAGKTTLTRSIVRAFGHQGNVKSPTYTLVEEYQLPPFSLYHFDLYRLADPEELEFM
                     GIRDYFKPQTLCLLEWAVKGKGMIPEADFVIQIDYKNDGRQISLLPQNQTAVDILVNF
                     HQK"
     gene            complement(8136..8690)
                     /gene="orn"
                     /locus_tag="PXD66_000331"
     CDS             complement(8136..8690)
                     /gene="orn"
                     /locus_tag="PXD66_000331"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HDL6318666.1"
                     /translation="MAQNEQNLIWIDLEMTGLDPEKERIIEIATIVTDKDLNILAEGP
                     VLAVHQTDDLLEKMSDWCVKTHTANGLIERVKASKLTERAAELQTLDFLKRWVPKGAS
                     PICGNSIAQDKRFLYKYMPDLADYFHYRHLDVSTLKELARRWKPEILDKFSKGNTHLA
                     LDDIRESINELKFYREHFIQLDQK"
     gene            complement(8700..9179)
                     /gene="coaD"
                     /locus_tag="PXD66_000332"
     CDS             complement(8700..9179)
                     /gene="coaD"
                     /locus_tag="PXD66_000332"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDL6318667.1"
                     /translation="MSYNVIYAGTFDPITNGHLDIICKASHLFGKVIVAVAQNPSKQP
                     LFSLEERVELVKQSCTQWQNIEVIGFSGLLADFAKQQNAKALIRGIRGADDIDYEIQL
                     AQLNYKLSGDLETIFFPPSIEWRYLSSTMVREIYRHNGDISQFVPQAVKNALKIAKV"
     gene            complement(9181..10461)
                     /gene="waaA"
                     /locus_tag="PXD66_000333"
     CDS             complement(9181..10461)
                     /gene="waaA"
                     /locus_tag="PXD66_000333"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HDL6318668.1"
                     /translation="MLRLLYICLSYLLQPVVLLLMWYKGRKQPAYRKRLWERYGIYDE
                     SEKPKAKGVVIHAASVGEVIAATLLIKAVSKQYPELPLIVTTVTPTGSGRVQAAFGNS
                     VSHFYLPYDLPDAIERFLNFIDPKLMIVIETELWPNLIRKVNIRKIPFVIANARLSPR
                     SAKRYGWVKGSIKDMLNQISLIMAQDEVSRDRYLALGYAPAKMVNTGNLKFDLEITPQ
                     LHQSVIKTKASLNLTERPIWIAGSTHEGEEKLILDAHKLLLEHYPNLVLILVPRHPER
                     FGLVELLIQKSDLNYVKRTENKTLSQNTQVMLGDTMGEMMLLYGLSDIAFVGGSLVKH
                     GGHNPLEPIAFNIPVVSGLYTYNFPEIFEKLREVKGVIEIESSVEALTESIQLLLKQP
                     QIGQEIAKSGLSVLQENQGALKRHLDLLASYLEK"
     gene            complement(10641..12020)
                     /gene="yegQ"
                     /locus_tag="PXD66_000334"
     CDS             complement(10641..12020)
                     /gene="yegQ"
                     /locus_tag="PXD66_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HDL6318669.1"
                     /translation="MTLKYAKPELLSPAGSLKNMRYAFAYGADAIYAGQPRYSLRVRN
                     NEFNHANLKVAIDEAHALGKKFYVVVNIAPHNSKLKTFIKDLKVVVDMKPDALIMSDP
                     GLIMLVRENFPEMDIHLSVQANAVNWATVKFWRQMGLTRVILSRELSLEEIEEIREQV
                     PDMELEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYKMEEGKIDEVGQI
                     VPKYEPEIEVKNVAPTLGNGEATDKVFLYTESQRPDEQMTAFEDEHGTYFMNSKDLRA
                     VQHVERLTQMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDESLMDTLESL
                     AHRGYTEGFLRRHTHDEYQNYEYGYSISERQQFVGEFTGKRNAEGLAEVAVKNKFLVG
                     DSVEMMTPKGNIVFKIERMLNRKNENVDAALGDGHFVFLDVPADIELDFALLMRNLVN
                     TNTRNPHQA"
     gene            12148..12519
                     /locus_tag="PXD66_000335"
     CDS             12148..12519
                     /locus_tag="PXD66_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318670.1"
                     /translation="MKLKYLLATTLLAFSTFTAAQSIKTEMMQMNMQANKLMRAESAE
                     EFSEIANDFIIITEKTKGIFPNSVEGDKAAQEDYQAGMQKLIDMVNQASEKAQAGELQ
                     EAKMLISELEMIKREYHKKYK"
     gene            complement(12558..16034)
                     /gene="dnaE"
                     /locus_tag="PXD66_000336"
     CDS             complement(12558..16034)
                     /gene="dnaE"
                     /locus_tag="PXD66_000336"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDL6318671.1"
                     /translation="MSDCRFVHLKVHSDFSMINGLAKVKSLVKTAAANNMVAMGLTDF
                     INFCGLVKFYGEALGAGIKPIIGADVYVRTESGNEDYFELTLLAKNNKGYHNITQILS
                     KAYQQGYVEFPMVEREWLAELNEGIIILSGGRMGDVGKALLKENRQEAEEKLAFYQQY
                     YPNHFYLSVCRTGRADEERYIKQAVEFSQKNAIPLVAVNDVVFLKEDDFDAHEIRVAI
                     HDSYTLDDPKRPKKYSPKQYFRTEEEMCKLFADLPSALANTVEIAKRCSVTVRLGEYF
                     LPNFPTGELSTEDFLVKKSKEGLEERLEFLFPDPEERANKRSVYDERLQVELDVINQM
                     GFPGYFLIVMEFIQWSKDNDIPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFLN
                     PERVSMPDFDVDFCMDGRDRVIEHVAETYGRQAVSQIITFGTMAAKAVIRDVGRVLGH
                     PYSFVDRISKLIPPDPGMTLEKAFAAEPKLPELYEADEEVKDLIDMARKLEGVTRNAG
                     KHAGGVVIAPTAITDFSPLYCDSEGLHPVTHFDKNDVEYAGLVKFDFLGLRTLTIIKW
                     ALEMINERLAREGKEPIRIETIPLDDKKSFALLLAAKTTAVFQLESRGMKDLISRLKP
                     DCFEDIIALVALFRPGPLESGMVQNFIDRKHGKEEVSYPDPQYQHECLKPILEPTYGV
                     IVYQEQVMQIAQELAGYTLGGADLLRRAMGKKKPEEMAAQREVFEKGAEAKGIDGKLA
                     GQIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTSEMDNTDKIVG
                     FYDECMNMGLTVVPPDVNSGKHRFSVNEKGEIVYGLGAIKGVGEGPIEAILAARQKEG
                     KFKDLFDLTARVDLKKINRRTFESLIMSGAFDKLGPHRAALMKNLEDALKASDQHAKM
                     EELGQSDMFGVLTETPEEVQNAYANTPKWSEQVILDGERATLGLYLSGHPIGRFLKEL
                     SHYAPNRLNELQPTFRGQMTTVSGIVMASRVAVTKRGSRIGIATIEDRSGKLDITLFS
                     EALENFGHLMQQDQIIVATGSVQFDDFSGGLKMSAREVLSLDEARGRFAKSLALAISK
                     EQLSADFIKKMTAIITPHKEGTLPLHFYYQSPEGRVLLKGGVEWRISPNEAMFSELKA
                     LLGENSVELEFE"
     gene            complement(16259..17356)
                     /gene="lptG"
                     /locus_tag="PXD66_000337"
     CDS             complement(16259..17356)
                     /gene="lptG"
                     /locus_tag="PXD66_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HDL6318672.1"
                     /translation="MQFFSMNILERYIGKTILSAIFLTLFMLIGLGAIIKFVEEFRDV
                     GRGSYDGLKAAYYTFLTIPRDIETFFPIAALLGSLLGLGNLASLSELVVMQASGFSRF
                     RIGLAVMKTALPLVIFTMIIGEWGVPQTEQFARNMRSIAQSGGSMLATKDGFWAKDGN
                     SFIYINHIKNEKSLDNVLIYQFGEGQNKRELQSVLKARTANYSDEKGWVLSKVEKSTV
                     GENQIQQAKQPDQVWQTSLTPSKLGIVSLKPESLSISGLADYVGFLKDTGQDPKRFEI
                     TFWRKVFQPISMAVMMLLAISFIFGPLRSSTMGAKIVIGIVAGFMFYVANIVFGNLSL
                     IATWLPIPVGALIPSLLCLIIVWWLLSKKRD"
     gene            complement(17356..18465)
                     /gene="lptF"
                     /locus_tag="PXD66_000338"
     CDS             complement(17356..18465)
                     /gene="lptF"
                     /locus_tag="PXD66_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HDL6318673.1"
                     /translation="MILSRYLTKEIFKSQLAILFILLLIFFCQQLVRVLSSAVSGKVP
                     ADLVVSLLGLGMPTMAQLMLPLSLFIALLLTLGRFYAESEITVMRACGIGQGLLVKVA
                     MFLSIFTTALAVYNVFWLTPWAINKQSEMLAEAKANPRFAALSAGQFMSAGGYVLFID
                     KIENNSLNDIYVFQPEQQKKNKPSVVVAESGTLQGLPNGDQVLTLAESKRFEGTPQSA
                     DFRISHFENYSAYLGYQDVESDEKLVQRADFKKLMETKTPEAKAELHWRLALILAVPL
                     MALLAVPMSSVNPRQGRFAKLLPALLLYLIYFLLQSSLKSSGASGKLNAEVLMPLVSV
                     AFLVLGVILNAWDSKFMSKLRYQFGAKKLTNKAVA"
     gene            complement(18639..19436)
                     /locus_tag="PXD66_000339"
     CDS             complement(18639..19436)
                     /locus_tag="PXD66_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318674.1"
                     /translation="MRFWGKTFVVGLIGLGTQNTIFANDDAQISRYEQAKLRLSYDGN
                     VGNLLQQLSQRLKVGFIAYDVDITRKISLQNETETAIKTINEQIESQLTNIDVRFEKI
                     GERLFLVVSAKDVAPLLQNEPKEVQFVGDVIFDGEPNLTEQAQSTAEATKVQNILNIA
                     TNQEKIMAAKGKKAPQYKTVTKDNIGLKNIRVTALGTFLVFDTSVDAAKLRVKGNFED
                     IAQGENIIAILHQNTEAPPKIEIEDSQGKKLILEAHKATSSKKSKKQ"
BASE COUNT         5554 a         4438 c         3791 g         5693 t
ORIGIN      
        1 tacgcaacca tttgatgccc aagcagaagt gaatgagctt gttgaaaaag gcttaaaagc
       61 ccttgatgag tttagaaaac tcaaccaaga acaagtggat tttattgtcg ctaaagcctc
      121 tgttgctgcc cttgataaac acggtatttt agcaatgcac gcttacgaag aaaccggacg
      181 gggtgtgttt gaagacaaag ccactaaaaa cctgttcgcc tgtgagtatg ttgtcaataa
      241 tatgcgacat ttgaaaaccg caggggtcat tagtgaagat gatgtcaccg gtattaccga
      301 aattgccgac cctgtcggtg ttgtctgcgg tatcacccca accaccaacc caacctcaac
      361 caccattttc aaagcgttaa tcgcccttaa aacccgtaac cctatcgttt ttgctttcca
      421 cccgtctgct caacaatctt ctgcccacgc tgctcaaatt gtgcgtgatg ctgccgtggc
      481 tgccggtgca ccggaaaact gtattcaatg gattacccag ccttcaatgg aaggtacatc
      541 tgctttaatg aaacacccgg gcattgcaac tattttagcc accggcggta atgcaatggt
      601 tgaagccgct tactcttgcg gcaaaccggc attaggagtt ggtgcaggca acgtaccggc
      661 ttatgtagaa aaaaccgcta aattaaaaca agcggtgtat gacattgtaa tgtcaaaatc
      721 tttcgacaac ggtatgattt gtgcctctga acaagcggcg attgtagata aagaaattta
      781 cgctgagttt attaaagaga tgcaatctta cggcgtctat ttagtgaaca aaaaagagaa
      841 agccttattg gaaaaataca ttttcggtat tgataaagcc aaagatgaaa actgctcagg
      901 tgcaaaactc aattcagcgg tggttggcaa accggctgcg tggattgcag aacaagccgg
      961 ctttaaagta ccggaaaaaa ccaatattct gttagccgaa tgtgcctttg tgggcgaagc
     1021 tgagccactt acccgtgaaa aactttcgcc tgtactggct ctgttaaaat ccgactccac
     1081 cgaacacggt ttggaattgt ctgaagcgat ggtgaatttc cacggtttag ggcatagtgc
     1141 cgccattcat accgaaaaca aagccttagc caaaaccttt ggcgaacggg taaaagccat
     1201 tcgggtgatt tggaactcac catcaacctt tggcggtatc ggtgatgtat ataactcctt
     1261 cctgccatcg cttacgctgg gctgtggttc ctacggtaaa aactctgtag gtaacaacgt
     1321 tagtgcggtg aatttaatca atatcaaacg tgtgggcaga cggagaaata atatgcaatg
     1381 gtttaaagtg ccatcaaaaa tctactttga gcgtgactca attcaatatt tgaaatcaat
     1441 gcacgatgtg cataaagtaa tgatcgtaac cgaccgctca atggttgatc tcggctttgt
     1501 ggatagaatt accgaacaac ttcgccaacg tcgcaaccaa gtgatgattc agttctttac
     1561 cgatgttgaa ccaaacccaa gcctgcaaac cgtacaacgt ggtacagaat taatgcgtag
     1621 cttccagcct gatactatca tcgcacttgg tggtggttca ccaatggatg cagccaaaat
     1681 tatgtggtta ttctacgaac aaccggaagt cgatttccgt gatttagtgc aaaaatttat
     1741 ggatatccgc aaacgtgcat tcaaattccc acaattaggc agaaaagcga aatttgtcgg
     1801 cattccaacc acctccggaa caggctctga agtcacaccg tttgcagtaa ttaccgatgg
     1861 cgatattaaa tatccgctcg ctgactactc gcttacccca accattgcga ttgtcgatcc
     1921 tgcgttagta atgtctgtgc cgtctcacgt tgctgccgac accggtttag acgtattaac
     1981 tcacgctacc gaggcttaca catcgatttt agccaacgac tataccgacg gcttagcctt
     2041 gcaagccatt aagttagtgt ttgaaaactt ggaaaaatcg gtcaaagagt ttgatgaaac
     2101 cgcccgtgaa aaaatgcaca atgcttccac aatggcaggg atggcatttg ccaatgcctt
     2161 cttaggtatt tgccactcaa tggcacacaa aatcgggggt aaattccaca ccatccacgg
     2221 tcgaaccaat gcgattttac tgccacacgt tattcgctat aacggcacac gcccaaccaa
     2281 agtggcaact tggccaaaat acaccaatta tgtcgcagat gtgcgtttcc aagatattgc
     2341 cagaatgctt ggcttaccgg cagaaacccc ggaacaagcg gtggaagctt acgcccaagc
     2401 ggtacacgat ttagccgttc gttgcggcgt gaaaatgtcg cttcgtgagc aaggcgttga
     2461 tgagcaagcc ttcttgaatg cccgccgtga acttgcccta cacgcatttg aagaccaatg
     2521 cacaccagca aacccacgtt tggcaatgat tgaagatatg gaagcgatta tgactaaagc
     2581 ctactatggc aaggaataaa gcacaattta atcgttaaac ttactaaatg gccgcttgca
     2641 agcggtcatt tttcttgaaa tttttacttt tcccttcaca aaatactttc gctcttttac
     2701 aaagcatagt atttttatcg ctccttttat cttacaggaa atactatgca aattacgatt
     2761 ttagacggcg gaattagccg tgaattaatg cgtcgcaatg ctccatttca tcagcctgaa
     2821 tggtctgctg ccgctttgta tgaaggccca cattttgtgc aagcggtaca cgaagatttt
     2881 atcgctcacg gggcagaagt gattactacc gacagctatg cggttgtgcc gtttcacatt
     2941 ggcgaaaaac gctttgcggc agacggcaaa atgcttgccg atttagccgg tcgccttgcc
     3001 aaacaagcgg tcaaaaattc aggaaatatt gcaactcaaa ttgcaggctc acttccacca
     3061 atgtttggct cttaccgtgc cgatttaatt caaatggaac gctttgctga agtcgctcag
     3121 cctttaattg acgggctttc gccttatgtg gatatttggc tttgcgaaac ccaaagtgcg
     3181 attattgagc cgatttccat caaagcatta ttgccgaaag acaaccgccc gctttgggtc
     3241 tcttttaccc tcaccgatga tgaaccaacc accgagccac aacttcgctc gggcgaaagc
     3301 gttaaacaag cagtcgaaga aatggtgaag ttaggcgtgc aagcgatttt atttaactgc
     3361 tgtcagcctg aagtgatcgg cgaggcatta agtgtaaccc aaaaaacttt agccgaatta
     3421 aaagctaccc atattcgcac aggagcttat gccaacgcct ttgctccaca accaaaagat
     3481 gccaccgcca atgacggttt agatgaagtt cgccaagatt taaccctgga ggcttattta
     3541 ggctgggcaa aaaaatggac ggaacaaggg gcaactatta tcggtggctg ctgcggcatt
     3601 ggggttgagt atattgaaac gctggcaaaa aatttaaaat aaggaaatcg aatgtcaaat
     3661 aaaaaaatag gattaatttc actcaccgcc ttagtgctaa gctcaatgat tggctcgggg
     3721 attttcagct taccgcaaaa tatggcagcc gttgccggag cagaagccat taccattggc
     3781 tggctgatta ccggtgtcgg cattatcttt ttaggtttat cgtttttctt tatttcccgc
     3841 ttgcgtccgg atttagacgg tggcatttac acctacgcac gggagggttt tggcgagctg
     3901 atgggcttta tgtcagcgtg gggctattgg ctctgtgcca ccattgggat tgtaggatac
     3961 ctcaccgttg cttttgaagg tttgggtgta tttaccgaca acccgaacac cgtgattttc
     4021 gggcaaggta acaccctcgc ctcttttatt ggatcttcaa tcattgtgtg gctggttcac
     4081 gccctcatcg caggcggtat caaagaagct gcctttgtaa acttaattgc cacttttgtg
     4141 aaagtcgctc cgctcattct gtttatcggg ttaggcattt ggtatttcga tgtggatctg
     4201 tttaatagcg atgttaaagc caccgcactt aataatagtg taagcgacca agtgaaaagc
     4261 acaatgttaa tcaccttatg ggtatttacc ggtgtggaag gggcttctgt gctgtcggct
     4321 cacgcgaaaa agcgttctga cgtgggactg gcaaccgtat taggtattat tattgcactt
     4381 gctctttatg tggcaattac cattctttca ctcggaattc tgccacggga aactattgcc
     4441 aatatggcaa atccgtcaat ggggccgttg ctcgatgcaa tgatgggccc aaccggcaaa
     4501 gtgattatta ctgcttgttt aattgtatcg gtgcttgcct cttacattag ctggacgatg
     4561 tattcggcag aagtgcctta tcgtggctca aaaaacgggg ctttcccgaa aattttagac
     4621 aaagtgaata aaaacggcac gccgattaac tcactctggt ttaccggctt tatcgtgcaa
     4681 ttttgcttgg tgttggtgtt tgtttttgag caaagctata acaccttatt gctgatttcc
     4741 acctcgatga ttttaattcc ctatttctta atcggggctt atttattcaa attagcgatt
     4801 caaaccaacg ccacttggta cattaaactc accggcttta tggcagccct ttacggatta
     4861 tggattgtct atgccgcagg actagaatat ttgctgcttt cggtggtgct ttatgtaccc
     4921 ggaattttac tcttcctcta ctccaactat aaattcaacg gcagattaac cctttcaacc
     4981 gctgaaaagg tcatcttaat cgtgctactg gtaatttttg gctatgccgt gattgagctg
     5041 ccagcactat tagcagcata attcccatca aaaatgaccg cttgcaagcg gttatttttc
     5101 taggatcttc tgcaaagatt cattagaata gaaacaacgt caataataaa taaaaagagg
     5161 agaaacaatg caaacttatc aacataaagc cctttgtcgg gtaagaacca ttaccctttt
     5221 tctgagttta accaaagata aattgcagtg ggaaacggca ttaaatgcgg caaaagcaga
     5281 atttgatcta ttagttcccg ctattcaaac acaaggctat cacatacaat cgattcgaat
     5341 cgttaccaac agtttcgggg aatatcttga tctcactaat attgaaacag cgaaacagga
     5401 tttagcctat cttaaacagc tattaaccca acttaacgat agcggtttac gcattcgctt
     5461 tgccattggt gaggcaaaaa caccccacga attagcatta ctacctgagc tgattttaaa
     5521 ttatggtgat ttatctaacg cttgtgtcaa tattgaaccc gatgaatttg ggattttaga
     5581 caatgaaacc attttacaat ctgctcaaat tatcaaacaa atcgctgaaa ttaccccaag
     5641 aggtgaaggc aattttaatt ttacggtgaa ttttaactgt aaacctttta ttccttattt
     5701 tccggcaagc taccattcaa gcaagctccc taacagtttt gtgataggcc tagaaacacc
     5761 agatctatta gtagcggtgt taaaagagct gaaagccagc ccacataacg acttttatcg
     5821 ccaatgctat gaaacgatgt ctcaagcctt gcaatatcac gttgatgaag ttctcgaagc
     5881 agtaaaatcg gtacaacaca aattacaatt tgcctttgct ggcattgata gttctgctgc
     5941 tccgtcaaaa aactgtgcct caatgacaga agtttatgaa ttaatgggcg taccattttt
     6001 tggggcagcc ggaacagtag aagcctcttc acttctgaca aaagtcttta aatctatcaa
     6061 acatgtaccg cttgtcggtt tttcaggttt aatgttggca gtaaccgaag atttaggctt
     6121 agccgcaggc acacacaaag cccaattcga cattcgtgct ttactaactt atagtgctgt
     6181 ttgtggcatt gggttagata ccgtaccgat ttccaatgaa gccactgttg aacaaattgc
     6241 cgcattaatg cgagacaccg gcacaatggc atttagacta aataaaccgc ttacagtacg
     6301 tttatttcca attccgaata agagtgctgg ggaagtgaca gaatttgaaa gtgatgattt
     6361 atgtaactgc cgaattttag ctgtacctta aaataaaggc tgatggatat ttccatcagc
     6421 ctttcaattt acttcacctt caatttttta cctaccgtga tctgataatt tttcaaatta
     6481 ttcagctcac tgattttttg cggcgtagta ccgtaagctc tagcaatcga gtaaaccgtt
     6541 tcatcttgtt ggacaatatg gtagccactg ttagacacct taatttctct ttgagagtct
     6601 gattttttag actgattttt ttgatctttc tcaagagctg ttttttcttt tagttgctgc
     6661 tcttttttct tattggtttt agccgaatct tcttttttat cctctttttt gctcttttct
     6721 ttcaattttg tcttatctga ttgcgtattc ttctcttgtt tctcagaagc aggcttttta
     6781 gttaaatcag ctttagcagt tttttcctct ttcttatttt gcttatcttc tttgtccgat
     6841 tttttactga ccgaagaact agcaactgca ttttttgccc gataagcgac taatccatta
     6901 taaatggccc gagcaacttg tttacgataa tttgcactgg ctagctttgc ttcctcggtt
     6961 gaattagata aaaatcccgt ttccaccaat acagatggaa tatcaggtga acgcagaaca
     7021 cttaagcttg cgtgctgagg agacgatttt gctaatgcag ccacatttga cattttagct
     7081 aaaatgctct tgcctaattc atagccggca cgttgagagt gagagaactg taaatcaagc
     7141 actgtttgat ttaaataacg ttcattgttg cttgaaagca ccgaaccagc cccgccaagt
     7201 aattccgact gcttctcgtg gtcttccaac catttaccca tttcatcgct agcacggcta
     7261 ttagaaagta cccaaaccga agcccctttc aaggaatcgc cacgaggcga agagtctgcg
     7321 tgaatagaaa ccagcagatc ggcacgatgc ttacgggcaa tctcggtacg ttgcggtaat
     7381 tggataaaat aatctcccga gcgtgtcatc actgctttaa aattaggatc ggcatctaac
     7441 aaggctttta gctctttaga aattgcgagg gtaacgcttt tttccaaaat ccccagattt
     7501 ttaccaatag cccccggatc ttttcccccg tgtccggcat caatcgccac aatggttttg
     7561 gcaaatgtgg aaaaactgaa taaactcaat gtgctaaaaa caataaatct gataacttgc
     7621 ttcattacct tccctacaag cggtctattt ttgatgaaaa tttactaata tatctactgc
     7681 agtttgattt tgcggcaaca ggctaatctg ccgtccgtca ttcttataat caatttggat
     7741 cacaaaatca gcctctggaa tcattccttt acctttaact gcccattcaa gcaaacaaag
     7801 cgtttgtggt ttgaaataat cacgaattcc cataaactcc agctcttcag gatcggctaa
     7861 gcgatacaaa tcaaaatggt aaaggctgaa aggcggaagt tggtattctt ccaccagagt
     7921 ataagttggg ctttttacat tgccttgatg cccgaaagcc cgtacgatcg agcgggttaa
     7981 ggtggtttta cctgccccaa gttcgccgtt taggtaaaca accagcgaat gagcttggtc
     8041 ttgcaataaa tatttcttaa gttctaacgc gaatgattga ccaaattcca gcaatttagt
     8101 ttcatcttcg cagtaaaaag agagagaatc tgtcattatt tttggtctag ctgaataaaa
     8161 tgttcccgat aaaatttcaa ttcgttaatg gattcacgaa tatcgtccaa cgctaaatgc
     8221 gtattgcctt tactgaattt atctaaaatc tccggtttcc aacggcgtgc cagctctttt
     8281 aaagtactca catctaagtg gcgataatgg aaataatcgg ctaaatcagg catatattta
     8341 tacaaaaaac gtttatcttg agcaatgctg ttgccgcaaa tcggagaagc gccttttggc
     8401 acccagcgtt ttaagaagtc taaagtttgc aactctgctg cccgttcagt caatttgctc
     8461 gctttcactc gttcaatcag cccgttagcg gtatgggttt tgacgcacca atcactcatt
     8521 ttctctaata aatcatcggt ttggtgtacc gcaagtaccg gtccttccgc taaaatattt
     8581 aaatccttat cggtcacaat ggtggcgatt tcaataatgc gttctttttc cggatctaac
     8641 cctgtcattt caagatcgat ccaaattaaa ttttgttcgt tttgagccat tttatttcct
     8701 tacacttttg caatttttag ggcatttttg accgcttgcg gcacaaattg gctgatgtca
     8761 ccattgtggc gatagatttc ccttaccatc gttgaagaaa ggtaacgcca ttctattgaa
     8821 gggggaaaaa aaatagtttc taaatcgccc gataatttgt aatttaactg agcgagttgg
     8881 atttcatagt caatatcatc tgccccacga ataccacgga ttaacgcttt ggcattctgc
     8941 tgtttggcaa agtctgctaa caatccacta aagccaatca cctcaatatt ttgccactgg
     9001 gtacagcttt gtttaacaag ctccactcgt tcttcaagac taaaaagcgg ctgcttgctt
     9061 gggttttgag caacggcaac aatcactttt ccaaataagt gagaggcttt gcaaattatg
     9121 tccaaatgcc cattggtaat gggatcaaag gtgcctgcat aaattacgtt ataactcatt
     9181 ttatttctct aaataggagg ctaataaatc caaatggcgt tttaatgccc cttgattttc
     9241 ctgtaacacg cttaagccgg attttgcaat ttcttgtccg atttggggct gcttaagcaa
     9301 taattgaatg ctctcggtca aagcctcgac tgaactttca atttcaatca cgcctttcac
     9361 ctcacgcaat ttctcaaaga tttcagggaa attataggtg taaagcccag aaaccaccgg
     9421 aatattaaaa gcgattggtt caagtggatt atgcccaccg tgtttgacca aactgcctcc
     9481 tacaaaagca atgtcagaca acccatacag taacatcatt tcccccattg tatcacctaa
     9541 cattacttga gtattttggc ttaacgtttt attctcagta cgttttacat aatttaaatc
     9601 tgatttttga atcagtagct caactaaacc aaaacgctcc ggatggcgag gcactaaaat
     9661 caacactaaa ttcgggtaat gctctaataa gagtttatga gcatctaaaa tcagcttctc
     9721 ttccccttcg tgagtactgc cggcaatcca aatcggtctc tctgttaaat tcagactagc
     9781 tttagtttta atcaccgatt gatgaagctg tggcgtaatt tctaaatcaa atttcaggtt
     9841 accggtatta accatttttg ccggtgcata accgagtgct aaatagcgat ctcggcttac
     9901 ctcatcttga gccataatca gactaatttg gttgagcata tccttaatgc taccttttac
     9961 ccagccataa cgcttcgcag aacgtggcga taagcgggca ttggcaatca caaatggaat
    10021 tttacgaata tttactttac gaattaaatt cggccaaagt tcggtttcaa ttacaatcat
    10081 caatttcgga tcgataaaat tcaaaaaccg ctcgatagca tcaggtaagt cataaggcaa
    10141 ataaaaatgt gaaacactat taccaaaagc agcttgcaca cgcccagagc ctgttggcgt
    10201 taccgttgtg actatcaacg gaagttcagg atattgcttg ctgactgcct taatcaaaag
    10261 cgttgcagca atcacctcac caaccgatgc cgcatggatt accacccctt ttgctttcgg
    10321 tttttccgat tcgtcataaa tcccataacg ctcccataaa cgtttccgat aagccggttg
    10381 tttacgccct ttgtaccaca ttaatagcag aaccacaggc tgtaatagat aacttaaaca
    10441 aatatagagt aaacgtagca tttattttcc ttataccata accctcacct cttgtgggag
    10501 agagtcaaat acgccaataa atcgttcctc tcttgttcga gaggggggaa caagcggtta
    10561 tttttaccaa aaattttgca atctatatcc tcaaagacaa aaccacggaa taaccttaat
    10621 ccgtggttta tctcgcatca ttacgcttga tgtggattgc gggtattcgt attcactaag
    10681 ttacgcatta aaagtgcaaa atccaactca atatctgccg gcacatctaa aaagacaaaa
    10741 tgcccgtcac cgagtgctgc gtccacattt tcatttttgc ggttaagcat acgctcgatt
    10801 ttaaatacaa tgttgccttt aggggtcatc atttccactg aatcgcccac taagaatttg
    10861 tttttcactg ccacttccgc caagccttct gcattgcgtt tgccggtaaa ttcgcccaca
    10921 aattgttggc gttctgaaat cgaatagcca tattcatagt tttggtattc atcgtgagta
    10981 tgacgacgta agaagccctc tgtataacca cggtgtgcaa ggctttcaag agtatccatt
    11041 aaactttcgt caaacggttt accggcagcg gcatcatcaa tcgccttacg atacacctga
    11101 gccgttctcg cacagtaata gaacgatttc gtacgcccct caattttcag tgaatgcacc
    11161 cccatttgag ttaaacgctc aacgtgttgt actgcacgca gatctttaga gttcataaaa
    11221 taggtgccgt gttcatcttc aaaggcggtc atttgctcat cagggcgttg tgattctgta
    11281 taaaggaaca ctttatctgt tgcttcgcca ttacctagag taggagccac atttttgact
    11341 tcaatttccg gctcatactt aggcacaatt tgccctacct catcaatctt accttcttcc
    11401 attttatatt cccaacggca agcattggtg caagtgcctt ggtttgggtc acgcttattg
    11461 atatagcctg aaagcaagca gcgacctgaa tatgccatac acaaagcacc gtgaacgaag
    11521 atctcaagct ccatatccgg cacttgttca cgaatttctt cgatctcttc gagtgataat
    11581 tcacgggata aaatcacacg ggttagcccc atttgacgcc agaatttcac cgttgcccag
    11641 tttaccgcat tggcttgcac cgaaagatga atatccattt cagggaaatt ttcacgcacc
    11701 aacataatta agcccggatc agacataatc aaagcgtccg gcttcatatc cactaccact
    11761 tttagatctt tgatgaaggt ttttaatttt gagttgtgtg gcgcaatgtt taccaccaca
    11821 tagaattttt ttcctaaggc gtgggcttca tcaatcgcaa cttttaaatt ggcgtggtta
    11881 aattcattat tacgcacacg caagctataa cggggctgtc ctgcataaat cgcatcggca
    11941 ccataagcaa aagcgtaacg catatttttt aaagaacctg caggcgaaag tagctcaggt
    12001 ttggcatatt ttaaagtcat attattctct tttctgataa caagtcagga cttctcgacg
    12061 tgagaagcgt aagcggtata ttttctaaga aatattgcgt gatggcaagc taatccatta
    12121 caatccgatt tttatgtggg gaacaatatg aaacttaaat accttttagc aacaacatta
    12181 ctggcttttt caacatttac cgccgctcaa agcatcaaga cggagatgat gcaaatgaat
    12241 atgcaagcga ataaattgat gcgtgccgaa tcggcagaag agtttagcga aattgcgaat
    12301 gatttcatta taattaccga gaaaaccaaa gggatttttc ccaatagcgt agaaggcgat
    12361 aaagcagctc aggaagatta ccaagccggt atgcaaaaat taattgatat ggttaatcaa
    12421 gccagtgaga aagctcaagc aggtgaattg caagaagcaa aaatgctgat ttccgagctt
    12481 gaaatgatca agcgtgaata tcacaaaaaa tacaaataat tcaacacatt aaaagggtga
    12541 gctaaagctc accctttcta ttcaaattcc agttcaaccg aattctcacc aagaagtgcc
    12601 tttaattcgc taaacattgc ctcattcggg ctgattcgcc actctactcc gcctttgagc
    12661 aataccctac cttccgggct ttggtaataa aaatgcagcg gtaaagtgcc ttctttatgt
    12721 ggggtgatga tagcggtcat ttttttaata aaatctgcag aaagttgctc tttgctaatc
    12781 gctaacgcta aacttttggc aaatcgaccc cttgcttcat caagggataa cacttctcga
    12841 gccgacattt tcagtccacc gctgaaatca tcaaactgta ccgaacctgt tgccacaata
    12901 atttggtctt gttgcatcaa atgcccgaaa ttttctaagg cttccgagaa aagggtaatg
    12961 tccaatttcc ccgagcgatc ttcaatagtc gcaatcccaa tacggcttcc tcgcttggta
    13021 actgccactc gagatgccat aacaattcct gaaaccgtgg tcatttgccc tcggaaagtc
    13081 ggttgcaatt cattcagccg attaggggca tagtgggata attctttgag gaatctacca
    13141 atcgggtgtc cgcttaaata cagcccgagc gtggcacgct ctccatctaa aatcacttgc
    13201 tccgaccatt ttggcgtatt ggcgtaagca ttttgcactt cttccggtgt ctcggtaagc
    13261 acgccaaaca tatcgctctg cccgagttcc tccattttag cgtgctgatc tgaggcttta
    13321 agtgcatctt ccaaattctt catcaaggca gctcggtgag ggcctaattt gtcaaaagca
    13381 ccggacataa tcaggctttc aaaggtgcgg cggttgattt ttttcagatc gacccgagcg
    13441 gttaaatcaa atagatcttt aaatttcccc tctttttggc gagctgccag aattgcctca
    13501 atcggtcctt ccccaacgcc tttaattgct cctagcccgt aaacaatttc gcctttttca
    13561 tttacgctaa aacgatgttt accgctgttt acatcaggcg gcacaacggt taagcccata
    13621 ttcatacact catcgtagaa cccgacaatt ttatcggtat tatccatctc tgaggtcatt
    13681 accgcagcca taaattctgc cggataatgg gcttttagcc aaagtgtttg gtaagaaacc
    13741 aacgcataag ctgctgagtg agatttatta aagccgtagc cggcaaactt ttctaccaag
    13801 tcaaaaattt gaccggcaag tttaccgtca atccctttgg cttccgcccc tttttcaaac
    13861 acttcacgct gtgcagccat ttcctccggt ttcttcttac ccattgcacg gcggagtaag
    13921 tccgctccac cgagtgtata accggcaagc tcttgtgcaa tttgcataac ctgttcttgg
    13981 taaacaatga ccccataagt cggctcgaga ataggtttta agcattcgtg ttggtattgc
    14041 ggatcaggat aagaaacctc ttctttgccg tgtttacggt caataaagtt ctgcaccata
    14101 cctgattcca gcggccctgg gcggaatagt gccaccaatg cgataatatc ttcaaaacag
    14161 tccggcttca ggcgagaaat taagtctttc attccccgtg attcaagctg gaatactgct
    14221 gtagttttag ctgccagcaa cagagcaaag gattttttat catcaagcgg aatggtttca
    14281 atccgaatcg gctctttgcc ttcacgagcc aaacgttcgt taatcatctc caacgcccat
    14341 ttgatgatgg tgagcgtacg caagcctaag aagtcaaact tcaccaatcc tgcatattcc
    14401 acgtcatttt tatcaaaatg ggtaaccggg tgtaagccct cgctatcgca ataaagcggc
    14461 gaaaaatcgg taatcgcagt tggggcgatc accacaccac cagcgtgttt acctgcatta
    14521 cgagttacac cttcgagctt acgagccata tcaataaggt ctttcacctc ttcatcggct
    14581 tcatatagct caggcaattt tggctcagcg gcaaaggctt tttcaagcgt cattcctgga
    14641 tcgggcggaa tgagtttgga gatgcgatcc acaaagctat aggggtgtcc aagcactcgc
    14701 cctacatctc gaatgaccgc tttcgccgcc atagtaccga atgtgataat ttgcgatacc
    14761 gcttgtcgcc cgtaggtttc cgcaacgtgt tcaatcacac ggtctcgccc gtccatacag
    14821 aaatcaacgt caaaatcggg cattgaaaca cgctcagggt tgagaaaacg ctcaaaaagc
    14881 aaatcaaatt ccaatgggtc aagatcggta atcttcaacg cataagccac caatgagcct
    14941 gcccctgagc cacgacccgg tcctaccgga atatcattat ctttggacca ttggataaac
    15001 tccatcacga tcaaaaagta acccgggaac cccatttggt tgatcacatc taattcaact
    15061 tgcaatcttt cgtcataaac cgaccgcttg ttcgcccttt cctcaggatc agggaataga
    15121 aactctaaac gctcttctaa gccttctttc gattttttta ccaagaaatc ttcggtggaa
    15181 agctcaccgg tcgggaaatt tggcaggaaa tactcgccca aacgcacggt aaccgaacag
    15241 cgtttcgcaa tttcaaccgt gtttgccaat gcactcggta aatcggcaaa cagcttgcac
    15301 atttcctctt cagtacggaa atattgtttc ggtgagtatt ttttcgggcg tttcgggtcg
    15361 tctagggtgt agctgtcgtg aatggcaacc cgaatttcgt gtgcgtcaaa gtcatcttct
    15421 ttcaagaaga caacatcatt taccgccact aacggaattg catttttttg tgaaaattcg
    15481 accgcttgtt tgatatagcg ttcttcgtct gcccgtccgg tacggcaaac ggaaaggtaa
    15541 aaatgattcg ggtaatattg ctgataaaaa gctaattttt cttcagcttc ttgtcggttt
    15601 tctttgagta aggctttacc cacatcgccc attcgcccgc cggaaagaat gatgatgccc
    15661 tcattcagct ctgctaacca ttcacgttcc accatcggaa attccacata gccttgctga
    15721 taggctttgg acaaaatttg ggtaatatta tggtagcctt tgttgttctt cgccagtaat
    15781 gtcagctcaa aataatcttc attgccgctc tcggttctaa cataaacatc tgccccgata
    15841 atcggcttaa tccctgctcc caatgcttcg ccatagaatt tcaccaaccc gcaaaagttt
    15901 ataaaatccg ttaagcccat tgccaccata ttattagcgg cagcggtttt aactaaggat
    15961 tttaccttag ctaaaccatt aatcattgag aaatcactgt gaacttttaa atgaacgaaa
    16021 cgacaatctg acataggaat ataatctaag taaagaagtt tcccctcttt agtaaagact
    16081 gtcaggttct aaaaagtgaa gccttctgtg aataatcccg ttgggcgaat gttattcgcc
    16141 cctacaaacg agcattattc ttgctcagag ttacatttaa aatccgaatg taggggcgaa
    16201 ttacattcgc cctttcagaa cctaacaacc ctgctttatc taaagaaggg agttaaaatt
    16261 aatcacgttt tttgcttaac agccaccaaa cgatgatcaa acagagtagg cttggaatta
    16321 atgctccaac cggtataggt aaccaagtgg caattaagct taaattgcca aacacaatat
    16381 ttgccacata gaacataaag ccggcaacaa taccaatcac aattttcgcc cccatcgtac
    16441 tactacgaag cggcccgaaa ataaaggaga tcgcaagtag catcatcacc gccattgaga
    16501 ttggttggaa tacttttcgc cagaaggtga tctcaaaacg tttcggatct tgtccggtat
    16561 cttttaagaa gccgacataa tctgccaagc ctgaaatcga gagcgactcc ggtttgagtg
    16621 aaacaatgcc taatttgcta ggggttaaac tggtttgcca aacttgatct ggctgtttag
    16681 cttgctggat ctgattttct cccacagtcg atttttccac tttagatagc acccagcctt
    16741 tttcatcact gtaattcgca gttcgggctt ttaaaacaga ttgcaattca cgtttgtttt
    16801 gtccttcgcc gaattgataa atcagtacgt tatctaagct tttttcattt ttaatatgat
    16861 taatatagat aaatgaattg ccatctttcg cccaaaaccc gtcttttgtg gcaagcattg
    16921 agccgccact ttgggcaatc gaacgcatat tacgggcaaa ctgctcggtt tgtggcacgc
    16981 cccattcgcc gataatcatc gtaaaaatga ctaacggtaa ggcagttttc atcacggcta
    17041 aaccaatgcg gaaacgggaa aaaccggagg cttgcatcac caccaattca ctaaggctcg
    17101 ctaggttgcc taaaccgagt aatgagccaa gtaatgccgc aatcgggaaa aaggtttcga
    17161 tatctcgtgg aatagttaaa aaagtatagt atgccgcttt taagccatcg taagatccac
    17221 gtcctacatc acgaaactct tccacaaatt taataatcgc cccaagccca atcaacataa
    17281 acagggttaa aaaaatcgcg gataaaatcg ttttgccgat gtagcgttct aaaatattca
    17341 ttgagaaaaa ttgcattatg ccaccgcctt attggttaat ttttttgcac caaattgata
    17401 gcgtaactta gacataaatt tactatccca cgcatttaaa ataacaccta aaactaaaaa
    17461 ggcaactgat accaacggca ttaacacttc cgcattcaac ttgcctgatg cccctgacga
    17521 ttttagcgag ctttgtagta agaaatagat taaataaagc aataacgccg gcagtagttt
    17581 agcaaaacgc ccttgacgag ggtttacact actcattggc accgctaata atgccattaa
    17641 cggtacagct aaaatcaaag caagccgcca atgaagctcg gcttttgctt ccggcgtttt
    17701 ggtttccatt aatttcttga aatcggctcg ttgcactaat ttctcatcag attcaacgtc
    17761 ttgatagcct aaataagcag aataattctc aaaatgtgaa atacggaaat cggcactttg
    17821 tggtgtacct tcaaaacgct tgctctctgc caaggttaag acttgatccc catttggcaa
    17881 accttgcaac gtgccggatt cagcaacaac cactgaaggt ttgtttttct tctgctgttc
    17941 cggctggaaa acgtagatat catttagact gttattttca attttatcaa taaataacac
    18001 atagccaccg gcagacataa attgtccggc cgaaagagcc gcaaaacgtg ggttggcttt
    18061 cgcttccgct aacatttcac tctgtttatt aattgcccaa ggcgtaagcc aaaacacgtt
    18121 ataaaccgct aatgcagtgg taaaaatcga gagaaacatc gccactttaa ccaatagccc
    18181 ttgcccgata ccacaagccc gcattacggt aatttcactt tcggcataaa accgcccgag
    18241 ggttaataac aaggcgataa acagtgagag tggcagcatt aattgtgcca ttgtcggcat
    18301 acctaagcct aataagctca ccactaaatc tgccggtact ttaccactca cagcagaact
    18361 tagaacacgc actaattgct gacaaaaaaa gatcagtaat aagataaaca atatcgccaa
    18421 ttggctttta aatatctctt tggttaaata tcggcttaag atcacgtttt ttctctataa
    18481 catttacaaa tctgaatacc gctaaacaca agcggtctat ttttgttaat tttttgcaaa
    18541 gagcgcaaaa taacttattc gaatcgctaa taataccttt ttcatacaaa aaaatcacct
    18601 atagaaaata tcacgcacgg caaaaccgtg cgtaagtatt attgtttttt cgattttttc
    18661 gatgatgttg ctttatgagc ttcaagaatt aatttcttac cttggctatc ttcaatttca
    18721 atcttaggtg gtgcttccgt attttgatga aggatagcta taatattttc accttgtgca
    18781 atatcttcaa agtttccttt cacacgtaac ttagccgcat caacactagt gtcaaatact
    18841 aagaatgtac ctaatgcggt aactctaata tttttaagcc ctatattgtc cttcgttacc
    18901 gttttatatt gaggtgcttt tttccctttt gcagccataa tcttctcttg attagtcgca
    18961 atattaagaa tattttgaac tttagtcgct tctgctgtag attgtgcctg ttctgttaaa
    19021 tttggttctc catcaaaaat aacatcccca acaaattgca cctctttagg ctcattttgc
    19081 aataatggtg caacgtcttt agctgaaaca accaaaaata accgttcacc tattttttca
    19141 aaacggacat caatattggt taattgtgat tcaatttgtt cattaattgt cttgatcgca
    19201 gtttctgtct cattttgaag cgatatttta cgtgtaatat caacatcata agcaataaaa
    19261 ccaactttta aacgttgtga taactgctga agtaaattgc ccacatttcc atcataagaa
    19321 agacgtaact ttgcctgctc atagcgacta atttgagcat catcattagc gaaaatagtg
    19381 ttttgagtcc ctaagccgat tagaccaaca acaaacgttt taccccaaaa gcgcatcatt
    19441 tttatctcca attattctct taaatctaaa aaactc
//
LOCUS       DANLXF010000016        19268 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.016, whole genome shotgun sequence.
ACCESSION   DANLXF010000016 DANLXF010000000
VERSION     DANLXF010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 19268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19268
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.016"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            298..879
                     /gene="rsxA"
                     /locus_tag="PXD66_000340"
     CDS             298..879
                     /gene="rsxA"
                     /locus_tag="PXD66_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007525505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HDL6318675.1"
                     /translation="MVDYILLVISTALINNFVLVKFLGLCPFMGVSKKVETAIGMGLA
                     TTFVLTVASLSAYLMETYLLEPLNAQFLRTLVFILVIAVIVQLTEMIVHKTSPTLYRL
                     LGIYLPLITTNCAVLGVALLNVNLANNLVESVLYGFGAAAGFSLVLVLFAALRERLAA
                     ADVPRPFQGASIALITAGLMSLAFMGFTGLVKL"
     gene            879..1496
                     /gene="rsxB"
                     /locus_tag="PXD66_000341"
     CDS             879..1496
                     /gene="rsxB"
                     /locus_tag="PXD66_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HDL6318676.1"
                     /translation="MLDLPIIAYILIAIAVIALIFGAVLGYSSIKLKVESDPIVDQID
                     ALLPQSQCGQCGYPGCKPYAEAIANGDVITKCVPGGQPLVVNLAELLGVEVPPMEGMA
                     EPEMKVAFVIEDLCIGCTKCIQACPVDAIIGTNKAMHTIIPDLCTGCELCVAPCPTNC
                     IEMRPIKPTTQSWNWKFDQDLIIPIVNTTELQRKLVTGGGKNEQS"
     gene            1483..3777
                     /gene="rsxC"
                     /locus_tag="PXD66_000342"
     CDS             1483..3777
                     /gene="rsxC"
                     /locus_tag="PXD66_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HDL6318677.1"
                     /translation="MSNPNLVIERIRQNKETGKLWDFPGGIHPMENKSQSNSKPIRTL
                     NLPKFFYVPLVQHSGWAGELLVKVGDVVLKGQPLTKGDHFRQLPVHSPTSGRVIGIEP
                     YVSAHASGLPEITVIIETDGKDKWVERQPIEDFLSLTSDQIIQKVYQYGIAGLGGAVF
                     PTATKLSLADKRCKLLVINGAECEPYITCDDRLMQDFTADLIEGIRILRYVLRPEEVV
                     IAIEDNKPHAIQALETALKGSNDMFVRAIPTKYPSGASDQLIQVLTGLEIPAGKRTIE
                     MGIVMHNVGTAFAVKRAIIDDEPLIERVVTLTGDKIRNKGNVWARLGTPIQHLLEQVD
                     YQADKRFPVFLGGPMMGFILPALQAPITKTANCLIAPDHFEYAPPEPERSCIRCSSCS
                     DACPVGLLPQQLYWFARAEDHDKSKEYHLDACIECGVCAYVCPSYIPLIQYFRQEKAK
                     IEEIEEKARKAEEAKIRFEAREARLQKERDARTARISQAAEKRREEVANAGGVDPVKA
                     ALERLKAKKAVETVQEIPAEVQVKPNGEPDNSDLMAQRRARRLAKQAEQAVEKSENVT
                     NALIGANNDLPLQSMNETASDKSVGANNYSPEAESEDPRKAAVAAALARAKAKKQAQQ
                     AVEKSENITNAVIGVNNDLPLQSMNETVSDKSVGANNHSPETESEDPRKAAVAAAIAR
                     AKAKKQAQQAVEKPEDIANAVIGVNNDLPLQSMNETTSDKSVGANNHSPKTESEDPRK
                     AAVAAAIARAKAKKAAQQEKSNNV"
     gene            3770..4405
                     /locus_tag="PXD66_000343"
     CDS             3770..4405
                     /locus_tag="PXD66_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HDL6318678.1"
                     /translation="MFSELIPALPWSFLFIQDLIGTIVFAISGAIAARQHKMDIFGMF
                     ILAFVTGVGGGTLRDMMIGSTPVFWMKQPIYVLMITLAVVITAIFRNQISRKSWQTGL
                     LVFDAIGLGVFTVIGVQKGLDFGLHPLICVALGGVTGCFGGLIRDILRNEVPIILQKE
                     VYVTASLIGGVIFVAANSVGLESDWVYLATVFTVIIIRLLAIRFNLNLPRL"
     gene            4451..5506
                     /gene="rsxD"
                     /locus_tag="PXD66_000344"
     CDS             4451..5506
                     /gene="rsxD"
                     /locus_tag="PXD66_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HDL6318679.1"
                     /translation="MFKMVSSPHTHSSNLTANFMLWVIAMMLPAFGVQLYYFGYGVLI
                     QTLLAVSLAIVIEIGIAKLRKKTTAFYLADLSGILTALILAMSIPPYAPYWLVLIGVI
                     VALLLAKHSYGGLGQNLFNPAMVGYALLLVSFPVQMTAWIVPIELLSEPPTFRDAISL
                     IFYGVTSDGFSIHQLVNSVDGIAQATPLDSMKSSLAKSGFDAVMNTPIFNGLFATGWL
                     QLNLAFLFGGLVLIYKRIIHWQIPVAMLATFAFFSGFTELFSQQSHLHFVSHFFSGAM
                     MFGAFFIATDPVTASITPRGKLIFGGLVGLLVYLIRYYGNYPDAIAFSVLLANICVPL
                     IDHYTQPRLYGTKLDNR"
     gene            5506..6138
                     /gene="rsxG"
                     /locus_tag="PXD66_000345"
     CDS             5506..6138
                     /gene="rsxG"
                     /locus_tag="PXD66_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HDL6318680.1"
                     /translation="MNTSKITLNYALILGFIALLCTAVSTGVYLLTKGKIDDVTAMQQ
                     RKLLEEVVPKSHFDNDVLATCKLVQLENAPYLNRIYVAKKSENLTAYLVQGTAPDGYS
                     GDIVLLMGIEPNGNILGVRTLEHKETPGLGDKIETRVSDWILSFTNQLFSLENEADWN
                     VKKDGGKFDQFTGATITPRAVVNHIRQSAKWVVTELAQNPTMFNQFKACK"
     gene            6147..6830
                     /locus_tag="PXD66_000346"
     CDS             6147..6830
                     /locus_tag="PXD66_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HDL6318681.1"
                     /translation="MEREQQIPVTNIDVAPKQSSVWKTIFADGVWKNNGALVQLLGLC
                     PLLAVSNSVTNALGLGLATMFVLICTNITVSLFRKITPHDIRIPIYVMVIATVVTAVQ
                     LLMNAFAFPVYQSLGIFIPLIVTNCIVIGRAEAYASKNEVKFSAFDGFSMGLGMTFSL
                     VVLGALREIIGNGTLFSGLDLLLGDWAKALRIDVLHIDSGLLLAILPPGAFIGLGVIL
                     AVKNIIDKR"
     gene            7271..8221
                     /gene="lpxM"
                     /locus_tag="PXD66_000347"
                     /gene_synonym="msbB"
     CDS             7271..8221
                     /gene="lpxM"
                     /locus_tag="PXD66_000347"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249087.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HDL6318682.1"
                     /translation="MTRITAQDGYTHKFLWSFLHPKYWGVWLGVLGLVILAYVPVRIR
                     DKLSAFVGKMAYRYLKQKNKKGYHRAKVNLRYCFPDWSEKKREEVVEKMFITVAQTML
                     AIGETAIRPASYLQKRCEFTGFENVVKAKESGKNVIMLVPHTWSIDMAGVAMFSLGYP
                     MTSMYNPHRNALVDWLWNITRERLGGRMHTRQNGIKAFLADVRKGDVGYFLPDEDFGE
                     ELSVYADFFATEKATLPGLNKMARVANAEVIPMFSIYNAEKAKYEMEILPPLEFSGDP
                     IQSAREMNRVIEYFVEKNPEQYVWILRLLKTRRNREDIYL"
     gene            8349..8888
                     /gene="apt"
                     /locus_tag="PXD66_000348"
     CDS             8349..8888
                     /gene="apt"
                     /locus_tag="PXD66_000348"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDL6318683.1"
                     /translation="MNQLDLIKSSIKSIPDYPKAGIIFRDITSLLEVPTAFQATIDAI
                     VAEFKDKGITKIVGTESRGFIFGAPVALALGVPFVLVRKPKKLPREVISQSYTLEYGE
                     DTLEIHKDAVSANDNVLVIDDLLATGGTIDATAKLIRRLGGNVEHAAFVIWLPELGGK
                     ERLEKEGVKSFSLVSFEGH"
     gene            8979..11036
                     /gene="dnaX"
                     /locus_tag="PXD66_000349"
     CDS             8979..11036
                     /gene="dnaX"
                     /locus_tag="PXD66_000349"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDL6318684.1"
                     /translation="MSYQVLARKWRPQRFSEVVGQQHVLSALENSLREDRLHHAYLFS
                     GTRGVGKTSIARLFAKGLNCVNGITAEPCGECANCKAIEEGHFIDLIEIDAASRTKVE
                     DTRELLDNVQYKPTVGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEYVKFLLATTDPQ
                     KLPITILSRCMQFHLRALEQSQIARHLEFILNAENIPFEPLALEKLAKAAQGSIRDSL
                     SLTDQAIAMSNANITLAIVSQMLGLIDDNQPLELVQATAQADGEKAMAIIEQIAAKGV
                     DWQQLLSDVAEVLHKIAMLQLVKTPTTEETLVHFLAKQIPPEDVQFFYQLILNSKKEL
                     PFAPEQRAGVEMAILRALAFHPKQAEKIATVAVEKVTQPNHSVSAVTTAKPQNQISQL
                     RENFEQNQQKTVTVANTTTAVESIAQPPQAVDTAISPSAPATLSPAMAALEARKKLQA
                     QQANLAQTEKKKPELKAPQVIAKKSANQTASLQERFISLAENQHKAVLQVSKKTESAP
                     KSVEDEDYRWTWLNPELENQADSAKPSDIKQAILQERTPELVAKTIHLSCQQDKWCDI
                     ANSLNLGGFSRQIALNSYLAEQNEQHLALVLKPNMAHLDNPESRKNLEAALAEKGYSY
                     ELTIGDNSEQQTPLEIRRAIFKQLTEAAKSALLNDEKLMLLRNAFDAQIDESTIRAVA
                     ETE"
     gene            11042..11950
                     /locus_tag="PXD66_000350"
     CDS             11042..11950
                     /locus_tag="PXD66_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL6318685.1"
                     /translation="MKALELIDLVKTYPTGVKAVKGINLVVEQGDFYALLGHNGAGKS
                     TTIGIISSLVNKTSGSVKVFGYDLDTQKVELKHQIGLVPQEFNFNQFEKVIDVLVQQA
                     GFYGIPRSEALVRAEKWLKLLDLWEKRTHLTRELSGGMKRRVMIARALMHNPKLLILD
                     EPTAGVDIELRRSLWDFLRDLNNQGTTIILTTHYLEEAETLCRHIGIIQHGELIENTS
                     MKALLAKLESEVFVLDLQNSEQNQPLVIQNYPHKWLDENTLEVNVKRQQGLTNLFSQI
                     SAQGAEVLSMRNKSNRLEELFINMAQ"
     gene            11952..12713
                     /locus_tag="PXD66_000351"
     CDS             11952..12713
                     /locus_tag="PXD66_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL6318686.1"
                     /translation="MNLIGFYTLAYKECRRILRIWRQTLVPPVITTTLYFLIFGTLIG
                     GRIGEMNGVGYTQFIAPGLIMMTAITASYVNTASSFFLSKFTRDFEEMLISPLSTHNI
                     IWGYVTGSILRAGLAATLVMCVAMVFVSFEIYSVAIIVLTLLLTIIAFALGGVINAVF
                     ARSFDDVGLIPTFVLTPLTYLGGVFYSISLLPEFWQTVSKFNPIVYMINGLRYGFLGI
                     SDVPLGYTFAVLTSLCIGLYIIAYSLIQRGVGLRS"
     gene            12734..13489
                     /gene="modA"
                     /locus_tag="PXD66_000352"
     CDS             12734..13489
                     /gene="modA"
                     /locus_tag="PXD66_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL6318687.1"
                     /translation="MKALKKLAITMALTLSGTAFSQEITVFAAASMTNVLQDIQQDFE
                     KAYPQDKITFSFASSSVLAKQIEQDAPADIFISADQKWMDYVAEKQPEKVKHRRNLVR
                     NELVLIAPTESNIEPKAIQAVDFAKELANSYLSVGDPNHVPAGKYAKKALSHYQLWES
                     VEPRLARAKNVRDALSFVERGESPLGIVYSTDAKVSNKVKVVAVFPPESYGEIVYPAA
                     AVSEKASAATFLDFLKTPKAKAKFEAAGFYPVN"
     gene            13673..14365
                     /gene="modB"
                     /locus_tag="PXD66_000353"
     CDS             13673..14365
                     /gene="modB"
                     /locus_tag="PXD66_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDL6318688.1"
                     /translation="MLNFTPQELNAIVLSLKVASIAVVLALPLAVWVAWLLARKQFWG
                     KNLLNGIMHLPLVLPPVVIGYLLLISMAKRGVIGQYLWEWFHFSLSFSWKGAVLASMV
                     MAFPLMVRSIRLAFEAVDPKLEQAARTLGAIPLKVFFTLTLPLSFSGIIAGGVLGFAR
                     SLGEFGATITFVSNIPNQTQTIPAALFTFIETPDGEMAAARLCLVAILISLIALFCSE
                     WLAERQKRYAQT"
     gene            14352..15407
                     /gene="modC"
                     /locus_tag="PXD66_000354"
     CDS             14352..15407
                     /gene="modC"
                     /locus_tag="PXD66_000354"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HDL6318689.1"
                     /translation="MLKLKLLQQLGNLTLNVNLDLPNKGITAIFGRSGAGKSSLINLI
                     AGLSTAQAGFIELNGQVLFDSQNGINLPPEKRNIGYVFQEHRLFPHYSVEKNLKYGYK
                     RSDSADFLQIVELLGIAPLLNRYPASLSGGEKQRVAIGRALLSSPNILLMDEPLSALD
                     LPRKQELLDYLDKLSARLEIPILYVSHSLEEVVRLADHLVLLENGKVTAFDTLSAVWH
                     SSAFSAWQPNHQRISLLELPVEETLPHYNMLGLSLNAQRLWVNQLPRHQIGEKLRVTI
                     ASKEVSISLQKPTQSSIRNVLKGNVRKIETHLDRVDLAIQVENQQIWSTISLWSFNEL
                     AIQPEQEVYLQIKSVSL"
     gene            15462..15896
                     /locus_tag="PXD66_000355"
     CDS             15462..15896
                     /locus_tag="PXD66_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HDL6318690.1"
                     /translation="MPIVNQSALVPYSAEQMYQLVNQYEKYPEFLSGCVSTKTLSTGE
                     NELKAELHIQKLGISQTFSTHNKMTPPYKIEMALIEGPFRHLHGAWTFTPFDEQSCKI
                     ALQLNFEFSSPIVAMVFGKVFNELTLKMVNAFKQRAKEVYGV"
     gene            15886..16221
                     /locus_tag="PXD66_000356"
     CDS             15886..16221
                     /locus_tag="PXD66_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HDL6318691.1"
                     /translation="MVSEQRQNPEEQKSEEQKINVEVLYAYPDKYFLKKLQVPQNATI
                     QTIILLSGVLEKYTEIDLRENKVGIFSRPAKLNETVEEGDRIEIYRPLIADPKEIRRK
                     RAAEQAEKK"
     gene            16425..17714
                     /locus_tag="PXD66_000357"
     CDS             16425..17714
                     /locus_tag="PXD66_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL6318692.1"
                     /translation="MDIRQKIAHNKMTSFQWLVVGLTVLLNMLDGFDLMALAFTAKSI
                     REELQLDGAAIGSLMSAGLFGMAIGSLFLSPLADKFGRRPLLLIGTGLATLGMLLTYF
                     ATSAFEIGVWRVLTGLGVAAILTGTNVLVSEYSSQKWRGLAIAIYASGFGIGAMLGGM
                     SAVLLQGEYGWRAVFLVGSILTGAVFVLLFFVLPESVEFLISKHPVNAKARLNQIAVK
                     LGLGNGWELPEIEAKSKSKVAITRLFEPEYRRNTLLIWLAFIAISASFYFISSWTPAL
                     LEEAGMPKTQSQTVGMAISVGGTIGSLIFGFLVSRWAAKHILQAFSILAASAVVAFVF
                     AGNLGLAITLAILVGGLINGCITGLYTINPTLYAPDFRSTGVGTAIGVGRLGSIVSPT
                     LAGILLDAGWKKNELYLGAAVVLLIAAFTVHFLKQRK"
     gene            complement(17789..19243)
                     /locus_tag="PXD66_000358"
     CDS             complement(17789..19243)
                     /locus_tag="PXD66_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HDL6318693.1"
                     /translation="MSEISTLAPQLLWQWFDKACAIPHPSYHENELAEFIVNWAKSKN
                     FFVERDEVGNVLIRKPATKGMENRQTVALQAHLDMVPQANTGTVHDFTKDPIQPYIDG
                     EWVKAKGTTLGADNGIGLASCLAVLDSDDLAHPTIEVLLTMTEEAGMEGAIGLRPNWL
                     TADIMINTDTEENGEIYIGCAGGENVNVTLPIFLVPHQQDSAVTVTLKGLNGGHSGCD
                     IHTTRANAIKVFARILEQAHNEVEFQIAEIQGGSVRNAIPREAHATLVFSAKNQQKLT
                     ACLSQTAEQVKAELKIAEPNFQFLIEEAQLPEQAFDLASTARVIHFLNVMPNGVVRNS
                     DVVENVVETSLSIGVLNTEASEVKATILARSLIEGGKEEIRSKIRSLTALVGAEVEFS
                     GNYPGWEPNPNSKITPLTKAIYDEILGYESQIKVIHAGLECGLINKIYPNMDFVSIGP
                     TILNAHSPDEKVHIPAVEIYWKLLTKLLEQAPEK"
BASE COUNT         5327 a         4059 c         4391 g         5491 t
ORIGIN      
        1 gagtgagact ttttaggatt aacttagaaa atcaagctcc tttcaccttt catgctgtat
       61 agcttagtcc ccacattctc cattctacca gagatagaaa caaaggtaac aagatcatat
      121 ttgatatggt atgtttacta gaagtacctc cgatttctta ttgtgaatga gccgaaaaca
      181 agagataata taacgtttat ttttaatgag atgctttaac aaacggtcag attttctctg
      241 ttttttgcaa aataaaatgc gtttttgacc gcttgtatat ttaattttgt ggaatttatg
      301 gtcgattata ttctgttggt catcagcacc gcccttatta ataactttgt cttagttaaa
      361 tttctagggc tttgtccgtt tatgggagtg tcgaaaaagg tggaaactgc tattggtatg
      421 gggttggcga caacctttgt gctgacggtt gcctcgctct ctgcttattt gatggaaacc
      481 tatttgcttg agccgttaaa cgctcaattt ttgcgtacct tggtgtttat tttagtgatt
      541 gcggtgatcg tgcaattaac ggaaatgatt gtgcataaaa ccagcccgac actttatcgc
      601 ttgcttggga tctatttacc gctgattacc accaactgtg ccgtattagg ggtggcatta
      661 ttaaacgtga atttagccaa taacttggtg gaatctgttc tttatggttt tggagccgct
      721 gccggtttct cgctggtgtt agttttattt gcggctctgc gtgagcgttt ggctgccgct
      781 gatgtgcctc gtccgttcca aggagcatca attgctctga ttaccgcagg cttgatgtcg
      841 cttgccttta tgggctttac aggattggtg aaactctaat gcttgatttg ccaattatcg
      901 cttatattct tatcgctatt gctgtgatcg cactgatttt cggggcggtt ttaggctatt
      961 cctcgattaa actgaaagtt gaatccgacc cgattgtcga tcaaattgat gcccttttac
     1021 cacaaagcca atgcggtcaa tgtggctacc cgggctgtaa gccttatgct gaggcgattg
     1081 ctaatggtga tgtgatcact aaatgtgtgc cgggtggaca acctttagtg gtgaatcttg
     1141 ccgaattgct tggggtagaa gtgccgccaa tggaaggtat ggcagagcct gagatgaaag
     1201 tggcttttgt gattgaagat ctctgcattg gctgtactaa gtgcattcag gcttgcccgg
     1261 ttgatgcgat tatcggcacg aataaagcaa tgcacaccat tattccggat ctttgcaccg
     1321 gctgtgaact ttgtgtcgcc ccttgcccga ccaactgtat tgaaatgcga ccaattaaac
     1381 cgacaaccca atcttggaac tggaaatttg accaagattt aattattccg attgtgaata
     1441 ccactgaatt gcagagaaaa ttagttaccg gcggaggcaa aaatgagcaa tcctaattta
     1501 gtgattgagc gaatccgcca aaataaagaa acgggtaaac tgtgggattt cccgggcggt
     1561 attcacccga tggaaaataa atcccaatca aacagcaagc caattcgcac cttaaattta
     1621 ccgaagtttt tctatgtgcc actggttcaa cattctggct gggcagggga attattagtc
     1681 aaagtcggcg atgtggtatt gaaagggcag ccgctcacga aaggcgatca cttccgtcaa
     1741 ctgcccgtac acagcccaac ctccgggcgg gtgattggga ttgagcctta tgtttccgct
     1801 cacgcctcag gtttgcccga aataacggtg attattgaaa ccgatggtaa agataaatgg
     1861 gtggaacgcc agccgattga ggatttttta agcctaacaa gcgatcaaat tatccaaaaa
     1921 gtttaccaat atggcattgc cggtttaggc ggtgcggtat tcccaaccgc aacaaaatta
     1981 agtttggcgg ataaacgctg taaattactg gtgattaacg gggcagaatg tgagccttac
     2041 atcacctgtg acgatcgctt aatgcaagat ttcactgcgg atcttatcga aggtattcgc
     2101 attctgcgtt atgtgcttcg cccggaagaa gtggtgattg cgattgaaga caacaaaccg
     2161 cacgctattc aagctttaga aaccgcttta aaaggctcga acgatatgtt tgtgcgggcg
     2221 atcccgacta aatatccctc cggagcttct gatcagctga ttcaagtgtt gaccggactt
     2281 gagattccgg ctggcaaacg cactattgaa atgggcattg tgatgcacaa tgtcggcacg
     2341 gcgtttgcgg ttaaacgggc aattattgat gatgaacctt tgattgaacg ggtagtgacg
     2401 ctgacaggcg ataaaattcg caacaagggg aacgtgtggg caagattagg cacgccaatt
     2461 cagcacttgt tggagcaggt agattatcaa gccgataaac gctttccggt gtttttaggc
     2521 ggaccgatga tgggctttat tttgcctgca ttgcaagccc cgattaccaa aactgcgaac
     2581 tgcttgattg cccctgatca ttttgaatat gcaccacctg aaccggagcg tagttgtatc
     2641 cgctgttcaa gctgttccga tgcttgcccg gtggggttat tgccacagca actttattgg
     2701 tttgcccgtg cagaagatca cgataaatcg aaagaatacc atttagatgc ctgcattgag
     2761 tgtggcgttt gtgcttacgt ttgcccaagc tacattccgt taatccaata tttccgccaa
     2821 gaaaaagcaa aaatcgagga aattgaggaa aaagcccgca aagcggaaga ggctaaaatc
     2881 cgctttgagg cgagagaagc tcgcttacaa aaagaacgag atgccagaac tgctcgcatt
     2941 agccaagcgg cggaaaaacg ccgtgaagaa gtggcaaatg caggtggtgt tgatcctgta
     3001 aaagcggctc ttgaacgcct gaaagcgaaa aaagcggttg aaacagtcca agaaataccg
     3061 gcagaagtac aggtaaaacc taacggcgag cctgataaca gcgatttaat ggcacaacgc
     3121 agagccagac gtttagccaa acaggcagaa caagcggtcg aaaaatcgga aaatgttaca
     3181 aatgccttga ttggggcaaa taatgatttg cctctacaat caatgaacga aactgcatcg
     3241 gataaatctg taggggcgaa taactattcg cccgaagcag aaagcgaaga cccacgcaaa
     3301 gccgccgttg cggctgccct tgcccgagcc aaagccaaaa aacaggcaca gcaagcggtc
     3361 gaaaaatcag aaaatattac caatgccgtg attggggtaa ataatgattt gcccctacaa
     3421 tcaatgaacg aaacggtatc ggataaatct gtaggggcga ataaccattc gcccgaaaca
     3481 gaaagcgaag acccacgcaa agccgccgtt gcagcagcta tcgctagagc taaagcgaaa
     3541 aaacaagctc aacaagcggt cgaaaaaccg gaagatattg ccaatgctgt gattggggta
     3601 aataatgatt tgcccctaca atcaatgaac gaaactacat cggataaatc tgtaggggcg
     3661 aataaccatt cgcccaaaac ggaaagcgaa gacccacgca aagccgccgt tgcggccgcc
     3721 atcgccagag ctaaagcgaa aaaagccgct cagcaggaga aaagcaacaa tgtttagcga
     3781 gctaattccg gcattgccgt ggtcgttcct gtttattcag gacttaatcg gcactatcgt
     3841 gtttgcgatt tcgggggcaa ttgctgcccg ccagcacaaa atggacattt tcggtatgtt
     3901 tattttagcg tttgtgaccg gggtcggggg cggtacgcta agggatatga tgattggctc
     3961 cacgccggtg ttttggatga aacaaccgat ctatgtgctg atgattaccc ttgcagtggt
     4021 gattaccgct attttccgca accaaatttc ccgcaaaagt tggcaaaccg gcttgctggt
     4081 gtttgatgcc atcgggcttg gcgtatttac cgtgattggc gtgcaaaaag gcttggattt
     4141 cggcttgcac ccactgatct gtgtggctct gggcggtgtg acaggctgtt ttggtggctt
     4201 aatccgtgat attttacgca acgaagtgcc gatcattttg caaaaagaag tgtatgtgac
     4261 cgccagcctt atcggtggtg tgatttttgt ggctgcaaac tcagttgggc tcgaaagcga
     4321 ttgggtttac cttgccaccg tgttcaccgt aattattatc cgcctgctgg caatacgctt
     4381 taatttgaat ttgccgagac tttaataagc ggtcattttt ttgcaatttt ttactaaaaa
     4441 ggttttatag atgtttaaaa tggtcagttc cccccacact cattcgagca atctcactgc
     4501 gaattttatg ctgtgggtga ttgcgatgat gttacctgct tttggggttc agctttatta
     4561 tttcggctat ggtgtgctga ttcaaacgtt gcttgccgtt agccttgcga ttgtgattga
     4621 aatcgggatt gcaaaattac gcaaaaaaac aaccgctttc tatttggctg acttatcagg
     4681 tattttaacc gccttgattt tggcgatgtc gattccgcct tatgctcctt attggcttgt
     4741 gttgattggc gtgattgtgg cgttattatt ggcaaaacac agttatggtg gcttggggca
     4801 gaatctattt aacccggcaa tggttggcta tgcgttgttg ttggtttctt tcccggtgca
     4861 aatgacggcg tggatagtgc cgattgagtt attaagtgaa ccaccgacat ttcgtgatgc
     4921 gatttcgttg attttttacg gggtaactag cgatggtttt agtattcatc agctagttaa
     4981 tagtgttgat ggcattgcac aagctacccc gcttgatagt atgaaaagca gccttgcaaa
     5041 atcaggtttt gacgcggtaa tgaatacgcc gattttcaac gggctgtttg cgaccggttg
     5101 gctacaactg aaccttgcgt tcttattcgg cggtttggtg ttgatttata aacggattat
     5161 ccattggcaa attccggtag caatgttggc aacttttgcc ttttttagtg gattcaccga
     5221 gctgtttagc caacaatcgc acttgcattt tgtgagccat ttctttagcg gtgcgatgat
     5281 gttcggggca ttttttattg cgaccgaccc ggtaacagcc tccattaccc caagaggaaa
     5341 actgattttc ggtggcttgg tgggattatt ggtttatcta atccgctact acggaaatta
     5401 ccctgatgcg attgcatttt cagtgttatt ggcaaacatt tgcgtaccgt taatcgacca
     5461 ttatacgcag ccgagattat acggcacaaa attggataac agataatgaa cacctcaaaa
     5521 attaccttaa attacgcctt aattttaggc tttattgcct tgctttgcac cgctgtttca
     5581 accggtgtgt acctgcttac caaaggcaag attgatgatg taaccgcaat gcaacaacgc
     5641 aaattattgg aagaggttgt gccgaaaagc cattttgata atgatgtgtt ggcgacttgc
     5701 aaactggttc agctcgaaaa tgccccttat ttgaaccgta tttatgttgc aaaaaaatcg
     5761 gaaaatctga ccgcttacct agttcaagga acggctccgg acggctattc cggcgatatt
     5821 gtgctattga tggggattga gccgaacggc aacattttag gcgttcgcac ccttgagcat
     5881 aaagaaactc cggggctggg agataaaatt gagacccgag tgtcggattg gatcttatct
     5941 tttaccaatc aactttttag tttagaaaac gaagcggatt ggaatgtaaa aaaagatggc
     6001 ggcaagttcg accaatttac cggtgcaact attacacctc gtgcggtggt caatcatatc
     6061 cgccaaagtg cgaaatgggt ggtgacagag ctggctcaaa acccgacaat gtttaatcaa
     6121 tttaaagcgt gcaaataggg ggagaaatgg aaagagaaca acaaatccct gtgacgaata
     6181 ttgacgttgc cccgaaacaa tccagcgttt ggaaaacgat tttcgctgat ggtgtgtgga
     6241 aaaataatgg tgctttggtg caattattag ggctttgccc actactggcg gtttcaaaca
     6301 gcgtaaccaa tgcacttgga ctaggcttgg caacaatgtt tgtgctaatt tgcaccaata
     6361 tcacggtttc cctgtttcgg aaaatcactc cacacgatat tcggattccg atttatgtga
     6421 tggtaattgc aacggtggta acggcagtac agcttttaat gaatgctttt gctttccctg
     6481 tttaccaatc gctcgggatt tttattccgc tgattgtaac caactgtatc gtgattggac
     6541 gagcagaagc ctatgcctca aaaaatgagg tcaaattctc ggcttttgac ggcttttcaa
     6601 tggggctggg aatgactttt agccttgtgg tgctaggcgc gttgcgtgaa attattggca
     6661 acggcacgct gttttcaggc ttagatctac tccttggcga ttgggcaaaa gccctacgca
     6721 ttgatgtatt gcatattgat tcaggtttac tactggcaat tttacctccg ggggctttta
     6781 ttggacttgg ggtgatattg gcggtgaaga atattattga taagagatga tgtgttatgt
     6841 cacttttctt tgcttgtgca aagaaaagta accaaaagaa agcacaccct gctttgttgc
     6901 tatcctcact tggttgaaat tttcttaacg aaaaataacc ctgatgttcg cttcgctctc
     6961 gctcgagtta ttttcctaaa aatttcaagt cgttcgggca actcagaagg gaacccgatc
     7021 cgaccataca agcggtgatt tttttggaaa attttgcaaa atgatgctct gccgggttcc
     7081 cttctgaacc gcccgaacga ctttgaattt gtaggaaaaa ttccttgttt gagcagcgaa
     7141 gcggcgagtt tggaattttt tcgttaagca aattcaaacc aagcgaagat agcggtgaag
     7201 tagggttgcc tttctttggt tactttcttt ggcgaagcaa agaaagtgac aaataatgag
     7261 gaattataga atgacacgaa ttaccgccca agacggctat acgcacaaat tcctatggtc
     7321 ttttctacac ccgaaatatt ggggcgtatg gttaggtgta ttaggattag tgattttagc
     7381 ctatgtgccg gtgcgaatta gggataaatt atccgctttt gtgggtaaga tggcgtatcg
     7441 ctatctcaag cagaaaaata aaaaaggcta ccaccgtgct aaagtgaatt tgcgttactg
     7501 ttttcccgat tggagtgaga aaaagcgtga agaagtggtc gagaaaatgt ttatcacggt
     7561 agcacaaacc atgttggcga ttggcgaaac tgcaatccgc ccggcctctt atttacaaaa
     7621 acggtgtgaa tttaccggct ttgaaaatgt ggtgaaagcc aaagagagcg gtaaaaacgt
     7681 gattatgtta gtgccacata cttggtcgat tgatatggcg ggggtggcga tgttctcgct
     7741 cggttatccg atgacatcaa tgtacaatcc acaccgtaat gcgttggtgg actggctgtg
     7801 gaatattacc cgtgagcgtt taggtgggcg aatgcacact cgtcaaaatg ggattaaagc
     7861 gtttttagcc gatgtgcgta aaggtgatgt tggttatttt ctgcccgatg aagatttcgg
     7921 cgaagagcta agtgtgtatg ccgacttttt tgccaccgaa aaagccactt tgccgggctt
     7981 aaataaaatg gcaagagtgg caaatgcgga agtgatccca atgttctcga tttataatgc
     8041 ggaaaaagca aaatatgaaa tggaaatttt gccaccgctt gaattttcag gagacccaat
     8101 tcaatccgcc cgagaaatga accgagtaat tgaatatttt gtggagaaaa accccgagca
     8161 gtatgtttgg attttacgtt tgcttaaaac cagacgaaac agggaagata tttatctata
     8221 atatagggaa aaggaaactg aaaagtaagc tgtactatca aacgtaaggt tatcaatttc
     8281 tccttgaaat ttctgctgtt tttctataga gttttccagt tattttttta ttacacgtag
     8341 gtcaaacaat gaaccaatta gatttaatta aatcttctat caaatccatt ccagattatc
     8401 cgaaagcagg gattatcttc cgtgatatca cttcactttt agaagtgcca actgcattcc
     8461 aagccaccat tgatgccatc gttgcagaat ttaaagacaa aggtattact aaaattgtcg
     8521 gcacagaatc tcgcgggttt atttttggtg cccctgttgc attagcgtta ggcgtgcctt
     8581 ttgtgttggt tcgtaaaccg aaaaaattac cacgtgaagt gatttcgcaa tcttacacgc
     8641 ttgaatatgg tgaagacacg ctagaaattc ataaagacgc agtgagtgct aatgataatg
     8701 tattggtcat cgacgattta ctcgcaaccg gtggtactat tgatgcaact gcaaaactaa
     8761 ttcgtcgctt aggtggtaac gtagaacacg ctgcctttgt gatttggctg cctgagttag
     8821 gtggtaaaga gcgtttggaa aaagaagggg ttaaatcctt tagcctagtc agctttgaag
     8881 gtcattaatg gggaacaagc ggtggctttt tgctaaaatt ttgcaaaatt tttcggaaag
     8941 atgaccgctt gtgatttcca aattgaatag aaaaatcaat gagttatcaa gttttagccc
     9001 gtaaatggag accgcaacgc ttctcggaag tagttggtca gcaacacgtt ttatctgctc
     9061 ttgaaaacag cttaagagaa gatagactgc accacgctta cctgttttca ggcactcgtg
     9121 gtgtgggtaa aacctctatt gcccgtttat tcgccaaagg cttaaactgt gtaaacggga
     9181 tcaccgccga gccttgcggt gaatgtgcga attgtaaagc gattgaagaa gggcatttta
     9241 tcgatctcat tgaaattgat gctgcttctc gtactaaagt ggaagacacc cgtgagctac
     9301 ttgataacgt tcaatacaaa cccaccgttg ggcgttttaa ggtttattta attgacgaag
     9361 tgcatatgct ctctcgtcat agtttcaacg ccttactaaa aacccttgaa gagccgcctg
     9421 aatatgtcaa attcttgctg gctaccactg atccgcaaaa actgccgatt acgatcctct
     9481 ctcgctgtat gcaatttcat ctgcgggcat tagagcaatc gcaaattgcc cgtcatcttg
     9541 aatttattct aaatgcagag aacattccgt ttgagccttt ggcactagaa aagttggcaa
     9601 aagcagcaca aggtagtatc cgagactctc tcagcctcac cgatcaagcc attgcaatga
     9661 gtaatgccaa tattacgctt gcgatagtca gccaaatgct agggttgatt gacgataatc
     9721 aaccactaga attagtgcaa gcaaccgccc aagccgacgg tgaaaaagca atggcgatca
     9781 tcgagcaaat tgcagcgaaa ggcgttgatt ggcaacaatt attaagcgat gtggcagaag
     9841 tgttacacaa aattgcaatg ttgcaattag tgaaaacacc tacaaccgaa gaaacattag
     9901 tgcattttct ggcaaaacaa attccgcccg aagacgtgca atttttctat caattaatcc
     9961 tcaacagtaa aaaagagttg ccttttgcac cggagcaacg tgccggtgtt gaaatggcga
    10021 ttttgcgtgc gttggctttc caccctaaac aggcagaaaa aatagcaaca gttgcagtgg
    10081 aaaaggttac acaaccaaac cattcggttt cagcggttac gacagcaaaa ccacaaaatc
    10141 aaattagcca gctaagagaa aattttgagc aaaatcagca aaaaacggtt actgttgcca
    10201 atactaccac agcggttgaa agcattgctc aaccgccaca ggcagtagat accgctattt
    10261 cgccgtctgc tcctgcgact ctttcaccgg caatggcagc cctcgaagcc cgcaaaaagc
    10321 tacaagcaca gcaggcaaat ttagctcaaa ccgaaaaaaa aaagcctgag ctaaaagccc
    10381 ctcaagttat tgcaaaaaaa tcggcaaatc agaccgcttc attgcaagag cggtttatca
    10441 gcttggcgga aaatcagcac aaagccgtac tgcaagtttc aaagaaaacc gaatccgccc
    10501 ctaaaagtgt ggaagatgaa gattatcgct ggacgtggct caatcctgaa ttagaaaatc
    10561 aagcagacag tgccaaacct tctgatatta aacaagcgat tttgcaagaa cgcacccctg
    10621 aattagtggc aaaaaccatt catttatctt gtcagcagga taaatggtgt gacatcgcca
    10681 actcactcaa tttaggcgga ttttcccgtc agatcgcctt aaatagctat ttagcggaac
    10741 aaaatgaaca gcacctcgca ttggtgttaa agccgaatat ggctcactta gataatccgg
    10801 aatcacgtaa aaatttagaa gctgcccttg ccgaaaaagg ctattcatac gaattgacca
    10861 ttggtgacaa tagcgaacag caaactccgc ttgaaattcg gcgagccatt ttcaaacaat
    10921 taactgaggc agcaaaatca gctctgctta acgatgaaaa attaatgtta cttcgcaacg
    10981 cttttgatgc acaaattgat gaatcgacca ttcgagcggt ggcggaaact gagtaaaaca
    11041 aatgaaagca ttagaattaa ttgatttggt aaaaacctac ccaacaggtg taaaagccgt
    11101 taaaggcatt aatcttgtgg tcgagcaagg tgatttctat gccctacttg ggcataacgg
    11161 ggcaggtaaa tctactacta ttggcattat cagctcactg gtaaataaaa ccagcggcag
    11221 cgtgaaagtg tttggctatg atttagacac ccaaaaagtc gaattaaagc atcaaatcgg
    11281 cttagtgcca caagagttta atttcaatca gtttgaaaaa gtgattgatg tgctagtgca
    11341 acaagccggg ttttacggca ttcctcgcag tgaagcctta gtacgtgccg aaaagtggct
    11401 gaaattgctc gacctatggg aaaaacgcac ccacctcacc cgtgagcttt ctggtggaat
    11461 gaaacgtcgg gtgatgattg cccgagcctt aatgcacaat ccaaaactac tgattttaga
    11521 tgaacctacc gcaggggtgg atattgaact ccgccgctca ttgtgggatt tcttgcggga
    11581 tctaaacaat caaggcacaa ctattattct cactacccac tatttagaag aggcggaaac
    11641 cctatgccgt cacatcggca ttattcaaca cggcgaatta attgaaaaca cctcaatgaa
    11701 agccttgctt gccaaattgg aaagtgaggt atttgtgtta gatttgcaaa attctgagca
    11761 aaatcaaccg cttgtgattc aaaattaccc gcataaatgg cttgatgaaa acacccttga
    11821 agtgaatgta aaacgccagc aagggctaac caatctgttt tcgcaaattt ccgcccaagg
    11881 ggctgaggtg ctaagtatgc gaaacaaatc aaaccgcttg gaagaactat ttatcaatat
    11941 ggctcagtaa aatgaattta atcggatttt ataccttagc ttacaaagag tgccgccgca
    12001 ttttgcggat ttggcgacaa actttagtgc caccggtgat tactaccacg ctctattttt
    12061 taatcttcgg cacgctgatt ggtgggcgaa tcggggaaat gaatggcgtg ggttacaccc
    12121 aatttattgc acccggctta attatgatga ccgccatcac cgcctcttat gtgaataccg
    12181 cctcttcatt tttcttgagt aaatttaccc gagattttga agagatgctg atttcgccac
    12241 tctctaccca taatattatt tggggttatg tcaccggcag tattttgcgt gcaggtttag
    12301 ccgcaacctt agtaatgtgc gtggcaatgg tgtttgtctc gtttgaaatc tactcggttg
    12361 cgattatcgt cttaaccctg ttgctgacaa tcattgcctt tgcgttaggt ggcgtaatta
    12421 atgcggtgtt cgcccgctcc tttgatgatg tggggctgat tccaaccttt gtgcttactc
    12481 ccttaactta cttaggcggc gtattttact ctatttcatt actgcctgaa ttttggcaaa
    12541 cggtgtctaa attcaacccg atcgtttata tgattaacgg cttaagatat ggctttctcg
    12601 gtatcagcga tgtgccgtta ggttatactt ttgcagtgct tacttcactt tgcatcggct
    12661 tgtatattat tgcctattcc ttaattcaac gaggggttgg gctgcgttct taactaattg
    12721 tgaggttatg aaaatgaaag cattaaaaaa actagcaatt acaatggcat taacgttatc
    12781 aggcacagcc ttttcacaag agattaccgt gtttgctgcc gcttcaatga caaatgtgtt
    12841 acaagatatt cagcaggatt ttgaaaaagc ctatccacag gataaaatca cgttctcctt
    12901 cgcttcatca tcggtattag caaagcagat tgagcaagat gcaccggcag atatttttat
    12961 ttccgccgac caaaagtgga tggattatgt tgccgaaaaa caacctgaga aagttaaaca
    13021 tcgccgcaat ttagtcagaa acgagttagt attaattgca cctaccgaaa gtaacattga
    13081 gccaaaagcg atacaagcgg tcgattttgc caaagaatta gcaaacagct atttatcggt
    13141 gggagaccca aaccacgttc ctgccggcaa atatgccaaa aaagccctta gtcattacca
    13201 attatgggaa agtgttgaac ctcgtttagc gagggcaaaa aatgtacgag atgccctctc
    13261 ttttgtcgag cgaggcgaat ccccacttgg gattgtctat tccaccgatg ctaaagtgtc
    13321 gaataaagtc aaagtggttg ccgttttccc accggaaagc tacggcgaaa ttgtttaccc
    13381 tgctgcggca gtcagtgaaa aagcctctgc cgccacattt ttagattttc tcaaaacccc
    13441 gaaagcaaaa gcgaaatttg aggcagctgg gttttatcct gtaaattaaa tttactctct
    13501 ccccttgtgg gagagagaca gaaaaatatg tcgttcagac atatttttca gagagagggc
    13561 aaatagcgtt taaaatattc cccctctccc tgatttttct tctgaacgaa gaaaaatcta
    13621 tccctctccc acaaggggcg agggtaagct tataaccaaa tagttttaga ttatgcttaa
    13681 tttcacccca caagaactca atgcgattgt tctaagccta aaagttgcct ccattgctgt
    13741 agtgcttgca ttaccgcttg ccgtgtgggt ggcgtggctg cttgcccgca aacagttttg
    13801 gggaaaaaat ctgctcaatg gtattatgca tctccctctg gtgttaccgc cggttgtcat
    13861 tggctattta ttgcttattt cgatggcgaa acgaggggta atcgggcaat atttgtggga
    13921 atggtttcat ttcagcttga gtttttcgtg gaaaggagca gtgcttgcct caatggtgat
    13981 ggcgtttccg ctaatggtgc gttctatccg tttagccttt gaggcagttg atcctaaatt
    14041 ggagcaagcc gccagaacgc ttggagcaat accgttaaaa gtgtttttca cgctcacttt
    14101 accgctctct ttttcaggca ttattgccgg tggcgtgctg ggttttgccc gctctttggg
    14161 ggagtttggg gcaaccatca cctttgtctc caacattccc aaccaaaccc aaaccattcc
    14221 tgccgccctt ttcaccttta ttgaaacccc cgatggcgaa atggctgccg cccgtttgtg
    14281 cttggtggcg attctgattt cactcattgc cctgttctgt tcggaatggc tggcagaaag
    14341 gcaaaaacgc tatgctcaaa cttaaacttc ttcagcaact cggcaatctg acgctcaatg
    14401 tcaatctcga tttgccgaat aaaggaatca ccgctatttt cgggcgttcc ggtgccggta
    14461 aatccagcct gattaattta attgcggggc tttcaaccgc tcaagccggc tttatcgaat
    14521 taaacggtca ggtgctgttt gacagccaaa acggcatcaa tttaccacct gaaaaacgca
    14581 acattggcta tgtgtttcaa gagcatcgcc ttttccctca ttattcggtg gaaaaaaact
    14641 taaaatatgg ctacaagcgg tcggattctg cggatttttt gcaaattgtg gaactgttag
    14701 gcattgcccc tttgcttaat cgctatccgg caagcctttc cggtggcgaa aaacaacgtg
    14761 tggcgattgg gcgggcgtta ttaagctcac cgaatatttt gctgatggac gaaccattat
    14821 ctgccctcga tctccctcgt aagcaagagc tactggacta tttagataaa ttatccgctc
    14881 ggcttgaaat accgattctg tatgtcagcc acagtttaga ggaagtagtt cgattggcag
    14941 atcatcttgt gctgttggaa aacggcaaag taaccgcatt tgataccctt tcagcggttt
    15001 ggcacagctc ggcattttcc gcttggcagc caaatcatca gcgaattagc ctgcttgaat
    15061 taccggtgga agaaacactt ccccactata atatgcttgg cttgagcttg aacgcacagc
    15121 gattatgggt gaaccaactc ccccgccatc aaataggcga gaagctaagg gtaactattg
    15181 ccagcaaaga ggtttcaatc agcttacaaa aaccgactca aagctcaatt cgtaatgtgt
    15241 taaaaggcaa tgttcggaaa atagaaacgc atttagatcg ggttgatctg gcaattcaag
    15301 tcgaaaatca gcaaatttgg agtacgatca gcctttggtc ttttaatgaa ttagcgattc
    15361 aaccggagca agaggtctat cttcaaatca aaagcgtttc tttataggct tgcaaattta
    15421 cgctaaaata tgaccgcttg ttaattccca aaagagacat tatgccaatc gttaatcaat
    15481 ctgccctcgt gccttatagt gccgagcaaa tgtatcaact tgttaatcag tatgaaaaat
    15541 accccgaatt tttatccggc tgcgtgagta ccaaaaccct tagcaccggc gaaaatgaat
    15601 taaaggcaga gttacacatt cagaaattag gcattagcca aactttcagt acccacaata
    15661 aaatgacgcc gccctataaa atcgagatgg cattaattga agggcctttc cgccatttac
    15721 acggagcttg gacattcacc ccctttgatg agcaaagttg caaaattgcc ctgcaattaa
    15781 attttgaatt ttccagccca attgtagcaa tggtgttcgg caaggtgttc aacgaattaa
    15841 ctttaaaaat ggtaaatgct ttcaaacaac gtgcaaaaga ggtgtatggt gtctgaacaa
    15901 cgtcaaaatc cagaagaaca aaaaagtgaa gaacagaaaa ttaatgttga ggtgctttat
    15961 gcttaccctg ataaatattt tctgaaaaaa ctgcaagtac cgcaaaacgc gaccattcaa
    16021 actattattt tgctctccgg cgtgttggaa aaatataccg aaattgactt gcgtgaaaac
    16081 aaagtcggta tttttagccg cccggcaaaa ctgaatgaaa cggtggaaga aggcgacaga
    16141 attgagatct atcgcccgtt aattgctgat ccgaaagaaa tcagacgcaa aagagcggct
    16201 gagcaagcgg agaagaaata gctttctcct attcaatcta ggggtgaata attattcgcc
    16261 ctatctattt tctccctcgc cattcctgct ataaattccc tcctttgatt aaatatgaaa
    16321 atctttcata aattttatgt aaattttgag tttgattata tttaatcaac tttctatagt
    16381 tcactctaaa taaaatttta tcaattttga tgcagaggga gcttatggat attcgtcaaa
    16441 aaattgctca taacaaaatg acttcattcc aatggttagt ggttgggctt acggtgttac
    16501 ttaatatgct ggacggcttt gatttaatgg cactcgcctt taccgccaaa agcatccgag
    16561 aagaattgca gttagacggt gcagccatcg gcagcttaat gagtgccggt ttattcggta
    16621 tggcgatagg ctcactcttt ctttcaccac ttgctgataa atttggtcgc cgcccgttgc
    16681 ttttaatcgg cacaggctta gccacgttag gaatgctcct aacttatttc gctacttctg
    16741 cgtttgaaat tggtgtatgg cgtgttttaa ccggtttagg cgtagccgcc attttaaccg
    16801 gcactaatgt gctagtgagt gaatactcca gccaaaaatg gcgtggctta gcgattgcaa
    16861 tttatgcctc aggctttggg attggtgcaa tgctgggcgg aatgtctgcg gtgttattgc
    16921 aaggagaata tggctggcgt gcggttttcc tcgttgggtc gattttaacc ggtgcggtat
    16981 tcgttttatt attctttgta ttgcccgaat cggtggaatt tttaatttca aaacacccgg
    17041 tgaatgctaa agctcgttta aatcaaattg ccgttaaatt aggcttagga aacggctggg
    17101 aacttcctga aattgaggca aaaagtaaaa gcaaagtggc aattacccgt ttattcgagc
    17161 ctgaatatcg ccgcaatacg ttgctaattt ggcttgcctt tattgcaatt tcagcctcat
    17221 tttactttat cagctcttgg actccggcac tattagaaga agccggaatg cctaaaaccc
    17281 aaagccaaac tgtgggaatg gcaatttcag ttggtggtac aatcggctcg ttaattttcg
    17341 gctttttagt cagtcgctgg gcggcaaaac atattttgca ggcattcagt attctggctg
    17401 catctgctgt ggttgccttt gtgtttgccg gcaatttagg gcttgcgata acactagcca
    17461 ttttggttgg cggattaatt aacggctgca ttaccgggct ttataccatt aacccaaccc
    17521 tatacgcacc ggatttccgc agcacgggcg taggcaccgc catcggtgta gggcgtttag
    17581 gctcaattgt ttccccaact cttgcaggta ttttattaga cgccggctgg aaaaagaacg
    17641 agctttattt aggtgcagca gtcgtcttat taattgctgc tttcacggta cattttttaa
    17701 aacagagaaa ataagagcta ccccgtaatt aattgaggtt ttattcaatt aattacgggg
    17761 caagattata taattttttt gattaaattt atttctcagg tgcttgttca agtagtttgg
    17821 ttaggagttt ccaataaatc tccactgccg gaatatgcac tttttcgtcc ggagaatggg
    17881 cgttgagaat agttggtcca atcgagacaa aatccatatt cgggtagatt ttgttgatta
    17941 agccacattc tagccctgcg tgaatcactt taatttgaga ttcataacct aaaatttcat
    18001 cataaatcgc tttggttaat ggcgtgattt tggaatttgg gtttggctcc cagcccgggt
    18061 agttgcccga aaattccact tctgctccca ctaatgcggt taatgagcgg attttactgc
    18121 gaatttcctc ttttccgccc tcaattaatg agcgggcaag aatggtcgct ttcacctcac
    18181 tcgcttcggt gttaagcaca ccaatactga gtgaggtttc caccacattt tccaccacat
    18241 cgctattacg caccacacca tttggcatca catttaagaa atggattact cgagcagtgc
    18301 tggcaaggtc aaaggcttgc tcaggcagtt gagcttcttc aattaaaaat tggaagtttg
    18361 gctcggcaat tttgagttct gcttttactt gttcggcggt ttggcttaaa caagcggtta
    18421 atttttgctg attttttgca gagaaaacca aggttgcgtg ggcttctcgt ggaatggcat
    18481 tccgcaccga accgccttga atttctgcaa tttggaattc cacttcgttg tgggcttgtt
    18541 ccaaaatgcg ggcgaacact ttaatcgcat tcgctcgggt ggtgtgaata tcacagcccg
    18601 aatgcccgcc gtttaggcct ttaagcgtaa cggttactgc actatcttgc tgatgaggca
    18661 ccaagaaaat tggcagtgtc acattcacat tttccccacc tgcacagccg atgtaaattt
    18721 ccccgttttc ttcggtgtcg gtgttaatca taatatcggc ggttagccag ttcgggcgta
    18781 aaccaatcgc cccttccata ccggcttctt cggtcattgt gagcaacact tcaatggttg
    18841 ggtgggctaa atcatcgcta tccaacaccg ccaagcacga agccaatcca atgccgttat
    18901 ctgcccccaa tgttgtgcct ttggctttta cccattcgcc gtcaatataa ggctgaatcg
    18961 gatctttagt gaaatcgtga accgtgccgg tattggcttg tggcaccata tcaagatggg
    19021 cttgcagagc gacagtttgg cggttttcca tacctttagt tgccggttta cggattaaca
    19081 cattacccac ttcatcacgt tcaacaaaga aatttttcga ttttgcccaa ttcacaataa
    19141 attcagccag ttcattttcg tggtaagacg ggtgcggaat agcacaggct ttatcaaacc
    19201 actgccaaag tagctgcggt gcgagcgttg agatttcaga cataataagc cccttttgta
    19261 aaatttta
//
LOCUS       DANLXF010000017        19194 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.017, whole genome shotgun sequence.
ACCESSION   DANLXF010000017 DANLXF010000000
VERSION     DANLXF010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 19194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19194
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.017"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            7..480
                     /gene="nrdG"
                     /locus_tag="PXD66_000359"
     CDS             7..480
                     /gene="nrdG"
                     /locus_tag="PXD66_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDL6318694.1"
                     /translation="MENLRFSSEQIVWQEVPNETSLAFLITGCPLGCKGCHSADSWKA
                     CLGKELSSEYLSERLKRYQGLISCVLFMGGEWEAEKLLPLLQLAKNQGLNTCLYSGLE
                     QHEIAETLLHELTYLKTGRWIAERGGLESLTTNQRFTDLRNNQNLNHLFIRGASI"
     gene            477..2258
                     /gene="nrdD"
                     /locus_tag="PXD66_000360"
     CDS             477..2258
                     /gene="nrdD"
                     /locus_tag="PXD66_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDL6318695.1"
                     /translation="MIRLQPTQLDGKLHFINQYIQAKNAADGSKMDANANVTQKNIAT
                     MEQELMKDFFVQINRAKVSEKIAKIFGKETACEYLRQIEAHEIYVHDETSLKPYCVSV
                     TMYPFLRDGLTKLGGESKAPKHLASFCGSFINFVFAVSSQFAGAVATVEFLTYFDYFA
                     RKDYGDNYLQTHRSDIENHLQQVVYSINQPAAARGYQSVFWNISVYDKFYFEAMFSRF
                     VFPDFEKPNWETTSKLQLFFMQWFNKERSKAILTFPVVTAAMLTEKGKCKDDEFANQM
                     AKELSQGNSFFIYQSDNPDSLASCCRLRNEISDHSFSYSLGAGGVATGSINVITINMN
                     RLVQDNRDLSEEVSKIHKYQYAYRKLMEEYLAAGMLPVYDAGFISLHKQFLTIGINGM
                     AEAAESQGLTVGYNPDYIDFVQSRLKIIFQANQAAGEKYGVKFNTEFVPAENLGVKNA
                     KWDKEDGYFVPRDCYNSYFYVVEDEEINALDKFLLHGRELIEWLDGGSALHLNLDEAL
                     TQTGYRSLLDTAAKTGCNYFCINVKITICNECSHIDKRTLHSCSKCGSDNIDYGTRVI
                     GYLKRVSAFSSARQKEHSLRFYHREAA"
     regulatory      2315..2411
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2468..3286
                     /gene="thiM"
                     /locus_tag="PXD66_000361"
     CDS             2468..3286
                     /gene="thiM"
                     /locus_tag="PXD66_000361"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDL6318696.1"
                     /translation="MTTSLPYHSHYLQKIRQNRPLVHNITNIVAANFSANGLLALGAS
                     PIMAAAIEEMESVPALSNAVVINIGTLIGKEVEAMELAGKTANRLGIPLILDPVGVGA
                     TPFRRQTVETLVDQIHFTAIRGNAGELATLAGVEWQAKGVDAGEGSHNVVEIAQQIAK
                     RYQTLVAISGETDVVSSGSHTALLHNGSPMFPKITASGCLLSAVCGAFLAVADKEHYF
                     NALIEACAAYAVAGEVAAQALKPTQYGQFAVNLLDTLGALTPETVEQYVRVEYV"
     gene            3279..4592
                     /locus_tag="PXD66_000362"
     CDS             3279..4592
                     /locus_tag="PXD66_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL6318697.1"
                     /translation="MSKTHSNPTKVAVASMVGTAIEYFDNYIYTMAAVLVFNVQFFNS
                     DDPVSNQLMSLSVLALAFFTRPMGSILFGHFGDKYGRKQTLIASLLLMGLSTVAIGLL
                     PNYAAIGIWATVLLCLCRIGQGLGLGGEWGGAALVATEHAPKHKRAFFGIFPQMGAPI
                     GLLLANGAFYLVNLIYGEQTFLDWAWRIPFVASIVMIAIGLYIRIKISESPIFLKAEQ
                     QGKNRHTPLKTLFSQHLKPFMIGVLIATAGYVLFYILVAFTPIFVKSPVAVSQAGHAT
                     GLGLPVNLYTQYLLITSVIFGIAIFFSGIYSDKIGRRLLLLLATGATVVYGLTMPFFL
                     ENGTPTSIFIFLTIGMILIGLSFGPMAVILPELFPTEVRYTGASLAYTAAGILGASVF
                     TIIAVKINENFGLFGVGGYLSVASLLSFWALWQVHETKDLELEEI"
     regulatory      4728..4824
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4892..6421
                     /gene="thiE"
                     /locus_tag="PXD66_000363"
     CDS             4892..6421
                     /gene="thiE"
                     /locus_tag="PXD66_000363"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDL6318698.1"
                     /translation="MSNYSNSPYQPNVIWTVAGSDSCAGAGLQTDLHTFHDFNLVGCS
                     VVTSVTAQHPHGVLCVTPVDDHTFRQQFEALLVQGYPNAIKIGLLCSQAQVEILCEYI
                     QKIRAESSHYCYVVYDPVAVASSGQALSDSILLQIVQQKLYPLVDLITPNGTELALLS
                     DTEIQTFGDVKTASHKLFAQGIKAVLAKGGHFEWLGETVRDYLLTANEIYAFSHARQQ
                     SVNTHGTGCTYASAVGAMLATGFDLADAVTVATAYLQKGLSEKQGRGQTALSSLCHTG
                     YPDDIAFFPQTELISAPLKLPKEPFAPTEYQLGLYPVVDSVKWLERLILVGVKTLQIR
                     IKNRPLAEIEQEIAHSVALAKQHNVRLFVNDYWQLAMKYQAYGVHLGQEDLATADLNA
                     IQQSGLRLGVSTHCYFEIMRALAVKPSYIAFGHVFPTQTKVMPSQPQGLTNLAKYVAL
                     VAPLNIPTVAIGGINEQSIENVMHTGVGSAAVVSAVTQAKDWQQAVKNLAKFANGGRN
                     E"
     gene            6414..7586
                     /gene="cytX"
                     /locus_tag="PXD66_000364"
     CDS             6414..7586
                     /gene="cytX"
                     /locus_tag="PXD66_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HDL6318699.1"
                     /translation="MNRTLSTLNSGLIWAGAGISASAIVVGTWIAPLGWQQGLLAIVL
                     GHLLGGILFFAAGLIGAKTGKNAMQTVQISFGRGSVFFSLANVLQLVGWTAIMIYTGA
                     EISNALSLALWEYSAFSLWAIVLGLLIVLWLFTGSNQVGKFKLVTLIAMFLLTLWLSI
                     KVLNGQENFPVSGEMPFSTAVELATIMPLSWLPLVSDYTAKAKKPFAATLSATIGYLI
                     TSAWMYALGLGMVLFTGQTEIAPMLLMTGMSLIGIMVMILSTVTTTFLDAYSAGVSAG
                     NITAKFKETPTAILVTIIGTLLAISLPVTQYENFLLLIGSVFAPMIAVQIADFFVLKR
                     HQAQPNVDWVAAGLWLVGFVLYHLLSYHQVHLLLGLTIPVMLVIFLSTIFVRRFTK"
     gene            7625..7834
                     /locus_tag="PXD66_000365"
     CDS             7625..7834
                     /locus_tag="PXD66_000365"
                     /inference="COORDINATES: protein motif:HMM:NF015737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDL6318700.1"
                     /translation="MESLVLNNDWVVTPQAQLAYHTINGKADEERLNLLVARAGVRVA
                     KGFALNNGWNLQPYAEVNGIAEKGQ"
     gene            7842..8018
                     /locus_tag="PXD66_000366"
     CDS             7842..8018
                     /locus_tag="PXD66_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDL6318701.1"
                     /translation="MQVNQYQFGVAENKGRVQGSLGLSAGNGNHRLGLEASVASGKKL
                     KQPFNVLVNYRYQW"
     gene            complement(8059..9915)
                     /gene="sppA"
                     /locus_tag="PXD66_000367"
     CDS             complement(8059..9915)
                     /gene="sppA"
                     /locus_tag="PXD66_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDL6318702.1"
                     /translation="MLTILKAFYRIFRCIRECVINLFFILFILLLVPVVAFISSSGST
                     QKPVFNQGALRLNLDGYLADNREEFTDFYRLVQSELGSSEPFKISTFDVVQAISHAKN
                     DPKITGLVLDLQKLQSADFSSLDFIGNEINNFKQSGKPVIAVGEDYSQKQYYLASFAD
                     EIYLNKVGAVDLKGLNYSNTYFKTLLDKIEAEPHIFRVGTYKSAVEPFMRDDMSEEAK
                     QNARGWLNGTWQQTVATLAQNRNIEPQALDLSPANYIEKYKQAKGDDAQLALNQKWVS
                     QLVSSQESRDKLIAQFGKNSEGSYNQIEFLDYMTELNDRFAEVNAPKIAVVNVEGAIA
                     GGESDEMNVGSETLVKLLQQARDDKNVEGLILRINSPGGSAVASELIRQEVEAFQQAG
                     KPVVASMGGMAASGGYWIAATSDKIIASPNTLTGSIGIFGLAVTFEKTAKNLGVSEDG
                     IATSALAQQAGLKSLPQEQAEVLQIGIENGYDRFLELVSRGRKMPKEVVDKVAQGQVW
                     LGTDALKHGLVDELGNFNVAYNALSELINQKREAENQPKVEQFGLQWFAEQDDSLFGT
                     LARDFKAQLQFSVANWLDLPLLKQPQQQLETLSRFNDPKQMYLYCLNCGKID"
     gene            10018..10581
                     /locus_tag="PXD66_000368"
     CDS             10018..10581
                     /locus_tag="PXD66_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL6318703.1"
                     /translation="MQILDLLHHRRSNKKFGDKAPNAEQLEQILKAGLRVPDHGRLKP
                     YRFVVIEKSGMPQFGECLKSAVAEFDMGEERLKKAEKLANQAPMIIGVVATLDDSIAK
                     VPSWEQMITAGCATYAIQLAANAQGFDTVWITNKWINGSALRSAFGCQESDKIIALIM
                     LGSPAEGEQIALAPESESLEGFVRYLK"
     gene            complement(10652..12976)
                     /locus_tag="PXD66_000369"
     CDS             complement(10652..12976)
                     /locus_tag="PXD66_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDL6318704.1"
                     /translation="MTDLNLKISQIIAGELNVGSHQILAAITLLGEGNTIPFIARYRK
                     EVTGGLDDTQLRHFETRLIYLREMDERRQTILKSIEEQGKLTDELKAKILSSESKTEL
                     EDLYLPYKPKRRTRGQIAIEAGLEPLADSLWNDPKQDPETLAANFVDAEKGVADTKAA
                     LDGARYILMERFSEDAELLAKLRQYLTAYATLESKVIDGKEEEGEKFRDYFAHSEPFN
                     SVPSHRALAMFRGRNEGVLSLSLNADPTAEEGTKSSHCEEIIREHLGVIFNNQPADKW
                     REQVIAWTWKIKAALHLETELMATLCEKAEEEAIDVFARNLSALLMAAPAGARNTMGL
                     DPGLRTGVKVAVVDNTGKLLDTATIYPHTGREAEAQLAIFSLIKQHNVELIAIGNGTA
                     SRETERFAKAVIKEIKENKPQTVVVSEAGASVYSASEFAANEFPNLDVSLRGAVSIAR
                     RLQDPLAELVKIEPKAIGVGQYQHDVNQSQLARKLDAVVEDCVNAVGVDLNTASAPLL
                     ARVAGMTKTLAQNIVAFRDENGRFTSRSELKKVPRLGPKAFEQCAGFMRIIGGKNALD
                     ASSVHPEAYPVVEKILQATTSTLAELMGNNSKIHSLNAKDFVDEQFGLPTVNDIFKEL
                     EKPGRDPRGEFKTAVFMDGVEEITDLKAGMILEGTVTNVANFGAFVDIGVHQDGLVHI
                     SMLSNSFVEDPHTVVKAGDVVKVKVLEVDAARKRIALTMRLDDKPTDKKEPTSGRNSA
                     EICKNTQKNDRLQSNNRNAFGNNAFADALKGWKK"
     gene            complement(12976..13530)
                     /gene="pilW"
                     /locus_tag="PXD66_000370"
     CDS             complement(12976..13530)
                     /gene="pilW"
                     /locus_tag="PXD66_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HDL6318705.1"
                     /translation="MFAKFFPKMTACILIFLLAACSSLPPSSSEVEFNRSEAVKARIN
                     LALAYLEQNDFPKAKQNIDKALAHDEKDYLPYSVLAYYYQQTGDAENAEKAYQTALKL
                     SQNRPDVLNNYGTFLCKQGQFQQAYQQFEQAVKSKAPYYHQADSLENMVLCAKKEPNL
                     QKIAETLTELEKLDKPRANLLKEN"
     gene            13750..14001
                     /locus_tag="PXD66_000371"
     CDS             13750..14001
                     /locus_tag="PXD66_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL6318706.1"
                     /translation="MKIVVFQRKALKQLRKIPTGAEIRRKCDDLAYFPYCANIKALTN
                     HQYDYRFRVGNYRIFFNIEGETMNIVSIEEVKKRDERTY"
     gene            13985..14332
                     /locus_tag="PXD66_000372"
     CDS             13985..14332
                     /locus_tag="PXD66_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015702293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL6318707.1"
                     /translation="MKELTNIQYLNNEEGKPAFAVLPIATLEWLKQKANIKTAIETGI
                     PSAVAGIALDNNYSALRAWREYLGLTQEEVAQRLGISQSAYSQHENAKNLRKTTRIKI
                     AYALGLNEEQLDF"
     gene            complement(14373..15533)
                     /gene="trmG/rlmN"
                     /locus_tag="PXD66_000373"
     CDS             complement(14373..15533)
                     /gene="trmG/rlmN"
                     /locus_tag="PXD66_000373"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HDL6318708.1"
                     /translation="MSELITNAGCGSAEQPKEKINLLNLNRQEMRELFAEMGEKPFRA
                     DQLMKWIYHFGEENFDNMSNINKVLREKLKRIAEIKAPEVAVEQRSADGTIKWAMQVG
                     DQQIETVYIPEDDRATLCVSSQVGCALACTFCSTAQQGFNRNLSVAEIIGQVWRASKI
                     IGNFGVTGVRPITNVVMMGMGEPLLNMNNVIPAMEIMLDDFAYGLSKRRVTLSTSGVV
                     PALDKMREQIDVALAISLHAPNDELRDELVPINKKYNIKMLMDSVNKYREVSNANHGK
                     VTIEYVMLDHVNDSTNHAHQLAEVLKNTPCKINLIPWNPFPEAPYGKSSNSRVDRFQK
                     TLMEYGFTVTVRKTRGDDIDAACGQLAGDVIDRTKRTLEKRKFGQGIAVKTH"
     gene            15793..16689
                     /locus_tag="PXD66_000374"
     CDS             15793..16689
                     /locus_tag="PXD66_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDL6318709.1"
                     /translation="MKLSTIKKALVLTFGLSAAMAMAEPFKVVTTFTVIQDIAQNVAG
                     DKATVESITKPGAEIHDYQPTPKDIVKAQKADLVLWNGMNLERWFERFFENVKGKPAV
                     VVTEGITPMPITEGEYKNLPNPHAWMSTDNALIYIENIRAALVKYDPQNADTYNANAK
                     AYAEKVKGIAEPLRQRLAFIPEQQRWLVTSEGAFSYLAKDYNLKELYLWAINAEEQGS
                     PKQVKKVIDGVKANKIPVVFSESTVSDKPAKQVAKETGALYGGVIYVDSLSEKDGPVP
                     TYLDLLKVTIGTIVEGFERSKK"
     gene            16702..17445
                     /locus_tag="PXD66_000375"
     CDS             16702..17445
                     /locus_tag="PXD66_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL6318710.1"
                     /translation="MTTVSASISVENLSVRYNNGHIALQDVSFHLENGTICALIGVNG
                     GGKSTLFKSLMGLVKPLTGSVKLSQLPIAKALKQNLVSYVPQSEEVDWQFPVSVYDVV
                     MMGRYGYMNFLRRPSQTDKLKVEQAMQRLDVLHLKDRQIGELSGGQKKRVFLARALAQ
                     ESKIILLDEPFTGVDVKTENAIVELLKQLKNEGHLILVSTHNLASVPSFCDQVLMVNR
                     TLLAKGKTEEIFNNQNLERVFGGLLHYQK"
     gene            complement(17502..18311)
                     /locus_tag="PXD66_000376"
     CDS             complement(17502..18311)
                     /locus_tag="PXD66_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL6318711.1"
                     /translation="MLNWILEPLQFEFMQIALITAILVAGVSAVLSCYLVLKGWSLMG
                     DAISHAVLPGVVISSLAGLPLSIGAFFSGLGCALSVGYLKENSRLKEDTVMGIVFSGM
                     FAFGILLFTAFNTGEHLTHILFGNLLGVTKSDLLQTLLISLFTFVVMVAKRKDFLLYC
                     FDVNQAKVAGLPVRLLHYGLLSLLALTIISAMQVVGVILVVAMLITPGITAYLLTKRF
                     DSMLLIALGVAIFSSVFGTLLSYHIDAATGPTIILVQAAIFFLSFAYSKIH"
     gene            complement(18304..19164)
                     /locus_tag="PXD66_000377"
     CDS             complement(18304..19164)
                     /locus_tag="PXD66_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL6318712.1"
                     /translation="MLDLLLEPFTYNYMQKAIFVSMGVGLVCAFLSAFLMLKGWSLIG
                     DALSHAVVPGVAIAYALKLPYAFGAFFSGILAALSILWVKSLTKIKEDAVIGFIFTTF
                     FALGMFIVSLNPTSVDVNSIVMGNILGIADDDLWQVVLIIAISLLGLILFWKDLLLVF
                     FDEHHALSVGLSPLRYKVIFFTLLSACVVVALQTVGAILVIAMVVTPGATAYLLTDRF
                     PRLVLIAMLIGTLTSGIGAYLSYFLDGATGAVIVCLQTLIFLFAFCFAPKYGLITQKR
                     KLHRVEVQNA"
BASE COUNT         5488 a         4295 c         4069 g         5342 t
ORIGIN      
        1 ttagtaatgg aaaatctcag attcagctcg gaacaaattg tttggcaaga agtgccgaat
       61 gagacctcac ttgcttttct gattacaggc tgcccgcttg gctgtaaagg ttgccatagt
      121 gcggatagct ggaaagcctg tcttgggaaa gagcttagca gtgaatatct ctctgaacgc
      181 ctaaaacgct accaaggctt aattagctgc gtattgttta tgggcggaga atgggaagca
      241 gaaaaattac tgccgctgtt gcaactggca aaaaaccagg ggctaaacac ctgtctttat
      301 agtggtttgg agcagcacga aatcgccgag actttattac acgagctaac ctacttaaaa
      361 accgggcgtt ggattgccga acgaggagga ttggaaagtt taaccaccaa ccaacgcttt
      421 actgatttac gcaataacca aaatcttaac catcttttta ttcgaggagc ttcaatatga
      481 ttcgcttaca accgacacaa ttagacggaa aactacattt cattaaccaa tatattcaag
      541 ccaaaaatgc cgcagacggt tcaaaaatgg acgccaacgc taacgtcacg caaaaaaaca
      601 tagcgacaat ggaacaggag ctaatgaaag atttttttgt gcaaatcaac cgagcaaaag
      661 tgagtgaaaa aattgcaaaa atttttggaa aagaaaccgc ttgtgaatat ttacgtcaaa
      721 tcgaagcaca cgaaatttac gtacacgatg aaaccagcct aaaaccctac tgcgtttcag
      781 tcactatgta ccctttcttg cgtgatggtt tgactaaact cggcggcgaa tccaaagccc
      841 cgaaacattt agcctcattc tgcggttctt ttatcaattt tgtctttgca gtcagttctc
      901 agtttgccgg tgcagtcgcc acggttgaat ttctgaccta ttttgactat tttgcccgca
      961 aagattacgg cgataattac ttacagactc atcgttcaga tattgaaaac cacctgcagc
     1021 aggttgtgta tagcatcaac caaccggcag ccgctcgtgg ttatcaaagc gtcttttgga
     1081 atatttcagt gtatgacaaa ttctattttg aagcgatgtt tagccgtttt gtttttcctg
     1141 attttgagaa accaaattgg gaaaccactt caaaattaca acttttcttt atgcaatggt
     1201 tcaataaaga gcgaagcaaa gcgattctca ctttccctgt tgtgaccgca gcaatgctta
     1261 ccgaaaaggg caaatgtaaa gatgacgagt ttgccaatca aatggcaaaa gagctatctc
     1321 aaggcaattc attctttatt tatcaatcag ataatccgga ttcccttgct tcttgctgcc
     1381 gcttgagaaa tgaaatcagc gatcatagct tctcctattc attaggggct ggaggtgttg
     1441 cgacaggttc aatcaatgta attactatta atatgaaccg tttggtgcaa gataaccgtg
     1501 atttaagcga agaagtcagc aagatccata aatatcaata cgcttaccgc aaactgatgg
     1561 aagagtattt agctgccggt atgttgccgg tttacgatgc cggttttatt tcactgcata
     1621 aacaattcct gactattggc attaacggaa tggcagaagc tgctgaatca caaggtttaa
     1681 ccgtaggtta caatccagac tatattgatt ttgtgcaatc tcggttaaaa attattttcc
     1741 aagctaacca agcagccggt gaaaagtatg gggtaaaatt taataccgaa tttgtgcctg
     1801 ctgaaaattt aggggtcaaa aacgccaaat gggacaaaga ggacggctat ttcgtaccac
     1861 gagattgcta caattcctat ttctatgtgg tagaagatga agaaattaat gctttggata
     1921 aattcttact gcacggcaga gagctgattg aatggctgga cggaggctcc gctctacact
     1981 tgaatttaga cgaagcctta acccaaaccg gatatcgctc cttgctggat accgccgcta
     2041 aaaccggctg caattatttt tgtattaacg ttaaaatcac gatttgcaac gaatgtagcc
     2101 acatcgacaa acgcacgttg cacagttgta gcaaatgtgg ctccgataat attgattacg
     2161 gcactcgggt aatcggctat ttaaaacgag tctccgcctt tagttccgcc cgccaaaaag
     2221 agcacagctt gcgtttctat caccgagaag cagcttaatt gaaaactgca gttgtagccc
     2281 tttacaaccg cttttttagt gattatatta aagcctcatt tagtcggggt gcttgcctaa
     2341 gggcaagctg agaaataccc gttgaacctg atgcagttag cactgacgta ggaaactaaa
     2401 ggcgtaaacc catctattcc ccgttggtgt tggtgcgttg tttttcaatc atattaagga
     2461 ataaacgatg accacatcac tcccctatca ctcgcattat ttgcaaaaaa ttcgacaaaa
     2521 tcgaccgctt gttcacaaca ttaccaacat tgttgccgct aatttttctg ccaacggctt
     2581 attggctctt ggtgcttctc ccattatggc agctgctatt gaggagatgg aatccgtgcc
     2641 ggcactcagt aacgccgtgg tgattaatat cggcacgctt atcggcaaag aagtagaagc
     2701 aatggagctt gctggcaaaa ccgccaaccg tttaggtatt ccgctcattc ttgaccctgt
     2761 cggcgttgga gcaacacctt tccgccgtca aacagttgaa accttagtcg accaaattca
     2821 cttcaccgcc attcgtggaa atgcggggga actagctacc ctcgccggtg tagaatggca
     2881 agccaaaggc gttgatgccg gcgaaggctc gcataatgtg gttgaaatcg cccaacagat
     2941 tgccaaacgt taccaaacct tagtcgcaat cagtggtgaa accgatgtgg taagctctgg
     3001 ctcacacaca gctttattgc acaacggcag cccgatgttc cccaaaatta ccgcttcagg
     3061 ctgtttatta agtgcggtat gcggggcatt cttagccgtt gccgacaaag agcactattt
     3121 caacgcactt attgaggctt gcgccgctta tgccgttgca ggcgaagtgg ctgctcaagc
     3181 actcaaacca actcaatacg ggcaatttgc cgtcaatctg ctggataccc ttggggcatt
     3241 aacgcctgaa accgttgagc aatatgtgag ggttgaatat gtctaaaact cactcaaacc
     3301 caactaaagt ggcggttgcc tcaatggtcg gcacggcgat tgaatatttt gataactaca
     3361 tctacacgat ggcagcagtg ctggtgttta atgtgcagtt ttttaatagc gatgatccgg
     3421 tttccaacca attaatgtcg ctttccgtac tagctcttgc ctttttcacc cgcccgatgg
     3481 ggtcgatttt attcgggcat ttcggcgata aatacggacg caaacaaact ctgattgcct
     3541 cgctcttatt aatgggctta tctaccgtag caatcgggct tttgcctaat tatgccgcca
     3601 ttggcatttg ggcaaccgtg ttactctgcc tctgtagaat cggacaaggt ttaggcttag
     3661 gcggcgaatg gggcggagct gctctcgttg ccactgaaca cgccccgaaa cacaagcggg
     3721 cattttttgg tatttttccg caaatgggcg ccccaatcgg cttgctgttg gcaaacggag
     3781 cgttctactt ggtgaattta atttacggtg aacaaacctt cttggattgg gcgtggcgaa
     3841 ttccgtttgt cgcttctatt gtgatgatcg ccattgggct ttatatccga attaagatca
     3901 gcgaaagccc gattttcctt aaagcggaac aacaaggtaa aaaccgccat accccgctta
     3961 aaactttgtt tagccaacac ctcaagcctt ttatgatcgg cgtgctgatt gccaccgccg
     4021 gctatgtgtt gttctacatt ttagttgctt tcaccccgat tttcgtcaaa agcccggtgg
     4081 cagtttctca agcaggacac gctacaggct taggtttacc ggtaaatctc tacacccaat
     4141 atttgctgat tacctccgtg attttcggca ttgcgatttt cttctccggc atttattcag
     4201 acaaaatcgg acgccgttta ctgctgttac ttgccacagg agcaaccgta gtttacggct
     4261 taacaatgcc gtttttcctc gaaaacggca caccgacttc gatctttatt ttcctcacca
     4321 ttggaatgat cttaatcggc ttaagcttcg gcccgatggc agtaattcta cccgaactct
     4381 tcccaacaga agtacgttac acaggggctt cgcttgccta caccgctgcc ggtattttag
     4441 gagctagcgt gttcactatt attgcggtca aaatcaatga aaatttcggc ttattcggtg
     4501 taggcggcta tttatcggtt gcctcattac tgagcttctg ggcattgtgg caagtccacg
     4561 aaaccaaaga tttggaatta gaggagatat aaaaaaccac tcacttaacg acggggcaag
     4621 ttaccacctt gccccaagat gagggaatgc aaatttccct ctaatagact tcacaacgga
     4681 tattttgcat ttttttataa aaaataaccg cttgtaagct atactaaaaa cgcttgtcgg
     4741 agtgccgtaa ggctgagatc gcaacagcga gatccgtgga acctgtaggt tagtacctgc
     4801 gtagggaaca agttttaccc aaattcagat tttgggatca ttaccacatc tctggcaagc
     4861 aaaaccctaa gcatattatg aggattttgc gatgtctaat tattcaaact ctccatacca
     4921 acctaacgtc atttggacgg ttgccggctc agattcctgt gccggtgccg gtctgcaaac
     4981 cgatttacat acctttcacg attttaacct agtgggttgt tcagtagtta cttctgtaac
     5041 ggctcaacat ccgcacggtg tgctgtgtgt aacgcctgtt gatgatcaca ccttccgcca
     5101 acagtttgaa gccttgctgg ttcaaggcta cccgaacgca atcaaaatcg gcttactctg
     5161 ctcgcaagca caagtagaga tcttgtgtga atatatccaa aaaatccgtg cagaaagctc
     5221 gcattattgt tatgtggttt acgaccctgt ggcagtggca agcagtgggc aggcgttatc
     5281 cgacagtatt ttattgcaga tagtgcaaca aaaattgtat ccgctggtgg atttaatcac
     5341 tccaaacggc actgaactcg ctttattaag cgacacagaa atccagacct ttggtgatgt
     5401 gaaaaccgct agccacaaac tgtttgcaca gggaatcaaa gccgtgttag ccaaaggtgg
     5461 acattttgaa tggctggggg aaacggtaag agattatctg ctgactgcaa atgaaattta
     5521 tgcctttagt cacgcccgac agcaaagtgt gaatacccat ggcacaggtt gcacctatgc
     5581 ttcagcggtt ggagcgatgt tggcaaccgg ctttgattta gctgatgccg tcactgttgc
     5641 caccgcttat ttgcaaaaag gcttatcgga aaaacaaggc agagggcaaa ctgctttaag
     5701 ttcactttgc cacaccggct accctgatga tatcgctttc ttcccacaaa ccgagctgat
     5761 ttctgcccct cttaaattgc cgaaagagcc ttttgctcca acggaatatc aacttgggct
     5821 ttaccctgtt gtagatagcg ttaaatggct tgaacgctta attcttgtag gggtaaaaac
     5881 cttgcaaatt agaatcaaaa atcgaccgct tgcagagatt gagcaagaaa ttgcccattc
     5941 ggtcgcactg gcgaaacagc acaatgtgcg tttgtttgta aatgattact ggcagttagc
     6001 catgaagtat caggcttacg gcgtgcattt agggcaggag gatttagcca ccgccgattt
     6061 aaatgcaatt cagcaatctg ggttacggct tggtgtttca acccactgct actttgaaat
     6121 aatgcgggca ttggcggtga aaccgtctta tatcgctttt ggccacgttt tccctactca
     6181 aaccaaagta atgccgtctc aaccacaagg cttaacaaat ttagccaaat atgttgctct
     6241 cgttgcaccg cttaacattc caactgtggc gattggtgga attaatgagc aaagcattga
     6301 aaatgtaatg cataccggtg tcggcagtgc cgcggtggta agtgccgtta cccaagcaaa
     6361 agattggcaa caagcggtca aaaacttagc aaaatttgca aatggaggcc gtaatgaata
     6421 gaactcttag tacgctaaac agtggtttga tttgggcggg agcgggcatt agtgccagtg
     6481 caatcgtggt tggcacttgg attgccccac ttggctggca acaaggcttg ctggcgatag
     6541 tgctagggca tttattaggc ggcattttgt tttttgctgc cggcttaatt ggggcgaaaa
     6601 ccggcaaaaa tgcgatgcaa acggtgcaaa tttcctttgg cagaggctcg gtgtttttct
     6661 cccttgccaa tgtgctacag cttgttggtt ggacagccat tatgatctat acaggggcag
     6721 agatcagtaa tgcccttagc cttgccttat gggaatattc ggctttctcg ctttgggcaa
     6781 tcgtgcttgg gttgctgatt gtgctatggt tatttaccgg ctctaatcag gtcggaaaat
     6841 tcaagttagt gacattgata gcgatgtttt tgctcacctt atggctaagc attaaggtat
     6901 tgaatgggca ggaaaatttt cctgtttcgg gtgaaatgcc gtttagcacc gcagtggaat
     6961 tagcaactat tatgcctctt tcgtggctac ctttggtatc agactatacc gctaaagcga
     7021 aaaagccttt tgccgcgacc ctttcggcga ccataggcta tttgatcacc agtgcgtgga
     7081 tgtatgcgtt aggtctgggt atggtgctat ttaccgggca aaccgaaatt gcccctatgt
     7141 tactaatgac aggaatgagc cttatcggca ttatggtgat gattctttcg actgtgacta
     7201 ccacgtttct tgatgcctat tccgccggtg tcagtgccgg caatatcacg gcaaaattca
     7261 aagaaacccc aaccgccatt ctagtcacaa tcatcggcac gctactggca atcagtttac
     7321 cggttaccca atatgaaaat ttcctgctgc tgataggctc ggtattcgcc ccgatgattg
     7381 ccgtgcaaat tgcggatttc tttgtgttaa aacgccacca agcacagcct aatgttgatt
     7441 gggttgctgc cggattatgg cttgtcggct tcgtgctata ccaccttctc tcttaccacc
     7501 aagtgcattt actactcggc ttaaccattc cagtgatgtt agtgattttt ctcagcacca
     7561 tttttgtgag gaggttcaca aaatagaaag ataagcggtt caattggagt gaaaatttgc
     7621 taaaattgag tcgcttgttt taaacaatga ctgggtagta acaccacaag ctcaacttgc
     7681 ctaccacacc attaacggta aagccgatga ggaacggtta aatcttttag ttgcccgtgc
     7741 cggcgtaaga gtagcaaaag gctttgcctt gaataatggt tggaatctgc aaccttatgc
     7801 ggaagtaaac ggcattgctg aaaaaggcca atagtacgaa agtgcaagta aaccaatatc
     7861 agtttggagt tgccgaaaac aagggcagag tgcaaggttc acttggcttg agtgcaggaa
     7921 acggtaatca ccgtttaggt ttagaggcat cagttgcttc aggtaagaaa ctgaaacaac
     7981 catttaatgt attagtaaat tatcgttatc aatggtaatt taagagggca ggtgaaaacc
     8041 tgcccttaaa ttcaataatt aatctatctt cccacaattt aaacagtaaa gatacatctg
     8101 tttcggatcg ttaaaacgtg aaagagtctc tagctgttgt tgcggttgct tcaacagcgg
     8161 taaatcaagc caatttgcca cgctaaattg taattgagct ttgaaatcac gggcaagcgt
     8221 gccaaataag ctgtcatctt gctccgcaaa ccattgtaag ccgaactgtt cgactttagg
     8281 ttggttttcc gcttcacgtt tttggttgat caattcagat aacgcattat atgccacatt
     8341 aaaattgccc aactcatcaa ccaatccgtg ttttaacgca tcagttccca gccacacttg
     8401 cccttgagcc actttatcta ccacctcttt tggcattttc ctgccacgag aaaccagctc
     8461 taaaaaacgg tcgtagccgt tttcaatccc aatttgcagc acttctgcct gctcttgcgg
     8521 taggcttttt aaaccggctt gttgtgctag ggcagaagtc gctatgccgt cttcgctcac
     8581 accgagattt ttagcggttt tttcaaaagt tactgctaaa ccgaaaatac cgatagagcc
     8641 ggtaagcgta ttcgggctag caataatttt atcgctggta gccgcaatcc aataaccgcc
     8701 cgatgctgcc attccgccca ttgaagcaac caccggtttg ccggcttgtt gaaaggcttc
     8761 cacttcttgg cgaatcagtt cagacgccac agcactgccg ccggggctgt taatgcgtag
     8821 aattaagcct tccacatttt tgtcatctcg agcttgttgc agtaatttta ccagcgtctc
     8881 gctgcccaca ttcatttcgt cactttcacc gccagcaatc gccccttcca cattcactac
     8941 cgcaattttc ggagcattca cttctgcaaa gcggtcgttt agttcagtca tataatctaa
     9001 aaattcaatc tggttataac tgccttcgct gtttttgcca aactgtgcaa tcagtttatc
     9061 ccgactttct tggctggaca ctaactgact aacccatttt tgatttaatg ccagttgagc
     9121 atcatctcct ttagcttgtt tatatttttc aatgtaattg gcaggcgata aatccagtgc
     9181 ttgcggttca atgttgcggt tttgagctag tgttgccaca gtttgttgcc aagtgccgtt
     9241 taaccagcct cgggcatttt gtttcgcctc ctcagacata tcatcacgca taaacggctc
     9301 aaccgccgat ttataagtac cgacacggaa aatatgcggt tcagcctcaa ttttatcgag
     9361 caaggttttg aaataagtgt tggaataatt taagcctttt aaatccaccg caccgacttt
     9421 attgagataa atttcatcag caaaggaagc aagataatat tgtttttgcg agtagtcttc
     9481 gcccactgca atcaccggct tgcccgattg tttgaagttg ttgatttcat tgccgataaa
     9541 atccaatgag ctgaaatcag cactttgtag cttttgcaga tctaacacca agccggtaat
     9601 tttcgggtca tttttagcgt ggctaatcgc ttgcaccaca tcaaaagtcg agattttaaa
     9661 cggctcggaa ctacctaatt ccgattgcac caagcggtag aaatcagtga attcttcccg
     9721 attatctgct aaataaccat ctaaatttaa acgtaacgcc ccttgattga acaccggctt
     9781 ttgcgtgctg ccagaagagc tgataaatgc gaccaccggc actaatagca aaataaacag
     9841 aataaaaaag agattaatga cacactctct aatacaacgg aagattcggt aaaatgcttt
     9901 taaaatcgtt aacatcggaa attcctaaaa ataaactcag tcggcacagt ataaagcaat
     9961 cgctaaatga agaaaatatg ctattcttga acgcaaaaaa ttaacaaagg aacgcttatg
    10021 caaatcttgg atttattaca ccatcgccgt tcaaataaaa aatttggcga taaagccccg
    10081 aatgcagaac aactggaaca aattctaaaa gccggcttgc gagtgccgga tcacgggcgg
    10141 ttaaagcctt atcgctttgt ggtgattgaa aaaagcggta tgccacagtt tggtgaatgc
    10201 ttaaagtctg cggtagcaga atttgatatg ggcgaggaac gcttaaaaaa agcggaaaaa
    10261 ttagctaatc aagctccaat gattatcggc gtggtagcaa cattagacga cagcatcgcc
    10321 aaagtgccaa gttgggaaca aatgattacc gcaggctgtg caacttatgc gattcagtta
    10381 gcggcaaatg cacagggctt tgatacggtt tggatcacca ataaatggat taacggctct
    10441 gctcttcgct ccgcttttgg ttgccaagaa tctgataaaa tcattgcctt aattatgctt
    10501 ggctcaccgg cagagggcga gcaaattgca ttagcccctg aaagtgaaag tttagagggg
    10561 tttgtacgtt atcttaagta agcaggttaa cagataaaag ttagtaataa gggcgaaaaa
    10621 tatttcgccc ttgcttttat ctgttttcgc cttacttctt ccagcctttt aacgcatcgg
    10681 caaaggcatt gttaccaaaa gcgttgcgat tgttgctttg caagcggtca tttttctgcg
    10741 tatttttgca aatttctgct gaatttcgac cgcttgttgg ctctttttta tcggttggtt
    10801 tgtcgtctaa acgcatcgta agggcaatac gtttgcgggc agcgtccacc tcaagcactt
    10861 tgactttcac cacatcacca gctttcacta ccgtgtgcgg atcttcaaca aaagagtttg
    10921 agagcattga aatatgcacc aagccatctt ggtgtacgcc aatatcgaca aaagccccaa
    10981 agtttgccac attggtcact gtgccctcca aaatcatacc ggctttgagg tcggtaattt
    11041 cttccacgcc gtccataaac acggcggttt taaactctcc acgagggtca cgccccggtt
    11101 tttctaactc tttgaaaata tcgttcaccg ttggtaaacc aaactgctca tccacaaaat
    11161 ctttggcgtt gagcgagtga attttgctgt tgttacccat taattctgca agtgttgagg
    11221 tagttgcctg taagattttt tctaccaccg ggtaagcttc cgggtgaacg cttgaggcat
    11281 caagggcatt tttcccaccg ataattcgca taaaaccggc acactgttca aaggctttcg
    11341 gccctaaacg aggcactttt ttcagctctg agcggctggt aaaacggccg ttttcgtcac
    11401 ggaatgccac gatattttgt gccaatgttt tggtcattcc cgccacccga gccagcaacg
    11461 gagcggaggc tgtattcaga tccacgccca ctgcgtttac acaatcttct actaccgcat
    11521 cgagtttacg ggcaagttgg gattggttta catcgtgttg atactgccca acaccaatcg
    11581 cttttggttc gattttcacc aactcggcaa gtggatcttg caaacggcgg gcaatcgaaa
    11641 ccgctccccg taatgaaacg tctaaatttg gaaactcgtt agccgcaaat tcagaggcag
    11701 aatagaccga tgccccagcc tcactcacca ccaccgtttg cggtttattc tctttgattt
    11761 ctttaatcac cgctttggca aaacgttcgg tttcacgaga ggccgtaccg ttaccaatcg
    11821 caatcagttc cacattgtgt tgtttgatta agctgaaaat cgcaagctgt gcctccgcct
    11881 ctcgcccagt gtgagggtaa attgtggcgg tatccagcaa tttaccggtg ttatccacca
    11941 ccgccacttt cacgccggta cgcaagcccg ggtctagccc cattgtattt ctcgcaccgg
    12001 caggggctgc cattaataat gccgagagat tgcgggcaaa cacatcaatc gcctcttctt
    12061 cagctttttc acacagggtt gccattaatt cggtttctaa atgcagagct gctttgattt
    12121 tccacgtcca agcaatcact tgctcacgcc atttatctgc tggttgatta ttaaaaatca
    12181 cgcccaaatg ctcacgaata atttcttcac aatggctgga ttttgtgcct tcttctgcag
    12241 tcggatcggc atttaatgaa agtgataaca ccccttcatt tcgcccacgg aacatcgcca
    12301 aagcacggtg ggaaggcacg ctgttaaacg gctcgctatg agcaaaataa tcacggaatt
    12361 tctcgccctc ttcctctttg ccgtcaatca ctttcgattc caacgtggcg taagcggtca
    12421 aatattggcg aagttttgca agtagctcag catcttcgct aaaacgctcc attaaaatat
    12481 atcttgcccc gtctaatgcg gccttggtat ccgcaacgcc tttttccgca tcaacaaagt
    12541 ttgccgctaa ggtttcagga tcttgtttcg gatcattcca taaactgtcc gccaatggct
    12601 ctaaccctgc ttcaatcgca atttgccctc tggtgcgacg ttttggttta tacggcaaat
    12661 agagatcttc cagctcggtc ttgctttcac tgcttagaat tttggctttt aattcatcgg
    12721 ttaatttgcc ttgttcttca atcgacttca aaatggtttg gcggcgttcg tccatttcac
    12781 gcaaataaat taagcgggtt tcaaaatggc gaagttgggt gtcgtctagc ccgccggtca
    12841 cttctttacg gtaacgagca ataaatggaa tggtattgcc ctcacctaat agcgtgatgg
    12901 ctgcaagaat ttggtgtgag ccaacgttga gttcgccggc aataatttgg ctgattttta
    12961 gatttaagtc agtcattagt tttccttcaa taaatttgct cgaggtttat ccaatttctc
    13021 aagctcggtc aatgtttcgg caattttttg cagattcggc tcttttttcg cacataacac
    13081 catattttct aagctatccg cttggtgata gtagggtgct ttgcttttga cagcctgctc
    13141 aaattgctga taagcctgtt gaaattgacc ctgtttacat aaaaaagtgc cgtaattatt
    13201 cagcacatca gggcggtttt gactcaattt cagtgccgtt tggtaggctt tttcggcatt
    13261 ttcagcatca cctgtttgtt ggtaataata ggcaagcacc gaataaggga ggtaatcttt
    13321 ttcatcgtga gccaaggctt tatcaatatt ctgcttagct tttgggaaat cattttgctc
    13381 cagataagct aaagccaaat taatacgggc ttttaccgct tccgagcggt taaattcgac
    13441 ttcagaggaa gaagggggaa gagaagaaca agccgctaat aaaaaaatca gaatacaagc
    13501 ggtcattttt ggaaaaaatt ttgcaaacat aacgttctcc tacaacaaag ggcttaattt
    13561 tactgttcca tcctcgctaa gactagcgat tttttgcgaa gaagaaaaat ttttcgtttt
    13621 catttttcga cgcagatcgc aaaaataata atggggtgtt gtgctagggt aaatggtagg
    13681 aatacactct ctatttaata taagaatttc attgaaaaaa cttataaaac ttataaaata
    13741 gaggcaaaaa tgaaaatagt ggtattccag cgtaaagcct taaagcaatt acgaaaaatc
    13801 ccaactggtg cagaaattcg ccgaaaatgt gatgatttag cttactttcc ctactgtgcc
    13861 aatattaagg cattaacaaa tcatcaatat gattatcgct ttcgggtcgg aaattaccgt
    13921 atttttttca atattgaagg cgaaacgatg aatattgtgt ctatcgaaga ggttaaaaaa
    13981 cgagatgaaa gaacttacta atattcaata tttaaataat gaagaaggaa aacctgcctt
    14041 tgccgtactt ccgatagcaa cacttgaatg gcttaagcag aaagcaaata ttaaaaccgc
    14101 aatagagacc ggtattccat ctgctgttgc tggaattgca ttagataata actactcagc
    14161 tctgcgtgct tggcgggaat atttaggttt aactcaagaa gaggtggcac aacgcttggg
    14221 gatttcgcaa tcagcttata gccaacacga aaatgcaaaa aatctacgaa aaacgacccg
    14281 cataaaaatt gcctatgcgt tgggtttaaa tgaagagcaa ttagatttct aataacaaaa
    14341 aggcaggaac ctctgccttt tcactattca gattaatggg ttttaaccgc aataccttgc
    14401 ccgaatttac gtttttcaag ggtgcgtttg gtgcggtcaa tcacatcgcc agcaagctgt
    14461 ccgcaagcgg catcaatatc atcacctcgt gttttacgca ctgttaccgt gaaaccgtac
    14521 tccatcaagg ttttttggaa acgatctacc cgagagttag agcttttacc ataaggagct
    14581 tctgggaacg ggttccacgg aatcaagtta attttacacg gcgtgttttt taatacttca
    14641 gcaagttggt gggcgtggtt tgtgctgtca ttgacatgat ctaacatcac atattcaatc
    14701 gtgaccttgc cgtggttggc gttagaaact tcacggtatt tattcaccga atccatcaac
    14761 attttgatgt tgtacttttt attaatcggc actaattcat cacgcagttc atcgttcgga
    14821 gcgtgaagtg aaattgccaa cgccacatca atttgctcac gcattttgtc taatgccggc
    14881 accacacccg aagtggaaag cgtgacacga cgtttagata agccgtaagc aaaatcatct
    14941 aacatgattt ccattgccgg aatcacgtta ttcatattca gcaatggttc acccattccc
    15001 atcatgacca cattggtaat cgggcgaaca ccggtcacgc caaaattgcc gataatttta
    15061 gaagcacgcc acacttgccc gataatttcc gccacgctta agttacggtt aaagccttgt
    15121 tgagcggttg agcagaaggt acaagccaag gcacagccta cttgtgaaga aacacacagg
    15181 gttgcacggt catcttcagg aatatatacc gtctcgattt gctgatcgcc cacttgcatt
    15241 gcccatttaa tcgtaccgtc tgcagaacgt tgttctaccg ccacttcagg ggctttaatt
    15301 tcggcaatac gctttaattt ttcacgcaat actttattga tattgctcat attatcgaaa
    15361 ttttcttcgc caaaatggta gatccatttc attagctggt cggcacggaa aggtttttcc
    15421 cccatttctg caaacagttc tcgcatttct tgacgattaa gatttaataa attgattttt
    15481 tcttttggtt gttcagcaga cccacagcct gcattggtaa tcagttctga cataaagttg
    15541 aaagcctcgt tattacacgg attacggcaa aaggttaggg caaatgccct tattttgcga
    15601 ttggatttta aagcgtgcaa ttttactggc tcttgaggga ttaggctagt aatttttaat
    15661 caaaagagtt gaattgcctg agtaaactta tcaagaataa ccttataaaa acaagactat
    15721 ttacatcatt cccaatgttc tatagaatag ctttcgataa ttctcattct caataacaaa
    15781 aggaggctaa aaatgaaact atcaacaatt aaaaaagcat tggtattaac ctttggttta
    15841 agtgcagcga tggcaatggc agagccgttt aaagtagtta ccacttttac cgtgattcaa
    15901 gatattgcac aaaatgtcgc cggcgataaa gccactgtgg aatccattac taaaccgggt
    15961 gctgaaattc acgattatca accaacacca aaagacattg taaaagctca aaaggcagac
    16021 ttagtgttat ggaacggtat gaaccttgag cgttggtttg aacgtttctt tgaaaatgta
    16081 aaaggtaaac cggcggttgt cgtcaccgaa ggcatcaccc caatgccgat taccgaaggt
    16141 gaatataaaa atctaccaaa tccgcacgct tggatgtcca ccgataacgc attaatttac
    16201 attgaaaata ttcgtgcggc tttagtaaaa tatgacccac aaaacgcaga cacctataat
    16261 gccaatgcaa aagcctatgc tgaaaaagta aaaggtattg ctgagccact ccgccaacgt
    16321 ttagctttta ttcctgagca gcaacgttgg ttagtaacca gtgaaggggc atttagctat
    16381 ttagcgaaag actacaactt aaaagaactt tatttatggg caatcaatgc ggaagagcaa
    16441 ggttcgccaa aacaagtgaa aaaagtgatt gatggtgtaa aagccaacaa aatccctgtt
    16501 gtgtttagcg aaagtaccgt ttccgacaaa ccggcaaaac aagtggcgaa agaaaccggt
    16561 gcattatacg gtggcgtaat ttatgtggat tcactttcag agaaagatgg tccggttcca
    16621 acctatttag atttgcttaa agtgactatc ggcaccattg tcgaagggtt cgaacgaagc
    16681 aaaaaataat aagatttagc aatgacaact gtttcagcct ccatttccgt cgaaaattta
    16741 tcggtccgtt ataataacgg acatatcgct ttgcaagatg tctcgtttca tctggaaaac
    16801 ggcacaattt gtgcattaat cggcgtgaat ggaggtggaa aatccactct gtttaaaagt
    16861 ttaatggggc tggttaagcc attaaccggc tctgttaagc tgagtcagct cccgattgct
    16921 aaagccctca aacaaaattt ggtttcctat gtgccacaaa gtgaagaagt ggactggcaa
    16981 tttccagttt cggtttacga tgtggtaatg atggggcgat acggctatat gaattttcta
    17041 cgccgcccaa gccaaaccga taagctcaaa gtagagcaag caatgcaacg ccttgatgtt
    17101 ttacatctca aagacaggca aatcggcgaa ctttccggcg ggcaaaaaaa acgggtattt
    17161 ttagcccgtg ccttagctca agaaagtaaa atcatcttac ttgatgagcc tttcaccggt
    17221 gtggatgtta aaaccgaaaa tgcgattgtt gagctactta aacagcttaa aaatgagggg
    17281 catttgattt tagtttccac ccacaacctt gccagcgtac caagcttttg cgatcaggtt
    17341 ttgatggtca atcgcacgct actggcaaaa ggtaaaacgg aagaaatctt taataatcaa
    17401 aatttagaac gggtgttcgg cggtttattg cattatcaaa agtagtgttt cagcaacaaa
    17461 tcacaaacaa atagggaggc aaatgcctcc ctatttttgt atcaatgaat cttagaatat
    17521 gcaaaactga ggaaaaaaat ggccgcttgc acgagaataa tcgtcggccc tgttgcggca
    17581 tcaatatggt agctaagcaa cgtgccgaat accgatgaaa atatcgcaac gcctaaggca
    17641 atcagtaaca tactatcaaa ccgcttagtc agcaggtaag cagtgatccc cggggtaatc
    17701 aacattgcca ccactaaaat cacgcccacc acttgcattg cactgataat agttaaagca
    17761 agcaaggaaa gtaaaccata atgcaataat ctaaccggta agcctgctac tttggcttga
    17821 ttaacatcaa agcaatagag caagaagtct ttgcgtttcg ccaccatcac cacaaaggta
    17881 aagaggctta tgagtaaagt ctgaagcaaa tcgcttttgg ttacgcctaa tagattacca
    17941 aataaaatat gggttaaatg ctcaccggta ttaaaggcgg taaacagcaa gatcccaaag
    18001 gcaaacatac ctgaaaacac aatccccatt accgtgtctt ctttcaagcg actattttct
    18061 tttaaatagc ccaccgataa ggcacagcct aatcctgaaa aaaacgcccc tattgagagt
    18121 ggtaacccgg caagtgagga aatcaccaca ccgggcagaa cagcgtgcga aattgcatcg
    18181 cccattaaag accagccttt cagcaccaga tagcaagata aaaccgcact cacgccagca
    18241 actaaaattg cggtaattaa cgcaatttgc ataaattcaa attgcaacgg ctctaaaatc
    18301 caattaagca ttttgaacct ccacacggtg caatttccgc ttttgagtaa tcaagccata
    18361 tttaggagca aagcagaatg cgaataagaa aattaaggtt tgtaagcaca caattaccgc
    18421 accggttgca ccgtctaaaa agtagcttaa atatgcccca atccctgatg tcagcgtgcc
    18481 gataagcatt gcgatcagca ctaatctagg gaaacggtcg gtaagtaaat aagcagttgc
    18541 tcccggcgtg acgaccatcg caataaccaa aattgcccca accgtttgca gagccactac
    18601 tacacaggca cttaataagg taaagaaaat cactttatag cgtaatggcg aaagcccgac
    18661 tgacaatgcg tggtgttcgt caaaaaagac gagtagcaaa tccttccaaa ataggattaa
    18721 acccagtaac gaaatcgcaa taatcagtac cacctgccac aagtcatcat cagcaatgcc
    18781 taaaatattt cccattacaa tcgaattaac atcaaccgat gttggattga gggaaacaat
    18841 aaacatacca agcgcaaaaa aagtcgtgaa aataaagccg ataaccgcat cttctttaat
    18901 tttcgtcagg ctttttaccc aaagaatcga taatgccgcc aaaatcccgg aaaaaaacgc
    18961 accaaaggcg tagggcaatt ttagtgcata agcgatggca acacctggca ctacagcgtg
    19021 tgataaggca tcaccaatca aagaccagcc ttttaacatc aaaaaggcgg aaagaaaagc
    19081 acaaactaac cccacgccca tactgacaaa aatcgctttt tgcatatagt tgtaggtaaa
    19141 aggctctaat aaaagatcaa gcataaaatc ctaacaagtt gttgataata gttc
//
LOCUS       DANLXF010000018        19180 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.018, whole genome shotgun sequence.
ACCESSION   DANLXF010000018 DANLXF010000000
VERSION     DANLXF010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 19180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19180
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.018"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(57..2969)
                     /gene="rapA"
                     /locus_tag="PXD66_000378"
     CDS             complement(57..2969)
                     /gene="rapA"
                     /locus_tag="PXD66_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HDL6318713.1"
                     /translation="MFLVGQRWLSESENNLGLGIVTSVDHRTVTINFPAAEEERVYAI
                     AVAPLTRVQFQAGDRINSIDGWQLEVSEVVENQGAVIYLGKRVDNGEEAVLPEMLLDH
                     KISFSKPQDRLFSAQIDRSDRFALRFNALQHQQAQYQSPLRGLRGIRASLIPHQLHIA
                     KEVGQRLAPRVLLADEVGLGKTIEAGMILQQQLFSGRAERVLVLVPESLQHQWLVEML
                     RRFNLKFSLFDEERCSDFDKQDENGDDVSENPFESEALVITSLNWLETSPQRANQVLA
                     ADWDLLIVDEAHHLEWSETESSIGYQLVERLSQQIPAVLLLTATPEQLGQESHFARLA
                     LLDSDRFYDYAEFVQEQAQYKPVADAVSSLLSDKPLSNDEQNAIAELLSEQDTEPMFR
                     AINSKKSDENDRLQARQELISELIDRHGTSRVLFRNTRQGVKGFPHREYHQISLEMPK
                     QYQNALKVMGMMGSVSQSDLLYPEYLFQRMNPNAKWAEFDPRIDWLITFLKNHRDEKV
                     LVICKQADTAIALEQVLREREAIRSAVFHEKMSIVERDKAAAYFAQQEEGAQVLISSS
                     IGSEGRNFQFAKDLVLFNLPDNPDLLEQSIGRLDRIGQKNDIQIYVPCFNLSAQQMLA
                     EWYHKGLNAFEETCPMGTAIFRELGEELQQFLQKPELSAQFDDFVAKTYKRQQYLKAE
                     LEKGQDRLLELNSNGGEAAQQLANDIAKEDNNPQLVNFALSLFDVIGVEQEDLGEQSI
                     VISPTGHMLVPDFPGLSEDGTTITFNRELALMREEVEFLTWDHPMIRNGIDLITSGDI
                     GKSAISLLVNNKLPAGTLLLEALYVVEAQAPKGLNLTRFLPPTPIRVLVDNKGNNIAP
                     QVSFAGLERQLKPVNKQMANKIAKMARADIEKLIKASEKAVEPQVQQVIETAKFEANS
                     KLKAELHRLESLKMVNKNIRADEVEALEQQLNASLAQLETANYRLDSLRVIVSNKA"
     gene            complement(3130..3429)
                     /locus_tag="PXD66_000379"
     CDS             complement(3130..3429)
                     /locus_tag="PXD66_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318714.1"
                     /translation="MRFLPSEIYQPRGELVKADRQGNGEFEVEYRVSGNDVRGLAKSA
                     IAHAKRKGFHLVESDIHRDDADLKFKRGDQELDIEIEVKGRNRIEYKADLDLDKN"
     gene            complement(3550..4875)
                     /locus_tag="PXD66_000380"
     CDS             complement(3550..4875)
                     /locus_tag="PXD66_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase
                     family protein"
                     /protein_id="HDL6318715.1"
                     /translation="MISSVWNERYLADKKREQDHRSPFQRDRARLLHSEAFRCLQAKT
                     QIHAVGEDDFYRTRLTHSLEVAQIGSSLREKLLADVEGFQAVCSEQEFAKNMANLTAL
                     LPDHSLVESLCFAHDLGHPPFGHGGEMALNYKMYAFGGFEGNAQSFRIMTQLEPYTET
                     AGMNLTRRTLLGVMKYPALLSQTSPIEPAEMSEARHINLHHFPQSKGIFDDNNHFFEW
                     VLAPLSEKDRRQFCTVQPNQDPKQPNKTQYKSLDCSIMELADDIAYGVHDLEDAIVGG
                     MVTPQSWQNAEKLLAECQSDWVKQRLPEIREKLFSQHRYERKDVIGALVNHFITNVRW
                     KALPEFDEPLLRYNAYLPESVACVLKILKDFVYQYVICDAKTQRVERKGQRILMELFD
                     ILSSDPMRLLPNNVRERWKNAEESKRARVICDYLAGMSDGQAFKLFESL"
     gene            4960..6279
                     /gene="rlmD"
                     /locus_tag="PXD66_000381"
     CDS             4960..6279
                     /gene="rlmD"
                     /locus_tag="PXD66_000381"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDL6318716.1"
                     /translation="MAIFYSEKSAKKPIKSPACQKATIQSLDYQGLGIAKINGKTWFI
                     ENALPDEQVEFKVLEEKRQYGRGQAVKILKKSANRRMPSCELYGQCGGCQMQHISLEL
                     QRKAKQQALFQRLQKLQTHAIDVQPMIIGNDKHYRRRAKLSIAIQQGKAVIGFRLQNS
                     NQIIPLAHCEVLVEPLANLLPKLKHLIENWQQPKKLGHIELVSADNTIALFFRHLGAL
                     SAQDNHNLRQFATQEQLSLFVMTSENHIEHLCGERPYYQIQGLKLHFSIRDFIQVNAT
                     LNEKMVEKALEWLELSNQDRVLDLFCGMGNFTLPIAERAKSVVGVEGVEPMVQQAREN
                     AVTSGLKNVEFYQTNLDEPFSDKPWAKEPFNKVLLDPARNGALFCLDHLTSLNPERIV
                     YVSCNPATLVRDAEKLLQLGYRLEKAAMIDMFPHTGHLESISLFLKS"
     gene            complement(6419..7600)
                     /locus_tag="PXD66_000382"
     CDS             complement(6419..7600)
                     /locus_tag="PXD66_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HDL6318717.1"
                     /translation="MESRRPSLSFFIILGMMAMLPPLAIDMYLPSFLDIARDLAVSQE
                     KVQTTLAVFTLGFGAGQLFWGPMADSFGRKIIILIGLVGSAIAAYFLTQVQNIETFYL
                     LRLVQGLCAAAPAVVLGALVRDLFERNLFAKLMSVIMIISMLAPLLAPIVGGYIAKYF
                     HWHSIFYVLVAMGILCVVLISWKIPETLAPEKRLPLSFGKTLSNFAILLKHRPTLGYV
                     LIGGLTFAGIFCFLTSGSIVYIGLYGVSQENFGYFFGLNMIVMVTMTAINSKMVVKVG
                     SETMLRIALAMQLLAGIWLALVAIFDLGFWAMAIGIPLYIGMLSTIGSNASAAILEQF
                     PQMAGTANGVAGTARFGVASLVGAGLSHIAVTSAAPMLLAMALCVMLAGVVYYFLCYK
                     R"
     gene            complement(7590..8291)
                     /gene="rsuA"
                     /locus_tag="PXD66_000383"
     CDS             complement(7590..8291)
                     /gene="rsuA"
                     /locus_tag="PXD66_000383"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HDL6318718.1"
                     /translation="MRLDKFIAENTGLTRSQAAKVLKSGVVTVNGNIEKSGAAKISQE
                     DEIYYEDQKLEWVEEGQYFMLYKPQGYICSHDDGEYPTVFQFFDYPLMTKLHTAGRLD
                     VDTTGLVLLTDDGKWSHRITSPKHHCEKTYLVTLADPVEDFYAQRLAEGILLRGEKEP
                     TLPAQLEILDDYNVNLTISEGRYHQVKRMFAALGNKVEALHRWRIGDVVLDERLEEGE
                     FRSLTSEEIASFNGK"
     gene            complement(8536..9507)
                     /gene="menC"
                     /locus_tag="PXD66_000384"
     CDS             complement(8536..9507)
                     /gene="menC"
                     /locus_tag="PXD66_000384"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HDL6318719.1"
                     /translation="MRQVKLYRYQLPIQTGVVLRKQPLKERQGVLVCLQEQEKVGWGE
                     IAPLPSFSQESLEQAENQAVNWLKAWQKGEPETLEQLFPSVAFGLSCALAELNNELGE
                     QANNQSAVLCYGDLEKLHTAFSQHTNPIGKIKIGLNPEQEGEQANLLLHTFPNLRLRL
                     DANRLWSLEQAVKFAEKIANEHKPRIQFIEEPCQALELSRQFATQTDIAIAWDETVRE
                     ANFLVKKEPNLTAIIIKPTLVGSLEKCVNLIQQAQKQGLVAVISSSLESSLGLTQLAR
                     IAQQYTPNSVPGLDTLNLMQHQLLRDWHDSSLPLVGLESEFVREVVV"
     gene            9719..11797
                     /gene="metG"
                     /locus_tag="PXD66_000385"
     CDS             9719..11797
                     /gene="metG"
                     /locus_tag="PXD66_000385"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDL6318720.1"
                     /translation="MANQKRKMLVTCALPYANGAIHLGHMLEHIQADIWVRFQRMRGN
                     EVHFVCADDAHGTPIMLNADKLGITPEQLIEKAKADHEADFASFNISFDNYHSTHSEE
                     NRQITTDIYKTLKANGFIKSKVISQLFDPEKNMFLPDRFVKGTCPKCKAEDQYGDNCE
                     VCASTYSPMDLINPRSAVSGATPVVKESEHFFFDLPSFEGMLKEWTRSGSLQPEIANK
                     MQEWFESGLQQWDISRDAPYFGFEIPDAENKFFYVWLDAPIGYIASFKNLCDRAGIDF
                     DEFWQKDSTTELYHFIGKDIVYFHSLFWPAMLEGSGYRKPTNVFAHGYVTVDGAKMSK
                     SRGTFIQANTYLKHLDPECLRYYYAAKLNDRIEDLDFNLDDFVQRVNSDIVNKLVNLA
                     SRNASFIAKRFEGKLAEKLDDEALFNEFTAQAETIANFYENREFNKAIRAIMELTDKA
                     NKYIDDKAPWVIAKEEGKEAELQAVCSMGIELFRVLMSYLKPVLPKLAERAEAFLQSE
                     LRWDNIATPLFGHTVAPFKSLFSRLEKKQIDAVIDETKALFAEANKKTENQTKGTQKV
                     EQQAVEKQENIAVEPISPEITIDDFAKLDLRAAKVLNCEAVPKSDKMLRFELDLGDHQ
                     RQVFSGIKAAYPNPEALIGRFVIVIANLAPRKMSFGMSEGMILSAGTGGANLFLLDAD
                     SGVQPGMQVK"
     gene            11807..12223
                     /gene="ndk"
                     /locus_tag="PXD66_000386"
     CDS             11807..12223
                     /gene="ndk"
                     /locus_tag="PXD66_000386"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDL6318721.1"
                     /translation="MIQQTLSIIKPDATRRNLIGKILAQLEENGLTIKALKKVHLTRE
                     QAEGFYAEHKGKDFFEPLVEFMTSGSIVVSVLEGENAISHYRALMGATNPDNREKGTI
                     RDLYALSMRENSVHGSDSEESAKREIAYFFVPSEIV"
     gene            complement(12279..12755)
                     /gene="greA"
                     /locus_tag="PXD66_000387"
     CDS             complement(12279..12755)
                     /gene="greA"
                     /locus_tag="PXD66_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDL6318722.1"
                     /translation="MKQIPMTVRGAEQLREELDFLKNVRRPQIIDAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIQEIEGKLGNAQIIDVTKMANNGKVIFGATVQLVNADTDEE
                     VTYRIVGDDEANIKEGLISVNSPIARGLIGKEVDDSVSITTPGGKVEFDIVGVEYI"
     gene            12852..14297
                     /gene="dacB"
                     /locus_tag="PXD66_000388"
     CDS             12852..14297
                     /gene="dacB"
                     /locus_tag="PXD66_000388"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005615286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HDL6318723.1"
                     /translation="MIFKNFTRAFLNKFILSTLLIPSITQAEINTEELLNTLPAGTSV
                     SFIAKNLDTNQIITQYQSDIFMLPASTQKVFTALAAKLTLNDDFRFQTALLTNGKVEN
                     GVLKGNLIARFSGDPELTSGQIYQLMSKLKQQGINKIEGDLILDPSVFASHDKASGWI
                     WNDLTMCFNAPPAAINVDHNCFYVTLNADQPIGEFAKVNVPSAYPVQVFSSAYIVEPK
                     EAPFCQLDVVVHDNNRYQIKGCMARQSQPFGLSFSVQDPTNYGANMLKAQLKSLKIAF
                     NGQVKEPLTAQNGTLLAEHYSEPLPVLLKKMMKKSDNQIADALFRTVANKQHNRPASF
                     QLGSYVIRQLLKTKANIDFKNSVVADGSGLSRHNQVSSRTMLETLEYIAQNEESLKLF
                     ETFPIAGVDGTISGRGSISTEPLAKNLIAKTGSLKGVYNLAGFMKNARGERIAFVQFI
                     NGYSTGELESKTKRAPLNTFENKFYMALFNE"
     gene            complement(14384..14653)
                     /locus_tag="PXD66_000389"
     CDS             complement(14384..14653)
                     /locus_tag="PXD66_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL6318724.1"
                     /translation="MINHNLADFVFEYFTENNLKAGTLRYRYIKENVIDAYSTRVDDA
                     FQGKGIAGELYNALIAFAQEKGLKIKPSCSYIEVKMQRSHRDLIA"
     gene            14825..16414
                     /gene="purH"
                     /locus_tag="PXD66_000390"
     CDS             14825..16414
                     /gene="purH"
                     /locus_tag="PXD66_000390"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005821463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDL6318725.1"
                     /translation="MAIQQALLSVSDKKGIVEFARGLSARGVKLLSTGGTAKLLAEAG
                     LLVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGTDDEVMNQHGIERIDMVVVNLY
                     PFAQTVANPNCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVNNNDFDAILAEMDQHQ
                     NSLTLETRFNLAIKAFEHTAQYDSMIANYFGQKVPPYQGAEEEDLTQPSGQFPRTLNL
                     NFVRKQTMRYGENSHQNAAFYVENELKEASVSTAKQLQGKALSYNNIADTDAALECVK
                     EFSEPACVIVKHANPCGVALGKNILDAYNRAYQTDPTSAFGGIIAFNRELDGETAQTI
                     ADRQFVEVIIAPTVSASAKEVLATKKNVRVLECGEFSEAVKRLDFKRVNGGLLVQDAD
                     QGSVSIDDLKVVSERKPTEAELKDLLFCWKVAKYVKSNAIVYAKDGQTIGIGAGQMSR
                     VYSAKIAGIKAEDEGLQVVGCVMASDAFFPFRDGIDAAAKVGITCVIHPGGSMRDQEV
                     IDAANEHGMAVVLTGMRHFRH"
     gene            complement(16469..17845)
                     /locus_tag="PXD66_000391"
     CDS             complement(16469..17845)
                     /locus_tag="PXD66_000391"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDL6318726.1"
                     /translation="MISVFDMFKVGIGPSSSHTAGPMKAAKEFVDELQNRKFLNKIDR
                     LRVDVYGSLSLTGRGHNTDIAIIMGLMGYLPHNVDIERIEQTIENLKQNKQLIIAESS
                     VEPKVIAFDFYEDMPFHRDFLPRHENGVKFSALSGEELLFQNTYYSIGGGFIVDEANF
                     EQVKNSDLSVPFPYQNAADLLAHCKNNGLPLSSLVWKNELALRSRVEISDYLAMIWNT
                     MEACIERGLNTEGLLPGPLKVVRRASGLRRMLEANSKFNTDPMQIIDWVNMFALAVNE
                     ENAAGGRVVTAPTNGACGIVPAVLAYYQKFIAQLNQDTIERYLLTTSVIGSLYKMNAS
                     ISGAEVGCQGEVGVACSMAAAGLTEIMGGSAEQVCMAAEIAMEHNLGLTCDPVGGQVQ
                     VPCIERNAIASVKAINASRMALRRTSSPQVSLDKVIETMYETGKDMNAKYRETSTGGL
                     AIKILPCD"
     gene            complement(17902..19128)
                     /locus_tag="PXD66_000392"
     CDS             complement(17902..19128)
                     /locus_tag="PXD66_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDL6318727.1"
                     /translation="MAMPSQRFSLTWVLNLFGTAVGAGVLFLPINAGMGGFYPLIIMT
                     LLVGPMTYLAHRGLTRFVLSSKYKGSDITAVVREHFGEQAGKLITLLYFFAIFPILLI
                     YGVGITNTVSSFMENQLHIAPPSRAVLSFMLIAAMIGVMLLSEQVMLKITTCLVYPLV
                     LILLGLSIYLIPQWNTAALQQMPTTGDFLTTLWLTIPVLVFAFNHSPAISSFALSQQK
                     YYQDDKKAEIESAKVLRSTAFILVLFVMFFVFSCVLTLTPEELAQAKVQNISILSYLA
                     NKFDNPIISYFGPLVAFLAIGSSFFGHYLGAREGLEGLVNQMRKEPIDPSKFRKITAI
                     TFLIILWIVATINPSILGFIESLGGPIIAMILFIMPVYAVYKVPALARFKGEFGHLFV
                     LVMGCIAISAIVYGLL"
BASE COUNT         5653 a         4384 c         3797 g         5346 t
ORIGIN      
        1 gaaaagtgat tttttcgaca cagatcgaaa aaatcacttt tcgtacttgc aaaaaattac
       61 gctttattac ttacaatcac tcgcaagcta tctaagcgat aatttgccgt ttcaagttgg
      121 gcaagggaag cgttaagctg ttgttctaag gcttcaactt catcggctcg aatattttta
      181 ttcaccattt tgagtgattc caagcggtgt aattctgctt ttaatttgct atttgcctca
      241 aatttggcag tttcaatcac ctgttggacc tgaggttcta ccgctttttc actcgcttta
      301 attaattttt caatatccgc tcgagccatt ttggcaattt tatttgccat ctgtttattc
      361 accggtttta attggcgttc aagcccggca aaagagacct gcggagcaat attgttgcct
      421 ttgttatcca ctaaaacccg aatcggggtt ggcggtagga aacgggttaa atttagccct
      481 ttcggtgctt gtgcttccac cacataaagc gcctcaagta ataatgtacc ggcaggcaat
      541 ttattattca ccagcaatga gattgctgat ttgccaatat cgccggaggt gattaaatca
      601 ataccattgc ggatcatcgg gtgatcccaa gtgagaaatt ccacctcttc ccgcattaac
      661 gccagttcac gattaaaggt aatggtcgtg ccatcttccg atagccccgg gaaatcaggc
      721 acaagcatat gtccggttgg gctaatcaca atgctctgct cgcctaaatc ttcctgttca
      781 acgccaatca catcaaacaa actcaaggca aaattaacca actgcggatt attatcttct
      841 ttcgcaatat cattagcgag ctgttgagct gcttctccgc cattagaatt cagttctaat
      901 aatctgtcct gtcctttctc cagttcggct ttcaaatact gttggcgttt ataggttttc
      961 gcaacaaaat catcaaattg ggctgaaagt tcaggttttt gcaggaattg ttgcaattct
     1021 tcacctaatt cacggaagat cgctgttccc atcgggcagg tttcttcaaa cgcattcaag
     1081 cccttgtgat accactctgc caacatctgt tgagccgata aattaaagca aggcacataa
     1141 atctgaatgt catttttctg tccgatacga tctaatcgcc caatactttg ctccaataaa
     1201 tccgggttat ccggcaaatt aaacagcact aaatctttag caaattggaa atttcgccct
     1261 tctgaaccga tgcttgaact aatcagcacc tgagcgcctt cttcttgttg agcaaaatag
     1321 gcggcagctt tgtcacgttc aacaatcgac attttctcgt ggaaaacggc agaacgaatc
     1381 gcctcacgct cacgcaaaac ttgctccaaa gcaatcgcag tgtccgcctg tttgcagatc
     1441 accagcactt tttcatcacg atgatttttt aagaaagtga tgagccaatc aatccgaggg
     1501 tcgaattcag cccatttcgc attcgggttc attcgttgga acaaatattc aggatagagc
     1561 aaatcacttt ggctcacact ccccatcatt cccatcactt ttaacgcatt ttggtattgt
     1621 ttcggcattt ctaggctgat ttggtggtat tcccgatgtg ggaagccttt cactccctga
     1681 cgggtatttc taaacaacac ccggctggtt ccatggcggt caattagctc actaatcagc
     1741 tcttgacggg cttgcaagcg gtcattttcg tccgattttt tgctattaat tgcacggaac
     1801 atcggctcgg tatcttgctc gcttaacagc tccgcaatcg cattctgctc gtcattactt
     1861 agcggtttat cgcttaataa gctactcacc gcatctgcca ccggtttata ctgtgcttgc
     1921 tcctgtacaa actcggcata atcataaaaa cgatccgaat ctaacaacgc caagcgggca
     1981 aaatggctct cttgccctaa ttgctccggt gttgccgtta aaagcaagac ggcaggaatt
     2041 tgttgagata aacgctctac caactgatag ccgatagaag attcggtttc cgaccattct
     2101 aaatggtgag cttcatcaac aattaataga tcccagtcgg cagcaagcac ttgattggct
     2161 cgttgcggtg aggtttccag ccagtttagt gaagtaatga ctaacgcttc gctctcaaat
     2221 ggattttcac ttacatcatc gccattttca tcttgtttat caaaatcact acaacgttct
     2281 tcatcgaata acgagaattt cagattaaaa cggcgaagca tttccaccaa ccattggtgt
     2341 tgcaaacttt ccggcactaa taccaatact cgttctgctc tgcccgaaaa tagctgttgt
     2401 tgcaaaatca taccagcttc aatagtttta cccagtccca cttcatcggc taatagcaca
     2461 cgaggagcaa ggcgttgccc gacttctttt gcaatatgta attgatgtgg aatcaggctg
     2521 gctcgaatgc ctcgcaaacc acgcagtggc gactgatatt gcgcttgctg gtgctgcaaa
     2581 gcgttaaagc gtaatgcaaa gcgatctgag cgatcaatct gagccgaaaa aaggcggtct
     2641 tgcggtttgc tgaaactgat tttatgatcg agcaacattt caggcagtac cgcttcttca
     2701 ccgttatcca cccttttgcc caaatagatt acggcacctt gattttccac aacctctgaa
     2761 acctctaact gccagccatc tatactgtta attctatctc cggcttgaaa ctgcactcga
     2821 gttaaaggtg caactgcaat tgcatagacc cgctcttctt ccgctgccgg gaaattgata
     2881 gtgacagtgc gatgatccac cgaagtcacg attcccaaac ctaaattatt ttccgattca
     2941 cttagccaac gctgaccgac taaaaacatt ttttcctcta agtaataagt aaaagcatca
     3001 ttattgtcat ttaaacggct attttaccaa attccgggac cggtcggctt gagtattttt
     3061 gcaaaaaagc acaataaaaa gaccgcttgt gcggttaaat cacacaagcg gtcgttttaa
     3121 ggggtaaatt tagtttttat ctaaatctag atctgcctta tattcaatcc gatttctacc
     3181 ttttacttca atttcaatat ccagctcttg gtcaccacgt ttgaatttta aatcagcatc
     3241 atctctatga atatcagatt ctactaaatg gaaaccttta cgtttagcgt gggcgatcgc
     3301 acttttcgct aagccacgaa catcattacc actgacacga tattctactt caaactcacc
     3361 attaccttga cgatctgctt taactaattc gccacgaggc tgataaattt cacttggaag
     3421 aaaccgcaaa tgctgttgca gaagccaaag ttgctaatac taaaagacct ttagaaacag
     3481 ttttcattga aatctcctat taaataaatg aaacctatta ctgagacctt gcaaaattaa
     3541 caaagttcat tataaacttt caaaaagttt aaacgcctgc ccatcagaca taccggctaa
     3601 atagtcgcaa attacccttg cacgcttaga ttcttccgca tttttccaac gttcccgcac
     3661 attattaggg agtaatcgca tcggatcact gcttaaaata tcgaacaatt ccattagaat
     3721 gcgttgcccc ttgcgttcca cccgctgggt tttggcatca caaatcacat attgatacac
     3781 aaaatctttc aagattttca gcacacaggc aacactttcc ggtaaatagg cattataacg
     3841 tagcaacggc tcgtcaaatt caggcagagc tttccaacgc acattggtaa taaaatgatt
     3901 cactaatgcc ccgatcacat cttttcgctc gtagcggtgc tgagaaaaga gcttttcccg
     3961 aatctccggc aggcgttgtt tcacccaatc ggattggcac tccgccagca atttttccgc
     4021 attttgccac gattgcggtg tgaccattcc acctacaatc gcatcttcca aatcgtgtac
     4081 gccataggca atatcgtccg ctagttccat tatgctgcaa tcgagagatt tatattgcgt
     4141 tttgtttggc tgtttcggat cttgattggg ctgaaccgtg caaaattggc gtcggtcttt
     4201 ttcacttaac ggggctaata cccactcaaa aaagtggttg ttgtcatcaa aaatcccttt
     4261 gctctgtgga aaatggtgca aattaatatg ccgagcctcg ctcatttctg ccggctcaat
     4321 cggcgaggtt tgagacaaga gtgccggata tttcattacc cccagtaagg tgcgacgggt
     4381 cagattcatt ccggctgttt cggtgtaagg ctcaagctgg gtcataatcc gaaaggattg
     4441 agcattaccc tcgaagccgc caaaggcgta cattttataa tttaacgcca tttcaccacc
     4501 gtgcccgaac ggagggtgcc cgagatcgtg ggcaaaacaa agggattcca ccaagctatg
     4561 atccggcaat aaagcggtca gatttgccat attttttgca aattcctgct ccgaacagac
     4621 cgcttgaaag ccttccacat ccgctaataa tttttctcgc aaactgctac cgatttgagc
     4681 cacttccaac gaatgggtta aacgggttcg ataaaaatca tcttccccca cggcgtgaat
     4741 ttgcgttttc gcctgcaaac agcgaaaagc ctccgaatgc aataaccttg cccgatcccg
     4801 ctgaaaaggc gagcggtgat cttgttcccg ttttttatcc gccaaataac gttcattcca
     4861 aaccgatgaa atcatattgc ctccattttc cgccaactgc ttttcttata taatctcccc
     4921 atttcgacac tttatatcat aacaattcat ctgaaaacaa tggctatttt ttattctgaa
     4981 aaatcggcaa aaaaaccgat aaaatcaccc gcttgtcaaa aggcaactat tcaatcgtta
     5041 gattatcaag gcttagggat tgccaaaatc aacggcaaaa cttggtttat cgaaaatgcc
     5101 ctgcctgatg agcaggtgga atttaaagtg ttggaagaga aacgccaata cggacgtgga
     5161 caagcggtta aaattcttaa aaaatctgca aatcggcgaa tgccaagctg cgaactttac
     5221 gggcaatgcg gtggctgcca aatgcagcat atttcgcttg aactgcaacg taaggcaaaa
     5281 caacaggctt tattccaacg cttacagaaa ttgcaaaccc acgctattga tgttcagccg
     5341 atgattatcg gcaacgataa acactaccgc cgtcgggcaa aattaagcat tgcgatccag
     5401 caaggcaagg cggtaatcgg ctttcgtttg caaaattcca accaaattat accgcttgca
     5461 cactgtgaag tgctggttga accgcttgcc aatttgctcc caaaattaaa acacctgatt
     5521 gaaaactggc aacagccgaa aaaattgggg catattgagc tggtcagtgc ggacaatacc
     5581 attgccctat ttttccgcca tttaggggca ttatcggcgc aagataacca caatttgcgt
     5641 caattcgcca cacaagagca attatctctt tttgtgatga ctagcgaaaa tcacattgag
     5701 catctgtgcg gtgaacgccc gtattatcaa attcagggtc taaagttgca tttttcaatt
     5761 cgggatttta ttcaggtgaa tgccacgctc aatgaaaaaa tggtggaaaa agcccttgag
     5821 tggctggaat tatccaacca agatcgagtg ttagatctct tctgtggaat ggggaatttt
     5881 accttgccta ttgctgagcg agcgaaatcg gtggtcggtg tggaaggggt agaaccaatg
     5941 gtgcaacaag cccgagagaa tgcggtgaca agcggtctaa aaaatgtgga attttaccaa
     6001 accaatttag atgagccgtt ttctgacaaa ccttgggcaa aagagccgtt taacaaggta
     6061 ctactcgacc cggcacgcaa cggagcacta ttttgcctag atcacttgac atcactcaat
     6121 cctgagcgga ttgtttatgt ctcttgcaat ccggcaacac tggtgcgaga tgccgaaaaa
     6181 ctgttgcaac taggctacag gctagaaaaa gcggcaatga ttgatatgtt cccccacacc
     6241 gggcatttgg aatctatttc gctgttttta aaatcttagt agatattgcc ccgatggtgc
     6301 gagcgtccac gctcgtacca cacaagcggt taattctgcc taaaaatttg caaaaaatgt
     6361 actattatta accgcttgta gaaatttgtt gggttttcgc aagctaaccc caacctagtt
     6421 atcgcttata acacagaaaa taatacacca caccggctaa cataacgcac aatgccatcg
     6481 ccaaaagcat tggggcagca ctggttacag caatatgtga aagccctgct ccaacaaggg
     6541 aagcaacacc aaaacgagcc gtacctgcta ctccgttagc cgtgcctgcc atttgtggaa
     6601 attgctctaa aatggcggca ctggcattac taccaatggt ggaaagcatt ccgatataaa
     6661 gcggaatacc gattgccatt gcccagaagc ctaaatcaaa aatcgccact aaagccagcc
     6721 aaatgccggc taacagttgc atagctaaag caattcgtaa catcgtttca gagccgactt
     6781 tcactaccat tttgctgtta atcgccgtca tcgtcaccat cacaatcata ttcagcccga
     6841 agaaatagcc gaaattttct tgcgacacgc cataaagccc aatatagaca atcgagcctg
     6901 aggttaaaaa acagaaaata cctgcaaagg ttaagccgcc aatcagcaca taaccaagag
     6961 taggcctatg tttcagtaag atggcaaaat tgcttaaagt tttgccaaaa cttaacggca
     7021 ggcgtttttc gggggctaat gtttcaggga ttttccaact aattaatacc acgcacaaaa
     7081 tccccatcgc cactaacaca tagaaaattg agtgccagtg gaaatatttg gcaatataac
     7141 cgccaacaat cggtgcaagc aatggggcaa gcatagaaat aatcataata accgacatca
     7201 atttggcaaa taaattacgc tcaaacagat ctctcactaa tgctcccagc accaccgccg
     7261 gggctgccgc acataagccc tgtactaacc ttagcaggta aaatgtttca atattctgaa
     7321 cttgggtcag aaaataagcg gcaatcgccg agccgactaa tccgattaaa ataatgattt
     7381 tccgcccaaa gctgtctgcc atcggtcccc agaatagttg ccctgcccca aaacctaggg
     7441 taaataccgc caaggtggtt tgcacttttt cttgcgaaac cgccaagtca cgggcaatgt
     7501 ctaaaaatga gggcaaatac atatcaatgg ctaatggtgg caacattgcc atcataccta
     7561 atatgatgaa aaagctcagg cttggtcttc tactttccat taaaactggc tatctcctct
     7621 gaagtgagcg agcggaattc accttcttcc aacctttcat ctaacaccac atcgccaatt
     7681 cgccagcggt gcaatgcctc gactttattc ccaagggcgg caaacattcg cttaacttga
     7741 tgataacgcc cctcactaat agttaaattc acattgtaat cgtctaaaat ttcaagttga
     7801 gccggcaagg ttggctcttt ctcgccacgc aataaaattc cttctgccaa tcgctgtgca
     7861 taaaaatctt ccaccggatc agcaagcgtc actaagtatg tcttttcaca atggtgcttg
     7921 ggcgaggtaa tacggtgcga ccatttgccg tcatcggtta acagcaccag ccctgtggta
     7981 tccacatcta accgccctgc ggtatgcaat ttagtcatta acggataatc aaagaattga
     8041 aacaccgttg ggtactcacc atcatcgtgt gaacaaatat agccctgtgg cttatagagc
     8101 ataaaatact gcccctcttc cacccactct aatttctgat cttcgtaata aatttcatct
     8161 tcttgactga tttttgccgc accacttttt tcaatattgc cgtttaccgt caccacacca
     8221 cttttcagca ctttagccgc ttgtgaacgg gtcaaaccgg tattttcggc gataaatttg
     8281 tctaaacgca tactattttt ataccataaa ttactttacc ctctcccctc gtgggagagg
     8341 gacagatttt tcttcgtcta gaagaaaaat cagggagagg ggggaaatac gtcttcgtcc
     8401 agaagaaaaa tcagggagag gggaaatgtg caaacttttt tagaaaaatg accgcttccc
     8461 ctctctctgc ttatttcgct gaacgctcat aagcggtctc tctcccacaa ggggagagag
     8521 tgtttaattg ggagattata ccacaacttc cctaacaaat tcgctttcta gcccgaccaa
     8581 cggcaagcta gagtcgtgcc aatctcgcaa taattggtgt tgcattaaat ttaacgtatc
     8641 caaccccggc acactgtttg gggtgtattg ttgggcgata cgagccagtt gggttaagcc
     8701 caaactgctc tcaagacttg aactaatcac cgctactaat ccttgcttct gtgcttgttg
     8761 aattaaattc acgcattttt caagcgaacc aaccagcgta ggtttaatga taatagcggt
     8821 caaatttggc tcttttttta ccaaaaaatt cgcctctctc acggtttcgt cccacgcaat
     8881 cgcaatgtcg gtttgtgtgg caaattggcg ggaaagttcg agcgcttggc aaggttcttc
     8941 gataaattga atacggggtt tatgttcatt tgcaattttt tcagcaaatt tgaccgcttg
     9001 ttccaagctc caaagacggt tggcatcaag ccttaggcgt aaattcggaa aagtatgcaa
     9061 cagcaaattt gcctgctcgc cttcctgttc aggattgaga ccgattttaa ttttaccgat
     9121 tgggttcgta tgttggctaa atgccgtgtg taacttttcc aaatcgccat agcacagcac
     9181 cgccgattgg ttattcgctt gctcgcctaa ttcattattc agttcagcca acgcacagct
     9241 tagcccaaag gcaacagacg ggaaaagctg ctctaacgtt tcaggctctc ctttctgcca
     9301 cgcctttaac caattcacag cttggttttc agcttgttct aggctctctt ggctaaaact
     9361 tggaagcggt gcaatttcgc cccagccgac tttctcctgt tcttgcaaac acaccaacac
     9421 cccttgcctt tctttcaaag gttgcttgcg taacactaca ccggtttgaa tggggagttg
     9481 gtagcggtat aactttactt gtcgcatact atttttatcc aaaataggtt tagaggcgaa
     9541 acatctttcg cccacatcat catttgggaa attgaggcga aaaatgtttc gcctctagcg
     9601 catcatattc taccgcttgt tagcccaaag ctctaataaa ttcacgccaa ctggtaaacc
     9661 tgccactttt gggctaaaat agacggcaat ttttacccta attttaatga gaaaaagaat
     9721 ggctaaccaa aaacgaaaaa tgcttgttac ttgtgccttg ccctatgcta acggtgcaat
     9781 tcatttaggt catatgcttg aacatattca agcagatatt tgggtgcgtt ttcaacgtat
     9841 gcgtggcaat gaagtccact ttgtctgtgc tgatgatgcc cacggcacgc ctattatgct
     9901 taatgccgat aaattaggca ttacccctga gcagttaatc gaaaaagcca aagccgatca
     9961 cgaagcggat tttgccagtt ttaatatcag ctttgataat taccattcca cccacagcga
    10021 agagaaccgc caaatcacta ccgatattta taaaacctta aaagcgaacg gttttattaa
    10081 aagcaaagtg atttcacaac tgttcgaccc agaaaaaaat atgttcttgc cggatcgctt
    10141 tgtgaaaggg acctgcccga aatgtaaagc cgaagatcaa tatggcgata actgtgaagt
    10201 atgtgcctct acctatagcc cgatggattt aatcaaccct cgctcggcag tttccggtgc
    10261 aactccggtg gtaaaagaat cagaacattt cttttttgac ctgccaagct ttgagggaat
    10321 gttgaaagag tggactcgtt ccggctcact ccaacctgaa attgccaata aaatgcaaga
    10381 atggtttgaa agtggtttac agcaatggga catcagccgt gatgcccctt atttcggttt
    10441 tgaaattccg gacgcagaaa acaaattctt ctacgtttgg ttagatgccc ctattggtta
    10501 tatagcatcg tttaaaaatt tatgcgaccg tgcaggcatt gattttgatg agttttggca
    10561 aaaagattcc accaccgagc tttatcactt catcggtaaa gacattgttt acttccacag
    10621 cctgttttgg ccggcaatgt tggaaggcag cggctaccgc aaaccgacca atgtgtttgc
    10681 tcacggttat gtgacagtgg acggggcgaa aatgtcgaaa tctcgtggta cattcattca
    10741 agccaacacc tatttaaaac atctcgatcc ggaatgctta cgctattact atgcggcaaa
    10801 attaaacgat cgcattgaag atttagattt taatttagac gacttcgtgc aacgtgtaaa
    10861 cagcgatatc gtaaacaaat tagtgaatct tgcttctcgc aacgccagtt ttatcgctaa
    10921 acgctttgag ggcaaattag ctgaaaaatt agatgacgaa gcgttattta atgaatttac
    10981 cgctcaagca gaaaccatag cgaatttcta tgaaaaccgt gaatttaata aagctattcg
    11041 tgccattatg gaattaaccg ataaagccaa taaatacatt gacgacaaag ccccttgggt
    11101 catcgccaaa gaagaaggca aagaggctga attacaagcg gtttgttcaa tgggcattga
    11161 gttattccgt gtgctgatga gctacctaaa accggtgtta ccaaaactgg cagaacgtgc
    11221 cgaagcattc ttacaaagcg aattgcgttg ggataatatc gctaccccat tattcgggca
    11281 taccgtggca ccgtttaaat cgctattctc acgccttgag aaaaaacaga ttgatgcagt
    11341 gattgatgaa accaaagcct tatttgccga agcgaataag aaaaccgaaa accaaaccaa
    11401 aggaacacaa aaagtggaac aacaagcggt cgaaaaacaa gaaaatattg cagttgagcc
    11461 aatcagccct gaaattacga ttgatgattt cgcgaaatta gatttacgtg cagccaaagt
    11521 attgaactgc gaggctgtgc caaaatcaga caaaatgtta cgttttgagc tggatttagg
    11581 cgaccaccaa cgccaagtat tctcaggcat taaggcggcc tacccaaatc cggaagcact
    11641 tatcgggcgt tttgtgattg taattgccaa ccttgctcca cgcaaaatgt cgttcggtat
    11701 gtcggaggga atgattttat ctgccggcac aggcggtgcg aacctgttct tattagatgc
    11761 agacagcggt gttcagccgg gaatgcaagt taaataggag aagatgatga ttcaacaaac
    11821 gctttctatt attaagccgg atgctacacg ccgtaattta atcggcaaaa ttttggctca
    11881 attggaagaa aacgggctga ctatcaaagc tctaaaaaaa gtacatctta cccgtgagca
    11941 agcggaagga ttttatgcgg aacacaaagg caaagatttc tttgagccgt tagtggaatt
    12001 tatgacatca ggatctattg tcgtttctgt actggaaggg gaaaatgcaa ttagccatta
    12061 tcgtgcatta atgggggcaa ccaatccgga taaccgtgaa aaaggcacca ttcgcgattt
    12121 atatgcctta agtatgcgtg aaaattcggt acacggttca gattccgaag agtcggcaaa
    12181 acgtgaaatt gcctacttct ttgtaccgag tgagattgtt taataaagta ggggcgaaaa
    12241 ttttttcgcc ccactattgt aattcgccct agcgtagatt aaatatattc tacgccaaca
    12301 atgtcaaact caactttgcc acccggtgtg gtaatgctga ctgaatcgtc cacctctttg
    12361 ccgattaaac cacgagcaat cggggaatta actgaaatta agccctcttt aatattggct
    12421 tcatcatcac ccacaatgcg gtaagtgact tcttcatctg tatcagcatt cactaactgc
    12481 accgttgccc caaaaatcac tttgccgttg tttgccattt tagtgacgtc aataatttga
    12541 gcattgccta atttgccttc aatttcttga atacgcccct cacagaaacc ttgttgttca
    12601 cgggctgcgt gatactccgc gttctctttt aaatcaccgt gttcacgggc ttctgcaatc
    12661 gcatcaataa tttgtgggcg acgcacattt tttaaaaaat ccaattcttc acgcagttgc
    12721 tctgcaccac gcaccgtcat aggaatttgt ttcattaaat ctatttcctt actaaaaatt
    12781 atcggtttac gaattaaaac gaaagggcta ttattcaatt tctcactaaa aatttcaact
    12841 ccccttttat tatgattttt aaaaatttca ctcgtgcttt tttaaataaa tttatcttat
    12901 ctacacttct gattccaagc attacacagg ctgaaattaa taccgaagaa ttattaaata
    12961 ccttgcctgc cggtacttct gttagtttta ttgctaaaaa tttagacact aaccaaatca
    13021 tcacccaata ccaaagtgat atttttatgc tacctgccag tacccaaaaa gtctttacgg
    13081 cactggcagc aaaattaact ttaaacgatg atttccgctt tcaaactgcc cttttaacca
    13141 acggaaaagt agaaaacgga gtgttaaaag gcaatttaat tgcccgtttt agtggcgacc
    13201 ctgagcttac aagcggtcaa atttatcaat taatgagcaa attaaagcaa caaggtatca
    13261 ataaaattga gggggattta attctagacc cgtcggtttt cgccagccac gacaaagcct
    13321 ccggttggat ttggaacgac ttgacgatgt gctttaacgc cccaccggcg gcaattaatg
    13381 tggatcacaa ctgtttttat gtcaccttaa atgccgacca acccattggc gaatttgcta
    13441 aagtgaatgt gccgtccgct tatccggtgc aggtgtttag ctctgcttat attgtcgagc
    13501 cgaaagaagc tccgttttgc cagcttgatg tggtggttca cgataacaac cgctaccaaa
    13561 tcaaaggctg tatggctcgc cagtctcaac cgttcggctt aagtttttcg gtgcaagacc
    13621 caactaatta cggggcaaat atgcttaaag cccagttaaa aagcctgaaa attgccttta
    13681 acgggcaagt aaaagagcca cttactgccc aaaacggcac gctacttgcc gagcattatt
    13741 ccgagccgtt gccggtatta ttgaaaaaaa tgatgaaaaa atcggataac caaattgccg
    13801 atgccctgtt tagaaccgtt gccaacaagc aacacaaccg ccccgcatcg ttccaattag
    13861 gtagttatgt gattcgccag ttattaaaaa ccaaagccaa tattgatttt aaaaacagcg
    13921 tggtggctga tggctcaggg ctttcccgtc ataatcaagt cagcagccga acaatgctgg
    13981 agacccttga atatatcgct caaaacgaag aaagcttaaa actgtttgaa accttcccga
    14041 ttgcaggcgt ggacggtact atttccggca gaggctctat ttcaacagaa ccgcttgcca
    14101 aaaatctgat tgccaaaacc ggctcactga aaggggttta caaccttgcc ggctttatga
    14161 aaaacgcccg tggcgaacgc atcgcttttg tgcaatttat taacggctat tccaccggcg
    14221 agctcgaaag caaaaccaaa cgggctccgc ttaatacatt tgaaaataag ttttatatgg
    14281 cgttgtttaa tgaatagggc ttatattttg tgtgatggat ttttgagaat tgaataagac
    14341 agcggtcgta aatttttgaa attttacgac cgcttgctcc tctctaagca attaaatctc
    14401 gatgacttcg ttgcattttt acctcaatat aactacaact tggcttgatt ttcagccctt
    14461 tttcttgcgc aaacgcaatt aaagcattgt aaagctctcc tgcaatacct ttaccttgaa
    14521 aggcatcatc tactcgggta ctgtaagcat caatcacgtt ctcttttatg tagcgataac
    14581 gtagtgtgcc tgcttttagg ttgttttcgg taaaatactc aaatacgaaa tctgctaaat
    14641 tgtggttaat catagttcct ccgatttttt tcctctattc tacccgcttt aatcacgagt
    14701 gcaagcggtc gattttgcag aaaaatttgc aaaatcgacc acttgtagat gaaaaaagca
    14761 aacgtttgcg ttataatctt tgccgtttat tttcacgtta acactcattt taaggaattt
    14821 aagaatggcg attcaacagg cgttattaag tgtgtctgat aaaaaaggca ttgtcgaatt
    14881 tgctcgtggg ctatcggctc gtggggtaaa attactttct acgggcggaa ccgcaaaatt
    14941 actggctgaa gccggtttgc tagtaacaga agtatcagat tacaccggct ttcctgaaat
    15001 gatggacggg cgtgtaaaaa ccctgcaccc gaaagtgcac ggcggaattc taggtcgccg
    15061 tggtactgat gatgaagtga tgaaccaaca cggcattgaa cgaattgata tggtggtagt
    15121 aaacctctac ccttttgctc aaaccgttgc caatccaaat tgtagccttg aagatgcggt
    15181 ggaaaatatt gatatcggtg gtccgacaat ggtgcgttct gcagcgaaaa atcataaaga
    15241 tgtggcgatt gtggtcaata acaatgattt tgatgctatt cttgccgaaa tggatcaaca
    15301 tcaaaacagt ttaactttag aaacccgttt taatcttgct attaaagcgt ttgagcatac
    15361 cgcacaatac gattcaatga ttgccaacta tttcgggcaa aaagtgccgc cttatcaagg
    15421 ggctgaggaa gaagatttaa cccaaccaag tggacaattc cctcgcacct taaacctcaa
    15481 ttttgtgcgt aaacaaacga tgcgttatgg cgaaaattcg catcaaaacg ctgcattcta
    15541 tgtcgaaaat gagttaaaag aagcctctgt ttccaccgcc aaacagttac aaggtaaagc
    15601 cctttcgtat aataatattg cagataccga tgcggctctt gaatgcgtga aagaatttag
    15661 cgagcctgct tgtgtgattg taaaacacgc taacccttgt ggtgttgctt tgggtaaaaa
    15721 tattttagat gcctacaacc gtgcttacca aaccgatcca acttctgctt tcggtggcat
    15781 tattgccttt aaccgtgaat tagacggcga aactgctcaa accatcgcag accgtcaatt
    15841 tgttgaagtg attattgcac caaccgtttc tgcttctgcc aaagaagtgt tagccactaa
    15901 gaaaaacgtg cgtgtgcttg aatgtggcga gttctcagaa gctgtaaaac gcttagattt
    15961 caaacgggta aacggcggat tattagtgca agatgccgac caaggctctg tatctattga
    16021 cgatttaaag gtggtttcag agcgtaaacc aactgaagct gagctaaaag acctattgtt
    16081 ctgctggaaa gtggcgaaat atgtgaaatc gaatgcgatt gtgtatgcca aagacgggca
    16141 aactatcggt atcggtgcag ggcagatgag ccgtgtttat tccgcaaaaa ttgcaggtat
    16201 taaagcggaa gacgagggct tacaagttgt gggttgtgta atggcttccg atgcgttctt
    16261 cccgttccgt gatggtattg acgctgctgc aaaagtaggg attacttgtg tgattcaccc
    16321 aggtggttca atgcgtgatc aagaagtgat tgatgccgcc aacgaacacg gtatggcagt
    16381 ggtactaacc ggaatgaggc atttccgtca ttaatgttct acaaactaaa aggctacctt
    16441 attttgataa ggtagccttt ttcattaatt aatcacaagg taaaattttg attgctaagc
    16501 caccggttga ggtttcacgg tattttgcat tcatatcttt gccggtttcg tacattgttt
    16561 caatgacttt atctaaactt acctgaggcg aagaagtacg gcgtagtgcc attctgctgg
    16621 cattaatcgc tttgactgaa gcgattgcat ttctttcaat acaaggcact tgcacttgtc
    16681 cgccaacagg gtcacaagtt aggcctaaat tgtgctccat tgcaatttct gcagccatac
    16741 acacctgttc agcactgcca cccataattt ccgttaaacc tgctgccgcc attgaacaag
    16801 caacaccaac ttccccttgg caacctactt cagcaccgga aatagaggca ttcattttgt
    16861 ataacgaacc aatcacactg gtagtcaata aataacgctc aatagtatct tgattaagct
    16921 gtgcaataaa tttttggtag tacgccaaca cagccggaac aataccacag gctccattgg
    16981 taggtgcggt gactactcgc ccacctgccg cattttcttc attcacggca agggcaaaca
    17041 tattcaccca atcgataatt tgcattgggt cggtattgaa tttactgttt gcctctaaca
    17101 ttctgcgtag cccactggca cggcgtacca ctttcagagg cccgggtagt aacccttccg
    17161 tattcaaacc acgctcaata caggcttcca ttgtattcca aatcattgcc aaataatccg
    17221 aaatttctac tctactgcgt aaagcaagct catttttcca gactaaagaa gaaagcggta
    17281 acccgttatt cttacaatgt gcaagtaaat ctgctgcatt ctgatatggg aatggaacag
    17341 aaagatctga atttttaact tgctcaaagt tagcttcatc aacaataaag ccaccaccaa
    17401 tagaataata ggtgttttga aatagcaact cttcaccgct caaggcacta aatttcacac
    17461 cattttcgtg ccgaggtaag aaatctcgat gaaatggcat atcctcataa aaatcgaacg
    17521 caatcacttt aggctcaaca ctgctttcag caatgataag ttgcttattt tgttttagat
    17581 tttcaatcgt ctgctcaata cgttcaatat ccacattatg aggcaaatac cccattaatc
    17641 ccattatgat ggcaatatcc gtattatgtc ctcgcccggt gagtgataat gaaccataga
    17701 catctacccg caagcggtca attttgttta aaaatttgcg attttgtaat tcatcgacaa
    17761 attcctttgc cgctttcatc ggccccgcag tatgggaact ggaaggccca atccctactt
    17821 taaacatatc aaatacacta atcataaaca caaattcaca tagaaaataa aagggcgtat
    17881 tctacattga ttttagaaag attacagtaa cccatatact attgctgaaa tagcaataca
    17941 acccataaca agcacaaata aatggccaaa ttcaccttta aaacgtgcaa gtgccggtac
    18001 tttatatacg gcatagacag gcataataaa caagatcatc gcaataatcg gcccaccaag
    18061 gctttcaatg aagcctaaaa tgcttggatt gatagtcgca acaatccata aaatgatcaa
    18121 aaaagtaata gcggtaattt tgcggaattt gcttggatca atcggctctt tacgcatttg
    18181 attaaccaat ccttctaacc cctcgcgcgc acctaaataa tgcccaaaga aagagctacc
    18241 aatcgccaaa aaagcaacca acggtccaaa ataggaaata atcggattat caaatttatt
    18301 cgcaaggtaa gaaagaatcg aaatattttg cactttagct tgtgctaatt cttcaggtgt
    18361 caaagttaat acacagctaa atacaaagaa catcacaaat aacaccaaga taaaggcggt
    18421 agaacgtaat actttagctg attcaatttc agccttcttg tcatcttggt aatatttctg
    18481 ttgagaaagt gcaaaagaag aaatagccgg tgaatggtta aaagcaaaga ctaaaacagg
    18541 aatagtcagc caaagtgtag taaggaagtc tcctgtagtt ggcatttgct gtaaggcagc
    18601 tgtattccac tgtggaatta aataaataga taagcccaat aaaatcaaca ctaatggata
    18661 cacaagacaa gttgtgattt ttaacatgac ttgctcactt aataacatta ctccgatcat
    18721 agcagctatt agcataaacg ataatacagc cctagatggt ggcgcgatat gaagctgatt
    18781 ctccataaaa gaactcacgg tattggtaat acctacacca taaatcagta aaatcggaaa
    18841 aatggcaaag aagtacagta gcgtgatcaa tttaccggcc tgttcaccaa aatgttcacg
    18901 aacaacagcc gtaatatcgc tccctttgta ttttgaagaa agcacaaaac gtgttaagcc
    18961 acggtgagct aaatatgtca ttggtccaac taacaaagtc ataataataa gtgggtagaa
    19021 cccacccata cctgcattaa ttggcaaaaa taaaacgccg gctcctaccg cagtaccaaa
    19081 taaatttagt acccaagtaa gagaaaaacg ttgagatggc atagccatag taatctcctt
    19141 taaagaatta tctaacaagc tatgctatat tagcgatggg
//
LOCUS       DANLXF010000019        19088 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.019, whole genome shotgun sequence.
ACCESSION   DANLXF010000019 DANLXF010000000
VERSION     DANLXF010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 19088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19088
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.019"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            13..963
                     /gene="tal"
                     /locus_tag="PXD66_000393"
     CDS             13..963
                     /gene="tal"
                     /locus_tag="PXD66_000393"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDL6318728.1"
                     /translation="MSQLDTLSKMTVIVADTGDIEAIKLYKPKDATTNPSLILSASAL
                     PQYAPLIEEAINYAKAQSSDKDQQLVDAEDKLAVNIGLEILKLVPGRISTEVDARFSY
                     DTEKTIAKARKLIGLYNDAGISNDRILIKIASTWQGIKAAEQLEKEGINCNLTLLFSQ
                     AQARACAEAGVYLISPFVGRILDWYKAHEGKEYAPAEDPGVISVTNIYNYYKQHGYDT
                     IVMGASFRNVGEIIELAGCDRLTISPPLLKELQESDEKLVCKLDYKGEIKPRPEKMTE
                     AEFYWEHNQEPMAVEKLAEGIRKFAIDQEKLEAMLAEKLQ"
     gene            complement(1025..1298)
                     /locus_tag="PXD66_000394"
                     /pseudo
     CDS             complement(1025..1298)
                     /locus_tag="PXD66_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599987.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
     gene            complement(1329..1793)
                     /locus_tag="PXD66_000395"
     CDS             complement(1329..1793)
                     /locus_tag="PXD66_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDL6318729.1"
                     /translation="MKKLALAVALMSSFALVGCANTDVFSGSVYSGEQAKEARSIAYG
                     TIVSVRDVKIQARSEGVIGTVGGGVLGGVAGNAIGGGTGQAIATAVGAVAGAVIGNQV
                     EQKASQVSSLEMVIRKDDGKEIVVVQKKEDGFVPGKRVRIVGSNSDLNVSLL"
     gene            complement(1814..2413)
                     /locus_tag="PXD66_000396"
     CDS             complement(1814..2413)
                     /locus_tag="PXD66_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL6318730.1"
                     /translation="MDKIIAENAKFFEKMTACFGDLSQFEQKIRQIAEIAGIDLAAYE
                     IDHLAVRMNQTETAEQWKILLLDGATLLKESEVNGRPIGLFALIQAVEFCGQFVKIVE
                     LPFPKNKVYLEEGWEHIELVFPMLDNESSEQWVSRALSHFRLADNSEIKLKISQPQVE
                     GERLPNPSIAISLRNVTFCNPYCLKLHPYDIKSVIMSEN"
     gene            2447..4174
                     /gene="argS"
                     /locus_tag="PXD66_000397"
     CDS             2447..4174
                     /gene="argS"
                     /locus_tag="PXD66_000397"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDL6318731.1"
                     /translation="MNIQQILSDKIKQAMVASGADSNVEPLVRQSGKPEFGDYQANGA
                     MGAAKKLGMNPREFAQKILENADLNGIADKLEIAGPGFINIFLNPEWLAENTNSSLQA
                     VNFGIKTANPQTIVIDYSSPNVAKEMHVGHLRSTIIGDAVVRTLAFLGNNVIRANHVG
                     DWGTQFGMLIAYLEKMENEHASEMELSDLEAFYRAAKEHYDSDEAFAEKARNYVVKLQ
                     SGDKYCLTMWKKLVDITMHHNQENYNRLNVTLTEKDVMGESLYNPMLPEIVADLKAKG
                     LAVEDDGALVVFLDEFKNKDGDPMGVIVQKKDGGFLYTTTDIAAAKYRYETLKADRAL
                     VFSDSRQAQHMQQAWLITRKAGYVPDSFSLEHPFFGMMLGKDGKPFKTRSGGTVKLKD
                     LLDEAVERADKLIAERSTELTAEEKAAVVEAVAIGSVKYSDLSKNRTTDYVFDWDNML
                     TFEGNTAPYMQYAYTRIRSIFARANIDENALSGSIQLNEPKERALAVKLLQFEEALNG
                     VAKEGMPHILCQYLYELAGSFSSFYEACPILNAEETTKNSRLKLAALTAKTLKQGLDL
                     LGIKTVEKM"
     gene            4364..5530
                     /gene="cgtA"
                     /locus_tag="PXD66_000398"
     CDS             4364..5530
                     /gene="cgtA"
                     /locus_tag="PXD66_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HDL6318732.1"
                     /translation="MKFIDEALIRVEAGDGGNGCVSFRREKFIPKGGPDGGDGGDGGD
                     VYLIADENLNTLIDYRFEKRYAAGRGENGRSAGCTGHRGQDITLRVPVGTRAIDNDTQ
                     EIIGDLTRHGMKMLVAKGGYHGLGNTRFKSSVNRAPRQKTNGTPGEKRDLKLELLLLA
                     DVGMLGLPNAGKSTFIRSVSAAKPKVADYPFTTLVPSLGVARVGADRSFVVADIPGLI
                     EGAADGAGLGIRFLKHLERCRILIHLVDIMPIDESDPAHNISVIESELYQYSEKLAEK
                     PIWLVFNKIDTIGEEEAAERAKDIAEQIGWEGDYYLISAATGQNVQALTRDIMDFIEA
                     NPREVEEENKAPEEVKFKWEDYHNEAMQNAVEEDWDDDWSEEDDEGIEFIYERH"
     gene            5615..6232
                     /locus_tag="PXD66_000399"
     CDS             5615..6232
                     /locus_tag="PXD66_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HDL6318733.1"
                     /translation="MLSSTSCDLFNIPFFQFAQMKKYCPEEIPRIKAEYKTQWEIWKK
                     LHQEVSRQLGQPFAPPHIEKWCNGWQVRAHFFAYYKYEFNRNSAAILSVILNRRRLQV
                     SLDWHCYRADRSQINVHQYNQWWENLDREKFADFEIWHGSESEYDDFRTVSQMSEQAF
                     TLTNEEDFWCIGKNVEKDDLDNIDVVEFIYQTIRALLPLYEKCHQ"
     gene            complement(6279..8237)
                     /locus_tag="PXD66_000400"
     CDS             complement(6279..8237)
                     /locus_tag="PXD66_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HDL6318734.1"
                     /translation="MIAELGNYTLALALAISILLAVLPLWGAEKQNSTLIALARPMTW
                     AMFLALTVSFGSLFYLFAVNDFTVQYVVNNSNSRLPLQYRLSAVWGAHEGSLLLWVWL
                     LSLWSVGVATFTRKMPEDAVARVLGLMGVIGIGFLMFVIFTSNPFDRTFPNFPIDGKE
                     LNPMLQDVGLIFHPPLLYMGYVGFSVAFAFAIASLMTGRLDTAWARWSRPWTMAAWVF
                     LTLGIVLGSWWAYYELGWGGWWFWDPVENASLMPWLAGTALIHSLAVTEKRGTFKAWT
                     VLLAILAFSLCLVGTFLVRSGILVSVHAFASDPTRGLYILAYLVLVVGGSLLLYAFKG
                     SEIKSLDNYERYSRETMLLINNILLMAFLSVVFLGTLLPLIHKQIGLGTISIGAPFFD
                     QMFLILMVPFSFVLGIGPLVKWRRDQVSAIRKPVIIATILMALLGFGLPYLFRNTITV
                     SVVLGTMMSVFIVLLSLYELHQRATHRHSFLSGIFKLSRSHWGMVLAHLGVAMTVFGI
                     AFSQNFSIERDVRMNVGDKAEILDYQFEFKGIKITDGANYQGGTAELEITRNGKYEAT
                     LNAEKRFYTVSRMGMTEAAIDWGFTRDLYAALGEKLEDNSWAVRLYYKPFIRWIWIGG
                     LFMALGGLLCMLDKRYRLRIENKKEVIA"
     gene            complement(8234..8791)
                     /gene="ccmE"
                     /locus_tag="PXD66_000401"
     CDS             complement(8234..8791)
                     /gene="ccmE"
                     /locus_tag="PXD66_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HDL6318735.1"
                     /translation="MNPRRKSRLKVVVSILLGLSVAAGLTLYALSQNIDLFYTPSEIV
                     NGKNDDPKQKPEVGQRIRVGGMVVEDTVKRDDKSLKVVFDLNDIGPSITVEYEGILPD
                     LFREGQGIVAQGVLIEPTRLKATEVLAKHDENYMPPELGDKLKEQHKKSGVSEQDLKG
                     ESETDRKMKAEAEAEKANNVQGESK"
     gene            complement(8977..9174)
                     /gene="ccmD"
                     /locus_tag="PXD66_000402"
     CDS             complement(8977..9174)
                     /gene="ccmD"
                     /locus_tag="PXD66_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HDL6318736.1"
                     /translation="MTFQFESISDFFAMGGYGFYVWLSYGVSFIAMIGLIWLSRREQK
                     AILAQAKKEIVREEKLKQTSQ"
     gene            complement(9189..9926)
                     /locus_tag="PXD66_000403"
     CDS             complement(9189..9926)
                     /locus_tag="PXD66_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HDL6318737.1"
                     /translation="MWKWLHPYAKSETQYHLLGKIQPFLGWLSLIMLAAAFVWGLAFA
                     PKDYQQGDSYRIIFIHVPAAIWSMGVYGSMAVAGLVALVWQIRQANLAMISMAPIGLV
                     FAFISLATGAIWGKPMWGTWWVWDARLTSALVLFFLYIGVMALYSAFQDRQTGAKAAA
                     VLSVVGVINLPIIHFSVEWWNTLHQGATITKFDKPSMAVEMLIPLLLAIFGSLIFTAW
                     FSIWRYRIALLKDERNRPWVKQLAKSV"
     gene            complement(10126..10788)
                     /gene="ccmB"
                     /locus_tag="PXD66_000404"
     CDS             complement(10126..10788)
                     /gene="ccmB"
                     /locus_tag="PXD66_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005565617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HDL6318738.1"
                     /translation="MILLTIIQRELTIAFRKPSEILNPLWFFLMVITVFPILMGPNPE
                     LLGKIAGGIAWVAALLSALLSFERLFRDDYLDGSLEQLMLLPLALPQVALAKVIAHWL
                     LTGLPLILLSPIAAILLSLETHVWWALVLTLLLGTPILSCLGAIGVALTVGLRKGGTL
                     LSLLILPLFLPVLIFAAAVLEAATLNMGYSGQLAIIGAILAVTLTFSPFAIAGALRIS
                     LN"
     gene            complement(10785..11426)
                     /gene="ccmA"
                     /locus_tag="PXD66_000405"
     CDS             complement(10785..11426)
                     /gene="ccmA"
                     /locus_tag="PXD66_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HDL6318739.1"
                     /translation="MNQTAQYQLKLNQIACERGETRLFEGCSFTVQSGEWVQIEGHNG
                     IGKTSLLRILAGLFLPAEGEVLWNDIAIQKQRDHYYSELFYLGHYSGVKPELTPWENL
                     RFYQKIQQLPQDDDALWHALTKVSLIGREDLPCSHLSAGQQRRVALAKLWLTNAKLWI
                     LDEPFTAIDKKGVADLIAHIEQHCEQGRLVIFTSHQAAESDKVRIISLDQFKV"
     gene            complement(11570..12280)
                     /gene="hda"
                     /locus_tag="PXD66_000406"
     CDS             complement(11570..12280)
                     /gene="hda"
                     /locus_tag="PXD66_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HDL6318740.1"
                     /translation="MQLPLPIHQIDEDTFDNFYSQNSEVLLGSLKRNFTDVQQPFFYI
                     WGGKSCGKSHLLKAVSNHFLLNNQTSSYIPLTKANYFSPLVLDNAELLNVICLDDIQA
                     IAGDAEWELAIFNLFNQIREQQGLFNEGQKTLLLISADCPPHQLNIQLPDLRSRLTWG
                     EVYQLNDLTDEQKCQILQLNAHQKGVELSDEVAHFLLKKAGTDLEQLSKILDKLDKAS
                     LQAQRKLTIPFVKEILSL"
     gene            12550..14151
                     /locus_tag="PXD66_000407"
     CDS             12550..14151
                     /locus_tag="PXD66_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL6318741.1"
                     /translation="MKNMTKLSLITLALAASSSVFAAPKTFVYCLEASPSALSPVLST
                     DGASFDVNALPIYNKLTDFEDGTTNVIPSLAEKWDISEDGKTYTFHLRKGVKFHDNKE
                     FKPSRELNADDVVFSINRQFDPNHPFHKVSGGNYEYFIGMDMQNIIDKVEKVDDHTVK
                     ISLKVPNAPFLANLAMDFNAVYSAEYAEQSLKAGKPERIDTNPIGSGPFQFVDYQKDA
                     AVRYKAFENYWQGTAKIDRLVFAITPDASVRLAKLQKGECHAMPYPNPADIEDLKKDP
                     NIELMSQSGMNIGYLALNSSIKPLDNQKVRQALNHAVNKQAIVDAVYQGAGQVAKNPI
                     PPTMWSYNEAVQDYDYNPEKAKALLKEAGFENGFDTELWAMPVSRPYNPNARRMAELI
                     QEDWKKVGVNAKIVSYEWGEYLKRLRQGEAATSMIGWTGDNGDPDNFLNTLLSCSAVE
                     AGSNYAKFCHKPFEEVVLGAAQESDKAKRTELYKQAQVIFKEQAPWITIAHSTVYFPV
                     RKEVKGYVMSPFSLHNFYKVDLADK"
     gene            14423..15427
                     /locus_tag="PXD66_000408"
     CDS             14423..15427
                     /locus_tag="PXD66_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005712947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL6318742.1"
                     /translation="MFSFILKRVLMVIPTFIAITLITFALVHFIPGDPIEIRMGERGL
                     SPEVHQQMMEQLGLNRPLFEQYISYIKGVLHGDLGNSFRNNEPVLKEFFTLFPATVEL
                     AFFALLWSLIFGIILGVIAAVKKESWISHTVTTLSLTGYSMPIFWWGLILILYFSTPL
                     GLPASGRLPSEYWIEAETGFMLIDTWNSDEPGAFLAAIKSLILPSIVLGTIPLAVVTR
                     MTRSSMLEVLGEDYIRTAKAKGLNTTRIVIVHALRNALIPVVTVVGLIVGQLLSGAVL
                     TENIFSWPGIGKWIIDAINSRDYPVLQGSVLIIATIIILVNLTVDLLYGIVNPRIRHS
                     "
     gene            15529..16422
                     /locus_tag="PXD66_000409"
     CDS             15529..16422
                     /locus_tag="PXD66_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL6318743.1"
                     /translation="MSGITAVNPPAPKTPLQEFWYYFCQNRGALIGLTFIAIVFVACV
                     FAPWVAPFDPIEQNRSALLLPPAWLEGGNSAHLLGTDDIGRDILSRIIYGSRLSVFIG
                     LVIVLLSCVFGVILGLLAGYYGGTLDIIIMRFVDIMLAIPSLLLTIGVVTILGPSLIN
                     AAIAIAIVSIPSYVRLTRASVLSEKNRDYVTASRVAGASVLRLMFVVILPNCLAPLIV
                     QMTMGISNAILELAALGFLGIGAQPPTPELGTMLAESRGFMQSANWLVTIPGLAILSL
                     VLAFNLMGDGLRDALDPKLKQ"
     gene            16433..17422
                     /gene="dppD"
                     /locus_tag="PXD66_000410"
     CDS             16433..17422
                     /gene="dppD"
                     /locus_tag="PXD66_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDL6318744.1"
                     /translation="MALLTVDQLSVHFGETKTPFKAVDRISYEVNEGEVLGIVGESGS
                     GKSVSSLAIMGLIDFPGRVMAEKLYFNGNDLLALKPKEKQQIVGADVSMIFQDAMTSL
                     NPSYTVGYQIMEALKVHQGGSKASRRERAIELLTMVGIPDPESRLEVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIDLLLELQRKENMALILITHDLALVA
                     ESAHRIIVMYAGQVVEEGKAEQIFKAPLHPYTQALLKALPEFAEGKSRLQSLPGVVPG
                     KYDRPQGCLLNPRCPYATEKCRTIEPAMQIINGRQVKCHTPLNAQGEPIRIAV"
     gene            17550..18539
                     /locus_tag="PXD66_000411"
     CDS             17550..18539
                     /locus_tag="PXD66_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL6318745.1"
                     /translation="MKENNSLPPFLKAVDLKKYYPVKKGMFAKPKTVKALDGVSFTLE
                     RGKTLAIVGESGCGKSTLGRMLTMIESPTEGELFYNGQNFLVNDKETAQLRRKKIQIV
                     FQNPYSSLNPRKKVGSILEEPLLINTNLSAKERKEKVLEMMAKVGLKPEFYDRYPHMF
                     SGGQRQRIAIARGLMLQPDIVVADEPVSALDVSVRAQVLNLMMDLQEEMGLSYVFISH
                     DLSVVEHIADEVMVMYLGRCVEQGDTKTIFSNPRHPYTQALLSATPRLNPEQRRERIK
                     LTGELPSPLNPPKGCAFNARCRFATDRCYVERPELKTYQDGTRIACFVVEENN"
     gene            complement(18773..>19088)
                     /locus_tag="PXD66_000412"
     CDS             complement(18773..>19088)
                     /locus_tag="PXD66_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248476.1"
                     /note="Bacteria have multiple sigma factors which are
                     active under specific conditions; the sigma factor binds
                     with the catalytic core of RNA polymerase to produce the
                     holoenzyme and directs bacterial core RNA polymerase to
                     specific promoter elements to initiate transcription;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HDL6318746.1"
                     /translation="DLADDDEENHFFDNYGHWKEEYYPSSWQEEAVYSKEFWILFEVC
                     LSNLPAKQARVFMMREYLEATSDEICRVVNITSDNLYTLIYRARLQLQQCLSRKLTER
                     NR"
BASE COUNT         5751 a         4193 c         3974 g         5170 t
ORIGIN      
        1 aggaatcaga ctatgagcca acttgacacc ttaagcaaaa tgaccgttat cgttgctgac
       61 actggtgata ttgaagcaat caaactttat aagccaaaag atgcaacaac aaacccgtct
      121 ttaattttaa gtgcatcggc attaccgcaa tatgctccat taattgaaga agcgattaat
      181 tacgcaaaag cacaaagtag cgataaagat caacagttag ttgatgctga agataaatta
      241 gcggtaaata ttggtttaga aatcttaaaa cttgtacctg gacgtatttc tacagaagtt
      301 gatgctcgtt tttcttatga tactgaaaaa actatcgcta aagcacgtaa attaattgga
      361 ttatataacg atgcaggcat cagtaatgat cgcattttaa ttaaaattgc ctctacttgg
      421 caaggtataa aggcagcaga acagttagaa aaagaaggca ttaactgtaa cttaacgtta
      481 ttattctctc aagctcaagc tcgtgcgtgt gcagaagcag gggtttactt aatctctcca
      541 ttcgttggtc gtattttaga ctggtacaaa gctcacgaag gtaaagaata tgctccagct
      601 gaagatccag gtgtaatttc agtaaccaat atatacaatt actacaaaca acacggttat
      661 gacactattg taatgggtgc aagtttccgt aatgtaggag agatcattga gttagctggt
      721 tgcgaccgtt taaccatctc accaccgtta ttaaaagagc tacaagagag cgatgaaaaa
      781 ttagtttgca agctagatta caaaggtgaa atcaagcctc gtccagaaaa aatgacagaa
      841 gcagaattct actgggagca taaccaagag ccaatggcag tagaaaaatt agctgaaggc
      901 attcgtaaat ttgcgattga ccaagagaag ttagaagcaa tgcttgctga aaaacttcaa
      961 tagacaacgc ataaaaaaac cggcattaac gataattaat gccggtttta atttctataa
     1021 atgattattt aaactcataa cgtctaatcg tttttagatc gctatcttca tttttaattt
     1081 catcggcaag atgaacacga gcaccaaccg ctgcatgtgc atcatactca cgagttagag
     1141 tcacagtttc accattatct cgttgaactg taagttcaac aacatcgttt gatactgctt
     1201 tgctagataa atgttgcatt acctttatca gaataaacat ctgcagtaca agctgctaac
     1261 ataagtgaag ctaatacagc taaagaaagt tttttcatta ataatatatc ctctaggaaa
     1321 aactaaaatt aaagcagaga aacattcagg tctgaattag accctacgat tctcacacgt
     1381 ttgccaggca caaaaccgtc ttctttcttt tgaacgacca cgatttcttt accatcatct
     1441 ttgcgaataa ccatttccaa tgaagaaact tgactcgctt tttgttctac ttgattacca
     1501 attaccgcac cggcaactgc ccccactgcg gttgcaatcg cttgccctgt accaccgcca
     1561 atcgcattac cggcaacgcc acctaataca ccaccgccga cagtaccgat cacaccttca
     1621 ctgcgtgctt gaattttcac atcacgcact gaaacaatag tgccataagc gatagaacga
     1681 gcttcttttg cctgctcacc ggaataaacg cttccgctaa acacgtctgt atttgcacag
     1741 ccaactaaag caaaacttga cattaatgcg acagctaaag ctaatttttt cattttgttt
     1801 gttctccgaa acgttaattt tctgacataa ttacactttt tatatcataa gggtggagct
     1861 ttagacaata aggattacaa aaagttacat ttctcaagct gatcgcaatg cttggatttg
     1921 gcaatctctc cccttctacc tgaggttggc tgatttttaa tttaatctca gaattatcag
     1981 caagtctaaa gtgcgacaat gcccttgaca cccattgttc cgaactttcg ttgtctaaca
     2041 tcggaaaaac cagctcaata tgttcccagc cttcttctaa ataaacctta tttttcggaa
     2101 aaggcaactc gacaatcttt acaaattgtc cgcaaaattc aaccgcttgt attaatgcaa
     2161 acagccctat cggtctgccg tttacctcgc tctctttaag taaggttgcc ccatcaagca
     2221 aaaggatctt ccactgttct gcggtttcgg tttggttcat tcttaccgca agatgatcga
     2281 tttcatacgc tgctaaatca atgccggcga tttcagcaat ttgtcggatt ttctgttcaa
     2341 attgcgataa atcgccaaaa caagcggtca ttttctcgaa aaattttgca ttttctgcta
     2401 taattttgtc catttttttg taaatagaat ttaaataggt taaacagtga atattcaaca
     2461 aattttatca gataaaatta aacaggcaat ggtagcaagc ggtgcagaca gcaatgtcga
     2521 gcctttagtc cgccaatcgg gcaagccgga atttggcgat taccaagcca atggtgcgat
     2581 gggtgcagcg aaaaaattgg ggatgaatcc gcgtgaattt gcccaaaaaa tcttagagaa
     2641 tgccgatttg aacggtattg ccgataaatt agaaattgcc ggtccgggct ttatcaacat
     2701 tttcttaaac ccagaatggc tggcggagaa cactaacagt tcattgcaag cggtcaattt
     2761 tggcataaaa actgcaaatc cgcagacgat tgtgatcgac tactcctcgc caaacgtagc
     2821 aaaagaaatg cacgtcgggc atttgcgttc caccattatt ggcgatgcgg tagttcgtac
     2881 ccttgcgttt ttaggcaata atgtgattcg tgccaaccac gttggcgact ggggtactca
     2941 attcggtatg ctgatcgctt accttgaaaa aatggaaaat gagcacgcca gcgaaatgga
     3001 gctaagcgat ttagaagcct tctatcgtgc ggcaaaagag cattatgatt cagacgaagc
     3061 attcgccgaa aaagcccgta actatgtggt gaaattacaa agcggcgata aatattgctt
     3121 aacaatgtgg aaaaaattgg tcgatattac aatgcaccac aatcaagaga actacaatcg
     3181 cttaaatgtt acgctcaccg aaaaagatgt aatgggcgaa agcctctaca atccaatgtt
     3241 gcctgaaatt gtggcggatt taaaagccaa aggcttagcg gtggaagatg acggtgcttt
     3301 agtggttttc ctcgatgaat ttaaaaataa agatggcgac ccaatgggcg taatcgtgca
     3361 gaaaaaagat ggcggtttct tatataccac taccgatatt gcggcagcaa aatatcgcta
     3421 cgagacctta aaagctgacc gagccttagt cttttctgac agccgccaag ctcaacatat
     3481 gcaacaggca tggctcatta cccgcaaagc gggctatgtg ccggacagtt tcagccttga
     3541 acacccgttc ttcggaatga tgctaggcaa agacggcaaa ccgttcaaaa cccgtagcgg
     3601 tggcacagtc aaactcaaag atttattaga tgaagcggta gagcgtgccg ataaactgat
     3661 tgccgaacgt tcaaccgagc taactgcgga agaaaaagcc gccgtggttg aagcggttgc
     3721 gattggttcg gtgaaatatt ctgacttatc gaaaaaccgc accaccgatt atgtgttcga
     3781 ttgggacaat atgctgacct ttgaaggcaa taccgccccg tatatgcagt atgcctacac
     3841 ccgcattcgc tccatctttg cccgtgcgaa cattgacgaa aatgcattga gcggttcaat
     3901 ccaactcaat gagccaaaag agcgtgcatt agcagtgaaa ctattgcaat ttgaagaggc
     3961 attaaacggc gtggcaaaag aaggtatgcc acatatttta tgccaatatt tatacgagct
     4021 tgcaggtagt ttctccagtt tctacgaggc ttgcccgatt ctcaatgccg aagaaacaac
     4081 caaaaacagc cgtttgaaac tcgctgcctt aacggcgaaa accttgaaac aagggttgga
     4141 tttactcggt attaaaactg tagagaagat gtaatacaaa ctctctcccc ttgtgggaga
     4201 gagaccgctt atgagcgttc agcgaaataa gcagagagag gggacaagcg gtaattttta
     4261 ttaaaatttt tacacatttt cccctctccc tgatttttct tctgaacgaa gaaaaatctg
     4321 tccctctccc acaaggggag agggcatata aaaggaaaca aatatgaaat ttattgacga
     4381 agccctgatt cgtgtagaag caggcgatgg cggtaatggc tgtgtgagct tccgccgtga
     4441 aaaatttatc ccgaaaggcg gacctgacgg tggtgatggt ggcgatggtg gtgatgtgta
     4501 tttaattgcc gatgaaaacc taaacaccct tatcgactac cgctttgaaa aacgttatgc
     4561 cgctgggcgt ggcgagaatg gtcgcagtgc cggctgtacc ggacatcgtg ggcaagatat
     4621 taccctacga gtgccggttg gaacccgtgc gattgataac gacacccaag aaattattgg
     4681 cgatttaacc cgccacggaa tgaaaatgct ggtggcaaaa ggtggttatc acggtttagg
     4741 taatacccgc tttaaatcct cagtaaaccg agctccacgc caaaaaacca acggtacgcc
     4801 tggcgaaaaa cgtgatttga aattagagct gttactgctt gccgatgtgg gaatgcttgg
     4861 cttaccaaac gccggtaaat ccacctttat ccgcagtgtc tctgctgcca aaccaaaagt
     4921 ggcggattac ccgttcacga cgctggtgcc aagtttaggg gtagcccgtg tgggggccga
     4981 ccgcagtttt gtggttgccg atattccggg gttaattgaa ggtgcagcag atggtgcagg
     5041 gctggggatt cgtttcctaa aacacttgga acgttgccgt attttgattc acttagtcga
     5101 tattatgccg attgatgaaa gcgatccggc tcataatatt tcggtgattg aatccgagct
     5161 ttaccaatac agcgaaaaac tcgccgaaaa accgatctgg ttggtgttta ataaaattga
     5221 caccatcggc gaagaagaag ctgctgagcg agcaaaagac attgccgagc aaatcggctg
     5281 ggaaggcgat tactacttga tttctgccgc taccggtcaa aacgtgcaag ctctcactcg
     5341 tgatattatg gactttattg aagccaaccc acgcgaagtg gaggaagaaa ataaagcacc
     5401 tgaagaagtc aaattcaaat gggaagacta ccacaacgaa gcaatgcaaa atgcggtaga
     5461 agaagattgg gacgacgatt ggagcgaaga agatgacgaa ggcatagagt ttatttacga
     5521 acgccattag tgcaataata caagcggttg tttttaaacg gagatttgca aatttccgga
     5581 gaaaaacaac cgcttgtagg tttaaggaga gtttatgctt agctcaacat cgtgcgattt
     5641 attcaatatt ccgttcttcc aatttgctca gatgaagaaa tattgccctg aggagatccc
     5701 tcgaattaag gcggaatata aaactcaatg ggaaatttgg aaaaagctac atcaggaagt
     5761 ttctcgtcag cttgggcagc cgtttgctcc accacatatt gagaagtggt gcaacggttg
     5821 gcaagttcga gcccattttt ttgcctatta caagtatgaa tttaatcgaa attctgcggc
     5881 tattctttcc gtgattttaa atcgtcgccg tttgcaagtg agccttgatt ggcattgtta
     5941 tcgagccgat cgctcgcaaa ttaacgtgca tcaatacaat caatggtggg aaaatttaga
     6001 tcgggaaaag tttgctgatt ttgagatttg gcacggaagt gagagtgaat atgatgattt
     6061 tagaaccgtt agtcagatgt cagagcaagc gttcacccta actaacgagg aagatttttg
     6121 gtgtatcggc aaaaatgtgg aaaaggacga tttggataac attgatgtag tagaatttat
     6181 ctaccaaacc attcgggcat tattgccact ttatgagaaa tgtcatcaat aaacactaaa
     6241 aagagaggcg aacatcattt gttcgcccct ttcaaaattt aagctatcac ttctttttta
     6301 ttttcaatgc gtaaacggta gcgtttatcc aacatacaga gtaaaccacc gagagccata
     6361 aacagcccgc caatccaaat ccagcgaatg aacggtttat aatacaaacg cactgcccaa
     6421 gagttatctt ctaatttttc gccaagtgca gcgtataaat ctcgggtaaa gccccagtca
     6481 atcgccgctt cggtcattcc cattcggctc accgtataga aacgtttttc agcattaagt
     6541 gtggcttcat atttaccgtt tcgagtaatt tcaagctcgg cagtgccgcc ttgatagttt
     6601 gccccatcgg tgattttaat acctttaaat tcaaattggt aatctaaaat ctcagcttta
     6661 tcacccacat tcattctgac atctcgttca atgctgaaat tttggctaaa tgcaatgccg
     6721 aacactgtca tcgcaacccc taagtgagcc agcaccattc cccaatgtga acgggacagt
     6781 ttgaaaatgc cacttaagaa cgagtggcga tgggttgcac gttggtgtag ctcatacagg
     6841 ctaagtaaca caataaagac agacatcatc gtgcctaaca ccacactcac ggtaatggtg
     6901 ttacggaata agtatggcaa tccgaatcca agcaatgcca ttaaaatagt tgcaataatt
     6961 accggtttac gaatagcaga aacttggtct ctacgccatt tcactaatgg tccaatccct
     7021 aatacaaatg agaaaggaac cattaaaatt aagaacattt ggtcaaaaaa tggtgccccg
     7081 attgaaatcg tccctaagcc gatttgtttg tgaattaatg gcagtaaggt ccctaaaaac
     7141 accaccgata aaaatgccat taataaaata ttattaatga gtagcatcgt ttctcttgaa
     7201 tagcgttcat agttatctaa gcttttaatt tccgagcctt tgaatgcata cagcagtaaa
     7261 gaaccaccta ctactaacac caagtaagcc aagatgtata aaccacgagt tggatctgaa
     7321 gcaaaggcgt gaactgacac caaaatgcct gaacgcacta agaaagtccc cactaaacag
     7381 agcgagaaag caagaatcgc taataagacc gtccacgctt taaatgtgcc tcgtttttca
     7441 gtcactgcta atgaatgaat cagtgctgtt cctgcgagcc acggcattaa tgaggcattc
     7501 tcgacaggat cccagaacca ccagccaccc caaccaagtt cgtagtatgc ccaccaagaa
     7561 ccaagcacga taccgagcgt taaaaatacc cacgctgcca ttgtccacgg acgagaccat
     7621 cttgcccacg cagtatctaa acgccctgtc atcagtgagg caatcgcaaa agcaaaggca
     7681 acggagaaac ccacatagcc catataaagc aacggtgggt ggaagatcaa gcccacatct
     7741 tgcagcattg ggtttaattc tttgccatca atcggaaaat ttgggaaagt tctatcaaac
     7801 gggttggagg taaaaatcac aaacatcaaa aagccgatgc caatgacccc cattagtcct
     7861 aacactcgag caacggcatc ttccggcatt ttacgggtaa aggttgctac cccgacagac
     7921 caaagcgaca acaaccaaac ccaaagcaat aatgagcctt cgtgagcgcc ccacaccgca
     7981 gataaacgat attgcaacgg taaacggcta ttggagttgt tcaccacata ttgcacggta
     8041 aaatcattca ccgcaaacag ataaaacagc gaaccaaaag agacagtcaa tgccaaaaac
     8101 attgcccaag tcatcgggcg ggcaagagca attaaggtgc tattttgctt ttctgctccc
     8161 caaagcggta aaactgccaa caagatagaa atcgctaggg ctaatgccaa cgtataattg
     8221 cctaattctg caatcatttg ctttcccctt gcacattgtt ggctttttct gcttccgcct
     8281 cggctttcat tttgcggtcg gtttccgatt cacctttcag atcttgttca gacacacctg
     8341 attttttatg ctgttctttt aatttatcgc ctaattccgg tggcatatag ttttcatcgt
     8401 gttttgccag aacttccgta gcttttaaac gggttggttc aatcaacacg ccttgtgcca
     8461 caatgccttg cccttcacgg aataaatccg gcaaaattcc ttcgtattcc acggtaatcg
     8521 aaggcccgat atcattgaga tcaaatacta cttttaaact tttatcatca cgctttaccg
     8581 tatcttccac taccattccg cccacacgga tacgctgacc aacttcaggc ttttgtttcg
     8641 gatcatcatt tttaccgttt acaatttcag acggcgtgta aaacagatcg atattttggc
     8701 ttaacgcata aagtgtcaag cctgctgcga cagaaagccc aagtaatatc gaaaccacca
     8761 cttttaatct tgatttgcgt cttggattca tcgtaatcac ccttcaaaat aaatgcaaaa
     8821 ttgcataaaa actcaaaaat aaattagtgt aagaatagca aaaaaccgca aaacattccc
     8881 gatatttcaa ggaagttttg cgatttctca aatcaaatac ctataaagaa gtatgaggct
     8941 atcgttgcaa aaaataaagt tatttcgacc gcttgtttat tgtgatgttt gctttaattt
     9001 ttcttcacgc acaatctctt tttttgcttg agccaaaatc gctttctgtt cacggcgact
     9061 aagccaaatc agcccaatca ttgcaatgaa actgacacca taagagagcc atacataaaa
     9121 gccgtagcca cccattgcaa aaaaatcgct gattgattca aattgaaatg tcattgttct
     9181 ctcctactct aaactgattt tgccagttgt tttacccaag ggcgattacg ttcgtccttt
     9241 aataaggcaa tgcgataacg ccaaatgcta aaccaagcgg taaaaattaa gctaccgaaa
     9301 atcgccaata gcaacggaat taacatttcc accgccatag acggtttatc gaatttagtg
     9361 atggtcgccc cttgatgcaa ggtgttccac cattccaccg aaaagtgaat aatcggcaag
     9421 ttaatcacgc caacaactga cagcacagcc gccgctttgg ctccggtttg tctgtcttgg
     9481 aatgcggaat aaagagccat cacgccgata taaaggaaaa ataacactaa agcggaggtt
     9541 aaacgagcgt cccacaccca ccaagtcccc cacattggtt tgccccaaat tgcaccggtt
     9601 gcaagtgaaa taaaagcgaa tactaagccg ataggtgcca ttgaaatcat cgccaagtta
     9661 gcttggcgaa tttgccaaac caatgccacc aagcctgcca ccgccattga gccatatacg
     9721 cccatcgacc aaattgctgc cggcacgtga ataaagataa tgcgatagct atcgccctgt
     9781 tggtaatctt tcggggcaaa agccaagccc caaacaaaag cagccgctaa cataatcaaa
     9841 cttagccaac ctaaaaacgg ttggattttc cctagcaaat ggtactgcgt ttccgatttt
     9901 gcataagggt gtagccattt ccacatatat ttctccaaag taaataattc ccctcacccg
     9961 tttacaggag agggacgatt tgagcttcgt tcagaagcga aaatcaggga gaggggaaaa
    10021 agttgcaaat tttcataata attcgaccgc ttcccctctc tctgaaaaac acctctgaac
    10081 gaggtgtttt tcggtctctc tccctcaaga gggagagagg aagttttagt tcaaactaat
    10141 tcttaatgca ccggcaatcg caaacggcga aaaagttaaa gtaaccgcta aaattgctcc
    10201 gataatcgcc agttgtccgc tataacccat atttaaggtg gcggcttcta ataccgcagc
    10261 ggcaaaaatc agtaccggta agaataacgg gaggattaat aaactcagca acgtaccgcc
    10321 cttgcgtaag cccacagtga gtgccacgcc aatcgctcct aagcagctta aaatcggggt
    10381 gccgagtaat aatgtcagca ctaacgccca ccaaacgtgg gtttcaagtg aaagcaaaat
    10441 ggcagcaatc ggtgaaagta aaatcaacgg taagccggtc agcagccagt gagcaatcac
    10501 tttcgctaac gcaacctgcg gcaaggcaag cggcagtaac attaattgct cgagcgaacc
    10561 gtctaaataa tcatcacgga ataaccgctc aaaggaaagc aatgcagaaa gcaaagcagc
    10621 tacccacgca atgccgccgg caattttgcc taataattcg gggttaggtc ccattaaaat
    10681 cgggaatacg gtaatcacca ttaaaaaaaa ccacaacgga tttaaaatct ccgagggttt
    10741 acgaaaggca atggtgagtt cccgctgaat aatagtcaat aaaatcatac tttaaattga
    10801 tctaacgaaa taatacgcac tttgtcgctt tcagcagcct ggtggctggt aaaaatcact
    10861 aatctgcctt gctcgcaatg ctgttcaata tgagcaatca gatccgccac acccttttta
    10921 tcaatagcgg taaagggttc atctaaaatc caaagtttgg cgttagtgag ccacaatttt
    10981 gccaacgcca cacggcgttg ctgaccggct gacaaatgcg aacaaggcaa atcttcacgc
    11041 ccgatgagcg acactttcgt caatgcgtgc caaagggcat catcatcttg cggtaactgt
    11101 tggattttct ggtagaaacg caaattttcc cacggtgtta attccggttt tacccccgaa
    11161 taatgcccta aataaaatag ttcagaataa tagtgatcac gctgtttttg aattgcaata
    11221 tcgttccaca gcacctcacc ttccgcaggt aaaaaaagcc cggctaaaat acgtaacagg
    11281 ctggtttttc caatgccgtt atgcccttca atctgcaccc attcaccact ttgcacggta
    11341 aaactgcacc cttcaaacaa acgggtttcg ccccgttcgc aggcaatctg attaagttta
    11401 agctgatatt gggccgtttg gttcattatt tttcatctgg ttgaacattt tagccgaagt
    11461 gtatcataag cctttgattt aacgtagcaa ataaattact tctttagagg tagcaagcgg
    11521 tcaaattttc aaaaagtttt gcaaattttg ataagaatca aaccgcttgt tacagagaga
    11581 ggatttcctt cacaaaagga atggttaatt tacgctgggc ttggagagac gctttatcca
    11641 acttatccaa tattttagag agttgctcta aatccgttcc cgcttttttt aacagaaaat
    11701 gagcgacctc atcggacaat tccacccctt tttggtgagc atttaactgt aaaatttggc
    11761 atttttgttc atcggttaaa tcgtttagct ggtacacttc gccccaagtt aagcgggaac
    11821 gtaaatccgg caattggata ttgagttggt gtggtgggca atcggcactg attaacagca
    11881 aggttttttg cccttcgtta aacaagcctt gttgttctcg aatttggtta aacaggttaa
    11941 aaatagcaag ttcccattca gcatctcctg caatcgcttg aatatcatct aggcaaatca
    12001 cattcagcag ttcggcatta tctaacacca acggcgagaa ataattggct ttagtgagcg
    12061 gaatataact agaagtttgg ttatttaaaa gaaagtggtt acttactgct ttcaacaaat
    12121 gacttttacc gcagctctta ccgccccaaa tgtaaaaaaa cggctgttga acatccgtaa
    12181 aatttcgttt gagggatcca agcaacactt cgctgttttg gctataaaaa ttgtcgaaag
    12241 tgtcttcatc aatttgatga atgggtagag gtaactgcaa ggctttcctt aaatctctaa
    12301 gtttgatatt tttctaagaa tagcggaaaa gcaggggttt gagaagtaaa aatagtgaga
    12361 aaatatccac tcatttttct aatgaagaga tgacaagcgg ttgaatttcc acataatttt
    12421 gtcaatctga ccgaaaaccc accgttttgt gttaaaaaaa gattgcagtt agctcaaaaa
    12481 attgtatatt caaagctttc tacttaaaaa gtagagtaaa acaactatta caaacacaac
    12541 ggagtgaata tgaaaaacat gactaaatta tcgctaataa cattagcgct agccgcttca
    12601 agtagcgtct ttgctgcacc gaaaaccttt gtttactgct tagaggcttc gccttcagca
    12661 ttaagcccag tgctatcaac agacggtgca agctttgatg tgaacgccct cccgatttat
    12721 aacaaattaa ccgactttga agacggtaca actaatgtta ttccctctct tgccgaaaaa
    12781 tgggacattt cggaagacgg caaaacttac accttccacc tgcgtaaagg cgtaaaattc
    12841 cacgacaata aagaatttaa accaagccgt gagttaaatg cagacgatgt agtgttctcc
    12901 attaaccgtc agtttgatcc aaaccatcca ttccacaaag tttccggcgg taactacgaa
    12961 tactttatcg gtatggatat gcaaaatatt atcgataaag tggaaaaagt ggacgatcac
    13021 accgttaaaa tcagcttaaa agtgccaaat gcgccgttct tagctaactt agcgatggat
    13081 ttcaacgccg tttactcagc ggaatatgca gagcaatcct taaaggccgg caagcctgag
    13141 cgtattgata ccaacccaat cggcagcggc ccgttccagt ttgtggatta ccaaaaagat
    13201 gccgccgttc gttacaaagc ctttgaaaac tactggcaag gcacagcgaa aattgaccgc
    13261 ttagtgtttg caattactcc ggatgcatcg gtacgtttag ccaagttaca aaaaggcgag
    13321 tgccacgcaa tgccttaccc taacccggct gatatcgaag atctgaaaaa agatccaaat
    13381 attgaattaa tgagccaatc tggaatgaat atcggctatt tggcattaaa ttcatcgatt
    13441 aaaccattag ataaccaaaa ggtacgtcaa gcattaaatc acgctgtaaa caaacaagcg
    13501 attgtagatg cggtatatca gggagccggt caagtagcga aaaacccaat tccaccaaca
    13561 atgtggagct ataatgaagc ggttcaagac tatgactaca accctgaaaa agccaaagca
    13621 ttgttaaaag aagccggttt tgaaaatggt tttgatactg aactttgggc aatgccggta
    13681 tcacgtccat ataacccgaa tgctcgccgt atggctgaac ttatccaaga agactggaaa
    13741 aaagtgggcg tgaatgcgaa aatcgtcagc tatgaatggg gcgaatatct caaacgctta
    13801 cgccaaggtg aagcggcaac ctcaatgatt ggctggacag gcgataacgg tgacccagat
    13861 aacttcctca acaccttatt aagctgctca gcggtggaag ccggttcaaa ctacgccaaa
    13921 ttctgccaca aaccgtttga agaagtcgtg cttggtgctg cccaagaaag cgacaaagca
    13981 aaacgtaccg agctttataa acaagctcaa gtgatcttca aagagcaagc tccttggatt
    14041 acgattgctc actccaccgt ttacttcccg gtgcgtaaag aagtaaaagg ttatgtgatg
    14101 agtccgttca gcttacataa cttctacaaa gtagatttag cggataaata atccgccaac
    14161 aagcggtaga atttgcagat ttttttacaa atttgaccgc ttgtcctccc ctctttgaac
    14221 tacgaggggc aggggggaaa tttggcagaa gcgattgccg aagtgagagg attgtaaaag
    14281 aggaaataaa ttttctgtct tattgattat tttcttcgtt aattactact gtcaaatctc
    14341 ccctaccccc tctttgctaa agaggggaat cagatcgaga gatcagacaa ctttgatgaa
    14401 aatccaacct aagagatttt aaatgttttc atttatttta aaacgtgtgc taatggtgat
    14461 ccctaccttt atagcgatta cgttaattac ttttgccctt gtgcatttta tccccggtga
    14521 tccaattgag atccgaatgg gggaacgtgg attatcgcct gaggttcatc aacaaatgat
    14581 ggaacaactc gggctgaacc gaccgttgtt tgaacaatac atcagctaca tcaaaggtgt
    14641 attgcacggt gatcttggta actcatttcg taacaacgaa ccggtgctga aggaattttt
    14701 taccctattc ccagccacgg ttgagttggc attttttgcc ctgctctggt cgttgatttt
    14761 cggcattatt ctcggtgtga ttgctgcggt gaaaaaagaa agttggattt cacacactgt
    14821 aaccacgctt tcactgaccg gctactcaat gccaattttc tggtggggct tgattttaat
    14881 tctttatttc tccaccccgc tcggcttacc ggcttcaggt cgtttaccat ctgaatactg
    14941 gattgaagcc gaaaccggtt ttatgctgat tgatacttgg aattcggacg aaccaggggc
    15001 atttttagcg gcaattaaat ctctgatttt gccgtctatc gtactgggga caatccctct
    15061 tgcagttgtg acccgaatga ctcgctcttc aatgttggaa gtgttgggtg aagactatat
    15121 ccgcaccgcc aaagccaaag gcttaaacac tacccgaatt gtgattgttc acgcattaag
    15181 aaatgccttg attccggtgg taacggtcgt tggcttaatt gtcgggcaac tgctttcagg
    15241 agccgtttta acggaaaaca tcttctcgtg gccgggtatc ggcaaatgga ttattgatgc
    15301 aatcaacagc cgtgattacc ctgtactaca aggctcagtg ctgattattg ctactattat
    15361 tattttagtg aaccttacgg tagatctact ctatggcatt gtcaatccga ggattcgaca
    15421 tagttagaaa aattccttcg atttttcccc tctttagcaa cgagggagtt acacaagcgg
    15481 ttcaaattcc gcaatttttt acaagattat ttttagttgg agttaatgat gtcaggaata
    15541 acggcggtaa atccacccgc acccaaaaca ccgttacagg agttttggta ctatttttgt
    15601 caaaatcggg gggcattaat aggacttacc tttattgcaa tcgtctttgt tgcgtgcgta
    15661 ttcgcacctt gggttgcccc ttttgatcca atcgaacaaa atcgcagtgc gttattgctc
    15721 cctcctgctt ggcttgaagg ggggaattcc gctcatctgc tgggtactga cgatattggg
    15781 cgtgatattc tttcccgtat tatttatggt tcaagattgt ccgtctttat cggcttagtc
    15841 attgtgctac tttcttgcgt attcggcgtg attttaggtc tacttgctgg ctactatggt
    15901 ggcacactcg acatcatcat tatgcgtttt gttgatatta tgctggcgat tccaagcctg
    15961 cttttaacta tcggcgtggt cactatttta ggcccttcat tgataaatgc cgctattgcg
    16021 attgcgattg tctccattcc aagttatgtt cgcttgaccc gtgcttctgt attaagcgag
    16081 aaaaaccgtg attatgttac tgcttctcgt gttgctggtg cgagcgtgct tcgcttaatg
    16141 tttgtggtga ttttacctaa ctgtttagct ccgcttatcg ttcagatgac gatggggatc
    16201 tcaaacgcta ttttagaact tgccgccctc ggcttcttag gcattggtgc acagccgcca
    16261 acaccggagc ttggcacaat gcttgccgaa tctcgtggct ttatgcaatc ggcaaactgg
    16321 ctggtgacca ttccgggtct agcgatttta tcgttagtat tagctttcaa cttaatgggt
    16381 gacggcttgc gtgacgccct cgatccaaaa ctgaaacaat aggagatcta aaatggcatt
    16441 attaacggtt gatcaacttt ccgtccattt tggtgaaact aaaacaccat ttaaagcggt
    16501 ggatcgtatc agttacgaag tcaatgaagg cgaagtatta ggcattgtgg gcgaatccgg
    16561 ttcaggaaaa tcggtaagtt ctctcgctat tatgggctta atcgatttcc ccggcagggt
    16621 tatggctgaa aaattgtatt ttaacggcaa tgatttattg gcattaaaac caaaagaaaa
    16681 acaacagatt gtaggggctg atgtctcaat gatcttccaa gatgcaatga ccagcttaaa
    16741 tccgagctat actgtgggct atcagattat ggaagcccta aaagtgcatc aaggtggctc
    16801 aaaagccagc cgtcgtgagc gagcgattga gttgctgacg atggtcggta ttcccgatcc
    16861 tgaatcccgt ttagaagttt atccgcacca gctttcgggt ggtatgagcc agagagtgat
    16921 gattgcgatg gcgattgcct gtcgcccgaa attattgatt gccgatgagc cgactactgc
    16981 gttagatgtg acgattcaag ctcaaatcat tgatttatta cttgaattac aacgcaaaga
    17041 aaatatggcg ttgattttaa tcacgcacga tttagcgtta gttgccgagt ctgcccatcg
    17101 cattatcgtg atgtatgcag ggcaagtcgt ggaagagggc aaagccgagc agatattcaa
    17161 agcaccactg cacccttaca cccaagcatt gctcaaagcc ttaccggaat ttgccgaagg
    17221 gaaatcccgc ctgcaatcac taccgggcgt agtgccgggc aaatatgacc gcccacaagg
    17281 ttgcttactc aaccctcgct gcccgtatgc aaccgaaaaa tgccgaacca ttgaaccggc
    17341 aatgcagata atcaacggca gacaggtgaa atgccacacg ccactgaatg cacagggtga
    17401 gccgataagg atagctgtat aacgtcataa agagtaggga cgcaatgctt cgcgtccgtt
    17461 acaagcggtg atttttaacc gatttttgca aaataacgat agtggacgca aagcattgcg
    17521 tccctacatt ttccgagaag gaaaacagaa tgaaagaaaa taattctctc ccaccattcc
    17581 tcaaagcagt ggacttaaaa aaatactacc cagttaaaaa gggaatgttc gctaaaccga
    17641 aaacggtaaa agcgctggat ggtgtctctt ttacccttga acgtggcaaa acccttgcga
    17701 ttgttggcga atccggctgt ggaaaatcaa ctttggggcg aatgctcact atgattgaaa
    17761 gcccaaccga aggtgaactg ttctacaacg ggcagaattt cttagtcaat gacaaagaaa
    17821 cggctcaact acgccgtaaa aaaatccaaa ttgtgtttca gaacccgtat agctcgctca
    17881 atccacgcaa aaaagtaggt agcattttag aagaaccatt attgattaac accaacttat
    17941 ccgcaaaaga acgcaaagaa aaagtgttgg aaatgatggc gaaagtcggc ttaaaaccgg
    18001 aattttacga ccgttatccg catatgtttt cgggcggaca acgccagcga attgcgattg
    18061 ctcggggctt aatgttgcag cctgatattg tggttgccga tgaacccgtt tccgcattag
    18121 atgtttcggt tcgggcgcaa gtgttgaatt tgatgatgga tttgcaagaa gaaatgggct
    18181 tgtcttacgt tttcatctcg cacgatttat ccgtagtgga acatattgcc gatgaagtga
    18241 tggtgatgta tttagggcgt tgcgtagaac aaggggatac caaaactatc ttcagcaatc
    18301 cacgtcaccc ttatacacaa gcgttactct ccgcaacccc tcgtttgaac ccggagcaac
    18361 gccgtgagcg gattaaactg actggcgaac taccaagccc gcttaatcca ccaaaaggct
    18421 gtgcctttaa cgcccgttgc agatttgcaa ccgatcgttg ctatgtggag cgaccagagc
    18481 tgaaaaccta tcaagacggt actcgcattg cttgttttgt ggtggaggaa aataattagg
    18541 caacgctcgc tatttaaaag gttaaagttt ttcaacttta acctttattt ttcatactta
    18601 atactcctca tcttttgtag caaaatcttt cattaatttg ctgatatgct cacaatggtg
    18661 tttaaatgac aatacgggca aataatcaaa tgcgttttaa cgccaatctt ctcttttaaa
    18721 gtgagttttc gttcctttga gtcagaaatt aaccttgtta cttgtcggca attcatctat
    18781 ttctctccgt taatttcctt gaaagacatt gttgtaactg cagccttgct cgatagatta
    18841 atgtatataa attatctgat gtaatattta ctactcgaca aatttcatct gaggttgcct
    18901 ctaaatattc cctcatcata aataccctag cttgttttgc aggtaaattg cttaagcaaa
    18961 cttcaaataa tatccaaaat tctttggaat aaacagcttc ttcctgccag ctagaaggat
    19021 aatattcttc tttccaatgc ccataattat caaaaaagtg attctcttcg tcatcatcag
    19081 ctagatca
//
LOCUS       DANLXF010000020        18970 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.020, whole genome shotgun sequence.
ACCESSION   DANLXF010000020 DANLXF010000000
VERSION     DANLXF010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 18970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18970
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.020"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(126..338)
                     /locus_tag="PXD66_000413"
     CDS             complement(126..338)
                     /locus_tag="PXD66_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318747.1"
                     /translation="MSIVIVDTDFVVHKEDEEWTTEEIKMIEEHLLEEQSAQYQVKRE
                     VPKQFYADIAERLSRGDLTAWEQLDG"
     gene            525..3110
                     /gene="leuS"
                     /locus_tag="PXD66_000414"
     CDS             525..3110
                     /gene="leuS"
                     /locus_tag="PXD66_000414"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDL6318748.1"
                     /translation="MQQQYNPSEIEPKVQRYWAENKVFKAVKDVTKEKYYCLSMLPYP
                     SGKLHMGHVRNYTIGDVVSRYQRMIGKNVLQPMGWDAFGLPAEGAAVKNNTAPAKWTY
                     ENIEYMKGQLKMLGFSYDWDREVTTCRPEYYKWEQWFFTELYKKGLVYKKTSTVNWCP
                     NDETVLANEQVHEGCCWRCDTPVEQREIPQWFIKITDYAEELLNQLDNLPQWPDQVKT
                     MQRNWIGRSEGVEITFKIAGSNVELPVYTTRPDTFFGVSYVAIAAAHPLAELAAQNNP
                     ALAEFIREAKNTKVAEAELATMEKKGMATGLFAIHPLTGKEVPVWVANFVLMHYGTGA
                     VMAVPAHDERDFEFAQKYDLQINQVIQPLDGSEWDFSKAAYTEHGKLINSAEFDGLEF
                     DVAFTAIADKLEAMGVGKRQVNFRLRDWGVSRQRYWGAPIPMMTTEEGEVVTVPLQDL
                     PVILPEDVVMNGVQSPIKADPEWAKTTYNGKPALRETDTFDTFMESSWYYARYTCPQY
                     HEGMLDSDEANYWLPVDQYIGGIEHATMHLLYFRFFHKLLRDAGILNSDEPATKLLCQ
                     GMVLADAFYYTSPTNERIWVSPTQVTLERDEKGRIIKATDPQGRELVHSGMTKMSKSK
                     NNGIDPQEMVEKYGADTVRLFMMFASPAEMTLEWQESGVEGAKRFLGRVWNLVYSYAQ
                     NPATTALDVNALDKDQKELRRDVHKTIAKVSDDIGRRQTFNTAIAAVMELMNKLTKAP
                     LETEQDKAVMAEALSAVVRMLYPITPHICFELWQVLGNEQTIDFAPWVQADESAMVED
                     EKLVVVQVNGKVRGKITVSATATEDEVKAIAKADPNVAKFLDGQQIVKEIYVPFKMLS
                     FAVKA"
     gene            3310..3798
                     /locus_tag="PXD66_000415"
     CDS             3310..3798
                     /locus_tag="PXD66_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318749.1"
                     /translation="MIKKLSLIAVFALTACGWHFKNNEVLPESFRTLTFESADQHSEM
                     SRILRNQLQLSDVKLVPSTANVAKLRLVSTSTDSKVVSVFKQAREAEKILTLNVDAIA
                     DIPNKGQYPLEVSVHRTFFDDSRAALAKSAEKEMIMKDMYEQASRQLIIKMAGLKKEL
                     SK"
     gene            3841..4866
                     /gene="holA"
                     /locus_tag="PXD66_000416"
     CDS             3841..4866
                     /gene="holA"
                     /locus_tag="PXD66_000416"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDL6318750.1"
                     /translation="MQKIFPETLQFSLNKGLQPFYLLTGNDLLLVNETKDAIIHTARL
                     NGFDEKKEVEIKNDTNWEELFEAAQSMGLFFARQIMVLNFPDSPTATQFKKLAELCAF
                     IHSDLLFILHLPKFSKTMEKQAWFTQIELNLVQINCQTPELSKLPQWVSHRAKAMNLQ
                     IENEANQLLCYSYEGNLLALKQALQLLQLNATDGKITLTKAQEVVEHSAQFTPFQWID
                     ALFEGKIQRAQRILYHLQNEEVQAVVLLRIVQKELMILLEITRNPTAVVSSEQSLFIG
                     NLRTEFDRLKIWQNRRPFYNQITQRLTYQKLYALIQQLAELERKVKQEFSDDIWQELE
                     RFSAQFT"
     gene            complement(4867..5052)
                     /gene="yacG"
                     /locus_tag="PXD66_000417"
     CDS             complement(4867..5052)
                     /gene="yacG"
                     /locus_tag="PXD66_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HDL6318751.1"
                     /translation="MPETIVNCPTCEKEVVWSKESKYRPFCSERCQIIDLGDWAAEKH
                     SIASEEAELFSEDLEKY"
     gene            complement(5034..5669)
                     /gene="coaE"
                     /locus_tag="PXD66_000418"
     CDS             complement(5034..5669)
                     /gene="coaE"
                     /locus_tag="PXD66_000418"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDL6318752.1"
                     /translation="MTYVVGLTGGIGSGKTTVSDLFSALGVEVIDADVVARQVVEPGT
                     PLLAKIVEHFGEEILTAEKALERTKLRQIVFNNEEEKTWLNNLLHPAIREEMVKKLQE
                     STACYVIWVVPLLIENKLTEYCDRILVVDVSPEIQLERATKRDNSKIETIKNIMAAQV
                     SREERLSYADDVIENNLPFEQGLLLIKEQVQALHYKYLALAKKEKECQKLL"
     gene            complement(5705..6361)
                     /locus_tag="PXD66_000419"
     CDS             complement(5705..6361)
                     /locus_tag="PXD66_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDL6318753.1"
                     /translation="MLILFSLFIAVWLKAEIPRFAIRVNRQIYQETTSLVNIGLTEQQ
                     FLAQSVLQPKQPRSANLFFLLFPLIMLCSESPIIGTIFILLCFLSLLDMCYYLTDIRY
                     LVLIFLLVLWESLADFQHQTFTFTLAFCLLIGLVSQLMFKKEAFGSGDMLLLVALSPL
                     FRLEKMLLLILTASLLGIGYYLAYWLMKKQKLAKLAFIPFISTAMLLVLMLESRGINY
                     "
     gene            complement(6364..7560)
                     /locus_tag="PXD66_000420"
     CDS             complement(6364..7560)
                     /locus_tag="PXD66_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDL6318754.1"
                     /translation="MTKIYEFHWQAQSRFGQKQRGKRLATNRDMLENLLLAQGYQHIR
                     ISRNFVLSANPKKEEVNSLLSQLALLVNSAMPLKQALSMLLQNCRNIRLYLWLNELIQ
                     HIESGYAFSQSLEKLGEFVTNQEIQLIKMGEQSGKLGIMLTNLAESRAKLEKLAKKVK
                     KILFYPVIVLLISLALSIGLLVLIVPQFAQLYGSKDKTLPLITDILFTLSECLIEQGT
                     VLLGTLLLAVIFYLFFAKKSRLVAKWKMALWAKMPLFSRIIEQSRIIFFSQNIALMLN
                     AHIPLDVALRSFLSEKNDDPILQQEIGTMLNLLQQGYKFSQGINPNVFGAEMAQMLEV
                     GEKSGNVAKMCEHISEMYQQKLDYQIDILSQLLEPMLMLIMGVIVGTIIVGLYLPIFD
                     MGALVE"
     gene            complement(7557..8009)
                     /locus_tag="PXD66_000421"
     CDS             complement(7557..8009)
                     /locus_tag="PXD66_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilB"
                     /protein_id="HDL6318755.1"
                     /translation="MEYSVTDLNSQRFIEISAEQWQKNCNEKQILLRYLAIPLQETEE
                     QLWLGIDDMQNLNACEIFAFIYHKQIEPVLISSDELKFLLNALSPEQSSRQSEIYEEN
                     TAYSLAHSEQVDRNDPIIQILENLFKFCLQHNASDIHFEPQKEKLLRQ"
     gene            complement(8030..8509)
                     /gene="ppdD"
                     /locus_tag="PXD66_000422"
     CDS             complement(8030..8509)
                     /gene="ppdD"
                     /locus_tag="PXD66_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HDL6318756.1"
                     /translation="MQKIVHFRPLGIRQLHRAFTLIELMIVIAIIAILATIAIPSYNS
                     YTQKAALSELLQASASYKSDVEVCIYNSGGLDNCSGGTNGIQANKTSSNTTKYLNSIA
                     VTKGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG"
     gene            8690..10069
                     /gene="radA"
                     /locus_tag="PXD66_000423"
     CDS             8690..10069
                     /gene="radA"
                     /locus_tag="PXD66_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDL6318757.1"
                     /translation="MAKAPKTAYVCNDCGAEYSRWMGQCKECKAWNTISEVRLISSKE
                     AAKSDRFSGYAGETSGKVQTLSDISLQEVPRFSSGFNELDRVLGGGVVPGSAILIGGH
                     PGAGKSTLLLQVMCGLAQHLPTLYVTGEESLQQVAMRANHLGLPTANLKMLSETSVEH
                     ICNIADQEKPKIMVIDSIQVMHLSDIQSSPGSVSQVRECASFLTRYAKTRQVAIIMVG
                     HVTKDGTLAGPKVLEHAIDASLLLEGESDSRFRTLRSQKNRFGAVNELGVFAMTEQGL
                     KEVKNPSAIFLSRSEEQTPGSSVMVLWEGTRPLLVEIQALVDHSMLANPRRVAVGLDH
                     NRLSLLLAVLHRHGGLQMSDQDVFVNVVGGVKVTETSADLALILALISSFRNRPLPQD
                     LVIFGEVGLAGEIRPVTSGQERISEAAKHGFKRAIIPHGNVPKKAIKDMQVFTVKKLS
                     DALDILYNL"
     gene            complement(10124..10354)
                     /locus_tag="PXD66_000424"
     CDS             complement(10124..10354)
                     /locus_tag="PXD66_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HDL6318758.1"
                     /translation="MDYVEILGYVAMILVACSFLLKDVIKLRVVNSLGCACFVVYGLL
                     IGSIPVTGLNALVVCINLYYISKGNRPTAAHS"
     gene            complement(10474..10968)
                     /gene="folA"
                     /locus_tag="PXD66_000425"
     CDS             complement(10474..10968)
                     /gene="folA"
                     /locus_tag="PXD66_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HDL6318759.1"
                     /translation="MKISIIVARTINKVIGKDNQMPWHLPVDLAWFRQNTLGKPVIMG
                     RKTYESIGRLLPKRPNIILSRSGFTVEGAYAVQSLEQAVELAKNFANTAEIMIIGGGE
                     LFKQALPLAERLYLTEIQADIEGDTFFEFDEQNWALVEEKWSEIDENNPFRCRFMCLE
                     RKSV"
     gene            11044..11199
                     /locus_tag="PXD66_000426"
     CDS             11044..11199
                     /locus_tag="PXD66_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl kinase"
                     /protein_id="HDL6318760.1"
                     /translation="MATLFLVTSEQIMSKTIVIKFGTSTLTHGTKSLSRPYMLELVKL
                     NYTNSIV"
     gene            11196..12164
                     /gene="proB"
                     /locus_tag="PXD66_000427"
     CDS             11196..12164
                     /gene="proB"
                     /locus_tag="PXD66_000427"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDL6318761.1"
                     /translation="MIVVTSGAAAAGRDYLGHPELPKTLASKQMLAAVGQSQLIRVWE
                     NLFDIYNIHIGQMLLTRADLDDRERFLNARDTLDALLAQKIIPVINENDAVATEEFKV
                     GDNDNLSALVAILAQADQLYLLTDQEGLFSADPRSNPDAKLLSVVEKITPEIRQIAGG
                     SSTGLGTGGMSTKITAADIATRSGVETIIASGSRPNVLVDLANGQNIGSKFLVQSDRL
                     EGRKRWLFAAPQVGEIVVDFGAEQALLQHKSLLPVGVSAIKGEFTRGEVIKIFSQDGK
                     ALALGIARYSSEALLRIKGKKSKEIESLLGYEFGSVAVHSDEMVVY"
     gene            12164..12928
                     /locus_tag="PXD66_000428"
     CDS             12164..12928
                     /locus_tag="PXD66_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HDL6318762.1"
                     /translation="MELGIDVLAILFAVATLAGFIDAIAGGGGLLTIPALMAAGLPPA
                     MALGTNKLQACGGSLSSSLYFIRKKAVNLKQIWLLILCTFLGSAAGTIFVQMINVDFL
                     KTLLPFLILIIGIYFLVSPKIGDEDRKQRISFTLFAFTAAVALGFYDGMFGPATGSFM
                     TLAFILLLGFNLSKAVAHAKVINFTSNFASLIFFTLGGAVMWKIGLIMMVGQFIGGTL
                     GAKMVMTKGKKLIRPMLVTMSFMMVGKMLYEQGIFN"
     gene            12931..13563
                     /gene="nth"
                     /locus_tag="PXD66_000429"
     CDS             12931..13563
                     /gene="nth"
                     /locus_tag="PXD66_000429"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005721589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDL6318763.1"
                     /translation="MNKAKRIEILTRLRNENPKPTTELNYSNPFELLIAVILSAQATD
                     KGVNKATDKLFPVANTPQAILDLGVDGLKEYIKTIGLFNSKAENIIKTCRDLIEKHNG
                     EVPEDRDALEALAGVGRKTANVVMNTAFGHPTIAVDTHIFRVSNRTNFAPGKNVVQVE
                     EKLLKVVPDEFKVDVHHWLILHGRYTCIARKPRCGSCIIEDLCEFKEKTE"
     gene            13760..15010
                     /locus_tag="PXD66_000430"
     CDS             13760..15010
                     /locus_tag="PXD66_000430"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HDL6318764.1"
                     /translation="MDIYLVGGAVRDQLLGLPVKDRDFLVVGATAEQLLAQGFQQVGA
                     DFPVFLHPETKEEYALARQERKNGNGYNGFICDFSPNVTLEQDLIRRDLTINAIAQDE
                     NSTLFDPYGGVQDLENRLLRHVSPAFSEDPLRVLRVARFAARFHSFGFTIAPETLKLM
                     REMVQSGELKHLTAERVWLETQKAFETDNPHVYFDILRLIGALKVLFPELDRLFGVPQ
                     PPQHHPEIDSGKHTLMVLQQAKRLAKKAENPTALLFAALCHDLGKGLTPADILPHHYG
                     HEVKGIQPTRELANRLKIPTEIKDFALLVTEYHTHCHKMAELRPETVIKLFNSLDVWR
                     KPNRFFDFLFACEADARGRLGFEQRNYPQPELAKKYYQAAIEIDVQQVIQDGFEKQAI
                     REELNKRRVYAVRSIKLDSDLINH"
     gene            complement(15111..16046)
                     /locus_tag="PXD66_000431"
     CDS             complement(15111..16046)
                     /locus_tag="PXD66_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDL6318765.1"
                     /translation="MSESKPFPITTGFFAMPLGLGALGLAWLNATPITPYATTIGNII
                     AGTSVILWVALLGCYVYRVFFCFEQIKAEFMHPVQCCLFSLVTMTTMISGDVLYVWDF
                     YLGELLIFLGVIVHLIFLVFIIGGLWKGTQFLPETLHPVHYLPPIAGSFTMASSLSLI
                     GYSEIASLFLGIGIISWLVYEPTMLQRIRLSPLFPIARPSLGIVLAPAFVGASAYFSM
                     GGELDLLAKMLWGYGFLQFCFILRNLKWIAENGFSMGFWAFSFGLAAMARGAIEFYQF
                     EPLSFIGLAGFTIANFGIGLLLIFTLKLWLQQSRA"
     gene            complement(16175..16369)
                     /gene="iscX"
                     /locus_tag="PXD66_000432"
     CDS             complement(16175..16369)
                     /gene="iscX"
                     /locus_tag="PXD66_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HDL6318766.1"
                     /translation="MKWTDTRMIAENLYDMNPDLDPTTVRFTDMHKWICEMEDFDDDP
                     EASNEHILEAILTIWLEEYE"
     gene            complement(16369..16710)
                     /gene="fdx"
                     /locus_tag="PXD66_000433"
     CDS             complement(16369..16710)
                     /gene="fdx"
                     /locus_tag="PXD66_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HDL6318767.1"
                     /translation="MPKIVFLPHEEFCPEGMVVEAKAGDNLLEVAHEAGVEIHHACDC
                     SCACTTCHVVIREGFDSLNDTSDQEEDMLDKAWGLEIDSRLSCQCIVGEEDLVVEIPK
                     YNLNHANEAAH"
     gene            complement(16729..17094)
                     /locus_tag="PXD66_000434"
     CDS             complement(16729..17094)
                     /locus_tag="PXD66_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 51 family protein"
                     /protein_id="HDL6318768.1"
                     /translation="MSNYVKTEISEQFRTVAVEFDLEDNRIFELGERINTEYDVAYMN
                     GYNWAAFLRAYLSIYRPSLLELLEEDPDVGCYYVTYPLSEKSKEKARELKKIIVYLVE
                     NEEEILNFIGEHLDNIEWD"
     gene            complement(17104..18957)
                     /gene="hscA"
                     /locus_tag="PXD66_000435"
     CDS             complement(17104..18957)
                     /gene="hscA"
                     /locus_tag="PXD66_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HDL6318769.1"
                     /translation="MALLQISEPGQTAAPHQHKLAVGIDLGTTNSLVATVRSGQTQVL
                     LDEQERALVPSVVHYAETEKTVGIEAFAKAALDPKNTVISAKRLIGRSLADVQQRYQN
                     LPYQFVASENGLPLIQTAQGNKSPIEVSADILSHLAQFAEKRLAGELSGVVITVPAYF
                     DDAQRQSTKDAARLAGLNVLRLLNEPTAAAIAYGLESGQEGVIAVYDLGGGTFDLSIL
                     RLRKGVFEVLATAGDTALGGDDFDHLLAEWIAEQANYKPQTASEQRELLTLATQTKVA
                     LSQAVEFEVKFANWTGVVSRSQFNELIQPLVKRSLMTCRRALKDAGVEAEEVREVVMV
                     GGSTRVPFVREQVGEFFGKTPLTSIDPDKVVAIGAAVQADILVGNKPDSDMLLLDVVP
                     LSLGIETMGGLVEKIIPRNTTIPVARAQEFTTGKDGQTAMTIHVLQGERELVEDCRSL
                     GKITLRGIPAMAAGVAQVRVTYQVDADGLLNVTAMEKSTKVQASIQIKPSYGLTDEEV
                     TQMIKSSMANAKEDMEARQLVEQRVEADRVIDSVVTALKEDGSAVLSVEEFQAIEAQL
                     ARLIELKEGTDRFAIQQGIKEVDLATQEFAARRMNLSIQKALAGKKMDDLA"
BASE COUNT         5642 a         4161 c         3792 g         5375 t
ORIGIN      
        1 aatccttgcg aaatgcgaca cctgcttttg gataaaactg taaaccattg atgatattcg
       61 tttgagtatg ctccgaaaat aatccataga aaataaactc taatagatta tttttaaaat
      121 caacactaac catctaactg ctcccaagca gttaaatctc ctcgtgataa acgttcagca
      181 atatcagcat aaaattgttt cggtacttcc ctttttactt ggtattgagc tgattgttct
      241 tctaataaat gttcttctat catcttgatt tcttctgttg tccactcttc atcttcttta
      301 tgtacaacaa aatcggtatc tacaatcaca atagacatac ctagctcctt caagtactgg
      361 ttatatattc tgtattttgc ctgttctaaa ttcagctgtc tagcttttct gttactcaca
      421 atttgcaaat ttttcatcaa attccaccgc ttgccattca aaaaaaggcg tttttgcggt
      481 atcctatcgg caattttttc agttaaattt aaaggttaaa ttttatgcaa cagcagtata
      541 acccaagtga gattgaacct aaggttcagc gttattgggc agaaaataaa gtttttaaag
      601 cagttaaaga cgttaccaaa gagaaatact attgcctttc aatgttgcct tatccgtcag
      661 gtaagctaca tatggggcac gttcgtaact acaccattgg tgatgtagtg tctcgttatc
      721 agcgtatgat cggtaaaaat gtattacagc cgatgggttg ggacgctttc ggtttaccgg
      781 cggaaggtgc tgcggtgaaa aataataccg caccggcaaa atggacttac gaaaacatcg
      841 aatatatgaa aggtcagctt aaaatgttgg gcttctctta tgactgggat cgtgaagtca
      901 ccacttgccg tccggaatac tacaaatggg agcaatggtt cttcacagaa ctttataaaa
      961 aaggcttagt gtataaaaaa acctcaaccg taaactggtg tccgaatgat gaaactgtgc
     1021 ttgccaacga gcaagttcac gaaggttgtt gctggcgttg tgacacccca gtggaacagc
     1081 gtgaaattcc acaatggttt atcaaaatca ctgactatgc tgaagaatta ctcaatcagt
     1141 tagataatct cccacagtgg ccggatcaag taaaaaccat gcaacgcaac tggatcggtc
     1201 gttccgaagg ggtagaaatc acctttaaaa ttgccggttc aaacgtagaa ttaccggttt
     1261 acactacccg tccggatacc ttcttcggcg taagctatgt ggcgattgct gcggctcacc
     1321 cattagcaga attagctgca caaaataacc cggcattggc tgaatttatc cgtgaagcga
     1381 aaaacaccaa agtggcagaa gcagaattag ccaccatgga gaaaaaagga atggcaaccg
     1441 gcttgttcgc cattcacccg ttaacaggaa aagaagtacc ggtttgggtt gctaactttg
     1501 tgttaatgca ctacggtaca ggtgcggtaa tggcggttcc ggctcacgat gagcgtgact
     1561 ttgaatttgc acaaaaatac gatttgcaaa ttaatcaggt aattcaaccg cttgatggct
     1621 cagagtggga tttcagcaaa gccgcttaca ccgaacacgg taaattaatc aactctgccg
     1681 agtttgacgg cttggaattt gatgtggctt tcactgcgat tgccgataaa ttagaggcaa
     1741 tgggtgtagg taaacgccaa gtgaatttcc gtctgcgtga ctggggtgtt tctcgtcaac
     1801 gttattgggg tgcaccaatc ccaatgatga ccacagaaga gggcgaagtg gttaccgttc
     1861 cattacaaga tttaccggtg attctccctg aagatgtggt aatgaacggc gtgcaaagcc
     1921 caatcaaagc cgacccagag tgggcgaaaa ccacttacaa cggcaaaccg gcattgcgtg
     1981 aaaccgatac cttcgatacc tttatggagt cctcttggta ttatgcacgt tatacttgcc
     2041 cacaatatca cgaaggaatg ttagattcag acgaggcaaa ctactggcta ccggtcgatc
     2101 aatatatcgg cggtattgaa cacgccacaa tgcacttgct ctacttccgt ttcttccata
     2161 aattgttgcg tgatgcgggt attttaaatt cggacgaacc ggcgactaaa ctgttatgcc
     2221 aaggtatggt gcttgccgat gcgttctact acaccagccc aactaacgag cgtatttggg
     2281 tcagcccgac acaagtcacc ttagaacgtg atgagaaagg tcgtatcatc aaagcaaccg
     2341 atccacaagg gcgtgagctg gttcacagcg gtatgactaa aatgtcgaaa tcaaagaaca
     2401 acggtatcga tccgcaagaa atggtggaaa aatatggtgc tgacacagtt cgcttgttta
     2461 tgatgtttgc ctcccctgcg gaaatgacat tagaatggca agaatccggc gttgaagggg
     2521 caaaacgttt cttaggtcgt gtttggaatt tagtttatag ctatgcccaa aatccggcaa
     2581 cgactgcatt agatgtgaat gccttagaca aggatcaaaa agaacttcgc cgtgatgtac
     2641 acaaaacgat tgctaaggtg agtgatgata ttggtcgtcg tcaaaccttc aatacagcga
     2701 ttgctgcggt gatggaattg atgaacaagc tcaccaaagc cccacttgaa accgaacaag
     2761 ataaagccgt aatggcagaa gctttaagtg cggtggtgcg tatgctttac ccaatcacgc
     2821 cacatatctg ctttgagtta tggcaagtgt taggtaatga acaaaccatc gattttgccc
     2881 cttgggtaca agccgatgaa tccgcaatgg tggaagatga aaaattagtg gtagtacaag
     2941 tcaacggaaa agtgcgtggc aaaatcactg tttcagctac tgctacggaa gatgaagtta
     3001 aagctatcgc taaagccgat ccaaacgttg ctaaattctt agatggtcag cagattgtaa
     3061 aagaaatcta cgttccattt aaaatgttga gctttgcggt taaggcgtaa caaacacact
     3121 ctcccttata tgggagagag accgataaat atttcgtgca gaaatattta tcagagagag
     3181 ggtacaagcg gttattttct tggaaaattt tgcaaaaaat agccccctct ccctgctttt
     3241 tcttctaaac gaagaaaaac ctgtccctct cccacaaggg gagaggggaa taaacaagga
     3301 aatctcatta tgattaaaaa actctctctc atcgctgttt ttgctcttac cgcctgcggt
     3361 tggcacttta aaaacaatga ggtattacct gaatcgttca gaacattaac ctttgaaagt
     3421 gcagatcaac acagcgaaat gtctcgaatt ttacgcaatc agttacaact aagcgatgta
     3481 aaattagtgc ctagcacggc taatgtggca aaattacgtt tggtttcaac ctcaacagac
     3541 agcaaagtgg tttctgtgtt taaacaagct cgtgaagcag aaaaaatctt aacgctaaac
     3601 gttgatgcga ttgccgatat tccaaataaa gggcaatacc ctttagaagt ttcggttcat
     3661 cgtactttct ttgatgactc tcgtgcagct ttggcaaagt ctgctgaaaa agagatgatt
     3721 atgaaagata tgtatgaaca agcctctcgc caattaatta tcaaaatggc gggcttaaaa
     3781 aaagaactct caaaataatc attgtagggg cgaaatatgt ttcgcccatt gtttatctgt
     3841 atgcaaaaaa tctttccgga aacacttcaa ttttcattaa acaaaggact acagcccttt
     3901 tatctattaa cagggaatga tctgctattg gtgaatgaaa ccaaagatgc cattattcac
     3961 accgcccgtc tcaacggttt tgatgaaaaa aaagaagttg aaatcaaaaa cgatactaac
     4021 tgggaagaac ttttcgaagc agctcaatca atgggattgt tttttgcacg ccaaattatg
     4081 gtgctgaatt ttccggattc ccccacagcg acacagttta aaaaactggc tgagctttgt
     4141 gcctttattc atagtgattt gctctttatc ctgcacttac ctaaattttc caaaacaatg
     4201 gaaaaacagg cttggttcac gcaaattgag ctaaatctcg tgcaaattaa ctgccaaaca
     4261 ccggaactta gcaaacttcc acaatgggtc agtcatcgag caaaagcgat gaatttgcaa
     4321 attgaaaatg aagcaaacca actcctttgt tatagctacg aaggcaatct actggcgctt
     4381 aaacaggcat tacaactgct acaactcaat gcaactgatg gaaaaatcac gctgacaaaa
     4441 gcccaagaag tggttgaaca ctccgcccaa tttactccat ttcaatggat tgatgcatta
     4501 tttgaaggaa aaatccaaag agctcaacgc attttgtacc atctgcaaaa tgaagaagta
     4561 caagcggtcg ttttattaag aattgtgcaa aaagagttga tgattttact cgaaatcact
     4621 cgcaacccaa ccgcagtcgt ttccagcgaa caatcacttt ttatcggtaa tttacgaaca
     4681 gaatttgacc gcttgaaaat ttggcaaaac cgcagacctt tttacaacca gatcactcaa
     4741 cgactgactt accaaaaact ttacgcccta atccaacaac tggctgaatt agagcgtaag
     4801 gtgaaacaag aatttagcga tgatatttgg caagaattag agcgtttttc cgctcaattt
     4861 acctaactaa tacttttcta aatcttcgct aaacaactcc gcttcttcac tggcgataga
     4921 gtgcttttct gccgcccaat cgcctaaatc aataatttga caacgctcgc tacaaaacgg
     4981 gcggtattta ctttctttac tccacaccac ttctttttca caagtcgggc agtttacaat
     5041 agtttctggc attctttttc cttttttgct aatgctaagt atttataatg taaggcttga
     5101 acctgctctt taattaaaag caagccctgt tcaaatggca agttattttc aatcacatca
     5161 tcagcataac ttaatctttc ttcacggctg acttgggctg ccataatatt tttaatggtt
     5221 tcaattttac tgttatcacg tttggttgct ctttccagtt ggatttcagg agaaacatca
     5281 acaaccaata tacgatcaca atattcggtg agcttatttt caattaaaag cggcacgacc
     5341 caaatcacat aacaagcggt cgattcttgc aattttttta ccatttcttc tcgaatggcg
     5401 gggtgaagta aattattgag ccacgttttt tcctcttcat tgttaaacac aatttggcga
     5461 agtttagtgc gttctaatgc tttttcagcg gttaagatct cttcaccaaa atgctctaca
     5521 atcttggcta aaagcggtgt acccggttca acaacttggc gagccaccac atcagcatca
     5581 attacctcta ccccaagagc tgaaaataaa tcagacactg tggttttgcc actgcctatt
     5641 ccaccggtta agcctacaac gtaagtcata aaaattgatt cctcaaaata aaaacaaacc
     5701 aattctagta attaatccct ctgctttcaa gcattagcac cagcaacatc gctgtgctaa
     5761 taaacggaat aaacgccaat tttgctaatt tctgcttttt cattagccaa taagccaagt
     5821 aataaccaat ccctagcagg ctggcggtta atatcaataa gagcattttt tcgagccgaa
     5881 agagtgggga taacgcaacc aataacaaca tatcgcccga gccaaatgcc tcctttttaa
     5941 acataagctg tgaaaccaag ccaatcaaca ggcaaaaagc aagggtaaag gtaaaagttt
     6001 ggtgttggaa atcggctaaa ctctcccata acaccaacaa aaatataagc acgaggtagc
     6061 gaatatccgt taggtaatag cacatatcca gcagtgataa aaaacagagc agaataaaaa
     6121 tcgtgccgat aatagggctt tccgagcaga gcataatcaa tggaaaaagg agaaaaaata
     6181 gatttgccga tcggggttgt ttcggttgta gcaccgattg agccaaaaac tgttgctcgg
     6241 ttaagccaat attcactaat gaagttgttt cttggtaaat ttggcgattg actcgaatgg
     6301 caaatcgagg gatctccgct ttgagccaaa ccgcaatgaa gagggaaaac agtattaaca
     6361 tcgctactcc accaaagccc ccatatcaaa aatcggtaaa tacagcccaa caataatagt
     6421 gccgacaatc acgcccatta tcaacattag catcggttct aatagctgag acaaaatatc
     6481 aatttggtaa tctaattttt gttggtacat ctcactaata tgctcacaca ttttcgccac
     6541 attgccactt ttctccccaa cctcaagcat ttgtgccatc tcagcaccaa acacattcgg
     6601 gttgatccct tgtgaaaatt tgtagccttg ttgtaacaaa ttaagcatag tgccaatttc
     6661 ttgctgtaaa atcgggtcat catttttctc ggataaaaac gacctcaagg caacatcaag
     6721 cggaatatga gcattcagca ttaaggcaat attttggctg aaaaagataa tgcgagattg
     6781 ctcaataatt cggctaaaca gtggcatttt agcccataga gccattttcc actttgcgac
     6841 taacctgctc ttttttgcaa aaaacaggta aaaaatgacc gctagtagta acgtgccaag
     6901 taatactgtg ccttgctcaa tcaagcattc ggaaagggta aataaaatat cggtaatgag
     6961 gggtaaagtt ttgtcttttg agccataaag ttgggcaaac tgcggtacaa tcaaaaccaa
     7021 tagcccgata gataaagcga gcgaaatcag caacacaatc accgggtaaa acaggatttt
     7081 cttcactttt ttcgccaatt tttccaattt cgctcggctt tcagctaaat tagtcagcat
     7141 aatgcccagt tttccgcttt gttcgcccat tttgattagc tgaatttcct gattggtcac
     7201 aaattcaccc aatttctcca acgattgtga aaaagcatag ccactttcaa tatgctgaat
     7261 cagctcatta agccatagat aaaggcggat atttcggcag ttttgcaaca acatagataa
     7321 tgcctgtttt aacggcatgg cagaattaac caacaatgca agctgtgata gcaagctatt
     7381 cacctcttct tttttcgggt ttgctgaaag cacaaaattt cggctaatcc gaatatgctg
     7441 ataaccttgt gccagcaaaa ggttttccaa catgtctcga ttcgttgcca accgcttgcc
     7501 ccgctgtttt tgcccaaatc ggctctgggc ttgccaatga aattcataga ttttcgtcat
     7561 tgcctaagca atttctcttt ttgtggttca aaatgaatat ctgaggcgtt gtgttgcaaa
     7621 cagaacttaa ataaattctc taaaatttgg ataatcggat cgtttctatc cacttgttcc
     7681 gaatgagcca atgaatacgc cgtattctcc tcataaatct cactctgccg actactctgc
     7741 tcaggcgaaa gggcattgag taaaaacttc aattcatcgg aggaaatcaa aacaggctca
     7801 atctgtttgt ggtagataaa cgcaaaaatc tcacaggcat tgagattttg catatcatca
     7861 atccctagcc aaagctgttc ttctgtttct tgcagcggaa tggctaaata tcgcaataaa
     7921 atctgctttt cattgcaatt tttctgccac tgctctgctg aaatctcgat gaacctttgg
     7981 ctgtttaaat ccgttaccga atactccatt ttgatttcct cacctcaaat tagccgtttg
     8041 gtgtaacagt tgtgctacag aaattagctg gaaataggct cgtatcattg ccactacaac
     8101 ttgccgtcca actaatcgta ttatttgaga acgacggtgt taaggtatag ccataaccat
     8161 tcagagagcc tttgccggtt accgtaatca cgccttttgt taccgcaatt gagtttaggt
     8221 atttagtggt attactactg gttttattcg cctgaatccc attcgtgcca ccggagcagt
     8281 tatccaaccc gccactgttg taaatacaga cttcaacgtc tgatttatac gaagcagatg
     8341 cttgtaataa ctccgataac gccgcctttt gggtgtatga gttataggaa gggatcgcaa
     8401 tagtggcaag aatggcaata atcgcaatca cgatcatcaa ctcaatgagg gtaaatgccc
     8461 gatgcagttg gcgaatgcca agcggtctaa aatgtacaat tttttgcata taaattcctt
     8521 ataaattgca ccaacaaatt gtggcgaatc aagtgtgcaa attttttgcc tgctttgcga
     8581 ccggcaaatt ttaatttttc gacgcagatc gaaaaaataa aatttttatc tgtttatcca
     8641 tacagttttt tgttagaatt tgccaaattt actcaattag gaatcaagaa tggcaaaagc
     8701 acctaaaacc gcttatgttt gtaacgattg cggggctgaa tattcccgtt ggatggggca
     8761 atgtaaggaa tgtaaggctt ggaataccat cagtgaagtg cggttgattt ccagcaaaga
     8821 agcggcgaaa tctgaccgct ttagcggcta tgccggtgaa acgtcaggca aagtacaaac
     8881 gctgtctgac atcagcttgc aggaagtgcc tcgctttagc agtggcttta acgagctgga
     8941 tcgtgtactc ggtggcggcg tagtgccggg gtcagcaatt ctgatcggcg gtcacccggg
     9001 ggcggggaaa agtacgttat tactgcaagt aatgtgcggt ttagcccaac atctgccaac
     9061 gctgtatgtc accggtgaag agtcgttaca acaagttgca atgcgggcaa atcacttagg
     9121 cttaccaacg gcaaatctga aaatgttgtc tgaaacctct gtcgagcata tttgcaatat
     9181 tgcagatcaa gaaaagccca aaattatggt gattgactcg attcaggtga tgcacttgtc
     9241 ggatattcag tcctcacccg gcagtgtttc acaagtgcgt gaatgtgcat ctttccttac
     9301 ccgctacgcc aaaacccgcc aagtggcgat tattatggtt gggcacgtca ctaaagatgg
     9361 cacgcttgcc ggcccgaaag tgttggaaca cgccatcgat gcttccttgc tgttagaagg
     9421 cgaatccgat tcccgtttcc gtactttgcg tagccagaaa aatcgttttg gtgccgtcaa
     9481 tgaattgggc gtatttgcga tgaccgaaca agggctaaaa gaggttaaaa acccatctgc
     9541 gattttttta agccgtagcg aagagcaaac gccgggcagt tcggtgatgg tgttatggga
     9601 aggaacacgc ccattgttgg ttgaaattca agccttagtc gatcactcaa tgctggcaaa
     9661 ccctcgccgt gtggcggttg gcttagatca taaccgttta tcgctattac ttgcggtgct
     9721 acaccgacac ggtggcttgc agatgtcgga tcaagatgtg tttgtaaatg tggtcggtgg
     9781 ggtgaaagtc acggaaacca gtgccgattt agccttaatt ttggcgttaa tttccagctt
     9841 ccgtaaccgt ccattgccgc aagatttggt gatttttggg gaagtcggct tagcagggga
     9901 aatccgcccg gttaccagtg ggcaagagcg gatcagcgaa gccgcaaaac acggctttaa
     9961 acgagcgata attcctcacg gcaatgtccc gaaaaaggca attaaagata tgcaggtgtt
    10021 caccgtgaaa aaactcagtg atgcgttaga tattctgtat aatctctaaa atcaagaacc
    10081 gcttgtaagt ctgtcttaca agcggtctgt tttaggtaaa aatttacgaa tgtgcggcag
    10141 ttggtctatt gcctttggaa atatagtata aattaataca caccaccaac gcatttaagc
    10201 cggtgaccgg aatcgagcca atcagcaaac cataaacaac gaaacaggca caacccagcg
    10261 aattcaccac ccgcagttta atcacatctt ttaataaaaa agaacaggca actaaaatca
    10321 tcgccacata gccgagtatt tcaacatagt ccatttagaa cctcctcaaa taaaataaac
    10381 tgccgatatt ttaaaggctg tttttttatt tgtatctagc tctaaaaata gcaaaggttt
    10441 gcgttcacat ttttactttt aaccgcttag ttattaaaca gattttcgtt ctaaacacat
    10501 aaaacggcaa cggaaagggt tattttcatc aatttctgac cacttttctt ctactaacgc
    10561 ccaattttgt tcatcaaatt caaagaacgt atccccttca atgtctgctt gaatctcagt
    10621 taaataaagt ctttctgcta gtggtaaggc ttgcttaaac agttcccccc caccaataat
    10681 cattatttca gctgtatttg caaaattttt cgctaattca accgcttgtt cgagcgattg
    10741 taccgcataa gccccttcca ccgtaaaacc tgaacgggaa agaataatat tagggcgttt
    10801 tggcaataac ctgccgatag attcataggt tttccgcccc ataatcaccg gtttgccgag
    10861 cgtgttttgg cgaaaccagg ctaaatctac cggcaaatgc cacggcattt ggttatcttt
    10921 gccaatcacc ttgttgattg ttcgggcaac aataatgctg attttcattt tttctcctgt
    10981 tatgcaaatt ggtttgattt tatatcaaat taagcgtgat tttacaccct aaaagtgata
    11041 cttttggcaa cattattttt agttacaagt gaacagatta tgtcaaaaac tatcgtgata
    11101 aaatttggca ccagcactct cacacacggc actaaaagtt taagccgtcc ttatatgtta
    11161 gagctggtaa agctcaatta caccaacagc atcgtgtgat tgtggtgaca tccggagctg
    11221 ccgctgccgg tcgggactat ttaggacacc ctgaactccc taaaacctta gcctctaagc
    11281 aaatgttagc agcggtcggg caaagtcagc tcattcgagt gtgggaaaat ttattcgata
    11341 tttataatat ccatatcggg cagatgctct taacccgagc cgatctggac gatcgtgaac
    11401 gtttcctcaa tgcccgtgat acccttgatg ccctgcttgc acaaaaaatc attccggtga
    11461 ttaatgaaaa cgatgccgtt gctaccgaag aatttaaagt aggcgataac gataatttat
    11521 cggctttagt tgcgattttg gctcaagcag atcagctcta tttattaacc gaccaagaag
    11581 gcttattcag tgccgatcct cgctcaaacc ctgatgctaa attgctctcg gtggtggaaa
    11641 aaatcacgcc tgaaatccgc caaattgccg gtggtagttc aaccggttta ggcacgggcg
    11701 gaatgagtac caaaattact gctgctgata ttgctacccg ctccggtgtg gaaaccatca
    11761 tcgcctccgg ttctcgccca aatgtgttag ttgatttagc aaacgggcaa aacatcggca
    11821 gtaaattttt agtccaatct gaccgcttgg aaggcagaaa acgctggtta tttgctgctc
    11881 cgcaagtggg tgaaattgtg gtagatttcg gggcggaaca agccttgctt caacataaat
    11941 cattactgcc ggtgggagtt tctgccatca aaggcgaatt tactcgaggt gaagtaatca
    12001 aaattttcag ccaagacggc aaagccttag cactcggcat tgcccgttat agcagtgagg
    12061 cattgctgcg aattaaaggt aagaaatcaa aagaaattga atcgctcctt ggttatgaat
    12121 ttggttctgt tgcagtgcat agtgacgaaa tggtggtgta ttaatggaat tgggaattga
    12181 tgttttagca atcttgtttg ccgttgcgac ccttgccggc tttatcgatg ccatcgctgg
    12241 cggcggtggc ttactcacta ttcctgcatt aatggcagca gggcttcctc cggcaatggc
    12301 acttggcacg aataaattac aggcgtgtgg cggatcactc tcttcctcac tctattttat
    12361 tcgcaaaaaa gctgttaatt taaaacaaat ttggttgcta attctatgca cttttttagg
    12421 ctctgccgcc ggtactattt ttgtgcaaat gatcaatgtc gatttcttaa aaacattgtt
    12481 gcctttctta attctgatta tcggcatcta ttttttggtc agccctaaaa ttggcgatga
    12541 agatcgtaaa caacgcatta gctttaccct atttgccttt accgccgctg tcgctctcgg
    12601 tttttatgat ggaatgttcg gaccggcaac cggctctttt atgactctgg catttatttt
    12661 gctgctcggc ttcaacctct ctaaagccgt agcacacgcc aaagtgatta attttacctc
    12721 caactttgcc tcattaattt tctttacgct tggtggtgcg gtaatgtgga aaatcggctt
    12781 aattatgatg gtcgggcaat ttattggcgg cacactaggt gcgaaaatgg tgatgacaaa
    12841 aggcaaaaaa ttaatccgtc cgatgctagt gactatgtct tttatgatgg tcggcaaaat
    12901 gctctacgag caaggaattt ttaattaaca atgaataaag caaaacgtat tgagatttta
    12961 acccgtttac gcaatgaaaa tccaaaaccg acaactgaac tcaattacag taatccgttt
    13021 gaattattaa ttgccgtgat tctatccgcc caagcgaccg ataaaggcgt aaataaagcc
    13081 accgataagc tcttccctgt cgccaacacg ccacaagcga ttttagattt aggcgtagat
    13141 gggctaaaag agtacatcaa aactatcggc ttatttaaca gcaaggcaga aaatattatc
    13201 aagacctgcc gagatctgat cgaaaaacat aacggcgaag tgcctgaaga cagagatgcc
    13261 ctcgaagccc ttgccggtgt tggcagaaaa accgccaatg tggtgatgaa taccgcattc
    13321 ggacacccta cgattgcagt cgatacccat attttcaggg tgtcgaaccg cacgaacttc
    13381 gctcccggca aaaatgtagt tcaggtagaa gaaaaactgc tcaaagttgt gccggacgaa
    13441 tttaaagtcg atgttcacca ttggttgatt ttacacggac gctacacttg cattgcccgt
    13501 aagcctcgtt gtggttcttg tattattgaa gatttatgtg agtttaaaga gaagacggaa
    13561 taacattatc tggtggtatt taatgaataa ctcccctctt tagaaaagta ggggctgggg
    13621 gagatttgat actatttcaa catttcgtat gagcgtatag aaattctgcc aaatcttccc
    13681 tatcccctct ttgctaaaga ggggaactag atcgagcgta aaatataaca agcggtcatt
    13741 tttttgagat tttttgcaaa tggatattta tttagtcggt ggagcagtgc gggatcaact
    13801 tcttgggctg ccagtgaaag atagagattt tttagtggtt ggcgcaactg ccgaacaact
    13861 tttagcccaa ggatttcaac aagttggggc agattttccg gtgtttttac accctgaaac
    13921 caaagaagaa tatgcccttg ctcgccaaga acgcaagaac ggcaatggtt acaacggctt
    13981 tatttgcgat ttctccccaa acgtcacgct tgagcaagat ttgatccgcc gagatttaac
    14041 cattaacgcc atcgcccaag atgaaaacag cacacttttt gatccttatg gcggtgtgca
    14101 agatttagaa aatcggctac tccgccacgt ttctcctgca ttcagcgaag atccgctacg
    14161 ggtgctacgg gttgcccgct ttgccgcccg ttttcattca tttggtttta ccattgctcc
    14221 tgaaaccctg aaattaatgc gagagatggt acaaagcggt gaattgaaac atttaaccgc
    14281 tgaacgtgtg tggttggaaa cacagaaagc ctttgaaacc gataatccgc acgtttattt
    14341 tgacatttta cgcttaattg gggcattaaa agtgctattc cctgaactgg atcgtctgtt
    14401 tggcgtacca caaccacctc aacaccaccc tgaaattgac tccggcaaac acactctaat
    14461 ggtgctacaa caggcaaaaa gacttgcaaa aaaagcggaa aatccgaccg ctttactctt
    14521 tgcagccctt tgccacgact taggcaaagg cttaacgcct gctgatattt tgcctcatca
    14581 ctacggacac gaagtcaaag gcattcaacc aacccgagag ttagccaatc ggttaaaaat
    14641 cccaactgag attaaagatt ttgccctttt agtgaccgaa taccacacgc attgccataa
    14701 aatggcggaa cttcgccctg aaacggtaat aaaactgttt aacagcctag atgtgtggcg
    14761 taaaccaaac cgcttttttg atttcctgtt cgcctgtgaa gcggatgcta gagggcgatt
    14821 aggttttgaa cagcggaatt acccccaacc tgaattggcg aaaaaatatt accaagccgc
    14881 aatcgaaatt gatgtgcagc aagttattca agacggtttt gaaaaacaag caattcggga
    14941 agagctgaat aaacgcagag tgtatgcggt aagaagcatc aagctagata gcgacctaat
    15001 aaaccattag gttatccgcc actttctcaa aatcaattcc caaagttcaa gttggttaaa
    15061 tgtatggatt ctttaaaagg tggcgttatt ttagtgaatt tataatgttc ctatgctctt
    15121 gattgttgta gccacaattt cagggtaaag atcagcaata acccaatgcc gaaattggca
    15181 atagtgaagc cggctaaccc aataaaagat aaaggttcaa attgataaaa ttcaatcgca
    15241 cctcttgcca ttgcagctaa accaaatgaa aatgcccaga aacccatcga gaagccattt
    15301 tcagctatcc atttgaggtt tcgtaaaata aaacagaatt gtaagaatcc atatccccat
    15361 aacatttttg ccaacaagtc taattctcca cccatagaaa aataggcaga agcgcccaca
    15421 aatgccggag caagaacaat tcctaaagat ggtctagcaa tcggaaaaag cggactaagc
    15481 cgaattcttt gtaacatagt gggttcatat actaaccagg aaattatgcc aatgcctaaa
    15541 aataaagatg caatttcact gtagccaatt aagcttaatg aacttgccat agtgaagctc
    15601 cctgcaattg gcggtaggta atgtaccggg tgtagcgttt ccggcaaaaa ttgtgtgcct
    15661 ttccataatc caccaataat aaaaaccaag aaaattaaat gcacaatgac gcctaaaaaa
    15721 atcaatagtt cgcctaaata aaaatcccat acatagagca catcaccgct tatcatggtt
    15781 gtcattgtca ctaaggaaaa taaacaacat tgcactgggt gcataaattc tgctttaatc
    15841 tgctcaaaac aaaaaaacac acgatacaca taacagccta ataaggctac ccaaagtatg
    15901 actgacgttc cagcaatgat attaccaatt gttgtagcat aaggagtgat aggcgttgca
    15961 tttaaccagg caaggcctaa cgcccccagc cctaatggca ttgcaaaaaa gcctgtagtt
    16021 attggaaaag gtttagattc actcataaat ttatcctcat attgaaataa tgcataactg
    16081 tattcggctt tataacagtc atatgtaaaa aattttattt ttctaaccgc ttgtaataag
    16141 aaaaacctaa agcacttgct ttaggttcct actactattc atactcttct agccagatag
    16201 ttaaaatcgc ttcaagaata tgttcatttg atgcttccgg atcatcatcg aaatcttcca
    16261 tttcacaaat ccatttatgc atatctgtaa aacgaaccgt ggtcgggtct aaatccgggt
    16321 tcatatcata cagattttca gcgatcattc gggtatctgt ccatttcatt agtgcgctgc
    16381 ctcatttgcg tgatttaaat tatattttgg aatttcaaca accaaatctt cttcaccaac
    16441 aatacattgg caacttaaac ggctgtctat ttccaatccc caagctttat ccagcatatc
    16501 ttcttcttga tctgaagtat catttaacga atcaaaaccc tcacgaatta ccacgtggca
    16561 agtggtacaa gcacaagaac agtcgcaagc gtgatgaatt tcaacgcctg cttcgtgtgc
    16621 tacttctaat aaattatctc cggctttagc ttcaacaacc ataccttccg ggcaaaattc
    16681 ttcgtgtgga agaaaaacaa tttttggcat tttaaagttt ccttattact aatcccattc
    16741 aatattatcc agatgttcgc cgatgaagtt taaaatctct tcctcatttt caaccagata
    16801 aacaataatc ttctttaatt cccttgcttt ctctttactt ttctcggaaa gcggataggt
    16861 aacataataa catcctacgt ccggatcttc ttctaataac tctaataggc ttggacgata
    16921 aatactaaga taagcccgta aaaatgcagc ccagttataa ccgttcatat aggcaacatc
    16981 atattcagta ttaatccgct cgcccaactc aaaaatgcgg ttatcctcta aatcgaattc
    17041 aaccgcaacg gttctgaatt gttctgaaat ctcggttttt acatagttag acatactgcc
    17101 tccttatgct aaatcgtcca tttttttacc ggctaatgct ttttgaattg ataaattcat
    17161 acgccgtgct gcaaattctt gcgtagctaa atccacctct ttgatacctt gctgaattgc
    17221 aaagcgatca gtaccttctt ttaattcgat taaacgggct aattgtgctt caatcgcttg
    17281 gaactcctcc acactcaaca ctgcagagcc atcttctttc aatgccgtta ccacgctgtc
    17341 aatcactcgg tcggcttcta ctcgctgttc aactaactgg cgggcttcca tatcttcttt
    17401 ggcatttgcc attgaggatt taatcatctg cgtaacttct tcatcggtta agccataaga
    17461 cggtttgatt tgaattgaag cctgcacttt ggtcgatttt tccattgcag tcacgtttaa
    17521 taaaccgtct gcatcaactt ggtaagttac ccgaacctgt gcaacccctg ctgccattgc
    17581 cggaatgcca cgcaaagtga ttttaccgag agagcgacaa tcttcgacta attcacgctc
    17641 accttgtaaa acgtggattg tcatcgccgt ttgaccgtct ttaccggtag taaactcttg
    17701 agctcgagct actgggatag tagtgttgcg tggaataatt ttctccacca aaccacccat
    17761 cgtttcaata ccaagcgaaa gtggcacaac atcaagcaac aacatatcgg aatccggttt
    17821 gtttccaact aaaatatccg cctgcactgc cgcaccaatg gcaacaactt tatccgggtc
    17881 gatagaggtt aatggcgttt taccgaaaaa ctcgccgact tgttcacgca caaacggtac
    17941 acgggtcgaa ccgcctacca ttaccacttc acgaacctct tctgcttcaa ctccggcatc
    18001 tttcaaggct cggcgacaag tcatcagcga gcgttttaca agcggttgaa ttagctcatt
    18061 aaattgcgaa cggcttacta cgcctgtcca atttgcaaat ttcacttcaa attcaaccgc
    18121 ttgcgaaagt gcgactttgg tttgagttgc caaagttaat aattcacgct gctcgcttgc
    18181 cgtttgaggt ttgtaatttg cctgttcagc aatccattcc gctaataggt ggtcgaaatc
    18241 atcgccaccg agtgcggtat cacctgcggt tgctaatact tcaaacacac ctttgcgtaa
    18301 acgcaaaata gataaatcga aagtaccgcc acctaaatcg taaacggcaa tcacaccttc
    18361 ttgtccgctt tctaaaccat aagcaatcgc tgctgccgtt ggttcattga gcaaacgtaa
    18421 tacatttaaa cccgcaagac gtgcggcatc tttggtactt tggcgttgtg cgtcatcaaa
    18481 ataagccggt acggtaatta ccacgcctga aagctcaccg gctaaacgct tttcggcaaa
    18541 ttgtgccaag tggcttaaaa tatccgccga aacttcaatc gggcttttat taccttgcgc
    18601 tgtttggatt aaaggtaaac cgttttcgct cgcgacaaat tgataaggta aattttgata
    18661 acgttgctga acatctgcca gagaacgccc aatgagacgt ttagctgaaa tgacggtatt
    18721 tttcgggtca agtgcagctt tggcaaaggc ttcaatccca actgtttttt ctgtttccgc
    18781 ataatgcact actgacggca ctaatgctct ttcttgttca tcaagcaata cctgagtctg
    18841 cccgctacgc acagttgcca ctaatgaatt ggttgtacct agatcaattc ctaccgctaa
    18901 tttatgttga tgtggtgcag cggtttgacc gggttcagaa atttgtaata atgccattta
    18961 tttatctctt
//
LOCUS       DANLXF010000021        18967 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.021, whole genome shotgun sequence.
ACCESSION   DANLXF010000021 DANLXF010000000
VERSION     DANLXF010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 18967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18967
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.021"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            33..716
                     /locus_tag="PXD66_000436"
     CDS             33..716
                     /locus_tag="PXD66_000436"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011201466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDL6318770.1"
                     /translation="MQLVFIRHGFSEWNAKNLFTGWRDVNLTERGIEEAKSAGQKLKA
                     AGFEFDIAFTSVLTRAIKTCNIVLEESDQLWIPQVKNWRLNERHYGALQGLDKKETAE
                     KYGDEQVHIWRRSYDISPPDLDPQDPNSAHNDRRYANLPKDLIPNAENLKITLERVLP
                     FWEDHIAPALISGKRVLVTAHGNSLRALAKHIEGISDADIMDLEIPTGQPLVYELDDN
                     LKVISKRYL"
     gene            complement(853..1410)
                     /gene="frr"
                     /locus_tag="PXD66_000437"
     CDS             complement(853..1410)
                     /gene="frr"
                     /locus_tag="PXD66_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDL6318771.1"
                     /translation="MINEIKKDTQDRMEKSLEALKGHIAKIRTGRAQPSLLDGIQVEY
                     YGSATPLRQVANVVAEDARTLAVNVFDKSLISAVEKAILTSDLGLNPSSAGTTIRVPL
                     PPLTEERRRDLIKIVKAEGEQGKVAIRNVRRDANDQIKALLKDKEISENEQHKAEEDI
                     QKITDTFIKKVDEVLAAKEKELLDF"
     gene            complement(1462..2178)
                     /gene="pyrH"
                     /locus_tag="PXD66_000438"
     CDS             complement(1462..2178)
                     /gene="pyrH"
                     /locus_tag="PXD66_000438"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDL6318772.1"
                     /translation="MSKPIYKRILLKLSGEALQGSEGFGIDPSILDRMALEIKELLAE
                     GVEVGVVLGGGNLFRGAKLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAEVNAKLM
                     SAFQLNGICDTYNWSEAIKMLREKRVVIFSAGTGSPFFTTDSAACLRGIEIEADVVLK
                     ATKVDGVYDKDPAKYADAKLYNKLTYAQVIEQELQVMDLAAFTLARDHGMPIRVFNMG
                     KPGALRNVVFGTEEGTTISE"
     gene            2270..3832
                     /locus_tag="PXD66_000439"
     CDS             2270..3832
                     /locus_tag="PXD66_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDL6318773.1"
                     /translation="MKSKQLFGILVAISLATAASCLMLIGSGFFPNPSIFEVILGVFL
                     IIALASSKWTYYLLLLPISIAYAIYTPVGIKFGKPTYEYIASIFATDMMESKEFIAQL
                     PILNIAAGIAIVLAVIFYRKITNRYQIRFLRNKTFVISAFVLMLFSLAPFKFFQEFYN
                     SSLKVKKELEVLNNLTLDSQWGQSTLSSTSNYDDYVLIMGESARKDYHHAYGYPAANT
                     PFMSSAKGTLIDGLTSGGTNTIASLRLMLTKPDTEKWEANYGLTLIDLIKSTGIKTYW
                     ISNQGYLGNYDTPISSIGNKSDVKIFTKAGDSLNTNVSDFELLPHFAKVIEEPAQGKR
                     FIFLHLYGSHPITCDRLTDYPKMFDDDKLPKRYFNVNCYVSSIKKTDELLKRVYDLLV
                     KNQENHQRSFSMVYLSDHGLSHEVSKENIVIHNGKDRSKLHYDIPLFKISSDDTQRNE
                     YKAFKSGLNFTNGIANWIGIENPLLDKEINLFNEQNDKSDYGLKKIIETQSTVEDPAV
                     PIPESYLYKATN"
     gene            complement(3896..4747)
                     /gene="tsf"
                     /locus_tag="PXD66_000440"
     CDS             complement(3896..4747)
                     /gene="tsf"
                     /locus_tag="PXD66_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDL6318774.1"
                     /translation="MAEITASLVKELRERTGAGMMECKKALVEANGDIELAIDNMRKS
                     GQAKAAKKAGRVAAEGVILARVASGFGVLVEMNCETDFVAKDAGFLELANEVADFAVA
                     NKGTTIEALQAQFEEKRAALVAKIGENMNIRRVQYLEGEVIAQYLHGAKIGVLVAGAG
                     SEDELKKVAMHVAASKPEFVNPEDVSADVVAKEREIQIEIAMNSGKPKEIAEKMVEGR
                     MAKFTGEVSLTGQPFVMDPSQSVGQYLKSVNTSVSNFIRLEVGEGIEKVEEDFAAEVA
                     KITGGNA"
     gene            complement(4843..5562)
                     /gene="rpsB"
                     /locus_tag="PXD66_000441"
     CDS             complement(4843..5562)
                     /gene="rpsB"
                     /locus_tag="PXD66_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005645552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDL6318775.1"
                     /translation="MAQVSMRDMLNAGVHYGHQTRYWNPKMKPFIFGARNGVHVINLE
                     KTLPLFNEALAELTRIASNNGKVLFVGTKRAASDAVKAAAVESQQFYVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQTQDGTFDKITKKEALMRTRELEKLELSLGGIKDMNGLPDA
                     IFVIGADHEHIAIKEANNLGIPVFAIVDTNSTPDGVNYIIPGNDDATRAIQLYLDSAV
                     AAIKEGRGVETVEVAEEASAE"
     gene            5907..6935
                     /locus_tag="PXD66_000442"
     CDS             5907..6935
                     /locus_tag="PXD66_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL6318776.1"
                     /translation="MKKTLSALAIAAATFTSTSTLAANEVNVYSYRQPYLIEPMLKNF
                     EKDTGIKVNIIFADKGLVDRVKQEGELSPADVLLTVDISRVMEIVNADLAQKIDSKVL
                     EKNIPAQFRDSNDQWFGLTTRARVIYTSKDRVGKLPAGFDYLDLAKPEYKGKVCVRSG
                     KNSYNVSLFAAMIEHYGIEKTKAFLEGLKANLARKPQGGDRDQVKAIKEGICDYSIGN
                     SYYYGKMLDDEKQKSWAEAAIINFPSGEHGTHKNISGVVIAKHSPNKANAVKLIEYLS
                     GEKAQGLYAELNHEYPVKEGIEPSAIVKGWGTFKSDTIKLEDIAKNYEAALKLVDEVK
                     FDDFSEKK"
     gene            7058..8704
                     /locus_tag="PXD66_000443"
     CDS             7058..8704
                     /locus_tag="PXD66_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL6318777.1"
                     /translation="MRSQFCWKSIAALTTFIVLLPLIAICYHALQGDNENLAHLWQTM
                     LPIYLKNSTLLVLGTVFLALIFALPTAWIVANYRFWGQRTLQWLLCLPLAMPAYLIAY
                     LYTDLLDYSGAFQGWLRSIFGWTHPQDYWFPQIRTLYGACFVLALVLYPYIFLLVRVA
                     LLEQSENLTHSAKMLGASPSQLFRRITLPLIRPSVAVGIALVAMETLGDFGTVAYFAV
                     PTLTTAIYDSWLGYHDLGTASQIAIFMLLMIFLFLSLEQYSRRKQKSYQRGYEKKSAF
                     KFLTGWKLALALAWCWGLLALAFFIPFGRLLYWAYDYFEQAWNLDFVQFSLNSLKVSI
                     IASVITVLIALLLHFSKRLNTRKILVKNDRLSLQLASLGYAVPGTVLAIGLLSPLTFA
                     DHQLHAWLKSLELSPVGLIFSGSLFALVLAYTIRFLAMAIGSLETSLNKISPSLDMAS
                     QTLGKNGSAMLVKIHIPLIYKGILTAGLMVFIESMKELNASLLLRPFNFDTLATYVFT
                     FTSDEQLERAALPAIVLVLVGLLPVIWLTRSLISQSEKER"
     gene            8714..9724
                     /locus_tag="PXD66_000444"
     CDS             8714..9724
                     /locus_tag="PXD66_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL6318778.1"
                     /translation="MSVLTINHLNLNFGSTKVLQNLHLAVNQNEIICLLGASGCGKTT
                     LLKAIAGLLPIEQGDIHLAGECLKSKAVEERQIGLIFQDYALFPHLTVADNIQFGLSK
                     LPKVQQKAITEKMLSVVHLQGFESRFPHELSGGQQQRVAIARALACQPELLLLDEPFS
                     NIDSQTRYAMIQEIKQILKSQNVPAIFVTHSKEEAFAFADKIAVMDQGKIIQFGKPNE
                     LYHSPVNPFVADFLGGTNYLNCNVTEDNVLCSPIGEFRLFPEMNVQKGRYRWLLRPEQ
                     IILKPNELGLGKVVGKLFLGLYYRYQVEIKGIEIVTYQSQELKIGEQVEVGFQVREFI
                     LF"
     gene            9734..10360
                     /locus_tag="PXD66_000445"
     CDS             9734..10360
                     /locus_tag="PXD66_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HDL6318779.1"
                     /translation="MKIEVWSDYACPFCYIGKRHLEQALAQFEGEVEVVFRAFELDPH
                     ANGEPEGDIQQRLMRKYQKTAEQADEMIRYVEQAGKQAGLDLRYRTTQYTRTFEAHRL
                     AKFAESKDLGEAMVERLFKAYFTDNTILAKRTELISLALDIGLERDEIAQLLTGDDFG
                     HEVREDERVAHKYGIHSVPFFVINEKLGVSGAQPPEILLDAIKQALQK"
     gene            complement(10422..11912)
                     /locus_tag="PXD66_000446"
     CDS             complement(10422..11912)
                     /locus_tag="PXD66_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HDL6318780.1"
                     /translation="MSLYQIARTETGKVISLNAKMANRHGLIAGATGTGKTVTLRKLA
                     ESLSDDGVPVFLVDVKGDLSGSVQAGNFSGKIAERILQFNLGGEDYLKGYPVSFWDVF
                     GTSGIPLRITISEMGPMLLSRLLNLNDTQGGLLNLVFRVADDRGLLLIDLKDLRALLK
                     FVADNAKEFQLEYGHISAASVGAIQRALLRLENEGADNLFGEPALDLHDWIQTRNGRG
                     VINMLNAEKLINSPRVYSAFLLWFMAELFENLPEVGDPEKPTFVLFFDEAHLLFDDAP
                     KALVDKIEQVVRLIRSKGVGVYFVTQNPLDIPDSVLGQLGNRIQHALRAFTPRDQKAV
                     KSAAETFRANKEVNVVEAITQLGVGQALVSVLDEKGMPTPVEIAYIYPPKSQLAPLAT
                     GERDSLVKQDDLYHHYSQYVDNESAFELLNAKAEAIKAEQAAAEEANNDFFGGILSSI
                     FGSKKKKDQSITEQVVSQVAHSVGRNLRNQVTKQIMRGILGAITKK"
     gene            complement(11971..13206)
                     /gene="rhlB"
                     /locus_tag="PXD66_000447"
     CDS             complement(11971..13206)
                     /gene="rhlB"
                     /locus_tag="PXD66_000447"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HDL6318781.1"
                     /translation="MSNHLTEHRFIDFPIHANVLNALDSKGFEFCTPIQAKTFPITLK
                     GKDIAGQAQTGTGKTLAFLIATFNHLLNNEIETSKNQPRALILAPTRELAVQIGADAE
                     LFLKHSDLKLALAYGGDGYDKQVQEIEKGVDILVGTTGRVIDYVKQGIINLDKIQVIV
                     LDEADRMFDLGFIKDIRYLMRKAPKPSERLTLLFSATLSAKVRELAYEDMDNAEYIEI
                     EPLQKTGHRIKEELFYPSNEDKMALLLTLLEEEWPERCMIFANTKHKCEEIWQYLSAD
                     GHRVGMLTGDIPQKKRLSLLDNFTKGNLDILVVTDVAARGLHIPDVTHVFNYDLPDDK
                     EDYVHRIGRTGRAGESGHSISFACERYAVNLPAIEEYIGHQIPVSQYDKESLLTLPKV
                     NRSRSKKTFLAKTKKNSRN"
     gene            complement(13294..14016)
                     /gene="recO"
                     /locus_tag="PXD66_000448"
     CDS             complement(13294..14016)
                     /gene="recO"
                     /locus_tag="PXD66_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDL6318782.1"
                     /translation="MTIDQWQRAFVLHRREYSENSLLVEFFTEHHGRITLLAKGARRA
                     RSPLKAVLQPFTPLLLRWSGKGDLKTLTKAEPAALTLPMETLSLYSGFYINEVLARVL
                     ENQTAYPELFQHYLRCITELATQKQIEPILRTFEFHTLKALGYGVDFTHCAATGLPVD
                     PKMTYQFQENQGFIASLLQNNQSFLGSDLLAFAQLDFSEKSTQQAAKRFTRMALKPYL
                     GSQPLKSRELFQSILPNKLKSG"
     gene            complement(14246..15163)
                     /gene="era"
                     /locus_tag="PXD66_000449"
     CDS             complement(14246..15163)
                     /gene="era"
                     /locus_tag="PXD66_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013525392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDL6318783.1"
                     /translation="MTEQIQTPAKTYCGFIAIVGRPNVGKSTLLNKILGQKISITSRK
                     AQTTRHRIVGIHTEDQYQAIYVDTPGLHIEEKRAINRLMNRAASSAIGDVDLIIFVVE
                     GTKWTEDDEMVLNKLRSAKAPVLLAINKIDNIKEKEELLPHITELSQKFDFAEILPIS
                     AQRGKNVHILQKFVRQSLRQGEHHFPEEYVTDRSQRFMASEIIREKLMRFTGEELPYS
                     VTVEIEQFKMNERGVYEINGLILVERDGQKKMVIGAGGQKIKTIGIEARKDMERLFDN
                     QVHLELWVKVKAGWADDERALRSLGYMDE"
     gene            complement(15266..15940)
                     /gene="rnc"
                     /locus_tag="PXD66_000450"
     CDS             complement(15266..15940)
                     /gene="rnc"
                     /locus_tag="PXD66_000450"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDL6318784.1"
                     /translation="MQLERLQKKLGYQFHNLDYLIQALTHRSAATKNNERLEFLGDSI
                     LNFTIGKVLYDKFPKANEGELSRMRATLVREQTLAIIARKFDLGDYLKLGPGELKSGG
                     FRRESILSDCVEAIIAAIYLDQDMAKAMEKVEDWYSDLLKDISPGDSQKDPKTRLQEF
                     LQGRKLPLPEYDVLDIKGEAHNQTFRVTCKVINVEEVFIGIGTSRRKAEQNAAEKALA
                     VVKTKK"
     gene            complement(15986..16945)
                     /gene="lepB"
                     /locus_tag="PXD66_000451"
     CDS             complement(15986..16945)
                     /gene="lepB"
                     /locus_tag="PXD66_000451"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL6318785.1"
                     /translation="MAQFMPIIFLGIIYGIWQWLDSMQLPNTISIILVALVIICGGFW
                     AFYKFSADPRRKQAIVLQQKRLGRELTAEEIAEIQPKSAVGEFFASLFGILFFVTVLR
                     SFLFEPFQIPSGSMEPTLRVGDFLVVNKFSYGIKEPIWQNTLIETGKPERGDVIVFKA
                     PAQPHVDYIKRVVAVGGDKIKYDFATQQLTVTKANGETKVFEYSEGKPNPEFFYHGEM
                     QVERTEKGNVTHQILNHPMAFNYAPYFYQQEGQAEGEWVVPQGYYFVMGDNRDNSEDS
                     RFWGFVPERNIVGKATFIWLSLDKKANEFPTGLRFSRMFTSIN"
     gene            complement(17032..18825)
                     /gene="lepA"
                     /locus_tag="PXD66_000452"
     CDS             complement(17032..18825)
                     /gene="lepA"
                     /locus_tag="PXD66_000452"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDL6318786.1"
                     /translation="MQNIRNFSIIAHIDHGKSTLSDRLIQTCGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLNYKAKDGEIYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAIEIDLEVVPILNKIDLPAAEPERVAEEIEDIVGIDAIDAVR
                     CSAKTGLGIEDVLEEIVKKMPAPEGDAEAPLQALIIDSWFDNYLGVVSLVRVKNGVLK
                     KGDKIKVMSTGQSYNVDRLGIFTPKQVDTTELKTGEVGWVVCAIKDILGAPVGDTLTH
                     HHNPATEVLPGFKKVKPQVYAGLFPISSDDYEAFRDALGKLSLNDASLFYEPETSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVVQTSGETIYVDSPSKLPA
                     INNIAEIREPIAECNMLVPQEFLGNVITLCVEKRGVQTNMVYHGNQIALTYEIPMGEV
                     VLDFFDRLKSTSRGYASLDYGFKRFQTADMVRVDIMINGERVDALAIIVHRANAAYRG
                     RELVEKMRELIPRQQFDIAIQAAIGNHVIARSTVKQLRKNVLAKCYGGDVSRKKKLLQ
                     KQKEGKKRMKSLGNVEVPQEAFLAILHVGKD"
BASE COUNT         5521 a         4056 c         3498 g         5892 t
ORIGIN      
        1 ccattaattt tactcaaagc taaggagtca ttatgcaatt agtatttatc cgtcacggtt
       61 tcagtgaatg gaatgctaaa aacttattta ccggttggcg tgatgttaat ttaacagaac
      121 gtgggattga agaagcaaaa tctgccggtc aaaaattaaa agcagccggt tttgaatttg
      181 atattgcgtt tacttcggtt ttaacccgtg cgatcaaaac ttgtaacatc gtattagaag
      241 aatcagacca attatggatc cctcaagtga aaaactggcg tttaaacgag cgtcactacg
      301 gtgcattaca aggtttagac aaaaaagaaa ctgctgaaaa atatggtgat gaacaggttc
      361 acatctggcg tcgttcttat gacatctctc caccggattt agacccacag gatccaaact
      421 cagcacataa cgaccgccgt tatgctaact taccaaaaga tttaattcca aatgcagaaa
      481 acctcaaaat cacccttgag cgtgtgttac cattctggga agatcatatt gcacctgcat
      541 taatttcagg taaacgcgta ttagttactg ctcacggtaa ctcattacgt gcattagcaa
      601 aacacatcga aggcatttct gatgcagaca ttatggactt agaaatccca accggtcagc
      661 cattagtgta tgaactagat gacaacttaa aagtaatcag caaacgttac ttataattgg
      721 taaatttccg ataaaaaata accgcttgta gtgcaacaca ttacaagcgg ttattttttg
      781 ttaatttttt gcaaatggca aagaacaaaa ataaaggcga atcatacatt cgccttattg
      841 ttttaaagta gattagaaat ctaacaactc tttctctttt gctgctaata cttcatcaac
      901 ttttttaatg aaggtatcgg tgattttttg aatatcttct tcagctttgt gttgttcgtt
      961 ttcgctgatt tctttatctt ttaataacgc tttgatttgg tcattagcat cacgacgaac
     1021 attacgaatt gccactttgc cttgctcacc ttctgcttta acgattttga ttaaatcacg
     1081 acgacgttct tccgttaatg gtggaagcgg aacacgaatg gttgtgccgg cagaagatgg
     1141 gtttaaacct aaatctgagg ttaaaattgc tttttctacc gcagagatta aagatttatc
     1201 aaatacgttt accgctaaag tacgggcatc ttcagctacc acgttcgcta cttgacgtaa
     1261 tggagtcgca gagccgtaat attccacttg aataccgtct aataaagaag gttgggcacg
     1321 acctgtacga attttcgcaa tatgcccttt taaagcctca aggctttttt ccatacgatc
     1381 ttgggtatct ttcttgattt cgttaatcat tgatttttta tccttttatt tagtaactga
     1441 gagttaaaca gagaaaatta attattcaga aatggttgta ccttcttcag taccgaatac
     1501 cacattgcgt aacgcccccg gtttgcccat attaaagaca cgaattggca ttccgtggtc
     1561 acgagcaagc gtgaatgctg ctaagtccat tacttgtaat tcttgctcaa taacttgcgc
     1621 atacgttaat ttattatata gttttgcatc tgcgtatttt gctgggtctt tatcataaac
     1681 gccatcaact ttagttgctt ttaataccac atcagcttca atttcaatac cacgcaagca
     1741 agcagctgaa tcggtggtaa agaacgggct acctgtaccg gcagagaaaa tcaccacacg
     1801 tttttcacgc aacattttaa ttgcttcaga ccagttgtaa gtgtcgcaaa tcccatttaa
     1861 ttggaacgca gacattaatt tagcatttac ctctgcacgg tgtaaggcat cacgcatagc
     1921 caaaccgttc attacggtgg caagcatacc catatggtcg cccactacac ggttcattcc
     1981 tgctttggct aattttgcac cacggaataa gttaccgccg ccaagtacca caccaacctc
     2041 aacgccttca gcaagtaatt ctttaatctc taacgccata cgatccaaaa tagacgggtc
     2101 gataccaaaa ccttcactgc cttgtagggc ttcaccactt aatttgagaa gaattcgctt
     2161 ataaataggt ttgctcattt taattgcctc ttaactgtat ggactaaaca caaaattacg
     2221 ttattataga acaatccaat ataacgataa actaaaaata ttcactacaa tgaaatccaa
     2281 acaacttttc ggcattcttg tcgctatttc gctggcaact gctgctagct gtttaatgct
     2341 aatcggatca ggcttttttc caaatccgag tatctttgaa gtcattttgg gcgtattcct
     2401 catcatcgct cttgccagct ctaaatggac ttattattta ctcttactgc caatcagcat
     2461 cgcttatgca atttataccc cggttgggat aaaattcggt aaaccgactt acgagtatat
     2521 cgcctctatt tttgctaccg atatgatgga aagcaaagag tttattgctc aactgccgat
     2581 tttaaatatt gcggcgggca ttgcgattgt actagcggtc attttttatc gaaaaattac
     2641 caatcgctat caaatccgtt ttttacgcaa taaaacattc gtgatttcgg cttttgtatt
     2701 aatgctgttt tcccttgcac cgtttaaatt ttttcaggag ttctacaatt cgagcctaaa
     2761 ggtaaagaaa gaattggaag tgctgaacaa tttaacgcta gattcacaat ggggacaatc
     2821 aaccctcagc tcaacttcaa actacgatga ttatgtactg attatgggcg aaagtgcgag
     2881 aaaggattat caccacgcct atggctatcc ggctgccaat acgcctttta tgagttcggc
     2941 aaaaggaacc ttgattgacg gtttaacctc cggtggcaca aacactatcg cctcattacg
     3001 cttaatgctc actaagcccg atacggaaaa atgggaagca aattatgggt taaccttaat
     3061 tgatttaatc aaatctaccg gtatcaagac ctattggatc tcaaatcaag gctatttggg
     3121 caattacgat acgccgattt cctctatcgg taataaaagt gatgtcaaaa ttttcactaa
     3181 agccggcgat tcattaaata ccaatgtgag cgattttgaa ttgttaccgc attttgctaa
     3241 agtgattgaa gaacctgctc aagggaagcg tttcatcttt ctgcatttat atggctcgca
     3301 tcctattacc tgcgatcgtc tcaccgatta cccgaaaatg tttgacgatg acaaactgcc
     3361 aaaacgctac tttaatgtaa attgctacgt gtcatctatc aagaaaaccg atgaattgct
     3421 gaaacgggta tatgatttgc tagtgaaaaa ccaagaaaat caccagcgtt cattctcaat
     3481 ggtttatttg tctgatcacg gtttatcgca cgaggtttca aaagagaaca ttgttatcca
     3541 caatggaaag gatagaagta aattacacta cgatattccg ctgtttaaaa tttcaagcga
     3601 tgatacccaa cgtaatgagt ataaagcctt taaatccggc ttaaacttca ctaatggtat
     3661 tgcgaattgg attggtatcg aaaatccgtt attagataaa gaaattaatc tttttaacga
     3721 acaaaacgat aaaagtgatt atgggctaaa gaaaatcatt gaaacgcagt ctactgtaga
     3781 agatccagct gttcctattc ctgaaagcta cttatataaa gcgactaatt agcaaaagta
     3841 agtaacaaaa aagcaggctt taagcctgct tttttagttt ttagtatcag aaaaattaag
     3901 cgttaccgcc agtgattttc gctacttctg ctgcgaaatc ttcttctact ttctcaatac
     3961 cttcacctac ttctaaacga ataaagttag aaactgaagt atttactgat tttaaatatt
     4021 gacctacaga ttgagatgga tccattacaa acggctgacc ggttaatgaa acttcaccgg
     4081 tgaatttagc catacggcct tcaaccattt tttctgcgat ttcttttggt ttaccagaat
     4141 tcatcgcaat ttcaatttga atttcacgtt cttttgctac tacatcagca gaaacatctt
     4201 ctgggtttac aaattcaggt ttgcttgcag caacgtgcat tgctactttc ttaagctcat
     4261 cttcagagcc tgcacctgct actaatacac caattttcgc accgtgtaag tattgagcaa
     4321 taacttcgcc ttctaagtat tgaacacggc gaatgttcat attctcaccg attttcgcaa
     4381 ctaatgccgc acgtttttct tcaaattgtg cttgtaatgc ttcgatagtt gtacctttat
     4441 ttgcaactgc aaaatcagca acttcgttcg ctaattctaa gaaaccggca tcttttgcaa
     4501 cgaagtcagt ttcacagttc atttcaacta atacgccaaa acctgaagcg acacgagcaa
     4561 ggattacacc ttcagctgca acacgacctg cttttttagc cgctttcgct tgaccggatt
     4621 tacgcatatt atcgattgct aattcaatat caccgttcgc ttcaactaat gcttttttac
     4681 attccatcat acctgcgccg gtacgttcac gaagttcttt aactaatgat gctgtgattt
     4741 cagccatttt aaattcccct tatttgatat ggtaaaacag gctctcacct gcctagaaat
     4801 ttcattgttt agggcaggtt aaaacctgcc ccctcaactg aattattccg cagaagcttc
     4861 ttctgctact tcaacagttt caacaccgcg accttctttg attgcagcaa ctgctgaatc
     4921 taagtaaagt tggatagcac gtgttgcatc atcgttaccg ggaatgatgt agttaacgcc
     4981 atctggagtt gagttagtat caacaatcgc aaataccgga atacctaagt tgtttgcttc
     5041 tttaattgcg atatgttcgt gatcagcacc gataacaaaa attgcatccg gtaaaccgtt
     5101 catatctttg ataccgccta agcttaactc taatttttct aactcacgag tacgcattaa
     5161 cgcttctttt ttagtgattt tgtcaaaagt accgtcttga gtttgagttt ctaaatcttt
     5221 taagcgtttg attgattgac gaactgtttt ccagttagtc aacataccac ctaaccaacg
     5281 gtggtttacg tagaattgtt ggctctctac tgccgctgct ttaacagcat cacttgctgc
     5341 acgttttgta ccaacaaata atactttacc attgttacta gcaatgcgag ttaattccgc
     5401 taatgcttcg ttgaataaag gtaaagtttt ttctaagttg attacgtgaa caccattacg
     5461 agcaccaaag atgaatggtt tcatttttgg gttccagtaa cgtgtttggt gaccatagtg
     5521 aacgcctgcg ttaagcatat cgcgcataga aacttgtgcc ataagaattt tccttcaaag
     5581 ttgtttgttt tgccacttat gaacggcaaa acggggttta gcctccacat atcctagaaa
     5641 atcaatctgt taaagacacc ccaattccta gcttgtgata tgtgtgtgtt ttagtgttaa
     5701 aaaataaatg tgtacgtttt gcctgaatag acaaaaacgg ggcgtattat gccaaaaaac
     5761 aaacaatttg ccaattattt tattgctctc ttcgcattac taaatcaagc tcaaaaataa
     5821 aaaccgttct cattattgac taaaaaaatg gggaggttta gaatgtcttt cattctgtta
     5881 tgaaaacctg tctgaaggag actatcatga aaaaaacact ttctgcactt gctattgctg
     5941 cagccacttt tacatcaact tcaacgcttg ctgcaaatga agtgaatgtt tattcttacc
     6001 gccagcctta cttaattgag ccaatgctaa aaaactttga aaaagatact ggcattaaag
     6061 taaacatcat cttcgctgat aaagggttgg ttgatcgtgt aaaacaagaa ggtgaattaa
     6121 gtcctgctga tgtgctttta acagtagata tttcacgcgt aatggaaatt gttaacgctg
     6181 atcttgcaca aaaaattgac tctaaagtgt tagaaaaaaa tattcctgca caattccgtg
     6241 actctaacga ccaatggttt ggtttaacca cacgtgctcg tgttatctac acgtcaaaag
     6301 atcgtgtagg taaattacca gcaggctttg actacctaga tttagctaaa ccagagtata
     6361 aaggtaaagt ctgcgtgcgt tcaggcaaaa actcttacaa tgtctcacta ttcgcggcga
     6421 tgatcgaaca ttacggcatt gaaaaaacca aagcattctt agaaggctta aaagcgaact
     6481 tagcacgaaa accacaaggt ggtgaccgtg atcaagtaaa agcaattaaa gaaggcatct
     6541 gtgattactc tatcggtaac agctactact acggcaaaat gttagatgat gagaaacaaa
     6601 aatcgtgggc tgaagcagcg attattaatt tcccgagtgg tgaacacggc acgcacaaaa
     6661 acatcagcgg cgtagttatt gcaaaacact caccaaataa agcaaacgcg gtgaaattaa
     6721 ttgaatacct aagtggcgaa aaagcacaag gtttatatgc tgaacttaac cacgaatatc
     6781 cagtaaaaga aggcatcgag ccatcagcaa tcgtaaaagg ttggggtacg ttcaaatcag
     6841 ataccatcaa acttgaagat atcgcaaaaa attacgaagc cgcattaaaa ttagttgatg
     6901 aagtgaaatt tgacgatttc tctgagaaaa aataatcaac cgataagcgg tcatttttcc
     6961 acaaaaattt gcaaaatttt gttaaaatac aaccgcttgc aaaaggacgc attttgcgtc
     7021 cttttatttt tttatgaaat aacaggctta aagaaaaatg cgatcacaat tttgctggaa
     7081 aagtattgct gccctaacaa cttttatcgt tttgcttcct cttattgcaa tttgctatca
     7141 cgcattgcaa ggggataatg aaaaccttgc tcacctttgg caaacaatgt tgccaatcta
     7201 tcttaaaaac tcaactttat tagtattagg aaccgttttc cttgcactga tttttgctct
     7261 gcctaccgct tggattgtgg caaattaccg tttttggggg caaagaacct tacaatggtt
     7321 actttgtttg cctctcgcta tgcctgctta tttaattgcc tatttatata ccgatttatt
     7381 agattactca ggtgcttttc aaggctggct gcgtagtatt tttggctgga ctcatccgca
     7441 agattactgg tttcctcaaa ttcgtacatt gtatggtgct tgctttgtgc ttgcgttagt
     7501 actatatcca tacatttttt tattagtgcg agttgcctta ttagaacaat ctgaaaactt
     7561 aacacacagt gcaaaaatgc tcggggcttc gccctctcag ttgttccgtc gaattacgct
     7621 gccattaatt cgaccttctg ttgccgtagg cattgcatta gtggcaatgg aaacgcttgg
     7681 cgatttcggt actgtggcct attttgccgt gccaacactt actacggcaa tttatgattc
     7741 gtggctaggc tatcacgatc ttggtactgc cagccagatt gcgatcttta tgttgctaat
     7801 gattttcctg tttttaagtt tagagcaata tagtcgccgt aaacaaaaaa gctatcaacg
     7861 tggctatgaa aagaaatcag cctttaaatt tttaactggt tggaaattag cattagccct
     7921 tgcttggtgc tggggtttat tggcgttagc ctttttcatt ccgtttggca ggttgctcta
     7981 ttgggcatac gactatttcg agcaagcgtg gaatttagac tttgtccaat tttcgcttaa
     8041 tagtttgaaa gtatcgatca tcgcctccgt gataacggta ctgattgctc tacttctgca
     8101 tttttctaaa cgtttgaata cccgcaaaat tttagtcaaa aatgaccgct tgtcattaca
     8161 acttgcctca ctgggctatg cggttcctgg cacagtatta gcgatcggtt tactttcgcc
     8221 tttaacattt gccgatcatc aactccatgc ttggctaaaa agccttgaat tatcacccgt
     8281 tggattgatt ttttcaggct cactctttgc attagtatta gcttatacca tccgattttt
     8341 agcaatggca attggcagcc ttgaaacttc gctaaataaa atctctccat cgctggatat
     8401 ggcgagccaa acgcttggta aaaatggctc agcaatgtta gtaaaaattc atatcccttt
     8461 aatttataaa ggtattttaa ctgcaggatt aatggtcttt attgagagta tgaaagagct
     8521 aaatgcttct ctgctacttc gcccatttaa ttttgataca ctcgcaacct atgtgtttac
     8581 ctttacatca gacgagcagc ttgagcgtgc tgctctgcct gccatcgtat tggtgttagt
     8641 cggtttactg cctgttattt ggcttaccag atcccttatt tcgcaatctg aaaaagaaag
     8701 ataaaggaat tttatgtctg ttttaactat taatcatctc aacctcaatt tcggcagtac
     8761 aaaggtttta caaaatctcc atcttgctgt gaatcaaaat gaaattatct gtttgctagg
     8821 tgcaagtggc tgtgggaaaa ctacactgct caaagccatt gcaggtttat tgccaattga
     8881 gcagggagat attcatttag cgggtgaatg cctaaaaagt aaagccgtgg aagagcgaca
     8941 aatcgggctg attttccaag attacgctct ttttccacac ttgacggtag ccgataatat
     9001 tcaatttggc ttatccaaat tgccaaaagt acaacaaaaa gcaattaccg aaaaaatgct
     9061 ttcagttgtg catttacaag gctttgaaag ccgttttcca cacgaacttt cgggcgggca
     9121 acaacagcgt gtcgctattg cccgagcatt agcctgccag ccagaattgc tactgttaga
     9181 tgagccgttt tccaatattg acagccaaac tcgctatgca atgattcaag agatcaagca
     9241 aatcttaaaa agccaaaatg tgcccgctat ttttgtaacc catagcaaag aagaagcctt
     9301 tgcatttgcc gataaaattg ccgtgatgga tcaagggaaa attattcaat ttggtaagcc
     9361 aaacgagcta taccattctc cagttaatcc atttgtggca gattttttag gtggcacaaa
     9421 ctatcttaac tgtaatgtaa cggaagacaa cgtgttatgc agccctattg gcgagttccg
     9481 actcttccct gaaatgaacg ttcagaaagg gcgttaccgt tggcttcttc gcccagaaca
     9541 gattatctta aaacccaatg agctaggttt aggtaaagtg gtaggcaaac tgtttttagg
     9601 cctatactac cgctatcagg ttgaaattaa aggcattgag atagtaactt accaatctca
     9661 agagctgaaa attggcgaac aagtggaagt gggcttccaa gtaagagagt ttatcctatt
     9721 ttaaggagaa tatatgaaaa ttgaagtttg gtcagattat gcctgcccgt tttgttacat
     9781 cggcaaacgc cacttagagc aagccctagc ccaatttgaa ggcgaggttg aagtggtttt
     9841 ccgagccttt gagcttgatc cgcacgctaa cggcgaaccg gagggcgata ttcagcaacg
     9901 cctaatgcgg aaatatcaaa aaaccgcaga acaagctgat gaaatgattc gttatgtgga
     9961 acaagccggc aaacaagctg ggctagattt gcgttaccgc accacccaat acactcgcac
    10021 ctttgaagct catcgcttgg cgaaatttgc agaaagtaaa gacttaggcg aagcaatggt
    10081 tgaacgttta ttcaaagcct attttaccga taacactatt ctagctaaac gcacagagct
    10141 tatcagttta gccttagaca ttggcttaga gcgtgatgaa atcgctcaac tgctcacagg
    10201 tgatgatttc gggcacgaag tccgtgaaga tgagcgagtc gctcacaaat atggcattca
    10261 cagcgtaccg ttttttgtga tcaatgaaaa actcggggta tccggggctc aaccgccaga
    10321 aatattactt gacgctatca aacaggcatt gcaaaaataa tccgaaaaaa gaccgcttgt
    10381 taagctaaac aagcagtctt attcttctta taatttacaa tttatttttt ggtgatcgca
    10441 cctaaaatac cacgcataat ttgtttggtg acttggttgc gtaaatttct gccaactgaa
    10501 tgcgccactt ggctaaccac ttgttcggta atcgattgat cttttttctt cttcgagcca
    10561 aaaatggaag agagaatacc gccaaagaag tcattgtttg cttcttctgc cgcagcctgc
    10621 tctgctttaa ttgcttctgc tttcgcatta agtaattcaa acgcagattc attatcgaca
    10681 tattggctgt agtgatgata gagatcatct tgcttcacta aactatcacg ttcgcctgtg
    10741 gctaatggag ctaattggct ttttggtgga tagatatagg caatttctac aggcgttggc
    10801 ataccttttt catctagcac cgaaaccaac gcttgcccca caccaagttg cgtgatggct
    10861 tctaccacat ttacctcttt attcgctcgg aaagtttccg ctgctgattt taccgctttt
    10921 tgatcacgtg gcgtgaaagc acgcaaagcg tgttggatac gattgccaag ctgccctaat
    10981 actgaatcag gtatgtccaa cggattttgc gtaacaaaat aaacacccac cccttttgag
    11041 cgaattaaac gaaccacctg ttcaatttta tccaccaatg ccttaggggc atcgtcaaac
    11101 aataaatgag cttcatcaaa gaataaaaca aacgtcggtt tttctgggtc gccaacttct
    11161 ggcaaattct caaagagctc tgccataaac caaagcagga atgcactgta cactcttggt
    11221 gagttaatta atttttctgc attcaacata ttgattacac cacgcccatt acgggtttgg
    11281 atccaatcgt gcaaatctaa tgcaggctca ccaaataaat tatctgcccc ttcattttca
    11341 agccttaata acgcacgctg aatagctcca acgcttgctg cagaaatatg cccatattcc
    11401 agttggaatt ctttcgcatt atctgcgaca aatttaagca acgcacgcaa atcttttaaa
    11461 tcaattagca ataaacctct gtcatctgcc acacgaaaaa cgagatttaa caagcctcct
    11521 tgggtatcgt ttaaattgag taaacgagaa agtaacatcg gtcccatttc ggaaatagtg
    11581 attcgaagtg ggatacctga tgtaccaaat acgtcccaaa acgaaactgg atagcccttt
    11641 aagtaatctt ctccccctaa attaaactgt aaaatgcgtt ctgcaatttt tcctgaaaaa
    11701 ttacccgctt gtaccgagcc cgaaagatca ccttttacat ctactaaaaa tactggcacg
    11761 ccatcatcac tcaaagattc tgccaatttg cgtaaagtaa ccgttttacc tgtacccgta
    11821 gccccagcaa ttaaaccgtg ccgatttgcc attttggcat taagcgaaat aactttgcct
    11881 gtttctgttc gagcaatttg atacaaactc atctttcatt ctcttaaagg taaattaatt
    11941 ttttgttctc atcatataaa aatttatcac ttaattacgg ctattctttt tcgtttttgc
    12001 caagaacgtt tttttacttc ttgagcggtt cactttcggt aaagtcagta aggattcctt
    12061 gtcgtattga ctcacaggaa tctgatgccc aatatactct tcaattgcag gcaaattaac
    12121 cgcataacgc tcgcaagcaa aactaattga atggccgctc tcccctgctc gccccgttct
    12181 accaatacga tgaacgtaat cttctttatc atcaggcaga tcataattga aaacgtgcgt
    12241 gacatctgga atatgcaatc cacgagccgc aacatctgtt accactaaaa tatccaaatt
    12301 gcctttggtg aaattatcca gcaaagaaag gcgttttttc tgcggaatat cgccggttag
    12361 cattccaacc cgatgtccgt ctgccgataa atactgccag atctcttcgc atttatgctt
    12421 ggtgttggca aaaatcatac aacgctccgg ccactcttcc tcaagcaatg tcagcaacaa
    12481 tgccattttg tcttcattcg aggggtaaaa tagctcttct ttaatacgat gtccggtttt
    12541 ttgtagtggc tcaatttcaa tatattccgc attatccata tcttcatagg caagttcccg
    12601 cacttttgcc gaaagcgttg ccgaaaagag caaagttaaa cgttctgaag gcttaggtgc
    12661 ttttcgcatt aaataacgga tatctttaat aaaaccaaga tcaaacattc gatctgcttc
    12721 atctagcaca atcacttgaa ttttgtctaa attgataatg ccttgtttta catagtcaat
    12781 cactcgacct gttgtaccga caagaatatc tacccctttt tcaatttctt gaacttgttt
    12841 gtcgtaacca tcaccaccgt atgccaaagc tagttttaaa tcagaatgtt ttaaaaacag
    12901 ctcggcatct gccccaattt gaaccgccaa ttcacgggtt ggagcaagaa ttaatgctct
    12961 cggctgattt tttgacgttt cgatttcgtt attcaataaa tggttaaaag ttgcaatcaa
    13021 aaaggcaagc gttttccctg tacctgtttg agcttgccct gcaatatctt tgcctttcag
    13081 cgtaatcgga aaggtttttg cctgaatcgg cgtacaaaat tcaaagcctt tgctatcaag
    13141 ggcatttagt acattagcgt gaatcgggaa gtcaataaag cggtgttcgg taagatgatt
    13201 agacatagta tttccttgat aattgtgatg tgacaagcgg tcttttttac acattttttt
    13261 gcaaaatagc atattgggcg ttttttctca aatttaaccg cttttgagtt tattcggcaa
    13321 aatagattgg aaaagctctc ggcttttcag cggttgtgac cctaaatagg gctttagtgc
    13381 cattcgggta aaacgtttcg ctgcttgttg agtacttttt tctgaaaaat ccaactgagc
    13441 aaacgctaat aaatcactgc ctaaaaagct ctggttattc tgtaataatg aggcgataaa
    13501 gccctgattt tcctgaaatt gataggtcat tttcggatca actggcaaac cggttgccgc
    13561 acagtgcgta aaatccaccc cataacccaa tgcttttaac gtatgaaatt caaaggtgcg
    13621 taaaatcggc tcaatctgtt tttgtgtggc aagctcggta atgcaacgta aataatgctg
    13681 aaataattca gggtaagcgg tctgattttc caatactctt gcaagtacct cgttgatata
    13741 aaacccacta taaagtgaaa gcgtttccat cggcaacgtt aatgcagccg gttctgcttt
    13801 ggttaaagtt ttcagatccc ctttacccga ccaacgcaac aacaacggcg tgaacggctg
    13861 taaaaccgct ttcaacggcg agcgagcccg ccttgcacct ttagcaagta aagtaatccg
    13921 cccgtgatgt tcggtaaaaa actccactaa taaactgttt tcgctgtatt cacgccgatg
    13981 aaggacaaag gctcgttgcc attggtctat tgtcataatt tcttgttaat tatgaggttg
    14041 taatttactt ctgccaaatc tccccctccc ctctttgcta aagaggggag atcttgcaga
    14101 gtatatttta aagtcatttt cactatttaa ccttttatat agataggaag atgaatttaa
    14161 aatttctgga gaactagtcc ccctctttag aaaagagggg ttaggggaga tttgattacg
    14221 atattaatgc tataacggga gccaactact cgtccatata ccctaaactt ctcaatgctc
    14281 tttcatcatc cgcccaacca gcttttactt tcacccaaag ctcaaggtgc acttggttgt
    14341 caaataggcg ttccatatct tttcgagctt caatgccgat ggttttgatt ttttgccccc
    14401 ccgcaccaat aaccattttt ttctgaccat ctcgttctac taaaatcaag ccattgattt
    14461 catacacccc acgctcattc attttaaatt gttcgatttc taccgttacc gaataaggta
    14521 actcttcacc tgtaaaacgc attagttttt cacgaataat ttctgatgcc ataaagcgtt
    14581 gagagcggtc tgtcacatac tcttcaggaa agtggtgctc accttgacgc agtgattgac
    14641 ggacaaattt ctgtaaaata tgcacatttt taccccgttg tgccgaaatt ggcagaattt
    14701 cagcaaaatc aaatttttgt gaaagctcgg taatatgcgg cagaagttct tctttttcct
    14761 tgatgttatc aattttattg attgccaata aaacaggggc ttttgccgaa cgcaatttgt
    14821 ttaacaccat ttcatcatct tctgtccatt ttgtgccttc cacaacaaag atgatgagat
    14881 ctacatcgcc aatagcagaa cttgctgcac ggttcattaa acgattaatc gcacgttttt
    14941 cttctatgtg taagcctggg gtatctacat aaatcgcttg gtattgatct tcagtgtgaa
    15001 taccaacaat acgatggcga gtcgtttgag ctttgcgtga ggtaatggaa attttttgtc
    15061 ctaaaatctt atttaataat gttgatttcc ctacatttgg gcgaccaaca atcgcaataa
    15121 aaccacaata ggtttttgca ggagtttgaa tttgttctgt cattttgttt tcctttcatt
    15181 ctgtggaatg aatttgatgg tgcaagcgtc cacgcttgta ccatttaatt aaatctttag
    15241 cacaagcgag gacgcttgcg ctatcttact ttttcgtttt aaccaccgct aacgcttttt
    15301 ctgcggcatt ttgttcggct tttcggcggc tggtgccaat gccgataaac acttcctcga
    15361 cgttaatcac tttacaggtt acacggaaag tttggttatg agcttcgcct ttaatatcta
    15421 atacatcata ttcaggcaat ggcaattttc taccttgtaa aaactcttgc aggcgggttt
    15481 ttggatcttt ttgtgaatcg cctggactga tatctttaag caaatctgaa taccaatctt
    15541 ccaccttttc cattgctttt gccatatctt gatcgagata aatggccgca ataatcgctt
    15601 caacacagtc cgataaaatc gattctcggc gaaaaccacc actttttagc tctcctggac
    15661 ctaattttag gtagtcgcct aaatcaaatt tgcgagcaat aatcgctaga gtttgctcac
    15721 gcacaagagt tgctctcata cggctaagct caccttcatt cgcttttgga aatttgtcgt
    15781 ataatacttt accaatagta aaatttaaaa ttgaatcgcc tagaaattca agacgctcat
    15841 tgtttttggt tgccgcactg cggtgtgtta aggcctgaat gaggtaatca aggttatgaa
    15901 attgataacc taattttttc tgtaatcgtt ctagttgcat agtttatgtg aataaagggg
    15961 cgtatctcat acgcccctgt taagattaat tgattgaggt aaacattcta gaaaaacgaa
    16021 gccccgttgg gaattcattt gcttttttat ctaaacttaa ccaaataaaa gtggctttac
    16081 caacgatgtt tctctcaggc acaaagcccc agaaacgact gtcttcactg ttatcacggt
    16141 tatcacccat cacaaaatag tatccttgtg gcacaaccca ttcaccttct gcttgccctt
    16201 cttgttggta gaaataaggt gcatagttaa atgccattgg gtgatttaaa atttgatgag
    16261 ttacattacc tttttcagtg cgttctacct gcatttcacc gtgatagaaa aactcaggat
    16321 ttggcttgcc ttcgctgtat tcaaatacct ttgtttcgcc attggcttta gtaaccgtta
    16381 attgctgagt tgcaaaatca tatttaattt tatcaccacc tactgccacc acacgcttaa
    16441 tataatcaac gtgtggttgt gccggtgctt tgaatacaat cacatcgcct cgttctggtt
    16501 tgcccgtttc aatcaaggtg ttttgccaga taggctcttt aatgccgtag ctgaatttat
    16561 ttacgactaa aaaatcgcct acacgcaagg ttggctccat tgaaccactt ggaatttgaa
    16621 acggttcaaa taagaacgag cgaagtactg taacaaagaa taaaatgccg aaaagtgagg
    16681 caaaaaattc gcctactgcg gatttaggtt gaatttccgc tatttcttca gcggttaatt
    16741 ctctgcctaa acgtttttgt tgtagaacaa tcgcctgttt acgtcttggg tcagcactga
    16801 atttgtaaaa cgcccaaaat ccaccacaaa taataaccaa ggcaactaaa ataatggaaa
    16861 tagtatttgg cagttgcatt gaatcaagcc attgccaaat accataaata atacctagaa
    16921 aaataattgg cataaattgt gccactgaat gctccttttt attcaaaatt gcaaagtttt
    16981 ctcaaaatat gaccgcttgc aagcggtcgt attttacacg aatattgaca attaatcctt
    17041 acccacatga agaattgcca aaaatgcttc ttgtggcact tctacgttac cgagagattt
    17101 cattcgtttt ttaccctctt tttgtttttg tagcagtttc ttcttacgac ttacatcacc
    17161 accgtaacat tttgctaata cgtttttacg caactgtttt accgttgagc gagcaatgac
    17221 gtggttacca attgccgctt gaatagcgat gtcaaattgt tggcgtggaa taagttcacg
    17281 cattttttcc actaattcac gtccacgata cgccgcattt gcacggtgaa cgataatcgc
    17341 caacgcatca acacgctcgc cgttaatcat aatatccaca cgcaccatat cagccgtttg
    17401 gaaacgttta aaaccgtaat cgagtgaagc ataaccacga gaggttgatt ttaagcggtc
    17461 gaagaagtct aacactactt cgcccattgg gatttcgtaa gttaaggcga tttggttacc
    17521 gtgatagacc atattggtct gtacgccacg tttttccaca caaagggtaa tcacgttgcc
    17581 taggaattct tgtggcacaa gcatattaca ttctgcaatc ggctcacgaa tttctgcaat
    17641 attgttgatt gctgggagtt tcgatgggct atccacatag atcgtttcgc cactggtttg
    17701 caccacttcg taaactacgg tcggagcggt agtaatcaaa tcaagatcgt attcacgctc
    17761 taaacgctct tgaatgatct ccatatgcaa caagcctaag aagccacacc ggaagccaaa
    17821 gcccaacgca cttgaagttt ctggctcata gaacaatgac gcatcattta ggcttaattt
    17881 gcctaaagca tcacggaagg cttcgtagtc gtctgaacta atcggaaata accctgcata
    17941 aacctgtggt tttactttct taaagcccgg taatacttca gttgctgggt tgtggtggtg
    18001 ggttaatgta tcgcccactg gtgcccctaa aatatcttta atcgcacaaa ctacccaacc
    18061 tacttcacct gtttttagct cggtagtatc cacttgtttt ggcgtgaaaa taccaaggcg
    18121 atctacattg taagactgcc ctgtactcat cactttaatt ttatcgcctt ttttcagcac
    18181 gccatttttc acacgcacta aagacacaac gcctaaatag ttatcgaacc aagagtcgat
    18241 aattaacgct tgtaatggtg cttccgcatc gccttctggt gcaggcattt tcttcacgat
    18301 ttcttctaaa acatcttcaa tgcctaaacc tgttttagct gaacagcgta ctgcatcaat
    18361 ggcatcaatg ccgacaatat cttcaatctc ttctgccaca cgctcaggct cggctgctgg
    18421 taaatcgatt ttatttaaaa ttggcacaac ctctaaatct atctcaattg cggtataaca
    18481 attcgccaac gtctgtgctt ccaccccctg ccctgcatca accactaata aagcaccttc
    18541 acaagcagca agcgagcgag aaacttcata tgagaaatcc acgtgccctg gcgtatcaat
    18601 aaagttgagc tgataaattt cgccatcttt cgctttatag tttaaggtta cactttgtgc
    18661 tttaatagta attccacgct cacgctcaag atccattgaa tctaatactt gagcctccat
    18721 ttcacgatct gataagccac cacaagtttg aattaagcgg tctgataaag tcgatttgcc
    18781 gtgatcgata tgagcgataa tcgaaaagtt acgaatattt tgcattttca tcgggcagtt
    18841 aatgtcctgt tttgttattt gtttatcaaa aattaatcgg ggaattgtac ttgaaataca
    18901 aggttttgca aagaaaacaa atagcaataa gcggttattt tttcctaaaa atgtgcaaat
    18961 ttcttga
//
LOCUS       DANLXF010000022        18945 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.022, whole genome shotgun sequence.
ACCESSION   DANLXF010000022 DANLXF010000000
VERSION     DANLXF010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 18945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18945
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.022"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            7..219
                     /locus_tag="PXD66_000453"
     CDS             7..219
                     /locus_tag="PXD66_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL6318787.1"
                     /translation="MSAITGTVKWFNATKGFGFITPDQGGKDVFVHFSAIEANGGYRT
                     LEDGAKVQFEIQDSERGASAVNVKTI"
     gene            complement(279..1433)
                     /locus_tag="PXD66_000454"
     CDS             complement(279..1433)
                     /locus_tag="PXD66_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HDL6318788.1"
                     /translation="MMKVSPFQWAAFNYFGFFCAYGVLLPFLPVWLKHHGYSTEMIGL
                     FAAVGYLFRFAGSMLASQRVKTVSQLIPTARSLTWLNIAAAAALLFSGDFIWLLFPVL
                     MLFQMFNSGAMPIADSIASIWQHQIGLDYGRARLFGSMAFVVGSLSAGYLIGLLGENS
                     VVWIIIGFLVFLGIGQLATPKVGFEEQGNKANTSSVTYRQLLKEPETLKMLIAVSLIS
                     ASHAAYYTYSTIHWSNASISTAMTSLFWGLAVVAEILFFLVSKRLFKSWKISHLMILA
                     AVFAVIRWAIMGSTTEIVLIIVMQMLHSITFGAAHIAMIRYISMQSAERVTKLQGLYF
                     GLASCAFMAVFTFIAGMVYQHSPSLTFWLMAIFTLPAIFIVPKRFEVQIN"
     gene            complement(1430..2248)
                     /locus_tag="PXD66_000455"
     CDS             complement(1430..2248)
                     /locus_tag="PXD66_000455"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDL6318789.1"
                     /translation="MNEKKLAFIGTGNMAYAIIQGLLKSGYSAEQIIACNKSNLARRE
                     ELQAVGIATNFANREAVEQADVIVLAVKPQMMAQVCAEFADVDFSNKWVISVAAGISV
                     QRLETLLPSAKNIIRTMPNTPSLISEGMTGLFAKKSVNPTACQFAEQLMSAVGKCYWV
                     ETEEQINHIIAITGSSPAYFFRFMEAMQQSAVQMGFSEQDARTLVQSVALGAAKLVEA
                     NPTTPLSTLRENVTSKGGTTAKALEVFEQHDLAKTVDDAMWAAIHRAREMEQSL"
     gene            2325..3233
                     /gene="rdgC"
                     /locus_tag="PXD66_000456"
     CDS             2325..3233
                     /gene="rdgC"
                     /locus_tag="PXD66_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HDL6318790.1"
                     /translation="MVWFKNVMIYKLTGELALESNSLNELQQETRFTPCSQHDMSKFG
                     WATPLTGSDLLHFSQANQFLLISHKEDKLLPANVVKAETEQRIAELEEKEARKLKKTE
                     KQAVKDQVVSALLPRAFSKHQFTALWLDLDNQLVYVDAASSKRAEDTLALLRKTLGSL
                     PVVPISFTLPPAEVMTNWIAKGHTPSWLNLLEEAELKSFDTDSIIRCKRQDLETEEIA
                     RHLQAGKFITKLAIEWEGHFNCVLNEDATLSRLKFADEVREKNDDILKEDVAQRFDAD
                     FLLMTEELKLFTEKMIAEFGGIKSRI"
     gene            3254..3859
                     /locus_tag="PXD66_000457"
     CDS             3254..3859
                     /locus_tag="PXD66_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HDL6318791.1"
                     /translation="MLDQTIIDQYDALIFDMDGTLIDTMPSHAKAWEKVGEVLGYPIN
                     PKPMYELSGSTTFVIAREIMQRSNIPEHYFEQVVQLKREFGIEMVLANATLLPAFEII
                     KANVGKKPMAIGTGSHRAMVELLDQKFNLRQYVSVIVDSDDVSNHKPAPETFLKCAEK
                     LGIAPHRCLVFEDADLGVQAALNGNMNVFDVRTNTITHSTR"
     gene            3875..4666
                     /locus_tag="PXD66_000458"
     CDS             3875..4666
                     /locus_tag="PXD66_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDL6318792.1"
                     /translation="MKTEWIKHLIQQLTTLSKAIENYFTSGSFGVKFYPFLMHPCFRK
                     SCAFLIFIIAMVSSFSGLFYLVAEHITILFSERSLTTYFHHSTLELMIPVGTMIDEKI
                     SELSPLSVVMRSMFVLQAVVFFFIYAIGITHIAHNKLRVIGLLLSLCFAIGCSLVAGI
                     QPSSRGEFGIYNLGASITFLIGNLTLIIAGFDLYHPSMQFFKRFSIGAGVIGAICILI
                     TIFLPTLFSPLIERAGIYTIMLWEILAGFAIIKRLLKLPSFRQQG"
     gene            4670..5812
                     /gene="argE"
                     /locus_tag="PXD66_000459"
     CDS             4670..5812
                     /gene="argE"
                     /locus_tag="PXD66_000459"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016533842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HDL6318793.1"
                     /translation="MKQIPFIQRYAQLIELPTISSLVAEEDLSNRRLIELLATWLADF
                     GFKTEILAVEGSRNKYNLLATYGEGEGGLLLAGHTDTVPFDEGKWRFNPFQLTEKAGK
                     LYGLGTADMKGFFAFVVEVVSQLDLTQIKKPIRILATADEETTMLGARTFAQHSHIRP
                     DCAIIGEPTSLKPIRAHKGHIGESVRITGRSGHSSDPAKGINAIELMHQSIGYLMKMR
                     DELREKYHNPLFQVTHPTMNFGNIHGGDAINRICACCELQFDMRPLPNMAVQDLYAMV
                     NEHLKPMLEQYGDLIEIRHLHDGIPGYECEHSAQIVQVVEKLLGEKCDAVNYCTEAPF
                     IQQLCPTLVLGPGSIEQAHQPDEFLEMKYIEPTKELLTKLIYHFCG"
     gene            complement(5959..6246)
                     /locus_tag="PXD66_000460"
     CDS             complement(5959..6246)
                     /locus_tag="PXD66_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL6318794.1"
                     /translation="MPKSWELSLTARSNVIEILQSVEDYTGHWLSAVSLWEQLQNKLD
                     LIAFMPTIGRRREDGTLETFCRNYRIVYEIHLEKVYIITIIHSRRLYPRPS"
     gene            complement(6239..6430)
                     /locus_tag="PXD66_000461"
     CDS             complement(6239..6430)
                     /locus_tag="PXD66_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318795.1"
                     /translation="MLFKEQGYDEFLAEQIKAGQEAIARGETFTKEQINNELNHLFAD
                     MQKRQQLRENENLFEVNYA"
     gene            complement(6720..7310)
                     /gene="yraP"
                     /locus_tag="PXD66_000462"
     CDS             complement(6720..7310)
                     /gene="yraP"
                     /locus_tag="PXD66_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HDL6318796.1"
                     /translation="MNKLANKLLKGSLLVLGFISLQGCITTAVVTSAAVATKVATDPR
                     TTGTQVDDEILEEKVAYNINKDEQIKQEARINVVAYNGKVLLIGQAPSMDVVENAKNL
                     AAGAEGVTEIYNEIRQGEKIGFGQITQDSWITTQVKSKLLVNSEVKATEVKVVTENGE
                     VFLMGKVSQNQADAAAEAARNVGGVTKVVKVFSYAQ"
     gene            complement(7320..7904)
                     /locus_tag="PXD66_000463"
     CDS             complement(7320..7904)
                     /locus_tag="PXD66_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL6318797.1"
                     /translation="MLNKIQDRFTESIQTQISASELLPAKLSEAANRIVACLLRGDKV
                     IVCGHGRSYGNAELLVSHLLHRYELARPSFAAVQLQLSGVLAGVVAQDNELEAVYKKQ
                     LQAVAKEGDLFIVFSPVGNEECILQAIHTAKNQHLDLDIIALTGSNNDHTLGLLDESD
                     IEISAPSTNQLRVIESHHFYVNLLCELIDHLLFS"
     gene            complement(7910..8281)
                     /locus_tag="PXD66_000464"
     CDS             complement(7910..8281)
                     /locus_tag="PXD66_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HDL6318798.1"
                     /translation="MELFNKLINKREQGSFYEQKARVFLEQQGLRFVAANQAFKGGEL
                     DPIMQDGQTVVFVEVRQRKSNRFGSAVESIDYRKQQKWQNAANMWLLKQNQSLETANC
                     RFDVVAFEGNQTPVWIPNFLG"
     gene            complement(8256..9977)
                     /locus_tag="PXD66_000465"
     CDS             complement(8256..9977)
                     /locus_tag="PXD66_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HDL6318799.1"
                     /translation="MATILNHTMKKALVPTAIALFISACTSVNPVTESIKNEAYSSSE
                     FYINKADQTKEAEDKISYQLLAVRKLIDENKEYEAQNTFSEILTAEMNEVQKLEYALV
                     SAQLAALQGKNEQATSQLKAIQDPLLSSAQRLRYIQTQARIAANQKDVIGIVRARSQL
                     NNFYKMNRERQENNDIIWQTLRDANRGMLEKTVPEAGEMELAGWLALINIYNQNVTTP
                     AQMPQAINNWKAQYPSHSAVAVMPTELQGVSNFQQTQLNSVALLLPLSGDAKILGDII
                     KRGFNDAKADDSTAVQTFDTDSSDVNSLISQAKQQGANVIVGPLLKSRVDEMLLSPEI
                     QNVNILALNATENVRNIAQVCYYGLSPESEAQSGAEKIYQDGNSVAIVAAPQDDYGNR
                     SAEAFAKRWRQLTNSDADVRYYNQPLDAVAAIQNAGVSKAALYILGNAEQVLEIKQGI
                     DSSTLKDRLAIYTSSRSNSPNNGIDFYTSMEGVKFSEVPLLADQSSSEYKKAENLANS
                     DFSMMRLYAMGADAWSLATKFNEFRHIPGYKISGLTGELKAGQHCNIERSLSWMQYRN
                     GAIQQAY"
     gene            10051..10923
                     /gene="rsmI"
                     /locus_tag="PXD66_000466"
     CDS             10051..10923
                     /gene="rsmI"
                     /locus_tag="PXD66_000466"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDL6318800.1"
                     /translation="MKNRQNSSGINPNYGILYIVATPIGNLGDITQRALDTLSSVDLV
                     AAEDTRHSGLLLSHYGIKKPFFALHDHNEQQKAVVLVEKLAKGENIALISDAGTPLIS
                     DPGFHLVRHCRQAGVKVVPIPGACAAVTALCASGIASDRFCFEGFLPAKTKSRCDKLT
                     ELENEPRTLIFYESTHRILDTLADMQTVFGEDRYVVMAREITKTWETIYGDQLGNLIE
                     WLNEDSNRIKGEIVLVVEGKPQSSEEAFSSQAIKLLTLLCQELPLKKAAAIVAETFGY
                     KKNALYQYGLEHFE"
     gene            complement(11168..12334)
                     /locus_tag="PXD66_000467"
     CDS             complement(11168..12334)
                     /locus_tag="PXD66_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL6318801.1"
                     /translation="MTNLLLAIIYLSFISLGLPDAILGSAWPSMHQQLAVPLSYAGVV
                     SMIMSIGTITSSLMSDKLTYHLGTGKVMVISVALTALALWGFSISDAFWILCLWAIPY
                     GLGAGAVDAALNNYVAVHYASRHMSWLHCMWGVGASIGPHILGYALLQGRGWEAGYFT
                     ISMLQIGLTLMLFCSLPFWKLRKQITAEINHSAKPMSLKETLKIPGVKYGILAFFGYC
                     GVEQTAGLWASSYFVMVKDVGLEQAAAFGSIFFIGITVGRGVSGFLTLKFSNRQMIKL
                     GSALILVGIAILCLPLGKYVTLASLIIIGLGCAPIFPCLIHSTPAFFGEDKSQSVIGV
                     QMASAYIGVLLLPPIFGFIANAISIQLLPLYLLLMFAIMAYAYRNLVRETSPSP"
     gene            complement(12486..14000)
                     /gene="putP"
                     /locus_tag="PXD66_000468"
     CDS             complement(12486..14000)
                     /gene="putP"
                     /locus_tag="PXD66_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HDL6318802.1"
                     /translation="MFGLDPTTIMFVVYILGMIAIGFIAYRVTNNLSDYILGGRRLGS
                     FVTALSAGASDMSGWLLMGLPGAVYAGGLIEGWIAIGLTIGAYLNWKLVAGRLRSHTE
                     HSGDALTLPEYFHNRFGDKTRLIKILSAVIFLLFFAIYCASGVVAGARVFENLFGLPY
                     HQAIWYGAVATILYTFIGGFLAVSWTDTIQASLMVFALILTPFFVMYNLGGFDEMQAL
                     IDRAGEMAQKDYNDIFTGTSVLGIISLMAWGLGYFGQPHIVVRFMAAESVKSLENARR
                     ISMTWMIICLLGAIGIGYFGMAYFFGNPEQVGNISKNPEQIFIELAKLLFNPWVAGIL
                     LSAILAAVMSTLSAQLLICSSAITEDLYKGILRPQASDKELVWLSRLMVLAVAALAIY
                     IAQDPDSKVFGLVRNAWAGFGSAFGPVVLLSLFWKRMNGFGAIAGMLTGALIVHFWGD
                     ITLKLALPEIYSMIPGFIAATLMIIVVSLITPMPSKAVLDTFDRANKAYLAEVK"
     gene            complement(14094..14882)
                     /locus_tag="PXD66_000469"
     CDS             complement(14094..14882)
                     /locus_tag="PXD66_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDL6318803.1"
                     /translation="MNAKKEKESSGNQSLIRGLTLLELLAKFPNGCPLAHLAELSGLN
                     KSTVHRLLQGLQQEGYVRPAPTAGSYRLTTKCLAIGQRALSSINILHIAAPHLEALNL
                     TLGETVNFAMRENDHAILINKLEATTGMMRTRAYIGQQMQLYCSGMGKLFLAYGSADY
                     IPQYWQTKSDIIQKLTVNTITTIDGMQTELETIRRQGFAMDNEENELGVSCIACPIFD
                     HQQKVHYAISVSLSTARLNQLGRENLLPAIQETAEKISAELGGR"
     gene            complement(14879..15457)
                     /locus_tag="PXD66_000470"
     CDS             complement(14879..15457)
                     /locus_tag="PXD66_000470"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDL6318804.1"
                     /translation="MNQSSLTDLRSQLRQSMRLKRQSLTAGQQTQAAESIIPQALSLI
                     ESYQASHIAFYLPFNGEISPIPLMEQLLQQGKKLYLPLLHPFTSGQLLFVNYNCKTIL
                     KFNRLGIQEPILDVRNILPLQELEMIFTPLVACDKAGNRLGMGGGFYDRTLAQAPHLI
                     SVGLAHECQQVEQLPIESWDMPLDHIILGAAK"
     gene            complement(15459..15650)
                     /gene="ssrS"
                     /locus_tag="PXD66_000471"
     ncRNA           complement(15459..15650)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PXD66_000471"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(15755..16045)
                     /locus_tag="PXD66_000472"
     CDS             complement(15755..16045)
                     /locus_tag="PXD66_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDL6318805.1"
                     /translation="MSANNIELQIFGQVLRLQCPVEQQEHLLASAQRLEERVVVLKEQ
                     SGIIQLEKVLAIVALNLNYELEQAQRQNAENKNVLVACIEQLDNSLGKFQSN"
     gene            16124..16681
                     /locus_tag="PXD66_000473"
     CDS             16124..16681
                     /locus_tag="PXD66_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HDL6318806.1"
                     /translation="MAITDSQQFKQLVSQLQIDDTPAEFHGFLCGLIAGGIQDESWKT
                     LTYQFTNDGHAFSIEPLNTLTDFYQQLSESFSEANTLFSLWLPQNEEDGFALADGISE
                     WTNSFLLGLGVAQPKLQQETDEVGEAIDDLDEIAKLGYSDDDNNEELLEAGEEVLEYL
                     RVLALFLHSHFALGVPKTEEKPPLH"
     gene            16779..17843
                     /gene="hemE"
                     /locus_tag="PXD66_000474"
     CDS             16779..17843
                     /gene="hemE"
                     /locus_tag="PXD66_000474"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HDL6318807.1"
                     /translation="MSELKNDRYLKALLREPVDMTPVWMMRQAGRYLPEYKATRAEAG
                     DFMSLCRNADLACEVTLQPLRRYELDAAILFSDILTVPDAMGLGLSFGAGEGPKFARP
                     VASLNDVNQLPIPDPEDELQYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTKIKKMLYAEPHILHKLLDKLADSVILYLNAQIKAGAQAVMVFDTWGGVLAHED
                     YKAFSLHYMHKIVDGLIRENEGRKVPVTLFTKGGGLWLEAIAETGCDAVGLDWTVDIA
                     DARRRIGDKVALQGNMDPSVLYASPERIEQEVKNILAGFGNGSGHVFNLGHGIHLDVP
                     VESPKVFVDAIHNYSKQYHK"
     gene            17990..18580
                     /locus_tag="PXD66_000475"
     CDS             17990..18580
                     /locus_tag="PXD66_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF416 family protein"
                     /protein_id="HDL6318808.1"
                     /translation="MRNPIHKRMERFEPWQNTTFMACLCERMYPNYQLFCEVTEQPEK
                     AKVFQNILNLVWEYLTIKGAKINFDNQLEKLEEIIPDVNEYAFFGVLPAQEACEALSE
                     LLHSIIAGSTLEQAIRISQISLGTVASYLEMQQDKELNEQELKNSEEIQEELDVQWQI
                     YRLLNECEKRDLDLIFELKDEIRSSGISNIGLNINQ"
     gene            18716..>18945
                     /locus_tag="PXD66_000476"
     CDS             18716..>18945
                     /locus_tag="PXD66_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005819506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDL6318809.1"
                     /translation="MNKTELIDAIAAGANLTKKDAKAALEATLDAISASLKAGDAVQL
                     IGFGTFKVNERKARTGRNPKTGEEIKIAAAKVP"
BASE COUNT         5609 a         4221 c         3898 g         5217 t
ORIGIN      
        1 aattttatgt cagcaattac cggcacagta aaatggttca acgccaccaa aggttttggt
       61 tttatcaccc ctgatcaagg tggcaaagat gtattcgtac acttctctgc tatcgaagca
      121 aacggcggct accgcacatt agaagacggt gctaaagtac aatttgaaat ccaagattca
      181 gagcgtggag cttcagccgt aaacgtgaaa acaatctaat ttcagcaaat tcaatgaatt
      241 tcgataaaaa tgccgacaac ttgtcggcat ttttgctatc aattaatctg tacttcaaac
      301 cttttcggca caataaaaat ggcaggtagg gtaaaaattg ccatcaacca aaatgtcagg
      361 cttggggaat gttgatagac cattccggca ataaaggtaa atactgccat aaaagcacag
      421 cttgccaagc caaaatagag accttgtaat ttcgtcacac gctctgccga ttgcatcgaa
      481 atatagcgaa tcatcgcaat atgagccgcc ccgaaggtaa tagaatgtag catttgcatt
      541 acaataatca gcacgatctc tgtagtacta cccattattg cccaacgaat cactgcaaaa
      601 acggcagcta aaatcattaa atggctgatt ttccacgatt taaacaagcg tttagagacc
      661 aaaaagaaca aaatttccgc caccaccgcc aagccccaaa acaggctagt cattgcggtt
      721 gaaatactgg catttgacca atgaatggtg ctgtaagtgt aataagcggc gtgcgaggct
      781 gaaattaacg acaccgcgat caacattttt aaggtttccg gctctttcaa taattggcga
      841 taagttacgc ttgaagtatt ggctttatta ccttgttctt caaacccgac tttgggcgta
      901 gcaagctgcc ctattcctaa aaaaaccaag aaaccgatga taatccacac caccgaattt
      961 tcaccaagca agccgataag atagccggca gaaagtgagc ctaccacaaa cgccattgag
     1021 ccgaataacc ttgctctgcc gtaatccagc ccgatctgat gttgccaaat tgaggcaatg
     1081 ctatcggcta tcggcattgc ccccgaattg aacatttgaa acaacattaa caccggaaaa
     1141 agtagccaaa tgaaatcccc tgaaaacagc aacgccgccg cagccgcaat attcagccaa
     1201 gttaaactgc gggcggtggg aatgagttgg ctcacggttt ttacccgttg tgaggcaagc
     1261 atactgccgg caaagcggaa caaatagcca acggcggcaa ataagccgat catttcagtc
     1321 gaatagccgt gatgtttaag ccacactggc aaaaacggca acaacacgcc ataggcacaa
     1381 aaaaagccaa agtaattaaa tgccgcccat tggaacgggg aaactttcat cataacgatt
     1441 gctccatttc tctggctcga tgaattgccg cccacattgc atcatcaacg gttttagcaa
     1501 gatcgtgctg ctcaaacacc tcaagggctt tcgccgttgt gcctcccttt gaggtcacat
     1561 tttcccgcaa ggtcgaaagt ggcgtagtcg ggttcgcttc caccaatttc gccgccccca
     1621 atgcgacaga ttgtacaagc gttcttgcgt cctgctcgga aaagcccatt tgcaccgcac
     1681 tttgctgcat tgcctccata aaacggaaga aatacgccgg gcttgagccg gttatcgcaa
     1741 taatgtggtt gatttgctcc tcggtttcca cccagtaaca tttaccaacg gctgacatca
     1801 actgttcggc aaattggcaa gcggtcggat ttaccgattt ttttgcaaat aatccggtca
     1861 ttccttcgct gattaaagac ggggtgtttg gcatcgtgcg aatgatattt ttcgcactcg
     1921 gcaacagcgt ttccaagcgt tgcacagaaa tacctgctgc tactgaaatc acccatttgt
     1981 tggaaaaatc aacatcggca aactcggcac acacttgtgc catcatctgt ggttttaccg
     2041 ccagcacgat cacgtcagcc tgctctaccg cctcacgatt tgcaaaattt gtcgcaattc
     2101 cgaccgcttg taactcctct cggcgagcca aattactttt attgcacgca atgatttgtt
     2161 cggcagaata accgcttttt aataagcctt gaataatcgc atacgccata tttccggtgc
     2221 cgataaaggc aagttttttc tcgttcattc gcaaattctc tactgtgttg ggttgaggtc
     2281 tgttaaaata acactattct acacccgata aaggatatgc aaaaatggtt tggttcaaaa
     2341 atgtgatgat ctataaatta accggcgagc tggcgttgga aagcaatagc cttaacgagt
     2401 tacagcaaga aacccgcttt accccttgca gccagcacga tatgagcaaa ttcggctggg
     2461 ctacgccact taccggctcg gatttactgc atttctcaca agcgaaccaa tttttgctga
     2521 tttcacacaa agaagataaa ttattacctg ccaatgtggt gaaagccgaa accgagcaac
     2581 gcattgcaga actggaagaa aaagaggctc gtaagctgaa aaaaaccgaa aaacaagccg
     2641 tgaaagatca ggtcgtttct gcgttattgc ctcgagcatt cagcaaacat caattcaccg
     2701 ctctttggtt ggatttagat aatcagttgg tttatgtgga tgcagcctcg agcaaacggg
     2761 cggaagatac gctggcgtta ctgcgaaaaa cacttggctc gttgccggtt gtgccgattt
     2821 cctttaccct accgccggca gaggtaatga cgaattggat cgccaaaggg catacgccaa
     2881 gttggttgaa tttgttggaa gaggcagaac tgaaatcttt cgataccgac agcattattc
     2941 gctgtaaacg ccaagattta gagacggaag aaatcgcccg gcacttgcaa gcgggcaaat
     3001 ttatcacaaa acttgcgatt gaatgggaag ggcatttcaa ttgcgtgtta aatgaagacg
     3061 ccacgctttc acgcctgaag tttgccgatg aagtgcgaga gaaaaatgac gatattctca
     3121 aagaagatgt ggctcaacgc tttgatgccg atttcttatt aatgaccgaa gagctgaaac
     3181 ttttcaccga aaaaatgatt gccgaatttg gcggaattaa atccagaatt tagaaaaaaa
     3241 tttaaggaaa gcaatgttag atcaaaccat tatcgaccaa tatgacgcct tgatttttga
     3301 tatggacggc acattaattg acacgatgcc aagccacgcc aaggcgtggg aaaaggtggg
     3361 cgaagtgtta ggctacccaa tcaatccaaa gccaatgtac gaactgagtg gctcaaccac
     3421 ttttgtgatt gcccgagaaa taatgcaacg ctccaatatc cccgagcatt attttgagca
     3481 agtggtacaa ctaaaacggg aatttgggat tgaaatggtg ttagccaatg ccactctgtt
     3541 gcctgccttt gagattatta aagctaacgt tggcaaaaaa ccaatggcaa tcggcacagg
     3601 ctcacaccgt gcaatggtgg aattattaga ccaaaaattt aacttacgtc aatatgtttc
     3661 ggttattgta gattctgatg atgtaagcaa ccataagcca gcccctgaaa cctttttaaa
     3721 atgtgcggaa aaacttggca ttgccccaca ccgctgtttg gtgtttgaag atgccgattt
     3781 aggcgtgcaa gccgccctca acggcaatat gaacgtgttt gatgtacgta cgaatacgat
     3841 aactcactca acccgttaaa aatgataggt ttatatgaaa acagaatgga ttaaacattt
     3901 aattcaacaa ttaaccacgc tttccaaagc catcgaaaac tactttacca gcggttcatt
     3961 tggggtgaaa ttctaccctt ttctaatgca cccttgtttt cgtaaaagct gtgccttttt
     4021 gatttttatt attgctatgg tttcaagttt tagcgggcta ttttatttgg tggcggaaca
     4081 catcacgatt ttatttagcg aaagatcgct caccacttac ttccaccata gcacccttga
     4141 attgatgatt ccagtcggca caatgataga tgaaaaaatc agcgaattat caccactttc
     4201 ggtggtaatg cgttcaatgt ttgtgctaca agcagtcgtt tttttcttta tttatgcaat
     4261 cggcatcacc cacattgctc acaacaaatt gcgggtcatc ggcttattgc tttcgctatg
     4321 ctttgcgatt ggctgctcac tagttgccgg tattcagcca agcagtcgtg gcgaattcgg
     4381 catttacaat ttaggggcaa gcattacgtt tttaatcggc aatttaacgc tgatcatcgc
     4441 aggcttcgat ctctaccacc cttcaatgca attttttaaa cgctttagca ttggtgcggg
     4501 cgttatcggg gcaatctgta ttttaatcac gattttccta ccaaccctgt ttagcccgtt
     4561 aattgaacga gcaggtattt ataccattat gctgtgggaa attttagccg gttttgccat
     4621 cattaaacgc ctgttgaaat tgccctcatt cagacaacaa ggataaccaa tgaaacagat
     4681 tccctttatt caacgctatg ctcaactgat tgagctaccg accatttcca gccttgttgc
     4741 cgaagaagat ttatcgaacc gccgtttgat tgaattgctc gccacttggc tggcagattt
     4801 cggctttaaa accgagattt tagcggtaga aggcagccgt aataaataca atttacttgc
     4861 gacctatggc gagggtgagg gcggcttatt attggcaggg cataccgaca ccgtgccgtt
     4921 tgatgaagga aaatggcgct tcaatccatt ccaattaacc gaaaaagcag gcaaactcta
     4981 cggcttaggc acggcagata tgaaaggctt tttcgccttt gtggtggagg ttgtgagcca
     5041 actcgaccta acccaaatca aaaagccgat tcgcattctt gccaccgctg atgaagaaac
     5101 cacaatgctg ggggcaagaa cctttgctca gcacagccac attcgccccg attgtgcgat
     5161 tattggcgag ccgacctcgc tcaaaccgat tcgagcccac aaagggcata ttggcgaatc
     5221 ggttcgcatt accggcagaa gcggacactc cagcgacccc gccaaaggca ttaatgcgat
     5281 tgaattaatg caccaatcta tcggttattt aatgaaaatg cgtgatgaac tgcgagagaa
     5341 ataccacaat ccgctgttcc aagtcaccca cccgacaatg aacttcggca atattcacgg
     5401 cggcgatgcc attaaccgca tttgtgcctg ttgcgaattg caatttgata tgcgaccact
     5461 accgaatatg gcggtgcaag atttgtatgc aatggtaaac gagcatttaa aaccgatgct
     5521 ggaacaatat ggcgatttaa ttgaaatccg ccatttacac gatggaattc cgggctacga
     5581 atgcgaacac tcggcacaaa tcgtgcaagt ggttgaaaag ctactgggcg aaaaatgcga
     5641 tgccgtcaat tactgcaccg aagccccatt cattcaacaa ctctgcccaa cgctggtact
     5701 aggccccggc tccattgaac aagcccacca gcccgatgag tttttggaaa tgaaatatat
     5761 cgaaccgaca aaagaattat tgactaaatt gatttatcat ttttgtggat aaatcaacag
     5821 aaacaagcgc tcattttcgc catacttttt gcaaaagaat cggcaaaaat gaccgcttga
     5881 tatttagtat tgagtgacct ataaacttcc atacttcatc attgatttaa ggagattctg
     5941 ttagataggt attaatctct agctaggtct tggatacaaa cggcgagaat gaattatagt
     6001 aataatatat actttttcta ggtgtatctc atagacaata cgataattgc ggcagaatgt
     6061 ttctaatgtg ccatcttctc tacgtcgccc tattgtaggc ataaatgcaa tgagatcgag
     6121 tttattttgt aattgttccc aaagtgaaac tgcgctcaac caatgccctg tataatcttc
     6181 taccgactgt aaaatctcaa tgacattaga ccttgcggta agtgacagct cccaagattt
     6241 aggcataatt aacctcaaac aaattttcat tttctcgtaa ttgctgacgt ttctgcatat
     6301 ccgcaaataa atgatttaat tcgttattga tttgttcttt ggtaaaggtt tctcctcgag
     6361 ctattgcttc ttgaccagct ttaatttgct ctgcaagaaa ctcatcatat ccttgttctt
     6421 tgaatagcat attctgctcc tttgatttgt gtcttgattc cactatgttc aagacatagt
     6481 ttaagtttaa atctacgctc aaagcaatga agagtcaagg gaatccttat gttgataggg
     6541 tgtgcatatt tttcgagtca aatgaattaa aaatcatcat tacgatcacc cactcataga
     6601 ttttatcttc ctatctaagg tcatcttctt atatcaaaca agcggtctaa atttgcaaaa
     6661 aaatgacaaa tttagaccgc ttgtgagtta caaaaatata aactaattga gcttatcaat
     6721 tactgcgcat aactaaatac tttcaccact ttggttactc cacctacatt gcgagcggct
     6781 tcggcggcgg catcagcttg gttttggctg actttgccca ttaaaaacac ttcgccgttt
     6841 tcggtgacga ctttcacttc ggtggctttt acctcgctat ttactaacag tttggatttc
     6901 acttgggtgg taatccagct gtcttgtgta atctgtccaa agccgatttt ttcgccttgg
     6961 cggatttcgt tgtaaatttc cgtcacgccc tccgccccag cagcgaggtt tttcgcattt
     7021 tccactacgt ccatacttgg tgcttgcccg attaataaca ctttgccgtt gtaagccacc
     7081 acgttaatgc gggcttcttg tttgatttgc tcgtctttat taatgttgta agcgactttt
     7141 tcttctaaaa tttcatcatc aacttgcgtg ccggtggtgc gtgggtctgt cgccacttta
     7201 gttgcaaccg ccgcagaagt caccaccgcc gtggtgatac aaccttgtaa agagataaaa
     7261 cctaatacta ataggctgcc ctttaatagt ttatttgcta atttgttcat aatctctcct
     7321 tacgaaaata gtaaatggtc gataagttca cataataaat tcacataaaa atgatggctt
     7381 tcaatcaccc tcagttgatt ggttgaaggg gctgaaattt caatatcact ttcatctaat
     7441 aagccgagtg tgtggtcgtt attactgcct gttaaagcaa taatatctaa atctaaatgt
     7501 tgatttttcg ccgtatgaat agcttgcaat atacattctt cattacccac aggcgaaaaa
     7561 acaatgaaga gatcaccctc ttttgcaacc gcctgcaact gttttttata aacagcctct
     7621 aactcgttat cttgtgccac aactcctgcc aatacaccgc ttaattgcag ttgtacggca
     7681 gcaaaactcg gtcttgccag ttcataacgg tgaagtaagt ggctgaccaa taattcggca
     7741 ttaccgtaag agcgaccgtg tccgcagacg ataactttat cgccacgcaa caaacaggca
     7801 acaatgcggt ttgccgcttc acttaattta gccggcaata gctcagaggc ggaaatttgg
     7861 gtttgaatac tttcggtaaa tctatcttgg attttattta acattctttc taacctaaaa
     7921 agttgggaat ccacacaggc gtttggtttc cttcaaaagc aaccacatca aaacggcaat
     7981 ttgcagtttc taaactttgg ttttgtttaa gcaaccacat attagcggca ttttgccatt
     8041 tttgttgctt gcggtaatca atactctcaa cggctgaacc gaagcggttg cttttacgct
     8101 ggcgaacttc tacaaacaca acggtttgtc cgtcttgcat aatcggatcc agttcgccac
     8161 ctttaaatgc ttggttagcg gcaacaaaac gaagcccttg ctgttctaaa aacactcggg
     8221 ctttttgttc ataaaacgag ccttgttccc gtttattaat aagcttgttg aatagctcca
     8281 ttgcgatatt gcatccaaga taaactacgt tcaatattac aatgttgtcc ggcttttaac
     8341 tcaccggtta aacctgaaat tttgtagccc ggaatatggc ggaattcatt gaatttggtt
     8401 gccaatgacc acgcatcagc ccccattgcg tataaacgca tcattgagaa atcgctattg
     8461 gctaaattct cggctttttt atattcacta gaactttgat cagctaacag tggcacttca
     8521 ctaaatttta ccccttccat tgaagtgtaa aaatcaatgc cgttatttgg cgaattactg
     8581 cgagatgagg tatagatcgc taaacgatct tttaaggttg agctatcaat accttgttta
     8641 atttctaata cttgctcggc attccccaaa atatataaag ctgctttgct cacacctgca
     8701 ttttgaatag cggcaacggc atcaagcggt tggttgtagt aacgcacatc agcatcgcta
     8761 ttggtaagct gacgccagcg tttggcaaag gcttctgccg agcggttgcc gtagtcatct
     8821 tgaggggccg caacgatagc gacactatta ccgtcttgat aaattttttc cgcccctgat
     8881 tgtgcctcag attcaggaga taaaccgtag taacacacct gagcaatatt acgcacattt
     8941 tcagtggcat tcagtgccaa aatattcacg ttttggattt ctgggctaag tagcatttca
     9001 tcaacacgag atttgagcaa tggcccgaca atcacatttg ccccttgctg ttttgcctgt
     9061 gaaattaagg aattaacatc agatgaatcg gtatcaaaag tttgcactgc ggttgagtca
     9121 tcagccttag catcattaaa accacgcttg ataatatcac ccaaaatttt tgcatcacca
     9181 cttagaggca atagcaatgc gacactgttt aactgggttt gttggaaatt actcacccct
     9241 tgtaattccg ttggcattac ggcaaccgca ctatggctcg ggtattgggc tttccaatta
     9301 ttaattgctt gtggcatttg tgccggtgtc gtcacatttt gattatagat attaattaat
     9361 gccaaccaac cagcaagctc catctcaccc gcttcaggca cagttttttc cagcattcct
     9421 cgatttgcat cacgcaaggt ttgccaaata atatcgttat tttcttggcg ttcacggttc
     9481 attttataga aattgttaag ttgcgatctt gcacgcacaa tgccaatcac atctttctga
     9541 ttggcagcaa tacgggcttg ggtttgaata taacgcaaac gctgtgccga acttaatagc
     9601 ggatcttgaa tggcttttaa ttggcttgtt gcttgctcgt ttttcccttg taaggcggca
     9661 agctgggcgg aaaccaaggc atactctaac ttttgcacct cgttcatctc tgcggtcaga
     9721 atttcactaa aggtattttg agcctcatat tccttatttt catcaattaa tttgcgtaca
     9781 gctaaaagtt gatacgaaat tttatcttcc gcttctttgg tttgatctgc tttattaata
     9841 tagaattccg agcttgaata ggcttcattt ttaatggatt cggtgacagg atttaccgat
     9901 gtacaggcag aaatgaataa tgcaatcgcc gttggcacca atgccttttt cattgtatgg
     9961 tttaaaatag ttgccattgt cagatcctca atagaatata atgtgctaaa tttaatgtga
    10021 aatcttactt atcaaacgag ctaaaagcaa atgaaaaaca gacaaaattc atctggaatc
    10081 aacccaaatt acggaatttt atatattgtt gccacgccta tcggcaattt aggcgatatt
    10141 acccaacggg cgttagacac cctttcatct gtcgatttag tcgccgccga agacactcgc
    10201 cacagcggtt tattgctgag ccactacggc attaaaaaac cgtttttcgc cttgcacgac
    10261 cataatgaac agcagaaagc ggtcgtttta gtcgaaaaat tagcaaaagg ggaaaacatt
    10321 gcgttaattt ccgatgccgg aacgccactg attagcgacc cgggctttca tcttgtccgt
    10381 cattgccgcc aagccggggt aaaagttgtg ccgatccccg gggcttgtgc cgctgttact
    10441 gccttatgtg cttcaggcat agcttcagac cgtttttgtt ttgaagggtt cttaccggca
    10501 aaaacaaaat cccgttgcga taaacttacc gagcttgaaa acgagcctcg cacactgatt
    10561 ttttatgaat ccacccaccg tattttagac actcttgccg atatgcagac tgtttttggc
    10621 gaagatcgct atgtggtaat ggcgcgtgaa atcacgaaaa cgtgggaaac catttatgga
    10681 gatcaactgg gtaacttaat tgaatggcta aatgaggata gcaaccgaat taaaggcgaa
    10741 attgttctgg tggttgaagg taaaccgcaa agtagtgagg aagccttttc cagccaagcg
    10801 atcaaattac tgacattact ctgccaagaa cttccgctta aaaaagccgc tgccattgtg
    10861 gcagaaacct tcggctataa gaaaaatgcc ctgtatcaat atggattgga gcattttgag
    10921 taacagacaa gcggtcagat tttgtgagtt ttttgcagat tggcactccc ctctctctga
    10981 aaaatgtttc tgaacgaaac atttcccggt ctcaatactc gctacgctcg ctccttcgga
    11041 gccgttggca aagccaacgt tcaaatgtaa acatttgtcc cataagggga gagagctaaa
    11101 ttattcgctt gtcgacccct ctccactcgt gggagaggga cagattcttc ttcgttcaga
    11161 agaaaaatca gggagagggg gaagtttctc gcactaaatt ccgataagca tatgccataa
    11221 tcgcaaacat caacagcaag taaagcggta ataattgaat gctgatagca ttagcaataa
    11281 agccgaaaat cggtggcaat aacaggacgc cgatataggc acttgccatt tgcacgccga
    11341 tcaccgattg agacttgtcc tcgccgaaaa acgcaggggt cgaatgaatt aagcacggga
    11401 aaatcggggc acaacctagc ccgataataa ttaaacttgc cagcgtaaca tatttgccta
    11461 acggcaagca cagtatcgca atgccgacta agatcaaggc tgaaccaagt ttaatcattt
    11521 gtctgttgct aaatttaagc gtcaagaagc cacttacgcc tctgccaacg gtaatgccga
    11581 taaagaaaat actgccgaac gcagccgctt gttctaagcc gacatcttta accattacaa
    11641 aatagctact cgcccacagt ccggcggttt gttctacgcc acaatagcca aaaaaagcta
    11701 aaatgccata tttcacgccc ggaattttta gcgtttcttt taagctcatc ggttttgccg
    11761 aatggttgat ttcagcggta atctgcttgc gtaatttcca aaaaggcagt gagcaaaaca
    11821 gcattagcgt tagcccgatt tgtaacattg aaatggtgaa atagcctgcc tcccagcctc
    11881 gcccttgcaa taaggcgtag cctaaaatgt gcggcccgat agaagctcca acgccccaca
    11941 tacaatgcag ccagctcata tggcggcttg cgtagtgaac tgctacatag ttattcaacg
    12001 ccgcatcaac cgctccagcc cctaagccgt aaggaattgc ccataaacat aagatccaga
    12061 aagcatcact gattgaaaag ccccacaatg ctaaagccgt taaggcaacg ctgatgacca
    12121 ttactttgcc tgtgccgaga tggtaggtca gtttgtccga cattaagcta gaggtaatag
    12181 tgccaatcga cataatcatc gagaccacac cggcatagga aagcggaaca gcaagctgtt
    12241 ggtgcattga cggccacgcc gagcctaaaa ttgcatcagg caacccaagg ctgataaaag
    12301 agagataaat aatagcgagt aggaggttag tcatagctta caatcggtca caaaaagcat
    12361 cgctttttga acgttggctc tgccaacggg agcgaagctg tgaacaatcc ttacaaggat
    12421 tgtgaataat ttttgataaa ttttttgtaa aaaaatggct taaggagacc ttaagccaaa
    12481 ttttattatt tcacttctgc caaataggcc ttattagcac ggtcaaaggt gtctaatacg
    12541 gctttgcttg gcattggcgt gataagtgat accacaatga tcattagggt agcagcaata
    12601 aagcccggga tcatcgagta aatttccggt aaggcgagct taagtgtaat atcgccccaa
    12661 aaatgcacga ttaacgcacc ggtcagcatt ccggcaatcg ccccaaaacc gttcatacgt
    12721 ttccagaata gtgaaagcaa taccacaggc ccaaatgccg agccgaaacc tgcccacgca
    12781 ttacgcacta agccaaatac tttgctgtcc gggtcttggg caatataaat agctaatgct
    12841 gccaccgcaa gcaccatcaa gcggcttaac caaaccagtt ctttatcgga ggcttgaggg
    12901 cgtaaaatgc ctttgtataa atcttcggta atcgcacttg aacaaatcag taattgagcg
    12961 gaaagggtac tcattaccgc agcaagaatg gcagataata aaatgccggc tacccaaggg
    13021 ttaaataaca atttagctaa ttcaatgaaa atttgttccg gatttttgct gatattgccg
    13081 acttgctccg ggttgccaaa gaaatatgcc ataccaaaat aaccaatgcc tattgccccc
    13141 agtaagcaga taatcatcca agtcatactg atacggcgtg cattttcaag agacttcaca
    13201 ctttctgccg ccataaaacg caccacaata tgcggttgtc cgaaataacc cagcccccac
    13261 gccattaagc tgataatgcc taatacgctg gtgccggtga aaatgtcgtt gtagtctttt
    13321 tgtgccatct cgcctgcacg gtcgattaag gcttgcattt catcaaaacc acctaagtta
    13381 tacatcacaa aaaacggggt gagaatcagg gcaaaaacca ttaatgaagc ttgaatagta
    13441 tccgtccaac ttactgctaa gaaaccacca ataaaggtgt aaagaatggt agcaacagcc
    13501 ccataccaaa ttgcttggtg gtaaggtaag ccaaataagt tttcaaatac cctcgcaccg
    13561 gccacaacgc ctgatgcaca gtaaattgca aaaaagagta aaaaaatgac cgctgataaa
    13621 atcttaatta aacgggtttt atcgccaaaa cggttatgaa aatattcggg taaggttaag
    13681 gcatcgccac tgtgttctgt atgcgagcgt aatctgcctg cgactaattt ccagtttaga
    13741 taagcaccga tagttaaacc gattgcgatc cagccttcga tcaaaccgcc tgcataaacg
    13801 gctccgggta atcccatcag caaccagccg gacatatctg atgcaccggc agaaagtgcg
    13861 gtcacaaagc tacctaaacg gcgaccgcct aaaatatagt ctgaaaggtt attggtaaca
    13921 cgataggcaa taaagccgat ggcaatcatt cctagaatat agacaacaaa cataattgtt
    13981 gttggatcaa gaccaaacat tattaatgaa tcctcaagat ttcttattgt ataaagatta
    14041 aagtgagtaa agggtaaaag attacctgaa ataatcaaaa tggcaacaca aaattaccgt
    14101 ccgcccagct cagctgaaat tttttctgct gtttcttgaa tagccggtag tagattttcc
    14161 cgccccagct gatttaaacg agcagttgaa agcgaaacag aaatcgcata atggactttt
    14221 tgttggtgat caaaaatcgg gcaagcaata caagaaaccc ctaattcatt ttcttcgtta
    14281 tccattgcaa aaccttgacg gcgaatggtt tccagctcgg tttgcattcc gtcaatcgtg
    14341 gtgatggtat tgaccgtcag tttttgaatg atgtccgatt tagtctgcca atattgtgga
    14401 atgtaatctg ccgaaccata agcaagaaac agttttccca ttcccgagca atagagttgc
    14461 atttgttgcc cgatataggc tcgggtacgc atcattccgg tggtagcttc taatttattg
    14521 attaaaatag cgtgatcgtt ttcccgcatc gcaaaattta ccgtctcgcc taaggttaaa
    14581 tttagggctt ctaaatgtgg ggcggcaata tgcaaaatgt taatcgaact taaggctcgc
    14641 tgcccgattg ccaaacattt ggtggttaaa cgataactgc ccgcagtcgg tgcagggcgg
    14701 acgtagccct cttgttgtaa gccttgtagc agacggtgaa ccgtgctttt gttcagcccc
    14761 gaaagctcgg ctaaatgggc aagcggacag ccgtttggga atttcgctaa caattccaat
    14821 agtgttaagc cacgaattaa actttgattt ccactcgatt ccttttcttt ctttgcattc
    14881 atttggctgc tcccaaaata atatgatcta acggcatatc ccaactctca atcggtaatt
    14941 gttcgacttg ctgacattcg tgtgccaaac cgacactaat cagatgaggt gcttgagcaa
    15001 gggttctatc ataaaagcca cctcccattc ccagccgatt tccggcttta tcacaagcaa
    15061 caagtggggt aaaaatcatc tctaattctt gcaacggaag gatatttcta acatctaata
    15121 tcggctcttg aatccccaag cggttaaatt ttagaatagt tttgcaattg taatttacaa
    15181 acaataattg accgcttgta aaagggtgca acagcggcag atacagcttt ttgccctgtt
    15241 gaagtaattg ttccattaaa ggaataggag aaatttcacc gttaaaaggc aaataaaagg
    15301 caatatgtga ggcttggtaa gattcaatca atgaaagagc ttgaggaata atggattcgg
    15361 ctgcttgagt ttgctgaccg gcagttaaac tttgccgttt aagccgcatt gattgccgta
    15421 attggctacg aagatcggtt aaagatgatt gattcatata aagacccgag atgccgctac
    15481 cagtaatggt ccttgaacca gtcggttcaa gggagtcaaa cttaactgct tttaggcttc
    15541 ttactcctaa ttaaattagg gtaagcttgc tcaccagcgt tagatgtcca actcaaagtt
    15601 tcaaattatc ggctcaggga cgtaactcgt aacgcacacc ccaggcaaga cttatagtaa
    15661 cggaatttga tgatttttcc tagtatttaa tcccacaaaa tgcaaattct caatcttttt
    15721 ggctctattc ctaatcaatt cgattaaaaa ttacctaatt ggattgaaat ttgccaagcg
    15781 aattatctaa ctgctcaata caagccacca acacattttt attttctgca ttttggcgtt
    15841 gggcttgctc cagttcataa tttaaattca gtgccacaat cgctaacact ttttcaagct
    15901 gaataatgcc ggactgctct ttcaatacca ccacacgctc ttccaaacgc tgtgctgaag
    15961 ccagtaaatg ctcttgctgt tcaacagggc attgcaagcg taaaacctgc ccaaaaatct
    16021 gtagttcgat attatttgcc gacataaata cgaaatcctc acttaatcgc aaaatttttg
    16081 ctatattatg ccattcttta gcaaaatcac aaataatccc attatggcta tcacagattc
    16141 ccaacaattt aaacaactgg tatcacaatt acaaattgat gatacaccgg cagagtttca
    16201 cggtttttta tgcggtttaa ttgccggcgg tattcaagat gagtcttgga aaaccctcac
    16261 ctaccaattt accaatgacg gacacgcatt ttccatcgag ccgttaaaca ctttaaccga
    16321 tttttatcag caattaagtg aaagcttttc agaagccaac acgctgttta gcctttggtt
    16381 gccacaaaac gaagaagatg gttttgcctt agctgatggt atctccgaat ggacaaacag
    16441 tttcttactt ggacttggcg ttgctcaacc taaattacaa caagaaaccg atgaggttgg
    16501 cgaggcaatc gatgatttag atgaaatcgc caaactaggc tacagtgacg atgataataa
    16561 cgaggaacta ctggaggctg gcgaagaagt gttggaatat ctgcgagttt tagccttatt
    16621 cttgcacagc cattttgctt taggcgtgcc gaaaaccgaa gaaaagccac cattacacta
    16681 atgaataaat acaagcggtt caatttcatc tctattttgc aaatatatta tgcggggcga
    16741 aaaatatttc gcccctacca aaatcaaagg aaaaaccaat gtcagaatta aaaaacgatc
    16801 gctatttaaa agcgttattg cgtgaacctg tggatatgac cccggtctgg atgatgcgtc
    16861 aagccgggcg ttatttgccg gaatataaag ccacccgagc agaagccggc gattttatgt
    16921 cactctgccg taacgctgat ttagcgtgtg aagtcacctt acaaccgcta cgccgttatg
    16981 aactcgatgc agctatccta ttttccgata ttttaactgt gcctgatgca atgggattag
    17041 gtttaagttt tggtgcaggt gaaggcccta aatttgcccg cccggtggca agcctcaatg
    17101 atgtcaatca gctaccgatt cctgatccgg aagacgagtt gcaatatgta atgaatgcgg
    17161 tgcgtaccat tcgccgagaa ttgaaaggcg aagtgccact aattggtttt tctggcagcc
    17221 cttggacact cgccacctat atggtggaag gtggttcaag caaagcattt accaaaatca
    17281 aaaaaatgct ttacgctgag cctcacattc tacacaaatt gctggacaag ttagctgaca
    17341 gtgtcatctt atacttaaat gcccaaatca aagccggtgc tcaagcggtg atggtattcg
    17401 acacttgggg cggtgtattg gctcacgagg attacaaagc cttctcatta cactatatgc
    17461 acaaaattgt ggacggctta attcgagaaa atgaaggacg taaagtgcct gttaccctat
    17521 tcaccaaagg cggaggctta tggttagaag cgattgcaga aacgggctgc gatgccgttg
    17581 ggctagattg gacagtcgat attgccgatg ctcgccgccg tattggcgac aaagttgcgt
    17641 tgcaaggcaa tatggatcca agcgtgctgt atgcaagccc tgaacgaatc gaacaagaag
    17701 taaaaaacat tcttgccggc ttcggcaacg gcagcggaca cgtctttaat cttggacacg
    17761 gcattcattt agatgtgccg gtggaaagcc cgaaagtgtt tgttgatgcg attcacaact
    17821 actcgaagca gtatcataaa taatggataa aatgccctct ctccttgtgg gagagggaca
    17881 gatttttctt tgttcagaag aaaaatcagg gagaggggca agcggtcatt tttatacaga
    17941 attttgcaaa atcgttccct tctctctgaa aaataaagaa ggaaatacaa tgagaaaccc
    18001 gattcataaa cgcatggaac gtttcgagcc ttggcaaaac accacattta tggcgtgcct
    18061 atgcgaaaga atgtacccca actatcagct tttttgtgaa gtaaccgaac agcctgaaaa
    18121 agctaaggta ttccaaaata ttttaaattt agtctgggaa tatttaacca taaaaggggc
    18181 aaaaattaat ttcgataacc aactggaaaa attagaagaa atcattcctg atgtgaacga
    18241 atatgcgttt tttggtgtct tacctgcaca ggaagcttgc gaagcccttt ctgaacttct
    18301 tcacagtatt atcgctggtt caacccttga acaagcaatc cgcatcagcc aaatttcatt
    18361 aggcacagtt gccagctatt tggaaatgca acaagataaa gaactgaacg agcaagagct
    18421 aaagaattct gaagaaattc aagaagaact ggacgtacag tggcaaatat accgcttgct
    18481 aaacgaatgt gaaaaacgtg atcttgatct catttttgaa cttaaagatg aaatcagaag
    18541 cagcgggatt agtaatatcg ggttaaatat taaccaatag cattgatatt tgcaaaatct
    18601 tgcttaataa tactttgaga aatcttaact ttgaattaag ctatcgcaag gtttcaataa
    18661 cttctcagac gttttacgtc atttattaaa aacacatatc ttagaggata aaactatgaa
    18721 caaaactgag ttaatcgatg ctatcgccgc tggtgcaaac ttaaccaaga aagatgctaa
    18781 agcagcatta gaagcaactc ttgatgcaat ctctgcaagc ttaaaagcag gcgacgctgt
    18841 tcaattaatc ggcttcggta cttttaaagt aaatgagcgt aaagcacgta ctggtcgtaa
    18901 cccaaaaact ggtgaagaga tcaaaattgc agcagctaaa gtacc
//
LOCUS       DANLXF010000023        18928 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.023, whole genome shotgun sequence.
ACCESSION   DANLXF010000023 DANLXF010000000
VERSION     DANLXF010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 18928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18928
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.023"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            23..523
                     /locus_tag="PXD66_000477"
     CDS             23..523
                     /locus_tag="PXD66_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL6318810.1"
                     /translation="MKKSLFVLSLISFALVGCSSNSGESTSAVETTEANATWQSADSI
                     QTAPMPASMNQPIATQPTYQQPTYSAPQPISAAPAPMPVSSGSDSSQVETVGNCQVVR
                     DANNAPIYSQITKGCYTESHYTVGKSDTLYLIGYLTGTNANQIAQLNGLNTGSPLKVG
                     QVLRVR"
     gene            536..736
                     /locus_tag="PXD66_000478"
     CDS             536..736
                     /locus_tag="PXD66_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318811.1"
                     /translation="MNRTLLAFAISCAFLTACSSPTQPQVEDPNMLPAGTLQPIEGSG
                     ASQDSYSWPSDIQSTSMPDSMK"
     gene            755..2035
                     /locus_tag="PXD66_000479"
     CDS             755..2035
                     /locus_tag="PXD66_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDL6318812.1"
                     /translation="MKKQFILLPLVSAVLAACSSNNPAPVVNASGNNELSPGVMQPVD
                     VSAGAINSSGGWQSDIQSAPMPSSMNTTPVQTMPQPVSVPQPVATVPQPITNPVITEQ
                     PPQPTTTTKIVKKTKTVEKKVDQNFEIPRDANNAPMYNQIQKGFYDGSTYTVRKGDTM
                     FLIAYIVGKDVKEIAALNNMSEPYQLSVGQKLKTGKSATETITVEEKVTVPVEPQITY
                     QQGANGTTYASGGNITGPVKAGTANADVTPVNNGIKATTATAPAVTASVGTVNATEDR
                     TASTIRAIDGTASGAVQSSNTVSAPASSIKWQWPTSGRVISGFSSAEGGNKGIDIAGN
                     KGQDVRAAAAGKVVYAGNALQGYGNLIIIKHTDDFLSAYAHNNTIEVDEQETVKAGQK
                     IATLGSTGTNTNKLHFEIRYKGKSVDPARYLPRK"
     gene            2276..2467
                     /locus_tag="PXD66_000480"
     CDS             2276..2467
                     /locus_tag="PXD66_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318813.1"
                     /translation="MDPTEPSHRIYILLSEDRFQAEVFSTKLSESSMLLEAVKGGYIS
                     KDGKVRLLKKAEGWKIYYE"
     gene            2460..3026
                     /locus_tag="PXD66_000481"
     CDS             2460..3026
                     /locus_tag="PXD66_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDL6318814.1"
                     /translation="MSETKIRCDWCKSSEIYMKYHDEEWGKPEFDSLKLFEKICLEGQ
                     QAGLSWITVLKKREAYRQAFHQFNPEKIAKMGEEEIDLLMQNTSLIHHRAKLEAIIKN
                     AKAYITMQQNGEDFSRFIWSFVNYQPQINDVPTLEAVPAHTETSKAMSKALKKRGFVF
                     VGETTCYAFMQSMGLVNDHLNNCVFKHK"
     gene            complement(3073..3303)
                     /gene="acpP"
                     /locus_tag="PXD66_000482"
     CDS             complement(3073..3303)
                     /gene="acpP"
                     /locus_tag="PXD66_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005725021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDL6318815.1"
                     /translation="MSIEERVKKIIVDQLGAKAEDVKPEASFIEDLGADSLDTVELVM
                     ALEEEFDIEIPDEEAEKITTVQSAIDYVQNNQ"
     gene            complement(3490..4167)
                     /gene="rpe"
                     /locus_tag="PXD66_000483"
     CDS             complement(3490..4167)
                     /gene="rpe"
                     /locus_tag="PXD66_000483"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDL6318816.1"
                     /translation="MSKNPYLIAPSILSADLARLGDDVAEVLKAGADLIHFDVMDNHY
                     VPNLTFGPAICKALRDYGIQCPIDVHLMVKPVERLIPDFAKAGADYITFHPEATEHID
                     RNLQLIRDLGCKSGLVFNPATPLSYLDYVMDKVDVVLLMSVNPGFGGQSFIPSTLDKL
                     KEVRRRIDESGYNIRLEVDGGVKINNIADIAAAGADMFVAGSAIFDQPDYQAVIAQMR
                     DELRKVG"
     gene            4361..4633
                     /locus_tag="PXD66_000484"
     CDS             4361..4633
                     /locus_tag="PXD66_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005709555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDL6318817.1"
                     /translation="MKKGIHPENYREVLFYDASVQQGWVIRSCAATNKTMVWEDGKEY
                     PLYTLDTSSASHPVYTGKRREVNTEGRASQFKDRFKGFASTLSNQK"
     gene            4642..4767
                     /gene="ykgO"
                     /locus_tag="PXD66_000485"
     CDS             4642..4767
                     /gene="ykgO"
                     /locus_tag="PXD66_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HDL6318818.1"
                     /translation="MKILNSLKTAKSRHPDCQIVRRKGKLYVICKTNPRFKARQR"
     gene            4947..5720
                     /locus_tag="PXD66_000486"
     CDS             4947..5720
                     /locus_tag="PXD66_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL6318819.1"
                     /translation="MLKKLSIAALALTLSVSAFAKESLLERINNKGTITVGTEGTYAP
                     YTYHDASGKLTGYDVEVTRAVAEKLDVKVEFKETQWDAMLAGLKGERFDLVANQVALT
                     SPERRATFDKSESYSWDGAALLVREDETKIKTPADVKGVKTAQTLSSNYGEIARENGA
                     DIVPIDGMAQGLLLIQQKRADATFNSHLSLLDYLKKNPNSGLKIVWEKEEKVGAGLIA
                     NKGNDEALAKISKAIVELREDGTLKKLGEQFFGKDISVQ"
     gene            5803..6519
                     /locus_tag="PXD66_000487"
     CDS             5803..6519
                     /locus_tag="PXD66_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL6318820.1"
                     /translation="MLNDLLLSIPFMTQARVDLVLSGFWPMVQAAFLVSIPLAIASFI
                     IGMLIAVAVALVRISPATGFFHRLLLWLVKGYISIIRGTPMLVQICIVFYGLPAIGIF
                     IDPIPAAIIGFSLNIGAYGSETIRAAISSVPKGQWEAGYTIGMTYMQTFRRIIAPQAF
                     RVAVPPLSNTFIGLFKDTSLASVVTVTEMFRVAQQVANMSYDFLPVYIEAGLIYWCFC
                     WVLFLIQARLEVRFNRFVAK"
     gene            6529..7281
                     /locus_tag="PXD66_000488"
     CDS             6529..7281
                     /locus_tag="PXD66_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL6318821.1"
                     /translation="MLKIRNIHKTFGNNAILRGIDLDIQKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEQGTLEFSGNAPLKIDFAVKQSKKEILALRRKSGMVFQNYNLFPHKTAL
                     ENVMEGPVFVQQQAVEIAQKNAKALLEKVGLADKAELYPFQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLNTMKELANEGWTMVVVTHEIKFALDVADVVVVMDG
                     GEIVEQGSPQQLFNHPQHERTKRFLNQIRADK"
     gene            complement(7497..8030)
                     /locus_tag="PXD66_000489"
     CDS             complement(7497..8030)
                     /locus_tag="PXD66_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDL6318822.1"
                     /translation="MEFLAPVISLIVGFLSFVLVLRTWLQFCRVDPRLPISQTLLKIT
                     EPMVSPVGKLIPTVKNICFPALLIAAALVTLQFILFGVDLPRAVLIGLLSVVKTFGQI
                     LFFTTLIRALMSWVTQGNHPLDYTVAQITEPVLGFIRKLLPRTGMLDFSVMVLGFGLL
                     LLNSLFYQIFGGLWAAA"
     gene            complement(8136..8477)
                     /locus_tag="PXD66_000490"
     CDS             complement(8136..8477)
                     /locus_tag="PXD66_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HDL6318823.1"
                     /translation="MAIQRNARQRKKMHLGEYQELGFLIKFTFAEGTGIDQIDETVDR
                     FIDEVIRPNGLALEANGYLNWEGLVCLEKLGKCDESHRQLVKTWLETNGLKEVQVSEL
                     FDIWWDYPAQA"
     gene            complement(8501..9250)
                     /gene="trmB"
                     /locus_tag="PXD66_000491"
     CDS             complement(8501..9250)
                     /gene="trmB"
                     /locus_tag="PXD66_000491"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDL6318824.1"
                     /translation="MSEKQTFADKKRKTVEQAEFTEDGRYLRKVRSFVLRTGRLSDYQ
                     RDMMNNNWANLGLDYQPMPFNFEQIFGNNNPVVLEIGFGMGKSLVEMAEQNPNRNYIG
                     IEVHTPGVGACIAYALEKGVKNLRVICHDATEILRDSIADGTLGGLQLYFPDPWQKAK
                     HHKRRIVQPEFISRVIRKLSPNGFIHFATDWENYAEHMLEVLRQFESELQNTSATNDF
                     IPRPNFRPLTKFEERGHRLGHGVWDLYFTKK"
     gene            complement(9260..10210)
                     /locus_tag="PXD66_000492"
     CDS             complement(9260..10210)
                     /locus_tag="PXD66_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDL6318825.1"
                     /translation="MNSRILATGSYLPTKVRTNADLEKMVDTSDEWIVTRSGIRERRI
                     AEPDETVATMGAKAAQQALEMAGIEANDIDLIVVGTTTNSHAYPSAACQIQGLLQIED
                     AIAFDVAAACTGFVYALTVADQFVRNGQVKKALVIGSDINSRMLDETDRSTVVLFGDG
                     AGAVILEASEELGIISTHLHASPDLENNLTLRSQKRGEERSGFIEMQGNATFKIAVNQ
                     LSSVVEETLEANNLQKSDLDWLIPHQANLRIITATAKKLEMEMDQVVVTLDKYANTSG
                     ATVPVALDEAVRDGRIQRGQLLLLEAFGGGWTWGSVLVKF"
     gene            complement(10356..11375)
                     /gene="hemB"
                     /locus_tag="PXD66_000493"
     CDS             complement(10356..11375)
                     /gene="hemB"
                     /locus_tag="PXD66_000493"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDL6318826.1"
                     /translation="MQVIQSQYPLRRMRRLRKHDFSRRLVAENQLTVNDLIYPVFVIE
                     GENQRVQVPSMPGVERLTIDQLLVEAAELVKYGVPAVALFPVVGDVKKSLMAEEAYNP
                     QGLAQRAVRALKAQFPELGVITDVALDPFTTHGQDGIIDETGYVLNDVTTEVLVKQAL
                     SHAEAGADIVAPSDMMDGRIGKIREALEQNGFINTQIMAYSAKYASNYYGPFRDAVGS
                     AGNLKGGNKYTYQVDPANGNEGLHEVAMDIQEGADMVMVKPGMPYLDMVWRVKENFGV
                     PTFAYQVSGEYAMHMAAIQNGWLKERECIMEGLLCFKRAGADGILTYFAKRVAKWLYE
                     EAQAK"
     gene            complement(11456..12211)
                     /gene="tatC"
                     /locus_tag="PXD66_000494"
     CDS             complement(11456..12211)
                     /gene="tatC"
                     /locus_tag="PXD66_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HDL6318827.1"
                     /translation="MSQVDESQPLITHLIELRNRLLRSFICVLLVFCGLVYWANDIYT
                     LLATPLTDNLPDGATMIATNVATPFFTPIKLTIVVSVFLSVPFILYQIWAFVAPALYK
                     QEKRLVYPLLVSSTLLFYCGVAFAYYVVFPLVFGFLTSTAPEGVTMATDISSYLDFVL
                     TIFMAFGICFEVPVAIILLCWAGVTTPEDLKAKRPYIIVAAFVIGMLLTPPDIFSQTL
                     LAIPMCLLFEVGVLFARFYRPKDDDEPSEQVAQ"
     gene            complement(12198..12842)
                     /gene="tatB"
                     /locus_tag="PXD66_000495"
     CDS             complement(12198..12842)
                     /gene="tatB"
                     /locus_tag="PXD66_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HDL6318828.1"
                     /translation="MFDIGFSELVLIMIVGLVVLGPKRMPIAIRTVMGWVSTIRGLAA
                     NVQNELAQELKLQELKESIKKAEELNLSSLSPELNKTVEDLKMSATQLQDSLNSAKNE
                     LEKTAAESSTIPKLTDEQVAEIQQKIEQESQVLEAQELQKNAQNKPLEVDSLSEQLNV
                     TRTEKPLSADELAEQAEMDESLLTLESYYPPDDDLATPQSSESAQKSEANNVAS"
     gene            complement(12846..13067)
                     /gene="tatA"
                     /locus_tag="PXD66_000496"
     CDS             complement(12846..13067)
                     /gene="tatA"
                     /locus_tag="PXD66_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HDL6318829.1"
                     /translation="MGGISIWQLLIVVAIIVLLFGTKKLRTLGSDLGESVKGFKKAMA
                     DEKKDAEFEKVEAKDAISAEQKAKEKEQA"
     gene            complement(13178..14815)
                     /gene="ubiB"
                     /locus_tag="PXD66_000497"
     CDS             complement(13178..14815)
                     /gene="ubiB"
                     /locus_tag="PXD66_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HDL6318830.1"
                     /translation="MNIKNLNRFYHIIRTFLRYGIDEAIPNLPITRKIKLGRKALFWV
                     KNQHSDKPYGVRLRLALQELGPVWIKLGQMLATRRDLFAPELADQLALLQDNVEPFDG
                     KIARQIIEQALGGKLETWFENFDETALASASIAQVHTAKFNQNQPLAGKDVVLKVIRP
                     GIEPVIKADLELMYEIAHFIPKLSNDGKRLRAVEVVREYEKTILDELDLRKEMHNAIR
                     LRANFENSEMLYVPEMYQDFCHKNLIAMERIYGIPVSNIAELEANSTDMKLLAERGVQ
                     VFFTQVFRDSFFHADMHAGNIFVNREHPENPQYIGIDCGIVGTLNHNDKRYLAESFVA
                     FFNRDYRRVALMHVESGWTPPDTDIDKFEQAFREVCEPIFAKPLSEISFGHVLLNLFN
                     VAREFNMEVQPQLVLLQKTLLYIEGLGRQVYPQLDLWQTAKPFLQNWLNEQVGVKAMV
                     RDIKQRLPQYREHFAEFPEALFKALQEQKQINFRLAEINESLKAQNSNANSRFLVLSI
                     SAIVICGAFWKFESLPIWLSVPLLTLFPLVLILSQRK"
     gene            complement(14889..15866)
                     /locus_tag="PXD66_000498"
     CDS             complement(14889..15866)
                     /locus_tag="PXD66_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005825116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HDL6318831.1"
                     /translation="MKQIIAQARENAVRAVDFQRVLMYWHIGKRIFEQEQQGQERADY
                     GTYLIKMLAKELEPEFGSGFCSRQLERYRQFYRAFPIATALRSQLNWTQYRRLIQISD
                     SDKREFYLAESCKNNWSSRQLERQINSSLYERLLLSNDKESVLAVARNEQQPVDPKQL
                     IKDPMVLEFLGLKRESAYYEKDLEQAIITHLQDFLLELGNGFSFIARQKRLHLDGDDF
                     FVDLVCYNRLLQCFVIFEIKTHKLTHQDLGQLQMYVNYFDRVEKLPHENATIGVLLCA
                     DKNDAVVKFSLPENQNKIFASQYQLYLPSEQTLIAEVQKEIENFEQKYP"
     gene            complement(16027..16674)
                     /locus_tag="PXD66_000499"
     CDS             complement(16027..16674)
                     /locus_tag="PXD66_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HDL6318832.1"
                     /translation="MLNNVKQQLMLPQFTFGLIETAFNALLKRSPHIEPNLRKLSGKV
                     LKIALTSPKIDFFVLFSESRTDWLGNYEGEADCTVQLAFDTLPKLADKQKLTELINNK
                     SLVLTGDIQVLQHFTALLEQLEKDPAELLSPFVGDVVAQTSTDLAKKLLGKVKAQFEQ
                     NNQHLVENLIQERPVLVHRLQAVHFYDQVEELAQQALKLEQKFTHYVRSSSHQQS"
     gene            complement(16865..17617)
                     /gene="ubiE"
                     /locus_tag="PXD66_000500"
     CDS             complement(16865..17617)
                     /gene="ubiE"
                     /locus_tag="PXD66_000500"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HDL6318833.1"
                     /translation="MQQSAETTHFGFKTVAKEEKQQLVANVFHSVAGKYDLMNDLLSF
                     GIHRVWKRFTIDCSGVRKGQKVLDLAGGTGDFTAKFSRIVGESGEVILADINSSMLEV
                     GREKLRNLGVVGNVSYVQANAECLPFADNTFDCIVISFGLRNVTDKDKALRSMFRVLK
                     PGGRLLVLEFSKPIIDPISQLYNFYSFNILPKVGEVVVNDAESYRYLAESIRMHPKQD
                     ELKAMMEQTGFESVNYYNLSAGIVALHRGYKF"
     gene            17962..18915
                     /gene="corA"
                     /locus_tag="PXD66_000501"
     CDS             17962..18915
                     /gene="corA"
                     /locus_tag="PXD66_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HDL6318834.1"
                     /translation="MIRAFGLDNACLISVDETNPTLLNDAIWIDLIDPSEAERTVLEN
                     RLDQTLAEERELEDLEASARFFEDEDGLHLHSFFYCLDDDDYADIATVAFTIRDGRLF
                     TLRDRDLPAFRLYRMRSRREKLIDSNAYELLLDLFETKIEQLADVIEEVYADLEKLSR
                     VILNGQQENENFNDALSRLTEFEDASSKVRLCLMDTQRALSFLLRKTRLPNNQLEQAR
                     DIMRDIESLQPHNESLFQKVNFLMQAAMGYINIEQNRIMKFFSVVSVMFLPATLVAST
                     YGMNFEFMPELHFQYGYPMAIGLMIVAASAPYLYFKRKGWL"
BASE COUNT         5543 a         4190 c         3678 g         5517 t
ORIGIN      
        1 aataaatttt aaggatataa aaatgaaaaa atcacttttt gttttatctc tgatttcttt
       61 tgctttagtg ggttgttcaa gcaattcggg tgaatccact tctgcggtag aaacaacaga
      121 agcaaatgca acttggcaat cagcagatag cattcaaact gcaccaatgc ctgcctcgat
      181 gaatcaacct attgccactc agccaaccta tcagcagccg acttactctg caccacaacc
      241 aatttcggca gcaccagcac caatgcctgt ttcaagtggt agtgatagct ctcaagttga
      301 aacagtaggg aactgccaag tggtgcgtga tgcgaataat gctcctatct attcacaaat
      361 taccaaaggc tgctacacag aaagtcacta tacggtaggt aaaagtgata cgctttattt
      421 aatcggttat ctaaccggca ccaatgccaa tcaaattgcg cagctaaatg gtttaaatac
      481 cggctctcca ctgaaagtcg ggcaagtctt acgtgttcgt taataggcag agaaaatgaa
      541 cagaaccctt ttagcatttg caatttcttg tgcatttttg accgcttgta gctcgcctac
      601 tcagccacaa gttgaagatc caaatatgtt acctgcaggc acattacaac cgattgaagg
      661 ctcaggcgca agccaagaca gttacagttg gccgtctgat attcaatcaa cgtcaatgcc
      721 tgacagtatg aaataatttt aggacaatca tttaatgaaa aaacaattta ttcttctccc
      781 tttagtctct gctgttttgg cagcctgttc ttcaaacaat ccagcaccgg tggtgaatgc
      841 ttccggtaat aacgaattaa gcccgggcgt gatgcagcct gttgatgtga gtgcaggtgc
      901 aattaattca agcggtggct ggcagtctga tattcaatct gcaccaatgc caagctcgat
      961 gaataccact ccggtgcaaa caatgcccca gccggtttca gtgccacaac ctgtggcgac
     1021 tgtaccgcag ccaattacca atccggtgat taccgaacaa ccaccacagc cgacaaccac
     1081 gaccaaaatc gtgaaaaaaa cgaaaacggt cgagaaaaaa gtggatcaaa attttgagat
     1141 cccgcgtgat gccaacaatg ccccaatgta caaccaaatt caaaaagggt tctacgatgg
     1201 ctctacctat accgtacgca aaggcgacac aatgtttttg attgcctata ttgtgggtaa
     1261 agatgtaaaa gaaatcgccg ctcttaataa tatgagcgaa ccttaccaat taagcgtagg
     1321 gcaaaaactc aaaaccggca aatctgcaac ggaaactatt accgtggaag aaaaagtcac
     1381 cgtaccggtt gaaccacaaa ttacctacca acaaggtgca aacggcacaa cctatgcttc
     1441 tggcggtaat attactgggc cggtaaaagc aggcacagcc aacgctgatg tcacgccggt
     1501 taataacggt attaaagcaa caactgcaac tgctccggca gtaactgctt ctgtcggtac
     1561 agtaaatgca acagaggacc gtaccgcctc caccattcgt gctatagatg gcacagcaag
     1621 tggtgccgta caaagtagta atactgtttc tgcaccagct tcatcaatta aatggcaatg
     1681 gccaaccagc gggcgtgtaa tttcaggctt ctcctcagca gaaggaggta ataaaggcat
     1741 tgatattgcc ggcaataaag gacaagatgt acgtgctgcc gccgcaggta aagtggtata
     1801 tgccggtaat gccttgcagg gttacggtaa cttaatcatc atcaaacata ccgatgattt
     1861 cttaagtgct tatgcacaca acaatactat tgaagttgat gagcaagaaa cggtgaaagc
     1921 cggtcagaaa atcgcgactt taggtagtac aggaaccaat acgaataaat tacattttga
     1981 aattcgttac aaaggaaaat ctgtcgatcc ggctcgttat ttaccaagaa aataagagta
     2041 actttttagc ataatccgac cgcttgtgta ctacaagcgg tcaattttta ggaaaattat
     2101 gcaaatgaaa aaatatatcg cacacatgat aagttcgtta ctgctgatag tttgttcgga
     2161 ctaccgagca acaccaacta gcccaagcga tgcttacact tgctctcctt cttttcgcta
     2221 ctctttctta aaaaaaacgt gcataccttt attcagtata gccgacattc aattattgga
     2281 tccaaccgag ccttcacatc gcatttatat tttgttatct gaagatcgat ttcaagccga
     2341 agtcttctca acgaagctct ctgaatcttc tatgttattg gaggcggtta aaggaggcta
     2401 tatttcaaaa gacggaaaag ttcgtttact caaaaaagct gaaggttgga aaatttacta
     2461 tgagtgagac taaaatacgt tgtgattggt gtaaaagcag tgaaatttat atgaaatatc
     2521 acgacgaaga atggggaaaa ccggagtttg acagcctcaa actgtttgag aaaatctgcc
     2581 ttgaagggca acaagccgga ctttcttgga ttacggtgtt gaaaaaacgg gaagcctacc
     2641 gccaagcatt ccatcagttt aatcctgaaa aaattgccaa aatgggtgaa gaagaaattg
     2701 atttgttaat gcaaaacact agcttaatcc accaccgagc aaagttagaa gccattataa
     2761 aaaatgcgaa agcttatatc acaatgcagc aaaatggcga agacttcagt cggtttattt
     2821 ggtcttttgt gaattatcag ccgcaaatta atgatgtacc aacacttgaa gctgttcctg
     2881 ctcatacaga aacctcaaaa gcaatgtcaa aagcgttgaa aaaacgtggg tttgtgtttg
     2941 taggcgagac tacttgctat gcttttatgc agtcaatggg gctggttaat gaccatttga
     3001 ataattgcgt gttcaaacat aaataaacac aaaaaattag gcgaccattt ggtcgcctaa
     3061 cattaattca gattattgat tgttttgaac gtaatcaatt gctgattgaa ctgtagtaat
     3121 tttttcagct tcttcatctg gaatttcgat atcgaattct tcttctaaag ccattactaa
     3181 ttcaacagtg tctaaagaat ctgcacctaa atcttcaatg aatgaagctt ctggttttac
     3241 atcttctgct ttcgcgccaa gttgatcaac aatgattttt tttacgcgtt cttcaatgct
     3301 cattttgttt ttcctatagt gaatatcgca taagtgcgag agttaaatag tgtaatcgaa
     3361 ataagtttaa atacaacctt ttataaaaag gtcaaaccag actattccta tacaccagaa
     3421 tatatctgtt tttccattct atcattatgt ttgtgtttaa tctatagaga taaatcaaat
     3481 ttcatttgat taccctactt tccgtaattc atctcgcatt tgtgcaatca ctgcttgata
     3541 atctggctgg tcaaaaatcg cagaacctgc cacaaacata tctgctcctg cggctgcaat
     3601 gtcggcaatg ttatttattt tcacaccacc atcaacctct aagcgaatgt tataaccgct
     3661 ctcatcaata cgacgacgga cttctttcaa tttatctaag gtggacggaa taaatgattg
     3721 tccgccaaag cccggattca cagacatcaa taaaaccacg tcaactttat ccatcacata
     3781 atctaaatag ctcaatggcg ttgccgggtt aaacaccaag cctgatttac aacctaaatc
     3841 acggataagc tgtaaatttc gatctatatg ttccgttgct tcagggtgga atgtaatata
     3901 atccgctccg gctttggcaa aatccggaat caaacgctca accggtttta ccattaaatg
     3961 cacatcaatc gggcattgaa tgccgtaatc acgtaaggct ttgcagatcg ccggcccgaa
     4021 ggtcaaattc ggcacgtagt ggttgtccat tacatcaaag tgaattaagt ctgcaccggc
     4081 ttttaacacc tctgccacat catcacccaa gcgagctaaa tcggcagata aaatagacgg
     4141 tgcaattaaa taaggattct tactcatatt tcctcactat caaaaaataa atctacgtta
     4201 gattaccata attttaacag cgaaaagagt ctctcttcct aaaaatccgt ttttttacaa
     4261 aacaccataa aaaagttatg gaattgtatt taaagttcgt atattatgta tcccatattt
     4321 taattatggt taatgacctt atctaaattc tggagaaaat atgaaaaaag ggattcatcc
     4381 tgaaaattat cgtgaagtgt tgttctatga tgcctcagtg caacaaggct gggttatccg
     4441 ttcttgtgcg gcaaccaaca aaacaatggt atgggaagat ggcaaagaat acccacttta
     4501 caccttagat acctcatcgg cgtctcaccc tgtttacaca ggtaaacgcc gtgaagtgaa
     4561 tacagaaggt cgtgcaagtc aatttaaaga tagatttaaa ggctttgctt caacgctttc
     4621 aaatcaaaaa tagaggataa aatgaaaatc ttaaactcac ttaaaaccgc aaaatcacgc
     4681 cacccggatt gccaaattgt cagacgcaaa ggcaaacttt atgtgatttg caaaaccaac
     4741 ccgagattta aagctcgtca gcgttaaaaa ccagcacctt aataagctaa tttattaagg
     4801 tgttttttat ttcatcttct caatattacc tattttaacc acatcaacga ttttattatt
     4861 tcttattcca tttagttatt tgcaagcggt caattttccc cttaaatttg caaaaaattt
     4921 attttaccaa ttaaaaggaa atgattatgt tgaaaaaatt atcaattgct gcattagctc
     4981 ttaccctttc tgtttctgca tttgctaaag agagcctact tgagcgtata aacaacaaag
     5041 ggactattac tgtgggtacg gaaggaactt atgcccctta cacctatcac gatgcgagcg
     5101 gtaaattaac cggttacgat gtagaagtaa cccgtgcggt ggctgaaaaa ttagacgtaa
     5161 aagtggaatt taaagagaca caatgggacg ctatgttagc cggtttaaaa ggtgagcgtt
     5221 ttgacttagt ggcaaaccaa gtggctttaa ccagcccgga acgtcgagcg acatttgata
     5281 aatccgaatc ttacagctgg gacggtgccg ccttattagt gcgtgaagat gaaactaaaa
     5341 tcaaaacccc tgctgatgta aaaggcgtga aaactgccca aaccttgagc tcaaactatg
     5401 gcgagattgc ccgtgaaaac ggtgcagaca tcgtgccaat tgacggtatg gctcaaggct
     5461 tactgttaat tcaacaaaaa cgcgccgatg caactttcaa tagccactta tcgctattag
     5521 attacttgaa aaagaacccg aattccggct taaaaattgt gtgggaaaaa gaagaaaaag
     5581 tgggtgcagg cttaattgcg aataaaggca atgacgaagc attagcgaaa atcagcaaag
     5641 caatcgtaga attacgtgaa gacggcacgt taaaaaaatt gggcgagcag ttcttcggta
     5701 aagatattag tgtacaataa tccatattgg attaggcttg gggcgaattc gcccctttta
     5761 tttttaaatc tccctcaaaa cgggagattt taggtttttc agatgttaaa cgatctactt
     5821 ctctcaatcc cctttatgac ccaagctcgg gtcgatttag tgctaagcgg tttttggccg
     5881 atggtacaag cggcattttt agtctctatt ccgctggcaa tcgcctcatt tattatcggt
     5941 atgttaattg ccgttgcagt tgcattagtg cgaattagcc ctgcaacagg cttttttcac
     6001 cgcttgctac tgtggctggt aaaaggttat atctcgatta ttcgcggcac gccgatgttg
     6061 gttcagatct gtattgtgtt ttatggtttg cctgcaatcg gcatttttat cgatccgatt
     6121 ccggcggcaa ttatcgggtt ttcactcaat atcggggctt atggctcgga aacgatccgt
     6181 gctgcgatct cctccgtgcc aaaaggacaa tgggaagccg gttatactat cggtatgact
     6241 tatatgcaaa cttttcgccg tatcattgcc ccgcaagcgt ttcgagttgc tgttccgcca
     6301 cttagcaaca cctttatcgg tctgtttaaa gatacctctc ttgcctccgt tgttaccgta
     6361 accgaaatgt tccgggttgc tcaacaggta gcaaatatgt cttatgactt cctacctgtt
     6421 tatattgaag ccggtttgat ttattggtgc ttctgctggg tgctattttt aattcaggcg
     6481 agattggaag tccgctttaa ccgatttgtg gcgaaataag gaggaattgt gcttaaaatt
     6541 cgcaatattc ataaaacctt tggcaacaac gctattctac gcggcattga tttagatatt
     6601 caaaaagggc aagtggtagt aattttaggc ccttccggtt caggcaaaac cacctttctt
     6661 cgttgcttaa atgcactgga aatgcccgag caaggcacat tggaatttag tggtaatgct
     6721 ccgctgaaaa ttgattttgc cgtaaaacag agcaaaaaag agattttagc gctacgccgc
     6781 aaatccggta tggtgttcca aaactacaac ctgttcccgc ataaaaccgc attggaaaat
     6841 gtgatggaag gaccggtatt tgtgcaacaa caagcggtcg aaattgctca aaaaaatgca
     6901 aaagccctgt tagaaaaagt cggactggcg gataaagcag aactctaccc gtttcaactg
     6961 tcaggcgggc aacagcaacg ggttggcatt gcacgagcat tagcaattca gccggagctg
     7021 atgttatttg atgaaccaac ttcggcactt gatcctgagt tagtgcaaga cgtgctaaat
     7081 acgatgaaag aactggcaaa tgaaggctgg acaatggtcg tggttactca cgaaatcaaa
     7141 tttgcccttg atgttgccga tgtagtggtg gtaatggacg gcggtgaaat cgttgaacaa
     7201 ggctcgccac agcaactgtt taaccaccca caacacgaac gaactaaacg ttttttaaat
     7261 caaatcagag cggataaata agaaacgata gcgctagcgt ccacgctagt gctatctaat
     7321 aaatggtaca agcggttaat tatgtataaa ttgttatact gttttggcac gagcgtggac
     7381 gctcgcgcca tcgtacagta aaatcagcta acaaggttcc ccataaagga taaataagac
     7441 aaggctcctt aatcttttag attaaggagc ttatttttag attttagaaa tccgatttaa
     7501 gccgcagccc atagtccacc aaaaatttgg tagaacaagc tatttagtag caataagccg
     7561 aaacctagca ccattaccga gaaatctaac attcccgttc ttggcaggag cttgcggata
     7621 aaacctaaca ccggttcggt aatttgtgct accgtgtagt ctaatgggtg gttgccttga
     7681 gtgacccagc tcattaatgc acgaatcaag gtggtgaaaa atagaatttg tccgaaggtt
     7741 ttgaccacac ttaataagcc gattaatact gctctgggta aatctacgcc aaacagaata
     7801 aactgtaacg ttaccaaagc ggcggcaatt aatagtgccg gaaagcaaat atttttaacg
     7861 gttggaatca gcttgccaac cggtgaaacc atcggttcgg taattttaag taaggtttgt
     7921 gaaatcggta aacgaggatc aacccgacaa aattgcagcc aagttcttag caccagcaca
     7981 aaacttaaaa agccaacaat aagcgaaata acaggagcta aaaattccat ctctttttct
     8041 ctttctatac aaaacaagcg gtcgtttttt agaaaaaatt tgcaaatttt cagtaaaatc
     8101 tgaccgcttg tatcttatta aattaactag gcaaattaag cttgtgctgg gtagtcccac
     8161 caaatatcaa ataattcgct cacttgaacc tcttttaagc cgttagtttc taaccaagtt
     8221 ttcactaatt ggcggtgaga ttcatcacat ttgcctaatt tttctaagca gactaaacct
     8281 tcccagttta agtagccgtt cgcttcaagg gctaaaccgt ttggtcgaat cacttcatcg
     8341 ataaaacgat ctacggtttc atcaatttgg tcaatgcctg tgccttcagc aaaggtgaat
     8401 ttaattaaga aacctaattc ttggtattca cctaagtgca tttttttacg ttggcgtgcg
     8461 ttacgttgaa tagccatttt ttctctcctc tatgaataaa tcatttcttt gtaaaatata
     8521 aatcccacac cccgtgtcct aaacgatgcc ctcgctcttc aaatttggtt agcgggcgga
     8581 aattcggacg tggaataaaa tcattagttg ctgatgtatt ttgcaactcg ctctcaaatt
     8641 ggcgtaatac ttcaagcata tgttctgcgt aattttccca gtcggtggca aaatgaataa
     8701 agccgttagg gcttaatttt ctgatcacac ggctgataaa ttcaggctgc acaatacggc
     8761 gtttgtggtg ttttgctttt tgccaagggt ctgggaagta aagctgtaag ccacctaatg
     8821 taccgtcagc aatgctgtct cgcaaaattt cggtggcatc gtggcaaatt actcttaaat
     8881 tcttcacgcc tttttctaac gcataagcga tacaagcccc caccccgggc gtgtgcacct
     8941 caatgccgat atagttgcga ttcgggtttt gctctgccat ttccaccagc gattttccca
     9001 ttccgaagcc gatttctaac actaccgggt tgttattgcc gaaaatctgc tcaaaattaa
     9061 agggcattgg ctggtaatct aagcctaaat ttgcccagtt gttgttcatc atatcacgtt
     9121 gataatcgct caaacgaccg gtacgcaata caaaactccg cacttttcgt aaataacgcc
     9181 catcttcggt aaactcggct tgctcaacgg ttttacgttt tttatctgca aaagtctgtt
     9241 tttctgacat tttcttgcct taaaacttca ctaataccga accccaagtc cagccaccgc
     9301 cgaatgcttc aaggagtagt aattgcccac gttgaattcg cccgtcacgc accgcttcat
     9361 ctaatgccac cggcactgtt gctccactgg tattggcata tttatcaagg gtaaccacca
     9421 cttgatccat ttccatctct aattttttcg ccgttgcggt gataatgcgt aaattcgctt
     9481 ggtgcggaat cagccaatct aaatcggatt tttgtagatt attcgcttct aaggtttctt
     9541 ctaccacgct tgaaagttgg tttactgcaa ttttaaaggt ggcattgccc tgcatttcaa
     9601 taaagccgga acgctcttcg ccacgctttt ggcttcttaa ggttaagtta ttttctaaat
     9661 ccggtgaggc gtgcaagtgg gttgaaataa tacccagctc ttcactcgcc tctaaaatca
     9721 ccgccccagc tccgtcacca aataatacta ctgtgctgcg gtcggtttcg tctaacattc
     9781 tggaattgat gtctgaacca ataaccaatg cctttttcac ttgcccgtta cgcacgaatt
     9841 gatcggcaac agttaaagca tacacaaatc ctgtacaagc cgctgccaca tcaaaagcaa
     9901 tcgcgtcttc aatctgaagc aagccttgaa tttggcaagc tgcacttggg taagcgtggg
     9961 agttggtagt tgttcccacc acaattaaat caatgtcatt tgcctcaatt cctgccattt
    10021 ccagtgcttg ttgagcggct tttgccccca ttgttgccac tgtttcgtca ggctcggcaa
    10081 tacggcgttc acgaatgccg gaacgggtta cgatccattc atcagaggta tcgaccattt
    10141 tttctaaatc agcattggta cgcacctttg tcggtaaata gcttcccgtt gctaaaattc
    10201 tactattcat atttttatta ttctcaatca attaaatgat aatccgttat tatagcttga
    10261 aaaccaacaa atatataggc tttacaagcg gtcattttta gcaaaaaagt tgcattcacg
    10321 aacgattttg cactagaacc accgcttgct agtatttatt tcgcttgagc ctcttcgtat
    10381 agccacttcg ctactcgctt ggcgaaataa gttagaatac cgtctgctcc tgcccgttta
    10441 aagcagagta atccctccat aatgcactca cgctctttca gccagccgtt ttggattgcc
    10501 gccatatgca tcgcgtattc gcccgaaact tggtaggcga aggttggcac gccaaaattc
    10561 tctttcacac gccacaccat atccaaataa ggcatacccg gtttcaccat caccatatct
    10621 gccccttctt gaatatccat cgccacttcg tgcaagcctt cgttgccgtt agccggatcg
    10681 acttggtaag tgtatttatt accgcctttt aagttgccgg cagaacctac tgcatcacgg
    10741 aacggtccgt aataattaga cgcatattta gccgaatacg ccataatttg cgtattgata
    10801 aaaccgtttt gctcaagggc ttcacgaatt ttgccgattc gaccgtccat catatcgctc
    10861 ggggcaacaa tatccgcccc cgcttctgcg tgcgataacg cctgttttac caatacttca
    10921 gtagtcacat cattcagtac atagcctgtt tcatcaataa tgccgtcttg tccgtgcgtg
    10981 gtaaacgggt ctaacgccac atcagtaatc acgcctaatt ccggaaattg agcttttaaa
    11041 gctcgcacgg cacgctgtgc caacccttgc gggttatagg cctcttctgc catcagtgat
    11101 tttttcacat cacccaccac agggaaaaga gccaccgcag gcacgccata tttaaccagt
    11161 tctgcggctt caactaaaag ctggtcgata gttaaacgct ctacacccgg catagaaggc
    11221 acttgtactc gctgattttc cccttcgatg acaaaaacag ggtaaatcaa atcattaacg
    11281 gtaagttgat tttctgccac taagcggcgg ctgaaatcgt gcttgcgtaa acgacgcata
    11341 cggcgtaaag gatattggga ttgaattact tgcattttta atttcctaat tcttaaaata
    11401 cctattcttg aaataagaaa ggcatagcaa acaggtgcta tgccttttca aaatattact
    11461 gtgcaacctg ctctgatggt tcatcatcat ctttggggcg ataaaaccga gcaaatagca
    11521 caccaacttc aaacaataaa cacattggaa ttgccaataa tgtttgcgag aaaatatctg
    11581 gtggggtgag caacatacca attacaaatg ctgccacaat aatataaggg cgttttgcct
    11641 ttaaatcttc gggtgtggtt acgcctgccc aacataacaa gataatcgca accggcactt
    11701 caaagcagat cccgaatgcc ataaaaatgg ttaaaacgaa atcaagatag ctactaatat
    11761 ccgttgccat tgttacccct tccggagcgg tagaggttaa aaagccaaat acaagcggaa
    11821 atacaacata ataggcaaaa gcgacaccac aataaaacag caatgtgcta gataccaata
    11881 atgggtaaac caaacgcttt tcctgcttgt ataaagcagg ggcgacaaac gcccaaattt
    11941 ggtacaaaat aaaaggaact gataaaaaca ccgaaaccac aatggttaat ttaattgggg
    12001 taaaaaacgg agtggctaca ttggttgcaa tcattgttgc accgtctggt aaattgtccg
    12061 ttaaaggtgt tgccaataag gtgtaaatat cattggccca gtaaaccaat ccgcaaaaca
    12121 ccaagagcac acagataaaa ctacgcagta atcggtttct cagctcaatt aaatgtgtaa
    12181 tcagaggttg agactcatca acttgcgaca ttatttgcct ccgatttctg cgctgattct
    12241 gatgattgag gcgtagctaa atcatcatct ggcggatagt aagattccaa cgttaaaagc
    12301 gattcgtcca tttctgcttg ttcagccagc tcatcagccg aaagtggctt ttctgtccgc
    12361 gtaacattaa gttgctcaga taaactatcc acttcaagcg gtttattttg cgcatttttt
    12421 tgcaattctt gcgcctctaa cacctggctt tcttgctcga ttttttgctg aatttctgcg
    12481 acttgctcat cggttaattt aggaattgtg ctgctttccg cagccgtttt ttccaactca
    12541 ttttttgcag aattaagcga atcttgcaac tgggtggctg acattttcag atcttccacc
    12601 gttttattta attctggcga aagggagctt aaattcaact cttctgcctt tttaatgctc
    12661 tcttttaatt cttgcagttt cagctcttga gccagttcat tttgtacgtt tgcggctaaa
    12721 cctcgaattg tactcaccca gcccattacg gtacgaatcg caatcggcat acgtttcggg
    12781 cctagcacca ccaagcccac aatcataatc agtactaatt cagaaaaacc tatatcaaac
    12841 acggattaag cctgctcttt ctcttttgct ttctgctctg ctgaaattgc atcttttgct
    12901 tccacttttt caaattcagc atcttttttc tcatctgcca ttgctttttt gaagcctttt
    12961 acagattcgc ccaaatccga acctaaagta cgtaatttct tcgtaccgaa aagtaatacg
    13021 atgattgcaa ccacaatcag aagctgccaa atactaatac cgcccataaa aactcctttc
    13081 atttaatagg gaatagataa aataaaacgc tgtttattat atgttacttt gacatataaa
    13141 aaagtaattt tgcaaaaaat ttgtcaaaaa caaccgctta tttgcgttga ctcaaaatca
    13201 ataccagtgg aaataaagtt aataacggca cacttagcca tatcggcaaa gattcaaatt
    13261 tccaaaatgc gccacagatc acaatcgctg aaattgacag cacaagaaaa cggctatttg
    13321 cgtttgaatt ctgtgctttt aaactttcgt taatttccgc taaacggaaa ttaatttgct
    13381 tttgctcttg taaggcttta aacaatgcct ccggaaactc cgcaaaatgc tcccgatatt
    13441 gcggtaaacg ttgcttaatg tctcgaacca tcgctttcac acctacctgc tcattcagcc
    13501 agttttgtaa aaacggtttt gcggtttgcc ataaatccaa ctgtggataa acctgcctgc
    13561 ccaaaccttc aatataaaga agtgtttttt gcaacaacac caactgcggc tgcacttcca
    13621 tattaaattc tcttgccaca ttaaacaggt tgagcaaaac gtgtccgaat gaaatttccg
    13681 ataacggctt agcaaaaatc ggctcacata cctcacgaaa ggcttgctca aatttatcaa
    13741 tatcggtatc cggcggcgtc cagcccgatt ccacgtgcat taaggcaaca cggcggtaat
    13801 ctcggttgaa aaatgcgaca aaactttcgg ctaaatagcg tttgtcattg tggttcaacg
    13861 tgccgacaat cccgcagtca atgccgatat attgcggatt ttccgggtgt tctctgttca
    13921 caaaaatatt gccggcgtgc atatcggcgt ggaaaaaact gtcacggaat acttgggtaa
    13981 aaaatacctg cacaccacgc tctgccagca atttcatatc ggtgctgttc gcttccaact
    14041 cggcaatgtt agacactgga atgccataaa tccgctccat cgcaatcaag tttttatggc
    14101 aaaaatcttg atacatttca ggcacataca gcatttcgct attttcaaaa ttcgcccgaa
    14161 gccgaatggc attgtgcatt tctttgcgta aatccagctc gtctaaaata gttttttcgt
    14221 actctcgcac cacttcaacc gcccgtagcc gtttgccgtc attcgataat ttcgggataa
    14281 agtgagcaat ttcatacatc aactctaaat ccgccttaat caccggctca atgcccgggc
    14341 gaatcacttt taacaccaca tctttgcctg caagcggttg attttggtta aattttgccg
    14401 tgtgaacctg tgcaatcgaa gcagaagcta aggcagtttc atcaaagttt tcaaaccaag
    14461 tttcaagctt tccgcccaaa gcctgctcga taatttgacg agcgattttg ccgtcaaacg
    14521 gttcaacatt atcttgtagc aaggcgagct ggtcggcaag ctccggtgca aacagatcac
    14581 gtctggtagc taacatttgc ccgagcttga tccaaaccgg tccaagctct tgcaaagcca
    14641 aacgtaaacg tacaccgtaa ggtttgtctg aatgttgatt cttcacccaa aacaaagcct
    14701 tacgccctag ctttattttg cgggtaattg gcagattcgg aatggcttca tcaataccat
    14761 agcgaagaaa agtgcggata atatggtaaa agcggttgag atttttgatg ttcattgaaa
    14821 tatatattta atggtaatta ggaagatttt agcaaaattc tgtaaaaatt taaccgcttg
    14881 ttgggctact atgggtattt ctgctcaaaa ttctcaattt ctttctgtac ttccgcaata
    14941 agtgtttgtt cagaggggag gtaaagttgg tattggctgg caaaaatttt attctgattt
    15001 tccggtagcg aaaattttac caccgcatca tttttatcgg cacaaagcaa gacaccaata
    15061 gtggcatttt cgtgcggcag tttttcaact cggtcaaaat aattgacgta catctgtaat
    15121 tgccccaaat cttgatgggt gagcttgtgg gttttaattt caaaaatcac aaaacattga
    15181 agtaaacggt tgtaacaaac caaatcgaca aaaaaatcat caccgtctag gtgcaaacgc
    15241 ttttggcgtg cgataaacga gaaaccgtta cccaattcta gtagaaaatc ctgcaaatgc
    15301 gtaatgatgg cttgctctaa atccttttcg taataagcac tttcccgttt tagtccgaga
    15361 aattccaata ccatcgggtc tttaatgagc tgtttcggat caaccggctg ctgctcattt
    15421 ctggcaacag caagcacact ttccttatca ttactgagca ataatcgctc gtatagacta
    15481 ctattaattt gccgttccag ctggcgtgaa ctccaattat tcttacaact ttcagccaaa
    15541 taaaattccc ttttgtcaga atcggagatt tgaattagac gacgatattg tgtccaattc
    15601 aattgcgacc gcagtgcggt cgcaattgga aacgcacgat aaaactggcg ataacgctct
    15661 aattgacggc tacaaaaacc acttccaaat tctggctcaa gctcttttgc gagcatttta
    15721 atcaggtatg ttccataatc agcacgctct tgcccttgtt gctcctgttc aaaaatccgt
    15781 tttccgatgt gccaatacat cagcacacgt tggaaatcta ccgcacgaac cgcattttca
    15841 cgagcttgag caataatttg tttaatatct tagatcatcg ttgtttctat taacataaaa
    15901 gcctccgttt tggattaagt ttatgcttaa tctatccgga ggcaatttct ttcactcaac
    15961 ttttcgagtc atatcacaat tttattttgt taaatttaat tttgcacaaa cgcttgcgtt
    16021 tgaacatcag ctttgctgat ggctcgaaga gcgaacgtag tgagtaaatt tttgctctaa
    16081 tttgagcgct tgttgggcaa gttcttcgac ttggtcatag aaatgcaccg cctgtaaacg
    16141 atgcaccaac accggtcttt cttggattaa attctcaacc aaatgttgat tattttgctc
    16201 aaattgagct tttactttac ctagcaattt tttagcaaga tcggtgctag tttgagccac
    16261 tacatcgcca acaaaagggg aaagcagttc ggcagggtct ttttcgagct gttcaagtaa
    16321 tgcagtgaaa tgttgcaaca cttgaatatc gcccgtcagc accaacgatt tattattaat
    16381 cagctcggtt aatttttgct tatccgctag tttcggcagg gtgtcaaatg ccagttgcac
    16441 cgtacaatct gcttccccct cgtagttgcc aagccaatcg gttcggcttt cgctaaacaa
    16501 cacaaaaaag tcgattttag gcgaagttag ggcgattttt agcacttttc cggacaattt
    16561 acgcaaattc ggctcaatat gaggcgagcg tttaagtaac gcattaaaag cggtttcaat
    16621 cagaccaaag gtaaattgcg ggagcattaa ttgttgtttt acattattta acattcgtac
    16681 ctacttaccc tcttcacttt tgggagaagg atagattgtt cttcattaag aagcaaaatc
    16741 agggagaggg gtatttgcaa aatattgaat aaatatgacc gcttgccccc tctctctgct
    16801 tatttcgcta aacgctcata agcggtctct ctcccacaaa gggagagagt catttaagag
    16861 aaaactaaaa tttataacct ctgtgtaatg ccacaatgcc ggcacttaag ttgtagtaat
    16921 tcacgctttc aaagccagtt tgttccatca ttgcttttag ctcgtcttgt ttcgggtgca
    16981 ttcggatcga ttcagctaaa tagcggtaac tttcggcgtc atttaccacc acttcgccca
    17041 ctttcggcaa aatattgaat gagtagaaat tataaagctg gctgattggg tcgataatcg
    17101 gttttgagaa ttccaacaca agtaagcgac cgcccggttt tagcacgcgg aacattgaac
    17161 gtagggcttt gtctttgtcg gtaacattac gcaagccaaa actaatcaca atacaatcaa
    17221 aagtgttatc agcaaaaggc aagcattcgg cattcgcttg aacgtagctc acatttccaa
    17281 ccacgcctaa attgcgtaat ttttcccgcc cgacttccaa cattgagctg ttaatatcgg
    17341 ctaaaatcac ctcaccactt tcaccaacaa tgcgagagaa ttttgcggta aaatcacccg
    17401 tgccgccggc aagatccagc actttttgcc ctttacgcac gccactgcaa tcaatggtaa
    17461 agcgtttcca cactcgatga atgccgaaag aaagcaaatc gttcattagg tcatatttgc
    17521 ctgccaccga gtgaaacaca tttgcgacca gttgctgttt ttcttctttc gctacggttt
    17581 taaacccaaa gtgggtggtt tctgccgatt gttgcacttc ttgttgcact tcatctttag
    17641 gtgctaattg ttctacatcg ctcattttct tgtctctagt tgcaaaattt aggcgtaacc
    17701 atagcaaaaa agcggtcgtt tttctttaac tttttgcaaa ttttcttaag taaataaccg
    17761 ctttcttgac tagaaaagcc aaattctgta acattcgcac actatttttt cttgaggatt
    17821 acacattttg gacagtcata gtcgatactt acccgtttaa cctatcttgt ctaacctcaa
    17881 tgttaccgac aaggtcggta atgctcgcct agtatctttg ttcctttttg ttccaagcct
    17941 cctaactatt tggagctaaa catgatccgt gcttttggac tagataacgc atgtttaatc
    18001 agcgttgatg aaactaaccc aactttatta aatgatgcga tttggattga tctcatcgat
    18061 ccatcagaag ctgaacgaac cgtattagaa aaccgcttag accaaacact cgctgaagaa
    18121 cgtgaattgg aagacttaga ggcgtctgcc cgtttctttg aagatgaaga cggtttgcac
    18181 ctgcactcgt tcttttactg cttagatgat gacgattacg ccgatattgc cactgtcgcc
    18241 ttcactattc gtgacgggcg tttgtttacc ttgcgtgatc gggatttacc cgcatttcgc
    18301 ttgtatcgaa tgcgttcccg ccgtgagaaa ttgattgaca gcaacgccta tgaactctta
    18361 ctcgatttat ttgagaccaa aatcgagcag cttgcagacg taattgaaga agtctatgct
    18421 gacttagaaa aattaagtcg ggttattcta aacggtcagc aagaaaatga gaatttcaat
    18481 gatgcccttt ctcgcttaac tgaatttgaa gatgcaagtt caaaagtgcg tttatgtttg
    18541 atggatacgc aacgggcgtt aagtttctta ctgcgtaaaa cccgtttgcc gaataatcag
    18601 ctagaacaag cccgtgacat tatgcgagat attgaatctc tgcaaccgca caatgaatcc
    18661 ttgttccaaa aagtgaattt tttaatgcaa gcggcaatgg gttatatcaa tattgagcag
    18721 aaccgaatta tgaaattctt ctcggtggtg tcagtgatgt tcctacctgc tacgctggtt
    18781 gcttctactt acggtatgaa ctttgaattt atgccggaac ttcatttcca atatggctac
    18841 cctatggcga ttggtttaat gattgttgcc gcctccgccc cttatcttta ttttaaacgt
    18901 aaaggttggt tataatttat ttttaaag
//
LOCUS       DANLXF010000024        18896 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.024, whole genome shotgun sequence.
ACCESSION   DANLXF010000024 DANLXF010000000
VERSION     DANLXF010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 18896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18896
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.024"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            78..2513
                     /gene="plsB"
                     /locus_tag="PXD66_000502"
     CDS             78..2513
                     /gene="plsB"
                     /locus_tag="PXD66_000502"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HDL6318835.1"
                     /translation="MSGLLNFYRAFLNLPLSLLVKSRSIPTHPVAELELNLEQPIVYV
                     LPYTSQTDLLILQKNCLALNLPDPLQENVIEGQTLLRFVFLDEGRRFFKSKGAKSETE
                     SIFYRYLDLHRANAELDVQLVPVSVLWGRAPGKEDARHLQVLTSFQRFLSMVWFGRDN
                     FVRFSPAVSLRYMVTEYGADEKIAQKIARVAKMHFAKLRYSAMGPRLPNRDAMFNKIL
                     NSEVIQAAIAEEAKKSSPEKARKEAEKIINEIAADVKHESLRVADRVLSWLWNKLYQG
                     INVQNADRVRKLALEGHEIVYVPCHRSHMDYLLLSYLLYHQGLVPPHIAAGINLNFWP
                     AGPIFRSWGAFFIRRTFKGNRLYSTIFREYLAELFYRGYSVEYFIEGGRSRTGRLLEP
                     KTGMMSMSLQALQRGLNRSLSIVPVYIGYEHVLEVDTYAKELRGAAKEKENAGLVLRV
                     LKKLKNLGQGYVNFGEPILVNNYLNQYFPEWKEPSEDGRPKWLNEAVDNISHQVMVNI
                     NKAAAVNAKNLVGSALLASRQRALTREQLIEQLGSYIQLFRNVPYSKDMTLPTESAEV
                     MLNHVIHLPRSGVLIEKDNFGELVRLERESAVLMTYYRNNVQHLFVLPSLVASMVLHH
                     EAVSKDVVIKSVNRIYPFLQAELFLHFKQEELKAHIEAIIAELSRQGVIKNESDMLMI
                     NRSHIRTLQLHSAGVREILQRYYISLSVLLEQPNISRGYLEKESSSIAQRLSVLHGIN
                     APEFFDKAIFSTFTSSLKAQGYFDGDDNLAIAKVKETESLLRSLISIEVQQTIQGAMA
                     KVAESVENPNIAG"
     gene            complement(2618..3181)
                     /locus_tag="PXD66_000503"
     CDS             complement(2618..3181)
                     /locus_tag="PXD66_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HDL6318836.1"
                     /translation="MSAPNSGTNPAPLGLCGFALTTFLLSLINNGTFSGENVGLVLAM
                     GFAFGGTSQMIAGMFEFAKGNTFGFTAFTSYGAFWWSFALFKVFFAANVSAEFVGWYL
                     VAWGTFTLMMFIGTLAKARALQAIFLSLTITFYLLAAGDFTHNTTLTNIGGNFGLLTA
                     FLAFYLAAADIINESFGKTVLPIGEPK"
     gene            complement(3379..4275)
                     /locus_tag="PXD66_000504"
     CDS             complement(3379..4275)
                     /locus_tag="PXD66_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL6318837.1"
                     /translation="MDILDRLLAFAQISAGISVKCQLQGDWQLENPHQTGQAIAHIIS
                     YGQAYLQHNGKTHHLKQGDIVFFPRFADHTLRSQIQPLTSKNAVNPEQLGAFTLTQAG
                     LSQSECDMFCLHFYYDHQAELMESLPEMIVFQVAETPLKAMLDILKQEAETPNLAVKS
                     VVNSLAVVILTMILREHLVNHNNQLFGILKGWQDLHLRPLIKAILLNPEHEWQVDDMA
                     AFIHLSRAQLVRLFNKHLCISPHAFVHKIRLQKAAMLLKSQAGSVLSIALSCGFQSES
                     HFITAFKKQYCVTPSQYRQLNP"
     gene            4411..4752
                     /locus_tag="PXD66_000505"
     CDS             4411..4752
                     /locus_tag="PXD66_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDL6318838.1"
                     /translation="MSSFQNWTEHTAHVKQSFGKLGQKHPKMLEAYKALGTAAAAEAL
                     DHKTRELIALAVAITTRCESCISVHAEEAAKAGATESEIAGALATAIALNAGAAYTYA
                     LRALEAVEEQK"
     gene            4763..5116
                     /locus_tag="PXD66_000506"
     CDS             4763..5116
                     /locus_tag="PXD66_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL6318839.1"
                     /translation="MKKLAWILGFAAMMPMMSSAKQASVQQVQAEKAAGIWIDVRSAE
                     EFQQGHLNGAINVTHTEIAQRIAEVAPDKNQPIHLYCRSGHRAEAALNELKKLGYSNV
                     TNHGGYDDLIKAGLK"
     gene            5176..5814
                     /locus_tag="PXD66_000507"
     CDS             5176..5814
                     /locus_tag="PXD66_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005719747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgB family protein"
                     /protein_id="HDL6318840.1"
                     /translation="MSAFNNFVNFVANIVAPMQRQFINFVRIAICIVMVWIGGLKVCQ
                     YEADGIAHFVSNSPFFSYMYKKGPNLVDDGTGKMVMEYTLHKNPEGKMVAKNIEWHKE
                     NGTYTASYIIGATIVTVGLLTLSGIWFPVSGMAGGLLTFGMSIVTLSFMITTPEIWVP
                     NLGGDMPTPAHGFPYLSAVGRLIVKDVIMMAGGLVAAAECANRILARRKAIA"
     gene            5949..7130
                     /gene="purT"
                     /locus_tag="PXD66_000508"
     CDS             5949..7130
                     /gene="purT"
                     /locus_tag="PXD66_000508"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005615456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HDL6318841.1"
                     /translation="MTTIGTPLRTNATKVMMLGSGELGKEVVIELQRLGVEVIAVDRY
                     DNAPAQQVAHRAYTISMLDGNALRDLVEKEKPDFIVPEVEAIATATLVELEQEGYNVI
                     PTAKATQLTMNREGIRRLAAEELGLKTSPYRFVDNFEQFQQAIQEIGIPCVVKPIMSS
                     SGHGQSVIKSEADIQQAWDYSQQGGRAGGGRVIVEGFIKFDYEITQLTVRHIHGTSFL
                     APIGHIQVDGDYRESWQPQQMSDIALKKAQETAEKITSALGGRGIFGVELFVCGDEII
                     FNEVSPRPHDTGIVTMASQELSQFALHARAILGLPIPEIYRISPAASKAIVVEGKSDN
                     VRFGGVDKVLAEIGTNIRLFGKGEVNGHRRLGVILARDENTVRALEKARRAYDKLDIQ
                     L"
     gene            complement(7222..8715)
                     /locus_tag="PXD66_000509"
     CDS             complement(7222..8715)
                     /locus_tag="PXD66_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005556599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL6318842.1"
                     /translation="MFKQLQQVGKAFMLPIAILPAAGLLLGIGGALSNKATVQAYPIL
                     DNPTLQGIFQIMASAGSVVFGNLALLLCIGLGIGLAKRDKGVAALAAVVGYLIMTGTI
                     SALIPLFPPETKGIDTGVIGSLVMGLITVKLHNKYHNIQLPQILGFFGGSRFVPIVTA
                     FAAIFVGAVFFLIWPAFQQWLVSAGQGIAEMGEIGTFFYGFLMRLSGAVGLHHMIYPL
                     FWYTELGGTEVVNGETIIGAQKIFFAQLADPTHQGLFTEGTRFFAGRFDTMMFGLPAA
                     CLAMYHAVPKARRGAISGLFLGAALTSFITGITEPIEFMFLFVAPWLYVFHAFLDGVS
                     FYIADLLNISIGNTFSGGVIDFTLFGILQGNENTNWLRVIIVGLPWAALYYFSFLFLI
                     KKFNVMTPGRAEESEEVVEQQTSSLTENAHKIIAALGSANNIENVDACITRLRVAVKE
                     VKAVDKATLKKLGAIDVLEVGGGVQAVFGAKAVLYKSEVNQILGKED"
     gene            8916..10763
                     /gene="ilvD"
                     /locus_tag="PXD66_000510"
     CDS             8916..10763
                     /gene="ilvD"
                     /locus_tag="PXD66_000510"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012055207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDL6318843.1"
                     /translation="MPILRSATSTQGRNMAGARALWRATGMKENDFGKPIIAVVNSFT
                     QFVPGHVHLKDMGQLVAAEIEKAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRDLIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMAAMRLNIPTIFVSGGPMEAGKTKLSDQLI
                     RLDLVDAMIESADPNVSDERIDAIERSACPTCGSCSGMFTANSMNCLTEALGLSLPGN
                     GSMLATHADRKELFLRAGREIVELCRKYYQQDDESVLPRSIATFDAFENAMSLDIAMG
                     GSSNTVLHLLATAQEAGVDFKMEDIDRLSRVVPCLSKIAPNTNKYHMEDVHRAGGIMG
                     LLGELDRAGLIHRHVKTVLGLTIEEQLNQYDIIRNKDEDLHKFFRAGPAGIRTTKAFS
                     QDCRWDTVDDDRVNGCIRNVENAVTKEGGLAVLFGNIAEDGCIVKTAGVDESIWKFTG
                     TAIVFESQEDAVAGILGGKVKEGHIVIIRYEGPKGGPGMQEMLYPTSYLKSMGLGKKC
                     ALLTDGRFSGGTSGLSIGHASPEAASGGAIALINDGDTVAIDIPNRSINLMVSDEELA
                     ARRAEMEARGDKAWQPLKREREVSFALKVFGHFATSADKGAVRDKSLLK"
     gene            complement(10897..11247)
                     /locus_tag="PXD66_000511"
     CDS             complement(10897..11247)
                     /locus_tag="PXD66_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HDL6318844.1"
                     /translation="MWHFYLMTIELNNIQYPTDASGYLTNLNDWSVELAEKIAEKEQI
                     SLTAEHWEIIYLVRDFYQEYKTSPAIRMLVKTMAQKFGEEKGNSRYLQRLFPDGPAKQ
                     ATKIAGLPKPAKCL"
     gene            complement(11276..11941)
                     /locus_tag="PXD66_000512"
     CDS             complement(11276..11941)
                     /locus_tag="PXD66_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BAX inhibitor protein"
                     /protein_id="HDL6318845.1"
                     /translation="MENRLAHSYSTGESLISTHKVLRNTYMLLAMTLTFSAVVAFVAI
                     QMNAPAMPWWGLLVGFYGLLFLTNATSNSSAGILSVFALTGFLGYTLGPILNRYIGAG
                     LGDIVALAFGATALVFFACSAYVLTTKKDMSFISGMMVALFVVLLVGIIANIFLAIPA
                     LSLALSSLFVIFSSGAILLATSNIIHGGETNYIRATVDLYVSIYNLFVSFLQIFGVLG
                     SDD"
     gene            12147..12695
                     /gene="pgsA"
                     /locus_tag="PXD66_000513"
     CDS             12147..12695
                     /gene="pgsA"
                     /locus_tag="PXD66_000513"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDL6318846.1"
                     /translation="MKLNFPTYLTLFRVGLTPLFIVAFYLPTKYAPEISTLIFFIASI
                     TDAFDGYLARKWNQTTRLGAFLDPVADKVLVAVAFVCVAEYYHTWWITIPICIMIARE
                     IIISALREWMAELGERASVAVSIWGKVKTTAQMLALGGMLWRQNQLMEILAFILLYIA
                     VGLTIWSMMQYLNASKNSLLKS"
     gene            12773..14611
                     /gene="uvrC"
                     /locus_tag="PXD66_000514"
     CDS             12773..14611
                     /gene="uvrC"
                     /locus_tag="PXD66_000514"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDL6318847.1"
                     /translation="MSEFNAKSFLLNVPHQPGVYRMYDSKGTIIYVGKAKDLKKRLAS
                     YFRSQLSSKKTEALVANLANIETTITHSETEALLLEHNYIKENQPKYNVLLRDDKSYP
                     YILLTKHKHPRLTAFRGSKKIAGEYFGPYPNVTAVRETLNLLQKIFPIRQCEDTYYKN
                     RSRPCLQYQIGRCLAPCVEGCYSPVEYDQQVNLVRLFLQGKDHQVVEHLVQKMEDAAE
                     ALDFEGAARFRDQIQSVRAVQEKQFVSNDRLDDLDIISIVYQNGIACVHILFVRQGKV
                     LGNRSYFPKVPSNTDLTELADTFVGQFYLQMNQHRTIPNQIVIDHQLSESKALSEILS
                     EQAGHKVSILDNAIRGDKQRYLALARTNAMAALVLQLKQESHIQSRYQALKEMLKMSE
                     IKRMECFDISHTMGNQTVASCVVFDENGPLKSDYRRFNIEGITGGDDYAAMEQALLKR
                     YNRELPPEKIPDIIFIDGGKGQLNRALDVFAKLDVEWDKSKPLLIGVAKGVERKAGLE
                     TLIISKWDKEIHLPPDSLALHLIQHIRDESHQHAISGHRKKRQKAFTESGLESIAGVG
                     AKRRQALLKYLGGMQGVKSATLEEIQSVPGISKALAEVIFDTLKNS"
     gene            complement(14616..14891)
                     /locus_tag="PXD66_000515"
     CDS             complement(14616..14891)
                     /locus_tag="PXD66_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318848.1"
                     /translation="MKKLILLGLSVALTACSTPQQQSTVPMDMQAVQDYHQRVINKQT
                     VDKNTKQHDEPLNQSDKRSESINLPIHPIIYPSIGVGYHYGWKRYRY"
     gene            complement(15016..15369)
                     /gene="rplT"
                     /locus_tag="PXD66_000516"
     CDS             complement(15016..15369)
                     /gene="rplT"
                     /locus_tag="PXD66_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009635878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDL6318849.1"
                     /translation="MARVKRGVIARARHKKVLKAAKGYYGARSRVYRVAFQAVVKAAQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGLSYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFAALVEKAKSAL"
     gene            complement(15478..15675)
                     /gene="rpmI"
                     /locus_tag="PXD66_000517"
     CDS             complement(15478..15675)
                     /gene="rpmI"
                     /locus_tag="PXD66_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDL6318850.1"
                     /translation="MPKIKTVRGAAKRFKKTASGGFKRKQSHLRHILTKKTTKRKRQL
                     RHKSMVAKADQVLVVACLPYA"
     gene            complement(15939..16481)
                     /gene="infC"
                     /locus_tag="PXD66_000518"
     CDS             complement(15939..16481)
                     /gene="infC"
                     /locus_tag="PXD66_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015871208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDL6318851.1"
                     /translation="MKPVKRVQSNRAHRLNDEIRGVKEVRLTDENGEQVGIVSLQDAL
                     NRAEEAELDLVEISPNAEPPVCRIMNYGKFIYEKEKAAKEQKKKQKVVQVKEIKFRPG
                     TDDGDYQVKLRSIIRFLEDGDKVKITVRFRGREMAHQEIGMDVLERVKADTAEIAMVE
                     SAPGKLEGRQVVMVIAPKKK"
     gene            complement(16640..18439)
                     /gene="recQ"
                     /locus_tag="PXD66_000519"
     CDS             complement(16640..18439)
                     /gene="recQ"
                     /locus_tag="PXD66_000519"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDL6318852.1"
                     /translation="MNTTAISVLNNIFGYQKFRDGQEEVINAVLENRDCLVIMTTGGG
                     KSLCYQVPALCLDGITLVISPLISLMKDQVDQLITNGIEAAYLNSTQTLEEQQLVEQK
                     ALSGQLKLLYLSPEKVMTQGFFHFISLCKISFIAVDEAHCVSQWGHDFRPEYTLLGGL
                     RNTFPNVPLMALTATADLTTRSDILHHLRLNSPHIYLGSFDRPNIRYTVQEKFKPLEQ
                     LIKFISKQQGKSGIVYCNSRKKVEEITEKLSARKISVMGYHAGMSFEQRERVQNAFQR
                     DDIQIVVATIAFGMGINKSNVRFVVHFDLPRSIESYYQETGRAGRDDLPSEAVLFYDP
                     SDYAWLQKILLEEPESEQRNIKQHKLQAIGDFAESQTCRRLVLLNYFGEHRQEQCKNC
                     DICLDPPQQYDGLLDAQKVMSVIYRTGQIFGQHHIIAVLRGMNNLKIREHQHDQLSVY
                     GIGKEQSQHYWQNVVRQLIHLGLIIQNIANHSALQLTESARPFLRGEKQLQLALPRLG
                     ISATAFVQKQASHRYDKDLFARLRFLRKQIADLENIPPYIVFNDATLQEMAEFLPTSK
                     VEMLEINGVGERKFERFGQAFMSLIKEYQNNRK"
     gene            complement(18548..18853)
                     /gene="cyaY"
                     /locus_tag="PXD66_000520"
     CDS             complement(18548..18853)
                     /gene="cyaY"
                     /locus_tag="PXD66_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HDL6318853.1"
                     /translation="MNTAQFHQAVEQVWQQIEEKIYDEGLSVDTEIQGAVCTLTFDDE
                     SQVIINKQEAMLELWLASKLGGFHFKNVEGEWVTDEGRSFWQHLEEAFARHGESVSF"
BASE COUNT         5495 a         3604 c         4140 g         5657 t
ORIGIN      
        1 tccataaaaa tttatggtag aatggaagaa attagaggtt agacctccaa caataacgat
       61 tctaaaagga tatttatatg tctggcttat taaattttta tcgagctttc ctaaacttac
      121 cactttcact tttagtaaaa tctcgctcta ttccgactca tccggtggct gaactggaac
      181 taaatcttga acagccgatt gtctatgttc taccttatac ctcgcaaacc gatttattaa
      241 ttttacaaaa aaactgcttg gctttgaatt tgcccgatcc gttgcaagaa aatgtgattg
      301 aagggcaaac attgcttcgt tttgtatttt tagatgaagg tcgccgtttt tttaaatcta
      361 aaggggcgaa aagtgagaca gagtcgattt tttatcgtta tttagattta catcgtgcta
      421 atgctgaatt agacgtccaa ttagtgccgg tttcggtatt atgggggcgg gctccgggca
      481 aagaagatgc ccgccattta caggtgctta cctcattcca acgtttttta tccatggttt
      541 ggtttgggcg tgataatttt gtacgtttct cccctgcagt atcgttacgc tatatggtaa
      601 ctgaatatgg tgcagatgaa aaaatcgctc aaaaaatcgc gcgtgtggcg aaaatgcact
      661 ttgcaaaatt gcgttattca gcaatggggc ctcgtttacc aaatcgtgat gcgatgttta
      721 ataaaatcct gaattcagag gtgattcaag ctgcgattgc ggaagaagcg aagaaatcat
      781 cgcctgaaaa ggctcgcaaa gaagccgaga aaatcattaa tgaaattgcc gctgatgtaa
      841 aacacgagag tttgcgtgta gcagatcgag ttctgagctg gctttggaat aagctttatc
      901 aagggatcaa cgtgcaaaat gcagaccgtg tgcgtaaatt ggcattagaa ggtcacgaga
      961 tcgtttatgt gccttgccac cgtagccata tggactattt gctcctatct tatctacttt
     1021 accatcaagg attagtaccg ccacatattg ccgctgggat caatttaaat ttctggccgg
     1081 caggcccgat tttccgtagt tggggagcat ttttcattcg ccgtaccttt aaaggaaatc
     1141 gcctctattc caccattttc cgtgaatatt tagcggaatt attctatcgt ggttattcgg
     1201 tcgaatattt tattgaaggc gggcgttcac gcacaggtcg tttattagag cctaaaaccg
     1261 gtatgatgtc gatgagccta caagccttac agcgagggct gaatagatct ttaagtattg
     1321 tgcctgttta tatcggttat gaacacgtct tagaagtcga tacttacgct aaagagttga
     1381 gaggtgcggc aaaagagaaa gaaaatgcgg gcttggtgct acgagtgctg aaaaaactga
     1441 aaaatttagg tcaaggatat gtgaatttcg gtgagccaat tttggtgaat aattacttaa
     1501 atcaatattt ccctgagtgg aaagagcctt ccgaagatgg acgacctaag tggttaaatg
     1561 aagccgtgga taatatttca catcaagtaa tggtgaatat caataaagcg gcagcagtta
     1621 atgcgaaaaa cttagtgggg tcagcattat tggcatctcg ccagcgtgca ttaacccgtg
     1681 aacaactcat tgaacaactt ggaagttata ttcagttatt tcgtaatgta ccttattcta
     1741 aagatatgac attaccaact gaaagtgcag aagtgatgtt gaatcatgtt attcatttac
     1801 ctcgttcggg cgttttaatt gaaaaagata attttggtga actggttcgt ttagaacgag
     1861 aatctgctgt gttaatgaca tattacagaa ataatgtgca gcatttattt gtgttgcctt
     1921 cacttgttgc aagtatggta cttcatcatg aggctgtttc gaaagatgtt gtgattaagt
     1981 cggttaatcg tatttatcca ttcttgcaag cagagttatt cttacacttt aagcaagaag
     2041 aattaaaagc acatattgaa gcaattattg cggaactaag tcggcagggg gtgattaaaa
     2101 acgaaagtga tatgttaatg atcaatcgct cacatattcg taccttgcaa ttacattcag
     2161 ctggtgtgcg tgaaatcctt caacgttatt atattagcct aagtgtcttg ttggagcagc
     2221 caaatattag tcgtggctat ttagaaaaag agagtagttc aattgctcag cgtttatcag
     2281 tcttgcatgg tattaatgcg ccggagtttt ttgataaggc gattttctct acctttacat
     2341 caagtctaaa agcacaaggg tattttgatg gtgatgataa tttagcgatt gcaaaagtta
     2401 aagaaacgga atcgttgtta cgtagtttga tttcgattga agttcaacag accattcaag
     2461 gtgcgatggc taaagtcgca gaatctgtag agaatcctaa tatcgcagga taataaatca
     2521 aagaagggcg ctaacaagcg ctcgtttctt gcaattttgt tgccaaaaat aaaagctgaa
     2581 tgcgcgacca cattcagctt ttttacttcc tatataatta ttttggttca ccgattggta
     2641 aaacagtctt accaaaactt tcattaataa tatccgcagc ggctaagtag aacgctaaga
     2701 atgcggttaa taaaccgaag ttaccaccaa tgttagttag ggtggtgtta tgggtaaaat
     2761 cacctgcggc taaaaggtag aaagtaatgg ttaaacttaa gaaaatagct tgtaatgcac
     2821 gggcttttgc tagtgtgccg atgaacatca taagtgtgaa tgttccccaa gctactaagt
     2881 accaaccaac gaattctgcc gatacattcg cagcaaagaa cactttaaat aatgcaaaag
     2941 accaccagaa tgcaccatag ctggtaaacg cagtaaagcc gaacgtgtta cctttagcaa
     3001 attcaaacat acctgcaatc atttgtgatg tgccaccgaa agcaaatccc attgctaaaa
     3061 ctaaacctac gttttcaccg ctgaatgtac cgttattgat taggcttagt aaaaatgtag
     3121 taagtgcaaa accgcataaa ccgagcggag ccgggttagt tcctgaattt ggagctgaca
     3181 ttgaaaacct ctgttatatt gttacaccat aagtgtggta attgtttatt tacgccgata
     3241 aatcagacgt tatgtgccta aaatttaggg cgataaagta tagcgatttt gcagtaaatt
     3301 gactagaaat ttccgagaca agcggtgaga tttttgcaaa attttgccaa aaaatgaccg
     3361 cttgttgttg tcatttagct atggatttaa ctgccgatat tggctgggtg ttacgcaata
     3421 ttgcttttta aaggcggtaa taaaatgcga ctcggattgg aagccgcaag ataaggcgat
     3481 acttaatacc gacccagctt ggcttttgag cagcattgct gctttttgta gcctaatttt
     3541 gtgtacaaaa gcatgtgggc taatacataa atgtttgtta aataagcgga ctaactgggc
     3601 acgagaaagg tggataaatg ccgccatatc atcgacttgc cattcgtgtt cgggattaag
     3661 caaaatagct ttgatcaaag gtcttaagtg taagtcttgc caacctttta aaataccgaa
     3721 taactgattg ttatggttaa ctagatgttc tcgtaagatc atagttaaaa taacaacagc
     3781 aagcgagtta accacagatt ttacggctaa attaggtgtt tctgcttctt gttttagaat
     3841 atctaacatt gcttttaatg gtgtctcggc aacttgaaat acgatcattt caggtaagct
     3901 ttccattaat tcggcttgat ggtcataata aaaatggagg cagaacatat cgcattctga
     3961 ttgagaaagt cctgcttggg tgagtgtaaa tgcacccaat tgttcagggt ttaccgcatt
     4021 tttgctggtc aaaggctgta tttgactacg taatgtatga tcggcaaatc tggggaaaaa
     4081 tacaatatct ccctgtttaa ggtgatgggt tttcccatta tgttgtagat aagcttgccc
     4141 atagctgatg atatgagcga ttgcttgtcc ggtttgatgc ggattctcaa gttgccaatc
     4201 tccctgtaac tgacatttca cactgattcc ggctgagatt tgagcgaaag ctagaagcct
     4261 atctaatata tccattgttc attccttata tttccacctt aaattgcgat tatttcatta
     4321 taatatgagc tgatttgata ataaagttta tcagataaac gcaataataa atgcgttttt
     4381 tcatattatt cactttagga ggaaatcaat atgtcttcat tccaaaattg gacagaacac
     4441 acagctcacg ttaagcaatc ttttggtaaa ttagggcaaa aacaccctaa aatgcttgaa
     4501 gcttataaag cattaggtac agcggccgca gcggaagcat tagatcacaa aactcgtgaa
     4561 ttaattgcat tagcagtagc gattacaaca cgttgtgaaa gttgcatcag tgtacacgca
     4621 gaagaagcgg caaaagcagg tgcaaccgaa agcgaaattg ccggtgcatt agcgactgcg
     4681 attgcattaa atgccggtgc ggcatatacc tatgctttac gtgcattaga agcggtggaa
     4741 gagcaaaaat aaggagcaat aaatgaagaa attggcttgg attttaggct ttgcagctat
     4801 gatgccaatg atgtctagtg ctaaacaagc atcagttcag caagtgcagg ctgaaaaagc
     4861 agcgggcatt tggattgatg tacgttctgc tgaagaattt cagcaagggc atttaaatgg
     4921 tgcaattaat gtaacgcata ctgaaattgc acaacgtatc gcagaagttg ctccagataa
     4981 aaatcagccg attcatcttt attgccgctc gggtcatcga gctgaagcgg cgcttaatga
     5041 gctaaaaaaa ttaggttaca gcaatgtaac taatcatggt ggttatgacg atctgattaa
     5101 agccgggttg aaataactgt tttgagtgcc aagcatttgc ttggcacttt tcgtttatat
     5161 ctgaggttaa aaattatgag tgcttttaac aattttgtta attttgttgc aaatattgtt
     5221 gctccaatgc aacgccaatt cattaacttt gtacgtattg ccatttgtat cgtgatggtt
     5281 tggattggtg gtttaaaagt ctgccaatat gaagcagacg gtattgccca ttttgtgtct
     5341 aatagcccgt tctttagtta tatgtacaaa aaaggcccga atttagttga tgacggcaca
     5401 ggtaaaatgg tgatggaata tacccttcac aaaaaccctg aaggtaaaat ggtggcaaaa
     5461 aacatcgaat ggcataaaga aaacggcact tatactgcat cttatatcat cggtgcaacc
     5521 attgtgacgg ttggtttatt gacgttatcc ggtatttggt tcccggtttc cggtatggcg
     5581 gggggcttac tcacctttgg tatgtcgatt gttacactct cttttatgat caccacccct
     5641 gaaatttggg tgccgaattt aggtggggat atgccaaccc cagcgcatgg tttcccatat
     5701 ctgtcagcag tcggtcgttt aattgtgaaa gatgtgatta tgatggcagg cggcttggtt
     5761 gccgcagcgg aatgtgcaaa tcgcatttta gccagacgta aagcgattgc ttaattgata
     5821 gtggtaatgc aagcggtcat tttcttgtaa aattttgcaa taaaatgacc gcttgttttt
     5881 tgtcaatttg ccataatttc aagtataatt caattacgta atcgtttgct tttatggaga
     5941 ggaataaaat gacaacaatc ggtacaccgt taagaacgaa cgctacaaaa gtgatgatgt
     6001 taggttcggg cgagttgggg aaagaagtgg taatagagct gcaacgctta ggcgtagaag
     6061 tgattgcggt ggatcgttat gacaatgcac ctgcacagca agtggctcac cgtgcttata
     6121 cgatttcgat gttagacggg aatgcattgc gagatttggt ggagaaagaa aaaccggatt
     6181 ttattgtgcc cgaagtagaa gccatcgcaa ctgcaacctt ggttgagtta gagcaagagg
     6241 gatataacgt aatcccaacg gcaaaagcaa cgcaattaac aatgaatcga gaaggcattc
     6301 gccgattggc ggcagaagag ttgggcttaa aaacgtcgcc ttatcgtttt gtggataatt
     6361 ttgaacagtt ccagcaagcg attcaagaaa tcggcattcc gtgtgtggtc aaaccgatta
     6421 tgtcttcttc cggacacggg caatcagtaa ttaaatctga agcggatatt caacaggctt
     6481 gggattattc gcaacagggc ggacgtgccg gcggtgggcg tgtgattgta gagggtttta
     6541 tcaaattcga ttatgaaatt acccagctta ccgttcgcca tattcacggc acgtcgtttt
     6601 tagctcccat cgggcatatt caggttgatg gcgattatcg tgaatcttgg caaccgcagc
     6661 aaatgtcgga cattgcattg aaaaaagctc aagaaaccgc agaaaaaatt acctctgcac
     6721 tgggcggaag agggattttc ggcgtggaac tttttgtctg tggcgatgag attattttta
     6781 acgaagtctc cccacgtcca cacgataccg gaatagtaac aatggcatca caagagcttt
     6841 ctcaatttgc tctgcacgcc cgtgcgattt tgggcttacc gattccggaa atttaccgca
     6901 tcagcccggc agcttctaaa gcgattgtgg ttgaagggaa atcggataat gtgcgttttg
     6961 gcggtgtaga taaggtatta gccgaaatcg ggacgaatat ccgcttattc ggtaaaggcg
     7021 aagtaaacgg acaccgccga ttaggggtaa ttcttgctcg tgatgaaaat acagtgagag
     7081 cattagaaaa agctcggcga gcttatgata agttagatat tcaattataa agtcaggatt
     7141 ttgatattcg ttgatagtag gcattttatg cctactatcc tataggcctc taatcaaatt
     7201 ggatgaaaaa gttgaagatt gttaatcctc tttgccgaga atttggttca cttcgctctt
     7261 atacaatact gcttttgctc caaatacggc ttgtacaccg ccaccgactt ccagcacgtc
     7321 aatcgcaccg agttttttca gtgttgcttt atccacggct ttcacctctt tcactgccac
     7381 acgcagacgg gtaatacaag catccacatt ttcgatatta tttgcagagc caagagcggc
     7441 gataatttta tgggcatttt cagtcagtga gctggtttgt tgttcgacta cctcttcact
     7501 ctcttccgca cgtcccggtg tcattacgtt gaattttttg atcaggaata ggaacgagaa
     7561 gtagtaaagt gccgcccacg gcaagccgac tatgatcact cgaagccagt tagtgttctc
     7621 gttgccttgc aaaatgccaa acagggtgaa gtcgatcacg ccaccggaga aggtgttacc
     7681 aatcgagatg ttgagtaagt ctgcaatgta gaacgacacg ccgtctaaaa aagcgtggaa
     7741 cacatagagc cacggggcaa caaacaggaa cataaattca atcggctcgg taatgccggt
     7801 gataaaagag gtgagcgctg cacctaagaa cagaccgcta atcgcaccac ggcgggcttt
     7861 cggcacagcg tgatacatcg ctaaacaggc ggcaggtaag ccaaacatca tcgtatcgaa
     7921 acgtccagcg aagaaacgtg taccttcggt aaataagcct tgatgggttg gatcggcgag
     7981 ttgggcgaag aagatttttt gcgccccgat aatggtttca ccatttacca cttctgttcc
     8041 gcccaattcg gtgtaccaaa acagcgggta gatcatatgg tgtaagccga ccgcaccgct
     8101 taaccgcatt aagaaaccgt agaaaaaggt gccgatttcg cccatttccg caatcccttg
     8161 ccctgctgaa accaaccact gttggaaagc cggccagatc aaaaagaaca ctgccccgac
     8221 aaaaatcgca gcaaaggcgg tcacaatcgg cacaaaccga gagccgccaa agaagccgag
     8281 aatttgcggc agctgaatgt tgtggtattt gttatgcagt ttcaccgtaa tcaagcccat
     8341 taccagcgag ccgatcacac cggtatcaat ccctttggtt tcaggaggaa acagcggaat
     8401 gagggcagaa atcgtgcctg tcataatcaa ataaccgacc actgctgcca gtgccgctac
     8461 gcctttatct cgttttgcta atccaatgcc taaaccgata cacagtaata atgcaaggtt
     8521 gccaaacacc acactgcctg ccgatgccat aatctggaaa atgccctgta aagtcgggtt
     8581 atccagtatc gggtaggctt gcaccgttgc cttattcgac aaagctccgc caatccctaa
     8641 cagcaaaccg gctgccggta aaatcgcaat cggcagcata aaagcttttc cgacttgttg
     8701 cagttgtttg aacataaaat ttctcctttt ttatggttgt atttgatggt ttatagcata
     8761 ctgaaattta gccgtatgag gtgtgatata actcacagat ttgcaaaatt tttagcaaat
     8821 tcgaccgctt gtaaaaaaat taccgcttgc ggaaaaattg aagtagaatt aagcattttt
     8881 ttagcaaaca caacataaaa aatttggaga agattatgcc tattttacgt tcagcaactt
     8941 ccactcaggg tcgtaatatg gcgggggctc gtgcattatg gcgtgcaacc ggtatgaaag
     9001 aaaatgattt tggtaagccg attattgcag tggtgaactc attcacccaa tttgtgccgg
     9061 ggcacgtcca cttaaaagat atggggcagt tggttgcggc agaaattgaa aaagccggtg
     9121 gggtggcaaa agaattcaac accattgcgg ttgatgacgg cattgcaatg ggacacggcg
     9181 gaatgcttta ttctttacca agccgtgatt taattgcaga cagtgtggaa tatatggtga
     9241 atgctcactg tgctgatgcg atggtgtgta tctctaactg cgataaaatc accccgggaa
     9301 tgttaatggc agcaatgcgg ttaaatattc cgactatttt tgtctctggt ggcccgatgg
     9361 aagccggaaa aactaaactt tccgatcaat taattcgctt ggatttagtc gatgcaatga
     9421 tcgaaagtgc cgacccgaat gtgtctgatg aacgtattga tgcgattgaa cgtagtgctt
     9481 gcccgacttg tggttcttgt tcaggtatgt tcaccgcaaa ctcaatgaac tgtttaactg
     9541 aagctttagg cttaagttta ccgggcaatg gttcaatgtt ggcaactcat gctgaccgta
     9601 aagagctttt cctaagagca ggtcgtgaaa ttgtggaact ttgccgtaaa tattaccaac
     9661 aagacgatga gagcgtgtta cctcgctcaa tcgctacctt cgatgcattt gaaaatgcaa
     9721 tgagccttga tattgcaatg ggtggttcaa gtaataccgt gcttcattta ctagcgactg
     9781 ctcaagaagc cggcgttgat tttaaaatgg aagatattga ccgcctctct cgtgtcgtgc
     9841 cgtgcttaag taaaattgcc ccgaatacca ataaatacca tatggaagac gtacaccgtg
     9901 ccggtgggat tatgggcttg ctgggcgaat tagatcgtgc aggtttaatc catcgccacg
     9961 ttaaaacagt gctaggttta acgatagaag agcaactgaa tcaatacgat attattcgta
    10021 ataaagatga agatttacac aaattcttcc gtgcaggtcc ggcaggaatt cgtactacta
    10081 aggctttctc gcaagattgc cgttgggaca ctgttgatga tgaccgtgta aacggctgta
    10141 ttcgcaatgt ggaaaatgct gtgaccaaag agggcgggtt agcggtatta tttgggaata
    10201 ttgcagaaga tggatgtatt gttaaaaccg caggggtaga tgagtctatt tggaaattca
    10261 ctggcaccgc aatcgtgttt gaaagccaag aagatgcggt agcaggtatt ttaggcggaa
    10321 aagtgaaaga agggcatatt gtgattatcc gctatgaagg tccgaaaggc ggcccgggta
    10381 tgcaagaaat gctttatcca actagttatt tgaaatcaat gggcttaggc aaaaaatgtg
    10441 ctttattaac tgacggtcgt ttctctggcg gtacctcagg gttatctatc ggacacgctt
    10501 caccggaagc cgcttcagga ggagcgattg ccttaattaa tgatggcgat acggtggcga
    10561 ttgatattcc gaatcgttcg attaacttaa tggtttcaga tgaagaactt gctgctcgcc
    10621 gtgcagaaat ggaagcccgt ggcgataaag cgtggcagcc gctaaaacgt gagcgagaag
    10681 tgtcgtttgc actaaaagtg tttggacatt ttgccacctc tgctgataaa ggtgcggtga
    10741 gggataaatc gctgttaaag taaataaatt agaaaaaaga agcatttcaa taatatattg
    10801 atctgctctt tattcagctg aatatttaat ctagctgaat ggagagcaga tcgatagcct
    10861 aatttacatc tttttgtttt gatacaggga aatgtattac aagcattttg ccggttttgg
    10921 cagacctgca attttagtgg cttgttttgc aggaccatca ggaaacaagc gttgtaaata
    10981 acggctgttg cctttttctt caccgaattt ttgtgccatt gtctttacca gcattcgaat
    11041 tgctggtgaa gttttgtatt cttggtagaa atcccgcacc agataaataa tttcccaatg
    11101 ttctgcggtt aagctaattt gctctttttc ggcgattttt tctgctaatt ctacagacca
    11161 atcatttaaa ttagttaaat agcctgaagc atcggttggg tattggatat tatttaattc
    11221 aatcgtcatc aaataaaaat gccacataat gtggcattcc tcttaaacta aaacgctagt
    11281 cgtcactacc tagcacaccg aagatttgta agaagcttac aaataggttg tagatagaca
    11341 catataaatc aacggttgca cgaatgtagt tggtttcgcc accgtgaata atgttgctgg
    11401 ttgccagtaa aatcgcaccg cttgagaaaa tgacaaacaa gctgcttagg gctaaactta
    11461 atgccggaat tgctaagaag atattggcaa taataccgac taataatacc acaaacaggg
    11521 caaccatcat tccgctgata aagctcatat cttttttggt ggttaataca taagctgaac
    11581 aagcgaagaa aaccaatgcg gttgcaccga atgcaagggc gacaatatcc cctaaacccg
    11641 caccgatgta acggtttaaa ataggaccta aggtatagcc taagaagccg gttaaagcaa
    11701 atacgcttaa gatacccgca ctgctgtttg aggtggcatt cgtcaagaaa agtaagccgt
    11761 aaaagccgac caataatccc caccacggca tagccggtgc gttcatttga attgcaacaa
    11821 aagcaacaac tgcggagaaa gtcagggtca ttgccaataa catataggta ttgcgaagca
    11881 ctttgtgggt gctaattaga gattcaccag ttgaatagct gtgagctaaa cgattttcca
    11941 taaaaattcc ttctgttttt gtgtttaaat cggaacatcc gatggattaa tatctatgga
    12001 taaatttcca taaatcaaga agtaaggtag ccgtaattta ttgtaaagtc aatgcgattt
    12061 tgtaacagaa gagtaaaaaa aaacatttag ttgtgttaca cttaatacca ctctacttta
    12121 tattcctttt catcgatgaa ttcgctatga agctcaattt cccaacttat ttaaccttat
    12181 ttcgggtagg attaacccca ctttttattg tggcttttta tctgccgaca aaatatgcgc
    12241 ctgaaatttc aacacttatt ttttttattg catcgattac cgatgccttt gacggctatt
    12301 tagcacgcaa atggaatcaa accacccgac ttggtgcatt tttagatcct gttgcggata
    12361 aagtcttggt agctgttgcg tttgtctgcg tggcggagta ttaccatact tggtggatta
    12421 ctattccaat ctgcattatg attgcccgtg aaattattat ttcggctttg cgtgaatgga
    12481 tggcggagct tggggaaagg gccagtgttg ccgtttcgat ttgggggaaa gtaaaaacga
    12541 ctgctcaaat gctcgcatta ggtggaatgt tatggagaca gaaccaacta atggagattt
    12601 tagcctttat tctgctttat attgcagtgg gtttaacgat ctggtctatg atgcagtatt
    12661 taaatgcttc aaagaatagt ctattgaagt cataagcggt catattttta ttcttttttg
    12721 caatttgtag agtggaaata tcgctctaca attttcaatt ttatcaactc taatgtctga
    12781 atttaacgct aaatccttcc tgttaaatgt gcctcaccaa ccgggtgttt atcgtatgta
    12841 tgatagtaaa ggcacgataa tctatgtggg taaagccaaa gatttaaaaa agcgtttagc
    12901 cagctatttt cgtagccaac tttccagtaa aaaaacggaa gccttggtgg caaatcttgc
    12961 taatattgaa acgacgatca ctcattctga aacagaggcg ttactccttg aacataatta
    13021 tattaaggaa aatcagccga aatataatgt gttgctcaga gatgacaaat cttatcctta
    13081 tattctgtta actaaacata agcatcctcg tttaacagca tttcgtggta gtaaaaagat
    13141 cgccggtgaa tattttgggc cttatccgaa tgttacggca gttcgggaaa cactgaattt
    13201 attgcaaaaa atttttccta ttcgtcagtg tgaagatact tattataaaa accgttcacg
    13261 accttgctta cagtatcaga tcggacgctg tttagcccct tgtgtagagg gctgttattc
    13321 acccgttgaa tacgatcagc aggttaattt ggttcgtttg tttttacagg gaaaagatca
    13381 ccaagtagtc gagcatctgg tacagaaaat ggaagatgcg gcggaggctt tggattttga
    13441 aggtgcggca cgttttcggg atcaaattca atcagtaaga gcggtgcaag aaaaacaatt
    13501 cgtctctaat gataggctag atgatcttga tattatctcg atcgtttatc aaaatgggat
    13561 tgcctgtgtg catatcttat ttgttaggca aggtaaggta ctaggcaatc gttcttactt
    13621 tccaaaagtg ccaagtaata cagatttaac cgagctcgca gatacctttg tcggtcagtt
    13681 ttatttacaa atgaaccagc accgcaccat acctaaccaa attgtaattg atcatcaatt
    13741 aagtgaatct aaggcactca gtgaaatttt aagtgagcaa gcagggcata aagtcagcat
    13801 tttagataat gccatcagag gtgataaaca gcgttattta gctttagcga gaactaatgc
    13861 aatggcagca ttagttttac aacttaagca agaatcccat attcaaagcc gttatcaggc
    13921 attgaaagaa atgttgaaaa tgtcagagat taaaagaatg gagtgctttg acatcagtca
    13981 tacaatgggc aaccaaacag tggcttcttg cgtggtgttt gatgaaaatg ggccgctaaa
    14041 atcggattac cgccgtttta acattgaggg gattaccgga ggtgatgatt atgcagcaat
    14101 ggagcaggct ctcttgaaac gctataatcg agaattaccg ccagaaaaga tccctgatat
    14161 tatttttatt gatggtggca aagggcagtt gaaccgagcg ttagatgtct tcgctaaact
    14221 tgatgtggaa tgggataaat cgaaaccgct tttaattggt gtggcaaaag gcgtagaacg
    14281 taaagctggg ctggaaactt taattatcag caaatgggat aaagaaattc acttaccgcc
    14341 agatagtctt gccttgcatt taattcagca tattcgagat gaatcccacc agcatgccat
    14401 tagtgggcat cgtaaaaaac gccaaaaggc ctttaccgaa agtggattag aatctattgc
    14461 cggtgtcggt gcaaaacgcc gacaagcgtt gctaaaatat ttaggcggaa tgcagggggt
    14521 gaagtctgca accttggaag agattcaatc agttccggga atttctaaag cgttggcaga
    14581 ggttattttt gatacattaa aaaatagtta aattatcaat agcgatatcg cttccagccg
    14641 taatggtagc ccactcctat gcttgggtaa attatagggt gaattggcag atttatgctt
    14701 tctgagcgtt tatcgctttg gtttaatggc tcatcatgtt gctttgtatt tttatcaaca
    14761 gtttgcttat tgattactcg ttgatggtaa tcctgaactg cttgcatatc cattggtact
    14821 gtgctttgtt gttggggtgt cgaacaggcg gttagtgcaa cgctcaatcc gagtaaaatt
    14881 aattttttca tatcatttcc ttatattgca aaatattttg cttttttgac cgcttatttt
    14941 gctattaatt cttaaaaaga ttattttatc cataaaaaag gacgcactag gcgtcctcat
    15001 ttctattctt taaaattaaa gagcagattt tgctttttca actaaagctg cgaaagcaac
    15061 tttgtcaaat actgcgatgt cagctaagat cttacggtcg atttcaacag atgctttttt
    15121 caagccgttg atgaatttgc tgtaagataa accgttttga cgagctgctg cgttaatacg
    15181 agcaatccat aattgacgga attgacgttt acgttgacgg cggtcacggt aagcatattg
    15241 agcagcttta actactgctt ggaaagcaac gcgatacacg cgtgaacgtg caccataata
    15301 acctttagca gccttaagaa ctttcttatg gcgtgctctt gcaataacac cacgttttac
    15361 acgagccatt atttaatctc ctaattgtct attttgattg gtggtcgtca tcacgacctt
    15421 tactaagaat tctatccgta cgtccgtact ttactacact acgctaacct aaaaggctta
    15481 tgcgtatggt aagcacgcta ctactaaaac ttggtcagct ttcgctacca ttgatttgtg
    15541 acgtaattga cgtttacgtt tagtggtttt cttagtcaaa atgtgacgta agtgagattg
    15601 tttacgttta aaaccgccgg aagctgtttt tttgaaacgc ttagcagcac cacgtactgt
    15661 tttaatttta ggcattgtta aaaaactccg cattgtttgt tagatattat gaaaataagg
    15721 cgaattttgt gaacaaaatt acttggaagc acttattctc cgctatacga ttttcatcgt
    15781 taaaaagtca agcaggctgc tgagtttgcc tgttgagcat tttctctttc ttggctaggc
    15841 aaaaaagctt gcgaatttta gcgaaaatat aaccgctatg caagccaatt taagctttat
    15901 caagaaaaaa gcacattaaa acaatgtgct tatcgaaatt atttcttctt aggtgcgatc
    15961 accatcacaa cttggcgacc ttccaatttg cctggggctg actctaccat tgcaatttct
    16021 gcagtatctg ctttgacacg ttctaaaacg tccataccga tttcttggtg agccatttca
    16081 cgaccacgga aacgcactgt gatttttact ttatccccat cttctaagaa acggataatg
    16141 ctacgaagtt taacttgata atcgccatca tcagttcccg gacggaattt gatctcttta
    16201 acttgaacaa ctttttgttt tttcttttgt tcttttgctg ctttctcttt ttcgtagatg
    16261 aacttaccat agttcataat acgacaaact ggtggttcag catttgggct gatttccact
    16321 aaatccaatt cagcttcttc tgctctattt agagcatctt gaagtgatac aataccaact
    16381 tgttcgccgt tttcatcggt taagcgaact tcttttacgc cacgaatttc gtcattaaga
    16441 cgatgtgcac gatttgattg aacacgtttt acaggtttaa tagccttatt ccttctagta
    16501 aatttttgct taaattttac cgcttgagag cgtataaata ttttagattg cttgttttaa
    16561 gtgattaaaa aacagctaaa cgtgcggatt ctataagatt ttcagtgctt atgcaataat
    16621 aaagccctta tttaactgcc tattttcggt tattttgata ttctttgatt aaactcataa
    16681 atgcttgccc aaatcgttca aattttcgtt caccaacacc attaatttct aacatttcca
    16741 cttttgaagt cgggagaaat tctgccattt cttgcaaggt ggcatcgtta aagacgatat
    16801 aaggtggaat attttcgagg tcagctatct gcttgcgtaa aaaacgtaag cgtgcgaata
    16861 ggtctttgtc atagcgatga cttgcttgtt tttgcacaaa tgcagtcgca gatataccta
    16921 aacgtggcaa tgcaagctga agttgctttt ctccacgcaa aaatggtctg gcactttcgg
    16981 ttaattgcag agcggaatgg ttagcaatat tttgaataat caaccctaaa tgaatcagtt
    17041 gccgcaccac attttgccaa taatgttggc tttgttcttt tccgatacca taaacggaaa
    17101 gttgatcgtg ttgatgttca cgaattttga gattgttcat tcctcgcaat accgcaataa
    17161 tatggtgctg accgaaaatt tgaccggtgc ggtaaatcac cgacatcact ttttgggcat
    17221 caagtaagcc atcgtattgt tgcggtggat ctaaacaaat atcgcagttt ttgcattgct
    17281 cttggcgatg ttccccaaag tagttgagta agactaaacg gcggcaagtc tgtgattcgg
    17341 cgaaatcgcc aattgcctgt aatttatgct gtttaatatt tctctgttcg ctttccggct
    17401 cttccaataa tattttttgt aaccaagcat aatcgctagg atcataaaaa agcacggctt
    17461 cagaagggag atcatctcgc ccggctcgtc cggtttcttg atagtaagat tcgatagagc
    17521 gaggtaaatc gaaatgtacc acaaaacgta cgttggattt gttgatcccc ataccaaacg
    17581 caatagtggc aaccacaatt tgaatatcat cacgctgaaa ggcattctgc actcgttctc
    17641 tctgttcaaa actcataccg gcgtgatagc ccatcacgct gattttacgg gctgagagtt
    17701 tctcggtgat ttcttccact tttttgcgac tgttgcagta aacaataccg cttttgcctt
    17761 gctgtttgct gatgaattta atgagctgtt caagcggttt aaatttttcc tgcacggtgt
    17821 agcgaatatt ggggcgatca aagctgccta aatagatatg tgggctattt aagcgtaaat
    17881 gatgcagaat atcggagcgg gttgttagat ctgccgtggc ggtgagtgcc atcaacggca
    17941 cattcgggaa agtgtttcgt aagccaccta gcagggtata ttcagggcgg aaatcgtgcc
    18001 cccattggga aacacaatgg gcttcatcaa ccgcaataaa gctgatttta catagcgaaa
    18061 taaagtggaa aaagccttga gtcattacct tttcagggga aagatagagt aattttaatt
    18121 gaccggataa ggctttttgt tccacaagtt gctgctcttc gagcgtttga gtggagttaa
    18181 gataagctgc ttcaatgccg ttggtgataa gttgatccac ctggtctttc atcaacgaaa
    18241 taagcggcga aatcaccaaa gtgatgccgt ctaggcacaa agccggcact tggtagcaaa
    18301 gggatttgcc accgcctgtt gtcataatca cgaggcaatc acgattttcc aacacggcat
    18361 tgataacttc ttcttgcccg tctcggaatt tttgatagcc aaaaatatta tttaaaacag
    18421 aaattgcggt ggtgttcatt ggtagttggt tgctacttat gatagtactc gtcttctgag
    18481 agtatctttt aaaatgaaaa taaagcactc atcaaagacg agtgctgtct tagttaacaa
    18541 ttaggcatta gaaagaaacg ctttctccgt gtcgagcaaa agcttcttct aaatgttgcc
    18601 agaacgagcg accttcatct gttacccatt cgccttctac atttttaaag tggaaaccac
    18661 ccaatttgct ggcaagccat aattccagca ttgcctcttg tttattaatg ataacttgcg
    18721 attcatcatc aaaggttaag gtgcagaccg ctccttggat ttcagtatcg actgataatc
    18781 cttcgtcata aattttttct tcaatttgtt gccatacttg ctctacggct tggtggaatt
    18841 gtgctgtgtt cattggtgta tccttatttg aatgttatat aagtagtaaa tacttt
//
LOCUS       DANLXF010000025        18308 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.025, whole genome shotgun sequence.
ACCESSION   DANLXF010000025 DANLXF010000000
VERSION     DANLXF010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 18308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18308
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.025"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            130..1659
                     /gene="mepM"
                     /locus_tag="PXD66_000521"
     CDS             130..1659
                     /gene="mepM"
                     /locus_tag="PXD66_000521"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HDL6318854.1"
                     /translation="MQHVILARDRKRHKKHFKIAVFLLAVFSLFVDIVLALKDQSSIN
                     NTSSQEDIIAIDKQEKYQSINTNLAENNTLHQEQHSSFINYVTDLLTQSALVIEHDDE
                     IVDDQTATSYEDELVGQDDEVEEGIETVENQVPGEKLPEEAEKALEDLLDVADQALRI
                     QNQFSYTVARGDKLKDVLEQSGLTASVAKNLIKQHSALAQLEPGQQFYWVLDNDGELE
                     YMNWLVSEKEERVYERKENGKFSFQRFEKKGEWKLDVVRGTIQNSFAVSLKAVGLSDR
                     QINQLAVGLQSQIATNKLKKGDRFAILIKREYIDGKVTDLGNVEGIHVISSKKSYYAI
                     QADNGRYYSRYGETLSGGFARHPLLFAARVTSSFNPKRRHPITGRISPHKGVDYGVPI
                     GTPIIAPSDGVVEHVAYQARGAGRYIRIKHGHITTVYMHLSKTLVKPGQSVKKGERIA
                     LSGNTGGSTGPHLHYEFHINGRPVNPITVKLPGSNSGMGNKERQAFLNKAKNVESRLK
                     M"
     gene            1669..2352
                     /gene="dsbC"
                     /locus_tag="PXD66_000522"
     CDS             1669..2352
                     /gene="dsbC"
                     /locus_tag="PXD66_000522"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HDL6318855.1"
                     /translation="MKKNLITLGLMCLSTITFASDAKLKSQLEKIGATDVKISDSPLT
                     GFKMATSNEGVVYISQDGRYVIQGKVFELKNGKAIDITNQGLLAELNSYTNEMIVYPA
                     KNEKHVITVFMDITCHYCHLLHTKIKEYNDLGITIRYLAFPRGGMNTQTAKQMEAIWT
                     SADKVKALNDAEDGKLPTQIKTPDIVKKHYQLGIKFGVTGTPNMVTSEGELIGGYVEP
                     KELAKMLSE"
     gene            2437..4158
                     /gene="recJ"
                     /locus_tag="PXD66_000523"
     CDS             2437..4158
                     /gene="recJ"
                     /locus_tag="PXD66_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDL6318856.1"
                     /translation="MEKIIKRRPLLTTEIVSTNPLLNRIYQSRGIKNTQELEHNLKNL
                     HRPQQLANIEQAVDLLLQAFREKSRIIIVGDFDADGATSTSLALIALRQLGFDKVDYL
                     IPDRFSQGYGLSVAVAEMVLAKGADLVLTVDNGISSTEGIALLKSHQIQVLVTDHHLP
                     PESLPNADALVNPNLAFCEFPSKSLAGVGVIFYVMMALRSRMREQHLFENKPEPNFAE
                     LLDLVALGTVADVVPLDQNNRILVHQGLQRIRSGHCRLGIKALAEVGKRDLSTLQAAD
                     LGFAIAPRLNAAGRLENMSLGVELLICENMELARRLALDLDSLNQTRKEFEQEMKAEA
                     LSICANLPSLAQSEQAHGIVLYQADWHQGVIGILASRIKDQFNRPVIAFAQESEESEY
                     LKGSARSINGVHMRDLLEHIDMRHPDLIEKFGGHAMAAGLTIHQSKLDLFKQIFDQSI
                     NAIIEPEQLQGIIYTDGELNALEFTLANAEMIRQGGPWGQHFPEPSFEGEFSILQQKL
                     LAGKHLKLMLQSEKGQLFDAIWFNVDVRAFPDLSIKKARLVYRLDINEFRGEKSLQLK
                     VEYLKYI"
     gene            4286..5134
                     /locus_tag="PXD66_000524"
     CDS             4286..5134
                     /locus_tag="PXD66_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDL6318857.1"
                     /translation="MEKKALFRGFLLSTVALAVAACGNLSKVSDEGTTENPVFPKISE
                     SEFNHDGSQFGSWPNWENVRQIEKGMNKDQLYNLIGRPHFEEGLYGVREWDYAFNYRE
                     NGVHKICQFKILFDKNMNAQSFFWYPNGCNGNSSFALTGDFLFDFDKATLTTKGKEVV
                     DNVAQQLKASKAQQVKVAGYTDRLGSAAYNLDLSQRRSNTVKARLVQQGVTAQIEAVG
                     YGKANQVKACDGETGQALKDCLRPNRRVEISANCGVMKQNEGGNVAGPNGPAPLYQTP
                     AYDGSK"
     gene            5222..6040
                     /locus_tag="PXD66_000525"
     CDS             5222..6040
                     /locus_tag="PXD66_000525"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
                     /protein_id="HDL6318858.1"
                     /translation="MNYQAIAFDLDGTLLSSNATILESSKEAIKKVREKGIKVYFVTG
                     RHHTAVRPYYVELGLDTPVVCCNGTYVYDFKNEKVLSGNPLSAEIASKLVNDAQAQGI
                     HTAVYFEDAMTYESLNIHFTKFQKWVESCAPEVRPNVYQVENFQQEIDKGVTVWKVLI
                     SDPDLAKMQKFVEKLPPDQVSPEWSWVDRVDITSVGNSKGARLAEVLKTEGIDLQKVI
                     AFGDNFNDVSMLETVGLGIAMGNSEPDIQQKAKKTIGSNNEDSIANELKAQFNL"
     gene            6043..7734
                     /gene="narQ"
                     /locus_tag="PXD66_000526"
     CDS             6043..7734
                     /gene="narQ"
                     /locus_tag="PXD66_000526"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HDL6318859.1"
                     /translation="MIKAKYSIARRIGLYFLIMIGFASIISGISLGIMWSNKSDASLI
                     NVSGSLRMQSYRLLSEMDSKKALLPERLLEYHRTLHATELSSLKQSVLLPDSVIESYQ
                     QLLKDWYEMQSYVAQGDKVNYASHIEDYVQRVDDFVFRLQEFAELKLKIATGVIAIAM
                     LLIIALAYIGVWYTRKKIIGPLRQLVKASWQIQNKDFDHVKLAVNEPNELGLLSSTFT
                     EMSNELLKLYTSLEDKVQDKTRRLLAVNRSLLVLYQCSQLLTAKPINKEVLNQVLKTV
                     FDNEHLLGIDLQVYGAEYWNVSIDNKPSLDWVSAEIAIENEKLGILSWKPSLVCPGER
                     LIQNVSEMIGRSLYVVQMQKQQQQLVLMEERAIIARELHDSLAQSLTFFKIQVSLLKL
                     AKTDEKRNEILTVFEKALNDAYSQLRELLTTFRLTIQEANLQQALEKILESLRPKTSA
                     QIRLSCKLPSHMFNAQQQVHVLQIVREAVINAIKHSQATEINVIAETNDDGEHCLTIQ
                     DNGVGLAAHTQPEGHYGLTIMQERAAELKAEFSIENVESGGVKVQVLLPSMISKR"
     gene            7785..8303
                     /locus_tag="PXD66_000527"
     CDS             7785..8303
                     /locus_tag="PXD66_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL6318860.1"
                     /translation="MQFRKAVEADFETILAIYNQAIPTHQITADLELATTQNRRKWFD
                     FHLETAQYPIWVVEDEKGIAGWFSFSPFYERPAFLHTSEISIYLDRYSKGKGYGSKII
                     EFMQAEMLNHNIHTLMAYVFELNTVSQNLMRKHGFEQWGRFPNIANMGKDEKGEDKWR
                     TLLMMSYQKGIE"
     gene            8346..8945
                     /gene="rdgB"
                     /locus_tag="PXD66_000528"
     CDS             8346..8945
                     /gene="rdgB"
                     /locus_tag="PXD66_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HDL6318861.1"
                     /translation="MEKTKIVLATSNADKVKEMADVLSQFGFEVVAQSEFGIESPEET
                     GLTFVENALIKARFAAKMTGLPAIADDSGLSVMALGGEPGLYSARYAGEQATDADNRQ
                     KLLAKMQGQENRLAKFVSCIVFLKHETDPTPYIASGECFGEILTEERGENGFGYDSLF
                     FYPPKACTFAELETKEKKAISHRAIALEQLKQQLAGENK"
     gene            8942..9262
                     /locus_tag="PXD66_000529"
     CDS             8942..9262
                     /locus_tag="PXD66_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HDL6318862.1"
                     /translation="MIITTTPTIEGKQIVEYKGLVFGEVVSGANFIRDFLAGITDIIG
                     GRSGAYESKLNGARKEALAELAKEAQKVGANAVVGVSMEYQSMGGDKGMFIVVATGTA
                     VVIR"
     gene            9266..9718
                     /locus_tag="PXD66_000530"
     CDS             9266..9718
                     /locus_tag="PXD66_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HDL6318863.1"
                     /translation="MQFEQHFEANFWQNKSLLEMNDAEWEALCDGCGKCCYRKYIQGR
                     GRRERLYYTSVACNLLDVNTGRCMNYSNRFKIESDCTKLTKKNLPDFGWLPKTCAYRL
                     LYEGKPLFDWHPLISKDPDSVKNAGMLIANGIHEKDVIDWFEFVIDEV"
     gene            complement(9759..10340)
                     /gene="lpcA"
                     /locus_tag="PXD66_000531"
     CDS             complement(9759..10340)
                     /gene="lpcA"
                     /locus_tag="PXD66_000531"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HDL6318864.1"
                     /translation="MYLEQIKAELQEAADVLDKFMSDEKNIKLIQDAAMLIANSFKQG
                     GKVISCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHLSCVSNDFGYEYVFS
                     RYLEAVGQKGDVLFGLSTSGNSKNVLNAIKVAKEKGMKVIAMTGKDGGQMAGLADVEI
                     RVPHFRYADRIQEIHIKVIHILMMLIEFEMEKA"
     gene            10649..11608
                     /locus_tag="PXD66_000532"
     CDS             10649..11608
                     /locus_tag="PXD66_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL6318865.1"
                     /translation="MVVNPESFEQIIGQSVKIKEVVEQAKKFANLDAPLLIQGETGTG
                     KDLFAKSCHHFGSRRMQKFIAVNCAGLPAEEAESEMFGHRGNGVESVGFFEYAHGGTV
                     LLDSISELSLEMQAKLLRFLNDGSFRRVGEDQEIQVNVRVICTSLQPLSLLVEKGKVR
                     EDLFHRLNVLTLNLPPLRDRKEDIPLLADYFIAQISNQLGVSKLEYDNVFLQALQNYH
                     WRGNLRELYNALYRACTLSHSYRLAVKDLHLPRDMEAMIEVREEATLEEMMSQYEAQL
                     LHQFYAEYPSTRKLAQRLGLSHTAVANKLRQYGLTKNGNSKDE"
     gene            11601..13352
                     /locus_tag="PXD66_000533"
     CDS             11601..13352
                     /locus_tag="PXD66_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL6318866.1"
                     /translation="MNKLAVIFSVCFANLGLNSTAYAAPSIPQELLENSLVYCTTSSG
                     FSFNPQKSDVSSNMNVVTEQIYDKLVEFNPKKNRLEPALAESYSISDDGLVITLRLRK
                     NVAFHTTPWFTPSRKLNAEDVVFSLKRMIGKESDLPEVKSEPESVLHHQRQSYIYQKT
                     AGQIYFPYFESVDLANKIASIHSPSNDIVKIELVRPDSALLAHLASQYAVILSKEYAL
                     QLNADDNLVQLDLLPVGTGVYQLESYENNDYVRLKPNMNYWGKKAYIQNMVVDFSTEA
                     TGRMAKFLNNECDVVAFPEPSQLSVLNNRQLISSKGANLVYLAFNMQRGIGQDLALRQ
                     RIARGINRKRIAEKLFYGTGEVADNLLPTALWSKKNNGSYPYQPHQNSGEQAVEMHDS
                     IINRSLDSKTETEEVNQKQRLKLWVIDEKRVYNQHPLKMAEMIRADLATQNLEVEIRA
                     VSRAYLVQQLANKSADYDLILGGWLANNLDPNSFLEALLSCKAAETVTNLSNWCNEDF
                     DFWLQTARLSDDPASANLLYEFAQKLLEEQLPILPLMNANRVLVIKDGVKQADISPFG
                     QVKLSELRLGLGHKKGN"
     gene            13355..14320
                     /locus_tag="PXD66_000534"
     CDS             13355..14320
                     /locus_tag="PXD66_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL6318867.1"
                     /translation="MLIVFFRKIFLTLITLIFLSLISFNILLRDPLNHLAELGWFEAY
                     CYYVKSLLNGDFGISFSNGEPLTAQILQVFPATITLCLSALIVSLIIGLPLGFFAATQ
                     QKNMVGRLLNTLGSLSLAVPVFWLALVLMYYASLNQWEVSAIGEIHSLYPKQMVTGFL
                     LLDIWLSDVPYKLKMMQSALHHLALPTLVLAVPATLEVMHITQARAGYVMKQNYIKIA
                     KTRGWTPFRVWRTHIICNTLPALLPMIARTFILIFAFGMLIENIFSWGGIGRWLINAL
                     AIQDYNAISAGVVAIGVFVLLVDMVTGFVRVMLDPSDKKDWYGSY"
     gene            14307..15200
                     /locus_tag="PXD66_000535"
     CDS             14307..15200
                     /locus_tag="PXD66_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL6318868.1"
                     /translation="MAVIKEPEQFRETNAFKAFWFHFRQDKLALGSFYLFLLLLALIF
                     VGSLLAPYNYNEQFVGLELMPPSWDDKGQISHFLGTDDLGRDILSRIMYGFYYTLGSA
                     LMITLLIAIIGGMIGIYAGLKKGRSFWLISHLFDTFLFMPILLIAIIIATLMEASLVN
                     AMLAIFLAMLPHFIHKIYQATEKQLKKEYIITLKLDGASCWYLIREVIIPNLTGVAIK
                     ALSHIFVIAILDISALSFIMLGAKSPTPEWGAMIRESMELIYIAPWTVILPGLAIIFS
                     IFTVTILGTRLSVIFEKYRKE"
     gene            15271..16323
                     /locus_tag="PXD66_000536"
     CDS             15271..16323
                     /locus_tag="PXD66_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL6318869.1"
                     /translation="MALLDIRHLSIEIDMPKGRVKMVDNINLTVDEGEICGLVGESGS
                     GKSLIAKVICGMIKDEWIVSADRFRFDDIELLKLSPAERRKLVGDHISMVFQDPLTSL
                     DPSQQIGKQLMQTIQFRGKWWQRFGWKKKKAIELLHRVGIRDHQDIMKSYPSDITEGE
                     AQKVMIAMAVANQPRLLVADEPTNIMESTTQLQVYRLLSSMNQNLGTSILLVSNDMRS
                     IQNWVDSYNVLYCGQTVEIADKESILESSYHPYTRVLLNSSPDFSQPLAFKSSLNTLR
                     GSVPMLQEMPIGCRLGPRCPFAQKKCINKPPLVKVKQHEFACHFPINFREQNLLKNKT
                     ENTPFVVAEIAEKEAF"
     gene            complement(16332..16562)
                     /locus_tag="PXD66_000537"
     CDS             complement(16332..16562)
                     /locus_tag="PXD66_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HDL6318870.1"
                     /translation="MKLTACKLVFLLYLMFNLTACGTVLSLVEQDYRVYAGVIKDFQV
                     MQEGGIFAVLAVIDLPLSFVLDTLMLPVTLSQ"
     gene            16692..17774
                     /gene="leuB"
                     /locus_tag="PXD66_000538"
     CDS             16692..17774
                     /gene="leuB"
                     /locus_tag="PXD66_000538"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDL6318871.1"
                     /translation="MQTYNIAVLAGDGIGPEVMAEAIKVLEATQQKFGFKLNFNQYNI
                     GGAAIDAQGKALPENTLKGCEEADAILFGSVGGPKWTHLLPDEQPERGSLLPLRKHFS
                     LFCNLRPAALYQGLEKFCPLRADIAAKGFDMVVVRELTGGIYFGQPKGRDGEGAQTRA
                     FDTEVYYKYEIERITRVAFESAMKRKKHVTSVDKANVLQSSILWRETVTEVAKEFPEV
                     TLEHMYIDNATMQLIKAPESFDILLCSNIFGDIISDEAAMITGSMGMLPSASLNEQGF
                     GLYEPAGGSAPDIAGKNIANPIAQILSAAMMLRYSFNLGDAADAIETAIQKALADGYR
                     TADLADDSKPLSTSEMGDIIAKNILA"
     gene            complement(17861..18256)
                     /gene="msrB"
                     /locus_tag="PXD66_000539"
     CDS             complement(17861..18256)
                     /gene="msrB"
                     /locus_tag="PXD66_000539"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDL6318872.1"
                     /translation="MIKPIHALTEQQVDILINHGTERPFTGKFLNEHRVGIYRCARCH
                     NDLFHSDTKFDAGCGWPSFYQTISESSLRYLDDYSLGRHRIEIRCGNCDSHMGHVFPD
                     GPPPTGLRFCLNSAALNFKWEETGEEIDG"
BASE COUNT         5835 a         3061 c         3930 g         5482 t
ORIGIN      
        1 gggtattgag ttaaaattag ctgatttttc gtgttgtgac aatagattaa atgaattttt
       61 tcttggtaaa atagtctact cagaaaaata tgtaaatttt agtaaatttt aaaaatagcg
      121 ggatatattt tgcaacatgt aattttagcg agagatcgta agcgacataa aaaacatttt
      181 aagattgcgg ttttcttatt agcggtattt agtctatttg ttgatattgt tctcgcacta
      241 aaagatcaat cctcaatcaa taatacatct tcacaggaag atattattgc cattgataaa
      301 caggaaaaat atcagagtat caacactaat ttggcggaaa ataatactct ccatcaggag
      361 caacattcca gttttattaa ttatgtgact gatttactga ctcagtcggc attagtaatc
      421 gaacatgacg acgaaattgt tgatgatcaa acagcgactt cttatgaaga tgaattagtt
      481 gggcaagacg atgaagtaga agaagggatt gaaacggttg aaaatcaagt tcctggggaa
      541 aaactcccag aagaagctga aaaggcatta gaagatttac ttgatgtggc tgatcaagca
      601 ctacgaattc aaaaccaatt cagttatacc gtagctcgag gtgataaact taaagatgta
      661 ttagagcaat caggcttaac tgcatccgtt gcgaaaaatt taattaagca acattcggca
      721 ttagcacaat tagaaccggg tcaacaattt tattgggtat tagataatga cggtgaatta
      781 gaatatatga actggctggt ttccgaaaaa gaggaacggg tttatgagcg aaaagaaaat
      841 ggtaaatttt cttttcaaag atttgagaaa aaaggggaat ggaagttaga tgttgtaaga
      901 ggaactattc agaatagttt tgcggttagc ttaaaagccg ttggcttatc agaccgccaa
      961 attaaccagc tagcggttgg attgcagtca caaattgcga cgaataaatt aaaaaaaggg
     1021 gataggtttg caattctgat taagcgggaa tatattgacg gcaaagtaac ggatttagga
     1081 aatgtggaag gaatccatgt aattagcagt aaaaaaagtt attatgctat tcaagcagat
     1141 aatggacgct attatagtcg atatggtgaa acattaagtg gaggatttgc tcgtcatcca
     1201 ttattatttg cagcaagagt aacttcttct ttcaacccca aacgcagaca ccctattaca
     1261 gggagaatta gtccgcataa aggggttgac tatggcgtgc caataggtac tccgatcatc
     1321 gcaccaagtg atggtgtcgt agagcatgtg gcatatcaag cccgtggagc aggacgttat
     1381 attcggataa aacacggaca tattacgact gtttatatgc acctcagcaa aacgttggta
     1441 aaaccagggc agagtgttaa aaaaggggag cggatagcat tatcgggtaa tacaggcggt
     1501 tcaaccgggc ctcatttaca ctatgaattc catattaatg gtcgaccggt caacccgatt
     1561 acggtaaaat tgcctggctc aaattcaggt atgggtaata aagaacgtca agcattctta
     1621 aataaagcga agaatgtaga aagtagatta aaaatgtaag gattctcaat gaaaaaaaat
     1681 ctaatcactt taggattaat gtgtttatca accatcacgt ttgcttcaga tgcaaaacta
     1741 aaaagccagc ttgaaaaaat tggagccaca gatgttaaaa tttcagattc tccattaaca
     1801 ggctttaaaa tggcgacttc aaatgaaggt gttgtatata tcagccaaga cggtcgttat
     1861 gtaattcaag gtaaagtatt tgagctaaaa aatggtaaag cgattgatat tactaatcaa
     1921 ggattgttgg cagaattgaa ttcatacact aatgaaatga tcgtatatcc agcgaaaaat
     1981 gagaaacatg tgattacggt gtttatggat attacttgcc attattgcca tttactacat
     2041 actaaaatca aagaatataa tgatttaggt attaccattc gctatttggc attcccgcgt
     2101 ggtggaatga atacccaaac agcaaaacaa atggaagcta tttggacatc agcagacaaa
     2161 gtaaaagcac tgaatgatgc tgaagatggt aaattaccaa cccaaataaa aacgccggat
     2221 attgtgaaaa aacattacca attagggatt aaatttggtg tgacaggtac cccaaatatg
     2281 gtaacttcag aaggtgagtt gattggtggt tatgtagagc ctaaagaatt agcgaaaatg
     2341 ttaagtgagt aattcattaa cttgaataca agcggtcttt ttttgtaaaa tttttgcaaa
     2401 agaagaccgc ttgttgtttc attaaagaag agtattgtgg aaaaaataat taaaaggcgc
     2461 ccattattaa caacggaaat agtctctaca aacccgttat taaatcgaat ctatcaaagt
     2521 cgaggcataa agaatacgca agaactagag cataatttaa agaatttgca tagaccacag
     2581 caacttgcca atattgagca agccgttgac ttgctattac aagcctttcg tgaaaaaagc
     2641 cggatcatta ttgtgggtga ttttgatgca gatggtgcaa ccagtacatc gcttgccctt
     2701 attgcgttac gccaattggg tttcgataag gtagattatt taattccgga tcgtttttct
     2761 caggggtacg gattgagtgt agcggttgct gaaatggtat tagcaaaagg ggcggattta
     2821 gtcttaacgg tagataatgg aatttcttcg actgaaggca ttgctctgct aaaaagccat
     2881 cagattcaag ttttggttac cgatcatcat ttaccgccag agagcctacc caatgcagat
     2941 gcactggtaa accccaattt agctttttgt gaatttccgt ctaaatcttt agccggggta
     3001 ggcgtgatct tctatgtaat gatggcattg cgttctcgta tgagagaaca acatttattt
     3061 gaaaataaac cagagccaaa ttttgctgaa ttactggatt tagtcgcttt aggcacagtg
     3121 gctgatgtag tgccgttaga tcaaaataat cggatattgg tgcatcaagg gttacagcga
     3181 atccgttcag ggcattgccg tttagggata aaagctttag cggaggtggg caagcgagat
     3241 ctttctactt tgcaagcagc agatttgggt tttgctatcg caccacgttt gaatgcagct
     3301 ggtcggttag aaaatatgtc tttaggggtt gagttactaa tttgtgaaaa tatggaatta
     3361 gctcgccggc ttgcgcttga tcttgattct cttaatcaaa cccgaaaaga gtttgaacaa
     3421 gaaatgaaag cagaagcact gtcgatttgt gcgaatttgc caagtcttgc gcagtctgaa
     3481 caggcacacg gaatagtgtt atatcaagca gattggcacc aaggtgtaat tgggatctta
     3541 gcttctcgca ttaaagatca atttaaccgt cctgtcattg cttttgcaca agaaagtgag
     3601 gaaagtgaat atctaaaagg ttctgctcgc tcgatcaacg gagtacatat gcgagattta
     3661 ctggaacata tcgatatgcg acatccagac ttgattgaaa aatttggtgg acacgcaatg
     3721 gcggctggtt tgactatcca tcaatcaaaa ttagatttat ttaaacagat atttgaccaa
     3781 tctattaatg caattatcga gcctgagcaa ttacaaggca tcatttatac cgatggtgag
     3841 ttaaatgcac tagagtttac tttagccaat gccgagatga ttaggcaagg tgggccttgg
     3901 ggacaacatt ttccagaacc tagttttgaa ggggagttta gcattctcca acaaaaatta
     3961 ctcgcaggta aacatttaaa attaatgttg caatcagaga aagggcaact atttgatgca
     4021 atttggttta atgtggatgt aagggcgttt cctgacttat cgatcaaaaa ggcaagatta
     4081 gtttataggt tggatattaa tgaatttcga ggagagaaat ctttgcaatt aaaagtggag
     4141 tatcttaaat atatttgaaa tgttatttta tgtaaattta atagaattaa ttatatttgt
     4201 attgtagaat ccgtccgaat gcaactttaa taggttgtga agctattttt ttatacagat
     4261 tactataagg aaacttaata tgaaaatgga aaaaaaagcc cttttccgtg gatttttatt
     4321 atcgacagtg gcattagcag tggctgcttg tggtaactta agtaaagtga gtgatgaagg
     4381 aacaactgaa aacccggtat tcccgaaaat ctcagaatct gaatttaacc acgatggttc
     4441 acaatttggt tcatggccaa actgggaaaa cgttcgtcaa attgaaaaag ggatgaacaa
     4501 agatcaatta tataatctaa ttggtcgtcc tcattttgaa gaaggcttat atggtgttcg
     4561 tgaatgggac tatgcattca attaccgtga aaatggcgta cataaaattt gccaatttaa
     4621 aattttattc gataaaaata tgaatgcaca aagtttcttc tggtatccaa atggatgtaa
     4681 tggaaactca tcatttgctt taactggcga tttcttattc gatttcgata aagcgacttt
     4741 aactacaaaa ggtaaagaag ttgttgataa tgttgctcaa caattaaaag catctaaagc
     4801 acaacaagtt aaagtagcag gctatacaga ccgtttaggc tctgctgctt ataacttaga
     4861 cttatcacag cgtcgttcta atacagttaa agctcgttta gtacaacaag gcgttacagc
     4921 acaaattgaa gcggtaggtt atggtaaagc gaatcaagta aaagcttgtg atggtgaaac
     4981 tggtcaagca ttaaaagatt gtttacgccc taaccgtcgt gttgaaattt ctgcaaattg
     5041 tggtgtaatg aaacaaaatg aaggtggcaa tgttgcaggt cctaatggtc cggctccact
     5101 ttatcaaacc ccagcatatg atggcagtaa ataatttcta gttttgacta acttagagta
     5161 gaatattaag gcaaccaaat ggttgccttt tcttttatct atttatttat aggggaatac
     5221 tatgaactac caagctattg catttgattt agacggaaca ctactttcat cgaacgcaac
     5281 aattttagaa tccagtaaag aggctattaa aaaggtaaga gaaaagggaa ttaaagtcta
     5341 ctttgttacc ggtaggcacc atactgcggt tcgcccttat tatgtagaac tagggctgga
     5401 tacgccagtt gtgtgttgca atggcactta tgtgtatgat tttaaaaatg aaaaagtatt
     5461 aagcggtaat ccgttatcag cagaaattgc ttcaaaatta gttaatgatg cacaagctca
     5521 agggattcat actgcggttt attttgaaga tgcaatgact tatgagtctt tgaatattca
     5581 cttcactaaa ttccaaaaat gggttgagtc ttgcgcacca gaagttcgtc cgaatgttta
     5641 tcaggtagaa aatttccaac aagaaattga taaaggtgta accgtatgga aagtgcttat
     5701 tagcgatcca gatcttgcca aaatgcaaaa atttgttgaa aaattaccgc ctgatcaagt
     5761 aagcccggaa tggtcttggg ttgatagggt tgatattacc agtgttggta acagcaaagg
     5821 ggcaagatta gcggaagtat taaaaacaga aggtattgat ttacaaaagg tgattgcctt
     5881 tggcgataat ttcaatgatg tttctatgct agaaaccgtt ggattaggca ttgcaatggg
     5941 aaattcagaa ccagatattc agcaaaaagc gaagaaaact ataggttcaa ataatgaaga
     6001 tagtattgct aatgaattga aagctcaatt taatctttaa ttatgataaa agcaaaatac
     6061 tctattgcta ggcggatagg actctatttt ttaattatga tcggatttgc ttcgattatc
     6121 agtggaatct cacttggtat tatgtggagc aataaatctg atgcgagcct gattaatgtt
     6181 tcaggctcac ttcgtatgca aagttatcga ttgttatcag aaatggattc aaaaaaagcg
     6241 ctgctacctg agcgattgtt ggaatatcat cgaacgctac atgctacaga gcttagttca
     6301 ttaaagcaaa gtgttttatt gccagatagc gtgatagaaa gctatcaaca attacttaaa
     6361 gattggtatg aaatgcaatc ttatgtcgct cagggagata aagttaatta tgcttcgcac
     6421 attgaagatt atgtgcagag agttgatgat tttgttttcc gcttacaaga gttcgctgag
     6481 ttaaaattaa agattgcaac aggcgtgatt gcgattgcaa tgttactgat tattgccctt
     6541 gcttatattg gcgtttggta tactcgaaaa aagattattg gacctttaag gcaattggta
     6601 aaagcgagtt ggcaaattca aaataaagat tttgaccacg taaaattagc ggttaatgaa
     6661 cccaatgaat tagggctact ttcctctact tttactgaga tgtctaatga attattaaaa
     6721 ttatatactt cattagaaga taaagttcag gataaaacac gtcgtttatt ggcggtaaat
     6781 cgttctttac tggtattata tcaatgctct caattattaa ctgctaagcc gattaataaa
     6841 gaagtgttaa atcaagtact taaaacggta tttgataatg aacatttgct agggattgat
     6901 ttacaagtat atggggctga atattggaat gtatcaatag acaataaacc aagtttagat
     6961 tgggtttctg ctgaaattgc aatagaaaat gaaaaattgg gtatcttaag ctggaaaccc
     7021 tcactggttt gtcctggtga acgattgatc caaaatgtgt cggaaatgat tggtcgtagc
     7081 ctttatgtag tgcagatgca aaagcaacag caacaattgg tactcatgga ggagcgtgca
     7141 attattgctc gtgagttaca cgattcctta gcccaatctc ttacgttttt caaaattcag
     7201 gttagcctgc ttaagcttgc taaaacagat gaaaagcgta atgaaatttt aactgtattt
     7261 gaaaaagcgt taaatgatgc ttacagccaa ttaagggagc tgttgacgac gtttaggcta
     7321 acgattcaag aagcgaattt acaacaagcg ttagaaaaaa ttttagaaag cttaagaccg
     7381 aaaacctcag cacagattcg cttaagttgc aaattacctt cgcatatgtt taatgctcag
     7441 cagcaagttc acgtattgca aattgtacga gaagctgtga ttaatgctat taagcattcg
     7501 caagccactg aaattaatgt gattgctgaa acaaatgatg atggtgaaca ttgtttgact
     7561 attcaagata atggcgtagg gcttgctgcc catacgcaac cagaagggca ttatggactg
     7621 acgattatgc aagaacgggc ggctgaactc aaagccgaat tttcgattga aaatgtggaa
     7681 agtggcggag taaaagtaca ggttttattg ccgagtatga ttagcaagcg gtaataaaat
     7741 attaattttt tgcgtattat tggcaacgaa taaaaggata acaaatgcaa ttccgaaaag
     7801 cagtagaagc ggattttgag acaatattgg caatttataa tcaagcaatt cccactcacc
     7861 aaattaccgc cgatttagaa ctggcaacta cgcaaaatcg gcggaagtgg ttcgattttc
     7921 accttgaaac agcccaatat cctatttggg tggtcgaaga tgaaaaaggc attgctggct
     7981 ggtttagttt ttcaccattt tatgaaagac cggcattttt acatacttcg gaaatcagta
     8041 tctatttaga ccgctattcc aaaggaaaag gctatggctc aaaaattatt gagtttatgc
     8101 aagctgaaat gcttaatcac aatattcata cgttgatggc gtatgtgttt gaattaaata
     8161 cagtgagcca aaatctaatg agaaaacacg gctttgaaca atggggaaga tttccgaata
     8221 ttgctaatat gggaaaagat gagaaaggtg aggataaatg gcgaacgttg ctgatgatgt
     8281 cttaccaaaa aggtatcgaa taacaagcgg tgatttttta gaatattttt gcaaataagg
     8341 acaatatgga aaagacaaaa attgtacttg caacaagtaa tgcagataaa gttaaagaaa
     8401 tggcagatgt actctcacaa tttggttttg aggtggtggc tcaaagtgaa tttgggattg
     8461 aatcaccgga agaaaccggt ttgacttttg ttgaaaatgc gctgattaaa gcacgatttg
     8521 ctgctaaaat gacaggttta ccggcgattg ccgatgattc aggtttatcg gttatggcac
     8581 ttggtggtga accggggcta tattctgcac gttatgccgg agagcaggca accgatgccg
     8641 ataatcgcca aaaattacta gccaaaatgc aagggcaaga gaatcgtctg gcgaaatttg
     8701 tcagttgtat cgtgttttta aaacacgaaa ctgacccaac accttatatt gcgtcgggtg
     8761 aatgtttcgg tgaaattttg acggaagaaa gaggtgaaaa tggtttcggc tacgattccc
     8821 tgtttttcta cccaccgaaa gcttgcactt ttgccgaatt agaaaccaaa gagaaaaaag
     8881 cgatttcaca ccgtgcaatc gcactagagc aattaaaaca gcaattagca ggagagaata
     8941 aatgattatt acaactacac ctacgataga gggtaaacaa attgtagaat acaaagggtt
     9001 agtatttggc gaagtagttt ctggggctaa ttttatccgt gatttcttag ccggtatcac
     9061 cgatattatt ggcggacgtt caggtgctta tgaaagcaaa ttaaacggtg caagaaaaga
     9121 ggcattagct gaattagcta aggaggcaca aaaagtgggg gcaaatgcgg tggttggcgt
     9181 ttctatggag tatcaatcaa tgggcggcga taaaggaatg ttcatcgtgg tagcgactgg
     9241 tactgcggtt gtaatacgtt aatttatgca gtttgaacaa cactttgaag caaatttctg
     9301 gcagaacaaa tcgctacttg aaatgaatga tgctgaatgg gaagcattat gtgacggttg
     9361 tggtaagtgc tgctaccgga aatatattca gggaagaggg cggcgtgaac ggctttacta
     9421 tacctcggtt gcctgtaatt tattagatgt gaatacaggc cgttgtatga attactcgaa
     9481 ccgttttaaa attgagtcgg attgtacaaa attaactaaa aagaacttac ctgattttgg
     9541 ctggttgcca aaaacctgtg cttatcgttt actttatgaa ggaaagcctc tttttgattg
     9601 gcacccttta atctctaaag atcctgattc tgtaaaaaat gccggaatgc tgattgctaa
     9661 cggtattcac gaaaaagatg tgattgattg gtttgagttt gttattgatg aagtatgaga
     9721 aaaaggcgtt tggtgcaaac gcctttttgt tatagagatt aggctttttc catctcaaat
     9781 tcaattaaca tcatcaaaat atgaatcact ttaatgtgaa tttcttggat gcggtctgcg
     9841 taacggaaat gaggtactcg gatttccaca tcggctaatc ctgccatttg cccgccatct
     9901 ttgccggtca ttgcaatcac tttcatgcct ttttctttgg caactttgat cgcatttaac
     9961 acatttttag agttaccaga ggttgataga ccaaagagta catcgccttt ttgcccaacg
    10021 gcctctagat aacgagagaa gacatattca taaccgaagt cattacttac acagcttaaa
    10081 tggctgacat cggaaatagc gattgccggg tatccgggac ggttttcacg gtaacgacct
    10141 gttaattctt cggcaaagtg catcgcatca cagtgcgaac cgccattacc gcaagaaatc
    10201 accttaccac cttgcttgaa actatttgca atcagcatgg cggcatcttg aattaattta
    10261 atattttttt catcagacat aaatttgtct aatacatctg cagcttcttg aagctcagct
    10321 ttaatttgtt ctaaatacat catttgctcc tttgtttgca aaacgcttac ctttaaacat
    10381 cggctttacc aatgggggta gcgagtaaaa aattcgtttt ttatgaccgc ttgaggcata
    10441 aaaagtgcaa taagtttaga agatctatgc tgtattgtaa acctaataac ttaaataatc
    10501 tgaatagggt tgtaaacaaa agtttccaaa agagaaaaat agcggtatga tcgcctcaca
    10561 atccctctcg cattgttaac tctagtagct aatatcaggc tttaagcgta ttaaacaatg
    10621 cacaaatttg ctatttatag gagactatat ggtcgtaaat ccagaatctt ttgaacagat
    10681 aattggacaa tctgtaaaga taaaagaggt tgttgaacaa gcaaaaaaat ttgccaacct
    10741 tgatgcacca ttattgattc aaggtgagac gggaacagga aaagatttat ttgccaaatc
    10801 ttgtcaccat tttggatctc gtcgaatgca aaaatttatt gccgtgaact gtgccggttt
    10861 accggctgaa gaagcagaat cggaaatgtt cggacatcgt ggcaatggcg ttgagtccgt
    10921 cggtttcttt gaatatgctc acggtggtac agtattatta gactctattt ctgagttatc
    10981 gctggaaatg caagccaaat tattgcgctt tttaaatgat ggctcttttc gtcgagtggg
    11041 cgaagatcaa gagattcaag tcaatgttag ggtgatctgt acttcattac agcctttgtc
    11101 attattagtt gaaaaaggca aagtaagaga agatctgttt catcgtttaa atgttctcac
    11161 tttaaattta ccacctttgc gagatcgaaa agaggatatt ccattattag ctgattactt
    11221 tatcgcccaa attagcaatc agttgggggt gagcaaatta gagtatgata atgttttttt
    11281 gcaagcattg cagaattatc actggcgagg caacttaaga gagctttata acgcacttta
    11341 tcgagcttgt acactttcgc atagctaccg tttagcggta aaagatcttc atttacccag
    11401 agatatggag gcgatgattg aagtacgtga ggaggcgaca cttgaggaaa tgatgagcca
    11461 gtatgaagcc caattattgc accaatttta tgcagaatat ccgagtaccc gaaaattagc
    11521 acaacggctc ggactttcac atactgcggt ggcaaacaaa ttgcgtcaat atgggttaac
    11581 aaaaaatgga aacagtaaag atgaataaac tagcggtcat tttttcagta tgttttgcaa
    11641 atttagggct aaattcgacc gcttacgcag cgccaagtat tcctcaagag ctacttgaaa
    11701 atagccttgt gtattgcact acttcatcgg gattctcttt taacccacaa aaatccgatg
    11761 tgagttcgaa tatgaatgtg gtaacagagc agatctacga taaattagtt gagtttaatc
    11821 cgaaaaaaaa tcgactagag cctgctttag ctgaaagtta ttcaatcagc gacgatggtt
    11881 tagtgattac attacgttta agaaaaaatg tcgcttttca tacaactcca tggtttactc
    11941 cgagtcgtaa attaaacgca gaagatgtgg tgttttcgct aaaacgaatg attggtaaag
    12001 aaagtgattt acctgaagtg aaaagtgagc ctgaaagtgt attacaccac cagcgacaaa
    12061 gctatattta ccaaaaaaca gcggggcaga tttattttcc ttattttgaa agtgttgatt
    12121 tagcgaataa aattgccagc attcattctc cgtcaaatga cattgtaaaa attgaattag
    12181 tccgtcctga ttcagcattg ttggcgcatt tagccagcca atatgcagtt attctgtcta
    12241 aagaatatgc tttacagctc aatgcagatg ataacttagt tcaactggat ttattaccgg
    12301 taggaacagg cgtatatcaa cttgaaagtt atgaaaataa tgattatgtg cgtttaaaac
    12361 caaatatgaa ttactggggc aaaaaagcat atattcaaaa tatggtcgta gatttttcca
    12421 cagaagcgac cggcaggatg gcaaaatttt taaataatga atgtgatgtg gttgcttttc
    12481 cggaacctag ccaattatca gtgttaaata atcggcagct tattagtagt aaaggtgcta
    12541 atttggttta tttagcattc aatatgcaac ggggtattgg gcaggatttg gcattacgcc
    12601 aacgtattgc acgcggcata aatcgcaaac gtattgcaga gaaattattt tatggtacag
    12661 gtgaagtagc ggataaccta ttaccaacag ctttgtggtc gaaaaaaaat aatggaagct
    12721 acccttatca accacaccaa aattcaggtg agcaagcggt tgaaatgcat gacagtatta
    12781 taaatcggtc tttagatagc aaaacagaga ccgaagaagt aaaccaaaaa cagcgcttaa
    12841 aactgtgggt aattgatgaa aaacgagttt ataatcaaca cccattaaaa atggcagaaa
    12901 tgatacgtgc tgatttagct acgcaaaatt tagaggtcga aattcgcgca gtaagtcgag
    12961 cttatttggt gcaacagctt gctaataaaa gtgcggatta tgatttgatt ttaggtggtt
    13021 ggttagcaaa taatcttgat cctaacagtt ttttggaggc cttactcagt tgtaaagctg
    13081 cggaaacggt gactaattta tcaaattggt gcaatgaaga ttttgatttt tggttacaaa
    13141 cagcccgtct aagtgatgat ccggcatcag ctaatttatt gtatgaattt gcacaaaaat
    13201 tattagaaga gcaattgcct attctccctt taatgaatgc taatcgtgta ttagtcataa
    13261 aagatggagt gaaacaggca gatatttccc ctttcggcca ggtaaaatta tcagagttac
    13321 gtttgggctt ggggcataaa aaaggaaact aattatgctg attgtctttt ttcgaaaaat
    13381 ttttctaacc ttgattacgt tgattttttt gtcacttatc agttttaata ttctgttacg
    13441 agatccatta aaccacttag ccgagttagg ctggtttgag gcttattgtt attatgtaaa
    13501 atctctgcta aatggtgatt ttggtattag tttcagtaat ggagaaccgc tcacagcgca
    13561 aattttacag gtgttcccgg ctacaattac gctttgttta tcggctctca ttgtttcttt
    13621 aattattggt ttgcctcttg gtttttttgc ggctactcag caaaaaaata tggttgggcg
    13681 attattaaat acactaggtt cattgagttt agccgtgccg gtattttggc tggcattagt
    13741 gctaatgtat tacgcttctc tcaatcagtg ggaagtgtca gcaattggag aaatccattc
    13801 gctttatccg aaacagatgg taaccggctt tttgctatta gatatttggt tatctgatgt
    13861 accttataaa ttaaaaatga tgcagagtgc tttacatcac ttagctttgc cgacattagt
    13921 cttggctgtg ccagctacat tagaagttat gcatattact caagctagag caggttatgt
    13981 aatgaaacaa aattacatta aaatagcgaa aacgcgtggc tggacaccgt ttagagtttg
    14041 gagaacgcat attatttgta atactttacc tgcattatta cctatgattg cccgtacctt
    14101 tattttaatt tttgccttcg gtatgttaat tgagaacata tttagctggg gaggaattgg
    14161 acgttggctg ataaatgccc ttgctattca agactataat gcgatttcag cgggagtagt
    14221 tgcaatcggg gtgtttgtgt tattagtaga tatggttaca ggctttgtgc gggtgatgtt
    14281 agacccatca gataaaaagg attggtatgg cagttattaa agaacccgag caatttagag
    14341 aaaccaacgc atttaaggca ttttggtttc atttccgaca agataaactt gcacttggca
    14401 gtttttatct ttttttgcta ttactggctc tcatttttgt tggtagtttg cttgctcctt
    14461 acaattataa tgaacaattt gttggtttag aattaatgcc tccctcttgg gacgacaaag
    14521 gtcaaatcag ccatttttta ggcactgacg atttaggacg ggatatttta agccgtatta
    14581 tgtatggttt ttactacacg cttggttccg cactgatgat taccttatta attgcgatta
    14641 tcggcggtat gatcgggatt tatgccgggc tgaaaaaagg acgttcattt tggttgatta
    14701 gccatttgtt cgataccttt ttatttatgc cgattttatt gattgcgatc atcattgcaa
    14761 ccttaatgga agcgagtttg gtcaatgcaa tgttggcgat tttcttagcg atgttgccgc
    14821 attttatcca taaaatttat caagcaaccg aaaaacaact caaaaaagag tacatcatta
    14881 ccttaaagct agacggtgca agctgctggt atttaattcg agaagtgatt attcctaatc
    14941 ttaccggcgt ggcaattaaa gcactttcac atatttttgt gatcgccata ttagatatta
    15001 gtgcgttaag ttttattatg ctgggggcta aaagcccgac ccctgaatgg ggagcgatga
    15061 ttcgtgaatc catggaactg atttatatag ctccttggac ggtgatatta cctggcttag
    15121 caattatttt tagtattttt accgttacaa tacttggtac acgcttaagc gtgatttttg
    15181 aaaaataccg taaagaatag tatttaaata ggatactgac acataacaag cggtcatttt
    15241 taagtaaaat tttacagatt aaggaatagt atggcattac ttgatattcg ccatttaagt
    15301 attgaaattg atatgccaaa aggcagagta aaaatggtgg ataacatcaa cctgactgtt
    15361 gatgagggcg aaatttgtgg cttggtggga gaatctggtt cgggaaaaag cctcatagcc
    15421 aaagtgattt gcggtatgat taaagatgaa tggattgtga gtgcagatcg ctttcgcttt
    15481 gacgatattg aattactgaa actttcgcca gcggaacgcc gaaaattggt aggcgatcat
    15541 atttcaatgg tattccaaga tccgcttact agccttgacc caagccagca aattggtaaa
    15601 caactgatgc aaaccattca atttagaggg aagtggtggc aacgatttgg gtggaaaaag
    15661 aaaaaagcaa tcgagctact acaccgtgtt ggtattcgtg atcatcaaga tattatgaaa
    15721 agttacccaa gcgatattac cgaaggcgaa gctcagaaag tgatgattgc aatggcggtg
    15781 gcaaatcagc cccgattatt ggtggcagat gagccgacta atattatgga atcaaccacg
    15841 cagttgcagg tttatcgctt gctttccagt atgaatcaga atttgggaac ctcaatcttg
    15901 ctggtgagta atgacatgcg tagcattcaa aattgggttg attcttacaa tgtgctttat
    15961 tgtggacaaa ccgttgaaat tgcggataaa gagagcattc ttgaatcgtc ttaccacccc
    16021 tatactcgag tgttactcaa tagttcgccg gacttttctc agcctttagc ctttaaaagt
    16081 agcttaaata cgctacgagg ctcagtcccg atgttacaag aaatgccgat tggttgtcgt
    16141 ttaggccctc gttgcccttt tgcacaaaaa aaatgtatca acaaaccgcc attagtgaaa
    16201 gtaaaacagc acgagtttgc ctgccatttt ccgattaact ttagagaaca aaatttgctt
    16261 aaaaataaaa cggaaaatac gccgtttgtg gtggctgaaa tagcggaaaa agaggcgttt
    16321 taatgtggtt attattggct aagtgtaacg ggtaacatta aagtatctaa tacgaaactc
    16381 aagggtaaat cgatcaccgc aagcaccgcg aatataccgc cttcttgcat tacttggaaa
    16441 tcttttatta cgccggcata aacacggtaa tcctgttcaa ccaagcttag tactgtgcca
    16501 caagcggtga ggttaaacat caaatagagt agaaaaacga gtttacaagc ggtcaatttc
    16561 atcaaaaaat ctccaaatac gcttttattt tacgctattt ttatcgggtt ggcgtaaaat
    16621 tcgttttaaa aacagctatt ttcaggcaga atgtagccga atcgacatta aatatactaa
    16681 gaggatctta tatgcaaact tacaacattg cagtgcttgc cggtgacggc attgggccgg
    16741 aagtgatggc agaagctatc aaagtgcttg aagcgactca acaaaaattt ggctttaaac
    16801 taaattttaa tcaatataac attggtggag cagcgattga tgctcaaggt aaagcgttac
    16861 ccgaaaatac cttaaaaggc tgtgaagagg cagatgcgat tttgtttggc tcggtaggtg
    16921 gcccgaaatg gacgcattta ctacctgatg agcaacctga acgaggttcg ctattaccac
    16981 tgcgtaaaca tttctcgcta ttctgtaatt tacgccctgc tgcattgtat caagggctgg
    17041 aaaaattctg cccattacgt gccgatattg ctgctaaagg ttttgatatg gtggtggtgc
    17101 gtgaattaac cggcgggatt tattttggac agccgaaagg ccgtgatggt gaaggtgcgc
    17161 aaacgagagc gtttgacacc gaagtatact acaaatatga aatcgagcgt attacccgtg
    17221 tggcgtttga atctgcaatg aaacgtaaaa aacacgtaac ctctgtggat aaagccaatg
    17281 tgttacaaag ctcgatttta tggcgtgaaa ccgtcacgga agtagctaaa gagttcccag
    17341 aagtcacgct tgagcatatg tatattgata atgcgacaat gcagctaatc aaagcacctg
    17401 aatcgttcga tattttatta tgctcaaaca tcttcggcga tattatttcc gatgaggctg
    17461 caatgattac aggttctatg ggcatgttgc cttcagccag cctcaatgag caaggtttcg
    17521 gcttatatga accggcaggc ggttcagcac cggatattgc cggtaaaaat attgcgaatc
    17581 cgattgcgca aattttatca gccgcaatga tgttgcgtta tagctttaac ttaggtgatg
    17641 ctgctgatgc gattgaaaca gcgatccaaa aagcccttgc tgatggctac cgtaccgctg
    17701 atttagctga tgacagcaaa ccgctttcta cctcagaaat gggcgatatt attgcgaaaa
    17761 atattctcgc ttaaggctag aagttaaata gcacaagcgg tcattattcg ccaaattttt
    17821 gcaaattttc cgctaataat gaccgcttgt ttattactcg ttagccgtca atctcttcgc
    17881 ctgtttcttc ccacttaaaa ttcaatgccg cagagttgag gcaaaaacgc aaccctgttg
    17941 gtggtgggcc gtccggaaaa acgtgtccca tatgcgaatc acaattccca caacgaattt
    18001 cgatacgatg acgtcctaaa ctgtaatcat ctaagtaacg tagtgacgat tctgaaatcg
    18061 tttggtaaaa acttggccag ccacaaccgg catcaaattt agtatctgag tgaaataaat
    18121 cgttatggca tctggcacaa cgataaattc ctacccgatg ttcattcaaa aattttccgg
    18181 taaacggacg ttccgtaccg tggttgatta aaatatcaac ttgttgttct gtcagggcgt
    18241 gaataggttt aatcatcagc aattctccta agatagtagc gaagtcattg tagctttttt
    18301 tgacctga
//
LOCUS       DANLXF010000026        18122 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.026, whole genome shotgun sequence.
ACCESSION   DANLXF010000026 DANLXF010000000
VERSION     DANLXF010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 18122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18122
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.026"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(72..4058)
                     /gene="hrpA"
                     /locus_tag="PXD66_000540"
     CDS             complement(72..4058)
                     /gene="hrpA"
                     /locus_tag="PXD66_000540"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HDL6318873.1"
                     /translation="METPLQKNHKKPPLVKLENITTADFRRLLGRLKGAENIKNIDHK
                     RSVLAEISQDLTACQARFFARTQHAQNLKIDYPDLPVSARREEILKLISENQVVVIAG
                     ETGSGKTTQLPKMCLELGRGVKGLIGHTQPRRIAARSVATRIAEELKSELGETVGYKV
                     RFNDQVSENSLIKLMTDGILLAEIQHDSYLNQYDTLIIDEAHERSLNNDFILGYLKQI
                     LHKRPDLKVIITSATIDVERFSKHFNNAPIIEVSGRTFPVEVRYRPIVEDQDQLQGIL
                     NAVDELQAEGRGDILIFMNGEREIRDTAEALQKQELRHSEILPLYARLSAAEQQRIFQ
                     PSGGLNRIILATNVAETSLTIPNIKYVIDTGTARISRYSYRTKVQRLPIEPISQASAN
                     QRKGRCGRTSEGICIRLYSEEDFNSRPQFTDPEILRTNLAAVILQMTSLGLSDIANFP
                     FVDAPDSRQIQDGIRLLEELQAIIPNEKIPSTACGGGLGRGEIRSNDGTPPLPTSPRK
                     RGDGFRLTPIGRQLAQLPVDPRLGRMVIEAAKNGSLHEVMMIVSALSIQDPRERPQEK
                     QQSADDKHRRFADKESDFLAFVNLWHFIQAQQKELSKNQFRNLCRKDYLNYNRVREWQ
                     DIYHQLRLAVREMGLKINSEPASYQPIHTALLAGLLSHIGLKDNEKMHYLGARNAHFF
                     IFPNSALFKKQPKWVMAAELVETTKLWGRMVARIEPEWVEPLAVHLTKSSYSEPHWAK
                     SKGAVMASEKVSLYGIPIVAQRSVNYGSIDPTVSREIFIRSALVEGECHNNYKFFKEN
                     HRLIREVEALEHKSRRRDILVDEQVLFEFYDQRIGTEVVSSKHFDTWWKQASKKDPEL
                     LNFEKSFLINDNANLASELDFPNFWHQGDLKLKLSYQFEVGKDHDGVTVHIPLPLLNQ
                     VEPEGFDWQIPGLRHELIVSLIKSLPKSLRRNFVPAPNYADAFLGRAEPFKKPLLDNL
                     SHELRRMTGVLVEPEQWDLSQLPPHLRMTFRVIDDKGKKLQESENLDELKFALKDEVQ
                     NTLSNIADDGIEQSGVHIWNFAELPQFYEQKKAHFSVKAYPAIVDEQTAVGVKLFETE
                     FEQARAMQAGLRRLLLLNVPSPIKYLHEKLPNKAKLGLYFAPFGKVLELIDDCIACAV
                     DQLVEEFGGFVWTEEKFTQLHEFVRANLNDKTVEIAKQVEKCLTLAYELNKRLKGKLD
                     FTMAFALSDIKTQIEGLIYPDFVTKTGYARLADLYRYLTAIERRLDKLLVDSNTDRAK
                     MLRVEAVQEAYKQLLAKLPKSKAVPDEVLEIRYMIEELRVSLFAQQLGTKYPISDKRI
                     LNVINEIK"
     gene            complement(4162..4614)
                     /locus_tag="PXD66_000541"
     CDS             complement(4162..4614)
                     /locus_tag="PXD66_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDL6318874.1"
                     /translation="MTLQFNAVALLLVVLIILGLISQNSAVTISAAVLLIMQQTLLSK
                     FIPFVDQYGLKIGIIILTIGVLSPLVSGRITLPELAQLLNWKMALSIIAGVLVAWLGG
                     RGVNLMGSQPVLVTGLLIGTVIGVAFLKGVPVGPLIAAGILSLIVGKT"
     gene            complement(4677..4946)
                     /locus_tag="PXD66_000542"
     CDS             complement(4677..4946)
                     /locus_tag="PXD66_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318875.1"
                     /translation="MDAAMSLRFDKLRFVKRLQEANQTPEMAEAFANALDEALEQSQS
                     PLATKADLQLELEKLKNEINTTIFKAITLNITILGFLMAIMKFIN"
     gene            complement(5044..5640)
                     /gene="grpE"
                     /locus_tag="PXD66_000543"
     CDS             complement(5044..5640)
                     /gene="grpE"
                     /locus_tag="PXD66_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005712850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDL6318876.1"
                     /translation="MTTQNEHLQEEVKQEEIQPEVVTEETQAEEQNTPQEEPLALAQA
                     RIAELEAYIAEADKREQDIQLRAQAEIQNIRRRAEQDVEKAHKFALEKFSKELLTVVD
                     NLERGLDALDKAVTDETTQALVDGVEMTHKEFINTLAKFGVVAIGEVGEAFNPELHEA
                     ISMQPAEGIEANHVSTVLQKGYTLHGRVIRPAMVMVAN"
     gene            5862..6956
                     /locus_tag="PXD66_000544"
     CDS             5862..6956
                     /locus_tag="PXD66_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL6318877.1"
                     /translation="MKKLAGLFAAGLATVALTACNEEKAAEPKATAAEAKPQATNQTV
                     HLYTWTEYVPEGLLDEFTKETGIKVEVSSLESNETMYAKLKLQGKDGGYDVIAPSNYF
                     VSKMAKEGMLAELDHAQLPVIKELNQDWLNKPYDQGNKYSLPQLLGAPGIAFNTADYK
                     GETFTSWGDLWKPEFAGKVQLLDDAREVFNIALLKLGKNPNTTNPDEIKEAFEELKKL
                     RPNVLSFTSDNPANSFIAGEVSVGQLWNGSVRIAKKENAPVDMVFPKEGPVLWVDTLA
                     IPANAKNKENAHKLINYLLSAKVAEKLTLAIGYPTSNVEALKVLPKEITEDTAIYPSA
                     EVLQKSQWQDDVGDAIELYEKYYQELKAAK"
     gene            7092..8483
                     /locus_tag="PXD66_000545"
     CDS             7092..8483
                     /locus_tag="PXD66_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDL6318878.1"
                     /translation="MNLRWQEYPENARKLFKLSLPIFISQLSASGMGLADIVMAGLVS
                     DDDVSAIAVSNSIYFPLFLFVSGLLNAITPTVSYLNGSSNRHLIANQVRQGYWIVFAM
                     SIPLMAIFLNSHLILDLMQTPAEFSIKSQQYLAVMAVGLIPALLAVSLRCMNDGLSNP
                     KPAMRITFFGLLLNIPLNYIFIFGKFGLPEMGAVGCGVATAIVNLIMFFMLLHYCYTN
                     SAQKDIRLFEKWIDAPSGQILLKLCKLGLPIAFAIFTEVMLFSTSSLFLSPLGSQVVA
                     SHQAALQTSSLFFMIPMSFGIATTIMIGQTLGQKNVEGAKILSYHALITATALAIIAA
                     IIIILLKNFIPFAFTNNPISIAITAHLLIFAAVYQIPDAIQAVANGILRGYKHTQPIL
                     YVTLFCYWGVSMPLGYILARTDWIVQPMAAQGFWLVFCIGLSLAGGLLLWQMRKIQAV
                     PKAQLIAKLERIK"
     gene            8542..12066
                     /gene="recB"
                     /locus_tag="PXD66_000546"
     CDS             8542..12066
                     /gene="recB"
                     /locus_tag="PXD66_000546"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HDL6318879.1"
                     /translation="MKNLNPIQLPLNKSVLIEASAGTGKTFTIANLYLRLLLGIGCNP
                     LTVEQILVVTFTKAATEELKNRIRKNIQQCADFLKDQADGLEVESTKSYRNNLDFLAQ
                     IYPLIPNIHEALLRLSIAEREIDTASVFTIHGFCQKMLVQFAFESGVRFDLDLQPNQS
                     DLLKKLSEEVWREQFYPQDLAITYAVAEQLGTPEYALNAVRRYLSTELPEPNASLNQD
                     IAEHLAQYQQFINEVKSYWKTHSDELVETIRHDLKHKITLHGGWYRATTFEAGVEVMN
                     QWANSQHNHFPAGLYLFTTEKIAAGTKKSCEPIDSPHLAKNQAFLTAYKERFENSLSA
                     MLQYQFLIALKAKLAEYKQSHPQRSFDDLLTMLNQALKNLHTGEALSQKIRAMYPFAM
                     IDEFQDTDLTQYEIFSQIFMAENAENGFIMIGDPKQSIYKFRGADIFTYLRAAKQADE
                     QFTLGKNWRSLPEVVQAVNNLFSFANDNSPFLYEDIGFHPVEADSQKGQLVSEQGHFV
                     GYITENSKLDDLAEMCAYQIHQQLEAMAQGKFGVEKNGEFQSFAAKDIAILVRKGSQA
                     KRIKKALAKRNIKSVYLAESASVFGSEIATELCWLLQAALNPYHYKALLSALGSTLWG
                     LTAAEIHRYKENEQLWDLKVNQFVEYSNIWKEQGVLPMLHKIYLEQGIIERLKGLENA
                     DRLITDLLHLTEILQETSQALESEAELLRWYEKQVNSPDFNDEQQLRLESEEELIKIV
                     TIHGSKGLQYPIVWLPFVSQKSEGDTQTALSLYRNSQGALDWCLGSEEASFSLQYEAY
                     AEDLRLLYVALTRAESQLNLVLPKEISGWSPLHYLLADGEIPTGKVPVSDYFSQKRIA
                     ANLVEVAGELPFSLWEGEQSSDDYQAKTFSGNIRVVGQITSFTSLYAQHERFSEGKSE
                     RVMDLAQDRDVHIETIEAGYSSETAAFSPFQFPHGIKVGTVLHHFFEHCQFNEQIDRE
                     AVAKVCEQLGLSEEWIEPTALWFERILTTPLAEANFCLKAIDETKRLNEWQFYLRLKN
                     DKALHQLNALLKQHSPLAKTLPELQLPQLEGFVRGFVDCIVQVEEKFYLIDYKSNFLG
                     YLPQNYAKEHIQREMGRQRYDLQYLLYTLALHRYLTARLGEKYDYERDFGGVAYLFLR
                     AMNGENDNGVYFDKPTKQLIEEMDRLFG"
     gene            complement(12108..12590)
                     /gene="greB"
                     /locus_tag="PXD66_000547"
     CDS             complement(12108..12590)
                     /gene="greB"
                     /locus_tag="PXD66_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HDL6318880.1"
                     /translation="MAKSNYITRSGWQALDQELKFLWREERPKVTQAVSDAAALGDRS
                     ENAEYIYGKRRLREIDRRVRFLSKRLEVLQIVDYHPKQEGKVFFGAWVELENEEGEAK
                     QYRIVGCDEFDPAKNWISIDSPVARALIGKQVDDEIRVETPLGKVLLYINKIWYEQAS
                     "
     gene            complement(12641..13921)
                     /gene="pepB"
                     /locus_tag="PXD66_000548"
     CDS             complement(12641..13921)
                     /gene="pepB"
                     /locus_tag="PXD66_000548"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HDL6318881.1"
                     /translation="MTAMKISLSTQNAPEIWGKNAILSANDAGFTVHNPNNDLATIQK
                     AARKIKNQGISTVELTGDSWDLESCWAFHQGFHSVKNQGSVQYPDLDEQQAEFDNRVK
                     CATFVRELINKPSDKLTPEKLAEQAVEFIQKMAKPNTVSYQIIRGEALQEQGYHGIWT
                     VGKGSANPPAFLVLDFNPTNDENAQVLACLVGKGITFDTGGYSLKPSDSMSTMRTDMG
                     GAALLTGSLAFAIARGLNKRVKLYLCCAENMVSHNAFKLGDIITYRNGVTVEVLNTDA
                     EGRLVLADGLIDACKQNPQFIIDAATLTGAAKVAVGNDYHSVLSMDDNLTEALFKSAK
                     TENEPFWRLPFEEFHRGQIASSFADIANIGSVPVGAGASTATAFLSYFVENYQQNWLH
                     IDASATFRKTASDLWAAGATGLGIRTVANLLLSK"
     gene            complement(14060..14881)
                     /locus_tag="PXD66_000549"
     CDS             complement(14060..14881)
                     /locus_tag="PXD66_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010869033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HDL6318882.1"
                     /translation="MKNIKLSAMSLTALFVLTACSHTHKNSDEHHQMVLQEQATLGIN
                     WMQQSGEYQALAHQAFNAAKVAFDQAKVKKGKKKAVVVDLDETMVDNSLYAGWQVKNH
                     KPFDGESWTRWVNARQTGAIAGAVEFNNYVNSHKGTMFYVSNRKDSSEKAGTIDDMNK
                     LGFSGVSEQTLLLKKDKSNKTPRFEEIEKQGYEIVLYIGDNLNDFGDATYRKSNAERR
                     AFVAENSQQFGKKFIMLPNPNYGDWEGGLDKDYYKGNAESRVKIRHNAIKAWDGK"
     gene            15029..15972
                     /locus_tag="PXD66_000550"
                     /pseudo
     CDS             15029..15972
                     /locus_tag="PXD66_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
     gene            complement(16085..17248)
                     /locus_tag="PXD66_000551"
     CDS             complement(16085..17248)
                     /locus_tag="PXD66_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDL6318883.1"
                     /translation="MKRKTLATLVLIAALGIGGYFYYQQQTNKPQIHYITQAVTRAEI
                     NKNVLATGSVRANKRTEVGAQVSGKIQKLYVELGQSVKQGDLIADIDSSNQSNTLSTA
                     QARLSSYKAQLTSAQVALEVAQSNYNRLNKLYRANSASLNDLETAKNTLASAKATVDN
                     IKAQLKSAEISVNDAKTNLTYTQIISPMDGVIVSVPVSVGQTVNSNQTSPTIVQVADL
                     SKMLIKLEISEGDIAQVKVGQKVKFSTLAEPNREYSATIDSVDPALTTLTDNNYTEQS
                     GNTEAVYYYANVSVDNADNSLRIGMTTQAQITIDKREQVLVVPTSVIKKRGKENYVLV
                     LENEQSVERKIQIGISDTQNTEVLAGLNEGDQVIVTQRADDEKISTARGPRMF"
     gene            complement(17314..>18122)
                     /locus_tag="PXD66_000552"
     CDS             complement(17314..>18122)
                     /locus_tag="PXD66_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL6318884.1"
                     /translation="PFRAIISDLDGTLLNANHKIGDFTIDTLEQLSKKGVDIFFATGR
                     NHPDVSQIIGKVNVKNAMLVTSNGARANNLEGKKILNHYLPEDLAFELMNIEFDDQNV
                     CLNSYQGDEWFINKEIAELKKFHQDSGFSYQVVNFAHHHGRNTEKVFFIGKTAEALVP
                     IEHYIRDTYGDRVQMTYSALLCLEVMAKGVCKANALAELVKLRGYTLADCIAFGDGMN
                     DVEMLSLAGKGCLMGNADPRLKAVLPNNEVIGFNKDEAVASYLRATFGIV"
BASE COUNT         4897 a         3796 c         3939 g         5490 t
ORIGIN      
        1 gtattcgaga aaacaagcgg tcagagttgc aaaatttttt acttatctga ccgcttgttg
       61 tatagggaat attactttat ttcattgatc acattcaaaa tccgcttatc cgaaatcggg
      121 tattttgtgc cgagctgttg agcgaacaga ctgacccgca gttcttcgat catatatcga
      181 atttccagta cctcgtccgg caccgctttg gattttggca gtttggcaag gagctgttta
      241 tatgcctctt gcactgcttc cacccgtagc attttggctc ggtcggtgtt gctgtccacc
      301 aacagtttat ccaaacggcg ttcgatagcg gtcaaataac ggtataaatc tgcaaggcgg
      361 gcgtagccgg ttttggtgac aaagtcgggg taaatcagcc cttcgatttg ggttttgatg
      421 tcgcttaacg caaacgccat cgtaaaatcg agcttgcctt ttaaccgctt gtttagctcg
      481 taggccagtg tgaggcactt ttcaacctgt ttggcaattt ctacggtttt atcgtttaaa
      541 tttgcccgca caaattcgtg cagttgggtg aatttttcct ccgtccagac aaacccgccg
      601 aactcttcta ccagctgatc taccgcacag gcaatgcagt cgtcaatcag ctccaatact
      661 ttgccgaacg gggcaaaata taagcccagc ttggctttgt tcggcagttt ttcgtgtaga
      721 tatttaatcg gcgatggcac atttagcaac agcagacggc gaagtcctgc ctgcattgca
      781 cgagcctgct caaattccgt ttcaaacagt ttcaccccca cagccgtttg ttcatcgaca
      841 atcgcagggt aggctttcac gctgaaatgg gcttttttct gctcatagaa ctgtggcaat
      901 tcggcaaaat tccaaatatg cacaccactt tgctcaatgc cgtcatcggc aatgttggaa
      961 agggtgttct gcacctcatc tttcaaggca aatttcagct catcaaggtt ttcgctctct
     1021 tgcagtttct tgcctttatc atcaatcaca cggaaagtca tccgcaaatg tggcggcagt
     1081 tggcttaaat cccactgttc aggctcgacc aatacacccg tcattcggcg tagttcgtgg
     1141 cttagattgt ccagcaacgg ttttttaaac ggctcggctc gccctaaaaa ggcgtccgca
     1201 taattcggcg ccggcacaaa atttcgccgt agcgatttcg gtaaagattt aatcagcgag
     1261 acgatcagct cgtggcgtag ccccggaatt tgccaatcga aaccttcggg ttccacttgg
     1321 ttaagcagcg gtagcggaat atgcaccgtc acgccatcgt gatcttttcc gacctcaaac
     1381 tgatagctca gtttgagttt taaatcgcct tgatgccaga aattcgggaa atccagctcg
     1441 ctggcaaggt tggcgttgtc gttgatcagg aacgattttt caaagttcag cagttcagga
     1501 tcttttttgc ttgcctgttt ccaccaagta tcaaaatgct tgctcgacac aacttcggtg
     1561 ccaatccgct gatcgtaaaa ctcaaacagc acctgctcgt ccaccaaaat atcacggcgg
     1621 cgggatttgt gttccaatgc ctcaacttca cggatcaagc ggtgattttc tttgaaaaat
     1681 ttgtaattgt tgtgacattc gccttccacc aacgccgagc ggataaaaat ctcacggctg
     1741 acggttgggt ctatcgagcc ataattcacc gagcgttgtg ccacaatcgg aatgccgtag
     1801 agcgacactt tttccgatgc cattaccgct ccttttgact tcgcccagtg tggttcgctg
     1861 taactggatt tagtgaggtg tactgccaac ggttccaccc attccggctc aatgcgagcg
     1921 accattcgtc cccaaagttt ggtagtttcc accagctctg cggccattac ccatttcggc
     1981 tgttttttga aaagtgccga gttcgggaaa atgaaaaagt gggcatttct tgctcctaaa
     2041 tagtgcattt tttcattatc tttcaagccg atatgcgaca acaagccggc gagcagggcg
     2101 gtgtgaatcg gctgataact tgccggctca ctgttaattt ttaagcccat ttctctaact
     2161 gcaaggcgga gttggtggta gatatcctgc cactcccgca cacggttgta gttcaaataa
     2221 tccttacggc agagattacg gaactggttt ttcgacagtt ctttttgttg ggcttggata
     2281 aagtgccaca aattcacaaa ggctaagaaa tcgctctctt tatcggcaaa acggcggtgt
     2341 ttatcgtctg ccgactgctg tttttcctgc ggacgctcac gagggtcttg gattgagaga
     2401 gctgacacaa tcatcatcac ttcgtgcaac gaaccgtttt tcgccgcctc gatcaccatt
     2461 ctgcctaagc gaggatcgac tggcagttgg gcgagttggc gaccgattgg ggttaatcta
     2521 aacccatccc cccgtttacg gggggaggtt gggagggggg gagtgccatc attagaacgg
     2581 atttcccccc tccctagccc tcccccgcaa gcggtggagg ggattttttc atttggaata
     2641 atcgcctgta attcttccaa caaccgaatc ccgtcctgaa tttgtcggct gtcaggggca
     2701 tcaacaaacg ggaaattggc aatatcactc aatccaagcg aagtcatttg taaaataacc
     2761 gctgcaaggt tagttcgtag aatttcagga tcggtaaatt gtggacggct gttgaaatcc
     2821 tcttctgaat agaggcggat acagatccct tccgaggttc gcccacaacg ccctttccgc
     2881 tggttggcgg aggcttgcga aatcggctca atcggcaagc gttgcacttt ggtgcggtag
     2941 ctataacgag aaatacgagc cgtgccggtg tcgatcacat atttaatatt cggaatagtg
     3001 agggaggttt ctgccacgtt ggtcgccaga ataattcggt ttagcccacc gcttggctgg
     3061 aaaatccgtt gttgttcggc agccgataag cgggcgtaaa ggggtaaaat ttcgctatgt
     3121 cgcagttcct gcttttgcaa ggcttcggca gtgtcacgaa tttcccgttc accgttcata
     3181 aaaatcagaa tatcgccacg cccttcggct tgcagttcgt ccaccgcatt tagaatacct
     3241 tgcagttgat cttgatcttc cactatcggg cgataacgca cttccaccgg gaatgtccga
     3301 ccagagactt caataatcgg ggcgttgttg aaatgtttgg aaaaacgttc tacatcaata
     3361 gttgccgagg tgatgatcac tttcagatcg gggcgtttgt gcagaatttg tttcagataa
     3421 cccaaaataa aatcgttgtt cagcgaacgc tcgtgagctt cgtcaataat cagcgtatcg
     3481 tactgattta aatagctgtc gtgctggatt tccgccagca gaataccgtc cgtcattagc
     3541 ttgattaagc tgttttcact gacttgatcg ttaaagcgga ctttgtaacc gaccgtctcg
     3601 cccagttccg atttcagctc ttcggcaatg cgagtcgcca ccgaacgtgc ggcaattcgg
     3661 cggggctggg tatgcccaat caagcccttc accccacgcc cgagttctaa gcacattttc
     3721 ggtaactggg tggttttgcc cgaaccggtt tcccctgcga tcaccaccac ctgattttcg
     3781 ctaatcagtt ttaaaatttc ctcacggcga gcggaaacag gcagatcagg gtagtcaatt
     3841 ttcaggtttt gggcgtgctg agttcgtgcg aaaaaacgtg cctgacaagc ggtgagatct
     3901 tgcgaaattt ctgccaaaac tgaccgcttg tgatcgatgt ttttaatatt ttccgcccct
     3961 tttaagcgac cgagcaggcg gcggaaatcg gcggtggtga tattttccag cttcacaagc
     4021 ggtggtttct tatgattttt ttgcaaaggt gtttccattt aatttctctt ttgcattaag
     4081 accttgtgat ttattgtagg gtgggcgtaa gcccacgtaa cagaataggg attttatgcg
     4141 tggacttacg tccaccctac attaggtttt accaacaatc agtgacaaaa tccctgccgc
     4201 aatcaacggc ccgaccggta cgccttttaa gaacgccacg ccgatcaccg tgccgattaa
     4261 aagcccagtt accaacaccg gctggctgcc cattaaattc acgcccctgc cacctagcca
     4321 cgctaccagc acgccagcaa ttattgagag tgccattttc cagttcaata gttgggcaag
     4381 ttcgggaagc gtaattcgcc ctgaaaccaa ggggctaagt acgccgatgg ttaaaatgat
     4441 aatcccgatt tttaagccgt attgatcgac aaaggggata aatttagaaa gtaaggtttg
     4501 ttgcataatc agcaatacgg cggctgaaat agtaaccgca ctgttttggc tgatcagccc
     4561 taaaataatc agcaccacaa gtagaagtgc cactgcgtta aattgtaaag tcataggatt
     4621 aaaacgtaaa aacccttact tcatagaagt aagggagtgg ttaattaaac gttgagctag
     4681 ttaataaatt tcattattgc cattaaaaag ccaagaattg tgatatttaa ggtaattgct
     4741 ttaaatatcg tggtattgat ttcatttttt agcttttcaa gttcaagttg caaatcagct
     4801 tttgttgcta gtgggctttg actttgctct aaggcttcat cgagagcatt agcaaatgcc
     4861 tcagccattt caggtgtttg attggcttct tgcaagcgtt ttacaaaacg taatttgtca
     4921 aatcgtaggc tcatagctgc atccatttca attatcctct tgtggttaag ttataaacta
     4981 atttagcata atcacaagca ggaattttag gagattttgt gatcttcgtc gaaaatcgac
     5041 aatttagttt gccaccatca ccattgccgg gcgaatcact cgtccgtgaa gcgtgtagcc
     5101 tttttgtagc actgtgctga catggttggc ttcaattcct tcggcaggtt gcattgaaat
     5161 agcttcgtgc aattcagggt taaatgcctc gcctacttcg ccaattgcca ccacaccaaa
     5221 tttagccagt gtattgatga attctttatg agtcatttca acgccgtcaa ctaacgcttg
     5281 agtggtttca tcggtcacgg ctttatcaag ggcatctaat ccacgttcaa ggttatcaac
     5341 cactgttaac aattctttgg agaatttttc taaagcgaat ttgtgagctt tttcaacatc
     5401 ttgctctgca cggcggcgga tattttgaat ttctgcttga gccctaagct gaatatcttg
     5461 ctcacgtttg tcggcttctg caatataggc ttctaactcg gcaattcgag cttgagcgag
     5521 agctaacggt tcttcttgag gtgtattttg ttcttccgct tgagtttctt cagttacaac
     5581 ttcaggttga atttcttctt gttttacttc ttcttgtaag tgttcatttt gagtggtcat
     5641 tttttatcct ttaaaagact aatctggaaa tgtaaatctt ccttaatata gcgataaatt
     5701 aagggatttc aagcatatct ctttcttttt gcgaaaataa ttcacttttt aatcagaaaa
     5761 ggcttgcaaa atacgggggg gggcttaaaa tgccctagtc ttttccgccc ttaggcaagg
     5821 ctatattttt taatcacttt tctttaacgg agaaaactta aatgaaaaaa ttagcgggtt
     5881 tatttgcagc aggtttagca acagttgcat taacagcgtg taatgaagaa aaagcggcag
     5941 agccaaaagc aactgcggct gaagcaaaac ctcaagcaac aaatcaaaca gttcaccttt
     6001 atacttggac tgaatatgtg cctgaaggtt tgttagatga atttacgaaa gaaaccggca
     6061 ttaaagttga agtttcaagt cttgaatcta acgaaacaat gtacgctaaa ttaaaattac
     6121 aaggcaaaga tggcggttat gatgtaattg ctccatcaaa ctactttgta tctaaaatgg
     6181 cgaaagaagg aatgttagcg gaattagatc acgcacaact tccggttatt aaagaattaa
     6241 accaagattg gttaaataaa ccttacgatc aaggaaacaa atattcatta cctcaattat
     6301 taggtgcgcc gggtattgca tttaatacgg cagattacaa aggtgaaaca ttcacttctt
     6361 ggggtgattt gtggaaacct gaatttgcag gtaaagtaca attattagat gatgctcgtg
     6421 aagtattcaa cattgcgtta ttaaaattag gtaaaaaccc aaatacaact aatccggatg
     6481 aaatcaaaga agcctttgaa gagttgaaaa aattacgtcc aaatgtgctt tcatttacct
     6541 ctgataaccc ggcaaactca tttattgctg gtgaggtttc tgtaggtcag ttatggaacg
     6601 gttctgtacg tattgcgaag aaagaaaacg caccggttga tatggtattc ccgaaagaag
     6661 gtcctgtgct ttgggtcgat actttagcca ttccggcaaa tgcgaaaaac aaagaaaatg
     6721 cacataaatt aatcaactac ttattaagtg caaaagtagc tgaaaaatta acgttagcga
     6781 ttggttaccc aacttcaaac gtagaagcat taaaagtatt accgaaagaa attactgaag
     6841 atacagcaat ttacccatct gcagaggttt tacaaaaatc tcaatggcaa gatgatgttg
     6901 gtgatgcaat tgagctttac gaaaaatact accaagagtt aaaagcagca aaataatagc
     6961 tgattaattc tgaagcctct agggagcaat ctttagaggc tttctctttt gagttgcaaa
     7021 aaatttggca aaaacgaccg ctttctaagg tacaatcata aaaacatact tagattttga
     7081 gagattaaac catgaactta agatggcaag aatatcctga aaatgcccgc aaattattta
     7141 aactatccct acccattttt atttctcagc tttcagcttc cggaatgggg ctggcggata
     7201 ttgtaatggc tggcttggtg agtgatgatg atgtctcggc gattgcggta agtaattcga
     7261 tttactttcc gctctttctg tttgtgtcag ggttgctgaa tgcaattacc ccaaccgttt
     7321 cttacttaaa cggctcgagc aatcgccatt taattgctaa ccaagtaagg cagggctatt
     7381 ggattgtgtt tgcaatgtct attccattaa tggcaatttt cctaaacagc catctgattt
     7441 tagacttaat gcaaacaccg gctgagtttt caatcaaatc gcaacaatat ttagcagtaa
     7501 tggcagttgg gcttattcct gctttgctgg cggtgagttt acgttgtatg aacgatggat
     7561 tatctaaccc taaacctgcg atgcggatta cctttttcgg tttattgctg aacattccgc
     7621 taaactatat tttcattttc ggcaaatttg gcttgcctga aatgggagcg gtaggctgtg
     7681 gtgtagcaac cgcaattgtg aacttgatta tgttttttat gttgttgcat tattgctaca
     7741 ccaattctgc ccaaaaagat attcgtttgt ttgagaaatg gattgatgct ccaagcggtc
     7801 aaattttgct aaaactttgc aaattagggc tgccgattgc ctttgcgatt tttaccgaag
     7861 tgatgctgtt ttcgacctca tcgctgtttc tgtcaccact tggttcacag gtggtggcta
     7921 gccaccaagc tgcattgcaa accagctcgc tattttttat gattccgatg tcgtttggca
     7981 ttgcaaccac cattatgatc ggacaaacac ttgggcagaa aaatgtggag ggggcgaaaa
     8041 tcttaagtta ccacgcactg attaccgcaa ccgctttagc tattattgcc gccatcatta
     8101 ttattttgtt gaaaaatttt atcccttttg cgtttaccaa caacccgatt tcgattgcga
     8161 ttaccgctca tttattgatt tttgcggcgg tttatcaaat accggatgcc attcaagcgg
     8221 tagcaaacgg cattctgcgt ggttataaac atacccaacc gattttatac gttactctgt
     8281 tttgttattg gggggtgagt atgccgctgg gctacatttt ggcaagaacc gattggattg
     8341 tacaaccaat ggctgctcaa ggtttttggt tagtcttttg tatcggctta agtttggctg
     8401 gtggcttgtt attatggcaa atgcgaaaaa tacaagcggt gccaaaagca caattaattg
     8461 caaaattaga acggattaaa taacagattc tctgctatgg aacatagcag tgctttttac
     8521 ttttagaaga taactttgct gatgaaaaac ctaaatccta tccaactacc tttgaataaa
     8581 tccgttttaa tcgaagcgtc ggcaggtaca gggaaaacct ttaccattgc caacctgtat
     8641 ttgcgtttat tgctcggcat tggttgcaac ccgttaacgg tggaacaaat tttggtggtg
     8701 acatttacca aagcagcaac cgaagagttg aaaaaccgta tccgtaaaaa tattcagcaa
     8761 tgtgccgact ttttaaaaga tcaagctgat ggtttggaag tggaaagtac aaaatcctat
     8821 cgaaacaacc ttgatttttt ggcacagatt tatccgctca ttccgaatat tcacgaagca
     8881 ttgttacgtt taagtattgc cgaacgggaa attgataccg ccagcgtgtt taccattcac
     8941 ggcttttgtc aaaaaatgct ggttcagttt gcgtttgagt cgggcgtgcg gtttgacttg
     9001 gatttacagc caaaccaaag tgatctactt aaaaaattaa gtgaagaagt ctggcgagag
     9061 caattctatc cgcaagattt agccatcact tatgcggttg ccgaacaact tggtacgccg
     9121 gaatatgccc tcaatgcggt aaggcgttat ttatctactg aattacctga accaaatgcc
     9181 tctttaaatc aagatattgc cgagcatttg gctcaatatc agcagtttat taatgaggtg
     9241 aaatcctatt ggaaaacgca ttcagatgaa ctggttgaaa ctatccgcca cgatttaaag
     9301 cataaaatta cgctgcacgg aggttggtat cgagcgacca cttttgaagc cggtgtggaa
     9361 gtgatgaacc agtgggctaa ttcgcaacat aaccatttcc cggcagggct ttatttattc
     9421 actacagaga aaattgcggc tggcacgaaa aagagctgtg agccgataga ttcccctcat
     9481 cttgcaaaaa atcaggcttt tttgaccgct tacaaggaac gttttgaaaa cagcttgtca
     9541 gcaatgttgc agtatcaatt tttaattgct ctcaaagcaa aattggcaga gtacaaacaa
     9601 tcgcacccac aacgcagttt tgatgattta ctcacaatgc taaaccaagc gttgaaaaat
     9661 ctacacacgg gcgaagcctt gtctcagaaa attcgggcga tgtatccttt tgcgatgatc
     9721 gatgagttcc aagataccga tttaacccaa tatgaaattt tcagccaaat ttttatggca
     9781 gagaatgcag agaacggctt tattatgatt ggcgacccga aacagtcgat ctataaattc
     9841 cgtggggcgg atattttcac gtatttgcga gcagcaaagc aggctgatga acaattcaca
     9901 ttgggcaaaa actggcgttc gttgcctgaa gtggtgcaag cggtcaataa tttgttttct
     9961 tttgcaaatg ataactctcc gtttctttat gaggatattg gctttcaccc agtggaggct
    10021 gatagccaaa aaggtcagtt agttagtgag caagggcatt ttgtaggcta catcacggaa
    10081 aacagtaaat tagatgattt ggctgaaatg tgtgcttatc aaattcatca acagcttgag
    10141 gcgatggctc aaggcaaatt cggagttgaa aaaaacggcg aatttcaatc ctttgctgca
    10201 aaagatattg cgattttagt ccgaaaaggt tcacaagcta agcgaatcaa aaaggcgttg
    10261 gcaaaacgta atatcaaatc cgtctattta gctgaatcag cttctgtatt tggttcggaa
    10321 attgcaacag aattgtgttg gttattgcaa gccgcactga atccgtatca ttacaaggca
    10381 ttattatcgg cgttaggctc aaccttgtgg ggcttaacgg cggcagagat ccaccgctat
    10441 aaggaaaatg agcaactttg ggatctgaaa gtcaatcagt ttgtggaata cagcaacatt
    10501 tggaaagagc agggcgttct gccgatgttg cataaaatct acttggagca gggcattatc
    10561 gaacgtttga aaggtttgga aaatgcggat cgcttgatta cggatttatt gcatttaacc
    10621 gaaattttgc aggaaacctc gcaagcgtta gagtctgaag ctgaactgtt gcggtggtat
    10681 gaaaagcaag ttaattcgcc cgattttaat gacgagcaac aacttcggtt ggaaagtgaa
    10741 gaggagctaa tcaaaattgt gacgattcac ggctcgaaag gcttgcaata tccaattgtt
    10801 tggttacctt ttgtttcaca aaaaagtgag ggagacacgc agacagcatt aagtttatat
    10861 cgcaattctc aaggtgcgct ggattggtgt ttaggctcag aagaggcaag tttttcgcta
    10921 cagtatgagg cttatgcgga agatttacgt ttgctgtatg tggcattaac ccgcgcggaa
    10981 tctcagctta acttagtgtt gccgaaagag atttccggct ggtctccact ccattacttg
    11041 ttagcagacg gggaaatacc tacaggaaaa gtgcctgttt cagattattt tagccaaaaa
    11101 cggattgctg ctaatttggt tgaagtagcc ggggaattac ctttctcact gtgggaagga
    11161 gaacaatctt cagacgatta ccaagcaaaa acctttagtg gcaatatccg tgtggttggt
    11221 caaattacca gctttacttc actttatgcc caacacgagc gttttagtga agggaaatca
    11281 gaaagggtaa tggatttagc ccaagatcga gatgtacata tagaaaccat tgaggcaggt
    11341 tattcaagtg aaactgccgc tttctcgcca ttccaattcc cacacggtat taaagtgggc
    11401 acggtgttac atcacttttt tgagcattgc caatttaacg aacaaattga tagagaggct
    11461 gttgctaaag tatgcgagca attagggcta agcgaggaat ggattgaacc aacagcatta
    11521 tggtttgagc gtattctaac tacccctttg gcagaggcaa atttctgctt aaaagcgatt
    11581 gatgaaacca aacgattaaa cgagtggcaa ttctatttac gcttaaaaaa tgataaagcc
    11641 ttacatcaat taaatgcgtt attaaagcaa catagccctt tagcaaaaac tttacctgaa
    11701 ttgcaattac cgcaattaga aggttttgtg cgaggctttg tagattgtat cgtgcaagtg
    11761 gaggagaaat tctacttaat tgattacaaa tcgaatttct taggctatct gccacaaaat
    11821 tatgcgaaag aacacattca acgggaaatg ggcagacaac gctatgattt gcaatatctg
    11881 ctttacactc tcgccttaca ccgttatcta actgcccgtt taggtgaaaa atacgattat
    11941 gaacgagact tcggcggagt ggcttacttg ttcttaaggg caatgaacgg cgaaaatgat
    12001 aacggcgttt attttgacaa gccgactaag cagttgattg aagaaatgga tcggttgttt
    12061 gggtaaatat gtgtaggggc gaaaattttt cgccccttta tgggtaatta actcgcttgc
    12121 tcataccaaa tcttattgat ataaagcaac actttaccaa gcggagtttc taccctaatt
    12181 tcatcatcaa cctgtttgcc gattaaggct cgggcgacag gggagtcaat cgaaatccag
    12241 ttttttgctg ggtcgaactc atcgcaacca acaatacggt actgttttgc ctcgccctct
    12301 tcattttcta attccaccca agcgccgaaa aagactttgc cttcttgttt agggtgataa
    12361 tcaacaattt gtagcacctc tagccgctta gataaaaaac gcacacggcg gtcgatttct
    12421 ctcaaacggc gtttgccgta aatatattcg gcattttcgc ttcgatcacc caatgcggca
    12481 gcatcagaca ccgcttgagt cactttcggg cgttcttccc gccatagaaa ttttaattcc
    12541 tgatctaagg cttgccagcc tgaacgggta atgtaatttg atttagccat agttatttaa
    12601 ttgtttatgg agcaaatagt gatttgcccc tacgaagttt ctattttgaa agcaataaat
    12661 tcgctactgt tcttattccc agtccggttg ctcctgccgc ccataaatcg ctcgccgttt
    12721 tgcggaatgt ggcagaggcg tcaatatgta gccagttttg ctggtagttt tctacaaagt
    12781 aagataaaaa tgcggtggcg gtacttgccc cagcccccac cggcacagag ccgatattgg
    12841 cgatgtctgc aaaggaagaa gcaatttgcc cacggtgaaa ttcttcaaac ggtaaacgcc
    12901 agaacggttc attttccgtt ttggcagact taaatagtgc ttcggttagg ttgtcgtcca
    12961 ttgaaagcac cgagtggtag tcattgccga ctgcgacttt tgctgctcct gtgagggttg
    13021 cagcatcaat aataaattgt ggattttgct tgcacgcatc aattaaacca tcggcaagca
    13081 ctaaacgccc ttcagcatcg gtgtttaaaa cttcaacagt tacgccattg cggtaagtga
    13141 taatatcgcc taatttgaac gcattgtggc tgaccatatt ttccgcacag cagaggtaga
    13201 gtttcactcg tttattcaag cctcgagcaa tggcaaaagc aagtgagccg gttaaaagtg
    13261 ccgctccgcc catatcggtt cgcattgtgc tcatactatc gcttggtttt agactataac
    13321 cgccggtgtc aaaggtaatg cctttgccga ccaagcaagc cagtacctgt gcgttttcat
    13381 cgttggttgg gttgaagtct aatactaaga atgcaggcgg attggcagag ccttttccaa
    13441 ctgtccaaat gccgtgatag ccttgttctt gtaatgcttc gccacgaatg atctggtaag
    13501 aaaccgtatt cggttttgcc attttttgga taaactcgac cgcttgttcg gctaattttt
    13561 caggggttaa tttatctgat ggcttattaa ttaattcacg cacgaaagtg gcacatttta
    13621 cacggttatc aaattcggct tgctgttcgt ctaagtctgg gtattgtacc gagccttgat
    13681 tttttaccga atgaaatcct tgatgaaacg cccagcagga ttctaaatcc caactatcgc
    13741 cggtcagctc cacggttgaa ataccttgat ttttaatttt gcgggcggct ttttgaatag
    13801 tggctaaatc gttgttcgga ttatgtacgg taaaccccgc atcatttgcg gaaagaatag
    13861 catttttacc ccaaatttca ggggcgtttt gagtagaaag tgaaattttc attgctgtca
    13921 ttatcggttt ccttagaaaa tggattttgc tttatcctaa caaacttttg caaagtttta
    13981 tacaaaaatg accgcttgca gagcatagcc ttgacaagcg gtcattgttt cgtgttttta
    14041 caacctgatt aaggacggat tatttgccgt cccaagcttt aattgcattg tggcggattt
    14101 taacacggct ttcagcattg cctttgtagt aatctttatc taaaccgcct tcccagtcgc
    14161 catagttcgg gttcggtaac ataatgaatt ttttaccgaa ttgttggctg ttttcggcta
    14221 cgaatgcacg gcgttccgcg tttgatttac ggtaagtcgc atcgccgaaa tcgtttaagt
    14281 tgtcgccaat atagagcacg atttcatagc cttgtttttc gatttcttca aagcgagggg
    14341 ttttgttcga tttgtctttt ttcaacaaga gtgtttgttc gctcacacca ctaaagccta
    14401 atttgttcat atcatcaata gtaccggctt tttcactgct gtctttacgg tttgaaacat
    14461 agaacatagt tcctttgtgg ctattaacgt agttgttaaa ttccactgca ccggcaatcg
    14521 ctccggtttg gcgggcattt acccaacgag tccagctttc accatcaaac ggcttgtggt
    14581 ttttaacctg ccagccggca taaaggctgt tgtccaccat agtttcatct aaatctacta
    14641 ccacggcttt tttcttgcct tttttcactt tggcttggtc gaatgccact ttcgctgcat
    14701 tgaatgcttg atgggcaaga gcttggtatt cgccggattg ttgcatccag tttatcccta
    14761 atgtagcttg ctcttgcaac accatttgat gatgctcatc gctgttttta tgcgtgtgcg
    14821 aacaagcagt taatacaaat aaagctgtga ggctcatagc agaaagtttg atgtttttca
    14881 tacgtatatc cttagtttta ttgttgataa acagagcgat tatacctaaa aagagttgct
    14941 tatggctaat agtttattgc tcctattcac ttttaaagca aactaaggta gcctagtaac
    15001 ctttttataa caattacaca gaggctatat gcaaacatta ttaaaattta cacaatttat
    15061 cagcaaaacc ttcgccattt gggtggtgtt ttttgctttt atcgcagcac aatttccgga
    15121 aacctttaag caattcctgc cttggattcc ttatctgtta gggattgtga tgttgggaat
    15181 ggggttaacc ttaactttca aagattttgc cgaggtaacg aaaaatccga aatcagtgat
    15241 tatcggcgtg atcgctcaat tcgtggtatg ccgagtgtag cttatggatt ggcaaaagca
    15301 tttaacttac ccgcagattt agccattggt gtgattttag ttggttgttg cccgggcggt
    15361 acttcttcaa acgtgatgac ctatttggct cgtggcaaca cagcgttatc ggtagcttgt
    15421 accactattt caactttact tgccccagtg ctcacgccgg caattttcta tttatttgcc
    15481 agccaatggt tagaaattaa tgcaacggca atgtttattt cggtgttgca aatggtgcta
    15541 ttgccaattt ttgtgggcgt ggtgattcgt actattttca aacaaaaagt agagcaattt
    15601 agccaaacaa tgccattaat ttcagtgatt gcaatcgtat taattgtcac ggcggtggtg
    15661 tcagttagcc gagatcgtat tattgaatcc ggtttattaa tttttgcggt agtagcatta
    15721 cacaacggtt taggttattt aatcggttat ttggcgagcc gtttattaaa attaccgatt
    15781 gcagacagca aagcggtggc gattgaagtg gggatgcaaa actcaggatt gggggcagca
    15841 cttgccgcat tacactttaa agccaaccct atcattgcgg tgccaagtgc ggtatttagc
    15901 ttctggcaca atatttcagg cccgattttg gcgatgattt tcgctaaaat gaaaaacgat
    15961 aacgaaaaat aaaattcgtt ttaatacaga cgttttaatg ttttaataaa gaatagacaa
    16021 acggttaaat ttttctaatt ttttgcaaaa tgttcagaaa atttaaccgc ttgttgttta
    16081 gaatttagaa cattcttggt ccacgtgcag tgctgatttt ttcatcatcc gctctttggg
    16141 taacaatcac ttgatcaccc tcatttaagc ctgctaatac ttcggtattt tgcgtatccg
    16201 aaatgccaat ttggattttt ctttcaaccg actgctcatt ttccagcacc aacacataat
    16261 tctctttacc acgttttttg attacgctgg tgggtacaac taaaacctgt tcccgtttat
    16321 caatagtaat ttgggcttga gtagtcattc cgatgcgtaa actgttatcg gcattatcaa
    16381 cggaaacatt agcgtagtaa taaaccgctt cggtattacc cgattgttcg gtgtagttat
    16441 tatcggtcag cgtggtgagt gccggatcaa ctgaatcaat ggtagcacta tattctcgat
    16501 ttggctcagc aagggtggaa aacttaactt tttgcccgac tttaacctgt gcaatatcgc
    16561 cctccgaaat ttcaagttta atcagcatct tagataaatc tgctacctgt acaatagttg
    16621 gcgaagtttg attggaattg accgtttgcc cgacagaaac cggtacagac acaatcacgc
    16681 cgtccattgg cgagataatt tgcgtataag ttaagttggt tttagcatcg tttaccgaaa
    16741 tttcagccga ttttaattgt gctttaatgt tatccaccgt ggctttggct gaagctaagg
    16801 tatttttagc cgtttcaaga tcgtttaaag aggcactatt tgcccgatag agtttattta
    16861 agcggttgta gttcgattga gctacttcaa gtgcaacctg tgcagaggtg agctgtgctt
    16921 tataagaaga aagccgggct tgagcggtgc ttaaggtgtt gctctggttg ctggaatcaa
    16981 tatctgcaat taaatctcct tgcttcaccg attgcccgag ctcgacatag agtttttgga
    17041 ttttgcccga aacttgagcc cccacttcgg tgcgtttatt cgctcgaacc gaaccggttg
    17101 ccagcacatt tttgttaatt tctgcccgtg tgacagcttg ggtaatgtaa tggatttgcg
    17161 gtttattggt ttgttgctga taataaaaat agccgcctat tccgagagcg gcaattagca
    17221 caagagtggc taatgtttta cgtttcatac tgttccttga tgatatacaa gcggtcaaat
    17281 ttagacaaaa atttgcaaaa aagttgctct caactaaaca atgccaaatg tggctcgaag
    17341 atagcttgcc accgcttcat ctttgttaaa gccaatcact tcattattcg gtaatacggc
    17401 ttttaaacgt ggatcggcat tacccattaa acaacctttg ccggctaaac ttagcatttc
    17461 cacatcattc ataccgtcac caaaagcgat acaatcggct aaggtgtagc cccgtaattt
    17521 gaccaattcg gcgagagcat tggctttaca cactcctttt gccatcactt caaggcaaag
    17581 tagggcagag taggtcattt gcacacggtc gccataggta tcccgaatat aatgttcaat
    17641 cggtactaaa gcttcagcag ttttgccgat aaaaaacact ttctcggtat ttcgaccgtg
    17701 atgatgagca aaatttacta cttgatagct aaatcctgaa tcttgatgga atttttttaa
    17761 ctctgcaatc tctttattga taaaccattc atcgccttga tagctgttta agcacacatt
    17821 ttggtcgtca aattcaatgt tcattaattc aaaggcaaga tcttccggta aataatggtt
    17881 gagaattttt ttaccctcta aattgttggc tcttgccccg tttgaagtga ctaacattgc
    17941 atttttaacg tttactttcc caataatctg agacacatct ggatgattgc gaccggtggc
    18001 gaaaaaaata tcaacacctt tttttgatag ttgttctaat gtatcaatag tgaaatcacc
    18061 aattttatga ttggcattta atagtgtgcc atcaagatcg gaaataattg cacgaaatgg
    18121 ga
//
LOCUS       DANLXF010000027        17980 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.027, whole genome shotgun sequence.
ACCESSION   DANLXF010000027 DANLXF010000000
VERSION     DANLXF010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 17980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17980
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.027"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..245
                     /locus_tag="PXD66_000553"
     CDS             <1..245
                     /locus_tag="PXD66_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HDL6318885.1"
                     /translation="IVAVTIGANNDQLITFNYIFAQSELRLSTLVAILFGLGLILGWL
                     VTGIFYLKIKFQNVALNRRVKRQAQQITELTTPKAE"
     gene            249..1445
                     /gene="lapB"
                     /locus_tag="PXD66_000554"
     CDS             249..1445
                     /gene="lapB"
                     /locus_tag="PXD66_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HDL6318886.1"
                     /translation="MLELLFLLLPIAALYGWYMGQRSAKKDQETASNKFSREYVTGLN
                     FLLSNQQEKAVDLFLSMLQKQESENHIASESQFEAELTLGNLFRSRGEVDRALRIHQT
                     LVNSPHYSFDQKLLAKQQLAKDFMVAGFYDRAENYYISLVDEPEFAKYSLSQLAVIYQ
                     KTREWKKAINVTEKRLRIEPDADKIPLSHFYCEYAQGVKFDDKAAFIESLNKALEYYP
                     QSTRASIILGDFYLEEGEYLRALSYFEQILVQDPDYISEVLGRIKQSYMALEDLNGYE
                     LFLIRANRVKHNSSVDIALTEFIEEKDGINAAHSKLYQQLSTYPNLITFHRFIRYQAD
                     FAEEGNGKESLVLLHNMVGNQIKRSFQYRCLNCGYQSYRLMWQCPSCNQWEKIKPVQS
                     IEGIIQ"
     gene            1469..2161
                     /gene="pyrF"
                     /locus_tag="PXD66_000555"
     CDS             1469..2161
                     /gene="pyrF"
                     /locus_tag="PXD66_000555"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDL6318887.1"
                     /translation="MNNKVIVALDYETEREALEFVDLVDPSLCRLKVGKEMFTTLGTN
                     FVKQLHERKFDVFLDLKYHDIPNTVARAVRSAADLGVWMVDLHASGGLAMMEEAKKIL
                     EPYGKEAPLLIAVTVLTSMEDLDLLQIGINASPMEQVIRLAHLAQRAGLDGVVCSPQE
                     VEVLRTHCGQDFKLVTPGIRPKGTDFGDQRRVMTPKQAVETGSDFLVIGRPITQAQDP
                     LAVLKSINQSIS"
     gene            2244..2555
                     /gene="yciH"
                     /locus_tag="PXD66_000556"
     CDS             2244..2555
                     /gene="yciH"
                     /locus_tag="PXD66_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HDL6318888.1"
                     /translation="MTLVYSTETGRIKSEVIKEERPKGDGIVRIQRQTSGRKGKGVCL
                     ITGLDLEDKALNSLASELKRKLGCGGSFKDGVIEIQGDNRGFIKQILEQKGFKVKLAG
                     G"
     gene            2658..4820
                     /locus_tag="PXD66_000557"
     CDS             2658..4820
                     /locus_tag="PXD66_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HDL6318889.1"
                     /translation="MWKKTLIAVFLASQYAFAVGTGKEPSKSYTETELKQLKNDWLAI
                     YQKELQYAENLRLKQRENFQQINYLADAAISQKNLSLSTEQIIQRLLLTLAGYPLEMK
                     AEWKLLNAKITLKQATESEVKAFISKYPDSLYQRQLQNLMFELLYREQKFPELLEYAQ
                     NVKQSSLADQCRLFSARYELAASKAQINPEVSQVTEQKAENAELLALVQEFDRFWLAT
                     PKLTSDCANLESYWRDQGLKTDEKMRLKAVELIKQNANAELANLAANVNNENLKIWLS
                     SVEKVANNPADLQNFIENQPLDSPFYADNKAIIQQLFAKYVRTLSENMENPSFQQYQT
                     WAEKYQLSSEEINGWKNAFITRFFDNPEPTFQFWRDEQIKLLKTDNLTERRLRMAIWQ
                     KADLNEWLVLLSQQAKEKAEWRYWAAKEEKNEAKRKVDFESLAKERGFYAMLAAQQLG
                     IVYQIPMLEVPELTQAQLELYANQLARIQELRELGQFDQARKIWIDWIKNIKPEAGEN
                     TPTKIQLALIKYAKDKNWYDLAVEGTIQTKAFDYLDLRLPNAYSDWFDLHLQDKKISK
                     TFAQAIARQESAWNAQAQSHANARGLMQLLPTTAQKTAKDNGLTFKDGNDLFQPFNNI
                     MLGTTHLMELNEKYPNNRILIASAYNAGAGRVEQWLKRSNGQLAMDEFIASIPFYETR
                     GYVQNVLAYDYYYQMLYSDINDKNGLKMFYQEELTRKY"
     gene            4869..4945
                     /locus_tag="PXD66_000558"
     tRNA            4869..4945
                     /locus_tag="PXD66_000558"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4903..4905,aa:Met,seq:cat)
     gene            5172..5633
                     /gene="rimP"
                     /locus_tag="PXD66_000559"
     CDS             5172..5633
                     /gene="rimP"
                     /locus_tag="PXD66_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDL6318890.1"
                     /translation="MATLEQKLEDLVLDSIEALGFELVGIECQRAGRFLTVRLYIDKE
                     GGVTIDDCSDVSRQVSAIFDVEDPIADKYNLEVSSPGLDRPLFTVAHYERFIGQDVVI
                     HLRIPMFDRRKWQGKIKQVEGDFITLTVENEDRQFAFGNIQKANLVPVFNF"
     gene            5654..7150
                     /gene="nusA"
                     /locus_tag="PXD66_000560"
     CDS             5654..7150
                     /gene="nusA"
                     /locus_tag="PXD66_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDL6318891.1"
                     /translation="MSKEILLAAEAVSNEKQLPKEAIFEALETALTIATKKSRDLDID
                     VRVVVDRKTGDFQTFRRWAVVENVNNMTREISLEAAQFEDPNVQLGDFIEDEVESIEF
                     GRVAIQTFGQVVKTKIREAEKTKIIDEFRSDLGKIILATVKKVTRDNIFLELQGKSED
                     IKGEAVIVREDMIPRENFRPGDRVRGVLYEIKPESKGPQLFVTRAKPVMLEELFKLEV
                     PEIGEGVIEIKGSARDVGSRAKIAVKTHDKRIDPVGACVGMRGARVQAISNELGGERV
                     DIVLWDENPAQFVINAMAPADVSSIVINDETHSMDIAVEAKNLAQAIGRNGQNVRLAT
                     QLTGWALNVMTVEELTKKSEEEDGKVINLLTSALEIDEEFAQVLIEEGFTSLEAIAYV
                     PVDTLTAIDGLEDEDLVEELQSRAKNAITKKALEEEKLLKSAQIEEKLLTLEGMDRHI
                     AFKLAEKQITTLEELAEQGVDDLADIEELSEEKAGELIMAARQICWFN"
     gene            7135..9702
                     /gene="infB"
                     /locus_tag="PXD66_000561"
     CDS             7135..9702
                     /gene="infB"
                     /locus_tag="PXD66_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDL6318892.1"
                     /translation="MLVQLSKEDNKMTEEKKTLSLGGARKASKVNTTTTSGKVKAVEV
                     KEKKPKIDAKALQEKAAKEAAAAKARAEKEAAEKLAKEKAEAEAKEKAAKKAAESTAS
                     KPISVPVMPSSKAKEEKPKAQQVQPKQEKAVDPEKEAKRKEEAELRRKQEELAQQKAE
                     MEAKRAAENARRLAEIAREEAEDNGNEDFDDERFTSSYAREAERDNDRRSEGNRGRNG
                     KGNVAKAKKGGREEDKNERSADRRNHKEVKGKGKQGKKGAALQQAFTKPAQVVKADVV
                     IGETITVAELANKMAVKATEIIKMMMKMGEMVTINQVIDQETAQLVAEELGHKVILRK
                     ENELEEAVLEDRDTHAEKVTRAPVVTIMGHVDHGKTSLLDYIRKAKVAAGEAGGITQH
                     IGAYHVETDDGKMITFLDTPGHAAFTSMRARGAKATDIVVLVVAADDGVMPQTIEAIQ
                     HAKAAGAPLVVAVNKIDKPEANPDRVEQELLQHEVISEKFGGDVQFVPVSAKKGMGID
                     DLLEAIILQSEVLELTAVKDGMASGVVIESYLDKGRGPVATILVQSGTLNKGDIVLCG
                     FEYGRVRAMRDENGKDVESAGPSIPVEVLGLSGVPAAGDEATVVRDEKKAREVALYRQ
                     GKFREVKLARQQKAKLENMFSNMAAGDVAELNIIVKADVQGSVEAISQSLMELSTDEV
                     KVKIVGSGVGGITETDATLAAASNAIMVGFNVRADASARRVIENENIDLRYYSIIYEL
                     LNDVKAAMSGMLQPEFKQEIIGLAEVRDVFRHPKFGAIAGCMVTEGIVKRNNPIRVLR
                     DNVVIFEGELESLRRFKDDVSEVRNGMECGIGVKNYNDVKVGDQIEVFEVVEVKRTI"
     gene            9787..10167
                     /gene="rbfA"
                     /locus_tag="PXD66_000562"
     CDS             9787..10167
                     /gene="rbfA"
                     /locus_tag="PXD66_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDL6318893.1"
                     /translation="MSREFKRSDRVAQELQKEVAVILQREVKDPRIGMVTVSDVEVSR
                     DLAYAKIFVTFLFDNDAEAIKQGMQGLEKASPYIRTLLGKAMRLRIVPELRFIYDESL
                     VEGMRMSNLVSSVIKNDEEKHKED"
     gene            10170..11093
                     /gene="truB"
                     /locus_tag="PXD66_000563"
     CDS             10170..11093
                     /gene="truB"
                     /locus_tag="PXD66_000563"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDL6318894.1"
                     /translation="MSRPRKRGRDIHGVFLLDKPQGMSSNDILQKVKRIFQANKAGHT
                     GALDPLATGMLPICLGEATKFSQFLLDSDKRYLVTAKLGERTDTSDAEGQVVETKAVA
                     VTKSQILAALEKFRGDILQVPTMFSALKHNGKPLYEYARAGITVEREARPITIFELNF
                     IEYKAPFLTLEVHCSKGTYIRTLVDDLGEELGCGAHVTMLRRTAVADYPIEKMMSYAD
                     LQNLAENQPLAELDQHLLPMDTAVSALAKINLSSEQTKAVGFGQRIRFDNLNQLYGLV
                     RLYSEDEQFLGVAEITNDTVIRPNRMINLTE"
     gene            11096..12176
                     /locus_tag="PXD66_000564"
                     /pseudo
     CDS             11096..12176
                     /locus_tag="PXD66_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252783.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
     gene            complement(12214..12738)
                     /gene="tadA"
                     /locus_tag="PXD66_000565"
     CDS             complement(12214..12738)
                     /gene="tadA"
                     /locus_tag="PXD66_000565"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDL6318895.1"
                     /translation="MFIKPNQTSCPLQISEQDIAFMQYALDLADLAEAKGEIPVGAVL
                     VDKNQQIIGRGWNQTIQLCDPSAHAEMQAIRQAGQTLGNYRLLDCTLYVTLEPCPMCA
                     GAILHSRIKRLVFGASDYKTGAVGSRYHLFEDYKMNHFLEVHGNVLGRECSQKISDFF
                     KQRRLQKKGDALIK"
     gene            complement(12820..14628)
                     /locus_tag="PXD66_000566"
     CDS             complement(12820..14628)
                     /locus_tag="PXD66_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL6318896.1"
                     /translation="MTTESLTFADLGLPQPILDAVNEMGFVNPSPIQQECIPHLLAGR
                     DVLGMAQTGSGKTAAFSLPLLAQIDPTQRHPQMLVMAPTRELAIQVADACEQFAKNMK
                     GIRVVTVYGGQRYDIQLRALKQGSQVVVGTPGRILDHIRRGTLDLSELKFIVLDEADE
                     MLRMGFIDDVETVMAELPETHQTALFSATMPEPIRRITRRFMKDPQEVKIKATERSAP
                     DIEQSYWLVNGFRKNDALLRFLEVEEFDAAIIFTRTKTGTIDITELCERNGYRTAALN
                     GDMTQQAREQTLDKLRSGRLDILVATDVAARGIDIERISLVINYDIPLEEESYVHRIG
                     RTGRAGRSGRALLFVEPRERRLLRNIENLIKKPINEVAIPNHEILMEKRREKFKARIS
                     KQLEHHDLEKYRELLEDLFTADQDHEELAAAMMMLLQEKQKLILPPDPVVRAARSERG
                     RDRNERNDRRNGREHRENNGVAMDLYRIELGREDGVEVRHIVGAIANEGDISSRYIGH
                     IKLYDKYSTIELPQGMPNHLVQHFAHKARVLSKPMQMTLLGSANSASNANPFEGRGRR
                     GNDRNDRGGRRDGGFNKDRKGGFKEKRFSEKRGRRD"
     gene            complement(14830..15753)
                     /gene="nlpI"
                     /locus_tag="PXD66_000567"
     CDS             complement(14830..15753)
                     /gene="nlpI"
                     /locus_tag="PXD66_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HDL6318897.1"
                     /translation="MVQLFKLFRIRFLLLNLISVLMLSGCTSRYADLVSIKNLALAEL
                     NPQLRFEQEAMVVRLSQVLEEAPLTPSEQADLYFERGVIYDSLGLWSLARYDFAQAIS
                     LNQRMAAAYNYMGLYLLLEGDYDSSIDAFNAVLELDREYSYTYLNRGLAFYYSGRYSE
                     AQRDLLHFYEEDKKDPYRALWLYFNELEFTPTEAKNNLQKRANNLSSDYWGTNIVNYF
                     LGKLNLAQVRAIMERDAKENSSQYAEILTETYFYLAKQQLKLDHKDEAISLFRLALAN
                     QVFNFVEYRFALFELSQIRGMTQTTPVVDKE"
     gene            complement(15834..>17980)
                     /gene="pnp"
                     /locus_tag="PXD66_000568"
     CDS             complement(15834..>17980)
                     /gene="pnp"
                     /locus_tag="PXD66_000568"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDL6318898.1"
                     /translation="PIVKQFKYGQHTVTLETGAIARQATAAVMASMDDTTVFVTVVAK
                     KDVKEGQDFFPLTVDYQERTYAAGRIPGGFFKREGRPSEGETLIARLIDRPVRPLFPE
                     GFFNEIQVIATVVSVNPQISPDLVAMIGASAALSLSGVPFNGPIGAARVGFINDQFVL
                     NPTTSEQKISRLDLVVSGTDKAVLMVESEADILTEEQMLAAVVFGHEQQQVVIENIKE
                     FVKEAGKPRWDWVAPEPNTDLINKVKALAETRLGDAYRIVEKQVRYEQIDAIKAEVIA
                     QLTAEDETVSEGTIIDIITALESQIVRSRIIAGEPRIDGRTVDTVRALDICTSVLPRT
                     HGSALFTRGETQALAVATLGTERDAQIIDELTGEKSDRFLFHYNFPPYSVGETGRIGS
                     PKRREIGHGRLAKRGVLAVMPTAEEFPYVVRVVSEITESNGSSSMASVCGASLALMDA
                     GVPIKAAVAGIAMGLVKEDEKFVVLSDILGDEDHLGDMDFKVAGTRTGVTALQMDIKI
                     EGITPEIMRIALNQAKGARMHILGVMEQAIPAPRADISDYAPRIHTMKIDPKKIKDVI
                     GKGGATIRALTEETNTSIDIDDDGTVKIAATDGNAAKAVMARIEEIVAEVEVNQIYNG
                     KVTRVVDFGAFVSILGGKEGLVHISQITNERVERVADYLTVGQEVQVKVVEIDRQGRI
                     RLTMKDINNTNEANAEETVAENVVETEQENNF"
BASE COUNT         5476 a         3432 c         4044 g         5028 t
ORIGIN      
        1 ttattgtcgc tgttacgatt ggagccaata atgatcaact tatcaccttt aactatattt
       61 ttgctcagag tgaattacgc ttatcaacgt tagttgcgat tttatttggc ttaggattga
      121 ttcttggttg gttggtaacg ggtatttttt acttaaaaat taagtttcaa aacgttgctt
      181 taaatcgtcg tgtaaagcgc caagctcagc aaataactga attaacgact ccaaaggctg
      241 aataatcaat gcttgaatta ttgttccttt tattgcctat tgcagcacta tatggttggt
      301 atatgggaca acgtagtgca aagaaggatc aagaaaccgc tagtaataag ttctctcgtg
      361 agtatgttac ggggttaaat tttcttttat ctaatcagca ggaaaaggcg gtggaccttt
      421 tcctctctat gttacaaaaa caagaatccg aaaatcatat tgcatctgaa tcccaatttg
      481 aagcggagtt aaccctaggt aatcttttcc gttcaagagg tgaggtagat cgtgccttac
      541 gtattcacca aacattagtg aatagccctc attattcatt tgatcaaaaa ttattagcaa
      601 aacagcaatt agctaaagac tttatggttg caggtttcta tgaccgtgca gaaaattatt
      661 acatttcatt agttgatgaa cctgagtttg ctaaatattc tttatctcag cttgctgtta
      721 tttaccaaaa aaccagagaa tggaaaaaag cgattaatgt tacagaaaaa cggcttcgaa
      781 tagagccgga tgcagataaa ataccgcttt cacattttta ttgtgaatat gcgcaagggg
      841 ttaaatttga tgataaagcg gcatttattg aatcgttaaa taaagcgtta gaatattatc
      901 ctcaatccac acgcgcttct ataatattag gtgatttcta tttagaagag ggagaatatc
      961 taagagcatt aagctatttc gaacagattc ttgtgcaaga ccctgattat attagcgaag
     1021 tgcttggacg aattaagcaa agctatatgg ctcttgagga cttaaatggc tatgagttat
     1081 ttctcattcg agcaaaccga gtaaaacata atagtagtgt agatattgct ttaactgaat
     1141 ttattgaaga aaaagatggt atcaatgctg cacattctaa actataccag cagttgagta
     1201 cttatccgaa tttaattact tttcaccgct ttattcgcta tcaagcagat tttgcggagg
     1261 aaggaaatgg taaggaaagc ttagttttat tacataatat ggtgggaaat cagataaaac
     1321 gcagcttcca atatcgctgt ttaaattgtg gttatcaaag ttatcgctta atgtggcaat
     1381 gtccttcttg taatcaatgg gaaaaaatta agccggtaca aagtatcgaa ggtattattc
     1441 aataaagaaa gaatctgagg aattgaaaat gaacaataaa gtaattgttg cgttagacta
     1501 tgaaactgaa cgtgaagcat tagaatttgt ggatttggtc gatccgtcgc tttgtcgttt
     1561 aaaagtaggc aaagaaatgt ttacaacctt aggcacaaat tttgtcaaac aattacacga
     1621 acgtaaattt gatgtattcc ttgatttaaa atatcatgat attccaaata cggttgctcg
     1681 agcagtacgt tctgctgctg atcttggtgt gtggatggtt gacttgcatg cttctggtgg
     1741 tttagccatg atggaagagg caaagaaaat tttggagcct tatggcaaag aggcaccatt
     1801 attaattgca gtgacggttc taaccagtat ggaagatttg gatttgcttc aaataggtat
     1861 taatgcttcg ccgatggaac aagttattcg tttagcacat ttagcacaac gggctggttt
     1921 agatggcgtg gtatgttctc cacaagaagt tgaggtatta cgtacccatt gtggtcaaga
     1981 ttttaaatta gtgaccccag gaatccggcc aaaaggaaca gattttgggg atcaacgccg
     2041 agtgatgaca cccaaacaag cagtagaaac aggctcagat tttttggtta ttggtcgtcc
     2101 tattacccaa gctcaagatc cgcttgcggt attaaaatct attaaccaat ctatcagtta
     2161 attcattgca tttttaatga aggcggaaaa atttccgcct tttttaatca aacaagcggt
     2221 tatttttgag gaaaaatttg taaatgacat tagtctattc aacggaaaca ggcagaataa
     2281 agtccgaagt aataaaagaa gaacgcccta aaggggatgg tattgtgcgt attcaacgtc
     2341 aaacaagcgg tagaaagggg aaaggtgttt gccttatcac cggtcttgat ttagaggata
     2401 aagccttaaa ttctcttgct tcagagctta aacgtaaatt gggttgtggt ggctcattta
     2461 aagatggagt aattgaaatc cagggcgata atcgaggatt cattaagcaa atcttagaac
     2521 agaaaggttt caaagtgaaa ctggcaggtg gttgagtttt aaacaattat cagctggcag
     2581 tttttaaata acttcatttt atttgaaact tcattcctac ttttctgcct aaaactcacg
     2641 attaaggaga aaaactaatg tggaagaaaa cgttgattgc tgtattttta gcaagccaat
     2701 atgcttttgc ggtaggaacc gggaaagaac catcaaaaag ttatactgaa acagaattaa
     2761 aacaattaaa aaatgattgg ttagcgattt atcaaaaaga gctacagtat gcagaaaatt
     2821 tgcgtttaaa acagagagaa aattttcagc aaattaatta tttggcagat gcagcgatta
     2881 gtcagaagaa tttatcactt tcaacggagc agataattca gcgtttatta ctaaccttag
     2941 cgggttatcc gttggagatg aaagctgaat ggaagctgct aaatgctaaa ataacgttaa
     3001 aacaagccac agaaagtgaa gttaaagcat ttatttcaaa atatcctgat tctttgtatc
     3061 aaaggcagtt acagaacttg atgtttgagc tgctctatcg tgaacagaaa tttccggagt
     3121 tactggaata tgctcaaaat gtaaaacaga gtagtcttgc cgaccaatgc cgtttatttt
     3181 ctgctcgtta tgaactggca gcaagtaaag ctcaaatcaa tccggaagtt tctcaagtaa
     3241 ctgagcaaaa agcagaaaat gcagaactgc ttgctttggt gcaagagttt gatcgctttt
     3301 ggcttgctac gccgaaatta acttctgatt gtgcgaattt agaaagttat tggcgggatc
     3361 aggggcttaa aaccgatgaa aaaatgcgtt tgaaagcggt cgaattaatt aaacaaaatg
     3421 caaatgcaga attggcgaat ttggcggcaa atgtaaataa tgagaatttg aaaatctggc
     3481 tatcttccgt tgaaaaggta gcgaataatc cggcagattt gcaaaatttt atcgaaaatc
     3541 aaccgcttga ttccccattt tatgctgata ataaagcgat tattcagcaa ctctttgcta
     3601 aatatgtgcg aactttatcg gaaaatatgg agaacccgag tttccagcag tatcaaacgt
     3661 gggcagagaa gtatcaactt tccagcgagg aaatcaatgg ctggaaaaat gcttttatca
     3721 ctcgattttt tgataaccca gaaccaacat tccaattttg gcgtgatgag caaatcaagt
     3781 tgctcaagac agacaattta actgaacgcc gtttacgaat ggcaatttgg caaaaagcag
     3841 atttaaatga gtggctggtt ctgctatctc agcaggcgaa agaaaaagca gaatggcgtt
     3901 attgggctgc gaaagaggag aaaaacgaag cgaagcgtaa agttgatttt gaaagcttag
     3961 ctaaagagag aggcttttat gctatgttgg ctgctcaaca attaggcatt gtttaccaaa
     4021 tcccgatgct tgaagtgcct gaattgacac aagcacagct tgaattgtat gcaaaccaat
     4081 tagctcgtat tcaagagttg cgagagctag ggcagtttga ccaggcaaga aaaatatgga
     4141 ttgactggat aaaaaacatc aagccggaag caggtgaaaa tacaccaact aaaatccagt
     4201 tagccctgat aaaatatgcc aaggataaaa attggtatga tttggcggtg gaaggtacaa
     4261 ttcaaacaaa agcctttgat tatcttgatt tgcgattacc gaatgcctat tcggattggt
     4321 tcgatttaca tctgcaagat aaaaaaatca gcaaaacgtt tgctcaggcg attgctcgtc
     4381 aagaaagtgc gtggaatgct caggctcaat cgcacgctaa tgcgagaggg ctaatgcaac
     4441 tgttaccgac aacagcacaa aaaaccgcta aagataacgg tttaaccttc aaagatggca
     4501 atgatctctt tcaaccgttt aacaatatta tgttaggcac aacccattta atggagttaa
     4561 acgaaaaata tccgaataat cggattctga ttgcctctgc ttataatgca ggagcagggc
     4621 gtgttgagca gtggctgaaa cgtagtaacg ggcagttagc aatggatgaa tttattgcca
     4681 gtatcccgtt ttatgaaacc agaggctatg tacaaaatgt gctggcgtat gactactatt
     4741 atcaaatgct ctatagcgat ataaatgata aaaatggatt aaaaatgttc tatcaagagg
     4801 aattaacaag aaaatattaa aaaatgcttg tgtttttgtg tagaaccgct ataatgcacc
     4861 ccatcagacg cggggtggag cagcttggta gctcgtcggg ctcataaccc gaaggtcgtt
     4921 ggttcaaatc cggcccccgc aaccagtttt aagctcacag ctttggttaa agttgtgagt
     4981 tttgttttat atgctgtagc aaatttgcaa aaaatttggc aaaaaagacc gcttgttgtg
     5041 gttgctacag caaaaaacaa accgcttaaa gccccacggt ttcccgtctg gggctttttt
     5101 gcggaaattg gtacagcatt aacgtatcaa agctggttta tccggctttt tttatatttg
     5161 gagattatga cttggcaact ttagaacaaa aattagaaga tttagtgctc gattctattg
     5221 aagcattagg ctttgaatta gtcggcattg aatgccaacg tgcaggccgt tttttaacgg
     5281 tgcgtttata tattgataaa gaaggtggcg tgacgattga tgattgtagc gatgtcagcc
     5341 gccaagtgag tgcgattttt gatgtggaag atccgattgc cgataaatat aacttagagg
     5401 tttcttcacc agggttagat cgtccattat ttaccgttgc ccactatgag cgttttatcg
     5461 ggcaagatgt agtgattcat ttacgcattc cgatgttcga tcgccgtaaa tggcaaggta
     5521 aaatcaaaca agtagaaggt gatttcatca ctttaacggt agaaaatgaa gatcgccagt
     5581 tcgcatttgg caatattcaa aaagcaaatt tagttccggt ttttaatttt taagataaag
     5641 gtaaactgac aaaatgagta aagagatttt attagctgcg gaagcagttt caaacgaaaa
     5701 gcaattacca aaagaggcaa tttttgaagc cttagaaacc gctttaacca ttgcaaccaa
     5761 aaagagccgt gatttagata ttgacgtgcg tgtggtggta gatcgtaaaa ccggtgattt
     5821 ccaaactttc cgtcgttggg ctgtggttga aaatgtgaat aatatgaccc gtgaaatcag
     5881 cttagaagcg gcacaatttg aagatccaaa cgtacaatta ggcgatttta ttgaagatga
     5941 agtagagtct atcgagttcg gtcgtgtggc gattcagact tttggtcagg tagtcaaaac
     6001 caaaattcgt gaagcggaga agacgaaaat cattgatgaa ttccgttcgg atttaggcaa
     6061 aattattctt gccactgtga aaaaagtaac ccgtgataat attttccttg agttacaagg
     6121 taaatcagaa gatatcaaag gcgaagcggt cattgtgcgt gaagatatga tcccacgtga
     6181 aaacttccgc ccgggtgacc gtgtgcgtgg tgtgttatac gaaatcaagc ctgaatcaaa
     6241 aggacctcag cttttcgtta cccgtgctaa accggtgatg ttagaagagc tattcaaatt
     6301 ggaagtgcct gaaattggcg aaggcgtaat tgagatcaaa ggttcagccc gtgatgtcgg
     6361 ctctcgtgca aaaattgccg tgaaaaccca cgataaacgt attgacccgg taggggcttg
     6421 tgtgggtatg cgtggtgctc gtgttcaagc tatcagcaat gaattaggcg gcgagcgtgt
     6481 ggatatcgta ctttgggacg aaaatccggc tcaatttgtg attaatgcaa tggctccggc
     6541 agatgtaagc tctattgtga ttaacgatga aactcactca atggatattg cggttgaagc
     6601 gaaaaatctg gctcaagcta tcggtcgtaa cggtcaaaac gtgcgtttgg cgacacaatt
     6661 aactggttgg gcattaaatg taatgaccgt tgaagagcta acgaagaaga gcgaagaaga
     6721 agatggtaaa gtcatcaatt tattaacttc tgctttagaa attgatgaag aatttgctca
     6781 agttttaatt gaagagggct ttacgtctct tgaagcgatt gcttatgtgc cggttgatac
     6841 tttaacggcg attgacggtt tagaagatga agatttagtc gaagaattac aatctcgtgc
     6901 caaaaatgcg atcacgaaaa aagcgttaga agaagagaag ttattaaaat cagctcaaat
     6961 tgaagaaaaa ttattaacgc ttgaaggtat ggatcgccat atcgcattca aattagctga
     7021 aaaacagatt acgactcttg aagaacttgc agagcaaggt gttgatgatt tagccgatat
     7081 tgaagaactt tcagaagaga aagctggcga gctaattatg gcagcacgcc aaatttgttg
     7141 gttcaactaa gcaaagagga taataaaatg acagaagaga aaaaaacact ttcgcttggt
     7201 ggcgctcgca aagcgagtaa agtgaataca accacaacca gtggtaaagt aaaagcggtt
     7261 gaagtaaaag agaaaaaacc gaaaattgat gcaaaagcat tgcaagaaaa agcagcaaaa
     7321 gaagctgccg cagcgaaagc tagagctgaa aaagaagcag ctgaaaagtt agccaaagaa
     7381 aaagccgaag ctgaagctaa agagaaagct gcaaaaaaag cagcagaatc gaccgctagt
     7441 aaaccgattt ctgtgccggt aatgccaagc agcaaagcaa aagaagaaaa accgaaagca
     7501 caacaggtac agccaaaaca agagaaagcg gttgatcctg aaaaagaagc taaacgcaaa
     7561 gaagaagctg agttacgccg taagcaagag gaattagctc agcaaaaagc ggaaatggag
     7621 gcaaaacgtg ctgcagaaaa tgcccgccgt ttagcagaaa ttgctcgtga agaagcggaa
     7681 gataacggca acgaagattt tgatgatgag cgttttactt cttcttatgc tagagaggct
     7741 gagcgtgata atgaccgccg tagtgaggga aatcgtggtc gtaacggcaa aggcaatgtg
     7801 gctaaagcga aaaaaggcgg acgtgaagag gataaaaacg aacgcagtgc agatcgtcgc
     7861 aaccataaag aagtaaaagg caaaggtaaa caaggtaaga aaggtgcagc tttacaacaa
     7921 gcctttacca aaccagctca agttgttaaa gctgatgtgg tgattggtga aactatcact
     7981 gtggctgaat tggctaataa aatggcagtg aaagcgactg aaatcatcaa aatgatgatg
     8041 aaaatgggtg aaatggtgac gatcaaccaa gttatcgacc aagaaaccgc acaattagtg
     8101 gcggaagaac ttgggcataa agtgattttg cgtaaagaaa atgagttaga agaagcggtt
     8161 ttagaagatc gtgatactca cgcagaaaaa gtaacccgtg caccggtagt gactattatg
     8221 ggccacgttg accacggtaa aacctcgtta cttgactaca ttcgtaaagc caaagtggcg
     8281 gcaggtgaag caggtggtat tacccagcac attggtgcat accacgttga aaccgatgac
     8341 ggcaagatga tcactttctt agataccccg gggcatgcag cctttacctc aatgcgtgcg
     8401 cgtggtgcga aagcaactga catcgttgtt ctcgtcgttg cggctgatga cggtgtaatg
     8461 ccacaaacta ttgaagctat ccagcacgcc aaagcagcgg gtgcgccatt agtggtagcg
     8521 gtgaataaaa ttgataaacc ggaagcgaac cctgaccgtg tagaacaaga gttactacaa
     8581 cacgaggtta tttctgagaa attcggtggc gatgtgcaat tcgtgccggt atctgcgaaa
     8641 aaaggtatgg ggatcgatga tttactagaa gccattattc tgcaatccga agtgcttgaa
     8701 ttaaccgcag taaaagacgg tatggcaagc ggcgtggtaa tcgaatctta cttagataaa
     8761 ggtcgtggcc ctgtggcaac tatcttggtt caatccggta cgctaaacaa aggcgacatt
     8821 gttctatgtg gttttgaata cggacgtgtg cgtgcaatgc gtgatgaaaa cggtaaagat
     8881 gttgaatcag caggtccgtc tattccggta gaagtattag gcttatcagg cgtgcctgct
     8941 gccggtgatg aggcaaccgt cgtgcgtgat gagaagaaag cccgagaagt ggctctttat
     9001 cgccaaggta aattccgtga agtaaaactg gctcgtcagc aaaaagctaa actggaaaat
     9061 atgttcagta atatggcagc cggtgatgtg gcagaattga acatcattgt taaagcagac
     9121 gtacaaggct cggtagaagc gatcagccaa tccttaatgg aactttcaac cgatgaggtg
     9181 aaagtgaaaa ttgttggttc aggtgtaggt ggtattaccg aaactgatgc aaccttagcc
     9241 gcagcttcta atgctattat ggtcggcttt aacgtgcgtg ccgatgcctc tgcccgccgt
     9301 gtgattgaaa acgaaaatat cgatttacgt tactactcca tcatttatga gctattaaat
     9361 gacgtgaaag cagcaatgag cggtatgtta cagcctgaat tcaaacaaga aattatcggc
     9421 ttggcagaag tgcgcgatgt gttccgccac ccgaaatttg gggcgattgc cggttgtatg
     9481 gtgacagaag gtatcgttaa acgtaacaac ccaatccgtg ttctgcgtga taacgtagta
     9541 atttttgaag gcgagttaga atctctccgc cgtttcaaag atgacgtatc tgaggtgcgt
     9601 aacggtatgg aatgtggtat cggcgttaaa aactacaacg acgtaaaagt tggcgaccaa
     9661 atcgaagtgt ttgaagtggt tgaagtaaaa cgtactattt agcctaattt tgtgtagggg
     9721 cgtattgcat acgccctttg tattttttag ggcgtgaagc atcacgcccc tataaaagag
     9781 aaaaatatgt ccagagaatt taaacgtagt gaccgtgtgg cacaggagct acaaaaagaa
     9841 gtggcggtga ttttacaacg tgaggtaaaa gatcctcgca ttggtatggt gactgtttct
     9901 gatgtggaag tatcaagaga tttggcgtat gccaaaattt ttgtgacttt cttatttgat
     9961 aacgatgctg aagctattaa gcaaggtatg caaggcttgg agaaagcaag cccctatatt
    10021 cgaacccttt taggtaaagc aatgcgtttg cgtattgtgc ctgaactgcg ttttatctac
    10081 gatgaatcgc tagtcgaagg tatgcgtatg tctaaccttg tgtctagtgt aatcaaaaat
    10141 gacgaagaaa aacataaaga ggactaattg tgtctagacc tcgcaaacgt ggccgtgata
    10201 ttcacggcgt tttcttatta gataaaccac aaggaatgtc ttcaaacgat attctgcaaa
    10261 aggtaaagcg aattttccaa gcaaataaag ccggacatac cggggcgtta gaccctctcg
    10321 caaccggaat gctgccgatc tgtttaggtg aagcgaccaa attttcgcaa tttttgttgg
    10381 attcggataa acgttatctc gtcaccgcaa aattaggcga gcgaaccgat acttcagatg
    10441 cggaagggca agtggtggaa accaaagcag ttgcagtgac aaaatctcaa attttggctg
    10501 ctttggagaa atttagaggt gatattttgc aagtgccgac aatgttttcg gccttaaaac
    10561 acaacggcaa acccttgtat gaatatgccc gagcagggat tacggtggag cgtgaggctc
    10621 gcccgattac gatttttgaa ctaaatttca ttgaatacaa ggctccattt ttaacccttg
    10681 aggtgcattg ttctaaaggt acttatatcc gaactttggt cgatgattta ggcgaagagt
    10741 tgggctgtgg tgcacacgtt acgatgctac gccgtacagc agtggcagat tatccgattg
    10801 aaaaaatgat gagctatgca gatttgcaaa atttagccga aaatcaaccg cttgcagagt
    10861 tagaccagca cttattgccg atggatacgg cagtttctgc attagcaaaa ataaatttaa
    10921 gttcagagca aactaaagcg gtgggttttg gtcagagaat tagatttgat aatctgaacc
    10981 aactttatgg tttggtacgt ttatattcag aagatgaaca atttctcggt gtggcagaaa
    11041 tcaccaatga tactgtgatt cgtcctaacc gaatgattaa cttaacggaa taagaatgaa
    11101 atcgataatt tttaccttac ctttagcgat tatcacttta gccttagccg gttgttcaag
    11161 tgataatcaa gaaagttatg ccggcttaaa cttagatgct aaatacagta aatctcgctc
    11221 ggtcagtcat tttaatgatt atgtgcattt tctgaaacag aaggcagcag gggcaggagt
    11281 gtctgcaaaa accttaaatg ctcagaaatt cattcattac aacgccagag cggtgcagtt
    11341 agatcaacag caagctgcac gaagacgtga cccgaatgta ccgcctccgc caccaaatcc
    11401 aagttttaaa caaagtatta actcaagcga aggtggatca ggcaagtgaa cgttggtatg
    11461 aatataatag ccagcttacc catgccagcc aaaaatatgg ggtacaaaaa gagtatattt
    11521 tggcactttg gggaatggag agcagctttg ggcgtaacca aggggatttt gatgtacttt
    11581 cggtgttggc aaccctcgcc tttgatggtc gccgtgagaa gttatttacg caagaatttg
    11641 tgaatgcgat gaaaatgctc gatgacggca caattaaacg ctatgaattg cttggatctt
    11701 gggcaggagc gatgggacaa actcaattta tgccaacttc ttacttaacc tatgcagctg
    11761 atggcaataa tgacggtaaa aaagatattt gggagaatga agccgatgca tttgcctcga
    11821 ttgccaatta tctttcaaca gtaggttggg ataatcaact accttggggt gttgaggtta
    11881 aattagcttc accgatggat ttaagtttcg ccggtcttga aaccaacaaa gcgaaaacat
    11941 tggcggaatg gcaagcctta ggtatttatt tagcttatcc gaatggacaa gaatcggcaa
    12001 aattagctca attacacggc aaagaggttt ggttgattcg tccggataaa gaagcgggaa
    12061 gagcgttttt agtttctaat aatttccgta ctttaatgga ttggaataaa tcgaataacc
    12121 ttggtatcag tatcggaaaa tttgccgatc gaattttagc ttcagttggt caataatcga
    12181 ataaaagcat agtagcaaag cctatctatg cttttatttt atcaaagcgt cgcctttctt
    12241 ctgcaaacga cgttgcttaa aaaaatcact gatcttttgg ctacattctc tgcccaatac
    12301 attgccatgc acttctaaga aatggttcat tttataatct tcaaacagat gatagcgtga
    12361 acctaccgca cctgttttat aatcacttgc cccaaaaacc aatcgcttaa tccgactatg
    12421 taaaattgca ccggcacaca ttgggcaagg ctcgagcgta acataaaggg tacaatctaa
    12481 cagacggtaa ttccctaaag tttgtcctgc ttgacgaatt gcctgcattt cagcgtgtgc
    12541 agatggatca cagagttgaa tggtttggtt ccagcctctg ccgataatct gttggttttt
    12601 atcaaccagt actgccccaa ccgggatttc gccttttgct tcggctaaat cagcgagatc
    12661 aagggcgtat tgcataaagg cgatgtcttg ctcggagatc tgaagtggac aagaagtttg
    12721 atttggtttg ataaacatag ttgcaaaaaa ttgagttttt ttgaccgctt gtaaaatgcc
    12781 ccacgagggt ggggcatcgc ttacttcgct tagtgtcgtt tagtcacgac gaccacgttt
    12841 ttcactgaaa cgtttttctt taaagccacc tttgcggtct ttattgaaac ccccgtctcg
    12901 gcgaccacca cgatcattac gatcgttacc acggcgacca cgaccttcaa atggatttgc
    12961 attacttgca ctgtttgcag agccaagtaa tgtcatctgc attggtttgc ttaatacacg
    13021 tgctttgtga gcgaaatgtt gtacgagatg atttggcatt ccttgcggca actcaatggt
    13081 tgagtattta tcataaagtt tgatatgccc gatgtaacgg ctgctgatat ccccctcgtt
    13141 ggcaatcgct ccaacaatat gacgaacttc cacaccgtct tcacgcccca attcaatgcg
    13201 gtataaatcc attgccacac cattgttttc acggtgttca cgaccgttac gacgatcgtt
    13261 tcgctcattg cggtcacgtc cacgttcaga acgggcagca cgcacaaccg gatccggtgg
    13321 cagaatcagt ttttgttttt cttgcagtaa catcatcatc gccgctgcta attcttcgtg
    13381 gtcttgatcc gcagtaaata aatcttccaa caattcacga tatttttcta aatcgtgatg
    13441 ttctaactgc ttagaaatgc gggctttgaa tttctcacgg cgtttttcca ttaaaatttc
    13501 gtggtttgga atcgccactt cattaattgg ttttttgatt aaattttcga tattgcgtaa
    13561 taagcggcgt tcacgaggct caacaaacag taaggcacga cctgaacgac cggcacgacc
    13621 ggtacgtccg atgcggtgaa cataagattc ttcttctaac ggaatatcgt agttaataac
    13681 caagctgatt cgctcaatat caataccacg ggcagcaaca tcggtggcga ctaaaatatc
    13741 taaacgaccg gagcgtaatt tatctaaggt ttgctcacgg gcttgctggg tcatatcgcc
    13801 gtttaatgcc gcagtacggt agccgttgcg ttcacacaat tcggtaatat caatagtacc
    13861 ggttttggtg cgggtaaaga tgatagcagc atcaaattct tctacttcta ggaaacgcaa
    13921 taacgcatcg tttttacgga agccgtttac cagccagtag ctttgttcaa tatccggggc
    13981 tgaacgctcg gttgccttaa tttttacctc ttgcggatct ttcataaaac gacgggtaat
    14041 acggcgaatc ggctccggca tggtggcaga gaagagagca gtttgatgtg tttccggtaa
    14101 ttcagccatt accgtttcaa catcgtcaat aaagcccata cgcagcattt catcggcttc
    14161 atctaacacg atgaatttta attcggataa atctaatgtg ccacggcgga tatggtcaag
    14221 aatacgccca ggtgtaccca ctactacttg tgagccttgt tttaaagcac gtaattgaat
    14281 gtcgtaacgt tgtccaccat aaaccgtcac tacacgaatg cctttcatat tttttgcaaa
    14341 ctgctcacaa gcatcggcta cttgaatcgc aagctcacgg gtgggtgcca tcaccaacat
    14401 ttgcgggtgg cgttgtgttg ggtcaatttg tgccaacagc ggcagcgaga atgcggcggt
    14461 tttaccgctc ccggtttgtg ccataccaag cacatcacga ccggctaata aatgaggaat
    14521 acattcttgt tgaatgggtg atgggtttac aaagcccatt tcatttacag catcaaggat
    14581 tggttgaggt aagcctaaat cggcgaaagt taaagattca gttgtcataa aaaaccattt
    14641 gtttggaacg cacaaagcag aaaatatcga ctatttttct gcacagccat taatgaaaat
    14701 tatattgttg aaatttagga agaaatgaaa taacaaagca agtgatgctt taaaaatccc
    14761 ctgagtataa gcggttaaat ttacaaaatt ttttgcgaaa ttaaccgctt gtattgtcct
    14821 aatctttccc tattctttat caacaacggg agtcgtttga gtcataccac gaatttgtga
    14881 taactcaaac agggcaaaac gatactcaac gaagttaaac acttgatttg caagagcaag
    14941 acgaaagagg ctgattgcct catctttatg atctaatttg agttgttgtt ttgctaagta
    15001 gaaatacgtt tctgtcagta tttctgcata ttgtgagcta ttttccttag cgtcacgttc
    15061 cattatagca cgaacttgag ctaaattgag cttccctaaa aaataattta cgatatttgt
    15121 tccccagtaa tccgaagaaa gattattagc tcgcttttgt aaattatttt tagcttctgt
    15181 tggtgtaaat tccagctcat taaaatagag ccataatgct cgataaggat ctttcttatc
    15241 ttcttcataa aaatggagaa gatcacgctg ggcttcagaa tatctgccgc tataatagaa
    15301 agccaaacct cgattgagat aggtgtagct atattcccga tctaattcca atacggcatt
    15361 aaacgcatca atcgaactgt cgtaatcgcc ttccagcagc agatacagcc ccatatagtt
    15421 ataagctgcc gccatacgtt gattgagcga aatggcttga gcaaaatcat aacgggctaa
    15481 cgaccataaa cctaaactgt cgtaaataac gcctctttca aaatagaggt ctgcttgctc
    15541 agagggcgtt aatggggctt cttccaatac ttggctgagt cttaccacca ttgcctcttg
    15601 ttcaaagcga agttgaggat taagttctgc caatgctaag tttttaattg aaaccaaatc
    15661 ggcataccga ctggtacagc cgcttagcat taaaacagaa attaaattca gtaataagaa
    15721 acggattcga aacaacttaa aaagttgtac cattacagca tatccctcaa ttaaggaaaa
    15781 ttaaaaactg aataacggac actcaaagtc cgttattcgc ctttattatt gaactagaaa
    15841 ttattttctt gttctgtttc taccacattt tcagctacag tttcttctgc atttgcctcg
    15901 ttggtattat tgatgtcttt catcgtcaga cgaatgcgtc cttgacggtc aatttctacc
    15961 actttcactt gtacttcttg accaacggtt aagtagtccg ctacacgctc aacacgttcg
    16021 ttggtgattt gtgaaatgtg gactaaacct tctttgccac ctaagatgga aacgaatgca
    16081 ccgaagtcca ccacacgggt tactttaccg ttgtagattt ggtttacttc cacttcggca
    16141 acgatctctt caatacgagc cattactgct ttcgctgcat tgccgtcagt tgccgcaatt
    16201 ttcaccgtac cgtcatcatc aatgtcgata gaagtattgg tctcttcggt taaagcacga
    16261 attgttgcac cgcctttacc aatcacatct ttgattttct tcggatcgat cttcattgtg
    16321 tgaatgcgtg gggcatagtc agaaatatct gcacgaggtg ccggaatggc ttgttccatt
    16381 acacctaaaa tgtgcattct tgcaccttta gcttggttta aggcaatacg cataatttca
    16441 ggggtgatcc cttcgatttt gatgtccatt tgcagcgcag tcacaccggt acgggttccc
    16501 gctactttaa agtccatatc gcctaagtgg tcttcatcac ctaagatgtc agaaagtacc
    16561 acgaatttct cgtcttcttt cacgagcccc attgcgatac cggcaaccgc tgctttgatt
    16621 ggcacacctg cgtccatcaa cgcaagagac gcaccacaca cagatgccat tgaagaagaa
    16681 ccgttagatt cagtgatttc agacaccaca cgcactacat acgggaactc ttcagcggtt
    16741 ggcatcacgg ctaatacgcc acgttttgct aaacgaccgt gaccgatttc acggcgtttt
    16801 ggcgagccga tacgaccggt ttcgcccaca gagtatggag ggaaattgta gtggaataag
    16861 aaacggtcag atttttcgcc ggtcaattcg tcaatgattt gggcatcacg ctctgtgccc
    16921 aatgttgcta ctgctaatgc ttgggtttcg ccacgggtga aaagagcaga accgtgggtg
    16981 cgtggtaaca cactggtgca aatatccaat gcacgcacgg tatccaccgt acggccgtca
    17041 atgcgtggtt cgcctgcaat aatacggcta cgcacgattt ggctctctaa tgcggtgatg
    17101 atgtcgatga tagtcccttc agaaacagtt tcatcttctg cggtaagttg tgcaatcacc
    17161 tctgctttaa tcgcatcgat ttgctcgtaa cgaacttgtt tttctacgat acgataagca
    17221 tcgccaaggc gtgtttctgc taatgctttt actttgttga ttaaatctgt atttggctct
    17281 ggtgcaaccc aatcccaacg tggtttgccc gcttctttaa caaattcttt gatgttttcg
    17341 attacaacct gttgttgctc gtggccgaac accaccgccg ctaacatttg ctcttcggtt
    17401 aagatatccg cttcagattc caccatcaac acggctttgt ctgtacctga aaccactaaa
    17461 tctaagcggc tgattttttg ctcgctggtg gttgggttta atacgaattg atcgttgata
    17521 aaaccgacac gagccgcacc gattggaccg ttaaacggca cgccggataa tgaaagggca
    17581 gccgatgcac cgatcatcgc aaccagatca ggactgattt gtgggtttac cgatactacg
    17641 gtcgcaatca cttgaatttc gttaaagaaa ccttctggga aaagtggacg cacaggacgg
    17701 tcgatcaagc gagcgattaa ggtttcacct tcgctaggac gtccttcacg tttgaagaaa
    17761 ccgcccggaa tacgaccggc tgcgtaagta cgctcttgat aatctacggt taatgggaag
    17821 aaatcttgcc cttcttttac gtcttttttc gctactacgg taacaaatac ggttgtgtcg
    17881 tccatacttg ccattactgc tgccgttgct tggcgtgcga tagcaccggt ttctaaggtc
    17941 acggtgtgct gaccgtattt aaactgtttt acaattggag
//
LOCUS       DANLXF010000028        17765 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.028, whole genome shotgun sequence.
ACCESSION   DANLXF010000028 DANLXF010000000
VERSION     DANLXF010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 17765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17765
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.028"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(54..725)
                     /locus_tag="PXD66_000569"
     CDS             complement(54..725)
                     /locus_tag="PXD66_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL6318899.1"
                     /translation="MLQAIIFDMDGVIVDTEYLEFSLQKQFIEDIKEHSRPITLEQQS
                     EVVGKCLKEIPVIIKKLSESSLPIEEIRARYYAFFQDLFSKVDFKTIFRADIQQIIQF
                     AKQNQIKLAVASSSALSHIENILTVCGIKDEFDLIVSGEQFEHSKPDPTIYRYTCEKL
                     GVEPQNAVAIEDSYYGMLAAKTAGLTVIGYEEKRILIDQSLADYMGKDMKEILKILQK
                     CNTPS"
     gene            complement(846..2309)
                     /gene="guaB"
                     /locus_tag="PXD66_000570"
     CDS             complement(846..2309)
                     /gene="guaB"
                     /locus_tag="PXD66_000570"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005725353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDL6318900.1"
                     /translation="MLRIKQEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTETKLAISLAQEGGIGFIHKNMSIERQADRVRKVKKFESGIVSEPVTISP
                     DMTLAELAELVKKNGFAGYPVIDENQNLVGIITGRDTRFVTDLSKTVREFMTPKDRLV
                     TVKENASREEIFHLMHEHRVEKVLVVNNEFQLKGMITLKDYQKAESKPNACKDEFGRL
                     RVGAAVGAGPGNEERIDALVKAGVDVLLIDSSHGHSEGVLQRVRETRAKYPDLPIVAG
                     NIATAEGAIALADAGASAVKVGIGPGSICTTRIVTGVGVPQITAIAEAAAALKERGIP
                     VIADGGIRYSGDISKAIAAGASCVMVGSMFAGTEEAPGEIELYQGRAFKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRIAYKGYLKEIIHQQMGGLRSCMGLTGCATI
                     EELRTKAEFVRISGAGIKESHVHDVTITKEAPNYRMG"
     gene            complement(2537..5704)
                     /locus_tag="PXD66_000571"
     CDS             complement(2537..5704)
                     /locus_tag="PXD66_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HDL6318901.1"
                     /translation="MKFTDIFIKRPVLAVCISLLITILGLQAIEKLQVREYPEMTVSI
                     VTVSVNYSGADARLMQTLVTSQLEEAVAQADNIDYMTSSSSPSTTTVTAKMKLNTDPN
                     AALSDISAKVNAVRSNLPSGIDDPSIRVSTGSNDALMYIRFVSDELNTSQVTDYINRV
                     VKPQFFTVNGVSSVDVYGSVFGLRIWLDPDKMASNNLSGAAVLSALSANNLQTAAGNS
                     NGYYTVYRNKVLSTTPSVTELEEVTVSTTKDGKTIKLKDIATVELDKYQDAARATVGG
                     KDAVVLAVSVAATANSLTVAKDIYPMFEQVLKNLPSTIQGDIVYDKTIAIDSSIKEVI
                     KTIGEATVIVLVVIILFLGSLRAMIVPVITIPISLIGVLFFLQMFGFSINLLTLLALV
                     LAIGLVVDDAIVVLENVERHVKEGKTPFDAAIIGTREIALPVISMTITLGAVYSPMAL
                     MTGVTGTLFKEFALTLAGAVFISGIAALTLSPMMSSRILKEHKEGEESAFARVVHRSL
                     DKMTSCYTNMLSGIMAVRGFVLFFAVLIFASLPMLFGSLSSEVAPTEDRGFVVGISNG
                     PSNTNLDYTEAALAQFNQEVTKLPEVSSLMTVSGFNGANSALSIIALKDWNDRSRERA
                     AISNDVAAIGKKVVGMDVNAIAFPEISTGENGLPFSLVITTADSYEKLAIVASEFLKK
                     AQTSGQFFFANLDLKFDTVTMNMKFDREKMGAYGVTMQQVSGTLGAFLSGAIITRVDI
                     DSRAYPIVSQAVRNDRLNPEDLMNYYVTTATGQSIPLGSFVTMELKTSPSSLPRMSQL
                     NSATIGGVVSGSIGDAVNWAKAELDRTLPSGYQYDFRGESRQFVQEGNAMAMTFALAV
                     IIIYLVLAIQFESWRDPLVILVSVPLAVSGALLVMNIPTAMKGLFMGIAAGTGSKDMM
                     AFANGIWTAGYTLNIYSQVGLITLVGLITKHGILMCEVAKEEQLLHGKSRYEAIFHAA
                     TIRLRPIMMTTAAMIAGLIPLLFAVGAGAIARFSMGMVIVAGLAIGTLFTLFILPVIY
                     TFLGQEHKPLREFDEEKYFPTTKEA"
     gene            complement(5718..6923)
                     /locus_tag="PXD66_000572"
     CDS             complement(5718..6923)
                     /locus_tag="PXD66_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDL6318902.1"
                     /translation="MTTETQKLGKGRKFLIVLTLLIVLIVFAAIVGLQKIGAMKKAEA
                     AANMPEAVSEVTAMQVTTQEWTPTISAVGYIRPNQGAMLSAETSGVVSRVLVTSGQRV
                     KKGDLLVEFDSSVEIANLRASEAQLPNAKANLERYRNLIASNSASKAEFDNAQSTYNQ
                     LLANIEALRATIKRRQIYAPFSGLAGIVNVNVGQYITMGTEIVRVEDQSSMKVRFTLP
                     QTNLEQISIGQKVTAVIDALPAQTFPAKIVAIDPAVDRLTGLINVEAVIEEGQEKLLS
                     GMFARLNVALPTETGQIVVPQISIAYTMYGETVYVLQPLSDEDKEMVNKMAVQNPSLD
                     VNKMYRAKQMEVKTADRKGNYSQLSKGVKAGDLIVTGGLQRLSNNALVKVSDTEAVGI
                     TTPAKNSKL"
     gene            complement(6954..7526)
                     /locus_tag="PXD66_000573"
     CDS             complement(6954..7526)
                     /locus_tag="PXD66_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL6318903.1"
                     /translation="MSRRSEPKEEMVNRILSATESLIVQEGLQNLSMRSIAKEAGIAS
                     GTLYLYFKTKDDLLHSLAAQLLECYNGYMHFEFKPEMALFEQYKALVNKKWQFLIERL
                     DLVKQFQAVIGIDELIRETIADETSFWNKLILEGKKQRIIADLSNELLYGLSIGTLVD
                     IQYLQNLNQENQFDGYLDEIILRTWKAITF"
     gene            7765..7986
                     /locus_tag="PXD66_000574"
     CDS             7765..7986
                     /locus_tag="PXD66_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1414 domain-containing protein"
                     /protein_id="HDL6318904.1"
                     /translation="MATKSKYHTKQFEQLLADLIATIEKHKAPVDLSLMALGNMITNI
                     LIENIQSPAQREALADAFADALKQSLKTK"
     gene            7991..9724
                     /locus_tag="PXD66_000575"
     CDS             7991..9724
                     /locus_tag="PXD66_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3413 domain-containing protein"
                     /protein_id="HDL6318905.1"
                     /translation="MFARLRSLLPTNSRQYREETSQKITWGHWFALFNIVLALLISSR
                     YAFNADWPNTLVGKLYFFVSLFGHFSFVVFAGFLLILFPLSFIVKNHRTYRGLSVILA
                     TIGQTVLLVDTEVFKQFYLHLSPLVWDLLVNPEEGELTRKWQLLFVPMPLLLLAEMLY
                     SRWCWQKLRSFSRQKWGKYVALFFVISFTATHLIYAWADMNAYRPITAQRANYPLSYP
                     MTARNFLEKHGFIDRVEFNQEIEESGRLDTFYLNYPKSPLIATKTPSTNILLINLSGL
                     SKQIINAETMPKLTEISQQSHRFMQHYSSGDSASAGALGLFYSLSGKYLDSVLADKTV
                     SPLIEIARKSDYQLGLFSHNGFADPIYHQAIFSGILLPTAHSNVGAIGQWKNWISSRS
                     LENPFFSYLDLRMPFAHTAQEQAKLLDWQFAEIWQHLTQNALLANTLVIITADISNEV
                     GAENSFDKQHIQVPMMFYWQGESKEYHFLSSHLDIMPTLLSQFFGVQNPLNDYAQGMD
                     LSTQNQRLWVSSSNHKWNVAIMPNGDQYQIDRKGNFKHFNSNGEHEKDARPPLALFLQ
                     MIQQSNQFIEK"
     gene            complement(9791..10492)
                     /locus_tag="PXD66_000576"
     CDS             complement(9791..10492)
                     /locus_tag="PXD66_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL6318906.1"
                     /translation="MIGQALGWRATFGIIGIIALIVLMMIWRLLPYLPSKNAGSIKSL
                     PLLFKRPMLIGIYLLVALIVSGHFTAYSYIEPFMIKISSVSVENTTFILLVFGLAGVV
                     ASFLFGRLYGKAPNKFILSGIAIIMCSQLCLLFLSDSTVAMFMVAFVWGIGITGMSIA
                     LQMKVLELAPDATDVATAIFSGTYNLGIGGGALLGSIAIQQLGLANIGFAGGSIAFVA
                     MVWFVFVSLKYRQLR"
     gene            complement(10489..10869)
                     /locus_tag="PXD66_000577"
     CDS             complement(10489..10869)
                     /locus_tag="PXD66_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL6318907.1"
                     /translation="METLAIFLFSEIWKFDFVFTFIFKILKINVFFSDLINMKLARKA
                     QRRLSFLRVFSLAFAAFIVNTTEFIPIALLSDIASSFQMEPSSAGIMMTVYAWVVFLL
                     SLPLMLLTANMERKRLLLMLFMGE"
     gene            complement(10911..15371)
                     /gene="mukB"
                     /locus_tag="PXD66_000578"
     CDS             complement(10911..15371)
                     /gene="mukB"
                     /locus_tag="PXD66_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HDL6318908.1"
                     /translation="MIDTTELQNNPQIAPLARPNDVQRGKFLSLTLINWNGFFARTFD
                     LDDLVTTLSGGNGAGKSTTMAGFVTALIPDLTLLNFRNTTEAGSTAGSRDKGLYGKLK
                     AGVCYAVLESFNSRGQRIITGVRLQQIVGRDKKVDIRSFSLQNIPQEQSIISLLTEQV
                     GNKARVLALNELKDQFENSEVQFKQYHSITDYHSFMFDLGVIPKRLRSAADRSKFYKL
                     IEASLYGGISSVITKSLRDYLLPENTGVRQAFQDMESALRENRMTLEAIKVTQSDRDM
                     FKRLITESTNYVSADYMRNANERRGNVQQALEQRKEWYAAKSKILLEQQRFVEFSRES
                     ADIAEAELALEADYNSANDHLNLVMNALRHQEKIERYQDEVEELNIKLEEQQEALEEI
                     AEIAENAQARADEADDHVEELRSQMADYQQALDAQQTRALQYQQAVNALEKAKQLTGL
                     VNLDLNNIEDYHAEFVAQAEDLTDQVFELEQRLSVSDMAKTQFEKAFELVCKISGEID
                     RLQAWEEARALLSAFPEQKMQAQQAVSLRQKLNDLEQRLQQQQNAQRLVAEFNQKSQQ
                     QLNSAEELDAYFEEQQARLEDLEAELSDFVEQRSTQRQQREQLNQQYQQLAKNAPAWH
                     TAQSALTRLEEQCGEKFEASQSVMQFMQNMLSKEREATLARDELARKEQMLDEQINRL
                     SQPDGSEDARLNQLAERFGGVLLSELYDDVSIEDAPYFSALYGEARHAIVVRDLEAVK
                     TQLEKLDDCPSDLYLIEGDPSAFDDAVFSAEELGEGVVVKVSDRQWRYSKFPEVPLFG
                     RAAREKHLATLKAERDETAEKHAERAFDVQKCQRLHQHLSQFIGTHLNLAFQENPEML
                     MQEIAAERAEIERELAQSAGNEQQLRHQLESVKAQLQMLNKILPLANLLADETLEARA
                     EECREQLELAEEDEMFIRQFGATLSQLEPIAAALKSDPALFEQLEADYRRSVAEQKVL
                     QQKVFSLADVMNRRLHFSYQETVGTEGSALTEQLRQRLENAQREREQARDQLRQAQAQ
                     LSQYNQVLTGLRSSYEAKNQMLQELIREIDDLGVRGDSGAEERARFRRDELQQRLSQQ
                     RSRKGYLDKQIAVIEAEMDNLNRTLRKAERDYHTQRELVVQAKASWCIVQRLSRNSDV
                     EKRLNRRELAYQSAEELRSISDKALGALRTAVADNEYLRDSLRASEDSRKPENKVAFF
                     IAVYQHLRERIRQDIIKTDDPIDAIEQMEIELSRLTNELTGREKKLAISAESVANILR
                     KTIQREQQRILQLNQGLQNIAFGQVKGVRLVVNIRDTHAILLNALSNNREEHKDLFDS
                     QKLSFSEALAMLYKRVNPHIEMGQRTPQTIGEELLDYRNYLDLEVETFRGADGWMRAE
                     SSALSTGEAIGTGMSILLMVVQSWEEEARRMRAKDILPARLLFLDEAARLDATSINTL
                     FELCERLDMQLLIAAPENISPERGTTYKLVRKITNNQEYVHVVGLKGFAK"
     gene            complement(15547..16263)
                     /gene="mukE"
                     /locus_tag="PXD66_000579"
     CDS             complement(15547..16263)
                     /gene="mukE"
                     /locus_tag="PXD66_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HDL6318909.1"
                     /translation="MTENIQDFISPKLALAIANPIFPELDSQLRAGRHINTEQLDQHV
                     FLMDFQAELESFYRRYHVELIRAPEGFFYLRPKASTLIARSAMSEMEMLVGKVLCYLY
                     LSPERLAQQGIFSQDDVYEELLSLADESKLLKAVNPRSTGSDLDRAKLAEKAGGALRR
                     LARIGIITRVGEQNSKKFVISESVFRFGADVRTGDDPREAQLRLIRDGEATTPALLNE
                     QAVEIDENIADEAPFDGENE"
     gene            complement(16312..17655)
                     /gene="mukF"
                     /locus_tag="PXD66_000580"
     CDS             complement(16312..17655)
                     /gene="mukF"
                     /locus_tag="PXD66_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HDL6318910.1"
                     /translation="MQNELAQTIPELISWTKDREFSLSLSSDRLAFLLAISIYNQEQT
                     DGELLESDLIDLFRYVSNSFEQSDATFTQRANNAINDLVRQRLLNRFSSEFTEGLAIY
                     RVTPLGVGISNYYVRQREFSTLRLSIQLSIVADEIQRASVAAEEGIEQAQDERFWRNN
                     VFAPLKYSVAEIFDSIDLSQRIMDENQHQIRERIAELLSQNWHEAIMSCEQLLDETSG
                     NLRELQDTLNAAGDKLQAQLLRIQSCLMARNDLDFIDQLIVNLQNKLDRIISWGQQAI
                     DLWIGYDRHVHKFIRTAIDMDKNRVFGQRLRQSIQDYYNSPWLLYTAKADSLLDLRDD
                     EAMLNEAESVGELPSELEYESLSDVQEQIVSAMQAHLAPFRAEGKPIDLGVILREQLA
                     RFPESRHFDVARIIVDQAVKLGMASQDSQAVYPQWQPINEQGAEVQANVIDQYNK"
BASE COUNT         5023 a         4105 c         3420 g         5217 t
ORIGIN      
        1 caagtacttg aagtagaaac attgacaaaa tttctataaa aatcgaccgt ttattaactt
       61 ggagtattac atttttgaag aatcttcaaa atctctttca tatctttgcc catataatcc
      121 gctaaacttt ggtcaatcag tattcgtttt tcttcgtaac caatcaccgt taagcctgcg
      181 gttttggcgg cgagcattcc gtagtaggag tcttcaatcg ccactgcatt ttgtggctca
      241 acgcctaatt tttcgcaggt gtagcggtaa atggttgggt ctggcttgct gtgttcaaac
      301 tgttcaccgc tgacgattaa atcaaattcg tctttaatgc cgcaaaccgt gagaatattt
      361 tcaatatgcg aaagggcgga agaagaggca accgcaagct taatttgatt ctgcttggca
      421 aattgaataa tttgctgaat atcggctctg aaaatggttt taaaatccac tttgctaaac
      481 agatcttgga aaaaggcgta gtaacgtgca cgaatttcct caatcggcaa actggattcg
      541 cttagttttt tgataatcac cggaatttct tttaaacatt tgcctaccac ttccgattgt
      601 tgttctaaag taatcgggcg gctgtgttct ttgatgtctt caataaactg tttttgtaag
      661 gaaaattcca gatattcggt atccacaatc acgccgtcca tatcaaaaat gatagcttgg
      721 agcatatagc ctcttttgta aaattttaag aaaatctaac cacttattcc ctctctctgt
      781 tttaaattac tgaacgtaat ttaaaacggt ctctctccca caaggggaga gagtgagaaa
      841 ggctattaac ccattcggta gttcggtgct tctttggtaa ttgtcacatc gtggacgtgg
      901 ctttctttaa taccagcacc actaatgcgg acaaattctg ctttggtgcg gagttcttca
      961 atagtggcac agccggttaa tcccatacag gagcgtaagc cgcccatttg ttggtggata
     1021 atttctttca agtagccttt gtaagcgata cgcccttcaa tcccttccgg tacgagcttg
     1081 tcggcggcat tatcagattg gaaatagcga tctgacgagc ctttactcat tgcacctaat
     1141 gatcccattc cacggtagga tttgaatgct ctgccttgat aaagctcaat ttcacccggg
     1201 gcttcttctg tgccggcaaa catcgaaccg accattacgc aacttgcacc ggcggcaata
     1261 gcttttgaaa tatcgcctga ataacgaatt ccaccatcag caatcacagg aatgcctcgt
     1321 tctttaagcg cagctgccgc ttctgcgatt gccgtgattt gtggcacgcc aacgcctgtt
     1381 acaattctgg tggtacaaat tgaacccggg ccgattccta ctttcacagc actggctcct
     1441 gcatcagcta acgcaatcgc tccttctgca gtggcaatat taccggcaac aatcggtaaa
     1501 tcagggtatt ttgcacgggt ttcacgcaca cgttgtaata caccttcaga atgcccgtgc
     1561 gaagagtcga ttaatagcac atcgacaccc gcttttacta aagcatcaat tcgttcttca
     1621 ttgcccggac cggctcccac tgccgccccc acacgcaaac gcccaaactc atctttacag
     1681 gcattcggtt tgctttccgc tttttggtag tcttttaggg taatcattcc ttttaactga
     1741 aattcattat tcactaccag cactttctcc actcggtgtt cgtgcattaa gtggaaaatt
     1801 tcttcacggc ttgcgttttc ttttaccgtc actaaacggt cttttggtgt cataaattca
     1861 cgcactgttt tgcttaaatc cgtgacaaat cgggtatcac gtccggtaat aattcccact
     1921 aaattttggt tttcatcaat caccggatag cctgcaaaac cgttcttttt caccaattca
     1981 gccaattccg ctaatgtcat atcaggagaa atcgtcactg gctcagaaac aataccactt
     2041 tcaaattttt tcactttacg cacacggtct gcctggcgtt caatcgacat atttttatgg
     2101 ataaaaccaa tgccgccttc ttgagcaagg gagatcgcaa gcttagtttc tgtaacggta
     2161 tccattgcag cagaaagcat cggaatgttt aaacgaatgg ttttagttaa ttgagtagaa
     2221 agatcagcag tattaggaag cacagtagaa tgtgccggga cgagaagaac atcatcaaaa
     2281 gtgagggctt cttgtttaat tcgtagcatt gcaattattc cttaaaaaat gattaaattg
     2341 aaaatctgta ctggaaaatt gcgagcggat tatacagagg aactaggttt ctgtaaatag
     2401 ggaaacgttt acgctatctg aaaatatact ttcaagcggt taaattttgt gattttttag
     2461 caaacaataa ataaaaaacg accgcttgta agccaacaaa aaagagcgta tcgctacgct
     2521 cttttgctat ttaaaactat gcttcttttg tcgttgggaa atatttttcc tcgtcaaatt
     2581 cacgtaatgg cttgtgttct tgccctaaga aagtgtagat aaccggcaag ataaacagag
     2641 taaacaatgt cccgattgcc aagcctgcta cgattaccat acccatactg aagcgggcga
     2701 ttgcaccggc acccaccgca aacagaagcg gaattaagcc tgcaatcatt gctgccgttg
     2761 tcatcataat tgggcgtaaa cgaatcgtgg ctgcgtggaa aatagcttca tagcgtgatt
     2821 tgccgtgcag taactgctct tctttcgcca cttcacacat caagataccg tgtttagtaa
     2881 ttaagccgac aagggtaatt agacctactt gtgaatagat atttaaggta taacctgccg
     2941 tccaaatacc gttggcgaat gccatcatat ctttcgagcc agtgcctgct gcaatcccca
     3001 taaacaagcc tttcattgcg gttggaatgt tcattaccaa caatgcacca ctcacagcta
     3061 acggcacaga aaccaaaatt actaatgggt cacgccaaga ttcaaattga attgcaagta
     3121 caagatagat aatgataact gctagagcga aagtcattgc catcgcatta ccttcttgaa
     3181 cgaactggcg agattcacca cggaagtcat attgataacc tgatggtaaa gtgcgatcta
     3241 attctgcttt cgcccaattt accgcatcgc cgattgagcc agatactacc ccaccaatgg
     3301 tggccgagtt taattggctc atacgaggta aagaagatgg tgatgttttt aattccatcg
     3361 tcacaaatga acctaacggg atagattgcc ctgttgcagt cgtgacatag taattcatta
     3421 aatcttcagg atttaaacga tcattacgca ccgcttgcga cacaatcggg taagcacgag
     3481 agtcaatatc gacacgagta ataatcgcac cggataagaa tgcccctaat gtgccactca
     3541 cctgctgcat tgtcacgcca taagcaccca ttttttcacg gtcgaatttc atattcatcg
     3601 tgaccgtatc aaatttcaaa tctaaattcg caaagaagaa ctgaccggaa gtttgggctt
     3661 ttttcaagaa ttcagaggca acgattgcca atttttcata gctatcagcg gtagtaatca
     3721 ctaatgagaa tggtagacca ttttcaccgg ttgaaatttc agggaatgca attgcattca
     3781 cgtccatacc caccactttt ttaccgattg cagccacatc attagaaatc gcagcacgtt
     3841 cacggctacg atcattccaa tcttttaatg caataatcga taatgcacta tttgcaccat
     3901 taaagcctga aactgtcatt aaggaagata cttcaggtaa tttagttact tcctgattaa
     3961 attgggctaa cgctgcttcg gtataatcta aattggtgtt tgaaggtcca ttagaaatac
     4021 caaccacaaa gccacggtct tctgtcggtg caacttcact ggaaagtgag ccaaataaca
     4081 tcggtaaact ggcgaagatc agcaccgcaa agaataagac gaaaccacga acagccataa
     4141 taccgcttag catattggta tagcagctgg tcattttatc tagtgatcta tgcaccacgc
     4201 gggcaaatgc tgactcttcc ccttctttat gttcttttaa tattcggcta gacatcatcg
     4261 gcgaaagggt taatgccgca atccccgaaa taaataccgc accggcaagc gttaaagcga
     4321 actctttaaa tagtgtccct gttaccccgg tcattaatgc cattggtgaa tatactgcac
     4381 ccagagtaat agtcattgaa ataaccggta aagcaatttc acgtgtaccg ataatcgctg
     4441 catcaaatgg cgttttacct tctttaacgt gacgttctac gttttcaagt acgacaatcg
     4501 catcgtccac taccaagccg atcgctaata ccaacgccag caaagtaagc aagttgatcg
     4561 agaaaccaaa catttgcagg aagaataata ccccgattaa ggaaatcgga atggtaatca
     4621 ccggtacaat cattgctcgt aatgaaccta agaacaggat aattactacc aatacaatga
     4681 cggttgcctc accaatagtt ttaattactt ctttgataga actatcaatc gcaatggttt
     4741 tatcgtaaac aatatctcct tgaatggtgc ttggtagatt ttttaatacc tgctcaaaca
     4801 tcggataaat atctttcgct acggttaaag agtttgccgt tgcggctacc gaaacagcaa
     4861 gcacaactgc atctttaccg ccaaccgttg ctcttgctgc atcttggtat ttatccagct
     4921 caacagtcgc aatatctttt aatttgatgg tttttccatc tttggtggtt gaaaccgtaa
     4981 cctcttctaa ctctgtcaca gaaggcgtgg tagaaagtac tttgtttcta taaaccgtat
     5041 agtaaccgtt agaattaccc gcagcggttt gcaagttatt ggcacttaat gccgataata
     5101 cagccgcccc tgataggtta ttgctcgcca ttttatccgg atcaagccag atgcgtaagc
     5161 caaaaacaga gccatacaca tcaacgctag aaacaccatt taccgtaaag aactgtggtt
     5221 ttactacacg gttaatataa tcggtaactt gtgatgtatt aagttcatca gagacaaatc
     5281 ggatatacat taacgcatca tttgaaccgg ttgagactct aattgacgga tcatcaatac
     5341 cgctcggtaa atttgagcgt accgcattca cttttgccga aatatcggaa agtgcggcat
     5401 tcggatcggt atttaacttc atttttgccg ttaccgtagt ggtacttgga gagcttgatg
     5461 aagtcatata gtcaatatta tccgcttgag caacagcctc ttctaactgt gaggtaacca
     5521 gtgtctgcat taaacgtgca tctgcacctg aatagttaac actcacggtt acaatcgaga
     5581 ccgtcatctc cggatattca cgcacctgca atttttcgat tgcttgcaag cctaggatcg
     5641 taatcaatag gctgatacaa actgccaaaa caggacgttt aataaagatg tcagtaaatt
     5701 tcacttaact cactccacta cagtttactg tttttcgccg gtgtggtaat gcctacggct
     5761 tctgtatcag aaactttaac caacgcatta ttacttaaac gttgtaaacc gcctgttaca
     5821 atcagatcac ctgctttcac gcctttggat aattgtgaat agtttccttt gcggtcagcc
     5881 gttttcactt ccatctgttt tgcacggtac attttgttta catctaaact tgggttttgt
     5941 accgccattt tgttcaccat ttctttatct tcatcagata atggttgcag cacatatacc
     6001 gtttcaccat acatagtgta agcgatagaa atttgtggta cgacgatttg tccggtttct
     6061 gttggtaaag caacatttaa gcgggcaaac atacctgaaa gcaatttttc ctgcccttct
     6121 tcaatcaccg cttccacatt aattaagccg gttaaacgat ccacagccgg atcaatagcc
     6181 acaattttag ctgggaaggt ttgtgccggt aacgcatcaa ttacggctgt cactttttgc
     6241 ccaatcgaga tttgctctaa attagtttgc ggcaaggtaa aacgtacttt cattgaagat
     6301 tgatcttcca cacgcacaat ttccgtaccc attgtaatgt attgcccaac attcacattc
     6361 acgatacccg ctaaaccact aaacggagca taaatttgac ggcgtttaat cgtagcacgc
     6421 aatgcctcga tattggctaa taattggttg taggtagatt gagcattatc aaactcggct
     6481 ttagaagcac tgtttgaggc aatcagatta cggtaacgct ctaaatttgc tttggcattc
     6541 ggtaattgcg cttcagaggc tctcaagtta gcaatttcaa cactgctgtc aaattctact
     6601 aacagatcac cttttttcac acgctgtcct gaagtgacca aaacacggct aaccacgcct
     6661 gaagtttcag cacttaacat tgccccttga tttgggcgaa tataaccgac cgcagaaatg
     6721 gttggtgtcc attcttgggt cgtcacttgc atagcagtaa cttcgctcac cgcctccggc
     6781 atatttgccg ctgcttcggc ttttttcatt gccccgattt tttgtaaccc tacgattgca
     6841 gcaaacacaa tcaatacgat taacaatgtg agtacaatta aaaactttct gcctttaccc
     6901 agcttctgtg tttccgttgt catacattct ccataaaact aataaaaaga aacttaaaaa
     6961 gtgattgctt tccaagtgcg taaaataatc tcgtctaaat atccatcaaa ctgattttct
     7021 tgattcagat tttgcagata ttggatatcc accaatgtgc cgatgcttaa tccataaagc
     7081 aactcattag atagatcagc aataatcctt tgctttttcc cttctaaaat taatttattc
     7141 caaaaggaag tttcatcagc tatggtttcc cgaatcaatt catcaatccc tatcaccgct
     7201 tgaaactgtt ttaccaaatc taaacgctcg attaaaaact gccatttttt attgaccaac
     7261 gctttatatt gctcaaataa cgccatttca ggcttaaact caaaatgcat ataaccgtta
     7321 taacattcga gtaattgggc agccaatgag tgcaataaat catctttggt tttaaagtaa
     7381 agatataatg taccggaagc gatccctgct tcttttgcaa tactacgcat tgaaaggttt
     7441 tgcaaacctt cttgcacaat cagcgactcg gttgccgata aaatacggtt taccatctct
     7501 tcttttggct cgcttcgcct agacatttta tctccttacc tataatgaac agccgttcat
     7561 tatagctttt tttatgaaaa aagaaagcag aaaacaccta tcaaattaaa ataaaatagt
     7621 tgcaaaattc ctcatttatt gaacaagcgg tcatttttgg ctaaaacttt gcaaaaatca
     7681 ctcaaacaat aacgcttaac gctactttat tattattttt gtgctactat ttgtacctaa
     7741 ttataggatc atctttattt caatatggca acgaaatcta aataccacac caaacaattt
     7801 gagcagctat tggctgacct catcgccacc atagaaaagc ataaagcacc ggtggattta
     7861 tcgttaatgg cattaggcaa tatgataact aacattttaa ttgaaaatat tcaaagcccg
     7921 gcacaaagag aagctctcgc tgatgccttt gccgatgcat taaaacaatc actaaaaacc
     7981 aaataagatg atgtttgcac gcctacgcag tttattgccg accaattctc gccaatatcg
     8041 agaagaaacc tcacagaaaa tcacgtgggg gcattggttt gcactattta atattgtgtt
     8101 ggcactactc atttcctctc gttacgcatt taatgcagac tggccgaaca cgcttgtagg
     8161 caaactttat ttctttgtca gcctgttcgg gcatttcagc tttgtggtct ttgcaggatt
     8221 tctattaatt ctgtttccgc tcagttttat tgttaagaat catcgcacct atcgtgggct
     8281 ttccgtgatt ttagccacca tcgggcaaac cgtgttattg gtggatactg aagtcttcaa
     8341 gcaattttat ctgcatttat caccgcttgt atgggatctg ttagttaatc cggaagaagg
     8401 ggaactgacc cgtaaatggc aactgctgtt tgtaccaatg ccactccttt tgctggcaga
     8461 aatgctctac tctcgctggt gctggcagaa actgcgtagt tttagccgcc aaaaatgggg
     8521 caaatatgtc gctctctttt tcgtgatcag ctttaccgct actcatttaa tctatgcgtg
     8581 ggcagatatg aatgcctacc gcccgattac cgctcaacga gcaaactacc cactttctta
     8641 cccgatgacg gctcgtaatt tcttagaaaa acacggtttt attgaccgag tagaatttaa
     8701 tcaagaaatt gaagaaagtg gcagactaga tactttctat ctgaattatc caaaatctcc
     8761 cttgattgcg accaaaacgc cctcaactaa tattttactg attaatttat caggtttatc
     8821 taaacagata ataaatgccg aaacaatgcc taagctgaca gaaatcagcc aacaaagcca
     8881 ccgctttatg cagcattaca gtagtggtga ttcagcctct gccggtgcat taggtctatt
     8941 ttacagttta agcggtaaat atttggattc cgttttagcc gataaaaccg tgtcaccgct
     9001 tatcgaaatc gctagaaaat cggactatca attagggctg ttttcacata atggttttgc
     9061 cgacccgatt tatcatcagg cgatcttctc tggtatttta ttgcctacgg cgcacagtaa
     9121 tgtaggggcg attgggcaat ggaaaaactg gatcagctct cgctcattgg aaaatccgtt
     9181 ctttagctat ttagacttaa gaatgccctt cgcccacacc gctcaagagc aggcaaaatt
     9241 attagattgg caattcgctg aaatctggca acatttgacc caaaatgcac tattggcgaa
     9301 tacgctggta atcattaccg ccgatattag taatgaagtt ggagcagaaa atagcttcga
     9361 taaacaacac attcaagtgc cgatgatgtt ctactggcaa ggcgaaagca aagagtacca
     9421 tttcctttca agccatttag atattatgcc aaccttactg agccaattct tcggcgtgca
     9481 aaatccgctg aatgattatg cacaaggtat ggatttaagc acccagaacc aacgtttatg
     9541 ggtatccagt tctaatcaca aatggaatgt ggcgattatg ccgaatggcg atcaatatca
     9601 aattgatcgt aaaggcaact ttaagcactt taacagcaat ggcgaacacg agaaagatgc
     9661 tcgaccacca ttagcgttgt tcttacaaat gattcaacaa agcaatcaat ttattgaaaa
     9721 gtaatgttca acaagcggtc atttttgcag gaaaatttgc aaaatttcag caaaatatga
     9781 ccgcttgtca ctatctgagc tgtctatatt tcaagctaac aaatacaaac caaaccattg
     9841 ctacaaaagc aatactccca ccggcaaatc cgatattggc taatcccagt tgctgaatag
     9901 cgatgctccc cagtaatgct ccaccgccaa tgcctaaatt ataagtgccg gaaaaaattg
     9961 cagttgcgac atcagtcgca tcaggggcaa gttcaagcac cttcatttgc aacgcaatgc
    10021 tcattccggt aatgccaatt ccccagacaa aagcgaccat aaacattgcc actgttgagt
    10081 cactcaaaaa cagtaaacaa agctggctgc acataataat ggcgatcccg cttaagataa
    10141 atttattcgg ggctttgcca tataaccgac caaataaaaa acttgccacc acacctgcca
    10201 agccaaaaac cagcaaaata aaggtggtgt tttccacact caccgaactg attttaatca
    10261 taaacggctc aatatagctg taagcggtaa aatgtcctga aacgatcaat gccaccagca
    10321 aataaatgcc gattaacatc gggcgtttaa ataacaaagg caaacttttt atcgagcctg
    10381 catttttgct tggtaaataa ggcagcaacc gccaaatcat cattagcaca atcaaagcga
    10441 taatcccaat aatgccaaag gttgcccgcc agcctaaggc ttgcccgatc attcgcccat
    10501 aaataacatc agcaacagcc gttttctttc catattcgcc gtgagtaaca tcaacggtaa
    10561 cgataataaa aataccaccc acgcataaac cgtcatcata atgcctgccg atgaaggctc
    10621 catttggaaa ctgcttgcaa tgtctgaaag caatgcaata ggaataaatt cggtggtgtt
    10681 tactataaaa gccgcaaagg caagactaaa tactctaagg aacgataatc tccgttgagc
    10741 ttttcgagca agtttcatat taattaaatc cgagaaaaac acatttattt tcaaaatttt
    10801 aaaaataaat gtaaaaacaa aatcaaattt ccaaatttct gaaaataaaa agattgccaa
    10861 tgtttccatc aacaatcttt ttatacatat ttagattaaa gtgctaagtt ttatttagca
    10921 aagcctttta aaccaacaac gtgaacatac tcttggttat tggtgatttt acgcaccaat
    10981 ttataagttg tgcctcgttc agggctaata ttttcagggg ctgcaattaa taactgcata
    11041 tccaaacgtt cgcagagttc aaatagcgta ttaattgagg tagcatctaa tcgagccgct
    11101 tcatctaaga acaataagcg agccggtaga atatctttgg cacgcatacg gcgagcttct
    11161 tcttcccagc tttgaactac cattaagaga atcgacatac ctgtaccgat cgcctcaccg
    11221 gtcgataatg cactactttc tgcacgcatc cagccgtctg caccacggaa ggtttccact
    11281 tctaaatcta agtagttacg gtaatccaat aactcttcac caatggtctg tggtgtacgt
    11341 tgccccatct caatatgagg gttcacccgt ttatacaaca tcgctaaggc ttcggagaag
    11401 ctcagttttt ggctgtcgaa taaatctttg tgttcctcac gattattgga taacgcattg
    11461 agtaaaatag cgtgcgtatc tcggatattc accaccaaac gcaccccttt tacctgaccg
    11521 aatgcaatat tttgtaaacc ttggttcagt tgcaaaatgc gttgttgttc acgctgaatg
    11581 gttttacgta aaatatttgc cacactttcc gcactgatcg ccagtttttt ctcacgaccg
    11641 gttaattcat tggttaagcg tgaaagctcg atttccatct gctcaatcgc atcaatcgga
    11701 tcatcggttt tgataatgtc ttggcgaatc cgctcacgca aatgctgata aaccgcaata
    11761 aagaacgcca ctttgttttc cggcttgcga ctatcttcag aagcccgcaa gctatcacgc
    11821 aggtattcat tatccgccac ggcggtgcgt aatgccccga gagctttatc cgaaatcgaa
    11881 cgtaattctt cagccgattg ataagccaat tcacggcgat ttaaacgttt ttccacatcg
    11941 ctgttacgag ataaacgttg cacaatacac cagctggctt ttgcctgcac caccaattca
    12001 cgttgtgtat gataatcacg ttcggcttta cgtaaggtgc ggttaaggtt atccatttcc
    12061 gcctcgatca ccgcaatttg tttatctaaa taacctttgc gggaacgttg ctggcttaaa
    12121 cgttgttgca attcatcacg gcggaatctg gctcgctctt ccgctccgct atcaccacgc
    12181 acgcccaaat cgtcaatttc acgaattaac tcttgtagca tttggttttt cgcctcataa
    12241 gaactgcgta agccggtcaa tacttggtta tattgtgaaa gttgagcttg agcttgacgc
    12301 agctgatcac gagcctgttc acgctcacgt tgtgcatttt ccaaacgttg gcgtagctgt
    12361 tcggttaatg cagagccttc tgtacccacc gtttcttgat agctaaaatg gaggcggcga
    12421 ttcattacat cagccaagct gaacactttt tgctgtaaca ctttttgctc ggcaactgaa
    12481 cggcggtaat cggcttccaa ctgttcaaat aaagccggat cactttttaa tgcggctgca
    12541 atcggctcaa gttggcttaa tgttgctcca aattggcgga taaacatttc atcttcttct
    12601 gccaattcta attgctcacg acattcctcc gctcgtgctt ccagcgtttc atcggctaat
    12661 aaattcgcaa gcggtagaat tttattcaac atttgcaact gggcttttac gctttctaat
    12721 tggtgacgta attgctgttc gttaccggct gattgtgcca attcacgttc aatttctgcc
    12781 cgttctgccg caatctcttg cattaacatt tcagggtttt cttggaacgc taaatttaag
    12841 tgcgtgccga taaattggct taagtgttga tgtaaacgct ggcatttctg cacatcaaag
    12901 gcacgttcag cgtgtttttc tgcggtttca tcacgctctg cttttaaagt cgctaaatgt
    12961 ttttcacgag cagcacgtcc gaataacggc acttcaggga atttagaata acgccattgg
    13021 cgatcggaga ctttcaccac cacgccttcg cctaattcct cggcactaaa taccgcatcg
    13081 tcaaaggcgg acggatcacc ttcaattaaa taaagatcac ttgggcaatc gtctaatttc
    13141 tctagctgtg ttttaaccgc ctctaaatca cgcactacaa tagcgtggcg agcctcgccg
    13201 tataaggcgg agaaataagg cgcatcttca attgatacat catcatataa ctcagagagt
    13261 aacactccac caaaacgttc tgccaactgg tttaagcggg cgtcttccga gccgtccggc
    13321 tggcttaagc gattaatttg ctcatcaagc atttgttctt tacgagccaa ttcatcacga
    13381 gcaagggtgg cttcacgctc tttgctcagc atattttgca taaactgcat cactgattgg
    13441 ctggcttcaa acttctcgcc acattgctct tctaaacgag tcagagccga ttgtgccgta
    13501 tgccaagccg gtgcattctt cgctaattgc tgatattgtt gattaagctg ttcacgttgt
    13561 tggcgttggg ttgaacgttg ttctacaaag tccgaaagct ctgcctctaa atcttctagg
    13621 cgagcttgct gctcttcaaa ataggcgtct aattcttccg ctgaattgag ttgttgttgc
    13681 gatttttggt taaattccgc cactaaacgc tgggcatttt gctgttgttg caaacgctgt
    13741 tctaaatcgt tgagtttttg acgtaaactc accgcttgct gagcttgcat tttttgctca
    13801 gggaaggcag aaagtaacgc acgagcctct tcccacgctt gcaagcggtc aatttcaccg
    13861 gaaattttgc aaaccaattc aaacgctttt tcaaattggg ttttcgccat atccgacacg
    13921 cttaaacgtt gttccagctc aaatacttga tcggtaagat cttcggcttg agccacaaat
    13981 tctgcgtggt aatcttcaat attatttaaa tctaaattca ctaagccggt tagctgtttg
    14041 gctttttcta acgcattcac cgcttgttgg tattgcaatg cacgagtttg ctgtgcatct
    14101 aaggcttgtt ggtaatccgc catttgagaa cgcaattctt caacgtgatc atctgcctca
    14161 tccgcacgag cctgagcatt ttcggcaatt tccgcaattt cttccaatgc ctcttgctgt
    14221 tcttccagct taatattcag ctcttcaact tcgtcttggt agcgttcgat tttctcttgg
    14281 tggcgtaacg cattcatcac taaatttaga tgatcgttcg cactattgta atccgcctct
    14341 aacgccagct ctgcttcggc aatatccgca ctttcacggc taaattccac aaagcgttgc
    14401 tgttcgagca aaattttgga ttttgccgca taccactctt tgcgttgctc taaggcttgt
    14461 tgtacattgc cacggcgttc attcgcatta cgcatataat ccgccgacac ataattggtc
    14521 gattcggtaa ttaagcgttt gaacatatcc cgatccgatt gcgtgacctt aatcgcctct
    14581 aaggtcatac ggttttcacg cagagcggat tccatatctt ggaacgcttg gcgaacgccc
    14641 gtattttccg gcaacaagta atcacgcaag gatttggtaa tcacacttga aataccacca
    14701 taaagcgagg cttcgattaa tttgtagaat ttgctacggt cggctgccga acgaaggcgt
    14761 tttgggatta cgcccaaatc gaacataaaa ctgtggtaat cagtaatcga atggtactgt
    14821 ttgaattgga cttcgctgtt ctcaaattga tctttgagtt catttaacgc cagcactcga
    14881 gccttattcc ccacttgttc ggtcagtagg ctgataatgc tttgctcttg aggaatattc
    14941 tggagcgaga acgaacgaat atcgactttt ttatcccgtc cgacaatttg ttgcaaacgc
    15001 acgccggtaa taatccgttg cccacgggaa ttgaatgact ctaacaccgc atagcaaaca
    15061 cccgctttta acttaccgta taagccttta tcacgagaac cggcggttga gcctgcttca
    15121 gtcgtattac ggaaattaag caaggttaaa tctgggatca aggcggtcac aaaccctgcc
    15181 attgtggtgg acttacctgc cccgttaccg cctgaaagcg tggtcactaa gtcatctaaa
    15241 tcaaaggttc gagcaaaaaa accgttccag ttaatcagcg ttaaagagag aaatttaccg
    15301 cgttgtacat cattcgggcg tgcaagcggt gcaatttgag gattattttg caattctgtg
    15361 gtatcaatca ttatgttttc tcactaattg tcctagcacg ctaaagcgta ctaggttagg
    15421 tataggtgag tagctccgct actctaatcg caacctcagc cttggagggc tgctttatgc
    15481 ttaacctagt tcggctttac cgtgtctagg gcagactcat taaatgctga atctattcga
    15541 atgaaatcac tcattttctc cgtcaaaggg ggcttcatct gcaatatttt catctatttc
    15601 aaccgcttgt tcgtttaaca acgcaggggt tgtcgcttca ccatcacgaa ttaagcgaag
    15661 ctgtgcctct cttgggtcat caccggtacg cacatcagcc ccaaaacgga agaccgattc
    15721 tgaaatcaca aattttttgc tgttttgctc gcctactcgg gtaataatgc cgatacgggc
    15781 taatctgcgt aacgctccgc cggctttttc tgccagcttg gcacggtcta aatcggaacc
    15841 ggtcgaacgt gggtttaccg cttttaataa tttgctttca tcggcaagac tgagtaattc
    15901 ttcatacaca tcatcttggc tgaaaatccc ctgctgagcc aaacgttccg gacttaaata
    15961 gaggtagcac aacaccttac ccactaacat ttccatttct gacattgccg aacgggcaat
    16021 tagtgtcgag gctttcgggc gtaggtagaa aaagccttcc ggtgctcgaa tcaactcaac
    16081 gtgataacgg cggtaaaaac tctctagttc cgcttgaaaa tccatcaaaa agacgtgttg
    16141 gtcaagctgc tcggtattga tatggcgacc ggctcgtaat tggctgtcta gctccggaaa
    16201 aatcgggttg gcaatcgcaa gtgccagttt cggggaaatg aaatcttgga tattctctgt
    16261 cattgtcatt ctcttcaatt ttgcaaaatt ctgggtaaaa acgaccgctt gttatttatt
    16321 gtattggtcg ataacattcg cctgcacttc cgccccttgc tcgttaatcg gctgccattg
    16381 tgggtaaacg gcttggctat cttggcttgc catacctaac ttaacagctt gatcgacaat
    16441 aatacgagcc acgtcaaaat ggcgggattc cgggaatcgg gcaagctgtt cacgcaagat
    16501 aacgcctaaa tctatcggtt taccttcagc acggaacggc gctaaatgag cttgcattgc
    16561 cgaaacaatt tgctcttgca catctgaaag cgactcatac tctaactcac ttggcaactc
    16621 gcccaccgat tctgcctcat ttaacattgc ttcatcatca cgcaagtcca gtaacgaatc
    16681 ggcttttgca gtataaagca accaagggct gttgtaataa tcttgaatcg attggcgtaa
    16741 ccgttgcccg aatacacggt ttttgtccat atcaatcgct gtacgaataa atttatgcac
    16801 atggcggtca tagccgatcc aaagatcgat agcttgttgt ccccagctga taatacggtc
    16861 taacttattt tgtagattca caatcagctg atcgataaaa tccaaatcat tacgagccat
    16921 caggcaactt tgaatccgca ataattgagc ttgcagttta tcaccagcgg catttaaggt
    16981 atcttgcaac tcacgcaaat tccccgaggt ttcgtctaac aattgctcac aactcataat
    17041 tgcttcgtgc cagttttggc taagcagttc ggctattcgt tcacggattt ggtgctgatt
    17101 ttcgtccata atccgttgtg aaagatcgat gctgtcaaag atttccgcca ccgaatattt
    17161 caatggggca aacacattat ttcgccaaaa acgttcatct tgtgcctgct ctattccctc
    17221 ttccgctgct actgaagctc gctgaatttc atctgccaca atcgaaagtt gaatcgataa
    17281 acgcagggta gaaaattccc gctggcgaac gtaataattt gaaattccca cccctaacgg
    17341 cgtaacacga taaatcgcca aaccttccgt aaattcgcta ctaaaacggt ttaacaaccg
    17401 ttgtctgact aaatcattaa tcgcattatt ggcacgctgg gtaaaagtgg catcagattg
    17461 ctcaaacgaa tttgaaacat aacggaaaag atcaataaga tcactttcta acaattcacc
    17521 atctgtctgt tcttggttat aaatagagat agcaagtaga aacgctaagc ggtcggaaga
    17581 aagcgaaagt gaaaattcac gatctttcgt ccaagagatg agttctggaa tagtctgtgc
    17641 caattcgttc tgcatacggg cttaatcttt aatttctata caaaaatggt atttattgta
    17701 actgaatgta atcaaaatgg gaatggataa aacgcagaca agagaaatga acaatataag
    17761 cggtc
//
LOCUS       DANLXF010000029        17460 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.029, whole genome shotgun sequence.
ACCESSION   DANLXF010000029 DANLXF010000000
VERSION     DANLXF010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 17460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17460
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.029"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..519
                     /locus_tag="PXD66_000581"
     CDS             <1..519
                     /locus_tag="PXD66_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfJ domain-containing protein"
                     /protein_id="HDL6318911.1"
                     /translation="FQDIEDYMIHEGYLHGIPQTWKHLVSHSEQWHQHILEEQRRDQH
                     ILEEQRRENDEAHQALLSTSWSSPIDICYINEIEFQPLTTGQMLFDEGQRMRHCIFTY
                     IDICSEGRYLAFSVKIANESATLGLIKHRGNWKIEQFRYACNIAVCEPMILAAAQKLC
                     KLLNKQSALPQN"
     gene            615..1247
                     /locus_tag="PXD66_000582"
     CDS             615..1247
                     /locus_tag="PXD66_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318912.1"
                     /translation="MKTKISLIAVFMLGLSGCVHQQNTAQNSDEITPMPDSLLKPQMQ
                     PLPPQDRIVSKDIYHEQTTSNTEVVRDGRYTLISIAPEEGQKYLLEQVVSVKVPNTKN
                     RFTANVQQGLRTTLTNTGLELCFPPIGEQGNAVQTLFTRPLPKVHYQFGPMKLYEALQ
                     MIAGPAYELTLNDITRTICFKARESSFQNPHKPAVAIQRQQLKPRSLRNN"
     gene            1259..1996
                     /locus_tag="PXD66_000583"
     CDS             1259..1996
                     /locus_tag="PXD66_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03759 family integrating conjugative element
                     protein"
                     /protein_id="HDL6318913.1"
                     /translation="MKRNTINIWLAVLSSVTTNALAQTTSIESSTQTLSQQQSQHQQS
                     QLSDSLKQAKEWGLTIEEWNRYTELMKGERGMWSPNLDPLTALGIEARTAEEREKYAR
                     MLAKKYYERVSKELDFDKVYRQEFEKLYPNELPFEVEPHISQSIGRVIYFTRFDNCDS
                     CKSDLSKILSYANSQTPLDIYIVGQNISDNDIRKWAAENNIDPIKVSKKLITLNHDKG
                     YWFQYSLGKMPAAYQIKQDGEWQSLVY"
     gene            1975..2739
                     /locus_tag="PXD66_000584"
     CDS             1975..2739
                     /locus_tag="PXD66_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HDL6318914.1"
                     /translation="MAISRLLIVITLFAVGISDAKSKPFTGFGTEIDSYIRIAAQRYQ
                     ISEPMLRGLIKMEDGWYGNISPTGATGVGQFTVGTWNWLSETERGKQLGMRLITGKNK
                     GTYADPRRDKYTNTLATALLARWHIEQFHLRRIPATDENLYMAHNIGLDSLHRAMMGK
                     ATKEDILNMRRNGMKSWMTVRDFLNYQKGRFLAHKQEANFMLVSKQGHIHTGVSAFVY
                     PTSEMGKSVKWVEPDNKIIWVEPKSAMIWVEPTNTP"
     gene            2748..3254
                     /locus_tag="PXD66_000585"
     CDS             2748..3254
                     /locus_tag="PXD66_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HDL6318915.1"
                     /translation="MQHKIKMLLLSLCISHSVWGDLTVIADFGGESAVRFYEALQPEH
                     DENAPNYPNSIPASVSEADILPVVSHKLSPGIIETKQFDLTGIQAIFLIGADNTSAQW
                     LQQNREKLITLNATGLVVNVRTLAELNQLREIVPEIIMLPIPGDDLAERIGIAHYPAL
                     ITETGVSQ"
     gene            3254..5458
                     /gene="traD"
                     /locus_tag="PXD66_000586"
     CDS             3254..5458
                     /gene="traD"
                     /locus_tag="PXD66_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HDL6318916.1"
                     /translation="MSAKHTLEAWLRPPVEFFPAIVHASCAFVSAAAPWSLALHPSIG
                     YSIAAGFGALSFYRAKQGWQIVRYHQNLKRLPNYSMTSKQIPVSNKYLFVGKGFEWKP
                     IHTQRLHDCFSDNGSKYLEPSKLFKMAREYEKRHENWLTRLTSKDSRWNPVRPLPPVE
                     GIPAIHGIEPDEVDVVQSVSSRVGHTVVFGTTRVGKTRLAEVFVTQDIHRGKTPDERE
                     VVVFFDPKGDADMLKRMYAEAKRAGREDEFYVFHLGYPEISARYNPVGRFNRISEVAG
                     RISGQLSGAGNSAAFKEFAWRFVNIVARALVELGNRPNYVQISQFVQNIDSLFLDYAE
                     KFIRKTDPKIWENLATIAAGLDEKALPFGMKDRPLIFVINKYIAESAVFDPILQGLAS
                     AVRYDRTYFDKIVASLLPLLEKLTTGKISELLSPDYADILDERPIFDWESIIRKRGIV
                     YIGLDALSDATVAAAVGNSMFADLVSMAGHLYKFGTNEGLPGDMGRKTKLVKINLHCD
                     EFNELMGDEFIPLINKGGGAGVQVTAYTQTLSDIEARIGSKPKAGQTLGNFNTLYMLR
                     VKEPATAELLTKQLHEVTIKRSTVTSSTTDSSNPDDDKAFSSNTGQRISESTVPMLVP
                     ANITNLPKGQAFALLDGSKLWKIRMPLPAVDKDDMMPQSLRELTDYMRKNYHVVENWW
                     QPAFTEYTPSRDIAEAFEAMSEPQYNSRIDNQVDNFGIDEPYDIDEEENDDE"
     gene            5451..5804
                     /locus_tag="PXD66_000587"
     CDS             5451..5804
                     /locus_tag="PXD66_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
                     /protein_id="HDL6318917.1"
                     /translation="MSSTKMREENRTNLLDLPNKYRNFDGEFSASCGLDNAEELLIHS
                     QSYFIEWFQQGYSFHQFAEKFAHQGLSLWSADEISMRNTDKSKDVFAFYLAFDHNPSG
                     YILVQCQLDREDSLQ"
     gene            5801..6496
                     /locus_tag="PXD66_000588"
     CDS             5801..6496
                     /locus_tag="PXD66_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03747 family integrating conjugative element
                     membrane protein"
                     /protein_id="HDL6318918.1"
                     /translation="MSEGAKPRRKSGLISLILGKINMLLAAITVSLIFSIILEWVGIA
                     FWWPEEGHLHSQTMMLNEMQWLSDDFSQSLFAKSSTAFATLIIENVYNWLFVYTGIKE
                     WLTSPGNGSMEQWAYHYGRAYIESVIYVVITFIIRLIIIIFTSPLFLLTALSGLTEGL
                     SLRDIRKFGAGRESAYLYHHARRYIMPMMVTAWVVYLSIPFSIHPNFILVPAACLFGL
                     SICITAASFKKFL"
     gene            complement(6526..6885)
                     /locus_tag="PXD66_000589"
     CDS             complement(6526..6885)
                     /locus_tag="PXD66_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318919.1"
                     /translation="MRYLALFPVFLPSIAYPLPQAHKVGDICHAENAPYNYGGLWELS
                     LRGNLVCNYWQFLGDDERKRIESQLDEYGELPLWYSSERETEEDEYPQYWEVEDWDGD
                     DWDIETENEGTNTITDE"
     gene            7083..7409
                     /locus_tag="PXD66_000590"
     CDS             7083..7409
                     /locus_tag="PXD66_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAQPRD family integrative conjugative element
                     protein"
                     /protein_id="HDL6318920.1"
                     /translation="MSISTSLRKGIVVLVTLSCLPAFASTEQEQLAQAIKQLEAAKLS
                     LQRAEKVAKASPKTREYFNYSAIHRDIETVKKGIQQYINPSRALPRDPNVLRTLSEDY
                     SKLRGQ"
     gene            7409..7642
                     /locus_tag="PXD66_000591"
     CDS             7409..7642
                     /locus_tag="PXD66_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03758 family integrating conjugative element
                     protein"
                     /protein_id="HDL6318921.1"
                     /translation="MSYSPVDAFSAASGIDPFRLKLMIAIIFLSMLLLAVAWALNSGY
                     KGFSFRDKSPFSLLVLFLKGVALLVCVTYFFIY"
     gene            7657..8046
                     /locus_tag="PXD66_000592"
     CDS             7657..8046
                     /locus_tag="PXD66_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2976 domain-containing protein"
                     /protein_id="HDL6318922.1"
                     /translation="MSRIKSLTKHLHSRYLAVMLVFWGTIQHAFAAGPDASKIPDFQI
                     PGVDSNTKDPMEILWIVGKSIITLFAVLLAAGAIFVVVKALISIYNEATSENGKKGWG
                     HFIVALIIGFIVIFFCLWLVKLAVGLF"
     gene            8069..8434
                     /locus_tag="PXD66_000593"
     CDS             8069..8434
                     /locus_tag="PXD66_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03750 family conjugal transfer protein"
                     /protein_id="HDL6318923.1"
                     /translation="MPEQDTTIPFLPTRLNREATVYGGMTVSEFGISAGLGFMLGLIV
                     GLFLWILTDFWLLIPAMAMLLCIATVLIGKGVVAAVKRGKPEAYLNRLIEKKMDDLFN
                     GHKFIRREGFWSIRRYKRK"
     gene            8438..9082
                     /locus_tag="PXD66_000594"
     CDS             8438..9082
                     /locus_tag="PXD66_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03746 family integrating conjugative element
                     protein"
                     /protein_id="HDL6318924.1"
                     /translation="MSQLKSQLHEKDKHIKTTRIFAFLFFIIILVLAGVIYTFPSRLT
                     IYNPPDLRAGSQRAWWEIPKPTIYAFSYQIFQQMNRWMTNGEEDYEKNITALKPFLTP
                     HCEAFLRQDYKDRLRNNELRSRTRMVYEITGRGYSEDKVTVFSEDNWAVNLDLSVDEH
                     FQGQPVKRVYIRFPISIVRMDIDSVKNPWGLGFNCYHSIPKLLEGVEPKTEEKK"
     gene            9082..9963
                     /locus_tag="PXD66_000595"
     CDS             9082..9963
                     /locus_tag="PXD66_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03749 family integrating conjugative element
                     protein"
                     /protein_id="HDL6318925.1"
                     /translation="MKRLPILFASLLISFSGSSFADVLMKWERKPLPIALQVEKERII
                     FVDKNVKVGYPAELDGKLRIQSTGGAVYLKALETFPSTRIEFRDVSTGEIFLFDIVSA
                     TKVANPEESIRMVFDSAVEKQSFSTNRDDEPDLSLTGTRPQLPIPAALTRYAAQSLYA
                     PLRAVEPLEGVRRVAHRLPKKITTLLPAYSIEATPLISWQLDEYVVTAIRLQNKGNSQ
                     ITLDPRELQGQFYAATFQHNWLGGYGGNEDTTTLYLVTEGYANNALIPETHPVKPVGK
                     KVKKTTKVETKPTQSAK"
     gene            9975..11429
                     /locus_tag="PXD66_000596"
     CDS             9975..11429
                     /locus_tag="PXD66_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HDL6318926.1"
                     /translation="MKANKALFVILGIVFTLVGGIVYALFWRSDTPQEPTEQEILDIS
                     IKDLTPEQVRSMGIQGDTPIDTLRTLVTTVKSNERRMDELIDENRQLKQKEDLVNGRI
                     SDAVNNETRKLQEQIADLKMQLFDMATHVNNKSTAQESTPQAEAQPSMTTNKGFGTAG
                     DKLPIGGASMPNVDIGGMYWVEPSDQIGLDAGGKAVSGNTLATKFGFPNDFKGGQTTG
                     NTEKKGLPFFPETDVVTGSQGTTGNSRGKNQITPVYTVPSNSTFLDSVAMTGLIGRIP
                     IDNKVSDAYPFKVLIGRENLIANGIELLDVEGAIVSGTATGDWTLSCVRGDVKSMTFV
                     FNDGRIVSTTNKGNSENIGWLSDSSGIPCVPGERKTNAPEYLGTNFFLAAASAAAQGF
                     SQSQTTTVVDGNSVVGAVTGDQGKFILGQAIGGGLKESADWFRKRFAENFDAVYVPPG
                     HRVAIHIEKEIEIDYNSKGRKVKYGNTSTNTQLD"
     gene            11442..11840
                     /locus_tag="PXD66_000597"
     CDS             11442..11840
                     /locus_tag="PXD66_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HDL6318927.1"
                     /translation="MKALYCIMMSALLITGCTTSTEELLPANGSTMRDIWDKGAGNND
                     LHLYRSQQGRTLDPVDYIPNTEQRAYTRTAENEINNLFPRLPNPDLIMYVYPHQTRSG
                     EPLSIPGYSTVLPFYGRVQYAQPGDRTRGL"
     gene            11840..14677
                     /locus_tag="PXD66_000598"
     CDS             11840..14677
                     /locus_tag="PXD66_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HDL6318928.1"
                     /translation="MMSFLSEVFSSFKPTKTEVISSEPNENSGESDIIQNKMSEVNGE
                     VQPLKRSGKLTLSQHHRRLYSQAPSFIDHLPWGEYLNQHGVILLDDARSVGAVFDIKP
                     IGTAGRSIEFLSRIRNLVKDALQDSFDELDINPYVVQFYCQDSDDLQPYIDQLRNYIS
                     PIAQNSAFSEEWLRVQAKHFKDIGKPEGLFIDDRVTNTAWSGRIRSTKMVIYRYVDKS
                     NDSNENLIIEQLNNACSRINGALTTAGLQLQRLDGEQIRQWLLRWFNPNPSINFEGLS
                     NNDCYHLFGKQTSNDDLPLAFTDFSEDLFYSQPRNEGSYWYFDGQPHEVVVVNNLRKQ
                     PEVGLMTGEITRGRNINALFDLLPESTIMAITIVIHPQDKLEAHLEKISSRAVGENFE
                     SKYLKEDCQTVQAYLKEGHKLYQAAIAFYVRGKDEKELRTRSRQLRTILLSNNLEPVK
                     ENAEIAPLNSYLRWLPMNFNPQMDAKSHYYTKYYFVQHLANLLPIFGRDTGTGNPGIT
                     YFNRGGAPFNFDPFNRKDRTKNAHKLLLGPTGSGKSATLCGQLCQAMAVYRPRTFVVE
                     AGNSFGLLADYAERFGLTVNRITLAPNSGIVLPLFSEAHRLLDMELNPEDVPEDIEDN
                     DDTDDEGDMQRDLLAEMELTARLMITGGEKKEDEKMSRADRAAIRRAILNAARQTKSE
                     GRQTLTEDVKKALEAISRDENTREERRTRLAEMAESLEMFCTGVNGQFFNREGEIWPE
                     ADLTLVDLAMFAREGYEAELAISYISLINHINSLGEKYQHSDRMILNVTDESHIITAN
                     PLLAKFLVKGSKMWRKLGIWLWLATQNMDDFPNEAAKLLSMVEWWELLNVSASEIDEI
                     NRFRKLSEAQKTMLLSAKKSDRKYTEGVVLATNMEALFRVVPPSLFLALGMTEKHEKA
                     ERRQLMMQHGYSELDAALEVAARIDKARGIA"
     gene            14677..15105
                     /locus_tag="PXD66_000599"
     CDS             14677..15105
                     /locus_tag="PXD66_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318929.1"
                     /translation="MMKQHYRLFNVGPQKSSLGIVSSAVETQLLGFCEQIRQLIGIGK
                     QITLFPHRVVQNHWAIELSGEKGSVHLLLNVTGRSLLPTSNYREWPARLVIEIADHVD
                     AYWFATFLAQQLDLKSNLFFLSTSIVDNSMHNEVTDTNEA"
     gene            15095..15412
                     /locus_tag="PXD66_000600"
     CDS             15095..15412
                     /locus_tag="PXD66_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
                     /protein_id="HDL6318930.1"
                     /translation="MKPDTTMPKMKKVRAVLKTTLIIIGIILLLLIVGWLAMTYFPTT
                     NFQAWFYETRYAWLGWRFVLYGVIWFLMVKLNRRGQMPLKARLMILSAIPLIEGINLL
                     YLL"
     gene            15423..16940
                     /locus_tag="PXD66_000601"
     CDS             15423..16940
                     /locus_tag="PXD66_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HDL6318931.1"
                     /translation="MTFTVDSYLEYFLTLLAWIINNNIFAVLVQTGLFILPLIFVLIG
                     TWMEVKKQGEDEGNKGDLLITWLSLKFYPAMFVVVLVLAPMIPINLNNIELNVERSKA
                     CGYRIPTSPEDSGYNQMNSELGNQQARVPLWWGLFHQINKGVTHALTASIPCKPDLRQ
                     VRFEVQHEKINNPALLTEVRLFVQQCYVPARQKIQSSQLSLTPAQVRETSWLGGRILL
                     TNSELYPRYRAQQPHSLFPYNASRDNGLPNNGEGGFPNCAEWWDTANTGLKDRLLADV
                     KQNFSAKVIDFFSSKENADESILRTVLRAENMDISRGKVYPGYGGNLDPTFANATTRL
                     TSSVGSLAASFAAFPAFDAMRQALPMVHAFMLMAVIIITPLVIVMSGYSLKTLVTLTF
                     VSFALVSLTFWWELARWLDSWLLSALYDSSAHSRINPYFLENTQDDFIVNFVMGSMFL
                     VLPAIWLGSMSWAGVKIGDIAAGFTTDSLDSKSAGSQGGNLVKKGGKEISAGFNK"
     gene            complement(16993..17373)
                     /locus_tag="PXD66_000602"
     CDS             complement(16993..17373)
                     /locus_tag="PXD66_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318932.1"
                     /translation="MKFIDFGKQLAKPVKFIKQRTAFLPKPLQQAVVFAVIFAGLPLF
                     LVGFTVLRILNGTPVEDISTNDVAYNPNHVENTVNEIEDDSEDSKPEDLWDVIADQDE
                     NQLDGYRDGYFGYGLYQGGYFVKR"
BASE COUNT         5351 a         3351 c         3665 g         5093 t
ORIGIN      
        1 tttcaagata ttgaagatta catgatacat gagggatatt tacatggtat acctcagact
       61 tggaaacatt tggtttcaca tagcgaacaa tggcatcaac acatattaga agagcaacga
      121 agagatcaac acatattaga agagcaacga agagaaaatg atgaggctca ccaagcactt
      181 ttatctacat cttggtcatc tccaattgat atttgctata tcaatgaaat tgaatttcaa
      241 cctttgacga cgggacaaat gttatttgat gaaggacaac gtatgaggca ttgtattttt
      301 acatacatag atatctgctc agaggggaga tacttagctt tctccgtaaa aatagccaat
      361 gaatcagcta ctttaggatt aataaaacat agaggaaact ggaaaattga acaatttcgt
      421 tatgcctgta atattgcagt ttgtgagcct atgattttag cagcagctca aaaactatgt
      481 aaattactta acaaacaatc agccttacct caaaattaat tttatctccc aatcaacaat
      541 agaataaatc attcaaaatc agcaatgact tcgccatagt tggcttagat tcctttcaca
      601 cttggagaga ttaaatgaaa acaaaaatat ccttaattgc tgtttttatg cttggtttaa
      661 gtggttgtgt tcatcaacaa aatactgctc aaaacagtga tgaaatcaca cctatgcctg
      721 attcattact aaaacctcaa atgcaaccat tacctccaca agaccgtatc gtgtccaaag
      781 atatctacca cgaacaaact acctcgaata ccgaagtagt acgtgacgga cgttacacct
      841 taatcagtat tgctcctgag gaaggacaaa aatatctgct tgaacaagtg gtatcggtaa
      901 aagtgcctaa tactaaaaat agattcacag ccaatgttca gcaaggcctc cgtacaacat
      961 taaccaatac agggttagaa ctgtgctttc caccaatagg tgaacagggc aatgcagttc
     1021 aaacgctttt tactcgccca cttccgaaag tccattacca attcggtccg atgaaattat
     1081 atgaagctct acaaatgatc gcgggaccgg cttatgaatt aacacttaat gatattactc
     1141 gtacgatttg ctttaaagct cgggaaagct cattccaaaa tcctcataag cctgctgttg
     1201 ctattcaacg gcaacaatta aaaccgaggt cattgaggaa taattgaggg aaactccaat
     1261 gaaacgaaat actatcaata tatggttagc cgtactctct tcagtgacta caaatgctct
     1321 tgctcaaact acatcaatag aaagtagcac acagacctta tcgcaacagc aaagccaaca
     1381 tcaacaatca caattaagtg atagcctaaa acaggcaaaa gagtggggat taaccattga
     1441 agaatggaat cgctacaccg aattaatgaa aggtgagcgt ggtatgtggt cgccaaactt
     1501 agatcctctt acagcattag gtattgaagc aagaactgct gaggagagag aaaaatatgc
     1561 tcgtatgtta gcaaaaaaat actatgaacg tgtgtccaaa gaactggatt ttgacaaagt
     1621 ctatcgtcag gagtttgaga aactgtatcc aaatgaactt ccttttgaag tagaaccaca
     1681 catttcacaa tccataggtc gagtgattta ttttacacgt tttgataatt gcgattcttg
     1741 taaaagtgat ctcagtaaaa tcctctctta tgccaatagc caaacaccgc ttgatatcta
     1801 tattgtaggg caaaacattt ctgataatga tatccgcaaa tgggctgctg agaataatat
     1861 tgatccgatt aaagtgagta agaaactgat tacacttaat catgataagg gttattggtt
     1921 ccaatattca ttaggtaaaa tgccggcagc gtatcaaata aaacaggatg gtgaatggca
     1981 atctctcgtt tattaatcgt tatcacgcta tttgcggtcg ggataagtga tgctaagtca
     2041 aaacctttta ccggatttgg tacagaaatt gatagctata tccgtatagc agctcagcgt
     2101 tatcaaatta gcgaaccgat gctaagaggg ctaataaaaa tggaagatgg ttggtatggc
     2161 aatatctcac ctactggtgc cacaggtgta gggcaattta ctgtgggaac gtggaattgg
     2221 ctatctgaaa cagaacgtgg taagcagctt ggtatgcgtt taattaccgg taagaataaa
     2281 ggtacttatg ctgatcctcg cagagataaa tacaccaata cgttagcaac agcgttatta
     2341 gctcgatggc atattgaaca attccattta cgtcgcattc cggcaacaga tgaaaattta
     2401 tatatggctc ataacattgg tttagatagt ctgcatcgag caatgatggg aaaagctacc
     2461 aaagaagata tcttaaatat gcgtcgcaat ggaatgaaaa gttggatgac agtaagagat
     2521 ttcctcaatt atcaaaaagg acgcttttta gcacataaac aagaagccaa ttttatgttg
     2581 gtctcaaaac aggggcatat tcatactggt gtttccgcct ttgtttatcc tacaagtgaa
     2641 atgggaaaat cagtaaagtg ggttgagcca gacaacaaaa taatttgggt tgaacctaaa
     2701 agtgcaatga tttgggtaga acctacaaat acgccataga ggtaaaaatg caacataaga
     2761 taaaaatgct attgctcagt ttatgtatat ctcattccgt ttggggcgat ttaacggtaa
     2821 tcgctgattt cggcggtgaa agtgcggtac gtttttacga agctttacaa ccggaacacg
     2881 atgagaatgc accgaactat ccgaattcta ttccagcttc ggttagcgaa gctgatattc
     2941 tacctgtagt ctctcataaa ctgtctcctg gtattattga aacaaaacaa tttgatttaa
     3001 caggaataca agcgattttt ttaattggag cagataatac ctcagctcaa tggttacagc
     3061 agaatcgaga aaagttgatc acgttaaatg ccactgggct tgttgtgaat gtccgaacat
     3121 tagcagagct gaatcaatta cgagaaattg tgccagaaat aataatgcta ccaatccccg
     3181 gagacgacct tgcagaacga attggtatcg cacattaccc tgccctgatt actgaaaccg
     3241 gagtttcaca ataatgtcag ctaaacatac cttggaggcg tggttacgcc ctcctgttga
     3301 gttttttccg gcaattgttc atgcttcttg tgcttttgtc agtgctgctg cgccttggtc
     3361 tctagcactc catccaagta tcggctatag tattgcagcc ggtttcggtg cgttgagttt
     3421 ttatcgagca aaacaaggat ggcaaattgt tcgttaccat caaaatctga aacgtttacc
     3481 gaattattca atgacgagta aacaaattcc ggtcagtaat aagtatctat ttgtcggcaa
     3541 gggatttgaa tggaaaccta ttcacactca acggctacac gattgttttt cggataacgg
     3601 ttcaaaatat ctggagcctt ctaagctatt taaaatggct cgtgagtatg aaaaacgcca
     3661 cgaaaattgg ctaacacgtc ttacctcaaa ggatagccgg tggaatccgg ttagacctct
     3721 gccaccggtt gagggtattc ctgctatcca tggaattgag ccggatgaag ttgatgtcgt
     3781 tcaatccgtt agctctcggg ttgggcatac ggtagtattc ggtacaacac gtgtcggtaa
     3841 aactcgttta gctgaggtct ttgttaccca ggatatccac cgaggcaaaa caccggatga
     3901 gcgtgaggtt gttgtctttt tcgatccgaa aggcgatgcc gatatgctca aacgaatgta
     3961 tgcagaagca aagcgagctg gaagagaaga tgagttttat gttttccatt tgggatatcc
     4021 tgaaatttcg gctcgctata acccagtggg acgttttaat cgtatatcag aagtcgctgg
     4081 acgtatttca ggacaactca gtggagccgg taactcagcg gcatttaaag agtttgcttg
     4141 gcgatttgtc aatatagttg cacgtgcatt agttgaatta ggaaatcgtc caaactatgt
     4201 tcagatttcc caatttgtac aaaacattga ttcattattc cttgattatg cggaaaaatt
     4261 tattcgtaaa actgacccta aaatttggga aaatttagcg acaatagccg caggattaga
     4321 tgagaaagct cttccttttg gaatgaaaga tcgccctcta atttttgtga tcaacaaata
     4381 tatcgctgaa agtgcagtgt ttgacccgat tttacaagga ctcgccagtg ccgtgcggta
     4441 tgaccgtacc tattttgata aaattgttgc ttcgttattg ccgttattgg agaaattaac
     4501 gaccggaaaa atttccgaac ttttatctcc ggattatgcc gatattttgg atgaacgccc
     4561 tatttttgac tgggaatcca ttatccgtaa acgaggaatt gtttatattg gtttggatgc
     4621 tctctccgat gctacagttg ctgccgcagt cggaaacagt atgtttgcgg atttagtatc
     4681 aatggcaggt catttgtata aatttgggac aaatgaggga ttgccgggtg acatgggcag
     4741 aaaaaccaaa ctggtgaaaa tcaatcttca ttgtgatgaa ttcaatgaat tgatgggcga
     4801 tgaatttatc cctctcatca acaaaggtgg tggtgccgga gtacaagtta ctgcttatac
     4861 gcaaacgctc tccgatattg aagcccgtat tggcagtaaa ccgaaagcgg gacaaacctt
     4921 aggtaacttt aataccctat atatgctcag ggtaaaagaa cccgctacgg cagaattgct
     4981 tacaaaacag ttacacgaag tgacgattaa gcgtagcacg gtcacgtcca gtacgactga
     5041 ctccagtaac ccggatgatg acaaggcatt tagctcaaat acagggcagc ggattagtga
     5101 aagtactgtt cctatgctgg ttcctgccaa cattaccaat ttaccaaaag gacaagcatt
     5161 cgctttactg gatggtagta aattatggaa aattcggatg ccgttaccgg ctgtcgataa
     5221 agatgatatg atgcctcaat ctctacgaga gctgacagat tatatgcgta aaaactatca
     5281 cgttgtcgaa aattggtggc aaccagcatt tacagaatat acgccgtccc gagatattgc
     5341 agaagctttt gaagcgatgt cagagcctca atataactca cggatagaca atcaggtcga
     5401 taatttcggc atagatgaac cctatgatat tgatgaagag gaaaatgacg atgagtagta
     5461 ccaaaatgag agaagaaaat agaaccaacc tcttagattt acccaataaa taccgtaatt
     5521 ttgatggcga gttttcagct tcttgtggac tagacaatgc cgaagagctc cttattcata
     5581 gccaatccta ttttatcgag tggtttcaac aaggctactc tttccatcaa tttgctgaaa
     5641 aatttgcaca tcaaggactt tcattatggt ctgctgatga aatatctatg cgtaatactg
     5701 ataaatctaa agatgttttc gctttttatc ttgcatttga tcataatcct tctggctata
     5761 ttcttgttca atgtcagctc gatcgggagg atagtctgca atgagtgaag gagctaaacc
     5821 tcgtcgaaaa tcaggtttaa tcagcctgat tttgggaaaa ataaatatgt tgcttgctgc
     5881 aattacagta tctctcatat tcagcattat tttagagtgg gtaggaattg cgttttggtg
     5941 gccggaagaa ggacacttgc atagccaaac gatgatgctc aatgagatgc aatggctatc
     6001 agatgatttc tctcaaagcc tgtttgccaa atcttctact gcttttgcaa cattgattat
     6061 cgaaaatgtc tataattggt tatttgttta tacgggtatt aaggagtggc taacttcacc
     6121 gggtaacggt agtatggaac agtgggcata tcactatggt agagcctata ttgagtctgt
     6181 tatttatgtc gtgatcacct ttattattcg cctcattatt attattttta ccagtcccct
     6241 ctttctattg accgcacttt ctggtttaac cgaaggatta tcattacggg atattcgaaa
     6301 atttggtgcc gggcgggaat cagcctacct ataccaccac gctagacgtt atattatgcc
     6361 gatgatggta acagcttggg tagtttattt gtctatcccg ttttcgattc accctaactt
     6421 cattttagtc cctgctgcct gtttatttgg tttgtccatc tgtataactg cagcgagctt
     6481 caagaaattc ttgtaaagat aataagcctc agtaatatac tggggctatt cgtctgttat
     6541 agtgtttgtc ccttcatttt ctgtttcaat atcccaatca tctccatccc aatcttcgac
     6601 ttcccagtat tgtggatatt catcctcttc ggtctctcgt tcagagctat accaaagagg
     6661 caattcccca tattcatcca attgtgactc tatgcgtttt ctctcgtcat cacccagaaa
     6721 ttgccaataa ttacagacta aatttcccct caaacttaat tcccaaagac caccgtaatt
     6781 atatggggca ttttctgcat ggcagatatc tcccacttta tgtgcctgcg gaagagggta
     6841 agcaattgat gggagaaata cagggaatag agctaaatat ctcatctggt atctcatggt
     6901 aaagcgtgaa tactcaactt taatactaac ttattaagta acactgacac agttgaatat
     6961 ctttctaacc atttaatatt ttgtgcaatt tctcttctga ttggtaacag agttttccat
     7021 taaattttag gatcgctttt ggcactctta acttgaagta aaactaaaaa tagaggtgca
     7081 ctatgtctat ttcaacatct ttaaggaaag gtattgttgt tcttgttacc ttatcttgct
     7141 tgccggcatt tgcttcaaca gaacaagagc aattagctca ggcaattaag caactggaag
     7201 cggctaaatt atcactccaa cgagcagaaa aagtggctaa agcctcgcct aaaacacgag
     7261 aatacttcaa ctattctgcc attcaccgtg atattgaaac cgtcaaaaag ggaattcaac
     7321 aatatatcaa cccaagcaga gcattacctc gtgatccgaa cgtactacgt actttaagtg
     7381 aagattacag caaactacgg gggcaatgat gtcctattcc cctgttgatg cattttctgc
     7441 agcgagtggt atcgatcctt tcagattgaa actcatgata gctattattt tcctttcaat
     7501 gctgttacta gcagtcgctt gggccttaaa tagtggctat aaggggtttt catttagaga
     7561 taaaagtcct ttttcacttt tagttttatt tctcaaagga gtagcactat tagtttgtgt
     7621 tacttatttc tttatctatt agattcgagg tctattatga gccgtattaa atctcttacg
     7681 aaacatctgc atagtcgtta tttagctgta atgctcgtat tctggggaac tatccaacat
     7741 gcgtttgcag ccggtccgga tgcatccaaa atcccagact tccaaattcc aggcgtggat
     7801 agtaatacta aagatccaat ggaaattctt tggattgtag gtaaaagtat cattacctta
     7861 tttgcggtat tacttgccgc cggtgccatt tttgttgtcg ttaaagcctt gatcagtatc
     7921 tataacgaag caacttctga aaacggcaag aaaggctggg gacactttat tgttgcttta
     7981 attatcggct ttattgtgat tttcttctgc ttgtggttag tcaaacttgc agtcggactc
     8041 ttctaattaa aatgtctagg agaccaatat gccagaacaa gatacaacca ttcctttttt
     8101 gcctactcgc ctcaatcgag aggctaccgt ttacggcggt atgacagtga gcgaattcgg
     8161 catatctgct ggattgggat ttatgttagg tctgattgtt ggtctcttct tgtggatatt
     8221 aactgatttt tggcttctta ttccggcaat ggcaatgttg ctctgtatcg caaccgtttt
     8281 aattgggaaa ggtgttgtag ccgctgttaa gcgaggaaag cccgaagcct acttaaatag
     8341 gttaattgaa aagaaaatgg atgacctctt taacggtcat aagtttataa gacgagaagg
     8401 gttttggtct attcgccgat ataaaaggaa ataacaaatg agccagttaa aaagccaatt
     8461 acacgaaaaa gataaacata tcaaaacgac tcgaattttt gccttcctgt ttttcattat
     8521 tattttggtg ctcgcaggcg tgatctacac attcccaagt cgccttacga tatacaaccc
     8581 accagatcta cgagcaggga gccaacgagc ttggtgggaa attcctaaac caacaattta
     8641 tgcattttct tatcaaattt tccagcaaat gaatcgttgg atgacaaatg gcgaagagga
     8701 ttacgagaag aatattaccg ccctcaaacc atttttaaca ccacactgtg aagcattttt
     8761 acgacaagat tataaagatc gtctccgtaa caatgaacta cgtagccgaa ctcgtatggt
     8821 atatgaaatt accggtcgag gctacagtga agataaagtg accgtattta gcgaagacaa
     8881 ttgggccgtc aatcttgatt taagcgtaga tgaacatttc caaggacaac cggtaaaacg
     8941 agtctatata cgttttccta tcagtattgt gagaatggat atcgattcag tcaaaaatcc
     9001 ttggggatta gggtttaact gctatcactc cattccaaaa ctcttagagg gcgttgagcc
     9061 aaaaacagag gagaaaaaat aatgaaacgt ctaccaattt tattcgcttc tttactaata
     9121 agcttctctg ggagtagctt tgctgatgtc ttgatgaaat gggaaagaaa acccttgcct
     9181 attgcattgc aagtggaaaa agaacgaatc atttttgtcg ataaaaatgt caaagttggc
     9241 tatccggctg aattagatgg caaattacgc attcaatcaa cgggtggcgc agtgtactta
     9301 aaagccctag agacattccc ttctacccgt attgaatttc gtgatgtttc tacaggtgaa
     9361 attttcctct ttgatattgt ttctgcaacc aaagtagcta atccggaaga atcaatacga
     9421 atggtatttg atagtgcggt agaaaaacaa tctttttcaa cgaatagaga tgatgaacct
     9481 gatctatctt tgaccggcac acgaccacaa ttaccgatac cggcagcatt aacccgttat
     9541 gccgcacaat cactttatgc gccactgcgt gcagttgagc ctttagaagg agttcgtcgt
     9601 gtagcacatc gacttccgaa aaaaattacc acattgctcc ctgcgtattc gatagaggca
     9661 acacctttaa tcagctggca attggacgaa tatgtagtca cggctattcg tctacaaaac
     9721 aaaggtaata gtcaaattac actcgatcct cgagaacttc aaggacaatt ttatgctgca
     9781 actttccagc ataattggct tggaggatat ggagggaatg aagataccac aaccctgtat
     9841 ttagtgacgg aaggctatgc taataatgcg cttattccgg aaactcaccc tgttaaaccc
     9901 gtcggtaaga aagtgaaaaa aacaacaaaa gttgaaacta aaccgactca atcggcgaag
     9961 taggaggaga agctatgaaa gcgaataaag ccttgtttgt catattgggg attgtgttta
    10021 ccctagtcgg tgggattgtc tatgccttat tttggcgaag tgatacaccc caagaaccaa
    10081 cagagcagga aattttagat atttccatta aagatttaac accggaacaa gtcagatcaa
    10141 tgggcattca aggtgatacg ccgattgata ctctgcgtac tcttgtgaca accgttaaat
    10201 ccaatgagcg tcgtatggat gaattaatcg atgaaaaccg acaactcaag cagaaagaag
    10261 atttagtgaa tggtcgtatc agtgatgcag tgaataatga aactcgcaaa ctacaagagc
    10321 agattgcaga tttgaaaatg cagttatttg atatggcaac acatgttaat aacaaatcga
    10381 cagcacaaga atcaactcct caagcagaag ctcagcctag tatgacaacg aataaaggat
    10441 ttggtactgc cggagataaa ttacctatcg gtggtgcttc tatgcctaat gtggatattg
    10501 gaggaatgta ctgggtagag ccaagcgatc aaattggatt ggatgcaggt ggaaaagctg
    10561 tatcaggtaa tacacttgca actaagtttg gattcccgaa tgattttaaa ggtggacaaa
    10621 ctactggaaa taccgaaaag aaaggtctgc cgtttttccc ggaaactgat gtagtgacag
    10681 gctcgcaagg aacaacagga aattctcgtg gtaaaaacca gattacgccg gtttacaccg
    10741 taccaagtaa ttccacattc cttgattcag ttgcaatgac agggcttatt gggcgtatcc
    10801 cgattgataa taaagtctct gatgcttatc catttaaagt actgattggt cgtgagaatc
    10861 tcattgccaa tggtattgaa ttacttgatg tagaaggtgc gattgtttcg ggtacagcta
    10921 ctggcgactg gactctatct tgtgtacgag gcgatgtaaa aagtatgacc tttgtgttca
    10981 atgatgggcg tattgtcagc acgactaaca aagggaatag tgagaatatc ggttggttat
    11041 ctgatagttc aggcattcct tgtgttcccg gggagagaaa aacaaatgca ccagaatatt
    11101 taggcacaaa tttcttctta gcggctgcca gtgccgcagc ccaagggttt tcacaatcac
    11161 aaacaacgac agtagttgat ggcaatagtg ttgttggagc tgttaccggt gatcaaggta
    11221 agttcatctt agggcaagcc atcggcggtg gtttaaaaga atccgccgac tggtttagaa
    11281 agcgttttgc tgaaaacttt gatgctgtct atgtaccacc tggacatcgt gtcgctattc
    11341 atatcgagaa agagattgag atcgattaca actccaaagg acgtaaagtg aaatacggaa
    11401 acacatcaac taatacacaa cttgattaaa aggaacaaac aatgaaagct ttatattgca
    11461 taatgatgag tgccttattg ataacaggtt gcacaacttc aactgaagaa ttattacccg
    11521 ctaacggcag tacgatgcga gatatttggg ataaaggagc aggtaataac gatctacact
    11581 tataccgttc acaacaagga cgaacactcg atcctgtcga ttatattcct aacactgagc
    11641 aacgagccta tacccgtacg gcagaaaatg aaattaataa cttgttccct cgtttaccta
    11701 atccggatct gattatgtat gtgtatccac accaaactcg ttccggagag cctcttagta
    11761 ttccaggata ttctacggta ttacctttct acgggcgagt ccaatatgct caaccgggtg
    11821 atcgaacaag agggctttga tgatgtcttt tttaagtgag gtattttcct ctttcaaacc
    11881 gactaagaca gaggttatat caagtgaacc aaatgaaaat tcaggtgagt ctgacatcat
    11941 tcaaaataaa atgagtgagg taaatggcga ggttcaacca ttaaaacgca gtggaaaact
    12001 caccctgtca cagcatcacc gtcggttata ctcacaagcc ccctctttta ttgatcatct
    12061 accttgggga gaatatttga atcaacacgg tgtgatactc ctcgatgatg ctcgtagtgt
    12121 cggggcggta tttgatatca agccgattgg tacagcagga cgtagtattg agtttttaag
    12181 caggatacga aatcttgtca aagatgcctt acaagatagc tttgatgagc tggatataaa
    12241 tccttacgtc gtgcaatttt attgccaaga tagcgatgat ttacagcctt atattgatca
    12301 gctacggaat tatatctcgc ctattgccca aaatagtgca tttagcgaag aatggttacg
    12361 agtacaagcc aagcatttca aggatattgg taaaccagaa gggcttttta ttgatgacag
    12421 agtgacaaat acagcttggt ctggacgtat tcggagtacc aaaatggtga tttatcgata
    12481 tgtagataag tccaatgatt ccaatgaaaa tcttattatt gagcagctta ataatgcttg
    12541 ttcccgaatc aatggggctc taacaacggc tggattacag ttacagcggc tagatggtga
    12601 acaaattcgc caatggttat tgcgttggtt taatcctaat ccatctatta attttgaggg
    12661 attaagcaac aatgattgtt accatctttt tggcaaacaa acctcaaatg atgatcttcc
    12721 attagctttt actgatttca gtgaggattt attttattca cagccgagaa atgaaggtag
    12781 ctattggtat tttgacggac agccacacga ggttgttgtc gtcaataatt taagaaaaca
    12841 accagaagtg ggcttaatga ccggcgagat tactcgtggg cgtaatatta atgcgttatt
    12901 tgacctattg ccagaatcaa ccatcatggc aattaccatc gtcatccatc ctcaggataa
    12961 gttggaagca catttagaaa aaatcagtag tcgagcagtg ggtgagaatt ttgagtcaaa
    13021 atacctaaaa gaagactgtc aaactgtaca ggcttatctt aaagagggtc ataaactcta
    13081 tcaagcagct attgcatttt acgttcgagg taaggacgaa aaggaattgc gtactcgatc
    13141 tcgccaacta agaacaattc tgctcagtaa caatttagag ccggtaaagg aaaacgcaga
    13201 aattgctcct cttaatagtt acctacgttg gctaccaatg aattttaatc cgcagatgga
    13261 tgccaaatcc cattactaca cgaagtacta ttttgtacaa catttggcaa acttattgcc
    13321 tatctttggt cgagatacag gcactggtaa tccggggatt acttacttta atcgaggagg
    13381 agctccgttt aatttcgatc cgtttaaccg taaagacaga accaaaaacg ctcacaaact
    13441 cttattagga ccaacaggct caggaaaatc agcaacacta tgtgggcaat tatgtcaggc
    13501 tatggccgtt tatcgtcctc ggacgtttgt tgtggaggca ggaaattcat ttggtttatt
    13561 agcggactat gcagaacgtt ttggactgac cgtaaatcgc attacgttag ccccaaacag
    13621 tggcattgtc ttaccgctct tttcagaagc ccatcggtta ttggatatgg aattaaatcc
    13681 ggaagatgtg ccggaagaca ttgaggataa cgatgacact gatgatgaag gcgatatgca
    13741 acgagatttg ttggccgaaa tggagctgac agcacgttta atgatcactg gtggcgaaaa
    13801 gaaagaagat gaaaaaatga gccgtgctga cagagcagcc attcgacgtg ccattttaaa
    13861 tgcggctcgc caaactaaat ccgaaggcag acaaacgcta actgaagatg tgaaaaaggc
    13921 attggaggct ataagccgtg atgaaaatac tcgtgaagaa cggcgtactc gtttggccga
    13981 aatggcagaa tcattggaaa tgttctgtac cggagtcaat ggacaatttt tcaaccggga
    14041 aggcgaaatt tggccggaag cagatctgac attggttgat ctagccatgt ttgcccggga
    14101 aggttatgaa gcagaattgg ccatttccta tatttcactc attaaccata tcaacagtct
    14161 cggtgaaaaa taccagcata gcgacaggat gattctgaat gtaaccgatg aatcacacat
    14221 tatcactgcc aacccattat tagcgaaatt cttggtgaaa ggctcaaaaa tgtggcgtaa
    14281 attagggatt tggctgtggc ttgcgacaca gaatatggat gacttcccca atgaagcagc
    14341 gaaattgctc tcaatggtcg aatggtggga actgttgaat gtgtctgcta gtgaaattga
    14401 tgaaatcaat cgcttccgga aactgtccga agctcagaaa acgatgttgt tatctgccaa
    14461 aaaatcagat cgtaaatata ccgaaggtgt ggtattggca acaaatatgg aagcgctatt
    14521 ccgtgttgta ccaccgagtt tatttttggc tttaggaatg accgagaaac acgaaaaagc
    14581 agaacgtcgg caattaatga tgcaacatgg ttattctgaa ttagatgctg ctttagaagt
    14641 cgctgctcgt attgataaag cgaggggaat tgcataatga tgaaacaaca ctatcgacta
    14701 tttaatgtcg gaccacaaaa atcctcatta gggattgtat cttcggcggt agaaactcaa
    14761 ctgcttggat tttgtgaaca aattcgccaa ctcatcggta tcgggaagca gattacctta
    14821 ttccctcatc gggtagtgca aaaccactgg gctatcgaac ttagcggcga gaaaggctct
    14881 gttcacttat tactaaatgt gacggggcgt tctttactgc ctacttccaa ttaccgtgaa
    14941 tggcctgctc gtttagtcat cgaaatagcc gatcatgtgg atgcttactg gtttgcaaca
    15001 ttccttgctc agcaattgga tttgaaaagt aatcttttct tcttatccac atcaattgtg
    15061 gataactcta tgcataacga ggtaacggat actaatgaag cctgatacga caatgcctaa
    15121 aatgaaaaag gtgagagctg tattaaaaac aaccttgatt atcatcggta ttatcttact
    15181 actacttatt gttggttggc tggcaatgac ctatttccct accacaaatt ttcaagcgtg
    15241 gttctatgaa acccgttatg catggttggg atggcgattt gtattatatg gggttatttg
    15301 gttcttaatg gtaaaactca atcgaagagg acaaatgcca ttgaaagcca gactgatgat
    15361 tttatctgct atccccctta tagagggaat taatttactg tatctacttt aaggaggatg
    15421 agatgacctt taccgtagat agctacttag aatacttttt gaccttacta gcttggatca
    15481 ttaacaataa tatttttgct gtactggttc aaaccggtct attcatacta ccgctgattt
    15541 ttgttttaat cggcacatgg atggaagtta aaaagcaagg ggaggacgaa ggtaataaag
    15601 gagatttact tattacttgg ctcagcctta aattttatcc ggcgatgttt gttgtagtgt
    15661 tagttttggc cccgatgata cctatcaacc ttaacaatat tgagctgaat gttgaacgtt
    15721 ccaaagcctg tggctaccgt attccaacct caccagagga tagtggttat aatcaaatga
    15781 actctgaatt gggcaaccag caagcgagag taccgctttg gtggggatta ttccatcaaa
    15841 tcaacaaagg agttacacac gcattaacag cttcaattcc gtgtaaaccg gatttacgtc
    15901 aagttcgctt tgaggtccaa catgaaaaaa tcaataatcc tgcattattg actgaggttc
    15961 gcttatttgt acaacaatgt tatgtacctg cacgtcagaa aattcaaagc agtcagctct
    16021 cattgactcc ggcacaggtg cgtgaaacca gctggttagg tggtcgtatt cttttgacaa
    16081 acagtgagct ttatccccgt tatcgagctc aacaacctca tagtctattt ccctacaatg
    16141 cttcccggga taatggattg ccgaataatg gggaaggtgg ttttccaaat tgtgcagagt
    16201 ggtgggatac ggcgaatact ggtttaaaag atcgtttatt agctgatgtc aaacagaact
    16261 tctctgcaaa agtgattgat ttttttagca gcaaagaaaa tgcggatgaa tccatactcc
    16321 gtactgtatt acgagcagag aatatggata tttcgagagg gaaagtttat cccgggtatg
    16381 gggggaattt agatcctaca tttgcaaatg caacgactcg attaacatct tcagtgggaa
    16441 gcttagctgc cagctttgca gcctttccag cattcgatgc aatgcgacaa gcactgccga
    16501 tggtacatgc ttttatgttg atggctgtga tcatcattac tccgctggtt attgtgatga
    16561 gtggttattc tctcaaaaca ttagttacat taacttttgt gagttttgct ttggtatcac
    16621 tgacattctg gtgggaatta gcaagatggt tagattcttg gctactatcc gctctatatg
    16681 attccagtgc acatagtcga ataaatccgt atttccttga aaatacgcaa gatgatttta
    16741 tcgtcaattt tgtgatgggt tcgatgttct tggttttacc ggcgatttgg ctaggcagta
    16801 tgtcgtgggc tggagtaaaa ataggagata tagctgccgg ttttactact gattcattag
    16861 atagtaaatc cgcaggctct caaggtggta atttagttaa gaaaggcggt aaggaaatat
    16921 ctgcagggtt taacaaataa aacaataagg tagaggttaa tctctacctt attaatttct
    16981 aatcatattt tctcatcttt ttacaaaata gccaccctga tagagtccat atccaaaata
    17041 tccatcacga tatccatcaa gctgattttc gtcttgatca gcaattacat cccataaatc
    17101 ttccggctta ctatcctcac tatcatcttc aatttcatta accgtgtttt cgacgtggtt
    17161 tggattatat gctacgtcat ttgttgaaat atcttctact ggtgttccat tgagaattcg
    17221 cagaaccgtg aatccaacta aaaacaaggg aagtcctgca aaaatcaccg caaaaacgac
    17281 agcttgttgt agcggttttg gtaaaaatgc ggtacgttgt ttgataaatt taaccggctt
    17341 agcgagctgc tttccgaaat cgataaattt catagtatct ttcctaagat aaaaggttat
    17401 tgtttttggc gactactata aattacttta tctttattgt cttttacaga ataagataaa
//
LOCUS       DANLXF010000030        16755 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.030, whole genome shotgun sequence.
ACCESSION   DANLXF010000030 DANLXF010000000
VERSION     DANLXF010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16755
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.030"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..9438)
                     /locus_tag="PXD66_000603"
     CDS             complement(<1..9438)
                     /locus_tag="PXD66_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HDL6318933.1"
                     /translation="MNKHCFRTIFSKTLQRIVVVSELAKLAGKSESESMTSGAILQKI
                     CKIRPLAFSLFCALGFVTLSDNAMAETLIIQADSTAPKNQQPIILQTANGLPQINIQT
                     PNDKGLSHNKYSQFDVAEKGAILNNSRTNTATQQAGMVQGNPYLARGEAKVILNEVNS
                     SKPSVMKGYVEVAGKKAEVIIANPSGLHCDGCGIINADRATLTTGKPQINQGNLEGFV
                     VEKGKVTVAGKGLDNSRVDYTDILAREAKINAGVWSKKETKVITGKNTVKRSNSDNNL
                     QIIHTNQPLAGEEKPTVAIDVGELGGMYAGKIHLIGTEQGVGVRNAGHIGASADTLTI
                     DSQGRIVNSGTFNAQRQVQLNAQHGIDNQGKIENKQGNIQLNSKADIQNSGSIVARGG
                     NIQKKAETKIQQSGETVAKGNITYTARQIHADKNSLIAAGVDVTDTAQGEVRKLEAQS
                     VAGKTIQLTAPEKTTVQGKNIASGKINVAAAEIDANQSQNNAHTVEYSAKSGDIQANS
                     AQFSANQIKLTTPKWLSTEGSQLTAEVIEAQQANLNARGSVWKQTGEQDFRLKAESIH
                     TDSARFSVGGHFEIDGNRLTNNQGLLSSGKRLGLNLNDAVDSVDGKLVAKGELSIRAP
                     LLNNQQGLILSGEKQAITVNTLKNQSGVITSQTEQTLDITSELQNQGGRISASGVAIN
                     AQSLDNREQGEISSVKELALTVSQQVDNRHGVIQSVGNLTLNSTLLDNQGGVLKSATD
                     ITLNVPTVLNNRISENGSLIEAGGSLTMNSFEVENQGTLARQPQPIQGIIASQFVLNS
                     TKQLNNEKGGIYIGSSARFTLSDSLNNRNGEILSWGDLAIFGGKSLTMTNTEGKVSAN
                     NGLEITAKALSGDGDVQAGDIRLNLQENFDITKDINANRSMIIQTEGDILNRKKLAAN
                     DRLQLTANQITNAQNARISSADTRLTATSTVYNEGLINSRSDNDNAQTVIKAQEIHNI
                     GTGNIYGDKVALGAERILNQDKDGTSATIAARQSLGLAAKVIENNTLVYEANKKSGSL
                     IYSGGEIAIGRQLNEQDTVIGNAGQLHNRSSIIEAEGRIQLNADKVANVNSHYRSELE
                     TTDRQTVNEHFILPRDKHNRGGSDVYDNGHLPTINVNQLRKGYYRKTWSYWQKYDEAN
                     LPVITDISQLNATTVLAKPYAQSCANDESVDCTLLPAGNYKKDNPVWTWFNLTPPAED
                     APEMTPEIIQAMNEEDLANQHLSEAEQEKLQKEQGQGIAPVVPARPIEPVRGSTETEA
                     QYQKRLQTYQAELKAYEAAEAAYQRYVLLKPLIDWEAKYGANIQALDEAIAKHNKANL
                     SQEYPHFWNLWIKERMVKENITKASLPAQILAGGDLTYQSDEFINDKSWVIAGKALNP
                     IGRSRIANLDDADAVRQDWELGRRQFSFTEYHGGTRRRHSRYDENDGPLARLKEVHKD
                     MNIFVELENTSPTAYTDYVDAKAANAVKPEGNQVERGNLPDAPNPPTMAIERIPSPNA
                     AAFEVRSMTVDTRLPNQGLYRINPQADSHVLIETDPDFTDKKRWLSSDYMFNALRHDH
                     NNVHKRLGDGFYEQRLIREQINRLTGRQFLGDYTDFDSQYKALMDAGITFAQKFNLRL
                     GITLSPEQVASLTSDIVWFETQTVRLPSGETVSVLVPKVYALARKGDIDGKGSLISAD
                     ALTINSKEIVNQGTIAGRRLAQFNAERLENSGTLSGGVLAAKVSGDLENIGGKIEADK
                     AILLDIAGNFNHTSTTRTTQIDLDGFKRAETNLDRKALLHVKDENGLLQVQAGNITSA
                     GADIINDGQGQTYLSAKNQLNLTALQVGFDEKMGGGNHYRNEALNDVVISRVQGKGDV
                     VVSGKNLYSEGADLDAKARLALLAENDMVLGSATRSRDYEEYHKTKSGGALAKKTKTT
                     YDNISETEHKGTELTGEDILVKAGHDIQGESLLAVSRSGDIDIIGGNNVTLSSATNQL
                     SEQHIRETKRSGFLNGGEIGFTIGSQKTRQENSLEGAIQSTARNTLGSEGGNIRIQAG
                     NKASVSNVDVLISNEKTAVISGKNGVRVESGKDVINSTDNYEFSQSGLSIALSTPVTD
                     AVQSARQSVQKAKATQNEKLKGVYAVKAAEDAVIAAQNAQKVAETLGNLGNALTENHA
                     AAENPAVKISVSLGTQKQQRESRSQSVTHSKSTLNGGNIALISGAGKVALEGVDTRVK
                     DTLLLDGKLGIESKGVADTYQNNTKNKNHSASVGVFIGLDRDSFGIGFEAGVSGGKGK
                     ENIETETWQNNQLQAGKIVTNSANGKLMLDATNVKANHWEGEVQDFEARSRQDISKYK
                     SEYTEGSVHGTLAVGTTGVNGHVAYNGAKLNTAQVENQTAIDIGKSGMDVKVKKNAHF
                     EGAVMTSQAEKENNRFQAGTLTTGDIENHSELKTRSVAISGGTSGVNPMSALSLLGNK
                     NESERSTTKAAIGENIAITLTEDLNAETTLNHLNRDTQNANQKVTKYDISEVKETQEL
                     VKGIGEIAEKAYQIYTHSEREKVTEAKLELGKAQAQKASKEEIGKLEANLDRLQKEFD
                     KNYGTGSKTKRAVEAVTAALQGLAAKDVGQAAVGLASPYLNAEIKKYTEGDTQANLIA
                     HALLGAVEAAATGNNVLAGAAAGAGSEAAADFIVKTLYKGKSTQDLTEAEKQNVALLS
                     QLASGLASGLIGDSTQSAAVGADIGKRAVENNYLSNWQEQKRSQELKSCHGDARCENQ
                     VRLYWGLVDIGQDVSFVSGAVAGVPENLAESVEGLISMFSSPIETIVAMKELIAREDT
                     LSYIANMTKQEYLAKLDKMQVYYEQAGAVGSFNAGKEFGNMMTDVVTALTGAGALVKT
                     GVGTTAKIVTKSGKELAIPKTRSSASLGTCSFHGDMEVKTDKGYRQISSIKVGDKVLA
                     KNERTGITTYQKVQAHYSNPYDYTVYVEVADSKGKYHTIVSNKIHPFFTQVLSGRVPI
                     SSEGHSYNGEIAKAQWVDAQHLQKGYRLLAESGEWQTVTKVKIKQERLKAYNMTVEKD
                     HTYFIRGTNADNEGVWVHNDCWHALPNDAKQVKNIDGYKAYQFTDPKDGKVVTVVQKD
                     LNRFETPNHQAGVDPHFNRLSQQIDAETGRYLSTDNPVDGLYNRAYLRSETLQKIYAN
                     YEKLPNGNYRNKLTRQEIEQPIDIGHMY"
     gene            complement(9490..11286)
                     /locus_tag="PXD66_000604"
                     /pseudo
     CDS             complement(9490..11286)
                     /locus_tag="PXD66_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250636.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
     gene            11420..12505
                     /gene="queA"
                     /locus_tag="PXD66_000605"
     CDS             11420..12505
                     /gene="queA"
                     /locus_tag="PXD66_000605"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDL6318934.1"
                     /translation="MLVSDFHFNLPDELIARYPTAERSASRLLHLNGETGEFADQQFV
                     DLYDHINEGDLLIFNNTRVIPARLYGRKPSGGKVEALVERVLDEHRCLAHVKASKAPK
                     EGAELIFGEDKLGEGKGFTATMVARHDTLFELVFNEEQPLFELLQQAGHMPLPPYIDR
                     PDEDADQERYQTVYSKVLGAVAAPTAGLHFDNAMLEKLKAKGVDTAFVTLHVGAGTFQ
                     PVRVETIEDHKMHAEYAEVSQEVVDKILATKAKGKRVIAIGTTSVRSVESAAQAAEKD
                     GKLIAPFFSDTAIFLYPGKTFKVIDALVTNFHLPESTLIMLVSAFAGYRNTMNAYAHA
                     VETKYRFFSYGDAMFINKNPNALSDVP"
     gene            complement(12532..13070)
                     /locus_tag="PXD66_000606"
                     /pseudo
     CDS             complement(12532..13070)
                     /locus_tag="PXD66_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015701733.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13083..15365)
                     /locus_tag="PXD66_000607"
     CDS             complement(13083..15365)
                     /locus_tag="PXD66_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HDL6318935.1"
                     /translation="MTLDRFCIALILFHLPILWLPKAFLFVGIVIGTLVLILSVYLRS
                     KLTAVLGLLVCIGYIQIIQVAKNAEKTTASKTPVQFEISKILKQTDYQTAIATLNTGE
                     KIYLNWQSKQPLLLNQFYQAELALRPISARSNIGNFDRQRWYFANHINMTATVRKVET
                     LAKTDYSLRTNWLNKVKAETDALPTQGFLLALAFGERAWLKTEHWGQFQQTATAHLIA
                     ISGLHIGLAMLFGVYFAKGVQWLCLKSELTALQAVGFSLYFARIVSFITAFGYSYLAG
                     FAVPTARALLAIAFVLLCQVLRRHYTPWQYWGRIVALLILIDPLSLLSDSFWLSILAV
                     ASLILWYQYFPLSRFLNDEYRKKITKFNRLWLSLLHLQIGIWLIFSPVQLYFFEGISA
                     FALLANLIIVPLYSFLLVPLILFTLLTDNLFSTWLLADYLAQFSLWLLDPFSNAWFAL
                     SHWQQWQLLSLNLLVLVLLYCKLYRLSYKKWLSAVAVSLLFNLSFYLPKLFNQTQTEW
                     IMFDVGQGLAMAFIYQQNKAVIYDTGSSWQANDGSRNSMAKMEILPYLKRNGIEVEAI
                     FLSHDDNDHSGGVVELLQAYPKARLISSSKVTYHNINPEPCIAGTSWQFGEWQLLAIY
                     PSHIAERAKNQDSCVILAKNDRLKILLTGDSGVEQERQFAHQLGKIDFLQVGHHGSKS
                     STGETLLSITQPAFAVISAGRWNPWKLPNKQIIERLNRRHINVLNTVETGMIRVKFYP
                     SSIEIEQARGAFSPWYKQFY"
     gene            complement(15507..16721)
                     /gene="sstT"
                     /locus_tag="PXD66_000608"
     CDS             complement(15507..16721)
                     /gene="sstT"
                     /locus_tag="PXD66_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDL6318936.1"
                     /translation="MSQSLSSKILGGNLVLRIAIGLVLGVLLAFINKEWATSVGVLGQ
                     FFVKSLRAIAPILVFVLVLAAIANKEVGSDSKLKPILVLYVLGTFFAALTAVVLSFMF
                     PTTLELTASPDGLTPPQGIGEILKTVIFNLVDNPLVALSNANFIGILAWSIGLGIAFR
                     HGSASSKAFLNDLANAVSFVVKVVIAFAPIGVFGLVAETVAINGIDAFEDYARLLVVL
                     VGAMLFVALVLNPLLVYWKIRRNPYPLTFTCLRESGVTAFFTRSSAANIPVNMNLAKR
                     LGVRDEIASVAIPLGASINMAGAAITVTVLTLAAAYTQGIQPDFATALLLSVVASICA
                     CGASGVAGGSLLLIPLACSLFNIPNDIAAQVIGVGFVIGVIQDSVETALNSSTDVLFT
                     AAVSYAEDQKSA"
BASE COUNT         4301 a         3956 c         3362 g         5136 t
ORIGIN      
        1 atacatatga cctatatcaa taggctgttc aatttcttga cgggttaatt tattgcgata
       61 attaccattt ggcaattttt cataattagc ataaattttc tgtaacgttt ccgagcgtaa
      121 ataagctcgg ttatatagcc catctaccgg attatctgtc gataaatagc gtcccgtttc
      181 ggcatcaatt tgctgagaca aacggttaaa atgtggatct accccggctt gatggtttgg
      241 cgtttcaaaa cgattgagat ctttttgaac tactgtcacc actttcccgt ctttcgggtc
      301 tgtgaactga taagctttat agccatcaat attttttacc tgtttggcat cattcggtaa
      361 agcgtgccaa caatcattat gcacccatac cccttcatta tcggcatttg tacctctaat
      421 aaagtaagta tgatctttct ctaccgtcat attgtaggct tttaatcttt cctgtttgat
      481 ttttacctta gtgacagtct gccactcacc actttcagca agtaagcgat agcctttttg
      541 caaatgttga gcatcaaccc actgagcttt tgctatctct ccgttgtaag aatgcccctc
      601 cgaagaaatc ggcactctgc ccgataatac ttgcgtaaag aacgggtgga ttttgtttga
      661 cacaatggtg tggtattttc ctttgctgtc tgcgacttct acatacaccg tataatcgta
      721 tggattactg taatgagctt ggactttctg ataggtggtt ataccggtgc gttcattttt
      781 cgccagcacc ttatcgccca ctttaatgct ggaaatctgt cgataacctt tatcagtttt
      841 gacttccata tcaccgtgga atgagcaagt gcctaaacta gcgctcgatc tggtcttagg
      901 aatcgcaagt tctttacctg atttggtgac tattttcgcc gttgtcccta cacctgtttt
      961 aacaagagca ccagcaccgg ttaatgctgt caccacatca gtcatcatat tgccaaactc
     1021 ttttccggca ttaaaagaac ctacagcccc tgcctgttca tagtaaacct gcattttatc
     1081 gagtttagct aaatactcct gtttagtcat attagcaata taggagagcg tatcttctct
     1141 tgcgatcaat tctttcatcg ccacaatagt ttctattggg cttgagaaca tggagatcaa
     1201 tccttcaacg ctctcagcga gattctcagg aacaccagca acggcgcctg atacaaaact
     1261 aacatcttgc ccaatatcaa ctaatcccca atataatcga acttggtttt cacagcgggc
     1321 atcaccatgg caggatttta attcttgtga gcgtttctgc tcctgccagt tcgagaggta
     1381 gttattctcc actgcccttt tcccaatatc cgccccaacc gctgccgatt gtgtactatc
     1441 ccctatcaaa cctgaagcca gtccgcttgc aagctggctt agcaacgcca cattctgttt
     1501 ctcggcttcg gttaagtctt gcgtggattt gcccttgtag agggttttaa ctataaagtc
     1561 cgctgccgct tcgctccctg cgcctgcagc agcccctgcc agtacattgt tccctgtcgc
     1621 agccgcttcc accgcaccca gtaaggcgtg ggcaatcagg ttggcttggg tatccccttc
     1681 ggtgtacttt ttgatttctg catttaagta tggtgaggct aaacccactg ccgcttgtcc
     1741 cacgtctttc gccgctaaac cttgcaaggc tgccgtcacc gcttccaccg ctcgtttggt
     1801 tttactgccc gtgccgtagt ttttatcaaa ctctttttgc aagcggtcta aattggcttc
     1861 taatttacca atctcttcct tgctcgcttt ttgagcttgt gctttgccca attccagctt
     1921 cgcttccgtc actttttcac gctcactatg cgtgtaaatc tgataagcct tttctgcaat
     1981 ctcgcctatg cctttcacca gctcttgtgt ttctttcacc tcactgatat cgtatttcgt
     2041 cactttttga ttggcgtttt gcgtatctcg gttgagatga ttaagcgtag tttccgcatt
     2101 taggtcttcg gtgagggtga tagcgatatt ttctccaatc gctgcctttg ttgtgcttcg
     2161 ctcgctttcg tttttattgc ccagtaagct cagtgccgac atcgggttca ccccacttgt
     2221 ccctccgcta atcgcgacac ttcgggtttt caattcactg tggttttcaa tatcgccggt
     2281 ggtcagcgtg ccggcttgga agcggttgtt ttctttctcc gcttggctag tcattaccgc
     2341 tccctcaaaa tgggcgttct ttttcacttt aacgtccatt ccactcttgc ctatgtcgat
     2401 tgcagtttgg ttttccacct gagcagtgtt caacttcgct ccattatatg ccacatgacc
     2461 atttactcct gttgtcccta ccgccaatgt gccatgtacg ctgccttctg tgtattcgct
     2521 tttatatttg cttatatctt gtcgacttct tgcctcaaaa tcttgtacct caccttccca
     2581 atgatttgct ttgacattgg ttgcatccag catgagttta ccatttgcag aatttgtaac
     2641 aattttaccc gcttgcagtt ggttgttctg ccacgtttca gtttcgatat tctccttgcc
     2701 tttacctcca ctcactcctg cctcaaaacc aataccaaaa ctatctctat ctaaaccgat
     2761 aaatacgccc acactcgcac tgtgattctt attcttagtg ttgttttgat aagtatccgc
     2821 aacacctttg gattcaatac ccagcttacc gtcaagcagc agggtgtctt tcactcgggt
     2881 atccacccct tccagcgcaa ctttgcccgc tcccgaaatc aaggcaatat tgccgccgtt
     2941 cagggtgctt ttgctgtggg tcacgctttg actacggctt tctctttgct gtttttgcgt
     3001 gccgagactg actgaaatct tcaccgccgg attttctgcc gcagcatggt tttctgtgag
     3061 tgcattgcca agattgccca gcgtttctgc cactttctga gcattttgtg ctgcaatcac
     3121 ggcatcctcc gccgctttta ccgcatatac ccctttgagt ttctcgtttt gtgttgcctt
     3181 cgctttttgc accgattgcc gtgccgattg caccgcatcc gttaccggtg tactcaacgc
     3241 aatgcttaag cccgactggc taaattcata attatcggtg ctgttaataa catctttccc
     3301 tgattcaacc cgtaccccgt ttttgcctga aatcacggcg gttttttcgt tggaaatcag
     3361 cacatcgaca ttgctcacgc tcgctttatt gccggcttga atccgaatat tgcccccttc
     3421 actgcccaat gtattgcgtg ccgtgctttg gattgccccc tctaagctgt tctcttggcg
     3481 tgttttttgg ctaccgatag taaagccgat ttcaccaccg ttcaagaaac cgctgcgttt
     3541 tgtctcacga atatgctgtt ctgaaagttg gtttgttgcc gaactgagcg ttacattatt
     3601 accgccgata atatcaatat cgccactgcg tgagacggca agaagacttt caccttgaat
     3661 atcgtgaccg gctttcacta aaatgtcttc accggttagt tcagtgcctt tgtgttcggt
     3721 ttcagaaata ttgtcataag tcgtttttgt tttcttcgct agcgcaccac cactttttgt
     3781 tttgtgatac tcttcataat cgcgagaacg tgttgcgcta cccaacacca tatcattttc
     3841 agccagtaac gctaaacgcg ctttggcatc caaatccgct ccttcactgt aaaggttttt
     3901 ccctgaaacc accacatcgc ctttgccttg cacccgtgaa atgaccacat cattcaaggc
     3961 ttcattacga tagtgattgc caccgcccat tttttcatca aaaccaacct gtaaagcggt
     4021 caaattcaac tgattttttg cagataagta ggtctgcccc tgtccgtcat tgatgatgtc
     4081 tgcgcctgct gaggtgatgt tgcctgcctg tacttgcagc agtccatttt catctttaac
     4141 gtgcagtagg gctttgcggt caaggttggt ttcagcgcgt ttgaaaccgt ctaggtcaat
     4201 ctgtgtggtt cgagtggtgg aggtatggtt aaaattgccc gcaatatcca gcaaaatcgc
     4261 tttatccgct tcaatcttgc cgccgatatt ctctagatca ccgctcactt tggcagcaag
     4321 cacgccacca ctgagtgtgc cactattctc taaccgttcg gcattaaact gtgctaaacg
     4381 gcggcctgct atcgtgcctt ggtttacaat ctctttactg ttgatggtaa gcgcatcggc
     4441 tgaaatcagg ctgcctttgc cgtcaatatc acctttgcgt gcaagggcat acactttcgg
     4501 cactaatacc gataccgttt caccgcttgg caagcgaacc gtttgggttt caaaccaaac
     4561 aatatcgctg gtcaagcttg ccacttgctc cggcgataag gtaatgccta accgaagatt
     4621 gaatttctgc gcaaaggtga tgccggcatc catcagggct ttatattggc tatcaaaatc
     4681 cgtatagtcg cccaaaaatt gtctgccggt taagcggttg atttgctctc gaattaagcg
     4741 ctgttcataa aaaccatcgc ccaaacgttt atgcacattg ttatggtcgt ggcgtagggc
     4801 attgaacata taatcactgc ttaaccagcg ttttttatcg gtgaaatccg gatcggtttc
     4861 gatcagaaca tggctatccg cttgagggtt aatgcgataa agcccttggt ttggcaaacg
     4921 ggtatcgacc gtcattgagc gaacctcaaa tgcggctgca ttcgggcttg gtatgcgttc
     4981 aatcgccatt gttggcggat taggggcatc gggtagatta ccccgttcaa cctgattgcc
     5041 ttccggcttc acggcatttg ctgctttagc gtccacataa tctgtataag cggtcggtga
     5101 agtattttcc agctcgacaa aaatattcat atccttatgg acttctttta aacgagcgag
     5161 aggaccatca ttttcatcgt agcgggagtg gcgacgtctt gtgcctccgt ggtactcagt
     5221 aaaagagaat tggcgccttc ccagttccca atcttggcga acagcatctg catcatcaag
     5281 gttggcaatt ctactgcgac caatcgggtt taacgctttg cctgcaataa cccaactttt
     5341 atcgttgata aattcatcgc tttgataagt gagatcgcca cctgccaaga tctgtgccgg
     5401 taagctggct ttggtgatat tctcttttac catacgctct ttaatccaca aattccagaa
     5461 atgcgggtat tcttggctta aattcgcttt attgtgcttt gcaatcgctt catctaatgc
     5521 ttgaatattg gcaccatatt ttgcttccca atcaattaac ggttttaata gcacataacg
     5581 ctgataagcc gcttctgccg cttcataggc tttcagttcc gcttgatatg tttgtagtcg
     5641 tttttggtat tgggcttccg tttccgtaga gcctctaaca ggttcaatcg gtcttgccgg
     5701 aaccaccggt gcaatgcctt gcccttgttc cttctgtaat ttttcctgct ctgcctcact
     5761 taaatgctga ttcgctaaat cttcttcatt cattgcttga ataatttcag gcgtcatttc
     5821 cggtgcatct tcagctggtg gtgttaaatt aaaccacgtc catactgggt tatctttttt
     5881 ataattacct gcaggtaata gagtacaatc tacactttca tcattcgcac agctttgagc
     5941 atagggtttt gccaatacgg tcgtggcatt cagctggcta atatcggtaa ttaccggcaa
     6001 gtttgcttca tcatactttt gccaatagct ccacgttttg cgatagtagc ctttgcggag
     6061 ttgattcaca ttaattgtcg gcaaatgccc gttatcatat acatcacttc cacctcgatt
     6121 atgcttatct cttggcaaaa taaaatgttc gttcacagtt tggcgatcag tggtctccaa
     6181 ttccgaacga taatgactat ttacattcgc taccttatcc gcattcaact gaatacgccc
     6241 ttcagcttca ataatactgc tgcggttatg tagctgccct gcgttgccaa taacggtatc
     6301 ttgctcattt aattgacgtc caatcgctat ctctcctccg ctatatatta atgaaccgct
     6361 tttcttattc gcttcataaa ctagggtatt attttcaatt actttagccg ctaaaccgag
     6421 agattgacga gcagcaatag tggcagaggt cccgtcttta tcttgattta gtatcctctc
     6481 cgcacctaac gccaccttat cgccataaat attccctgtg ccgatattgt ggatttcttg
     6541 agctttaatc acagtttgag cgttatcgtt gtccgagcgg ctattgatta agccttcgtt
     6601 atagacggtt gaagttgcag tcaaacgggt atctgccgag cttattcggg cattttgtgc
     6661 attggttatt tggtttgccg tgagctgcaa gcggtcattt gcggctaatt ttttgcgatt
     6721 gagaatatcg ccctcagtct gaataatcat cgacctattc gcattgatat ctttggtaat
     6781 atcaaagttc tcttgcaaat tcaggcgaat atcgcctgct tgtacatcac catcaccgga
     6841 taacgctttg gcagtgatct ccaaaccatt atttgcagaa acttttccct cggtattcgt
     6901 cattgtgagt gattttccgc cgaaaatagc cagatcgccc caacttaaaa tttccccatt
     6961 acggttattt aagctgtcgc tgagggtaaa tcgtgctgat gagccgatat aaataccgcc
     7021 tttttcatta ttgagctgtt ttgtgctgtt cagtacaaac tgcgatgcaa taatcccctg
     7081 tattggctga ggttggcgtg ccaaggtacc ttgattttcc acctcgaaac tgttcatcgt
     7141 aagtgagcca cctgcttcaa taagtgagcc attttccgag atccgattat ttaagacagt
     7201 cggtacattg agcgtgatat ctgtggccga tttcagtacc ccaccttggt tatccaaaag
     7261 cgtgctattt agggtgagat ttcccacact ttgaataaca ccgtgccgat tatctacttg
     7321 ttgggaaacc gtgagcgcta actcttttac tgaactaatt tctccctgct cacggttatc
     7381 taaggattga gcgttaatcg caacaccgct ggcggaaatt cgcccgcctt gattttgcag
     7441 ttcgctagtt atatcaaggg tttgctccgt ttgagaggtg atcacccccg attgattttt
     7501 taacgtattg accgtaatcg cctgtttttc gccactcaaa atcagacctt gctgattatt
     7561 cagcaatgga gcacgaatag ataactcgcc ttttgcaacc aatttgccat cgactgaatc
     7621 cacagcatca tttaaattta gccctaaacg cttgccacta cttaatagcc cttggttatt
     7681 tgttaaacga ttaccgtcaa tctcaaaatg tccacccact gaaaagcgag ccgagtcagt
     7741 atggatactc tctgctttta ggcgaaaatc ctgctcacca gtctgcttcc aaaccgagcc
     7801 acgagcattt aaattcgctt gttgggcctc aatcacctct gcggttagct ggcttccttc
     7861 agtggaaagc cattttgggg tggtgagctt gatctgattt gcactaaact gcgcactatt
     7921 cgcctgaata tcgcccgatt ttgcactgta ttcaacggta tgggcattat tttggctctg
     7981 attggcatca atctcagctg ccgccacgtt aattttcccc gaagcgatat tttttccctg
     8041 cacagtggtt ttttctgggg cagtgagttg aatagttttc cctgccacag attgagcttc
     8101 cagcttccgt acttcacctt gtgcagtgtc ggttacgtcc accccagcgg caatcagtga
     8161 gtttttgtcg gcgtgaattt ggcgggcggt gtaggtgata ttgccttttg cgaccgtttc
     8221 gccactttgc tggattttag tttcggcttt tttctgaata ttgccacccc tcgccacgat
     8281 ggaaccgcta ttttgaatat cggctttcga gttaagctga atattgccct gtttattttc
     8341 aattttgcct tggttgtcaa tcccgtgttg ggcattgagt tgcacttgtc tttgagcgtt
     8401 gaacgtgccg ctgtttacaa tccgcccttg actgtcaatg gtgagcgtat cggcacttgc
     8461 accgatatgc ccagcgttac gcacgcccac gccctgttcc gtaccaatca ggtggatttt
     8521 gccggcatac attccgccca gctcacccac atcaatcgca actgtgggct tttcttcccc
     8581 tgcaagcggt tgatttgtat gaataatttg caaattgttg tccgaatttg accgcttgac
     8641 ggtgtttttg ccggtaatga ctttagtctc ttttttcgac cagacaccgg cgttgatttt
     8701 cgcttctcgg gcgaggatat cggtgtaatc cactcggctg ttgtccagcc ctttgccagc
     8761 aaccgttacc ttgccttttt ccactacaaa accttctaaa ttgccttgat ttatctgtgg
     8821 ttttccggtg gtcagcgtgg ctcggtcggc gttgataata ccgcagccgt cacagtgcaa
     8881 gccgctcggg ttggcgataa tcacttccgc ttttttgcca gccacttcca cataaccttt
     8941 cattaccgaa ggcttactgg aattgacttc atttaaaatc accttcgctt cgccacgggc
     9001 aagatacggg ttgccttgca ccataccggc ttgttgggtg gcggtgttgg tgcggctgtt
     9061 gttgagaatt gcccccttct ccgctacgtc aaattggctg tatttattgt gggaaagccc
     9121 tttatcgttc ggggtttgaa tattgatttg tggcagcccg ttggcggttt gcaaaataat
     9181 cggttgctga tttttcgggg cagtgctatc ggcttgaata atcaaagttt ccgccatagc
     9241 gttgtctgat aaggtaacga atcccaacgc acagaacaag ctgaacgcaa gcggtcgaat
     9301 tttgcaaatt ttttgtaaaa ttgcaccgct tgtcatactt tcactttctg atttacctgc
     9361 taattttgct aattcagata ccaccacaat acgttgtaag gtcttgctga aaatagtgcg
     9421 aaaacaatgc ttattcataa taattcctta atagcacaat atacttaata caatatgccc
     9481 aatcattcat taaaaattgt aaccaatatt aaaaccggca acggtgtctg aggttttaaa
     9541 cccttccggt tttcgcatcg gtctaccggc aaacacgtca taactaaatc ctttaactcc
     9601 accccgaaac ccaatggctg ttcccattaa atgatgtccg agctgatttt ccgttgccca
     9661 accggacact cgaccaccat ctaccgccca ataaaaagcg tggttcggat taaattgcca
     9721 gctcaactca ttacgccagt tccagccccg ctcaccggaa agagtcagtt caccgtcaaa
     9781 tccacgtatg gtgtaacgcc cgccaatcga aaaacggtct tgcggaacca gaggggtttt
     9841 attccattgg gcatttaggc tactttgcca ctgccatggc tgcttgcccc aaaaaaacgg
     9901 tttcgtcagg cttgcacttg ccgtgatgat tttagggcga gaagtccctt cgccataatc
     9961 ttcctcggga gcagataatg cccctcgtgc tgccgtacct cgtttgaaat tagccgaaag
    10021 ctcaagcgta gcggtcggga aatattcttt atgcgaaaaa cccgcttccc aacctgccat
    10081 tcggcgtttt tgtacttcaa tttctgagcc gtcaatatag ttttgcgact ggcgtggcca
    10141 aagtgaaccg gacaagctcg ttttacgttt ggcatcacga taaagcaaat aagaaaaggt
    10201 taatttatcg gtatcacttt caccggcgta gagatagctc ttgtcaaagg caccaaatac
    10261 ttcttgatga taacgatgat gagtgtgtga aaatgctaac tgccaatagc caaacggcac
    10321 ggaatagtaa agcgtgagat ttttactggc acgtctgccg ttatcatcac ctttttgttt
    10381 catcgaatgg gtaaaagagg tatagaacag gtcatttgcc gaaaaaatat tatctataga
    10441 cagagtggcg gaggtttgta atttgcctgt ggatttcgag ccggcatcgt ccagcccaag
    10501 atttaagcga aatggaaacg cttgcgaata gctgattttt aaatcactgt ctcccacctc
    10561 cgctttttcg cttgagctgg gtaaaatctc aatatttgcc tccgccgtcg gcacccgttt
    10621 tagattttcc agagactgct caatatctcg cacattcaga atatcgcctt cggcaaaagt
    10681 aaaaccggtt aaggcgtgca gcttggtaaa acgcggtact ttgccgctat ctaccactaa
    10741 agtattgcgt attttgcccg gaatcacggt taaaactaat tttccgccac gtaaatcttg
    10801 ctccgcagta accactcgtg tagtgacata acctttgcca ataatttcat tttggatttg
    10861 cttcatcaac acagctaacc cttcaccacc aaagcagtga ggtaatgtca aatgaaatga
    10921 ttttactgct ttttcaaacg cccattggaa ttggctggta gtcagagaat tgtcaggaga
    10981 atagtcaatg agagtgattt gatgaatggg gtaacaaggc aattcgtgat tggaaagagt
    11041 aagtgtatca ttgatttgtg tctcgagccg tacatcagct ttcggctgga atttttctgc
    11101 ttgattttgt tggtattgct gttagcgatg ttgctgggca gcatcaattt gttgttgagt
    11161 tgcatcaatg cgtgcttgtt gtacaaggct tgaagaattt gcttcagcaa caaaagaagc
    11221 acttaacaat accgtgctta aaatcagatt ttgtggggtt ttcataagat caaataagta
    11281 agacattcaa cgtttttagt ctataaaaat ttatgttgtt ttgcaaactc cgttttatgg
    11341 agctttatac atatctgacg actttggttt tttatgctat tatctccgcc atttttatac
    11401 tatagagaat agaataaccg tgttagtttc agattttcat ttcaacttac ccgatgagct
    11461 gattgctcgc tacccgactg ccgaacgctc tgccagccgt ttattacact taaatggcga
    11521 aacaggcgaa tttgccgatc aacaatttgt ggatttatac gatcatatca acgaaggcga
    11581 tctattgatc ttcaataaca ctcgagtgat cccggctcgc ctttacggtc gaaaacctag
    11641 tggcggcaaa gtagaggcat tagtggaacg ggtgctagat gaacaccgct gcttggctca
    11701 cgttaaagcc tcaaaagcac cgaaagaagg ggctgaattg atctttgggg aagataagct
    11761 aggtgaaggc aaaggcttta cggcgacaat ggtagctcgc cacgatactc tgtttgaatt
    11821 ggtgtttaac gaagagcaac cgttattcga attgctacag caagctggtc atatgcctct
    11881 tccaccttat atcgaccgcc ccgatgaaga tgctgaccaa gagcgttatc aaaccgttta
    11941 tagcaaagtg ttaggtgcag ttgccgcccc taccgccggt ttgcattttg acaatgcaat
    12001 gcttgaaaaa ctaaaagcaa aaggggtgga tactgccttc gtaacattgc acgttggtgc
    12061 cggcactttc cagccggtac gggtagaaac cattgaagat cataaaatgc acgccgaata
    12121 tgccgaagtg agccaagaag tagtcgataa aattctcgca acgaaagcca aaggaaaaag
    12181 agtgattgca attggtacga catcagtacg ttcggttgaa agtgccgccc aagcggctga
    12241 aaaagatggg aaattgattg ctccgttctt ttctgacacc gctattttcc tttacccggg
    12301 aaaaaccttt aaagtcattg atgcgttagt caccaatttc catttaccgg aatccacgct
    12361 aattatgtta gtctcagcat ttgccggcta tagaaatacg atgaatgcct acgcccacgc
    12421 agtagaaact aaataccgtt ttttcagcta tggcgatgcg atgtttatca ataaaaaccc
    12481 aaatgcgttg agtgatgttc cttaatttat gagggttata agggaataac attatgaaca
    12541 aaaacaatct tccaaccatt ttaagggatt gttttcaata tattcaataa tttggaagta
    12601 cgatgtttca ttacgaataa tgtgttcata ataattcctt tgccaaattt cagaaatatc
    12661 ggtattttga cgaacccatt tagttacgcc aattttaaaa ccacgcacaa tagagccgat
    12721 tgttcgtgat gttccccgta agggcgaaac atttttcgcc cttatacatg gggcaaatga
    12781 tgatttgccc ctacagtttc gacgatttca ataatgccat gtaaatgatt aggcataatc
    12841 acaaagctat gcaagcttac atttggaaaa tgatcgggta tatcttccca acattgacgt
    12901 acaatattcc ctatcttatt aagcctcatt tcatcatcaa caatctcacc gagaaaatgg
    12961 tgtttatttt tacaacaaat agtaatgaaa tagaatcctt gtgatcggta atcataatgc
    13021 tttagacgaa ttgaacgcct cctatgaatc tccggctgat atttctgcat ttttcttatt
    13081 ccttaataaa actgcttata ccaaggggaa aatgcccccc ttgcctgctc aatttcaata
    13141 gaacttgggt aaaattttac tcttatcatt cccgtttcta cggtatttaa tacattaata
    13201 tgtcttctgt ttaatctctc aataatctgt ttattcggta atttccatgg gttccatctt
    13261 ccagcagaaa tcactgcaaa tgctggctgg gtaatcgata ataaagtttc gccagtgctg
    13321 cttttgctac cgtgatgtcc aacctgtaga aaatcaattt tgccgagctg atgggcaaat
    13381 tggcgttctt gttccacgcc agaatcccct gtcagtaata tcttcaagcg gtcgtttttg
    13441 gctaaaataa cgcaagagtc ttggttttta gctcgttctg caatgtgtga cgggtaaatt
    13501 gccaataatt gccattcgcc aaattgccac gatgttccgg caatacaagg ctcgggattt
    13561 atattgtgat acgtcacctt gcttgaacta attaaccttg cttttgggta tgcttgcaat
    13621 aactccacta ccccacctga atgatcatta tcatcgtgac ttagaaaaat cgcctctacc
    13681 tcaatgccat ttcgctttaa atacggcaag atttccattt ttgccattga attgcgacta
    13741 ccatcatttg cttgccaact ggagccggta tcgtaaatta ccgctttatt ttgttgataa
    13801 atgaacgcca ttgccagccc ttgcccaaca tcaaacataa tccattctgt ttgggtttga
    13861 ttaaagagct tagggagata aaaactcaga ttgaacaata gggataccgc tactgcactt
    13921 aaccactttt tataagatag ccgatataat ttgcaataca gcaatactaa aaccaataaa
    13981 ttcagtgaaa gtaactgcca ctgttgccaa tggcttaatg caaaccacgc attagaaaat
    14041 ggatctaaca gccataggct aaattgagca agatagtccg ccaacagcca cgttgaaaac
    14101 agattgtcgg tcaagagcgt aaataaaata agcggtacaa gcaaaaagct atacaacggc
    14161 acaataatca ggttggcaag cagtgcaaaa gcagagatgc cctcaaaaaa gtagagttga
    14221 accggtgaga aaatcagcca aatgccgatt tgtaagtgca aaagcgataa ccacaagcgg
    14281 ttaaattttg tgattttttt acgatattca tcgtttaaaa atcggctaag aggaaaatat
    14341 tgataccata aaattaaact tgctaccgct aaaattgaga gccaaaaact atcagaaagc
    14401 aggctcaatg ggtcaattaa aatcagtaac gccactattc tcccccaata ttgccaaggg
    14461 gtatagtgtc gccgcagaac ttggcaaagc aagacaaacg caatcgccag caacgctctg
    14521 gcggtcggca cggcaaaacc ggctaaatag ctgtaaccaa atgcggtgat gaagctaact
    14581 attcttgcaa aataaagtga aaatccgacc gcttgtaatg cggtaagctc gcttttcaag
    14641 caaagccatt gcacgccttt ggcaaagtaa acaccaaaca acattgccag cccgatatgc
    14701 aagcctgaaa tggcaattag atgggcagta gcggtttgtt ggaattgccc ccagtgttct
    14761 gttttcagcc acgccctttc accaaaagcc agagccaata aaaagccttg cgtaggcaaa
    14821 gcgtccgttt cagctttcac tttattcagc caatttgttc gcaagctata atcagttttg
    14881 gctaatgttt ccaccttgcg aaccgttgct gtcatattga tatgattggc aaaataccaa
    14941 cgctgtcggt caaaattacc gatattgcta cgagctgaaa taggacgaag agccagttcg
    15001 gcttgataga attgattaag taacaacggc tgtttcgatt gccagttcaa gtagatcttc
    15061 tcgcctgtat ttagtgtcgc aatcgctgtt tgataatcgg tttgttttaa gattttactg
    15121 atttcaaatt gaacaggcgt tttactagca gtcgtttttt ccgcattttt tgcaacttga
    15181 atgatctgaa tatagccaat acaaaccaat aagccgagta cagccgttaa ttttgagcgt
    15241 agataaacac tcaatatcag cactaaggtt cctattacta tacccacaaa tagaaacgct
    15301 ttaggcaacc ataaaatagg cagatgaaat agaatcaggg caatacaaaa acggtctaaa
    15361 gtcataattc ggcttacttt ttaaagctga gtatgccgtt tgtgcttaat attttcagaa
    15421 taaattttca gattttcgac acagttcgca aaaatacttg aaaaaataaa ggcagacttt
    15481 ttagcctgcc ttataaagaa gagatattat gctgattttt gatcttcagc gtaacttact
    15541 gctgcagtga ataatacgtc tgttgatgaa tttaatgcgg tttcaaccga gtcttgaatc
    15601 acaccaatca caaaacccac cccaattact tgagcagcaa tatcgtttgg aatattaaat
    15661 aaactacagg ctaatggaat caataataat gaaccgcctg ccacacctga tgcgccacaa
    15721 gcacagattg atgcaactac gcttaatagt agtgctgttg cgaaatcagg ttgaatacct
    15781 tgtgtataag ctgccgctag ggttaatacg gttacagtaa tcgctgcacc tgccatatta
    15841 atacttgcac ctaatggaat agctacagaa gcgatctcat cacgaacgcc caaacgcttg
    15901 gctaaattca tattcaccgg aatgttggct gctgaactgc gggtaaagaa tgccgttaca
    15961 ccactctctc gtaaacaggt aaatgtaagt gggtatgggt tgcggcgaat tttccaatac
    16021 actaataatg gatttagtac taatgccaca aataacattg cgccaactaa gactactaat
    16081 aaacgtgcgt aatcttcaaa cgcatcaata ccattgatgg caacagtttc agctactaaa
    16141 ccaaataccc caataggcgc aaatgcaatc acaactttca caacaaacga aactgcgtta
    16201 gctaaatcat ttaagaatgc ttttgaactt gccgaaccat ggcggaatgc aatccctaaa
    16261 ccgatagacc acgcaagaat accgataaag tttgcattac ttaatgcgac taatggatta
    16321 tcaactaagt taaaaataac ggtttttaga atttcaccaa tgccttgtgg cggggttaaa
    16381 ccgtctgggc ttgcagtaag ctctaatgta gttgggaaca taaagcttaa aacaacagcc
    16441 gttaatgccg caaaaaatgt acctaataca taaagcacca gaatcggctt taatttgcta
    16501 tctgaaccaa cttctttatt tgcaatcgca gccaaaacta atacaaatac taaaataggc
    16561 gcaatggcac gtagtgattt cacaaaaaat tgacctaata caccaacgct ggtagcccat
    16621 tctttattaa taaatgccag caatacccct agcactaaac caatggcgat acgtaatact
    16681 aaattaccgc caagaatttt tgatgataat gattgactca taacccttca cttctgtaag
    16741 acaacaaaaa ataag
//
LOCUS       DANLXF010000031        16749 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.031, whole genome shotgun sequence.
ACCESSION   DANLXF010000031 DANLXF010000000
VERSION     DANLXF010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16749
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.031"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..695
                     /locus_tag="PXD66_000609"
     CDS             <1..695
                     /locus_tag="PXD66_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDL6318937.1"
                     /translation="NSWKYAFKLITPIFMGYFAAGVAYGMLATNAGMPAWFTIVMSFT
                     VVSGTAQYAAIPFFVSGVGAVSVFLSTLLMSLRFSFYTLNMMEHKPISKAKSLVAIAG
                     LTDEGFAVLSSLPKEQKQALFFKIAVLCVAYWTFSTIVGVLLGDSVANYIPHLDFALP
                     CLFAILAYEQYKNQKQWKPIVIALIGFLLARQITETSVLLVAILVAIVIVALLPQSSS
                     IKLEKGVENEHK"
     gene            706..1008
                     /locus_tag="PXD66_000610"
     CDS             706..1008
                     /locus_tag="PXD66_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDL6318938.1"
                     /translation="MFAALVLGSQICRFLPEFLPKKVLSSPILQKLNKMLPLVIMLLL
                     LLTSLNFPKNGEGFNLILAQILALIGVLGSYVWLKNTFLSVALGIFGVNVFLSLLG"
     gene            complement(1129..2430)
                     /locus_tag="PXD66_000611"
     CDS             complement(1129..2430)
                     /locus_tag="PXD66_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL6318939.1"
                     /translation="MSLFKFEERGSNARQEIIAGLTTFLAMVYSVIVVPGMLSQAGFP
                     AESVFIATCLVSGLGSILIGLWANAPMAIGCAISLTAFTAFSLVLGQQVSIPVALGAI
                     FLMGVVFTLISATGIRTWILRNLPSSIAHGAGIGIGLFLLIIAATNVKLVVNSGMDIP
                     VKLGDFTSFPVLVSLIGLAAIIGLEKLKVKGSILWVIIAITIVGLIFDPNVKYTGFFK
                     MPSFGENSQFLNLDIMGALNTAILPVVFALVMTAIFDATGTIRAVAGQAGLLDKDGQI
                     INGDKALTSDSFGSILSGLFGTAPAAVYIESAAGTAVGGKTGLTAVVVGIGFLFMLFF
                     QPLAFLVPSYATAPALMYVGLLMLSNVSKLDFSDFVGAMSGLVCAVFIVLTANIVTGI
                     MLGFATLVIGRLISGEANKLNLGTVIIAIILVAFYAFDLAI"
     gene            complement(2563..3537)
                     /gene="secF"
                     /locus_tag="PXD66_000612"
     CDS             complement(2563..3537)
                     /gene="secF"
                     /locus_tag="PXD66_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HDL6318940.1"
                     /translation="MAIENVQQDVAEIKLPYKLIPFMKYRYVGFIFSLIVTALCIFTI
                     ATKGFNWGLDFTGGTVIETQFSQPADLAKLRTELENSGYGNALVQTTGSQKDLMIRLA
                     ASAGDMSVGNKVMEMIHQKLDPNATIKSIEFVGPNVGEELTQGAIYATLATLAMLLIY
                     VGTRFEWRLAVGGIVALFHDVLVTIGIFSFLQIEIDLTFVAAILSVVGYSLNDSIVVF
                     DRVRENFSKVRRSSSIEIIDISLSQTLSRTLMTSVTTLFVVLALFYLGGPTLHSFSLA
                     LLIGIGFGTYSSIYIAIGVALQLGLKREHMVQPKVEKEGADQDAFIDY"
     gene            complement(3547..5388)
                     /gene="secD"
                     /locus_tag="PXD66_000613"
     CDS             complement(3547..5388)
                     /gene="secD"
                     /locus_tag="PXD66_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HDL6318941.1"
                     /translation="MLNRFPLWKNLMVIFVIAIGALYALPNLYGDDPSVQISGTRGQQ
                     ATPETLSQVQSTLSAMNIQPKSAVLENGSILVRLESSEQQLPAKEKISEALGNNYSVA
                     LNLAPATPQWLSDIGGSPMKRGLDLLGGVRFLMEVDMNTALAKQQDTLQDSLRSDFRK
                     EKLQYQAVKKGENFATEVEFADNDTAEKAVRLVRLSHPTLDILVNDNKVTLTPSAQAL
                     SESRTMAIEQNLSILRKRVEELGVSEPTIQRQGADRIVVELPGVQDTARAKELLGATA
                     TLEFRLVNTEANVASAAKGIVPADSGLQNTRDGEPVVLRRKPSLGGEHIIDATSGTDE
                     RGLPQVSIRLDTEGGEMMGEITKMAIQKPMATLYTEFKDSGRKDANGKVILEKHQEVI
                     NVATIQTRLGNQFQITGINSPAEAQNLAVLLRSGALIAPIVIVEERTIGASLGADNVK
                     QGMEASILGLGLTILFCLAFYKVFGIFASLALIVNMILTIGLMSLIGATLTMPGIAGI
                     VLAVGMSVDANVLIYERIKEEIRNGRPIQQAIHEGYNGAFSSIFDSNLTTILTSLVLY
                     GVGTGPVKGFAVTLALGVIISMFTAITGTRMLVNWVYGGKRVKKLWI"
     gene            complement(5428..5721)
                     /gene="yajC"
                     /locus_tag="PXD66_000614"
     CDS             complement(5428..5721)
                     /gene="yajC"
                     /locus_tag="PXD66_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDL6318942.1"
                     /translation="MQQGGGMEMIFILIIFGAIFYFMIYRPQAKRQKQQRDLLASLTK
                     GDEVLTSGGLIGKITKVTADNDNIVIALNDTTEVTIKRDFVVAVLPKGSLKSL"
     gene            5922..6479
                     /locus_tag="PXD66_000615"
     CDS             5922..6479
                     /locus_tag="PXD66_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HDL6318943.1"
                     /translation="MKKLFAQAVIFGAFFASSAFAHNVQLNQALPAVSVAKDGELAVA
                     GGKVSYKNWHSSSLAGKVRVIHHFAGRSSVKEKNEDLMTAIKNAGFDRSKYQTVTIVN
                     ADDAIVGTGVFVKNSVENGKLDNPHSQVVLDQQGSVKKAWGLKEKESFIAVLDKSGKV
                     QFTSEGKLSAKQIQEVIGLVKTLTQ"
     gene            complement(6520..7488)
                     /locus_tag="PXD66_000616"
     CDS             complement(6520..7488)
                     /locus_tag="PXD66_000616"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HDL6318944.1"
                     /translation="MKLFTLENAFLRITLSDVGASWLSCIVKMPNEEREVLVTTSAKN
                     WQKQTAFFGATIGRYANRIANAEYRLNGKTYQLAKNNGEHNLHGGELGADKIVWQLES
                     QSEQAVKFSYFFADGEEGFGGEVKAFVEYRLTENELQISFDAVANQDTPLCLTNHAYF
                     NLQGSETILDHELIINADGYLPVDASGIPNAPLKKVESTSFDFRNAKKIGQDLLADDD
                     QKAVKGYDHSFHLAKNSENPTACLAVEDLKMELRTSMPALQLYTGNWLKGQPNLTKGE
                     YEDYAGVALEPEFFPNSLNQPELLQFGGITKAGEEYHHTISYRFII"
     gene            complement(7614..8777)
                     /gene="galK"
                     /locus_tag="PXD66_000617"
     CDS             complement(7614..8777)
                     /gene="galK"
                     /locus_tag="PXD66_000617"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDL6318945.1"
                     /translation="MKPQEIALQQFEQHFGYKAERTVFAPGRVNIIGEHTDYNDGFVM
                     PCAINYGTAVSFAKRSDNKWRVFAIDLNQQDEFDLSQAIEPSEQKWANYVRGVVKFIQ
                     AKHPEFKQGADLAITSDVPMSSGLSSSAALEVSIGKTAQLLGDLPLSLPQIALIGQEA
                     ENKFVGCNCGNMDQLTSALGQQDHLVMIDCRSLEITPTPVPQGYSIAIINSNVKHDLV
                     TGEYNSRREQCEFAAHFFGKAKLRDVTAEQFIERASELSEENELAYKRAKHIISENQR
                     VLEAVEALKANDMVKLGELMAQSHDSMRDDFEITCPEVDCLAEIAQQAIGKNGGARMT
                     GGGFGGCVVCLVPNDKVEDLRQAVANNYEKQTGIKETFHVCTASDGVRECPAR"
     gene            complement(8787..9833)
                     /gene="galT"
                     /locus_tag="PXD66_000618"
     CDS             complement(8787..9833)
                     /gene="galT"
                     /locus_tag="PXD66_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HDL6318946.1"
                     /translation="MSEFVLNDHPHRRFNPLKNQWILVSPHRAKRPWQGQQEEIVQDE
                     KPSYDPSCYLCPGNKRITGEQNPNYTKPFVFKNDFSALLPDTPAPIPNDDPLFQISHT
                     QGESRVICFSPDHSKTLPQLSIEEITDVVTVWQEQANELKEHYQWVQIFENKGSMMGC
                     SNPHPHGQIWASNFLPNEIAIEDECQAKYFAQYGSPLLLDYAQRELDKQERIVAETED
                     WIAVVPYWAAWPFETLLLPKAKQFKSITDLNEAEKADLALALKKLTTRYDNLFNISFP
                     YSMGFHFAPFNGKENAHWQLHAHFYPPLLRSATVRKFMVGYEMMAESQRDLTPEQAAE
                     RLNAVSDTKHYKDK"
     gene            10046..10897
                     /locus_tag="PXD66_000619"
     CDS             10046..10897
                     /locus_tag="PXD66_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF817 domain-containing protein"
                     /protein_id="HDL6318947.1"
                     /translation="MERLNKFDAWLMNHKPNARLSGASRLLTEFWFFGLKEARACLFA
                     GLFFIAMFSVPKTGLWGLPRYDILLIFAIVVQIAMVKLGLETWDELKAISLFHLVGFV
                     LEVFKTSSNIQSWAYPDFAYTKLFNVPLFTGFMYAAVGSYIIQAWRLFDLKIKSHLPY
                     WLATLTALMIYLNFFTHHYIGDYRWYIAAFALGLYARTFVFFTPYDITRKMPLLLSFF
                     LIGFFIWLAENIGTFAGIWRYPNQFGAWSMVHMSKWSAWALLVMMTFTIIVHLKHIKQ
                     TVRVSSD"
     gene            complement(10983..12068)
                     /locus_tag="PXD66_000620"
     CDS             complement(10983..12068)
                     /locus_tag="PXD66_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL6318948.1"
                     /translation="MEYRYYITTALVIAVLQLFLFNFAKTVSWLFNLSSKNRKTVGIL
                     TFLVANGLVILHLTRTFALFRIMAFILVLLLFAFFISFFTACIYKIFNGNTTVNRWLK
                     IAYPFAFFGLLGLGYYNAYTPTVVHYSVKLDKPLEKPLRIGMASDLHLGKLFGTKQLD
                     QLAEIFNQQKVDLILLPGDIMDDNTEVYVADKMQPHLAKLKAPLGVYATLGNHDFFGA
                     QETIAKEITDAGITVLWDQTAEINGKFTLIGRNDDLVKNRPSAEQLLQNVNTKLPVIL
                     LDHRPTEIEQHAKLPIDIQVSGHAHKGQVFPASIITKLIYRLHYGYEKIGFGHYFVTS
                     GYGFWGVPMRLGSQSEVMIIDVEGENP"
     gene            complement(12090..13070)
                     /locus_tag="PXD66_000621"
     CDS             complement(12090..13070)
                     /locus_tag="PXD66_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDL6318949.1"
                     /translation="MLFDSIQFSIGVTLPTILLMLFGIFPRRRKFIDDTFCNMASKVV
                     FNFTLPPMIFLNMLKSPLDFSSQINLISTGVGGSILIYLWAEWWAARHIENRRYRCIF
                     TQGTFRTNAAILGIALILNAYGDKALAAASVYISCLVITFNVLAVITLTSSLSEKKVN
                     FGRLILSILRNPLILAVIAGLTFNYFELSHQIPTPLESAGKLMASLTLPLALICTGAS
                     INFKQLKQFNQGVESTINKIVLFSASIRLIFAPIFLLLLGKFVFQLPPMELGIVFVAA
                     SAPVASATYAMTRNYGGDGEAAANLIGITTLGSMFSASIGLFLLRQIGWV"
     gene            complement(13173..14271)
                     /gene="prfB"
                     /locus_tag="PXD66_000622"
     CDS             complement(join(13173..14195,14197..14271))
                     /gene="prfB"
                     /locus_tag="PXD66_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014550889.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDL6318950.1"
                     /translation="MFELNPIKNQLTDLSERTAVIRGYLDFEHKVERLEEVNAELEQP
                     DVWNKPEKAQALGKERAMLEQIVNTIKALEQGIEDVEGLIELAVEAEDQDTFNEAQVE
                     ADELEKKLADLEFKRMFSGPHDSADCYLDLQAGSGGTEAQDWTEMLLRMYLRWAESKG
                     FKAELMEASDGDVAGIKSATVKISGDYAYGWFRTETGIHRLVRKSPFDANNGRHTSFS
                     AAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNKTESAVRITHIPSGIVVQCQN
                     GRSQHQNKDQAMKQLKAKLYELEMSKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DHRTGVEHRTTQQVLDGDLDKFVEASLKAGL"
     gene            14527..15435
                     /locus_tag="PXD66_000623"
     CDS             14527..15435
                     /locus_tag="PXD66_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDL6318951.1"
                     /translation="MAKRPSDIDATLLQLEILRRIPKLPAKIDAKSLHQQLLDAGFER
                     DIRTVQRSLKMLCEHFDIECDERSKPFGYSWKIRSEGLALPILNEQQSLLLKLAEQQL
                     KYLLPANLMASMKPFFEQADRMVSGAKDKPEYQWLNKVCSTPTSLPLIPAKIKEEIFN
                     SVSQALFQNKLLKIEYQNQHGKKHQATIMPLAISQQGASIYLVCRYEGFDDNRLLALH
                     RIRKAEISTFSFERPKDFNLQAYQDAGHLGFGNGEKASLTFSISRDAGFHLTETPLAK
                     DQKILEESETHYRIQAIVADNDMLTW"
     gene            15736..16377
                     /locus_tag="PXD66_000624"
     CDS             15736..16377
                     /locus_tag="PXD66_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HDL6318952.1"
                     /translation="MPFQLTFCQQAGNQHQKNQDALFNGKAVYQWQLKNAESYLLDND
                     KVIFGVADGVSSHPRNHLASHYLMALLSQCQSLDSQWLRQAHGDFCEKLAKDYFGASC
                     TFVACELHHNGRGRMVNVGDSRAYKITANGEWQQLSFDHTILAEMKQKGLVKEGSNYA
                     SMYNGLTDCIVADFDADDFRIHIAEFQLEKGESILLCSDGLHEQLAPEAIQAI"
     gene            16544..>16749
                     /locus_tag="PXD66_000625"
     CDS             16544..>16749
                     /locus_tag="PXD66_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HDL6318953.1"
                     /translation="MKNDFHYAAELIRNADGILITAGAGMSVDSGLPDFRSVGGLWNS
                     YPPLKKFNLQFMQIATPLAYQERP"
BASE COUNT         4779 a         3518 c         3566 g         4886 t
ORIGIN      
        1 aaaatagttg gaaatatgct tttaaattaa tcacgccgat ttttatgggc tattttgcgg
       61 caggggttgc ttacggaatg cttgccacca atgccggaat gcctgcgtgg tttacgattg
      121 taatgagttt taccgtggta tcggggacgg cacaatatgc agcgattcca ttttttgtct
      181 ccggtgttgg ggcggtgtcg gtctttctca gtacgctttt aatgagtcta cgttttagtt
      241 tttacacctt aaatatgatg gagcataagc cgatatccaa agcgaaaagt ctggtcgcta
      301 ttgcaggttt aactgatgaa ggctttgcgg tgttatcttc attaccgaaa gagcaaaagc
      361 aagcattatt ctttaaaatc gctgtgctat gtgtggctta ttggacattt tccacgattg
      421 tcggtgtgtt gttaggcgat tcagtggcga actatattcc gcatttggat tttgctctgc
      481 cttgtttgtt tgcaatttta gcctatgagc aatacaaaaa ccaaaaacaa tggaaaccga
      541 ttgtgattgc gttaatcgga tttttacttg ctcgtcagat taccgaaacc agcgtgttat
      601 tggtggcaat tttagtagcg attgtcattg tggctttatt acctcaatct tcttcaatca
      661 aactagagaa aggagtagaa aatgagcaca aatgatactt tgataatgtt tgcagcatta
      721 gtgctcggct cgcaaatttg tcgttttttg ccggaatttt taccgaaaaa ggtgttatct
      781 tcgcccattt tgcaaaaatt aaacaaaatg ttaccgcttg tgattatgct tttattgctt
      841 ttaacgagcc taaactttcc taaaaacggg gaaggcttta acttaatatt ggcacaaatt
      901 ctggctttaa taggcgtatt aggtagctat gtttggctta aaaatacctt tttaagcgta
      961 gcgttgggga ttttcggtgt gaatgtattt ttatccctgt taggataaat accaaaggtc
     1021 attttatagg agcgaaacac cttcgctcaa tcattaacag atgtggggcg aaaaattttt
     1081 cgccccttat ttatcacaag cggtcttttt ctttcaattt tttgcaaatt aaatcgctaa
     1141 atcaaacgca tagaaggcaa ctaaaataat cgcaataatt actgtaccta ggtttaattt
     1201 attggcttcg cctgaaatta agcgaccaat cacaagggtg gcgaaaccta gcataatgcc
     1261 tgtcacaatg ttggcggtta atacgatgaa caccgcacag actaaacctg acattgcacc
     1321 tacaaaatcg ctgaaatcta atttagagac attacttagc attaataagc ccacatacat
     1381 taatgccggt gcggtggcat agcttggtac taagaatgca agaggttgga agaacagcat
     1441 aaataagaaa ccgataccaa ccactacagc ggttaaacct gttttaccgc caacggctgt
     1501 acctgctgcc gattcaatat acactgccgc tggtgctgta ccaaataagc ccgataaaat
     1561 tgatccgaat gagtcagaag ttagggcttt atcgccattg ataatttgtc cgtctttatc
     1621 taataaaccg gcttgccctg caaccgcacg gatagtacct gttgcatcaa aaatagcggt
     1681 cattactaac gcaaacacta ccggtaaaat ggcggtattt aatgctccca taatatcaag
     1741 atttaggaat tgcgaatttt caccaaaact cggcattttg aaaaagcctg tgtattttac
     1801 gttagggtca aaaattaaac ctacgatggt aatagcaata atgacccata aaatactgcc
     1861 ttttactttc agcttttcta acccgataat ggcggctaga ccgattaatg acactaaaac
     1921 cggaaatgaa gtgaagtccc ctaatttaac cggaatatcc ataccgctat tgaccactaa
     1981 tttaacgtta gtggcggcaa taatcagtaa gaataaacca ataccaattc cagcaccgtg
     2041 ggcaatgctg cttggcaaat tacgtaaaat ccaagtacga ataccggtag cagaaatcaa
     2101 ggtaaatact acgcccatta agaaaatcgc cccgagtgca acaggaatag aaacttgttg
     2161 ccctaatact aagctaaagg cagtaaaagc agtaagtgaa atcgcacaac cgattgccat
     2221 tggggcattt gcccataaac cgattaagat agagccgaga cctgaaacta aacaggtggc
     2281 aataaatacc gattcggcag gaaagcctgc ttggcttagc attcccggca caacgatgac
     2341 ggaataaacc atggctaaaa aggtagttaa accggcgatg atttcttgac gagcgtttga
     2401 accacgctcc tcaaatttaa ataacgacat aagaacctca aaagaagaaa atgaaagggg
     2461 aaattttagg atgcggaatt ttacagaaat ttaaaataat tagcaaacgt ttgcgtaaaa
     2521 agtgaaagaa aagcaggaat aaatcctgct tttgtttgat ggttagtaat cgatgaaagc
     2581 gtcttgatct gccccttcct tttcaacttt aggctgaacc atatgttcac gttttagccc
     2641 aagttgtagt gccacaccaa ttgcaatgta gatggatgaa taagtaccga aaccgatacc
     2701 gatcaataat gcaagtgaga aactatgaag cgttggacca cctagataga ataaggctag
     2761 taccacaaag agggtggtta ctgatgtcat taaagtacgc gaaagtgttt ggctcaagga
     2821 aatatcgata atttcaattg agctggagcg acgaacttta gaaaagtttt cacgtacacg
     2881 gtcaaacacc acaattgagt catttaacga gtaaccgaca acagataaaa tcgctgctac
     2941 aaaggtcaag tcaatttcaa tctgtaagaa agagaagatt ccgatcgtca ctaatacatc
     3001 gtgaaataaa gccacaatac cgcctaccgc taaacgccac tcaaaacgtg ttcccacgta
     3061 aataagtagc atagcaagtg ttgccaaagt ggcataaata gccccttggg ttagctcttc
     3121 ccccacgttt gggccaacga attcaatgct cttgatagtc gcattcggat ctaatttttg
     3181 atggatcatc tccatcactt tattcccaac agacatatca ccggcactgg cggcaagtcg
     3241 gatcattaaa tctttttgac tgcctgtggt ttgcactaag gcattgccgt agccgctatt
     3301 ttcaagctcc gtacgcagtt ttgctaaatc agcaggttgt gaaaattgag tttcgattac
     3361 tgtcccgccg gtaaaatcta acccccagtt aaagcctttt gtcgcaatag taaaaatgca
     3421 taaagcagtt acaatcagcg aaaaaatgaa tccgacatag cggtatttca taaaggggat
     3481 taatttataa ggcagtttaa tttctgctac atcttgttgt acattttcaa tagccatttt
     3541 tccaccttaa atccaaagtt tcttcacgcg tttgccaccg taaacccagt ttacaagcat
     3601 tcgagtacct gtaattgcgg taaacataga aataattacc cctaatgcaa gcgttaccgc
     3661 aaagcctttt accggacctg tacctacacc ataaagcaca agagaagtca aaatagtggt
     3721 gaggttggag tcaaaaatac ttgagaatgc gccattataa ccttcgtgga ttgcttgttg
     3781 aattgggcga ccgttacgaa tctcttcttt gatccgctcg tagattagta cgttggcatc
     3841 aactgacata cccacagcaa gtacgatacc ggcaattccc ggcatagtaa gggttgcacc
     3901 gattaatgac attaaaccaa tcgttaagat catattcaca atcaaggcaa gagaggcaaa
     3961 tataccaaat actttataaa aggctagaca gaacaggatc gttaagccta agcctaaaat
     4021 actagcttcc ataccttgtt taacattatc tgccccaaga gaagcaccga tagtacgctc
     4081 ttccacaatc acgattggag caatcaacgc acctgaacgt aataataccg caaggttttg
     4141 agcttccgct ggtgaattaa tccctgtaat ctggaattga ttacctaaac gggtttgaat
     4201 ggttgcaacg ttaattacct cttggtgttt ttctaaaatg actttaccgt tagcatcttt
     4261 acgacctgaa tctttaaatt cggtataaag tgttgccatc ggtttttgga ttgccatttt
     4321 agtaatttca cccatcatct cgccgccttc ggtatcgagt cggatactca cttgcggcaa
     4381 gccacgctca tcagtgcctg aggttgcatc aataatatgt tcacccccta acgaaggttt
     4441 acggcgtaat acaactggct cgccatcacg ggtgttttgc agtcctgaat cagccggcac
     4501 aatgcctttc gccgcagagg ccacattggc ttcggtattg actaaccgga actcaagggt
     4561 tgcagttgcc cctaaaagct ctttggcacg agcggtatct tgcacacccg gtaattcaac
     4621 tacgatacga tctgcacctt gacgctgaat ggtcggctca gaaacgccca attcctcaac
     4681 acgtttacgc aaaatagaaa ggttttgttc gattgccatc gtgcgagatt cagaaagagc
     4741 ttgtgctgat ggcgttaagg tgactttatt atcatttacc aaaatatcaa gcgttgggtg
     4801 agagagacgc actaaacgca ctgctttttc ggcggtatcg ttatcggcaa attcaacctc
     4861 agttgcaaaa ttttcgcctt ttttgaccgc ttgatattgc aatttttctt tacggaaatc
     4921 actacgcagg ctgtcttgca aagtgtcttg ttgttttgca agcgccgtgt tcatatccac
     4981 ttccattaag aaacgaacac cgccaagcaa gtccaagcct cgcttcatcg ggctaccgcc
     5041 aatgtcagac aaccactgtg gcgttgctgg tgcaaggttt aaggcaacag agtagttatt
     5101 ccccaatgct tctgagattt tttctttggc aggcaattgc tgttcgctgc tttcgagacg
     5161 aaccaaaatc gagccatttt ctaacacggc agatttcggc tgaatattca ttgctgaaag
     5221 tgtggattgc acttgagaca gtgtctcagg tgtggcttgt tgcccacgag taccggaaat
     5281 ttgtaccgaa ggatcatcgc catataaatt tggaagggca tataaagcac caatggcgat
     5341 cacgaagatc accattaaat tcttccatag agggaaacgg tttaacacaa taactccccg
     5401 aatgttaaag ttggataaga gggttgatta aagtgatttt aatgaacctt ttggtaacac
     5461 cgcaacaacg aaatcacgtt tgatggtcac ttcagttgtg tcatttaatg caatcacgat
     5521 gttatcgtta tcagcggtca ctttggtaat tttaccgatt aaaccaccgc tggttaatac
     5581 ttcatcgcct tttgtcaagc ttgccagtaa atcacgttgt tgtttttggc gttttgcctg
     5641 aggacgataa atcataaaat agaaaattgc accgaaaata atcaggataa agatcatttc
     5701 cattccgcca ccttgttgca taaaaactcc ttaaatgttt gagtggtata aaaataagtg
     5761 ttataaaaaa cgcctgttaa aataccataa attcaatgaa ttaacgagta gaaaaattca
     5821 tcgaatgctt ttcttttaaa caaaaatttg agtagaatca gcacttttag tttaggatta
     5881 ggttcttggc gtgttcttgc gctgacacaa taggagaaaa aatgaaaaaa ttattcgctc
     5941 aagcggttat ttttggggct ttttttgcaa gttccgcttt tgctcacaat gtgcaattaa
     6001 atcaagcatt acctgctgtt tcggttgcaa aagacgggga attggcagtg gcaggtggaa
     6061 aggttagcta taaaaattgg cattcatcat ctctggcagg taaagtccgt gttattcatc
     6121 attttgccgg tagaagttcg gtgaaagaaa aaaatgaaga tttgatgaca gcaattaaaa
     6181 atgccggttt tgatcgctcg aaataccaaa cggtgacgat tgtaaatgct gatgatgcga
     6241 ttgtcggcac aggggtattt gtgaaaaata gtgtggaaaa cggcaaactt gacaatcctc
     6301 acagccaagt agtgttagac cagcaaggca gtgtgaaaaa agcgtggggc ttaaaagaga
     6361 aagagagctt tattgcagtg ctggataaat cgggtaaggt acaatttacc tctgagggca
     6421 aactctctgc aaaacagatt caagaagtga ttgggttggt gaaaacatta acgcaataac
     6481 cagtgttttt atgggcggat tttgtatccg cccatcattc tagataataa atcgataact
     6541 tatcgtgtga tgatactctt ctcctgcttt tgttatcccg ccaaattgga gtaattcagg
     6601 ctggtttagt gaatttggga aaaactcagg ctctaatgct acaccagcgt aatcttcata
     6661 ttcgccttta gtcaaattag gttgtccttt tagccagttg ccggtgtaaa gttgtaatgc
     6721 tggcatggag gtgcggagtt ccatttttaa gtcttccacg gctaaacaag cggtcggatt
     6781 ttcactattt tttgcaagat gaaaggagtg atcgtaacct tttaccgctt tttggtcgtc
     6841 atcagccaat aaatcttgcc caattttttt agcattgcgg aagtcaaaac tggtactctc
     6901 cacttttttc agtggagcat taggaatacc gctggcatca accggtaagt atccgtctgc
     6961 attaattatc agctcgtgat ctaaaatcgt ctctgaacct tgcaaattaa aataggcgtg
     7021 attagttaag caaagcggtg tatcttggtt agcaacggca tcaaagctga tttgaagctc
     7081 attctctgtt aagcgatact ccacaaaagc tttcacttcg ccgccaaatc cttcctcgcc
     7141 atctgcaaaa aaataactaa atttgaccgc ttgttcgctt tggctttcaa gttgccacac
     7201 tattttatct gccccaagct cgccaccgtg gagattatgt tcaccgttat ttttagcgag
     7261 ctggtaggtt ttgccgttta agcgatattc ggcattggca atgcggttag cgtaacgccc
     7321 aatggtcgct ccaaagaaag cggtctgttt ttgccaattt tttgcagacg tggtgactaa
     7381 tacttcccgt tcctcgttcg gcattttgac aatgcacgaa agccaagaag ccccaacatc
     7441 agaaagggta atgcgtaaaa acgcattttc aagggtgaat aatttcatcg gttgacctta
     7501 attacctagc acggcatagc cgtactaggt taagcataat tcagccctgc tagggctgag
     7561 gttatttatt ttagagtagc ggagctactt ccttatgctt aacccggtac gctttagcgt
     7621 gccgggcatt ctcgcacccc atcggaggca gtacaaacgt ggaaggtttc tttaatgccg
     7681 gtttgctttt cgtaattgtt agcaacggct tggcggagat cttccacttt gtcatttggc
     7741 actaggcaca cgacacaacc gccaaagccg ccgccagtca ttctggctcc gccgttttta
     7801 ccgatagctt gttgggcgat ttcggctaag caatccactt cagggcaggt gatttcaaaa
     7861 tcatcacgca ttgagtcgtg agattgagcc atcaactccc cgagtttgac catatcattc
     7921 gcttttaagg cctctactgc ttccagaact cgttgatttt cgctgatgat atgttttgct
     7981 cgtttatacg ccagctcatt ctcttcacta agttctgagg ctcgttcaat aaattgttcc
     8041 gctgtgacat cacgcaattt tgctttgccg aagaagtggg cggcaaattc gcactgttca
     8101 cggcgactgt tgtattcgcc ggtaaccaag tcgtgtttta cgttggagtt aataatcgca
     8161 atcgaatagc cttgtggcac aggggttggg gtgatttcta aactacggca gtcgatcatc
     8221 actaaatgat cttgttgccc gagagcggag gtgagctgat ccatattgcc gcagttgcag
     8281 ccgacaaatt tattctcagc ctcttgcccg attaatgcga tttgcggtaa agaaagcggt
     8341 aaatcgccga gtaattgagc ggttttacca atcgacactt ccaatgcggc ggaagaactt
     8401 aaacctgatg acatcggcac atcgctggta atggctaaat ctgctccttg tttgaattcg
     8461 gggtgtttgg cttgaataaa tttcaccacg ccacgcacat aattcgccca tttttgctcg
     8521 cttggctcaa tcgcctggct taaatcaaat tcatcttgtt ggttgagatc aatggcgaac
     8581 acccgccatt tgttgtcact acgtttggca aagctaaccg ctgtgccgta attgatggca
     8641 caaggcatta caaacccatc gttgtaatca gtatgttcgc cgataatatt cactctgccc
     8701 ggggcaaata ccgtgcgttc tgctttataa ccaaaatgtt gttcaaattg ttgcaaagcg
     8761 atttcttgtg gtttcatttc aattccttat ttatctttat agtgtttggt atcacttact
     8821 gcatttaaac gttcggcagc ttgttccggg gttaaatcac gttggctttc tgccatcatt
     8881 tcataaccga ccataaattt acgcactgtt gccgaacgca agagcggcgg atagaagtga
     8941 gcgtgtaatt gccagtgagc attttccttg ccgttaaacg gagcgaagtg aaagcccatt
     9001 gaataaggaa agctgatgtt aaacagattg tcgtaacggg tggtgagctt tttcaatgca
     9061 agggctaaat ccgctttttc cgcttcgttt aaatcggtaa tggatttgaa ctgcttggct
     9121 ttcggcaaca gcaaggtttc aaacggccaa gccgcccagt aaggcacaac cgcaatccaa
     9181 tcttcggttt ctgccacaat tcgctcttgt ttgtctaatt cacgttgtgc gtagtcaagc
     9241 aacagtggcg agccatattg agcaaaatat ttcgcttggc attcgtcttc aatcgcaatt
     9301 tcattaggta gaaaattact tgcccaaatt tgcccgtgtg ggtgagggtt agaacacccc
     9361 atcattgagc ctttgttctc aaaaatttgc acccactgat aatgctcttt cagctcattc
     9421 gcttgctctt gccaaactgt taccacatca gtaatttcct cgatggaaag ttgcggtaag
     9481 gttttactat gatcaggcga aaaacagatc acacggcttt cgccttgtgt atgagaaatc
     9541 tgaaacagcg gatcatcatt cggtattggt gccggtgtgt ctggtagcag ggcagagaag
     9601 tcgtttttaa acacaaacgg cttggtgtaa ttcgggtttt gttcgccggt aatccgctta
     9661 ttgccggggc agaggtaaca acttgggtcg tagctcggtt tttcatcttg cacgatttct
     9721 tcttgttgcc cttgccacgg gcgttttgcc cgatgcggag agactaaaat ccattgattt
     9781 tttaatggat taaaacgacg atgtgggtga tcgttgagga caaattcgct catataaaac
     9841 tcctcgaaat tagttatgta aagagtatag cgaaagtcag acaaaatgac tgtgacatag
     9901 ctcacaaaat aatgatgtca agcggtcaaa tttcagaaaa attttgcaag ttttcctcca
     9961 ttttgcgatt taaggtagct tacgtggaat tgaatgtggc ttttggtaga atagatgaat
    10021 atgcaagata ttaagacaaa aatagatgga aagactgaat aaatttgatg cttggttaat
    10081 gaatcataaa cctaatgcta ggttaagcgg agcgagtcgt ttgttgacag agttttggtt
    10141 ctttgggtta aaagaagcta gagcctgttt atttgcggga ttatttttta ttgcgatgtt
    10201 ttcggtacca aaaacgggat tatggggctt acctcgttat gatattttgc tgatttttgc
    10261 catagtggtg caaattgcaa tggtgaaact ggggctggaa acgtgggacg agctaaaagc
    10321 aataagccta tttcatttgg ttggttttgt gttagaagtc tttaaaacat cgagtaatat
    10381 tcaatcttgg gcatatccgg attttgccta tactaaattg ttcaatgtgc cattatttac
    10441 tggttttatg tatgcagcag ttggttctta cattatccaa gcgtggcggt tgtttgattt
    10501 gaaaattaaa agccacctgc cctattggtt agccacttta acggcactga tgatttacct
    10561 gaatttcttt acgcatcatt acattgggga ttaccgttgg tatattgccg cttttgcatt
    10621 agggctgtat gcacgtactt ttgtattttt caccccttat gacattacac gaaaaatgcc
    10681 actgctgctt tctttcttcc tgataggttt tttcatttgg ttggcggaaa atatcggtac
    10741 gtttgccgga atttggcgct acccgaatca gtttggggct tggtctatgg tgcatatgag
    10801 caagtggagt gcttgggcgt tattggtgat gatgactttt accatcattg tgcatttaaa
    10861 gcatatcaag caaacagtca gagtctcgag cgattaacaa gctgtgggtt ttggcgaaaa
    10921 ttttactcat cagttatttt cagtggaatt aggggcaaat aataatttgc ccctatatca
    10981 aattatggat tttccccttc cacatcaata atcatcactt ccgactgtga gcctaaccgc
    11041 atcggcacgc cccagaatcc gtagccggag gtgacaaaat aatgcccgaa gccgattttt
    11101 tcatagccgt aatgtaatcg atagatcagt ttggtaataa tacttgcagg gaacacttgc
    11161 cctttgtgag cgtgtcctga aacttgaata tcaatcggca atttagcatg ttgctcaatt
    11221 tcagtcgggc ggtggtcaag caaaattacc ggtagtttag tgttcacgtt ttgtaaaagc
    11281 tgttcggcag aaggtcgatt tttgactaaa tcatcattac gcccaataag ggtaaatttt
    11341 ccgttaattt cggccgtttg atcccataaa acagtaatac cggcgtcggt aatttctttt
    11401 gcaatcgttt cttgagcccc aaaaaagtcg tgattgccaa gggtagcata cacgccaaga
    11461 ggagctttta atttggctaa atgaggctgc attttatcgg caacataaac ttcggtattg
    11521 tcgtccataa tatcgcccgg caataaaatc agatcgactt tttgctggtt gaaaatctct
    11581 gccagttggt caagttgctt ggttccgaac agttttccta aatgcagatc gcttgccata
    11641 ccaatgcgaa gcggtttctc taatggttta tccaatttca ccgaataatg tactactgtt
    11701 ggggtataag cgttgtaata gccaagccca agtaagccaa agaatgcaaa tgggtaggcg
    11761 attttcagcc agcgattgac ggtagtattg ccgttgaata ttttgtaaat acaagcggtg
    11821 aaaaaactga taaaaaatgc aaaaagtaac aataccaata tgaatgccat tattctaaat
    11881 agcgcaaaag tacgagtaag atgcaaaatc actaatccgt ttgcgactaa gaaagtgaga
    11941 ataccaactg ttttacggtt tttacttgat aaattaaata accagctgac agtctttgca
    12001 aagttgaata aaaagagttg taacaccgca atcacaaggg cggtggtaat gtagtaacga
    12061 tattccatat ttttccttca ataaattggc taaacccagc cgatttgtct taataaaaat
    12121 aagccgatgc tggcactaaa cattgagccg agagtcgtaa tgccgatcag atttgcggct
    12181 gcctcgccgt ctccgccata gttgcgtgtc attgcgtagg tggctgaagc aacgggagca
    12241 gaagcagcca caaagacaat gcctagttcc atcggcggaa gttgaaacac aaatttcccg
    12301 agtagtagca agaaaattgg agcaaaaatc agccgaatcg aggcagaaaa cagcacaatt
    12361 ttattgatgg tgctttccac tccttgatta aattgtttca actgtttgaa attaatgctt
    12421 gctccggtgc agataagtgc taatggtagt gtcaggctcg ccattaattt accagcagac
    12481 tcaagcgggg ttggaatttg gtggcttaat tcaaaatagt taaaagttaa gcctgcaatg
    12541 acggctagaa tcagcggatt tctcaatatc gacaatatta atctgccaaa attcactttt
    12601 ttctcgctta acgatgaagt gagggtaatc actgcaagca cattaaaggt aatcaccaag
    12661 cacgaaatat aaactgaagc ggcagcgaga gctttatcgc cataagcatt gagaattaag
    12721 gcaatgccta aaatcgccgc attggtacgg aaagtgcctt gagtgaaaat acaacgataa
    12781 cggcggtttt caatatgtcg agccgcccac cattccgccc ataaatagat caaaatcgaa
    12841 ccgccgactc cggtcgagat caggttgatt tggctggaga aatctaacgg gctttttagc
    12901 atattcaaga aaatcatcgg tggcagggta aaattaaata ccacctttga tgccatattg
    12961 caaaaggtat cgtcaataaa ttttcgtctg cgtggaaaaa taccgaacag cattaacaaa
    13021 atggttggta aggtaacgcc aatgctaaat tggatagagt cgaaaagcat aatgtttgcc
    13081 ttaaatgatg gcacgagtgt cttttgcttg taccgtagat tatcaataaa gtaccagcgt
    13141 ggacgctggt actatcatca atggaaatga aattacaatc ctgcctttaa gcttgcttcg
    13201 acaaatttat ccaaatcacc gtccagcact tgttgggtgg tgcgatgttc cacaccggta
    13261 cggtggtctt taatccgtga atcgtctagc acataagagc ggatctggct gccccagccg
    13321 atgtcggatt tgttgtcttc catcgcctgt ttttccgcat ttttcttgct catttccagc
    13381 tcatatagct tcgctttaag ctgtttcatc gcctgatctt tattttgatg ttgtgaacga
    13441 ccgttctggc actgcaccac aatgccgctc ggtatatggg taattcgcac cgccgattcg
    13501 gttttattga cgtgctgacc acccgcccct gaggcacgat acacatcaat ccgcaaatcc
    13561 gcagggttga tttcaatatc aatatcgtca tcgacttccg gataaacgaa agccgccgag
    13621 aacgaggtat gacgaccgtt attggcatca aacgggcttt tacgcaccaa gcggtgaata
    13681 cccgtttcgg ttctgaacca gccgtaagca tagtcgccgg aaattttcac ggtggcggat
    13741 ttaatccctg ccacatcacc gtctgaggct tccattagtt cggctttaaa ccctttgctc
    13801 tctgcccaac gcaaatacat acgcagtaac atttctgtcc agtcctgagc ttccgttcca
    13861 cccgaaccgg cttgcaaatc caaatagcaa tcggcactgt cgtgtgggcc gctgaacatc
    13921 cgcttaaatt ctaaatcggc aagttttttc tctaattcgt ctgcttccac ttgggcttca
    13981 ttgaaggtat cctgatcttc tgcttccacc gccagctcaa tcaacccttc aacatcttca
    14041 atcccttgct ctaaggcttt aatcgtattg acgatctgct ccaacattgc ccgctcttta
    14101 ccaagcgctt gtgctttttc cggcttgttc catacatcgg gttgttccag ctcggcattg
    14161 acctcttcta agcgttcgac tttatgctca aagtcaaaga taccccctga tcaccgcggt
    14221 gcgctcgctt aaatcagtta gttggttttt aattgggttt aattcaaaca ttgtcgttcc
    14281 tgtttttgct tattttaaaa agatgaatta tagctgaagt taagcaagcg gtcgaatttt
    14341 gcaagaaatg gagcaatgat cgtttctagt aatgagattt ggttcataaa agttgcaact
    14401 gtttctgcta tttggctttg atagctaaaa atctttaaaa aaacaagcgg tcgttttacc
    14461 ccattttttg caaaacgacc gtagcttctt gctataatcg ctgaaaaaat gctgcaagga
    14521 gattttatgg caaagcgccc aagtgatatt gatgcaaccc tattgcaact ggaaatccta
    14581 cgccgtatcc ccaaattacc cgctaaaatt gatgctaaaa gtttgcacca acagctatta
    14641 gatgccggct ttgaacggga tattcgcact gttcagcgtt cgctcaaaat gttatgcgag
    14701 cattttgata ttgaatgcga tgagcggagc aaaccctttg gttatagctg gaaaatccgc
    14761 tcagaggggc tggcattgcc gattctcaac gaacaacaat cattactgct aaaacttgcc
    14821 gagcaacagc tgaaatatct gttacccgca aatttaatgg catcaatgaa accctttttt
    14881 gagcaggcag acagaatggt ttccggggcg aaagacaagc ccgaatacca atggctaaat
    14941 aaagtttgct ccacgccaac cagcctaccg ctgatccctg caaaaattaa agaagagatt
    15001 tttaattccg taagccaagc cttatttcaa aataagttgt taaagatcga atatcagaac
    15061 cagcacggca aaaaacacca agcgacaatt atgccgttgg cgatttcaca acaaggtgcg
    15121 agcatttatt tggtctgccg ttacgaaggg tttgacgaca accgcctgtt ggctcttcac
    15181 cgcattcgta aagcggagat ttctactttt agttttgaac gcccgaaaga ttttaatttg
    15241 caagcctacc aagatgccgg acacctcggt tttggcaatg gcgaaaaagc tagccttacc
    15301 ttttcgatta gcagagacgc cggtttccat ttaaccgaaa caccgcttgc caaagatcaa
    15361 aaaattctcg aggagagtga aactcactac cgtattcaag ccatagtcgc tgataatgat
    15421 atgctaacct ggtagctacg caaatttggc gaagatattt gggatatcga gaaagaagcg
    15481 ctaaaataag ctaaacaagc ggtcggtttt gtgggaattt ttgctaaata gtatcgaccg
    15541 ctcctcccta aacagacctt tatcataaat acaactcttt caatcagata ttcttactgt
    15601 ctattgatga acgattcgaa tttttctagt ggttgaaatt taaactaatt ttcttgcttt
    15661 ttggcatttt tcagactttt aattctctcc attagcctgt ttttttacgc tctttttcat
    15721 aaacaggaat aaccaatgcc ttttcaactt actttctgcc aacaagccgg caatcagcac
    15781 cagaaaaatc aagatgcact gtttaacggc aaagcggtgt accaatggca acttaaaaat
    15841 gccgagagtt atctgctgga taacgacaaa gtaatttttg gggttgcgga cggcgtttcc
    15901 agccaccctc gaaatcatct tgcctcacat tatttaatgg cgttactcag ccaatgccaa
    15961 tcccttgata gccaatggtt gcgacaggct cacggcgatt tttgcgaaaa attagcgaaa
    16021 gattattttg gtgcttcttg cacttttgtt gcttgtgaat tacaccataa cggcagaggg
    16081 cgtatggtta atgtgggcga cagtcgagcc tataaaatta cggcaaacgg ggagtggcaa
    16141 caactgagtt ttgatcacac catactggct gaaatgaaac agaaaggctt ggttaaggaa
    16201 gggagcaact atgcctcaat gtataacggc ttaaccgatt gtattgttgc cgattttgac
    16261 gccgatgatt tccgcattca tatagccgaa tttcaactgg aaaaaggcga gagcattttg
    16321 ctctgttctg acggtttgca cgaacaactc gccccagaag ctattcaggc gatttaggac
    16381 ggatacgaaa gcccgtctca tcgtctgcaa atttgcagaa aaatggtaaa aatgcaccgc
    16441 ttgtatgacg atttttctgc ggtgatttgt gaaattgtgt aattaaaacg acattatttg
    16501 tcgcattggt gcttaaaatc cgcataaaac agaggagaaa aaaatgaaaa acgatttcca
    16561 ttatgccgct gaattaatcc gcaatgcaga cggcatttta attaccgcag gggctggaat
    16621 gtcggtggac agtggcttgc ccgatttccg tagcgtggga gggttatgga attcctaccc
    16681 accgctcaaa aaatttaatt tgcaatttat gcagattgcc acaccgcttg cctatcagga
    16741 acgccctga
//
LOCUS       DANLXF010000032        16720 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.032, whole genome shotgun sequence.
ACCESSION   DANLXF010000032 DANLXF010000000
VERSION     DANLXF010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16720
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.032"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..412
                     /gene="nudB"
                     /locus_tag="PXD66_000626"
     CDS             <1..412
                     /gene="nudB"
                     /locus_tag="PXD66_000626"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HDL6318954.1"
                     /translation="VVIYAKNTQRVLMLQRKDDPDFWQSVTGSINIGETPLEAAEREV
                     SEETGLQTFEQKQPLVDCQKQIEFEIFPHFRYKYAPNITHCVEHWFLLPLDSEQEPIL
                     TEHLAYRWVSVEDAVRLTKSPNNAQAIQEYLTSK"
     gene            425..1165
                     /locus_tag="PXD66_000627"
     CDS             425..1165
                     /locus_tag="PXD66_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL6318955.1"
                     /translation="MAGHSKWANIKHRKAAQDAQRGKIFTKLIRELVTAAKLGGGDTS
                     SNPRLRAAVDKALSSNMTRDTINRAIERGVGGGDDTNMETKIYEGYGPGGTAVMVECL
                     SDNANRTISQVRPSFTKCGGNLGTEGSVGYLFSKKGLILVANADEDALTEAAIEAGAD
                     DIQPQEDGSFEIYTAWEDLGSVKDGIEAAGFKIENAEVTMIPSTTVELDAETAPKLLD
                     LINRLEDCDDVQNVYHNGEISDEVAALL"
     gene            1295..1822
                     /locus_tag="PXD66_000628"
     CDS             1295..1822
                     /locus_tag="PXD66_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318956.1"
                     /translation="MKLVKFVPALAATLVLAACSNANTQSAPQQAQDAVTKVAEQATQ
                     QVNEIAGNAPRASGAVKVEKATDGAKSIVYRCQSGKRVTANYVFQGEEAKAVNLLVQS
                     GGKTQKIPTLTRDASNQDSTSFKTDKYSWDLDTGFTLSSNGQSGMLTKYGAKTDTIVA
                     KLCDVDKALTSKLNK"
     gene            1889..2467
                     /gene="ruvC"
                     /locus_tag="PXD66_000629"
     CDS             1889..2467
                     /gene="ruvC"
                     /locus_tag="PXD66_000629"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HDL6318957.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQNGRQLEYLGSGAIRTTAEDLPTR
                     LKRIYAGVSEIITQFQPNMFAIEQVFMAKNPDSALKLGQARGTAIVAAVNHDLPVFEY
                     AARLVKQTVTGIGSADKVQVQDMVTRMLKLSAKPQADAADALAIAITHAHSIQHSLIV
                     AKQSQQNVSSEKEQILALMKTRYSRGRFRLKG"
     gene            complement(2533..3813)
                     /gene="murA"
                     /locus_tag="PXD66_000630"
     CDS             complement(2533..3813)
                     /gene="murA"
                     /locus_tag="PXD66_000630"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDL6318958.1"
                     /translation="MEKFRVYGPFSLNGTVDISGAKNAALPILFAAILAEKTVTLKNV
                     PDLKDVDTTFKILRQLGVTVEKGSDKGVVHIDASKINNYVAPYELVKTMRASIWALAP
                     LVARFQLGQVSLPGGCTIGARPVDMHISGLEKMGAKIELDEGYVKATSNGRLNGARIY
                     MDKVSVGATLSVMMAGTLAKGTTVIENAAREPEIVDTADFLNAMGAKISGAGTDMITI
                     EGVEHLGGCEHSVVPDRIETGTFLVAAAISGGRITCRNTKADTLDAVIEKLREAGMQV
                     DVTENTITLDSLGRRPKAVNIRTMPHPGFPTDMQAQFTLLNTVAEGTSRITETIFENR
                     FMHIPELIRMGAKAEIEGNIAICYGVEQLKSAEVMATDLRASISLVLAGCIATGETIV
                     DRIYHIDRGYEHIEEKLRQIGARIERFSAPFEGE"
     gene            complement(3923..4183)
                     /locus_tag="PXD66_000631"
     CDS             complement(3923..4183)
                     /locus_tag="PXD66_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HDL6318959.1"
                     /translation="MNPSQIEEILKQALSDATEVHAQGENAHFGVIVVSDSIASLSKV
                     KQQQAVYAPLAEYFTTNAIHALTIKVFSVEKWKTERLLNMVG"
     gene            complement(4216..4539)
                     /locus_tag="PXD66_000632"
     CDS             complement(4216..4539)
                     /locus_tag="PXD66_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HDL6318960.1"
                     /translation="MPQKTLQWDIQQNNESLFVKLSGELTRNTLLPLWKQRASFLSPT
                     GKQSLYWDLKEITRIDSAGFTLLAELLNHYHKIIPNSLINTPDSVRTLAALYDLDGWF
                     EQFII"
     gene            complement(4551..5186)
                     /gene="mlaC"
                     /locus_tag="PXD66_000633"
     CDS             complement(4551..5186)
                     /gene="mlaC"
                     /locus_tag="PXD66_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HDL6318961.1"
                     /translation="MLKNIKKIIVTGLVAVSALFSTTAFAETSPYVLMQQTADKLFGD
                     IKANQAKIKSNPEYLRTIVRNDLMPHVHVKYAGQLVLGKNLSAATDAQKEAFFNAFGQ
                     FVEQSYAQVLTQYQDQSVQIESPKSVDGKSIVSIRVNVSGKGAAQPIKLDFKWRKNSK
                     TGEWQAYDMAAEGVSMVATKQNEWAGVIRQKGIDALIAQVAQSAKQPITLK"
     gene            complement(5232..5756)
                     /gene="mlaD"
                     /locus_tag="PXD66_000634"
     CDS             complement(5232..5756)
                     /gene="mlaD"
                     /locus_tag="PXD66_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HDL6318962.1"
                     /translation="MRQSIKYEFWVGLFVLLGLAALVFLGLRVANVQGFSNEKTYTLY
                     ATFDNIGGLKVRAPIKVGGVVVGRVADISLDNKTYTPKVALAVNESFNQIPDTSSLSI
                     KTSGLLGEQYVALNVGFVIEGETAMMKEGDTFVDTNSAMVLEDLIGQFLYGDKKSDKT
                     EDEKASDSAEPKAE"
     gene            complement(5777..6553)
                     /gene="mlaE"
                     /locus_tag="PXD66_000635"
     CDS             complement(5777..6553)
                     /gene="mlaE"
                     /locus_tag="PXD66_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HDL6318963.1"
                     /translation="MVNFISSIGAVVINFIRTLGRSAFMLWGALIGKPEFRKHTPLLI
                     KQLYVLGVQSLLIIMLSGLFIGMVLGLQGYVVLVDFAAESSLGTLVSLSLLRELGPVV
                     TALLFAGRAGSALTAEIGLMKATEQLSSLEMMAVDPLRRIIAPRFWAGVISMPVLAAI
                     FTAIGIWGGSLVGVDWKGVDGGSFWSVMQNSVNTSDLINGFIKSVIFAFAVVWIALFN
                     GYDCVPTSEGISQATTRTVVNASLVILGLDFILTAIMFGG"
     gene            complement(6546..7349)
                     /gene="mlaF"
                     /locus_tag="PXD66_000636"
     CDS             complement(6546..7349)
                     /gene="mlaF"
                     /locus_tag="PXD66_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HDL6318964.1"
                     /translation="MTKTENLVEVKNLTFKRGERVIYDNLNLQVQKGKVTAIMGPSGI
                     GKTTLLRLIGGQILPESGEILFDGRDICKANNKELYEIRQRMGMLFQSGALFTDISTF
                     DNVAFPIREHTRLPEELIRKLVLLKLEAVGLRGAANLMPSELSGGMARRAALARAIAL
                     DPDLIMYDEPFTGQDPISMGVIVELIKELNQALNLTSIVVSHDVKEVLSIADYAYIVA
                     DKRVIAEGTPEILQASEDPQVVQFLAGKADGPVRFHYPAKDYTEELFNG"
     gene            7574..8968
                     /locus_tag="PXD66_000637"
     CDS             7574..8968
                     /locus_tag="PXD66_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HDL6318965.1"
                     /translation="MNKTMKKSFLTAFILGSTIAAMPAYAEATLPTAVNGQAVPSLAP
                     MLEKVRPAVVSIAVEGKTKGDSRRSREIPEEFEFFFGPHADLFGNRSSAPRNFRGVGS
                     GAVINAEKGYVITNNHVIDNADKITVKLEDGREFKAKLVGADPLSDVALIQVEDPKNL
                     TEIKFADSDKLQVGDFTVAIGNPFGLGQTVTSGIISALGRSTGQSDEGYENYIQTDAA
                     VNQGNSGGPLINLNGELIGINTAIISPSGGNAGIAFAIPSNMANSLVTQIIEFGEVKR
                     GMLGIKGGELNADLAKEFNISTQQGAFVSEVLPDSAAAKAGFKAGDVITEINGQKIRS
                     FSELRAKVATSGVGKEIELTYLRDGKEAKAKVTLQSDSEAKVTASNLIPALKGADFNN
                     YNAKGIKGVEISNVEKGSIAEMRSLKKGDIIIGVNRHSVENIADLRKILETKPSVVAL
                     NIVRDGANFYVILQ"
     gene            complement(9020..9781)
                     /locus_tag="PXD66_000638"
     CDS             complement(9020..9781)
                     /locus_tag="PXD66_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C biogenesis protein"
                     /protein_id="HDL6318966.1"
                     /translation="MKTREQLSQENYQQAVKFAQHFADDVRQEFEREAEQRYQFERTQ
                     FEQSVLQNNAQNRPLAKIASLSCVAILLLAMTYYGLSGRYQVVQSGLQQHQDFQQQMS
                     DAHGTSKNERYILSLQNQLRENPNNGDLWYELGQAYALSNDFDSALVCYDNAEKLLGK
                     RAAILGAIATARYYDNGQKMTVEIQAIIEQALQLDKNENASLLLLASDSFLNNNFQQA
                     LDNWRKVLDSNNDSINRRAIIQSMEMARQMLNSQQ"
     gene            complement(9768..10220)
                     /gene="nrfF"
                     /locus_tag="PXD66_000639"
     CDS             complement(9768..10220)
                     /gene="nrfF"
                     /locus_tag="PXD66_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HDL6318967.1"
                     /translation="MRNSIISIGFLQKIRKILPLVFGLLLFVGTVQAEMVDTFQFQNE
                     ADRVRAISLARSLRCLQCQNQNLVESNATAAYNMRIEVYEMVNQGKSNEEIIRIMTER
                     FGSFVNYDPPFTPQTWVLWGLPVGLFSLLFLAILWRTRKNKGNKNENA"
     gene            complement(10210..10743)
                     /locus_tag="PXD66_000640"
     CDS             complement(10210..10743)
                     /locus_tag="PXD66_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HDL6318968.1"
                     /translation="MKKILLLIPLLLLVVIVGFLTVPLMNKDALAPTEDWQGKPFPEF
                     VGKNLLDSNAHLNNNSLPKEPYILNVWVSWCTWCIKEFPILLELKQKGVPIVGLTYSD
                     RPNDALNALKNWGNPFSLIIDDYEKGFLIQTLKVSSAPSSYLIDKHGVVRYQQKGYNP
                     DFAQDFLPKLEALRNEK"
     gene            complement(10740..12644)
                     /gene="nrfE"
                     /locus_tag="PXD66_000641"
     CDS             complement(10740..12644)
                     /gene="nrfE"
                     /locus_tag="PXD66_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfE"
                     /protein_id="HDL6318969.1"
                     /translation="MLPELGYFSLLLAAISAIFQVGFALLGEVRKQYHWLALAPLFTY
                     LQAAFTTFSFSTLAYAFLTDDFSVIYVAAHSNSQLPDFFKFAATWGGHEGSMLFWLTA
                     LVIWSAVFCKFSQKIDRLFANRTLAMLGLIALGFLLFILLVSSPFERGFPPPPEGRDL
                     NPMLQDIGLIFHPPLLYLGYVGFAVSFAMIVAALVSGGMDAAIMRWLRPWTMISWGFL
                     TAGIILGAWWAYYELGWGGWWFWDPVENASLMPWLLGTALVHSLIVSQQRGIFYYWTI
                     LLAIFAFALSLLGTFIVRSGVLTSVHAFAVDPDRGMALLILFFGLSFIALALFAFRAN
                     LWQGKVRFNLLSKETAFLLINGLFSVAASVVLLGTFYPMIFTVMNWGSISVGAPYFNS
                     VFTPLALLLMIVMGFAVVLGWKQISNKQILVKLWLLPIAVGLALGLIYASISQREHFA
                     LQPLPTIFISLAIWIILTHLPYSQKMWQIRPLAMRLAHIGFAVCVIGAMMNSYYGDEI
                     GVRLKPNQAAELAGYTFKYEDYHDLIGPNYTAEQAVFSISRDEQLLATVTPERRYYDV
                     RTMTMAEVGLFHHNLDDIYIVMGDKFGNLEYAFRLHYKPYVQALWLGGMMMVMAALLA
                     LFGYRRENKK"
     gene            complement(12914..13861)
                     /gene="nrfD"
                     /locus_tag="PXD66_000642"
     CDS             complement(12914..13861)
                     /gene="nrfD"
                     /locus_tag="PXD66_000642"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HDL6318970.1"
                     /translation="MNEYVPFQTPNLVWDGTIAIYLFLLGISSGAVQLAIAYRNSGVK
                     MEKPSQNWIIRSAAILGTLPTIIGLLLLIFHLTKPWTFWKLMFNYNFTSVMSMGVMLF
                     QLYMAVLVVWIAIMFKDWIVRYLPVVSGLIDFAEAKLLKPIEFILFVLAAVLGAYTGF
                     LLSALVSYPMLNNPVLPALFLASGASSGIAATFLVILLAGKLSGESHEVHFMHKFEVP
                     IMVTELGLIVAFFVGLHFGGADKSLALTHALNGFWGNVFWIGVMLIGIAIPLVANIFG
                     SHRLKHNVKFIILISVFDLIGVLCLRYFILYAGQLTVAS"
     gene            complement(13861..14538)
                     /gene="nrfC"
                     /locus_tag="PXD66_000643"
     CDS             complement(13861..14538)
                     /gene="nrfC"
                     /locus_tag="PXD66_000643"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007524349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HDL6318971.1"
                     /translation="MTCTRRDFVSGAGAIAVVASTGLATSITLATEKEQKKIRYAMVH
                     DETACIGCTACMDACRTTNKVPEGVSRLEIIRSEPFGEFPNVQYEFFRQSCQHCTNAP
                     CVSVCPTGASFIDPETGIIDVHSDLCVGCQYCIAVCPYRVRFIHPEKKSADKCNFCRD
                     TNLAEGKQPACVEACPTKALTFGDMNDPSSDIFHKVKNNPVYRTKTALGTEPNLYHIP
                     FGKGEHR"
     gene            complement(14538..15194)
                     /gene="nrfB"
                     /locus_tag="PXD66_000644"
     CDS             complement(14538..15194)
                     /gene="nrfB"
                     /locus_tag="PXD66_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HDL6318972.1"
                     /translation="MKKLISKAGRAVALIQAVVFAGFFAISSANALMPEQPKPLWQSE
                     TADDAKVAQDARRDPNKYCTQCHESANATGKPFHHAGKHFQKDVKSPNNGEPLTCISC
                     HGNISESHRKGAKDVMRFNPHGKASNPSLERSVNEQNQVCFACHTAEKLREKFWAHDT
                     HATKIACTNCHEIHPEKDPMKDIPEKDRIKLCTDCHTKIHSGEFKNPEMKATEAKGTK
                     "
     gene            complement(15213..16715)
                     /gene="nrfA"
                     /locus_tag="PXD66_000645"
     CDS             complement(15213..16715)
                     /gene="nrfA"
                     /locus_tag="PXD66_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HDL6318973.1"
                     /translation="MKFLIKSLAVATISILGCLQTALAEEAKTESLTDKAVKHEKLGV
                     KIESANHLFAEKYPLQYDSWKSTAKSTDRGSALEADPRYVILWAGYAFAKDYNKPRGH
                     FYAVTDVRDILRTGAPKDENDGPQPMACWTCKGPDVPRLIEEKGERGYFDPKWAKYGA
                     EIVNSIGCADCHDTTSEEFKQGKPALRVARPHVLRALNTVGWKFEDLDKHGKRPAVCA
                     NCHVEYYFKNKTDVTFPWDKGVDVDSIEKYYDEINFTDWTHALSKAPMLKTQHPDFEV
                     WSQGTHGKNGVTCIDCHMPKVKDKDGKVYTDHKIGNPFDQFEATCKTCHEQSKETLEK
                     RVKEYKHEVKEAMIRLEDQLVKAHFEAKAAWEAGATQEEMKEILMAIRHAQWRWDYSA
                     AGHGNHFHAPDVMLRTIATGIDRAADARAKLGVVLAKHGVTTPVAIPDISTKEKAQLA
                     IGLDIPKEQAAKDEFLKTVVPQWEKEARAKGLLPAEEADKPVAAPKAEAK"
BASE COUNT         4988 a         3713 c         3326 g         4693 t
ORIGIN      
        1 tgtggtgatt tatgctaaaa atacccaacg agtattgatg ctacaacgca aggacgatcc
       61 tgatttttgg caatcggtga ccggttcgat caatattgga gaaacaccgc tggaagcagc
      121 agaacgggaa gtgagcgaag aaaccggttt gcaaactttt gagcaaaaac aaccgcttgt
      181 tgattgccaa aagcagatag aatttgaaat tttcccgcat tttcggtata aatacgcacc
      241 taatatcaca cattgtgtcg aacattggtt tttgctgcca cttgatagtg agcaagagcc
      301 tattttaacc gaacatttgg catatcgttg ggtttcagtg gaagatgcgg ttcgactgac
      361 taaatctccc aacaatgccc aagcaataca agaatattta acatcaaaat aaggaaacaa
      421 cagaatggca ggccatagta agtgggcaaa tattaaacac cgtaaagcgg cacaagatgc
      481 acaacgcggt aaaattttta ctaaattaat tcgtgaattg gtgactgccg ccaaactcgg
      541 cggtggtgat acctcttcca accctcgttt gcgtgcggcg gtggataaag cgttatccag
      601 caatatgacc cgtgatacca tcaaccgtgc aatcgagcgt ggtgtaggcg gtggcgatga
      661 taccaatatg gaaaccaaaa tctacgaagg ttacggcccg ggtggtacag cagtgatggt
      721 ggaatgttta agtgataacg ctaaccgtac gatttcccaa gttcgtccaa gtttcacaaa
      781 atgtggcggt aacctaggta ctgagggttc ggtaggttat ttatttagca aaaaaggctt
      841 aattttagtg gcaaatgccg atgaagatgc cttaactgaa gctgctattg aggccggtgc
      901 ggacgatatt caaccacaag aagatggtag ctttgaaatt tataccgctt gggaagattt
      961 aggctcagtg aaagacggca ttgaggctgc aggctttaaa attgagaatg cagaagtaac
     1021 aatgattcca tcaactaccg ttgaacttga cgcagaaact gcaccaaaac ttcttgattt
     1081 aatcaaccgc cttgaagatt gtgatgatgt acaaaacgtt tatcacaatg gtgaaatcag
     1141 tgacgaagtc gctgcattac tgtaataaaa aaatcacgca aaataagcat aatttaggtt
     1201 atgcttattt tttatgaact tttttcctat attactgact aacctaatgt agtttatatg
     1261 ctactacttt actataacat tttaaggaga gtctatgaaa ttagtgaaat ttgtccctgc
     1321 cctagcagca actttagtat tagccgcttg ctctaatgca aacacacaat ctgctcctca
     1381 gcaagctcag gacgctgtga ctaaagtggc tgaacaagca acacagcaag tgaatgaaat
     1441 tgcaggtaat gcacctcgtg caagtggtgc agtaaaagta gaaaaagcaa ctgatggtgc
     1501 aaaatcaatt gtatatcgtt gccaaagtgg taaacgtgta actgcgaatt atgtcttcca
     1561 aggagaagag gcaaaagcag taaatctatt agtccaaagc ggcggtaaaa cacaaaagat
     1621 cccaacatta actcgtgatg cctcaaacca agattctact tcattcaaaa cggataaata
     1681 ttcttgggat ttagacacag gctttacctt atcatctaat ggccagtcag gtatgctaac
     1741 caagtatggt gctaaaacag ataccatcgt tgctaaatta tgtgatgtag ataaagcatt
     1801 aacctcaaaa ttaaataaat aatctttctt gattttacta ctcataagcg agaagataat
     1861 atcttctcgc ttattttatt gcacaaagat ggctattatt ttaggaattg accccggctc
     1921 aagggtaaca ggttacggtg tgattcgcca aaacgggcga cagctggaat atttaggaag
     1981 tggggctatt cgaaccacgg ctgaagatct gcctacccgc ttaaaacgga tttatgcagg
     2041 cgtgagtgaa atcatcactc aatttcaacc aaatatgttc gccatagagc aggtatttat
     2101 ggcaaaaaac cctgattctg ccttaaagct cgggcaggct cgaggaacag cgattgtagc
     2161 agcggttaat cacgatttac ctgtgtttga atatgccgcc cgattagtca aacaaaccgt
     2221 tacgggaatt ggttctgctg ataaagtaca agtgcaagat atggtaaccc gtatgcttaa
     2281 actctccgcc aaaccacaag ccgatgcggc tgatgcatta gccattgcga ttacccacgc
     2341 tcattcaatc caacattcac tgattgttgc taagcaaagc cagcagaatg tgagtagtga
     2401 gaaagagcaa attttagcac taatgaaaac ccgttatagc cggggaaggt ttaggttaaa
     2461 aggctaactc aatataaaat gaccgcttgg aactaatgtt tcaagcggtc attttttgag
     2521 aaaattttac aattactcgc cttcaaaagg agctgagaaa cgttcaattc tcgccccaat
     2581 ctgacgtaat ttttcttcga tgtgttcata gccacggtct atgtggtaaa ttctatccac
     2641 aatcgtttca cccgttgcaa tacagcctgc caacactaag ctgatggaag cacgcaagtc
     2701 agttgccatt acttctgctg attttaactg ctctacacca tagcaaattg caatattacc
     2761 ttcaatttcc gctttggcac ccatacgaat cagctcagga atatgcataa aacggttttc
     2821 aaaaatcgtt tctgtaatac ggcttgtgcc ttctgcaact gtatttaata aggtaaattg
     2881 agcttgcata tcagttggga aacctgggtg tggcatagtg cgaatgttca ctgctttcgg
     2941 gcgacgacct agagaatcaa gcgtgatagt attttccgtc acatcaacct gcattccagc
     3001 ttcacgtaat ttttcgatca ctgcatcaag agtgtctgct ttggtgttac ggcaagtaat
     3061 tcgaccaccg gaaatagccg ctgccactaa aaatgtaccg gtttcaatac gatccggcac
     3121 tacgctgtgt tcgcaaccgc ctaaatgctc aaccccttca atggtaatca tatcggtacc
     3181 tgcaccagaa attttagctc ccatcgcatt taagaaatcg gcagtatcta caatttcagg
     3241 ctcacgagca gcattttcaa taacagtagt accttttgct agagtacctg ccatcatcac
     3301 cgataaagtt gctcctacgc tcactttatc catatagatt cttgcaccgt ttaaacgacc
     3361 gtttgaagtt gctttgacat agccttcatc aagctcgatt ttcgccccca ttttttcaag
     3421 accggaaatg tgcatatcta ccggtcttgc cccgatagta caaccgcccg gtaaagaaac
     3481 ttgcccaagt tggaaccggg caactaatgg agctaacgcc caaattgaag cacgcattgt
     3541 tttcactaat tcgtaaggtg caacgtaatt attgatctta ctcgcatcaa tatgtaccac
     3601 gcctttatcg ctaccttttt caaccgttac ccctaattgg cgtaagattt taaaagtggt
     3661 gtccacatct tttaaatcag gcacattttt taacgtaact gttttttcag ccaaaatagc
     3721 ggcaaataaa atagggagtg cggcattttt cgcacctgaa atatcgacag taccatttaa
     3781 agaaaatgga ccatacacac gaaatttttc catttgggag atcctaaaaa ataataattt
     3841 gtaggggcga aaaatttttc gcccaatttt ccaacgttta caaaagagtt tgggcgaata
     3901 gtgattcgcc cctacatttc atctatccaa ccatatttag caaacgctca gttttccatt
     3961 tttccacgct gaatacttta atggttaaag cgtgaatcgc attagtagtg aaatactcag
     4021 ctaaaggggc ataaacagct tgctgttgtt ttacttttga aagagaagca atgctgtcac
     4081 ttactacaat aacgccaaag tgggcattct cgccttgagc gtggacttct gttgcatcag
     4141 ataatgcttg ttttaaaatt tcttcaattt gtgatggatt cattctgact tcctaaaata
     4201 tgattggtaa ctcactcaaa taataaattg ctcaaaccaa ccgtctaaat cgtataatgc
     4261 agccaaagtt ctgacagaat ctggcgtatt aatcaaactg tttggtatga ttttatggta
     4321 atgattcagt aattcagcaa gtaaggtaaa accggcagaa tcaatccgag tgatttcttt
     4381 taaatcccaa tataggcttt gtttccctgt tggagataaa aaagaagcac gttgcttcca
     4441 taaagggagc aacgtgttac gagttaattc gcctgatagt ttcacaaaaa gactttcgtt
     4501 attttgctga atgtcccatt gtaatgtttt ttgaggcata actaaactac ttattttaaa
     4561 gtgataggct gttttgcaga ttgtgccact tgtgcaatta aggcatcaat ccctttttga
     4621 cgaatcacac ccgcccattc gttttgcttg gttgcaacca tacttactcc ttcagcggcc
     4681 atatcgtatg cttgccattc acctgttttg ctgtttttac gccatttaaa atctaatttg
     4741 atcggctgtg cagcaccttt acctgacacg tttacacgaa tgcttacgat agatttacca
     4801 tccaccgatt tcgggctttc aatttgcaca ctttgatctt gatactgggt taacacctga
     4861 gcataagact gctcaacaaa ttgaccaaaa gcattaaaga atgcctcttt ttgtgcatca
     4921 gtcgcagccg ataagttttt ccctaacact aattggcctg catatttcac gtgaacgtgt
     4981 ggcattaaat cgttacgcac aatggtacgc aaatattcag ggtttgattt aattttcgct
     5041 tgatttgctt taatatcgcc aaataattta tctgcggttt gttgcattaa tacataaggg
     5101 ctagtttcag caaatgcggt ggttgaaaat aatgcagaaa ctgccactaa gcctgttacg
     5161 ataatttttt taatgttttt taacattatt gatgctctcc aaaactctaa attagaggtt
     5221 gttgtactta attactctgc ttttggttca gcagaatcac ttgctttttc atcttcagtt
     5281 ttatctgact ttttatcacc atataagaac tgaccgatta aatcttctaa caccattgca
     5341 gaattggtat ctacaaaagt atcaccttct ttcatcattg cagtttcacc ttcgattacg
     5401 aagccaacat ttaacgcaac gtattgctcg cctaataagc ctgaggtttt aattgagagc
     5461 gagctggtat cgggaatttg attaaaactt tcatttaccg ctaatgccac tttcggggtg
     5521 taggttttgt tatcaagtga aatatcagcc actcggccaa ctaccacacc gccaacttta
     5581 ataggcgcac gaacctttag tccaccaata ttatcaaaag ttgcatacag ggtgtaagtt
     5641 ttctcatttg agaagccctg cacatttgct actcttaagc ctaaaaagac aagagctgca
     5701 agcccaagta gtacaaataa gcctacccaa aattcatatt taattgattg acgcattgtc
     5761 tgttcctttc aaaaaatcaa ccgccgaaca ttatcgcagt taaaataaaa tccaatccta
     5821 aaatcaccag tgaagcattt accaccgttc ttgtggtcgc ttggctgatt ccttctgaag
     5881 tcggtacgca atcgtagccg ttaaataagg caatccagac caccgcaaag gcaaaaatca
     5941 cacttttaat aaagccgtta attaaatcgc tggtatttac cgagttttgc attaccgacc
     6001 aaaaactgcc accatcaacg cctttccaat ctaccccgac taatgagccg ccccaaattc
     6061 cgatagcggt aaaaatagca gctaacaccg gcattgaaat cacgcctgcc caaaagcgag
     6121 gagcgatgat acgtctaagc ggatcaaccg ccatcatttc aagactggaa agttgctctg
     6181 tggctttcat cagcccgatt tctgcggtca atgccgaacc ggctcgccct gcaaataata
     6241 atgcagtaac tacaggacca agttctcgta aaagcgagag tgaaaccaaa gttcccaagc
     6301 tactttcggc ggcaaaatcg accaatacca catagccttg taagcctaac accattccga
     6361 taaataagcc cgacagcata ataattaaga gcgattgcac tcctaacaca taaagctgtt
     6421 taatcagtaa cggggtatgt tttctaaatt caggtttacc gattaatgct ccccataaca
     6481 taaaagcgga acgccctaat gtacggataa aattaatcac caccgccccg atagagctaa
     6541 taaaattaac cattaaatag ctcctccgta taatcttttg ctggataatg gaaccgcacc
     6601 ggaccatcag ctttaccggc taagaattgt accacttgcg gatcttcgct tgcctgtaaa
     6661 atttccggtg taccttcagc aatcactcgt ttatctgcca caatataagc ataatcggca
     6721 atacttaata cttctttcac atcgtgagac accacaatgg aggtcaaatt caatgcctga
     6781 tttaactctt taatcagctc gacaatcaca cccatactaa tcggatcttg cccggtaaac
     6841 ggctcatcat acataatcag atccggatct aacgcaatcg ctcgagcaag ggcggcacgt
     6901 cttgccatac cgccggaaag ttcggacggc attaaatttg ccgcacctcg caacccaacc
     6961 gcctcgagct tcaataacac caatttgcga attagctctt cgggtaaacg ggtatgctcc
     7021 cgaatcggga aagccacatt atcgaaggta gaaatatcgg taaataacgc ccccgattga
     7081 aacaacatcc ccattcgttg gcgaatttca tacaactctt tgttattggc tttgcaaata
     7141 tctctgccat caaataaaat ttcgcccgat tcaggtaaaa tctgcccacc gattagcctc
     7201 aacagggtgg ttttaccaat cccagaaggc cccataatgg cagtcacttt acctttctgc
     7261 acttgcagat tcaggttatc gtaaatcact cgctcccctc gcttgaaggt caagtttttt
     7321 acttcaacca aattttccgt ttttgtcatc aaataggact caaattaata aaagtgatat
     7381 ttatggtcta tcaaatttgc cagaatgtca aaaaactttt aataatctga caaaaatctg
     7441 cctttacaag cggtcagatt tgctaaattt tttgcaaacc ggctgaactg aaaaagaaag
     7501 gtcttgtctt agccaatacg agcgttttca ctcgtagatt ataaaaacat aggatatgag
     7561 gaacattcac aatatgaaca aaacaatgaa aaaatcattt ttaactgcat tcattttagg
     7621 ctcaaccatt gccgcaatgc cggcgtatgc agaagcaaca ttacccacag ccgtaaatgg
     7681 gcaagctgtg ccaagccttg caccaatgct ggaaaaagtt cgcccggcgg tggtgagtat
     7741 tgcagtagaa ggtaaaacca aaggcgatag ccgccgcagt cgtgaaattc cggaagaatt
     7801 tgaatttttc ttcggtcctc acgccgattt attcggcaac cgctcatcag ctccacgcaa
     7861 tttccgtggc gttggatcag gtgcagtcat caatgccgaa aaaggttatg tgattaccaa
     7921 taaccacgtg atcgacaatg ccgataaaat taccgtaaaa cttgaagacg gacgtgaatt
     7981 taaagctaaa ttagttggtg ctgatcccct ttcagatgtg gcgttaattc aagttgaaga
     8041 tccaaaaaac ttaactgaaa ttaaattcgc cgattccgat aaattacaag tgggcgattt
     8101 tacagttgca atcggtaacc cgtttggctt aggacaaact gttacttcag gcatcatctc
     8161 ggcacttggt cgttcaaccg gtcaatccga tgaaggctat gaaaactata ttcaaaccga
     8221 tgcggcagtt aaccaaggta actctggcgg cccgttaatt aacttaaacg gcgaattaat
     8281 cggaattaac accgctatta tttctccaag tggtggcaat gccggtatcg cctttgcgat
     8341 tccaagtaat atggcaaaca gcttggtgac tcaaattatt gagtttggtg aagtaaaacg
     8401 tggaatgtta ggcattaaag gcggtgaatt gaatgctgac cttgctaaag agttcaatat
     8461 ttccacccaa caaggggcat ttgtaagcga agtcttaccg gattctgctg ctgctaaagc
     8521 cggctttaaa gcaggtgatg tgattactga aatcaacggt caaaaaatcc gtagtttcag
     8581 cgaattacga gcaaaagtag ctacttcagg tgtaggtaaa gagattgaac ttacttacct
     8641 gcgtgacggc aaagaagcca aagcaaaagt gaccttacaa tcggattctg aagccaaagt
     8701 tactgcttca aacctcattc cggcattaaa aggggcagat tttaacaact acaatgccaa
     8761 aggcattaaa ggggttgaaa tttctaacgt agaaaaaggc tcgattgctg aaatgcgaag
     8821 cctcaaaaaa ggcgatatta tcatcggcgt gaaccgccac tcggtagaaa acattgccga
     8881 tttacgcaaa atattagaaa ccaagccatc tgtggttgca ctcaatattg tgcgtgacgg
     8941 tgcaaatttc tatgtgattt tgcaataaac cttttgatcc actcaaacaa aagccgatga
     9001 caaaaatcat cggcttttgt tattgctggg aatttaacat ctgtcgtgcc atttccatac
     9061 tttggataat cgctctgcgg ttgatagaat cattattgct atctaacact ttacgccaat
     9121 tatcgagagc ttgttgaaaa ttgttattta aaaagctgtc ggaggctagc aggagtaaac
     9181 tggcattttc atttttatcc aactgtaggg cttgctcgat aatggcttga atttcaacgg
     9241 tcattttctg cccgttatcg taataacgag cggtggcaat cgcccccaaa atagccgctc
     9301 gttttccgag tagcttctcg gcattatcgt agcaaaccag tgccgaatca aaatcattgc
     9361 ttaacgcata agcctgccct agttcgtacc ataaatcacc gttattcggg ttttctcgaa
     9421 gctggttttg cagactcaaa atatagcgtt catttttcga tgtgccgtgt gcatcgctca
     9481 tctgttgttg aaaatcttga tgttgttgca aacctgattg caccacttga taacgtccgg
     9541 aaagcccata gtaagtcatt gctaacaaca aaatcgccac gcaactcagg cttgcgattt
     9601 tagcaagcgg tcgattttga gcattatttt gcaaaacgct ctgttcaaat tgcgttcgtt
     9661 caaattggta acgttgctct gcctcacgct caaactcttg tctgacgtca tctgcaaaat
     9721 gttgggcaaa tttaaccgct tgttggtagt tttcttggct taattgctca cgcgttttca
     9781 tttttgttgc ctttattttt gcgagttcgc cacaaaatag ctagaaataa caggctaaac
     9841 aagccaaccg gcaagcccca taatacccat gtctgtggcg tgaatggtgg atcgtaattc
     9901 acaaaactgc cgaaacgctc ggtcataatg cggataattt cctcattact tttcccttga
     9961 ttcaccattt catacacttc aattcgcata ttgtaagccg ccgtcgcatt ggattcgacc
    10021 aaattttggt tctgacattg caaacatcgc aacgaacgag ccagtgaaat cgcccgtacc
    10081 cgatccgcct cattttgaaa ttgaaaggtg tccaccattt cagcttgaac cgtgccgaca
    10141 aatagcaaca agccaaatac aagcggtaat attttgcgga ttttttgcaa aaagccgatg
    10201 gaaattatgc tatttctcat ttctgagagc ctctaatttg ggtaggaaat cctgtgcaaa
    10261 atcagggttg taaccctttt gttggtaacg cactacaccg tgtttatcaa tcaaataact
    10321 ggaaggtgca gaagagactt tcagggtttg gatcaaaaag cctttttcgt aatcatcaat
    10381 aattaaacta aacggatttc cccagttttt caatgcgttg agggcatcat ttgggcggtc
    10441 gctataagtt aagccgacaa tcggcacgcc tttttgtttt agttccaaca aaatcgggaa
    10501 ctctttaatg caccaagtac accagctcac ccacacattg agaatataag gctctttcgg
    10561 caagctgttg ttatttaaat gcgcgttact atcaagtaag ttcttgccta caaactctgg
    10621 aaacggcttg ccctgccaat cttcagtggg agcaagggca tctttattca ttaacggcac
    10681 agtcaaaaag ccaacaataa ctacaagtaa caggagcgga ataagtaaca ggattttttt
    10741 cattttttgt tttctctacg ataaccgaat aatgccaaca gggcggccat taccatcatc
    10801 ataccaccta accataaggc ttgcacataa ggcttatagt gcaaacggaa ggcatattct
    10861 aaattgccga acttatcccc catcacaata taaatatcgt ctaaattgtg gtggaaaagc
    10921 cctacttctg ccattgtcat cgttcttaca tcataatagc gtctttccgg tgtgacggtt
    10981 gccagcaact gttcatctcg gctgatagaa aaaacggctt gctcggcggt gtagttcgga
    11041 ccgatgagat cgtgataatc ttcatattta aaggtataac cggcaagttc cgctgcttga
    11101 ttcggcttta accgcacgcc gatttcatcg ccgtaataac tgttcatcat tgccccaatc
    11161 acgcaaaccg caaagccgat atgggctaac cgcatcgcaa gcggtcgaat ttgccacatt
    11221 ttttgcgaat agggcaaatg agtgagaata atccagatcg caaggctgat aaatatggtg
    11281 ggtaacggct gcaaagcaaa atgctctctt tgcgagatgg aggcataaat caaccctaac
    11341 gctaaaccga ctgcaatcgg cagcaaccaa agttttacca atatctgctt atttgaaatc
    11401 tgtttccagc ccagcaccac cgcaaagccc attactatca ttaacagtaa cgctagtggc
    11461 gtgaatacgc tattgaaata gggggccccc actgaaatcg atccccaatt catcactgtg
    11521 aagatcatcg ggtagaaagt ccccagcaat accacagaag cagccacact aaataagccg
    11581 ttaatcaata aaaatgccgt ttctttagaa agtaagttga aacgcacttt gccttgccag
    11641 agattcgctc ggaatgcaaa cagagcaagg gcaatgaaac ttaagccgaa gaataagatc
    11701 agcagtgcca ttcctctgtc gggatcgacc gcaaaggcat gaaccgaggt cagtacaccc
    11761 gaacgaacaa taaacgtgcc taataggctt aaagcaaagg cgaaaatggc aagtagaatc
    11821 gtccaataat agaaaatgcc acgctgttgg ctcacaatta aactatgcac caatgcggtg
    11881 ccaagtagcc aaggcattag ggaggcattt tccaccggat cccaaaacca ccagccaccc
    11941 cagccgagtt cgtaatatgc ccaccacgcc cctaaaataa tgccggcggt gagaaatccc
    12001 cacgaaatca tcgtccacgg acgaagccaa cgcataatcg cagcgtccat tccgcctgaa
    12061 acgagggcgg cgacaatcat tgcaaagctc accgcaaagc cgacatagcc caagtagagc
    12121 aaaggcgggt gaaagatgag cccaatatct tgcaacattg ggttgagatc tcgcccttca
    12181 ggtggtggtg gaaagccacg ttcaaatggg ctggaaacca atagaatgaa aagtagaaaa
    12241 cctagtgcaa ttagccctaa catcgctaag gttcggttgg caaacaagcg gtcaattttt
    12301 tgcgaaaatt tgcaaaatac cgcagaccaa atcacaagag cggtgagcca aaacagcatt
    12361 gagccttcgt gtccgcccca agtggcggca aatttaaaaa aatcggggag ctggctgttg
    12421 gaatgagcgg caacgtagat aaccgaaaaa tcatcggtta gaaaagcata agctaaggta
    12481 gaaaaggaaa atgtggtaaa agcagcttgc aaataagtga aaagcggagc caatgccagc
    12541 caatgatatt gcttgcgaac ttcgcctaga agtgcaaaac caacttgaaa aatggcacta
    12601 attgcagcca ataaaagtga aaaatagccg agttccggta gcataaaaaa tccaaaaaat
    12661 gaccgcttgt gaatgtaaaa aaagccctct ccacttgtgg gagagggaca gatttttctt
    12721 cgttcagaag aaaaatcagg gagaggggaa ttaaataatt ttgctaaaaa taacaatact
    12781 ccctctctct gaaaaatatt tctgaacgaa atatttttcg gtctcaatac tcgctacgct
    12841 cgctccttcg gagccgttgg caaagccaac gttcaaatgt aaacatttgt cccacaaggg
    12901 gagagagtat atcctaactt gcaaccgtta gttgccctgc ataaaggata aagtaacgca
    12961 agcagagtac accgattaag tcgaaaacgg aaattaagat aataaatttc acgttatgtt
    13021 tcaagcggtg gctaccaaaa atattggcaa caagcggaat cgcaataccg attaacatta
    13081 cgcctatcca gaacacattc ccccagaagc cgttaagtgc atgggttaaa gcgagcgatt
    13141 tatctgctcc accgaagtgt aagcccacaa agaacgctac aattaagcct aattcggtta
    13201 ccataatcgg cacttcaaat ttgtgcataa agtggacttc gtggctttca ccggataact
    13261 tgcctgcgag taaaatcact aggaaagttg ctgcaatacc tgacgatgcc cctgatgcca
    13321 agaacaatgc cggtaacaca gggttattca gcattggata actcactaac gccgagagta
    13381 agaaaccggt gtaagcacca agtacagcag ccaatacgaa taagataaat tcaattggtt
    13441 ttaacagctt cgcttcggca aaatcgatca aaccagagac cacaggtaga taccgaacaa
    13501 tccaatcttt aaacattatg gcgatccaaa ccaccaatac tgccatataa agttggaata
    13561 acattacccc catcgacatt actgaggtaa agttatagtt gaacattaat ttccagaatg
    13621 tccaaggttt ggttaagtgg aaaatcagta acagtaagcc gataatggtt ggtaatgtgc
    13681 caagaatagc cgcactacga ataatccagt tttggcttgg tttttccatt ttgacaccgc
    13741 tattacggta agcaatcgct aactgaaccg cacctgatga aataccaagt aggaataagt
    13801 aaatagcgat agtgccatcc caaacaaggt ttggggtttg gaaaggtaca tattcattca
    13861 ttatctatgt tctcctttac caaatggaat gtgatataaa ttcggctccg tgcctaatgc
    13921 cgttttggtg cgataaaccg gattattttt cactttatgg aaaatatcgc tacttggatc
    13981 attcatatca ccaaaggtta aagctttagt cggacaagcc tctacacaag caggctgttt
    14041 gccttctgca agattggtat ctcggcaaaa gttacattta tccgccgatt ttttctcagg
    14101 gtgaataaaa cgtacacgat acgggcaaac cgcaatacag tattggcaac cgacacacaa
    14161 atctgagtgt acatcaataa tcccggtttc aggatcgata aatgaagcac cggtcgggca
    14221 aacactcaca caaggtgcat tggtacagtg ttggcaagat tggcggaaaa actcatattg
    14281 tacgttcgga aactcaccga aaggctcact acggataatt tctaaacgag aaaccccttc
    14341 cggcactttg ttggtggtgc gacaagcgtc catacaagcc gtacagccga tacaggcagt
    14401 ttcatcatga accatcgcat agcggatttt cttttgttct ttctcagtag caagagttat
    14461 ggaggtggca agccctgtac ttgccacaac tgcgatagca cctgcccctg agacgaaatc
    14521 tctacgggtg caagtcatta ttttgttcct tttgcttctg tggctttcat ttcaggattt
    14581 ttaaattcgc ctgaatgaat tttggtatga caatccgtac aaagtttaat gcgatctttt
    14641 tcaggaatgt ctttcatcgg atctttttcc gggtgaattt catgacagtt ggtacaagca
    14701 attttagttg cgtgcgtgtc gtgagcccag aatttttcac gcaatttctc tgccgtatgg
    14761 caagcaaaac agacttgatt ttgctcattt accgaacgtt caagtgatgg gttacttgct
    14821 ttaccgtgtg ggttaaaacg cattacgtct tttgcccctt tgcggtggct ttcagaaata
    14881 ttaccgtggc aacttataca agtgagtggc tctccattat tcgggctttt cacatctttt
    14941 tggaaatgtt tgcctgcgtg atggaaaggc ttacctgttg cgtttgccga ttcgtgacat
    15001 tgagtacaat atttatttgg atcacggcgt gcatcttgag ctacttttgc atcatcagcc
    15061 gtttctgatt gccaaagagg ttttggctgc tcaggcatta acgcattcgc actggaaatt
    15121 gcaaaaaaac ctgcaaatac gaccgcttgt atgagggcga ctgctcgtcc tgcttttgag
    15181 attaattttt tcatagttca cctcaagcta cattattttg cttctgcttt tggtgctgct
    15241 accggtttgt ctgcttcttc tgcgggtaat aaacctttag cacgagcttc tttttcccat
    15301 tgaggaacca cagtttttaa gaactcatct tttgctgctt gctctttagg aatatctaaa
    15361 ccaatagcaa gttgtgcttt ctctttagtt gagatgtccg gaatcgccac tggcgttgtt
    15421 acaccgtgtt tcgctaacac tacacctaat ttagcacggg catctgctgc acggtcgata
    15481 cctgttgcga tggtacgtaa cattacatca ggtgcgtgga agtggttacc gtgacctgcc
    15541 gcagagtaat cccaacgcca ttgagcgtga cgaattgcca ttaagatctc tttcatctct
    15601 tcttgagttg ctccagcttc ccaagcagct ttcgcttcaa agtgagcttt cactaattga
    15661 tcttctaagc gaatcattgc ctcttttact tcgtgtttat actcttttac acgtttttcc
    15721 aatgtttctt tgctttgttc atgacaagtt ttacaagtcg cttcaaattg gtcaaacgga
    15781 ttaccgattt tgtggtcagt ataaactttg ccgtctttgt ctttcacttt tggcatatgg
    15841 caatcaatac aggttacacc atttttccca tgtgtacctt gtgaccaaac ttcaaaatct
    15901 gggtgctgcg tttttagcat cggagctttc gataaagcat gagtccagtc tgtaaaattg
    15961 atttcatcgt agtatttttc aatgctatct acatctacac ctttatccca agggaaagtt
    16021 acatctgttt tatttttgaa atagtactcc acatggcagt tagcgcacac agcagggcgt
    16081 ttaccatgtt tatctaaatc ctcaaacttc caaccaaccg tattcaatgc acgtaatacg
    16141 tgaggacgtg caacacgtag tgcaggttta ccttgtttaa actcttctga tgtcgtgtca
    16201 tggcagtctg cacaacctat tgagtttaca atttcagcac catatttcgc ccatttagga
    16261 tcaaaatatc ctcgctcacc tttctcctca attaagcgtg gaacatcagg gcctttacat
    16321 gtccaacaag ccataggttg tggaccatca ttttcatctt taggcgcgcc ggtgcgcaaa
    16381 atatcacgca catcggtaac tgcataaaag tgacctcgtg gtttattata atctttcgca
    16441 aaagcatagc ctgcccacaa aataacataa cgagggtcag cctctaacgc acttcctcga
    16501 tctgtggatt ttgcagttga cttccaagaa tcgtattgta gcggatattt ttctgcaaat
    16561 aggtggtttg cagactcaat tttcacgcct aatttttcat gcttaactgc tttatctgtt
    16621 aatgattcag ttttcgcctc ttcagctaaa gcagtttgta aacaccccaa tattgaaatt
    16681 gttgccacag ctaggctttt tattaaaaat ttcattttgg
//
LOCUS       DANLXF010000033        16632 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.033, whole genome shotgun sequence.
ACCESSION   DANLXF010000033 DANLXF010000000
VERSION     DANLXF010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16632
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.033"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            285..1649
                     /locus_tag="PXD66_000646"
     CDS             285..1649
                     /locus_tag="PXD66_000646"
                     /EC_number="2.6.1.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005820549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HDL6318974.1"
                     /translation="MTIKTPIQAVLASNQYFLDRQDAMESNVRSYPRKLPFAYQKAQG
                     VWVTDVEGNEYLDFLAGAGTLALGHNHPKLIQAVKDVLDSGLPLHSLDITTPLKDAFT
                     EELLSFFPKDKYILQFTGPSGADANEAAIKLAKTYTGRGNVIAFSGGFHGMTHGALSL
                     TGNLSAKNAVQGLMPGVQFMPYPHEYRCPFGIGGEAGAKAVEHYFENFIEDVESGVVK
                     PAAVILEAIQGEGGVVPAPISFLQKVREVTQKHGILMIVDEVQAGFCRSGKMFAFEHA
                     GIEPDIVVMSKAIGGSLPLAVLAIKKEFDAWQPAGHTGTFRGNQLAMATGYVSLKIMR
                     EENLAKNAQERGEYLTNALRELSKELPCIGNVRGKGLMMGIDIVDERQPKDATGAYPR
                     DCDLAVAIQKYCFKNKLLLERGGRGGNVVRVLCAVNITQAECEEFIKRFKQSVAEAVK
                     AVRG"
     gene            1739..3274
                     /gene="ddc"
                     /locus_tag="PXD66_000647"
     CDS             1739..3274
                     /gene="ddc"
                     /locus_tag="PXD66_000647"
                     /EC_number="4.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2,4-diaminobutyrate decarboxylase"
                     /protein_id="HDL6318975.1"
                     /translation="MSNLEKHRQSLFCSDPKSIADYESAMNNAVKAVSDWLKEEKMYT
                     GGSIKELRAKINGFNPTQAGVGVQKSLEHLVEIFLKPSLKVHHPHSLAHLHCPTMVTS
                     QIAEVLINATNQSMDSWDQSPAGSIMEEHLIDWLRQKVGYGKGTSGVFTSGGTQSNLM
                     GVLLARDWAIANHWKKADGSEWSVQADGIPADAMQKVKVVCSENAHFSVQKNMAMMGM
                     GFQSVVTVPSNANAQMDVEALAKTLADLKAEGKIVACIVATAGTTDAGAIDDLKAIRK
                     LADEHQAWVHVDAAWGGALLLSKDFRHFLDGIELTDSITLDFHKHFFQSISCGAFLLK
                     DEANYRFIDYKADYLNSEYDEEHGVPNLVSKSLQTTRRFDALKLWFTVEALGEELYAS
                     MIDHGVYLSKEVEAYINATDGLEMLVPAQFASVLFRVVPQGYPAEFVDTLNQNVADEL
                     FARGEANIGVTKVGEHQSLKMTTLSPIATLENVRALLAQVLAEADRIKDSIVNGTYVP
                     PID"
     gene            3377..3739
                     /locus_tag="PXD66_000648"
     CDS             3377..3739
                     /locus_tag="PXD66_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NirD/YgiW/YdeI family stress tolerance protein"
                     /protein_id="HDL6318976.1"
                     /translation="MKKVLGLTLMAMALSACNSTASNGSSQANTGVAPANVISVKQAL
                     AAADDSYVTVEGTLLNKVGDETYLFKDATGQIRVEIDDEVWAGQHVGSEGNKVRLYGE
                     IDKGFNKTELDVKTLTVL"
     gene            complement(3829..4416)
                     /locus_tag="PXD66_000649"
     CDS             complement(3829..4416)
                     /locus_tag="PXD66_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL6318977.1"
                     /translation="MNHLIIYAHPNPSSFNHAILEKTIAASVPNKVVVRDLYQLNFKP
                     ILDWAEFQGTLSKHYEADVAIEHQYWLEADVITLIYPLWWMGFPAILKGYLDRVLSYD
                     FAYRNGEIESIGLLTGKKMQQFVTLGNSNEKYERKGFLAAFEHTLGKGLFEFCGIRDV
                     KMHYFGNIGLKETDYTAILQQITASCSTILKEQAV"
     gene            4592..5140
                     /gene="napF"
                     /locus_tag="PXD66_000650"
     CDS             4592..5140
                     /gene="napF"
                     /locus_tag="PXD66_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HDL6318978.1"
                     /translation="MTDYPNQSLPRRSFLRGQFLDSLKSQKVSQQGYEPIRPPWANLA
                     NFLEKCTACGNCIQACETQIIVKGAAGYPEIDFSRGECTFCEKCVHSCEAEVFRAVSE
                     QPWSHKIEIQAGCLLKLGTECRSCGDSCEMRVIRFRPSLGGIAEMVLNLESCNGCGAC
                     LSVCPTSAIKIQPLQTQANRDE"
     gene            5133..5399
                     /locus_tag="PXD66_000651"
     CDS             5133..5399
                     /locus_tag="PXD66_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HDL6318979.1"
                     /translation="MSEKLSEQENWYVCSLVVQAKPEKLEEVKAEILKIPHTEIHGEK
                     TDEGKLVVTIESNVHLALSDRIDQVRDIKGVIVVSLISNYIDEQ"
     gene            5438..7936
                     /gene="napA"
                     /locus_tag="PXD66_000652"
     CDS             5438..7936
                     /gene="napA"
                     /locus_tag="PXD66_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005566990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase catalytic subunit NapA"
                     /protein_id="HDL6318980.1"
                     /translation="MELNRRDFMKANAAVAAAAAAGIMIPVKNVQADDTGIRWDKAPC
                     RYCGTGCSVLVGTKDGKVVATQGDPDAEVNRGLNCIKGYFLSKMMYGADRMQEPMLRM
                     KDGKFDKNGDFTPVSWDQAFSIMAEKIKTTIKNDGPNAVGMFSSGQTTIFEGYAKVKL
                     WKGGFRSNTIDPNARHCMASAAVAFMRTFGMDEPMGCYNDIEKTDAFVLWGSNMAEMH
                     PILWSRISDRRLSDNKVKVVVMSTYEHRSFELADTPIIFKPHSDLAILNYIANYIIQN
                     DKVNWDFVNKHTKFKRGETNIGYGLRPEHKLQQNTNAKTAGKMYDSDFEEFKKIVAPY
                     TLEEAHRISGVPKDQLETLAKLYADPNQKLVSFWTMGFNQHTRGVWVNHMMYNVHLLT
                     GKIALEGCGPFSLTGQPSACGTAREVGTFIHRLPADMVVTNPKHREIVENSWKLPAGA
                     IPDVPGFHATAQSRALKDGKMKFLWQMCTNNMQGGPNINEEIFPGWRNPETFIVVSDP
                     YPSVSAVAADLILPTCMWVEKEGAYGNAERRTQFWRQQVKGPANARSDTWQLVEFSKY
                     FKVEEVWPEELLAKAPELRGKTLYEILYRNGKVDSYQVPTNIPGYMNDEADHFGYYIQ
                     KGLFEEYAEFGRGHGHDLADFDTYHKVRGLRWPVVKDEKTGEYKETLWRYREGYDPYV
                     KAGEEVAFYGNADKKAVILGVPYEPPAEAPDEEYDLWFCTGRVLEHWHTGTMTRRVPE
                     LHKAFPTNLVWMHPNDAKKRGLRHGDKVKVISRRGEYVSNLDTRGRNKCPEGLIYSTF
                     FDAGQLINKVTLDATDPISFETDFKKCAVKVVKA"
     gene            8025..8930
                     /gene="napG"
                     /locus_tag="PXD66_000653"
     CDS             8025..8930
                     /gene="napG"
                     /locus_tag="PXD66_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HDL6318981.1"
                     /translation="MKMDPNRRQFLKNATRTAAGVCGVGMILGLQQQQSFARQGVALR
                     PPGALPEQDFLSACTRCGQCVQACPYDMLHLASLISPVEAGTPYFIARDKPCEMCLDI
                     PCMNACPTGALSEELKDIDDARMGLAVLLDHETCLNWQGLRCDVCYRVCPLIDKAITL
                     EKVHNDRTSIHAKLIPTVHSDACTGCGKCEQACVLEEAAIKVLPMDLAKGLLGRHYRL
                     GWQEKQNAGKSLIEDVHPEGLRPAYDARMPEGLHEPVYQHMEVKPDVKVATPSRATQN
                     YVPNSTTVEAPEHFPDLDLNLKGVK"
     gene            8930..9808
                     /gene="napH"
                     /locus_tag="PXD66_000654"
     CDS             8930..9808
                     /gene="napH"
                     /locus_tag="PXD66_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HDL6318982.1"
                     /translation="MAANSPKNAGLEARQKLGWWHAYRFLILRRLSQLSIILMFLSGP
                     LWNVWILKGNYSGSLLLDTVPMTDPLMTAEVLATGYRPEWTVLVGALIMVAFYGIVGS
                     KLFCSWVCPMNMVTDTAAWLRRKLGIRQTAKIPRNLRYVILAVILVGSAISGTLLWEW
                     INPVAALGRVFVFGLGATLWLVLVVFLFDLLVAEHGWCGHLCPIGATYALIGAKSLIK
                     VKVVDRNRCDNCMDCYNVCPEPQVLRVPLHGKPEESTVILSKDCITCGRCIDVCAENV
                     FAFGMRFQGSIDVKNI"
     gene            9854..10282
                     /locus_tag="PXD66_000655"
     CDS             9854..10282
                     /locus_tag="PXD66_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HDL6318983.1"
                     /translation="MRKYLAFILAVMSGFAMAEAPKLSGSIDETTESVAPGFHNPQKD
                     VGTIPTTFPFQPPLVPHSIRGLQVSKNVNHCLGCHAIEPSKTTGATRLPPSHFTDRDG
                     KVYENESPRRYFCLQCHVQQTDVNPIIQNKFESVRESQAK"
     gene            10293..10901
                     /locus_tag="PXD66_000656"
     CDS             10293..10901
                     /locus_tag="PXD66_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HDL6318984.1"
                     /translation="MLRRFCNWLRSPSKMAIGAVILISALGGIFAWSGFNAGLAMTNT
                     EEFCSDCHMNDVVPEYRASAHYSNRSGVKAICSDCHVPHEFFPKWQRKIIAAKEVYAH
                     FTGKVDTKEKFEAHRGEMAEREWARMKANSSQECRNCHNFDDMDFTQQKNIAQQMHAL
                     AQEQGKTCIDCHKGIAHNLPHMEAIQKNFIPDDLLKKAEEKK"
     gene            complement(10963..11805)
                     /gene="cpdA"
                     /locus_tag="PXD66_000657"
     CDS             complement(10963..11805)
                     /gene="cpdA"
                     /locus_tag="PXD66_000657"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HDL6318985.1"
                     /translation="MDAKRMHEYIKLPVTGVIKLLQITDPHLFSNPEETLLNVKTVKS
                     FSAVIEQINKQAKQYFDLVLATGDLIQDNNIAGYHYFAQITNSLNSPIVWLEGNHDVQ
                     PSMSEILAQYKHILPHKQILLGEQWQLLMLNTQVVGMPYGELSYSQLKWLEEKLAQYP
                     QRFTLVALHHNILPTNSAWLDQHSLKNSNELSEIFAKFKNVKAIVHGHIHQQVDDIWH
                     GIPIYATPSTCIQFKPNCDQFTLDLQPQGWREIELHENGTIETVVKRLNSNDFLPNVN
                     AIGY"
     gene            12095..12229
                     /locus_tag="PXD66_000658"
     CDS             12095..12229
                     /locus_tag="PXD66_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/trimethylamine N-oxide reductase
                     NapE/TorE"
                     /protein_id="HDL6318986.1"
                     /translation="MRSELLKTLLLSGLILPLLMAFAVGAYGFIVWMLQLFVIGLPGA
                     "
     gene            12233..13396
                     /gene="torC"
                     /locus_tag="PXD66_000659"
     CDS             12233..13396
                     /gene="torC"
                     /locus_tag="PXD66_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HDL6318987.1"
                     /translation="MLGSIKALFTRPATKIGLGVLVGIGFIAGAISWHQFNKAMDHTS
                     TEEFCVSCHSMQQPLEELKKTAHWSNKSGVTATCSSCHLPHDYTEKYARKVQASREAW
                     AELSGKYKEEGSFEKHRLEMAEREWARFAANGSKECKACHSYERMNFDKMGEAARKAM
                     KPAAEKDQSCMDCHKGIAHHLPKADENALKSKFDQFVNKSPNQGKSYYTKGNAELFLD
                     EGLSQAIGQLETAVPVAFVQAGKNADLVELTMWRKEKGFGRIWYHDFGKNITDAVLTK
                     EFMANQPKFEVLETKEDPITGLNWQKVKLQAWIGKAQLLDDVTPIWANAENQYKTQCS
                     TCHRQPDVAHFDSNSWIGLFNGMVGFTNMDKQTGKEVLRYLQMHASDSEEAKH"
     gene            13496..15961
                     /gene="torA"
                     /locus_tag="PXD66_000660"
     CDS             13496..15961
                     /gene="torA"
                     /locus_tag="PXD66_000660"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HDL6318988.1"
                     /translation="MQQSRRQFLKNMSVMAAAVTMPNFLVPRNVFASEDLSQWKVSGS
                     HWGAMRAKIENGRVAEIKPFEFDKHPTEMLNGIKGLIYSDSRIRYPMVRLDWLKNRHN
                     SNTAQRGDNRFVRVTWDEALDLFYQELERVQKEYGPWALHTANVGWRSTGQFHSCGNH
                     MIRAIAMHGNSVGTAGDYSTGAGQTILPYVLGSTEVYSQGTSWEIILKESENIIFWAS
                     DPVKNLQVGWNCETHEAYAYLEKLKEKVAAKGVNVICVDPVKSKTQNFLGCEQQYINP
                     QTDVPFMLALAHTLYTENLYDKKFLDMYTVGFEKFLPYLLGETEDKVAKTPEWAAEIC
                     GISADKIREFARMLAGKRTQFIFGWAIQRQQHGEQPYWMGAVLASMLGQIGLEGGGIS
                     YAHHYSSIGIPESGAAMPGAFPLNIDEGQTPKYDNKDYKGYSDTIPVARMTDSLLHAG
                     ETIDYNGKKVTYAPFKMAIFTGCNQWHRHSERNKMKQAFQKLETIVSINYSWTATCRF
                     SDIVLPACTPFERNDIDAYGSYSNRGVIAMHKLVDPLYDSRSDFEIFRDLCRRFGKEK
                     EYSRGMDEMQWVEKLYKDCRRENRGKFEMPEFAEFWQKGYVLFPEGKPWVRHADFRED
                     PELHALGTPSGFIEIYSNKIASFGYDDCKGHPMWFEKAERSHGGKNSDKFPYWLQSVH
                     PDKRLHSQLCESKELRETYSVQGREPLYINPQDAAKHGIQDGDLVRVFNDRGQAIVGA
                     VLSDNFPTGVVRLQEGAWYSPLDESVGAIDTYGDPNTMTLDIGTSKLAQAVSANTCLV
                     NIEKFIGNAPAPNGFNGAIEVTA"
     gene            15961..16551
                     /gene="torD"
                     /locus_tag="PXD66_000661"
     CDS             15961..16551
                     /gene="torD"
                     /locus_tag="PXD66_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HDL6318989.1"
                     /translation="MTLAISREERQFIYGWFNAMLARELSDEQLNALQAGEFDDFFAY
                     LSELGFAEQVAKFQAEVTACRQLEFPRLELAADFAQLFLLEGNLSAIPYASAYLSGEA
                     LAKNLAEMDRLLGEFGLQVNRETKEPSDHLCVYLTLFDRLLAEKPLETQQQFLQNQLM
                     SWLPAWFAKAKTVKTNTGVYPALLRLFEQVLLKEQR"
BASE COUNT         4841 a         3433 c         3923 g         4435 t
ORIGIN      
        1 aagtgaaagg catttattgc tagataaaag cttccctttt caataaaaaa gagtatcata
       61 cgctctctta aacgtaaccc tacatttaag tctgtttcca acctatccca atttctttat
      121 tccttagggg catatagggt aaaaacaaaa aagctgaaac gcttttttta ataagtagcg
      181 agtcaaatgc cccatggcat ctattctttt aggaatagaa agcttaggct ttccgcaagg
      241 cacccgactt atttttttca cctcttgtag aaggtttatt ttcaatgaca attaaaactc
      301 ctattcaagc cgttcttgct tcaaaccaat attttttaga tcgccaagat gcaatggaat
      361 caaacgtgcg tagctaccca cgtaaattac cttttgctta ccaaaaagca caaggcgttt
      421 gggtaactga tgttgaaggc aatgaatacc tcgatttcct agcaggtgca ggaacgcttg
      481 cattaggtca taaccaccct aaattaatcc aagcagttaa agatgtatta gacagtggtt
      541 tacctttaca ctcgttagat attacgactc cgttaaaaga tgcttttacg gaagaattac
      601 tctctttctt tccaaaagat aaatatatcc tccaatttac tggcccgtct ggtgcagatg
      661 ctaacgaagc ggcaattaaa ttagccaaaa cctacactgg tcgtggcaac gtaattgctt
      721 tctccggtgg tttccacgga atgacccatg gtgcgttatc gctcaccggt aatttaagtg
      781 cgaaaaatgc agtacaaggc ttaatgccgg gcgtacagtt tatgccttat ccgcacgaat
      841 accgctgtcc gtttggcatt ggtggcgaag ccggtgcaaa agcagttgaa cattattttg
      901 agaactttat tgaagatgtt gaaagtggtg tggtaaaacc ggcagcggtg atccttgaag
      961 ccattcaagg tgaaggcggt gtggttccgg ctccaatcag cttcttacaa aaagtgcgtg
     1021 aagtcactca aaaacacggc atcttaatga ttgtggacga agttcaagct ggtttctgcc
     1081 gttcagggaa aatgtttgcc tttgaacacg caggcattga gccggatatc gtagtaatgt
     1141 caaaagcgat tggtggtagc ttaccgcttg cggtattagc gatcaaaaaa gagtttgacg
     1201 cttggcaacc ggcaggtcat accggtacat tccgtggtaa ccaattagca atggcaaccg
     1261 gctatgtatc gcttaaaatt atgcgtgaag agaatttagc gaaaaacgca caagagcgtg
     1321 gcgaatactt aactaacgca ttacgtgaat taagcaaaga attaccttgt attggcaatg
     1381 tgcgtggtaa aggcttaatg atgggaattg atattgttga tgaacgtcag ccaaaagatg
     1441 caacaggtgc atacccgcgt gattgcgatt tagccgttgc aattcaaaaa tactgcttta
     1501 aaaacaaatt gttattagag cgtggcggac gtggcggtaa tgtggtgcgt gttctttgtg
     1561 ccgtgaatat tacccaagca gaatgtgaag agtttattaa acgctttaaa caatctgttg
     1621 ctgaagcagt gaaagcggtg cgtggttaat agttagaatg gtaggagcga aacatttttc
     1681 gccctaatgt aaaaaggggc gaatatttat tcgcccctac accaatagga tagaaataat
     1741 gtcaaaccta gaaaaacaca gacaatccct gttttgtagc gatccaaaat cgattgcgga
     1801 ttatgaatcg gcaatgaata acgctgtgaa agcggtttca gactggctga aagaagaaaa
     1861 aatgtacacc ggtggctcaa ttaaagagtt gcgtgccaaa atcaacggtt ttaacccaac
     1921 ccaagctggt gtgggcgtgc aaaaatcact ggagcatttg gtggaaattt tcttaaagcc
     1981 aagcctcaaa gttcaccacc ctcattcttt ggcccactta cattgcccga caatggtaac
     2041 cagccaaatt gcagaagtgt taattaatgc caccaaccaa tcaatggact cttgggatca
     2101 aagcccggca ggttcgatta tggaagagca tttaattgac tggctacgtc aaaaagtcgg
     2161 ctatggcaaa ggaacttcag gagtctttac ctcaggtggt acgcaatcaa acctgatggg
     2221 cgtattattg gctcgtgact gggcgatagc gaaccattgg aaaaaggcag acggttcaga
     2281 gtggtcggtg caagccgacg gtattcctgc cgatgcaatg caaaaagtga aagtggtttg
     2341 ttccgaaaac gctcacttct cggtgcagaa aaatatggca atgatgggaa tgggtttcca
     2401 atcggtagtg actgtaccgt caaatgccaa tgcacaaatg gacgttgaag ctcttgcgaa
     2461 aacgttggcg gatttaaaag ccgaaggcaa aattgtggct tgtatcgtcg caaccgcagg
     2521 gacgaccgat gccggtgcga ttgatgattt aaaagcgatc cgtaaattag ccgatgaaca
     2581 tcaggcttgg gttcacgttg atgctgcttg gggtggtgca ttattactgt cgaaagattt
     2641 ccgccacttc ttagacggca ttgaattaac cgattccatt acccttgatt tccacaagca
     2701 tttcttccaa tcaatttcgt gtggggcatt cttgctaaaa gatgaagcga attaccgctt
     2761 tattgattac aaggcagact acctgaattc agaatatgac gaagaacacg gcgtgccgaa
     2821 tttagtgtca aaatcattgc aaactacccg ccgttttgat gcgttaaaat tatggtttac
     2881 cgttgaagcc ttgggtgagg agctatacgc ttcaatgatc gaccacgggg tttatctttc
     2941 taaagaagtg gaagcctaca ttaatgccac cgatggcttg gaaatgttag tgccggcaca
     3001 atttgcctca gtcttattcc gtgtcgtacc acaaggttac ccggcagaat ttgtggatac
     3061 cttaaaccaa aatgtggcag atgagttatt tgctcgtggc gaagctaaca ttggtgtgac
     3121 caaagtcggt gagcatcaat cactgaaaat gactacttta agcccgattg caacgcttga
     3181 aaacgtaaga gcattattag ctcaggtgtt ggcagaagca gatcggatta aagattcgat
     3241 tgtaaatgga acttatgttc cgccaattga ttaaactgcg ttaaaaataa aaaggtgttt
     3301 tcgacattta tttgtgtttt aatgtttgca ttcggtagtc tttatcgaat gcaaattttt
     3361 ttaacaagga gtttttatga aaaaagtcct gggtttaaca ttgatggcaa tggcgttatc
     3421 ggcgtgtaat tcaactgctt ccaacggttc ttcacaagca aatacaggtg ttgcaccggc
     3481 gaatgtgatt agcgtgaaac aggcgttagc ggcagcagat gacagctacg tgacggtaga
     3541 aggcacactc ttaaacaaag tcggtgacga aacttactta tttaaagatg cgacagggca
     3601 aatccgtgtt gagattgacg atgaagtatg ggcaggtcag cacgttggta gcgaaggcaa
     3661 taaagtccgt ttatacggag agattgacaa aggtttcaat aaaaccgagt tagatgttaa
     3721 aacactcact gttctgtaga agatcaaacc ttaatttggt tccccgcctt gtttggcggg
     3781 gtttttacgt taattaatca aaccatcctt ttgcaaaaaa acgcaaaatt ataccgcttg
     3841 ttccttcaat attgtgctac aagatgctgt gatttgctgc aaaatagcgg tgtaatcagt
     3901 ctcttttaaa ccaatattac cgaaatagtg catttttaca tcacgaatcc cacagaactc
     3961 aaacagccct tttcccagcg tatgttcaaa tgctgctaaa aagccttttc gttcatattt
     4021 ttcattggaa ttaccgagcg ttacaaattg ttgcattttt ttgccggtca gcaagccgat
     4081 actttcaatt tccccgttac gataggcaaa atcataactt aacaccctgt ctaaatagcc
     4141 ttttaaaatt gccggaaagc ccatccacca aagcgggtaa attaacgtaa tcacatcagc
     4201 ttcaagccaa tattgatgct caattgccac atctgcttca tagtgtttac tgagtgtgcc
     4261 ttgaaattcc gcccagtcta atatcggctt gaagttgagt tggtagagat ctcgtacaac
     4321 caccttattt ggaacggaag ctgctatcgt tttttccaaa atcgcatggt taaagctgga
     4381 gggattcggg tgtgcgtaaa taatcaggtg attcatagcg ttttccttta ctgtgtcgag
     4441 gcagctacta gaagatttta gcgaaacttt ctttgatgaa aatcaatatt ttggcgaata
     4501 taccggcaaa taccccgttt gaggaatgaa cggttttaag tgaacattag ataatttagc
     4561 ctattatagt gatgagtaaa aaggtgtttt cttgactgat tatcctaatc aatcacttcc
     4621 tcgccgtagc tttttgcgag ggcaattttt agactcgtta aaaagccaaa aagtcagtca
     4681 gcaagggtat gagccgattc gtccgccttg ggctaattta gcaaattttt tagaaaaatg
     4741 caccgcttgc ggtaactgca ttcaggcttg cgaaacccag attattgtaa aaggtgctgc
     4801 gggctaccct gaaattgatt ttagccgtgg tgaatgcact ttttgtgaaa aatgtgtgca
     4861 ttcttgcgag gcagaggtat tccgagcagt ttcagaacag ccttggagcc ataaaattga
     4921 gattcaagcc ggctgtttat taaagctcgg aacggaatgc cgaagctgtg gcgatagctg
     4981 tgagatgaga gtgattcgtt tccgcccgag tttaggtggc attgccgaaa tggtattaaa
     5041 tttggaaagc tgtaatggct gtggggcttg tttgagcgtt tgtccgacaa gtgcgataaa
     5101 aattcagcca ttacaaacac aggcaaacag agatgagtga aaaattatcc gaacaggaaa
     5161 actggtatgt gtgcagtctc gtggtgcaag caaaaccgga aaaattggaa gaagtaaaag
     5221 ccgagattct gaagattcct cataccgaaa ttcacggtga gaaaacagat gaaggcaaat
     5281 tggtcgtgac gattgaaagc aatgtgcatt tagccctttc cgatcgaatc gatcaagtaa
     5341 gagatattaa aggcgtgatt gtggtgtctt taatttctaa ttacattgat gaacagtgat
     5401 taatgattat ttttgaagcc aaacgtggga catagttatg gaactcaatc gtagagattt
     5461 tatgaaagcg aacgccgctg tggcagctgc tgcagccgca ggaattatga ttcctgtgaa
     5521 aaacgttcag gcagatgaca ccggtattcg ttgggataaa gccccttgtc gttactgtgg
     5581 tacaggttgt agcgtgctag tcggtactaa agacggtaaa gtagtggcaa cccaaggtga
     5641 cccggatgca gaagtaaacc gtggtttaaa ctgtattaaa ggttatttct tatccaaaat
     5701 gatgtacggt gcagaccgta tgcaagagcc aatgttgcgt atgaaagacg gtaagttcga
     5761 caagaacggc gatttcacgc cggtttcttg ggatcaagcc tttagcatta tggcggagaa
     5821 gattaaaacc actattaaaa atgacggccc gaatgctgta ggtatgttct catcaggtca
     5881 aacaaccatt tttgaaggtt atgcgaaagt gaaactttgg aaaggtggtt tccgttcaaa
     5941 tacgattgac ccgaatgccc gccactgtat ggcgtccgct gcggttgcct ttatgcgtac
     6001 ctttggtatg gacgagccga tgggctgcta taacgacatc gaaaaaaccg atgcgttcgt
     6061 gctttggggt tcaaatatgg cagaaatgca cccgatttta tggtctcgta tttctgatcg
     6121 ccgtttatca gacaataaag tgaaagtggt ggtaatgtcc acttacgaac accgttcgtt
     6181 tgaattagcc gatacaccga ttatcttcaa accacactca gatttagcaa ttttaaacta
     6241 cattgcgaac tacattatcc aaaacgataa agtaaattgg gattttgtta acaaacacac
     6301 caaatttaaa cggggtgaaa ccaatatcgg ttacggttta cgtcctgaac acaaattaca
     6361 acaaaatacc aatgcgaaaa ctgccggtaa aatgtatgac agcgattttg aagagtttaa
     6421 gaaaatcgtt gccccttata cattagaaga agctcaccgt atttcaggcg tgccgaaaga
     6481 tcagcttgaa acattagcga aattatatgc agatcctaac caaaaattag tgtcgttctg
     6541 gacgatgggc tttaaccaac atacccgtgg tgtgtgggta aaccatatga tgtacaacgt
     6601 ccatttatta accggtaaga tcgcattaga aggttgtggg ccgttctcac tcacgggtca
     6661 gccgtccgct tgtggtactg cccgtgaagt gggtacattt attcaccgct taccggcaga
     6721 tatggtggtc acaaatccga aacaccgtga gattgtagag aattcttgga aattaccggc
     6781 tggagcaatt cctgatgtac cgggctttca cgctacagct caaagccgtg ccttaaaaga
     6841 cggcaaaatg aaattcttat ggcagatgtg taccaacaat atgcagggcg gcccgaatat
     6901 caatgaagag atcttcccgg gctggcgtaa tcctgaaacc tttattgtgg tttcagatcc
     6961 gtacccatct gtttcagcag tggctgctga cttaattctt ccaacctgta tgtgggtgga
     7021 aaaagaaggg gcttacggta acgcagaacg ccgtacccaa ttctggcgtc agcaagttaa
     7081 agggccggca aatgcccgtt cagatacttg gcagttagta gagttctcta aatacttcaa
     7141 agtagaagaa gtatggccgg aagagttatt agctaaagca ccggaattgc gtggtaaaac
     7201 tctatacgaa atcctttacc gcaacggcaa agtagatagc taccaagtgc caactaacat
     7261 tccgggctat atgaatgacg aagcagatca cttcggttac tacattcaaa aaggcttatt
     7321 tgaagagtat gcggaatttg gccgtggtca cggtcacgat ttagccgact ttgacaccta
     7381 ccacaaagta cgtggcttac gttggccggt ggtaaaagac gagaaaaccg gcgaatacaa
     7441 agaaacttta tggcgttacc gtgaaggcta tgacccatac gttaaagccg gtgaagaagt
     7501 ggcattctat ggcaatgcgg acaaaaaagc agtgatttta ggtgtgccat acgaaccacc
     7561 tgcggaagca ccggacgaag aatatgactt atggttctgt accggtcgtg tactagaaca
     7621 ttggcacacc ggtacgatga cccgccgtgt gccggagtta cataaagcat tcccaaccaa
     7681 cttggtttgg atgcacccga atgatgcgaa aaaacgaggc ttacgtcacg gtgataaagt
     7741 gaaagtgatt tcacgccgtg gtgaatatgt atcgaactta gatacccgtg gacgtaataa
     7801 atgtccggaa ggtttgattt actcgacttt cttcgatgcc ggtcagttaa tcaacaaagt
     7861 gacacttgat gcgactgatc caatttcatt tgaaaccgac ttcaaaaaat gtgcggttaa
     7921 agtggtgaaa gcttaattgt aggggcaaat tatttgcctc agacaagcgg tcaaatttgc
     7981 aaattttttt acaaaaacga ccgcttacct aagaggaacc cacaatgaaa atggatccaa
     8041 atcgtcgcca gtttttaaaa aatgcaaccc gtaccgcagc cggtgtttgc ggtgtaggaa
     8101 tgattttagg gctacagcaa caacaaagtt ttgcccgtca aggggtagca ctacgcccac
     8161 cgggtgcatt gccggagcaa gatttcctga gtgcttgcac ccgttgcgga caatgtgtgc
     8221 aggcttgtcc ttacgatatg ttgcatttgg catcgcttat ttcaccagtg gaagccggca
     8281 cgccatactt tattgcacgg gataaacctt gcgaaatgtg cttggatatt ccctgtatga
     8341 atgcttgccc aaccggggca ttaagcgaag agctaaaaga cattgacgat gcacgaatgg
     8401 ggcttgcggt gttgctcgac cacgaaactt gtcttaactg gcaaggcttg cgttgtgacg
     8461 tgtgttaccg tgtttgtccg ttaatcgaca aagcgattac tcttgagaaa gtgcataacg
     8521 accgtacctc gattcacgcc aaactgattc cgaccgtgca ttctgatgct tgcaccggtt
     8581 gcggtaaatg tgaacaggct tgcgtattgg aagaggcagc gatcaaagtg ttaccgatgg
     8641 atttagccaa aggcttgctc ggtcgtcact atcgtttagg ctggcaagag aaacaaaatg
     8701 ccggtaaatc gctgattgaa gatgttcacc cagagggttt acgcccagct tatgatgcca
     8761 gaatgccgga aggcttacac gagccggtgt atcagcatat ggaagtaaaa cctgatgtga
     8821 aagtggctac gccaagtcgg gcgacacaaa actatgtgcc gaactctacg acggtggaag
     8881 ctccggagca tttccctgat ttagacttga atctcaaggg ggtgaaataa tggcagccaa
     8941 ttctccaaaa aatgcgggct tagaggctcg tcagaaacta ggttggtggc acgcttaccg
     9001 ctttttgatt ttaagacgct taagccaact cagcattatt ttgatgtttt taagcggccc
     9061 actttggaat gtctggattt taaaaggaaa ctacagcggt agcttattac tagataccgt
     9121 gccgatgaca gacccattaa tgaccgctga agtgctggca accggctatc gccctgaatg
     9181 gacggtattg gttggggcgt taattatggt ggcgttttac ggcattgtcg gcagcaagct
     9241 gttttgtagt tgggtctgcc cgatgaatat ggtaacagat actgctgctt ggttaagacg
     9301 caaactcggt attcgccaaa ctgctaaaat tccacgcaat ttacgctatg tgattttggc
     9361 ggtgatttta gtcggtagtg cgatttccgg aacactattg tgggagtgga tcaacccggt
     9421 tgcggctctc ggtcgggtgt ttgtattcgg tttaggggca acgctttggc tggttttagt
     9481 ggtgttcttg tttgacttat tggtggcaga acatggctgg tgtggtcatc tttgcccgat
     9541 tggtgcaacg tatgccttaa ttggggcgaa aagcctgatt aaagtcaaag tggtagatcg
     9601 taaccgttgc gataattgta tggattgtta caacgtttgt ccggaacctc aagtgttgcg
     9661 tgtgccgcta cacggtaaac cggaggaaag cacggttatc ttatctaaag attgcattac
     9721 ctgcggacgt tgtattgacg tttgtgccga aaatgtattt gccttcggaa tgcgttttca
     9781 aggcagtatt gatgtgaaaa atatttaatt tttatctttt tgaaaacaag aattttaatg
     9841 gagtgaacct caaatgagaa aatatctcgc cttcatttta gccgtgatgt ccggctttgc
     9901 aatggcggaa gcccctaagt tatccggatc aattgatgag acaaccgaaa gtgttgcacc
     9961 gggatttcat aacccacaaa aagatgtggg cacgattcca accactttcc ctttccaacc
    10021 accgcttgtt ccacatagta ttcgtggctt acaagtgagc aaaaacgtaa accattgttt
    10081 aggttgccac gctattgaac catctaaaac cacaggtgcg acccgtttac cgccaagcca
    10141 ttttaccgac cgtgatggca aggtgtacga aaacgaatct cctcgtcgtt atttctgttt
    10201 gcaatgccac gtgcaacaaa ctgatgtaaa cccgattatt caaaataaat ttgaatctgt
    10261 acgtgaatct caagctaaat aaggagtaag taatgttgag aagattttgt aactggttac
    10321 gtagcccaag caaaatggca attggtgctg taatccttat ttctgcatta ggtggtattt
    10381 tcgcttggag tggttttaat gcagggttag caatgacaaa tactgaagag ttttgttcag
    10441 attgccatat gaacgatgtt gtgccggaat accgtgcctc tgctcactat tcaaaccgca
    10501 gcggtgtgaa agcgatttgt tcagattgcc acgtaccgca cgaattcttt ccaaaatggc
    10561 aacgtaaaat catagccgca aaagaagttt atgcacactt taccggtaaa gtagatacca
    10621 aagagaaatt tgaagctcat cgtggcgaaa tggcagaacg tgaatgggca cgtatgaaag
    10681 cgaacagctc tcaagagtgc cgtaactgtc ataattttga tgatatggac ttcactcaac
    10741 aaaaaaacat tgcgcagcaa atgcacgcat tggcacaaga gcaaggtaaa acctgtattg
    10801 actgccacaa agggattgca cacaatctcc cacatatgga agcgatccag aaaaacttta
    10861 tccctgatga tttgctgaaa aaagcagaag agaagaaata acttaacgtg tttcaataga
    10921 aaaccctcaa tttccattga gggtttcttt ttagtagcca agttagtaac caatggcatt
    10981 gacatttggt aaaaaatcgt tggaatttaa ccgcttgacg accgtttcta tcgtgccgtt
    11041 ttcgtgcaat tcaatttcac gccagccttg tggttgtaag tctaaggtaa actggtcgca
    11101 attcggctta aattgaatgc aagtagatgg ggtggcataa atcggaatac catgccaaat
    11161 atcatcaacc tgctgatgaa tatgaccatg cacaatcgct tttacattct taaattttgc
    11221 gaaaatctcg gataattcat tgctgttttt taagctatgc tgatccagcc acgctgagtt
    11281 tgtcggcaaa atattgtggt gcagagcaac caaggtaaac cgttgcggat attgtgccaa
    11341 tttttcttct aaccatttaa gctgtgagta agagagttcg ccataaggca tacctaccac
    11401 ctgggtattt agcatcagaa gctgccattg ctcaccgagt aaaatttgtt tatggggaag
    11461 gatatgttta tattgcgcca aaatttctga catactcggc tgaacatcgt gattcccctc
    11521 tagccacact atcggcgagt ttagcgaatt agttatctgt gcaaaatagt gataaccagc
    11581 aatattgtta tcttgaatca aatcacctgt ggcaagaact agatcaaaat attgcttcgc
    11641 ttgtttgttt atttgctcaa taactgccga aaaacttttt actgttttga cattaagtag
    11701 agtttcctcc ggattggaga aaaggtgagg gtcagttatt tgtaataact taattacccc
    11761 tgttacgggt aacttaatat attcgtgcat tcgttttgca tccattcaaa ttctagtaat
    11821 ttttaactgg tgagattcta tcagtaagct aaaagtaaaa atgtggacta tgccgcattt
    11881 ttgaaaaaaa gtgagattaa tcaaaaaaaa tttattattt tcgatagcta agacaatggt
    11941 aagtttgatt aatactttca tattgtaata gatgattttt atactgtttg gctgtttcta
    12001 gaacaacttc tttatatttt tgattgatat caaggcaaat tgtttctgaa gataaaataa
    12061 ttaacactta ttagttaata aaaaaggagt tgaaatgcgt tcagaactat taaaaacgct
    12121 tttgctatca gggctgattt taccgctatt gatggcgttt gccgtaggag cttacggctt
    12181 tattgtgtgg atgttacaac tgtttgttat tggtttaccg ggagcgtaga aaatgttagg
    12241 ttcaatcaaa gcattattta ctcggcctgc aaccaaaatc gggttgggag tgttagttgg
    12301 tatcggtttt attgccggag caattagctg gcatcaattt aataaagcga tggatcatac
    12361 cagcactgaa gagttttgcg tgagttgcca ctcaatgcag cagccgttag aggagcttaa
    12421 aaaaaccgcc cactggtcga ataaaagcgg ggtaacggca acctgttcaa gctgccactt
    12481 accgcacgat tataccgaaa aatatgcccg caaagtgcag gcaagccgtg aggcgtgggc
    12541 agaattatcc ggcaaatata aagaagaagg ctcatttgaa aaacaccgct tagaaatggc
    12601 agagcgtgag tgggcaagat ttgccgccaa tggttcaaaa gagtgtaagg cttgccacag
    12661 ctatgagcga atgaattttg ataaaatggg cgaagcagct cgtaaggcaa tgaaaccggc
    12721 tgcggagaaa gatcaaagtt gtatggattg ccacaaaggc attgctcacc acttaccgaa
    12781 agccgatgaa aatgcactga aatcgaagtt tgatcagttt gtgaataaat ccccaaacca
    12841 aggcaaatct tactacacca aagggaatgc cgaattattt ctagacgaag gtttaagcca
    12901 agctatcggg caattagaaa ccgccgtgcc ggtggcattt gtgcaagcgg gcaaaaatgc
    12961 ggatttggtt gagctgacaa tgtggcgtaa agagaaaggt ttcggtcgca tttggtatca
    13021 cgatttcggg aaaaacatta ccgatgcggt tctaaccaaa gagtttatgg caaatcagcc
    13081 gaaatttgag gtgttggaaa ccaaagaaga tccgattacc ggcttgaatt ggcaaaaagt
    13141 gaaactacaa gcgtggattg gcaaagctca attattagac gatgttaccc caatctgggc
    13201 gaatgcagaa aaccaataca aaacccaatg tagcacttgc cacagacagc ctgatgtcgc
    13261 ccacttcgat tccaacagct ggatcggctt attcaacgga atggtcggct ttaccaatat
    13321 ggataaacag accggcaaag aggtgctacg ttatttacaa atgcacgcct ccgatagtga
    13381 agaagcgaag cattaataat attactcccc tctttactaa agaggggaat taatcgagcg
    13441 ttgaataaca ttctctgtac tagctcacgt ttttacaaat aaagaaggac aagatatgca
    13501 acaatctcgc cgtcaatttt taaaaaatat gtctgtgatg gctgccgctg tcacaatgcc
    13561 gaatttcctc gtgcctcgca atgtgtttgc gagtgaagac ctcagtcaat ggaaagtttc
    13621 cggctctcac tggggggcaa tgcgtgccaa aattgagaac ggacgagtgg cagaaatcaa
    13681 accgttcgag tttgataaac acccgaccga aatgctcaac ggcattaaag gcttaattta
    13741 cagcgattcc cgtatccgct acccgatggt gcgtttagat tggctcaaaa accgccataa
    13801 cagcaacacc gcacagcgtg gcgataaccg ctttgtgcgg gtaacgtggg acgaagccct
    13861 cgacctgttc taccaagagt tagaacgggt acaaaaagag tacggcccgt gggcgttaca
    13921 caccgccaat gtgggctggc gttcaaccgg tcaattccac agttgcggta accatatgat
    13981 ccgagcgatt gcgatgcacg gtaacagtgt cggcacggca ggcgactatt ctaccggtgc
    14041 agggcagacc attctgccgt atgtgctggg ttcaaccgaa gtctattcgc agggcacgtc
    14101 ttgggaaatc atcttaaaag agagcgaaaa catcattttc tgggcaagcg acccggtgaa
    14161 gaacctgcaa gtgggttgga actgcgaaac ccacgaagcc tatgcctact tggaaaaact
    14221 gaaagagaaa gtggcagcaa aaggcgtgaa tgtgatctgt gttgatccgg tgaaaagcaa
    14281 aacccagaat ttcttaggtt gcgaacagca gtacatcaat ccacaaaccg atgtgccgtt
    14341 tatgttggca cttgcccaca cgctctacac cgagaacctg tacgacaaaa aattcttgga
    14401 tatgtacacg gtcggctttg aaaaattcct gccgtatctg ttaggcgaaa ccgaagacaa
    14461 agtggcgaaa accccggaat gggcggctga aatttgtggt atttccgccg acaaaatccg
    14521 tgaatttgcc cgaatgcttg ccggcaaacg cacccaattt attttcggct gggcaattca
    14581 acgccaacaa cacggcgaac aaccgtactg gatgggagcg gtgctagcct caatgttagg
    14641 gcaaatcggc ttggaaggcg gcggcatcag ctatgctcac cattacagct ctatcggtat
    14701 tcctgaatcc ggtgcggcaa tgccgggggc gttcccgctc aatattgatg aggggcaaac
    14761 cccgaaatac gacaacaaag attacaaagg ctacagcgac accattccgg tggctcgtat
    14821 gaccgacagc ttgctccacg ccggcgaaac cattgattac aacggcaaaa aagtgactta
    14881 cgctccgttc aaaatggcga ttttcaccgg ctgtaaccaa tggcaccgcc attccgagcg
    14941 gaacaaaatg aaacaggcgt tccaaaaact ggaaaccatt gtttcgatca actacagttg
    15001 gacggcaacc tgccgcttct ccgacatcgt gctgccggcc tgtaccccat ttgaacgcaa
    15061 cgatattgac gcctacggct catacagcaa ccgtggcgtg attgcaatgc acaagctggt
    15121 tgatccactc tacgactctc gctccgactt tgagattttc cgagatctct gccgccgttt
    15181 cggtaaagag aaagagtaca gccgtggaat ggacgaaatg cagtgggtag aaaaactcta
    15241 caaagattgt cgccgtgaaa acagaggtaa atttgaaatg ccggagtttg ctgaattttg
    15301 gcaaaaaggc tatgtgctgt tcccagaggg caaaccttgg gttcgccacg ccgatttccg
    15361 tgaagatccg gaattgcacg cacttggcac accgtccggc tttatcgaaa tttacagcaa
    15421 caaaatcgcc tctttcggct atgacgattg caaaggtcac ccaatgtggt ttgagaaagc
    15481 cgaacgttca cacggcggta aaaattccga caaattcccg tactggttgc aatcagttca
    15541 cccggacaaa cgcttacact ctcaactttg tgaatcgaaa gaactgcgtg aaacttacag
    15601 cgtgcaaggg cgtgaaccgc tttacattaa cccacaagat gccgccaaac acggcattca
    15661 agacggagat ttagtgcgag tgtttaacga ccgtgggcaa gcgattgtgg gggcggtgct
    15721 ttccgacaac ttcccgaccg gcgtagtgcg tttacaagaa ggggcttggt attcacccct
    15781 tgacgaaagc gtgggggcga ttgacaccta tggcgacccg aacacaatga ccctcgacat
    15841 cggcacgtcc aagttggcac aagcggtttc cgccaacact tgcttggtga acatcgagaa
    15901 atttatcggc aatgccccgg ctccgaatgg ctttaacggt gcgattgagg taacggctta
    15961 atgacgttgg caatcagtcg tgaagaacgg caatttatct atggctggtt taacgcaatg
    16021 ctcgcccgag aactttcgga cgagcagtta aatgccttac aagcgggcga atttgacgat
    16081 ttttttgcat acctcagcga attgggtttt gccgaacagg ttgcaaaatt tcaagcagaa
    16141 gtgaccgctt gtaggcagct ggaatttcct cgtctagaac tggcggctga ctttgcccaa
    16201 ctgtttttat tggaaggcaa tctcagtgcc attccttacg cctccgccta tttaagcggc
    16261 gaagcgttag cgaaaaattt ggcggaaatg gacagactgc tgggcgaatt tgggctgcaa
    16321 gttaatcgag aaaccaaaga gccgagcgat cacctttgcg tttatctcac cctttttgac
    16381 cgcttgcttg ctgaaaaacc gttggaaacg caacagcaat ttctgcaaaa ccaactgatg
    16441 agctggctcc ctgcgtggtt cgccaaagcc aaaaccgtca aaactaacac cggcgtttat
    16501 ccggcgttgc taaggttgtt tgaacaggtg ttgctgaagg aacaaagata aagttattta
    16561 tttgagaatg atttgtattg agcgaattga gaaaggaagt taaaatttca acttcctttt
    16621 ttattggctg ga
//
LOCUS       DANLXF010000034        16626 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.034, whole genome shotgun sequence.
ACCESSION   DANLXF010000034 DANLXF010000000
VERSION     DANLXF010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16626
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.034"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(214..729)
                     /locus_tag="PXD66_000662"
     CDS             complement(214..729)
                     /locus_tag="PXD66_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL6318990.1"
                     /translation="MAGINKVIIVGNLGNDPEMRTMPNGEAVANISVATSESWTDKNT
                     GARREVTEWHRIVFYRRQAEVCGQYLRKGSQVYVEGRLKTRKWQDQNGQDRYTTEIQG
                     DVLQMLGSRQGNDMGGQQGGWGNSAPAGNSYNQGNSNYGYNQTQSAAPQSKPAPQPEP
                     AMNNFDDDIPF"
     gene            884..3712
                     /gene="uvrA"
                     /locus_tag="PXD66_000663"
     CDS             884..3712
                     /gene="uvrA"
                     /locus_tag="PXD66_000663"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDL6318991.1"
                     /translation="MQHIDIRGARTHNLKNINLTLPRDKFIVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTVT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTISQMVDRVLEEPEGKRLMLLAPVVKERKG
                     EHVKLLENLTASGYLRARIDGEICDLSDPPALELQKKHTIEVVVDRFKVRADLATRLA
                     ESFETALDLSGSTAIIADMDDPNAEEIVFSSSFACPHCGYSLHELEPRLFSFNNPAGA
                     CPTCDGLGVEQFFDAKKVVQNEEVSLAAGAIKGWDRRSFYYFGLLKAVAEHYGFDLET
                     PFGQLPKKYQNIILNGTKEEIEFVYVNDRGDKVKRKHAFEGVLNNMARRYKETESNSV
                     REELAKYINNRPCMDCEGSRLRREARYVFLDKTNLPMVSEKSIGEALTFFEELKLAGQ
                     KEKIAEKILKEIRERLQFLVNVGLNYLSLSRSAETLSGGEAQRIRLASQIGAGLVGVM
                     YVLDEPSIGLHQRDNERLLNTLVHLRNLGNTVIVVEHDEDAIRAADHIVDIGPGAGVH
                     GGQVIAQGTAAEIMQIEESITGQFLSGKQKIEIPAQRVPYEPTKLLTLSGASGNNLKK
                     VKLEIPVGLFTCITGVSGSGKSTLVNDTLFPLAQNALNRAENSDVAPYESIDGLSHFD
                     KVIDIDQSPIGRTPRSNPATYTGLFTPIREIFAGTQEARARGYNVGRFSFNVRGGRCE
                     ACQGDGVIKVEMHFLPDVYVPCDHCKGKRYNRETLEIRYKGKTIHQVLEMTVEEAREF
                     FAPMPMIARKLQTLMDVGLSYIRLGQSSTTLSGGEAQRVKLATELSKRDTGKTLYILD
                     EPTTGLHFADIKQLLGVLHRLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGEI
                     IATGTPEEVAKDKRSHTARFLKEILAKG"
     gene            complement(3804..5537)
                     /gene="dauA"
                     /locus_tag="PXD66_000664"
     CDS             complement(3804..5537)
                     /gene="dauA"
                     /locus_tag="PXD66_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HDL6318992.1"
                     /translation="MLNKWFLTKNAFLAVKPFSALKDALNAGYGKQHLIRDIISGLTV
                     GVIAIPLSMALAIASGVPPQHGLYTAIVAGIVIALTGGSRFNISGPTAAFVVILYPVT
                     QQFGLSGLLMATMLSGGILVLMALLRLGRLIEYIPLPVTLGFTCGIGIVIATLQIKDF
                     LGLSIEKMPEHYLGKLEAIFSALPTINWADTLVGVVTLLVLTRWHKLRLPIPGHLPAV
                     FIGTLLSLLLIHFGQNVATIGSAFHYTLSDGTTGYGIPSVLPEFVLPWNLPNAEGNRI
                     DWNLTTIQALLPAAFSMAMLGAIESLLCAVVCDNMTDTKHHSNNELLAQGLGNIASPF
                     LGGITATAAIARSAANVRSGAVSPISSVVHALLVLFALLFLAKALSYLPLSSMAALLL
                     VVAWNMADIPQIIQLTRRSGKNEIAVLLTCITLTVLFDMVIAITVGVLLASLLFIRTI
                     AEMTKSFEMAHPTDLNDVLVYRISGPLFFAAADNLFADLHNKTVHTDHQIKHIVLQCD
                     AVTVLDAGGIHALTRFVQHMLPHQQLHLCNVQFQPMRMLVRSQKTVNEIPQIQFSSDL
                     ESAFDKIRQAN"
     gene            5779..6711
                     /locus_tag="PXD66_000665"
     CDS             5779..6711
                     /locus_tag="PXD66_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL6318993.1"
                     /translation="MDSLIHFLSAFLTNITWIGALELGLIYSLVALGVLISYKILDFP
                     DLTADGSFPLGGAVCVISILNQVDPWLATLYGTLAGAVAGMVTASLHIGFKIEKLLAS
                     ILMMIALYSINLRIMGMPNIAILGETTIYDVMPIESDLQLALVRLVMALLVVIIVKGL
                     FDLFFATEIGLAVRATGTNSRMAKAQGIAINKMTILGMAISNALIALGGALYVQSNGG
                     VDISIGVGTIVIGLAAVIIGEAIFSVKRIIWLTFAVIIGSILYRFFIALALNNETLNG
                     IGFGPQDLNLITALLVVIVLAVPRIKHKLLGKRG"
     gene            6713..7510
                     /locus_tag="PXD66_000666"
     CDS             6713..7510
                     /locus_tag="PXD66_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL6318994.1"
                     /translation="MIELNNLFITFNKGTAIENPVLRGLSLKVEQGEFVSVIGSNGAG
                     KSTLLNAISGDCAVDSGEILIQGNKVNQSTTWQRANQVARVFQDPMAGTCESLTIEEN
                     MALAYQRGRKRGLGFAIKRQMRELFKDKLALLGLGLENRLTDQMGRLSGGQRQAVSLL
                     MASLQPSNILLLDEHTAALDPKTTDFVLELTNKIVREQKLTTLMVTHSMRQALDYGDR
                     TVMLHQGQVAFDVSGEQRQKMDVPDLLQLFQQSRHEQLSDDGLLLGN"
     gene            7597..7818
                     /locus_tag="PXD66_000667"
     CDS             7597..7818
                     /locus_tag="PXD66_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6318995.1"
                     /translation="MLSIFKTPVEKETLDDWAKISVDVAKVAILAIPVILFGKEPTLI
                     KLTNLALLVLSIYVFLTLGRKLRQLKEVL"
     gene            8001..9926
                     /locus_tag="PXD66_000668"
     CDS             8001..9926
                     /locus_tag="PXD66_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDL6318996.1"
                     /translation="MTKYDDKIQHAFSENGLLSQNISGFRPRQAQLEMAQVVAKAVKF
                     ATPVVVEAGTGTGKTFAYLVPALLSGKKTILSTGSKNLQDQLFNRDLPTIQKALKYKG
                     KIALLKGRANYLCLERLDQVTAMGVLGDKSVLTDLKKVSKWQNATKTGDLSECITIAE
                     DSPILPQLVSTTESCLGSDCPHFKDCYVVQARRKAMEADVVVVNHHLFCADMAVKKTG
                     FGELIPEAELVIFDEAHQLPDIASQYFGQSLSSRQLFDICKDTNIVYRTELKDLSQLG
                     KAADHLTKVVQDFRLLMGGEGQIRGNLRELFRDKKVVDGLVKVAENIDFLSEVAKKSL
                     GRSETLDKIFERLAEVKVQLKKLMDTSVTGYCYWYEANGRSFGLHITPLMVADKFGEQ
                     LKNQQIGWVFTSATLEVGGKFDHFCQRLGIENAEQLVLQSPFDYQHQSLLCVPRYLPD
                     TNKSNTLTALGQLLKPVIEANKGRCFLLCTSYYMMRGLADFLREHSSLNVLLQGETSK
                     SRLLEKFVKEDHSVLVATQSFWEGIDVRGDDLSLVIIDKLPFTSPDEPLLKARMEDCK
                     IQGGDPFNDIQIPEAVITLKQGVGRLIRDVTDRGVVIICDSRLVMRNYGQTFLKSLPP
                     SRRTRDLGNVIQFLKQK"
     gene            10000..10365
                     /locus_tag="PXD66_000669"
     CDS             10000..10365
                     /locus_tag="PXD66_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDL6318997.1"
                     /translation="MLSTLLRMVASLALLFVMLYLGKLIVYLFPIGIPDSILGMLILF
                     GCLVIGIVKVEWVIPSAKPLTRYITLFFLPICVELIEHLELLRQNLNTFVLATALSTL
                     VSVVAIGVFAQWIFHYKRG"
     gene            10367..11053
                     /locus_tag="PXD66_000670"
     CDS             10367..11053
                     /locus_tag="PXD66_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDL6318998.1"
                     /translation="MSYLYTVLTVIMFIAGLKISQKLKSSFLNPFLIALALLIGVLLI
                     FNIPYQDYYQGNSPLNNLLGVSVVALAYPFYEQLPHIRKKWRQIVVILLFSTIITMIT
                     GLGFSLLFGASQEILAAILPKSVSMPMAIIITNEINGNVAIAAVGVMIAGLVGSILGF
                     TLLRLAKVRNVMVIGLSMGAASHALGTARCLEHSMKAGSYSSIALVACGVLSSIFAPL
                     IFNVIVTLCY"
     gene            11137..12900
                     /gene="msbA"
                     /locus_tag="PXD66_000671"
     CDS             11137..12900
                     /gene="msbA"
                     /locus_tag="PXD66_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HDL6318999.1"
                     /translation="MQEQITQEKDLSTLKTFKRLWPTISPFKAGLITGAVALVLNALV
                     DSSLIYLLKPLLDEGFGQANNDFLKLMAVLVVVFILVRGITNYIASYCLAWVSGKVVM
                     TLRRHIFQHLMYMPVSYFDNNPTGRLLSRVTYDTEMVASSASHSLVTIVREGAYLLSL
                     FVVMIYTSWQLSIVLFVMAPIIGVLISFVSKYFRQLSRNIQSSMGELTVTTEQMLKGH
                     KVVLSFGGQEIEKARFDRVSNDMRRKGMKVVSADGISDGVVQLIASFALSAVLYVATF
                     PEVMSENLTAGSFTVVFSSMMAMLRPLKSLTNVNSQFQRGMAACQTLFEFLDLKTEKD
                     HGLISVAKAEGNVEFKKVSFSYEGKEEKALDNISFTIPKGKTVALVGRSGSGKSTIAN
                     LLTRFYDVTDGQILLDGINIQDYRLKNLREQCSVVSQQVHLFNDTIANNIAYAAIDKY
                     SREEIIAAAKAAHAMEFIEKLENGLDTVIGENGASLSGGQRQRLAIARALLRNSPVLV
                     LDEATSALDTESERAIQSALEVLQKDRTVLVIAHRLSTIEKADEILVVDHGNIIERGS
                     HTELLAQGGAYKQLHSLQFGA"
     gene            12989..13621
                     /gene="lolB"
                     /locus_tag="PXD66_000672"
     CDS             12989..13621
                     /gene="lolB"
                     /locus_tag="PXD66_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HDL6319000.1"
                     /translation="MKKLTKLLSLSALLVLTACNSVLNASTEVQQPTVQIPHDDPQWQ
                     QHLAKLSQIQGYTATGQFGYISPEERFSSHFNWRYTNPIQFGLELSSNLSTKSLKLER
                     NKRGLTVSDNEGNSRSDRDVDALMQEIIGVSFPIDQFAYWVKGLPEKDGNYIVNEKRQ
                     LSQFSYPLNGTLWKASYVEYHEDRVPNLPKLIVLENGTQTLKIRIEKWAY"
     gene            13609..14463
                     /gene="ispE"
                     /locus_tag="PXD66_000673"
     CDS             13609..14463
                     /gene="ispE"
                     /locus_tag="PXD66_000673"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDL6319001.1"
                     /translation="MGVLMKKVTLPSPAKLNLFLYITNKRADGYHELQTLFQFLDFGD
                     EIEIEITESSEVELINPVEGVATEENLIYRAAKLLQKTTACRQGCRIGIKKNLPMGGG
                     VGGGSSNAATVLVGLNHLWNTELSLEKLAELGLSLGADVPIFVRGMAAFAEGVGEVLT
                     PCDPQEKWYVVLKPEVSISTAAVFTDPNLPRNTPKRPLAELLSLPWENDCEKVVREKY
                     SAVEDLIQELLQYAQFRLTGTGACIFAEFETKAEAEQVFAHKPEAVFGFVAKGQNISP
                     LHQMLFQI"
     gene            14489..15439
                     /locus_tag="PXD66_000674"
     CDS             14489..15439
                     /locus_tag="PXD66_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HDL6319002.1"
                     /translation="MTDIKLFAGNATPELANRIAKHLYTSLGNATVGRFSDGEIQVQI
                     NENVRGGDIFIVQSTCAPTNDNLMELIVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVIADFLSSVGVDRVLTCDLHAEQIQGFFDVPVDNVFGSPVLIDDILKKS
                     DLVNPIVVSPDIGGVVRARAVAKLLNDTDMAIIDKRRPKANVSQVMHIIGDVADRDCI
                     LVDDMIDTGGTLVKAAEALKERGARRVFAYATHAVFSGTAAQNLANPALDEIVVTDTI
                     PLSNEIKALNKVRVLTLSGMLAEAIRRISNEESISAMFDA"
     gene            15517..16527
                     /gene="pyrD"
                     /locus_tag="PXD66_000675"
     CDS             15517..16527
                     /gene="pyrD"
                     /locus_tag="PXD66_000675"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HDL6319003.1"
                     /translation="MYSLIRKLLFALDTEKAHQFSIQALALAGKSPFSILPTPDNPTQ
                     VMGLTFKNPIGLAAGADKNGEAIDGFGKLGFGFIEVGTITPLAQDGNPKPRQFRIVDA
                     EGIINRNGFNNKGVDVLVENVKNATYSGILGINIGKNAVTPIEHSFDDYQICLRKVYQ
                     YADYVAVNISSPNTKNLRSLQYGEALDDLLRNLKNEQAQLSQKMGKYRPLVLKIAPDL
                     TDEEIVSVADSLIRYQIDGVIAGNTTLSRESIAGLPYSEQLGGLSGKPLHSLSTRLIS
                     KLSAELGERIPIIGCGGVHSLESGQEKVNAGASLLQVYSAMIYQGPSLIQTLAKHLQL
                     NS"
BASE COUNT         4796 a         3132 c         3843 g         4855 t
ORIGIN      
        1 tgagaaatgg ggcaataata aatctgtcaa taagttactt attaatcgaa tgaaaagtgg
       61 atttattaat tatattaatt aatagggaac acttattact tggaagtgtt ccctattatt
      121 ttgagtaagg taggggcgct ttatatgagt agctttttat ttcatcgaaa tgcggtgtat
      181 ggaacccatg agctacttaa gtcaatactt tgattagaac ggaatatcat catcaaaatt
      241 gttcattgcc ggctcaggct gtggtgccgg tttagattgc ggtgcagcac tttgggtttg
      301 gttgtaacca taattgctgt tgccttgatt gtagctatta cccgcaggag cagaatttcc
      361 ccaaccgcct tgctgaccgc ccatatcatt accttgacgg ctgccgagca tttgtagcac
      421 atcgccttga atttcggtgg tgtaacgatc ttgcccgttt tggtcttgcc atttacgggt
      481 ttttaaacgt ccttctacgt aaacttgaga gcctttacgt aaatattgcc cgcatacttc
      541 tgcttgacgg cggtagaata caatacgatg ccactcggtt acttcacggc gtgcaccggt
      601 gtttttatcc gtccagcttt ctgaggttgc cacactaata tttgcaaccg cttcgccatt
      661 tggcattgta cgcatttcag ggtcgttacc taaattaccg acaataatta ctttattgat
      721 accagccata aaatcctctg tttgataaat taaactaaaa tttgctaagt attctacctg
      781 aaaatccaaa agatgaacac caaaaaactg atattttgta cagttttatt aaaatatgcg
      841 ataatgatcg aaaattgaga aaatagcact ggacattaaa aatatgcaac atatcgatat
      901 tcgtggagca agaacacaca acttaaaaaa catcaattta accttgccga gagataaatt
      961 cattgtaatt accgggcttt ccggctcggg caaatcgtct ctggcgtttg atacgctcta
     1021 tgcggaagga caacgccgtt atgtagaatc actgtctgca tacgcccgcc agtttctctc
     1081 cttaatggaa aagcctgatg tggatcatat tgaagggctt tcccctgcga tttcgattga
     1141 gcaaaaatca acctctcaca acccacgttc gaccgtagga acggtaacgg aaattcacga
     1201 ctatttacgt ttattgtttg ctcgagtggg cgagcctcgt tgccctgacc acgatgtgcc
     1261 attggctgct caaactattt cgcagatggt ggatcgagta ttggaagaac cggaaggaaa
     1321 acggttgatg ctacttgccc ctgtggtgaa agagcgtaaa ggcgaacacg taaaattatt
     1381 ggaaaatttg accgctagtg gctatttgcg ggctagaatt gacggtgaaa tttgcgattt
     1441 atccgaccca ccggcattag aattacagaa aaaacacacc attgaagtgg tggtggatcg
     1501 ctttaaagta agggctgatc tagccacccg tttggcggaa tcttttgaaa cagcgttaga
     1561 tttatccggc tctaccgcaa ttattgcaga tatggacgac ccaaatgccg aagaaattgt
     1621 gttttcgtcc agctttgctt gtccgcattg tggttactct ttacacgaat tagaaccacg
     1681 ccttttctcg tttaacaacc cggcaggtgc ttgcccgact tgtgatggtt tgggcgttga
     1741 gcagtttttt gatgccaaaa aagtagtgca aaatgaagag gtttcattag ccgccggtgc
     1801 gatcaaaggc tgggatcgcc gcagtttcta ctattttggc ttattaaaag ccgttgccga
     1861 gcattatggc tttgatttag aaaccccatt cgggcagcta ccgaaaaaat atcaaaacat
     1921 tattttaaac ggcactaaag aagagattga gtttgtttat gtcaatgacc gaggcgataa
     1981 ggtcaaacgt aaacacgctt ttgaaggggt gctgaataat atggctcgcc gctataaaga
     2041 aaccgaatca aactcggtgc gtgaagagtt ggcgaagtat attaataatc gtccttgtat
     2101 ggattgcgaa ggctcacggt tacgccgtga agcccgttat gtattcttag ataaaaccaa
     2161 tttgccgatg gtttcggaaa aaagtatcgg cgaggcatta accttttttg aagagctgaa
     2221 attagccggt cagaaagaaa aaattgctga gaaaatcctc aaagaaattc gtgaacggct
     2281 acagttttta gtgaatgtcg gcttgaatta tctttctctt tcccgctcgg cagaaaccct
     2341 ttcaggtggc gaggctcagc ggattcgcct tgccagccaa attggtgcgg gtttagtagg
     2401 ggtaatgtat gtgctggatg agccttccat cggcttgcac caacgagaca atgaacgcct
     2461 actgaatact ttggtgcatt tacgcaattt aggcaatacc gtgattgtgg ttgaacacga
     2521 tgaagatgcg atccgagccg cagatcatat cgtggatata ggcccgggtg ccggtgtaca
     2581 cggtgggcaa gttatcgcac aaggtacggc agctgaaatt atgcaaattg aggaatctat
     2641 taccggacaa ttcctctcag gcaagcaaaa aattgagatt ccggctcagc gtgtgcctta
     2701 tgagccaact aaattgctca ccttatcggg agcaagtggt aacaacttga aaaaagtgaa
     2761 attagagatt ccggttggtt tatttacttg tatcacgggt gtatcaggtt cgggcaaatc
     2821 aactttagtt aatgacacac tattccctct ggcacagaat gcgttaaacc gagcggaaaa
     2881 cagcgatgtt gctccttatg aatcgattga cggcttatcg cattttgaca aggtaattga
     2941 tattgaccaa agcccgattg ggagaactcc tcgctcaaat ccggcaactt acaccggatt
     3001 atttacgcca attcgtgaga tttttgccgg cacgcaagaa gctcgagcca gagggtataa
     3061 cgtagggcgg tttagcttta atgtgcgggg cggacgttgc gaagcctgtc aaggtgacgg
     3121 cgtgattaaa gttgaaatgc acttcttgcc tgatgtgtat gtgccttgcg atcactgtaa
     3181 aggcaaacgc tataaccgtg aaaccttgga aattcgctat aaaggcaaaa ctattcacca
     3241 agtgttagag atgacggtgg aagaggcgag agaatttttt gcccctatgc cgatgattgc
     3301 ccgcaagttg caaaccttga tggacgtggg gttatcttat atccgtttag ggcaatcttc
     3361 tactacgctc tcaggcggtg aagcccaacg ggtgaaattg gcgaccgagc tttccaaacg
     3421 agacaccggc aaaacgctct atattttaga tgaaccgacc accggcttgc attttgccga
     3481 tattaaacag ttactcggcg tgctacatcg cctgcgtgat caaggcaata cgattgtggt
     3541 gatcgaacat aatttagatg tgattaaaac cgcagactgg attgtcgatc ttggccctga
     3601 aggtggcagt ggcggcggtg aaattattgc taccggcaca ccggaagaag tggcaaaaga
     3661 caaacgttct cacaccgcac gcttcttaaa agagattttg gctaaaggct aacgttcaac
     3721 acaagcggtc atattttcgg aaaatgttgc aatttgcaaa aagttggaaa atatgaccgc
     3781 ttgttttata tccggagtta ttcctagtta gcctgtctga ttttgtcgaa ggcagattca
     3841 agatcgctac tgaattgaat ttgcgggatt tcgtttaccg ttttttgtga gcggactagc
     3901 attctcatcg gctgaaattg cacattacag agatgaagtt gttgatgtgg caacatatgc
     3961 tgtacaaagc gggttagggc gtgaatgcca ccagcatcta atactgtaac ggcatcgcat
     4021 tgcagcacaa tatgtttaat ttggtgatcg gtatgtacgg ttttattgtg cagatcggca
     4081 aagagattat cggcggcagc aaaaaataac ggaccgctga tccgataaac caacacatcg
     4141 ttcaaatcgg ttgggtgagc catttcaaag gatttagtca tttctgcaat agtacggata
     4201 aatagcaggc ttgccagtaa cacccctacc gtaatggcaa ttaccatatc aaataatacc
     4261 gtgagggtaa tgcaagtcag taaaaccgca atttcatttt tacctgaacg gcgggtaagt
     4321 tgaatgattt gtggaatatc tgccatattc caagctacta ccaataatag tgcagccatt
     4381 gaggaaagag gcaaatagga aagtgcttta gccaaaaaga gtagggcaaa taaaaccaac
     4441 aaggcgtgaa ccacactaga aatgggggaa actgccccgg aacgaacatt ggcagccgat
     4501 ctcgcaatcg ctgctgtggc ggtaattccc cctaaaaatg gcgaggcaat attacctaag
     4561 ccttgggcta atagttcgtt gttagagtgg tgcttggtat ctgtcatatt atcgcacact
     4621 accgcacata ataacgattc gatagcccca agcattgcca ttgaaaaggc agccggtaat
     4681 aaggcttgaa tggtggttaa gttccaatct atccgattcc cctctgcatt cgggagattc
     4741 caaggtaaca caaattcagg taatacgctt gggataccat acccagtggt gccgtcagag
     4801 agcgtgtagt ggaatgctga gccgatagtg gcaacgtttt gtccgaaatg aatcagcagt
     4861 aatgataaca gcgtgccgat aaagacggcg ggtaaatgcc cgggaatagg caaccgtagc
     4921 ttgtgccaac gagttaaaac caacaatgtt acgacaccca ctaaggtgtc agcccaattt
     4981 atggttggca atgctgaaaa aatagcctct agcttgccaa gatagtgttc aggcattttt
     5041 tcaatgctta agcccagaaa atctttgatt tgcaaggtgg cgatcacaat accgatgcca
     5101 caggtaaaac ccagtgtgac aggtagagga atgtattcaa ttaagcgtcc taatcggagt
     5161 aacgccatca atactaaaat tcctccggaa agcattgttg ccatgagtaa tccacttaag
     5221 ccaaactgct gcgtgacggg gtatagaatc accacaaaag cggctgttgg gcctgaaata
     5281 ttaaatcttg aaccgcctgt tagggcaatc acaatcccgg caacaatcgc cgtatataaa
     5341 ccgtgctgtg gtggcacacc gctggcaatc gccaacgcca tagataacgg aatagcaata
     5401 acgccaacgg ttaagcctga aataatatct ctgattaaat gttgtttacc ataacctgca
     5461 ttgagagcat cttttaatgc actaaaaggc ttgacggcaa gaaaagcatt tttagttaaa
     5521 aaccatttat ttaacataga atgttcgagt gaagagaatg tttcgcttat tttactgcaa
     5581 tgaataaaat taaatttgag ctaacgcaag aaaaagcagg aaagacgaaa tatgttggag
     5641 acaagcggtt gttttaggcg aaaaatttgc aaatacaaat aattagctga ttttattttg
     5701 aaaatagcgt aaaatgccac tttaatttgt actactatag tagtgcattt gtgtgatttc
     5761 attaactata ataaacaaat ggacagtctt attcattttt tgagtgcctt tttaactaat
     5821 atcacttgga ttggggcgtt agagctgggg ttaatttatt cccttgttgc ccttggggta
     5881 ttaatttctt ataaaattct ggattttccg gatctgaccg cagacggcag tttcccttta
     5941 ggaggggcgg tgtgtgtgat cagcattttg aatcaagttg atccttggct ggcaacttta
     6001 tacggcacgc ttgccggagc agtagccggt atggttactg caagtttgca tatcggcttt
     6061 aaaatcgaaa aattgcttgc cagtatttta atgatgatcg cactctattc gattaatcta
     6121 cgcattatgg gaatgccgaa tattgctatt ttaggcgaaa ccacgattta cgatgtaatg
     6181 ccgattgaaa gcgatctaca gttagcctta gtacgattag tgatggcatt gctggttgtt
     6241 attatcgtaa aaggtttgtt tgacctcttt tttgcgaccg aaatcggttt ggctgtgcgg
     6301 gcaaccggta ctaatagtcg aatggcgaaa gcacagggta ttgcgattaa taaaatgacg
     6361 attttaggga tggcgatttc taatgcgtta attgccttag gtggtgcttt gtatgtgcaa
     6421 agcaacggtg gtgtagatat ttcgattggg gtaggaacta tcgtgattgg cttggcggcg
     6481 gtaattatcg gggaagcaat tttttcggta aaacggatta tttggcttac ctttgcggtg
     6541 attattggct caattcttta tcgtttcttc atcgcactag cattaaataa cgaaacttta
     6601 aacggcattg gctttggccc gcaagatctg aatttaatta cggcgttatt ggtggtgatt
     6661 gtattagcgg ttcctcgaat taaacacaaa ttgctcggta agcgaggtta atatgattga
     6721 actcaataac ttatttatta ccttcaacaa agggacagca attgagaacc ctgtgctgag
     6781 aggcttatcc ctaaaagttg agcaaggcga atttgtgtcg gtaattggta gcaatggggc
     6841 gggtaaatct accttattaa atgcgatcag tggtgattgt gcggtggact ccggtgagat
     6901 tttaattcaa ggaaataagg taaaccaatc caccacttgg caacgggcaa atcaagtcgc
     6961 ccgagttttc caagacccga tggctggcac ttgtgaaagt ttaacgattg aagaaaatat
     7021 ggcactggct taccaacgtg gacgtaaacg tgggttgggc tttgcgatca aacgccaaat
     7081 gcgagagtta ttcaaagaca aacttgcttt gttagggcta ggtttggaaa atcggcttac
     7141 tgatcaaatg gggcgtttat caggcgggca acgccaagcg gtgagcttgc tgatggcatc
     7201 acttcagccg tctaatatct tattgttaga tgaacacacc gcggcactgg atccgaaaac
     7261 caccgatttt gtgcttgaac ttaccaataa aattgtaaga gaacagaaat taaccacgct
     7321 aatggtaacg cattcaatgc ggcaagccct tgactatggc gatagaaccg tgatgttaca
     7381 ccaagggcaa gtggcgtttg atgtttcggg cgagcaacgg caaaaaatgg acgtgccgga
     7441 tctgttacaa cttttccaac aaagtcgcca cgagcagttg agtgacgatg gattattact
     7501 cggcaattaa ttttgcgatc tgcatcgaaa aaatgaattt ctccttttgt ttttggctgt
     7561 caaattttaa ataataagcc caaaaggagg tattcaatgt taagcatttt caaaacaccg
     7621 gtagagaaag aaacgctaga cgattgggct aaaatttcgg ttgatgtagc gaaagttgcg
     7681 attttggcaa ttcctgttat actttttggc aaagaaccaa cgttgattaa attgactaac
     7741 ttagcattat tggtattgag tatttatgtt tttttgacgc ttgggcgaaa attacggcag
     7801 ctaaaggagg tgttgtgatg gaactaggct ggaatttaac cataggtttg ctaattttaa
     7861 acgtctttgc gtttgcttgg ctttatatta ttaaccggat ttctaaagaa caattatcca
     7921 aaaatcaata atatttaatt tttcacaagc ggttgttttt gcaaaatatt ttgtaaattc
     7981 gaccgcttgc ctgtttttaa atgacaaaat acgacgataa aattcagcat gcatttagcg
     8041 aaaatgggtt gctcagccaa aatatcagtg gatttcgacc acgccaagca cagcttgaaa
     8101 tggctcaagt ggtcgcaaaa gcagtaaaat ttgcgacacc tgttgtggta gaggcgggaa
     8161 ccggcacagg caaaaccttt gcttatcttg tgccggcact actttcaggc aagaaaacta
     8221 ttctttcgac aggttctaaa aacctgcaag atcaactttt taatcgagat cttcccacta
     8281 ttcaaaaagc cctgaaatac aaaggcaaaa ttgcgttact caaagggcgt gcgaactatt
     8341 tgtgtttaga gcgtttagat caagtgaccg caatgggcgt acttggggat aaatccgttc
     8401 ttaccgattt aaaaaaagtc agtaaatggc aaaatgccac taaaacaggg gatttaagtg
     8461 aatgtattac cattgccgaa gatagcccga ttctaccgca gttagttagc accaccgaga
     8521 gttgcttagg ctcggactgc ccgcatttta aagattgtta tgtggtacaa gcccgccgta
     8581 aagcgatgga ggcagacgta gtggtggtaa accatcacct attctgtgct gatatggcag
     8641 taaaaaagac cggttttggt gagttgatcc ctgaagctga gctggtgatc tttgatgaag
     8701 ctcaccaact gccggatatt gccagccaat attttgggca atcgctttct tcccgccagt
     8761 tatttgatat ttgtaaggat accaatattg tttaccgcac tgaactcaaa gatctgtctc
     8821 aattaggcaa agcggctgat cacttgacga aagtagtgca agatttccga ttattgatgg
     8881 gcggagaagg gcagattcga ggcaatttgc gagagttatt tcgtgataaa aaagtggtcg
     8941 atggcttggt aaaagtggct gaaaatattg attttttaag tgaagtggcg aaaaaatcgc
     9001 tggggcggtc ggaaacgttg gataaaattt tcgagcggtt agcagaagtt aaggttcaac
     9061 ttaaaaaatt aatggatact agtgtgacag gctattgtta ctggtatgag gcgaatggac
     9121 gatcctttgg gctgcatatt acgccgctta tggtggcaga taaatttggt gaacagttga
     9181 agaaccagca aattggttgg gtgtttacct cagccacact cgaagttggg ggcaaattcg
     9241 atcatttttg tcaacggctg ggcatcgaaa atgcggagca gttggtgtta caaagtccgt
     9301 ttgattacca acatcaatct ttactctgtg tgccccgcta cttgccggat accaataaat
     9361 ccaatacttt aactgctctc gggcaattat taaaaccggt gattgaggcg aataaagggc
     9421 gttgtttctt gctttgtact tcctattata tgatgcgagg tttagccgac tttttacgcg
     9481 aacacagttc gctgaatgtg ttattgcaag gcgaaaccag caaaagccgt ttattggaaa
     9541 aatttgtgaa agaagatcac agcgtgttgg tggcgaccca aagtttttgg gaggggattg
     9601 atgtgcgagg cgatgattta tcgttggtga ttattgataa attgccgttc acttcaccgg
     9661 acgaaccatt actcaaagcc cgaatggaag attgtaagat acagggcggt gatccgttta
     9721 acgatattca aattcccgaa gcggtgatta ccctcaaaca aggtgtaggg cgtttaattc
     9781 gtgatgtgac cgatagaggt gtagtgatta tttgtgattc ccgtttggtg atgcgaaatt
     9841 atgggcaaac tttcttgaaa agtttaccgc cgtctagacg cactagagat ttggggaatg
     9901 ttattcaatt tttgaaacaa aaatagggtt acaagcggtc agatttctgc agatttttgc
     9961 aaaaaaacgg ataaaaataa ccgcttgttg aggagttata tgttgtctac attgctgcgt
    10021 atggttgctt ctttggcatt gttatttgtg atgctttatt taggcaagtt aatcgtttat
    10081 ctattcccca tcgggatccc ggatagtatt ttaggtatgc ttatcttatt cggctgcttg
    10141 gttatcggca ttgttaaagt ggaatgggtg ataccgagcg cgaaaccgct cacacgttat
    10201 ataacactat ttttccttcc gatttgtgtc gaactcatcg aacatcttga gctgttacgc
    10261 caaaatctga acacttttgt attagcaacg gcattaagca ccttagtttc tgtggtcgcg
    10321 attggcgtgt ttgcacagtg gatttttcat tacaagagag ggtaaaatga gctatcttta
    10381 cactgtgttg acggtaatca tgtttattgc cggtttgaaa atcagccaaa aactcaagag
    10441 cagttttctg aaccctttct taattgcact cgctttgcta attggtgtat tactgatttt
    10501 taatatccct taccaagact attatcaagg taattccccc ttgaataact tacttggcgt
    10561 ttctgttgtg gcacttgctt acccttttta tgaacaatta ccccatatcc gtaaaaaatg
    10621 gcgacagatt gtcgtgattt tgctgtttag taccatcatt acgatgataa ccgggcttgg
    10681 gttttcatta ttatttgggg caagccaaga gatcttagcc gccattttgc ctaaatcggt
    10741 atcaatgcca atggcaatta ttattacgaa tgaaattaat ggaaatgttg caatcgcagc
    10801 ggttggggtg atgatcgcag gcttagtggg atctatttta ggctttacgc ttctgcgcct
    10861 ggcaaaagta agaaatgtga tggtgattgg gttaagtatg ggagctgctt cccacgcatt
    10921 aggcacggca agatgcttag agcatagtat gaaagccggt agctatagtt ctatcgcatt
    10981 agtggcttgt ggtgtgctgt cttctatatt tgcaccttta atatttaacg ttatcgtcac
    11041 gttatgttac taaaaacagt gaaatctcaa accttatttg gtaactggct gaaatcaagg
    11101 tacaataccg tgatttttca atgaaaatag agaattatgc aagaacaaat aacacaagaa
    11161 aaagatcttt ctacgcttaa aacctttaag cgtttatggc cgactatcag cccgtttaaa
    11221 gcgggtctga ttacaggtgc tgtcgcttta gtattaaatg cgttagtgga ttctagtctt
    11281 atctatctcc tcaaaccctt gctagatgaa ggctttgggc aagctaataa tgattttctg
    11341 aaattgatgg cggtgcttgt agtagttttt attttagtgc gcggtattac caattatatt
    11401 gccagttact gtttagcttg ggtgtcaggc aaggtggtaa tgacattacg ccgccatatt
    11461 tttcaacatt taatgtatat gccggtaagc tattttgata acaacccgac cgggcgtttg
    11521 ctctcacgag tgacttacga taccgaaatg gtggcgagtt ctgcttctca ttctttagtg
    11581 actattgtgc gtgaaggggc ttatctgctg tcattgtttg tggtgatgat ctataccagt
    11641 tggcagcttt ctattgtgtt atttgtgatg gctcctatca ttggggtact gatcagtttc
    11701 gtttcaaaat attttcgtca attaagtcgc aatatccaaa gttcaatggg ggagcttact
    11761 gtcacgacag aacaaatgct taaagggcat aaggttgtac tttcctttgg tgggcaggaa
    11821 attgagaaag cacgttttga tcgagtcagt aatgatatgc gtcgtaaagg gatgaaagtc
    11881 gtttctgctg atggcatttc tgatggtgtg gtacagctta tcgcctcttt tgcgttatct
    11941 gctgtgcttt atgttgcgac cttccccgag gtgatgagtg agaatttaac cgcaggttca
    12001 ttcacagtag tgttttcttc gatgatggca atgttacgtc ctctaaaatc actcaccaat
    12061 gtgaactcgc aattccaacg tggaatggca gcctgccaaa cgttatttga atttttagat
    12121 ttaaaaactg aaaaagatca cggcttaatc tccgttgcta aagctgaagg caatgtggaa
    12181 tttaaaaagg taagctttag ttatgaaggt aaagaggaaa aagcactcga taatatttct
    12241 ttcaccattc ctaaaggcaa aacagtagca ttggttgggc gttccggctc gggtaaatca
    12301 actattgcca atttgcttac ccgtttttat gatgtgactg acggtcaaat tttgcttgat
    12361 ggaattaata ttcaagatta ccgcttaaaa aacttaagag aacaatgctc ggtggtttct
    12421 caacaagttc atttgtttaa cgatactatt gctaataata ttgcttatgc tgcaatcgac
    12481 aaatacagcc gagaagaaat tattgctgct gcaaaagctg cacacgcaat ggaatttatc
    12541 gaaaaattgg aaaatggctt agacactgta attggtgaaa atggcgcaag tctttccggt
    12601 gggcaacgcc aacgcttggc aatcgctcgt gcattactgc gaaattctcc tgtgcttgtg
    12661 ctggatgaag ctacctctgc attagatacg gagtcagaaa gagcgatcca atctgcactt
    12721 gaagtgctac agaaagatcg tacggtgttg gtcatagcac accggttatc aacgattgaa
    12781 aaagccgatg aaattttggt agtcgatcac ggaaatatta ttgagcgtgg cagccatacg
    12841 gaattgttag cacaaggtgg ggcttataaa caactccata gtttacaatt cggagcctaa
    12901 tttgtagggg agaaatatgt ttctccccaa ttaatgataa aaacagggcg aaaaatattt
    12961 cgtccctaca ataatttatt tggatactat gaaaaaactg acaaaacttt tatctcttag
    13021 tgctttactg gtcttaaccg cctgtaattc ggtactaaat gcctcaacag aggtgcaaca
    13081 accaacagta caaattccgc atgacgatcc acaatggcag cagcatttag cgaaactgag
    13141 ccaaattcaa ggctatactg caacggggca atttggctat atttcgcctg aagagcgttt
    13201 ttcttcccat ttcaactggc gttatactaa tccaatccaa tttgggttag agctttcctc
    13261 gaatctctcg actaaatcac tgaaattaga gcgtaataaa cgaggcttaa cggtttcgga
    13321 caatgagggt aatagccgtt ctgaccgtga tgttgatgcg ttaatgcaag agattatcgg
    13381 tgtttcgttt cctatcgatc agtttgccta ttgggtaaaa ggcttgcctg aaaaagatgg
    13441 caactatatt gtgaatgaaa aacgccagct gtcgcaattt agttatccgc ttaatggcac
    13501 gctttggaaa gcaagctatg tggagtatca cgaagatcgt gtaccaaact taccgaagct
    13561 gattgtgctt gaaaacggca ctcaaacgtt gaagatccgc attgaaaaat gggcgtatta
    13621 atgaagaaag tcaccttacc cagcccggca aaattaaacc tgtttttata cattaccaat
    13681 aagcgggcag acggttatca cgagctacaa acgctgtttc agtttttaga ttttggtgat
    13741 gaaattgaga ttgagatcac agaaagttca gaggttgaat taattaatcc ggtagaaggg
    13801 gtggcaactg aggagaatct catttaccga gcagcaaaac ttttgcaaaa aacgaccgct
    13861 tgtaggcaag gctgccgtat tgggattaaa aaaaacctac cgatgggcgg aggtgttggt
    13921 ggtggatctt ctaatgctgc aacagtatta gtcggtttaa atcacttgtg gaataccgag
    13981 ctttcattgg aaaaattggc agaattaggc ttaagtttag gggctgatgt gccgatattt
    14041 gttagaggta tggctgcttt tgccgaaggc gtgggggagg tgttaactcc ttgtgatcca
    14101 caagaaaaat ggtatgtagt gctaaaacca gaagtgtcga tttcaaccgc agcggtgttt
    14161 accgacccga atttacctag aaatacgcca aaacgcccct tagccgaatt attatcttta
    14221 ccgtgggaaa acgattgcga aaaagttgta cgagaaaagt attctgcggt tgaagatctt
    14281 atacaagaat tgttacaata tgctcagttt aggctaacag gcacaggtgc ttgtattttc
    14341 gccgaatttg aaacaaaagc agaagccgag caggtttttg cacataaacc ggaagccgtc
    14401 ttcggttttg tggcgaaagg gcaaaatatt tcgccattac atcaaatgtt attccaaatc
    14461 taaccatctt aaccttaagg atcaatccat gacagatatt aaactgtttg cgggcaacgc
    14521 aactccggaa cttgctaatc gtattgcaaa acatctttat acctcattag gtaatgcaac
    14581 agttggtcgt tttagcgatg gtgaaattca agtacaaatc aatgaaaatg tgcgtggagg
    14641 cgatattttt attgtgcaat ccacttgtgc accaactaat gataatttaa tggaattgat
    14701 cgtgatggta gatgcgcttc gccgtgcatc agctggtcgt attaccgcag tgattccgta
    14761 tttcggttat gcacgccaag acagacgcgt gcgttcggcc cgtgtgccaa ttaccgcaaa
    14821 agtgattgct gatttcttat ccagcgtagg ggtagataga gtcttaacct gcgacttaca
    14881 cgcagagcaa attcaaggtt tctttgatgt gccggtagat aacgtatttg gttcccctgt
    14941 gttaatcgat gatattttga aaaaatccga tttggtcaac cctatcgtgg tttctccaga
    15001 tattggtggg gttgttcgtg caagagcggt ggcaaaatta ttaaatgata ccgatatggc
    15061 aattattgat aaacgtcgtc caaaagcgaa tgtatcacag gtaatgcaca ttatcggtga
    15121 tgtggctgat cgtgactgta ttttagttga tgatatgatc gatactggtg gtactttagt
    15181 caaagcggct gaagcgctga aagaacgtgg cgcccgtcgt gtctttgctt atgccacaca
    15241 cgcggtattt tcaggcacag cagctcaaaa tttagcgaat cctgctttag atgaaattgt
    15301 agtaactgat actattccac tgagtaatga gattaaagca ttaaataaag tacgtgtttt
    15361 aacattatcc ggtatgttag cagaagcaat tcgccgtatt agcaatgaag aatccatttc
    15421 agcaatgttt gatgcctaat ttatgagaaa aaacccagtc gaaagattgg gtttttcata
    15481 tctgttcact actatattta gtttaagata atttgtatgt attccctgat tcgtaaatta
    15541 ctttttgcac tagatacgga aaaagcacat caattctcta ttcaggcact tgctttagcc
    15601 ggaaaatcgc ctttttcgat tctgccaaca cccgataatc cgacccaagt aatgggactc
    15661 acgtttaaaa atccaatagg gcttgcagcc ggagcggata aaaacggtga agcgatagat
    15721 ggttttggga aattaggatt tgggtttatt gaggttggga ctattactcc tctcgctcaa
    15781 gacggaaacc ctaaaccgag gcaatttcgt attgtagatg cagagggaat tattaaccgc
    15841 aatggattta ataataaagg cgttgatgtt ttagtagaaa atgtaaaaaa tgcaacctat
    15901 agtggtattt taggtattaa tattggaaaa aatgcggtta caccaattga gcatagcttt
    15961 gatgactacc aaatttgttt gcgtaaggtt taccaatatg ctgattatgt ggcggtgaat
    16021 atttcatcac ccaataccaa aaatttgcgc agtttacaat atggtgaggc attagatgat
    16081 ttgctgcgaa acctcaaaaa tgagcaggca caactttcgc aaaaaatggg gaaatatcga
    16141 ccgcttgttt taaagattgc gcctgattta accgatgaag aaatcgtttc tgtagccgat
    16201 agtcttattc gttaccaaat tgatggggtg attgccggca atactacctt gtctcgtgaa
    16261 agtattgccg gattaccgta ttcggaacaa ctgggtggat taagtggcaa gccattacac
    16321 tcattaagta caaggttaat ttcaaaatta tctgccgagt tgggcgagag aataccaatt
    16381 ataggttgtg ggggagtcca ttcgttagag agtgggcagg agaaagtgaa tgcaggtgct
    16441 agtttattgc aagtctattc cgcaatgatt taccaaggac catcattaat tcaaacctta
    16501 gccaaacacc ttcagctgaa cagttaggga tagatttcta gttaaaaatg attggttagg
    16561 tttatttctg tcatcttagt ggaatagatg gcggtattag ctatctatca aaaatagaca
    16621 aaattg
//
LOCUS       DANLXF010000035        16321 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.035, whole genome shotgun sequence.
ACCESSION   DANLXF010000035 DANLXF010000000
VERSION     DANLXF010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16321
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.035"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(1..747)
                     /locus_tag="PXD66_000676"
                     /pseudo
     CDS             complement(1..747)
                     /locus_tag="PXD66_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251293.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            complement(747..926)
                     /locus_tag="PXD66_000677"
     CDS             complement(747..926)
                     /locus_tag="PXD66_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HDL6319004.1"
                     /translation="MLKVKAAKGVRVPVENRPHHYIEQEAVEVENTLYYQRRIADGDL
                     IVVKETKSAKETGGK"
     gene            complement(927..1568)
                     /locus_tag="PXD66_000678"
     CDS             complement(927..1568)
                     /locus_tag="PXD66_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HDL6319005.1"
                     /translation="MISKIERALIERLTLGLGKMVYAVAGYAGEINDEKLDVLRLPAC
                     LVSYGGSAFEAKSMGVRGKRYQATDVFVVLVITRSMRNQTAGRLGGVTSREVGVNQLL
                     GAVKTLLINQTLDGLVHPIQPKRIRTIWNNAEVKKEKLSAYAIEFEISYNECNVLDDG
                     RFPEGVEPNEQVFKQYQGKLDEPQGDLSGFNNRIFDPNNNAETAFRTEFKDGD"
     gene            complement(1565..1990)
                     /locus_tag="PXD66_000679"
     CDS             complement(1565..1990)
                     /locus_tag="PXD66_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 domain-containing protein"
                     /protein_id="HDL6319006.1"
                     /translation="MYALVDDFVLRIGEQESIELTDREMVGVVDEATLEVALADSSSQ
                     IDGYLSGRYRLPLKTVPQNLKRICCDLARYHLTSKSLVTMTEEVENRYKLCLKELENI
                     SKGVVSLAVDETNEEESATGENTVQFFNGGNRIWGRDKR"
     gene            complement(1990..2331)
                     /locus_tag="PXD66_000680"
     CDS             complement(1990..2331)
                     /locus_tag="PXD66_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319007.1"
                     /translation="MAKPKKNDNEALNTNENTPLEDKVNEQPEDKTDEQPKPPESETK
                     PDDGALINPIAFEVKLKSIHPQATYGRCGYRFNKETAVEIARKALSDEQIITLANDPY
                     LEFVPVVEGDA"
     gene            complement(2379..3296)
                     /locus_tag="PXD66_000681"
     CDS             complement(2379..3296)
                     /locus_tag="PXD66_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HDL6319008.1"
                     /translation="MFKKSELLKALDTAFRKEFSAGLKVLEPQWGTVAMKVSSSTATN
                     TYAWLGQFPKMQEWVGDRQIKNMQAQGMTIENKLFESTVAVPRTAIEDDQVGLFTPMV
                     KQAAQSAAELPDDLVFSLLKKGKTTLCYDGQNFFDTDHPVYQNVDGTGTSKTQSNITT
                     GSASGKPAFYVLDDSQAIKPLIWQERTTPEIETKFDPSESDHVFMKDQYLWGVRARGN
                     AGFGFWQLIHRVEDSELNSETLEKVLTAMRTLKGDGDKQLNIRPTTLLVPPSLEFAAR
                     KLLEAELIDGTTNTLKGVLKVVVNPFIAE"
     gene            complement(3296..4366)
                     /locus_tag="PXD66_000682"
     CDS             complement(3296..4366)
                     /locus_tag="PXD66_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HDL6319009.1"
                     /translation="MFKHKFKLNPIALSFKLNQEANGRIQLFPFGWFEAQDGRGGRWY
                     VGDENGYALADDINNTAIDLMIDYEHQTLYIAENGKGNPAAGWITKAEYISGEGLFAD
                     VKWTPQATQEIKDGVYRYLSPLFLTDGSGMVIKVLNAALTNRPALHNLQEVVAMSSQF
                     AKFLTPEEENPKMKELLIKLFALSANATDEQITEKLTALEKAKGDSPVALSDVYTELA
                     KEKSQVVALSAKVNHPDPAKFVALSDLQAVQTELNGLKQQINDKERDALIQTALSDGR
                     LLPAQKAWAEKLGKQDLVALSDFLKTVTPNPALASTQSGGEDPNKGTEKVALSAAEIA
                     AAKSLGLSEAAYIEKYKTEESK"
     gene            complement(4626..5042)
                     /locus_tag="PXD66_000683"
     CDS             complement(4626..5042)
                     /locus_tag="PXD66_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HDL6319010.1"
                     /translation="MIEIQIHGIDEIIATLNRIAAKTANKTDLMRNIAGKMESAVLTN
                     FDEGGRPKWLGIKHRQGTPLVDTENLMGSITADYNNDSAIVGTNEPYAAIHQFGGMAG
                     RGRKVKIEARPFLQLTSQDRQDILDDVQAYWQKLLD"
     gene            complement(5193..6492)
                     /locus_tag="PXD66_000684"
                     /pseudo
     CDS             complement(5193..6492)
                     /locus_tag="PXD66_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248654.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
     gene            complement(6479..8104)
                     /locus_tag="PXD66_000685"
     CDS             complement(6479..8104)
                     /locus_tag="PXD66_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HDL6319011.1"
                     /translation="MSKKKHNKQIKPNLNPIKNQELQTDLAEITATGRVLSDHPSNFI
                     TPAKMKAIFEDTENGDITAQHELFMDIEERDSSIFANIQTRKRAALGVDWAIVAPRNA
                     TPQEEKLRDEVDELFYQLGNLEDLVMDCMDAVGHGFAALEIEWAFNGKLWYPNAFIHR
                     PQSWFKWDKHDNLLLKTRQNTEGEPLREFGWVVHTHKSRSTQAARNNLFRSLAWLYMF
                     KHYSIHDFAEFLELYGMPIRIGKYGAGATPNEKQTLKRALAEIGHNAAGIMPESMSIE
                     LHNAANAGGAAGNNPFLQMVDWCEKSIARLILGQTLTSGADGKSSTNALGNVHNEVRR
                     DLLVSDVKQLGQTFTQQIILPFLQINFGNIDPTRIPSFEFDTKEPADLALFAESLPKL
                     VDIGMPIPVQWAMDKLGIPEAQENEAVLGRSAPPQAVGLSANFPQKMTACECGCGGKV
                     HALSAQTKTGIKEQDALDDLADEAFSVPDFNAQFDPITKKAVAVVMACSSYDEAAEKL
                     AAAYPDLVSEEHDRYLANALFLADLLGAANAES"
     gene            complement(8173..9798)
                     /gene="terL"
                     /locus_tag="PXD66_000686"
     CDS             complement(8173..9798)
                     /gene="terL"
                     /locus_tag="PXD66_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HDL6319012.1"
                     /translation="MKIKEFEKELEELRRQLQQNIEASFEGWDDKPQAISERRQKVLD
                     PVHGFEYFVQAYFPHYVRSEHKSQLHHYLFENLPLSVSDLSKSVRQAIAAPRGEAKST
                     ICTQLFPLWCLVRDLKRYIIIAMDTKEQAYSMLEAIKVEIESNPRLRIDFPEVAQGKV
                     WRAGVIMTAKKQKVEAVGAGQKLRGRRHGAYRPDLVVLDDIENDESVETPAQRNKLHN
                     WVLKAVLKLGAAGEKFDVIYVGTILHYDSVLSRILNTKGWRRVRFKAILRFPGNMALW
                     DEWENIYLSEEGDDDTMSDLFYQQHKAEMDKGALVSWLARPILYLMKIRASDGHSAFD
                     SEYQNDPVSGDDAIFANSIQYWTELPDDLIYFGALDPSMGKAGASRDPSAILVGGYHR
                     ESGKLYVVEAQIKKRLPDLIIEDVIRLHSQYNCHRWFVETVQFQEFLQTELVKRSAQR
                     GKPVPATATKPNSDKMLRIESLQPHIANGLILLHRSQSTLESQLRHFPKADHDDGPDA
                     LEMLWRNALSSSAPIEWESVDDFDGEYRSKWRH"
     gene            complement(9802..9948)
                     /locus_tag="PXD66_000687"
     CDS             complement(9802..9948)
                     /locus_tag="PXD66_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319013.1"
                     /translation="MAKKKQTVVHKHYSFRWIVQLACIFGAIKLMLNGTDGWGWLLLI
                     AACM"
     gene            complement(9948..10448)
                     /locus_tag="PXD66_000688"
     CDS             complement(9948..10448)
                     /locus_tag="PXD66_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HDL6319014.1"
                     /translation="MAHDVEVQKAVRQAYVFDRLSLEMAAERAGVSFGTARRWKAAAE
                     KNGDNWEKARDVQAMASGGIENIAQGLLAGFLIKYRTLMTELEENTEMPTAAKVEALA
                     ALADSFAKMTASSKKLLPETSAMATAMRAIEMMANIVKTKKPHLLPDFLEMLDELEVQ
                     FKKEFK"
     gene            complement(10456..10710)
                     /locus_tag="PXD66_000689"
     CDS             complement(10456..10710)
                     /locus_tag="PXD66_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319015.1"
                     /translation="MSRKKQKQTDSEKLDLILGGLNELNDKVDKQNEEIERLHREIQA
                     TNRLVNEMARKNRRDAVVAGGLGGGIVAVGFELLRLKFGM"
     gene            complement(10710..10967)
                     /locus_tag="PXD66_000690"
     CDS             complement(10710..10967)
                     /locus_tag="PXD66_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319016.1"
                     /translation="MIDEKLFQMLISFVVAPLVAFVGKVIFDRINRNEQTVKELKAEM
                     ESKYQSKELAHEVNRGIKEKLDDILDSLKEVNQKLDKKADK"
     gene            complement(10960..11115)
                     /locus_tag="PXD66_000691"
     CDS             complement(10960..11115)
                     /locus_tag="PXD66_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319017.1"
                     /translation="MKLIKKLRQRWQQWRFYRRRPDLAELHNVVRAAVAQGNQHPVGR
                     AKRGRYD"
     gene            complement(11130..11489)
                     /locus_tag="PXD66_000692"
     CDS             complement(11130..11489)
                     /locus_tag="PXD66_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2681 domain-containing protein"
                     /protein_id="HDL6319018.1"
                     /translation="MITQQIAGVVGVVSTILAYAFYKSWQLKQERKLTATLKAEKQQQ
                     AVEIQQKNAEVKNAKIQQKHVSETRRIQPSAIDEQLHQHGYFRDDNGLHGVRSDLPEP
                     CGHNGNETPSACTQSDL"
     gene            complement(11486..11731)
                     /locus_tag="PXD66_000693"
     CDS             complement(11486..11731)
                     /locus_tag="PXD66_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2644 domain-containing protein"
                     /protein_id="HDL6319019.1"
                     /translation="MKQFLLDLITNKDGTASTTGFIQFMSWLVLSGILIHAYLYDKPF
                     ISDWWFAYAGICVLGSPATKGVVSALKQKERSREAEE"
     gene            complement(11734..12267)
                     /locus_tag="PXD66_000694"
     CDS             complement(11734..12267)
                     /locus_tag="PXD66_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HDL6319020.1"
                     /translation="MTEPIKNKATIADFNLAFDRLIGHEGGYVNDPKDPGGETNWGIT
                     IHTARANGYTGSMRAMSRADAKTIYLKAFWQRYNCEQFPPELAFQFLDACVNHGSGNA
                     SRMLQRAVGVADDGIIGKVTLAAIERYSVADVGLLFQAERLEFYTKLKHFDRFGRGWI
                     NRMAGNLRYFAKDTVEE"
     gene            complement(12353..12778)
                     /locus_tag="PXD66_000695"
     CDS             complement(12353..12778)
                     /locus_tag="PXD66_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mor transcription activator family protein"
                     /protein_id="HDL6319021.1"
                     /translation="MSEFESVEHYLPETVKEIVGVIGLPATEKLIKAFGGFSFQFSNG
                     KLYFNKLKEVLGQDDAVKLQAYMGACEVYLPRCETALRMLRNQQIYADYCQLTEQGGL
                     SGRLAIMQICPKYSVCDRVAWEAVRYYQRKHTVSQATLF"
     gene            complement(12849..13064)
                     /locus_tag="PXD66_000696"
     CDS             complement(12849..13064)
                     /locus_tag="PXD66_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL6319022.1"
                     /translation="MIENPEFGYTPSNLKMLRKHHNLTQTEVANILGVGQRIVMRWET
                     ELSSTAARSDMPYTKWAELLKNLQKNF"
     gene            complement(13083..13475)
                     /locus_tag="PXD66_000697"
     CDS             complement(13083..13475)
                     /locus_tag="PXD66_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319023.1"
                     /translation="MTDYQKLLNALLNDADEDCFAVYGLRATTDVHQVGDELGNSFVW
                     VDGEKTDEELDGICTMGIQNADEAGLLNAIKNLGRDACKKFDVEFKGFQSYCGQNFIL
                     VKGDSARGGEDKGESIIRNPIVVAVFNS"
     gene            complement(13601..13948)
                     /locus_tag="PXD66_000698"
     CDS             complement(13601..13948)
                     /locus_tag="PXD66_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319024.1"
                     /translation="MKELLLTTLCLLSLPAIAMTEKAEQETANALVSGDYQQLRNVAY
                     GMETGSFGHDHNPIAACALRRVILLVNSDKVDMTDFNNEAIACRKIEVTDNQQAWETA
                     FTIAKSISATKKK"
     gene            complement(14037..14462)
                     /locus_tag="PXD66_000699"
     CDS             complement(14037..14462)
                     /locus_tag="PXD66_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein GemA"
                     /protein_id="HDL6319025.1"
                     /translation="MQPQTRKQMIQKIHIGKNELKLDDNAYKMLLLEAVDKPSCSMMT
                     DSELMTVLQTMKAKGFVVKSKKYGKRPTASKTAPHRQGLINKIGALLYQSQKPWDYAH
                     AIAKRSFGIERVQWLTDDQLHKLVQILASYNHRHGMSVK"
     gene            complement(14446..15000)
                     /locus_tag="PXD66_000700"
     CDS             complement(14446..15000)
                     /locus_tag="PXD66_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319026.1"
                     /translation="MKLCKCPICHSDISFEALIEDDAGRELLNTIVNIGGNCGRAVVA
                     YLGLFKPQKNSLANSRALKIVQSLLELYPPSNVLEKALMDTVEQVRRNRRETGRCEPL
                     ANHNYLKKVYESVKPQFAVVRSEAKAVSSLEQQQRQADQKKHDAVMYVERMKQMGQLE
                     AVKNTESYQIWLKWQEEKQNATAN"
     gene            complement(15000..15170)
                     /locus_tag="PXD66_000701"
     CDS             complement(15000..15170)
                     /locus_tag="PXD66_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319027.1"
                     /translation="MEYQKIPEYRLCKKKSGEIVLQQLVICELTPATWHNPYPDSRTY
                     WVDLETIEEGTE"
     gene            complement(15241..15816)
                     /locus_tag="PXD66_000702"
     CDS             complement(15241..15816)
                     /locus_tag="PXD66_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5420 family protein"
                     /protein_id="HDL6319028.1"
                     /translation="MKFRFFKGDITQEPLKSIYADYQVSKEERKDKLNAILEKYPFND
                     GLISRSGWFHRIVIGIACKATNISQVLDVKGFKVGKFNDEFYLVKPDKRYEKGKELAE
                     DFKAINEIYEKHRDFSPYILKRLNMLYFVSDFSYSPTSGRSYLAVAGICENTLLVEMP
                     MPIDKNDEPFPEIAEGLVEIKESEFLAIQGK"
     gene            complement(15839..16021)
                     /locus_tag="PXD66_000703"
     CDS             complement(15839..16021)
                     /locus_tag="PXD66_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319029.1"
                     /translation="MTEQEKNELNSQLNEALMQIIQAQKYLKQSDFIRSGVYLGTVQD
                     LLPKVHLKLLTANRKH"
     gene            complement(16088..16234)
                     /locus_tag="PXD66_000704"
     CDS             complement(16088..16234)
                     /locus_tag="PXD66_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HDL6319030.1"
                     /translation="MKTEQKATEFDRYKHYAEKAAEAERKGNYAEAQDHWKVAKLSAK
                     KTAN"
BASE COUNT         4297 a         4032 c         3049 g         4943 t
ORIGIN      
        1 ccgcaccaag ccctgccgca atcattgcat ttgattcggt tgctccctta taccagccta
       61 caataattcg ctcactgtta aggcttgcgg ttaaggttgt gccggtggca taagtgccac
      121 gccagcccat tacaccaata gccggtttat cttcaatcgg ggcagaaacc gattctaaat
      181 ggctacgcaa ggcaagggca ttttcatcat ctgaaaacgg cgaaataatc acgttataat
      241 gcgtaccggc aacactcgcc aacgccggag caattagggc attacgctga ccgttatcaa
      301 acgcacgagc ctcaagggtt aaaccatcgc ctgtgcttct tcgccttttg cggctgcaac
      361 ttcgtaagct gtgccggcaa tcgtaatacg caccacaccg gcataagtta ctgtgccggt
      421 aaaactaact tgccctgttg ctgcaatacc tgcctcgtgg tcttttaagc ccaccaccgt
      481 taaacggatt agcggattat tttggattgc ttggcgaacc attaaatgag ctaccgagcc
      541 tttaccaaat aaattttccg cctccacatc gctaaaaatc ttttgcggag cagtaaaagc
      601 agcctcaccg tttagcattg gtgcaacaat tagcacctct tgctcattgg tcggcaaggt
      661 ggttactgca tctttgttgt tgtattcggt cagcacactt ggtttgcgaa tactgttcgg
      721 gattttctca aattcaattt ttgacattat ttgccccctg tttctttagc ggatttggtt
      781 tctttgacga caatcaaatc gccatccgca atgcggcgtt gatagtaaag ggtgttttca
      841 acctctactg cctcttgttc aatatagtga tgcggtcggt tttctaccgg cacacgcacc
      901 cctttagcgg ctttcacttt taacatctaa tcaccatcct taaattctgt tctaaatgcc
      961 gtttcggcat tgttattcgg gtcaaaaatc cgattattaa agcccgataa atcgccttgc
     1021 ggttcatcta gcttgccttg atactgctta aagacttgct cattcggctc aaccccttcg
     1081 gggaatctac catcatcaag cacattgcac tcgttgtagc tgatttcaaa ctcaatagca
     1141 taagcagaga gtttctcttt cttcacttcc gcattattcc aaatcgtgcg gattcgctta
     1201 ggctggatag ggtggactaa accgtctaaa gtctggttaa tcaataaggt tttcaccgct
     1261 cccagcaact gattaacccc cacctcacga cttgttaccc cacctaaacg cccagcagtc
     1321 tgatttcgca ttgagcgagt aataaccaac accacaaaca catcagtggc ttgatagcgt
     1381 ttacctctta ctcccattga tttcgcctcg aacgccgagc caccataaga gacaagacaa
     1441 gcaggcaaac ggagaacatc taacttctca tcgttaattt ccccggcata cccagcaacg
     1501 gcatacacca ttttgcctaa gcccagcgtt aaacgctcaa tcaaagcacg ctcaattttg
     1561 ctaatcatcg cttatcacgc ccccaaatac ggttgccccc gttaaaaaac tgcactgtat
     1621 tttcgccagt agccgattct tcctcattgg tttcatcaac cgctaacgac actacgccct
     1681 tagagatgtt ttccagctct tttaagcaaa gtttgtagcg gttttccacc tcttcggtca
     1741 tcgttactaa tgatttgctg gttaaatgat aacgagccaa atcacagcaa atacgcttta
     1801 aattttgcgg tacagtcttt aatggcagac ggtaacgccc acttaaatag ccatcaattt
     1861 ggctggaaga gtccgctaaa gctacttcaa gagtcgcttc atcaaccacg cctaccattt
     1921 cacggtcggt tagctcaatg gattcctgtt cgccaattcg caaaacgaaa tcatcaacca
     1981 acgcatacat tacgcatcgc cctcaacaac aggtacaaac tccaaataag ggtcgttggc
     2041 taaggtaata atttgttcat cactcaaggc tttacgagca atttctaccg ccgtttcttt
     2101 attaaaacgg tagccacaac gcccataggt ggcttgtggg tggatagatt tgagcttaac
     2161 ttcaaaagca ataggattta tcaaagcacc gtcatcaggc ttagtttcac tttcaggcgg
     2221 tttaggttgt tcatccgttt tatcttcagg ctgttcattc accttatctt caagcggtgt
     2281 gttttcgttt gtgttcaaag cttcattgtc atttttcttt ggttttgcca ttttgctacc
     2341 ctctcaaaat gggcgtattg ctacgcccaa cattaaactt attcagcaat aaatggattc
     2401 actaccactt ttaacacacc ttttaaggtg ttggttgtgc cgtcaattaa ttctgcttcg
     2461 agtaatttgc gtgcagcaaa ctcaagcgaa ggtggcacaa gcaaagtggt cgggcgaata
     2521 tttaactgct tatcgccatc gccttttaag gtgcgcattg cagttaatac tttttcaagg
     2581 gtttcactat ttaactcact atcttcaaca cggtgaatta actgccagaa accaaaaccg
     2641 gcattaccac gagcacgcac accccataaa tattggtctt tcataaagac gtggtctgac
     2701 tcacttggat caaacttcgt ttcaatttca ggtgtagtac gttcttgcca aattaacggc
     2761 ttaatggctt ggctatcatc tagcacatag aacgcaggct taccgctggc agagcctgtg
     2821 gtgatattac tttgtgtttt acttgtgcca gttccatcaa cattctgata aacagggtga
     2881 tcggtatcaa agaagttctg cccgtcatag caaagggttg tcttaccttt tttcaacaaa
     2941 ctaaatacca aatcatcagg taattcagcc gcactttgtg ccgcttgttt caccattggg
     3001 gtaaataagc ccacttggtc atcttcaatg gcagtacgag gcacagcaac ggttgattca
     3061 aataatttat tctcaatcgt cattccttgt gcttgcatat ttttaatttg acggtcgccc
     3121 acccactctt gcattttcgg gaattgtccc agccaagcat aggtattagt agcggtgctg
     3181 gagcttacct tcattgcaac cgttccccat tgtggttcaa gcacttttaa accagcacta
     3241 aattctttac gaaatgcagt atcaagtgct tttaataatt cagatttttt aaacattact
     3301 tggattcctc tgttttatat ttttcaatgt aagctgcttc gcttaaacca agcgattttg
     3361 ctgctgcaat ttccgcagca gataacgcca ctttctccgt acctttgttc gggtcttcac
     3421 cccccgactg cgtacttgct aaagcggggt taggtgttac cgttttcaaa aagtcggata
     3481 aagccactaa atcttgctta ccgagctttt ccgcccacgc tttttgagcc ggcaataacc
     3541 gaccgtcaga aagagcggtt tgaattaacg catcacgctc tttatcgttg atttgctgtt
     3601 ttaagccgtt cagctcggtt tgcactgctt gcaagtcgct taacgccaca aacttcgccg
     3661 ggtcagggtg attcactttg gcagagagtg caaccacttg gcttttctct tttgccagct
     3721 cggtataaac atcacttaac gccaccgggc tgtcgccttt ggctttctca agtgcggtca
     3781 gtttttcggt aatctgctca tcggtcgcat ttgccgacaa tgcaaataat ttaattaaga
     3841 gttctttcat ttttggattt tcctcttcgg gggttaaaaa tttggcaaat tggctagaca
     3901 tcgcaaccac ctcttgcaga ttatgcaagg cagggcggtt agttaaggcg gcattaagta
     3961 ccttaattac cattccgctg ccgtctgtca aaaacagtgg cgagagatag cgataaacgc
     4021 cgtctttaat ctcttgggtc gcttgtggtg tccacttaac atccgcaaac aagccttcgc
     4081 ccgagatata ttccgccttg gtaatccaac ccgcagcagg gttgcctttg ccgttctcgg
     4141 cgatgtacag ggtctgatgc tcataatcaa tcatcagatc aatcgcagtg ttgttaatat
     4201 catcggctaa cgcatagcca ttctcgtcac ctacatacca acgtcctcca cgcccgtctt
     4261 gagcctcaaa ccaaccaaaa gggaaaagtt gaatacgtcc atttgcctct tggttgagtt
     4321 taaaactcaa tgcaatgggg tttagtttga atttatgctt aaacacaaaa acacctcaaa
     4381 acaaattaat gaggctaaag aatagaagat tgagaaaaaa gagtaacggg gagcaacttc
     4441 cacaagttgc aaattttcag aaaaaaaaca accgcttgta ttcagcgagg aatgtaaccc
     4501 attttaaaac gctttaaatc cgttttaaaa aattttaaaa aagttggaat gataaatcac
     4561 cataaaaaaa acaaaaacgc cacagagagc gatttagggg ctatctgtgg cgattatctt
     4621 atcgcttaat ccagcagttt ttgccagtag gcttgcacat cgtccaaaat atcttgcctg
     4681 tcttgcgagg ttaattgtaa aaaaggtcgg gcttcaattt ttactttacg cccacgccca
     4741 gccataccac cgaattgatg aatagcagca taaggctcat ttgtgccaac aatcgccgaa
     4801 tcattattat aatcagcagt aatgctgccc attaaatttt ccgtatcaac taacggcgtg
     4861 ccttgccgat gttttatacc cagccatttc gggcgaccgc cctcatcaaa attggtcagt
     4921 accgccgatt ccattttgcc ggcaatattc cgcatcaagt ctgttttatt tgccgtttta
     4981 gcagcaatac ggttaagcgt ggcaataatt tcatcaatgc cgtggatttg gatttcaatc
     5041 atttttaacc ttgcaagttg atttttaaaa taaacagggg tatattcctt ttacggttat
     5101 tagaaaagcg gtgataatct ccctgccgca gcctatttga gatggcaagt cctgctcaaa
     5161 tgaccgagag cgtgtgtaag tgaccgggag ccttacctaa taaccgtatt tttcaacagc
     5221 agcttatcca attctttttg tccaatctcc cttgttgata ccagaaataa ttcaccaaac
     5281 tcttctaagt atttcaccac aacaagaata cgataatgct caaagtttcg tataaagctg
     5341 aaatttttgc cttcttgcaa aatatgatta ggctgaaaaa ttacatcagg aatatttgca
     5401 tacacatcaa acccaaaatc ctgcccctca cggctataaa actgcttaat cagtgtgtca
     5461 tccgaaagcc ataccgtgcc gactttactg tcgattaagc gttgtgtttc ctcacttaac
     5521 cgaccagcag caaatttaaa gttttgatta agggcatttc gcactttaac catctcatca
     5581 ggcgagagtt ttttcgcccc atctttggca agtgccacag cctcttctaa cttgttaaac
     5641 gcctgcttaa attcaccacc gctcatttct gctttggtaa attggtaagc cagcttttca
     5701 gggtataaat ccaaattcgg cttataggtt aaacgcccca cattataatc aaaaccttta
     5761 tcagccaatc gcaccgtacc atcagccaat ttaaagccaa tggtttcctc ttggttgcct
     5821 aacttatctt tcggtcgttt tgccttcact aaaaacggct cgctactgcc gacttcgtca
     5881 atccctttac gctttaaatc ccgctcactt aacgcaatca cggtacaacg gcaattaaag
     5941 ccgttcggcg gataaaaagt cgcccaaaac ggatcgtcat aacgataaat cttaccctgt
     6001 aatgcttggt gagcagggcg ggtgcgttgg tcgcctaccg ccgaatattg ccaataaggg
     6061 cgattatcca cattatccat ataccgctga taacgagccg cagaataagc ctgttgcata
     6121 ttggtacgat aaatcgtatt caagcggcga ggcgtgccga aatgctcgcc ggttttcggg
     6181 tcggcaagca gtttgccatc aatgccacga ctaatcgtcg tgtccataca cccagccttt
     6241 ccgttcaaat tctgccgtaa gcgttttctt ccattcatta aaggatttcc cctcacgccg
     6301 tgctttttcc atcgactcat aaatatcacg ggtcatttca aggctagtga gcttgctaat
     6361 ggtggcggct cgccccaatg cagaatcaaa taattcctgc ttaaacactt tattcgcaag
     6421 caacttcttc tgccgtaaaa actcaatcgc ctgcttaggc tctagtccaa tggcaaaatt
     6481 aactttctgc attcgcagcc cctaataaat ccgccaaaaa taacgcattc gctaaatagc
     6541 ggtcgtgttc ttcactcact aaatcagggt aggctgccgc caatttttct gctgcctcat
     6601 cataagagct acacgccatt accaccgcca ccgccttttt cgtgatgggg tcaaattggg
     6661 cgttaaaatc cggcacggaa aaagcctcgt ctgccaaatc atctaaggca tcttgctctt
     6721 taatgcccgt tttggtttgg gccgacaaag cgtgaacctt gcccccacaa ccgcattcgc
     6781 aagcggtcat tttttgcgga aaatttgcgg ataaccccac cgcttgtggc ggggcagaac
     6841 gccctaacac cgcttcattt tcttgagctt cgggaatacc gagtttatcc atcgcccact
     6901 gcaccggaat cggcattcca atatccacca atttcggcaa gctctcggca aataacgcca
     6961 agtcggcagg ctctttggta tcaaactcaa aactcggaat gcgggttggg tcgatattgc
     7021 caaaattaat ttgcagaaac ggcagaataa tttgttgggt aaacgtctgc cccagctgtt
     7081 tcacgtccga caccaacaaa tcacgccgca cttcgttatg cacattgccg agtgcattgg
     7141 tagagctttt gccgtctgca ccgctggtta aggtttgccc caaaatcaag cgggcgatag
     7201 acttctcaca ccaatccacc atttgcaaaa acggattatt gccggctgca ccgcctgcat
     7261 tagcggcgtt atgcaactca atactcatcg actccggcat aattccggca gcgttgtgtc
     7321 cgatttccgc taatgcacgt ttaagcgttt gtttttcgtt tggtgttgcc cctgcaccgt
     7381 atttaccaat gcggataggc ataccataca gctccaaaaa ctcggcaaag tcgtggatag
     7441 agtagtgttt aaacatatac agccaagcca gcgagcggaa taaattattg cgtgccgcct
     7501 gtgtggagcg ggatttatgc gtatgcacca cccagccaaa ttctcgtaat ggctcacctt
     7561 cggtattttg tcgggttttc agcaacagat tgtcgtgctt atcccactta aaccacgatt
     7621 gaggacggtg gataaaggcg ttcggatacc acaatttacc gttaaacgcc cattcaattt
     7681 cgagggcagc aaaaccgtgt cccacagcgt ccatacagtc catcaccaaa tcttcaaggt
     7741 tgccgagctg ataaaataac tcatcaacct catcacgcag tttttcttcc tgcggtgtgg
     7801 cattgcgagg ggcaacaatc gcccaatcta cgcctaatgc ggcacgctta cgggtttgga
     7861 tattggcaaa aatagagctg tcccgctcct caatatccat aaataattcg tgttgagcgg
     7921 taatatcgcc attctccgta tcttcaaaaa tcgctttcat tttagcaggc gtaataaaat
     7981 tgctcggatg gtcggagagt acccgacctg tagcggtaat ttcggctaaa tccgtctgca
     8041 attcttgatt tttaatcggg tttaaattcg gtttaatctg cttgttatgc ttctttttgc
     8101 tcataaattt tccttttcag agcttagtta cgctcaatat agtccccctc tttagcaaag
     8161 aggggagtta cattaatgcc gccacttact gcgatactca ccgtcaaaat catctacact
     8221 ctcccactca atcggggcag agctgctgag tgcatttcgc cataacattt ccaacgcatc
     8281 cggaccatca tcgtgatcgg ctttcggaaa atggcgaagc tggctttcaa gcgtagattg
     8341 cgagcggtgc aacaaaatca aaccgttggc aatatgcggt tgcaagctct caatccgtaa
     8401 catcttgtca ctgttcggct tggttgccgt tgccggcacc ggcttaccac gctgggcaga
     8461 acgcttgacc agttccgtct gcaaaaactc ctgaaattgc accgtctcca caaaccagcg
     8521 gtgacagtta tattgcgaat gcagtctaat cacgtcctca ataattaaat ccggcaatcg
     8581 ttttttgatt tgtgcctcaa caacatagag cttgccactc tcacgatgat agccaccgac
     8641 caaaattgca gatgggtcac ggcttgcccc agctttcccc attgaggggt ctaacgcccc
     8701 gaaataaatc aggtcatcag gcagttccgt ccaatactgg atactgttgg caaaaatcgc
     8761 atcatcaccc gagacagggt cattttgata ctcggagtca aatgctgagt gcccatcact
     8821 agcacggatt ttcatcaagt agaggatagg gcgagctaac caagagacta acgctccttt
     8881 atccatttct gctttatgct gttgataaaa gagatcagac atagtgtcat cgtccccttc
     8941 ttccgagagg tatatatttt cccattcgtc ccataacgcc atattgccgg gaaagcgtaa
     9001 aatggcttta aaacgaactc gccgccaacc ttttgtattt aaaatacggc taagcacact
     9061 gtcataatgt agaattgtgc cgacatagat aacatcaaac ttttcacctg cagcaccaag
     9121 ttttaaaact gcttttaaaa cccagttatg caatttatta cgctgtgcag gtgtttctac
     9181 gctttcatca ttctcaatat catcaagtac tactaaatcc ggacgataag ctccgtggcg
     9241 acgaccacgc aatttttgcc cagcccccac agcttcaact ttttgttttt tagccgtcat
     9301 aatgacacct gcacgccaaa ccttgccttg tgcaacctcg gggaaatcaa ttcttaaacg
     9361 agggttagat tcaatttcca ctttaatagc ttcaagcata ctgtatgcct gttctttggt
     9421 gtccatcgca ataatgatgt atctttttaa atcacggact aaacaccaca gcgggaataa
     9481 ttgcgtacaa atcgttgatt ttgcctcgcc acgaggggct gcaatcgctt ggcggactga
     9541 cttagaaaga tcacttaccg aaagcggtaa attctcaaag agataatgat gcaactgtga
     9601 tttatgttca gaccgcacat agtgtggaaa ataagcctgc acaaaatact caaagccgtg
     9661 aacagggtct aaaacctttt gacggcgttc gctaatggct tgtggtttat cgtcccaacc
     9721 ctcaaaactc gcttcgatat tttgttgtaa ctgtcgccgt aattcttcca gttctttttc
     9781 aaattctttg attttcataa attacataca agctgcgata agcaacaacc agccccaacc
     9841 gtctgtaccg ttgagcatta gttttattgc tccgaaaata caagcaagtt ggacaatcca
     9901 acggaaagag taatgcttat gcactacagt ttgttttttc ttagccatta cttaaactcc
     9961 tttttgaatt gcacttctag ctcatctaac atttctaaaa agtcaggcaa taaatgcggt
    10021 ttcttggttt tgacgatatt cgccatcatc tcaatcgcac gcatagcggt tgccattgca
    10081 cttgtttcag gcaatagttt cttgctcgat gccgtcatct tcgcaaacga atcagccaga
    10141 gcagctaatg cctcaacctt agctgcggtt ggcatttcgg tgttttcctc aagctccgtc
    10201 atcaacgtgc ggtacttaat taaaaaaccg gctaacaagc cttgagcgat attctcaata
    10261 ccaccgcttg ccatcgcttg cacatcacga gctttttccc agttatcacc gttcttttca
    10321 gcagccgctt tccaacgccg tgccgtgccg aatgataccc ctgcacgttc tgctgccatt
    10381 tcgaggctta aacggtcaaa cacataggct tgccgaaccg ctttttgtac ttcaacatcg
    10441 tgtgccatac gccctctaca ttccaaattt caggcgtaac aactcaaaac ccacagcaac
    10501 aatgccacca cccaagccac cggcaactac agcatcacgg cggtttttac gtgccatttc
    10561 attcactaaa cgattagtcg cttggatttc acggtgtaag cgttcgattt cttcgttttg
    10621 tttatccacc ttatcgttta gctcatttaa tccacctaaa atcaggtcta atttttccga
    10681 atcggtttgc ttttgttttt tacggctcat tacttatccg cctttttatc aagtttttgg
    10741 tttacttctt tgagcgagtc taaaatatcg tccagctttt ccttaatccc tctattcact
    10801 tcgtgggcaa gctctttgga ttgatatttg ctctccattt ctgcttttag ctcttttacc
    10861 gtttgctcat tgcggttgat acgatcaaat atgaccttgc ctacaaacgc cactaacgga
    10921 gcaactacaa aagagatcag catctgaaac agtttttcat caatcataac gccccctttt
    10981 ggctcgcccg accgggtgtt gattaccttg tgccaccgcc gcacgcacca cgttatgcaa
    11041 ttctgcaagg tcaggacggc ggcgataaaa acgccattgt tgccaacgct gtctgagttt
    11101 tttaattaac ttcattctgg caaacttcct cataggtcag attgtgtgca agcacttggc
    11161 gtttcgtttc cattgtgtcc gcacggctcg ggtaaatcag accgaacgcc gtgcaacccg
    11221 ttgtcgtcac ggaagtaacc gtgttggtgc aactgctcat caatggcact aggctgaata
    11281 cggcgagttt cactaacgtg tttttgctga attttggcat ttttaacctc tgcatttttt
    11341 tgttgaattt cgaccgcttg ttgctgcttt tccgccttta atgtagcggt aagtttccgc
    11401 tcctgcttta attgccagct tttataaaag gcataggcta aaattgtgct tactacacca
    11461 accacacctg caatttgttg agtaatcatt cttcagcctc ccttgagcgt tctttttgtt
    11521 ttaacgcact taccacccct tttgtggctg ggctacccaa tacacaaata ccagcataag
    11581 caaaccacca gtcggaaata aacggcttat cgtaaaggta ggcgtgaatt aaaatgcccg
    11641 ataacactaa ccagctcata aactgaataa agccagtggt gctggctgtg ccgtctttat
    11701 tggtaattaa atcaagtaaa aattgcttca cgattattcc tccaccgtgt ctttggcaaa
    11761 ataacgcaaa ttacccgcca ttcggttaat ccaacctcta ccaaaacggt caaagtgttt
    11821 gagtttggtg taaaactcaa ggcgttctgc ctgaaataac aagcccacat ccgcaaccga
    11881 ataacgctca atcgcagcca aagtcacttt accgataatg ccgtcatcag ccacacctac
    11941 cgctcgctgt aacattcggc tagcattgcc cgaaccgtgg tttacacacg catctaaaaa
    12001 ctgaaaagcc aactcaggcg ggaactgctc acagttataa cgttgccaaa aggctttgag
    12061 gtaaatagtt ttcgcatccg cacggctcat tgcacgcata gagccggtat agccattcgc
    12121 acgggcagta tggatagtga taccccaatt cgtttcgcca ccggggtctt tcgggtcgtt
    12181 cacataaccg ccctcgtgtc cgataagacg gtcaaatgca agattaaagt cagcaatagt
    12241 tgccttgttt ttgataggtt cagtcataaa aaaagcctct ttaaattcaa tgatgattta
    12301 aagagacttt aatgatttag agcaacgggg agcaggcgaa aggattccac acttaaaaca
    12361 aggtagcttg cgaaacggta tgcttacgct gataatatcg tactgcctcc cacgcaacac
    12421 gatcacacac gctgtattta gggcaaattt gcataatcgc aagcctgcca cttagccctc
    12481 cttgctcggt taattgacaa taatcggcat aaatctgctg attacgcaac attcttaatg
    12541 cggtttcaca acgtggcaaa tagacctcgc aagctcccat ataagcctgt aatttcactg
    12601 catcatcttg ccctaatacc tctttgagct tattaaaata gagttttccg ttagaaaatt
    12661 ggaatgaaaa gccaccaaag gctttgatta gcttttcagt tgccggcaaa ccaatcacgc
    12721 ccacaatttc tttcaccgtt tccggcaaat aatgttcgac actttcaaat tcagacataa
    12781 aaaatcccct tgaataattt ccacgaaagc cattatccaa gggggttatt caaatctgta
    12841 atacttactt aaaaattttt ttgcaaattt tttaaaagct cagcccattt tgtgtacggc
    12901 atatctgatc tagctgcggt tgatgacagt tctgtctccc accgcattac tattctttgc
    12961 ccgaccccta atatgttagc tacctctgtt tgtgtgaggt tatggtgctt tcttagcatt
    13021 tttaagttgc ttggtgtgta tccaaactct gggttttcaa tcattcacct ccctccatta
    13081 aactagctat taaatacagc aacaacgata gggtttctaa taatgctctc gcctttatct
    13141 tctccgcctc ttgcactatc gcctttaact aggataaagt tttgcccaca gtatgactgg
    13201 aaacctttaa attcaacatc aaattttttg caagcatcac gacctagatt tttaatagca
    13261 tttaacaaac ctgcttcatc tgcattttgg attcccattg tacaaatgcc gtctaattct
    13321 tcatctgttt tttcaccatc gacccaaaca aaagaatttc ctaattcatc gccaacttga
    13381 tgcacatctg tagttgctct taaaccataa acagcgaagc agtcttcatc ggcatcgttt
    13441 agtaaagcat ttaaaagttt ttggtaatct gtcattttaa tttctcctgt ttggctatgg
    13501 gtgcattata gtaatataat tacctattgc aagtgatttt tcgttttttt cttaaatttt
    13561 tttatgtaaa taaaaaagac cgcttgaaag cggtctaaat ttattttttc ttggttgcag
    13621 aaatggattt tgcaatggta aaggcagttt cccaagcctg ttgattatct gtaacctcaa
    13681 ttttacggca agcaatcgcc tcgttgttaa agtcagtcat atcaaccttg tccgaattaa
    13741 caagcaaaat aacacggcgt aaagcacaag cagcaatagg attgtgatcg tgcccaaacg
    13801 aaccagtttc cattccgtaa gctacatttc ttaactgttg ataatcacca cttacaagag
    13861 catttgctgt ttcttgttca gctttttctg tcattgctat tgctggtaac gacaataagc
    13921 ataaggttgt taatagcagt tctttcattt taaatacctt taaaagttga ttaaaaaaca
    13981 cctaaaatct accacaaaaa agcccactat ttaaagtggg ctatgcaata aatttattat
    14041 ttaacactca ttccgtgcct atggttataa ctcgccaata tctgcactaa tttatgcaac
    14101 tgatcatctg tcagccattg cacccgctca ataccaaacg agcgtttggc aatggcgtgg
    14161 gcgtaatccc acggcttttg gctttgatac agcaacgccc cgattttatt tatcaaccct
    14221 tgccggtgag gggcggtttt acttgctgtt gggcgtttac cgtatttttt cgacttcacc
    14281 acaaaccctt tagctttcat tgtttgcagt acggtcatta attcgctatc ggtcatcatt
    14341 gagcagcttg gtttatccac cgcctctaac aacagcattt tataggcgtt atcatccagt
    14401 tttagctcat ttttgccgat atggattttc tgtatcatct gctttctagt ttgcggttgc
    14461 attttgcttt tcctcctgcc atttcagcca aatttgataa ctttcggtgt tcttaacggc
    14521 ttccagttgc cccatttgct tcattcgttc cacatacatc acggcatcgt gtttcttttg
    14581 gtctgcttgc cgttgctgtt gctcaaggct actcactgct ttcgcttcac ttcttactac
    14641 tgcaaattgt ggcttcacgc tttcatatac tttcttcaag taattatgat ttgccagcgg
    14701 ctcacatctg ccagtttcac gccgattccg tcttacttgt tccaccgtat ccattaaggc
    14761 tttttctaac acattagagg gagggtaaag ctccaataaa ctctgcacaa ttttcaaggc
    14821 tcggctattg gcaaggctgt ttttctgtgg cttaaataac cctaaatacg ccaccaccgc
    14881 acgcccacaa ttcccaccga tattgactat cgtatttaac aactcacgcc ctgcatcatc
    14941 ttcaatcaac gcctcaaacg agatgtcgga atggcaaatc gggcatttgc atagtttcat
    15001 cattctgttc cttcttctat tgtttcgaga tcaacccaat aagttcgact atcagggtat
    15061 gggttatgcc aagttgctgg agttagctcg caaataacta attgttgtaa cacaatctct
    15121 cccgatttct ttttacataa ccgatattca ggaatttttt gatattccat tttatgctcc
    15181 tataaaacac attattcagc ccacttaaat aggggttaaa tgggctgtaa atgggtttta
    15241 ttacttcccc tgtattgcta aaaactcact ttctttaatc tcaaccaatc cctctgcaat
    15301 ctcaggaaat ggttcatcat ttttatcaat aggcattggc atttcaacca gtaaggtgtt
    15361 ttcacaaatg cctgctacgg ctaaataact tctacctgat gttggggaat aactaaaatc
    15421 acttacaaaa taaagcatat tcaaacgttt taagatatag ggcgaaaaat cacgatgttt
    15481 ctcataaatt tcattaattg ctttaaaatc ttctgcaagt tctttccctt tttcgtaacg
    15541 tttatcaggt ttgacgaggt aaaactcatc attaaattta ccgaccttga accctttaac
    15601 atctaaaact tggctaatat tggttgcttt acaagcaatc cctataacta ttctatgaaa
    15661 ccagcctgac ctactaatta atccatcatt gaaaggatat ttttctaaaa tagcattgag
    15721 cttatccttg cgttcttctt tagatacttg ataatctgca taaatgcttt ttagtggctc
    15781 ttgggttata tcccctttaa aaaatctaaa tttcattctt aactccttac tggggtggtt
    15841 aatgtttccg atttgccgtt aataacttca aatgcacctt tggcaacaaa tcctgcaccg
    15901 tgccgagata aacaccactg cgaataaagt cactctgttt taaatatttt tgtgcctgaa
    15961 taatctgcat taacgcctcg tttaattggc tgttaagttc gtttttctct tgttcggtca
    16021 tcatctcctc ctaaaacggt ttcttatgca tacgtttaca aaataccgct cgttgccccg
    16081 cccagtctca gttagcggtc ttttttgctg ataattttgc aactttccaa tggtcttggg
    16141 cttctgcata attgccttta cgttcggctt ccgctgcttt ctcggcataa tgtttgtagc
    16201 ggtcaaactc ggtcgccttt tgttctgttt tcattttttc ctcctgtggt taaaacactt
    16261 tataaatgcc cttctatctt gtccccctct ttgaaaaaga ggggctaggg gagattttta
    16321 a
//
LOCUS       DANLXF010000036        16226 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.036, whole genome shotgun sequence.
ACCESSION   DANLXF010000036 DANLXF010000000
VERSION     DANLXF010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16226
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.036"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(274..1017)
                     /locus_tag="PXD66_000705"
     CDS             complement(274..1017)
                     /locus_tag="PXD66_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319031.1"
                     /translation="MLNKHINKIINSSFSLAFFTLTSFATAYCYGWGQAIFHGYPWWH
                     VEIGNASMARSLAYVFGTSVILFLCYALGYSLLNKVFQLHYFRNLGWLRVIVLVSVFT
                     VPVILTFYLFTGIVPIYISFIYIFVSGVSVFFFRKRWNNTKFDLDFRKMLLGGSFALF
                     NIFIFLYFSLLSLHIGYLRAGLRTTYDYIEIENQKYYILSTNSDNGYIMGTKIRGNQS
                     FLFFNRDTHQYYHVHIEQCTDSRLCKVIV"
     gene            complement(1188..1526)
                     /locus_tag="PXD66_000706"
     CDS             complement(1188..1526)
                     /locus_tag="PXD66_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL6319032.1"
                     /translation="MKLITTIIQPSKLDQVQDALSEIGIQGMTITEVKGFGRQYGHTE
                     QELSQMSRIDFRPKIKVEILVSNYQTDEVIDAILNASYSGQVGDGKIYVANIEQVIRT
                     RTGETDDEAV"
     gene            1785..3683
                     /gene="dnaK"
                     /locus_tag="PXD66_000707"
     CDS             1785..3683
                     /gene="dnaK"
                     /locus_tag="PXD66_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDL6319033.1"
                     /translation="MGKIIGIDLGTTNSCVAVMDGDKPRVIENAEGARTTPSIIAYTD
                     KETLVGQPAKRQAITNPKNTLFAIKRLIGRRFTDAEVQRDIEIMPFEISKADNGDAWV
                     TVKGDKLAPPQISAEILKKMKKTAEDFLGEPVTEAVITVPAYFNDAQRQATKDAGRIA
                     GLDVKRIINEPTAAALAYGLDSKKENQTIAVYDLGGGTFDISIIEIDNFDGEQTFEVR
                     ATNGDTHLGGEDFDNRVINYLVEEFQKEQGVDLRNDPMAMQRVKEAAEKAKIELSSAQ
                     ETEVNLPYITADATGPKHLNIKVTRAKLESLVEDLVNRSLEPLKTALADAGLSVGDIN
                     DVILVGGQTRMPLVQKKVADFFGKEPRKDVNPDEAVAIGAAVQGGVLSGSVTDVLLLD
                     VTPLSLGIETMGGVMTTLIEKNTTIPTKKSQVFSTAEDNQSAVTIHVLQGERKRAADN
                     KSLGQFNLEGINPAPRGMPQIEVTFDIDANGIINVSAKDKNTGKEQQIKIQASSGLSD
                     AEVEQMVRDAEANAEADKKFEELVQARNQADGIAHATRKQITEAGDALNADDKAKIEA
                     AISELETAAKGEDKAEIEAKIEALIKASEPLMQAAQAKAQGGEQPQQSQKDDGVVDAE
                     FEEVKDNK"
     gene            3825..4127
                     /locus_tag="PXD66_000708"
     CDS             3825..4127
                     /locus_tag="PXD66_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319034.1"
                     /translation="MLKLYPTTENVQPPFYKIYDHKSGETVIATETENSNTAILMDDF
                     VWFEQIPTTEALLADTWSVIENRSKQGLLKRAYTKDFEKHLADGKVEIYIAVNPHC"
     gene            4350..5489
                     /gene="dnaJ"
                     /locus_tag="PXD66_000709"
     CDS             4350..5489
                     /gene="dnaJ"
                     /locus_tag="PXD66_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015939959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDL6319035.1"
                     /translation="MAKKDYYEVLGLSKGASEKDIKRAYKRLAAKHHPDKNQGSKESE
                     EKFKEITEAYDVLTDSEKKAMYDQYGHAAFEQGGAGAGGFGGFGGGGFGGFEDIFSEM
                     FGGGFGGGGRRQRVVRGDDLRYDIEITLEEAVKGCKKDIRIQTLAECDTCHGTGAEAG
                     SKVETCSHCHGSGRVRRQQGFFVTEAVCPSCHGTGKRIEKPCRSCHGDGRVQKAKNLS
                     VTIPAGVDTGNQLRLSREGAAGENGAPAGDLYVVIHVKEHDIFERDGSNLYCEVPISF
                     TLAALGGEIEVPTLDGKLKLKIPAETQTGKLFRVRGKGVTSPRGGYAGDLICKVVVET
                     PVNLTEEQKALLRQFEESLEGQGKHRPKHEGFFDGVKKFFDNLGK"
     gene            5777..7285
                     /locus_tag="PXD66_000710"
     CDS             5777..7285
                     /locus_tag="PXD66_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HDL6319036.1"
                     /translation="MSKCPVTHLTMNNGAPVVDNQNSLTAGPRGPLLAQDLWLNEKLA
                     NFVREVIPERRMHAKGSGAFGTFTVTNDITKYTRAAIFSQVGKKTEMFARFTTVAGER
                     GAADAERDIRGFALKFYTEEGNWDMVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNMRS
                     ATNNWDFWTLLPEAFHQVTIVMSERGIPASYRHMHGYGSHTYSFINAANERFWVKFHF
                     HTQQGIKNLTNEEAAAIIANDRESHQRDLYEAIERGDFPKWKMFVQIMPETDADKVPY
                     HPFDLTKVWPKSDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGISVSPDRMLQ
                     ARLFNYADAQRYRLGVNHHQIPVNAPRCPVHSNARDGQGRVDGNYGSTIHYEPNSFGK
                     WQEQAQYAEPPLKINGDAAHWNYREDDADYFSQPRVLFNMMNDEQKQALFNNTAAGMG
                     DALDFIKYRHIRNCYACDPAYGEGVAKALGMTVEDAIAARATDPALGQPGLL"
     gene            complement(7350..7571)
                     /locus_tag="PXD66_000711"
     CDS             complement(7350..7571)
                     /locus_tag="PXD66_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HDL6319037.1"
                     /translation="MNVRLPASGLVCPFPLVEAKAAMAKLNKGDSLTIEFDCTQATEA
                     IPNWAEEEGYEVTDYQQLGDALWEITVVK"
     gene            complement(7600..8109)
                     /locus_tag="PXD66_000712"
     CDS             complement(7600..8109)
                     /locus_tag="PXD66_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HDL6319038.1"
                     /translation="MLFTGLLCGFLLGFVMQRGRFCITGAFRDLYVTKNSRMFVALLI
                     AITVQSIGIWLLYEAGSFSSPAEDLPLLAVIIGAFLFGIGIIYAGGCATGTWYRAGEG
                     LIGSWVALVIYGLFSASMRTGVLAPLNQELKSNVIQHRTIYETFGISPWVLVFILSVI
                     TLALSAHSI"
     gene            complement(8216..9550)
                     /locus_tag="PXD66_000713"
     CDS             complement(8216..9550)
                     /locus_tag="PXD66_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDL6319039.1"
                     /translation="MQLYSYICLLFGLAMFISFFTRKLNENIQTTIAITASALVGSLL
                     ILTFGSLGWFNVDKLAISIFEQLDFKSFLLNGMLGFLLFAGSLGIKLPLMKEQRREIA
                     VYALFSTLVSTFLIGALIYGVARLLGLELGFVYCLLFGSLISPTDPIAVLAIIKSLKA
                     PKRLSMHVEGESLFNDGIGLVIFTTIFAVAFGGQEPNLSGITGLFLQEAVGGIVFGLL
                     TGFVAHKFISATDDGSLEILITLTIPTVGFVIANYLHVSGALTMVVAGIMIGNWTRRS
                     GFSEQSQKYLDHFWEMIDHSLNYLLFLLIGLSTLLVDFTFIGGVLMLAAIPICLLARY
                     ISLWIPYQVLKRFRTYNPYTLKIMTWGGLRGGLALAMALSVPAGAAVVSSSEGNLDVR
                     DLILLMTYAVVTFSILVQGSTVEPMIKKSKLVDPRKVALNKLGAPDDVIPHI"
     gene            complement(9661..10809)
                     /gene="dapE"
                     /locus_tag="PXD66_000714"
     CDS             complement(9661..10809)
                     /gene="dapE"
                     /locus_tag="PXD66_000714"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HDL6319040.1"
                     /translation="MKNEIISLAQNLIRRDSISPQDKGCQQEIAQRLEKLGFNIEWLP
                     FGDTLNLWATHGSENGKTEPCIVFAGHTDVVPVGDESQWAYPPFSAEIVDGMLYGRGA
                     ADMKGSLSALVVAAETFVQHHPNHKGKVALLITSDEEAAAKDGTVKVVETLMARNEAV
                     HYAVVGEPSSSKVFGDVIKNGRRGSITADLYIEGIQGHVAYPHLAENPVHTSLGFLTE
                     LTTYQWDNGNEFFPPTSLQIANIKAGTGSNNVIPGELYVQFNLRYCTEVTDEIIKAKV
                     AEMLEKHGLKYRISWNLSGKPFLAGNGKLVEAALQAVENVTKITPRLDTGGGTSDGRF
                     IALMGAEVVEFGPLNATIHKVNECVSADDLAHCGEIYYQILEKLLATA"
     gene            complement(10939..11286)
                     /locus_tag="PXD66_000715"
     CDS             complement(10939..11286)
                     /locus_tag="PXD66_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HDL6319041.1"
                     /translation="MTTLVYGIKNCDTVKKALKWLEDHNQNPQLHDYRVDGLNADWLV
                     EMEAKFGWENLVNKRSTTWRNLDEEIKNNLNRESALKVLTEQPTLIKRPIVIKEDIAL
                     IGFNEKEYVAAFN"
     gene            complement(11286..12143)
                     /gene="folP"
                     /locus_tag="PXD66_000716"
     CDS             complement(11286..12143)
                     /gene="folP"
                     /locus_tag="PXD66_000716"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDL6319042.1"
                     /translation="MQIHFKNFQKNDRLLDLTTPQIMGILNFTPDSFSDSGKFFSLDK
                     ALFQAEKMLADGASIIDIGGESTRPNAAIVSLEQELERVVPVVEAIRSRFDCLISVDS
                     SKAEVFKESAKVGMDIINDIRALTEPNALAAAVELGLPVCLMHMQGNPQTMQQNPHYD
                     DVLEDVADFLNQRIFECITAGIPKEHIILDPGFGFGKSVKHNYQLLKHLKAFVDSGFP
                     VLAGLSRKSMIGAVLDKPVEQRLLGSVVGALLAAQNGAKILRVHDVAETADALKIWQA
                     MLESDNEEK"
     gene            complement(12252..13214)
                     /gene="rluC"
                     /locus_tag="PXD66_000717"
     CDS             complement(12252..13214)
                     /gene="rluC"
                     /locus_tag="PXD66_000717"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HDL6319043.1"
                     /translation="MTTEKVISAGVQFFTISEDEAGQRLDNFLLAKLKGVPKSLIYRI
                     VRKGEVRVNKGRNKPEYKLQPDDLVRVPPVRVAEKNEAPISTKLNKVAELESQILYED
                     ESMLVINKPSGIAVHGGSGLSFGVIEALRALRPQARFLELVHRIDRDTSGILLVAKKR
                     SALRSLHEQLREKVVQKDYLALVRGQWQAHTKVIKAPLLKNELASGERIVRVSEEGKP
                     SETRFSIEERYANATLVKASPITGRTHQIRVHTQYAGHPIALDDKYGDAEFDSKMKEV
                     GLQRLFLHAHAIRFEHPKTGEEMVITAPLDKTLKGVLAKLRANY"
     gene            13323..14225
                     /locus_tag="PXD66_000718"
     CDS             13323..14225
                     /locus_tag="PXD66_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HDL6319044.1"
                     /translation="MTEKVTETAPNIDVVAETEKVIHKVSNMNIETMLNEWIIPYGTK
                     ILLAIAIFVVGKFLARLISRLLGKAALKSTKDEMLQSFIVSISYFLLLLIVVIASLSQ
                     IGINTSSLVALIGAAGLAIGLSLQNSLQNFAAGVMLLIFKPFRKGDLIETGGMTGVVE
                     QMGLLVLELRTGDNKTVLLPNGKVFSDSIINYSNNPTRRIDFTFDISYESNLKEAKEI
                     IQRILDANTYVLKDPAPVVAVGALAPHSVQLVVRPWVQTPNYWAAYWEIIEQVKLAFG
                     EAGISIPYNQMELHIKSGSLENKA"
     gene            14323..14820
                     /gene="rraA"
                     /locus_tag="PXD66_000719"
     CDS             14323..14820
                     /gene="rraA"
                     /locus_tag="PXD66_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HDL6319045.1"
                     /translation="MRIDTSALCDLYSDQVDVVEPIFSSFGGASSFFGKITTVKCFES
                     NGLIAEVLEEEGEGRVLLVDGGGAVRRALIDAELAQLAVDNGWEGIIVYGAVRQLDIL
                     ERLDIGIHALAPIPVGADNTDIGEVDTPVNFGGVTFFPEDYVYADLTGIILSQELLDL
                     NDISE"
     gene            complement(14868..16211)
                     /locus_tag="PXD66_000720"
     CDS             complement(14868..16211)
                     /locus_tag="PXD66_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005701931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDL6319046.1"
                     /translation="MKQLFIFAFTLVLSACSLVVYKPVETIKQVNVERGYRQKNVIAE
                     SAKDGNIVVIMFSGGGTRAAALGYGVLEEFKKVRVKETNKGNTLLENIDIAFGVSGGS
                     ILATYFALEGKDIIPKFADRFLKANFQKDIITEVFSLSNLPRLSSPQYGRGDLLQERL
                     NFALYHGKKFGDLVKNRKGPFAVISATDMNIGQKLTFTQETFDGLCLNLNDLEISRAV
                     AASSSVPLIFAPLTLNNNGGNCHFSMPQEFSAREKAIDGLITKNIEETNHILSLYQDS
                     KERPFIHLVDGGLTDNLGLASVLEISDVMGIEEMYQYIHQLRVKNIVVINVNAQNEVS
                     SEIDKTANVPGVADVVNTIVNVPIDRTTQINLQRYRQFTDGWNEYANKQPSKIEMHFV
                     SLGLKELPDGDLKKDVLNIATSFYLPEEDVDKLREAARVLLQQSQEYNAALKTLQ"
BASE COUNT         4946 a         3474 c         3266 g         4540 t
ORIGIN      
        1 gtataaaaaa acccggtaaa taccgggctt ttatttcaat tggttatcca acttgcgaga
       61 gcaattaccg caataaaaat aactaaccaa ataagttgta attttacgct tttgcggttg
      121 atctcctggt tgtgtaatcg gcgctgttct aacttttgcc gatatatcgc taaatcggct
      181 tctttaaacg agaagccaca atgtatgcaa tgttctaaac tgtcgctaat acgctttcga
      241 cactccggac aacggtgtaa tgccataatt acctcaaact attactttgc ataatcggct
      301 atccgtacat tgttcaatat gaacatggta gtattgatga gtatcacgat tgaaaaacaa
      361 gaaagattga ttacctctta tttttgttcc cataatgtag ccgttatcac tgttggtgga
      421 taatatgtag tatttttgat tctctatttc gatgtaatca taggtagttc ttagtccggc
      481 acgcaaataa ccaatatgca atgagagcaa actgaaatag agaaaaataa agatgttaaa
      541 cagggcaaaa cttcctccta atagcatctt tctaaagtcc agatcaaact tggtattatt
      601 ccatctcttg cggaagaaga atacgctcac accacttaca aaaatataga taaaagagat
      661 ataaatgggc actatcccgg taaataagta aaaagtaaga ataacaggca ctgtaaacac
      721 agaaaccaac acaatcaccc ttaaccaacc tagatttcgg aaataatgca gctgaaaaac
      781 tttatttagt aaactataac ctagtgcata acacaagaat aagatgacgg aagtaccgaa
      841 tacataagcc aaacttcttg ccatactggc attgccgatt tcaacatgcc accaagggta
      901 gccatgaaat atggcttgtc cccaaccgta acagtaagcg gtagcaaaac tagttaatgt
      961 aaaaaatgca agagaaaatg aagaattgat aattttatta atgtgtttat ttagcataac
     1021 aaagaatgtc tatttccatt aaaacctaac tatttttgat tactataata acccgtatta
     1081 cacaaaaaac aaacatttaa ttcacaccaa attagaaaat ttgctttagt aaggttgaaa
     1141 atataactct aaaacacgat aataaaaagc ttctctgcta gcaatggcta gactgcttca
     1201 tcgtcagttt cgccggtacg ggtgcggata acttgttcaa tattcgctac ataaattttg
     1261 ccatcaccca cttgcccaga ataggatgca ttgagaatgg catcgatcac ttcatcggtt
     1321 tggtaattag aaaccaatat ctccacctta attttaggac gaaagtcaat gcggctcatt
     1381 tggctaagct cttgttcggt atgcccatat tggcgtccaa agccttttac ttcggtaata
     1441 gtcattcctt gaataccgat ttctgaaagt gcatcttgca cttggtctaa ttttgaaggt
     1501 tggataatgg tagtaatcag tttcatatta tatccttctc tgtttgagtt gccgtaagta
     1561 taaaaagcta ttgaggggta tgcaaggtag gaggtatttt ctccgtgaaa tattctgcca
     1621 aatctccccc acatacaagc ggttactttt tccaaaaatt ttgcaatttt tttatttttg
     1681 cccttgaaca gtgcattttc gtccctattt atgatttcgt ctgaacggga aatgcaatcc
     1741 cgtttcttat tcaaactgaa tttattatta taggaatcat aaaaatgggt aaaatcattg
     1801 gtattgactt aggtacaaca aattcttgtg ttgcggtaat ggacggcgac aaacctcgtg
     1861 taattgaaaa tgcggaaggg gcaagaacaa cgccatctat catcgcttac accgataaag
     1921 aaaccttagt tggtcagccg gcaaaacgtc aggcaattac taacccgaaa aatactttat
     1981 ttgcaatcaa gcgtttaatc ggccgtcgtt ttactgatgc tgaagtgcaa cgtgatattg
     2041 aaattatgcc gtttgaaatc agcaaagcag ataacggcga tgcgtgggta accgtaaaag
     2101 gcgataaatt agcaccgccg caaatctctg ctgaaatctt gaaaaaaatg aagaaaaccg
     2161 cagaagattt cttgggcgaa ccggtaaccg aagcggttat tactgtgcct gcatacttta
     2221 acgatgcaca acgtcaggca actaaagatg ccggtcgtat tgcaggttta gatgtaaaac
     2281 gtatcatcaa cgaaccaaca gcggcagcgc ttgcttatgg tttagattct aagaaagaga
     2341 accaaacgat tgctgtttac gacttaggtg gtggtacatt cgatatttcg attatcgaaa
     2401 ttgataactt tgacggcgag caaacttttg aagtgcgtgc aaccaacggc gatactcacc
     2461 taggtggtga agacttcgat aaccgtgtaa tcaactactt agtggaagaa ttccaaaaag
     2521 aacaaggcgt tgatttacgc aacgacccaa tggcaatgca acgtgtgaaa gaagcagcgg
     2581 aaaaagcgaa aatcgaactt tcttctgcac aagaaactga agtgaacctt ccatacatca
     2641 cggcagatgc aaccggcccg aaacacttaa acattaaagt aacccgtgcg aaattggaat
     2701 ctttagttga agatttagta aaccgttcgt tagagccgtt aaaaaccgct ttagctgatg
     2761 ccggtttatc agtaggcgac atcaacgatg tgattttagt cggtggtcaa acccgtatgc
     2821 cgttagtaca gaaaaaagtg gcagacttct ttggcaaaga accgcgtaaa gacgttaacc
     2881 cggacgaagc tgtggcaatc ggtgcggcgg ttcaaggcgg tgtgttaagt ggttcggtaa
     2941 cagacgtatt gttattagac gttactccgc tttcacttgg tatcgaaaca atgggtggtg
     3001 taatgactac cttaattgag aaaaacacta cgattccaac caagaaatca caagtgttct
     3061 caacagcgga agataaccaa tctgcggtaa ctatccacgt gttacaaggt gagcgtaaac
     3121 gtgcagcgga taataaatct ttaggtcaat tcaaccttga aggtattaac cctgctccac
     3181 gcggtatgcc acaaattgaa gtaacctttg atattgatgc taacggtatt atcaatgtct
     3241 ctgcaaaaga taaaaacact ggcaaagagc aacaaatcaa aattcaagca tcttcaggct
     3301 taagcgatgc tgaagttgaa caaatggtgc gtgatgcaga agcgaatgcg gaagcagaca
     3361 aaaaatttga agagttagtt caagctcgta accaagcaga cggtattgct cacgcaaccc
     3421 gtaagcaaat taccgaagca ggcgatgcgt taaacgcaga cgataaagcg aagatcgaag
     3481 cggctatttc tgagttagaa accgcagcta aaggcgaaga taaagccgag atcgaagcga
     3541 aaatcgaagc gttaatcaaa gcttcagaac cgttaatgca agcagcacaa gcaaaagcac
     3601 aaggcggcga gcaaccacaa caatctcaaa aagatgacgg cgtggtcgat gcggaatttg
     3661 aagaagtgaa agataataaa taatcttgcg taacttgtag gggcgaaata tctttcgccc
     3721 cttttgtgta gatatatacc acctagcacg agccgtgcta ggttacacat aattcagccc
     3781 ctttggggct gtgcaagcgg tgcttttcaa aggaaatttt acaaatgcta aaactctacc
     3841 ctacaacgga aaatgtacag ccaccgtttt ataaaatata cgatcataaa agtggcgaaa
     3901 cggtcattgc cacagaaacg gaaaacagca ataccgcgat tttaatggac gattttgttt
     3961 ggtttgagca aattccaact accgaagctc ttttggcaga cacttggtct gtgattgaaa
     4021 accgttccaa acaaggtttg ctcaaacgag cttacaccaa agattttgaa aaacatttag
     4081 cagacggcaa agtagaaatt tatattgcgg tgaatccgca ttgttaggct tttgatagcg
     4141 caagcgtcca cgcttgtgtc atcttgagcc atcttggcta gatcccattt aattatataa
     4201 aactctctcc ccttgtggga gagagaccga aaaatatttc gttcagaaat atttttcaga
     4261 gagaggggga attaaccatc ctacccctct cccacaaggg gcgagggcaa gcggtcaaaa
     4321 acacagaaaa atttacaaac ggaacaacaa tggcaaagaa agattattac gaagtccttg
     4381 gactgtctaa aggggcaagc gagaaagaca tcaaacgagc atataaacgt ttggcggcaa
     4441 agcatcaccc ggataaaaac caaggcagta aagagtcgga agagaaattt aaagagatta
     4501 ccgaagccta tgacgtgctg accgacagcg agaaaaaagc gatgtacgac caatacggtc
     4561 acgccgcctt tgaacaaggc ggtgccggtg caggcggatt cggtggcttt ggcggcggtg
     4621 gtttcggtgg ttttgaagac atcttcagcg aaatgtttgg cggtggcttt ggtggcggtg
     4681 gacgtcgcca acgtgtggta cgtggcgatg atttacgtta cgacatcgag atcacccttg
     4741 aagaagctgt gaaaggctgt aaaaaagata ttcgcattca aaccttagct gaatgtgata
     4801 cttgtcatgg tacaggggcg gaagcaggga gcaaagtgga aacctgttcg cattgtcacg
     4861 gctccggcag agttcgccgt cagcaaggtt tctttgtgac tgaggcagtt tgtccaagtt
     4921 gtcacggtac aggtaaacgc attgaaaaac cgtgccgttc ttgccacggc gatggacgtg
     4981 tacagaaagc gaaaaacctc tctgttacca ttccggcagg tgtggatacc ggtaatcaac
     5041 ttcgcttatc gagagaggga gctgcaggcg aaaatggcgc accggcaggc gatttatatg
     5101 tggtgattca cgttaaagaa cacgatattt ttgagcgtga cggttcgaat ttatactgtg
     5161 aagtgccgat tagctttacc cttgcagctt taggcggtga aattgaagtg ccaactttag
     5221 acggcaaatt aaaactcaaa attccggcag aaactcaaac cggtaaacta ttccgtgtac
     5281 gaggcaaagg ggtaacaagc cctcgtggtg gctacgcagg ggatttaatc tgcaaagtgg
     5341 tggtcgaaac gcctgtgaat ttaaccgaag aacagaaagc actattacga caatttgaag
     5401 agagtttaga aggacaaggt aaacatcgtc cgaaacacga aggtttcttt gatggtgtga
     5461 agaaattctt tgataactta ggcaaataaa atcataaaaa gtacatattt cttaaatatg
     5521 tactttttta ttatctattc tttgttgaaa tctacttctc ggccgttaat ttttacacct
     5581 acatactgca aaaaatagtg gaaaaatgac cgcttatcat ctttctcaga atacttgaca
     5641 attaaaatca actatttaaa attaatattt aatagtcatt aattatcaaa ttgatagatt
     5701 gagaaaaaat ctacaacacc tataattctc ttcggattaa ttacctatct tatttctaat
     5761 ttatgaggat tctattatgt ctaaatgtcc tgttacacat ttgacaatga acaacggtgc
     5821 acctgttgtt gataaccaaa acagcttaac agcgggtcca cgtggtccat tattagcgca
     5881 agatttatgg ttaaatgaaa aattagcgaa cttcgtgcgt gaagtcattc ctgagcgtcg
     5941 tatgcacgct aaaggttccg gtgcgttcgg tactttcact gtaactaacg acatcactaa
     6001 atacactcgt gcggcaattt ttagccaagt cggtaaaaaa actgaaatgt ttgctcgttt
     6061 caccaccgtt gcaggtgaac gtggtgcggc agatgctgag cgtgatattc gtggtttcgc
     6121 attaaaattc tataccgaag aaggtaactg ggatatggtg ggtaacaaca cccctgtgtt
     6181 cttcttacgt gacccacgta aattcccgga tttaaacaaa gcggttaaac gtgacccacg
     6241 cacgaatatg cgttctgcaa ctaacaactg ggatttctgg acattattac cagaagcatt
     6301 tcaccaagtg actattgtaa tgagtgaacg tggtattccg gcttcatatc gtcatatgca
     6361 cggttatggc tcacatactt atagcttcat caatgcagct aatgagcgtt tctgggtgaa
     6421 attccatttc catacacaac aaggcattaa aaacttaact aacgaagaag cagccgctat
     6481 tatcgctaac gatcgtgaaa gtcaccaacg tgatttatac gaagcgattg agcgtggcga
     6541 tttcccgaaa tggaaaatgt ttgttcaaat tatgcctgaa actgatgcag acaaagtacc
     6601 ataccacccg ttcgatttaa ctaaggtatg gccgaaatca gactatccgt taattgaagt
     6661 gggtgagttt gaattaaatc gtaaccctga aaacttcttt gctgatgtgg aacaatctgc
     6721 attcgcacca agtaacttag tgccgggcat cagcgtgtct ccagaccgta tgttacaagc
     6781 tcgtttattc aactatgcgg atgctcaacg ctaccgttta ggtgtgaacc accaccaaat
     6841 ccctgtaaac gctccacgtt gcccggttca tagcaacgct cgtgacggtc aaggtcgtgt
     6901 ggacggtaac tacggtagta ccattcacta cgaaccaaac agcttcggca aatggcaaga
     6961 acaagctcaa tatgcagagc caccactgaa aattaatggt gatgcagccc actggaacta
     7021 tcgtgaagac gatgctgact acttcagcca accacgtgta ttgttcaata tgatgaatga
     7081 tgaacaaaaa caagcattat tcaataatac tgctgcaggt atgggcgatg cattagactt
     7141 cattaaatac cgtcatattc gtaactgcta tgcctgtgat ccggcatatg gtgaaggtgt
     7201 ggcaaaagca ttaggtatga cggttgaaga tgctattgct gcaagagcaa cagacccagc
     7261 attaggtcaa ccgggtttac tctaatcctt agtaaataca aaactcaaag caaaagctct
     7321 acaaaaaagt agagcttttt tattgctaat tacttaacca ccgtaatttc ccacaaggca
     7381 tcaccaagtt gttggtaatc ggtaacttca tagccttcct cttccgccca atttggaatg
     7441 gcttcggttg cttgagtaca gtcaaattca atcgttaagc tatcgccttt attcaatttt
     7501 gccatcgccg ctttggcttc gactaatgga aacgggcaaa caagccctga ggcaggtaaa
     7561 cgtacgttca tctttattac tccttaaaaa attaaccttt taaatgctgt gggcggataa
     7621 tgccaatgta attacactca ggataaacac taacacccaa ggggaaatac caaatgtttc
     7681 ataaatcgtg cggtgttgaa ttacattgct ttttagctct tgattaagtg gggctaacac
     7741 accggttcgc atagaagcag aaaacagccc ataaataacc agtgcaaccc aactgccgat
     7801 taaaccctca ccggcacgat accacgttcc tgtagcacaa ccgcctgcat aaataatgcc
     7861 gataccaaac agaaatgccc caatgatcac tgcgagcaaa ggcaaatctt ccgccggaga
     7921 actaaaagag ccggcttcat acaaaagcca aatcccgatc gattgcaccg taattgcaat
     7981 taaaagtgcc acaaacatac ggctattctt tgttacatag agatcacgaa atgctccggt
     8041 aatacaaaaa cgccctcttt gcatcacaaa cccgagtaga aagccacata ataatcctgt
     8101 gaataacata aaaaatacct ctcaacaaat taacgtgaga ggtatttaaa tataaagccg
     8161 tctagatggc taataatctt tagcaaaatg atatgttatt cagttataaa tgctattaaa
     8221 tatgtggaat tacatcatca ggtgcaccta atttattgag tgccactttt cttggatcta
     8281 ccaacttgga tttttttatc atcggttcaa ccgttgaacc ttgtactaaa atcgagaaag
     8341 tgactaccgc ataagtcatt aacaaaatca aatcacgtac atcaagattg ccttctgagc
     8401 tcgataccac cgcagcccct gccggtacgg aaagtgccat cgcaagggct aatccaccac
     8461 gtaaaccgcc ccaagtcata atttttaagg tgtacgggtt ataagtacgg aaacgtttta
     8521 acacttggta tggaatccac aggctgatat agcgtgcaag caaacaaatt ggaattgctg
     8581 ccaacattaa caccccacca ataaaggtga aatcaactaa cagtgtactt aagccgataa
     8641 gcaggaataa caggtagttt aatgaatggt cgatcatctc ccagaagtga tcaagatatt
     8701 tttggctctg ttcggagaaa ccggaacggc gagtccagtt gccaatcata ataccggcaa
     8761 ctaccattgt taatgcccct gaaacgtgta ggtagtttgc aatcacaaag ccgactgttg
     8821 gaatcgttag cgtaattaaa atttcaaggc ttccgtcatc ggttgcggaa ataaatttat
     8881 gtgccacaaa accggttaat aagccaaaaa cgataccgcc taccgcttct tgcaagaaca
     8941 gtccggtaat accgctaagg tttggttctt gtccgccaaa agcaacggca aagatagtgg
     9001 taaaaatcac caagccgata ccgtcattaa acagagattc gccctccacg tgcatcgaaa
     9061 ggcgttttgg ggctttcagg cttttgataa ttgccaatac ggcaatcggg tcggttggcg
     9121 aaatcagtga gccaaacagc aaacaataca caaagccgag ctctaaccca agtagccttg
     9181 ccacgccata aattaacgca ccgatcaaga aagtagaaac taaggttgaa aataaggcat
     9241 aaactgcaat ttcccgccgt tgctctttca ttaatggtag tttaatccca agtgaaccgg
     9301 caaaaagcaa gaaccccagc attccgttta acaggaagct tttaaaatcg agttgttcaa
     9361 aaatggaaat ggcaagttta tcaacattaa accagccgag agaaccgaag gtaagaataa
     9421 gaagggagcc gacaagggca gaggctgtaa ttgcaatagt ggtttgaata ttttcgttta
     9481 attttcgggt aaaaaacgaa ataaacatcg caagcccaaa cagcaagcag atataagagt
     9541 agagttgcat aacatttacc tataaatata aaaactgccg ctagtgtaaa cgctgtttaa
     9601 cagaatagct acataaaaaa taggcagaat acaatttgat aacattctgc ctatttaggg
     9661 ttatgcagtt gccaataatt tttctaaaat ttgataataa atttcgccac aatgtgccaa
     9721 atcatccgca ctcacacact cattcacttt gtgaatagtg gcgttcagtg gtccaaattc
     9781 cactacctct gcccccatta atgcaataaa acgaccgtcc gaagtaccgc cacctgtgtc
     9841 taaacgaggc gtaatttttg tgacattttc gaccgcttgt aaggcggctt cgaccaattt
     9901 tccattgccg gcaaggaatg gtttgcctga taaattccaa cttattcggt atttcaaacc
     9961 gtgtttttcg agcatttccg ctactttggc tttgatgatt tcatcggtca cttcagtgca
    10021 ataacgtaag ttaaactgca cataaagctc gcccggaatc acattattac tgcctgtgcc
    10081 ggctttgata ttggcaattt gcaaactggt aggcgggaaa aattcgttac cgttatccca
    10141 ctgataagtg gtcagctcgg ttaaaaaacc cagtgacgta tgcaccggat tttccgccaa
    10201 atgtggatag gcaacgtgcc cttgaatgcc ctcgatatag agatctgccg taattgaacc
    10261 acgtctgccg tttttaatca catcgccaaa caccttgcta ctagacggtt cgcccactac
    10321 cgcataatgt accgcttcat tgcgagccat taaggtttca accactttta ccgtaccgtc
    10381 tttggctgct gcctcttcat cggaggtaat caataatgcc actttgccct tatggtttgg
    10441 gtgatgttgc acgaaagttt ctgccgccac aactaacgcc gagagcgagc ctttcatatc
    10501 tgccgcccct cgcccataaa gcattccatc tacaatctca gccgaaaacg gcggataagc
    10561 ccattggctt tcatcgccta ccggcaccac atctgtatgc ccggcaaaca caatgcaagg
    10621 ctctgtttta ccattttccg agccgtgcgt tgcccataaa ttcaaggtgt cgccaaacgg
    10681 caaccattca atattaaagc ccagtttttc aagacgttgt gcaatttctt gctgacagcc
    10741 tttgtcttgt gggctgatgg aatcacggcg gattaggttc tgtgcaaggg aaataatttc
    10801 atttttcata ggttacaagc ggttgttttg ggtgaatttt ttgctatata tcctgtaggg
    10861 gcgggtcctg tgcccgcccg aacaatccta aagactacgg acaggcacag gacctgtccc
    10921 tacaagcagg ggaaaaaatt aattaaacgc agccacatac tctttctcat taaaaccaat
    10981 caaagcaata tcctctttaa tcacaatcgg gcgtttaatt agggtcggct gttcggttag
    11041 cacttttaat gccgactcac ggttaaggtt gtttttaatt tcctcatcaa ggtttcgcca
    11101 agtggtgcta cgtttattaa ctaagttttc ccaaccgaat tttgcttcca tttccactaa
    11161 ccaatcggca tttaaaccgt ccacacggta atcgtgcagt tgtgggtttt gattatgatc
    11221 ttcgagccat ttcaaggctt tttttactgt gtcgcaattt ttaatgccat aaactaaggt
    11281 tgtcatcatt tttcctcatt atctgattcc agcattgcct gccagatttt taacgcatcg
    11341 gcggtttctg ccacatcgtg cacccgtaaa attttcgccc cattttgagc ggctaataac
    11401 gcccctacta cagagccaag tagccgttgt tctaccggtt tatctaacac cgcaccaatc
    11461 attgatttac gggaaagtcc ggcaagcaca gggaagcctg aatccacaaa ggctttaagg
    11521 tgtttcaata attggtaatt gtgtttcacg cttttaccaa agccaaaacc ggggtctaaa
    11581 ataatgtgtt ctttgggaat gcctgcggtg atgcactcga aaatccgttg atttaagaaa
    11641 tctgccacat cttccaacac atcatcatag tgcggatttt gttgcattgt ttgcgggttg
    11701 ccttgcatat gcattaaaca aaccggcaag cccaattcta ctgccgctgc caacgcattc
    11761 ggctcggtta aggctcgaat atcgttaatg atatccatac cgacttttgc cgactctttg
    11821 aatacttctg ctttggagct atcaaccgaa atcaagcaat caaaacggct gcggatcgcc
    11881 tccaccaccg gcacaacacg ctctaattct tgttctaagc tcacaatcgc cgcattaggg
    11941 cgggtggatt caccgccaat atcaataatg cttgccccat cagctaacat tttttccgct
    12001 tggaacaagg ctttatcgag ggaaaaaaac ttgcccgaat cggaaaatga atccggcgtg
    12061 aagtttaaga tccccataat ttgcggtgtg gttaaatcta acaagcggtc atttttttga
    12121 aaatttttga aatggatttg catatttttg cttatatttt gttggtgcaa gcgtccacgc
    12181 ttgtaccact ctatgttgat ttacagcaca agcgtggacg cttgcgctat cttactctct
    12241 cattaagtta tttagtaatt agcccttaat tttgccaata cgccttttaa agttttatcc
    12301 agcggggcgg taatgaccat ttcttcgccg gttttggggt gttcaaaacg aatagcatgg
    12361 gcgtgcagaa ataaacgctg taagcccact tctttcattt tgctgtcaaa ttccgcatca
    12421 ccatatttgt catctaacgc aatcgggtgt ccggcatatt gggtatgcac acggatttgg
    12481 tgggttcttc cggtaatagg cgaagctttc accaaagtgg cattggcata acgctcttca
    12541 atgctgaaac gggtttcgga cggtttaccc tcttcactca cccgtacaat tcgctcaccg
    12601 ctggcaagtt cgttttttag caatggagct ttaatcactt tggtatgagc ctgccattgc
    12661 cctcgcacaa gggcaagata gtctttttgt actacttttt cacgcagttg ttcgtgcaag
    12721 gaacgtaacg ccgaacgctt tttcgccacc agcaaaatgc ctgatgtatc acggtctata
    12781 cggtgtacca attccaagaa acgagcctgt ggacgtaatg cacgcaaggc ttcgatcaca
    12841 ccaaaactta aaccactgcc accgtgtact gcaatgcctg aaggtttgtt aattactagc
    12901 attgactcat cttcatacag aatttggctc tccagctccg ccactttgtt taatttagtc
    12961 gaaattgggg cttcattttt ttccgccaca cgcaccggcg gcacacgcac taaatcatca
    13021 ggctgtaatt tatattcggg tttgtttcgt cctttattca cccgcacctc gccttttcgc
    13081 acaatgcggt aaatcaaact tttcggcacg cctttgagct ttgccaataa aaaattatct
    13141 aaacgctgtc cggcttcatc ttcactgatc gtaaaaaatt gaacgccggc actaattact
    13201 ttttctgttg tcattatctt gtcttttaga gaaaattttg ctctaaattc tagcacaaaa
    13261 ccgcttagcg agtgaggcaa attcgctagg cttatttttc attttaattt taaggaaaca
    13321 atatgactga aaaagtcacc gaaacagctc caaacattga cgtggttgcc gaaaccgaaa
    13381 aagtaatcca caaagtcagt aatatgaata ttgaaactat gctaaatgag tggattatcc
    13441 cttatggcac caaaatttta ctggcgattg cgatttttgt agtcgggaaa tttcttgccc
    13501 gcctaattag ccgtttactc ggtaaagccg cattaaaatc aaccaaagat gaaatgctgc
    13561 aaagttttat tgtctcaatc agctattttt tactgctgtt aattgtggtg attgcctcgc
    13621 tttcacaaat cggcattaac accagctcgc tggtcgcatt aatcggtgct gccggtttag
    13681 ccatcggttt atcattacaa aactcattac aaaactttgc tgccggcgtg atgttgttaa
    13741 tcttcaagcc atttcgcaaa ggggatttaa tcgaaaccgg cggaatgacc ggcgtggttg
    13801 aacaaatggg gcttttagtg ttggaacttc gcaccggcga taacaaaacc gtgttattac
    13861 caaacggcaa agtgttttct gacagcatca tcaactactc taacaacccg acacgccgta
    13921 ttgatttcac ctttgatatt tcttatgaat ctaacttaaa agaggcgaaa gagattatcc
    13981 aacgcatttt agacgccaat acttatgtat taaaagatcc ggcacctgta gtagcagtcg
    14041 gcgctttagc accgcatagt gttcaacttg ttgttcgccc ttgggttcag acaccaaatt
    14101 actgggcagc atattgggaa attatcgaac aagtaaaatt agcctttggt gaagcaggta
    14161 tatctattcc ttacaaccaa atggaattgc atattaaaag cggttcacta gaaaataaag
    14221 cataagaaaa aagcctgtaa gcggtctttt ttcgataaaa ttttgcaaat tttgggcgaa
    14281 ggtttatact ttagctaaat aatctacgaa caggagcata ttatgcgtat tgatacctct
    14341 gcactttgcg atctttattc tgaccaagtt gatgtggttg aaccaatttt ctcaagcttt
    14401 ggcggtgctt cttccttctt tgggaagatt accacggtta aatgctttga aagcaacggt
    14461 ttaattgctg aagttttgga agaagagggt gaagggcgag tgctgttagt cgatggtggc
    14521 ggtgcggttc gtcgtgcctt aattgacgca gagctggctc aacttgctgt agataacggc
    14581 tgggaaggta tcatcgttta tggtgcggtt cgccaattag atattttgga aagattagat
    14641 atcggtattc atgctttagc gccaattcca gtgggagcag ataataccga cattggcgaa
    14701 gtggatacgc ctgtgaattt tggcggtgta accttcttcc ccgaagatta cgtttatgct
    14761 gacttaaccg gcattattct ttcgcaagag ttgttggatt taaacgacat cagcgaataa
    14821 atatttgcaa aaaaatacca aaaaccgacc gcttggtcgg ttttttacta ctgcagtgtt
    14881 tttaatgccg cattatattc ttgagattgc tgcaataaaa cccgagcagc ttcacgtaat
    14941 ttatctacat cctcttccgg caaataaaat gatgttgcaa tatttagcac atcttttttt
    15001 aaatcaccat caggtaattc ttttaaacct aaactgacaa aatgcatttc gatctttgat
    15061 ggctgtttat ttgcatattc attccaacca tctgtaaact ggcgataacg ttgtaaatta
    15121 atttgtgtcg ttctatcaat tggcacatta actattgtat tcactacatc agccaccccc
    15181 ggtacatttg cggtcttatc gatctcgcta gatacttcat tttgcgcatt tacattaatc
    15241 actacaatat ttttcaccct aagctgatga atatactggt acatctcttc aatacccata
    15301 acatcagata tttccagcac actggcaagc cctaaattat ctgttaatcc accatcaact
    15361 aaatggataa aggggcgttc tttactatct tgataaaggc tgagtatatg attcgtttct
    15421 tcaatatttt ttgtaattag tccatcaatt gctttttctc tagcactaaa ttcttgtggc
    15481 atactaaaat ggcaatttcc accattgtta ttcagtgtaa gcggcgcaaa aatcagagga
    15541 acagaactag aagcagcaac agcacgggaa atttctaaat cattcaaatt taaacaaagc
    15601 ccatcaaagg tttcttgcgt aaaggtcagt ttttgaccaa tgttcatgtc tgttgcgcta
    15661 atcacagcaa aaggtccttt acgatttttc actaaatcac caaacttttt accatgataa
    15721 agggcaaaat ttaagcgttc ttgcaataaa tcaccacgcc catattgagg agaacttaaa
    15781 cgaggaagat tggataacga aaacacctct gtaataatat ctttttgaaa gttcgctttt
    15841 aaaaaacggt cagcaaattt aggaatgata tcctttcctt ccaacgcaaa ataagtggct
    15901 aatatcgatc cacctgatac gccaaaggca atatctatat tctctaataa agtattacct
    15961 ttatttgttt ctttcaccct taccttttta aactcttcca acactccata accaagcgcc
    16021 gccgctcttg ttccaccacc tgaaaacatg atgacaacaa tattaccgtc ttttgcactt
    16081 tcagcgatca catttttttg tcgataacct ctttcaacat ttacctgttt aattgtttca
    16141 actggcttat aaacaaccaa actgcaagca gataacacta gcgtaaaggc gaagatgaaa
    16201 agctgtttta ttttgtttag aaaaag
//
LOCUS       DANLXF010000037        16101 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.037, whole genome shotgun sequence.
ACCESSION   DANLXF010000037 DANLXF010000000
VERSION     DANLXF010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16101
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.037"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..277
                     /locus_tag="PXD66_000721"
     CDS             <1..277
                     /locus_tag="PXD66_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319047.1"
                     /translation="NLDEWYSASDKERREFIAKHFAEETVRSDRNTPYDYAMCTGRMF
                     AKQLTRDEVKDYPLANTKPQYLTRDRRIEIMSNLPREILQNCAKYIQ"
     gene            complement(313..1065)
                     /locus_tag="PXD66_000722"
     CDS             complement(313..1065)
                     /locus_tag="PXD66_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDL6319048.1"
                     /translation="MAYKPPFTVTTKMLNLVVEISHKLGTLQLDFERNLHLRKNNRLR
                     SIQASLAIEANSLTIEQVTAIIKGKKVLGHPKDIREVKNAYDAYDEMMHYNPYSIKDF
                     LKAHKFLTADLVNDAGAFRSKDVGIYNFSGQLVHMGARPQFVENLINELFSWAKQDDT
                     PALIKSSVMHYEIEMIHPFSDGNGRMGRLWQNVLLAQWNPLFAWLPIETMVYENQQRY
                     YQMLSLADSQNDSTGFIEFMLEMILETLLKLK"
     gene            complement(1104..2351)
                     /gene="clpX"
                     /locus_tag="PXD66_000723"
     CDS             complement(1104..2351)
                     /gene="clpX"
                     /locus_tag="PXD66_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HDL6319049.1"
                     /translation="MAFEKEPHCSFCGKKRTEVDQLVEGTEGYICNECIEESYALLHG
                     EEEPLIDENQDDEGSFFTNVPTPHELHAHLDDYVIGQEHAKKVLSVAVYNHYKRLRSA
                     LSNHQATNGVELGKSNILLIGPTGSGKTLLAETLARRLNVPFAVADATTLTQAGYVGE
                     DVENVIQKLLMKCDFEAEQAERGIVFIDEIDKITRKSESASITRDVSGEGVQQALLKL
                     LEGTVANINPQGGRKHPKGETIPVDTSKILFICGGAFAGLDKIVEARTNTQGGIGFAA
                     ALKKDKDRQDLTELFKQVEPEDLVKFGLIPELIGRLPVVTPLQELDEEALIQILTEPK
                     NAIIKQYQALFEMEGVELHFTKDALISIAQKAISRKTGARGLRSIVENLLLDTMYDLP
                     TLNAKKVTIGKGCVENGDKPKVE"
     gene            complement(2351..2944)
                     /gene="clpP"
                     /locus_tag="PXD66_000724"
     CDS             complement(2351..2944)
                     /gene="clpP"
                     /locus_tag="PXD66_000724"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDL6319050.1"
                     /translation="MALVPMVVEQTSKGERSYDIYSRLLKERIIFLNGQVEDNMANLI
                     VAQLLFLEAEDPEKDIYLYINSPGGVVTAGLAIYDTMNFIKPDVATLCTGQAASMGAF
                     LLSAGAKGKRFALPHARIMIHQPLGGARGQATDIQIQAEEILRLKATLTRRMAEHSGQ
                     SYEKVLADTERDNFMSAEEAAEYGLIDKVLTSRSEVA"
     gene            3056..3439
                     /locus_tag="PXD66_000725"
     CDS             3056..3439
                     /locus_tag="PXD66_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HDL6319051.1"
                     /translation="MTLTEIYQKFERCKSWEERYRLLIQLSRLLPKPSEQELAQIAEI
                     EGCESRLWFEFQAEPRKVVAYSDARLMQGILFIVSVALTEKTAEELKGFDLKTMFDEL
                     KISQNLTSTRLNGLGQLQKHIMMNL"
     gene            complement(3543..3619)
                     /locus_tag="PXD66_000726"
     tRNA            complement(3543..3619)
                     /locus_tag="PXD66_000726"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3583..3585),aa:Asp,seq:gtc)
     gene            complement(3708..4463)
                     /locus_tag="PXD66_000727"
     CDS             complement(3708..4463)
                     /locus_tag="PXD66_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL6319052.1"
                     /translation="MKKSIYDTPIFFERYQQLRQNPISMNEVVEKPTMFSLLPDLTGK
                     KVLDLGCGTGVHLAHYLTLGAEKVVGLDLSELMLQQAESDLSAHWQKGNDFSLYCLPM
                     ERLNEISEGDFDVVTSSFAFHYIADFSELLAKISQKMTACGTLIFSQEHPIVTCYREG
                     YRWEKNQEKQQVAYRLNYYREEGERDRSWFQQPFKTYHRTMATIVNNLIQGGFEIVQM
                     EEPMLAGQPRWHNEFKDLQHRPPLLFIKAIKQK"
     gene            complement(4523..5518)
                     /gene="trpD"
                     /locus_tag="PXD66_000728"
     CDS             complement(4523..5518)
                     /gene="trpD"
                     /locus_tag="PXD66_000728"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HDL6319053.1"
                     /translation="MQALLDKLFANQALAQAESHRFFSEVIKGNVSNEQLAAALIALK
                     LRGETVDEIAGAAKAALENANPFSTPDYPFADIVGTGGDGHNTINISTTSAIVAATMG
                     YKIAKHGNRSVSSKTGSSDVLSHLGINVDVSPETARLALDETNICFLFAPTYHSGFKY
                     AAPVRQALKTRTIFNILGPLVNPAHAKRQLLGVYSPELVKVYAETVKALGYEHTIVVY
                     GAGLDEVAVHGETQVAEIQNGQIDYYTLTPEDFGIERHSLEDLKGGEPAENAEKVTAI
                     LQGKGKLAHIDAVAANVALLMKTFGEDDLKANVQKVKETLESGKALETLRKLSTY"
     gene            complement(5536..5922)
                     /locus_tag="PXD66_000729"
     CDS             complement(5536..5922)
                     /locus_tag="PXD66_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005543439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HDL6319054.1"
                     /translation="MISVYGLKQTLADRRALIADVIFDCMQMSLGVPKQRHALRFDLL
                     DAENFYPPINRSQDFIVIEINLMQGRSEQLKKRLIKGLFSALHTKVGIVPMDVEITIK
                     EQPEHCWGFRGMTGNEATDLEYQAHR"
     gene            complement(5915..6505)
                     /locus_tag="PXD66_000730"
     CDS             complement(5915..6505)
                     /locus_tag="PXD66_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HDL6319055.1"
                     /translation="MANILFIDNFDSFTYNLVDQFRELGHQVTIFRNDYPLEDFLAKA
                     ETTENCLVALSPGPGNPAEAGNLLEIIRRLKDKVPMIGICLGHQALIEAFGGKVVHTG
                     TVLHGKVSRIEHDNQAMFSGINNPMLVARYHSLMGDELPAEFIVNAKFEHIVMAIRHK
                     TLPICGFQFHPESILTVQGTKLLKQSVEWLLGEKND"
     gene            complement(6515..8071)
                     /gene="trpE"
                     /locus_tag="PXD66_000731"
     CDS             complement(6515..8071)
                     /gene="trpE"
                     /locus_tag="PXD66_000731"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HDL6319056.1"
                     /translation="MPQLTITQTELPYFADTTRVYYALCGERPHTLLLDSAEIQSKNS
                     VKSLLFAKSAVHIFCEDQTVTFHALTPNGEAVLPLIANALSLLNPAPTCKFLDKNDRL
                     QAIFPTISNQLDEDSKLKTTTVFDGLRTVSQLFADSTSPIYLGGLFSYDLVANFIPME
                     NISLQDDGLSCPDYTFYLAEQLVTIDHQTETAVLNTFCFEPNEQTNLNQQAVEFTQKL
                     ANLSHIELPLANQTATSEATANIEDEDFKAIIRKLKHHINIGDVFQIVPSRRFSLPCP
                     NTLASYKQLKINNPSPYMFYMQGENFTLFGASPESALKYSQANRQLEIYPIAGSRPRG
                     FDEQGNIDPELDSRLELELRLDQKELAEHLMLVDLARNDVARVSESGTRRVVDLMQID
                     RYSQIMHLVSRVIGTLRKDLDALHAYQACMNMGTLTGAPKIKAMQLIYQVEQQKRHSY
                     GGAVGYLTSDGNLDTCIVIRSAFVQNGIAYVQAGCGEVLDSDPQLEADETRHKARAVL
                     KAIGQVNAKA"
     gene            8213..8830
                     /locus_tag="PXD66_000732"
     CDS             8213..8830
                     /locus_tag="PXD66_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDL6319057.1"
                     /translation="MTQFFYIHPDNPQQRLINQAVEIIRNGGVIVYPTDSGYALGCAM
                     GDKHAMDRIVAIRKLPENHNFTLVCSDLSELSIYALVNNQAYRLIKNNTPNPYTFILP
                     ATKELPRRLMTKRKTVGIRVPDNAIALALISALGEPILSCSLMLPETEMTESDPDEIR
                     DHLQNRVDLIIHGGYLGQQPTTVVDLTDDVPVIIREGSGDPTSFN"
     gene            8976..10043
                     /gene="rluB"
                     /locus_tag="PXD66_000733"
     CDS             8976..10043
                     /gene="rluB"
                     /locus_tag="PXD66_000733"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HDL6319058.1"
                     /translation="MTTFQKKSTATFRADKRGESRPSFKKEEQKSERKFGKTSEKPPR
                     PTSKPLTQRIAKKVEKTTASEVASQEKKAKVVGEKLQKILARAGQGSRRELEEIIAAG
                     RVSVDGKMATLGDRVQVSSSTKIRIDGHLVNLMPAQKEICRVLMYYKPEGELCTRSDP
                     EGRSTVFDRLPRLSGARWIAVGRLDINTSGLLLFTTDGELANRLMHPSREVEREYSVR
                     VFGNIDDAMLQRLRKGVQLEDGPANFKQIKVVGGTGLNQWFDVTLTEGRNREVRRLWE
                     SQGVEVSRLIRIRYGNIKLDKGLPRGGWEEMGLEQVNYLRELVGLPPETETKVEVGVN
                     RRRTNIRQIRKAVKQHQKYRG"
     gene            10109..11881
                     /locus_tag="PXD66_000734"
     CDS             10109..11881
                     /locus_tag="PXD66_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HDL6319059.1"
                     /translation="MSWDIVATLAVTGIAVFLFATEKMRMDVVAILVLCSLVLLGQVD
                     TKSALSGFSNSATVTVTAMFVLAAGLQNSGALDSVGSLLAKVKSPWLFLLVLCGVNAA
                     VSPFVNNTAVVAVLIPIVIAAAQNIKMAPSKALIPLSFSSQMAGVCTLIGTSTNLLVN
                     AIAQKQGHSGFGMFEFAPLGIIFLVVGVAYLLVTSRFLLPESHLQLEDGEGFGKYVSE
                     LKVNKDSVLIGKSTFESGLNEEFNLFTIGVLRDGERLSTPSHQVLQKHDILLLRGESE
                     NLAKVREKYGLHHVVYGRRENDEDNLDEDLMVAEVMISPISRWIGGTIPILQQRWNKN
                     ATVLGIQRQSKVIRERLRTTAFKMGDILLLTLPKEDMETLRQDKDFIVLSSDLVKTEE
                     SWKSKFALGVMVLVVATAALGWVPISVSALIGAVAMCVAGCLTAEEAYQSIDWKIILV
                     LAGLLPLGEAMANSGAAQFIVDNTLGKVGEFGPLVVLAVLYLLTMVLTEFMSNAGTAV
                     LLTPIAVSTAKMLGVDASPFIIAVMFAAATSFMTPVGYQTNTMVYGAGGYKFTDFIKI
                     GLPLNVIYWILGIVLIPIFFPFNP"
     gene            complement(11971..>16101)
                     /locus_tag="PXD66_000735"
     CDS             complement(11971..>16101)
                     /locus_tag="PXD66_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HDL6319060.1"
                     /translation="VWNHAKRTWIVASELSRSANKSNATNTSLTTNIIKLSTLSLALS
                     AGFASAATYSPTYGTIPDNNPQTSIAIGDGSKVNSTLVSTITGLEGNSGKVSTALGYN
                     TTATADFSTALGGFANASFASTAVGGQATADKRSVAVGYNATAMGLREVFIGDHAGLN
                     HSNSTEYNIGIGYSASSNVTGNNTISIGNTAGDGTSGSHNIAIGTYANAKLAGPTTNV
                     TSDDNIAIGNSALANGVNNYKTTAATGETVIGKATAVGSHANATGIVSSAYGAEANAT
                     AIHSTAIGAKSQANGDNSTAIGYEAKTSGHGATSLGYYANATANLTTAVGTNAGATSD
                     YASAFGREANASGGSATALGNRATASGAASVALGVSAKATNQRTIAIGESSNASAFNA
                     TAIGRNATAEHTDSIALGSNSVTAIAIPTTNATVNGITYSDFAGTNPIATVSIGAEGK
                     ERTITNVAAGRISLSSTDTINGSQLYLTQQAIGNVAATTANILGGGAAVTENGNITFP
                     TYALVNGTPDADKEGKQGRYTTVSAALSALNTAVISPLTFAGDTGTNFERHLGSTVKI
                     KGGSTGILTENNIGVVADGNSTLTIKLAEKVNLGANGSLTTGDTVVNNTGITIANGVA
                     DKPVSLTKSGLDNGGNKIANVAAGDVDTDAVNVSQLKQAISKFATHYVSISDDGIQRA
                     NYDNSGSSGVNPMAIGVATSANGELATALGSEAEANGERTTAVGPRATADGMNATSIG
                     YNANANATNALAVGSAANANADTSTAIGTASTATATRATALGSKSEATGENSTAVGYE
                     ASSIGADSLAAGYNANASGTQSTALGNSANAGGIWSTSVGRNANAAGSSAIALGNSAN
                     AAGVASIALGVSSQATTTAAVALGQNAKATHQGSVALGTNSETVATVATKSATLNGNT
                     YTFAGTTPSSTVSIGSVGNERTLTNVAAGRILDSSTDAINGSQLYAAYTEIDGLNTKV
                     NELSNGALTFVDDAGTEIVRKLGTSLNVKGGADATILTDNNIGVVATDANTLTVKLAK
                     DIDLTPAGSVAVGNSKLNNNGLTINNGPSVTMTGVDAGKLKITNVADGDISPISADAV
                     NGSQLYDTANTIATALGGNSSVNANGAVSAPSYTVVDGAPTNEVSKTVNNVGSAITAL
                     NDAVTSPLTFAGDTGTPSQRKLGSTVTVKGGVSNESQLTDNNIGVISNGNGSLTVKLA
                     KDIKVNSVTAQTVTANIAVADTVKTGDTTIDTNGLTIVGGPSITKTGINAAGTKVTNV
                     KAGTEDTDAVNFSQLKATEKNINNKINNIDSKVNKVDKRLRAGIAGATATAGLPQAYL
                     PGKSMLATAGDTYRNEAAIAVGYSRISDNGKVIYKLTGNSNTRGDFGGSIGMGYQW"
BASE COUNT         4426 a         3285 c         3455 g         4935 t
ORIGIN      
        1 taatttagat gagtggtact cagcttcaga taaagagcga agagaattta tcgcaaaaca
       61 ttttgcagaa gaaacagtaa gaagtgatag aaatacaccg tatgattatg ctatgtgcac
      121 tggtagaatg tttgctaaac aactgactcg agatgaagtc aaagactacc ctttggcaaa
      181 cactaagccg caatatttaa cccgtgatag acggattgag attatgtcta atttacctcg
      241 tgaaatatta caaaattgtg ctaaatacat acagtaattt tatgagtaac accactttgg
      301 aattaagtgg tgttatttta attttaataa tgtctctaaa atcatctcca acataaactc
      361 aataaagccg gtagaatcat tttggctgtc tgcgagagaa agcatttgat aataacgttg
      421 ttgattttcg taaaccattg tttcaatcgg caaccaagcg aaaagtgggt tccattgtgc
      481 taaaaggaca ttttgccata atcgacccat tctgccgttt ccatcactaa aggggtgaat
      541 catttctatt tcatagtgca taaccgagct tttaatgagt gcaggtgtat catcttgttt
      601 agcccaagag aacaactcat taatcaaatt ctctacgaat tgaggacgag cccccatatg
      661 cacaagttgc ccactgaaat tataaatgcc gacatctttt gaacgaaaag cccctgcgtc
      721 atttactaag tcagcagtaa gaaacttatg tgctttgaga aaatctttaa tcgagtaagg
      781 attatagtgc atcatttcat cgtaagcgtc ataggcattt ttgacttcac gaatatcttt
      841 ggggtgtccg agaacctttt tgcccttaat gattgcggta acttgctcaa tcgttaatga
      901 atttgcctca atagctaaag aggcttgaat tgatctcaag cggttatttt ttctaagatg
      961 taaattacgc tcaaaatcca attgtagcgt tcccaattta tgagaaattt ctacaaccag
     1021 attcagcatt ttagtggtaa ctgtaaaagg tggtttataa gccattttgc aataatccta
     1081 aaaaattaaa ccgcttgtca ttgttactcc accttcggct tatcgccgtt ttcaacacag
     1141 cctttgccga tggtgacttt tttcgcattg agggttggga gatcgtacat tgtgtcaagt
     1201 aataagttct cgacaataga acgtaaccca cgagccccgg ttttgcggct aatcgctttt
     1261 tgggcaattg aaattaatgc atctttggtg aagtgcaatt caacgccttc catttcgaat
     1321 aaggcttgat attgcttgat gatcgcattt ttcggctcgg ttaagatttg aatcagagct
     1381 tcttcgtcca attcttgcag cggagtcacc accggtaaac gcccgattaa ttcaggaatt
     1441 aagccgaatt tgactaaatc ttccggttca acttgtttga atagctcggt taaatcttgg
     1501 cgatctttat cttttttcaa tgccgctgca aaaccgatac caccttgagt gttggtacgg
     1561 gcttccacga ttttatctaa accggcaaaa gcaccgccgc aaataaagag aattttggag
     1621 gtgtccaccg gaatggtttc gcctttcggg tgtttgcgac cgccttgtgg gttaatgttc
     1681 gccaccgtgc cttcaagtag ttttaataag gcttgttgaa caccttcgcc cgatacatca
     1741 cgggtaatgg atgcactttc cgatttacgg gtgattttgt cgatttcatc aataaaaaca
     1801 atgcctcgtt ccgcttgttc ggcttcaaaa tcgcatttca ttaataattt ttgaatcacg
     1861 ttttcgacat cttctcccac atagcctgct tgggtaaggg tagtagcatc agctaccgca
     1921 aaaggtacgt ttaaacggcg ggcaagggtt tctgccaaca aggttttacc gctaccggtc
     1981 ggcccgatta ataaaatatt acttttacct aactctacgc cattagtggc ttgatggttt
     2041 gacaatgcac ttcttaagcg tttgtagtgg ttataaacgg ccaccgaaag cacttttttc
     2101 gcgtgttctt gaccgatgac ataatcatct aaatgagcgt gtaattcgtg aggtgtcggc
     2161 acattggtga aaaaactgcc ttcatcgtct tggttttcat caattaacgg ctcttcttca
     2221 ccgtgtaata aggcgtagga ttcctcgata cattcattac aaatatagcc ttccgtgcct
     2281 tcgactaatt gatcgacttc ggtacgtttt ttaccgcaaa aactacagtg gggttctttt
     2341 tcaaatgcca ttacgccacc tcactgcgag aagttaatac tttgtcgatt aagccatact
     2401 cagcggcttc ttccgcagac ataaaattat cacgctcggt atcggctaac actttttcat
     2461 agctctgacc gctgtgttct gccatacggc gggtaagggt agctttcaag cgtaaaattt
     2521 cttccgcttg aatttgaata tcggttgctt gacctctcgc tccacctaaa ggttggtgaa
     2581 tcataatacg agcgtgcggc agagcaaagc gtttaccttt tgccccggct gaaagcaaaa
     2641 atgcccccat tgaagccgct tgccctgtgc aaagggttgc tacatcgggt ttgatgaagt
     2701 tcattgtatc gtaaattgcc aagcctgctg ttaccacgcc acccggtgag ttaatgtata
     2761 aatagatgtc tttttccgga tcttcggctt ctaaaaacag aagttgtgcc acaatcaggt
     2821 ttgccatatt gtcttccacc tgaccgttta aaaaaataat acgttctttt aataaacgtg
     2881 aataaatgtc gtaagaacgt tcgccttttg aggtctgttc caccaccatt gggactaatg
     2941 ccattttgtt ttcctctgtc gtaatacaaa atctcgctat tctagcaaat tttcagaaaa
     3001 tcattaacaa caagcggtca gattgttaaa atcttttgca aatgatgagg aaattatgac
     3061 attaacagaa atttaccaaa aatttgaacg ttgtaaatcg tgggaagaaa gataccgctt
     3121 gttaattcag ctaagtcgct tgttaccaaa accaagtgag caagagttgg cacaaatagc
     3181 ggaaattgag ggctgtgaaa gccgtttatg gtttgaattt caagctgagc cacgaaaagt
     3241 agtggcatat agcgatgccc gtttaatgca ggggatttta tttattgtga gtgtagcttt
     3301 aactgaaaaa acggcagaag agctgaaagg ctttgactta aaaacgatgt ttgatgagct
     3361 aaaaatttca caaaatttaa ccagcacaag gttaaatggg ttgggtcagt tacagaaaca
     3421 tataatgatg aatttgtaat tcaccctcgc cccttgtggg agagggtgaa taaaatccgc
     3481 aggattttat tcgggagagg ggaatataaa aaacctctga tttctcagag gcttaaattt
     3541 gttggcggaa cggacgggac tcgaacccgc gaccccctgc gtgacaggca ggtattctaa
     3601 ccagctgaac taccgctcca tactggtgct aaaataatag tggtttcctc aacattgagc
     3661 aaatagtttt tgcaaaatag tgtttaattg gttgtaaaat aaccacttta tttctgcttg
     3721 atcgccttga taaacagcaa cggcggacgg tgctgtaaat ctttaaattc attgtgccac
     3781 cgaggttggc ctgccagcat tggttcttcc atttgcacaa tctcaaaccc gccttgaata
     3841 aggttattca caatggttgc cattgtgcgg tggtaggttt taaagggttg ttgaaaccaa
     3901 cttctgtccc gttcgccctc ttcccgataa tagttcaagc ggtaagcaac ttgttgtttt
     3961 tcttggtttt tctcccaacg atagccttct cggtaacagg tgacaatcgg gtgttcttgc
     4021 gagaaaatca gcgtgccaca agcagtcatt ttttgcgaaa tttttgcaag tagttcggaa
     4081 aaatcagcaa tataatgaaa cgcaaaagag ctggttacta catcgaaatc gccctcgctg
     4141 atctcattta atcgctccat cggcaggcag taaagggaaa aatcgttgcc tttttgccaa
     4201 tgagcagaaa gatcgctttc tgcctgttgt agcattaatt cagataaatc caaccccacc
     4261 actttttctg caccgagcgt taaataatgg gctaaatgaa ctcctgtgcc acaacctaaa
     4321 tccagcactt ttttgccggt taagtctggc agtagagaga acattgttgg cttttccacc
     4381 acttcattca tacttattgg attttgtcga agttgttggt aacgctcaaa gaaaatcgga
     4441 gtgtcgtaaa tgcttttttt cacttaatag cctttaaaca aatataaaat aaggcgggtt
     4501 aaaacccgcc actacgatat gattagtaag tcgataattt tcgcaaagtt tctaacgctt
     4561 ttccactctc aagcgtttct tttacttttt gcacattggc ttttaaatca tcttctccaa
     4621 aggttttcat taataatgcg acatttgctg ccaccgcatc aatatgagcc agtttacctt
     4681 ttccttgtaa aatagcggtt actttttcgg cattttctgc aggttcaccg cctttgagat
     4741 cttctaacga atggcgttca atgccgaaat cttccggcgt taaggtgtaa tagtcgatct
     4801 gtccgttttg aatttccgca acctgcgttt cgccgtgaac ggcaacttca tctaaacctg
     4861 caccgtatac cacaatagtg tgttcgtaac ctaaggcttt tacggtttca gcatagactt
     4921 tcactaactc aggggagtac acaccgagta attggcgttt agcgtgagcc gggtttacca
     4981 acggccctaa aatattgaaa atcgtgcggg tttttaaggc ttggcgaaca ggggcggcat
     5041 atttgaatcc gctgtggtag gttggggcaa acaaaaagca gatgttggtt tcatctaaag
     5101 caaggcgtgc cgtttcaggt gaaacgtcaa cattaatacc taaatggctt aatacatcgc
     5161 ttgagccggt tttacttgat acgctacggt tgccgtgttt ggcaatttta tagcccattg
     5221 tggcggcaac aatcgcactt gtggtagaaa tgttgatggt attatgccca tcgccacctg
     5281 tgccgacaat atccgcaaaa gggtaatcag gcgttgagaa cggattggca ttttcaagtg
     5341 cggcttttgc agcacctgca atttcatcaa cggtttcgcc acgcagtttt agagcgatta
     5401 aggcagcagc aagttgttca tttgatacat tgcctttaat aacttcacta aaaaaacggt
     5461 gagattccgc ttgggctaag gcttggttgg cgaataattt atctaaaagt gcttgcatag
     5521 tctcttcctt tttaattagc gatgtgcctg atattccaaa tcggttgcct cgttgccggt
     5581 cattccacga aagccccagc aatgctctgg ctgttcttta atggtgattt caacgtccat
     5641 tggcacaatg ccgactttgg tgtgtaaggc actaaatagt cctttaatta agcgtttttt
     5701 gagctgttcg ctccgccctt gcatcaggtt tatttcaatc acaataaaat cttggctacg
     5761 gttaattggg ggatagaaat tttccgcatc caataagtca aaacgcaaag cgtggcgttg
     5821 ttttggtacg ccaaggctca tttgcataca gtcaaaaatc acgtctgcaa tcagggctct
     5881 gcggtctgct aaggtttgtt ttaatccata tacactaatc atttttttct cctaataacc
     5941 attccaccga ctgcttaagt aattttgtgc cttgcacggt taaaatggat tccgggtgga
     6001 actggaaacc gcaaatcggt aaggttttgt ggcgaattgc catcacaata tgttcaaatt
     6061 tcgcattaac gataaattct gccggtaatt catcgcccat taatgagtga taacgagcga
     6121 caagcattgg attgttaatg cctgaaaaca tcgcttggtt atcgtgttca atacgagaaa
     6181 ctttgccgtg caatactgtg ccggtatgca ctactttgcc gccaaaggcc tcaattagag
     6241 cttgatgtcc taaacaaatg ccaatcatcg gtactttgtc tttcagacgg cgaataattt
     6301 ccaataaatt gcctgcctca gccggattac ccgggccggg ggaaagggca accaaacaat
     6361 tttcggtggt ttcagctttg gctaaaaaat cctcaagcgg ataatcattg cggaaaatcg
     6421 ttacctgatg ccctaattca cgaaattgat cgaccaagtt ataggtaaaa gagtcgaaat
     6481 tatcaataaa aagaatattt gccatagttt gtccttatgc ttttgcattc acctgcccaa
     6541 tcgcttttaa caccgcgcgt gctttatggc gtgtttcatc tgcctctaat tgtggatcgg
     6601 aatccagcac ctcaccacaa ccagcttgca cataggcaat gccattttga acaaaggcgg
     6661 aacggatcac gatacaggta tctaaattgc cgtctgaggt taaatagccc accgcaccgc
     6721 cgtagctatg gcgtttttgt tgctcgactt ggtagatcag ttgcattgcc ttaattttcg
     6781 gagcaccagt tagcgttccc atattcatac aagcttgata agcgtgcaga gcgtcaagat
     6841 cttttctaag tgtgccgatc acacgagaaa ctaagtgcat aatttgcgaa tagcggtcaa
     6901 tttgcattaa atcaaccaca cggcgagtgc cggattcgct cacacgagcc acatcattgc
     6961 gggcaagatc gactaacatt aaatgttcgg caagctcttt ttggtctaaa cgcagttcta
     7021 attccaaacg ggaatctaat tcaggatcga tattaccttg ttcatcaaaa ccgcgtgggc
     7081 gagagccggc aatcgggtaa atttccaact ggcggttggc ttgcgaatat ttcaacgcac
     7141 tttcaggcga cgccccaaac agagtgaaat tttcgccctg catatagaac atatacgggc
     7201 ttggattgtt gattttgagc tgtttatagc tggcaagagt attcgggcaa ggcaagctga
     7261 aacggcgaga gggcacaatt tggaacacat caccgatatt aatatggtgt ttgagtttgc
     7321 ggataatcgc tttgaaatct tcatcttcaa tattggcggt tgcctcactc gttgcggttt
     7381 ggttcgctag aggcagctca atatgtgaca gatttgcaag tttttgcgta aattcgaccg
     7441 cttgttggtt caaattggtt tgttcattcg gttcaaaaca gaaggtgttt agcaccgcag
     7501 tttcggtttg gtggtcgatt gtaaccagtt gctctgctaa atagaaagtg taatctgggc
     7561 aacttaagcc atcatcttgc aggctgatat tttccatcgg aataaaattt gccaccaaat
     7621 cgtagctaaa taatccgccc aaatagatag gcgatgtgga atcagcaaac agttggctca
     7681 ccgttcttag cccgtcaaac acggtggtgg ttttgagctt gctgtcttca tctaactggt
     7741 tgctgatggt tgggaaaatc gcttgcaagc ggtcgttttt gtctaaaaat ttgcaagtcg
     7801 gagcagggtt taacaggctc aatgcgtttg caatcaaagg taaaaccgct tctccgtttg
     7861 gggttaaagc gtggaaagtc acggtttgat cttcacagaa aatatgcact gccgatttcg
     7921 caaacagcaa actttttaca ctgtttttac tttgaatttc cgctgaatct aataatagcg
     7981 tatgtgggcg ttcgccgcat aatgcgtaat aaacacgggt cgtatcggca aaatagggta
     8041 attctgtttg agtaatcgtt aattgaggca tagcaaattc ctattcaatt tttcgtacta
     8101 ttaaactagt acattaaaaa gaagtcaagc aattattcca gataattatg cgagcttggg
     8161 cgaacttggt gtataattag cccaattttt agaaagtcga aggcttgaga ctatgacaca
     8221 atttttttat atccaccccg acaacccaca gcaacgctta attaaccaag cggtagagat
     8281 tattcgaaac ggtggcgtaa ttgtttaccc taccgattca ggctatgctc tcggttgtgc
     8341 aatgggcgat aagcacgcaa tggatagaat tgtagcaatt cgtaaattgc cggaaaatca
     8401 caattttacc ttagtttgta gtgatttatc cgagctttct atctacgcgt tggtcaataa
     8461 tcaagcctat cgcttaatta agaataatac cccgaatcct tatactttta ttctgccggc
     8521 aactaaggaa cttcctcgca ggctgatgac aaagcgtaaa acagtgggga ttcgtgtgcc
     8581 ggataatgcg atcgcattgg cgttaatttc cgccttaggt gagccgattc tttcttgctc
     8641 gttaatgttg ccggagacgg aaatgaccga gtcagaccct gatgaaattc gggatcattt
     8701 gcaaaatcgg gtggatttaa ttatccacgg tggttattta gggcagcagc ctacaacggt
     8761 agttgactta accgatgacg tgccggttat tattcgagaa ggatcgggcg atccaacatc
     8821 gtttaattaa attgatgttg tatgattaat atatagaatt atgcgtaggg gggctgtgcc
     8881 agtccgtatt aatatcgtta tttggacagg cacaggacct gtccctacaa tgaaaatcaa
     8941 attcagacgc ttgtgaaagc gacaaaagga accttatgac aacttttcaa aaaaaatcaa
     9001 ctgcgacttt ccgtgcagac aaacgtggtg aaagccgtcc ctcatttaaa aaagaagagc
     9061 aaaaatctga acgcaaattt ggtaaaacgt cagaaaaacc accacgtcca acttctaagc
     9121 cattaacgca aagaattgca aaaaaagtgg aaaaaacgac cgctagtgaa gtggcatctc
     9181 aagagaaaaa agccaaagta gtaggtgaaa agctacaaaa aatcttagcg cgagcagggc
     9241 aaggttctcg ccgtgaatta gaagagatta tcgctgctgg acgagtgagc gtggacggta
     9301 aaatggctac tcttggtgat cgtgtgcagg ttagctcatc gactaaaatc cgcattgacg
     9361 gacatttagt taatttaatg ccggcacaaa aagagatctg ccgtgtatta atgtattaca
     9421 aaccggaagg ggaactttgc acccgttccg atccggaagg acgatccacc gtatttgacc
     9481 gcttgcctcg tttaagtggc gctcgttgga ttgcggtcgg gcggttagat attaacacct
     9541 caggcttatt attgttcact accgatggcg aattagccaa ccgtttaatg cacccaagcc
     9601 gtgaagtcga acgtgaatat tcggtgcgtg tgttcggcaa cattgacgat gcgatgttac
     9661 aacgcttacg caaaggcgtt cagttggaag acggaccggc aaactttaaa caaattaagg
     9721 ttgtgggtgg cacaggcttg aaccaatggt ttgatgtgac tttaaccgaa gggcgtaacc
     9781 gtgaggttcg ccgcctgtgg gaatcgcaag gggtagaagt cagccgtttg attcgtatcc
     9841 gttacggcaa tatcaaatta gataaaggct tacctcgtgg cggctgggaa gaaatgggct
     9901 tagaacaagt caattacttg cgtgagctgg tagggttacc gccggagact gaaactaaag
     9961 tggaagtggg ggtaaaccgc cgccgaacca atatccgtca aattcgtaaa gcggtaaaac
    10021 aacatcaaaa atatcgtggc taaacaccat ctttggggct gcattagttc tgctaatgca
    10081 gctattttct ttcaaaaaag gagcatttat gtcttgggat attgttgcaa cattggcggt
    10141 cactgggatt gccgtatttt tatttgcaac tgaaaaaatg cgaatggacg tagttgcgat
    10201 tttagtgctg tgttcattag tattattagg gcaggttgat accaaatctg ctctgagtgg
    10261 attttcaaac tcagctactg taaccgtaac tgcaatgttt gtattagctg ctggtttaca
    10321 aaatagtggt gctttagata gtgtaggcag tttacttgct aaggtaaaat ctccttggtt
    10381 attcttgctt gtactctgtg gcgtaaatgc tgctgtttct ccttttgtga ataatacggc
    10441 agtggtcgca gtattaattc cgattgtgat tgccgcagca cagaatataa aaatggctcc
    10501 ttccaaagcc cttattccgt tatcattttc ttctcaaatg gcaggtgttt gtacactgat
    10561 cggcacttcc actaatctat tagttaatgc aatcgctcaa aagcaagggc attccggttt
    10621 cggtatgttt gaatttgccc ctttggggat tattttttta gtggttgggg ttgcctattt
    10681 attagtgacc agccgtttct tattacccga atcacatttg caacttgaag atggtgaggg
    10741 gtttggaaaa tatgtttctg aacttaaagt aaataaagac tctgtattaa ttggcaaaag
    10801 tacatttgaa tcaggattaa atgaagaatt taatctattt actattggtg ttttgcgtga
    10861 tggggaaaga ttatcgacac cgagtcatca agtattgcaa aaacatgata ttttattgtt
    10921 gcggggtgaa tcggaaaatt tagcaaaagt acgagaaaaa tatggattgc accacgttgt
    10981 gtatggacgc cgtgagaatg atgaagataa tctggatgaa gacttgatgg tggctgaagt
    11041 gatgatttct cctatttctc gttggatagg agggactatt cctattttac aacagcgttg
    11101 gaataaaaat gcaacggtgt taggcattca acgccaaagt aaagtaattc gtgagcgttt
    11161 acgcaccact gcttttaaaa tgggggatat tttattactc acattaccaa aagaggatat
    11221 ggagacgtta cgccaagata aagactttat tgtgttatct tctgatttag tcaaaacgga
    11281 ggaatcgtgg aaaagtaaat ttgctttagg ggtaatggtt ttggtggtgg caacagcagc
    11341 cttaggctgg gttcctattt cagtgagtgc cttaattggt gcagttgcga tgtgtgttgc
    11401 tggttgttta acggctgaag aagcctatca atctattgac tggaaaatca ttttagtctt
    11461 agctggatta ctgccattag gtgaagcgat ggcaaatagt ggggcggcgc aatttattgt
    11521 tgataacacg cttggtaaag taggggagtt cgggccttta gtggtgttag ctgtattata
    11581 tttactgaca atggtgttaa ccgaatttat gagtaatgca gggactgccg tgttgcttac
    11641 gcctattgcg gtttctaccg ctaaaatgtt aggggtagat gcttccccat tcattattgc
    11701 cgtaatgttt gcggctgcga ccagctttat gacccctgtt ggctatcaaa ccaacacgat
    11761 ggtatacggt gcaggtggct ataaattcac agattttatt aaaattggtt taccgctaaa
    11821 tgtaatctat tggattttag ggatagtatt aattccgatt ttcttccctt ttaatccata
    11881 attgatagat aattatagaa aaaaggttta cagatcagaa actctgtaaa ccttttttat
    11941 tcagcaataa atcaattttc tattggaaaa ttaccattga taacccatgc caatactacc
    12001 accaaagtcg cctctggtat tgctgttacc agttaattta tagataacct tcccgttatc
    12061 tgaaatccga gaataaccta ctgcgattgc agcttcatta cggtatgtat ctccagccgt
    12121 tgctaacata cttttacctg gtagatatgc ttgcggtaaa cctgcggttg cagttgcacc
    12181 agcaatacca gcgcgtaaac gtttatcaac tttgtttact tttgaatcaa tattattgat
    12241 cttattattg atattttttt ctgttgcttt taattggcta aagttaactg catcagtatc
    12301 ttcagttcct gcttttacgt tagtcacttt tgttcctgct gcattaatac ctgttttagt
    12361 tatactcggt ccccctacaa tagttaatcc attagtatca atggttgtat caccagtctt
    12421 aacagtatcg gctacagcaa tgttagctgt tactgtttga gctgtgacag aattaacttt
    12481 aatatcttta gctaatttaa ccgttaatga accattacca tttgaaataa ccccgatatt
    12541 attatcagtt agttgtgatt cgttagatac cccacctttt actgtaacag tagaacctaa
    12601 tttacgttga gatggcgtgc cggtatcacc tgcaaatgtt agtgggctgg ttactgcatc
    12661 atttaacgca gtaattgcag agcctacatt attgaccgtt ttactaactt catttgtagg
    12721 agcgccatca accacagtat agcttggggc tgaaacagca ccatttgcat ttacacttga
    12781 attaccacct aaagcggtag caatagtatt tgccgtatcg taaagttggc taccattaac
    12841 tgcatctgct gagataggtg aaatatcacc atcagctaca ttagttattt ttaatttgcc
    12901 agcatcgaca cctgtcattg tgacacttgg gccattatta atggttaagc cgttgttatt
    12961 tagttttgaa ttaccaacag ctacggagcc ggcaggagtt aaatcaatgt ctttcgctaa
    13021 tttaactgtt aatgtgttag catctgttgc cactacaccg atattattat cagtcaatat
    13081 ggttgcatcg gccccacctt taacattcag agaagtacct aatttacgaa caatttcagt
    13141 accagcatca tctacaaaag taagtgctcc attacttaac tcattcactt tcgtgtttaa
    13201 accatcaatt tcggtataag cagcataaag ttgtgaaccg ttgatagcat ccgttgatga
    13261 gtccaaaata cgacctgctg caacattcgt taaagtacgt tcattgccta cagaaccaat
    13321 acttacagtt gagcttggcg tagtacctgc aaatgtgtat gtattgccat ttagcgttgc
    13381 actttttgtt gcaactgtgg ctactgtttc agagttagta cctaatgcaa cagaaccttg
    13441 gtgtgttgct tttgcatttt ggccaagagc cactgcagca gttgttgttg cttgtgatga
    13501 aacacctaat gcaatagaag ctacaccggc tgcatttgca ctattaccta aagcaatcgc
    13561 agagctacct gcagcattgg catttctacc cacagaagtt gaccatattc cacctgcatt
    13621 agcagagtta cctaaagcgg ttgactgtgt acccgaagca tttgcgttat agcctgcagc
    13681 aagagaatct gcaccgattg aacttgcttc ataacctaca gcggttgaat tttcaccggt
    13741 agcttcagat ttagagccta atgcagttgc acgggtagct gtcgctgttg aagctgtacc
    13801 aatagctgtt gaggtatcag catttgcatt ggctgcagaa ccaaccgcga gggcatttgt
    13861 tgcatttgca ttcgcattgt agcctataga agtggcattc ataccatcag ctgttgcgcg
    13921 aggaccaact gcagttgtac gttcaccgtt tgcttctgct tcagagccta atgcggttgc
    13981 cagctcacca ttagcacttg ttgcaacgcc gatagccatt ggattaacac cgctagagcc
    14041 tgaattatca tagttagcac gttgaatgcc atcatctgaa atactaacat aatgagttgc
    14101 aaatttgctg atagcttgtt tgagttgact gacattgacc gcatcagtat ctacatcgcc
    14161 tgcggcaaca ttggcgattt tattaccgcc attatctaag ccagatttag taagacttac
    14221 cggtttgtct gcaacgccgt tagcgatggt gatacctgta ttgttaacaa cggtatcacc
    14281 tgttgttaat gaaccattag cgcctaagtt aactttttct gctaatttta tggttaaagt
    14341 actattgcca tcagctacaa caccaatatt attctcggtt aatatgcctg tagatccgcc
    14401 tttgatcttg actgttgaac ctagatggcg ctcaaaatta gttcctgtat caccggcaaa
    14461 ggttaacgga ctgatcaccg ctgtgtttaa tgcgcttagt gctgcagaaa cagtagtgta
    14521 tctcccttgt ttgccttctt tatctgcatc tggtgtgcca ttcactaaag cgtaggttgg
    14581 gaaagtgata tttccatttt cagtaacagc tgccccacca cctaagatat ttgcggttgt
    14641 tgcagcaaca ttacctatag cttgttgtgt taaatataat tgtgaaccgt ttatggtatc
    14701 tgtagagctt aaagatatac gtcctgcagc aacattggtg atagttctct ctttgccttc
    14761 cgccccaata cttactgtag caattggatt tgttccagca aagtcactat atgtaatacc
    14821 atttacagtt gcatttgtag ttggtatggc tatagctgta actgagttgc tacctagcgc
    14881 tatcgaatcg gtatgttcag cagtggcatt tcgacctata gcggttgcat taaaggcgga
    14941 agcatttgat gattctccga ttgcaattgt gcgttgattt gttgctttag cgcttacccc
    15001 tagtgcaaca gaagccgcgc ctgatgcagt agcacgattg cctaaagcgg ttgcagaacc
    15061 tccacttgca ttggcttcac gaccaaatgc agaagcataa tcgctggttg cacccgcatt
    15121 agtaccaaca gcagtagtta aatttgctgt tgcatttgca taataaccta atgaggttgc
    15181 accgtggcct gaagtcttcg cttcataacc aattgctgtt gagttatcgc catttgcttg
    15241 agattttgca ccaattgctg tggagtgaat agctgttgcg ttagcttcag caccataggc
    15301 ggagctaaca ataccagttg cattggcatg actacctaca gcagttgctt ttcctattac
    15361 cgtttctcct gttgctgctg tagttttgta attattaaca ccatttgcaa gagcggaatt
    15421 accgatggcg atattatcat cactggttac atttgtagta ggtcctgcta gttttgcatt
    15481 agcataagta ccaattgcaa tattgtggct accactagta ccgtctccag cagtattacc
    15541 aatagaaatc gtattgtttc ctgttacatt agagctagcc gaataaccaa taccaatatt
    15601 gtattctgtg ctgttagagt gatttaatcc ggcatgatcg ccaataaaaa cttcacgtaa
    15661 gcccattgcg gttgcattgt accctactgc tacggaacgt ttatcggcag tggcttgccc
    15721 accaacagct gttgaagcaa aactagcgtt agcaaagcct cctaatgcag ttgaaaaatc
    15781 ggctgtcgcg gttgtgttat accctaatgc tgtagaaact ttaccgctat ttccttctaa
    15841 gcctgttatt gtgcttacta gtgttgagtt aactttagat ccatcaccaa ttgcgataga
    15901 tgtttgtggg ttattatctg gtatggtccc ataggttggt gaataggttg cagcacttgc
    15961 aaaacctgca gaaagcgcaa gacttagtgt gctgagttta ataatattag tggttagtga
    16021 tgtatttgtt gcattagatt tattagcact tcttgataat tcagaagcaa caatccaagt
    16081 tcgttttgca tggttccaaa c
//
LOCUS       DANLXF010000038        16045 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.038, whole genome shotgun sequence.
ACCESSION   DANLXF010000038 DANLXF010000000
VERSION     DANLXF010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16045
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.038"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            8..754
                     /gene="znuC"
                     /locus_tag="PXD66_000736"
     CDS             8..754
                     /gene="znuC"
                     /locus_tag="PXD66_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HDL6319061.1"
                     /translation="MVSLENIEVSFNGQKALQNINLSIYPNSITTIVGPNGGGKSTLL
                     KILLKLLSPNKGKVIHQKGLKIGYVPQKLHLEPSMPITVYRFLALKPHTTKEMINQAL
                     DLFSITHLTENSMQKLSGGELQRVLLARAILDRPQLLVLDEPMQGVDIIGQTELYQLL
                     NKTRDLLNCAILMVSHDLNIVMANTDEVLCINKHICCAGSPEVITHDPSFIHFFGDQF
                     ARNVAFYSHKHNHHHNLHGDICLGECSCEH"
     gene            919..3732
                     /gene="sucA"
                     /locus_tag="PXD66_000737"
     CDS             919..3732
                     /gene="sucA"
                     /locus_tag="PXD66_000737"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HDL6319062.1"
                     /translation="MQYTHFDDWLASSALGGANQSYIEEIYEQYLENPANVDESWRNI
                     FDSFPKTPQVEQAHTPIRDYFRKLAREQQPQSVTIIDPEAGAKQVRLLQWINAHRFRG
                     YLEAKLDPINYYRWKTSSVPELDYKYHGFTDEDLNETFTVGRYVYDKDTMKLSELDKA
                     LKETYLGPIGLEFMHVQDIEQRNWLQAKMESVLNKPLFTKAEKLNLLSELTAADGLER
                     YLGAKFPGAKRFSLEGSDAFIPMMKEIIRHASRQGMTDVVLGMAHRGRLNMLVNVFGK
                     RPAELFDEFAGKHADESRTGDVKYHQGFSSNFAVDDKNVHLTLAFNPSHLEIVSPVVI
                     GSVRARQERMRDIEHEKVLAVTVHGDSAVAGQGVVQETLNMANVRGYKVGGTIRIVIN
                     NQIGFTTSNPNDTRSTEFCTDIAKMIQAPIIHVNGDDPEAVAFAARMAVEYRTKFKRD
                     IFIDLISYRRHGHNEADEPLATQPMMYSIIKKHPTPRKVYADRLITEGVLTNEQEIEM
                     LNLYRDALDNGECVVPEWREMDMAAVDWLQYLNYDWTALYESKFPEERFLTIAKRVCE
                     YPASLKAHSRVEKIYTDRKEMYEGKKLFDWGMAETMAYATLLDEGTHVRLSGEDAGRG
                     TFFHRHAVIHNQNDGTGYVPLTQLHANQGRFQVWDSVLSEEAVLAFEYGFATTDPKTL
                     TIWEAQFGDFANGAQIVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARVERY
                     LQLCAQQNIQVCVPSTPAQVYHMLRRQAIRKMRRPLVAITPKSLLRHPLAVSSLDELI
                     NGSFQNVIGELDNIDPKQVKRVVMCSGKVYYDLIEKRRENNQTDVAIIRIEQLYPFPL
                     EEMQQILEPYAHVKDFVWCQEEPLNQGAWYCSKHNFEASLPEDAKLTYAGRPASASPA
                     VGYPSLHAKQQKQLVEDALTIAE"
     gene            3835..5064
                     /gene="odhB"
                     /locus_tag="PXD66_000738"
     CDS             3835..5064
                     /gene="odhB"
                     /locus_tag="PXD66_000738"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HDL6319063.1"
                     /translation="MTIEILTPDLPESVADATVATWHKKVGDNVKRDEILVEIETDKV
                     VLEVPASSDGILAEITQEQGATVVSKQSLGKLVVAKAGDISSATIEQKTESTPSDRKH
                     AAIENSHANADDQGPAIRRLLAEHDLQAADIQGSGVGGRITREDIEKEIAKRVQINQV
                     KGSATSQNTVSTVAFNSRSEKRVPMTRLRKRIAERLLEAKNTTAMLTTFNELDMQPIM
                     NLRKTYGEKFEKQHGVRLGFMSFYIKAVVEALKRYPEINASIDGDDVVYHNYFDISIA
                     VSTPRGLVTPVLRDCDKMSMADIEKKIKELAEKGRDGKLTVEDLTGGNFTITNGGVFG
                     SLMSTPIINPPQSAILGMHAIKDRPVAIDGQVVIRPMMYLALSYDHRLIDGKESVGFL
                     VAVKELLEDPTRLLLEI"
     gene            5357..6517
                     /gene="sucC"
                     /locus_tag="PXD66_000739"
     CDS             5357..6517
                     /gene="sucC"
                     /locus_tag="PXD66_000739"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HDL6319064.1"
                     /translation="MNLHEYQAKQIFKQYGLPVSQGIACKTAEEAVEAIKQLQGEQWA
                     IKCQVHAGGRGKAGGVKLVRNEAEVREFADKWLGKRLVTFQTDANGQPVNTLYLEETC
                     NIDRELYLGAVVDRSAQKVIFMASSAGGMNIEEVAEKTPELIHKAVIDPLTGGQGYQG
                     RELAFKLGLKGDEVKQFADIFVKLANLFVEKDLALLEVNPLVITKEGQLLCLDAKMSI
                     DSNALYRHPELKELQDPSQDDEREAEAEKWNLNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLYGGKPANFLDVGGGATKERVAAAFKLILSDPNVKAVLVNIFGGIVRCDLIADGVIA
                     AVNEVGVNVPVIVRLEGTNAAQGREILANSGLSILAASSLKQAAELAVKSAN"
     gene            6526..7395
                     /gene="sucD"
                     /locus_tag="PXD66_000740"
     CDS             6526..7395
                     /gene="sucD"
                     /locus_tag="PXD66_000740"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HDL6319065.1"
                     /translation="MSILIDKNTKVICQGFTGGQGTFHSEQALAYGTQLVGGVSPNKG
                     GTTHLGLPVFNTVREAVEATGATATVIYVPAPGCKDAILEAIDAGIQLIVCITEGIPT
                     LDMLQVKQRLNQTGVRMIGPNCPGVITPDECKIGIMPGNIHKKGNIGIVSRSGTLTYE
                     AVKQTTDEGFGQSTCVGIGGDPIPGSNFIDILKLFQDDPETEAIVMIGEIGGSAEEEA
                     AAFIKENVTKPVVSYIAGVTAPKGKRMGHAGAIISGGKGTADEKFKILEDAGVTTVRS
                     LAEIGSALRKILG"
     gene            complement(7466..8911)
                     /locus_tag="PXD66_000741"
     CDS             complement(7466..8911)
                     /locus_tag="PXD66_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDL6319066.1"
                     /translation="MDSIILFFESVLTWIVNTIDGPLWDVTIILLLGAGLFFTLTTGF
                     VQLRLLPQSLREMWGGRSTEGKSLTPFQAFTTGLASRVGVGNIGGVATAIALGGPGAV
                     FWMWITALMGMSSAFAESSLAQLYKTKDPNGMFRGGPAYYITRGLKLPSLAIAFAVTL
                     IFTFGFAFNAVQSNSIVEATKNAWDWNPVYVGMTLVVITGLIISGGVKRIGKVSAQVV
                     PMMALFYLIIAVIILGMNIEKVPSIISLIIESAFDFSAAAGGTFGALVSKTMLMGIKR
                     GLFSNEAGMGSAPNSAATSDAKHPASQGLIQMLGVFVDTIVVCTCTAVIILMSNNYGG
                     ETLRGISLTQSALQFHLGEFGLHFLAFVILLFCFTSIIGNYAYAETNIRYIKNSPWFV
                     WGFRAIVLFFVYFGAVRDGGIVWAFADTVMASMAVINLVAILILAPIVWIILKDYLRQ
                     VKSGKEPVFRIEEHQDLIHKGVDPLIWKSKE"
     gene            complement(9016..10002)
                     /locus_tag="PXD66_000742"
     CDS             complement(9016..10002)
                     /locus_tag="PXD66_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt transporter"
                     /protein_id="HDL6319067.1"
                     /translation="MRLKATYWGMIVIGFLSLFALYQLYPMILFKVMEWQKNFNLQLS
                     ASLNELAENQSKAGITLVFISFLYGVFHAVGPGHGKFILSSYLSFEKTKLNQAMKITF
                     ASALVQGLVAISLVTVIVVIFTLSRQYFNLTLKWVERGSFGLMILFGIYWCYQVYRTV
                     NRARKPIIKSIRISQKKAGKLPLVTQKGHIHTENCSCRHKHLPTSNEMQQVRDWKAQL
                     MLVLSIGSRPCSGAILVLFLSYTLNLYAWGVISALAMAVGTGLTLSLFAYLVIIARNK
                     AVKVSQWYFSAQINRNIVWILKFTLGLALILFGMVLFHSSFIDAGTGVFFKR"
     gene            complement(9993..10631)
                     /locus_tag="PXD66_000743"
     CDS             complement(9993..10631)
                     /locus_tag="PXD66_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1007 family protein"
                     /protein_id="HDL6319068.1"
                     /translation="MKFIKTIITLIALYSMPVFSHPHSFVDLKTNVIVEGSMLKSFQM
                     EWMLDEIASSELIYEVKNSQDKEKTQQNITAEMVQTALQNHYFSTLYDSQNQPIKFTT
                     KPTATRFEIKNNRVIFYITFHLAKPHELKQSKVRFYTYEPSYYIAMEYNRPADVNTSN
                     ASCKATLVQPQVDSKLRLYASGLDKNQSLDMPENGDYSLGAQFAQKVEIICD"
     gene            complement(10692..11070)
                     /gene="rnpB"
                     /locus_tag="PXD66_000744"
     ncRNA           complement(10692..11070)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PXD66_000744"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            11815..12405
                     /locus_tag="PXD66_000745"
     CDS             11815..12405
                     /locus_tag="PXD66_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319069.1"
                     /translation="MTTQITTAQINDLLKSHDIQGYANEWQGRRIYINLSAKNNESKG
                     DRNYQLYIDLATGELVSKLGKGLTTRAFDAAVKAFENAFNAKDTAENTAPVESKETEQ
                     ENKQETTKLVITKPTLKGSEKQIDSANEIINLFIAKIGDTSIPVTATQEQITQAQAMV
                     DNFFAMTNAGKWIEKFKGLNDNSEPKQFWLSVKYGW"
     gene            12475..12993
                     /locus_tag="PXD66_000746"
     CDS             12475..12993
                     /locus_tag="PXD66_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319070.1"
                     /translation="MTTQVTPQIMRIIGQIVAATYGDDVPTNVQTIILRYPIRGIGFI
                     SSRRELSINNGEIARLMDKIPGDLEDPKDGMPFDCQGAFWLGYYQYCKLSNDVKNYTS
                     KELSIIGESLYGTQWQSNLARDLRLSDARRVREWVAGERKIPFGVWADLTELVKAKKA
                     NLSSILKKLTID"
     gene            complement(13140..13451)
                     /locus_tag="PXD66_000747"
     CDS             complement(13140..13451)
                     /locus_tag="PXD66_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319071.1"
                     /translation="MTKTKHKHPLAEAFYQLGKDVGEINRRLVVVDEQSLKLQELKQT
                     INEQTEQIKTLGTVVNKLIDALQQSQSLNVRSESAVAATENQDHLNDGVQYQGPGRAR
                     A"
     gene            complement(13444..13593)
                     /locus_tag="PXD66_000748"
     CDS             complement(13444..13593)
                     /locus_tag="PXD66_000748"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319072.1"
                     /translation="MDNILITIHPTETIDITIDKLVVIHIGEEYGDTPDFLTIYQQAK
                     ELNDD"
     gene            complement(13827..14120)
                     /locus_tag="PXD66_000749"
     CDS             complement(13827..14120)
                     /locus_tag="PXD66_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319073.1"
                     /translation="MTTFNKILNPMYSTIASYSTQDDGSLNAKYVVGTGDDTDGEVTN
                     FVIITSEYKYIDAQSAKAITDAPLTKEDIGKTPTQIMLGRIYNHLKETGQIVV"
     gene            complement(14243..>16045)
                     /locus_tag="PXD66_000750"
     CDS             complement(14243..>16045)
                     /locus_tag="PXD66_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HDL6319074.1"
                     /translation="IQETLANADSALSSRIDAVTASVGSNLAKITQVSNAVAGVDGKL
                     SATHTIKTETIAGGRKAIAGIALGAAADNRNAESSVIVMADKFEVVKNAQDGQPVRLF
                     GVAQNKVAINGNLIATGTIKGSHIDADSVRAGILTAGAIRTEHLAAGQISADKLAIGL
                     GGNLLVNPIFATPDLSIAPFGWNYWRGEVGDRLNIDTRNIFNRNEQDNYGLKNGGLPN
                     ELVFSMRYSTTKETSDTGRIGWLSQDINVVPNKKYIASVWLACHRGQAKLVIENIEKP
                     GGAYLGWIGDSEVITGYSANQGEFKNMKRVKVVFTAPTSGCIRFALRLDNIQGKANPF
                     LFARRPMLEEASDNVTDITQPSPWQNAGVTAIHGGSIVTNTVTAQQIAANTITANEIA
                     TGTIAARNMAANSINASHIVGSSITADKLNVNNLAAISANLGKVTAGTITGTTITGNT
                     ISGGSINGTTISGTTISGGTIKGARLEGATGKFTGELEVSQLIGGGVIEQVTGKLKYS
                     HRNRIYEGQEHGENSSSPIYRHEYIYKATVTINAAPTRRYIEIIGSESFILEANQSIT
                     KTIEKSLNRNASGSSLDFIVLAYSISTAKYISIS"
BASE COUNT         4967 a         3414 c         3158 g         4506 t
ORIGIN      
        1 gacatcactg gtttctcttg agaatattga agtgtctttc aatggacaaa aagcattaca
       61 aaatattaat ttatctattt atcctaactc tatcacgacg atagtcgggc caaatggtgg
      121 aggaaaatct accctgctta aaattttgct aaaattactt agcccaaata aaggaaaggt
      181 tattcatcaa aaaggactca aaattggcta tgtaccacaa aaattgcatt tagaaccctc
      241 gatgcccatt actgtttatc gatttcttgc gctaaaacca catactacca aagaaatgat
      301 taaccaagca ctagatctgt tttctattac ccatctcact gaaaatagta tgcaaaaatt
      361 atcgggtggt gaacttcaac gtgtattgct ggctcgggca attttagatc gtccgcaact
      421 cttagtgtta gatgaaccaa tgcaaggcgt tgatattata ggccaaaccg aactctatca
      481 attacttaat aaaacaaggg atctccttaa ctgtgctatt ttaatggtct cacacgactt
      541 aaatattgtg atggcgaata cagatgaagt gctttgcatc aataaacata tttgttgtgc
      601 cggaagcccc gaagtcatta cacacgatcc gagctttatc catttttttg gcgaccaatt
      661 tgcacgtaat gttgcatttt attcacataa acataaccat caccataatt tacatggcga
      721 tatctgcctt ggtgaatgta gttgtgagca ctgataggcg tcctttttac caaatgttta
      781 aaatttgtaa aaataatgct aaaaataacc gcttgcagca caagatttat ctaaaaatca
      841 gacttacttc acattttatt tgcttaaatt ggagtataat aagggcgtga tttcatacca
      901 caaacaggtc aggtctttat gcaatataca cattttgatg attggcttgc atcatctgca
      961 ttaggcggtg caaaccaatc ttatatagaa gaaatttatg aacagtatct agaaaatcca
     1021 gcaaatgttg atgaaagctg gagaaacatc tttgattcat ttcctaaaac tccccaagtt
     1081 gaacaagctc acacccctat tcgtgactat ttccgtaaat tagcccgtga acagcaacct
     1141 caatccgtca ctattattga cccagaagca ggggcgaaac aagttcgctt actgcaatgg
     1201 attaacgctc accgtttccg tggctattta gaggcaaaac tagacccaat taactattac
     1261 cgttggaaaa cctccagcgt gcctgaattg gactacaaat atcacggctt caccgatgaa
     1321 gatctcaacg aaacttttac ggtcggtcgc tatgtctatg ataaagacac aatgaagctc
     1381 agcgaactgg ataaagccct aaaagaaact tatttagggc ctattggttt agaatttatg
     1441 cacgttcaag atattgaaca acgcaactgg cttcaagcca aaatggaaag cgtgctaaac
     1501 aaaccgctgt tcaccaaagc agaaaaactg aatttattaa gcgaattgac cgctgccgat
     1561 ggtttagagc gttacttagg ggcaaaattc ccgggggcaa aacggttctc attggaaggt
     1621 agtgatgcct ttattccgat gatgaaagaa attatccgcc acgcttcccg tcaaggtatg
     1681 accgatgtgg ttttaggtat ggcacaccgt ggtcgtttga atatgttagt gaacgtattt
     1741 ggtaaacgcc cggcagaatt gtttgatgaa tttgccggca aacacgctga tgaaagccgc
     1801 acgggcgatg tgaaatacca ccaaggtttc tcctcaaact ttgccgtaga tgataaaaac
     1861 gttcacctta cccttgcttt caacccatcg catttagaga ttgttagccc ggtggtaatc
     1921 ggctcagtgc gtgcaagaca agaacgtatg cgtgatatcg aacacgaaaa ggtgcttgcg
     1981 gtgaccgttc acggtgactc tgcagtggca gggcaaggtg tagtgcaaga aaccttaaat
     2041 atggcaaatg ttcgtggcta taaagtagga ggcactatcc gtattgtgat caacaaccaa
     2101 atcggattta ccacctctaa cccaaatgac acccgctcca ccgaattttg taccgacatt
     2161 gccaaaatga ttcaggcacc gattattcac gttaatggtg atgatccgga agctgttgcc
     2221 tttgcagcga gaatggctgt ggaatatcgt actaaattta aacgtgatat ttttattgat
     2281 ttaatctctt accgtcgtca cggtcataat gaagccgatg agcctttagc aactcagcca
     2341 atgatgtata gcatcatcaa aaaacacccg acacctcgca aagtctatgc ggatcgttta
     2401 attaccgaag gtgttttaac caacgagcaa gaaatcgaga tgttaaacct gtatcgtgat
     2461 gccttagaca acggcgaatg cgttgtgccg gaatggcgtg aaatggatat ggcagcggtc
     2521 gattggctac aatatctcaa ttacgactgg actgccctat acgaaagtaa attccctgaa
     2581 gaacgattct taaccattgc caaacgggtg tgcgaatatc cggcttcgct taaagctcat
     2641 tctcgggttg aaaagatcta cactgatcgc aaagagatgt atgaaggcaa gaaattattc
     2701 gactggggta tggctgaaac aatggcatac gctactttgt tagacgaagg cacacatgtt
     2761 cgcctttcag gagaagatgc cggacgtggc actttcttcc atcgccatgc ggtaatccat
     2821 aaccaaaatg atggaacagg ctatgtgcca cttactcaac tgcacgccaa tcaagggcgt
     2881 ttccaagtat gggattccgt actctctgaa gaagcggtac tcgcctttga atatggcttt
     2941 gccactaccg acccgaaaac actgaccatt tgggaagctc aatttggaga tttcgctaac
     3001 ggagctcaaa ttgttatcga ccaatttatc agctcaggcg aacaaaaatg gggcagaatg
     3061 tgcggtttag tgatgttatt accacacggc tatgaaggtc aagggccgga acactcctct
     3121 gcccgagtgg aacgctattt acagctttgt gctcagcaaa atattcaagt ttgcgtgcca
     3181 tcaacaccgg cacaggttta ccatatgtta cgccgtcaag cgatccgcaa aatgcgtcgc
     3241 ccattggttg caatcacacc aaaatcattg ctacgccacc cacttgcggt gtcatctctt
     3301 gatgaattga ttaacggttc attccaaaat gtgattggcg agctcgataa tatcgatcct
     3361 aaacaagtca aacgtgtggt gatgtgttcg ggtaaagtgt actacgactt aattgaaaaa
     3421 cgccgtgaaa ataaccaaac ggacgttgcc attattcgta tcgaacagct ctacccgttc
     3481 ccacttgaag aaatgcaaca aattctcgag ccttacgctc acgttaaaga ctttgtctgg
     3541 tgtcaagaag agccactaaa ccaaggtgcg tggtattgca gcaagcacaa ttttgaggct
     3601 tctctgccgg aagatgcaaa attaacttac gcaggcagac cggcttctgc ttcaccggcg
     3661 gtgggttatc catcgttaca cgcaaaacaa caaaaacaac ttgttgaaga tgctttaacg
     3721 atcgctgaat aataaaatgg gcgtatatac gccctacaag cggttcactt ttaaccaaat
     3781 tttacaattt acgctgtcgg agttttaaaa ctctctcgaa aaaaggaata agttatgacc
     3841 attgaaattt taacccccga tttacccgaa tctgttgccg atgcgaccgt tgccacttgg
     3901 cataaaaaag tgggcgacaa tgtaaaacgt gatgaaattt tagtcgaaat tgaaacagac
     3961 aaagtcgttt tagaagtacc ggcttcaagc gatggtattc ttgcagaaat tacccaagaa
     4021 caaggtgcta ctgttgtatc caagcaatct ctgggtaaat tagtagtagc taaagccggt
     4081 gatattagct ctgcaactat tgagcaaaaa acagaatcca cgccatcaga ccgcaaacac
     4141 gcagcaattg aaaacagcca cgcgaacgct gatgatcaag ggcctgcgat tcgccgttta
     4201 ttagccgaac acgacttaca agccgctgat attcaaggca gtggtgtagg cggcagaatc
     4261 acccgcgaag atattgaaaa agagattgcc aaacgagtgc aaatcaacca agtaaaaggg
     4321 tctgcaacca gccaaaacac cgttagcact gtagcattta acagccgtag cgaaaaacgt
     4381 gtgccgatga cacgactccg caaacgtatt gccgaacgct tactagaggc aaaaaacacc
     4441 actgcaatgc tcactacttt taacgagctg gatatgcaac caattatgaa tttacgcaaa
     4501 acctatggcg aaaaattcga aaaacaacac ggtgtgcgtt taggctttat gtcgttctac
     4561 attaaagccg tggttgaagc attaaaacgc taccctgaaa tcaatgcctc cattgatggc
     4621 gatgatgtgg tttatcataa ctatttcgac atcagcattg cggtttctac cccacgaggc
     4681 ttagtcacac ctgtgttgcg tgattgcgac aaaatgagta tggcagatat tgagaagaaa
     4741 attaaagaac ttgccgaaaa agggcgtgac ggcaaattaa ccgttgaaga cttaaccggc
     4801 ggtaacttta ccatcaccaa cggcggggta ttcggttcat taatgtccac cccaattatc
     4861 aacccgccac aaagtgcgat tttgggtatg cacgctatta aagaccgccc tgtcgccatt
     4921 gacggacaag tagtgatccg cccgatgatg tacctagcct taagctatga ccaccgttta
     4981 attgacggta aagaatctgt cggtttctta gtggcagtta aagagttatt agaagaccca
     5041 acccgtcttc ttttggaaat ttaatcgaaa atatgaccgc ttgtactttt agctacaagc
     5101 ggtctgtttt taataaaatt tgacaaaatc cgataaacgc tctatttgag ttcccttctg
     5161 agccacctga gcgactttga atttgtagga aaaatttagt gtttgagctt acaaggcaag
     5221 cgagttataa atttttccgt caagcaaatt caaactaagc gaagaaagtg gcgaagcagg
     5281 gtgtgctttc ttttggttac ttttctttgc acaagcaaag aaaagtaaca taaaaaaaac
     5341 caaacgagga aactctatga atctacacga atatcaagca aaacaaatat tcaaacaata
     5401 cgggcttccc gtaagccaag ggattgcttg caaaacggca gaggaagccg ttgaggcaat
     5461 taaacaacta caaggtgaac aatgggcgat aaagtgccaa gttcacgctg gtgggcgtgg
     5521 caaagccggc ggggtgaaac tggttcgcaa tgaagccgaa gtgcgtgaat ttgccgataa
     5581 atggctcggc aaacgcttag ttaccttcca aaccgatgct aatgggcaac cggttaatac
     5641 cttatattta gaagaaacct gcaatatcga ccgtgaattg tacttaggtg cagtggttga
     5701 tcgctccgct caaaaagtga tttttatggc atcaagtgcc ggtggtatga atattgaaga
     5761 agtagcggag aaaacccctg aattaattca caaagcggta attgacccac tcaccggtgg
     5821 gcagggttat caagggcgtg aattagcatt taaattaggc ttgaaaggtg atgaagttaa
     5881 acaatttgcg gatattttcg tgaaactggc taacctattt gttgaaaaag atttagcttt
     5941 attagaggta aatccacttg tcatcactaa agaagggcaa ctactctgct tagatgcaaa
     6001 aatgtcgatt gacagcaatg ctctctaccg ccacccagaa cttaaagagt tgcaagaccc
     6061 atcgcaagat gacgaacgtg aagctgaagc ggaaaaatgg aatctcaact acgttgcact
     6121 agatggcaat atcggctgta tggtaaacgg tgcggggctg gcaatgggga cgatggatat
     6181 cgtcaaactt tatggtggta aaccggctaa cttcttagac gttggcggtg gtgcaaccaa
     6241 agagcgtgta gcagcagctt ttaaattgat tttatctgat ccgaatgtga aagccgtgtt
     6301 agtcaatatt tttggtggta tcgtccgctg cgatttgatt gctgatggcg tcattgctgc
     6361 tgtaaacgaa gtcggcgtga atgtgcctgt tattgtgcgt ttagaaggca ctaatgccgc
     6421 acaggggcgt gagattttag ccaatagcgg gctgagtatt ctagcggcat catcattaaa
     6481 acaagcggct gaattagcgg taaaatctgc aaattaggag gaaaaatgtc gattttaatt
     6541 gataaaaaca caaaagtaat ttgccaaggt ttcaccggcg gacaaggtac tttccacagc
     6601 gaacaagccc ttgcttatgg cacacaatta gtcggcggag tttcgccgaa taaaggtggc
     6661 acaacccatt taggcttacc ggttttcaac accgtacgcg aagcggtaga agcaaccggt
     6721 gcaactgcaa ccgttatcta cgttccggct cccggctgta aagatgctat tttagaagcc
     6781 attgatgccg gcatccaact aattgtctgt attactgaag gcattccaac cttggatatg
     6841 ctacaagtta aacaacgtct aaaccaaacc ggcgtgcgaa tgatcggtcc gaactgcccg
     6901 ggtgtgatta cccctgatga gtgcaaaatc ggcattatgc cgggcaatat ccacaaaaaa
     6961 ggcaatatcg gtattgtttc tcgttcgggt actttaacct atgaagcagt taaacaaaca
     7021 actgacgaag gctttggtca atctacttgt gtcggtatcg gtggtgatcc gattccggga
     7081 tctaacttta ttgacatttt aaaactgttc caagacgatc ctgaaactga agcaattgtg
     7141 atgattggtg aaatcggcgg ctctgccgaa gaagaagcgg cagctttcat caaagaaaat
     7201 gtcaccaagc ctgtagtcag ctatattgcc ggtgtcaccg caccaaaagg caaacgaatg
     7261 ggacacgccg gtgcgattat cagcggcggc aaaggaaccg ccgatgaaaa attcaaaatc
     7321 cttgaagatg ccggtgtgac aaccgtcaga agtttagccg aaatcggttc agctcttaga
     7381 aaaattttag gctaaactaa aagttcaagc agtcatattt acccattttt ttgtaaatat
     7441 gaccgcttgt ttttttgcta ttaaattact ctttcgattt ccaaattaat gggtcaacac
     7501 ctttatggat aaggtcttga tgttcttcaa ttctaaacac tggctctttg ccacttttca
     7561 cttggcgaag atagtctttt aaaataatcc agacaatcgg agcaagaatt aagatagcca
     7621 ccaagttaat taccgccatt gatgccatca ctgtatcggc aaatgcccat acaatccctc
     7681 cgtctcttac tgcaccgaag tacacaaaga ataaaacgat ggcacggaat ccccacacaa
     7741 accacggact gtttttaatg tagcggatat tagtttctgc ataagcatag ttaccgataa
     7801 tggatgtgaa gcaaaacagt aaaatcacaa atgctaaaaa gtgtaaacca aattcaccta
     7861 aatggaattg taacgcactt tgagtaagtg aaatacctct taaagtttca ccaccatagt
     7921 tatttgacat aagaatgatc actgcagtac aagtacagac tacaattgta tctacaaata
     7981 cgcccaacat ttgaattaag ccttgacttg ccgggtgttt cgcatcagaa gtcgctgcag
     8041 aatttggtgc agatcccata ccagcttcgt ttgagaataa gccacgttta atccccatta
     8101 gcatagtttt ggataccaat gctccaaacg ttccacctgc agcagcagaa aaatcaaagg
     8161 cgctttcaat aattaaagag attatacttg gcactttttc aatattcata cctaaaataa
     8221 ttactgcaat aatcaagtag aataatgcca tcattggaac aacttgggca gaaactttac
     8281 caatacgctt gacccctcca gaaataatta accctgtaat caccactaat gtcatgccta
     8341 cgtagactgg gttccaatcc cacgcatttt tggttgcttc cacaatagag ttagattgca
     8401 cagcattaaa agcaaaacca aaggtgaaga taagcgtcac tgcaaaagca atagcaagag
     8461 aaggcagttt taaaccacga gtgatgtaat aggctggccc accacgaaac attccgtttg
     8521 gatctttggt tttatacaac tgagccagtg aagactcagc aaaggcggaa ctcattccca
     8581 ttaaggcggt aatccacatc cagaataccg cacccggccc acccaaagcg attgcagtag
     8641 ctaccccacc aatattacca accccaacac ggctggctaa tccagtagta aaggcttgga
     8701 acggagttaa tgatttccct tccgtagaac gtcctcccca catttcacgt aaactttgcg
     8761 gaagaagacg cagctggaca aaacctgttg taagagtgaa aaataaccct gcaccgagca
     8821 ataggataat cgttacatcc caaagtggac cgtcgattgt gttcacaatc caagtcagta
     8881 cactctcaaa aaatagaata attgaatcca taaagacctc ttaattgaaa ataaaacttc
     8941 cacattgtaa ccgatttaat atttttaaaa agttttttta atacttttaa taaaaatttt
     9001 taataactta ttaatttagc gtttgaaaaa aacgcctgta ccagcatcaa tgaaactact
     9061 gtgaaataat accattccaa atagaattaa tgcgagtcca agtgtaaatt ttaatatcca
     9121 aacgatattt cgattaattt gagctgaaaa ataccattgg ctaaccttaa ctgccttatt
     9181 tctcgcaatg ataacaagat aagcaaataa gcttaaagtc agccctgttc ctactgccat
     9241 agctaatgct gaaataaccc cccaagcata gagattgagc gtataagaaa gaaataaaac
     9301 aagaatagcg ccagagcaag gtcttgagcc aatacttaat accaacatca gctgtgcttt
     9361 ccaatctctc acttgttgca tttcatttga agtcggtaaa tgtttatgcc tacagctaca
     9421 attttcggta tgaatatgcc ctttctgcgt gacaagcggt aattttccag cttttttttg
     9481 cgaaatacga atggatttaa tgataggctt tcgcgctctg ttcaccgttc gatacacctg
     9541 gtaacaccag taaatgccga acaagatcat caaaccaaag ctcccccgct caacccattt
     9601 tagggttaaa ttaaaatatt ggcgagagag agtaaaaatc accacaatca ccgtcaccaa
     9661 tgaaattgcc actaagccct gcaccaatgc tgaggcaaaa gtgattttca tcgcctgatt
     9721 taattttgtt ttttcaaaag aaagataact gctcagaata aattttccgt gaccaggccc
     9781 aactgcgtga aatactccat ataaaaaact aataaatact aaggtaatac cggctttact
     9841 ttgattttca gctaattcat tgagcgaagc ggaaagctgt aaattaaagt ttttttgcca
     9901 ttccatcact ttaaacaata tcattggata aagctgataa agagcaaata ggctcaaaaa
     9961 tcctatcact atcattcccc aatatgttgc ttttaatcgc atataatctc cactttttga
    10021 gcaaattggg caccaagaga gtaatctcca ttttcaggca tatccagtga ttggttttta
    10081 tccaaaccgg aagcatataa gcgtaactta ctatctactt gaggttgcac caatgttgct
    10141 ttacaacttg cattactggt attgacatca gcagggcgat tatattccat cgcaatataa
    10201 taactcggtt cataagtata gaatctcact ttactctgct ttaattcgtg aggtttagct
    10261 aaatgaaaag taatataaaa aatcactctg ttattcttga tttcaaagcg agtcgctgtg
    10321 ggctttgtcg taaattttat cggctgattt tgactatcat ataaggtgct aaaataatga
    10381 ttttgcaatg cggtttgcac catttctgca gtgatattct gctgagtttt ttctttatct
    10441 tggctatttt tcacttcata aattaactca gaagaagcaa tttcatctag catccattcc
    10501 atttggaaag atttcaacat tgagccttcc acaataacat tcgttttcaa atcaacaaat
    10561 gaatgtgggt gagaaaaaac tggcattgaa tataatgcaa ttaacgtgat aatcgtctta
    10621 ataaatttca tatttacttt cactataaaa taaaaaagcg aacaagttcc ccctgttcgc
    10681 atttcaagaa tttagtgagt tggtcgataa gccgagttct gtcgtggaca atcattcctc
    10741 tagacgtaca tttacacata cgctcaagca acctacccga actctcagcg ggccaccgat
    10801 tgagttccta tttggtcttg ctacgagtgg agtttaccct gctgtggaat gttaccaccc
    10861 acacggtgcg ctcttaccgc accctttcac ccttaccgac aaagccggcg gtctgctctc
    10921 tgttgcactg gtcgtaggct cacgcccccc ggacgttatc cggcactctg ccctgtgtag
    10981 ctcggacttt cctctcgttt acttgtcccc cagtttacgt tttcacgaat ttagaggctt
    11041 caataaacca gcgattgcct aaccaactca gcactagagt ataatgcttt cgcaaaaaat
    11101 tgcaaggaaa taaccgcttg tagttattat ggcaaaataa aaaaggatta agttcattta
    11161 aaaacttaat cctcttcgct ttaattcact cgtaaaaatt acgcacgttt gaagtctaag
    11221 tgaactaatt ttggtttctc tattttatta caataatttc aaagggttat ttaatcaatt
    11281 tcctttctat ttccgaaact atatacattt ttccgaaact caaattttaa tcagattata
    11341 aaacaagcta ttttcctttt ttctcattcc aaaaatacca tccaaaacat cttaccgcca
    11401 accaagtgct ccacgcaaaa ataggtctat tttgcgcaat caaacaatcc cttaaccgct
    11461 tatctgcttc cgctctactg acttttccgt ttacgcccac gatcgtgttg atgacagcac
    11521 gaattaatat tttcaggggc gtgtaccgca atcaatttta attataacca attgttttta
    11581 aattaaatta ttgaattaca tttaaaatac acacaaaaac acccacaata aagcaatacc
    11641 tcagcactcg aagagcaaga tcgtctttga gggatagggg gtaaaataaa ttttttaaaa
    11701 atttgcaaaa aaatttaaaa aaactcttgc aataaaatta ttatgcgcct ataattaggc
    11761 tcataaagca aggctaggga gccagctaat aaccatcagc caaagggggc taaaatgaca
    11821 actcaaatta caaccgcaca aatcaacgac ttattaaaat ctcacgatat ccaaggctac
    11881 gccaacgaat ggcaaggtcg ccgcatttac attaaccttt cagcaaaaaa taacgaaagc
    11941 aaaggtgatc ggaattacca gttgtacatt gacctagcca caggcgagtt agtgagcaaa
    12001 cttggcaaag gattaacaac cagagcgttt gatgctgccg taaaagcttt tgaaaacgct
    12061 tttaatgcta aagacaccgc tgaaaataca gcgccggtag aaagcaaaga aacagagcaa
    12121 gagaacaaac aagaaacaac taagcttgta atcacaaaac caacattaaa aggctctgaa
    12181 aaacaaattg attcggcaaa cgaaatcatc aatcttttta ttgcgaagat tggtgatacc
    12241 tctatcccag taacagcaac gcaagaacaa attactcaag ctcaagcaat ggtagataat
    12301 ttttttgcaa tgacaaacgc tggaaagtgg atcgaaaaat ttaaaggtct aaatgataat
    12361 agcgaaccaa agcaattttg gttatccgta aaatatggat ggtagattga gataacaaat
    12421 aagccgtaag agcaaaggct tacggctaaa acaaacagat aactggagat tattatgact
    12481 acacaagtaa caccacaaat tatgcgcatt atcgggcaaa ttgtcgctgc tacttatggt
    12541 gacgatgtgc caacaaacgt acagacaata atcttgagat accctataag aggcataggg
    12601 tttatctcta gtcgcagaga gctatctatt aacaatggag agatagcaag attaatggat
    12661 aaaataccag gcgacctcga ggaccctaaa gacgggatgc cttttgattg tcagggggcg
    12721 ttttggcttg gatactatca atactgcaaa ttaagtaacg atgtaaaaaa ttacacatca
    12781 aaagagctat ccatcattgg cgagtcattg tatggcactc aatggcagtc taatctagcg
    12841 agagatttga gattatctga tgctcgccgt gtccgcgaat gggtggctgg tgaacgtaaa
    12901 atccctttcg gggtttgggc tgatttgacc gaattggtta aagccaaaaa agctaactta
    12961 tcatcaattc ttaaaaaatt aacaatagat tagcactgat tttaaagccc ctctcggggc
    13021 ttaattatag aaaataaaat cacaatggct aattttgcca aatttgcctg ttaaaacgtg
    13081 cgaaaaatta caaaaaacgg ctagagctat agccgttttg agtgcagatg ttaacagagt
    13141 taagctctag cacgtcctgg gccttgatac tgcacaccat catttaaatg atcttgattt
    13201 tcggttgctg ctacagcaga ttctgatctt acatttaaac tttgtgactg ttgtaatgca
    13261 tcaataagtt tatttaccac tgttccgagt gttttaattt gctcggtttg ctcattaata
    13321 gtctgcttta actcttgcag ttttaatgat tgctcatcca caaccactaa tcgacggtta
    13381 atctctccta cgtctttacc taattgataa aatgcttctg caagtgggtg tttgtgttta
    13441 gttttagtca tcgttaagct cctttgcttg ttggtatata gttaaaaagt caggtgtgtc
    13501 accatactcc tcacctatat ggataactac cagtttgtct atggtgatat caatagtttc
    13561 ggttggatgt atagtgatta agatattgtc catattcccg ccttaaattt gggtttggtc
    13621 agggataagt tggatcgtgc cgtagtactg ccattttggg tggatagctc aatttgaggc
    13681 tctgtgctgc cctctactat ggcgtgtaaa tccagcctaa taccctctaa atttacaggc
    13741 tcgccatttg tttgcctaaa aaaaaaggcg ataacttacg tcatcgcctc tgaaaaactt
    13801 aaatttatgc ttttgcattt agacccctac actacaatct gaccggtttc tttgaggtgg
    13861 ttgtagatac gtcctaacat aatctgcgtt ggcgttttgc caatatcctc ttttgttagc
    13921 ggtgcatcag taatcgcctt ggcggactgt gcatcaatgt atttgtactc acttgtgatg
    13981 attacaaagt ttgttacttc tccatcggta tcatcgcctg taccgaccac atatttagca
    14041 ttgagcgagc cgtcatcttg cgttgagtag cttgcgatgg tggaatacat tgggtttaag
    14101 attttgttaa aagttgtcat aatttttcct tttatcgata gttgaaataa aaacaatcgc
    14161 ctgaattgac ctgcaagcgg ttggttttgg atatttaaaa aagctgtggc tatccgcact
    14221 cagtactaca ttgaggcgat aactaactga tagagatata tttagccgtc gaaatggaat
    14281 aggctaaaac aataaaatct aaagagctac cacttgcatt tctatttaat gatttctcta
    14341 ttgtttttgt gattgattga tttgcttcta aaatgaatga ctcacttccg atgatttcaa
    14401 tatacctcct tgtcggcgct gcatttatgg tgacagttgc tttatagata tattcgtgtc
    14461 tataaattgg agaggaggag ttttctccat gttcttgccc ctcataaatg cgattacgat
    14521 gtgaatattt taatttgcct gtaacctgct ctataacacc tccccctatt aattgagata
    14581 cttcaagttc gccggtaaat ttgccagtag ccccctccaa tcttgcaccc tttattgtgc
    14641 cgccagagat agttgtaccg ctaattgttg taccattaat acttccgcca ctaattgtat
    14701 taccagtaat tgtcgtacct gtaattgtac cagcagttac cttaccaaga tttgccgata
    14761 ttgccgctaa attgtttaca tttagcttat ccgcagtgat cgaactcccc acaatatgac
    14821 ttgcattaat cgagtttgct gccatatttc gagctgcaat cgtgccggtt gcgatttcgt
    14881 tggcggtaat ggtatttgct gcaatttgtt gagcagtaac ggtattagta actattgatc
    14941 caccgtggat agcggttaca ccggcatttt gccaagggct aggctgagtg atatctgtaa
    15001 cattatcact agcctcctca agcattggcc tacgagcaaa taaaaatgga ttagctttac
    15061 cttgaatatt atctaatcgt aatgcgaaac gaatacagcc tgaagtaggt gcagtaaata
    15121 caaccttaac ccgtttcata tttttaaatt cgccttgatt agcactatag ccagtaatta
    15181 cctcgctgtc gcctatccaa cctaagtaag caccaccagg tttttcgata ttctcaatga
    15241 ctaatttagc ttgcccacga tgacaagcta accatacgga ggcaatatat tttttattag
    15301 gcacaacatt aatatcctga gataaccacc caattcgccc agtatcagag gtttcctttg
    15361 tagtgctata ccgcatcgaa aatactaact catttggtag tcctccattt tttagtccgt
    15421 aattatcttg ttcattccta ttgaaaatat tacgggtgtc aatatttagc cgatcaccaa
    15481 cttctcctcg ccagtagttc cagccaaacg gagctattga caaatcgggt gttgcaaaaa
    15541 taggattaac cagcaaattc ccacctagcc caatcgctaa cttatccgcc gatatttgcc
    15601 ctgctgccag gtgttccgta cggatagctc ccgccgtcaa tattccggct cttactgagt
    15661 ctgcatcaat gtggctacct ttaattgtac ctgttgcaat taaattccca ttaatcgcaa
    15721 ctttattttg agccacacca aacaatcgta ccggttggcc gtcttgagca ttttttacta
    15781 cctcaaattt gtctgccatt acaatgacag aggattctgc attgcgattg tctgccgcgg
    15841 caccaagtgc gataccggca atcgctttac gtccaccggc aatggtttcg gttttgatgg
    15901 tatgggtagc agataatttg ccgtccacac cggctacagc attactcact tgagtaattt
    15961 ttgccaagtt tgagccaaca gaagcagtaa cggcatcaat acgagagctt aatgcagagt
    16021 cggcattcgc taaagtctcc tgaat
//
LOCUS       DANLXF010000039        16041 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.039, whole genome shotgun sequence.
ACCESSION   DANLXF010000039 DANLXF010000000
VERSION     DANLXF010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16041
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.039"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..1551
                     /locus_tag="PXD66_000751"
     CDS             <1..1551
                     /locus_tag="PXD66_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsI"
                     /protein_id="HDL6319075.1"
                     /translation="TQLAQVTGLDYNSLIEKVRNNPSSVFLRLDNENLVRERQKFQAL
                     SKEIGRSYNDIMDELYSKNRQRFLYLSRHQSEAISEYAKELDIDALVIKVESRRFYPL
                     AEESSQLIGFTDKDDMHGSEGLERSFDSLLIGKNGKQIIRKDAKGNIVENIRSEKQYD
                     PQDVMLSIDEELQSMVYGEIKKAVQENNAESGTAVLVDVQTGEILAMANAPSFNPNKR
                     DSFKPELMRNRAITDTFEPGSTVKPFTVLTALQNGVTYRDEVIGTRPFVVNGHTIKDV
                     APRDSLTLTGILQKSSNIGVSKLALRMPANALVDTYTKVGFGKDTGLGLGEQRGTNGD
                     RKRWADIERATLSYGYGLNVTPLQLARAYATLGSFGLYRPLSITKVDPPVIGERVLPE
                     KTTRDVVHMMESVAAKGEGGHRAAVDGYRVAIKTGTARKLEKGKYVEKYIAYTAGLAP
                     ASDPRFALVVLINEPKAGNYYGGFVSAPLFSRIMGYTLKQRNIKPDNLTENTQSAVRE
                     IKLERPIN"
     gene            1560..3050
                     /gene="murE"
                     /locus_tag="PXD66_000752"
     CDS             1560..3050
                     /gene="murE"
                     /locus_tag="PXD66_000752"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HDL6319076.1"
                     /translation="MKRLLPFLTELDAWVEELTELKQMTLDSRQVQAGDLFVALKGHQ
                     VDGRKFIPNAIEQGAAIILAEADEDQPEIELDPKFAKFNLDRTACCKVVSVPRLPKLL
                     SEIAGALYHNPSHKLTLAGITGTNGKTTTAQLLAQWHNLLGGKSAVMGTIGNGLYGQV
                     QEAVNTTGSAIEIQHNLANFVELGADFCAMEVSSHGLAQFRAEALDFDLAIFTNLSRD
                     HLDYHNTMEEYAQAKFRLFNELSTKAQVINADDEIGREWLTQLPNAVAVSTDPKFAGN
                     HQFVKATAVKFTLQGASIAFESSWGNGELHSRLIGAFNVNNLLTAFAGLLALGFDIQE
                     LVKTAPNLVGVAGRMECITAPNKPMVIVDYAHTPDALEKALQAARLHCEGELWCIFGC
                     GGDRDAGKRPLMATIAEKLTDKVIATDDNPRTEDNQKIMADIVKGFSKPQQIIHNREE
                     AIKTAIEQAKATDVILIAGKGHEDYQIIGTEKLHFSDQETARKYLF"
     gene            3138..4532
                     /gene="murF"
                     /locus_tag="PXD66_000753"
     CDS             3138..4532
                     /gene="murF"
                     /locus_tag="PXD66_000753"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDL6319077.1"
                     /translation="MIKLTTQQIAEILNAKLIGNGETVVETTSTDTRQAVENGLFFAL
                     KGENFDAHHYLQNAVAQGCVAVVVERECEIDVPQLVVADTRLALGELAKWLKAKLNPK
                     TVAMTGSSGKTTVKEMTAKILQKMTACEQEVLYTFGNLNNDLGVPMTLLRLTEQHKYA
                     VIELGANHIGEIAYTTAIAQPDACLVNNVAAAHLEGFGSLAGVAQAKGEIYRGLKENG
                     KAIVNLAFYYPQWQKEIGSREMQSFSYIGSDSDSYADYWAEDVELHLSGSRFTLHTPQ
                     GEIAIDLPYLGKHNVSNALAATSLAMSVGATLEAVKAGLEQRSMVKGRLYPVQINDHC
                     LFIDDTYNANVDSMKSAISVLENYPAYRIFAVGDMAELGEESSKCHQEVADFAKNAAL
                     DLIVSFGKESAVISQQAEHHFTDKQQMVDFLLPIILQKIEQKQPLVLLAKGSRSQKME
                     TVISELCKPFNLNF"
     gene            4553..5635
                     /gene="mraY"
                     /locus_tag="PXD66_000754"
     CDS             4553..5635
                     /gene="mraY"
                     /locus_tag="PXD66_000754"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDL6319078.1"
                     /translation="MLVWLAELLVQYNTAFNVVSYITFRAIMALLTAMGIGLWIGPKV
                     IRRLQILKFGQEVRNDGPESHFKKRGTPTMGGVMILAAIGVSTLLWADLRNSYVWFTL
                     FVLFGYGAVGFVDDYRKISRKNTDGLIARWKYFWLSAIALVAVFGMYAVGKDTAATQL
                     VVPFFKDVMPQLGLFYIVLSYFVIVGTSNAVNLTDGLDGLAIVPTIMVASAFALIAWA
                     TGNFNFAQYLHIPFIPNAGELVILCTAIVGAGLGFLWYNTYPAQVFMGDVGSLSLGGA
                     LGVIAVLVRQELLLVVMGGVFVVEALSVILQVGSYKLRQKRIFRMAPIHHHFELKGWS
                     EPRVIVRFWIITLMLVLVGLVTLKLR"
     gene            5699..7000
                     /gene="murD"
                     /locus_tag="PXD66_000755"
     CDS             5699..7000
                     /gene="murD"
                     /locus_tag="PXD66_000755"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDL6319079.1"
                     /translation="MQKQYENKTVTIIGLGTTGLSCVDFFKKKNANIQVIDTRANPAG
                     ADKLDVTIPLHTGGLNSEWLLKSDLIVISPGLAVATPEIQQAIQAGVEIVGDVELFCR
                     EAKAPIIAITGSNGKSTVTTLTTEMAKQAGIKVGMGGNIGVPVLSLLEGDYDLFVLEL
                     SSFQLETTYSLKAKAATVLNISEDHMDRYDSIADYRQAKLRIYDNAENIIVNGEDAQT
                     YPIQAVKKVIRFAEQNAEYSLRNQMLYSGDDAIIQTKQMLISGRHNEMNALAAMALAE
                     AANVPREGIVKALQIYGGLPHRFQAVATNDGVRWVNDSKATNVGSTVAALNGLNIAGT
                     LYLLLGGDGKAADFSELKPLINKPNIVCYCFGQDGAALAELSEQSVLVETMQQAIEAI
                     RPQLKKGDMVLLSPACASLDQFKNFEQRGEVFMALAQGQHA"
     gene            7000..8178
                     /gene="ftsW"
                     /locus_tag="PXD66_000756"
     CDS             7000..8178
                     /gene="ftsW"
                     /locus_tag="PXD66_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HDL6319080.1"
                     /translation="MLERIKSEWDKWVRLTPSNSLYDRTLIWLFLGLLIIGFVMVTSA
                     SLPVSTRLNNDPFYFAIRDGLYIIASIIFCYVFVQIPIEKWEKHNLALFFISIGFLIA
                     VLIFGRSINGAVRWIPLGILNFQPAELAKLAVICYFASFYVRKYDEIRKEKASFWRPA
                     VILFLFGFLLILQPDLGSTFVLFVLTFSMLFIVGAKIMQFMFLGVVGTVLFAVLILTS
                     EYRLKRVTSFMDPFADAYGDGFQLSNSQMAFGQGEFWGQGLGNSVQKLEYLPEAHTDF
                     VMAVIGEEFGFFGIACIVLLLILLTVRALKISKESLVLEERFKGYMAFGIAIWVFLQG
                     FVNLGVASGLLPTKGLTFPLVSYGGSSLVIMSIAIAVLLRIDHENRAERIGHAKIKTI
                     "
     gene            8297..9352
                     /gene="murG"
                     /locus_tag="PXD66_000757"
     CDS             8297..9352
                     /gene="murG"
                     /locus_tag="PXD66_000757"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDL6319081.1"
                     /translation="MAKKLLVMAGGTGGHVFPAIAVARELQKQGWQIRWLGTKDRMEA
                     TLVPKHGIEIDFIEISGLRGKGVAALLKAPFAIFKAVMQARKIIKNYQPDAVLGMGGY
                     VSGPGGIAAKLCGVPVILHEQNAVAGLTNVWLSKIARKVLQAFPTAFKEAEVVGNPVR
                     EDLSALPLPNERFAKRGYPINILVMGGSQGARVINQTVPEVAKVLGNNVFISHQVGKG
                     NLAGIEEVYQQTGNGIASEFIDDMKAAYEWADLVICRSGALTVCEIAAVGLPAVFVPF
                     QHKDRQQFLNANYLAADGAAVIIEQSDFTPKALQQALEPLIANRQLLLDMSMRARAKS
                     TPMAAKRVAEVIIQEAN"
     gene            9428..10855
                     /gene="murC"
                     /locus_tag="PXD66_000758"
     CDS             9428..10855
                     /gene="murC"
                     /locus_tag="PXD66_000758"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013527829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDL6319082.1"
                     /translation="MKNFQDKVKKLVPEMRRVSQIHFIGIGGAGMSGIAEVLLNEGYQ
                     ISGSDIADRAVTQRLSAAGAKVFIGHQAENIAGASVVVVSTAIDETNPEIIAAREARI
                     PVIRRAEMLAEIMRFRHGIAIAGTHGKTTTTAMISMIYTEAKLDPTFVNGGLVKSTGK
                     NAHLGASRYLIAEADESDASFLHLQPMVSVVTNIEPDHMDTYGGDFEKMKETYVRFLR
                     NLPFYGLAVMCADDETVMEIAPRVGRQVITYGFSENADYRIEDYQQTGFQEHYTVVCP
                     SGERIDVLLNVPGMHNALNATAALAVAKEEGIGNEAILAALADFQGAGRRFDQLGSFI
                     RPNGKIMLVDDYGHHPTEVDVTIQAARSGWEGKRVVMIFQPHRYSRTRDLFDEFVQVL
                     SKVDALILLDVYAAGEAPIVGADSKALARSIRNLGKVDPILVSDTDQLGEVLDQIIQD
                     GDLILAQGAGSVSRISRGLAESWKN"
     gene            10945..11856
                     /locus_tag="PXD66_000759"
     CDS             10945..11856
                     /locus_tag="PXD66_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDL6319083.1"
                     /translation="MSIKNEKIAVLFGGVSQEREVSLNSGAAVTAALKSLGYNVEGID
                     TKEFPIEKLKEKGIQRVFNILHGGIGENGVLQGALEQMGIPYTGCGVMASAVTLDKFR
                     TKLLWNAVGLPTADMVVVQRGQAVDFDQIIAKLGLPVFVKSSCEGSSVGVFKVKTKEE
                     LAPAIHEALKFDSIVLVEEFLAGNEYSVPVLDGQVLPAVQVIPDGEFYDYHAKYVSDN
                     TQYVVPALNDERQAEVAKLVKAAYDVVGCRGWSRIDVMEDGEGKFRLVEVNTNPGMTS
                     HSIFPKSAATVGISFEKLVERVLELSA"
     gene            11856..12647
                     /locus_tag="PXD66_000760"
     CDS             11856..12647
                     /locus_tag="PXD66_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HDL6319084.1"
                     /translation="MGIFRKKNSTNVRLNVLNPKTPVSPRKWLVFIKPLIVLLCTLFV
                     FGVYSNWQNILESWDKTPIRAYALTHKTQFTTNADIRETLSKEPALKGYFGQNIQEVK
                     DKLLSIPWVKDVAVRKLYPDRLSITLLEHKPVAIWNDTNFVSEQGTVFSLPKDRIDKN
                     GLPLLYGPDTEGKSVLEAWNKIRADLNARNLDLTSVSVDNRGSWTIRLSNQVELRLGR
                     GEWTSKIDRFVTIFPEIHIPEGNRLAYVDLRYPHGAAVGFSPIKN"
     gene            12670..13941
                     /gene="ftsA"
                     /locus_tag="PXD66_000761"
     CDS             12670..13941
                     /gene="ftsA"
                     /locus_tag="PXD66_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDL6319085.1"
                     /translation="MTKAVESKIIVGLDIGTSKVVAVVGEVLPDGVINVMGAGVCPSK
                     GVDRGGVIDLDAAVNSIQRAIEQAESIADCQIMGVTLAISGQHIVGLNEAGSSTLSGG
                     IVTQDNIESAVDMAKAIKLQDGLETLHMIPQEYHVDRLPATKNPIGLSGMRLQVQAHL
                     IACHQAWLKNLKNAVEHAKLKVDQVVFSGLASSYSVLTEDEKELGVCLIDIGGGTMDV
                     LVYTDGALRYSKVIPFAGNNITDYLARVFTTSRPEAESLKVGYGSAISPPTHNSDKKI
                     EVAGLGGRMARTFTRAQVATVTSQCYNDLLKVVEEELTQLRHELFQKGIKQELIAGVV
                     ITGGGSQIEDIVECAKSIFGSQVRVGYPLNITGLTDYVNKPQYATALGLLQYSHYNYT
                     EQGTSTDSNEDIFAPIGRGTKKIIDFFKSKF"
     gene            13974..15221
                     /gene="ftsZ"
                     /locus_tag="PXD66_000762"
     CDS             13974..15221
                     /gene="ftsZ"
                     /locus_tag="PXD66_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDL6319086.1"
                     /translation="MFEPIIEQTQDAIIKVVGVGGGGGNAVDRMSRSADDIKGVEFFD
                     VNTDAQVLRKRTTRQTIQIGASTTKGLGAGADPMVGKQAAEEDREAIANALKGANMTF
                     IAVGMGGGTGTGAAPVVAQIAKEQGSLTVGVVTKPFRFEGPRRMRFADQGIKELSQYV
                     DSLIIIPNDKLRGLGKQTTAVDAFAAANDVLSNCVLGITNMITSSGGSTGADINVDFA
                     DVRTVMSGKGHAMIGTGFAEGEVGEGRAEKAMNDAISSPLLENVDISGASGMLINISA
                     GTDFLLEEVYAMMDLIYGFATEDAAIVFGCNYYPEMDGKVSVTLVATGIGQPEEALHM
                     PHKAQPVYAQQGNHVQATQPTQPNHAVQQPNFTQPSSYGQPNQFSGQPQPLQQPVAPK
                     PQSVDTAQIFNPNSIPGFMRNSQ"
     gene            15253..>16041
                     /gene="lpxC"
                     /locus_tag="PXD66_000763"
     CDS             15253..>16041
                     /gene="lpxC"
                     /locus_tag="PXD66_000763"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005723052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HDL6319087.1"
                     /translation="MTKQRTLKQAAKVTGIGLHSGKKVTLTLRPAPANTGVIYARTDL
                     EPVVYFPASADSIRDTQLCTCMVNDDGVRISTVEHLNAAMSALGLDNVIVEVDAPEIP
                     IMDGSSSPFIYLLLDAGIEEQDAPKKFIRIKETVRVEEGDKWAELKPYNKGFKLNFTI
                     DFEHPLIPKNVGSYSIDFSAENFIQQLSRARTFTFMKDVEYLQSIGLALGGSLDNAIV
                     LDDYRILNEDGLRFKDELVRHKMLDSIGDLYMCGYNILGEFSAYK"
BASE COUNT         4766 a         3017 c         3755 g         4503 t
ORIGIN      
        1 actcagcttg cccaagttac aggtttggat tacaacagtt taatagaaaa agtacggaat
       61 aaccctagct cggttttttt acgtttagat aacgaaaatt tagtaagaga acgccagaaa
      121 ttccaagcgt taagcaaaga aatcggccgt tcctataatg acataatgga cgagctttat
      181 agcaaaaacc gccagcgatt cctttactta tcccgtcatc aatctgaagc gatcagcgaa
      241 tatgcaaaag aactagatat tgatgcatta gtgattaaag tcgaatcacg ccgtttttac
      301 ccgctggcag aagaatcttc acagttgatc ggttttactg ataaagatga tatgcacggt
      361 tcggaagggc tggagcgtag ttttgattcc ttacttatcg gcaaaaacgg taaacaaatt
      421 atccgaaaag atgccaaagg caatattgtg gaaaatatcc gttcggagaa acagtacgat
      481 ccgcaagatg ttatgttaag cattgatgaa gaattgcagt ctatggtgta cggtgaaatc
      541 aaaaaagcgg tacaggaaaa taatgcggaa tcaggcactg cggttttggt tgatgtgcaa
      601 accggcgaaa ttttggcaat ggcaaatgcc ccttcattca acccaaacaa acgggatagt
      661 tttaaacctg aattaatgcg taaccgtgct attaccgaca cttttgaacc gggttcaacg
      721 gtgaaacctt ttaccgtatt aaccgcatta caaaatgggg taacttatcg tgatgaagtt
      781 attggcactc gcccatttgt cgtcaatgga catacaatta aagacgttgc tccaagggac
      841 agccttacgc ttaccggcat cttgcaaaaa tccagtaaca tcggggtcag taaattagcc
      901 ctcagaatgc cggcaaatgc gttagtggat acttacacga aagtcggttt cggtaaagac
      961 accggtttag gcttgggtga acaacgaggt acgaacggtg atcgcaaacg ttgggcggat
     1021 attgaacgtg ctaccctttc ctatggatac ggtttaaatg tgaccccatt acaacttgcc
     1081 cgtgcttatg cgactttagg aagtttcggg ctgtatcgcc cactttcgat taccaaggtc
     1141 gatccgccgg tgattggcga acgggtgcta ccggaaaaaa ccacccgaga tgtcgtgcat
     1201 atgatggaaa gcgttgcggc taaaggcgaa ggcggacacc gtgcagcggt ggacggctac
     1261 cgtgtagcca tcaaaaccgg tacagcacgc aaattagaaa aaggtaaata tgttgaaaaa
     1321 tatattgctt ataccgcagg gcttgccccg gcaagcgatc ctcgctttgc attagtggta
     1381 ttaatcaacg aacctaaagc aggcaactat tatggtggct ttgtttctgc accactgttc
     1441 tcacgcatta tgggttacac cttaaaacaa cgtaatatta agccggataa cttaacggaa
     1501 aatacccaaa gtgccgtgcg tgaaattaaa ttagaacgcc ctatcaatta aggttagcta
     1561 tgaaacgttt actccctttt ctgacagaac ttgatgcttg ggtggaagaa ctcaccgagc
     1621 taaaacaaat gacacttgat agtcgccaag ttcaagccgg tgatctcttt gttgctttaa
     1681 aaggacatca agtcgatggc agaaaattta ttccgaatgc gatagaacaa ggagcagcaa
     1741 ttattttggc tgaggctgat gaagatcaac cggaaattga gcttgatccg aaatttgcaa
     1801 aatttaacct agatcgcacc gcttgttgca aggttgtctc tgttccacgt ctgccaaaat
     1861 tgctctctga aattgcagga gctctttacc ataacccgtc acacaagctg actttagccg
     1921 gcattaccgg cacgaacggt aaaacgacta ctgctcaatt attagcacaa tggcataatt
     1981 tattgggtgg gaaatcggca gtaatgggta ctatcggcaa cggtttgtac ggacaagtgc
     2041 aagaagcggt aaataccacc ggctctgcca ttgaaattca gcataatctt gcgaactttg
     2101 ttgaacttgg agcggatttt tgtgcgatgg aagtcagctc tcacggtttg gctcaattta
     2161 gagccgaagc cttggatttt gatttagcga tctttacgaa tttaagccga gatcaccttg
     2221 attaccacaa cacgatggaa gagtatgccc aagccaaatt ccgtctcttc aacgaattaa
     2281 gcaccaaagc tcaagtaatt aacgccgatg atgaaatcgg gcgtgaatgg ctgacacaat
     2341 taccaaatgc agttgcggta agcaccgatc cgaagtttgc aggcaaccac caatttgtca
     2401 aagccactgc ggtaaaattt accctacaag gggcaagtat tgcgtttgaa tcaagttggg
     2461 gcaatggcga gttacatagc cgtttaatcg gggctttcaa tgtaaataat ctgcttaccg
     2521 cctttgccgg acttttagct cttggctttg atattcaaga actggttaaa accgccccga
     2581 atttagttgg cgtagcaggt cgaatggaat gcattaccgc tccaaataag ccaatggtaa
     2641 ttgtggacta tgctcacacc cctgatgctc ttgaaaaagc cctacaagcg gctcgtttac
     2701 attgcgaagg cgaactttgg tgcattttcg gctgtggtgg agaccgtgac gccggcaaac
     2761 gcccattaat ggcgacgatt gccgagaaat taaccgataa agtgattgct acagacgaca
     2821 accctcgcac cgaagataat caaaagatta tggcagatat tgtgaaaggt tttagtaaac
     2881 cccaacaaat tatccacaat cgtgaagagg caattaaaac cgccattgag caagctaaag
     2941 ccacagatgt gattctaatc gcaggcaaag gtcacgaaga ttatcagatt attggcacag
     3001 agaagttaca tttttcggat caggaaacgg caaggaaata tctgttttaa tgtcactttc
     3061 tttgcacaag caaagaaaag tgacagtgac aaatacaaaa tgctcgtaat taattttata
     3121 aagaaaaaga taaatcaatg ataaaactaa ccacccaaca aatcgctgaa atcctaaatg
     3181 ccaaattgat cggcaatggc gaaactgtgg tagaaaccac cagcaccgac acccgacaag
     3241 cggttgaaaa cggcttattt tttgcattaa agggcgagaa ctttgatgct catcactatc
     3301 ttcaaaatgc agtcgcacaa ggttgtgtag cggttgtggt tgagcgtgag tgtgaaattg
     3361 atgtaccaca acttgtggtg gcagataccc gtttggcatt gggggaattg gcgaaatggt
     3421 taaaagctaa gctgaacccg aaaacggtag cgatgacagg ttcttccggc aaaacaaccg
     3481 tcaaagagat gacggcaaaa attctgcaaa aaatgaccgc ttgcgaacag gaagtgcttt
     3541 atacttttgg taacttaaac aatgacttag gtgtaccgat gaccttgctt cgcttaaccg
     3601 aacagcataa atatgcggtc attgagctgg gggcaaatca tatcggtgaa atcgcttata
     3661 ccaccgctat cgctcagcct gatgcctgct tggtgaataa tgtggctgcg gcgcatttag
     3721 aggggtttgg ttcattagca ggcgtggcac aagccaaggg cgaaatttat cgtggcttga
     3781 aagaaaatgg caaagcgatt gtcaatttag ctttttatta tccgcaatgg caaaaagaga
     3841 ttggctcacg ggaaatgcaa tctttctctt atatcgggtc agattcggat tcttacgcag
     3901 attactgggc ggaagatgta gaactgcatt taagcggttc acgctttacg ctacataccc
     3961 cacagggcga aattgctatt gatctgcctt atttgggcaa acataacgtg agcaatgcct
     4021 tagcggcaac ttcgctggcg atgtctgttg gggcaacgct agaagcagtt aaggcagggc
     4081 ttgagcagcg ttcaatggta aaagggcgtt tgtatccggt gcaaattaac gaccactgtt
     4141 tgtttattga tgacacttac aacgccaatg tggattcaat gaaatcggcg atttctgttt
     4201 tggaaaatta tccggcttat cgtatttttg cagtgggtga tatggcggaa ttgggtgaag
     4261 aaagtagcaa atgccaccaa gaagtagcgg attttgccaa aaatgcagca ttagatttaa
     4321 tcgtgagctt tggaaaagaa agtgcggtaa tcagccaaca ggcagaacat cattttaccg
     4381 ataaacagca aatggttgat ttcctactgc cgattatttt gcaaaaaatc gaacaaaaac
     4441 aaccgcttgt attattggca aaaggctctc gtagccagaa aatggaaacc gtgatttccg
     4501 aattgtgtaa accgtttaac ctaaatttct aattattttt aaggactaag caatgttagt
     4561 ttggcttgca gaactattgg ttcaatataa cacagcgttt aacgttgtct cttatattac
     4621 tttccgtgcc attatggcgt tattaactgc aatgggaatc gggctttgga tcgggccgaa
     4681 agtaattcgc cgtttgcaga tcttaaaatt tggtcaggaa gtgcgtaatg acggaccgga
     4741 aagccatttc aaaaaacgtg gtacgccaac aatgggcgga gtgatgattc tcgcagcgat
     4801 tggggtgagt acattgcttt gggcagatct gcgtaattcc tatgtttggt tcacgctgtt
     4861 tgtgctattt ggctacggtg cagtgggctt tgttgatgat tatcgtaaga tctctcgtaa
     4921 aaataccgat ggtttgattg cccgttggaa atatttttgg ttatctgcta tcgcattagt
     4981 ggcggtgttt ggtatgtatg cggtgggtaa agataccgct gcaacacaat tagtcgtgcc
     5041 gttttttaaa gatgtgatgc cgcaactcgg tttattttat attgtgttat cctatttcgt
     5101 gattgtcggc accagtaatg cggtaaacct aacggacggc ttagacgggc ttgcgattgt
     5161 gccaactatt atggtcgcat ctgcctttgc gttaattgcg tgggcaaccg gtaactttaa
     5221 ttttgcacaa tatttacata ttccttttat tccaaatgcc ggtgaattgg ttattttatg
     5281 tactgcgatt gtgggagcag gtttagggtt cttatggtat aacacctacc ctgcacaggt
     5341 gtttatgggc gatgttggct cactttcgct cggcggtgca ttaggcgtga ttgcagtatt
     5401 agttcgccaa gaattattat tggtggtaat gggcggtgta tttgtggttg aagccctctc
     5461 ggtgatttta caagtaggtt catacaaatt acgccaaaaa cgtattttcc gaatggctcc
     5521 aattcaccac cattttgaac tcaaaggctg gtctgaacca cgggttatcg tgcgtttctg
     5581 gattatcacg ctaatgctgg ttttagtcgg cttagtaaca ttgaaattac gttaattgat
     5641 tgaataaggt gcattgagat aatgtacctt atgttatttt taaattacag aataaattat
     5701 gcaaaaacaa tacgaaaaca aaaccgttac tattatcggc ttaggtacaa cagggttatc
     5761 ttgtgtggat ttctttaaga aaaaaaatgc caatatccaa gtgattgaca cccgagcaaa
     5821 cccagccgga gcggataaat tggacgtaac tattccgttg cataccggtg ggttaaacag
     5881 tgaatggttg ctgaaatctg atcttattgt aattagccca ggtttagccg ttgccacacc
     5941 tgaaattcag caagcgattc aagctggtgt tgaaatagtc ggtgatgttg aactgttttg
     6001 tcgggaagcg aaagctccga ttattgcgat caccggttca aacggcaaaa gtaccgtaac
     6061 gactttaact accgaaatgg cgaagcaagc ggggattaaa gtgggaatgg gtggcaacat
     6121 cggtgtgccg gtattaagtt tgctggaggg cgattatgac ctttttgtgc tggagctatc
     6181 cagcttccag cttgagacaa cttattcact gaaagcaaaa gctgccactg ttttaaatat
     6241 tagcgaagat catatggatc gctacgattc gattgccgac tatcgccaag cgaaattgcg
     6301 gatttacgat aatgcggaaa atatcatcgt aaacggtgaa gatgcccaaa cttacccgat
     6361 acaagcggtc aaaaaagtga ttcgttttgc agaacaaaat gcggaatata gcctacgcaa
     6421 tcagatgctt tattcaggcg atgatgcaat tatccaaact aaacagatgc tgattagcgg
     6481 ccgtcataat gagatgaatg cgttagcggc gatggcatta gcagaagcgg cgaatgttcc
     6541 tcgtgaaggc attgtgaaag ctttgcaaat ttatggtggt ttgccacacc gtttccaagc
     6601 ggtggcaacc aatgatggcg tgcgttgggt caatgattcc aaagcgacca atgtgggtag
     6661 cacggtagcg gcattaaacg gcttgaatat tgccggtaca ctttatttac ttttaggtgg
     6721 tgacggcaag gctgcggatt tttccgaact caaaccatta attaataagc caaatattgt
     6781 ctgctattgt ttcgggcaag atggagcggc attggcagaa ttaagcgaac aaagtgtgtt
     6841 ggttgaaacg atgcagcaag caattgaagc aatccgccca caactgaaaa aaggcgatat
     6901 ggtactgctt tctccggctt gtgccagttt agatcagttt aaaaactttg agcaacgtgg
     6961 cgaggtattt atggcattag cacaaggaca gcacgcataa tgttagaaag aattaagtcg
     7021 gaatgggata aatgggtcag attaacccct tctaattcac tttatgacag aactctaatt
     7081 tggctatttt taggcctgct aattatcggt tttgtgatgg taacatctgc ttcactccca
     7141 gttagcacaa ggttgaataa cgatccattt tattttgcga ttcgagacgg gctgtatatt
     7201 attgcttcaa ttatcttttg ttatgttttt gtacaaatcc caattgaaaa atgggaaaag
     7261 cataacttgg cactgttttt tatttcaatc ggttttttaa tcgccgtact cattttcggc
     7321 cgctcaatta atggtgcagt gcgttggatt ccgttgggta ttctcaactt tcagccggca
     7381 gaattagcca aattagcggt catttgctat tttgccagtt tctatgtacg taaatatgat
     7441 gaaattcgta aagaaaaagc cagtttctgg cgaccggcag tgattttatt cttattcggc
     7501 ttcttgctga ttttgcagcc ggatttaggt agtacctttg tattatttgt tctcactttc
     7561 tcaatgcttt tcattgtggg ggcaaaaatt atgcaattta tgtttttagg cgtggtggga
     7621 acggtgttat ttgcggtgtt gattttaacc tcggaatacc gtttaaaacg tgttacctcc
     7681 tttatggatc cgtttgctga tgcttatggc gacggtttcc agctctcgaa ctcacaaatg
     7741 gcatttggac aaggtgaatt ttggggacaa ggtttaggta attcggtaca aaagctagaa
     7801 tatttgccag aggcacatac tgactttgta atggcggtta ttggtgaaga atttggtttc
     7861 tttggtattg cttgcatcgt acttttactt attctattaa cggtgagagc cttaaaaatc
     7921 agtaaagaat ctttggtgct ggaagagcgt tttaaaggct atatggcatt tgggattgcg
     7981 atttgggttt tcctacaagg cttcgtaaac ttaggggtgg catcaggttt gctgccaacg
     8041 aagggcttaa ccttcccatt agtgagttat ggtggttcga gtttggtaat tatgtcgatt
     8101 gcgattgcag ttttgctacg aattgaccac gaaaaccgtg ccgagcgaat cgggcacgca
     8161 aaaattaaaa ccatctagcc gagcggtctg caacgttaca atttgcaaaa ttttacaaaa
     8221 atgagaccgc ttgtattagc tggaaagcat aaaaattcag taaaatagca cgttttagaa
     8281 taaaaaagag ttgaaaatgg cgaagaaatt attagtaatg gcaggtggaa caggggggca
     8341 cgttttccct gcaattgccg tagctcgaga gctacaaaaa caaggctggc aaatccgctg
     8401 gctggggacg aaagaccgaa tggaagccac tcttgtgccg aaacacggta ttgagattga
     8461 ttttattgaa atctcaggct tgcggggtaa aggcgttgca gcattgttga aagcaccgtt
     8521 tgctattttt aaagcggtga tgcaagcccg caaaatcatt aaaaattatc agcctgatgc
     8581 ggtgttggga atgggcggtt atgtgtccgg tccgggcggt attgcggcta aattatgtgg
     8641 cgtgccggtg attttacacg agcaaaacgc agttgccggc ctaaccaatg tgtggctatc
     8701 caaaattgcc cgcaaggtgt tacaggcttt ccctactgct tttaaagagg ccgaagtagt
     8761 aggtaatcct gtacgtgaag atttatctgc tcttccgctg ccgaatgaac gtttcgccaa
     8821 acgaggctac ccgattaaca ttttagtaat gggtggcagc caaggggcaa gagtgatcaa
     8881 ccaaaccgtg ccggaagtgg caaaagtgtt gggtaataat gtgtttatca gccaccaagt
     8941 cggtaaaggt aatttagccg gtatcgaaga agtctatcaa caaaccggca atgggattgc
     9001 ttccgagttt attgatgata tgaaagccgc gtatgagtgg gcagatttgg tgatttgtcg
     9061 ttccggtgcg ttaacggtgt gtgaaattgc cgccgtaggt ctgcccgctg tgtttgtgcc
     9121 gttccaacat aaagacaggc aacaattttt aaacgctaac tatttggcgg cagatggagc
     9181 agcagtgatt atcgaacaga gcgatttcac tccaaaggca ttacagcaag ccttagaacc
     9241 cttaattgcc aatcgccaac tcttattgga tatgtcaatg cgagccagag caaaatccac
     9301 cccaatggca gcaaaacgtg tagcggaagt gattattcaa gaagcgaatt aacttactac
     9361 aagcggtcgt ttttggtaaa aattttgcaa aatgtaaccg cttgcgaata gattaaaagg
     9421 acaataaatg aaaaatttcc aagataaagt caaaaaatta gtgcctgaaa tgcgtcgtgt
     9481 cagccaaatt cattttatcg ggattggcgg agccggtatg agcggcattg cggaagtgtt
     9541 attaaacgag ggataccaaa tttcaggttc ggatattgca gacagagcgg ttacacaacg
     9601 tctatccgca gccggagcga aagtctttat cggacaccaa gcagaaaata ttgctggggc
     9661 gagtgtggtg gtggtttcta ctgcgattga tgaaaccaac cctgaaatta ttgccgctcg
     9721 agaagctcga attccggtga ttcgccgagc agaaatgctt gccgaaatta tgcgttttcg
     9781 ccacggcatt gcgattgcag gcacacacgg taaaaccact acaaccgcaa tgatttcaat
     9841 gatctacacc gaagcaaaat tagatccgac tttcgtgaat ggcggtttgg taaaatctac
     9901 cggcaaaaat gctcatttgg gggcgagccg ttatttaatt gccgaagccg atgagagtga
     9961 tgcttctttc ttgcacttgc agccaatggt gtcggtagta accaacattg agcctgacca
    10021 tatggacacc tatggtggcg attttgagaa aatgaaagag acttacgtac gatttttgcg
    10081 taacttacct ttctacggct tagcggtaat gtgtgccgat gatgaaacgg taatggaaat
    10141 tgctcctcgt gtcggcagac aagtgattac ctatggcttt agcgaaaatg cggattaccg
    10201 aattgaagat taccagcaaa ccggcttcca agagcattac accgtagttt gcccaagcgg
    10261 tgaacgaatt gatgtgctgt tgaatgtgcc gggtatgcac aatgccttaa atgcaaccgc
    10321 tgcgttagcg gtggcaaaag aagaagggat tggcaatgag gcgattttag ctgctttagc
    10381 tgatttccaa ggggcaggac gccgctttga ccaattaggt tcatttattc gcccgaacgg
    10441 caaaattatg ttagttgatg actacggcca ccacccaact gaagttgatg taaccattca
    10501 agccgctcgt tcaggctggg aaggtaaacg tgttgtgatg attttccaac cgcaccgcta
    10561 ttctcgtacc cgtgatttat tcgatgaatt tgtacaagta ctgtctaaag tcgatgcctt
    10621 gattttattg gatgtgtatg ctgccggcga agctccgatt gtgggggcag acagtaaagc
    10681 cttagcccgt tcaatccgta atttaggtaa ggtcgatccg attttggttt cagataccga
    10741 tcaacttggc gaggtattag atcaaattat tcaagacggt gatttaattt tggcacaagg
    10801 tgcgggcagt gtaagccgca tttctcgtgg cttggctgag agctggaaaa attaaatact
    10861 ctctcccctt gcgggagaga gaccgaaaaa tacttcgttc agaagtattt ttcagagaga
    10921 gggtatatat ttaaaggatt tcaaatgagt attaaaaacg aaaaaattgc cgtcttattc
    10981 ggtggcgttt cgcaagagcg tgaagtttca ctgaattctg gtgcagcggt aacagctgca
    11041 ttaaaaagtt taggctataa cgtagaaggc attgatacta aagaattccc gattgaaaaa
    11101 ttaaaagaaa aagggattca gcgtgtattt aatattttac acggcggtat tggcgaaaat
    11161 ggcgtactgc aaggtgcgtt agagcaaatg ggtattcctt acaccggttg tggcgtaatg
    11221 gcatcagcag tgacgctgga taaattccgg actaaattat tatggaatgc agtaggttta
    11281 ccaacagcgg atatggtggt ggttcagcgt ggacaagcgg tcgattttga ccaaattatt
    11341 gcaaaattag gcttaccggt atttgtgaag tcttcgtgtg aaggctcaag tgtgggtgta
    11401 ttcaaagtaa aaaccaaaga agaattggca ccggcaattc acgaggcatt aaagtttgat
    11461 tccattgtgt tggtggaaga atttttagcc ggtaatgaat attctgtacc ggtattagac
    11521 ggacaagttc taccggcggt acaagtaatt ccggacggcg aattttatga ctaccacgca
    11581 aaatatgtgt cggataacac ccaatatgta gtaccggcat taaatgacga acgccaagca
    11641 gaggtcgcca aattagtgaa agcggcttac gatgtggttg gctgtcgagg ttggagccgt
    11701 attgatgtga tggaagatgg cgaaggaaaa ttccgcttgg ttgaagtaaa taccaacccg
    11761 ggaatgacca gtcacagtat tttcccaaaa tcagcagcaa cagttggtat ttcctttgaa
    11821 aaattagttg aacgcgtatt ggagttaagt gcttaatggg catcttccgc aagaagaaca
    11881 gcacaaatgt gcgtttgaat gttcttaatc caaaaacacc tgtttctcca cgtaaatggc
    11941 tagtattcat taagccatta attgtgttat tatgtacact atttgtattc ggtgtgtata
    12001 gtaattggca aaatatactc gaaagttggg ataaaacacc gattcgcgct tatgcattaa
    12061 cacataaaac acaatttaca acaaatgcag atattcgtga aactttgtct aaagaacctg
    12121 ctctaaaagg atatttcgga caaaatattc aagaggtcaa agataagctt ttatctatac
    12181 cttgggttaa agatgtggct gttcgtaaac tttatcccga ccgattaagt attactttgc
    12241 tagaacataa acccgttgca atttggaatg atactaactt tgtctcagaa caaggaaccg
    12301 tctttagttt accgaaagac agaatcgata aaaatggact tccattactc tatggtccag
    12361 atacggaagg aaaaagtgta ttagaggcat ggaataaaat ccgagctgat ctaaatgccc
    12421 gcaacttgga tttaacctcc gtttctgtag ataatcgagg ttcttggaca attaggcttt
    12481 ctaatcaagt agaattaaga ttaggaagag gggaatggac ttctaaaatt gatcgttttg
    12541 taacaatctt tcctgaaatt catatacctg aaggtaatag attagcttat gttgatttac
    12601 gttatccaca tggcgcggcg gttggattta gtcctataaa aaattaaact cagtgaacac
    12661 aaaaacatta tgacaaaagc agtagaatca aaaattatcg ttggtcttga cattgggaca
    12721 tcaaaggttg ttgccgttgt tggtgaagta ttacctgatg gtgtaattaa tgttatggga
    12781 gctggcgttt gcccttctaa aggagtcgat cgaggtggcg ttattgatct tgacgctgcc
    12841 gttaattcta ttcaaagagc cattgagcag gcggaatcta ttgccgattg tcaaattatg
    12901 ggtgtaacac tggcaatctc aggtcagcat attgttggtt taaatgaagc aggatcatca
    12961 acattatctg gaggtattgt tacccaagat aatattgaaa gtgctgtgga tatggcaaag
    13021 gcaattaaat tacaagatgg cttagaaaca ttacacatga ttccacaaga atatcacgtg
    13081 gatagattgc ctgcgactaa aaatccaatt ggcttatcgg gtatgcgttt acaagttcaa
    13141 gcacacttaa ttgcttgcca tcaagcttgg ttaaaaaact taaaaaatgc ggttgaacat
    13201 gctaaattaa aagtcgatca agttgtattt tcaggtcttg cctctagcta ttctgtttta
    13261 actgaagatg aaaaagagct gggtgtttgc ttaattgata ttggtggcgg tacgatggat
    13321 gtactagttt ataccgatgg agcattaaga tacagtaaag tcattccatt tgcaggtaat
    13381 aacattactg attatttggc tcgtgtattt acgacatctc gccctgaagc tgaaagcctt
    13441 aaagtaggtt atggtagcgc aatttcgcct ccaacccata attcagataa aaaaattgag
    13501 gtggcaggac taggtggacg gatggcaaga acctttaccc gagctcaggt ggcaacggtt
    13561 acctcacaat gttataacga tttgctaaaa gttgttgaag aggaattaac ccaattacgc
    13621 catgaacttt tccaaaaagg aataaaacaa gagttaattg ccggtgtagt cattactggt
    13681 ggtggctcac aaattgaaga tattgtagaa tgtgctaaat caatttttgg ttcacaagtt
    13741 cgtgttggat atccactcaa tattactggc ttaaccgatt atgtgaataa accacaatat
    13801 gcaacagcat tagggctttt acaatatagc cactataatt atactgaaca aggtacaagt
    13861 acagattcta atgaagatat ctttgctcct attggtcgtg gaaccaagaa aatcattgac
    13921 ttcttcaaat caaaatttta atagtgttaa aataaacacg cagggagata aaaatgtttg
    13981 aacctattat tgaacaaacg caagatgcta tcattaaggt agtgggtgtt ggtggcggtg
    14041 gcggtaatgc cgtagacaga atgtctcgct ctgccgatga tattaaaggt gttgaattct
    14101 ttgatgtaaa tactgatgct caagttttaa gaaaaagaac cacacgccaa actatccaaa
    14161 ttggagcaag tacaactaaa ggtttaggtg caggtgctga tccaatggta ggaaaacaag
    14221 ctgccgaaga agatcgtgag gctatcgcta atgcattaaa aggtgcaaat atgaccttta
    14281 ttgctgttgg tatgggtgga ggtaccggta ccggagcagc ccctgttgtt gctcaaattg
    14341 ccaaagagca aggctcacta actgtaggcg ttgtaacaaa acctttccgc ttcgaagggc
    14401 ctcgccgtat gcgctttgca gaccaaggca ttaaagagct ttctcaatat gttgattctc
    14461 ttattataat tcctaatgat aaattacgtg gtttaggtaa gcaaacaaca gcagtagatg
    14521 cctttgccgc agcaaatgat gttttaagta actgtgtgct tggtattacc aatatgatta
    14581 ccagttcggg cggttctacc ggtgcagata ttaacgtgga cttcgcggat gttcgtacag
    14641 taatgtctgg taagggacac gctatgattg gtacaggttt tgctgaaggt gaagttggtg
    14701 aaggacgagc ggaaaaagca atgaatgatg ccatttctag tccattactt gaaaatgtgg
    14761 atatttcagg tgcaagtggt atgctcatca acattagtgc tgggacagat ttcttattag
    14821 aagaagttta tgctatgatg gacttaattt atggctttgc aacagaagat gccgcaattg
    14881 tatttggttg taactattac cctgaaatgg atggaaaagt gagtgtcact cttgtcgcaa
    14941 caggaatagg ccaacctgag gaagctctac atatgccaca taaggctcag ccagtatatg
    15001 cacagcaagg aaatcatgtt caagctacac agcctactca gccaaaccat gcggtacaac
    15061 aaccaaactt tactcaaccg tcaagttatg gtcagccaaa tcaatttagt ggtcaaccac
    15121 aaccactaca acaacctgtg gctccaaaac cacaatcggt tgatacagca caaattttta
    15181 acccaaattc aattccgggt tttatgagaa atagtcaata acttccactc atttcggaag
    15241 taaggacaaa atatgactaa acaaagaaca ttaaaacaag cagcaaaagt aacaggtatt
    15301 ggcttacata gcggcaaaaa agtaacctta acgttacgcc ctgccccagc taatacagga
    15361 gttatttatg cccgtaccga tttagagcct gtcgtttatt tccctgcaag cgcagactct
    15421 attcgtgata cacaattatg cacatgtatg gtaaatgatg atggtgttcg tatttcaaca
    15481 gtagaacatt taaacgcagc aatgtcagca ttaggcttag ataatgttat tgttgaagta
    15541 gatgcacctg aaattccgat tatggacggt agttcaagcc catttatcta tcttttatta
    15601 gatgccggca tcgaagaaca agatgcaccg aagaaattta ttcgcattaa agaaacagta
    15661 cgcgttgaag aaggcgataa atgggccgaa ttaaaaccat acaataaagg ttttaaattg
    15721 aacttcacta ttgactttga gcacccatta atccccaaaa atgtagggag ctattccatt
    15781 gatttttctg ctgagaactt tattcagcaa ctaagtcgag cgagaacctt tacatttatg
    15841 aaggatgtag aatacttgca atctattggt cttgcattag gtggaagcct tgataatgct
    15901 attgtattag atgattaccg aattctaaat gaagacggtt tacgatttaa agatgaactg
    15961 gttcgccata aaatgctgga ttcgataggt gacttatata tgtgtggtta taatatccta
    16021 ggtgaattta gtgcatataa a
//
LOCUS       DANLXF010000040        15928 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.040, whole genome shotgun sequence.
ACCESSION   DANLXF010000040 DANLXF010000000
VERSION     DANLXF010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 15928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15928
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.040"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(53..1093)
                     /locus_tag="PXD66_000764"
     CDS             complement(53..1093)
                     /locus_tag="PXD66_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL6319088.1"
                     /translation="MKKQLRKNLTQNDRLQPQAQTEIFSFGDPIPVLDRADILNYLEC
                     SAMYEKWYNPPMSFDGLAKSLRSSTHHESAIITKANILLSTCEVDSRYLSRRDLSSFV
                     KDYLVFGNAYFEVVRNRLGQVQRIESPLAKYVRKGLEAGQFYYVPQRFDHQEHEFAKG
                     SIYHLLEPDINQDIYGLPQYLSALQSAWLNESATLFRRKYFLNGAHAGFVFYMSDASQ
                     KQEDVENIRQQLKQSKGVGNFKNLFVHAPNGKKDGIQIIPIADVSAKDEFFNIKNVSR
                     DDVLAAHRVPPQLMGIIPNNTGGFGNVADAAEVFFITEIEPLQERLKEFNQWLGQEVI
                     KFKPSKLLQRTQ"
     gene            complement(1102..2904)
                     /locus_tag="PXD66_000765"
     CDS             complement(1102..2904)
                     /locus_tag="PXD66_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HDL6319089.1"
                     /translation="MENENTITEYEVLAANPLHDKREAQSKYWAGYTVTEISRQLNIP
                     VSTIASWKKREKWDEISPVGRVEATLESRLNLLIMKESKNNNDYKEMDALRRLLESTA
                     RIKKYSNGGGNEADLNPNIKNRNKGDRKKPEQNAISEEQAELLINGFLDGMFHYQKKW
                     HEAGLTHRIRNILKSRQIGATYYFAHEALVDALVTGRNQIFISASKKQALQFRAYIVA
                     YAKRVADVELKGETITLPNESQLIFLGTNSKTAQSYHGNLYFDEIFWVNRFEEIRKVA
                     AGMASQKQYRITYFSTPSSITHSAYLLWSGKLFNRKRPKAEQVEIDISHANLKNGKKC
                     GDGQWRQIVNIYDAEAGGCNLFDIEQLKLENSPDEFEQLFMCEFIDDNQSVFKFTMMQ
                     RCLVDSMEVWRDYVFTDGYQRPFGNKEVWVGYDPSYTGDRSALVVIAPPKVDGGKFRL
                     LEYRTFKGADFAGQAAEIVAICAKYNVTRLAIDTTGLGVGVYEIVKKERPDAVALTYN
                     VELKSKMVLKGLDIISKGRFEFDSMHAVEVGASFMAIKKQITNSGRQVTYVADRSEEA
                     SHADLAWACLQVFINEPFDGKLEETTGTVDFLDD"
     gene            3054..3881
                     /locus_tag="PXD66_000766"
     CDS             3054..3881
                     /locus_tag="PXD66_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HDL6319090.1"
                     /translation="MTTKSKWFVVATEGATTDGREISREWLTQIEENYDPKNNYGARI
                     NLEHFRWRWFEKDDPHSYSYGDVLAVKTEEREDGKLQLLAEIAPTEALVKLVKDKQKV
                     YTSVEIDLNFADTGEAYLVGLAVTDTPASLGTEYLTFCAGATHNPLADRKQKPENLVS
                     EAVEANLEFTAEPEKGAWLEKFKAMFTKAKTDNDGKFAEHEKAMELLAEQFSKVSTEN
                     DRLQTELSTLQIEFTELQKQAGEFAEKFAKLEQEPSANYTPRPKATGAKAGDVQTDF"
     gene            3895..4923
                     /locus_tag="PXD66_000767"
     CDS             3895..4923
                     /locus_tag="PXD66_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HDL6319091.1"
                     /translation="MKDLTLEKYNAYLARQAELNNLPFNALATGIKFTVQPSVQQKLY
                     EKVRESSDFLKSISFVFVDEQTGETLGLDSAHTVASTTDTSGDGERKTTSIAKLVKQT
                     YHCQQINFDTHINYKQLDMWAKFPDFQQKVANVAAKQRKRDLIMIGFNGTSRAATSDR
                     NSNPLLQDVAKGWLQKMREDAKERVMNGESTDNQVLVGKGQEYANLDALVMDATEELI
                     DEWHRDDTDLVVITGRKLLADKYFPIVNQQNAPTEELAADIVISQKRIGGLKAVRVPF
                     FPANAILITKLENLAIYVQEGTTRKHIENVPKKDRIETYESENIDYVVEDYGCAALIE
                     NITLKDKE"
     gene            4933..5622
                     /locus_tag="PXD66_000768"
     CDS             4933..5622
                     /locus_tag="PXD66_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HDL6319092.1"
                     /translation="MRMSPARAHFLRKTAESESATPNRENLEGLQGYDLVLAQLKAHQ
                     RQLKKIQSIERKIEFKRNNFEQYRPWVEGSLAKGSGVQDTVITTMLIWSIDIGDYPTA
                     LAIAQYVLMHDLAMPEQFERTPATALVEELAEAAKKARDQKQPFEAAVLKQANDLTAE
                     FDMPDPVRAKLLRELGELIQEAEPQTALEHYQRAIALDPQCGAKGLRDKLEKQLAKQA
                     EEPKPAEEQNE"
     gene            5734..6249
                     /locus_tag="PXD66_000769"
     CDS             5734..6249
                     /locus_tag="PXD66_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HDL6319093.1"
                     /translation="MEQRNHVISIPKVNGYESSKNAIKPTAESDETITNNGFFPNIEL
                     LEVRNAMRIDGTVINERLKQAVIEAMATVNADLKAYRLNAEQAQKANLQACDDEQING
                     ESVLVYKYKRAVYCLAVANLYERYRSYDSTKDGHDKAEELESTAGDLKRDYHFAVRDI
                     LGENRMISELI"
     gene            6246..6458
                     /locus_tag="PXD66_000770"
     CDS             6246..6458
                     /locus_tag="PXD66_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HDL6319094.1"
                     /translation="MNTLIARQDDTLDELIFRHYGQTAGLVEQALEYNPELAHLPRLP
                     IGTVVTMPDIESRFATVAKSSVQLWD"
     gene            6464..6670
                     /locus_tag="PXD66_000771"
     CDS             6464..6670
                     /locus_tag="PXD66_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HDL6319095.1"
                     /translation="MHKTEQASYFGAIFGFFGSLTLSDWGVVVSILVAILTLLLNWAY
                     RAKEYKLKEREFELKLQNQGKKNE"
     gene            6663..7229
                     /locus_tag="PXD66_000772"
     CDS             6663..7229
                     /locus_tag="PXD66_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HDL6319096.1"
                     /translation="MSKSVKVGVFVCSVAAIIGTVKQYYSSEIRTSETGLAIIGNAEG
                     CRRDPYKCPADVITVGIGSTEASGEKINVNHKYTDKEIAERWIKDLKIAERCINRYFN
                     GDKMNENQFSAMVSAAFNMGCYNLRFYPNENGKYIQTTIHKYAMAKDFKAMCNRIPDF
                     NRSGGKVLRGLKIRREKEKALCLGQEVK"
     gene            7226..7681
                     /locus_tag="PXD66_000773"
     CDS             7226..7681
                     /locus_tag="PXD66_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319097.1"
                     /translation="MIVKLCQMTNKHFSPLMIVLVLVGVLANFVQFRQNESLKADLQA
                     RTTQNQLIQADNRNLANQLENSQIQLEAYQKQVDDLNQKVLAKMKQAEQRSNEILNEL
                     EKHKKWADSAVPPSVGKLLNQRKRAVSHPQIEPDNLSEKAGVPNTGNRD"
     gene            7830..8051
                     /locus_tag="PXD66_000774"
     CDS             7830..8051
                     /locus_tag="PXD66_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HDL6319098.1"
                     /translation="MSDQIDRANELAEKAREAALAKILQNQTACTSLFECEDCGEPIP
                     EKRREMVIGCTRCIECQTIYEHKQKGYRR"
     gene            8048..8533
                     /locus_tag="PXD66_000775"
     CDS             8048..8533
                     /locus_tag="PXD66_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6319099.1"
                     /translation="MIKPDRLRAVLTQTIDIFQANPEKLILQYDKGKIKSKGSQSHSF
                     EYHYDLELIVVDFPYHPDVLFVPVLNFVRNEQWELLQNPELQDKIEFEIDHNNHESYD
                     IYIRLPLTERVIVKEQDGHLTATHADEPNLADVSPFTRLTEYEVYLKDELIYQWAEPN
                     E"
     gene            8526..8984
                     /locus_tag="PXD66_000776"
     CDS             8526..8984
                     /locus_tag="PXD66_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HDL6319100.1"
                     /translation="MSEPIEQVKTAFDSLLNNISKPRRRLMYQQIGRELARSQRRRIK
                     AQQNPDGSAYEPRKKPKKGVKSKIKSGKMFDKITQPRFMRLRLESEGVSLGYEGGDAV
                     IARIHQQGLIGRVRKDWDLKVKYASRELLGFTDDDLQMIEDYMINILAGS"
     gene            complement(9035..9313)
                     /locus_tag="PXD66_000777"
     CDS             complement(9035..9313)
                     /locus_tag="PXD66_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319101.1"
                     /translation="MEILFILLGLIGFGALLAIGFTTFAAIFGVVTTVFLSLLSLLAN
                     MVGLSIIVILISLYVLLPLNTFILIALIFSAILGFLCWYHSDRQSVNQ"
     gene            9352..12264
                     /locus_tag="PXD66_000778"
     CDS             9352..12264
                     /locus_tag="PXD66_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6319102.1"
                     /translation="MSNNLKIQVVLSALDKLTAPFKSVVKQTEKLSQSLKVQRDELKA
                     LEKTQSKMQSFKKMDDSIKKSNETILKQTKHLDNLKNKISQMKNERIELKVKMDAKKR
                     EFQQITKGGSAWSARAAFLDKDFEKMQREYEKLGASISSVSRKRTEENRALKDSRHQQ
                     AKQLLYFRSLRRELRASGINIKAFAQSETNLTDKIAKANQLIDQQKAKLEKLNAVKAR
                     NEKYWATVEKVKNTSERLHNLGQRSMISGAAISAPVVGLGKGVVGMAQTAGKFEQFNA
                     ILEVTEGSAEKAKQSFDWVKQFAVDTPSNLDEAMEAFVKLRAYGLDPTNGLLQTLGDT
                     GAAMGKPVMQAVEAIADALTGENERLKEFGIKGSVVKGTNIIEYAYTDKLGKQQVAKV
                     NKNNRKEIEQTLTRIFNEKYAGAMEKQAKTLVGIWAKIEDYWTNFQMQVMESGAFDWI
                     KNKLQWVLDSLDKMAENGELQKWAEDVGAVIQETVQGLWAFGEKIIEVVKWLASLARE
                     NKGLIATFVKWSAILGSSLTVLGAFAVVASFALYPVARLALGIGKFTGASTLLNKALF
                     DSNDKFRLFNKSLYSSGTTAAFVKSKFNQLATLPNVLGSSFNKLGGGIKMLIPQLKTK
                     VFWLNMLKGILGKLWSAIRFIFSPLKLLLGIFSPIGLAITAISVAGVALYRNWEKVRA
                     FFGGFLQGLSKGLAPVLERFKPLGKLFETISNWVKSTFKWLSDLLTPADKTSESLDKA
                     SSAGEKFGLALAGAIDLVTSPVQWLVDQFKWISENMPSWDTIGEGVKKAATAIVPTEH
                     AKQITKTAEMSNNMYDPNYTPPQLEQKWIGGLVGNGKGRGFATGGYTGNGGKYEPAGI
                     VHKGEYVMTKEATARLGVANLNRLNYGKVAGLTALASSVAFAQPMPAVKIDSRPPLTA
                     SQPSPTVAPVSQNIHITINATGGQDPQAIARLVAMELEKQQRQAQARARSSLRDRG"
     gene            12326..12505
                     /locus_tag="PXD66_000779"
     CDS             12326..12505
                     /locus_tag="PXD66_000779"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319103.1"
                     /translation="MMFKEKGYDEFVNECVRKGREEHKAGLGFTLEQSRLHIKKTIEQ
                     KSQELNEFQREVVYG"
     gene            12498..12785
                     /locus_tag="PXD66_000780"
     CDS             12498..12785
                     /locus_tag="PXD66_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL6319104.1"
                     /translation="MAKITIKPQALSQFDEIVKSVIEFTGFETSGIRLQSDIFDKIEQ
                     IAFMPLSGRAREDGTRETFVRRYRIVYDYDRQADEVIILAIIHSSRLYPRP"
     gene            12914..13519
                     /locus_tag="PXD66_000781"
     CDS             12914..13519
                     /locus_tag="PXD66_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HDL6319105.1"
                     /translation="MSEPNHAEQIRRLDNIIRLGTIAEVDLTTATARVNSGGITTDFL
                     PWLTFRAGTSQTWSAPTVGEQCVVLSVSGEFTTGVILFGIYTQNAPSQSADEHLFRFS
                     DGAEISYNTASGQLTVKNCKVVIVQATESITLDTPTVKATKNVVIGGNLSVKGTTSSQ
                     GAISTQGAVTAKGDVSGAGISLGSHHHIEQGDGRPTSGAKP"
     gene            13519..13854
                     /locus_tag="PXD66_000782"
     CDS             13519..13854
                     /locus_tag="PXD66_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HDL6319106.1"
                     /translation="MNRETGEQLEDEVEHIKQSIKDILLTAKGSRVMRRTYGSNLYKL
                     TDKPIAASLIMQLSAACVMALQQWEPRIAITRFKVEFAEDNYNKLICTLDFTLKHRKI
                     SIKGMRFTL"
     gene            13851..14768
                     /locus_tag="PXD66_000783"
     CDS             13851..14768
                     /locus_tag="PXD66_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HDL6319107.1"
                     /translation="MSEIVDLSKLPAPKVLEELDYETLLAERKAKFLSLYPESERAVM
                     AARLALESEPITKLLQENCYLQLLERQRINSAAQATMLAYATGTDLDVIAANFNVERL
                     VVQAEDLTASPPIVEELEPDDDFRQRVQLKIESVSVAGPRKAYTFHALSANGKIADVS
                     VISPQPAYVTVTLLSRDGNGAVASEIINAAKIALNDEEVRPIADRVTVQGASIVEYEI
                     NAVLHLYRGPEKEPILKAAKANLEAYINQTKRIGRDVTHSGIHAALHVAGVQNVEILQ
                     PRTDLVLTPYQAGYCVNYTLNVEVSDEYS"
     gene            14755..15387
                     /locus_tag="PXD66_000784"
     CDS             14755..15387
                     /locus_tag="PXD66_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HDL6319108.1"
                     /translation="MNTVKALLPPGSSLLEKRAAECLQQAVQNPIKFANLINPHKCPV
                     KFLPYLAWALSVDYWEEHWSEELKRETIIRSFEEHRKKGTASAVRRIVESLGYAFEIR
                     EWFAEKRQRQPGTFRVFVELKDKGISEKIYQELERLIEDAKPVSRHITELAVSATSKG
                     KANIFSGAHSGEIITIYPPKKLPVIVIKSKMDLFSATNSGEIITIYPNKR"
     gene            15390..>15928
                     /locus_tag="PXD66_000785"
     CDS             15390..>15928
                     /locus_tag="PXD66_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6319109.1"
                     /translation="MEQKYKTILTHHGERVIVEALANKIPVPLKEMAIGDGNGSSITP
                     SASQTTLVREVYRAEITDLLEDPQNRHQMIAELLIPENVGGFIVREIGLFDEQGGLVA
                     VANCPENYKPVLEQGSGKVQYYRMILQVSSSDAVTLSINNNIVYATRTEFNEFVNNLS
                     SPDGYKHIGRCKSVAELRT"
BASE COUNT         5070 a         3339 c         3548 g         3971 t
ORIGIN      
        1 attaaaaaag ccccaatcta ggagctgtat tgtcagtttg taaagatctg ttttattgtg
       61 tccgctgtaa caatttagac ggtttaaatt tgataacctc ttgacctaac cactgattaa
      121 actctttcaa tcgctcttgc aatggctcaa tctcggtgat aaaaaacacc tcggcagcat
      181 cagccacgtt gccaaaaccg ccggtgttat ttgggataat ccccatcagt tgtggtggca
      241 cacggtgagc agctaacaca tcatcacggc tcacgttctt gatattaaaa aactcatctt
      301 ttgccgacac atcagcaatc ggaataatct gtattccatc tttcttgccg tttggtgcgt
      361 ggacgaacaa gtttttaaaa ttccccaccc cttttgactg cttgagctgc tggcgaatat
      421 tttcaacatc ttcttgtttt tgtgacgcat cgctcatata aaacacaaag ccggcgtgag
      481 ctccatttaa aaaatactta cggcgaaaca gagtagcact ctcatttaac cacgcagatt
      541 gcagggccga aagatattgt ggcaagccgt aaatatcttg attaatatca ggctcaagca
      601 agtgatagat tgagcctttg gcaaattcgt gttcttggtg atcgaatcgt tgtggcacat
      661 agtaaaattg accagcctct aaaccctttc gcacatattt tgccaaaggt gattcgattc
      721 gctgtacctg cccaaggcga tttcgcacca cctcaaaata ggcgttgcca aatactaaat
      781 aatctttaac aaacgatgac aaatcacggc gtgataaata acggctatca acctcgcaag
      841 tggaaagcaa aatatttgct ttggtgatga tcgcactttc gtggtgtgtt gatgacctta
      901 acgatttcgc caagccatca aagctcatcg gtgggttata ccatttctca tacatcgccg
      961 aacactccaa ataattgaga atatcggcac ggtctaacac cggaatcggg tcaccaaaac
     1021 tgaaaatttc ggtttgtgct tgcggttgta agcggtcatt ttgggttaaa tttttgcgca
     1081 attgtttttt cattttttgt cctaatcatc taaaaaatct actgtgccgg tcgtttcttc
     1141 cagtttccca tcaaacggct cattaataaa tacctgcaag caagcccacg caaggtcggc
     1201 gtggcttgcc tcttccgaac ggtctgccac ataagtcact tgccgtccgc tgttggtgat
     1261 ctgtttttta attgccataa aactcgcacc aacctcaaca gcgtgcattg agtcaaattc
     1321 aaatcgtcct ttgctgataa tatccaagcc ttttagtacc atcttggatt tcagctctac
     1381 gttgtaagta agagccaccg catctggtcg ttcttttttg acgatttcat aaacaccgac
     1441 accaagccct gtggtatcaa tcgccagtct ggtcacgtta tattttgcac aaatagcgac
     1501 aatttctgcc gcttgtccgg caaaatcagc ccctttaaaa gtgcgatact ctaatagtcg
     1561 gaatttcccg ccgtccactt tcggcggagc gattaccacc aacgccgagc ggtcgccggt
     1621 gtaagacggg tcatacccaa cccatacctc tttattgccg aatgggcgtt ggtagccgtc
     1681 cgtgaataca taatctcgcc acacctccat cgagtccaca aggcaacgtt gcatcatcgt
     1741 gaacttgaac acagactggt tatcatcaat aaactcacac ataaagagct gctcgaactc
     1801 atcgggcgag ttttccagtt tgagttgctc aatatcaaac aggttacagc cacctgcctc
     1861 ggcatcgtag atattcacga tttgtcgcca ttggccgtct ccgcattttt tgccgttttt
     1921 caagttggcg tgagaaatat caatttccac ctgttccgct ttcggacgtt tgcggttaaa
     1981 cagcttgccc gaccaaagca gataggcgga atgggtaatg ctcgaagggg tagaaaaata
     2041 agtaatccga tattgttttt ggctcgccat acccgctgcc actttgcgga tttcctcaaa
     2101 gcggtttacc caaaagattt catcaaaata gagattgccg tggtaagact gtgccgtttt
     2161 cgagtttgta cccaagaaaa ttagttgaga ctcgttcggc aaggtaatgg tttcgccttt
     2221 tagctccaca tcggcaaccc gtttggcgta tgccacaata taggctctaa attgcaatgc
     2281 ctgttttttc gaggcggaaa taaaaatctg atttctaccg gttactaacg catcaactaa
     2341 cgcctcgtga gcaaaataat aagtcgctcc aatttgacga cttttaagaa tattgcggat
     2401 tcgatgagtc agcccagctt cgtgccattt tttctgataa tggaacattc cgtctaaaaa
     2461 gccgtttatc aacagttcgg cttgctcttc ggaaatggca ttttgctcgg gctttttgcg
     2521 gtcgccttta ttacggtttt taatatttgg gttgaggtcg gcttcattac ctccgccgtt
     2581 agaatatttt ttgattcgtg ctgtactttc caacaaccga cgtaaagcat ccatttcttt
     2641 gtagtcgtta ttgtttttgc tctctttcat aatcagcaaa ttcagccgtg attccagtgt
     2701 ggcttccaca cgtccaacgg ggctgatttc gtcccatttc tctcgctttt tccaacttgc
     2761 aatggtagag acgggaatat ttagctgacg agaaatttcg gtcacggtgt aacctgccca
     2821 atactttgat tgagcctctc gtttatcgtg tagagggttg gcagccaaca cctcgtattc
     2881 agtgattgta ttttcatttt ccataggtgc gattatcaaa aatccacctt gtttaagcat
     2941 ttgcttgagg ctgtgaaacc cgatttaaca cgtaccacac attgaaaccg ccggcacaaa
     3001 acaagatcat aaatccgatt tttacccctc ccctttagag caaggaaaaa cagatgacaa
     3061 caaaatccaa atggtttgtg gtagcaacgg aaggagcaac tacagatgga cgcgaaatta
     3121 gtcgtgaatg gctcacgcag attgaagaaa attacgaccc gaaaaataac tatggtgctc
     3181 gtattaacct tgagcatttc cgctggcgtt ggtttgagaa agatgacccg cattcttaca
     3241 gctatggcga tgtgttagcg gttaaaacag aagaacgtga agacggcaaa ttacagttac
     3301 tggcagaaat cgccccaacc gaagccttag tgaagttagt caaagacaag cagaaagttt
     3361 atacctctgt ggaaattgac cttaattttg cggacacagg cgaggcgtat ttagttggtt
     3421 tggcggtgac tgatacaccg gcaagcctcg gcacggaata cctcacattt tgtgccggtg
     3481 caacccataa cccactagca gaccgcaagc aaaagccgga aaaccttgtg agtgaggcag
     3541 tagaggcgaa tttagaattt actgctgaac ctgaaaaagg tgcgtggctg gagaaattta
     3601 aggcgatgtt taccaaagcc aaaaccgaca atgacggcaa atttgccgaa cacgaaaagg
     3661 caatggaact gttagctgag caatttagca aagtttcgac agaaaacgac cgcttgcaaa
     3721 ctgaactaag cacattgcaa atcgaattta ccgaattgca aaaacaagcg ggcgaatttg
     3781 ccgaaaaatt tgcaaaactg gaacaagaac cgtctgccaa ttacacccca cgcccaaaag
     3841 ccactggtgc gaaagcaggt gatgtacaaa ccgattttta attgaggatc acgaatgaaa
     3901 gatttaaccc ttgaaaaata taacgcctac cttgcacgcc aagctgaatt aaacaacttg
     3961 ccgtttaatg cattggcaac cggcattaaa ttcacagtgc aaccatcggt acagcaaaag
     4021 ctgtatgaaa aggtgcgtga aagctctgat ttcttaaaat ctatcagctt tgtgtttgtt
     4081 gatgagcaaa ccggtgaaac cttaggttta gattcagctc acaccgtggc cagcaccacc
     4141 gataccagcg gtgacggtga acgcaaaacc acctctattg cgaagttggt aaaacaaacc
     4201 taccactgcc aacaaatcaa ttttgacacc catatcaatt ataaacagtt ggatatgtgg
     4261 gcgaaattcc ctgattttca gcaaaaagtg gcgaatgttg cggcaaaaca acgcaaacgt
     4321 gacttaatta tgatcggctt taacggcaca agtcgtgcgg caaccagtga tcgcaacagc
     4381 aacccgttat tgcaagatgt ggcaaaaggc tggttgcaga aaatgcgtga agatgccaaa
     4441 gagcgtgtaa tgaatggcga aagcacagac aaccaagttt tagtcggcaa aggtcaagag
     4501 tatgccaatc ttgacgcact cgtaatggac gccaccgaag aacttattga tgaatggcat
     4561 cgtgatgaca ccgatttagt ggtgattacc ggtcgcaagt tgcttgcgga taaatatttc
     4621 ccgattgtga atcagcaaaa cgcaccaacc gaagaacttg ccgctgatat tgtgatctca
     4681 caaaaacgca ttgggggctt aaaagcggtg cgtgtgccgt tcttcccggc gaatgcgatt
     4741 ttaatcacta agctggaaaa tttagccatc tatgtgcaag aggggacaac ccgcaagcat
     4801 attgagaacg tgccgaaaaa agaccgtatt gaaacctacg aatcagaaaa tatcgactac
     4861 gttgtcgaag attatggctg tgcggcatta atcgaaaaca ttacactcaa agataaagag
     4921 taaggtggaa ctatgcgaat gagtccggct cgggctcatt ttttgcggaa aaccgcagaa
     4981 agtgagtctg caaccccaaa ccgagaaaac cttgaaggct tacaaggcta tgacttagtt
     5041 ttagcccaac ttaaagccca tcaacgccaa ttaaagaaaa tccagtcgat tgagcgaaag
     5101 attgagttta aacgcaacaa ttttgagcaa taccgacctt gggtggaagg ctcccttgcc
     5161 aaaggctccg gtgtgcaaga cacggttatc accacaatgc tgatttggtc gattgatatt
     5221 ggcgattacc caaccgccct tgcgattgct caatacgttt taatgcacga tttggcaatg
     5281 ccggagcagt ttgaacgcac accggcaacc gccttggttg aagagcttgc agaggcggca
     5341 aagaaagccc gagaccaaaa acagccgttt gaggcagccg tgctgaaaca ggcaaacgac
     5401 ctcaccgctg aatttgatat gcccgaccca gtgcgagcca aattactgcg tgagctaggc
     5461 gagctaatcc aagaggcaga gccacaaacc gcccttgaac actatcaaag agcgatagcc
     5521 ctcgaccctc aatgcggtgc aaaaggcttg cgagacaagt tagaaaagca acttgccaaa
     5581 caagccgaag agccgaaacc agcagaagaa cagaatgagt aggcgaaagc cgaaatagag
     5641 cgtatccacg ccagccaagg ggcggatttt aaagggtgat ttattcattt tgcacccttt
     5701 taaaatcccc accccttacc ttacggaaac cttatggaac aacgcaatca cgttattagt
     5761 atcccgaaag tgaatggcta tgaatccagc aaaaatgcga tcaagcccac cgcagaatct
     5821 gatgaaacta tcaccaataa tggctttttt ccgaacattg agctactgga ggtgcgaaat
     5881 gcaatgcgga ttgatggcac ggtcattaat gagcggctaa aacaagcggt gattgaggca
     5941 atggcaacag tcaatgccga cttaaaagcc tatcgcctca atgcagagca agcacaaaaa
     6001 gcgaacttgc aagcctgtga tgatgagcaa atcaacggcg aaagcgtgct ggtttacaaa
     6061 tataaacgag cggtctattg ccttgcagta gcgaaccttt acgaacgcta ccgcagttat
     6121 gacagcacca aagacggcca cgacaaagcc gaagagctag aaagtaccgc cggtgatttg
     6181 aaacgagatt accactttgc cgtgcgtgat attttgggcg aaaaccgaat gattagtgaa
     6241 ttgatatgaa caccttgatt gcacgccaag acgacacctt agacgagctg atctttcggc
     6301 actacgggca aacggcaggc ttagtcgagc aagccttaga atataacccc gaactggctc
     6361 atttgccacg tttgccgatc ggcactgtag ttacaatgcc ggatattgag agccgttttg
     6421 caacggtggc gaaatccagc gtgcaacttt gggattaaaa aatatgcaca aaactgaaca
     6481 agcctcctat tttggggcaa tttttggctt ttttggtagc ctcaccctat ctgattgggg
     6541 ggtggtagtg agtattctgg tagctatttt aaccttatta cttaattggg cataccgagc
     6601 caaagaatac aaactgaaag agcgagaatt tgagctaaag ctacaaaacc aagggaaaaa
     6661 gaatgagtaa aagtgtaaaa gttggcgtat ttgtctgctc cgttgcagct attatcggga
     6721 cggtaaagca atattacagc tccgaaatcc gcaccagcga aacaggctta gccattatcg
     6781 gcaatgcgga gggttgcaga cgtgatccgt ataaatgccc tgccgatgta atcacggttg
     6841 gcattggctc aaccgaggca agcggtgaga aaattaatgt caatcacaaa tacaccgata
     6901 aagagattgc cgagcgttgg ataaaagatc ttaaaatagc tgaaaggtgt atcaatcgtt
     6961 attttaatgg cgacaaaatg aacgaaaatc agttttcggc gatggtatca gcagcattta
     7021 atatgggatg ttacaacctt agattttatc caaatgaaaa tggtaaatac attcagacaa
     7081 caatccataa atatgctatg gctaaggatt ttaaagcgat gtgtaatagg atacctgatt
     7141 ttaaccgctc gggaggtaaa gtgctaagag gtttgaaaat ccgccgagaa aaagaaaaag
     7201 ccctgtgtct cgggcaggag gttaaatgat tgtcaaactc tgccaaatga ccaataagca
     7261 tttcagcccg ctaatgattg tgctggtgct agttggcgta ttagccaatt tcgtgcagtt
     7321 tcgccaaaac gaaagcctga aagcagactt acaggcacgc accacgcaaa accaactgat
     7381 tcaagccgat aaccgcaatt tagccaatca gcttgaaaac agccaaatac agcttgaggc
     7441 gtatcaaaaa caggtggacg acctcaatca gaaagtgtta gccaaaatga aacaagcgga
     7501 gcaacgaagt aatgaaatcc ttaacgaact ggaaaagcat aaaaagtggg ctgattctgc
     7561 cgtgcctcct agcgttggca agctgctcaa ccaacgaaag cgtgcggtat cacaccctca
     7621 aatcgaaccc gataatctgt ccgaaaaggc tggagtgcca aacaccggca accgagatta
     7681 acaccaacgg tgatcttgtc aaagcactag acaaaagact caacaccatt gaggtatgcc
     7741 aagtcatcat cgaatcgttc gagcattgca tcgagcaata caacaaaatc aaccaaggcg
     7801 agtgatcgcc ttttttaata aggacgacta tgtcagacca aatcgaccga gccaacgaat
     7861 tagccgaaaa agccagagag gcggcacttg caaaaattct gcaaaatcag accgcttgca
     7921 ccagcctttt tgagtgtgag gattgcggag agccaatccc cgaaaaacgc cgagaaatgg
     7981 ttattggttg cacccgttgc attgagtgcc aaaccattta cgagcataag caaaaaggct
     8041 accgcagatg attaaacctg atcgcttgcg agccgtgctc acacaaacca ttgatatatt
     8101 ccaagctaac cccgaaaagc tgattttgca atatgacaaa ggcaaaatta aaagcaaagg
     8161 gagccaaagc cattcgtttg aataccacta cgatttagag ctaatcgtgg tggattttcc
     8221 gtatcacccc gacgtgttgt ttgtgccagt gctaaatttt gtgcgaaatg aacaatggga
     8281 gctactgcaa aacccggaac tacaagacaa aatcgaattt gaaatcgacc acaacaatca
     8341 cgagagctac gatatttata tccgcctccc gcttaccgag cgggtgatcg tgaaagagca
     8401 agacggacac ctaactgcaa cccacgccga tgaaccgaac cttgccgatg tttcgccatt
     8461 tacccgttta accgaatatg aggtttacct caaagacgag ctgatttatc agtgggcaga
     8521 gccaaatgag tgaaccgatc gaacaagtaa aaaccgcctt tgatagctta ctgaataata
     8581 tcagcaaacc tcgcagacgg ttgatgtatc aacaaatcgg gcgagagctt gcccgaagcc
     8641 aacgcagacg gattaaagca caacaaaacc cagacggctc ggcatacgag ccacgcaaaa
     8701 agccgaagaa aggggtaaaa agcaaaatta aatcgggcaa gatgtttgac aaaattaccc
     8761 agccacgttt tatgcggcta cgccttgaaa gcgaaggtgt cagcttaggt tatgagggtg
     8821 gtgatgcggt catcgcccga atccaccaac aaggcttaat cggacgagtg cggaaagatt
     8881 gggatttaaa agtgaaatat gccagccgtg agttgctagg ttttactgat gacgatctgc
     8941 agatgattga ggattatatg attaatattt tggctggtag ctaaatgatg aatagaaact
     9001 tttgcaaaaa agcggtcaat tttgaccgct tgagttattg attaacgctt tgtctatctg
     9061 aatgatacca acaaagaaac cctaaaatag ctgaaaaaat caatgctatc aaaataaaag
     9121 tattgagtgg taacaataca taaagtgaga tcagaataac gataatagag agcccaacca
     9181 tatttgccaa taatgaaagc aggcttaaaa atactgttgt aactacacca aaaatggcag
     9241 caaaggttgt aaaacctatt gccaataatg caccgaaacc aattaaccca agtaagataa
     9301 ataagatttc catagatttt cccttagcca tactacaatt aaggttataa tatgagcaat
     9361 aatttaaaaa ttcaagtagt tctttctgcg ttagataaat taaccgcacc atttaagagt
     9421 gtggtaaaac agacggagaa attatctcaa tctttaaaag tacagagaga tgaattaaaa
     9481 gcattggaaa aaacacaaag taaaatgcaa tcatttaaaa aaatggacga tagcattaag
     9541 aaatccaatg aaactatact caaacagaca aagcatttag ataatctcaa aaataaaatc
     9601 agccaaatga aaaatgaacg aattgagctg aaagtgaaaa tggatgcgaa aaaaagggaa
     9661 tttcaacaaa ttacgaaagg tggttcagct tggagtgcaa gagccgcatt tttagataaa
     9721 gattttgaaa aaatgcaaag agaatatgaa aaactcggtg catctatttc aagtgtcagc
     9781 cgaaaaagaa cagaagaaaa tcgggcatta aaggattctc gccatcaaca agctaagcaa
     9841 cttttatatt ttcgtagttt aagacgtgaa ttgagagcaa gcggcattaa tattaaagct
     9901 tttgctcaaa gcgaaaccaa cttaacagac aagattgcaa aagcaaatca attaatcgac
     9961 caacaaaaag caaaattaga aaaactaaat gcggttaaag ctcgaaatga aaaatattgg
    10021 gcaactgtcg aaaaggtaaa aaataccagc gaacgtttgc ataatttagg acaacgctca
    10081 atgattagtg gtgcggcaat ttctgcccct gttgttgggc tagggaaagg ggttgtcgga
    10141 atggctcaaa cagcgggtaa atttgaacaa tttaatgcta ttttagaggt aaccgaggga
    10201 agtgctgaaa aagccaagca aagttttgat tgggttaaac aatttgcagt agataccccc
    10261 tcaaaccttg atgaagcaat ggaagccttt gtaaagttaa gagcatacgg tcttgatcct
    10321 accaatggct tactacaaac attaggtgat acaggtgctg caatgggtaa gcctgtaatg
    10381 caagcagttg aggcaatagc agatgcttta acgggtgaga atgaacgctt aaaagaattt
    10441 ggtattaaag gaagtgtagt taaaggtaca aatattattg agtatgccta cactgataaa
    10501 ttaggcaaac aacaagtcgc aaaagtaaac aaaaataacc gaaaagaaat tgagcaaacc
    10561 ctaacccgta tttttaacga aaaatatgct ggagcaatgg aaaaacaagc taaaacgcta
    10621 gttggtattt gggcaaaaat tgaagattat tggacaaatt tccaaatgca agtgatggaa
    10681 tcgggagcat ttgactggat taaaaataaa ttgcaatggg tgttagacag tttagataaa
    10741 atggctgaaa atggcgagtt gcaaaaatgg gctgaagatg tcggagccgt tattcaagaa
    10801 acagtacaag gcttatgggc ttttggtgaa aaaattattg aagtggttaa atggttagcg
    10861 agcttggcac gggagaataa agggctaatt gcaacattcg ttaaatggtc tgctattctt
    10921 ggctctagtt taacggtgct aggagctttt gcagtggtgg caagttttgc attatatcct
    10981 gtagcaagat tggctcttgg tatcggcaaa tttacaggtg caagcacctt attaaataag
    11041 gcattatttg atagtaatga taaattccga ttattcaata aatcacttta ttcatcaggc
    11101 actacggctg cttttgttaa atcaaaattt aatcagttag caactttacc aaatgtgctg
    11161 ggaagtagtt ttaataaatt aggcggtggc atcaaaatgc taattccaca gcttaaaaca
    11221 aaagtatttt ggctcaatat gctaaaaggt attttaggca agttgtggag tgcaatccgt
    11281 tttattttct caccgttgaa attattactt ggtattttct cgccgattgg cttagcgatt
    11341 acagctattt cggtagcagg ggtagcgtta tatagaaatt gggagaaggt cagagccttt
    11401 tttggaggct ttttgcaagg attatctaaa ggtcttgcac cagtattaga gagatttaaa
    11461 cctttaggaa aactatttga aactatatca aactgggtta aatctacgtt taagtggtta
    11521 tccgatttat taaccccagc ggataaaaca tcggaaagtt tagataaagc gagttctgca
    11581 ggagaaaaat tcgggcttgc attggctggt gcaatagact tggtaacatc acctgttcag
    11641 tggctagtcg atcagtttaa gtggatttct gaaaatatgc cgagttggga cactattggt
    11701 gagggtgtaa aaaaggcagc tactgcaatt gttcccaccg agcacgccaa gcagattact
    11761 aaaacggcag aaatgtcaaa caatatgtat gaccccaatt atactccacc acaacttgag
    11821 caaaaatgga ttggtggctt ggttggtaac ggtaaaggaa gaggttttgc cacaggcggc
    11881 tacaccggca atggtggcaa atatgagcct gccggtattg tgcataaggg cgagtatgta
    11941 atgaccaaag aggcaactgc acgcttgggt gtggcaaatc ttaaccggtt aaattatggc
    12001 aaagttgctg ggctaactgc acttgcgtca agtgttgcgt ttgctcaacc aatgcctgcg
    12061 gtcaaaatcg acagcagacc accgctgacg gcaagccaac caagcccaac agttgcaccg
    12121 gtgtcgcaaa atatccatat caccatcaac gccaccggtg ggcaagaccc acaagcaatc
    12181 gcccgactgg ttgcaatgga gttggaaaaa caacaacgcc aagcacaagc aagagcaaga
    12241 tcgtctttga gagacagggg ctaatttgac aaagtagaat gaaaagcgga caatgctcta
    12301 aattgtccgc tttttatagg agcaaatgat gtttaaagaa aaaggatatg atgaatttgt
    12361 gaatgaatgt gtgcgaaaag gtagagaaga acacaaagct ggtttaggat ttacgctaga
    12421 gcaatctcgc ttacatatca aaaaaaccat tgagcaaaaa tcacaagagc taaacgaatt
    12481 tcagcgtgaa gtggtttatg gctaaaatta ccattaaacc acaagcattg agtcaatttg
    12541 acgagattgt aaaaagtgtt atcgaattta ccggttttga aacgagtggt attcgattac
    12601 aaagtgatat ttttgacaaa atagagcaaa ttgcatttat gccactctct ggaagagcaa
    12661 gagaagatgg gacaagagaa actttcgtca gacgttatcg tattgtgtac gattacgata
    12721 ggcaggcaga tgaagtaatt atcttagcta tcattcacag tagccgactt tacccacgcc
    12781 cataatcagt aagcgaccta atcggtcgct ttttctttgc ctgttaaaac ccatttcaca
    12841 cctcgcattt atacccaaag cctttcgcct tttgcaaaat atcctcattt tttaaccgct
    12901 tgtaattgta aaaatgagtg agccaaacca cgccgaacaa atccgccgtt tagacaatat
    12961 tatccgactt ggcactattg ccgaggtgga tttaaccaca gcgactgcac gggtcaattc
    13021 gggcggtatt acgaccgatt tcttaccgtg gctgactttt cgtgccggta catcgcaaac
    13081 gtggtcagct ccaacagttg gcgagcaatg cgtggtgtta agcgtgagtg gtgaatttac
    13141 cacaggcgtg atcttatttg ggatttacac ccaaaacgcc ccgagccaat ccgccgatga
    13201 gcatctattc cgcttttcgg acggtgcaga aatcagctac aacaccgcaa gcggtcaatt
    13261 aaccgtaaaa aattgcaaag tggtgattgt gcaggcgaca gaatctatca cgctcgacac
    13321 gcccacggtt aaggccacaa aaaatgtcgt gattgggggc aacctatccg tgaaaggcac
    13381 aacctcatcg caaggtgcaa tcagcacaca aggggcggta acagcaaaag gcgatgtatc
    13441 aggggcggga atcagtttag gcagccacca tcacattgag caaggtgacg gcagaccaac
    13501 tagcggagca aaaccataat gaaccgagaa acaggcgaac agcttgaaga cgaagtagag
    13561 catatcaagc agtctatcaa agatattttg ctgacggcaa aaggcagtcg ggtaatgcgg
    13621 cgaacctatg gcagtaatct ctacaagcta actgataaac cgattgccgc aagtctgatt
    13681 atgcaactct ccgctgcgtg cgtgatggcg ttgcagcaat gggagccacg tatagcgatc
    13741 acccgattta aagtggaatt tgccgaagat aactacaata agctgatttg caccttagat
    13801 tttaccctta agcaccgaaa aatcagcatc aaaggaatgc gttttacact atgagcgaaa
    13861 ttgtcgattt atccaaactc cccgccccga aagtactgga agaattggac tatgaaacct
    13921 tactggcaga gcggaaagcc aagtttttat ccctctaccc agaaagcgaa cgagcggtaa
    13981 tggctgcacg gttagcgttg gagtctgagc ctatcaccaa gttactacaa gaaaattgct
    14041 atttgcagct actcgaacgc caacgcatta atagtgcggc tcaagccaca atgcttgctt
    14101 atgctaccgg tacggattta gatgtcatcg cagccaactt taatgttgag cggcttgtag
    14161 tgcaagctga agacttaact gcaagcccgc ctatcgtcga agagttagag cctgatgatg
    14221 atttccgtca gcgtgtgcag ttaaaaattg aaagtgtttc ggttgccggt ccacggaagg
    14281 cttacacctt tcacgccctg tctgctaacg gaaaaattgc cgatgtgtcc gtcatatcac
    14341 cacagccagc ttatgtaacg gtaacgctgc tttctcgtga tggcaacgga gccgtagcct
    14401 cagaaatcat caatgcggcc aaaattgctc tcaatgatga agaggttaga ccgattgccg
    14461 atagagtaac cgtgcaaggg gcaagcattg tggaatatga aattaatgcg gtgttgcatc
    14521 tctaccgtgg acctgagaaa gagcctattt taaaagcggc aaaagctaac ttagaggctt
    14581 acatcaacca aacaaaacgc attggacgag atgtgactca ctcaggtatc cacgccgcct
    14641 tgcacgttgc cggtgtgcaa aatgtggaaa ttttgcagcc gagaactgat ttggtgctga
    14701 caccttacca agccggttat tgtgtcaatt acacgctaaa tgttgaggtg agcgatgaat
    14761 acagttaaag cattattgcc acccggttca tcacttttgg aaaaacgagc tgccgaatgt
    14821 ttgcaacaag cggtacaaaa tccgattaaa tttgcaaatt tgattaatcc acacaaatgc
    14881 cccgtgaaat ttttgcccta tcttgcgtgg gccttatcgg tggattattg ggaagagcat
    14941 tggagtgaag aattaaaaag agaaaccatt attcgatctt ttgaagaaca ccgaaagaaa
    15001 ggcacagcaa gtgcagtgcg aagaattgtg gaatctctcg gctatgcttt tgaaatccgt
    15061 gagtggtttg cggaaaaacg gcaacggcag cccgggacgt ttagggtttt tgttgagtta
    15121 aaagataaag ggattagcga aaaaatctac caagaattag aaaggttgat tgaagatgca
    15181 aaaccggtat caaggcacat cacagaatta gcagtatcag ccacctcaaa aggcaaagca
    15241 aatattttta gtggagcaca cagtggcgaa attattacga tttatccacc taaaaagctc
    15301 cctgttattg tcattaaaag caaaatggat ttatttagtg caacaaacag tggcgaaatt
    15361 attacgattt atcctaataa gaggtaaaaa tggaacaaaa atataaaaca atattaaccc
    15421 atcacggtga gcgagtgatc gttgaggctc ttgcaaataa gataccagtg ccacttaagg
    15481 aaatggctat aggagatggt aacgggtcat caataactcc aagtgcatct caaaccacac
    15541 ttgtaagaga agtttatcgt gcggaaatta cagatttgct agaagacccg caaaatcgcc
    15601 atcagatgat tgctgaatta ctaatcccag aaaatgtagg tggatttatt gtccgagaaa
    15661 tagggttatt tgatgaacaa ggtggtttgg ttgctgtagc aaattgccct gaaaattata
    15721 agcctgtatt agaacaaggc agtggtaaag tccaatatta ccgaatgatt ttacaagtta
    15781 gtagtagtga tgcggtcacc ttatcgatta acaataatat tgtttacgcc acaagaactg
    15841 agtttaacga atttgtaaat aacttgagta gtcctgatgg ctataaacac atcggacgct
    15901 gcaaatctgt agcagaactc cgcactat
//
LOCUS       DANLXF010000041        15644 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.041, whole genome shotgun sequence.
ACCESSION   DANLXF010000041 DANLXF010000000
VERSION     DANLXF010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 15644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15644
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.041"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(199..1074)
                     /gene="ubiA"
                     /locus_tag="PXD66_000786"
     CDS             complement(199..1074)
                     /gene="ubiA"
                     /locus_tag="PXD66_000786"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HDL6319110.1"
                     /translation="MNNETLFSKQKWIAYAQLMRFDKPIGTLLLLHPTLWALFIAAEG
                     MPPVAILAIFTLGVIVMRAAGCVINDYADREIDGHVKRTSQRPLATGRVTTTEAKVIF
                     AILLLFAFLLDLMLNRYAFLLSFVAVFLAVIYPFMKRFTHLPQVVLGMAFGWSIPMAF
                     GAVTETLPLECWLLFFANLAWTVAYDTQYAMVDRDDDLRIGVKSTAILFAQYDNKIIA
                     LLQFITLILLGLLGVVKDYHIGYFVVLALSATLFIYQCWLTKKRERSECFKAFLNNHY
                     FGLGVFVAILVGIYF"
     gene            complement(1067..1828)
                     /locus_tag="PXD66_000787"
     CDS             complement(1067..1828)
                     /locus_tag="PXD66_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HDL6319111.1"
                     /translation="MKQANRHKKIIELVNQLGYVSTEQLVAELNVSPQTIRRDLNEMA
                     ENNLIRRHHGGAAAPSNTENSDYTHRKQFFSHEKNAIAQQVAKLIPNGASLFLDIGTT
                     SEAVALALLSHKNLKVVTNNLNAAHILMQNEDCQITVAGGGLRSDGGLIGEETVRFIN
                     QFRLDFGILGISAVDMDGSMLDYDYHEVQVKRALMECSRQVVLVTDHSKFTRTAIARL
                     GNVKEVNYLFTDQDLPLELQTHLSQSDVIVKICNE"
     gene            complement(1895..3262)
                     /gene="purB"
                     /locus_tag="PXD66_000788"
     CDS             complement(1895..3262)
                     /gene="purB"
                     /locus_tag="PXD66_000788"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDL6319112.1"
                     /translation="MELTALTALSPIDGRYQDKVASLRPIFSEFGLLKFRVTVEVRWL
                     QKLASHTQINEVPVFSEKANDYLNQIVADFSLEDANRIKTIERTTNHDVKAVEYFLKE
                     KCEALPELQAVNEFIHFACTSEDINNTSHALMLKTAREEVLLPEWKKVIDAVAELAKC
                     YQHIPLLSRTHGQPASPTTMGKEMANVAYRLQRQYKQLENLEILAKINGAVGNYNAHL
                     SAYPEIDWHTFSQEFVESLGVTWNPYTTQIEPHDYIAEFFDCVARFNTILIDFDRDMW
                     GYIALNHFKQRTIAGEIGSSTMPHKVNPIDFENSEGNLGLANAVMAHLGQKLPISRWQ
                     RDLTDSTVLRNLGVGLGYALIAYASTLKGISKLEVNEQHLRDELNQNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDETAMREFIDKLEIPENEKNRLKEMTPASYIGYAVE
                     LVDKL"
     gene            complement(3296..3925)
                     /gene="hflD"
                     /locus_tag="PXD66_000789"
     CDS             complement(3296..3925)
                     /gene="hflD"
                     /locus_tag="PXD66_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HDL6319113.1"
                     /translation="MADYHDITLAYAGVCQAATLVQQFAHKGMADREAFKCALESLLI
                     TQPESTLSVFGDDLSHLKMGLETALAQTGGGNGKLDTEVGRYWISLLALSQKLNKNPE
                     AKQQLAQRLQQVERQLALYEDNVMADQMIANLAAIYSDIISPLGTKIHVMGMQDYLVR
                     PDIQNKIRASLLAGIRAGILWQQVGGSRWQFLFSRKKIFNQAQKFYRQI"
     gene            complement(4057..5721)
                     /gene="cydC"
                     /locus_tag="PXD66_000790"
     CDS             complement(4057..5721)
                     /gene="cydC"
                     /locus_tag="PXD66_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HDL6319114.1"
                     /translation="MKSLFPFFALYQTHFGRLLLGVVLAILGLAASIGLLSLSGWFLA
                     ASFLAGNALIFNFFYPSSGVRGLAVGRTISRYFERLVTHDATFRVLANLRASVFKKLI
                     PLSPSGLNRFRNSELLNRLVADVDTLDTLYLNLVSPFVSAVMIIAFMAIGLSFVSVPL
                     MLIICGTLLVLLAVIPTVFYRLGLKLGRNAIQSRANYRSQFIEWVQLNAEFLLFGNLN
                     QMSEKLQQTEQQWLNAQSKESRLSGLSNSLIMLSNGILTCVVIYLVSTSINVPTAKYP
                     EALIALVIFCVMASAEILSPIGIAFLHLGQVITAAERISEITEQQPNVTFGSKAEWQN
                     LSQNQPLVRCENVSFAYYGEQNVLNNLSFKVLKGQKVAILGKTGSGKSTIFQLLNRNY
                     DPTSGQIWLNNCKIDEYSEPMLRSKLVTLSQRVHIFSQTLKDNLLMGNAQATDEQMQE
                     AVRKVGLGHLLETDGLNLWLGEGGRPLSGGEQRRLGLARLLLSSAELVLLDEPTEGLD
                     RETEQQILNLILTHCCDRTLIMITHRQSGLDKFDVVYRIDNGRLVG"
     gene            complement(5721..7487)
                     /gene="cydD"
                     /locus_tag="PXD66_000791"
     CDS             complement(5721..7487)
                     /gene="cydD"
                     /locus_tag="PXD66_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HDL6319115.1"
                     /translation="MEKQRQRELQKWLKSQQKVIKKYMHLNILLGSFSSLCMIGQMWL
                     IATMLDKMLIGKQSPNSFLVEIMLLFCCFAARAFFIFLRERVGFKAGQTLRLHLRRQI
                     LAKMEAVGPMSIQQKPAGSWATLMLEQVENLHNFYARYLPQQFLSLIVPLLILCFVFP
                     INWAAGAILFATMPLLPLFMILAGMKAVEANQRNIGILSRISGQFLDKLKGLETIRLF
                     GQAEKQTEQIYQSTEDFRISTMDVLKMAFLSSAVLEFFTAVSIAVTAVYFGFIFLGEL
                     DFGYYGTGVTLFIGFFCLMVAPEFYQPMRELGVFYHDKAAAISAADNIETFLKEKVKT
                     QGGNLQENVENQPLVIQAKDCVIFSPQGKALTQALNFELNAHQHIALVGQSGAGKSSL
                     MNMLLGFLPYEGSVTINGTELRDLNLSQWRAKLAWVGQNPQLMHGSLKENILIGNPNA
                     TDSELAKALQLSKADEFVARLGLEHQVQDSNIGISGGQAQRIAIARALLRPYELLLLD
                     EPTASLDMDSEQQVLAALHNLSREQTTLMITHRVEDLTQCDEIWVMKQGQIIQKGRFT
                     ELEHTGFFAELIHNELVHQGEI"
     gene            complement(7602..11699)
                     /locus_tag="PXD66_000792"
     CDS             complement(7602..11699)
                     /locus_tag="PXD66_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB"
                     /protein_id="HDL6319116.1"
                     /translation="MTDENKQTATNQTDSAQEPQATQPKKSKWRFLKHGFCLTFILLL
                     LAILFLCTGYGQRKVLSWVDKFVEPLQLGQVQGSLQDGLQLSDARFVMDGVNVTVGQA
                     DLHIGFSCLLHYEMCLNNLSLKDTEVLIDTTKLPPSQPKEETEPFTELNLPLGISAQN
                     INLENIKVKVDDMDIHLNHFHTALSGKGRAITVHPTQLEGLNLLLALKLDETDTEQAV
                     DFAQKNAKTEALSATNPQEIADKVIQEAVEKTSEKVVEGFHQLQRKTVAQAEQVDNKV
                     DWAAIKAQLAQPLLDKNIRFSLPLDLNIEQIDIANVAIAQKAKDEKGNLIEPISLLNV
                     ASLQLQAQAKDQHINLKSLDFKSDQGDLTASGSLSLQDNYPLSWTLSGHEAKTAKVKL
                     PFNQIEASLSGELYDKTSLKVKTAGAISASLEGNVALAQAKTPFDLSLKSESMRYPFA
                     PQQNGDVLSLENIAMRLNGNLLDYALEAKVSAKGMGVPASSASLTGKGELTHFNIQDL
                     SLNTLEGTAKIDGLVDWSEGVAWRSNLKLQHINTKSLLADWPAVLSGSLSSEGYAGRG
                     NSASGWKADVSNIDIKGSLLQKNLHLSGNLKADHQQLLDVPGLSLVYGENKIDLKGVL
                     GEKSDFYADIKAPNLQGLVPNLKASVNGNAKLSGKVSEPNIDLDLVASNVGYEQFKLQ
                     NLTAKGKITTEKTIQGDLTLGLRQFAYNEIKVGNATLLLKGNEENHSLKLSAKGNPVG
                     ADLQISGKFDRLKEIWEGQLSQVSIQSTEFGQFQADKAVNVKYDNKAINANISAHCWH
                     NRKINLCFPTAFNAGVEGKVPFEIRNFDLAVLQQFLEKNSQLSGIVNAKGDAAWFKNK
                     QPQVNLDLTSNAIKFIQKMEGGKSFPLTVSPLKIDLKMSDNNLTLKSDLRVENNGRLS
                     TDLVMKDLSKARALSGNIHLDQLSLKLIRPLLERGDSVDGNVNARLTVSGTATSPLLH
                     GNLNVTELKARSVTMPFDITGGNLAMNFHGATSTLSGKLQTKQSELRLEGDADWRDLN
                     AWKTRVHAQANRFQVDIPNIAKVAVSPDIQVTATPTLLTLSGNVDIPWARIEVEQLPE
                     SAVSVSGDEVIMDGSVKNKVPFSQRNIPAQTSGGMAINADIKINIGNDVKLKAYGLNS
                     NVNGLLSVRQGKQGLGLYGQVHLREGRFAAYGQDLLIRKGDISFAGSPSQPTLDIEAI
                     RNPEAMEDPTITAGVRVTGLADNPTVKVFSDPAMSQNEALSYILTGRSLESSGDTSSS
                     NAVAAALLSMSLSKSSKLVGDVGSTFGLKDLSVTTAGIGDNTKVEVSASLAPKFRVKY
                     GVGIFAPLTELTLRYNLTPRLYLQWVSSINQAVDLMYRFEFDELF"
     gene            complement(11780..13615)
                     /locus_tag="PXD66_000793"
     CDS             complement(11780..13615)
                     /locus_tag="PXD66_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor"
                     /protein_id="HDL6319117.1"
                     /translation="MNTKLHFLTFCCLVAFASMAQANQEQRLSDVDRERVENLQEKLA
                     EDSDDEPEIEPLVTLEVKGIQGKDAKKNVELYLDQIANEYADGSERYQYLVQTTVDKA
                     LRALGYYNSRYQFVQLPQEGKKPLLVLNVELDKETVKIDETDIVLQGDAKTDEDFAHL
                     VSKAPKQGSRLNHKTYDNFKGDIESTAFSKGYFDGHWLYHRLEVYPTDHKADWRLGYD
                     SGKRYHYGQIRFVDSQIKEEYLQNILKIHSGEPYLANDLSILTQDYLSSNWFSSVMVE
                     PHLNEQDKLVDLNVLFQPKKRNEVEIGIGYETEVGPRLQLNWKKPWLNSRGHSIESRT
                     YVSKPEQTFEFGYNIPLREDPLHYYYQLSGTLENEDQNDTKSTAATLGLQRFWTHETG
                     WSFSVGLKARYDSFTQANDRFKTLLVYPTASLNRTRTDGKPYPLWGDTQKLTVNWGSR
                     AVGSDVNFYSYKASTSWARTYFDNHRFYLRAELGYLKANQFERIPPALRYFAGGDMSV
                     RGFGYKDISPRDPSNGKRVGGSHLATGTVEYQYQVYPNWWGAIFYDAGLASNKFKTQD
                     LYYGTGLGVRWASPIGAIKFDVATPVKSPDNSKGIQIYIGLGSDL"
     gene            complement(13627..14472)
                     /locus_tag="PXD66_000794"
     CDS             complement(13627..14472)
                     /locus_tag="PXD66_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HDL6319118.1"
                     /translation="MFKQINKRLNFNIFLQMFVLFTPLVQAAQMAIVIDDVGYRIKED
                     REILALPKAVSVAIIPVAPYATERAKDAYNQKRDILIHLPMEPKSKQPIEEGGIHIGD
                     NEEKIRKLIHTSRGQVPYAIGLNNHMGSGATSDSATMQHLLKVLKENTLFFLDSKTIG
                     SSVAAKTARQFGINTLERDIFLDDSDLLADVQKQFAHAINHARKNGVAVVIGHPRKNT
                     ISVLKQNLAQLPQDIELVSVGNLWRNEKTVPDKPFIMLFEQEPALSSVPPYDSIPLLR
                     GIPKE"
     gene            complement(14473..15636)
                     /locus_tag="PXD66_000795"
     CDS             complement(14473..15636)
                     /locus_tag="PXD66_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDL6319119.1"
                     /translation="MLVKRINSYCWTAIFSGVISFSTPFSFANNLNQIQQKIQQQTSK
                     INEQKQKRNALQSTLKNQEVEMGKVLDKLQKTEMSLTEVRQTIKATETEIARLEKLEK
                     QQKEKLKEQLDSAYRSGVNPSVLERLMSEEAKNAERMTAYYQHMNNIRIDAIVEIRKT
                     QADLKARRDELKGQQKDHQTQLNEQKKQEKDLKKVQSQRESTLRLIDKTLEQDQSRLE
                     NLRSNEQALKQQLEQAQREAQQAEKREQQREKQAKSGQSKTTENVERTPIKAGRYTMP
                     VSGNIITPFGNNWHGVVIGASAGTSVRAMASGRVIMAQWLAGYGNMVAIDHGNGDISL
                     YGYNQSISVSKGSRVQGGQVIAKVGNSGGQSRSALYFGVTRKGTPINPLNLVK"
BASE COUNT         4376 a         3518 c         2931 g         4819 t
ORIGIN      
        1 gtaatgtccc ttttttgaat gtctactaca aaatgggata ttttacaaac cgattgacta
       61 atgaagcaag aatataatta actacaactg gatatatttt gcaaatttcc cgataaattt
      121 caccgcttgt agataaaaac cgtagataaa aaccatagat aaaaaatagg agctaaacat
      181 ttttagcccc taccgtcatc agaaataaat cccgactaaa atcgccacga acacacctaa
      241 gccgaaatag tggttattta gaaacgcttt aaagcattcg ctacgttctc tttttttggt
      301 cagccaacat tggtagataa acagcgtggc acttaaggct aatacgacaa aatagccgat
      361 gtgataatct tttaccacgc ccaataaccc caataaaatc aaggtgataa actgtaataa
      421 cgcaataatt ttattatcgt attgagcgaa taaaattgca gtggatttaa ctccaattcg
      481 taaatcatcg tcacgatcaa ccattgcata ttgggtgtcg taggcgactg tccacgctag
      541 attggcaaaa aacagcagcc agcattcaag tggaagagtt tccgttaccg caccaaatgc
      601 catcggaatc gaccaaccga atgccattcc aagcactact tgtggcaagt gggtaaaacg
      661 cttcataaag ggataaatca ccgccagaaa aactgccaca aaggaaagca gaaacgcata
      721 gcggtttagc attaaatcga gcagaaaagc gaacaacagc aagatggcaa aaatgacttt
      781 tgcctcagtg gtggtcactc tgccggtagc taatgggcgt tgagaggtgc gtttaacgtg
      841 tccgtcaatt tctctatcgg cataatcatt aatcacacaa cctgccgcac gcataacgat
      901 taccccaagg gtaaaaatgg ctaaaatcgc aacaggcggc attccctctg cggcaataaa
      961 taacgcccat agtgtggggt gtaataataa taacgtgccg atgggtttat cgaaacgcat
     1021 taactgggcg taggcaatcc atttttgttt agaaaaaaga gtttcgttat tcattacaaa
     1081 ttttgacgat aacgtccgat tggcttaaat gagtctgtaa ttctaacggt aaatcttgat
     1141 ctgtaaacaa ataatttact tctttaacat ttcctaaacg tgcaatagct gtacgggtaa
     1201 actttgaatg atcggtcact aaaaccactt gtcgggaaca ttccattaat gcacgcttta
     1261 cctgcacttc gtggtaatcg taatcgagca tagagccgtc catatccacc gcactaatgc
     1321 ctaaaatccc aaagtccaaa cggaattggt taataaagcg aaccgtttct tctccgatta
     1381 gcccgccatc acttcttagc cctccgcctg ctaccgtaat ttggcaatct tcattttgca
     1441 tcagaatatg agccgcattt aaattattgg taactacttt taaattttta tgcgaaagta
     1501 aggctaatgc cactgcttct gaagtcgtgc caatatctaa aaataatgat gctccatttg
     1561 gaatcagttt tgccacctgt tgggcaatcg catttttctc gtgtgagaaa aattgcttgc
     1621 ggtgggtgta atcactattc tccgtattgg aaggagcggc tgccccaccg tgatgacggc
     1681 gaatcagatt attttctgcc atttcattca gatcacggcg aatggtttgc ggactcacat
     1741 tcaattcagc cactaattgc tcggtactca catagccaag ctgattaacc agctcaatga
     1801 tctttttgtg tctgtttgcc tgtttcatat ttcctctttt gcaaataatt tgcagaaaaa
     1861 gaccgcttgc aagcggtctt ttttcagtta aattttacaa tttatcaaca agttcaaccg
     1921 catagccgat atagcttgcc ggtgtcatct ctttcaaacg atttttttca ttttccggaa
     1981 tttccaactt atcgataaac tcacgcattg cggtttcatc aacccgtttg cctcgagtca
     2041 gctctttaag tttttcatac ggtttttcaa tgccataacg acgcataaca gtttgaattg
     2101 gctctgccaa tacttcccag ttttggttga gttcatcacg caagtgttgt tcattcactt
     2161 ctaatttgct aatacctttt aaggtggacg cataagcaat taacgcataa cctaaaccta
     2221 cacctaaatt acgcaacacg gtggagtcag ttaagtcacg ctgccagcga gaaatcggca
     2281 atttttgccc caaatgagcc attaccgcat ttgccaagcc aagattgcct tcggaatttt
     2341 caaaatcaat cgggttcact ttatgtggca ttgtgctaga gccgatttca ccggcaatag
     2401 tgcgttgttt gaagtgattt aaggcaatgt agccccacat atcacgatca aaatcaatta
     2461 aaatcgtatt aaaacgagcc acgcaatcaa agaactccgc aatataatcg tgcggttcaa
     2521 tttgggtggt gtatgggttc cacgtcacac ctaacgattc cacaaattct tggctgaaag
     2581 tgtgccaatc aatctccgga tatgctgata aatgtgcgtt atagttgccc actgctccat
     2641 tgattttagc gagaatttct aaattttcta actgtttata ttggcgttgt aaacgatatg
     2701 ccacatttgc catctcttta cccattgtgg tcgggcttgc cggctgaccg tgtgtgcgag
     2761 aaagcaacgg aatatgttgg taacatttcg ccagctctgc tacagcatca atcacttttt
     2821 tccattcagg taataatact tcttcacgag cggttttaag cattaaagcg tgcgaagtat
     2881 tattgatatc ttcagaagta caagcaaaat gaataaattc attcaccgct tgtaattcag
     2941 gcaacgcttc acatttctct tttaggaaat actccaccgc ttttacatcg tggttggtgg
     3001 tacgttcaat cgttttaatc cgattggcat cttctaacga aaaatcagcc acaatttggt
     3061 ttaggtaatc gtttgctttt tcagaaaaca ctggaacttc attaatttgt gtatgcgatg
     3121 ccaatttttg taaccagcga acttccaccg tcacacggaa ttttaataag ccaaattcac
     3181 taaaaatcgg acgaaggctc gcaactttgt cttgataacg tccatcaatc ggggataacg
     3241 cagttaaagc ggtaagttcc attgttttat attctccaaa taggtaaatg ttcggttaga
     3301 tttgacgata aaatttctgt gcttggttaa aaattttctt gcgtgaaaat aaaaattgcc
     3361 aacggcttcc gccgacttgc tgccacaaaa taccggctcg aataccggct agtagcgagg
     3421 ctcgaatttt attctgtata tccgggcgaa ccaagtaatc ttgcattccc atcacgtgaa
     3481 ttttcgtacc taacgggctg ataatatcgc tataaatagc ggctaaattg gcaatcattt
     3541 gatctgccat cacattatct tcatacaggg ctaactggcg ttccacttgc tgtaaacgct
     3601 gggctaactg ttgttttgct tcagggtttt tattgagttt ttggctaagg gcgagcaggc
     3661 ttatccaata acgtccgact tcggtatcta atttgccgtt accgccgcca gtttgagcta
     3721 atgcggtttc taagcccatt tttaaatggg aaagatcatc gccaaataca cttaacgttg
     3781 attccggctg agtgattaac aggctttcta aagcacactt aaaagcctct ctgtctgcca
     3841 tgcctttatg tgcaaattgt tgcactaacg tcgccgcttg gcaaacgccg gcataagcaa
     3901 gggtaatatc gtggtaatcc gccatctctg taaaattcaa tatgataaaa cattagtggg
     3961 aatatagcat tttttcagct tcttttgaat ggcaaaaggc atttattcta ttgaaaaata
     4021 aatgcctttt cattaatacg caatttgctg caaaaactat cctaccaatc gcccattatc
     4081 tattcgataa accacatcaa atttatccag tccgctttgg cggtgagtga tcataattaa
     4141 ggtgcgatca cagcagtgag tcaaaattag gtttaaaatc tgctgttcgg tttctctgtc
     4201 cagtccttct gttggctcat caagtaagac taattcagca gaactcagga gcaaacgggc
     4261 aagccctaaa cggcgttgtt cgccaccaga aagcggtctg ccgccctcgc ctaaccataa
     4321 atttaagccg tctgtttcaa gcaaatgccc taagcctact tttcttactg cttcttgcat
     4381 ttgttcgtct gtagcttgag cattccccat taataaatta tcttttaggg tttggctaaa
     4441 aatatgcact cgttggctta aggtaactaa ttttgagcgt aacatcggct ctgaatattc
     4501 gtcaatttta caattattca gccaaatttg accgcttgta gggtcgtagt tgcggtttaa
     4561 cagttggaat atggtcgatt ttccgctacc cgttttacct aagattgcca ctttctgccc
     4621 ttttaatacc ttaaaggaaa gattatttag tacgttttgc tcgccgtaat aagcaaagct
     4681 cacgttttca cagcgtacaa gcggttgatt ttggctcaaa ttttgccatt ctgccttgct
     4741 accaaatgtt acattaggct gttgttcggt aatttcacta atccgctcgg cggcggtaat
     4801 cacttgccct aaatgcaaaa aggcgatacc aatcggggaa agaatctccg ccgatgccat
     4861 tacacagaaa atcactaacg caattaaggc ttcagggtat tttgcagtgg gcacattaat
     4921 cgaagttgaa accaaataga tcaccacaca agtcaaaatg ccgttggata acattatcaa
     4981 gctatttgat aagccggaca gtcggctttc tttgctttga gcgttcagcc attgttgttc
     5041 agtttgctgt agtttttcgc tcatttggtt caagttgcca aagagtaaaa attctgcatt
     5101 taactgtacc cactcaataa attggctgcg gtaatttgct cggctttgta ttgcattacg
     5161 ccctaatttc aaacccaatc gataaaatac ggtaggaata acggcaagca ataccaatag
     5221 tgtgccgcaa atgatcagca ttaatggcac ggaaacaaaa gaaagcccga ttgccataaa
     5281 tgcaataatc atcactgcac tgacaaaagg cgagactaaa ttcagatata gggtgtctaa
     5341 cgtatccaca tctgccacca agcggtttaa caattcgctg tttctaaagc gatttagccc
     5401 gcttggactc agtggaatca attttttaaa gacgctagca cgcaaattcg ccaatacccg
     5461 aaaagtcgca tcgtgggtga ctaaacgttc aaagtaacgt gaaattgtcc gccctactgc
     5521 caaccctctc acccccgatg aagggtagaa aaagttaaaa ataagcgcat tgccggctag
     5581 aaatgaggca gctaaaaacc aaccggaaag gctgagcaaa ccgatacttg ccgctaatcc
     5641 taaaattgcc agcacaacac ctaataatag tcgcccaaaa tgggtttggt agagagcaaa
     5701 aaacgggaac aacgatttca ttagatttct ccctgatgca ctaattcatt atgtattaat
     5761 tccgcaaaaa agccggtgtg ttctaattca gtgaaacgac ctttctgaat gatttgccct
     5821 tgtttcatca cccaaatttc atcgcattgg gttaaatctt ccactcgatg agtaatcatt
     5881 agcgttgttt gctctcggct gagattatgc agagcggcta acacttgctg ttccgaatcc
     5941 atatcaaggc ttgcagtcgg ctcgtctaac agtaataatt cgtacgggcg aagcaacgct
     6001 cgtgcaatcg caatccgctg tgcttgcccg ccagagatac cgatattact gtcttgcact
     6061 tgatgctcca accctaaacg tgccacaaat tcatcggctt ttgaaagctg taatgccttg
     6121 gctaattcag aatctgtcgc attagggttg cctattaaaa tattctcttt caaactgcca
     6181 tgcattaatt gcggattttg ccccacccac gctaatttcg ctcgccattg gcttaaattt
     6241 aaatctcgca gttcggtgcc gttaatcgta accgaccctt cataaggcaa gaaaccaagt
     6301 aacatattca ttaatgaact ttttcctgca ccactttgcc caaccaaagc gatatgttgg
     6361 tgtgcattca actcgaaatt taacgcttgg gttaaggctt tgccttgtgg tgagaagatc
     6421 acgcaatctt tggcttgaat cacaagcggt tgattttcaa cattttcttg caaattgcca
     6481 ccttgcgttt tcaccttttc ttttaagaaa gtttcgatat tatctgcggc actaatcgct
     6541 gctgccttgt cgtgataaaa cacgccaagt tcacgcatcg gttggtaaaa ctcaggtgcc
     6601 accattagac aaaagaagcc gataaataag gtcacgcccg taccataata gccaaaatcc
     6661 agctcaccta aaaaaataaa gccaaaatag accgccgtca ccgcaatcga taccgccgtg
     6721 aaaaactcca ataccgcaga ggataaaaac gccattttta gtacgtccat cgtactgatt
     6781 cggaaatctt cggtgctttg gtagatttgt tcagtctgct tttccgcttg cccaaataag
     6841 cgaatggttt ctaaaccttt taatttatct aagaactgtc cgctaatacg ggaaagaatg
     6901 ccgatattac gctgatttgc ctccactgct ttcattccgg ctaaaatcat aaataaaggt
     6961 aataatggca ttgttgcaaa taaaatagcc cctgctgccc agttaatcgg gaatacaaaa
     7021 cagaggatca gtaatggcac tatcagcgat aaaaattgct gtggcaaata gcgtgcgtag
     7081 aaattatgta gattttccac ctgctcgagc attaaggttg cccagctgcc cgccggtttt
     7141 tgttgaatgc tcatcggccc gactgcttcc attttggcta gaatttggcg gcgaagatgt
     7201 aaacgcaagg tttgccctgc tttgaaaccg acacgctcac gcagaaaaat gaagaatgct
     7261 ctggcagcaa aacaacagaa taagagcata atttcaacca gaaagctatt cggtgactgc
     7321 tttccgataa gcattttgtc taacattgtg gcaatcagcc acatctgtcc aatcatacat
     7381 aaactactga aactccctaa cagtatgttt aagtgcatat attttttgat gactttttgc
     7441 tgacttttaa gccatttttg taattcgcgt tggcgttgtt tttccataat tttgtcaatt
     7501 tttttgtttt ttcaaccgct tgtaaatcgg gtttgccatt ctactataaa gcaaaaaggt
     7561 tgttaatggc gaaacattaa caacctttca ttcagcctta actagaatag ctcgtcaaat
     7621 tcaaagcgat acattaaatc taccgcttgg ttaatacttg agacccattg taagtacaat
     7681 cttggcgtta aattatagcg taaggttaat tcggtaagcg gtgcaaaaat gcccacgcca
     7741 tacttcactc tgaatttagg ggcaaggctg gcacttactt ctactttggt gttatcgcca
     7801 atacctgcgg tggtcacgct taaatctttg agtccgaaag tgctaccaac atcacccacc
     7861 aatttactgc tttttgataa gctcatactg agtaatgccg ctgcgactgc attactggag
     7921 ctggtatcgc cactgctttc caatgaacga ccagttaaaa tataggaaag tgcttcattt
     7981 tgcgacattg ccggatcaga gaatactttt accgttggat tgtctgccaa accggtcact
     8041 cttaccccag ctgtaatggt cggatcttcc attgcctccg gattgcgtat agcttcaata
     8101 tctaaggttg gctgagacgg tgagccggca aaactaatat cacctttacg aatcagcaag
     8161 tcttgaccat aagcggcaaa tcgtccttca cgcaaatgaa cttgcccgta aagccctaaa
     8221 ccttgtttgc cttgcctgac agacaataag ccattcacgt tactgtttaa accataggct
     8281 ttgagtttga catcattacc gatgttaatc ttaatatctg cattaattgc cattccacct
     8341 gaggtttgag ccggaatatt acgttggcta aacggcactt tatttttcac agagccgtcc
     8401 ataatcacct catctccact tacactgacc gcactttcag gtaactgctc gacttcaatt
     8461 cttgcccaag ggatatctac atttccactt aaggttaaaa gagttggcgt tgccgtcact
     8521 tgaatatccg ggctaactgc cacttttgcg atattaggaa tatccacttg gaaacggtta
     8581 gcttgagcgt gaacccgtgt tttccacgca tttagatctc gccagtctgc atcaccctcc
     8641 aaacgcagtt cgctttgttt ggtttgtaat ttaccgctta aagtcgatgt tgccccgtgg
     8701 aaattcattg ctaaattccc acctgtaata tcaaacggca ttgtaacaga gcgagctttt
     8761 aactctgtaa cgtttagatt tccgtgcaat aacggagagg ttgcggtgcc gctcacggta
     8821 agacgagcat tcacattgcc atcaaccgaa tcgccccgtt caagtagcgg tcgaatcagc
     8881 tttaagctta attggtcaag atgaatattg ccggataacg cacgagcctt acttaaatct
     8941 ttcatcacta aatcagtcga aagtctgccg ttattttcga cccttaaatc agatttaagg
     9001 gttaaattat tgtcagacat tttcagatca attttaagcg gtgaaacggt gagtgggaaa
     9061 ctttttcccc cctccatttt ttgaataaat ttaatcgcat tagaggttaa atccagattc
     9121 acttgaggct gtttgttttt aaaccaagct gcatcgcctt tggcattcac aatccctgaa
     9181 agctggctgt ttttctccaa aaattgttgc aataccgcta aatcaaagtt acggatttca
     9241 aaaggcactt taccctcaac accggcatta aatgcagtcg ggaaacaaag attaattttc
     9301 cggttatgcc aacaatgtgc ggaaatattg gcgttaatgg ctttattgtc atatttcaca
     9361 ttcactgctt tatctgcttg gaattgacca aactcggtgg attgaatcga aacttggctt
     9421 aattgccctt cccaaatttc tttcaaacgg tcaaattttc cagaaatttg caaatcagca
     9481 ccaaccggat tgccttttgc acttaatttc aagctatgat tttcttcatt tccttttagc
     9541 aataaagtgg catttcccac tttgatttca ttataggcaa attgacgtaa ccccaaggtt
     9601 aaatcccctt gaatggtttt ctcagtggta attttgcctt ttgcggttaa attttgcagt
     9661 ttaaattgct catagcctac atttgaggcc actaaatcta aatcaatgtt aggttctgaa
     9721 accttacccg ataatttggc attaccattc acgctggctt ttaaattcgg cactaaccct
     9781 tgcaaattag gggctttaat atccgcatag aaatcagatt tttcgcctaa cacgcctttt
     9841 aaatcgatct tattttcacc atacaccagc gataagcccg gaacatctaa aagttgttga
     9901 tgatcggctt ttaaattacc gcttaagtgt agatttttct gtaataacga acctttaata
     9961 tcaatattcg agacgtccgc tttccatccg ctagctgaat tgccccgtcc tgcataacct
    10021 tcggaagata aagaaccgga caataccgcc ggccagtctg ccagtaggga tttagtgtta
    10081 atatgctgaa gtttcaaatt tgaacgccac gcaacgccct cgctccaatc cactaaaccg
    10141 tcgatcttcg ccgtaccttc cagcgtattg agacttaaat cttgtatatt aaaatgggtt
    10201 aattcccctt tgccagttaa actagccgag cttgcaggca cgcccattcc tttggcactg
    10261 actttagcct ccaaagcgta atccaggaga ttgccattaa gcctcatggc gatattctct
    10321 aaactcagca catcaccatt ttgctgagga gcaaacggat aacgcattga ctcactcttc
    10381 agcgataaat caaagggagt tttcgcctga gccagtgcaa cattgccctc caacgaggcg
    10441 ctaattgcac cagccgtctt cacttttagg ctggttttgt catatagctc accggaaaga
    10501 gaggcttcaa tttgattgaa cggcaatttt actttcgccg tttttgcctc gtgaccactt
    10561 agcgtccaac ttagcggata attatcttgt aacgataaag aaccgcttgc ggttagatcc
    10621 ccttggtcgc ttttgaaatc taaacttttt aggttgatat gctgatcttt tgcttgagcc
    10681 tgaagctgta aagaggcaac atttaacaat gagatcggct caatcaaatt ccctttttca
    10741 tctttagctt tttgagcaat cgctacattc gcaatatcaa tctgttcaat attgagatca
    10801 agaggcaaac taaagcgaat atttttatcc aataacggct gtgctaattg tgctttaatc
    10861 gctgcccaat ccactttatt atccacctgt tcggcctgtg caacagtttt tctctgtaat
    10921 tggtgaaagc cctctacgac tttttcactg gttttttcca ccgcctcttg aatcacttta
    10981 tcagcaatct cttgtggatt tgtcgctgat aacgcctctg tttttgcatt tttttgtgca
    11041 aaatcgaccg cttgttcagt atcagtttcg tctaacttta aggctaaaag cagatttagc
    11101 ccctctaact gtgtcgggtg aacggtaata gcccgccctt taccagaaag tgcagtgtgg
    11161 aaatgattaa gatgaatatc catatcatca actttgacct tgatattctc taaattgata
    11221 ttttgagctg aaatccccaa tggcaaattt aattcggtga aaggttcggt ctcttctttt
    11281 ggctgagaag ggggtaattt agtcgtatca atcaacacct ctgtatcttt aaggctgaga
    11341 ttattcaaac acatttcata gtggagcaag cagctaaaac caatatgcag atctgcttgc
    11401 ccaaccgtta cattcacacc gtccatcaca aatcgggcat cagaaagctg taacccatct
    11461 tgcaggctac cttgcacttg cccaagttgt aatggctcta cgaatttatc tacccagctt
    11521 aacactttac gttgcccgta acctgtgcat agaaagagaa tggcgagtaa aagtagaata
    11581 aacgtcagac aaaagccgtg cttcaaaaac cgccactttg attttttagg ctgtgttgct
    11641 tgtggctctt gtgctgagtc cgtttgattt gtggctgttt gtttattttc gtcagtcata
    11701 atcgtgcata tttatgaggt gataaaaagt aaacaagcgg tatgcgaaat tttactgaaa
    11761 ttcgaccgct tgttttcatt tacagatcgg aacctaaacc aatataaatt tgtattcctt
    11821 tggaattatc tggtgatttt accggcgtgg cgacatcaaa tttaatcgcc ccgataggtg
    11881 atgcccaacg cacccctaag cccgtaccgt agtagagatc ctgagttttg aatttattcg
    11941 atgccaagcc tgcatcataa aaaattgcac cccaccaatt agggtaaacc tgatactgat
    12001 attctactgt gcctgtggct aaatgagagc ctccgacacg tttgccatta ctcggatcac
    12061 ggggggaaat atccttatag ccaaagcccc gcacactcat atcgccaccg gcaaaatagc
    12121 gtaacgccgg tggaatacgt tcaaattgat tggctttcaa ataccccaac tccgccctta
    12181 aatagaaccg atggttatca aagtaagttc tcgcccaaga tgtcgaggct ttataactat
    12241 aaaagtttac atctgaacct actgcacgac tcccccagtt taccgttaat ttttgtgtat
    12301 ccccccataa tgggtaaggc ttgccatctg ttctggttcg attcaatgat gccgttgggt
    12361 aaactaataa ggttttaaat cgatcattag cttgagtaaa agaatcgtaa cgggctttta
    12421 aaccaacaga aaatgaccaa ccggtttcgt gagtccagaa acgttgtaag ccaagggtgg
    12481 cagcggttga ttttgtatcg ttttgatctt cgttttctaa agtgccggat aattggtaat
    12541 aataatgtag cggatcttct ctaagtggaa tattataacc aaattcaaat gtctgctccg
    12601 gcttagaaac gtacgttcgg ctttcaatac tatgacctcg gctatttaac caaggtttct
    12661 tccagttaag ctgtaacctt gggccaactt cagtttcgta gcctatccca atttccactt
    12721 cattgcgttt tttaggctga aataacacat ttaaatccac taatttatcc tgttcattta
    12781 aatgtggctc aaccatcact gatgaaaacc aattactgga aaggtaatct tgggttaaaa
    12841 tcgaaagatc gttagctaaa tatggctcac cggaatgaat tttaagaata ttttgcaaat
    12901 attcttcttt gatttggcta tcaacaaacc ggatttgtcc ataatggtat cgttttccgc
    12961 tatcatagcc taaacgccaa tccgctttat gatcggtcgg ataaacttcc aaacggtgat
    13021 aaagccaatg accgtcaaaa taccctttac taaaagcggt actttcaata tcacctttaa
    13081 aattatcata ggttttatgg tttaaacgac taccttgttt tggggcttta ctgactagat
    13141 gagcgaaatc ttcgtccgtt tttgcatcgc cttgcaaaac aatatcggtt tcatcaattt
    13201 ttaccgtctc tttatctagc tcaacattta atactaagag cggtttcttt ccctcttgag
    13261 gtagctgcac aaattggtat cggctattat aatagcccaa ggctctcaga gctttatcta
    13321 ctgtggtttg caccaaatat tgataacgct ctgaaccatc tgcatattca ttggcaattt
    13381 gatctaaata tagctccaca tttttcttgg catctttacc ctgaatgcct ttgacttcta
    13441 aagtgactaa tggctctatt tctggctcat catcactatc ttccgctaat ttttcttgca
    13501 gattttcaac tcgctcacga tctacgtcag acagcctttg ctcttgattt gcttgtgcca
    13561 ttgaagcaaa agccactaaa cagcagaatg ttaaaaaatg tagttttgta ttcattagta
    13621 acctttttat tcttttggaa tgccacgcaa aagcggaata ctgtcataag gcggaacaga
    13681 acttaacgct ggctcttgct caaagagcat aatgaatggt ttgtcaggca ctgttttttc
    13741 attacgccac aagtttccaa cactcaccaa ttcaatatct tgaggtagtt gtgctaaatt
    13801 ttgcttcagt acactaatcg tattcttgcg agggtgtcct atcaccaccg ctaccccgtt
    13861 ttttcgtgcg tgattaattg catgtgcaaa ctgtttttgt acatcagcaa gcagatcgct
    13921 atcatctaaa aaaatgtctc tctccagcgt attaatacca aattgacgag ccgttttagc
    13981 cgccacacta ctgccaatag ttttactgtc taaaaagaaa agcgtatttt cttttaacac
    14041 tttcaacaaa tgttgcattg tagcagagtc tgaagttgct ccactgccca tatggttatt
    14101 taaaccaata gcatatggta cttgaccacg agaagtatga ataagtttcc gaattttctc
    14161 ttcgttatca ccaatatgaa tacccccctc ttcaattggc tgtttcgatt taggttccat
    14221 tggcaaatga atcaaaatat cccgtttttg gttataagcg tcctttgccc gttctgttgc
    14281 atagggtgca acaggaataa tcgccacgct taccgccttg ggtaaggcta agatttctcg
    14341 atcttctttt attcgatagc ccacatcatc aattacaatc gccatttgag ctgcttgtac
    14401 aagcggtgta aaaagcacaa acatttgcaa aaaaatgtta aaattcaacc gcttgttaat
    14461 ttgtttaaac atctatttca ccaaatttag aggattaatc ggcgtgcctt tacgggtaac
    14521 accaaaatag agtgcagaac ggctttgtcc cccactgtta ccaaccttcg caataacctg
    14581 tccgccttgc actcggctac ccttgctgac tgaaatagat tggttatagc cataaaggga
    14641 aatatcacca ttgccgtggt caatcgcaac catattgcca taccctgcaa gccattgagc
    14701 cattatgact cgcccgctcg ccattgctct aaccgaagta ccggcagaag caccaatcac
    14761 tacaccgtgc caattattac caaaaggagt gataatattg ccagacaccg gcatcgtata
    14821 tcgaccagcc ttgatcggtg ttctctcaac attctcagtt gttttggact gaccggattt
    14881 tgcctgtttt tcccgctgtt gttcacgttt ttctgcttgc tgagcctctc tttgcgcttg
    14941 ttctaattgt tgttttagtg cctgttcatt gctgcgtaaa ttctctaatc ggctttgatc
    15001 ctgctcaagc gttttatcga ttaaacgtaa ggtactttca cgctggcttt gtactttctt
    15061 taaatctttc tcttgtttct tttgttcatt aagttgcgtt tgatgatctt tttgctggcc
    15121 tttcaattca tcacgtctgg ctttaagatc agcttgggtt ttacgaattt caacgatcgc
    15181 atcaatgcga atattattca tatgctgata ataagcggtc attctttccg cattttttgc
    15241 ctcttcagac attaaacgct ctaagaccga agggttcacc ccagaacggt aggcagagtc
    15301 caactgctct tttaattttt ctttctgctg tttttccaac ttttctaaac gggcaatttc
    15361 agtctcagtc gctttaatgg tctggcgaac ttccgttaaa gacatttcgg ttttttgtaa
    15421 tttatccaac accttcccca tttctacttc ttggtttttt agggtagatt gcaacgcatt
    15481 acgtttttgc ttttgttcat taatttttga ggtttgctgt tggatttttt gctgaatctg
    15541 attaagatta tttgcaaaag aaaaaggggt agaaaagctt attaccccac tgaaaatagc
    15601 agtccaacag taagaattta ttcgtttcac cagcatacct ttgt
//
LOCUS       DANLXF010000042        15623 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.042, whole genome shotgun sequence.
ACCESSION   DANLXF010000042 DANLXF010000000
VERSION     DANLXF010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 15623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15623
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.042"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            6..2951
                     /gene="ptrA"
                     /locus_tag="PXD66_000796"
     CDS             6..2951
                     /gene="ptrA"
                     /locus_tag="PXD66_000796"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HDL6319120.1"
                     /translation="MKLSFLTQFVAAAFALNVMTYPAFAEVAKNSEKTTASQPQSQLG
                     FELIKQSINKSPNDKAIYQGIKLDNGMEVLLISDSQANKSLMSAIIPIGSMDDPISQQ
                     GLAHYLEHMILMGSKNYPETNSLDGFLTKNGGMNNASTASHRTAYYLQVNNEAFSEAV
                     TRLADTLAFPLLLETNAKKEVNAVNAEMVRAKSHDGHLLHSVNLATANPAHPATKFAV
                     GNKETLSDKPDSKLQTELEQFYKQHYSANLFKVVLYSNQSIEQMAKLAANTLGKMENK
                     HFSIPQTAEPRYRDEDKSVFIQYKPVKSNKMLVIGFDFPNDEMQFKHKTGKYIAYMLN
                     NNTDGTLSDYLIKQGLSDGGIEAVSSANIGRDRGSFDIYIELTDKGLSEQDQIISLVF
                     QQIEKIKQEGIQESYFSEVKESLKQDFQHLQVEKNMDFVEFIADQMLHFPLEHVIDQP
                     YLTETMDNEAIKAKLAAMNIDNARIMLVSDTAKTDKKTPYFEAGYSVAKITQEQKQKW
                     LDFSKNPQLNLPELNPYFTTDFTLNKVETGLTKPKLINDQAGNLIYTMPSVYFTNEPK
                     AKIGVGFNIRPRKDDLKSLTAASILGYMNNLAQTKLDFQSSVAGMNLSLAPAANGLAL
                     SAEGYTQNLAKLMLDSVKQFSRFEFNEDVLAQAKQRLHQELDRNEKENSLNQANAALS
                     RFASYPYFDVPKQREMIDSITLADIEQLRQRILQQSSGFKLLSVGNLSAEQVSALAED
                     LQAVIKNQKSANATGQYVDVSTSQRKINVIKSVPNEDNALLMAFMPNGYNELESLTRA
                     KLLRSIISRWYFDDLRTDKQLGYVVYATDNIIGKTAGIQFMVQSPNTTPAGILEHNER
                     FFTQSFERLKNLSDEEFGKYRDSLVEKLQYKPESLAEEFSEFGSDFSRNNPKFDRLQQ
                     VIELTKALTKADIVAFYQKAVIEKQGFVFVSQAVGTKTKAEDVAKFDGFEQIESIEKL
                     QREFEIKDY"
     gene            complement(3000..4646)
                     /locus_tag="PXD66_000797"
     CDS             complement(3000..4646)
                     /locus_tag="PXD66_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL6319121.1"
                     /translation="MKFPTLTKISASVLAVLALAACDDKSNENKPTAAAAKPQTEKTF
                     VNCVSRSPTGFSPILMMDGLSYNASSQQIYNRLVEFVPGTTDIEPALAESWEISEDGL
                     TYTFKLRKGVKFHSNKDFTPSRELNADDVVFSFNRQLDKNHPYHTVSNGTYPYFNAMK
                     FPTLLKSVEKVDDSTVRITLTKRDASFLSSLGMDFTSIYSAEYADKMMKAGIPEAVDT
                     TPIGTGPFVFSGYVLDQASRYVANKEYWKGKPDIDRLIFEIVPDATTRYAKLQSGQCD
                     LMDFPNATDIEKMKNDPKINLLSQPGLNIAYVAFNTEKAPFDNVKVRQALNLAVDKKA
                     IIDVVYQGNGFAAKNPLPPTIWGYNDSLADSEYNVEKAKQLLAEAGFPNGFETELWVQ
                     PVVRASNPNPRRMSEIIQADWEKIGVKAKLVTYEWGDYIKRTKAGELTAGTYGWSGDN
                     GDPDNFLSPLFGTPNIGNSNYARFTNPELDALLEKALGSSDKAERTKLYEQAQVILRE
                     QAPWINVAHSINFAPTSKRVEGYKQSPFGYTYLYPVKIVD"
     gene            4722..6812
                     /locus_tag="PXD66_000798"
     CDS             4722..6812
                     /locus_tag="PXD66_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HDL6319122.1"
                     /translation="MIFANQSIELDTVNVIATRDPSKFADTPQKQTKDALLVKQATSV
                     AAALKALPNVDVQGGARAIVQKPSIRGLSDNRIVQVIDGVRQNFDLAHRGSYFVPMSL
                     IQEIEVIKGPSSSLWGSGALGGVVAMRTPNALDLLKNNDKFGAKIRQGYQTANNLSET
                     EASVFAANDRFDALLSGFYNNADNLRSGEGKELNNTGYKQAGGLVKFGWQINDTHRLE
                     LSHRHSQFKQTAPGNNEVENQFTDNDVSAQISAWHAANPGNPSNPVYLAKMLAFYGQL
                     HSSLGSVSYLSDQKITDKSTSLNYYFNPSNPYLSTQVTLYSNSTTENEHRKISGVEDQ
                     TKLSTRGVNIRNHSELSWFSLVYGVDYMLDKVATQRGRNNTDAQYRANPYDAKSKTTG
                     AYLIAHIPLFDEKVVFSPSVRYDRFDTSSETVKYKDSHWSPAAKLTWKATNWLDLTAK
                     YNEAFRAPSMQERFVSGSHFGTTVRGLPINNIFLTNPNLRPETAKNKEVTFNVHFDNV
                     ISQGDKFNFEATYFRNDVKDLINLQVFKANPTSPMEFIPTRSQYQNIANARLSGIELQ
                     TRYQTERLAVFANYGSTKGKDKDSGEALSNIAASKIGFGVNYAVVQDKFTVGANVTRY
                     QAQHRVPKNHGVTYQGYTLTDLHATYAPLKGEWKNLRLDVAVENLFDKKYQPAFSLME
                     GSGRNVKLSAAYSF"
     gene            6883..8571
                     /locus_tag="PXD66_000799"
     CDS             6883..8571
                     /locus_tag="PXD66_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HDL6319123.1"
                     /translation="MKFYPSLIASAVSFAVVLSGQAAVNVPDAGSLQRELDQRQAQQK
                     VKPSGEWFNQAAKAPYTATDGQELTFPLTQVQVFDRTNQEISTPELAPILANYVGKAV
                     SLTDLSNLANEITEFYRSHNYLVAKAILPPQEIEQGKVKILLFKGNVGEVRLQNQSAL
                     SDKFVARLANTTVNGSEFILKDELEKFALTMNDVPGVNTGLELSAGKQAGEANLLIKL
                     QNAERFSGYVSADNQGNKNTGRYRLATGIKANNLIGWGDELKVDLMSSNNANLKNARL
                     DYSSLIDGYATRLGLTANYLDYELGGNFKALQSQGHTHNLGAYVLHPTIRLPDFRLNT
                     KVSFNHQRLTDKQQAVNVAQKRQVNSLTVGINGSWNSLKDGTSYFSLSALFGNLANHT
                     NEQAHYVATDFQPKSRFTVYQYSLSHEQALPKSFAFNLGVNGQFADKTLDSSQKMLLG
                     GLYGVRGHQAGVASVDEGHLVQTELKHYLPLFSKSLLTSSVFYDYGWGKYYKHSGFLA
                     RGVNNSVKLQSIGAGLSLSEAGSYAINASVAKPLGKKIENADKHQFWFSAIKTF"
     gene            8583..11399
                     /locus_tag="PXD66_000800"
     CDS             8583..11399
                     /locus_tag="PXD66_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HDL6319124.1"
                     /translation="MFKRNAISLCILTACSGLAYANTADLPQGERVRVGTATIERTTD
                     KMTVNQSSNKVQIDWNSFDIGKNKEVEFKQPTEMAVAYNRVTGGNASQIQGKLTANGK
                     VYLANPNGVLITKDAEVNVGGLLVTTKDLEKISENGTKDKFSRKLAKEGKILNQGKIT
                     AKGERAFVVLVGDDVANEGEINAKNYYHKETKKVTECYDDSWSLVKQCYDFDKEIVKS
                     TSGEVHLSSGENVTFTLSDRYIDVALDDNTVNSIIKNDGAIIANGDITLTAKGRNQAL
                     DSLIINNGVLQANKVQNKNGTIILSAGETQLHEKSQIKGEKVSFAEASYYGPTEDIKV
                     VSKTGSKVTAPVIDFKGKTVNIQGEFGREDSTDLYNDELQILKTTVNIEVPNTEDIRI
                     ADTDNGGSGSFIQAGALASLLANNGRVNVQGKGFDVSGKLNVNAFKQTDSVLKFINQA
                     GINIHNADIHANGRLFFVTNLPNDTDFQSNIRITDSKINLGNGSMGFGRSSKRWEMHD
                     VVRRADTELRKKFNLDMKNVELNQVDDFVVAGGFQQVNLDNVKARGKTNFYLDSGNSR
                     MFVGEQIYEYGVTDIEKRTQLANLDQRRKRWNSDNYDETDLNWEYNQRFDGFAAKHNH
                     RAPIADTEINIANSDISLDNGFVHLMAEKINLDDSKINITFNQDNSLDESTQINRLGL
                     NGKVSMKNSHINVVGDEKDGISPARHYATMFLVGELLGEKSSIFLKSHQGAVLRTDSN
                     VTIAGKNSKDDLKITAITTGKKMGNEIIGPDGLPTSEDNDANASNTAFAIGYEPNATA
                     IVENASITALAPNGGLAYTGTSKTQIDAKDNATFTFFEKPRAGYDKLLGDIKGNPDKL
                     TPRDFARLYDNIKGIKASSLSEQQLGLADTAKKTSEHTLANSLDVENLVSVTVCDEQN
                     KCEERMLGNKNTNAKVIVGDIKFE"
     gene            11522..11653
                     /locus_tag="PXD66_000801"
                     /pseudo
     CDS             11522..11653
                     /locus_tag="PXD66_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587011.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(11708..12469)
                     /locus_tag="PXD66_000802"
     CDS             complement(11708..12469)
                     /locus_tag="PXD66_000802"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDL6319125.1"
                     /translation="MKLNQAQIQNALICGQAVVFDEIDSTNEYLLTHHQRLENGTVCL
                     AEKQTAGRGRRGRTWYSPESENLYFSILWHYPMEQAADLPPLSLVVALIIAESLTVQN
                     VKDIQIKWPNDIYYQGKKMGGILLETQANRNGLDVVIGIGLNLGMAKVDENIVTQAWA
                     DLSQYHFNRNELVCRLAYELQKNLKIYPLVGFAHYAERWQSFDLFRHKAVKLITEAEE
                     IHGISQGINEQGELILKQGDNLRYFGIGEISLRAD"
     gene            complement(12481..13512)
                     /gene="murB"
                     /locus_tag="PXD66_000803"
     CDS             complement(12481..13512)
                     /gene="murB"
                     /locus_tag="PXD66_000803"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDL6319126.1"
                     /translation="MMKHSLTPFHTFHLPAKATQIIEFTTVEQLLSEWQKAFNAQLPI
                     LILGQGSNVLFLEDFDGVVLVNKLKGITHHEDEHFHYLHVQGGENWHELVKWTLAHNI
                     AGIENLALIPGVASSAPIQNIGAYGVEFEQVCDFVEVLNLRSGELFKLSKSECEFGYR
                     ESVFKHQYRDEFAIISVGLKLAKAWKPVLSYGSLAQLNPETVTPIQIFDEVCAVRSSK
                     LPNPDEFGNAGSFFKNPVISNEQFAPIQAKFPAIPHYPQADGTVKLAAGWLIDQTGLK
                     DLQIGGAAVHTQQALVLINKENATGQDVLALAKTVRQRVKEKFGVEIQPEVRFIGKNG
                     EVDSEKITR"
     gene            complement(13616..14290)
                     /gene="crp"
                     /locus_tag="PXD66_000804"
     CDS             complement(13616..14290)
                     /gene="crp"
                     /locus_tag="PXD66_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010787261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HDL6319127.1"
                     /translation="MQDMTLSTAPLENVTPPPSIHDPAVEWFLTHCHIHRYPAKYTLI
                     HAGEDAESLFYIVNGSVSVYIKDDETKEMLLTNLGTGEFVGELSLFEEKVQQRTAYVR
                     TKGPCEIAEISYKKFKQLVHLNPDILMYLSAQMARRLRQTSRQVGNLAFLDVAGRIAQ
                     TLLNLAKMPDAMTHPEGMQIKITRQEIGQMVGCSRETVGRIIKMLEDEGLISAHGKTI
                     VVYGAR"
     gene            complement(14352..14570)
                     /locus_tag="PXD66_000805"
     CDS             complement(14352..14570)
                     /locus_tag="PXD66_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HDL6319128.1"
                     /translation="MIIPWQELEESTLHNILDSFILREGTDYGERELSLAEKRERLLT
                     QLKADKVVIVWSELHESLDIKDKKSFLG"
     gene            complement(14573..15178)
                     /gene="slmA"
                     /locus_tag="PXD66_000806"
     CDS             complement(14573..15178)
                     /gene="slmA"
                     /locus_tag="PXD66_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HDL6319129.1"
                     /translation="MTEPKIKMPKKSVVERRQQVLEVLISLLNSEEGMQRITTERLAA
                     AVGVSEGALYRYFPSKTKMFEALIEKIEQTLTSYIHANKRQDSSANSVRAILYVILEF
                     ARKNPGVTRILTGHALMFEEELLKARVAKFFDNLELQFANILQLSKLRERKSFEDERA
                     LAGYLVNFCEGQFLRLVRSNFSYNQHQHFEKQWAFIKPLFD"
     gene            complement(15190..>15623)
                     /gene="dut"
                     /locus_tag="PXD66_000807"
     CDS             complement(15190..>15623)
                     /gene="dut"
                     /locus_tag="PXD66_000807"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014550997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDL6319130.1"
                     /translation="LDSRIGNEFPLPAYATEGSAGLDLRAMLNEPLTVKAGETVLIPT
                     GISIYIADPNLAAVILPRSGLGHKNGIVLGNLVGLIDSDYQGELKVSLWNRSQADFTV
                     NVGDRIAQLVFVPVVQASFNIVQEFEQTDRGEGGFGHSGKQ"
BASE COUNT         4668 a         3306 c         3430 g         4219 t
ORIGIN      
        1 gatgtatgaa attatctttt cttacccaat tcgttgcagc ggcttttgcc ctcaatgtga
       61 tgacttaccc tgcatttgcg gaagttgcaa aaaattctga aaaaacgacc gctagtcaac
      121 cccaatccca actcggtttt gagctgatta aacagagcat taacaaaagc ccgaatgata
      181 aagcgattta tcaaggtatt aaacttgata atggaatgga agtgctactg atttccgaca
      241 gtcaagcgaa taaatcgttg atgtctgcca ttattccgat tggctcaatg gatgacccga
      301 tttctcagca aggtttagcc cactatcttg agcatatgat cttgatgggc tcgaaaaact
      361 atccggaaac caacagctta gacggttttt taaccaaaaa tggcggtatg aataatgcct
      421 cgactgcttc tcaccgcacg gcgtattatc tacaagtcaa taacgaagcc tttagcgaag
      481 cggtaacacg actggcggat acgttagctt tcccgttatt gctggaaacc aacgccaaga
      541 aagaggttaa tgcggtcaat gcggagatgg tgcgagctaa atcgcacgat gggcatttat
      601 tgcacagtgt taatttagcc accgcaaacc cggctcatcc tgctaccaaa tttgcggtag
      661 gcaataaaga gacgttatct gacaagccgg atagcaaatt acaaaccgag ttggagcaat
      721 tttataaaca gcattattct gcgaatctgt ttaaagtggt gctttattcc aatcaatcga
      781 ttgagcagat ggcaaaattg gcagccaaca ctttaggcaa aatggaaaac aaacatttca
      841 gcataccgca aaccgctgag ccacgttatc gtgatgagga caaatcggta tttattcaat
      901 ataagccggt gaaatccaac aaaatgttag tcattggctt tgattttccg aatgacgaaa
      961 tgcagtttaa gcacaaaacc ggtaaatata ttgcttatat gttaaacaat aatactgacg
     1021 gcacgctttc ggattacttg attaaacagg ggttgtctga cggcggtatt gaggcggtta
     1081 gcagtgccaa tatcgggcgg gatcgaggct cgtttgacat ctatattgaa ctgacagaca
     1141 aaggtttgag cgagcaagat caaattattt cattggtttt ccaacaaatt gagaagatca
     1201 aacaagaagg cattcaagag agctatttta gcgaagtgaa agagagcctg aagcaggatt
     1261 tccaacattt gcaagtggaa aaaaatatgg atttcgtgga atttattgcc gatcaaatgc
     1321 tccatttccc gttagaacac gttatcgatc agccttatct gaccgaaaca atggataacg
     1381 aggcaattaa agcaaaattg gcggcaatga atattgataa tgcccgcatt atgttagtca
     1441 gcgatactgc caaaaccgac aagaaaaccc cttattttga agcaggttat tcggtggcaa
     1501 aaatcacgca agaacaaaag caaaaatggc tggatttcag taaaaatcca cagctcaatt
     1561 tgcctgaatt gaacccgtat ttcactaccg attttacctt aaacaaggtc gaaaccggct
     1621 taactaaacc gaaattgatt aacgaccaag cggggaattt aatctataca atgccgagcg
     1681 tgtattttac caatgaaccg aaggctaaaa tcggcgttgg ctttaatatt cgcccgagaa
     1741 aagatgactt aaagagcctg accgcagcgt ctattttggg ttatatgaac aatttagccc
     1801 aaaccaagtt agattttcag tcttcggtgg cgggtatgaa tttaagttta gctccggcgg
     1861 caaacggttt agccttaagt gcagagggtt atacccaaaa tctggctaaa ttaatgctag
     1921 acagcgtaaa acagttcagc cgctttgagt ttaacgaaga cgtgttggca caggctaaac
     1981 aacgtttgca ccaagagctg gatcgtaacg aaaaagaaaa cagccttaat caagctaatg
     2041 cagcattaag ccgttttgcc tcttaccctt attttgatgt gccgaaacag cgtgagatga
     2101 ttgatagcat tacccttgcg gatattgagc aactacgcca gcgaattttg cagcagagca
     2161 gcggctttaa gttgttatcg gtggggaatt tgtcggctga gcaggtttct gctctggcag
     2221 aggatttaca agcggtcata aaaaaccaaa aatctgcaaa tgctaccgga cagtatgtgg
     2281 atgtgagtac cagtcagcgt aagattaatg tgattaaaag cgtgccgaat gaagataatg
     2341 cgttgctaat ggcatttatg ccgaacggct ataacgagct ggaaagcctt actcgagcca
     2401 aattgctcag aagcattatt tcccgttggt attttgatga tttgcgtact gataagcaac
     2461 taggctatgt ggtttatgcg acagataata tcatcgggaa aaccgccggt atccagttta
     2521 tggtacaaag cccgaatacc acaccggcag ggattttaga gcataacgaa cgtttcttta
     2581 cacaaagttt cgaacgttta aaaaatctat ctgatgagga atttggaaaa tatcgtgata
     2641 gcctagtgga aaaactgcaa tataaaccgg agtctttagc ggaagagttt tctgagttcg
     2701 gttcagactt tagcagaaat aacccgaaat ttgaccgctt gcaacaggtg atcgaattaa
     2761 ccaaagcact caccaaagca gatattgtgg cattttacca aaaagctgtg attgaaaagc
     2821 aaggcttcgt gtttgtcagc caagctgtcg gcaccaaaac caaagctgaa gacgttgcga
     2881 aatttgacgg ttttgaacag atcgaaagca ttgaaaagct acaacgagag tttgagatta
     2941 aggattatta gagagttatt agagagagtt aggggcgaaa aattttcgcc cctcattttt
     3001 taatccacaa ttttcaccgg atataagtaa gtatatccga acggactttg tttgtagcct
     3061 tcaacacgtt tgctggttgg ggcaaagtta attgagtgag ccacgttaat ccacggagct
     3121 tgctctctta agataacctg tgcctgctcg tataacttag tacgttctgc tttatcagat
     3181 gagcctaatg ctttttctaa taacgcatct aactcagggt tagtaaagcg agcatagtta
     3241 ctgttgccga tatttggcgt gccgaatagt ggcgataaga agttatccgg atcgccgtta
     3301 tcgcccgacc aaccgtaagt tccggcggtt aattcaccgg ctttggtacg cttgatgtaa
     3361 tcaccccatt cataggtgac taattttgct tttacgccga ttttttccca gtcggcttga
     3421 ataatttcag acatacggcg tgggttcggg ttagaagcac gcaccacagg ctgcacccat
     3481 aattcggttt caaaaccgtt cgggaagcct gcttcagcta atagttgctt cgctttttcc
     3541 acgttatatt ctgaatccgc taagctgtcg ttatagcccc aaatggttgg cggaagtggg
     3601 tttttcgccg caaaaccgtt accttggtaa actacatcaa taatcgcttt tttatccact
     3661 gctaagttta acgcttggcg aactttcacg ttatcaaacg gagctttttc ggtattgaat
     3721 gccacataag caatgttcaa acccggttga gaaagtaaat tgattttcgg atcatttttc
     3781 attttctcaa tgtcggttgc atttgggaag tccattaaat cacattgacc ggattgtaat
     3841 ttcgcataac gggttgttgc atccggcacg atctcaaaaa tcaaacggtc aatgtcaggc
     3901 ttacctttcc aatactcttt gtttgctaca taacgactgg cttggtctag cacataaccg
     3961 ctaaacacaa acggacctgt gccgattggc gtggtatcta ccgcttcagg tatgccggct
     4021 ttcatcattt tatccgcata ttccgccgaa taaatggagg tgaaatccat accaaggctg
     4081 gataagaatg acgcatcacg tttggttaag gtaatacgaa cggtgctgtc gtccactttt
     4141 tccaccgatt tcaataatgt cgggaatttc attgcgttga aatacggata agtgccgtta
     4201 gagacggtgt gatacggatg atttttatct aactgacggt taaatgaaaa tactacatcg
     4261 tccgcattca attcacggct tggagtgaag tctttgtttg aatggaattt cacgcctttg
     4321 cgtaatttaa aggtgtaagt taagccgtct tcgctgattt cccagctttc tgccaatgcc
     4381 ggttcaatat cggttgtacc cggcacaaat tccaccaagc ggttgtaaat ttgttgcgag
     4441 ctggcgttat aagataaacc gtccatcatt aaaatcgggc taaaaccggt tggcgaacgg
     4501 ctaacgcagt ttacaaaggt tttctcggtt tgcggttttg ctgctgccgc agtaggttta
     4561 ttttcgttgc ttttatcatc gcaagccgct agagctaata ccgctaaaac gcttgcacta
     4621 attttggtta acgttggaaa tttcatatta ttctccgctt aatcttagga ttaacttacg
     4681 aagattacct tttccacaat gaggagtaaa gaacaaaaag cattattttt gcaaatcaat
     4741 ccatagaatt agataccgtc aacgtcattg ccacccgtga tccaagtaaa tttgccgata
     4801 cgccacaaaa acaaactaaa gatgcacttt tggtaaaaca agcgacgagt gtggcggcgg
     4861 cattaaaagc gttgccgaat gtggatgtgc aaggaggggc ccgtgcgatt gtgcagaagc
     4921 cgtctattcg tgggttgagc gataatcgta ttgtgcaggt gattgacgga gtgcgtcaga
     4981 attttgattt ggctcaccgt ggttcttatt ttgtgccgat gtcattgatt caagaaattg
     5041 aagtaatcaa aggtccaagc agctcgttgt gggggagcgg tgccttaggt ggggtggtgg
     5101 caatgcgcac accgaatgcg ttggatttat tgaaaaataa tgacaaattc ggggcaaaaa
     5161 tccgtcaagg ttatcaaacg gcgaataatc tttccgaaac cgaagcctcg gtgtttgcag
     5221 caaatgatcg gtttgatgcc ctgctgagtg gtttctacaa taatgctgac aatttgcgta
     5281 gcggtgaagg caaggaactg aataacacgg gctataagca agccggcggt ttagtaaaat
     5341 tcggctggca aattaacgat actcatcgtt tggaattatc ccatcgtcat agccaattta
     5401 aacaaactgc accgggtaat aacgaggtag aaaatcaatt taccgataat gatgttagtg
     5461 cacaaattag tgcttggcat gcggctaatc ctggaaatcc aagtaatcca gtatatcttg
     5521 cgaaaatgtt agccttttac ggtcaattac attcaagtct tggtagtgtg agttatttaa
     5581 gtgatcaaaa aattaccgat aaaagcacct cacttaacta ctatttcaac ccaagcaatc
     5641 cttatttgag cacacaagtg acattatata gcaatagtac cactgaaaac gaacatcgaa
     5701 aaataagtgg tgtggaagat caaactaaac taagcactcg tggtgtgaat atccgtaatc
     5761 attccgaatt gtcttggttc tccttggtgt atggcgtgga ttatatgttg gataaagtag
     5821 ccactcagcg tgggagaaat aatacggacg cacaataccg tgctaatcct tatgatgcta
     5881 aatctaaaac tacaggtgct tatttaattg cacatattcc gctgtttgat gaaaaagtgg
     5941 tcttttctcc gagcgtgcgt tacgaccgtt tcgatacttc aagtgaaacg gtgaaatata
     6001 aagacagcca ttggtctcct gctgcaaaac ttacttggaa agcgacaaat tggttggatt
     6061 taaccgcgaa atataacgaa gctttcagag ccccttctat gcaagaacga tttgtgagcg
     6121 ggtctcattt tggtaccaca gtgagaggat tacctattaa taatattttc ttaactaacc
     6181 ctaatttacg cccggaaacg gcaaaaaata aggaagtaac cttcaatgta cattttgata
     6241 atgtcatctc gcaaggagat aaatttaact ttgaagcgac ttatttccgt aatgatgtga
     6301 aggatttgat taatcttcaa gtattcaaag caaatcctac aagcccaatg gaatttattc
     6361 ccactcgttc ccaatatcaa aatattgcga atgcacgttt gagtggtatt gaattacaaa
     6421 ctcgttatca aactgaacgt ttggcagttt ttgctaacta cggtagtaca aaaggtaaag
     6481 ataaagacag cggggaagcc ttgtcgaaca ttgctgcaag caaaatcggc ttcggggtca
     6541 attatgccgt ggtgcaagac aaattcacgg tgggggcaaa cgtaacccgt tatcaagcac
     6601 aacatcgtgt gccgaaaaac cacggcgtga cttaccaagg ctatacgctg accgatctac
     6661 acgccactta cgccccatta aaaggcgaat ggaaaaacct gcgtttagac gtggcggttg
     6721 aaaacctatt cgataaaaaa taccaaccgg cattcagctt aatggaaggc agcggtcgta
     6781 atgtgaaact cagtgcggct tacagcttct aatgtcttta acggaagacg gcacaagcgg
     6841 tcgttttccg gttatttttt gcaatattaa aagggagtta ctatgaaatt ttatccgtcg
     6901 ctgatcgcaa gtgcggtcag ttttgccgtt gttttgagtg gacaagcggc agtgaatgtg
     6961 ccggatgccg gctcgttaca gcgtgaatta gaccaacgtc aagctcaaca aaaagtcaaa
     7021 ccgagtggcg aatggtttaa tcaagcggca aaagcacctt ataccgcaac ggacggtcaa
     7081 gaactgacat ttccattaac tcaggtacaa gtttttgatc gaaccaacca agaaatctct
     7141 accccggaat tagccccgat tttggcaaac tatgtgggta aagcggtttc gctgaccgat
     7201 ttaagcaact tggcaaatga gatcaccgag ttttatcgta gccacaatta tctggtggca
     7261 aaagccattt taccgccaca ggaaattgaa caaggtaagg tgaaaatcct gttgtttaaa
     7321 ggcaatgtcg gtgaagtgcg gttacaaaat cagagtgctt taagcgataa atttgttgca
     7381 cgccttgcca acaccacagt gaatggttcg gaatttatcc taaaagacga attggaaaaa
     7441 tttgccctca cgatgaatga tgttcccggg gtgaataccg gcttggagct ttctgccggt
     7501 aagcaagcag gcgaggcgaa tttgctgatt aagctccaaa atgccgagcg tttctcaggc
     7561 tatgtatcgg cagataacca aggcaataaa aataccggac gttaccgttt agccacaggg
     7621 ataaaagcca ataatctgat aggttggggc gatgagttga aagtggattt gatgagttcc
     7681 aacaacgcta atttaaaaaa tgcgcgtttg gattattcga gcttgattga cggctacgct
     7741 acccgtttgg gcttaacggc gaattatctc gactacgaat taggcggtaa ctttaaagcc
     7801 ctgcaatccc aagggcatac tcacaatctt ggggcttatg tgttgcaccc gacaatccgc
     7861 ttgccggatt ttcggttaaa caccaaagtc agctttaatc atcaacgttt aaccgacaaa
     7921 caacaagcgg tcaatgtggc acaaaaacgc caagtaaaca gcttaacggt gggcattaac
     7981 gggtcgtgga attcgcttaa agacggcaca agctatttct ctctgtctgc cttgttcggc
     8041 aatttagcta accacaccaa cgagcaagcg cattatgtgg caacagattt tcagcccaaa
     8101 tcccgtttta cggtctatca atacagcctg tctcacgaac aagcgctgcc aaaatccttt
     8161 gccttcaacc ttggcgtgaa cgggcagttt gcggataaaa ccctcgacag ctcacaaaaa
     8221 atgttactgg gcggtttata cggcgtgcgt ggtcatcaag ccggtgtcgc ttcggtggat
     8281 gaggggcatt tagtgcaaac cgaactcaag cattatttac cgctcttttc caaaagcctg
     8341 ctgaccagct cggtcttcta tgattatggt tggggaaaat actataaaca cagcggattt
     8401 ttggcgcgtg gtgtaaacaa tagcgtgaaa ttacagagta tcggagcagg gctttcgctc
     8461 tctgaagcag gcagttatgc cattaacgcc agtgttgcca aaccgctggg taaaaaaatc
     8521 gagaatgcag ataaacacca attctggttc tccgccatca aaaccttcta aggagacaaa
     8581 ctatgtttaa acgtaatgca atttctctct gcattttgac cgcttgcagc ggtttagcct
     8641 atgccaatac agcagatttg ccgcaaggtg aaagggtgcg tgtcgggacg gcaacgattg
     8701 agcgcacaac cgataaaatg accgtgaatc aatccagcaa taaagtacaa attgattgga
     8761 acagctttga tattggtaag aataaagaag tggaattcaa acagccgaca gagatggcgg
     8821 tggcatataa ccgtgttacc ggcggaaacg ccagtcaaat tcaaggcaag ctgaccgcaa
     8881 acggcaaagt gtatttagcc aacccgaacg gtgtactcat caccaaagat gcggaagtga
     8941 atgtgggcgg tttgttagtg accacaaaag atttagaaaa aatcagcgaa aacggcacaa
     9001 aagataaatt cagtcgcaaa ttagcgaaag aaggcaaaat tcttaaccaa ggtaaaatta
     9061 cggccaaagg cgagcgggct tttgtggtat tggtgggcga cgatgtggct aatgaagggg
     9121 aaattaacgc caaaaattat taccacaaag aaacgaaaaa agtcaccgaa tgttatgacg
     9181 attcttggag cttggtaaaa caatgctacg attttgataa agaaatcgtg aaaagtacca
     9241 gtggagaagt gcatttatcc tccggagaaa atgtcacctt tactttaagc gaccgctata
     9301 ttgatgtggc gttagacgat aatacggtca atagcattat caaaaatgac ggggcaatca
     9361 tcgccaatgg tgacattacc ctcactgcca aagggcgaaa tcaagcctta gacagcttaa
     9421 ttatcaataa cggcgtattg caagccaata aggtgcaaaa taaaaacggt acgatcattt
     9481 tatctgccgg tgaaacccaa ttacacgaaa aaagccaaat taaaggcgag aaagtgagct
     9541 ttgccgaagc ttcttattac ggtccgacag aagacattaa agtggtttcc aaaaccggct
     9601 caaaagtgac cgcacctgtg atcgatttca aaggtaaaac cgtgaatatt caaggggaat
     9661 ttgggcgtga agacagcacc gatctctata acgatgaact acaaatcctc aaaaccacgg
     9721 tgaatattga agtcccgaat acggaagata ttcgcattgc agataccgac aacggaggta
     9781 gcggttcctt tatacaagcg ggtgctttag cctcattact tgcaaataac ggacgggtta
     9841 atgtacaggg caaaggtttt gatgtctcag gcaagttgaa tgtgaatgcc tttaaacaaa
     9901 cggattccgt gctgaaattt atcaatcagg cgggcatcaa tatccacaat gccgatattc
     9961 acgcaaacgg gcgtttattc tttgtgacaa acttgccgaa tgacaccgat ttccaatcga
    10021 atatccgcat taccgacagc aaaatcaact taggcaacgg ctcaatgggc tttggtcgca
    10081 gtagcaaacg ttgggaaatg cacgatgtgg ttcgacgtgc cgatacggaa ctgcgtaaaa
    10141 aattcaatct ggatatgaaa aacgtggaat taaaccaagt ggatgatttt gtggtagcgg
    10201 gcggtttcca acaagttaat ttggataacg tgaaagcccg tggcaaaacc aacttctatc
    10261 tggattccgg caactcccgc atgtttgtcg gtgagcagat ttatgaatat ggcgtaaccg
    10321 atattgaaaa acgcacccaa ttggcgaatt tagaccagcg tagaaaacgt tggaattcgg
    10381 ataactatga tgagaccgat ctcaactggg aatacaacca acgctttgac ggctttgcag
    10441 caaaacacaa tcaccgcgcg ccgattgccg atacggaaat caatattgcc aatagtgata
    10501 tcagcttaga taacggtttt gtgcatttaa tggcagaaaa aatcaatctg gacgacagca
    10561 aaatcaatat caccttcaac caagacaaca gcttggatga aagtacccaa atcaaccgct
    10621 taggactaaa cggcaaagtg tcgatgaaaa acagccatat taacgtagtg ggcgatgaaa
    10681 aagacggtat ctcaccggct cgccattatg ccacgatgtt cttagtgggg gagctgcttg
    10741 gtgaaaagag ttctattttc ctcaaatccc accaaggtgc agtattgaga accgacagca
    10801 atgttacaat tgccggtaaa aacagcaaag acgacctcaa aattaccgcc attaccaccg
    10861 gtaaaaaaat gggcaatgaa atcatcggac cggacggctt gccaacctcg gaagataacg
    10921 atgccaacgc cagcaacacc gcctttgcca tcggctatga acctaacgct acggctatcg
    10981 tcgaaaatgc aagcattacc gcattagcac caaacggcgg cttggcatac accggcacgt
    11041 ctaaaaccca aattgacgcg aaggataatg ctacctttac cttctttgaa aaacctcgag
    11101 ccggctatga caaattgctt ggcgacatca aaggtaaccc cgataaatta accccacgcg
    11161 atttcgcccg tttatacgac aacattaaag gcatcaaagc cagcagccta tccgaacaac
    11221 agcttggcct agcagacacc gcgaagaaaa ccagcgaaca caccttagca aacagcctag
    11281 acgtagaaaa tttggtttca gtgacagtgt gtgatgagca gaataagtgt gaggagcgta
    11341 tgttaggtaa caaaaatacc aatgctaaag ttattgtcgg agatatcaaa tttgagtagc
    11401 gttgaagaaa gtggtagaag ctcagacttg atctgataaa gcactataaa aagtgagagt
    11461 ttcccgttta gaatatgagt gtcaaaattc aatctaaaca aaaaggaaac tctcatgttt
    11521 tattacttac aatcccctca ttaaacacaa gaccggttta ttaaatttag cagaagaacc
    11581 gggcaatatc tctcaagcct gcaatgtggt gggtatgagc ctagatacat tctattgcta
    11641 tcaacaagcg gttatttttg gcaactaatt tgcaaaaaaa caataaaatc cgaccgcttg
    11701 tagagggtta atccgccctt agtgaaattt caccgatgcc gaaatagcgg aggttgtcgc
    11761 cctgttttag aatgagttcg ccctgctcgt taatgccttg cgaaatgccg tggatttctt
    11821 cggcttcggt aatgagctta accgctttat ggcggaaaag gtcgaaactt tgccagcgtt
    11881 cggcataatg agcgaagccg acaagcggat aaattttgag atttttttgc aattcatagg
    11941 ctaaacggca aaccaattcg ttgcggttga aatgatattg cgataaatct gcccacgctt
    12001 gcgtcacaat attttcatcc acttttgcca ttcctaggtt cagcccaatg ccgataacca
    12061 catctaaacc attgcgattg gcttgggttt ccaacaaaat gccccccatt tttttgcctt
    12121 ggtaataaat gtcattcggc catttaattt gaatatcttt tacattttga acagtaaggc
    12181 tttccgcaat gatcaaggct accactaaac ttaagggcgg taaatctgcc gcctgctcca
    12241 tcgggtaatg ccataaaatc gagaaataga gattttcact ctcgggcgaa taccaagttc
    12301 gacctcgtct gccacgtccg gcagtttgtt tttcagcgag gcaaaccgtg ccgttttcca
    12361 aacgttggtg atgagtgagc aaatattcat tggttgaatc aatttcatcg aacacaacgg
    12421 cttgcccgca aattaaagca ttttggattt gagcttggtt aagtttcatt atttccccac
    12481 ttagcgagtg attttttcgc tatcgacctc accgttttta ccgataaaac gcacttcagg
    12541 ctgaatttct accccaaatt tttctttcac tctctggcga acggttttgg ctagagccaa
    12601 gacatcttgc cctgttgcat tttctttatt aatcagcact aaggcttgct gggtatgtac
    12661 cgccgctccg ccaatttgta agtctttcag cccggtttgg tcaattaacc aacctgcggc
    12721 taattttacc gttccatcag cttgagggta gtgaggaatg gctgggaatt tggcttgtat
    12781 tggtgcaaat tgttcgtttg aaatcaccgg atttttaaaa aagctgccgg cattgccaaa
    12841 ttcgtcaggg tttggcaatt tggaggagcg aaccgcacag acttcatcaa aaatctgtat
    12901 tggcgtaacg gtttccggat tgagttgggc taatgatccg taacttagca ccggtttcca
    12961 cgctttggct aactttaagc ctaccgagat aatcgcaaat tcatcacggt attggtgttt
    13021 aaacacactt tcacgatagc caaattcgca ctcggatttg gatagcttaa agagttctcc
    13081 ggaacgtaaa tttaacactt ccacgaaatc acatacctgt tcaaattcaa ccccataagc
    13141 tcctatattt tgaatgggag cagagctggc gacacccgga attaacgcca gattttcaat
    13201 gccggcaata ttgtgtgcca acgtccattt aaccaattcg tgccagtttt ccccaccttg
    13261 aacgtgcaaa tagtggaaat gttcgtcttc gtggtgcgta atccctttga gtttatttac
    13321 taacaccaca ccgtcaaaat cctctaaaaa caaaacgtta gagccttgcc cgagaatcaa
    13381 gattggcagt tgagcgttaa aggctttttg ccattcgctc aatagctgtt cgactgtggt
    13441 aaattcaata atttgggtcg cttttgcagg caaatggaaa gtgtgaaaag gggttaagct
    13501 atgtttcatc atcaaattag ttagttaaga agtggaaata acaagcggtg atatttttac
    13561 aaaattttgc aattaagatt ttgggcaggc acaggatctg cccctacatt cagaattacc
    13621 ttgccccata taccacaatg gttttgccgt gagcagaaat taagccttcg tcttccaaca
    13681 tttttataat gcgtccaacg gtttcacggg aacaaccgac catttgcccg atttcttggc
    13741 gggtaatttt gatctgcata ccttccgggt gagtcattgc atccggcatt ttagctaagt
    13801 tgagcaaggt ttgagcaatg cgaccggcaa cgtctaaaaa ggcaagatta ccgacttggc
    13861 gtgaagtttg acgtaaacgg cgagccattt gtgccgataa atacattaaa atatcggggt
    13921 ttaaatggac tagttgtttg aattttttat aggaaatttc cgcaatttca caaggacctt
    13981 tagtgcggac gtaagcggta cgttgttgga ctttttcttc aaataaactt aattcaccga
    14041 caaactcacc tgtgcctaag ttagttaaga gcatctcttt ggtttcatca tctttgatgt
    14101 aaaccgacac tgagccattc acgatgtaga aaagtgattc tgcatcttca ccggcgtgaa
    14161 tgagtgtata tttcgccgga taacgatgga tatgacaatg ggttaaaaac cactccaccg
    14221 cagggtcgtg aatagagggc ggaggagtta cattttccag aggagctgtt gaaagtgtca
    14281 tatcttgcat agtatttcct ataactctta tactaggtta aatttttaga attttaacac
    14341 gaaattaaag gttagcctaa aaaagatttt ttgtctttaa tatcaaggct ttcgtgtaat
    14401 tccgaccaaa caatcaccac tttatctgcc ttaagttgtg tgagtaatcg ttctcttttt
    14461 tctgccagag aaagttccct ttccccgtag tctgtgcctt ctcgtaaaat gaaggaatct
    14521 aaaatattat gtaaggttga ttcttcgagt tcttgccaag gtataatcat aattaatcaa
    14581 ataaaggctt aataaatgcc cactgttttt caaaatgttg gtgttggtta tagctgaaat
    14641 tagaacgaac caagcgtaaa aattgccctt cgcagaaatt cactaaataa ccggctaagg
    14701 ctctttcgtc ttcaaagctc ttgcgttcac gcagtttact aagttgcaaa atattggcaa
    14761 attgtaactc caagttatca aagaattttg ccactcgagc tttgagcaac tcctcttcaa
    14821 acatcaaggc gtgtccggtc aggatgcggg taacgcccgg gtttttgcga gcaaattcta
    14881 aaatcacata taaaattgct cgcaccgaat tggcactgct gtcttggcgt ttattggcgt
    14941 gaatatagct ggtgagggtc tgctcgattt tctcgattaa cgcttcaaac atcttggttt
    15001 tacttgggaa atagcgatac aacgccccct ccgacacccc caccgcagcg gctaaacgct
    15061 cggtagtgat acgctgcatt ccctcttcag aatttaacaa gctgattaac acttccaaca
    15121 cttgttggcg gcgttccacc actgattttt tcggcatttt aattttaggt tctgtcattg
    15181 ccaatcctat tattgcttac ctgaatgccc aaaaccgcct tcgccacggt cggtctgctc
    15241 aaactcttgc acaatgttaa aacttgcctg aaccaccggc acaaagacta actgagcaat
    15301 acggtcgccc acgttcacgg taaaatcagc ttggctacgg ttccaaagcg acactttcag
    15361 ctcgccttgg taatcgctat cgatcaagcc gactaagttg cctagcacga tcccgttttt
    15421 atgccctaaa cctgatcttg gtaagatcac cgctgccaaa ttcggatcgg cgatataaat
    15481 tgaaatcccc gttgggatca acaccgtttc acccgctttt accgtcagcg gctcgttgag
    15541 catcgcccgt agatccaagc ctgccgagcc ttcggtcgca tacgcaggta atggaaattc
    15601 attgccgata cggctatcta aaa
//
LOCUS       DANLXF010000043        15615 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.043, whole genome shotgun sequence.
ACCESSION   DANLXF010000043 DANLXF010000000
VERSION     DANLXF010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 15615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15615
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.043"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            184..561
                     /locus_tag="PXD66_000808"
     CDS             184..561
                     /locus_tag="PXD66_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HDL6319131.1"
                     /translation="MEYQFTHSIHGVVAKCSMDHEAFARWLNAEITENPSQLAPIFAE
                     IEKCRAAFPNHYECVFEGREYSLYFDYDEVMAKANNLDAAFDDEEMEEGFQFYNEESI
                     AFCGLDDFEKFLKAYQHFVQTYH"
     gene            650..1237
                     /gene="yihI"
                     /locus_tag="PXD66_000809"
     CDS             650..1237
                     /gene="yihI"
                     /locus_tag="PXD66_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HDL6319132.1"
                     /translation="MSRTKKTRRITDIMPARKTDKPKQPMPKLGGGKNRKLTRYELDA
                     QAREEKRKRKHKGLPTGSRNADPAEQKKAVVKEVKDPRIGSRKKVPLMVEFVNQPEKG
                     RTIKAVPVEPIKPTLSPEQELEQLENNECLNQLLDDLEAGKTLSAEDQKFMNECLDRI
                     DELMTELGIEYEDEEGDNGDALLRQFETMDINKFR"
     gene            1251..1718
                     /locus_tag="PXD66_000810"
     CDS             1251..1718
                     /locus_tag="PXD66_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2489 domain-containing protein"
                     /protein_id="HDL6319133.1"
                     /translation="MLRIFLMVLAALILISLGGYALHLMLKVRAQKKHEQALIEQAKQ
                     AQKDRYIRILDSIEVIARAMISEQCDFSEGVLRLKPLLDVLGRKLSDYTAMWALYGVV
                     ENMPILDERKNLKRNERMKLDLEREAKEAELESDIKNECYQLLKDIEEMRKAI"
     gene            1718..3085
                     /gene="hemN"
                     /locus_tag="PXD66_000811"
     CDS             1718..3085
                     /gene="hemN"
                     /locus_tag="PXD66_000811"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDL6319134.1"
                     /translation="MSEIIWDLALIQKYNHSGPRYTSYPTALEFNENYTNEDFIRAAA
                     RYPERPLSLYVHIPFCHKLCYFCGCNKVITRHRHKVEIYLDYLEREIKTRAPLFQNRL
                     VTQIHWGGGTPTYLDEDQSARLMQMLRANFDVSDDAEISIEMDPREIELSMLDHLRAI
                     GFNRLSMGIQDFDKEVQRLVNREQDEEFIFALMKRAKELGFVSTNVDLIYGLPKQNVE
                     SFMYTLKRVVELNPDRMSVFNYAHLPSRFAAQVKIKEELLPAPETKLTILQNSIEFLG
                     KNGYKFIGMDHFAKPDDELAIAQEKGILHRNFQGYTTQEECDLLGMGVSAISLLGDTY
                     AQNRKELQQYYADVETNGIALHKGIALTQDDCIRRDVIKALICNFKLDFATIEKQYGI
                     DFKRYFAEDLAFLAPLAEDELLTMNDDSIVVSPRGRLLIRNICLCFDVYSRQLARRQQ
                     FSRII"
     gene            3159..5492
                     /gene="lptD"
                     /locus_tag="PXD66_000812"
     CDS             3159..5492
                     /gene="lptD"
                     /locus_tag="PXD66_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HDL6319135.1"
                     /translation="MKQRYSLLSLAVMAACYSNSAAADLKQQCLLGVPHFQGEVVEGD
                     QLRMPVTIEADSAVINQPRDATYTGDVTIKQGNRSIWADQVRVEQDGENARMAYLQGN
                     FNYQDNLIQATGSDATINLLSKDANLANANYQLIGRQGRGTAESGEFGNSKHTLKNAS
                     FTSCLPNDNSWQIEANEMIQHVDEEYAEMWHARFKVLGMPVFYSPYLQFPIGDRRRSG
                     LLIPSFERSSKNGWTYSQPFYWNIAPNMDSTITPTYYSRRGWQISPEFRYLTKIGQGL
                     VATEYMAKDRLSEYQPGNDDRKRYLFHWRHAMSFLTDWRFSVDYTKVSDRRYFSDFDS
                     DYGSSTDGYATQNFKLGYYQPNYNISISGKKFQTFDELDIGPYRVLPQIDFNYYKNDL
                     IKGGDFKFFAQAARFENDSKLMPKAWRFHAEPTLNFPLANRYGSLNLETKLYATYYQQ
                     TKGSESTEEIKKSVSRVLPQIKLDFKTVLESDKQLFSGYTQTLEPRVQYIYRPYKDQS
                     DIGSKAHRSVSLGYDSALLQNDYFSLFNDRRYSGLDRISSANLVTAGGTTRFFKEATG
                     EEVFNFSLGQTYYISPSKIDDTSDNSTKGHTTSWAAESNWKFHKNWNWHGSYQYDTRL
                     NQTALANMSLQYKPQSDKLIQLNYRYASKDYINQNLNTNKYGQSIKQVGGVLGWELTD
                     KVSVMTSYYHDLALKKPVEGQISLNYNTCCWAANVYVARRLVSTPVGSSDTINDFYYD
                     NKFGINFELRFGTNYSNGVTRMLKRGIIPYTEPYSIN"
     gene            complement(5634..8273)
                     /gene="ppc"
                     /locus_tag="PXD66_000813"
     CDS             complement(5634..8273)
                     /gene="ppc"
                     /locus_tag="PXD66_000813"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HDL6319136.1"
                     /translation="MNQQYSTIQHNIHMLGEFLGETIRDAQGNYILELIESIRVLSRN
                     SRAGDENAREQLLDKLANISNDDVLPVARAFSHFLNLTNIAEQHQTVSRHHIDESLGS
                     RSLDSLFLRLKSQNIPVEKVIKTVESLMIDLVLTAHPTEVTRRSLGHKHVEINKCLNL
                     LEHDDLTEAESYKIKRRLMQLIALAWHTNEIRTQRPTPVDEAKGGMAVIENSLWKAVP
                     EFCRQLNFYLEKHFGVQYPVNLAPVRFSSWMGGDRDGNPFVTAETTRRVLRMNRWKAT
                     ELFLQDIKNLAEELSITQCTPAFRAKYGNHLEPYRVLLKVLRQKLINTEIYYAELLEG
                     KISTIDESDILTSDQQLWEPLFDCYESLQACGMRIIANGALLDCLRRINCFGLGLSRL
                     DIRQESTRHEMAIAEITRYIGLGDYAHWTEDDKQAFLVRELSSRRPLIPTNWTPSPET
                     KEVLDTCKIVAEQPEGVISAYVISMAREASDVLSVHLLLKEAGCKFTIPVAPLFETLG
                     DLDHSEKVMTDLFNIGWYRGVINNYQMIMIGYSDSAKDAGMLAAGWAQYRAQEALVNL
                     CEKYNIELTLFHGRGGTIGRGGAPAHAALLSQPPRSLKNGLRVTEQGEMIRFKLGLPA
                     VAVESLELYASAILEANIFPPPEPKQQWRDVLAKASDISCQIYRDMVRGELDFVPYFR
                     SATPEQELSRLPLGSRPAKRNPKGGVESLRAIPWIFAWMQNRLMLPAWLGAGQALQQL
                     IDEGNETLLKEMCNEWPFFSTRIGMLEMVFSKADIWLAEYYDQRLVEPKLHRLGNMLR
                     TQLQADLKTVLALAHDGQLMADLPWIAESIALRNIYTDPLNLLQVELLDRIRKQGENA
                     DPVLEQALMITITGIAAGMRNTG"
     gene            8398..9366
                     /locus_tag="PXD66_000814"
     CDS             8398..9366
                     /locus_tag="PXD66_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family transporter"
                     /protein_id="HDL6319137.1"
                     /translation="MCLKLLFGFDNLPVVYVLFTVIQVGLSINGFKGIKWLENVSCVF
                     LIGILAYMLYVVNTKFSVEIGERFANIEGTWGMPFWAATTAFLGIYSTMILNASDYSR
                     HVKKGTTPTATGVIYVLSILPVTLFMGLIGLLVTAATGNSDPIEVFATAMDNKFLTVI
                     TLLFIAFAQVTTNVLNNIVPPSYILMDSFKMKWSHATILVGILSICVMPWKLVTAESA
                     DGLSLFIKIYSAFLGPIFAVMVVDYYILRKRTLNVNDLYNKEGIFKGINWAAIIAIAI
                     GSFCSLIVVDLSWYVSLIPSGLSYYILMRVLKSSEAFRKGTVLEHQ"
     gene            complement(9501..10556)
                     /locus_tag="PXD66_000815"
     CDS             complement(9501..10556)
                     /locus_tag="PXD66_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL6319138.1"
                     /translation="MGRPRKQQNRYLPENLRPKTVTRKSGKTVIYWLYRTHGKTEISL
                     GTNRNEAFIKAAQLNIERESQTARITFGLVAKRYRDEVIPQKKASTAQTDLCKIKNLL
                     LFFNDAPLEEITPQHIREYMIWRKDTPGSANNEFSMFSHIWQYAREWGYTNLPSPNIG
                     MKRYPIRKREIYVENNIFQMVYEVADQDMRDLMDLAYITGQRPVDLVNIRKEHIFDGY
                     LHIVQQKTQAKLRIEIVGKLADIIQDRLEKATGTHLFSTKKGEKLTARKLGKRFTYIR
                     EQAVKNNPEYANEIMAFQFRDLRAKSGTDKALSLGEEAARQQLGHTSVQMTKTYIRKA
                     PIVTPIKESVPSSKHQS"
     gene            complement(10516..10791)
                     /locus_tag="PXD66_000816"
     CDS             complement(10516..10791)
                     /locus_tag="PXD66_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HDL6319139.1"
                     /translation="MEQPTEFLTKDELADLTGYRQKKRQIEWLRERHYIIQDENKYKP
                     LVLYKDVYGHPRQQYPEAQSTGKWKSPAYLMGVKNGKTTQTTESLLT"
     gene            complement(10881..11387)
                     /locus_tag="PXD66_000817"
     CDS             complement(10881..11387)
                     /locus_tag="PXD66_000817"
                     /inference="COORDINATES: protein motif:HMM:NF024784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL6319140.1"
                     /translation="MRILPRNENYAATENGEIFSFLSNKILKPRPLKNGYLRVQLVDE
                     NGVKDYLIHRLVCETFHGYPDLHVNHKDRDKTNNRPENLEWCTRSENMLHCSRLGGFS
                     KQSERMKKMNIESCSVPVAAITLDGKLFKKYTSMTSAGKDGFSHSKISLCIQGKRKTH
                     KGYIWQKL"
     gene            complement(11365..11961)
                     /locus_tag="PXD66_000818"
     CDS             complement(11365..11961)
                     /locus_tag="PXD66_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017807451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDL6319141.1"
                     /translation="MPLSITANPTKAVQLKHQGEALSGGSETLVVHGTQDPIISQNKA
                     HCLGRNNGQENVLFEVKGAEAVRISEIQDKTPTLKARMGTGGNNIPCIALAGNTIGRQ
                     PQNDGNGNVFDESGVSYMLTRTDVHAVSAGTTIRKLTPTECERLQGFPDGWTKITYRG
                     KSAEECPDSPRYKAIGNSMCTNVMKWIGERLSAYLTSQ"
     gene            complement(11882..13201)
                     /locus_tag="PXD66_000819"
     CDS             complement(11882..13201)
                     /locus_tag="PXD66_000819"
                     /inference="COORDINATES: protein motif:HMM:NF024795.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hint domain-containing protein"
                     /protein_id="HDL6319142.1"
                     /translation="MNNIIKFTYGSICSGIEAASVAWHDIGTPLWFSEIEPFPCAVLA
                     HRFPDVPNLGDMTALPEKILNRKIPAPDVLVGGTPCFTAGHMVLTDKGYMPIETLSVG
                     DLVVTHKGQLKPILRVGSEIKPVGKLTAVGLPESIVCTPEHPFYAQKWQTINTKRNGK
                     YHRKTIISKPEWIEAHKLEGYQWVSLTDFSVMPYGGFHSKLTDDEVLLIAGYYLGDGW
                     IRRWKGKNKKAVVISVNKEKYRKFSLSFAKLPHHITTENNSVIKITICDTELADFLYS
                     QFGEKAGGKTIPAWCLSHSFRSKIFEGYMITDGSFRNGVYTANSISKSLAYGIAALSQ
                     TLGYISSVSKVTVAPTKEIQGRIVNQNDYYQMRAFLQSTSRKSRLDENRLLRSVQSFE
                     ITGNEVVYNIEVADDNSYILNNAVVHNCQSDKGGTVKASGGGFEWRE"
     gene            complement(13198..14103)
                     /locus_tag="PXD66_000820"
     CDS             complement(13198..14103)
                     /locus_tag="PXD66_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015701527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDL6319143.1"
                     /translation="MQFQEHNNRKKADKFAEYITGEALRRYVAEKVKQYARNNVSVFD
                     GAAGSGQLEQFVRPTSFTAVEVQSESCDVLRHNFPTAQIYNQSFFLYPNGEPCDCVIM
                     NPPFSLKFKDLSQAEQAQIQSEFPWKKSGVVDDIFVLKGLANSHQWGFFILFPGVGYR
                     QTEKRFREEVGNQLVELNRIQNAFEDTPIEVLFLVVDKTKTTDDCSRELIDCAGGQIQ
                     QICADKWKIRPDHWESIQPPPPPQEEIKPLELEAFAREQAIERIVGEVRLSKLIAEFF
                     EPELRRTFNAFIDDICVAVQAEKIV"
     gene            complement(14337..14993)
                     /locus_tag="PXD66_000821"
     CDS             complement(14337..14993)
                     /locus_tag="PXD66_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HDL6319144.1"
                     /translation="MTNSLITVFDGTIQNTQIQLCNARELHSFVESKQQYSHWISDRI
                     TDYGFVQDEDYIIVTERTAGRPRKEYHITLDMGKELAMVERNEKGRQIRKYFIECERK
                     MKELLQNNQQNLPLALPEKKYTFEFTEDELQSLVWAWFAFVRGIHTFRYIYPMFQKLG
                     SNIAPEIYGQGFEYSHTAQSAHKILERITKEFDYDPMTNWRVLKHVRGFDPAFKKPTF
                     "
     gene            15397..15585
                     /locus_tag="PXD66_000822"
     CDS             15397..15585
                     /locus_tag="PXD66_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319145.1"
                     /translation="MKELFKQWLINQDSPFINSCGVECILSKVDDWLNIINANEEETE
                     TLIEWRNAFLQDVSVFIA"
BASE COUNT         4469 a         3061 c         3308 g         4777 t
ORIGIN      
        1 tggcatagta gttccttttt gatgtatcaa tactaaaaat aacctgtatt attctagcag
       61 atttctgggt aatcgaggag ttgataacat ttaaaaatta gcgttttgca aaattttcca
      121 aaaaaagacc gcttgttagg ctataatagc caaccaaaca gaccaattca aaggagaatc
      181 ggtatggaat atcaatttac ccatagtatt cacggggtgg tggcaaaatg ttcgatggat
      241 cacgaggcgt ttgctcgttg gttgaatgcc gaaataacgg aaaatcctag ccagttagcc
      301 cctattttcg ctgaaataga gaagtgtcga gccgcatttc cgaatcatta tgaatgtgtg
      361 tttgaagggc gggagtatag tctctatttt gattatgatg aagtgatggc aaaagcaaac
      421 aatttagatg ctgcttttga tgatgaggaa atggaagaag gctttcaatt ttacaatgaa
      481 gagagcatcg cattttgtgg cttagatgac tttgagaaat ttttgaaagc ctaccaacat
      541 tttgtgcaaa cttatcattg attgaaatga ttaattgaaa ccaaagctat taagggaatc
      601 taattccttg tagaatagcc gtttatagca attttaagat attaaattta tgagtcgtac
      661 taaaaaaaca cgtcgtatta ccgacattat gccggcaaga aagaccgata aacctaaaca
      721 gcccatgcct aaattaggtg gggggaaaaa tcgcaaactg acccgttatg agttagatgc
      781 tcaagcccgt gaggaaaaac gtaagcgtaa acataaaggc ttgccgacag gctcacgcaa
      841 tgctgatccg gcagagcaga aaaaagcagt ggtgaaagag gtgaaagatc cgcgtatcgg
      901 tagccgtaaa aaagtgccgt taatggtgga atttgtgaac cagccggaaa aagggcggac
      961 gattaaggct gtgccggttg agccaatcaa accaacgctt tcaccggaac aagagctgga
     1021 gcagttagaa aataacgaat gtttaaatca gttgttagat gatttggagg cgggcaaaac
     1081 cttatccgca gaagatcaga aatttatgaa tgaatgttta gatcgcattg atgaattaat
     1141 gactgagctt ggcatcgaat atgaagatga agagggcgat aatggcgatg cgttattacg
     1201 ccaatttgaa acgatggata ttaataaatt ccgctaggta gccgattatg atgctaagaa
     1261 tattcctgat ggtgcttgcc gctttgattt taatttcgct gggcgggtat gcactgcact
     1321 taatgctgaa agtgcgagct cagaaaaagc acgaacaagc actgattgaa caggcaaagc
     1381 aagcacaaaa agatcgttat attcggattt tagacagcat tgaagttatt gctcgggcaa
     1441 tgatttcaga acagtgcgat ttttcggaag gcgttttacg tttaaaaccg ctattagatg
     1501 ttctcggcag aaaattaagc gactatacgg cgatgtgggc gttgtatggc gtggtagaaa
     1561 atatgccgat tttagatgaa cgcaaaaatt taaaacgtaa cgaaagaatg aagttggatt
     1621 tagagcggga agcaaaagaa gcggagcttg aatcagacat caaaaatgag tgttaccaat
     1681 tattaaaaga tattgaagaa atgagaaagg caatctaatg tcagaaatta tttgggattt
     1741 agcccttatt caaaaatata accattcagg gcctcgttat acctcttacc caacagcgtt
     1801 ggagtttaac gaaaattaca ccaatgaaga ttttattcgt gctgcagctc gttaccctga
     1861 gcgtccgctt tcgctttatg tgcatattcc gttttgccat aaactgtgct atttctgtgg
     1921 ctgtaataaa gtgattactc gtcatcgcca taaagttgaa atttatttag attatttaga
     1981 gcgtgaaatt aaaactcgcg cacctctgtt tcaaaatcgt ttagtgaccc aaatacactg
     2041 gggtggcggt acgccgactt atttagacga agatcaatcg gcacgtttaa tgcaaatgct
     2101 acgagccaat tttgatgtga gcgatgatgc ggaaatcagc attgaaatgg atccgcgtga
     2161 aatcgaatta agtatgttgg atcacctgcg tgctatcggt tttaaccgtc tgagtatggg
     2221 tattcaggat ttcgataaag aagtgcaacg tttggtgaat cgtgagcaag atgaagagtt
     2281 tatttttgcc ttgatgaaac gggcaaaaga acttggtttt gtttcaacta atgtggattt
     2341 gatttacggc ttgccaaaac agaatgtgga aagctttatg tacaccttaa aacgtgtggt
     2401 agagctaaac cctgatcgta tgagcgtatt taactatgcc cacttgccaa gccgttttgc
     2461 tgcacaagtg aaaattaaag aagagttgtt gccggctccg gaaaccaaat tgacgatttt
     2521 gcaaaattcc atcgaatttt taggcaaaaa cggttataag tttatcggta tggatcactt
     2581 tgctaaacct gatgatgagt tagctattgc acaagaaaaa ggcattttac accgtaactt
     2641 ccaaggctat accactcaag aagagtgtga tttattggga atgggcgtat cggcaatcag
     2701 cttactcggc gatacttacg cccaaaaccg caaagaatta caacaatact atgctgatgt
     2761 tgaaaccaac ggtattgcgt tgcataaagg cattgcgtta acccaagatg attgcattcg
     2821 ccgtgatgtg ataaaagcct tgatttgtaa tttcaaatta gattttgcca ccattgaaaa
     2881 gcaatatggt attgatttta aacgttattt tgccgaagat ttagctttct tagcgccgtt
     2941 ggcggaagat gaattattaa cgatgaatga cgacagcatt gtggtttccc cacgagggcg
     3001 tttattgatc cgtaatattt gcttatgctt tgatgtctat tcaagacaat tagcgagaag
     3061 acagcagttt tcccgtatta tttaatgagc aagcggtcga ttttggtaaa aattttgcaa
     3121 aaacagaccg cttgttcccg gcttataaag gaaaaatgat gaaacagcgt tatagcttac
     3181 tttctcttgc tgtgatggca gcttgttata gcaacagtgc cgcagctgat ttaaagcaac
     3241 aatgtttact tggcgtgccg cattttcagg gcgaagtggt agagggcgat caacttagaa
     3301 tgcctgttac cattgaggct gacagtgcgg tcattaacca gcctcgtgat gccacctaca
     3361 ccggtgatgt aacgatcaaa caaggtaacc gttcgatttg ggcggatcag gtgcgggttg
     3421 agcaagatgg cgaaaatgcc cgaatggcgt atttgcaggg taattttaac taccaagata
     3481 atttgattca ggcaaccggt tcagatgcga ccatcaatct gctttccaaa gatgcgaatt
     3541 tagctaatgc taattaccaa ttaattggca gacaagggcg tggcacggca gaatccggtg
     3601 aattcggcaa cagcaaacac acgctgaaaa atgccagttt cacttcgtgc ttaccgaatg
     3661 ataattcgtg gcaaattgaa gcgaatgaaa tgattcagca cgttgatgaa gaatatgcgg
     3721 aaatgtggca cgcccgtttt aaagtgctgg gaatgccggt attttattcg ccttacttac
     3781 aatttccgat tggcgaccgc cgccgtagtg ggttattaat tccaagtttt gagcgttcaa
     3841 gcaaaaacgg ctggacatat tcgcaaccgt tttattggaa tatcgcaccg aatatggact
     3901 cgaccatcac tccaacttac tattcacgcc gtggttggca aatcagcccg gaatttcgtt
     3961 atttaacgaa aatcgggcaa ggcttagtgg caaccgaata tatggcgaaa gatcgtttaa
     4021 gcgagtacca accgggaaat gatgatcgta aacgctattt attccactgg cgtcacgcaa
     4081 tgagtttttt aaccgattgg cgtttctcgg tcgattacac caaagtgagc gacagacgtt
     4141 atttctctga ttttgattcg gattatggta gcagtactga tggctatgcg acgcagaatt
     4201 ttaaattagg ctactatcag ccgaattata atatctcaat ttcaggtaaa aaattccaaa
     4261 ccttcgatga gttggatatc gggccttatc gcgttctgcc acaaattgat tttaactatt
     4321 ataaaaacga tctaattaaa ggcggagatt tcaaattctt tgcccaagca gcccgttttg
     4381 aaaatgatag taaattaatg ccaaaagcgt ggcgtttcca cgctgagcca acactgaatt
     4441 tcccattagc gaaccgctat ggtagcttaa atttagaaac caagctctat gcaacctact
     4501 atcaacaaac gaaaggtagt gagagtacgg aagaaattaa gaaaagtgta agtcgtgtac
     4561 tgccacagat taagctcgat tttaaaaccg tgttggaatc cgataaacag cttttctctg
     4621 gctatactca aacgcttgag cctcgggtgc aatatattta tcgcccatat aaggatcaaa
     4681 gcgatattgg ctcgaaagca catcgaagtg tgagcttagg ctatgactct gcattattgc
     4741 aaaacgacta tttctcactg tttaatgaca gacgttatag cggtttagat cgtatttcgt
     4801 cagcaaattt agtcaccgca ggtggcacaa cccgtttctt taaagaggca accggtgaag
     4861 aggtgtttaa tttcagctta ggacaaactt attatataag cccgtctaaa attgatgata
     4921 cctcagataa cagcactaaa ggtcatacga cttcatgggc agcggaatct aactggaaat
     4981 tccataagaa ttggaattgg catggtagct atcaatatga tactcgttta aaccaaacgg
     5041 cgttagcgaa tatgtcattg caatacaaac cacagtcaga caaattgatt cagcttaatt
     5101 accgctatgc aagtaaagat tacatcaatc aaaacttgaa cacgaataag tatgggcaat
     5161 ccattaaaca agtgggcggt gtgctaggtt gggaattaac cgataaagtg tcggtaatga
     5221 ccagttatta ccacgacctt gcattgaaaa aaccggtgga agggcaaatt tcgcttaact
     5281 acaacacttg ctgttgggcg gcaaatgtgt atgtggctcg tcgcttagtt tcaacgccgg
     5341 taggttcatc tgatacgatc aatgatttct actatgacaa taaattcggc attaactttg
     5401 agctacgctt tggcacaaac tacagcaatg gtgtaacccg tatgctaaaa cgaggtatta
     5461 ttccttacac cgagccgtat agcattaatt aacaaagtaa aaacaaataa gtaaaaatac
     5521 aaatcggggc gaaaaatatt tgccccgatt tttttatatt tggctgataa gcggttaaat
     5581 tctgttggaa ttttgcaaat cttccgctaa attcaaccgc ttgccatcga aacttaacct
     5641 gtatttcgca tacctgcggc aatacctgtg atggtgatca tcaatgcttg ctctagtaca
     5701 ggatcagcat tttcaccttg tttgcgaatc ctatctaaca attctacctg aagtagattg
     5761 agcggatcgg tataaatatt gcgtagtgca atcgattctg cgatccaagg taaatcggcc
     5821 attaattgtc catcgtgagc cagtgcaagt acggttttaa gatcagcctg tagttgcgta
     5881 cgcaacatat tgcctaaacg gtgtagtttt ggctcgacta ggcgttggtc gtaatattcc
     5941 gccagccaaa tatcagcttt actaaatacc atttccagca ttccgatacg ggtcgagaaa
     6001 aacggccatt cgttacacat ttcttttagt aaggtttcat taccttcatc aatcaactgt
     6061 tgcaatgctt gccctgcacc taaccaagca ggtagcatta agcggttttg catccaagcg
     6121 aaaatccaag gaattgcacg caaactctcc actccgcctt ttggattacg ttttgcaggg
     6181 cgtgagccaa gcggcagacg ggaaagttct tgctctggtg tagcagagcg gaaatacggt
     6241 acgaagtcta gctcgccacg caccatatca cggtaaattt ggcaagaaat atcggaggct
     6301 tttgctagca catctcgcca ttgctgcttt ggctccggtg gtgggaaaat attcgcttct
     6361 aaaatcgcac tggcatagag ttctaaactt tcaactgcca cggcaggtaa gcctaattta
     6421 aagcggatca tttctccttg ttcggtcact cgtaagccat ttttgagtga gcgaggtggc
     6481 tgagaaagta aggctgcgtg agccggtgcg ccaccacgcc cgatagtacc accacgaccg
     6541 tggaataagg ttaattcaat attatatttt tcacataaat taaccagagc ttcttgcgcc
     6601 cgatattgcg cccagccggc agcaagcatt ccggcatctt tggcggaatc agagtagccg
     6661 atcataatca tttggtaatt gttgatgacg ccacgatacc agccgatatt aaataaatcg
     6721 gtcatcactt tttcactatg atctaaatcg cctagcgttt caaataatgg ggcaaccgga
     6781 atagtaaatt tgcaccccgc ctctttgagt aaaaggtgaa ccgagagtac atcagaggcc
     6841 tctcttgcca ttgaaatgac ataggctgaa atgacacctt caggttgctc ggcaacaatt
     6901 ttacaagtgt ctaacacttc tttagtttca gggcttggag tccagttggt tggaatcaat
     6961 gggcggcgag aactgagttc acgcactaag aatgcctgtt tatcatcttc cgtccagtga
     7021 gcataatcgc ctaagccgat atagcgggta atttctgcaa tcgccatttc gtggcgggta
     7081 ctttcttggc gaatatctaa gcgtgataaa cctaaaccaa aacagtttat acgacgtaag
     7141 caatctagta atgcaccatt tgcaataatt cgcataccac aggcttgtaa tgactcatag
     7201 caatcaaaaa gcggttccca caattgctga tctgaagtta aaatgtcact ttcatcaata
     7261 gttgaaattt taccttccaa taattccgca tagtaaattt cggtattaat taatttctgg
     7321 cgtaatactt ttagtaatac gcgatatggc tctaaatggt tgccatattt agcacgaaat
     7381 gcaggggtac actgtgtaat cgataattct tcagcaagat ttttaatatc ttgtaagaac
     7441 aattccgtgg ctttccaacg gttcattctt aatacacggc gagttgtttc tgccgtgacg
     7501 aacgggttac cgtcacggtc tccgcccatc caagaagaaa aacggacagg ggctaaattt
     7561 accggatatt gcacaccaaa gtgtttttct aaatagaagt taagctgacg gcaaaattcc
     7621 ggcacggctt tccataaact attttcaatg actgccatac cgcctttggc ttcatcaaca
     7681 ggggttgggc gttgggttct gatttcatta gtatgccaag caagggcaat cagttgcatt
     7741 aaacggcgct tgattttata gctttctgcc tcagttaaat catcatgttc caacaaattc
     7801 aaacatttat tgatttcaac gtgtttatgc cccagcgaac ggcgggttac ttccgttggg
     7861 tgagctgtta gcactaaatc aatcattagg ctttccacgg ttttaatcac tttttcaacg
     7921 gggatatttt gtgattttag acgaaggaac agagagtcta atgagcgact accaagtgat
     7981 tcatcaatat gatggcgaga aacagtttga tgttgttcag caatatttgt tagatttaga
     8041 aaatggctaa atgcacgtgc aacgggtaag acatcatcgt tagaaatatt ggctaattta
     8101 tctaatagct gttcacgggc gttttcatcg ccggcacgag aattgcgaga taacacacga
     8161 atgctttcga tgagttctag aatgtaattt ccttgcgcat cacgaatcgt ttctcccaaa
     8221 aattcaccta acatatggat attatgctga attgttgaat attgctggtt cataaaaaat
     8281 tcctatggta aacataaatt aaattaggtc taaatatgcc gaaaatcgct gaaaatagca
     8341 aatagattta atttaatttt tgaattaaat gataaaacgc aaaaaatagc tgtcaatatg
     8401 tgtttaaaac tgttgttcgg ttttgataac ttgcctgttg tttatgtgct attcactgtt
     8461 attcaagttg gcttatccat taatggtttt aaaggtatta aatggttgga aaatgtttct
     8521 tgtgtgttcc tcatcggtat tcttgcttat atgctttatg ttgtgaatac aaaattctct
     8581 gtagaaatag gcgagcgctt tgccaatatt gaaggcactt ggggaatgcc attctgggct
     8641 gcaactacag cattcttggg tatttattca acaatgattt taaatgcttc cgattattct
     8701 cgccacgtta aaaaaggaac aaccccaaca gcaacaggtg tgatttatgt attatcaatc
     8761 ctccctgtaa ccttatttat gggtttaata gggttgttag ttacggcagc tacaggcaat
     8821 agtgacccaa ttgaagtatt tgcaactgca atggataata aatttttaac agtaataacg
     8881 ttattattca ttgcttttgc acaggttacg acaaacgtgt tgaataatat tgttccgcca
     8941 tcttacattt taatggattc atttaaaatg aaatggtcgc atgccaccat tttagtgggc
     9001 attctttcta tatgtgtaat gccttggaaa ttggtaactg ctgaatcagc agatggatta
     9061 tctttgttta ttaaaattta ctcggcattc ttaggcccta tttttgcggt aatggtcgtc
     9121 gattactaca tcttaagaaa acgtacttta aacgtaaatg acttatataa taaagaaggt
     9181 attttcaaag ggattaactg ggcagcaatt attgcgattg ctatcggttc gttttgctca
     9241 ctgattgtgg tggatctctc ttggtatgtc agtttaattc catcaggctt gagctactat
     9301 attttgatga gggtgttaaa aagttccgaa gctttccgta aagggacagt gctagaacat
     9361 caataagatg acaagcggta aaatttatta aaaatattat cagtaataac gagtaacaaa
     9421 aattacttat ctttcaatct attataagtg atttttatgt aaacgttccg aaaaaatgca
     9481 aaatgttccg aaactgtctt ttaagactgg tgttttgagg agggaacact ttctttgata
     9541 ggggttacta ttggggcttt tcgaatgtat gttttggtca tttgaacgct agtatgacct
     9601 agttgttggc gagcggcttc ttcacctaga gaaagggctt tatctgttcc tgatttagct
     9661 cttagatccc gaaattggaa tgccattatt tcatttgcgt attccgggtt attcttgacc
     9721 gcttgttccc gaatataagt gaacctttta cctagttttc tagcggttag cttttcccct
     9781 ttcttagtgc tgaataagtg tgttccagta gctttctcca gcctatcttg gataatatca
     9841 gccagttttc ccactatctc aatacgtagt ttggcttgtg ttttttgctg aacaatatgg
     9901 agataaccat caaaaatgtg ttctttccgg atattgacca aatcgacagg acgttgtccg
     9961 gtgatatagg cgagatccat taagtctctc atatcctgat ctgccacttc atataccatc
    10021 tgaaatatat tgttctcaac gtaaatttca cgctttctga tagggtagcg tttcattccg
    10081 atattagggc ttggtaagtt tgtataaccc cactctcgag cgtattgcca aatatgactg
    10141 aacattgaaa attcgttatt agcagatcca ggtgtgtctt ttcgccaaat catatactct
    10201 cggatatgtt gaggggtgat ttcttcgaga ggagcgtcat taaagaatag cagtagattt
    10261 tttatcttgc acaaatctgt ttgtgctgtg ctcgcttttt tctgaggtat aacttcatct
    10321 cgataacgtt tggctacaag cccgaaggta attctggcag tttggctttc tctttctata
    10381 ttgagttgag cagccttaat aaaagcctca tttctatttg tgcctagaga gatttccgtt
    10441 tttccgtgcg tgcgatatag ccaataaatg acagttttgc ctgattttcg tgtaactgtt
    10501 tttggacgta aattctcagg taagtagcga ttctgttgtt tgcgtggtct tcccattttt
    10561 cactcccatt aaataagcag gtgatttcca tttcccagtg gattgagctt ctggatattg
    10621 ttggcggggg tgcccataga catctttgta tagcactaat ggcttatact tgttttcatc
    10681 ttgaattata tagtgacgct ccctcaacca ttcgatttgt cttttctttt gacgatagcc
    10741 tgttaaatca gcaagttcgt ctttcgttaa aaattcagta ggttgttcca tattctctcc
    10801 aaataacccc ctcatttaga gggattgagt taaataatcg ttaagccttt gtcctatcca
    10861 ccgcatacaa gggacggcca ttataacttt tgccatatgt aacccttgtg cgttttgcgt
    10921 ttaccctgaa tgcaaaggct gatttttgaa tgtgagaaac catctttacc tgcacttgtc
    10981 attgaggtgt attttttgaa taatttcccg tccagagtta ttgcggcaac aggaactgag
    11041 caactctcaa tattcatttt tttcatcctc tcggactgct tgctaaaacc tccaagtctt
    11101 gagcaatgca acatattttc tgagcgtgta caccactcta aattttcagg tcggttattt
    11161 gttttgtctc tgtccttatg atttacatgt agatctggat aaccgtgaaa agtttcgcat
    11221 accagtctat ggattaaata gtcttttact ccattctcat ctacaagctg aactcttaaa
    11281 taaccattct ttaaaggtct aggttttaag attttgttgc tcaagaaact aaaaatttct
    11341 ccattctcgg tagctgcata gttttcattg cgaggtaaga tacgcactta atctctctcc
    11401 tatccatttc attacatttg tacacatcga atttcctatc gccttatatc gtgggctatc
    11461 agggcattct tcggcagatt tgccacggta tgtgattttc gtccagccgt ctggaaagcc
    11521 ttgtagtctc tcgcattcgg tgggagtaag ttttcgaata gttgttccag cactgacagc
    11581 gtgaacatca gttcgggtaa gcatgtaact tacaccactc tcatcaaata cgttgccgtt
    11641 tccgtcgttt tgtggctgtc tgccgatagt attaccggca agggcaatac aagggatatt
    11701 atttccaccc gtccccattc ttgctttcag tgttggcgtt ttgtcctgaa tttcgctaat
    11761 tcgtacagct tcagcacctt ttacttcaaa taagacgttc tcttgcccgt tgtttcgacc
    11821 taaacaatga gctttgtttt ggctgataat tggatcttgc gtaccgtgaa caactagcgt
    11881 ctcactcccg ccactcaaag cctccccctg atgctttaac tgtaccgcct ttgtcggatt
    11941 ggcagttatg gacaacggca ttatttaaga tgtagctgtt gtcatctgcc acctcaatgt
    12001 tataaacaac ttcatttcca gttatctcaa aggattgcac gcttctcaat aatctatttt
    12061 catcaaggcg actttttctt gatgtcgatt ggaggaacgc tcgcatttga taatagtcat
    12121 tctgattgac aattcttcct tggatttctt ttgtcggagc aacagttact ttggaaacag
    12181 atgagatata tcctaaggtt tgggataatg cagcaatgcc gtaagcaagg gatttcgaaa
    12241 tgctatttgc ggtataaact ccgtttctaa aagagccatc ggtaatcata tacccttcaa
    12301 aaattttact tctgaaagaa tgagataaac accaagccgg tatagttttt ccgccggctt
    12361 tctcaccaaa ttgcgaataa agaaaatcag caagttcagt gtcacaaata gtgattttaa
    12421 ttaccgaatt attttcggta gtaatatgat gaggaagttt ggcaaaagaa agagaaaact
    12481 tccgatattt ctctttattt acagaaataa caacagcttt tttatttttg cctttccatc
    12541 ttcttatcca gccatcacca agataataac cggcaattag aaggacttcg tcatcagtca
    12601 gtttgctatg aaaaccacca tagggcataa ctgaaaaatc agttaatgat acccattgat
    12661 agccctctag cttatgagcc tcaatccact caggtttgga aatgatagtt ttcctatggt
    12721 attttccatt ccttttggtg tttatcgttt gccatttttg agcataaaaa ggatgttcag
    12781 gggtgcaaac gattgattca ggtaagccaa cagccgttaa ttttccaact ggttttattt
    12841 cactgccaac acgcaaaata ggttttagtt gtcctttatg agtaacaact aaatctccga
    12901 cagaaagtgt ttcgattggc atatatcctt tatccgttaa aaccatatgc ccagcggtaa
    12961 agcaaggcgt acccccaacc aacacatcag gagcaggaat tttacgattg aggatttttt
    13021 caggtagggc ggtcatatcg cccaagttcg gcacatcggg gaaacggtga gcaagcactg
    13081 cacacggaaa cggctcaatc tcgctgaacc ataacggcgt accgatgtcg tgccacgcta
    13141 cacttgctgc ctcaatgccg gagcagatag atccataggt gaattttatt atgttgttca
    13201 tacaatcttc tccgcctgca ccgcaacgca aatatcatca ataaatgcgt taaaagtgcg
    13261 gcgtaattct ggttcaaaaa attctgcgat cagcttggat aagcgaacct cgccgacaat
    13321 tcgttcaatc gcttgctctc gggcaaacgc ctctaattca agcggtttta tctcttcttg
    13381 tggcggtggt ggcggttgga tactttccca atgatcaggt ctaattttcc atttatcagc
    13441 acaaatttgt tgaatttgcc caccagcaca atcgattaac tcccgagagc aatcatcggt
    13501 cgttttggtt ttgtctacca ccaagaatag gacttcaatc ggggtatcct caaaggcgtt
    13561 ttggatgcgg ttgagttcta caagttgatt gccaacttct tctcgaaagc gtttttctgt
    13621 ttggcggtag ccaacgccgg gaaagagaat gaaaaatccc cattggtgac tatttgccag
    13681 tccttttagg acgaaaatat catcaaccac cccagatttt ttccacggaa attcggattg
    13741 aatttgtgct tgttcggctt ggcttaaatc cttaaattta agggaaaatg gcggattcat
    13801 aatcacgcaa tcgcacggtt cgccgttagg atagagaaag aagctctgat tatagatttg
    13861 agccgtaggg aaattgtgcc gtaacacatc gcagctttcg gattggacct ctaccgcagt
    13921 aaaagaggtt gggcggacaa attgctccaa ctgcccagaa cctgccgccc cgtcaaaaac
    13981 agaaacatta tttctggcat actgcttgac cttttcagca acataacggc gtagagcttc
    14041 acctgtgatg tattcagcga atttgtcggc ttttttgcgg ttattgtgtt cttggaattg
    14101 catttttcct ccaaaaaata accgcttgcg gaacaagcgg ttgggttaat tagcgttgag
    14161 ctgtacaaat tttgccatcg cagtcgttgc gtaactctag atggtcagca gcaactgcaa
    14221 aggcggttaa aaagagtatg atttttgcgt atttcatcgt ctaatcctca aaatttgggt
    14281 gcaggaaacc gccgcttgat gtttcgcaag cggtcgggtt ttataatttt ttgtgattag
    14341 aacgttggct ttttaaatgc cgggtcgaaa cctcgaacgt gtttgagtac tcgccagttt
    14401 gtcatcgggt cgtaatcaaa ttctttggta atccgttcaa gaattttatg agccgattgt
    14461 gcggtgtggc tatattcaaa accctgtccg tagatttcag gtgcgatatt tgaaccgagc
    14521 ttttgaaaca ttgggtagat atagcggaaa gtgtgaatac ctcgcacgaa agcgaaccaa
    14581 gcccatacga ggctttggag ttcatcttcg gtaaattcaa aggtgtattt cttttcaggc
    14641 agtgcaagcg gtaggttttg ctgattattt tgcaaaagct ctttcatctt gcgttcgcat
    14701 tcgatgaagt attttcggat ttgtctgcct ttttcgttgc gttccaccat tgcgagttct
    14761 ttgcccatat caagggtgat atggtattcc ttgcgtggtc tgccagcggt gcgttcggtg
    14821 acgatgatgt agtcttcgtc ttgcacgaaa ccgtagtcgg tgatgcggtc tgaaatccag
    14881 tgtgaatact gctgtttgct ttcaacgaat gaatgaagct cacgagcatt gcaaagttga
    14941 atttgagtgt tttggattgt accgtcaaaa acggtaataa gtgaatttgt cattttgaca
    15001 ttctccgcta ataagtttta acactcatca ccgcagaggc caatcgttgg tgatgaacta
    15061 gacaggattg gccttacagt tagcggtaac tgcgttcctt tcggaactcc tatctagctc
    15121 atcattggga cttttagata aatttatcta aaaggttgat ttgctaattt cggatataaa
    15181 aaaagacgca tggtgcgtct atcatctctc cgcaccgcta acaattcagg aggccaatcc
    15241 cgactttctg ttgaaagtgc ggttatctta atccgaattg ggggcggttg tcaaacctca
    15301 atatctattt ttgcgatctg catcgaaaaa ataaattttc gttctgacag taaaacgctt
    15361 tcgtagtaat ctcataaaaa acataagggg aacactatga aagagctttt caaacaatgg
    15421 ctaatcaatc aggactcgcc attcattaat agctgtggcg ttgagtgtat tttgtcaaaa
    15481 gttgatgact ggctaaacat aatcaacgcc aacgaagaag aaaccgaaac tcttatagag
    15541 tggcggaatg cttttctgca agatgtttcg gtttttatag cttaggtact ataaatcgtc
    15601 ttctttaacc caaat
//
LOCUS       DANLXF010000044        15599 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.044, whole genome shotgun sequence.
ACCESSION   DANLXF010000044 DANLXF010000000
VERSION     DANLXF010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 15599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15599
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.044"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..738)
                     /gene="dusB"
                     /locus_tag="PXD66_000823"
     CDS             complement(<1..738)
                     /gene="dusB"
                     /locus_tag="PXD66_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDL6319146.1"
                     /translation="MQIGQYAIKNRIFLAPMAGITDQPFRRLCSQLGAGLTFSEMMST
                     NPDVWHTEKSRLRLAHHDEIGLNAVQIAGSDPVEMAKAAKINVEYGADIIDINMGCPA
                     KKVNKNMAGSALLRNPKLVAQILDSVVNAVDVPVTLKIRTGWDQENRNCLQIAKIAEQ
                     AGIKALTIHGRTRSCLFNGEAEYESIKAVKEAISIPVIANGDIDSAEKAKFVLDYTKA
                     DAIMIGRASFGNPWIFKEINDFFETGLS"
     gene            complement(962..1450)
                     /gene="sixA"
                     /locus_tag="PXD66_000824"
     CDS             complement(962..1450)
                     /gene="sixA"
                     /locus_tag="PXD66_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HDL6319147.1"
                     /translation="MNIWIMRHGEAGFNASSDAARCLTDNGIKNTISQGKWLGEHLAN
                     QQIQLDKILVSPYLRAQQTLENVLIGMQAVNFPQHFTNLIESWDEVTPDGNPHTVESY
                     LHFLREEGAKNILLISHLPLVFDLVQILTNNQSNVQFPTSTIAEINWVKTKAELIRVK
                     HT"
     gene            1577..3016
                     /gene="pyk"
                     /locus_tag="PXD66_000825"
     CDS             1577..3016
                     /gene="pyk"
                     /locus_tag="PXD66_000825"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDL6319148.1"
                     /translation="MSRKLRRTKIVCTMGPATDRGNTLEKIIAAGANMVRMNFSHGIP
                     EDHIERANKVREIAARLGRHVAILGDLQGPKIRVSTFKDGKIFLNIGDKFTLDADLPR
                     GEGHQEAVGLDYKNLPNDVVPGDILLLDDGNVQLKVLEVEGVKVHTEVTVGGPLSNNK
                     GINKLGGGLSAPALTDKDKEDIKLAAKIGVDYLAVSFPQSSADLHYARELAKEAGLEA
                     KIVAKVERAETVATEEAMDDIILGADVVMVARGDLGVEIGDAALVGVQKRLIRRARKL
                     NRVVITATQMMESMIKKPMPTRAEVMDVANAVLDGTDAVMLSGETANGDYPVETVKSM
                     AEVCLGAETMPSINISRHRMEGTFKSIEEAVAMSAMYTANHLEGVSAIISLTHSGETA
                     KLTSRISSGLPIYAMSRNQKALNLCALYRGVTPVYYNEDSRTMEGAKKAIAQLKEQGY
                     LLTGELVLLTHGDELKAGGTNTCRILRVE"
     gene            3112..4149
                     /locus_tag="PXD66_000826"
     CDS             3112..4149
                     /locus_tag="PXD66_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDL6319149.1"
                     /translation="MHNKLLSICILRLSAIGDVCHTLAVIQAIQRKYPSAEITWIIGK
                     TEAYLMKDIPNVHLIPYDKKIGWKGIFTLWQKLSHKRFDFLLNLQTAFRASILSLGIK
                     AKKKIGFNQDRAREMQWLFTNQKVEMTDSPHVLDGQMMFAKAIGIMDLSAQWNLPVSD
                     EDLAYGKQFIDFTRKNILIAPCSSKKEKDWLPERYAEIANWLSKQNINVLIAGSPSQY
                     EMETAAKIQQLAPNCTSIAGKTTLKQLAGVIRQVDLVISSDSGPAHIATTQNTPVIGL
                     YAIHNPRRTGPYNDLDKVVSVYDEAILQSYGKPWQQLAWATKAKGKNWMEKITVESVK
                     QKVVETLKITL"
     gene            4189..5247
                     /locus_tag="PXD66_000827"
     CDS             4189..5247
                     /locus_tag="PXD66_000827"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDL6319150.1"
                     /translation="MNTVFNQDSVHNVNIQDEKVLLTPQELKQEFPLPEHLRNQIERS
                     RKTISDIIHRRDKRQLIVIGPCSIHDPVAALEYGKKLKALSEQVSDKLYIVMRVYFEK
                     PRTTVGWKGLINDPKMDGSFDVETGLRIARKLCLDLAELGLPLATEALDPMTPQYLAD
                     LFSWSAIGARTTESQTHRELASGLSMAVGFKNGTDGDLAVALNAMQSSAQSHSFIGIN
                     QQGQVTLLKTKGNADGHIILRGGKTPNYEKQYVDDCEWVLKQANLPTAIMIDCSHGNS
                     NKDYRRQPLVAENVLEQLLAGNTSIIGLMIESNLHAGNQSSELPFEQMKYGVSITDAC
                     IDWQTTEELLTHFAEKLR"
     gene            5302..6423
                     /gene="tyrA"
                     /locus_tag="PXD66_000828"
     CDS             5302..6423
                     /gene="tyrA"
                     /locus_tag="PXD66_000828"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HDL6319151.1"
                     /translation="MNPLAPLREQIDQVDQQLIALLSERLALVAEVGKVKSEHGIPVY
                     APERETAMISARRSEAEKQGIPADLIEDVLRRIMRESYANENKHGFKRVNSDIQKIVI
                     VGGKGKLGGLFGNYLTLSGYNVEALGRDDWDNAEQILANADVVIVCVPIANTLATIER
                     LQSYLTENMILADLTSVKHQPLQKMLEVHNGAVVGLHPMFGPDIASQAKQVVACCHGR
                     FPERYQWLLEQIKIWGAKIEEIEAAAHDNAMTYIQALRHFSTFAFGLHLSRQPVQLSQ
                     LLALSSPIYRLELAMIGRLFAQDGSLYADIIADKPENLATIETLKESFEQGLDFFKRN
                     DKAGFIKAFEEVHHWFGDYSEQFLKESRVLLQQAHDSRK"
     gene            complement(6614..7699)
                     /gene="recF"
                     /locus_tag="PXD66_000829"
     CDS             complement(6614..7699)
                     /gene="recF"
                     /locus_tag="PXD66_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDL6319152.1"
                     /translation="MALTRLLINHFRNIQHTDLAFSPHFNFLVGANGSGKTSLLESIF
                     YLGHGRSFKSHISNRIIHYDKDDFVLHGKIDEAKHSWSVGIQKFRSGETTLKINGEDG
                     NKIADLAHLLPMQVITPEGLTLLNGGPSFRRAFLDWGLFHQHPDFYAHWNNLRRLLKQ
                     RNSALQQVRSYQELKAWDIELVKTTYAVSEMRAEYAEALRPEIEKTCQFFLPEIEIGL
                     SFHQGWEKGADYAEILAQGFERDKALGYTMIGAQKADFRFKANGLPVEDVLSRGQLKL
                     LMCALRLAQGEHLVAQKQRQCLFLIDDFASELDPTKRELLAHRLRESGSQVFVTAITQ
                     DQLNQMQWQDRSDDCLFTVKNGEIQPQ"
     gene            7839..8168
                     /locus_tag="PXD66_000830"
     CDS             7839..8168
                     /locus_tag="PXD66_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDL6319153.1"
                     /translation="MFGKGGLGGLMKQAQQMQERMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRIEIDPSLMEDDKEMVEDLVAAAFNDAVRRAEEMQKEKMASVTAGMQLP
                     PGMKFPF"
     gene            8176..8781
                     /gene="recR"
                     /locus_tag="PXD66_000831"
     CDS             8176..8781
                     /gene="recR"
                     /locus_tag="PXD66_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015939588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDL6319154.1"
                     /translation="MQTSPLLENLMEALRALPGVGPKSAQRMAYHLLQRNRSGGVALS
                     KALNEAMSHIGHCKSCRTFTEEEECTICKNPRRQMSGQLCVVEMPEDIQAIEQTGQFS
                     GRYFVLMGHLSPIDGIGPREIGLDLLQQRLEQESFHEVILATNPTIEGDATANYIAEM
                     CRMYNVKATRIAHGVPVGGSLEMVDGTTLSHSFAGRRDIDI"
     gene            8853..9179
                     /gene="grxD"
                     /locus_tag="PXD66_000832"
     CDS             8853..9179
                     /gene="grxD"
                     /locus_tag="PXD66_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005705415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HDL6319155.1"
                     /translation="METIDKIKQQISENPILLYMKGSPKFPSCGFSARAVEAVINCQV
                     PFGYVDILTNPDIRAELPKFANWPTFPQLWVEGELVGGCDIVLEMLQKGELQTLLKET
                     AAKHAA"
     gene            9321..10358
                     /locus_tag="PXD66_000833"
     CDS             9321..10358
                     /locus_tag="PXD66_000833"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HDL6319156.1"
                     /translation="MALKELDWANLGFSYIKTDYRFIAHWKDGKWNEGELTQDNTLHI
                     HEGSTALHYGQQCFEGLKAYRCKDGSINLFRPDQNAERMQRTADRLLMPRVPTELFIR
                     ACKKVVKANQDWLGPYGSGATLYLRPFLIGVGENIGVKAAPEFIFSVFCCPVGAYFKG
                     GLAPSNFITTDYDRAAPMGTGGVKVGGNYAASLLPHELAVEQSSTTRKFADAIYLDPK
                     THTKIEEVGAANFFGITKDNKFITPISESILPSITKYSLLHLAQERLGMEAIEGDVYI
                     DQLDQFAEAGACGTAAVITPVGGIQHKDKFHVFYSETEVGPVTRRLYAELTGIQFGDV
                     EAPQGWIVKVE"
     gene            10385..10933
                     /locus_tag="PXD66_000834"
     CDS             10385..10933
                     /locus_tag="PXD66_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane lipoprotein lipid attachment
                     site-containing protein"
                     /protein_id="HDL6319157.1"
                     /translation="MAKLFANSTACLYLENMKKLIFLGLSALALAGCSSTISNPNQLI
                     GQWQCTIEYDDFNIRTVDNLRFSTNGTMTNQGGIYYPIQKSIFEYSLQQNGRWSVKNG
                     AIVYRIVSESVQRSHRSQIWSELQRDAELQQFEENLFSSLSDSDNNKTVELIITDFDE
                     KEMDIKQEIKGHKAYKGQCIKQ"
     gene            10942..11286
                     /locus_tag="PXD66_000835"
     CDS             10942..11286
                     /locus_tag="PXD66_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDL6319158.1"
                     /translation="MIQRFEVGSRFSEMAIHNGVAYLAGQVPLDDTADAYTQTQQVLA
                     EIDKWLAKANSDKSKILMATVYLKEMADYAEMNRAWDEWVAPNNAPPRAAVEAKLANP
                     NWKVEIVVTAAV"
     gene            complement(11319..13097)
                     /locus_tag="PXD66_000836"
     CDS             complement(11319..13097)
                     /locus_tag="PXD66_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HDL6319159.1"
                     /translation="MSTLDFHFIHRFHQQVQALSHHPALRFMQDEKWSEISWLELQKQ
                     VNNLSYALLANGLAVQEKIAIFAQNMPRWTITDIAAMQIRAVAVPIYATSTTKQLEYI
                     LNDGDIKILFVGDQEQYDQAIEIANNCSLLIKIVAMKDSIDLRDFPIAQHWQDFIKVN
                     QDEAELTKRLNDKCLEDLFTLIYTSGTTGEPKGVMLDYANLAHQLQAHNKALPHIDEN
                     DISLSFLPLSHIFERAWVTYVLYRGAVNCYLEDPQQVRQALSTVRPTLMCAVPRFYEK
                     IYTAVWDKVQQAPIHRRALFKWAIRTAEDNLFGKKSAFFHRLQYIMADKLVLGKLRAL
                     LGGRIRMMPCGGAKLDPSIGLFFQSIGINVKLGYGMTETTATISCWQEKGFNINSTGT
                     LMPDVEVKISEENEILVRGGLVMKGYYKKPEATAQAFTAEGFLKTGDAGELDSEGNLY
                     ITDRIKELMKTSNGKYIAPQYIEGKIGKDKFIEQIAVIADAKKYVSALIVPCFDSLEE
                     YAKKLNIRYQDRLELIKHYEVVRMFEQRINELQKDLASFEQIKKFTLLSQAFSTKMEE
                     ITPTLKLRRKVILERYKQQIEKMYES"
     gene            complement(13323..13892)
                     /locus_tag="PXD66_000837"
     CDS             complement(13323..13892)
                     /locus_tag="PXD66_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005821628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HDL6319160.1"
                     /translation="MNQENHKIEDIIRIFNQCFSDEYNTRLERGGNYPIYLPEFMDED
                     GVPSERPYNVILFAHGYYSSALHEIAHWLVAGEARRKLEDFGYWYEPDGRSAERQREF
                     ESAEIKPQAIEWVLATAAGFRYFASADNLSGNPGDNSAFKQAVYDQVKLYAERGYLPK
                     RAETLRKALCAFYGTPDVIDLAQFDIERI"
     gene            complement(13902..>15599)
                     /locus_tag="PXD66_000838"
     CDS             complement(13902..>15599)
                     /locus_tag="PXD66_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HDL6319161.1"
                     /translation="FSQPKFLKSEEAFVFSIQNENETLQLNWDIADNYYLYKKEIRVT
                     PQNIEIGDITFPAAEQYNDEFFGQVEIYRNHLGLKVPFKEIKDNASIVVEYQGCTKGF
                     CYPPERVELKLDSILSATPEELAQLEAQAVEKSENFAKTDNMAPPENVAKAEQDQLAE
                     NLANNRFSIFWFFVLGVGLAFTPCVLPMLPLLSAIVIGNKQRPSTAKALLLSIAYVQG
                     MAVTYTLLGLIVAAIGLPFQIALQSPPVLIGLSIVFILLACSMFGLYEIQLPNSWQQK
                     LNAMSQKQQGGALGSVFLMGMIAGLIASPCTSAPLSGALLYVAQSGDLVTGGLALYLL
                     ALGMGLPLILITLFGNKILPKSGEWLLKVKAAFGFVMLALPVFLLSRVLPSHYEPFLW
                     SALAVAFLIWLVDAIPSNSITKKIIKVLLLLALAIAAKPWADLAWNGSTTQQQTTPHL
                     ALEKIQSLADLETKLTASKGKKVMLDLYADWCVACKEFEKYTFSDQNVQNKLNEMVVL
                     QIDMTKNSAENIELMKHFNVLGLPTILFFDENGNEMSQSRVTGFLDAEQFLAWLNKL"
BASE COUNT         4680 a         3016 c         3344 g         4559 t
ORIGIN      
        1 agataatcca gtttcaaaaa aatcattaat ttccttaaat atccaaggat ttccaaagct
       61 agcgcgccca atcatgattg catcggcttt ggtatagtcg agaacaaact ttgctttttc
      121 cgcagagtca atgtctccgt ttgctatcac tgggatagaa attgcttctt ttactgcctt
      181 aatactttca tattcagctt cgccattaaa taaacagctt cgagtacgcc cgtgaatagt
      241 gagagctttt atgccggctt gttctgcaat tttcgcaatt tgcagacaat ttctattttc
      301 ctgatcccaa cctgttctaa tttttagtgt cacagggaca tcaacggcat tgactacaga
      361 atctaatatt tgagcgacta atttggggtt acgtaaaaga gcagagcctg ccatattttt
      421 attcaccttt tttgccgggc aacccatatt aatatcaata atatcggcac catactcaac
      481 atttatcttt gcagcctttg ccatttcaac aggatcagaa cctgcaattt gtacggcatt
      541 taagcctatt tcatcgtggt gtgctaaccg caatcgtgat ttctcagtat gccatacatc
      601 aggattggta gacatcatct ctgaaaatgt taaacctgct ccaagttggc tacataagcg
      661 gcgaaaaggc tgatctgtga ttccagccat aggtgcaagg aaaatacgat ttttgattgc
      721 atattgtcct atttgcattt ttaacctttt attaacaaat ttgaagtaat tttaaccaga
      781 gggatatcca aataaaaagt acggtaaata ccgtactttc ttataatagc ttatggatca
      841 gtttagtact gatttgaggg ctggtatgat acgcattttt taggtatttt ggaagtctat
      901 aaattcgaca atttataaaa aaattgaaaa tttttatatt gacattctaa aaatgaaaga
      961 attaagtatg ctttactcga attagctcag cttttgtctt tacccaatta atttctgcta
     1021 tggttgaagt tggaaattgt acattgcttt ggttattcgt taaaatttgg acgagatcga
     1081 acacaagtgg gagatgtgaa atcagaagaa tattttttgc gccttcctct cttaaaaaat
     1141 gtagatagct ttctactgta tgcgggtttc cgtcaggcgt aacctcgtcc caactttcaa
     1201 tgaggttggt aaaatgttgg ggaaaattga ccgcttgcat accaataagc acattttcaa
     1261 gcgtttgttg cgccctaagg tatgggctga cgagaatttt atctaattga atttgttggt
     1321 tggctaaatg ttctcctaac cattttccct gtgaaatggt gttttttata ccattgtcgg
     1381 ttaaacagcg agcagcatca ctcgaagcat taaagcctgc ttccccatgg cgcataatcc
     1441 aaatattcat aacattctcc tttagttgta tttaaactct cttgatttag gcgtataatg
     1501 accctcgttt aacgcataat caagatgctt agaaagattt taacttaatt tttaaattta
     1561 ttgatggagt caaataatgt ctagaaaact cagaagaacg aagatcgttt gtacaatggg
     1621 acctgcaaca gatcgtggca atacgttgga aaaaatcatt gctgccggtg caaatatggt
     1681 tcgtatgaat ttctctcacg gtattcctga agatcacatc gaacgtgcga ataaagtgcg
     1741 tgaaattgct gcaagattag gtcgccacgt tgcgatttta ggcgatttgc aaggtcctaa
     1801 aattcgtgtt tcgacattta aagacggtaa aattttctta aatattggtg ataaattcac
     1861 cttagacgca gatttaccgc gtggagaagg ccatcaagaa gctgtaggtt tagattacaa
     1921 aaacttacca aatgatgtag tacctggtga tattctattg ttagatgacg gtaatgttca
     1981 actgaaagtg ttggaagtgg aaggcgttaa agttcatact gaagtgacag ttggtggccc
     2041 gttatcaaac aataaaggga ttaacaaatt aggtggtggt ttatcagctc ctgcattaac
     2101 cgacaaagat aaagaagaca ttaaattagc ggcgaaaatc ggtgtggatt acctagcggt
     2161 ttcattccct caatcaagtg cagatttaca ttatgcacgt gaattagcaa aagaagcagg
     2221 cttagaagcg aaaattgtag cgaaggttga acgtgccgaa actgttgcga ctgaagaagc
     2281 aatggacgac atcattttag gtgcggacgt tgtgatggtt gctcgtggtg acttaggggt
     2341 tgaaattggt gatgcggctt tagtgggtgt gcaaaaacgt ttaatccgcc gtgctcgtaa
     2401 gctaaatcgt gtggtaatta ccgcaaccca aatgatggaa tcgatgatta aaaaaccaat
     2461 gccgacccgt gctgaagtga tggatgttgc aaatgctgtg ttagatggta ctgatgcggt
     2521 aatgctttcc ggcgaaactg caaacggtga ctaccctgtt gaaacggtaa aatcaatggc
     2581 agaagtgtgc ttaggtgctg aaacaatgcc aagcattaac atttctcgcc accgtatgga
     2641 aggtacgttt aaatctatcg aagaagctgt ggcaatgtct gcaatgtaca ccgcaaacca
     2701 tttagaaggt gtatcggcaa ttatttcatt aactcattca ggcgaaacgg ctaaattaac
     2761 cagccgtatc agctcaggtt taccgattta tgcgatgtct cgtaaccaaa aagcattaaa
     2821 cctttgtgcg ttatatcgtg gtgtaacacc ggtatattac aatgaagaca gccgcacaat
     2881 ggaaggggcg aaaaaagcca ttgctcaatt aaaagaacaa ggctatttat taaccggcga
     2941 attagtgctt ttaactcacg gagatgaatt aaaagctggc ggtacaaata cttgtcgtat
     3001 cttaagagtt gaataatcta aacgattaac gcaaaagcca cataaaacac tttatgtggc
     3061 tttttttctg tcttaatata gcttaatgta atctgaacta ttgatattat tatgcacaat
     3121 aaacttcttt ctatctgtat tctccgcctt tctgccattg gcgacgtttg ccatacctta
     3181 gcggttattc aagctattca acgaaaatat cctagtgctg aaattacttg gattatcggc
     3241 aaaacggaag cgtatttaat gaaagatatt ccgaatgttc acttaattcc ttatgataaa
     3301 aaaatagggt ggaaaggtat ctttacactt tggcaaaaac tttctcacaa gcggtttgat
     3361 tttttattaa atttacaaac ggcattccga gcttcaattc tatcgcttgg gattaaagca
     3421 aagaaaaaaa tcggctttaa tcaagacaga gcaagggaaa tgcaatggtt gtttaccaac
     3481 cagaaagtag aaatgacaga ctctcctcac gtgcttgacg ggcaaatgat gtttgccaaa
     3541 gccattggga taatggattt atcggcacaa tggaatttac ctgtgagtga tgaagattta
     3601 gcttatggca aacaatttat tgattttaca aggaaaaata tactcattgc tccttgttca
     3661 agcaagaaag agaaagattg gttgccggaa cgctatgcag aaattgctaa ctggctaagt
     3721 aagcaaaata tcaatgtatt aattgcaggt tctccatcgc aatatgaaat ggaaaccgca
     3781 gccaaaattc aacaacttgc cccgaattgt acgagtattg ccggtaaaac cacgctaaaa
     3841 cagcttgcag gtgttattcg tcaggttgat ttggtgatct cttcagattc aggtcctgcc
     3901 catatcgcca ctacgcaaaa tacgccggtg attgggttgt atgcgattca taatccaaga
     3961 cggacaggcc cttacaatga cttagacaaa gtggtttcgg tatatgatga agcgattttg
     4021 caatcttatg gtaaaccttg gcagcaatta gcttgggcaa ctaaagctaa aggcaaaaat
     4081 tggatggaaa aaatcacggt ggagagtgtg aaacaaaaag tagttgaaac tttaaaaatt
     4141 acgttgtaaa gtttgtgtat taaaagattt acacaaaagg aaattaaaat gaacacagtg
     4201 tttaatcaag atagcgtgca taacgtaaat atccaagatg aaaaggtatt actgacccca
     4261 caagagttaa aacaagaatt tccgttgccg gaacatttac gcaatcaaat tgaacgttca
     4321 cgcaaaacga tttccgatat tattcacaga cgagataaac gccaactgat tgtgattgga
     4381 ccttgttcga ttcacgaccc agttgctgcc cttgaatatg gtaaaaaatt aaaagctctt
     4441 tcggagcaag tgagtgataa gttgtatatt gtgatgcggg tttattttga aaagccccgc
     4501 actactgtag gttggaaagg gctgattaac gatccgaaaa tggacggctc ttttgatgtc
     4561 gaaaccggct tacgtatcgc acgaaaactc tgtttggatt tagccgaact tggcttgccg
     4621 ttagcgaccg aagccttaga tccgatgaca ccacaatatt tagcggattt gttcagctgg
     4681 tcggcaattg gagcaagaac aactgagtct caaactcacc gtgaattagc ctcaggtttg
     4741 tcaatggcgg taggctttaa aaatggtaca gatggtgatt tagccgttgc acttaatgcg
     4801 atgcaatctt ccgctcaaag ccatagcttt atcggcatta accaacaggg gcaagttact
     4861 ttactcaaaa caaaaggtaa tgcagacgga catattattt tacgtggcgg taaaacccca
     4921 aattatgaaa agcaatatgt tgatgattgt gaatgggtgc taaaacaagc aaacttacct
     4981 acagcgatta tgattgattg cagccacggc aattccaata aagattaccg ccgccaaccg
     5041 ctggtggcag aaaatgtatt agagcagcta cttgctggca atacttcgat tatcggttta
     5101 atgattgaaa gcaatttaca cgcaggtaat caatcatcag aattgccgtt cgagcagatg
     5161 aaatacggtg tttctattac ggatgcttgt attgactggc aaactacaga agagctttta
     5221 actcactttg ctgaaaagtt gcgttaaaga taatttgcaa aaaattgcat aaaaacgacc
     5281 gcttgtaaat tggtaacaaa tatgaaccca ttagccccat tacgagaaca aattgatcaa
     5341 gttgatcaac aacttatcgc cttactttct gaacgcttag ctcttgtggc agaagttggg
     5401 aaagtcaaat cagaacacgg cattcctgtt tatgctccgg agcgagaaac cgcaatgatt
     5461 tccgcccgcc gctctgaagc ggaaaaacaa ggtattccgg cggatctcat tgaagatgtg
     5521 ctacgccgta taatgcgtga atcttatgcc aatgaaaaca aacacggctt caaaagagtt
     5581 aattcggaca ttcaaaaaat cgtgattgtt ggtggtaaag ggaaattagg tgggctgttt
     5641 ggcaattatt taaccctatc cggctataat gttgaggcat taggacgtga tgattgggac
     5701 aatgccgagc agattttagc caatgcagat gtggttattg tgtgtgtgcc gattgccaac
     5761 accctagcaa ccatcgagcg tttacaatct tatttaaccg aaaatatgat tttggcggat
     5821 ttaacctccg tcaaacatca gccgttgcaa aaaatgctgg aagtgcataa tggagcggta
     5881 gtcggtttac acccgatgtt cggaccggat attgcaagtc aggcaaagca agtggtcgct
     5941 tgctgccacg ggcgtttccc tgaacgttat caatggttgc ttgagcaaat caaaatttgg
     6001 ggggcaaaaa ttgaagagat tgaggcagca gcacacgata atgcaatgac ctacattcaa
     6061 gccctacgcc acttttccac ttttgcattc gggctacatc tttctcgcca gccggtgcaa
     6121 ctctctcaat tattggcact atcttcgccg atttaccgct tagagttggc aatgattggg
     6181 cgtttatttg cccaagacgg cagcttatat gccgatatta ttgccgataa accggaaaat
     6241 ttagccacca tcgaaacctt aaaagagagt tttgaacaag ggctagattt ctttaaacgc
     6301 aacgacaaag ccggttttat caaggctttt gaggaagtgc atcactggtt tggcgattat
     6361 tccgagcagt tcttgaaaga gagtagagta ttgctccaac aggctcacga ttcaagaaag
     6421 taaaggtagg ttgaggtttt gctctgatag cactagcgtc cacgctagtg ctatttaata
     6481 ttctacaagc ggttaataaa gagctgtttt ttgcaaattt ttatgcaaaa ttatccgctt
     6541 gttttggcac gagcgtggac gctcgcgcca tcggaagaaa catttttgta aaattgcccc
     6601 caaatctgac cgcttactgt ggctgaattt ctccattttt taccgtaaac aaacaatcat
     6661 cgcttctgtc ttgccattgc atttgattga gctgatcttg agtaatggcg gtaacgaata
     6721 cttgtgagcc actttctctt aaacgatgtg ctaaaagctc acgtttggtt gggtcgagtt
     6781 cggaggcaaa atcgtcaatt aaaaataggc attggcgttg tttttgggcg accaaatgtt
     6841 cgccttgagc cagtcttaac gcacacatca atagctttaa ttgcccacgg gaaagcacat
     6901 cttccaccgg taagccgttc gctttaaaac ggaaatcggc tttttgagct ccaatcatcg
     6961 tatagccaag agctttatcc cgctcaaaac cttgtgccag aatctcggca taatccgccc
     7021 ctttttccca gccttgatga aagctcaagc cgatttcgat ttccggcagg aaaaattggc
     7081 aggttttctc aatttcgggg cgtaacgctt cggcgtattc ggctcgcatt tcgctcacgg
     7141 cataagtggt tttcaccaat tcgatatccc acgctttgag ttcttgataa gagcggactt
     7201 gttgcaaagc cgaattacgt tgctttaata aacggcgaag attgttccaa tgagcgtaga
     7261 aatcagggtg ctggtgaaat aagccccagt ccaaaaaagc acggcgaaag ctcggaccac
     7321 cgttaagtaa ggttaagcct tcgggggtga tcacttgcat cggcaataaa tgggctaaat
     7381 cggcaatttt gttgccgtct tcaccgttga ttttaagtgt cgtctcgcca ctgcgaaatt
     7441 tctgaatgcc gaccgaccaa ctgtgctttg cctcgtcaat tttaccgtgt agtacgaaat
     7501 cgtctttatc gtaatggata atgcggttgc tgatatggct tttgaatgaa cgcccgtgtc
     7561 caagatagaa aattgattcc aacaaactgg ttttgccact tccgttcgcc ccgactaaaa
     7621 aattaaaatg cgggctaaat gctaaatcgg tatgttggat atttctaaaa tggttgatta
     7681 ataaacgggt taaagccata gttaaagtgc gaggaataaa aagagtagcc attttagcaa
     7741 atttgcaaaa ttttgggaaa aattaaccgc ttgcggatta gaaagtggtt cagtagaata
     7801 agccgtttat tttttattga aaattaagag gaaaaattat gtttggaaaa ggtggcttag
     7861 gcggcttaat gaaacaggct cagcaaatgc aagagcgtat gcaaaaaatg caagaagaga
     7921 tcgcccaatt agaagtaaca ggcgagtctg gtgcgggttt ggtaaaagtc actattaacg
     7981 gggctcacaa ttgccgccga attgaaattg atccatcatt aatggaagat gataaagaga
     8041 tggtggaaga tttagttgcc gctgccttta acgatgcggt tcgccgtgca gaagaaatgc
     8101 aaaaagagaa aatggcaagt gtgactgccg gtatgcaact gccgccgggt atgaaattcc
     8161 cattctaatt taagtatgca aaccagtcct ttacttgaaa atttgatgga agccttgcgt
     8221 gcattgcccg gtgtcggacc gaaatcggca cagcgaatgg cttaccatct gttgcaacgt
     8281 aatcgttccg gcggtgtggc gttgtcaaaa gccttaaatg aggcaatgag ccatatcgga
     8341 cattgcaaat cgtgccgtac ttttaccgaa gaggaagagt gtacgatttg taaaaatcct
     8401 cgccgtcaga tgagcgggca actttgtgtg gtagaaatgc cggaagacat tcaagcgatt
     8461 gagcaaaccg ggcaattttc agggcgttat tttgtgctga tggggcattt gtcgccgatt
     8521 gatggtatag gccctcgtga aatcggctta gatttattgc aacaacgctt ggaacaggaa
     8581 tctttccacg aagtgatttt ggcgactaat ccgaccattg aaggtgatgc taccgcaaac
     8641 tacattgctg aaatgtgcag aatgtataac gtgaaagcta ctcgtattgc tcacggtgtg
     8701 ccggtcggtg gttcattaga aatggttgat ggcacaacgc tttctcactc ttttgcagga
     8761 cggcgtgata ttgatattta aagctatttc tgtgctaact taattctgat ataattactc
     8821 aactatttta aattattatt ttgaggctaa aaatggaaac tattgacaaa atcaaacaac
     8881 aaatcagcga aaatccgatt ctactttata tgaaaggttc accgaaattc ccatcttgtg
     8941 gtttctccgc tcgtgcggtg gaagcggtaa ttaactgcca agtgcctttc ggttatgtgg
     9001 atattttaac caatccggat attcgtgcgg aattgccaaa atttgcaaac tggccgactt
     9061 tcccacaatt atgggtagaa ggtgaattag ttggcggttg tgacatcgta ttagaaatgc
     9121 tccaaaaagg cgaacttcaa accttattaa aagaaaccgc agcaaaacac gcagcataat
     9181 ttcaaaccag tatgttaagg ctcttatgtt taagagcctt aattttttct tgcgatttag
     9241 gcttaaactt cttacctcaa gagctatttt gttttagaat gtcaaaatta tttcgtaaca
     9301 taaaaataaa aggacacaac atggcattaa aagagttaga ttgggcaaat ttaggctttt
     9361 cttatatcaa aaccgattac cgtttcattg cacattggaa agacggaaaa tggaatgaag
     9421 gcgaattaac tcaagacaat acactgcata ttcacgaagg ctcaactgcc ctgcactatg
     9481 ggcaacaatg ttttgagggg ttaaaagcct atcgctgtaa agatggctca attaacttat
     9541 tccgcccgga tcaaaatgcc gagcgtatgc aacgcaccgc agatcgttta ttaatgccac
     9601 gtgtgccaac cgagctgttt attcgtgcct gtaaaaaagt ggtaaaagcc aatcaagatt
     9661 ggttagggcc ttacggttcg ggggcaaccc tttatttacg tccgttttta atcggcgtgg
     9721 gcgaaaatat tggggtgaaa gctgcacctg aatttatttt ttccgtattc tgctgtccgg
     9781 tgggggcata tttcaaaggt ggcttagcac cctcaaactt cattacaacc gactatgacc
     9841 gtgcagcccc aatgggaaca ggtggtgtga aagtaggcgg taactacgct gcgagtttat
     9901 taccgcacga attggcggtt gagcaaagtt caactacccg taaatttgcc gatgcgattt
     9961 atttagatcc gaaaacccat accaaaattg aagaagtggg ggcggcgaac ttctttggga
    10021 ttaccaaaga caacaaattc atcaccccga tttcagagtc gattttgcca agtattacca
    10081 aatactcatt gctacacctt gctcaagagc gtttaggtat ggaagcgatt gagggagatg
    10141 tgtatatcga ccagttagac caatttgccg aagccggtgc ttgtggtacg gctgcggtga
    10201 ttacgccggt tggtggtatt caacataaag acaaattcca cgtcttctat tcagaaaccg
    10261 aagtggggcc tgtcacccgc cgtttatacg cagaattaac cggtattcaa tttggcgatg
    10321 ttgaagcacc gcaaggctgg attgtaaaag tagagtaatt ttacacaagt aacaagcggt
    10381 cgaattggca aaactttttg caaattcaac cgcttgtctt tatttagaga atatgaaaaa
    10441 gttaattttt ttaggtttat cagcacttgc attagctggt tgttcctcaa caatttctaa
    10501 tcctaatcaa ctgattgggc aatggcaatg cactattgag tacgatgatt ttaatattcg
    10561 tacggtggat aatttacgat tttccacaaa tgggactatg accaatcaag gtggtatata
    10621 ttaccctatt caaaagtcga tttttgagta ttcattacaa cagaatgggc gctggagtgt
    10681 gaaaaacggg gctattgttt atcgtatcgt ctcagaatct gtacagcgta gccatcgttc
    10741 acaaatatgg tctgaattac aacgtgatgc ggagctacaa caatttgaag agaatttatt
    10801 tagctcactt tctgatagtg ataataataa aacagtggaa ctaatcatca ctgattttga
    10861 tgaaaaagaa atggatatca agcaagaaat aaaagggcat aaagcttata aagggcaatg
    10921 tataaaacag tagaggtaaa aatgattcaa cgttttgaag taggtagccg tttttcggaa
    10981 atggcaatac ataacggtgt ggcttacttg gcagggcaag tgccgttaga tgacaccgcc
    11041 gatgcttaca cccaaaccca gcaagtgctt gcagaaattg ataaatggct ggcaaaagcc
    11101 aacagcgata aaagtaaaat tctaatggct acggtttatt taaaagagat ggcagattat
    11161 gcggaaatga accgtgcgtg ggacgaatgg gttgccccaa ataacgctcc accaagagcg
    11221 gcggtggagg ctaaattagc caatccaaat tggaaagtcg aaattgtggt taccgctgcg
    11281 gtataaatga aaagcagaaa gacgatctct gtttttcatt atgattcgta cattttttcg
    11341 atttgttgct tatagcgttc tagaatgacc tttcgtctta attttaatgt aggcgtaatt
    11401 tcttccattt tggtgctaaa tgcttgcgaa agtagtgtaa actttttgat ctgttcaaaa
    11461 cttgcaaggt ctttttgtaa ttcgttaatg cgttgttcaa acattctgac aacctcataa
    11521 tgtttaatga gttctaatct gtcttgatag cgaatattga gcttttttgc atactcttcg
    11581 aggctatcaa agcaaggaac tatcaacgca gaaacatatt ttttggcatc agcaatcacc
    11641 gcaatttgct caataaactt atctttacct atttttcctt caatgtattg tggggcaata
    11701 tatttaccgt ttgaagtttt cattaattct ttaatacgat cggtaatata aagattgcct
    11761 tccgaatcca gttcaccggc atctcccgtt tttagaaaac cttcagcagt aaaggcttga
    11821 gcagtggctt ccggtttttt gtagtaacct ttcataacca agcctccacg cactaaaatt
    11881 tcattttctt cactaatttt gacctcaaca tcaggcatta aggtaccggt tgagttgata
    11941 ttaaagcctt tttcctgcca gcatgaaata gtggcagtag tttcggtcat tccgtatcca
    12001 agttttacat tgatgcctat actttggaaa aatagcccga tagacggatc taatttagct
    12061 cctccacacg gcatcatacg aatacgaccg cctagtaagg ctcgtaattt tcctagcacc
    12121 aatttatctg ccataatgta ctgcaagcgg tggaaaaagg ctgatttttt accaaacaga
    12181 ttgtcttcgg cggtacggat tgcccattta aataacgctc ggcgatgtat cggtgcttgt
    12241 tggactttat cccacaccgc agtatagatt ttttcataga accttggtac ggcacacatt
    12301 aatgtaggtc gtaccgttga gagtgcttga cgtacctgtt gaggatcttc cagatagcaa
    12361 tttactgccc ctctgtatag cacataagtg acccaagcac gttcaaagat atgcgataaa
    12421 ggtaaaaatg aaagggatat atcattttcg tcaatatgag ggagtgcttt attatgtgcc
    12481 tgaagttgat gagctaaatt agcataatct aacataacac ctttaggctc accggttgtt
    12541 cccgaggtat aaattaaagt gaataaatcc tctaagcatt tatcatttaa acgtttggtt
    12601 aattcagctt catcttgatt aaccttaatg aaatcttgcc aatgttgagc gatagggaaa
    12661 tcacgcagat ctattgagtc tttcatcgcg acaattttta ttaatagcga acagttgttc
    12721 gctatttcta tggcttgatc atattgttcc tgatcgccaa caaagaggat ttttatatcc
    12781 ccgtcattca ggatatattc aagttgtttg gtggtactgg ttgcataaat cggcactgct
    12841 accgcacgaa tttgcattgc agcaatatcg gtaattgtcc aacgaggcat attttgtgca
    12901 aaaatagcga ttttctcttg aacggcaaga ccgtttgcca gcaaggcata agaaagatta
    12961 ttgacctgtt tttgtaattc taaccaactt atttctgacc atttttcatc ttgcataaaa
    13021 cgtaaagctg ggtggtggct aagagcctga acttgctggt ggaaacggtg gataaagtga
    13081 aaatctaaag tgctcataat cttattttta ctttctattt cagtgaacag ttgtacgcta
    13141 atataataga aaatatgaaa aaaagaaaca gttgtactct gaaacaaagg gttttggata
    13201 ggaagtaatc aaggcaatag aaatagataa gtagggtatc gtttagtaca aatgcaatat
    13261 ttttcaaaaa tgagaccgct tgtatatgca acaagcggtc tgttttgtta aaaattttgc
    13321 aactaaattc gttcaatatc aaattgagct aaatcaatca catcaggtgt accgtaaaaa
    13381 gcacagaggg ctttacgcaa ggtttcagct cgtttgggaa ggtagccacg ttctgcatag
    13441 agcttgactt ggtcgtacac tgcttgttta aaggctgaat tatcgccggg attgcccgag
    13501 agattgtcgg cactagcaaa ataacgaaaa ccggcagcag ttgctaacac ccattcaatc
    13561 gcttgcggtt tgatttccgc actttcaaat tcacgttggc gttcggcaga gcgtccgtcc
    13621 ggctcgtacc aatagccaaa atcttcaagt ttacgccgag cttctccagc aacgagccaa
    13681 tgggcaattt cgtgcaaggc actgctgtaa tagccgtgag caaataaaat cacattgtaa
    13741 gggcgttcgc tcggcacacc atcttcgtcc ataaattcgg gcaagtaaat cggataatta
    13801 cccccccgct ctaggcgagt gttatattca tcagaaaagc attgattaaa aatgcgaata
    13861 atgtcttcaa ttttatgatt ttcttgattc atttcttttc tttataactt attcagccaa
    13921 gctaaaaatt gctcggcatc caaaaagcca gtaacacgag attggctcat ttcattgccg
    13981 ttttcatcaa aaaacagaat cgttggcaaa cctaatacat taaagtgttt catcagctcg
    14041 atattttcag ccgagttttt cgtcatatcg atttgcaaca ctaccatttc attcagtttg
    14101 ttctgaacat tttggtcact aaaggtgtat ttttcaaact ctttgcaggc gacacaccag
    14161 tcggcataga gatcgagcat cactttttta cctttggaag cggtcaattt tgtctctaaa
    14221 tcggcaagtg attgaatttt ttctaacgct aagtgcggtg tagtttgctg ttgagtggtt
    14281 gagccgttcc aagctaaatc tgcccaaggt ttagcggcaa tggctaaagc gagcaataat
    14341 aagactttga tgatcttttt cgtgattgaa ttagatggaa tcgcatccac caaccagatc
    14401 aaaaatgcaa ccgcaagggc cgaccataaa aacggttcat agtggcttgg cagcacacgg
    14461 cttaataaga acaccggtaa ggcaagcata acaaaaccga atgcagcttt cacttttaac
    14521 agccattcgc cggattttgg cagaattttg ttgccgaata aggtaatcaa aatcagcggt
    14581 aagcccattc ccagtgcgag tagatagagg gctaagccac cggtgactaa atcgccactt
    14641 tgtgccacat aaagcaacgc accggaaagt ggggcagaag tacaagggga agcaattaag
    14701 cctgcgatca tccccattaa aaacacgcta cccaacgcac caccttgctg tttttggctc
    14761 attgcattga gtttttgttg ccagctattt ggtagctgga tttcgtataa gccaaacatt
    14821 gagcaggcaa gcagaataaa gacgatagat aaaccaatca acaccggtgg gctttgtaag
    14881 gcgatttgga aaggcaagcc gatagccgct acaattaagc caagtaaggt gtaagtaact
    14941 gccattcctt gcacataggc aatacttaac agcaatgctt tagcggtaga agggcgttgc
    15001 ttatttccaa ttacaatcgc agagagaagt ggtagcatcg gcagtacgca aggagtgaat
    15061 gctaatccaa cgcctaatac aaaaaaccaa aaaatggaaa aacgattgtt agctaaattt
    15121 tcagccagtt gatcttgctc tgcttttgcc acattttcag ggggcgccat attgtcggtt
    15181 tttgcaaaat tttcgctctt ttcgaccgct tgtgcttcca gttgcgccag ttcttcaggt
    15241 gtagcagaga gaatactgtc taatttaagc tcaacccgtt ccggtggata gcaaaagcct
    15301 ttggtacacc cttgatattc cactacgatt gaggcgttgt ctttaatttc tttaaacggt
    15361 acctttagcc ctaagtggtt gcgatagatt tccacctgcc cgaaaaattc atcgttatat
    15421 tgctcggcag cgggaaaagt gatgtcgcct atctcaatat tctgtggcgt gacacggatt
    15481 tcttttttat aaagataata attgtctgca atatcccaat ttaactgtaa tgtttcattt
    15541 tcattttgga ttgaaaaaac aaaggcttct tccgatttta ggaatttggg ttgagagaa
//
LOCUS       DANLXF010000045        15439 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.045, whole genome shotgun sequence.
ACCESSION   DANLXF010000045 DANLXF010000000
VERSION     DANLXF010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 15439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15439
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.045"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..178
                     /locus_tag="PXD66_000839"
     CDS             <1..178
                     /locus_tag="PXD66_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319162.1"
                     /translation="FKEKGYDEFLAEKIRLGLEDMQSGNGLSLDESKARTKQLIERKA
                     RELANFEQENIIYG"
     gene            171..458
                     /locus_tag="PXD66_000840"
     CDS             171..458
                     /locus_tag="PXD66_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL6319163.1"
                     /translation="MAKTLRISSRAQNDIEEILASVIEYTGFESSGIRLQEDIYQKFE
                     TIAYMPSAAGRLREDGTREAFTRRYRIVYTELENEVWIITIIHSSRLYPRI"
     gene            complement(584..2686)
                     /locus_tag="PXD66_000841"
     CDS             complement(584..2686)
                     /locus_tag="PXD66_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HDL6319164.1"
                     /translation="MKSFKLLPVSAAVLSVLAANAVAQTASTDAVVLDTVTVTDNQGL
                     KVQSNVVTTKKKDESTQTDLRGLLKDEPAISLGGGNGTSQYLYIRGMGQNSIDVKVDN
                     TYSDSQILYHQGRHMLDPALVKIVSVQKGAGSASAGIGQTNGAVIAKTVDALDLLKNS
                     DKNFGAKLGTGFSTNHAHNFNATLYGKGEIFDALVSGNLVRDRDYKGGKDYVNQFGTN
                     RVPYSALDKTSFLAKLGATLGDHRFVLSHSSERNEGQRLVREEFDVAPNGTKVSPYNR
                     LTVERQAPANRRITVQRTNLEWTGKNLGFAQEATANVYQLVQGRWSENETGNGYAGGV
                     DYATKTKIVTHGANVNFDSALHENVLLKYGVNYRHQEAKPHTKFNPLVKNQEKTDTGV
                     YVEAITAPVDKVTLTTGVRYDHFKFKAMDGKERSNGAFSPSVGLIYEPIEHLSLSASH
                     NYATRSPRLYDALTAHGNRKIVSIGSNTKAEKARNTEIGFNYNNGTFSFDGSYFWQDI
                     KDALGGGRDSHNPNNELIMNLGKIKNRGYELNAGYHNGGFTARIGVAHSKPRFYSPTI
                     WEVNRQTGKREEKPLLSTNPEYASAIGRTWTASLSYRFEQPNLEVGVHHRIVEKVKPE
                     DNFFVTGGTLQTNTGTGKDGYNVTDITLNWKPLKDDSVNVNFAVDNVANKKYNAHGQR
                     GQLSARGREFRAGVNYTF"
     gene            2969..3715
                     /locus_tag="PXD66_000842"
     CDS             2969..3715
                     /locus_tag="PXD66_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL6319165.1"
                     /translation="MQLRHKQIMEYIQQFDEANVQELAEHCGVSIETIRRDLNKLDKD
                     GLLHRTHGGAVSHKKRDIGRSFTTRQRLNSEAKRSIAENVLEYLQPESVIALDASSTS
                     WNVAQRIPNIPCTVVSSSMRIIYSLSHKPHIKTIATGGIYSEKYDAFYGPLSEQLLTR
                     LKIDLAILSCTGIADGAIWESNEINISLKRKLIANSKQVFLLADHSKFERKDLFRIED
                     LSCIDKLFINQPPPDFLKNYCLAHQIEIII"
     gene            3911..5674
                     /gene="fucI"
                     /locus_tag="PXD66_000843"
     CDS             3911..5674
                     /gene="fucI"
                     /locus_tag="PXD66_000843"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005619331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HDL6319166.1"
                     /translation="MTTLKSTPVKIGIRPTIDGRRLGVRESLEDQTMNMAKAVAQLIQ
                     SEVRHPNGDFVECVIADSTIGGVAEAAACAEKFKRENVGAVITVTPCWCYGSETIDMD
                     PHMPKAIWGFNGTERPGAVYLAAALAGHSQLGLPAFSIYGTEVQEADDQSIPDDVREK
                     LLHFARAGLAVATLRGKSYLSIGSVSMGIAGSIVNQQFFQEYLGMRNEYVDMTEIKRR
                     LDRHIYDAEEFKLAMSWVKTYCKEGIDVNAPENQRSPEEREKLWEDVVKMTIIARDLM
                     VGNPRLAELGYSEEALGHNAIAAGFQGQRQWTDHLPNGDFMEATLNSTYDWNGVRAPY
                     IMATENDSLNGVNMLFGNLLTGQAQIFADVRTYWSEDSVERVTGWRPESGFIHLINSG
                     SAALDGTGQHTDRAGKPTIKPAWEVTEEDGKRCLENTCWCPAVHEYFRGGLSSQFLTK
                     GGMPFTMHRINLIKGIGPVLQIAEGWSIDLPENVHDILNKRANETWPTTWFVPRLTGK
                     GAFTDVYSVMANWGANHCVATFGHVGADLITLASMLRIPVCVHNVEDKDVFRPSAWNG
                     FGQDKEGQDYRACANFGPLYK"
     gene            5868..7301
                     /gene="fucK"
                     /locus_tag="PXD66_000844"
     CDS             5868..7301
                     /gene="fucK"
                     /locus_tag="PXD66_000844"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HDL6319167.1"
                     /translation="MPISLIFDCGATNLRTIAINQAGEIVAAHHLPNNTQPDVESPEF
                     HIWDIEEIWSKLMTCAKQTLSQLSEQQRQEIVGISVTTFGVDGALFDKNGKQLYPIIS
                     WKCPRTLPIMENLANYLDVEALYQRNGVGHYSFNTLFKLLWLKENKPEIYQQAESFLF
                     ISSILTYLLTGVQTTDRTMAGTSMMTNIENDSWDKTVLDLLGLTENHFPPMKSAGEVV
                     GTLKGEISQELGLSGQIPVISCGHDTQFAIFGSGAGYNQPVLSSGTWEILMVRTPKAK
                     PQWQFVQNGLTIEFDSQSGYFNPGVQWVASGVMEWFGKRFFSDIANTEHYYRTMIEEA
                     AEVPAGSNGVKLVGNFDGTTAKTGSIIGLSMHITRGEIYRAGLEYMAHRLKEGLDVLQ
                     EVGHFNAESLICVGGGSKNALWNQIRADVLNRPIDVVDFPESTVLGAAMFTFTGAGVF
                     ENPNAAQQAVKPNVKRVEPSSNSQFYK"
     gene            7376..7807
                     /gene="fucU"
                     /locus_tag="PXD66_000845"
     CDS             7376..7807
                     /gene="fucU"
                     /locus_tag="PXD66_000845"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012341412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HDL6319168.1"
                     /translation="MLKGIHPAISPDLLKVLAEMGHGDELVLSDAHFPAHSIHQNVIR
                     ADGLSVATLLEGISALFEFDQYVEAPLAMMQAVKGDTLDPSVEERYLNAIKKVNGTAP
                     KIERVERFAFYDRAKNAYAVVITGELAKYGNIIIKKGVTPV"
     gene            7867..8520
                     /locus_tag="PXD66_000846"
     CDS             7867..8520
                     /locus_tag="PXD66_000846"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HDL6319169.1"
                     /translation="MDRKILARQIIDTCLEMTRIGLNQGTAGNVSVRYKDGMLITPTG
                     TPYEQMTEDSIVFVDGNGKHEEGKLPSSEWQFHLAVYEARPELNAVVHNHAKNCAAVS
                     ILGEPIPAIHYMIACTGTNHIPCVPYAPFGTHQLADYVREGIKHSKAILLAHHGLITA
                     DKTLEKALGIAHEVEVIAEWYLKLLATGKPIPTLNKEQMDIVLGKFKSYGSWVESEQ"
     gene            8582..10111
                     /locus_tag="PXD66_000847"
     CDS             8582..10111
                     /locus_tag="PXD66_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL6319170.1"
                     /translation="MQNEVILTMKNISKSFAGVNALKGVELTLRKGEVHALMGENGAG
                     KSTLMKCLLGVYQADEGEIIYKGQPVRFESVLEAQKAGISMIFQELNLIPHLTVAENI
                     FFAREPLKHGLVDKDKMVEESAKLLNFFEIDVDPNDEVRMLSVAKQQMVEIAKALSFD
                     VEVLIMDEPTSALTEKEIDKLFELVDRLRAKGVSIVYISHRMEELKRICNHITIFRDG
                     TYVSNHKFNEITMDEIITKMVGRSLDNHFPPKTAVVTNDIILSIMNAERKGVFEPLNF
                     DLRKGEILGITGLVGAKRTELARAIFGADPLDSGEIFVHENKVSIKDPSDAIKAGIAY
                     LSEDRKLNGVAVRMSIRENITMASMDKVANQIGVISYDEEEKASKTFIDKMEIKTPTI
                     EQKVQNLSGGNQQKVVIGKWLFREAKVMIFDEPTRGIDVGAKYAIYQLLDELAANGVG
                     VIVISSELPEVLGVSDRIIVMREGRMTGMLETKKTNQEEIMHYATGVKNMFAREYGVE
                     K"
     gene            10108..11106
                     /locus_tag="PXD66_000848"
     CDS             10108..11106
                     /locus_tag="PXD66_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL6319171.1"
                     /translation="MNEKQKEMLRKMAALAGLVLLIIFFSVTNEYFFTSNNIMTVGLQ
                     TSTIALIGIGATCVILTGGIDLSTGSVVALSGVAAAMIVNAGVPVPLGMILGILVGGI
                     CGLINGILVTRMKLPPFIATLGMMMVARGLALYVTNAAPVSGMPESFANLGNGALFKI
                     VEEGANGLPKVVFAGIPYPVIIMIFITVLFTFALSKLKVGRYLYAIGSNEEAARLSGI
                     KTNIVKIYAYVASGLLSGLTGVILASRLVTAQPNGGVSYELDAIASAVVGGTSLMGGV
                     GTIPGTLIGSFIIGVLRNGLNMNGVSSFVQMIVIGLVIIVAVSLDQLRQSKKAGKK"
     gene            11130..12077
                     /locus_tag="PXD66_000849"
     CDS             11130..12077
                     /locus_tag="PXD66_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL6319172.1"
                     /translation="MKKSALFTATLFGLSTLFAGSAMAKSDEIAVIVKSANSTFWQNV
                     RKGAETAGKDIGGQYKVTFQGPEAETAIDAQVNMVDNAVNRGVAGIVLAASDPVALVP
                     AVKKAYESGIPVVLIDSGINSDGKYYQSFLATDNRAAGKLAAEKLLAKVKGGKVAVMS
                     FTQGAASAIERTGGFIDEIKAKADYKLVGPYYSDSDMTKALNQTEDVLGSNPDLAAIF
                     GANEPTAVGMARAVKQKGFAGKIVAVGFDGNSDLQNFVRDGTLDGIVVQSSYQMGYKG
                     VDTIGKLIKGEKVEKVIDTGVVYVTKENIDSEEAKGVLY"
     gene            12133..12384
                     /locus_tag="PXD66_000850"
     CDS             12133..12384
                     /locus_tag="PXD66_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HDL6319173.1"
                     /translation="MSNTLTDKALVVIAALGGRENIVDVYACITRLRVEVKNTDIVEW
                     DKIKSVGALGVVTSGSVIQSIFGSDAEAYRDEIKRILNI"
     gene            12513..13664
                     /gene="fucO"
                     /locus_tag="PXD66_000851"
     CDS             12513..13664
                     /gene="fucO"
                     /locus_tag="PXD66_000851"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HDL6319174.1"
                     /translation="MANRLILNETSYHGKGAIQNIVHEVTSRSFKKALVVTDKDLIKF
                     NVAQKVTALLDAAGLPYEIFDEVKANPAVDVIKAGVAKFKASSADYLIAIGGGSPIDS
                     AKAIGIIINNPEFSDVLSLEGVAPTHQKCVPIIAIPTTAGTAAEVTINYVITDEAKKR
                     KFVCVDVHDVPAVALVDPDMMESMPKSLAAATGMDALTHAIEGYITKAAWELTDALHL
                     KAIELIGRSLRGAVEKNPKDVEAMALGQYVAGMGFSNVGLGAVHSMAHPLSAYYDTPH
                     GIANAVLLPYVMEFNKNYTGEKYREIARAMGVKGVDEMSQEEYRNAAIAAVQQLSQDV
                     GIPPKLHQIGVKEADLAALSVDAFNDVCTGGNPRDCTAEELLEVYRIAY"
     gene            13931..15376
                     /gene="aldA"
                     /locus_tag="PXD66_000852"
     CDS             13931..15376
                     /gene="aldA"
                     /locus_tag="PXD66_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDL6319175.1"
                     /translation="MVKQHKMYINGEFVPALDGRTLAVYNPATEKAISEVPRGGADDA
                     KIAIDAAEAAQEAWERLPAIERGKYLRQIAVGIRERADEIARTISEEMGKTLSLATVE
                     VHFTADYLDYMAEWARRYEGEIIQSDRPNEHIFLYKKAIGVTTGILPWNFPFFLIARK
                     AAPALVTGNTIVIKPSEDAPNNAILFSEIVHQVGLPKGVFNVVTGYGNEVGPELSGNE
                     KIGMVSFTGSQFAGQKVMEAASKNIIKVNLELGGKAPAIVLDDADLELAATAVVNSRI
                     INSGQVCNCAERIYVQSSIKEQFVQKLVEKMQAVKYGNPLEQQDIDMGPMVNEKGLHK
                     VQEMVTHALHQGGNLVLGGNVVEGTGYYFEPTIIDNVDNSMDIMRHEIFGPVIPVATF
                     DTIDEAIRLANDCEYGLTSSVYTQNINKAMKVISRLKFGETYVNRENFEAMQGYHAGW
                     RKSGIGGADGKHGLEEYLQTQVVYLQFDDKV"
BASE COUNT         4631 a         3151 c         3388 g         4269 t
ORIGIN      
        1 gtttaaagag aaaggctatg atgaattcct agcagaaaaa attcgccttg gcttagaaga
       61 tatgcaatca ggtaatggtc tttcactcga tgaatcaaaa gcacgaacca aacaactgat
      121 tgaacgtaaa gcaagagaac tcgcaaactt tgaacaagag aatatcattt atggctaaaa
      181 cattacgaat tagcagtaga gctcaaaatg atattgaaga aattttagcg agcgtaattg
      241 aatatactgg ttttgaaagt agtggaattc gcttacaaga agatatttac caaaaatttg
      301 agactatcgc ttatatgccc tctgcggctg gacgtttaag agaagatggc acaagagaag
      361 cattcactag gcgttatcgt attgtttata ccgaattaga aaatgaagtg tggattatta
      421 ccattattca tagtagcaga ctttatccac gcatttaaat aacaaacgac taaaatagtc
      481 ggttttttat ttgcaaaaaa ttaataaaat atcaccgctt gtaagctcca taaaaaaaag
      541 cccgattgta acatcgggcg ttttagtctg aatttaagtc agattagaat gtgtagttca
      601 cacctgcacg gaattcacgt ccacgagctg ataattgacc acgctgaccg tgtgcgttgt
      661 attttttatt cgccacgtta tccactgcga agtttacatt cacgctatca tctttaagtg
      721 gtttccagtt taaggtgata tcagttacat tgtagccgtc tttgcctgta ccggtatttg
      781 tttgtaatgt tccgcccgta acgaagaaat tatcttcagg tttcactttc tcaacaatac
      841 ggtggtgtac gccaacctct aaatttggtt gttcaaagcg atagcttaaa gaagctgtcc
      901 aagtacgacc gatagctgag gcgtattctg ggttagtact taataatggt ttttcttccc
      961 gcttaccagt ttgtctgttt acttcccaaa tagttggtga gtagaaacgc ggtttgctgt
     1021 gtgcaacacc aatacgagca gtaaaaccac cattatggta acctgcattt aactcataac
     1081 cacggttttt aattttacct aggttcataa ttaactcatt attaggatta tggctatcac
     1141 gaccaccacc taatgcatct tttatatctt gccagaaata gctaccatca aaactgaatg
     1201 taccattgtt atagttgaaa ccgatttcag tattacgagc tttctccgct ttagtattgc
     1261 taccaatact aacgattttt ctatttccgt gagccgttag tgcatcgtat aaacgagggc
     1321 tacgagttgc atagttgtgg cttgcactta agcttaaatg ttcaattggc tcatagataa
     1381 gcccaacgct tgggctgaat gcaccattac tacgctcttt accatccatt gctttgaatt
     1441 tgaagtggtc gtaacgtaca ccggtagtta aagttacttt atctactgga gcagtaattg
     1501 cttcaacata cacgccggta tcggttttct cttggttttt cacaagaggg ttaaatttag
     1561 tgtgtggttt agcttcttgg tggcgatagt tcacaccgta ttttaataat acattttcgt
     1621 gaagggcaga atcaaagttt acgtttgcac cgtgggttac tattttagtt ttggttgcat
     1681 aatctacacc accagcataa ccattacctg tttcattctc agaccaacga ccttgtacta
     1741 actgatacac gttagctgtt gcttcttgag caaaacctaa gtttttacca gtccattcta
     1801 agttagttct ttgaaccgta attctacggt tagctggagc ttggcgttct acagttaagc
     1861 ggttgtacgg agaaaccttt gtgccgttag gtgctacatc aaattcttca cgcactaaac
     1921 gctgaccttc atttcgctca cttgaatggc ttaatacaaa acggtgatct cccaatgttg
     1981 cacctaattt agctaaaaag ctggttttat ctagtgcact atacggaaca cggtttgtgc
     2041 cgaattggtt tacgtaatct ttaccgcctt tatagtcgcg atctctcaca agattacctg
     2101 agactaaagc atcaaagatt tcgcctttac catataaggt tgcattgaaa ttgtgagcgt
     2161 ggtttgtgct gaagccagta cctaattttg caccgaagtt tttatcgctg ttttttaata
     2221 aatctaatgc atcaacggtt ttagcaatta ctgcaccatt agtttgacca ataccggctg
     2281 aagcactgcc tgcacctttt tgtacagaaa caattttcac caatgccgga tctaacatat
     2341 gacgaccttg gtgatacaaa atttggctat ctgagtaagt gttatctact tttacatcaa
     2401 ttgagttttg acccatacca cgaatataga gatattgtga tgtaccgtta ccaccaccta
     2461 agctaattgc cggctcatct tttaataaac cacgcaaatc ggtttgggtg ctttcatctt
     2521 ttttcttagt tgttaccaca ttgctttgta cttttaaacc ttgattatct gttacggtaa
     2581 cagtatctaa tactactgca tctgttgaag cagtttgagc cactgcatta gcagcaagta
     2641 cgctcaatac cgcagcagaa acagggagca atttgaagct tttcatttta tttatattcc
     2701 ttaggtaaat tatttaagag gattatctgt ttgatattaa aaataattct caacaccaaa
     2761 cgttcgcaaa ttttacagag ataaaaatga ttgtcaatta gtctgaaatg aaatttcaca
     2821 aaatatatta caaattttac aaaaagttcc ctaaatttaa ccgcttgcaa cacaaaatgt
     2881 gatcgccttc taaaaatcaa atttgcttgc tcgacagtaa cgctacaagc ggttaaattt
     2941 ggagaaaaat ttacaaaaag gatagattat gcagctgcga cacaaacaga taatggaata
     3001 tatacaacag tttgatgaag ctaacgttca agaattggcg gaacattgtg gggtttcgat
     3061 tgaaactatc cgccgagatt taaataagct agataaagat ggattattac atcgcactca
     3121 cggtggagct gtgagccata aaaagcgaga tattggtcgc tcatttacca cccgacaacg
     3181 gctgaacagt gaggcgaaac gcagtatcgc agaaaatgtg ttggaatatt tacaaccaga
     3241 atcggtgatt gctttagatg ccagctcaac ctcttggaat gtggcacagc gtattccaaa
     3301 tattccttgc accgttgtca gcagttcaat gcggattatc tacagccttt cacacaaacc
     3361 gcacattaaa acgattgcca ccggtggtat ttattccgaa aaatacgatg ccttctatgg
     3421 tccattatcc gagcagttac tgacccgttt aaaaattgat cttgccattc tttcttgtac
     3481 aggcattgcc gatggggcga tttgggaatc aaatgaaatt aatatttccc ttaaacgtaa
     3541 attaattgcg aacagtaaac aggttttttt attagccgat cacagtaaat ttgagcgtaa
     3601 agacttgttt cgtatcgaag atttatcttg cattgataaa ttatttatca accaaccccc
     3661 accggacttt ctcaaaaatt attgcttagc acatcaaatt gaaatcatta tttagtgatt
     3721 tctgcccgat tttattcaga aataaaacaa atcgggcatt cttttttcac tcatttttac
     3781 caaaatcccc cattttttgt gatcgcactc acaaaattta aactaaattc gcatttttct
     3841 tcataactca tattgcgtat gcataataaa ttcaatcgca aattttttta accaatcggg
     3901 agaactattt atgacaacgt tgaaatctac ccctgtaaaa attggtatcc gcccaaccat
     3961 tgacggtcgc cgtttaggag tgcgtgaatc acttgaagat caaacaatga atatggcaaa
     4021 agccgttgca caattaattc aatctgaagt ccgccaccca aatggtgatt tcgttgaatg
     4081 tgtgattgct gattccacca tcggtggtgt tgccgaagct gcggcttgtg cagaaaaatt
     4141 caaacgtgaa aatgtcggag cggtgattac cgtaacccct tgctggtgct acggttctga
     4201 aaccattgat atggacccac atatgccgaa agccatttgg ggctttaacg gaacagaacg
     4261 cccaggtgca gtttatttag ctgctgcttt agcaggtcat agccaattag gtttaccggc
     4321 attctccatt tatggcacag aagtacaaga agctgacgat caatctattc cagacgatgt
     4381 gcgtgaaaaa ttactccact ttgcccgtgc cggcttagca gtggcaacct tacgaggaaa
     4441 atcatattta tctatcggct ctgtttcaat gggtattgcc ggttctatcg taaaccaaca
     4501 attcttccaa gaatatttgg gtatgcgtaa cgaatatgtg gatatgaccg aaattaaacg
     4561 ccgtttagat cgccatattt acgatgccga agaatttaaa ttagcaatga gctgggtgaa
     4621 aacctattgc aaagaaggta ttgatgtcaa cgcaccggaa aatcaacgca gcccggaaga
     4681 gcgtgaaaaa ctctgggaag acgtggtgaa aatgaccatt attgcccgtg atttaatggt
     4741 gggtaatcct cgcttagcag agctaggcta tagtgaagaa gccttagggc ataatgcgat
     4801 tgctgccggt ttccaagggc aacgccaatg gacagaccat ttgccaaacg gcgattttat
     4861 ggaagctacc ttgaattcga cctatgactg gaacggcgtt cgcgcaccat acattatggc
     4921 aactgaaaac gacagcttaa atggcgtgaa tatgttgttc ggtaacttat taaccggtca
     4981 ggcacaaatc tttgccgatg tgcggacata ctggagcgaa gactcggtag aacgtgtcac
     5041 cggctggcgt ccggaaagcg gttttatcca cttaatcaac tccggctctg cggcgttaga
     5101 cggaacaggt cagcataccg atagagcagg caaaccaact attaaaccgg cttgggaagt
     5161 gactgaagaa gacggtaaac gttgtttaga aaacacctgt tggtgtccgg cagttcacga
     5221 atatttccgt ggtggtttat cttcccaatt cttaaccaaa ggtggaatgc cgtttacgat
     5281 gcaccgtatc aacctgatca aaggtatcgg gccggtttta caaattgccg aaggttggtc
     5341 tatcgacttg ccggaaaatg tacacgacat tttaaacaaa cgcgccaatg aaacctggcc
     5401 gaccacttgg ttcgtacctc gcttaaccgg aaaaggcgca tttaccgatg tgtatagcgt
     5461 aatggcgaac tggggtgcaa accactgtgt ggcaaccttc ggacacgttg gggcagactt
     5521 aattaccctc gcctcaatgc tccgcattcc ggtttgtgtg cacaatgtgg aagataaaga
     5581 cgtattccgc ccaagtgcgt ggaacggttt cggtcaagac aaagaaggtc aagactaccg
     5641 tgcttgtgct aacttcggac cattatataa ataatacaca atcaccctct ccccttgtgg
     5701 gagagggaca gccaagcaat tcgttcagaa ttgaatggca gggagagggc tacaagcggt
     5761 cgcttttctc tcaaaattta caaatccctc tctctgatta aattgctaaa cgcaatttaa
     5821 tctatctctc taaaagggag atattaggat aagtaaagga gataacaatg ccaatttctc
     5881 ttattttcga ctgtggtgcc accaacctcc gtacgattgc gattaatcaa gcgggtgaaa
     5941 ttgtggcagc ccatcatcta ccgaataaca cacaaccaga tgtggaaagc ccagaatttc
     6001 atatttggga tattgaagag atctggtcga aattaatgac ctgtgccaaa cagacgcttt
     6061 ctcaactttc agaacaacaa cggcaagaga ttgttgggat ttcagtaacc acctttggcg
     6121 tggacggtgc attatttgac aaaaacggca agcagcttta tccgatcatt tcttggaaat
     6181 gcccgagaac cctaccgatt atggaaaacc ttgccaatta tcttgatgtt gaggcgttat
     6241 atcaacgtaa tggcgttgga cattatagtt tcaacacctt attcaaatta ttgtggttaa
     6301 aagagaacaa accagaaatt tatcaacaag ctgagagttt cttattcatc tcatctattt
     6361 tgacttacct tcttacaggc gtgcaaacaa ccgaccgcac aatggcggga acgtcaatga
     6421 tgacaaacat cgaaaacgat tcgtgggata aaacagtttt agatttatta ggcttaaccg
     6481 aaaaccattt tcctccaatg aaaagtgcag gagaagttgt gggaacatta aaaggagaaa
     6541 tttcccaaga gttaggttta agcggtcaaa ttccagtaat ttcttgcgga cacgatactc
     6601 aatttgccat ttttggctca ggagctggct ataaccaacc tgtgttaagt tcaggcactt
     6661 gggaaatttt aatggttcgt accccaaaag caaaaccaca atggcaattc gttcaaaacg
     6721 gcttaaccat tgagtttgat agccaatctg gttatttcaa cccaggtgtg cagtgggtag
     6781 cttcaggtgt gatggagtgg tttggcaaac gtttcttctc tgatatagcc aatactgaac
     6841 attactaccg cacaatgatt gaagaagcgg cagaagtgcc tgctggctca aacggtgtga
     6901 aattagtcgg caattttgat ggcacaacag ctaaaacagg ttcaattatc ggcttatcta
     6961 tgcacatcac tcgtggcgaa atttaccgtg caggcttgga atatatggct catcgtctaa
     7021 aagaagggtt agatgtatta caagaagtcg gtcattttaa tgctgagagt ttgatttgtg
     7081 tgggaggtgg ttctaaaaac gctttatgga atcaaattcg agccgatgtc ttaaatagac
     7141 cgattgatgt agtggatttc cctgaaagta ccgtattagg cgcggcaatg tttactttta
     7201 caggtgctgg tgtatttgaa aatccaaatg cggctcaaca ggcagtgaag ccaaatgtga
     7261 aacgggttga gccatcgtca aatagtcaat tttataaata aagtttctcc cctctctctg
     7321 atttgaggtt cattcagaac atcaaatcag agagagggta aaaaaggagt tcaaaatgtt
     7381 aaaaggtatt cacccggcaa tttctccaga tttattaaaa gtcttagccg aaatggggca
     7441 cggcgatgaa ttagtgcttt ctgatgcaca cttccctgct cactctattc atcaaaacgt
     7501 cattcgtgca gatggcttaa gtgttgccac cttattagaa ggcatttctg ctctgtttga
     7561 atttgaccaa tacgttgaag ccccacttgc aatgatgcaa gcagtaaaag gcgacacgct
     7621 cgatccaagc gttgaagagc gttacctcaa tgcaattaaa aaagtaaacg gaacagcccc
     7681 gaaaatagag cgtgtagaac gcttcgcttt ctatgataga gccaaaaacg cttatgccgt
     7741 tgtcattaca ggcgagttag ccaaatacgg caacattatt atcaaaaaag gcgtaacacc
     7801 ggtttaatta caagcggtta aaaaagcaga attttttaca aatataaaat taaaaggagt
     7861 tcaacaatgg acagaaaaat actggcgaga caaatcattg atacttgtct tgaaatgacc
     7921 cgaattgggt taaatcaagg tacagcaggc aatgtgagcg tacgttataa agacggtatg
     7981 ctcattacgc caacaggtac accttatgag caaatgaccg aagattctat cgtctttgtt
     8041 gatggcaacg gcaaacacga agagggaaaa ctcccttcaa gtgagtggca gttccattta
     8101 gcggtttatg aggctcgccc tgaattaaat gcggtagttc acaaccacgc taaaaactgt
     8161 gccgcagtgt ctattttagg tgaacctatc cctgccattc actatatgat cgcctgcacc
     8221 ggcactaatc acattccttg tgtaccttat gccccgttcg gcactcacca attagccgac
     8281 tatgtacgtg aaggcattaa acacagcaaa gcaattttac tcgctcacca cggcttaatc
     8341 accgcagata aaaccctcga aaaagccctc ggcatcgccc acgaggtgga agtgatcgca
     8401 gaatggtatt tgaaactgct cgctacagga aaacctatcc caacgctaaa caaagaacaa
     8461 atggatatcg tactcggaaa atttaagtcc tatggttctt gggtggaaag cgaacaatag
     8521 cctgccataa ataacataca agcggtgata tttcttaaaa tttttacaaa cggagtgcag
     8581 tatgcaaaat gaagtaatac tcacgatgaa aaacatcagt aaatcctttg cgggggtcaa
     8641 tgcgttaaaa ggtgttgagc ttacgctgcg taagggcgaa gttcacgcat taatgggcga
     8701 aaacggtgca ggtaaatcca ccttgatgaa atgcttgctg ggcgtatatc aagccgatga
     8761 aggtgaaatc atatataaag gtcagcctgt tcgctttgag tcggtattag aggcacaaaa
     8821 agcgggcatt agtatgattt tccaagaact gaacctcatt ccccatttaa cggtggcaga
     8881 gaatattttc ttcgcccgtg agccactcaa acacggttta gtggataaag acaaaatggt
     8941 ggaagaatcg gctaaattgc tgaatttctt tgagattgat gttgatccaa acgatgaagt
     9001 gagaatgttg tcggtagcca aacaacagat ggtagaaatt gccaaagccc tttcatttga
     9061 tgtggaagta ctgattatgg acgagccgac ctccgctctt accgaaaaag agatcgataa
     9121 attatttgaa ttggtcgata gactgcgtgc caaaggggtg agcattgtct atatttctca
     9181 ccgaatggaa gaattaaaac gtatctgtaa ccatattacg attttccgtg atggcaccta
     9241 tgtatctaac cataaattca atgaaatcac gatggatgag atcattacca aaatggttgg
     9301 gcgttcattg gataaccatt tcccaccaaa aaccgctgtg gtgacaaacg acattatcct
     9361 ctcgattatg aatgctgaac gtaagggagt gtttgaacca ttgaactttg acttacgcaa
     9421 aggagaaatt ctgggcatta ccggcttggt tggagcaaaa cgtactgaac tggctcgagc
     9481 catcttcgga gcagacccac ttgatagcgg tgagattttt gtgcacgaga acaaagtcag
     9541 cattaaagat ccgagtgatg caattaaagc cggtatagcc tatttgtcgg aagaccgtaa
     9601 gctcaatggt gttgcagtaa gaatgtcgat tcgtgagaat atcacgatgg catctatgga
     9661 taaagttgcc aatcaaatag gtgtgatttc ttacgatgaa gaagaaaaag cctcaaaaac
     9721 tttcatcgac aaaatggaaa tcaaaacccc aacgattgag cagaaagttc aaaacttaag
     9781 tggtggtaac caacaaaaag tggtaattgg taaatggtta ttccgtgaag caaaagtgat
     9841 gatttttgat gagccgactc ggggaattga tgtcggagca aaatatgcta tctatcaact
     9901 gctcgatgag cttgctgcca atggcgtggg tgttatcgtg atctcttccg aattgcctga
     9961 agtcttggga gtttccgacc gcatcattgt aatgcgtgaa ggtagaatga caggtatgct
    10021 cgagactaaa aaaaccaacc aagaagaaat tatgcattat gcaactggtg ttaaaaatat
    10081 gtttgctcgt gaatatgggg tggaaaaatg aatgaaaaac aaaaagaaat gttaagaaaa
    10141 atggctgcat tggctggctt agtactatta atcatctttt tcagcgtaac caatgaatac
    10201 ttctttacct ccaataacat tatgacagta gggctacaaa cctctaccat tgcgttaatt
    10261 ggtattgggg caacctgtgt gatcttaacc ggtggaattg atttaagtac cggttcagta
    10321 gttgcactct ccggtgtggc agctgcaatg attgtaaatg ccggtgtacc cgtaccacta
    10381 ggaatgattt tagggatttt ggtcggcggt atttgtggct taatcaacgg cattttagtc
    10441 accagaatga aattgcctcc gtttattgct accctcggta tgatgatggt tgcccgtggt
    10501 ttagcattgt atgttaccaa tgccgcacca gtttccggaa tgcctgaatc ctttgccaat
    10561 ctcggtaacg gagcattatt taaaattgtg gaagaagggg caaacggctt gccgaaagtg
    10621 gtatttgcag gtattcctta ccctgtgatt attatgatct ttattaccgt actattcact
    10681 ttcgctctct ctaaactgaa agtcggacgc tatctttacg caattggttc taacgaagaa
    10741 gccgcacgct tatccggcat taaaaccaat attgtcaaaa tctatgccta tgttgccagt
    10801 ggcttactct ctggtttaac tggcgttatc cttgcctccc gcctcgtcac cgcccagcca
    10861 aacggtgggg tctcttacga gctggatgcg attgccagtg ctgtggtggg cggcacatcg
    10921 cttatgggcg gtgtgggtac tatccccggc acactcatcg ggtcattcat tatcggggta
    10981 ctccgtaatg gtttgaatat gaatggcgtt tcttcatttg ttcaaatgat tgtcatcggg
    11041 cttgtgatta tcgtggcggt ctctctcgac cagctccgcc aatccaaaaa agccggtaaa
    11101 aaataatcca ctcttaaagg agaattaaaa tgaaaaaatc agcattattt actgcgaccc
    11161 tctttggttt atcgacctta tttgcaggca gtgcaatggc aaaatctgat gaaatcgctg
    11221 tgattgtgaa atctgccaac tctaccttct ggcaaaacgt gcgtaaaggg gctgaaactg
    11281 ccggcaaaga tattggagga caatacaaag tcaccttcca agggccggaa gccgaaacag
    11341 cgattgacgc ccaagtgaat atggtggaca acgccgtaaa ccgtggcgtg gcaggcatcg
    11401 tactggctgc ctcagacccg gtagcattag tgccggcggt gaaaaaagct tatgagagtg
    11461 gcattccggt ggtgcttatc gattccggca tcaacagtga cggcaaatac taccaatctt
    11521 tccttgccac cgacaaccgt gcagcaggta aattagcagc ggaaaaactg cttgccaaag
    11581 tcaaaggcgg caaagtggca gtgatgagct ttacccaagg ggcagcatca gcgattgagc
    11641 gtaccggtgg ttttattgac gaaatcaaag caaaagccga ttacaaactg gtcggcccgt
    11701 actactccga ttcggatatg accaaagcgt taaaccaaac cgaagacgta ttaggttcta
    11761 accctgatct tgcagcgatc ttcggggcga acgaaccgac tgcggtagga atggcacgag
    11821 cggtgaaaca aaaaggcttt gccggcaaaa ttgtagcggt gggctttgat ggaaactccg
    11881 acctacaaaa cttcgtgcgt gacggaacac tagacggcat cgtggtgcaa tcttcctacc
    11941 aaatgggcta taaaggcgtg gacaccatcg gcaagctgat taaaggcgag aaagtcgaaa
    12001 aagtgattga taccggggtg gtttatgtca cgaaagaaaa cattgatagc gaagaagcga
    12061 aaggtgttct ttactaacat tcaagttaaa cggggtgggg ttgcccaccc tttcagccga
    12121 aaggaagcca atatgtcaaa tacccttacc gataaagcct tagtggtgat tgccgcactt
    12181 ggcggtcgag aaaatattgt cgatgtttac gcctgtatta cccgcctacg tgtggaggtg
    12241 aaaaataccg acattgtgga atgggataaa attaaatctg tcggtgcgtt aggcgttgtg
    12301 acctcaggct cagttattca atctattttt ggtagcgatg ccgaagccta ccgtgatgaa
    12361 atcaaacgta ttttgaatat ttagcctaag cacactcaca acaaaagaat cgcaacaagc
    12421 ggtgaaattt ttatcaaaat ttgcaaaaaa atgacgtaat ttgaccgctt gcaagttaat
    12481 cgaattgttt aacttttaag aaggaaaata gtatggcaaa tcgtcttatc ctcaatgaaa
    12541 ccagttacca cggcaaagga gcaatccaaa atattgttca cgaagtgacc agccggagtt
    12601 tcaaaaaagc ccttgtggta acagacaaag acctgatcaa atttaacgtt gcccaaaaag
    12661 tcaccgcctt gcttgatgcc gcaggcttgc cttatgagat tttcgatgag gtcaaagcca
    12721 acccggcagt cgatgtgatc aaagcggggg tcgcaaaatt caaagcctcc agtgccgatt
    12781 atttaattgc gattggtggc ggctcaccga ttgatagtgc caaagcgatt ggtattatca
    12841 tcaataaccc tgagtttagc gatgtacttt cattggaagg tgtcgcccca acccaccaaa
    12901 aatgtgtgcc gattatcgcc attccaacta ccgcaggtac agccgcagaa gtcacgatta
    12961 actatgtcat taccgatgaa gcgaaaaaac gcaaatttgt gtgcgtggac gttcacgatg
    13021 tgccggcagt ggcgttagtc gatccagata tgatggaatc tatgccgaaa agcttggctg
    13081 ccgcaaccgg tatggacgct ctcacccacg ccattgaagg ctacattacc aaagcggcgt
    13141 gggaattaac cgatgctcta caccttaaag cgattgagct gatcggccgt tcattgcgtg
    13201 gtgcagtgga gaaaaatccg aaagatgtcg aggcaatggc attgggacaa tatgtggctg
    13261 gaatgggctt ctccaacgtt gggcttggcg cggttcacag tatggcacac ccgctttctg
    13321 cctattacga caccccacac ggcattgcca acgcagtatt actgccgtat gtgatggaat
    13381 ttaataaaaa ttacaccggc gaaaaatacc gtgaaattgc ccgagcaatg ggggtaaaag
    13441 gcgtagatga aatgagccaa gaagagtacc gcaacgccgc cattgctgcc gtacaacagc
    13501 tttcacaaga tgtcggcatt ccgccaaaac tgcatcaaat cggcgttaaa gaagccgatt
    13561 tagcggcatt atcggtcgat gcctttaatg atgtctgcac cggtggcaac ccgagagatt
    13621 gtacggctga ggagctgttg gaggtatatc ggatcgcata ctaagttcta tctttgttaa
    13681 atagacaagc ggtgattttt taataaaaat ttgcaatttc tgttaaaaaa atactgatag
    13741 tcataatgcc cgatttctca tatttatgga aaaatcgggc attttcgttg cttttttatc
    13801 gccaagaaat gtgattttgt tcacatttat atctataaat taacaaaaat aaaacaattt
    13861 gtttgcaaag gcggttcagt taccgaatga tagtcatcaa cttccactgt ggataaaaaa
    13921 ggagcaactc atggtgaaac aacataaaat gtatattaat ggtgaatttg tgcctgcgtt
    13981 agatggtcgc actttagcag tctataaccc tgccaccgaa aaagctatct ccgaagtccc
    14041 tcgtggcgga gctgatgatg ccaaaatcgc tatcgatgct gctgaagccg cccaagaagc
    14101 ttgggaacgt ttaccagcga ttgaacgagg aaaatattta cgtcaaattg ctgtggggat
    14161 tcgtgagcgt gccgatgaaa ttgctcgcac tatttccgaa gaaatgggta aaactctgtc
    14221 cttagcaacc gttgaggtac attttaccgc tgactactta gactatatgg cagaatgggc
    14281 gagacgttat gaaggggaaa ttatccaaag tgatcgccca aatgaacata ttttcctata
    14341 caaaaaagcg attggcgtca ccaccggcat tttaccttgg aacttcccgt tcttcctcat
    14401 tgcccgtaaa gcggctccgg cattagtaac aggtaatact attgtgatta aaccgagtga
    14461 agatgcacct aataatgcga ttctcttttc ggaaatcgtg catcaagtcg gtttacctaa
    14521 gggggtattt aatgtcgtca ccggctatgg caatgaagtc gggccggaac tttcaggcaa
    14581 tgaaaaaatc ggtatggttt cctttaccgg tagccagttt gccggacaaa aagtgatgga
    14641 agccgccagc aagaatatca ttaaagtcaa tttggaattg ggcggaaaag caccggctat
    14701 cgtattagac gatgcagatc ttgagcttgc tgctaccgct gtagtaaatt ctcgaatcat
    14761 caattcagga caggtttgta actgtgcaga acgcatttat gtacagagtt caattaaaga
    14821 gcaatttgtc caaaaattgg tagagaaaat gcaagcggtg aaatatggta atccattaga
    14881 acaacaagac attgatatgg ggccgatggt aaatgaaaaa ggcttgcata aagtgcagga
    14941 aatggtgaca cacgcactgc atcaaggagg taatctggta ttaggtggta acgtggttga
    15001 aggaacaggc tactactttg agccaactat tatcgacaat gtagataact ctatggatat
    15061 tatgcgtcac gaaatttttg gtccggtcat tccggttgcg acatttgata ccattgatga
    15121 agcgattcgc ttagccaatg attgtgaata tggcttaacc tcttcggttt acacgcaaaa
    15181 tatcaataaa gcgatgaaag taatttcccg cttaaaattc ggagaaactt atgtaaaccg
    15241 tgaaaacttt gaagcaatgc aaggctacca cgccggttgg cgtaaatcgg gtattggcgg
    15301 tgctgacggt aagcacggct tagaggaata cctacaaact caggtggttt atctgcaatt
    15361 tgatgataaa gtatagattc ttctaaaaac tcccacttat caaaataagc gggagttttc
    15421 ttttaggtta accttgtat
//
LOCUS       DANLXF010000046        15126 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.046, whole genome shotgun sequence.
ACCESSION   DANLXF010000046 DANLXF010000000
VERSION     DANLXF010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 15126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15126
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.046"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            3..218
                     /locus_tag="PXD66_000853"
     CDS             3..218
                     /locus_tag="PXD66_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HDL6319176.1"
                     /translation="MFPEFRDLITQLKQNNSHFARLFDKHNELDQRIKNMEANIELAT
                     HEEVEALKKEKLHLKDQLYAILKQESA"
     gene            complement(277..1434)
                     /locus_tag="PXD66_000854"
     CDS             complement(277..1434)
                     /locus_tag="PXD66_000854"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HDL6319177.1"
                     /translation="MSLDLADIRTQITNLDRNLLKLLAERHRLAFDVVRSKEITQKPL
                     RDLEREKILLQELVNFAEAENYQLDPQYVTQIFQRIIEDSVLTQQNYLQNKLNAQKEE
                     QVSIAFLGMRGSYSNMASRQFAKKYQGTLAELSCHSFNEVFEKVSLGEADFGVLPLEN
                     TTSGSINEVYDLLQHTDLTLVGELAYPIKHCVLANGTVDLAEVDTIYTHPQPAQQCSQ
                     FLAGLDKVHIKYCESSSHAMQMVARLNKPNIVALGNEDGGKLYGLTNIKTDIANQAHN
                     ITRFIVVAKEAVQVSPQVQTKTLLLMTTTQQAGALVDALMVFKNHHIRMMKLESRPIY
                     GKPWEEMFYVELEANIHSENTQKALAELEQVTSYLKVLGCYPSEIIEPVKL"
     gene            complement(1556..3622)
                     /gene="prlC"
                     /locus_tag="PXD66_000855"
     CDS             complement(1556..3622)
                     /gene="prlC"
                     /locus_tag="PXD66_000855"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HDL6319178.1"
                     /translation="MSNPLLNYTGLPAFSQIKPEHIKPAVEAVIKECRETVEKVAQIE
                     NPTWENFYMPQAMAEDKFSRAWSPVGHLNAVKNSPELREAYQACLPLLSEYSTWAGQH
                     QGLYQGYVKLKNSPEFETYSVAQKKAIENSLQDFELSGISLPAEKQKRYGEISARLSE
                     LNSQFSNNVLDATMGWDIVITDEAQLKGLPESALEAAKLSAESKGKKGYRFTLEIPSY
                     LPVMTYCENKALREEMYRAYVTRASDQGPNAGKWDNSAVIDEILKLRHEKAQLLGFKT
                     YSDYSLATKMAENPQQVLDFLNDLAARSKAQGKNELCDLKKFAKAHFGIEHLDLWDIP
                     FYSEKQKQALYSVNDEELRPYFPENRVLSGLFEVIKRIFGMRVEEQKDVDVWHKDVRF
                     FNIFDKNDRLRGSFYLDLYARENKRGGAWMDDCINQKRLADGTLQKPVAYLTCNFNKP
                     IGDKPALFTHDEVTTLFHEFGHGIHHMLTEIDVGDVAGINGVPWDAVELPSQFLENWC
                     WEEEALAFISGHYETGEPLPKAKLEQLLAAKNFQAAMFVLRQLEFGLFDFRLHTEYDP
                     SKQGQVLALLKEVKDQVAVAKGVEWARTPHSFSHIFAGGYAAGYYSYLWAEVLSADAY
                     SRFEEEGIFNPETGQSFLDNILTRGGSEEPMVLFERFRGRKPTLDALLRHKGIVGNAE
                     PVDCTQ"
     gene            complement(3753..4493)
                     /gene="pflA"
                     /locus_tag="PXD66_000856"
     CDS             complement(3753..4493)
                     /gene="pflA"
                     /locus_tag="PXD66_000856"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HDL6319179.1"
                     /translation="MSVVGRIHSFESCGTVDGPGIRFILFLQGCLMRCKYCHNRDTWD
                     LDGGKEITVEELMKEVTTYKHFMKATGGGVTASGGEAVLQMEFVRDWFRACKAEGINT
                     CLDTNGFVRHYSPVVDEMLEVTDLVLLDLKQLNDEVHQDLVGVSNKRTLDFARYLHKL
                     NKPTWVRYVVVPGYTDDDDSAHRLGQFIQGMDNIEKVELLPYHRLGAHKWETLGYKYE
                     LEGVMSPPKEDLERIQKIIESYGHTVKF"
     gene            complement(4742..5767)
                     /locus_tag="PXD66_000857"
     CDS             complement(4742..5767)
                     /locus_tag="PXD66_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HDL6319180.1"
                     /translation="MSKQDLIRSSVAEYLTFITATGESQVDAVYADENVWLSQKMMAV
                     LYDVDVRTISYHLKKIFSDNELAENSVIQFFWITAQDGKNYHTKHYNLSAIIAVGYKV
                     NSERAVQFRKWATEIVHSFAIKGFAMDDERLKNDGTILGKKYFEEQLARIREIRLSER
                     KFYQKITDIYATAVDYDRTATATKRFFATVQNKLHWAIHGHTAAELIMARANAEKPNM
                     GLTNWKDAPQGKIYPFDVVVAKNYLSDNELAQLQRLVSAYLDMAEDMAERQIPMTMAD
                     WETRLNRFLAATDREILQDAGKVTAEIAKSFALSEFEKYRVKQDLTYESDFDLLVKEV
                     AAKYHAG"
     gene            complement(6002..8326)
                     /gene="pflB"
                     /locus_tag="PXD66_000858"
     CDS             complement(6002..8326)
                     /gene="pflB"
                     /locus_tag="PXD66_000858"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDL6319181.1"
                     /translation="MTQLTDAQQKAWEGFAAGEWQTEVNVRDFIQKNYTPYEGDESFL
                     AEVTPATSKLWADVMEMIKVENKTHEPYDIDCDTPSTITSHAPGYIDKSLEKIVGLQT
                     DAPLKRAIMPFGGINMVKGSCKVYRRELKPEVEQIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVITGLPDAYGRGRIIGDYRRMALYGADFLMKDKQRQFASLQARLESGEDIQATI
                     QLREEIAEQHRALGKMKEMAASYGFDISGPATNAQEAVQWTYFAYLAAVKSQNGAAMS
                     FGRVSSFLDIYIERDLKAGKITEQEAQELIDHLVMKLRMVRFLRTPEYDQLFSGDPMW
                     ATETLAGMGLDGRTLVTKNSFRILHTLYTMGPSPEPNLTILWSEQLPEAFKRYCAKVS
                     IDTSSVQYENDDLMRPDFQNDDYAIACCVSPMIVGKMMQFFGARANLAKTLLYAINGG
                     VDEKSGDQVGPKTEAITSEYLDYDDVMTRLDSFMDWLATQYVTALNIIHFMHDKYAYE
                     AALMALHDRDVFRTMACGIAGLSVAADSLSAIKYAKVKPIRTDIEIKNKAGEVVGVAK
                     NVATDFEIEGEYPQFGNNDNRVDDIAVDLVERFMKKIQKLKTYRNATPTQSVLTITSN
                     VVYGKKTGNTPDGRRSGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTF
                     SIVPNALGKDYEAQKRNLAGLMDGYFHHEATVEGGQHLNVNVLNRDTLLDAVEHPEKY
                     PQLTIRVSGYAVRFNSLTKEQQMDVITRTFTESM"
     gene            complement(8416..9240)
                     /gene="focA"
                     /locus_tag="PXD66_000859"
     CDS             complement(8416..9240)
                     /gene="focA"
                     /locus_tag="PXD66_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HDL6319182.1"
                     /translation="MFSPAEMAKIAEDGAVYKATKNQLYSFLSAITAGGFIAIAFVFY
                     TTTQVGASQMPWGMAKLIGGLVFSLGVIMCVVFGAELFTSSTLTIVARASQRINWLQM
                     FKNWVVVYFGNFVGAISIVLLIWFSGQIMAAKGQWGLVILNTAQHKIHHQWFEAFCLG
                     IFCNIMVCIAVWMSYAGKSLTDKAFIMMLPIAMFVASGFEHCVANMFMIPMGIAISHF
                     ASPEFWTAINVDPTQFADLDVSHFVFKNLIPATLGNIVGGVFFIGLMQWFLYIRKH"
     gene            9441..9935
                     /locus_tag="PXD66_000860"
     CDS             9441..9935
                     /locus_tag="PXD66_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDL6319183.1"
                     /translation="MTEYLTSFFSLFFSEQNQLFTMFLSGFLSATLLPGNSEIIFTTL
                     ASQHLITYGSLSSFSLFNLLLVATLGNSLGSITTYAIGLLLPRPQNTTNRYSQWALAK
                     SEKYGVLVLLFSWLPIVGDIFCGIAGWLRFSLWQSILFIVIGKLLRYLFLLATLYSVL
                     PHIL"
     gene            complement(9989..10858)
                     /gene="htpX"
                     /locus_tag="PXD66_000861"
     CDS             complement(9989..10858)
                     /gene="htpX"
                     /locus_tag="PXD66_000861"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HDL6319184.1"
                     /translation="MAKRVILFLLTNLAITFVLGIVLNLIFKVTGIQGQSTAGILVLS
                     LVFGFSGSLISLFMSKSMALRSVGAEVITQPRNEAEQWLFNTVQRQAQQANIPMPDIA
                     IYHSADVNAFATGATKSNSLVAVSTGLLNSMTKDEAEAVVAHEVAHIANGDMVTMTLL
                     QGVLNTFVIFLARIISTMAASSRDENGNSSTNTLVFWVVDIVLQMVFGVLATMIAMWF
                     SRQREFRADAGSAQLVGKEKMIAALQRLQRVHEPQEMEGSLAAFMINGSRGKELFMSH
                     PPLEKRIEALRNL"
     gene            complement(10946..12028)
                     /gene="dinB"
                     /locus_tag="PXD66_000862"
     CDS             complement(10946..12028)
                     /gene="dinB"
                     /locus_tag="PXD66_000862"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDL6319185.1"
                     /translation="MSIQAKHKRKIIHIDMDCFYAAVEMRENPALVGKPVAVGGNANQ
                     RGVLTTCNYEARKFGLHSAMPTATALKKCPNLILLPVNMALYKSVSAQIHEIFHRYTD
                     VIEALSLDEAYLDVTECERYFGSATWIAQAIRNDIFNELHLTASAGVAPLKFLAKITS
                     DQNKPNGQCVITPDDVQNFVKNLPLAKIPGVGKVTNEKLKQLGFETCGDIQQAEKAVI
                     YRAFGKFGQRLWDFSHGIDERAVEPYRERKSLAVENTLLHDISTLEQAEKIVADLFDK
                     LVFRLQRNWGDTPLTEFKKLGIKLKFDDFSQTTLERTTDGFAYERFGELLQQIFTRRK
                     ERKVRLIGLSVHFPEKKVNKQLNLWE"
     gene            12151..13062
                     /locus_tag="PXD66_000863"
     CDS             12151..13062
                     /locus_tag="PXD66_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HDL6319186.1"
                     /translation="MLYGLAIVLIPLFLGYLITLKNHRHMAIVNRVVSLCLYLILLVM
                     GISLGQIDNILTELPQIGGTAFSLVVILLLSNIIGLVIYDKLQPMKRDKVNQDQLPSR
                     LHLLIDSFKLIGITLLGGIIGYFTKGVVDFPLHASTYVLEVMIFGVGIQLRNSGIPLR
                     EVFFNKRGIYTSIVMVASSLIGGVISAFTLDLSLAKGLTFASAFGWYSLSSVLVNDAW
                     GPIYGSIAFFNDISREILCLFLVPLFMRQFPSTAVGIGGATSLDCMLPIIQKSGGIQI
                     VPLAISFGFIVNLAAPLLLAVFIGLEG"
     gene            13168..14934
                     /gene="aspS"
                     /locus_tag="PXD66_000864"
     CDS             13168..14934
                     /gene="aspS"
                     /locus_tag="PXD66_000864"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDL6319187.1"
                     /translation="MMRSHYCGALNRSHLGQTVTLSGWVHRVRNLGRFIFMQIRDREG
                     IVQVFFDEKDEALFKQASALRSEACVQIQGEVIARDESQINKEMATGEIEVLVKNLVV
                     YNNAEVLPLDFNQNNTEEQRLKYRYLDLRRPEMAEKLKTRAKITSFVRRFMDDNGFLD
                     IETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFR
                     DEDLRADRQPEFTQIDVETSFMTAEEVRALMEAMIHGLWKETLGVDLGKFPMMTWNEA
                     MTRFGSDKPDLRNPLEMVDVADILKSVDFKVFSGPANDPKGRVVALRVPNGAALTRKN
                     IDDYTQFVGIYGAKGLAWLKVNDMNTGLEGIQSPVAKFLNEEVLKALVKRLNLQKDDI
                     VFFGADNWHVATNAIGALRLKVGNDLGLTDTTAWKPLWVIDFPMFERDDEGNLSAMHH
                     PFTSPKELSPEELVQDPENAVANAYDMVINGYEVGGGSVRIFSQQMQQTVFSILGINE
                     EEQKEKFGFLLDALKFGTPPHAGLAFGLDRLTMLITGTDNIRDVIAFPKTTAAACLMT
                     EAPSYANPKALEELAIRTIPQE"
BASE COUNT         4271 a         3359 c         2993 g         4503 t
ORIGIN      
        1 atatgtttcc agaatttcgt gatctcataa cccaattaaa gcaaaacaat tcacattttg
       61 ctcgcttatt tgataaacat aatgagctag accagcgtat taagaatatg gaggcaaata
      121 ttgaactggc aacacatgaa gaagttgagg cactaaaaaa agaaaaattg catttaaaag
      181 accaacttta tgcaattcta aaacaagaat cggcttaacc taaagtaaaa gctcagaaaa
      241 tattctgagc tttaatttcg gtcatattag acaatattac agttttaccg gctcaataat
      301 ttcgcttggg tagcaaccca acactttgag gtagctggta acttgctcca attccgccaa
      361 tgctttttgg gtgttctcgg aatgaatatt tgcctccagc tccacataaa acatctcttc
      421 ccaaggtttg ccgtaaatcg ggcgggattc cagtttcatc atacgaatat gatgattttt
      481 gaataccatt aacgcatcga ccaatgcacc cgcctgttgc gtggtggtca ttaacagtaa
      541 cgttttggtt tgtacctgtg gcgaaacctg cacagcctct ttggcaacaa caataaaacg
      601 ggtgatattg tgagcttgat tagcaatatc ggttttgata ttcgtcaagc catacaattt
      661 tccgccatct tcattgccca aggcaacaat attcggctta ttcaagcgag ccaccatttg
      721 cattgcgtgc gagctgcttt cgcaatattt gatatgtact ttatctagcc cggctaaaaa
      781 ttggctacat tgctgggcag gctgagggtg ggtgtaaatg gtatccacct ccgccaaatc
      841 aaccgtgccg tttgccaata cacaatgttt gattgggtac gccaattcac ccactaaggt
      901 taaatcggta tgctgcaata aatcatacac ttcattgatc gaacctgaag tcgtgttttc
      961 aagcggcagt acgccaaaat cggcttcacc aaggctgact ttctcaaata cttcattaaa
     1021 agaatggcag cttaattccg ccaatgtgcc ttgatatttt ttggcaaact gtcgagatgc
     1081 catattagaa taagagcctc gcattcccaa aaacgcaatc gaaacctgct cttctttttg
     1141 tgcattcaat ttattttgca gataattttg ctgagtcagc actgaatctt caataatacg
     1201 ttggaaaatt tgggtcacat attgcggatc aagctggtaa ttctccgctt cggcaaaatt
     1261 taccagttct tgaagcaaaa tcttttcccg ttctaaatct ctcaagggtt tttgtgtgat
     1321 ttctttgctc cgcaccacat caaaggctaa gcggtggcgt tctgccagta gctttaataa
     1381 attgcggtct aagttggtta tttgggttcg aatatcggct aaatctaaag acattatttc
     1441 actctctgtc aggctaaatg ctgaaaatta taacgagttg caaaagaaag aaaagacaag
     1501 cggtcaaatt tacaaaaaaa tttgcaaatc taaccgcttg taaaacgtgt ttactttatt
     1561 gagtgcaatc aacaggctcg gcattaccta caatgccttt gtgtctgagc aaggcatcaa
     1621 gggttggctt acgcccacgg aagcgttcga acagcaccat tggctcttct gaaccgcctc
     1681 gggttaagat attatctaag aaagactgcc ccgtttcagg gttgaaaatc ccctcttcct
     1741 caaagcgtga ataggcatct gctgaaagca cttccgccca taaataactg tagtagcccg
     1801 ccgcatagcc gcctgcaaaa atatgcgaga aactgtgtgg cgttctcgcc cactctacgc
     1861 ctttggcaac agcgacttgg tctttcacct ctttaagtaa ggctaacact tgtccttgtt
     1921 tgctcgggtc atattcggtg tgtaggcgga aatcaaacaa gccaaattct aattggcgaa
     1981 gcacaaacat tgccgcttgg aagtttttcg ccgccaataa ttgctctaat ttcgcttttg
     2041 gtaacggctc gccggtttca taatgccctg aaataaaggc taatgcctct tcttcccagc
     2101 accagttctc taagaattgg cttggcagct ccactgcgtc ccacggcaca ccgttaatgc
     2161 cggcgacatc gcccacgtca atttcagtta gcatatggtg aatgccgtgt ccaaactcgt
     2221 ggaacaaagt tgtcacttca tcgtgggtaa acaatgccgg tttatcgcca atcggtttat
     2281 tgaagttaca ggttaaatat gccaccggtt tttgtaatgt gccgtcagcc aagcgttttt
     2341 ggttgatgca atcatccatc cacgccccac cacgtttgtt ttcacgagcg tataaatcta
     2401 agtagaacga gccacgcaag cggtcatttt tatcaaaaat attgaaaaaa cgtacatctt
     2461 tgtgccatac atccacatct ttttgttctt ccacacgcat accgaaaatg cgtttaatta
     2521 cttcaaataa gcctgataat acgcgatttt ccgggaaata cgggcgaagt tcttcatcat
     2581 tgaccgaata caatgcctgt ttttgttttt cgctgtagaa cggaatatcc cataaatcca
     2641 aatgttcaat gccgaaatgg gctttagcaa attttttcaa atcgcataat tcatttttgc
     2701 cttgagcttt cgagcgagcg gctaaatcgt tcaagaaatc taatacctgt tgtggatttt
     2761 ctgccatttt ggtggctaat gaataatcag aataagtctt aaagccgaga agttgggctt
     2821 tttcgtgacg cagttttaag atctcatcaa tcaccgcact gttatcccat ttgcctgcat
     2881 tcgggccttg atcagaagca cgagtcacat aagcacggta catttcctca cgcagtgctt
     2941 tgttttcaca atacgtcatc accggcaaat agcttgggat ttcaagagtg aagcgatagc
     3001 ctttcttacc tttgctttct gcggataatt ttgccgcttc taatgccgat tccggcaagc
     3061 ctttaagctg tgcttcatcg gtaattacaa tatcccaacc cattgttgca tcaagtacgt
     3121 tgttgctaaa ttgcgagttg agttccgaca agcgtgcaga aatttccccg tagcgttttt
     3181 gtttctctgc cggcagtgaa atgcctgata attcaaaatc ttgtaagctg ttttcaatcg
     3241 cctttttctg tgcaaccgaa taagtttcaa actcagggct gttttttaac ttcacatagc
     3301 cttgatataa cccttgatgt tgccccgccc aagtgctgta ttccgataat aacggcaagc
     3361 acgcttggta agcctcacgc aactctgggg aatttttcac cgcattcaaa tgccctaccg
     3421 gtgaccacgc acgggagaat ttatcctctg ccattgcttg tggcatatag aaattttccc
     3481 acgtcgggtt ttcaatttgt gcgacttttt caacagtttc acggcattct ttgatcaccg
     3541 cttctaccgc cggtttgata tgttcaggtt tgatttgtga gaacgcaggc aagcctgtgt
     3601 aatttaaaag agggttagac attatttttt ccttttatgg tcaaaatgaa aattcgtatc
     3661 aagataatac acaaagtgag agagaaaaga aaacaagcgg tcatatttca ataatttttt
     3721 gcaaaaaaat cacaaaatat gaccgcttgt ctttagaatt tgactgtatg cccgtaactt
     3781 tcaatgattt tttggatacg ctctaaatcc tctttaggcg gtgacattac cccttccagc
     3841 tcatatttat agcctaaggt ttcccatttg tgagccccta aacgatgata aggcagaagt
     3901 tccactttct caatgttatc cattccttga ataaactgcc ctaaacgatg ggcagaatca
     3961 tcgtcatcag tataacccgg cacgaccaca tagcgaaccc aagtcggttt atttaatttg
     4021 tgcagataac gggcgaaatc aagggtacgc ttattcgaca ccccgactaa atcttggtga
     4081 acttcatcat tgagctgttt taaatccagc aagaccaaat cggtcacttc aagcatttca
     4141 tccaccaccg ggctataatg gcgaacaaaa ccattggtat ctaagcaggt attaatgcct
     4201 tcagccttac aggcacgaaa ccaatcccgc acaaactcca tttgcaacac cgcttcccca
     4261 cccgaagctg ttacaccacc gccggttgct ttcataaaat gcttataagt cgtgacttct
     4321 ttcatcagct cttcaaccgt aatttcctta ccaccatcaa gatcccaagt atcacgatta
     4381 tggcaatatt tacaccgcat caaacagcct tgcaagaaca gaataaaacg aatccccggt
     4441 ccatctaccg taccgcagga ttcaaaagaa tggatacgtc caacaacaga catttgtcac
     4501 ctcaaccctc aaaaaatttt gagaaattgt agcaaatttt tatgttgttc gcagaaataa
     4561 atcacaccgg tatattttaa gaaaggagaa agtcatacta ttttttgcct atttcaggaa
     4621 taaactttta ttaatctatt gactccatca acatatcaac caaatatgtt taaatttttg
     4681 cattttttaa agctattttg accgcttaca agcagtcggt ttttctgaaa actttgcaaa
     4741 cctagccggc gtggtatttg gcggcgacct cttttaccag taagtcgaaa tcgctttcgt
     4801 aggtgagatc ctgcttgact cggtattttt caaattcgct gagggcgaaa gatttagcaa
     4861 tttcagcggt aactttgccg gcatcttgta agatttctcg gtcggtggcg gcaagaaagc
     4921 ggttaaggcg ggtttcccag tctgccattg tcatcggaat ttggcgttct gccatatctt
     4981 ccgccatatc caaataggca gagaccagcc gttgtagttg tgctaattca ttatccgata
     5041 aataattttt ggcaaccacc acatcaaagg ggtaaatttt gccttgcgga gcgtctttcc
     5101 aattcgttag ccccatattc ggcttttcag cgttggctcg tgccataatc agctcggcgg
     5161 cggtatgccc gtgaattgcc caatggagct tgttctgcac ggtggcaaaa aagcgtttgg
     5221 tggcggttgc ggtgcggtcg tagtccacgg cggtagcgta aatgtcggtg attttttggt
     5281 agaacttgcg ttcggataac cgaatttccc gaatacgagc aagctgttct tcaaagtatt
     5341 ttttgcccaa aatcgtgccg tcatttttca gccgttcatc gtccatcgca aagcctttaa
     5401 tcgcaaagct atgcacgatt tcggttgccc atttgcgaaa ctgcaccgct cgctcggaat
     5461 tgactttata acccaccgca ataattgccg acagattata atgcttggtg tggtagtttt
     5521 tgccgtcttg ggcagttatc caaaaaaatt ggataactga attttcagct aactcattgt
     5581 cactaaagat ttttttgaga tgatagctaa tggttcgtac atcaacatca tacaacaccg
     5641 ccatcatttt ttggctaagc catacatttt catcggcgta aacggcatca acttggcttt
     5701 cgccggtagc tgtaataaaa gtgaggtatt ccgccacaga ggaacgaatt aaatcttgct
     5761 tagacatctc tttctcccat atagaacact gtaattataa acaatattac gaaagctgtg
     5821 taaatgaata gccaattttt cgagcgagat cacaaaaaaa ccgcttgcgg cataaatgct
     5881 acaagcggtt gattattact cacaaaattg tttccaattt tgttcgctcc tacggagccg
     5941 tcggcaaagc caacgttcaa aacgcaatgc gttttgtggc taaaagtttt gcaaattgta
     6001 attacatact ttcagtgaag gtgcgggtaa ttacgtccat ttgttgctct ttggttaaag
     6061 agttgaaacg taccgcatag cctgaaacac gaatcgttaa ttgcggatat ttttcagggt
     6121 gttctaccgc atctaataat gtatcacggt ttaacacgtt cacatttaag tgctgaccgc
     6181 cttctacagt tgcttcgtgg tggaagtagc cgtccattaa gcctgcaagg ttacgttttt
     6241 gggcttcgta atctttacct aacgcatttg gtacgataga gaaagtataa gaaataccat
     6301 ctttcgcata ggcaaacggt aatttcgcca cagaagttaa tgaagctacc gcacctttct
     6361 ggtcacgacc gtgcattggg ttcgcacccg gtccgaatgg agcacctgag cgacgaccgt
     6421 ccggtgtatt accggttttc ttaccatata ccacgttaga agtgatagta agcacagact
     6481 gagtcggcgt tgcattgcgg tatgttttga gtttttgaat tttcttcata aaacgttcaa
     6541 ctaaatcaac tgcaatgtcg tccacacggt tatcgttgtt accgaattgc ggatattcac
     6601 cctcaatttc aaagtcggtt gctacgtttt tcgctacacc aaccacttcg ccagctttgt
     6661 ttttgatttc aatatcggta cggattggtt ttactttcgc atatttaatg gctgaaagtg
     6721 agtctgccgc tactgaaagc cctgcgatac cacacgccat tgtgcggaac acatcacggt
     6781 cgtgaagagc cattaatgcc gcttcgtatg catatttatc gtgcataaag tggataatgt
     6841 tcagtgctgt cacatattgg gttgcaagcc aatccataaa gctgtctaag cgggtcatta
     6901 catcatcgta atctaagtat tcacttgtaa ttgcttcagt tttaggccct acttggtcgc
     6961 ctgatttctc atcaacaccg ccattgattg cgtataacaa ggttttcgct aagtttgcac
     7021 gagcgccgaa gaactgcatc attttaccca cgatcattgg cgatacgcag cacgcaatcg
     7081 catagtcatc gttttggaaa tccggacgca ttaagtcatc gttttcatat tgaactgatg
     7141 aagtatcaat agacactttc gcacagtagc gtttaaatgc ttccggtaat tgttcagacc
     7201 aaagaatagt taagtttggc tccggtgacg gccccattgt gtaaagggtg tgtaaaatac
     7261 ggaagctgtt tttggttact aaggtacgac cgtctaaacc cataccggct aaggtttcgg
     7321 ttgcccacat tgggtcgcct gagaataatt gatcgtattc cggagtacgc aagaaacgca
     7381 ccatacgcaa tttcattact aaatggtcga ttaactcttg ggcttcttgc tcggtaattt
     7441 tgccggcttt taagtcacgt tcgatataaa tatctaagaa tgaagacaca cgaccgaaag
     7501 acatcgccgc accgttttgc gatttaaccg ctgcaaggta agcgaaatat gtccattgca
     7561 ccgcttcttg tgcattggtt gcaggacctg aaatatcaaa accgtagctt gcagccatct
     7621 ctttcatttt gcctaaagca cggtgttgct cggcaatttc ttcacgcagt tgaatcgtgg
     7681 cttgaatatc ttcgccggat tctaaacgag cttgtaatga ggcaaactgg cgttgtttgt
     7741 ctttcattaa gaagtctgca ccataaagtg ccatacggcg gtagtcaccg ataatacgac
     7801 cacgaccata agcatccggc aaaccggtaa ttacacctga tttacggcaa cgtaaaatat
     7861 ccggagtgta aacatcgaat accccttggt tatgggtttt acggtattcg gtaaagattt
     7921 gttctacttc aggtttaagc tcacggcgat aaactttgca cgaacctttc accatattga
     7981 taccaccaaa cggcataatc gcacgtttta atggagcatc tgtttgcaga ccaacgattt
     8041 tttctaacga tttatcaata taacccggtg cgtgtgaggt aatggttgat ggcgtgtcgc
     8101 agtcaatatc atacggttcg tgtgttttgt tttctacttt gatcatttcc atcacatctg
     8161 cccacaattt gctggttgca ggtgttactt cagctaggaa agactcatca ccctcataag
     8221 gtgtgtagtt tttttggata aaatcacgta catttacttc agtttgccac tcgcctgcgg
     8281 caaaaccttc ccacgctttt tgttgagcgt ctgttagttg agtcatttta agtcctctgt
     8341 gttagtaagt taaaacggga aagcggtgat tttttgcaaa aaatttgcaa aatttgaccg
     8401 cttgaatctg aaatcttaat gctttctaat gtataagaac cactgcatta aaccaatgaa
     8461 aaacacgcca ccgacaatat tacccagcgt tgccggaatt aagttcttaa acacaaaatg
     8521 agatacatct aaatcggcaa attgcgttgg gtcaacatta attgctgtcc aaaattcagg
     8581 gctggcaaag tgcgaaatcg caatccccat cggaatcata aacatatttg ccacacagtg
     8641 ttcaaagccg gaagccacaa acattgcaat cggtaacatc ataataaagg ctttatcggt
     8701 taggctttta cctgcataag acatccacac cgcaatgcac accataatgt tacagaaaat
     8761 gcctaaacag aaagcctcaa accattggtg gtgaattttg tgctgtgcgg tatttaaaat
     8821 aaccaaaccc cattgccctt tggctgccat aatctgacca ctaaaccaaa tcaacaatac
     8881 aatggaaatc gccccaacaa agttaccgaa atacaccact acccagtttt taaacatctg
     8941 taaccagtta attctttggc tggctctggc tacaatcgta agtgttgatg aggtaaaaag
     9001 ttctgcccca aacaccacac acataatcac gccgagtgaa aataccaaac caccaatcag
     9061 tttagccata ccccaaggca tttggctcgc acccacttgg gttgtggtat aaaatacaaa
     9121 tgctattgca ataaaaccgc ccgcagtaat ggcagagaga aaggaatata attgattttt
     9181 tgttgcttta taaaccgcac catcttctgc gatttttgcc atttcagcag gagaaaacat
     9241 cagacaacct taacaatgag aattgaaaaa ccgatatatt atgtagaaat tatagacctc
     9301 ttgagataat cttcaaataa aaatattaca caatcagagg tatttcgatg tctttttttg
     9361 atatagaaca aaatccataa aaaacacaac attcaagaaa atttataact acattttaat
     9421 aacaaaccaa ctacaatttt atgacagaat atctcacttc ctttttcagt ttattctttt
     9481 ccgaacaaaa tcaactcttt actatgtttc tgagtggatt tctaagcgct accctcttac
     9541 ccggaaattc tgaaattatc ttcactacct tagcaagcca acatctcatc acttatggct
     9601 ctttatcctc attttcactg ttcaatttac tcttggttgc aaccttaggt aatagtttag
     9661 gtagcataac cacttatgcg atagggctat tactccctcg cccacagaat actaccaatc
     9721 gttattcaca atgggcatta gccaaaagtg aaaaatatgg cgtgttagtc ttgcttttta
     9781 gttggttacc gattgtaggc gatattttct gtggcattgc cggctggcta cggtttagcc
     9841 tttggcaaag cattctcttt attgtgattg ggaaattgtt acgttatctg tttttgctgg
     9901 caactctcta ttcggtgtta ccgcatattt tatagaaaac aaaagcggct gttttgcaaa
     9961 aaattttaca aaaacaaccg cttgtgcttt ataagtttct taacgcttca atacgttttt
    10021 ctaacggagg gtggctcata aacaactctt taccacggct accgttaatc ataaaggcag
    10081 caagagagcc ttccatttct tgcggttcgt gaacacgttg caagcgttgt aatgccgcaa
    10141 tcattttctc tttaccgact aattgagcag agcctgcatc tgcacgaaat tcacgttggc
    10201 gagagaacca cattgcaatc attgttgcta acacaccaaa caccatctgt aacacaatat
    10261 ctactaccca gaacaccagc gtattggtgc ttgaattgcc attttcgtca cgtgaacttg
    10321 ccgccattgt cgaaataata cgagctaaga aaatgacgaa agtatttaac acgccttgta
    10381 ataaagtcat tgtaaccata tcgccattcg caatgtgcgc cacttcgtgt gctactaccg
    10441 cttcagcttc gtctttagtc attgaattca gcaagccggt gctgactgca actaaagaat
    10501 tacttttcgt tgctcctgta gcgaatgcat ttacatcagc cgaatggtaa atcgcaatgt
    10561 ccggcattgg gatattcgct tgctgagcct gacgttgtac agtgttgaat aaccattgct
    10621 ccgcttcgtt acgaggctgt gtaatcacct ctgccccaac cgaacgcaat gccattgatt
    10681 tcgacataaa tagcgaaatt aaagacccac tgaaaccgaa gactaacgaa agcaccaaaa
    10741 taccggcagt gctttgccct tgaatgccgg tcactttaaa aataaggttt aatacgatcc
    10801 ctaaaacaaa ggtaatcgct aagttagtta ataaaaatag aatgacacgt tttgccataa
    10861 tgttttcaat ctccaaaaat atagatgcaa tgattgcacg aataagataa tgactttagc
    10921 cttaatttca agtcattatt tacaattatt cccacaaatt taactgttta ttcacttttt
    10981 tctcgggaaa gtgaacactt agaccgatta atcgcacctt tcgttccttt cttcgggtaa
    11041 aaatttgctg tagcaactcg ccaaatcgct catacgcaaa gccatcggtg gttcgttcaa
    11101 gcgtggtttg gctaaaatca tcaaacttga gtttaatgcc taacttttta aactcagtta
    11161 atggcgtatc gccccagttt cgttgcaacc gaaaaaccag tttgtcgaat aaatctgcca
    11221 caatcttttc cgcttgctca agggttgaaa tatcgtgcaa cagcgtattt tccaccgcca
    11281 gcgattttct ttcacgataa ggctctaccg ctcgttcatc aataccgtgg ctaaaatccc
    11341 aaagccgttg cccgaatttg ccaaacgctc ggtaaataac cgctttttct gcttgctgaa
    11401 tatcaccaca ggtttcaaag ccaagctgtt ttaatttttc attggttact ttccccacac
    11461 ccggaatttt tgcaagcggt agatttttca caaaattttg cacatcatca ggcgtaatca
    11521 cacattgtcc gttaggtttg ttttgatcgg aggtaatttt tgctaaaaat ttaagcggag
    11581 caactcctgc cgaggcagtt aaatgcaatt cattgaaaat atcattgcgg attgcttgag
    11641 caatccaagt ggcggagcca aaatagcgtt cgcattcggt tacatctaaa taggcttcat
    11701 caagcgacaa ggcttcaatc acatccgtat agcgatgaaa aatttcgtga atttgagcgg
    11761 aaaccgattt atacaacgcc atattcaccg gcaataaaat taaattcggg catttcttca
    11821 acgcagttgc cgttggcatt gcactgtgta agccgaattt tcgagcctcg taattacagg
    11881 ttgtcagcac acctcgttga ttagcattgc cgcccaccgc caccggctta ccgaccaacg
    11941 ccgggttttc ccgcatttcg accgctgcat aaaaacaatc catatcaata tggataattt
    12001 ttcgcttatg tttagcttga atcgacattt gcaatatttt ccccaaaaac gaccgcttgc
    12061 aacacaaata actgtatata aaaacagttt tattttatgc ccttattgtt agaatttcaa
    12121 gtttaaaaat agcgataaaa aggataaaaa atgctctacg gtttagccat tgtgctgatt
    12181 ccactttttc tcggttactt aataaccctc aaaaaccacc gccatatggc aatagtaaac
    12241 agagtggtca gcctctgcct ttacttgatt ttattagtga tggggatttc tctcggacaa
    12301 attgataaca ttctcaccga actgccacag attggcggta cggcgtttag tttagtggtg
    12361 attttactcc tttcaaacat tatcggcttg gtgatttacg acaaattaca accgatgaaa
    12421 agagacaaag tcaatcaaga tcaacttccc tcccgcctgc atttgttgat tgattccttt
    12481 aaattaatcg gcattacctt attaggcggg atcatcggct atttcaccaa aggcgtggtg
    12541 gatttccctc tgcacgccag cacctatgtg ttagaagtga tgattttcgg ggtggggatt
    12601 caactgcgaa acagcggtat tcccctgcgt gaagtgttct ttaacaagcg ggggatttat
    12661 acctcgattg taatggtggc aagttccctg attggtggcg tgatttcggc attcacctta
    12721 gatttatccc ttgctaaagg cttaaccttt gcctctgcat tcggttggta ttccctttca
    12781 agcgtattgg taaacgatgc ttggggcccg atttacggca gtattgcctt ttttaacgac
    12841 atctcgcgtg aaattttatg cctgttttta gtaccactgt ttatgcgtca atttccgtcc
    12901 accgcagtcg gcattggcgg agcgacttca ttagattgta tgctccctat tattcaaaaa
    12961 tcgggcggta ttcaaattgt gccgctggca atcagcttcg gttttattgt taatttagcc
    13021 gctccattgt tattagccgt ttttattggg ctggaaggat aaatcaccgc ttgtttgctt
    13081 ttatttatcc atcttcgctg aattttccgc tataatcctt aagttttccc cgaaacttgg
    13141 gatatttatt ttcaaaagga taattttatg atgcgttctc actactgcgg tgcattaaac
    13201 cgctcacacc ttggacaaac agttacctta agcggttggg ttcatcgtgt tcgtaacctt
    13261 ggtcgtttta tttttatgca aattcgtgat cgtgaaggca ttgtgcaggt gtttttcgat
    13321 gagaaagatg aagcactttt caagcaagca tcggccttac gttctgaggc gtgcgtgcaa
    13381 attcaaggtg aagtgattgc ccgtgatgaa tcgcaaatca ataaagagat ggcaaccggt
    13441 gaaattgaag tgttagtaaa aaatttagtg gtgtataaca acgctgaagt attgccgtta
    13501 gacttcaatc aaaacaatac cgaagaacag cgtttaaaat accgctattt ggatttacgc
    13561 cgccctgaaa tggcagaaaa gctcaaaacc cgtgctaaaa tcaccagttt tgtccgccgt
    13621 tttatggacg ataacggttt cttagacatt gaaaccccga tgctgaccaa agctactcct
    13681 gaaggtgcga gagattacct cgtgccaagc cgtgtgcata aaggcaaatt ctatgctctg
    13741 ccacaatcac ctcagctatt taaacagttg ctaatgatgt cgggcttcga ccgttattac
    13801 caaatcgtga aatgtttccg tgatgaagat ttgcgtgccg accgccagcc ggaatttacc
    13861 caaatagacg tggaaaccag ctttatgact gccgaagaag tgcgtgcatt aatggaagcg
    13921 atgatccacg gcttatggaa agagacctta ggcgttgatc tcggtaaatt cccaatgatg
    13981 acgtggaatg aagcaatgac ccgtttcggt tcagacaaac cggatttgcg taacccactg
    14041 gaaatggttg atgtggcaga tattttaaaa tcggttgatt tcaaagtatt tagcggtccg
    14101 gcgaatgatc cgaaagggcg tgtagtggca cttcgtgtgc caaacggtgc agctctaacc
    14161 cgtaaaaata ttgatgacta cacccaattt gtcggcattt atggggcaaa aggcttggct
    14221 tggttaaaag tgaatgatat gaacacaggt ttagaaggca ttcaaagccc ggttgcaaaa
    14281 ttcttaaatg aagaagtgct aaaagcctta gttaaacgct taaacttgca aaaagacgac
    14341 attgtattct tcggtgcgga taactggcac gttgcgacta acgccatcgg tgcgttacgc
    14401 ttaaaagtgg ggaacgattt aggcttaacc gataccacag cgtggaaacc gttatgggtg
    14461 attgatttcc caatgtttga acgtgatgat gaaggcaact tatcggcaat gcaccacccg
    14521 ttcacttcac cgaaagaatt atcgccggaa gaattagtgc aagatccgga aaatgcggtg
    14581 gcaaacgctt acgatatggt aattaacggc tacgaagtgg gcggtggctc ggtacgtatt
    14641 ttcagccagc agatgcaaca aaccgtgttc agcattttag gcattaacga agaagagcaa
    14701 aaagagaaat tcggtttctt attagatgcg ttaaaattcg gtactccgcc acacgcaggc
    14761 ttagccttcg gtttagaccg cttaacgatg ttgattacag gaactgataa cattcgtgat
    14821 gtcatcgctt tcccgaaaac caccgctgca gcgtgtttaa tgacagaagc accaagctat
    14881 gcgaatccga aagcgttgga agagttagca atccgtacaa ttccacaaga gtaatcggta
    14941 ggggcgaaac atttttcgcc ctttttattt aacaagcggt cgaaaaagtg aaaaactttg
    15001 caaaagtaat aatcgcaaaa ttattctcta aataacataa taaagactct tttctcttct
    15061 ctatgttact actaaatatc ccctattatt ttaattatta ccttatttgg gtattgagca
    15121 gattta
//
LOCUS       DANLXF010000047        14683 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.047, whole genome shotgun sequence.
ACCESSION   DANLXF010000047 DANLXF010000000
VERSION     DANLXF010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14683
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.047"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            148..1071
                     /gene="oppB"
                     /locus_tag="PXD66_000865"
     CDS             148..1071
                     /gene="oppB"
                     /locus_tag="PXD66_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HDL6319188.1"
                     /translation="MLKFIFKRLLEAIPTLLILITFSFFLMRLAPGSPFTSEKAYPPK
                     VMANIEAKYHLNEPLYKQYFLYMENLSKGDFGPSFKYKDQSVNNLIASAFPVSIKLGI
                     TAFIFAVLIGLTAGTIAALKQNSKWDYLVMGFSMTGIIMPSFVFAPLLVLMFAIYLKW
                     LPAGGWNGGQLYYMVLPVLSLTISYVAGIARITRGSMIEVLHSNFIRTAKAKGLPMRK
                     IIFKHALRPALLPVITYLGPAFVGIITGSMVIESVFGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGTLTILFNAIVDILYAVIDPKIRYS"
     gene            1086..1985
                     /gene="oppC"
                     /locus_tag="PXD66_000866"
     CDS             1086..1985
                     /gene="oppC"
                     /locus_tag="PXD66_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HDL6319189.1"
                     /translation="MQNSSKNQPLVEVTSTIQGRSLWQDARRRFFRNKAAVASLIILC
                     CIILFITFAPMLMPFSYEDTDWNMMGIAPDFESQHYFGTDSSGRDLLVRVAIGGRISL
                     MVGIAGALIAVLIGTIYGAISGYFGGKVDMVMMRFLEILSSFPFMFFVILLVTFFGQN
                     IFLIFIAIGMVAWLNLARIVRGQTLSLKNKEFVEAAIVCGVSRRQIIWRHIVPNVLGL
                     VVVYASLEVPSLILFESFLSFLGLGTQEPMSSWGALLSDGAAQMETSPWLLAFPAFFL
                     CLTLFCFNFIGDGLRDALDPKDK"
     gene            1995..3590
                     /locus_tag="PXD66_000867"
     CDS             1995..3590
                     /locus_tag="PXD66_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL6319190.1"
                     /translation="MELLAVNNLDVYLKTDEELVHAVRNVSFSIEKGQTLAIVGESGS
                     GKSVTSMAIMQLLPKNIVSFGERSSIIFEEQNLLTLSEKAMQTVRGDRIGMIFQEPMT
                     SLNPYMPIGKQVAETVQTHNPQISNAEAEKMVLETLQKVKIPDAEKKMICYPHEFSGG
                     QLQRIMIAMAIINKPDLLIADEPTTALDVTTQAEILDLMHDLQRDMGMAIILISHDLR
                     LVHKYSDLVCVMQNGEIIERGETETVFTNPQHPYTVELLTPIPTNLKGELPPNAPTLL
                     QGEHIKVDYILKRSLFGKPKKVFNALKDISLHLKTGETLGIVGESGSGKSTLGRAIMQ
                     ILDYRGNITFAGKTLANLNKAERQALKKDMQMVFQDPFNSLSPRLTVGEIIAEGLTVH
                     CPQMSKTERREKVMKMLEEVNLNPAMINRYPHEFSGGQRQRIAIARAIILEPKFVLLD
                     EPTSALDRSTQITVIELLNRLQKKYGLSYIFISHDLAVIKALSDRVIVMSQGEIVESG
                     DVTQIFENPQQEYTKRLILASNL"
     gene            3770..4165
                     /locus_tag="PXD66_000868"
     CDS             3770..4165
                     /locus_tag="PXD66_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL6319191.1"
                     /translation="MKKLMIFATTAMIVSNLAHAAGEQSDAREAHESTVKTSTVKYSC
                     QNGKKLSVKYGFNKQGIPTYAEAKLSGKKRFMPINLYTTDATGTNFGDENNFSLYGDP
                     MEFTNHRKASVNIQSPASEILYKGCTPQK"
     gene            complement(4375..6534)
                     /locus_tag="PXD66_000869"
     CDS             complement(4375..6534)
                     /locus_tag="PXD66_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HDL6319192.1"
                     /translation="MQIKQTVLAIAITPFISQLSLAQETKISSIELDTIEVNEQQENL
                     LKQQAVMSNEDLVRYSTDMGVPQTGRFGPQGFNIRGVEGNQVAVTVDGISQAASNNYA
                     TFSRYSYYNKSRPEIDTELMSDVTIEKGSSTKVGNGALGGSVNYKTKTVSDILMKGRQ
                     FGGMLKYTYNGQNKENIETAGIAADGQYADALVMYSHRQGHETQTTSKGPDIMGSGRG
                     LPNPYKNNQYSYLAKLGLKWNDENRLELKTSKQRSKNAGQELSYSTVTLRDFLDVQEI
                     ETYGVTYLYTPKNSILENIKVAVTKQDTEISGINDQITIARGTKTDNTKRAFNTKNVE
                     GSLGVTFKPVDLGNTEHKLSTELGYGVSDFSFDMQTYTAGITPTDQNMQSPAKTKSFH
                     ILMGDSILLTQNLSAHLNARYEQSKLHSDDNSLSDKTFNNWAGDIGLNYQLSPVWQIG
                     YKLSRGFRTPTASEMFFNREVQGRGASQTFLANTNLKPETSLGNEISLVGNGSLGSLS
                     VAAYHTHYRNLIDLATIGNNIYQSQNVNRAKIKGVDINGTLDLNTAWNAIPQGFELNG
                     GFGYARGKRNDGTDLLTVQPLKALFGIGYRAVNNDWAIHLNGTYTQGKKAKYAQQYAI
                     LANTGYLSYDKSLTTYPYLSNSATVFDLVAHKKFGERAIVKVGVYNLFNKKYWTWDSL
                     RTIGITSPGVANSITGEGLNRYLAPERYVMASVEFKF"
     gene            6641..7507
                     /locus_tag="PXD66_000870"
     CDS             6641..7507
                     /locus_tag="PXD66_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL6319193.1"
                     /translation="MEYKMRTLLFSFIALSMCLNTTKAAEKIVSTAGYASEIVAALGK
                     ADKLVGVDTTSVKPQTIMEKKPKIGYRRQLSAEGILSLTPDLIILAPDAGPNAVIEQI
                     KASGIPLLHLGEKPSLAGIREDIQQIAKAIGAESEAKQLTETILADEKKLADIRSQHS
                     TDKEVLVLIDTASQGIFGLGKKSVGDHFLTLLNLNNSFNAEGNKPLANEALAASNADV
                     ILLASRNQAKESATISKLAESSPQYAQLTNTQAGKKGCVFAINILDALGFGPYTAQYA
                     HQILTTIQPCLK"
     gene            7518..8504
                     /locus_tag="PXD66_000871"
     CDS             7518..8504
                     /locus_tag="PXD66_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL6319194.1"
                     /translation="MKLHLTLIFTLIISLVICTHLGAMSLPLSAWHCNNAECESAAYI
                     LWQIRLPRLLGALFIGAALAISGATMQGLFRNPLVDPGIIGVSSGAALAAALYIVVIQ
                     PLLPESISYYALPIAAFLGGWLVTFMLYLLSQRNGQLHIAIMLLIGIALAAFSGALTG
                     ILIYVADDNQLRSLTFWSMGSLAGMNWQMVAMMAAVVFICSPLIWREHRALNALTLGE
                     EDAQYIGFDIAKVKRRLVFYVALLTGTSVACVGAIGFVGLVIPHLMRLLGGSNHRHLL
                     LNSLVCGAILLMIADTLARTLAAPAEIPIGIFTALFGAPFLAVMVWKSGKHI"
     gene            8504..9310
                     /locus_tag="PXD66_000872"
     CDS             8504..9310
                     /locus_tag="PXD66_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL6319195.1"
                     /translation="MSNLLTCQNLSIVHQQKKLLEIQEIAFEQGKFTAIIGPNGAGKT
                     TLLKALSGNNAQTNSAVFYQHQRLTDLNGYALAQKRAMLSQQSQIAFPLKVEELVQLG
                     REPYRQSEFAKFNEKITAWAMETMSLTALKERSSIQLSGGEQHRAHIARVLAQLVPEP
                     NADLSGSWLLLDEPTNHLDIHHQYQLFSLLQKLKQQGLTIIAILHDPALAINQADHIL
                     MLKNGEKFAEMSAKAIAESCLLDELYEMKMGCRYCPHSGQYYLCPALAES"
     gene            complement(9331..9969)
                     /gene="purN"
                     /locus_tag="PXD66_000873"
     CDS             complement(9331..9969)
                     /gene="purN"
                     /locus_tag="PXD66_000873"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDL6319196.1"
                     /translation="MKKFVVLISGNGSNLQAMIDAQKSADTSGQICGVICNKADAYGL
                     IRAKQAGIPTFVFSRKDYQSNVEMDLAIAEQIEQLGAELIVLAGYMKILTPEFTQHFA
                     GKILNIHPSLLPKYPGLNTYQRAIEAGESEHGTTIHFVNEEVDAGAVVLQAKVPIYPE
                     DEIEDVMARVVEQEHRYYPLVIEWFCSGRLVSQHGKAYLDGKLLPACGYAED"
     gene            complement(10076..12233)
                     /locus_tag="PXD66_000874"
                     /pseudo
     CDS             complement(10076..12233)
                     /locus_tag="PXD66_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484758.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(12287..13123)
                     /locus_tag="PXD66_000875"
     CDS             complement(12287..13123)
                     /locus_tag="PXD66_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HDL6319197.1"
                     /translation="MLPRISSFLASLFMAFFLLGCSVSTQSLNEQNVDSIGQIIDLNT
                     NQTISFTELLSRLSSQDYVLVGEEHTQLIHHQIELYLFNQLTKKAKLHLIALEMLNVD
                     QQPAIDRIQFNRPVNLSATDLREAIQWQKWDWKMYQDLVVESLGSNSRVIATNLTDKE
                     VEILLNGAEPLKGSVSTSSEVKQKIAQLLLSMNHGMPYPMENMVAVQQFRDRRMAEKL
                     VKNALSTSLLIAGNHHVRKDLGVPLHIAEYDRTKKVAVLMLKTEREEITSSQADYLWV
                     TQ"
     gene            complement(13281..14543)
                     /locus_tag="PXD66_000876"
     CDS             complement(13281..14543)
                     /locus_tag="PXD66_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDL6319198.1"
                     /translation="MLKRDMNIADYDAVLWQAIQDEDRRQEEHIELIASENYASPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKALFGADYANVQPHSGSQAN
                     AAVYMALLNPGDTILGMSLAHGGHLTHGASVSFSGKIYKAEQYGITDEGLIDYEALRK
                     QAQEVKPKMIVAGFSAYSQVMDWAKMREIADEVGAYLFVDMAHVAGLIAAGVYPNPLP
                     HAHVVTTTTHKTLAGPRGGLILSASGDEEMYKKLQSAVFPAGQGGPLMHVIAAKAVCF
                     KEALEPEFKAYQQQVVKNAKAMVEVFKQRGFDVVSNGTENHLFLVSFVKQGLTGKAAD
                     AALGAANITVNKNSVPNDPQKPFVTSGIRVGTPSVTRRGFKEAEVTALAGWMCDVLDS
                     IGKDNHEQVIAETKAKVLDICKRLPVYA"
BASE COUNT         4300 a         3052 c         2805 g         4526 t
ORIGIN      
        1 cgattttttt acaaaaataa gctaaaaaaa caaccgctta agaataatga attcttataa
       61 aaatagccat aactaatcaa tatctttaca aagccgttaa tttacgctat taattgccta
      121 ccaatataac taaaaagagt ggtgcttatg ctgaagttta tttttaaacg tcttcttgag
      181 gcgattccaa ccctactaat tctgattacc ttttcctttt tcctgatgcg gcttgccccg
      241 ggtagcccat ttaccagtga gaaagcctat ccgccgaaag taatggcaaa tatcgaggca
      301 aaatatcacc tgaatgaacc gctgtataag cagtattttc tttatatgga aaatctgtca
      361 aaaggcgatt tcggtccatc ttttaaatat aaagatcaat cggtaaataa tctgattgct
      421 tctgcgtttc ctgtttcgat aaaactgggc attactgcct ttatttttgc ggtgcttatc
      481 ggtttaaccg caggaactat cgctgcactt aagcaaaata gtaaatggga ttatttagtg
      541 atgggctttt caatgacggg tattattatg ccgagctttg tctttgctcc gctgttggta
      601 ctcatgtttg cgatttatct caaatggttg cctgccggtg gttggaacgg tggtcagctc
      661 tactatatgg ttttacctgt tctctcttta accattagct atgttgccgg cattgcccgt
      721 attactcgtg gttcaatgat tgaggtgttg cactctaatt ttattcgcac cgctaaggca
      781 aaaggcttgc caatgcgtaa aattattttc aaacacgctt tgcgtccggc attgttgcct
      841 gtgatcactt atttaggccc ggcttttgtt ggcattatta ccggctcgat ggtaattgaa
      901 agcgtgtttg gcttaccggg aatcgggcag ttgttcgtaa acggagcatt aaatcgtgac
      961 tattcattag tattaagcct aaccattttg gtaggtacgc tcaccatttt atttaatgcg
     1021 attgttgata ttttatatgc ggtcatcgac ccgaaaattc gttattcata ggagtaaagc
     1081 ggaaaatgca aaattcttca aaaaatcaac cgcttgttga ggttacctca accattcaag
     1141 gacgaagttt atggcaagat gcacgccgcc gttttttccg aaataaggcg gctgtggcaa
     1201 gtttgattat tctttgttgt attattcttt ttattacctt tgccccaatg ctaatgccgt
     1261 ttagctatga agataccgat tggaatatga tggggattgc tccggatttt gaatctcaac
     1321 actatttcgg tactgattct tcagggcgtg atttgctggt tcgagttgca atcggtggac
     1381 gcatttcgtt aatggtaggg attgccggtg cattaattgc ggtgcttatc ggtacaatct
     1441 atggagcaat ttccggttat ttcggcggta aagtcgatat ggtgatgatg cgttttctgg
     1501 aaattttaag ctcattccca tttatgttct tcgtaatttt gctggttacg ttcttcgggc
     1561 agaatatttt cctgattttc attgcaatcg gtatggtagc gtggctgaac cttgcccgta
     1621 ttgtacgtgg tcaaactctt tcgcttaaaa ataaagagtt tgtggaagca gcgattgtgt
     1681 gtggcgtttc ccgccgtcaa attatttggc gacacattgt gcctaacgtt ttaggcttag
     1741 tggtggttta tgcctccctt gaagtaccat ctctgatttt gtttgaatcg ttcttaagtt
     1801 tcttagggct tggtacacaa gagccgatga gcagttgggg ggcattatta agcgacggag
     1861 cggcacaaat ggaaacctcg ccttggctac tggctttccc tgcctttttc ttatgtttaa
     1921 cactgttttg ttttaatttt attggtgatg gcttgcgtga tgccctcgat ccaaaagata
     1981 aatagaggtt tagaatggaa ttattagcag taaataatct cgatgtttat ttaaaaacgg
     2041 acgaagaatt agttcacgca gtgcgtaatg tctcgttctc gattgaaaaa gggcaaaccc
     2101 ttgcgattgt gggggaatcg ggttcgggta aatccgttac ttcaatggca attatgcagt
     2161 tattgcctaa aaatatcgtc tcattcggtg aacgctcaag catcattttt gaagaacaaa
     2221 acttgcttac tctatctgaa aaagcaatgc aaacagttcg aggtgatcgc atcggtatga
     2281 ttttccaaga gccaatgacc tcactcaatc cttatatgcc gattggcaag caggttgctg
     2341 agacggttca aacccataat ccgcaaatca gcaatgccga agcggaaaaa atggtgttgg
     2401 aaactttgca aaaagtaaaa attcccgatg cggaaaagaa aatgatttgc tatccgcacg
     2461 agttttccgg cggtcagcta caacgaatta tgattgcaat ggcaattatc aacaaacccg
     2521 atttattgat tgccgatgag ccgaccaccg ccctggatgt cacaacccaa gcggaaattt
     2581 tggatttaat gcacgactta cagcgtgata tgggaatggc gattatctta atttcccacg
     2641 atttacgctt ggtgcataaa tacagtgact tagtctgcgt aatgcaaaac ggcgaaatta
     2701 ttgagcgagg cgaaacggaa actgtgttca ccaatccgca acatccttat acggtggagc
     2761 tacttacccc gattccaact aatttaaaag gggaattacc gccaaatgca ccaaccttgc
     2821 ttcagggtga gcatattaag gtagattata ttttaaaacg ctcgctattc ggtaagccga
     2881 aaaaggtgtt taatgcgtta aaagatattt cattgcactt gaaaaccggc gaaacgctcg
     2941 gcattgtagg cgaatccggc tccgggaaat ctacccttgg gcgtgccatt atgcagattt
     3001 tagattatcg cggcaatatt acatttgcag gtaagacact ggctaatctg aataaagccg
     3061 aacgtcaagc cctgaaaaaa gatatgcaaa tggtgttcca agacccgttc aactcacttt
     3121 ctcctcgctt aacagttggg gaaattattg ctgagggctt aaccgtacat tgccctcaaa
     3181 tgagcaaaac cgaacggcgg gaaaaagtaa tgaaaatgtt ggaagaggtt aatctcaacc
     3241 ctgcaatgat taaccgctat ccgcacgaat tttcgggcgg acaacgccag cggattgcga
     3301 ttgcaagggc aatcatttta gagccgaaat ttgtattgct tgatgagcca acctcagcac
     3361 ttgaccgctc tactcagatc actgtaattg aattgcttaa ccgcttacag aaaaaatacg
     3421 gcttaagtta tattttcatc agccacgatt tggcggtcat caaggcactt agcgaccgag
     3481 tgattgtaat gagccaaggt gagattgtag aaagcggtga tgtcactcaa atttttgaaa
     3541 atccacaaca agaatacacc aagcggttga ttttagcctc taatttgtaa ccccttccac
     3601 aaggtcttat tctaccaaaa aacaagacct tgtcatttta tgctagcaaa acattgtaat
     3661 tttgatattt tatttcaatt ttttgaaaaa atatggaact ttatataaaa ctcactttct
     3721 aaacaatacg ttatctgaac taagataacg cttatttaaa ggaaaatata tgaaaaaatt
     3781 gatgatattc gcaacaactg ccatgattgt aagcaattta gcccatgcag caggtgaaca
     3841 aagtgatgca agagaagccc acgaaagcac cgtaaaaacc tcaactgtta agtatagctg
     3901 ccaaaacggc aaaaaattaa gcgtgaaata cggcttcaat aaacaaggca ttccaaccta
     3961 tgctgaggca aaattaagcg gtaaaaaacg ttttatgccg atcaacctat acacgacaga
     4021 tgcaaccggc actaactttg gtgacgaaaa caatttcagc ctttacggtg atccaatgga
     4081 atttaccaac caccgtaaag catcagtaaa tatccaaagc cccgcaagtg aaattttata
     4141 taaaggttgc acacctcaaa aatagataaa aagtaaaatg agtccgctct gataagtggc
     4201 tttttaatag ctgtttattg cagataggaa agcattttgt ttttatctac tatccagaaa
     4261 caagcggtta aatttgttta attttttaca aaaaatgggc caaaattaac cgcttgttta
     4321 ttactataaa gaaataagta cgtaaaaatt aactcttaca tttaatgctt atctctaaaa
     4381 tttaaactcc actgatgcca ttacatagcg ctctggagca aggtaacgat ttaagccttc
     4441 gccagttata ctgttggcaa ctccaggact agttatacca atagttctta aactatccca
     4501 agtccaatat tttttattaa agagattata aaccccaact ttaacaatcg ctctttctcc
     4561 aaactttttg tgtgcaacta agtcaaatac ggtggctgaa ttacttaagt atgggtaagt
     4621 ggttaatgat ttatcgtaac ttaaataccc agtattggct aaaatagcat actgttgtgc
     4681 atattttgct tttttgcctt gtgtgtatgt gccgtttagg tgtatcgccc agtcattgtt
     4741 cacagctcga taacctatgc cgaacaatgc ttttaacggt tgaaccgtga gtaagtcggt
     4801 accatcatta cgtttgccac gagcataacc aaatccacca ttaagctcaa agccttgtgg
     4861 aattgcattc cacgctgtgt ttaagtctaa agttccgtta atatcaacac ctttgatttt
     4921 agctcggtta acattttgcg attggtaaat attgttacca atagtggcaa gatcaatcag
     4981 atttctatag tgggtatggt aagcggctac gctcaagcta cctaatgaac cattacctac
     5041 cagtgaaatt tcattaccta agcttgtttc cggttttaag ttagtatttg ctaagaaagt
     5101 ttggcttgct ccacgccctt gaacttcacg gttaaagaac atttcacttg cggtaggtgt
     5161 tctaaaacca cggctgagtt tatagccaat ttgccataca ggagagagct gataatttaa
     5221 accaatatct cccgcccaat tgttaaaggt tttatctgac aaactattat catcagaatg
     5281 taatttcgac tgctcatatc gagcatttaa atgagcagat agattttgtg tcagtaaaat
     5341 agagtctccc atcaaaatat ggaaagactt ggtttttgcc ggagactgca tattttgatc
     5401 ggttggcgta atacctgctg tataggtttg catatcaaaa ctgaaatcag atacaccata
     5461 gcctaattcc gttgacaatt tatgttcggt attacctaga tcaacaggct tgaatgtaac
     5521 acctaagcta ccttcaacat ttttagtatt aaaagcccgt ttggtattat ctgtttttgt
     5581 accacgggca atagtgattt gatcgttaat acctgaaatt tcagtatcct gtttggttac
     5641 cgcaacttta atattttcaa gaatactgtt ttttggagtg tataaataag taacgccata
     5701 agtttcaatt tcctgcacat ccaaaaaatc acgtaatgtg acggtagaat aagaaagctc
     5761 ttgaccggcg tttttactgc gttgcttaga agtttttaat tcaaggcgat tttcatcatt
     5821 ccattttagc cctaattttg ccaagtagct gtattgatta tttttgtaag gatttggtaa
     5881 accacgacca cttcccataa tatccggacc tttagacgtt gtttgtgttt cgtgtccttg
     5941 acggtggcta tacataacca aagcatccgc atattgacca tccgcagcaa tacctgccgt
     6001 ctcaatattc tctttatttt gaccgttata ggtatatttc aacatcccac caaattggcg
     6061 acctttcatt aaaatatcac ttacggtttt agttttatag ttcacagagc cgcctaatgc
     6121 cccattgcct acttttgtgc ttgagccttt ttcaatggtt acatcgctca ttaattcagt
     6181 atcgatttca gggcgagatt tattgtagta actatagcga ctaaaggtgg catagttatt
     6241 tgatgctgct tgcgatatgc catcaactgt tactgccact tgattacctt caacaccacg
     6301 gatattaaac ccttgtgggc caaagcggcc tgtttgcgga acgcccatat cagtactata
     6361 acgtactaaa tcttcattgc tcatcaccgc ttgctgttta agtaggttct cctgctgctc
     6421 atttacttca atagtatcta gttcaatgct actaatcttt gtttcttgag ctaaggagag
     6481 ttgagaaata aagggagtaa tcgctattgc taatacagtt tgctttattt gcatatttta
     6541 gatcctctag ttagtttaat ccgtttaatg ttaacactat atcattttaa ttgaaaattg
     6601 aaataataat tattattatt taagtttaga taaaaatcaa atggagtata aaatgagaac
     6661 cttacttttt tctttcattg ccctatcaat gtgtttaaat accacaaagg cggcagaaaa
     6721 aattgtttca actgcgggct atgcttccga aattgtcgca gcactaggaa aagctgataa
     6781 gttagtcggt gtggatacca cttcagttaa acctcaaacg ataatggaga aaaaacctaa
     6841 aatcggttat cgtcgccagc tttctgctga aggtattctc tctttaaccc cagatttgat
     6901 tattttagcc cctgatgccg gccctaacgc agttattgag caaattaaag ccagcggtat
     6961 tccattactt cacttaggag agaaaccgag cttagcaggg attcgtgagg atattcagca
     7021 aattgccaag gcgattggtg cagaatcaga ggcaaaacaa ttaacagaaa ccattctggc
     7081 agatgaaaaa aaattagccg acattcgtag ccaacattct actgacaaag aagtcttagt
     7141 gctgattgac actgcatcgc aaggtatctt tgggctaggg aaaaagagtg tgggtgatca
     7201 tttcttaacc ttgttaaatc taaacaatag tttcaatgct gagggcaata agccactcgc
     7261 caatgaggct ttagctgcaa gcaatgccga tgttatttta ttagcctccc gtaatcaggc
     7321 aaaagaatct gcaacgatca gtaaattagc agaatccagc ccacaatacg cccaactcac
     7381 aaatacacaa gcgggcaaaa aaggctgtgt ttttgcaatc aatattttag atgccttggg
     7441 ctttgggcct tacactgctc aatatgcaca ccaaattctc accactattc aaccttgttt
     7501 aaaataacgg atacgctttg aaactgcatt taaccttaat tttcacttta attatcagct
     7561 tagtgatttg tacccattta ggggcgatgt cattacccct atccgcttgg cactgcaaca
     7621 acgctgaatg cgaaagtgca gcctatattt tatggcaaat ccgcctgcct cgattactag
     7681 gggcattatt catcggggca gccctcgcta ttagcggagc aacaatgcaa gggctgtttc
     7741 gcaatccgtt agtagatccg gggattattg gtgtctcttc aggtgctgca ttggctgcgg
     7801 cattatatat tgtggtgatt cagccactac tgccggaatc aatcagctat tatgctctac
     7861 ctattgccgc ctttttaggg ggctggctgg tgacttttat gctctattta ctttctcaac
     7921 gtaacgggca actacatatc gccattatgc tattaattgg tattgctcta gctgcattta
     7981 gtggcgcttt aaccggcata ctgatttatg ttgccgatga taaccaactt cgtagtttaa
     8041 ccttttggag tatgggatct ttagcaggta tgaattggca aatggtggca atgatggctg
     8101 cggttgtctt catttgtagc ccacttattt ggcgggaaca ccgtgcgtta aacgccttaa
     8161 cattaggtga agaagatgca caatacattg gttttgacat tgcaaaagtc aaacgtcgtc
     8221 ttgtgtttta cgttgcctta ttaaccggca caagcgttgc ctgtgttggg gctatcggct
     8281 ttgtcgggct ggttattcca catttaatgc gtttactggg aggcagtaac caccgccatt
     8341 tattactcaa tagtttggtt tgcggtgcaa tcttattaat gattgcagat actcttgcca
     8401 gaaccctagc cgccccggca gaaatcccga ttggtatttt taccgcctta tttggtgcgc
     8461 cattccttgc agtaatggta tggaaaagtg ggaaacatat ctaatgagca atttattaac
     8521 ttgccaaaat ttaagcatcg ttcatcaaca aaaaaagttg ctggagattc aagaaattgc
     8581 ctttgaacaa ggaaaattta ccgccatcat cggtccaaac ggagccggca aaacaacctt
     8641 gctcaaagcc ttaagcggta ataatgcaca aactaacagt gccgtatttt atcaacatca
     8701 acgcttaaca gacttaaacg gatatgctct ggctcaaaaa cgggcaatgc tcagccaaca
     8761 gagccaaatt gcctttccct taaaagtaga agaattggta caactcggaa gagagccata
     8821 tcgccaaagt gaatttgcaa aatttaatga aaaaataacc gcttgggcta tggaaacaat
     8881 gtctttaacc gcattaaaag aaagaagtag catacaactt tccggtggag aacaacatcg
     8941 agcgcatatt gctcgagtgc tggctcaact cgttcctgaa ccgaatgccg atttaagcgg
     9001 aagctggctt ttgcttgatg aacccaccaa ccatttggat attcatcacc aatatcagct
     9061 tttttcctta ctacaaaagc tgaaacaaca aggcttaact atcattgcga ttttgcacga
     9121 tcctgcattg gcaataaatc aagccgatca tatcttaatg ctaaaaaatg gagaaaaatt
     9181 tgctgaaatg tccgctaaag ccattgcaga aagttgttta ttagatgagc tgtatgaaat
     9241 gaaaatgggc tgtcgctatt gccctcacag cgggcaatat tatctttgcc ctgcattagc
     9301 tgaaagttag ggtgcgatgc accctatcgt ttaatcttct gcatatccac aagcaggcag
     9361 tagtttaccg tctaaatacg ctttgccgtg ctgagaaact aatctacccg aacagaacca
     9421 ctcaatcacc aacggataat atcgatgttc ttgctcaacc actcgagcca ttacatcttc
     9481 aatctcatct tcaggataaa tcggcacttt agcttgtaat accaccgcac cagcatccac
     9541 ttcttcattc acgaaatgaa tagtcgtgcc gtgttcactt tccccagcct ctatcgctct
     9601 ttgataagtg tttagccctg ggtatttagg taataaggaa gggtgaatat tgagtatttt
     9661 gccagcaaaa tgttgcgtaa attcaggcgt taaaattttc atatagccgg ctaatacaat
     9721 taactcagct cctaactgct caatttgttc ggcaatcgca agatccattt ccacattgct
     9781 ttggtaatct ttacgagaaa atacaaatgt cgggattccc gcctgtttag ctcgaatcag
     9841 cccataggca tctgccttat tgcaaattac accgcaaatt tgaccgcttg tatccgcaga
     9901 tttttgcgca tcaatcattg cttgtaggtt agaaccattc cctgaaatta aaaccacaaa
     9961 ttttttcata ttacacaccg gctgaatatt catcgttctg gatattatgc cttgaatctc
    10021 aacatcagta caggaaagaa aaagggcgta ttggttaaaa taagccactt tcaagctaga
    10081 aacgcacctc tgcccctagt gtgtaagttc gacctctggc actatttgtg aaacgatagg
    10141 tgtctgtatc atagtcatac tcttctgcgg tggaagagtt atacgcattt aaggcatcaa
    10201 gataatttcg gttcatcaca ttttgtaccc cggcttttaa ggttaaatgt ttattcactt
    10261 tgtaatttgc atataaatcg ataatcaccg gaatattcgg taactgctcg tgaataatgt
    10321 tgccatcttc gtctaaatca ataccgacac gtttcgcttt gcctgtatat ttaaaaatgc
    10381 tagacagtgt taatttttta tccagcaatt tagcccctag ctccaaagtc gcataatctt
    10441 ttggtagctg ggtataagct tcagtgccaa atccataaag aatagtcgca ccttttgaag
    10501 tgggtgctgt gctttcagtt cgggtataac taccattgac aaaaaaagca tcggaatcgt
    10561 aagccagctc aacttcccaa cctcgatttt taaccttttc agggtgattc acataaattt
    10621 ggaactgtat agcattggta gcatcacttg aatttcgaca tactgttggc ccacaaccat
    10681 acaacgagag gttatacaaa tagtctttaa tgcgtgagtt aaaatatacc gcttttagcc
    10741 ccaagatatc attattgagt aatacattat cttgatgaat attaaaccca atctcatagg
    10801 ttttcgcact ttctggtttc aaaaacgggt tcacactcaa gccatttcga ctggtattaa
    10861 acatttcttg cacattaggc gaacgggagg tttgtttgta actgacaaaa ggttgaaacc
    10921 aatcagcaac ctgaaatgct aaagtggcag aaggattcaa cttataatgt tttttattaa
    10981 catcaaaccc aaaagaaggg aaacaataat ccatatcatc gctatcgcat tctggcttat
    11041 atccttttaa ctgatagcgt gaatagtgaa gacttgaatt taaagtgaca atgccgtatt
    11101 tccattcggt gtctaggtaa agggaatcaa tattttgctt acctgccggc ccaaaagcgg
    11161 cattgctaat cgctccatca ttattcttac tttccggtaa atcacgggaa tatttatttc
    11221 cctgactcaa aacggctggt gttattcaaa tcaaacatcc acattttgtt tttggttgag
    11281 gcatcttcta atgtccatag ttttgcatca tcgtggaaat cttgcttagt ttctgttact
    11341 gctgttagga atttcagatc aatccaatca gattgaggct ggtaactgga ttctaaggaa
    11401 taggtcacac tatcaatatt acgccccact aagctatttt tatattgacg tccggacaat
    11461 ttaaactctg aatcttgatt tggcttaaag gcaatttttc ccatataaga ttcagggtga
    11521 ttttttaacc cttgcatata agcagaatcc gttgccattg tgccattacc tcggcgataa
    11581 ttttgactga tactactttt gctaaaagcc aataaactgc caagagaacc actttccagc
    11641 acatcaactt ttccggctag tgcaagcatt ccgttttttc ccagatcatt attgccataa
    11701 ttaaaacggc ttaatacgcc gactttatgt tgttctagta gaatatcttc gacatcaatc
    11761 gttttaaaat tagcactacc ggctaaagca ttcaatccgg ctgcaccatt gttgtagcct
    11821 ttcgcaatat caactcgggt taaaaaattt tgatcaatta aagtgctgaa ttggctcatt
    11881 ggcccgttac catcagtgtg gaacttacca cctccgtttg ctgatgtacc aagctgtgtt
    11941 tgtggcacgc catcaactaa ggtatttacc cgaccaagac cactcatatt acgaatattc
    12001 acactcacgc ccccttgccc cgggtcaatt tgggtatagc cacctgcgac agaacggaca
    12061 atactatcta aactttgcat tgcattcaca ttcattgctc gagaactcac cgagccggct
    12121 ttactgaaat tctcaaaatt agggttttcc acagtgccat aaaccgtcac agtatcaagc
    12181 tccgcaatat ttttcgcctt ttcctctgcc aatgcagttt gcgatcctcc catacacaaa
    12241 accggaaaca aaatgcgtgt gattatatta aatttcataa ttctccttat tgagtgaccc
    12301 aaagataatc tgcctgtgaa gaagtaattt cttctctctc tgttttaagc attagcaccg
    12361 ctactttttt tgttcggtca tattccgcaa tatgcaacgg aacgccaaga tctttgcgaa
    12421 cgtggtggtt acctgcaatc agcaagctag tcgataacgc attttttact aatttttctg
    12481 ccattctgcg atcacggaat tgttgtactg ccaccatatt ttccatcggg taaggcattc
    12541 cgtgattcat tgacagcaac agttgtgcga tcttttgctt aacttcagag gaagtggata
    12601 cagaaccttt taatggctcc gccccattta ataaaatttc aacctcttta tctgttaagt
    12661 tagtggctat aacacgacta tttgaaccta agctctctac cactaaatct tgatacatct
    12721 tccaatccca tttttgccat tgaattgcct ctcttaaatc cgtggctgat aaattaaccg
    12781 gtcggttaaa ctgaatgcga tcaatcgcag gctgctgatc cacatttaac atctccaacg
    12841 caattaaatg taacttggct ttcttcgtaa gttgattaaa taaatacaat tcaatttggt
    12901 ggtgaattaa ctgagtatgc tcctcaccaa ctaaaacgta atcttgagaa gatagacggg
    12961 aaagtagttc agtaaatgaa atcgtttgat tggtattcag atcaataatt tggccgatag
    13021 agtccacgtt ttgttcatta agggattgcg ttgaaaccga gcagcctaaa agaaaaaaag
    13081 ccataaaaag gctagctaaa aaagaggaaa tacgaggtaa cataacaaaa aataaattaa
    13141 tgagaattat tatcattatg ttatgtaaaa aaagaaaaat caatccattt tcagaaaaca
    13201 gataaagaaa atgattgata aaacaaaaag cggtaatatt tctaaaaaat attaccgctt
    13261 gaaaaagtgt aaagttgaga ttatgcgtac actggtaaac gtttacaaat gtctaaaact
    13321 ttcgctttag tctctgcaat cacctgttcg tgattatcct taccaatgct atctagcaca
    13381 tcgcacatcc agccggctaa tgctgtcacc tctgcttctt taaaaccacg gcgggtcaca
    13441 gaaggcgtac ccacacgaat acctgaagtc acgaatggtt tttgagggtc gttcggcacc
    13501 gagtttttat tcacggtaat gttcgcagct cctaatgcag catctgccgc tttgccggtt
    13561 aaaccttgtt ttacaaagct aacaaggaat aaatggtttt ccgtaccgtt tgaaaccaca
    13621 tcaaaaccac gttgcttaaa tacctcaacc attgcttttg cattttttac aacttgttgc
    13681 tgatatgcct taaattccgg ctctaaagct tctttaaaac aaaccgcttt tgccgcaata
    13741 acgtgcatca atggtccacc ttgaccagca gggaatacgg cagattgcag tttcttatac
    13801 atttcttcat cgccagatgc agataaaatc aaaccacctc gagggccggc taaggtttta
    13861 tgggttgttg tcgtcacgac gtgagcgtga ggaagcggat ttggataaac tcccgccgca
    13921 attaagcctg caacgtgagc catatccaca aataaatatg ccccaacttc atcggcaatc
    13981 tcacgcattt ttgcccaatc cataacttga gaataagcag agaaaccagc aacaatcatt
    14041 ttcggtttca cttcttgggc ttgtttgcgt aatgcttcat aatcaattaa gccttcatca
    14101 gtaatgccgt attgttctgc tttataaatt ttaccagaga agctaactga agcaccgtgg
    14161 gttaaatgcc cgccgtgcgc taaactcata cctaaaatag tgtcacccgg attaagcaac
    14221 gccatatata cggctgcatt cgcttgtgag cctgaatgcg gttgaacgtt ggcataatct
    14281 gccccgaaca atgctttcgc acggtcaatc gcaagttgtt ctacaatatc cacatactca
    14341 caacccccat agtaacgttt acccggataa ccttctgcat atttattggt taattgtgag
    14401 ccttgagcct gcattacacg tgggcttgca tagttttcag atgcaattaa ttcaatatgt
    14461 tcttcttgac ggcgatcttc atcttgaatg gcttgccata atactgcatc gtaatcagcg
    14521 atattcatat cacgttttaa catttttgga ctcctaataa gcttaagtaa acgtttgcgt
    14581 aagttgattt cacgttattt tacgcatttt ttagtttcat agatagtatt atttatataa
    14641 aaattaactt cgagtgaaag aaaaacaagc ggtcacattt tgt
//
LOCUS       DANLXF010000048        14432 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.048, whole genome shotgun sequence.
ACCESSION   DANLXF010000048 DANLXF010000000
VERSION     DANLXF010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14432
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.048"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            112..306
                     /locus_tag="PXD66_000877"
     CDS             112..306
                     /locus_tag="PXD66_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HDL6319199.1"
                     /translation="MKPATFYFAIFLLNLTACTPKVQLETPSEGITINMNVVVDHRID
                     VKMDEKSRAVITKSENKTKN"
     gene            complement(303..776)
                     /gene="folK"
                     /locus_tag="PXD66_000878"
     CDS             complement(303..776)
                     /gene="folK"
                     /locus_tag="PXD66_000878"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDL6319200.1"
                     /translation="MQTVYIALGSNLENPLEQLKQAVEKLKKFANEFEISPFYGSKPV
                     GPQDQPDYVNAVAKFSTDLSPTALLDKLQSIENEQGRVRVRRWGERTLDLDIILYGNQ
                     QIQTERLNVPHIEMKNREFVIVPMFDLTPDLVLPTGEKLADIYAQFKDHQMIIFS"
     gene            complement(979..2310)
                     /gene="pcnB"
                     /locus_tag="PXD66_000879"
     CDS             complement(979..2310)
                     /gene="pcnB"
                     /locus_tag="PXD66_000879"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HDL6319201.1"
                     /translation="MLHKKFAVNASQYRISPKDFPKNAISIVDKLQIAGYEAYIVGGC
                     IRDLLLEKKPKDFDIATNARPEEIQRIFGRQCRLIGRRFRLAHIVYGREIFEVATFRA
                     GHESHRNDKISKVSDEGMLLRDNVYGTLREDAERRDFTVNALYYDIKRNLIFDFFNGI
                     EDLRAGKLSLIGEPTIRYQEDPVRMLRAVRFMAKLDMFLDKATASPIRDMAVLLTNIP
                     AARLFDESLKLLQTGAGLKTYQLLREYELWQFLFPVLPAFFTAKGDSYAERMITKALN
                     STDERIADKLPVNPAFLFAALLWYPLREKMEQLKNEGGFNSYDAMMLAANDVLAESCN
                     AIALHRRHTSVIRDIWALQFQMMKRSGNRPQQTLEHVKFRAGFDLLVMRAEIEGGDLV
                     ELSAWWHEYQFSNEGQRTELLKSVRHLGNVPSEKKKTRRRRTFKKKPKVNG"
     gene            complement(2555..3001)
                     /gene="dksA"
                     /locus_tag="PXD66_000880"
     CDS             complement(2555..3001)
                     /gene="dksA"
                     /locus_tag="PXD66_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HDL6319202.1"
                     /translation="MTQAAKTSLGLLALAGVEPYELKKDEEYMNEAQREHFTKILKAW
                     HSQIMEEAERTKSQLQEDMTNFADPNDRASQEEEFSLELRNRDRERKLLKKIEQTLYK
                     IQEDEYGYCETCGVEIGIKRLEARPTADLCIDCKTLAEIREKQLGL"
     gene            complement(3251..4204)
                     /gene="thiB"
                     /locus_tag="PXD66_000881"
     CDS             complement(3251..4204)
                     /gene="thiB"
                     /locus_tag="PXD66_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HDL6319203.1"
                     /translation="MKFILSSLALLSASVYANQPTLTVYTYSSFASEYGPAPKLEQNF
                     EKECGCDVKFVPLDDSVTMFNRLRLEGAKTKADVVVGIDNFLQPEAEKSGLFAEFEAK
                     TKLETQQKIFFPYDTGSYAFIYDKEKLKNPPKSLKELVERQDLKVIYQDPRTSSVGRG
                     FLMWVNQVYGDEAPTAWKTLAKHTVTVGKGWSETYGAFLKGESDLVLSYTTSPLYHKW
                     HENTDKYVAAPFEEGHLVQVENAAIVKSSQQKALANQFLEFLHKPESQHIISYHNVMN
                     PVLTDGVDPAFAAMPTYKTIEFTPPTTETIQKWLADWSKSW"
     regulatory      complement(4248..4391)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4675..5661
                     /locus_tag="PXD66_000882"
     CDS             4675..5661
                     /locus_tag="PXD66_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDL6319204.1"
                     /translation="MSQLIRTLKGHIRDEIIKKGGWVNAHAHADRAFTMTPEKITVYQ
                     NANLQQKWDLVDEVKRNSSVEDYYRRFSQAIELMISQGVTAFGTFVDIDQVCEDRAII
                     AAHKAREVYKNDIILKFANQTLKGVIEPNAKKWFDIGSEMVDMIGGLPYRDELDYGKG
                     LEAMDILMDKAKSLGKMLHVHVDQFNSPKEKETEQLCDKSIEHGMQGRVVAIHGISIG
                     SHPKEYRKMLYQKMRDSQMMVIACPMAWIDSGRKEDLQPFHNALTPADELIPEGITVA
                     IGTDNICDYMVPLCEGDMWQELSLLSAGCRFTNLEEMANIASINGRKVLGLV"
     gene            complement(5715..6134)
                     /locus_tag="PXD66_000883"
     CDS             complement(5715..6134)
                     /locus_tag="PXD66_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HDL6319205.1"
                     /translation="MLGNILGSLASSVLSGNNQSTATQLIQNLLASQGGIEGLIAKFQ
                     QGGLDDVLRTWISSDEDNAPVSGEQISNVFGQENIQNVAQEAGVDAADASDLLAQFLP
                     KIVDTLTPNGNVEDLKNFDANDLLAQAAKGILGNLFK"
     gene            complement(6212..6967)
                     /gene="kdsB"
                     /locus_tag="PXD66_000884"
     CDS             complement(6212..6967)
                     /gene="kdsB"
                     /locus_tag="PXD66_000884"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HDL6319206.1"
                     /translation="MKFTVIIPARYASSRLPRKPLLDIAGKPMIQHVWEKAQLAGASR
                     VIIATDHPEIEALAQSFGAEVCMTSEKHNSGTERLAEVIEKMQIADSEIIVNIQGDEP
                     LIPPVIVTQVAQNLDAYQVNMATLGVKLATREELFNPNAVKVVADKNGMALYFSRASI
                     PFARDSFPDCDDSFVAAQGYLRHIGIYAYRAGFVKQYVAWQPTQLEQLESLEQLRALW
                     HGEKIHIEPAKEAPQVGVDTPEDLERVRAILAR"
     gene            complement(6977..7930)
                     /gene="trxB"
                     /locus_tag="PXD66_000885"
     CDS             complement(6977..7930)
                     /gene="trxB"
                     /locus_tag="PXD66_000885"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDL6319207.1"
                     /translation="MTTKHTKLLILGSGPAGYTAAVYAARANLNPVLVTGMQQGGQLT
                     TTDEIENWPGEFEQITGTELMNKMLKHAEKFETEIVFDHIHSVDLSKRPFTLNGDLYT
                     FTCDALIIATGASAKYLGLPSEEHFKGKGVSACATCDGFFYRNKPVAVVGGGNTAVEE
                     ALYLANLASEVHLIHRRDSFRAEKILLGRLQKRIEEGKIILHTDRTVDEVLGDNMGVT
                     GLRLKSTKEELSEEIKVDGFFVAIGHAPNTAMFEGQLELENGYIKVKSGLEGNATATS
                     VEGVFAAGDVMDHNYRQAITSAGTGCMAALDAERFLDAQEA"
     gene            8097..9167
                     /gene="dhaK"
                     /locus_tag="PXD66_000886"
     CDS             8097..9167
                     /gene="dhaK"
                     /locus_tag="PXD66_000886"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012073177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDL6319208.1"
                     /translation="MKKLINSIETVLDEQLSGFVKAHSDLTLNTEPVYIYRTTPSNQP
                     KVALISGGGSGHEPMHAGFIGKGMLDGACPGAIFTSPTPDQMFECGLAVDRGEGVLLI
                     IKNYTGDVLNFETATELLADSGVKVATVLVDDDVAVKDSLYTAGRRGVANTVLIEKLL
                     GAAADKGYNLSELAKLGHQLNNNGHSIGIALSACTVPAAGKPSFTLAENEMEFGVGIH
                     GEPGIERRPFENLDSTVKQMFETLIAHGDYQRKIRHWNTQTQQWDEIEESKVALKKGD
                     RVIALVNNLGAVPLSELYAVYDALEKECQTFGLSIERSLVGSYCTSLDMQGISITLLK
                     VDEDILKLWDAPVNTPAMRWGE"
     gene            9188..9814
                     /gene="dhaL"
                     /locus_tag="PXD66_000887"
     CDS             9188..9814
                     /gene="dhaL"
                     /locus_tag="PXD66_000887"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HDL6319209.1"
                     /translation="MTISKQQILKWLENCQQVMTEKRDYLTELDTVIGDGDHGLNMQR
                     GFSKALEKAHTVQDKDIGTLLKTVGMTLLSQVGGASGPLYGSFFIKAAQGATGKEQLS
                     FNELLALLQQGAEAIVQRGHAQVGDKTMCDLWLPLLEDLAQADSTQPIAQILLQAVEK
                     AENFANQTAPLMAKKGRASYLGERSIGHKDPGATSSYYIFKALAEALQ"
     gene            9824..10231
                     /gene="dhaM"
                     /locus_tag="PXD66_000888"
     CDS             9824..10231
                     /gene="dhaM"
                     /locus_tag="PXD66_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HDL6319210.1"
                     /translation="MVNLVIVSHSAKLAEGVGEILAQMAQGGCKIALAGGIEDTSNPI
                     GTDAVKIMQAIESVFSPDGVIVFVDLGSAILSTQTAIDLLEPELAEKVIISYAPLVEG
                     AFAAVVAASAGDSLEAVLNEANEAAALKQEQAN"
     gene            complement(10271..11116)
                     /locus_tag="PXD66_000889"
     CDS             complement(10271..11116)
                     /locus_tag="PXD66_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone YbbN"
                     /protein_id="HDL6319211.1"
                     /translation="MDYFVNVNEQNFSEVWAAAAQVPTVFHFISPREVASLEMDALLR
                     RISDENPNQFLLATVNCDEQQALAAQFRIQQIPTLYLFAGGQPIDAIQGAVSEQELRI
                     RLANILPKEEELKFQQALELLQDQRYTEALPLLKSAWEMTDKKNSDFALLYAETYIAM
                     KSVEEAREILKAIPIQDRDSRWHGLQAQIELLEQASESPELQQLQADYANSKTPEIAI
                     KLANQLHQAHKNEEALTLLFDWLKSDLNVANGEVKSQFLAILSALGTDPIVPKFRRQL
                     YSLLY"
     gene            complement(11160..11486)
                     /gene="glpE"
                     /locus_tag="PXD66_000890"
     CDS             complement(11160..11486)
                     /gene="glpE"
                     /locus_tag="PXD66_000890"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HDL6319212.1"
                     /translation="METTFKEISPQQAWELIENEGAVLADVRDARRYVYSHPQDAFHL
                     TNQSYGKFLDEVDYDEPVVVICYHGVSSQSTAQFLIEQGFENVYSVKGGFEAWERNGL
                     PIETSY"
     gene            11637..12095
                     /gene="exbB"
                     /locus_tag="PXD66_000891"
     CDS             11637..12095
                     /gene="exbB"
                     /locus_tag="PXD66_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HDL6319213.1"
                     /translation="MEQLFQFLEHHTDFVILGLLGLLSVICLWKAIERIMFYSKVDIS
                     KYDTLEELDIDLTHNLTTISTIGSNAPYIGLLGTVVGILLTFYHLGQSGGDIDAASIM
                     VNLSLALKATAVGILVAIIAMVFYNGFGRKVEENKLKWLALQSKKPHQAV"
     gene            12132..12569
                     /gene="exbD"
                     /locus_tag="PXD66_000892"
     CDS             12132..12569
                     /gene="exbD"
                     /locus_tag="PXD66_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HDL6319214.1"
                     /translation="MKKFDEINIIPFIDIMLVLLAIVLVTASFISQGKIQVNVPKAST
                     TQPMKADDLAKLLTITENNEFFFNDQAITKEQLIAEVATWDKSQKVSLKVDGAVAFEK
                     FVELTDILSANEIKNVAIITKKETAPAPSSTPGSPAQVPAVAP"
     gene            12585..13496
                     /locus_tag="PXD66_000893"
     CDS             12585..13496
                     /locus_tag="PXD66_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HDL6319215.1"
                     /translation="MKKKHSRIGLLASLAIHTALIGGTFAWLHNHKVEKIAEQNSLSM
                     EMVAALLEQPQVAVAPDPITEEVKEIEPEKAEVPPEPKLTPEPTPEPEAIPDPTLNPK
                     KEKPKEEKRKERHHHKHKHRHKEKREELQEQEKPKDKPKERPKHHHKHIKALERGPEI
                     KQGIVAKAIPNAAEGEKVVAGVVGGQKNGSPNSTSTTGSANTGASSGNASELAAYKVA
                     LQRALQRKANNSYPQREKMMRKTGTVTIKFTITSSGSITNVSVLNSSGNSNLDNAAVR
                     SAEGLSMPPPPAGFPPTVTVPVRFALE"
     gene            complement(13565..>14432)
                     /gene="cysZ"
                     /locus_tag="PXD66_000894"
     CDS             complement(13565..>14432)
                     /gene="cysZ"
                     /locus_tag="PXD66_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HDL6319216.1"
                     /translation="SEPQNQVGLGFHYFIYGWRLLLQRQFLPFVIFPILINTVLMVGL
                     IWGFFANIGGLLDMLLPSWLEWLSFVLIPLIFLMILVGFYFAFTTLANFVAAPFNALL
                     AEKVELQLTGEPLGDMSFGAMLKDVPRMLKREWQKMMYSIPRLIALFVLGFLPVVGQT
                     VAPVLTFIFGAWLIAIQYCDYPFDNHKISFQRMRNALSQSRTLNFTFGALVSLFTMIP
                     FVNLVVMPVAVCGATALWVKEYRNFFLNNQTGEFSKADYTFTKVNSTSLSTETRTGEV
                     SPNVRNGDIRKQ"
BASE COUNT         4331 a         3103 c         2886 g         4112 t
ORIGIN      
        1 agaaaatctg tttgatttgt ggcacttaat taactatggc tcacaatttg aacaaaaatt
       61 agaaaattac ctctatcaaa aactggaacg atgataaaga atggaataaa tatgaaaccg
      121 gccacatttt attttgcaat atttttactg aatttgaccg cttgtacgcc gaaagtacag
      181 cttgaaacgc caagtgaagg cattacgatt aatatgaatg tggtcgttga tcaccgcatt
      241 gatgtgaaga tggacgagaa atcccgagcg gtgatcacca aaagtgagaa caaaacgaaa
      301 aattagctga agataatcat ttgatgatct ttaaattgtg cataaatatc ggctaatttt
      361 tcccctgtcg gtagtactaa atcaggcgtt aaatcaaaca tcggcacaat cacaaattcc
      421 cgatttttca tctcgatatg cggcacgttc aaacgctcgg tttgaatttg ctgattgccg
      481 tacaaaataa tatccagatc taaagttctc tccccccaac ggcgtacacg cactcttccc
      541 tgctcatttt caatgctttg caatttatcc agcaatgcag tgggcgatag gtcggtacta
      601 aattttgcta ccgcattgac ataatcaggc tgatcttgcg gtccgacagg ctttgagccg
      661 tagaacgggc tgatttcaaa ctcatttgca aattttttta atttttcgac cgcttgttta
      721 agctgctcaa gcggattctc aaggtttgat cctaaggcga tatagacggt ttgcataaaa
      781 tatacttaca aatataacaa taaactctct ccccttgtgg gagagagacc gcttatgagc
      841 gttcagcgaa ataagcagag agaggggaca agcggtaact tttattaaat tttttacaca
      901 tttcccctct ccctgatttt tcttctgaac gaagaaaaat cgctccctct cccacaaggg
      961 gagagggaaa tatattaatt atccatttac tttcggtttt ttcttaaagg ttctacggcg
     1021 gcgtgttttc tttttctcgc taggcacatt gcctaaatga cgaaccgatt tcagcaattc
     1081 agtacgctga ccttcattgc taaattggta ttcgtgccac caagcggaaa gttcgaccaa
     1141 atcgccgcct tcaatttctg cacgcatcac taataaatca aaccctgcac ggaatttgac
     1201 gtgttcaagg gtttgttgcg gtcggttgcc acttcgtttc atcatctgaa attgcaatgc
     1261 ccaaatatct cgaatgactg aagtgtgtct gcggtgcaac gcaatcgcgt tgcaactttc
     1321 cgccagcaca tcatttgctg ccagcatcat tgcatcgtag ctgttgaagc caccttcgtt
     1381 tttaagctgc tccattttct cccggagcgg ataccaaagt agtgcagcaa ataaaaacgc
     1441 aggatttacc ggtaatttat cggcaattcg ctcatcagtt gaatttaacg ctttggtaat
     1501 cattctttcg gcataggaat cacctttggc agtaaaaaat gccggcaata ccggaaacaa
     1561 gaattgccat agttcatatt cacgcaaaag ctgataagtt ttcagccctg caccggtttg
     1621 taataatttg agtgattcgt caaacaaacg ggctgccgga atgttggtta acaacaccgc
     1681 catatcccga attggggagg ccgtggcttt atccaagaac atatccaact tcgccataaa
     1741 acgaacggct cgcaacattc tcaccggatc ttcttggtaa cgaatagtag gctctccgat
     1801 aagcgaaagt ttacctgctc gtaagtcttc aataccgttg aagaaatcaa aaataagatt
     1861 gcgttttata tcgtaatata aggcgtttac ggtgaaatca cggcgctctg catcttcacg
     1921 gagcgtgccg taaacattat cacgcagtag cattccttca tcgctgactt tagaaatttt
     1981 gtcattgcga tgagattcgt gtcctgcacg gaaagtcgcc acttcaaaaa tttcacgccc
     2041 gtaaacaatg tgtgccaaac ggaaacgacg tccgattaat cggcattgcc taccaaaaat
     2101 acgctgaatt tcttcaggtc tcgcattagt cgcaatatcg aaatctttag gctttttctc
     2161 caagagtaga tctcgaatac agccacccac aatataagct tcataaccgg caatctgcaa
     2221 tttatcgaca atcgaaatcg catttttagg aaaatccttc gggctaattc tatattggct
     2281 ggcattcacc gcaaatttct tgtgtaacaa atgggaggga ggattatctt gttgtttctt
     2341 cccacctcta taagtctttt ggtccgttaa gttcggggct gggcgtttag aacgggaagt
     2401 tttgggttct ctttccgctc ttttttcact ctctaccttt tcatttttct gagccgactt
     2461 tttaggcttg gaaaataatg atttaatata attaaaaata atacacctct aaatagtaaa
     2521 tgcctcttaa aaaagaggca ccctgtaaag ttgattataa gcctagctgc ttttctcgaa
     2581 tttcagctaa agttttacag tcaatacata aatcagctgt tgggcgtgct tctaaacgtt
     2641 taatcccaat ttctacgcca caggtttcgc aataaccata ttcatcttct tggattttat
     2701 acaaggtttg ctcaattttt ttcaataatt tgcgttcacg atcacggtta cgaagctcta
     2761 aactgaactc ttcttcttga ctggctcggt cgttcggatc ggcaaagtta gtcatatctt
     2821 cttgaagctg gcttttggta cgctccgctt cttccataat ttgactgtgc cacgctttca
     2881 gaattttggt gaaatgctca cgttgagctt cattcatata ttcttcgtct tttttaagct
     2941 cataaggctc aacgcctgct aatgctaata aaccgagtga ggttttagcc gcttgagtca
     3001 tagatattta ctccttgttc cctgtttatt ggaagtttta ttgttaaaat tattgcccga
     3061 atacagacta acttccttgt aaccgcacta tacgaaaaaa tcaacatctt cgggttttaa
     3121 ataatggcgg ctataaataa cacaaaaacg actcattaat caaatgaatt tgcaaaaatt
     3181 ttgcattcaa attgatttga ttgcacaaaa aaatagccga acatccattc ggctattttt
     3241 caaacacttt ttaccagctc tttgaccaat cggctaacca tttttggata gtttcagtag
     3301 tcggtggcgt aaattcaatg gttttatagg ttggcattgc cgcaaaagcc ggatcaacgc
     3361 cgtcggttaa aaccggattc attacgttgt ggtaagaaat aatatgttgc gattccggtt
     3421 tgtgcaaaaa ttcaagaaat tgatttgcca atgctttttg ttggctgctt ttgacaatag
     3481 ccgcattttc tacttgcacc aaatgccctt cctcaaacgg agcagccaca tatttatcgg
     3541 tgttttcgtg ccacttatga tataaaggcg atgtagtata gcttagtacc aaatccgact
     3601 cgccttttaa gaacgctcca taagtttcag accaaccttt gcctaccgtt accgtatgtt
     3661 ttgccaatgt tttccaagca gttggagctt catcgccata cacttgattt acccacatta
     3721 agaagccccg cccaacgctg ctggtacgtg ggtcttggta aatcactttt aaatcttggc
     3781 gttccaccaa ctcttttaaa ctttttggtg gatttttcag cttctcttta tcgtaaataa
     3841 aagcataaga acccgtgtca taagggaaga aaattttctg ttgggtttct aatttggtct
     3901 ttgcttcaaa ctcggcaaac aagccacttt tttcggcttc aggttgcagg aaattatcaa
     3961 taccgaccac cacatcagct ttggtttttg ctccctccaa acgcaagcgg ttgaacatcg
     4021 ttacgctatc gtcaagcgga acaaatttga catcacagcc acactctttc tcaaaattct
     4081 gctctaattt aggagcaggt ccatattccg aagcaaacga gctgtaagtg taaacggtta
     4141 aggtcggttg attggcataa acacttgcac ttaataacgc aagtgaagag aggataaatt
     4201 tcataacaat tccttctaaa ttaaatgaat agaaggattt aagaaaggca agttgctatc
     4261 tcaaatccct acgctgatat taatcagatc aggttctatg ggtatttctc agccacaagc
     4321 ggttaaattt cggcaaaaat ttgcaaattt ttacgcaaaa acaaccgctt gttgcacccc
     4381 aatgagaacg gcttcattgt aaaaagtttg caggcagatt tcaaccaaag caaacgtttg
     4441 ctatttgtat taaaaatccg tatactaccg ccctagtcca aacaaggaac tagaaatgaa
     4501 ttgcacaaca tcggttaaat caaattccca acagctacgc ttcaacttta gaggcgtagc
     4561 tgtttttcta ttcatttcaa accattcaaa atcgctgtta tagcgatttt ttttcgcacg
     4621 cttgcaatta ccgcaagcgg ttgagccaca ttaacagaga gaaaaggaat aaaaatgagc
     4681 caattgattc gtaccctaaa aggacatatt cgtgatgaaa tcatcaaaaa aggcggttgg
     4741 gtaaatgcac acgcccacgc agatcgtgca tttacgatga cgcctgaaaa aatcaccgtt
     4801 taccaaaatg cgaatctaca gcaaaaatgg gatttggtcg atgaggtaaa acgtaattca
     4861 agcgtagaag attactaccg ccgcttttcc caagcgattg aattgatgat ttcacaaggg
     4921 gtaacggcat ttggtacttt tgtggatatc gaccaagtat gtgaagacag agccattatt
     4981 gccgctcaca aagcccgtga agtatataaa aacgacatca tcttgaaatt tgctaaccaa
     5041 acgttgaaag gcgtgattga acccaatgcg aaaaagtggt tcgatatcgg ctcggaaatg
     5101 gtggatatga ttggtggctt gccttaccgt gacgaactgg attacggcaa aggcttggaa
     5161 gcaatggata ttttaatgga taaagccaaa tcgctcggca aaatgctcca cgtccacgtt
     5221 gatcaattta actcacctaa agaaaaagag accgaacaac tgtgtgataa atcgattgag
     5281 catggtatgc aagggcgagt ggttgccatt cacgggattt ccatcggctc gcacccgaaa
     5341 gaatatcgaa aaatgcttta ccaaaaaatg cgtgatagcc aaatgatggt cattgcctgt
     5401 ccgatggcgt ggattgatag cggacgtaag gaagatttac aaccgttcca taacgcttta
     5461 acccctgctg atgaactcat tcctgaaggc attactgttg ctatcggcac agataatatc
     5521 tgtgattata tggtaccgct ttgcgagggc gatatgtggc aagaactcag ccttttatca
     5581 gcaggctgtc gttttactaa cctagaagaa atggcaaata ttgccagtat caatggtcga
     5641 aaagtactgg gtttagtcta actgaataaa cgaaaggcga accctaggtt cgcctttaat
     5701 aacagcataa ccgattattt aaacaagtta cccaaaatcc cttttgccgc ttgagctaat
     5761 aagtcatttg catcaaagtt ttttaagtct tcaacattac cattcggggt taaagtatcc
     5821 acgatttttg gcaagaactg ggctaataaa tcgctcgcat cagcagcatc aacacccgct
     5881 tcttgagcca cattttgaat gttttcttgc ccgaacacat tactgatttg ttcgcccgaa
     5941 acaggtgcat tgtcttcatc gctgctgatc caagtgcgta acacatcatc taatccgcct
     6001 tgttggaatt tggcaatcag cccttcaatc ccaccttgtg aggcgagtaa attttgaatt
     6061 aattgagttg cagttgattg gttattgcca ctcaatactg aagaagccaa tgaacctaaa
     6121 atatttccta acataatttc tcctatgcaa gcggtacaat tttgcgaaaa ttttgcaaaa
     6181 aaacagcaaa cttcgaccgc ttgtaatcac tttaacgagc taaaatggct cgcactcttt
     6241 ccaaatcttc cggcgtatcc acccccactt gcggggcttc tttcgccggt tcgatatgaa
     6301 ttttttcacc gtgccataag gctcttagtt gctcaaggga ctcaagctgt tctaattgtg
     6361 tcggctgcca cgccacatat tgtttcacaa aaccggctcg gtaagcgtaa atgccgatat
     6421 ggcgtaaata accttgagcg gcaacaaaac tgtcatcaca atccggaaaa ctgtctctgg
     6481 caaacggaat gctggctcgg gaaaaataaa gtgccattcc gtttttgtct gccaccactt
     6541 tcaccgcatt tgggttaaat agctcttctc gggtcgctaa tttcacaccg agtgttgcca
     6601 tattgacttg ataagcatct aaattttgag ctacctgagt aacaattacc ggtggaatca
     6661 gcggttcatc accctgaata ttcacaataa tttcgctgtc ggcaatctgc attttttcga
     6721 tcacttccgc caaacgctca gtgccggaat tgtgtttttc cgaagtcata cagacttccg
     6781 ccccaaaaga ttgagcgagg gcttcaattt cagggtgatc ggtggcaata atcacacggc
     6841 ttgcaccggc aagctgagct ttttcccaaa cgtgttgaat catcggtttg cctgcaatat
     6901 ccaacaacgg cttacgaggc aaacggctgg aagcgtaacg agcaggaata attacagtga
     6961 atttcatagg ctatccttat gcttcttgag catctaagaa acgttctgca tccagggctg
     7021 ccatacaacc tgtgccggca gaggtaatcg cttggcgata gttatgatcc atcacatcac
     7081 cggcagcaaa tacgccttcc acagaagtgg cagtagcatt gccttccaaa cctgatttca
     7141 ctttaatgta gccgttttca agttctaatt gaccttcaaa cattgccgta ttcggtgcgt
     7201 gcccaatcgc cacaaagaaa ccatcaacct taatttcttc gctcaattct tctttggttg
     7261 attttaaacg taagccggta acgcccatat tatcgcctaa cacctcatca accgtacggt
     7321 ctgtgtgcag aataattttg ccttcttcaa tacgtttttg cagacgacca agcaagattt
     7381 tttcagcacg gaagctatca cggcggtgaa ttaaatgcac ttcacttgcc aaatttgcca
     7441 aataaagtgc ttcttccaca gcagtattcc caccaccgac taccgcaacc ggtttattgc
     7501 gatagaagaa accatcacaa gttgcacaag ccgacacgcc tttgcctttg aaatgctctt
     7561 cagaaggcaa acctaaatat ttcgccgacg caccggtggc aataattaac gcatcacagg
     7621 tgaaagtgta taaatctccg tttagcgtga atgggcgttt agataaatcc actgaatgaa
     7681 tatgatcaaa cacgatttca gtttcaaatt tttcagcgtg cttgagcatt ttgttcatta
     7741 actctgtgcc ggtaatttgt tcaaattccc ccggccagtt ctcgatttca tcggttgtag
     7801 tgagctgtcc gccttgttgc ataccggtga ctaaaaccgg gtttagattg gcacgggcgg
     7861 cataaacagc agcggtataa ccggcaggcc ctgagcctaa aattaaaagt ttggtgtgtt
     7921 tagtggtcat aatgaatcct ctgaataaat attaatgttt attttagcct aaatttcagt
     7981 tcaactctat tttcgttttc gctcaacttt tttcaatttt gtgacaaagt taacagaaat
     8041 ccgactgtgt ttcttgtaaa ttgtgacagt ctaattcatt taaaaggggc aaaaagatga
     8101 aaaaattaat taattcaata gaaaccgtct tagatgagca actttctggt tttgtgaaag
     8161 cacattctga tttaacactt aacacagaac ctgtctatat ttaccgcaca acgccaagta
     8221 atcaacctaa agtagccctt atttccggtg gtgggagtgg gcacgagcca atgcacgccg
     8281 gttttatcgg taaaggaatg ttagacggag cttgccccgg agcaatcttt acatctccaa
     8341 cgccggatca aatgtttgaa tgcggtttag cggttgatcg tggcgagggt gtattgctaa
     8401 ttattaaaaa ctataccggt gatgtgctga attttgaaac agccaccgag ctacttgcag
     8461 atagcggtgt aaaagtcgca accgtgttgg ttgatgatga tgtggcagtc aaagacagtt
     8521 tatatactgc cggccgccgc ggtgttgcaa atacagtatt aattgaaaag ctgttaggag
     8581 ctgcagcgga taaaggctat aacttatccg aattagccaa attagggcat cagttaaaca
     8641 ataacgggca ttctatcgga attgccttat ctgcttgtac tgtgccggct gccggtaagc
     8701 catcatttac tttagctgaa aatgagatgg aatttggggt aggtattcac ggagaaccgg
     8761 gcattgaacg tcgccccttt gaaaatctgg atagtaccgt gaaacaaatg tttgagacct
     8821 taattgctca cggcgattat caacgtaaaa ttcgccattg gaatacacaa acccaacaat
     8881 gggacgaaat cgaagaaagc aaagtggcat tgaaaaaagg tgatcgtgtt attgctttag
     8941 ttaataactt aggagcagtg ccactatccg aactttatgc tgtttatgat gcgttagaaa
     9001 aagagtgcca aactttcggc ttaagcattg aacgcagtct tgttggatca tattgcacct
     9061 cgctggacat gcaaggtatc tctatcacat tactgaaagt tgatgaggat attctcaaac
     9121 tatgggatgc tccggtaaat acaccggcaa tgcgttgggg tgaataatta gattgaaagg
     9181 ataagttatg acaatatcaa aacaacagat cttaaaatgg cttgaaaatt gccaacaggt
     9241 aatgactgaa aaacgggatt atttaaccga attggatacc gttattggcg atggcgatca
     9301 cggtttaaat atgcagcgtg gatttagtaa agcacttgaa aaagcccata ccgtgcaaga
     9361 taaagatatc ggcaccctgc ttaaaaccgt tggaatgaca ttattatcgc aagtaggggg
     9421 agcgagcggc cctctctatg gttcattttt tattaaagcg gctcaaggtg ctacaggcaa
     9481 agagcaactg agctttaacg agttactggc acttctacag caaggagcag aagctatcgt
     9541 acaacgtgga cacgcacaag ttggcgataa aacgatgtgt gatttatggc tgccattact
     9601 cgaagactta gctcaagccg atagcactca accaattgct caaatcttat tacaagcggt
     9661 cgaaaaagca gaaaattttg caaatcaaac cgctccatta atggcgaaaa aagggcgtgc
     9721 cagttacttg ggcgaacgca gtatcgggca taaagatccg ggagcaacgt ccagctacta
     9781 tatttttaaa gccttagctg aagcattaca gtaggagggg taaatggtta atttagtgat
     9841 tgtttcacat agtgctaaac tggcggaagg cgtgggggaa attttggctc aaatggcaca
     9901 ggggggctgt aaaatcgccc ttgccggcgg cattgaggat acctcaaatc ctatcggcac
     9961 tgatgcggta aaaataatgc aagcgattga atcggtattt agcccagacg gtgtgattgt
    10021 gtttgtcgat ttaggcagtg caattttaag tacccaaacc gccatagatt tactggagcc
    10081 ggaacttgcc gaaaaagtga tcattagcta cgctccttta gttgaaggtg cttttgctgc
    10141 tgtagttgca gcctctgccg gcgatagttt agaagcggta ttaaatgaag cgaacgaagc
    10201 cgctgctctt aagcaggaac aagccaatta gtaaaaatgc cgtatcgaaa aatacggcat
    10261 tttagtttta ttagtacaat agtgaataaa gctgtcttct aaatttcggc acaatcgggt
    10321 ctgtacccag tgcagataag attgccagaa attggctttt tacctcgccg ttcgctacat
    10381 ttaaatccga ttttaaccaa tcaaaaagta acgtgagtgc ttcttcattt ttatgagctt
    10441 ggtgtagctg atttgccaat ttaatcgcaa tttcaggggt tttgctgttc gcataatccg
    10501 cttgaagctg ttgtaattcg ggcgattcgc tggcttgttc caataattcg atttgggctt
    10561 gcaaaccgtg ccaacggctg tctctgtctt gaatcgggat cgctttaagg atttcacgag
    10621 cctcttccac gcttttcatt gcaatgtaag tttcggcata gagcaaagcg aaatcgctgt
    10681 ttttcttatc ggtcatttcc cacgctgatt tcagcagcgg caaggcttcg gtataacgct
    10741 gatcttgcaa taattccaag gcttgttgaa atttcagctc ttcctctttc ggtaaaatat
    10801 ttgccaaccg aatccgtaat tcctgctcac ttactgcacc ttgaattgca tcaatcggct
    10861 gaccgccggc aaacaaatag agcgttggaa tttgttgaat gcgaaattga gcagccaatg
    10921 cttgttgctc atcacaattt accgtcgcca ataaaaattg gttagggttt tcatccgaaa
    10981 tacgacgtag taaggcatcc atttccaatg aagccacttc ccttggcgaa ataaaatgaa
    11041 acaccgtcgg cacttgtgcc gctgctgccc atacttcact aaaattctgc tcgtttacat
    11101 ttacaaagta gtccatacaa aaccttctta agctaaaaat tactttttat attgtagcat
    11161 taataagaag tttcaatagg tagcccgttt ctctcccacg cttcaaaacc accttttaca
    11221 ctataaacat tctcaaaccc ttgttcgatt aaaaactgtg ctgtactttg gctactcact
    11281 ccgtgatagc aaatcacaac caccggttcg tcataatcga cctcgtctaa aaacttacca
    11341 taactctggt tggttaaatg aaacgcatcc tgtgggtggc tgtaaacata acggcgggca
    11401 tcacgcacat cggcaagcac cgcaccttcg ttttcgatta attcccaagc ctgttgcggg
    11461 ctaatttctt taaatgtagt ttccataatc actttccctc aaaaaacgtg caactatacc
    11521 ataaaattct aaaaagaatg gctattattc caagttgaaa atttgatgtt tgtcagatat
    11581 aattgcaaac tattttcaaa tgcactgatt taaaactacc acagtttgag gattttatgg
    11641 agcaattatt tcaattttta gaacatcata ctgactttgt tatattagga ttactaggct
    11701 tattaagcgt aatctgcctt tggaaagcta ttgaacgtat tatgttttat tccaaagtag
    11761 atatttcaaa atacgatacc ctcgaagaat tagacatcga tttaacccac aatttaacta
    11821 ccatttcgac tatcggctct aatgcccctt atatcggctt attaggaacc gttgtcggta
    11881 ttcttttaac tttctatcat ttaggtcaat caggtggtga tattgatgcg gcctctatta
    11941 tggtaaacct ttctttagct ctaaaagcca ctgctgtcgg tatcttagtg gcaattattg
    12001 caatggtttt ctacaatggt tttggtcgta aagtggaaga aaacaaactg aaatggttgg
    12061 cattacaatc aaaaaagcct catcaagcgg tgtgatttag tccatttttt gcaaattaat
    12121 aaggcagaac aatgaaaaaa ttcgatgaaa tcaacatcat tccatttatt gatattatgt
    12181 tggtattact ggctatcgtg ttagttaccg cctctttcat ttctcagggt aaaatccaag
    12241 taaatgtacc gaaagcaagt acaacacagc caatgaaagc agacgattta gcgaagcttt
    12301 taaccattac cgaaaataat gagtttttct ttaatgatca agcaataact aaagagcaat
    12361 taattgctga agttgccact tgggataaaa gccaaaaagt gtcactcaaa gttgatggtg
    12421 cagtcgcctt cgaaaaattt gtggaattaa ccgatatcct ttctgcaaat gaaattaaaa
    12481 atgtagcaat cattactaaa aaagaaactg ctccagcacc ttcaagcaca cctggtagcc
    12541 ctgcacaagt gccagcagtc gcaccttaaa tagggtaaac aggtatgaaa aaaaaacatt
    12601 ctcgtatcgg cttattggca tctttggcta ttcacactgc cttaattggt ggcacttttg
    12661 cttggttaca taaccacaaa gtggaaaaaa ttgccgagca aaactctctt tctatggaaa
    12721 tggtggcagc attattagag caacctcagg ttgccgttgc tccggatcct attaccgaag
    12781 aggtcaaaga aatcgaaccg gagaaggcag aagtaccacc tgaacctaag cttactccgg
    12841 agccaactcc agagccggag gcaattccag atcctacgct aaatccgaaa aaggaaaaac
    12901 cgaaagaaga aaagcgtaaa gagcgacacc accataaaca taagcatcgt cacaaagaga
    12961 aaagggaaga gctacaagag caagaaaaac cgaaagataa gcctaaagag cgcccgaaac
    13021 atcatcacaa gcatattaaa gcattagagc gcgggccaga gataaaacaa ggtattgtcg
    13081 ctaaagcgat tccaaatgcg gctgaaggtg aaaaagtggt tgcgggggtt gttggagggc
    13141 aaaaaaacgg ttcaccaaat tcaacctcaa ctacaggttc agcaaatact ggggctagca
    13201 gtggtaatgc cagtgaactt gctgcttata aagtcgcatt acagcgagca ttacagcgta
    13261 aggcgaataa ctcttaccct cagcgtgaaa aaatgatgcg gaaaaccggt acagttacga
    13321 ttaaatttac cattacatcg tccggttcaa ttacaaatgt atccgtactc aattcatcgg
    13381 gaaatagtaa tttagataac gctgcggtac gcagtgcaga aggtttgagt atgcctccgc
    13441 caccggcagg cttccctcct acggtaacag taccggtaag atttgccctt gaataaaaac
    13501 agccctcaaa agcaatgtac ttttgagggc tttttattgc aaaaaattga ctaaaaatga
    13561 ccgcttactg cttgcgaata tcgccatttc tcacattcgg actcacttca cccgtacgag
    13621 tttctgtgct taacgaggta gaatttactt tcgtaaaagt ataatccgct ttgctgaatt
    13681 cacctgtttg attatttaag aagaaattgc ggtactcttt tacccataat gccgttgcgc
    13741 cacaaactgc cacaggcata actactaaat tcacaaatgg aatcatcgtg aataggctca
    13801 ctaaagcacc aaaggtaaaa tttaaagtgc gagactgcga caacgcattt cgcatacgct
    13861 gaaaactgat tttatggtta tcaaacggat agtcacaata ttgaatggcg atcaaccaag
    13921 caccgaagat aaaagtcagc acaggtgcta cggtttgccc aaccacaggc aaaaagccta
    13981 atacaaaaag agcaatcagc cttggaatgc tatacatcat tttttgccat tctcgtttca
    14041 gcattcgagg cacatctttt aacattgcac cgaatgacat atcgcccaaa ggctcgccgg
    14101 taagttgcag ttcgactttt tctgctaata acgcattaaa gggagcagca acaaagttag
    14161 ccaaggtagt aaaagcaaaa taaaatccca ctaatatcat caaaaaaatc agtggaatca
    14221 gtacaaagct aagccattcc agccagcttg gcagtaacat atccaacaag ccaccgatat
    14281 tggcaaaaaa tccccaaatc agccctacca tcagcacggt attaataaga attgggaaaa
    14341 tcacaaaagg caaaaattga cgttgtaaca ataaccgcca gccatatata aaataatgaa
    14401 agcctaatcc gacttgattt tgcggctcag aa
//
LOCUS       DANLXF010000049        13839 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.049, whole genome shotgun sequence.
ACCESSION   DANLXF010000049 DANLXF010000000
VERSION     DANLXF010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13839
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.049"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            10..792
                     /locus_tag="PXD66_000895"
     CDS             10..792
                     /locus_tag="PXD66_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HDL6319217.1"
                     /translation="MNCNTPTDITFSFDGFRRRGGLTDHHTDGFGIAFFEGKGVRVFR
                     DNRPGASSPMAEAISQYQIKSLNVIAHIRKATRGDVNLENTHPFIRELWGENWVFAHN
                     GTVEGVGVCEECHYQPIGTTDSEAAFCRIVSELKEMYPAKPSEKEIFDAVVQITSRIA
                     EHGVFNFVLSNGHWMIARCTTNLHYVCRKAPFGKALRDDDVSIDFSQYTKITDKVTII
                     ATQPLTKNENWTKMKNGGYVFFKDGDVVYEIEGTLPEAQPHV"
     gene            complement(827..1720)
                     /gene="ispA"
                     /locus_tag="PXD66_000896"
     CDS             complement(827..1720)
                     /gene="ispA"
                     /locus_tag="PXD66_000896"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HDL6319218.1"
                     /translation="MSYLLATDLTHLQQRINHFLAEKLKTTDSVGSPLVEAMAYAVLL
                     GGKRVRPFLIYATGRMLGVPLEKLDHSAAAMEAIHAYSLVHDDLPAMDDDRLRRGKPT
                     CHIAFDEATAILAGDALQSFAFELLATDPHLTDREKVVQVTELATASGAKGMCLGQSL
                     DLIAENKAVSLEELELIHRNKTGALILSSVMMGLNLSEYSQNVAIKQPLVNYAKAIGL
                     AFQVQDDILDVIGETDKIGKIVGSDEHLNKSTYPKLLGLDGAKQKAEMLYQTALHSLE
                     QLPFDTTALKELANFIVKREN"
     gene            complement(1726..1956)
                     /gene="xseB"
                     /locus_tag="PXD66_000897"
     CDS             complement(1726..1956)
                     /gene="xseB"
                     /locus_tag="PXD66_000897"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDL6319219.1"
                     /translation="MAKKPTQDFESTLKELEAIVTHLESGDLPLEEALNEFETAIKLV
                     QQGQERLQKAEQRIQILLNKNDTAPLSDYERE"
     gene            2081..2869
                     /locus_tag="PXD66_000898"
     CDS             2081..2869
                     /locus_tag="PXD66_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HDL6319220.1"
                     /translation="MQVTELNIYPIKSTQAIQVSQAFVEPKGLNFDREFMLAEPNGKF
                     ITARKDPELYRLAAFPIATGVMITHTSGQKCVALYQDFVEEQSSEVWGTHFNAKVAAE
                     AVNQWLSEIFGRAVQLRWLGSQSQRIVANFADHPMSFADSNPVSLMSLKSLEQVQQWS
                     PIPLEMARFRPNIVIDGNVAFEEEQWQQVQIGEVLFTKAALCTRCILITRDLNTLELD
                     PKSEPFRTLKEKHCDEKGKPVFGIHLVPQNSGVIKVGDPVIIQL"
     gene            2902..4851
                     /locus_tag="PXD66_000899"
     CDS             2902..4851
                     /locus_tag="PXD66_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL6319221.1"
                     /translation="MYQRSQSRTVLFPILLFVGINLVILTLSRLVLGVWQSDRVSAVD
                     GWIPLILQGIRIDISSLCWLFGLPALFSVLCFHKNVLGSIWQTVLRIWLTVGSVFILF
                     MEVATPNFIETFDLRPNRLFIEYLVHPREVFTMLINGHLWAVICGIGIAMISLIIYWK
                     LSGWATKNLHFPSWKMRPLLALLVAAIVFVGGRSSFAHRGINPAMVAFSSDPLVNSLV
                     LNSGYSVLYAVQQLKDEDKSSEQYGKMSVEEMFNIVKQVRNRPASDYISDEIPTLTQN
                     KATYQGKPKNIVIILEESLGAQFVGSLGGKNLTPNLDKLSEQGWYFTNLYATGTRSVR
                     GIEAVTAGFTPTPARSVVKLTKSQTNFFSIAELLKRQGYHTSFIYGGEKHFDNMASFF
                     YGNGFQQIIDEKDYKNPKFTATWGVSDEDLFDKANETFNQLHKSGKPFFSLVFSSSNH
                     DPFEFPDGKIELYEQPKQTRHNAAKYADYAIGHFFELAKKSEYWQDTVFLVIADHDSR
                     AVGEHLVPIQHFHIPALLLGEHIQPRTDNRLVSQLDMPTTLLSVAGISGQYPMIGYDL
                     TQDIDPNRAFMQYDATQAMMRGNDVVVMRPNLPIEGFSYDKANYKLTPKEQPESFKKE
                     ALAHALLGSYLYKNQLYKLPEETKQ"
     gene            complement(4922..5839)
                     /gene="nagK"
                     /locus_tag="PXD66_000900"
     CDS             complement(4922..5839)
                     /gene="nagK"
                     /locus_tag="PXD66_000900"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005708433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HDL6319222.1"
                     /translation="MAIYYGLDVGGTKIELAAFNEKLEKLHSERVPTPQTNYQDWLTA
                     IKTLVETADTKFDEKGTVGLGIPGFVNRETGIAEITNIRVADGKTILKDLAECLGREV
                     RAENDANCFALSEAWDESNKPYSTVLGLIIGTGFGGGFVFDGKIHSGRIGMAGEVGHM
                     QLNYHALKLLDWDKAPIYECGCGNQACLDTYISGRGFELLFNDLVGEKISAKAIIERF
                     YQQETKVVAFVEKYIELMAISISNLITILDPDMIVFGGGLSNFDYIYQALPKALPKHL
                     MRSAKVPVIKKAIHGDSGGTRGAAALFLK"
     gene            complement(5852..6214)
                     /locus_tag="PXD66_000901"
     CDS             complement(5852..6214)
                     /locus_tag="PXD66_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319223.1"
                     /translation="MYQVFIAEPKDPNLEKGLLKHFIKSYLTQEELDFLHKILTNLPN
                     IRPFWGDTGYKYIVIDNVYFLYEVSESETKVILLGTKFNNKRKTFNFANTKTQQKSMK
                     KWINDLAIYKPHKINQSQ"
     gene            complement(6222..6383)
                     /locus_tag="PXD66_000902"
     CDS             complement(6222..6383)
                     /locus_tag="PXD66_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319224.1"
                     /translation="MEMSIEKIAELLSDKEKDDYPFSIEERQFAAQASADTWEAILNN
                     TIQPFDFKK"
     gene            complement(6452..7174)
                     /locus_tag="PXD66_000903"
     CDS             complement(6452..7174)
                     /locus_tag="PXD66_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDL6319225.1"
                     /translation="MFKKFSLYTLAMLLIPVVTWLANWHWSLAIQYAEFDFDYFLYLI
                     TETGSVPYAIITCVVFALLLARVVRKRYSLIFVITVCVASMVATQAIKTAMKSTFQEP
                     RPFVTALFQDKTDDFYQLTREQRGLAVMEKIGANQNFVTAHQAKEVGYSFPSGHTIFA
                     VSWLLLVVGFCRNMRGQAVVLAHFFAISWAALMLISRLRLGMHYPIDLFTSSLIAFLI
                     NLALFVWFVPWLESKKWFAKKP"
     gene            complement(7235..7549)
                     /gene="yhbY"
                     /locus_tag="PXD66_000904"
     CDS             complement(7235..7549)
                     /gene="yhbY"
                     /locus_tag="PXD66_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010786380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDL6319226.1"
                     /translation="MSITLTTKQKQFLKGLAHHLSPVVMLGGNGLTEGVLAEIDNALD
                     HHELIKVKVAGADRETKQLIIDAILRETKAAEVQTIGHILVLYRQSEEKKISLPKATK
                     AI"
     gene            7676..8434
                     /locus_tag="PXD66_000905"
     CDS             7676..8434
                     /locus_tag="PXD66_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL6319227.1"
                     /translation="MPTLSVAMIVKNEAQDLRACLDTVKDWVDEIVILDSGSTDETPQ
                     IAAEYNARFYTNTDWQGFGKQRQLAQQYVTSDYVLWLDADERVTAELKQSILQAVKND
                     EKNTVYKISRLSEVFGKQIRHSGWYPDCVVRLYRTEFAKYGDELVHEKVHYPKEAKIQ
                     KLQGDLLHFTYKDIHHYLVKSASYAKAWAIQRANAGKTASLFDGVTHAIGCFVKMYLL
                     KAGFLDGKQGFLLAVLSAHSTFVKYADLWNRTRK"
     gene            complement(8499..9539)
                     /locus_tag="PXD66_000906"
     CDS             complement(8499..9539)
                     /locus_tag="PXD66_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDL6319228.1"
                     /translation="MKKLLVIRNDKIGDFMVCFPAFAMLKQSMPNVEITALVPSYTAP
                     LAELCPSIDKIIIDTANKKDKNEFNRILQAVKNEQFDAVICFVSDWHNAKLTWQSGIK
                     YRLAPATKLFQFLYNHRLTQRRSQSAKAESEYNLDLARAFLSKHNIPIVEPHTPYLSV
                     EQQTVQAQKAKLCEQLNITTVKKWVFVHSSTGGSANNLSIEQYAELIQGILAEFDCYV
                     VLTAGKGESEKAQQLAQQINHNNVVVYDKNEGLQDFTHSLACADLFIAGSTGPLHISG
                     ALNIPTIGFYPSRLSAIPRRWKPINDANKHIAFCPPSDKASQMNLTLISISEALKTIV
                     PFIRRMWNTPSL"
     gene            9723..11054
                     /gene="argG"
                     /locus_tag="PXD66_000907"
     CDS             9723..11054
                     /gene="argG"
                     /locus_tag="PXD66_000907"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005722481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDL6319229.1"
                     /translation="MSATILEALPLGQKVGIAFSGGLDTSAALLWMRKKGAVPYAYTA
                     NLGQPDEDDYNAIPKKAMEYGAENARLIDCRQQLAHEGIAAIQCGAFHISTGGIPYFN
                     TTPLGRAVTGTMLVAAMKEDDVNIWGDGSTFKGNDIERFYRYGLLTNPKLKIYKPWLD
                     QLFIDELGGRLEMSKFLIENGFDYKMSVEKAYSTDSNMLGATHEAKDLEELSTGMKIV
                     KPIMGVAFWDESVEIKAETVSVTFEEGVPVALNGKRIDDAVELIMEANRIGGRHGLGM
                     SDQIENRIIEAKSRGIYEAPGMALLHIAYERLVTGIHNEDTIEQYRINGLRLGRLLYQ
                     GRWFDPQALMLRETAQRWVAKAITGTVTLELRRGNDFTILNTESPNLTYEAERLSMEK
                     VEDAPFTPADRIGQLTMRNLDIVDTRGKLAVYTDAGLLAANNSVIPQLGKK"
     gene            11147..11608
                     /gene="rnhA"
                     /locus_tag="PXD66_000908"
     CDS             11147..11608
                     /gene="rnhA"
                     /locus_tag="PXD66_000908"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HDL6319230.1"
                     /translation="MKLVEIFTDGSCLGNPGKGGIGILLRYNGYEKTVSKGYFQTTNN
                     RMELRAVIEALAMLKEPCKVQLNSDSQYMKNGIQKWIFNWKKNDWKTSDKKPVKNKDL
                     WVALDQEIQRHQIEWSWVKGHSGHRENEICDELAKQGANNPTLDDVGYIAD"
     gene            11754..12257
                     /locus_tag="PXD66_000909"
     CDS             11754..12257
                     /locus_tag="PXD66_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HDL6319231.1"
                     /translation="MVDLIVIGIIAFSVLISLWRGFTNEALSLLGWVIAFFVASSFYP
                     YLSGYLTQINSVYLQNSEFLRNGIAAAILFILTLIVCGVINGLLGKVIDLSGMSITDR
                     ILGGAFGALRGILIVSAILFFLDTFTQASQTELWKSSLLIPHFDFIVKWFFEQLQANS
                     SFLNTTK"
     gene            12272..13789
                     /gene="purF"
                     /locus_tag="PXD66_000910"
     CDS             12272..13789
                     /gene="purF"
                     /locus_tag="PXD66_000910"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDL6319232.1"
                     /translation="MCGIVGIIGNSPVNQAIYDGLTLLQHRGQDAAGIVTIDDENRFR
                     LRKANGLVSDVFQQEHMVRLQGNVGIGHVRYPTAGSSSVSEAQPFYVNSPFGITLVHN
                     GNLTNNAELKARLYNEARRHVNTNSDSESLLNIFAYFLDLYSTQHLSPDNIFETVRKT
                     NDSIRGAYACIAMIIGHGMVAFRDPFGIRPLVLGKREIEGKTEYMFASESVALDVVGF
                     EFVRDVLPGEAIYVTFDGQLHSQICADNPKLNPCIFEYVYFARPDSVIDGVSVYSARV
                     HMGELLGEKIKREWGRIIDDIDVVIPIPETSNDIAVRIANMLYKPYRQGFVKNRYVAR
                     TFIMPGQAQRKSSVRRKLNAIASEFKGKSVLLVDDSIVRGTTSEQIVEMARAAGAKRV
                     YFASAAPEIRYPNVYGIDMPTCEELVAYDRSVEEVAQMIGVDKLIFQDLEALYKSIQL
                     ENPTIHRFDDSVFTGEYITGDVDKCYLDSIARSRNDKAKAEAAKQATNLEIHNER"
BASE COUNT         4018 a         2626 c         2977 g         4218 t
ORIGIN      
        1 ttacttggaa tgaattgtaa tacgccaacc gatattactt tttcttttga tggatttcgc
       61 cgtcggggtg ggctgacgga tcaccatact gatgggtttg gaattgcctt ttttgaaggc
      121 aaaggggttc gggtttttcg tgataaccgc ccgggagcat catcgccgat ggcagaagca
      181 atcagccaat atcaaattaa atcgctcaat gttattgcac atattcgtaa agctacgcgt
      241 ggtgatgtga atttggaaaa tacccacccc ttcattcgtg aattatgggg cgagaactgg
      301 gtgtttgccc ataacggcac agtggaaggt gttggggtat gcgaggagtg ccactaccag
      361 ccgattggta caaccgattc cgaagcggca ttttgccgta ttgtgtcgga attaaaagag
      421 atgtatccgg ctaaaccaag cgaaaaagag atttttgatg ccgtggtgca aatcacctca
      481 agaattgcgg aacacggggt atttaatttt gttttatcca acggtcattg gatgattgcc
      541 cgttgtacaa ctaatctgca ttacgtttgc cgtaaagcac cgtttggcaa agccttgcgt
      601 gatgatgatg tgtcgattga ttttagtcaa tataccaaaa ttaccgataa agtgacgatt
      661 attgccactc agccacttac caaaaatgaa aactggacca aaatgaaaaa cggtggctat
      721 gttttcttta aagacggcga tgtggtatat gaaattgaag gcacattgcc ggaagctcag
      781 cctcacgttt aatcactaaa aaacctagct tcggctaggt tttttcttag ttttcacgct
      841 taacaataaa attagctaac tcttttaatg cggtggtatc gaaaggcaac tgttccagcg
      901 aatggagagc ggtttgataa agcatttcgg ctttttgttt tgccccgtct aagcccaaca
      961 atttcgggta tgtgctttta tttaagtgtt catctgaacc cactattttg ccgattttat
     1021 ctgtttcgcc aatgacatct aaaatatcat cttgcacctg aaaggctaag ccaatcgctt
     1081 tggcatagtt tacaagcggt tgttttatgg ctacattttg cgaatattcg gaaaggttta
     1141 atcccatcat cacggatgat agaatcaatg ccccggtttt gttgcgatga attaattcaa
     1201 gctcttccaa gctcaccgct ttattttccg caatcaaatc caaactttgc cctaagcaca
     1261 ttccttttgc accggaagcg gttgccaatt ctgtaacttg aacaactttt tctcggtcgg
     1321 tcaaatgcgg atcggttgcc agcagttcaa acgcaaaact ttgtaacgca tcaccggcta
     1381 aaatagcggt agcttcatca aaggcaatat ggcaggttgg cttgccacgg cgtaatctat
     1441 catcgtccat tgccggtaaa tcatcgtgaa ccagcgagta agcgtgaata gcctccattg
     1501 ccgccgcact atgatctaat ttttccaacg gcacaccaag cattctgccg gtggcataga
     1561 ttaaaaacgg tcgcacccgt ttaccgccta gcaataccgc ataagccatt gcttctacta
     1621 aaggcgaacc aacgctatcc gttgttttta atttttcagc gagaaaatgg ttaatccgtt
     1681 gttgcaggtg agtcagatcg gttgctaata gatagctcat tgtgattatt ctctttcgta
     1741 atcgctaagt ggggcagtat cgtttttatt taataagatt tgaatacgtt gctcggcttt
     1801 ttgtaaccgt tcttgccctt gttgcactaa tttgattgca gtttcaaatt cgttgagggc
     1861 ttcttcaagc ggtaaatcac cggattccaa atgggtaacg attgcttcta attctttaag
     1921 tgtgctttca aaatcttgag tgggtttctt tgccattgtt gctgacctat tgctgtaaaa
     1981 gttgctcaag tttaccgttc tcacaaggga tttgcaaatt ccttttactt ttcgaccgct
     2041 tgcaggtatt gttatcctat ttccaaatgg aggattttct atgcaagtta ccgagctcaa
     2101 tatttacccg ataaaatcaa ctcaagctat tcaagtttca caggcatttg ttgaaccgaa
     2161 aggcttaaat tttgaccgtg aatttatgct agccgagcct aacggcaaat ttattactgc
     2221 ccgtaaagat ccggaattat accgtctagc ggctttcccg attgcgacag gggtaatgat
     2281 tacccataca agcggtcaaa aatgcgttgc actttaccaa gattttgtgg aagagcagag
     2341 tagtgaagtg tggggaacac attttaatgc caaagtggcg gcagaagcgg tcaatcaatg
     2401 gctgagtgaa atttttggta gggctgtgca actacgttgg cttggctcgc aaagccaacg
     2461 aattgttgcg aattttgccg atcacccaat gagttttgca gatagcaacc cggtgagttt
     2521 gatgtctcta aaatcgcttg agcaagtgca acaatggtcg ccgattccac tcgaaatggc
     2581 tcgttttcgc ccgaatattg tgattgatgg caatgtggca tttgaagaag agcagtggca
     2641 acaagttcaa attggcgaag tgctatttac caaagcggca ttatgtactc gttgcatttt
     2701 gattacacgg gatttaaaca cgcttgagtt agaccccaaa tcggagccgt ttagaacctt
     2761 aaaagaaaag cattgcgatg aaaaaggtaa gccggtgttc ggtattcacc ttgtgccaca
     2821 aaacagtggt gtgataaaag taggcgatcc tgtcattatt caattataat atgccgatat
     2881 ttttttaaaa ggagattcaa aatgtaccaa cgcagtcaat caagaactgt gctatttcca
     2941 atcctactat ttgttgggat taatttagtt atcctcacct tatcccgttt agtgttaggc
     3001 gtttggcaat ctgatcgagt atccgctgtt gatggatgga taccattaat actacaaggt
     3061 attcgcattg atatatcttc gctatgttgg ctatttggct taccggcatt atttagcgtg
     3121 ttgtgttttc ataaaaatgt actgggttcg atttggcaga ctgttttaag aatttggcta
     3181 actgtcggta gtgtatttat tctttttatg gaagtcgcca caccgaattt tattgaaacc
     3241 tttgaccttc gcccaaatcg tcttttcatt gagtatttgg tccatccgag agaagtgttc
     3301 acaatgctga ttaacggaca tttatgggct gttatttgtg ggattggcat tgcgatgatt
     3361 tctctcatca tttattggaa attatcgggt tgggcaacca aaaatttgca tttcccgagc
     3421 tggaaaatgc gtccgctact ggctttattg gttgctgcaa tagtttttgt tggtgggcgt
     3481 tccagttttg cccatcgagg gattaatccg gcaatggtgg cattttcaag cgatccgctt
     3541 gtcaattcac tggtgctaaa ttcaggttat tccgtgcttt atgcggttca gcaattgaaa
     3601 gatgaagata aatcttccga acagtatgga aaaatgtctg tggaggaaat gttcaacatt
     3661 gttaagcagg ttcgtaatcg accagcttca gattatattt cagatgaaat tcccaccctc
     3721 acgcaaaata aagcgactta tcaaggaaag cctaaaaaca ttgtgattat cttagaagaa
     3781 agtttagggg ctcaatttgt cggttcgcta ggagggaaaa atctcactcc aaatttagat
     3841 aaactttcag aacaaggctg gtattttacc aatctttatg caacaggtac tcgttcggtg
     3901 agaggaattg aagcggtaac ggctggtttt acaccaacac cggcaagatc tgtggtaaaa
     3961 ctgacgaaaa gccaaactaa cttttttagt attgccgaat tattaaaacg tcaaggatac
     4021 catacttcct ttatttatgg tggggagaag cattttgata atatggcgag ctttttctac
     4081 ggtaatggtt tccagcaaat tatcgatgaa aaagattata aaaaccctaa atttaccgca
     4141 acttggggcg tgagtgatga agatctgttt gataaggcaa atgaaacttt taatcaactg
     4201 cacaaatcag gtaaaccgtt ttttagtttg gtattcagtt ccagtaacca cgatccgttt
     4261 gaatttccgg acggcaaaat tgaactctat gaacaaccga agcagactcg ccataatgcg
     4321 gcaaaatatg ccgattatgc tatcgggcat ttctttgagt tagcgaaaaa atcagagtac
     4381 tggcaagata cggtgttttt agtgattgca gatcacgatt caagagccgt tggcgagcat
     4441 ttagtgccaa ttcaacactt ccatattccg gcacttttat taggcgaaca cattcagcca
     4501 agaacggata accgtttagt gagtcagctc gatatgccga caacattact ttctgtggcg
     4561 ggaataagtg gtcaatatcc gatgataggg tatgatttaa cgcaagacat tgacccaaac
     4621 cgtgccttta tgcaatatga tgcgacacag gcgatgatgc ggggaaatga tgtggtggta
     4681 atgcgaccaa atttaccgat agaaggattt agctacgata aagctaacta taaactcacc
     4741 cctaaagaac agccggaatc gtttaaaaaa gaggcgttag cacacgcatt gcttggcagt
     4801 tatttatata aaaatcagtt atataaatta ccggaagaaa ctaagcagta aggcaaatta
     4861 aaagagaggg ctgcaatatt tttattaaaa atgaccgctt gccctctctc aaatttgttc
     4921 attactttaa gaataatgct gctgcaccac gagtgccacc tgagtcgccg tgaattgctt
     4981 ttttgatgac cggcactttc gctgaacgca ttaagtgttt tggtaatgct tttggtagag
     5041 cttggtagat ataatcaaag ttggataaac cgccaccgaa tacaatcata tccggatcta
     5101 aaatcgtaat cagattgctg atactgattg ccattagctc aatatatttt tcgacaaatg
     5161 ccaccacttt ggtttcttgt tggtagaagc gttcgataat cgctttcgcc gagatttttt
     5221 ctcctactaa atcattaaac agtaactcaa aaccccgccc tgaaatatag gtgtctaaac
     5281 aggcttggtt gccacaacca cattcataaa ttggggcttt atcccagtcg agcaatttaa
     5341 gtgcgtggta gttaagttgc atatgcccaa cttcgcctgc cattccgatt ctgcctgaat
     5401 gaattttacc gtcaaacaca aaaccaccgc caaaaccggt gccgataatc aaccctaata
     5461 cggtggaata aggcttatta ctttcgtccc acgcttcaga cagggcaaag cagttagcat
     5521 cgttttcggc acgcacttca cgccctaaac attcggctaa atctttcaga atcgttttgc
     5581 cgtcagccac tcgaatattg gtaatttccg caatgccggt ttcacggttt acaaagcccg
     5641 gaatgcccag cccgacagtg cctttttcgt caaattttgt atcggcagtc tcaaccagcg
     5701 ttttaattgc cgtcagccaa tcttgataat ttgtctgcgg tgtcggcaca cgttcgctgt
     5761 gtagtttttc tagtttttca ttaaaagcgg ctaattcaat ttttgtgccg cccacatcta
     5821 agccataata aattgccata attacgctct cttattgcga ttgattgatt ttatgcggtt
     5881 tataaattgc taaatcatta atccatttct tcattgattt ttgctgggtt ttcgtattcg
     5941 caaaattaaa agttttccgt ttattattaa atttggtgcc aagtagaatg actttcgttt
     6001 cactttcgga aacttcatac aagaagtaaa cattatcaat gacgatatat ttgtaaccgg
     6061 tatcgcccca aaaaggtctg atgttgggta aattagtgag aattttatgc aggaaatcga
     6121 gttcttcttg agtaagataa gacttaatga agtgtttcaa taagcctttc tctaagtttg
     6181 ggtcttttgg ttctgcaata aaaacttgat acatagcaag cctatttttt gaagtcaaaa
     6241 ggttgaatag tgttatttaa gattgcctcc caagtatcag cactagcctg ggcggcaaat
     6301 tgtctttcct caatactaaa cggatagtca tctttttctt tatcgcttaa tagttcagct
     6361 attttttcta tcgacatttc cattctaagc tccttatttt aggtacttat tcaataattt
     6421 gtattgtata ttgatttggc taaattgcca attaaggctt tttagcaaac cattttttac
     6481 tttccaacca aggcacaaac cacacaaata aggctaaatt gattagaaac gcaattaaac
     6541 tactggtgaa taaatcaatc gggtagtgca tacctaaacg caagcggcta atcagcatta
     6601 acgccgccca actgattgca aaaaaatgag ccaaaacgac cgcttgtcct cgcatattac
     6661 ggcagaaacc taccaccaat aatagccaag aaaccgcaaa aatagtgtgg cctgatggga
     6721 aagaatagcc cacttctttg gcttggtgag cggtaacaaa attttgattt gcaccgattt
     6781 tttccattac ggcaagccca cgttgctcgc gtgttaattg ataaaaatcg tctgttttat
     6841 cttggaaaag agcagtgaca aatggacgag gctcttgaaa ggttgatttc atcgctgttt
     6901 tgattgcttg agtggctacc attgaagcga cacacacggt aatcacaaaa attaaagagt
     6961 agcgtttgcg aaccaccctc gccagtaata aggcgaaaac cacacaggtg atgatggcat
     7021 aaggtacgga gccggtttcg gtgattaaat ataaaaagta gtcgaaatca aattcagcgt
     7081 attgaattgc cagagaccag tgccaatttg ccagccaagt caccacagga atgagcaaca
     7141 ttgcaagggt ataaagagag aattttttaa acatagaaag tcctataaaa aaccgcttga
     7201 atacaagcgg ttgaatttaa acatattttt gcaattagat cgctttagtt gcttttggca
     7261 agctgatttt tttctcttcg ctttggcggt aaagcactaa aatgtgccca atggtttgta
     7321 cttcagccgc tttagtttca cgcagaatgg catcaataat taattgtttg gtttcacgat
     7381 cagccccggc aactttaact ttaatcagtt cgtggtggtc taaggcgtta tcgatttcag
     7441 ctaacacgcc ttctgttaag ccgttacccc ccagcatcac cactgggctt aaatgatgag
     7501 ccaagccttt taggaattgt ttttgtttgg ttgttaatgt aattgacatt ctttttccgt
     7561 taatttgttt tgttgataaa atttggcgta gaaaatagca caaaaaccac tatttttaaa
     7621 gtaaaatgca acgaatagca gaaaaacaca cgacaaaaag agagaaggat aaactatgcc
     7681 gacattaagt gttgcgatga ttgtcaaaaa tgaagctcaa gatttacggg cttgccttga
     7741 cacagtaaaa gattgggtag atgaaattgt gattttagat agtggcagta ctgatgaaac
     7801 accacaaatt gctgctgaat acaacgcaag gttctacact aacaccgatt ggcaaggctt
     7861 tggtaaacag cgtcagttag ctcaacaata tgtgaccagt gattatgtac tttggcttga
     7921 tgcagatgag cgagtgacag ccgaactgaa gcaatctatt ttacaagcgg tcaaaaatga
     7981 tgaaaaaaat accgtttaca aaatcagccg cttaagtgaa gtatttggca aacaaattcg
     8041 ccactcgggt tggtatccgg attgtgtggt gagattgtat cgcacggaat ttgccaaata
     8101 tggtgatgaa ttggttcacg aaaaagtaca ttacccaaaa gaggcaaaaa tccagaaact
     8161 acaaggcgat ttgctgcatt tcacctataa agatattcac cactatttgg taaaatcggc
     8221 aagttacgct aaagcgtggg caatccaacg ggcaaatgca ggcaaaacag ccagcttatt
     8281 tgatggcgtg actcacgcca tcggctgttt tgtgaaaatg tatctattaa aagccggttt
     8341 tttagatggc aagcaaggat ttttattggc agttctttcg gcacattcta ctttcgtaaa
     8401 atatgcggat ttgtggaatc gtactcggaa atgatgcttt tcagtactct ctccctctag
     8461 tgggagagag atagataaaa ttgcgtttag caattttatc agagagaggg agtattccac
     8521 attctccgaa taaacggtac tatcgttttt aatgcctcag aaatagaaat taaagttaaa
     8581 ttcatttgtg aggctttatc agacggtggg caaaatgcaa tgtgtttatt cgcatcatta
     8641 atcggtttcc aacggcgtgg aatggcagaa agtcggctcg ggtagaagcc aatagttgga
     8701 atatttaacg ccccactaat atgtaacgga ccggttgagc cggcaataaa caaatctgca
     8761 cacgcaagcg aatgtgtaaa atcttgtaac ccctcatttt tatcataaac caccacgttg
     8821 ttgtgattga tttgttgagc taactgctga gctttttcgc tttcgccttt ccctgcggtg
     8881 agtaccacat agcaatcaaa ttctgccaaa ataccttgaa ttaactctgc atactgttct
     8941 atagataaat tatttgccga accaccggtt gaactatgca caaataccca ctttttaacg
     9001 gtggtaatat tgagttgttc gcataatttt gctttttgtg cctggacggt ttgctgttca
     9061 acacttaagt atggcgtgtg aggttcaaca atcggtatat tgtgtttgct taaaaaagca
     9121 cgggctaaat ccaaattata ttcggattcc gcttttgctg attgagaacg gcgttgggtt
     9181 aaacggtggt tatataaaaa ttggaataat ttagttgctg gagcgaggcg gtatttaatc
     9241 ccactttgcc aggtgagttt ggcattatgc cagtctgaaa caaaacaaat aaccgcatca
     9301 aattgctcat ttttaaccgc ttgtaaaata cggttaaatt catttttatc ttttttattc
     9361 gccgtatcaa taataatctt atcaatagaa gggcaaagct cggcaagtgg agcagtataa
     9421 cttggcacaa gagcggtaat ttccacattc ggcattgatt gcttcaacat agcaaatgcc
     9481 ggaaagcaga ccataaagtc cccaatttta tcattacgga ttacgagtag ttttttcatt
     9541 ctgattttct ctattgcttt taggttttca gtaaattata agcggtcaaa tttcacaaaa
     9601 aatttgcaaa tcatcgggct aaattttaga tgaattttta ctacttttca gctagaatat
     9661 tttgcttgaa cgtaagcgtt tgcgtttgaa tatttttact ttcaaaattt ataggaattc
     9721 gtatgtcagc aacaatttta gaagctttac ctttagggca gaaagtgggt atcgcctttt
     9781 caggcggttt agatacctca gcagcactgt tatggatgcg taaaaaaggg gcagtgcctt
     9841 atgcgtacac cgcaaattta ggtcagccgg atgaagatga ttacaacgca attccgaaaa
     9901 aagcgatgga atatggggct gaaaatgcac gattaatcga ctgtcgccaa cagttagctc
     9961 acgaaggcat tgccgcaatt caatgcggtg ctttccatat ttcaaccggc ggtattccgt
    10021 atttcaacac cacgccactt ggtcgtgcgg taaccggcac aatgttggtt gcggcaatga
    10081 aagaagatga tgtgaatatc tggggcgatg gttcaacctt taaaggtaac gatattgagc
    10141 gtttctatcg ttacggttta ttaaccaatc caaaattaaa aatttataag ccgtggttag
    10201 accaactatt cattgatgaa ttaggcggtc gcttggaaat gtctaaattc ttaatcgaaa
    10261 acggttttga ctataaaatg tcggtggaaa aagcctactc tacagactcg aatatgttag
    10321 gtgcaaccca cgaagcgaaa gatttagaag aattaagcac cggtatgaag atcgtaaaac
    10381 cgattatggg cgtggcattc tgggacgaat cagttgaaat taaagcggaa actgtttctg
    10441 ttacctttga agaaggtgtg ccggtggcgt taaacggtaa acgtattgat gatgccgttg
    10501 aattaattat ggaggctaac cgcattggtg gcagacatgg tttgggaatg tcagaccaaa
    10561 tcgaaaaccg tattatcgaa gcgaaatctc gtggtattta tgaagcaccg ggaatggcat
    10621 tattacacat cgcttacgag cgtttagtta ccggtattca caacgaagat acgattgagc
    10681 aatatcgcat taacggatta cgtttagggc gtttattgta tcaaggccgc tggtttgacc
    10741 cgcaagcttt aatgttgcgt gaaacggctc aacgttgggt ggcaaaagcg attaccggta
    10801 cggtcacgct tgagttacgc cgtggtaacg atttcactat tcttaacact gaatcgccaa
    10861 acttaaccta cgaagcagaa cgtttaagta tggaaaaagt agaagatgca ccgtttacgc
    10921 cggcagatcg tatcggtcaa ttaacaatgc gtaacttaga tattgtcgat acccgtggta
    10981 aattagcagt ttataccgat gcaggtttat tggcggctaa taatagcgtg attccacaac
    11041 ttggaaaaaa ataatcagtt gatctccatt ctgataactt ttagaattta gcctaaaagc
    11101 tgccccaaag ttaatgcttt ggggctttta ttttcaaagc gtgatgatga aattagttga
    11161 aatttttaca gacggttctt gtttaggcaa ccccggcaaa ggggggattg gtattttgct
    11221 gcgttataac gggtacgaaa aaaccgtgag caaaggctat tttcaaacca ccaataaccg
    11281 aatggaattg cgggcggtga ttgaggcttt agcaatgcta aaagagcctt gtaaggtgca
    11341 actcaatagt gacagccaat atatgaaaaa cgggatccaa aaatggattt ttaactggaa
    11401 gaaaaatgac tggaaaacca gcgataaaaa gccagttaaa aataaagatt tatgggttgc
    11461 tttagaccaa gaaattcaac gccaccaaat tgaatggagt tgggtgaaag ggcattcagg
    11521 acatcgtgaa aatgagattt gcgatgagct ggctaaacaa ggagcaaata atccaacgtt
    11581 ggacgatgtt ggttatattg cagactaggt tttacaagcg gtcggattgt gtaaattttt
    11641 taccgaattt aaccgcttgt atttgcataa agcacttatt tttagcgaaa tagtgctacg
    11701 caaacgtttt ctttttttgt agaataggag agctttacgc tttaaggtgt gttatggttg
    11761 atttaattgt tatcggaatc attgcttttt cagtgcttat cagtttatgg cgaggtttta
    11821 ccaatgaagc attatcgcta cttggttggg taatcgcctt ttttgtggca agctcgtttt
    11881 atccttattt aagcggttat ctcacacaaa ttaattcggt ttatttgcaa aattcagaat
    11941 ttttacgcaa tggtattgca gcggcgattc tgttcatttt gactttaatt gtttgtggtg
    12001 tgatcaacgg tttattaggc aaagtgattg atttgagcgg aatgtctatc accgaccgta
    12061 ttttaggcgg tgcttttggg gcattgcgtg gtattttaat tgtgtcggct attttatttt
    12121 tcttagatac ctttacccaa gcaagccaaa cggaattatg gaaatcatcg ctattaattc
    12181 cgcattttga ctttattgtg aagtggttct ttgaacagtt acaagcaaat tccagttttt
    12241 taaacacgac taaataaaga ggtttttcta tatgtgcggc attgtcggta ttattgggaa
    12301 ttcgccggtg aatcaggcga tttatgatgg tttaacatta cttcaacacc gaggacaaga
    12361 tgccgcaggt atcgtcacca tagacgatga aaatcgtttc cgcttacgca aagctaacgg
    12421 cttagtcagc gatgttttcc agcaagagca tatggtgaga ttacaaggca atgttggaat
    12481 tggtcacgtt cgctacccaa cagcaggtag ctcaagtgtg tctgaagccc agccatttta
    12541 tgtcaattca cctttcggta ttaccttagt tcacaacggt aatttaacta ataatgcgga
    12601 acttaaagct cgcttataca acgaagcccg ccgccatgtg aacactaatt ctgattctga
    12661 atcccttctt aatatttttg cttacttttt agatctctat tccactcagc atttaagccc
    12721 agacaatatc tttgaaacgg ttcgtaaaac caatgatagc attcgtggtg cttatgcttg
    12781 cattgcgatg attatcggac acggtatggt tgctttccgt gacccattcg gtattcgccc
    12841 gttagtgctg ggtaaacgtg aaatcgaggg taaaaccgaa tatatgtttg cttcggaaag
    12901 tgtggctctt gatgtagtgg ggtttgaatt tgtgcgagat gtgctgccgg gtgaagcgat
    12961 ttatgttacc tttgatgggc aattacattc gcaaatttgt gccgataatc caaaactgaa
    13021 tccttgtatt tttgaatatg tttattttgc ccgtcctgat tccgtcattg atggcgtttc
    13081 tgtatattct gcacgagtgc atatgggcga attattaggt gagaaaatta aacgtgaatg
    13141 gggacgaatt atcgatgata ttgatgtggt gatcccgatt cctgaaacct caaatgatat
    13201 tgcggtacgt attgctaata tgttgtataa accctatcgt caagggtttg ttaaaaaccg
    13261 ctatgtagct cgaactttta ttatgccggg gcaagcacag cgtaaaagct cggttcgccg
    13321 taaattaaat gcgattgcct ctgaatttaa aggcaaaagc gtgttactgg ttgatgattc
    13381 tattgtacga ggtacaacgt ctgaacaaat cgtggaaatg gcacgagcag ctggtgcaaa
    13441 acgggtttat tttgcctctg ccgcaccgga aattcgctac ccgaatgtgt atggcattga
    13501 tatgccgact tgtgaagaat tagtggctta tgatcgctca gtggaagagg ttgcacagat
    13561 gataggggtg gataaattga ttttccaaga ccttgaagca ctttataagt ctattcaact
    13621 ggaaaatccg actattcatc gctttgatga ctctgtattt acaggagaat atattacagg
    13681 tgatgtagat aaatgctatt tagacagtat agcaagatct cgaaacgata aagcaaaagc
    13741 agaggcggca aaacaagcca ccaatttaga aattcataac gaaagataaa agaaactcgc
    13801 agtgaaaact gcgagttttt tactattgat ttttcttag
//
LOCUS       DANLXF010000050        13798 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.050, whole genome shotgun sequence.
ACCESSION   DANLXF010000050 DANLXF010000000
VERSION     DANLXF010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13798
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.050"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            98..1075
                     /locus_tag="PXD66_000911"
     CDS             98..1075
                     /locus_tag="PXD66_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HDL6319233.1"
                     /translation="MSRNLFQKRLNIKPYEYPELLEFKDAIRHSYWLHTEFNFTGDIQ
                     DYRTTINDHERHVLTRAMLAISQVEVNVKRFWGELYCYFPKPEMDDVGGTFAESEVRH
                     KDAYSFLLEKLGLNEMFSQIHEIEPLMNRIRYMESFMKDKDAGKGEFVLSLVLFSLFV
                     EHISLFGQFIIMMSFNKHKNLFKGISNAVEATSKEEEIHGKFGIALYEILRDEHSELF
                     TPEFFEDLQTLANQAFEAERGILDWIFEEGDLSFITKATVENYIKNRYNNSLSILGLE
                     PPHDIDPELLKETEWFDIEILSTKETDFFNKRSTDYSKKMKQITADDLF"
     gene            1062..2729
                     /locus_tag="PXD66_000912"
     CDS             1062..2729
                     /locus_tag="PXD66_000912"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HDL6319234.1"
                     /translation="MTYFNTDRPDFHWLNEDSRLFLQRGYLLEGTTALDRIRFIAEHA
                     ERKLGIEGYADKFYHYMARGYFSLSSPIWSNFGLDRGLPISCFGSYIGDSIHEIMVTT
                     AEVGMMSKIGGGTSAYFGDIRPRGSLIKNNGKSDGSFNFSKLFDTVIDVISQGTSRKG
                     QFAGYIDIEHGDIDEWLDIHTEGNPIQLMYYGVCVGHDWLESMKAGDPYKRQLWAKLL
                     QRKTETGIPYLFFKDNANAGRPDVYKDKNMTIHASNLCTEIMLPSSNEESFVCCLSSM
                     NLLYFDEWKDTDAPETLTYFLDVVMSEFIEKSQSIPFLDRANRFARRHRALGLGVLGW
                     HSYLQANNIAFDSFQAMQKNNEIFKTLQAKTLKASQELAQRFGEPEILEGYGRRNTTL
                     MSIAPTKSSSFILGSVSPSVEPFKSNYYVKDLAKIKTVYKNPFLEKLLQEKGLDTEEI
                     WTSILQNDGSVQHLEQLSEHEKEVFKTFAEISQLSVIQQAAQRQKYIDQGQSINVMIH
                     PATPARDLNQLYLTAEELGLKSIYYQNSMSAAQVFNRNLLSCSSCEG"
     gene            2733..3152
                     /locus_tag="PXD66_000913"
     CDS             2733..3152
                     /locus_tag="PXD66_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319235.1"
                     /translation="MGNLSHTSPNTIVEVVGEFPEITSSGYTFDKGPIQLRIGKHTAP
                     NKGFGVKHIEAEHMQEILNLGFTSVNEFVKAIIVENAKIFCEFSDLKGNHRPIILKAS
                     IGIVVLERKFLESGTIYSVVTAYRRTNPHGIQIGTMK"
     gene            complement(3387..4148)
                     /gene="nudC"
                     /locus_tag="PXD66_000914"
     CDS             complement(3387..4148)
                     /gene="nudC"
                     /locus_tag="PXD66_000914"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HDL6319236.1"
                     /translation="MNKGYWIIVSDFDIALEAGEIPFGSAEELGLSGREKLTIGQYQG
                     SPAYLVKALESDNQREFAYLRSQINRPPELSHLLHRAVSLNHFFQTHQFCGKCGEKTI
                     LASDEIAIYCNACGNRAYPTISPSIIVAVRKGRQILLANHLRHKGTIYTTLAGFVEAG
                     EAVETAVHREVFEETGIKVKNVRYFGSQPWAFPNSLMLGFLADYESGEIQLQEEEICD
                     AKWFDCEESLPEIPQQGTIAFELIQATLKLCRESK"
     gene            complement(4141..4824)
                     /locus_tag="PXD66_000915"
     CDS             complement(4141..4824)
                     /locus_tag="PXD66_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HDL6319237.1"
                     /translation="MKQPTFKRMTNKRSNFAKNLQKNPQKRPLTFAETTLVLLNKPYD
                     VLTQFTDEQGRKTLKDFITMPNIYPVGRLDRDSEGLLLLTNNGEIQHRLAHPKFDKQK
                     TYWVQVEGVPQPEDLAKLAKGVELKDGLTKPAKVRAIATPDFAWNIAPQIRERKTIPT
                     SWIELIISEGKNRQVRRMTAHIGFPTLRLIRVGIGQFRLGNLGCGEFQVLSENEKRKL
                     FTQLGLKDE"
     gene            complement(4821..5669)
                     /locus_tag="PXD66_000916"
     CDS             complement(4821..5669)
                     /locus_tag="PXD66_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL6319238.1"
                     /translation="MEKRTLALLKVHGTAVLFGASGIFGALIQSSAETIVLGRVMIAF
                     AIISLYFLWKKQPLVKLNLQQMAQLAFSAALLAAHWVTFFVAVKVGGVAVATLGFASF
                     PAFVALFESLFFREKLRFREVVLLIAITVGLILVTPEFTFGNQATQGLLWGVLSALVY
                     GILAVANRRTMSKISGVQASWWQYIFATLFLLPLSITEFPSVSTQDWFWIFCIGFLCT
                     TLAYTLFISSLDTINARTASMIISLEPVYAIVIAWLCFNEVPTLKMIIGGLIILLSVA
                     WANLKK"
     gene            complement(5669..6154)
                     /locus_tag="PXD66_000917"
     CDS             complement(5669..6154)
                     /locus_tag="PXD66_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HDL6319239.1"
                     /translation="MPILAILFGIFFYIYCEISLLVNIGSIIGVLPTILLLVVISFLG
                     LWIVKLRGVYTLFSIKQDLSQGKIPTEAVGNSVMFIVAGILLIIPGFLSDILAILCVL
                     PFSRKWIQAFVINSVKNKVFMHFTAQTSSFSVTSAQDSNTFDAEFERKPDEQDQHNRI
                     K"
     gene            complement(6156..7478)
                     /gene="pepQ"
                     /locus_tag="PXD66_000918"
     CDS             complement(6156..7478)
                     /gene="pepQ"
                     /locus_tag="PXD66_000918"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HDL6319240.1"
                     /translation="MKNLFSTHLARLQQTVQSLLENAFLDGLWIYAGKAKTLFLDDQT
                     APFKINPYFNYFFPYPQAENCWLFLDGKNKPKIYFYAPEDYWVSTLKVPTEAFFAAEF
                     EWVLLRAENEIATFIQNPTACAFIGEDVELAKKLGFSHINPQKVLNSLNFARSIKTEF
                     EIESIYQAQFSALKGHQVAKEAFFAGKSEFEINLEYLKATQQSDLNVPYGNIVAINQN
                     GAVLHYTALDFDTHAKRHSFLIDAGTTVNGYASDLTRTYAFDSGSEFAELVAKMEQLK
                     LQIIAELSIGVNYLHYHTRMQQMISQLLAEHQFIHLSAEQIFEQGISRTFFPHGLGHL
                     LGIQVHDVGGFQQNARGSHKAPPEVYPSLRCTRELQENMVLTIEPGFYFIEMLLKPWK
                     NSALSSAFNWQKIEAFKAFGGIRTEDNIVMRANGAENLTANAAATLGG"
     gene            7489..7731
                     /gene="tusA"
                     /locus_tag="PXD66_000919"
     CDS             7489..7731
                     /gene="tusA"
                     /locus_tag="PXD66_000919"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005710491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HDL6319241.1"
                     /translation="MTQLTVNKELDTLGLRCPEPVMLTRKTIRNMEEGEILHIIADDP
                     ATTRDIPSFCEFMDHQLIQKEVDSTPYHYWIKKGLE"
     gene            complement(7798..8229)
                     /gene="rraB"
                     /locus_tag="PXD66_000920"
     CDS             complement(7798..8229)
                     /gene="rraB"
                     /locus_tag="PXD66_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HDL6319242.1"
                     /translation="MYNLDELRTETDETISSLLADGSDPEALYIIEHHVAHHDFDKLE
                     KLVVDAYKLGYEISEAEEVEDENGKVIFVCDIVSEIHLNADLITAQQKELLPLIEKAG
                     AEYEGWGTYFEDPNADEDEYGDDGEFFDDENDDFEDEPRRH"
     gene            complement(8244..8855)
                     /locus_tag="PXD66_000921"
     CDS             complement(8244..8855)
                     /locus_tag="PXD66_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDL6319243.1"
                     /translation="MKLNYHKTHFLTSAPDIRHLPEDTGVEIAFAGRSNAGKSTALNA
                     LTNQKSLARTSKTPGRTQLINLFKVETNCKLVDLPGYGYAAVPEQMKIQWQKALGEYL
                     QKRECLGGVVILMDIRHPLKDLDQQMIEWAVSANLPVLLLLTKADKLSQSARSKQVKM
                     VREAILPFQGDIQVEAYSALNKIGIDKLSNKLDSWFAPKLITE"
     gene            complement(8857..9273)
                     /locus_tag="PXD66_000922"
     CDS             complement(8857..9273)
                     /locus_tag="PXD66_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDL6319244.1"
                     /translation="MSWYKALFSFNGRLNRRGFWIGFGIHFLFLFMVAMWINLAELSL
                     LSVLPLIITGFSLSAIIIKRLHDRNRSAKALLMALVPIICYLAAPYSQGIMQILLGQV
                     MPAFIGMLLFLEWGVFAGNPEPNQYGEQGLSFKFRE"
     gene            complement(9277..10107)
                     /locus_tag="PXD66_000923"
     CDS             complement(9277..10107)
                     /locus_tag="PXD66_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HDL6319245.1"
                     /translation="MFEYFLSLSPIWQAFLAGLFTWGCTIFGSSFVYFFKSVNRKLLD
                     SMMGFAAGVMIAASFWSLLAPSLEYAEADYGSLAWLPAAIGFLAGGAFIRLIDYVVPH
                     LHLSKPIEQAEGNDQLKQGLSKSTLLFLAITIHNIPEGLAIGVAFGALATQVSGVDAS
                     IMGAIGLAIGIGLQNIPEGSSLVLPIRGEGHSRKKAFWYGSMSAVVEPIAAVIGAVFV
                     LSMTAILPYALAFAAGAMIFVVVEELIPESQSSGNTDIATLSLMLGFVVMMVLDVALG
                     "
     gene            complement(10444..10559)
                     /gene="rrf"
                     /locus_tag="PXD66_000924"
     rRNA            complement(10444..10559)
                     /gene="rrf"
                     /locus_tag="PXD66_000924"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(10721..13619)
                     /locus_tag="PXD66_000925"
     rRNA            complement(10721..13619)
                     /locus_tag="PXD66_000925"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         4004 a         3225 c         2632 g         3937 t
ORIGIN      
        1 ataaaaaaga gtgtttgtca agacttgtct tgaaactttt tttgcataca atcatctacc
       61 agttccaaac ataaacttta agatagagcc tacgaatatg tctagaaacc tcttccaaaa
      121 acgcttaaat attaagccgt atgagtatcc tgagcttctt gaatttaaag atgcaattcg
      181 ccattcttac tggttacaca ccgaatttaa cttcaccggc gatattcaag actatcgcac
      241 caccattaac gatcacgaac gtcacgtgtt aacccgagca atgcttgcga tttcacaagt
      301 agaagtgaat gtaaaacgtt tctggggcga gctatactgc tatttcccaa aaccggagat
      361 ggacgatgta ggcggtactt ttgctgaaag tgaagttcgc cataaagatg cttactcgtt
      421 cctattggaa aaattaggcc taaatgaaat gttcagtcaa attcatgaaa ttgaaccatt
      481 aatgaaccgc attcgttata tggaaagttt tatgaaagat aaagatgctg gcaaaggcga
      541 atttgtttta tcgttagtgc tgttctcttt atttgttgag catatttcat tgtttggaca
      601 gtttattatc atgatgtcgt tcaacaagca taaaaacctg tttaaaggga tttccaatgc
      661 ggttgaagcc acctcaaaag aggaagaaat tcacggtaaa ttcggaattg cgttatacga
      721 aattttacgt gacgaacaca gtgaactatt cacaccagaa ttttttgaag atttgcaaac
      781 cttggcaaat caagcgtttg aagcagaacg tggcatctta gactggattt tcgaagaagg
      841 tgatttaagc tttatcacca aagcgacggt agaaaattac attaaaaacc gttacaacaa
      901 ttctttaagc attttagggt tagagccgcc acacgacatc gaccctgaat tattaaaaga
      961 gactgagtgg tttgatattg aaattctttc aaccaaagaa accgatttct ttaacaaacg
     1021 tagcaccgat tattctaaaa aaatgaaaca gattaccgca gatgacttat tttaatacag
     1081 accgcccgga cttccactgg cttaatgaag acagccgttt attcttacaa cgtggctatt
     1141 tattggaagg aacaaccgca ttagaccgca ttcgttttat tgccgaacac gcagagcgaa
     1201 aactcggcat tgaaggctat gccgataaat tctatcacta tatggcaaga ggctacttct
     1261 cgctttcttc gccaatttgg tcaaactttg gcttagatcg tggcttaccg atttcttgtt
     1321 tcggtagcta catcggcgac tctatccatg aaattatggt gactaccgct gaagtgggca
     1381 tgatgagtaa aattggcggt ggtacttcgg cctactttgg cgatattcgt ccgcgtggct
     1441 cgttaattaa aaacaacggt aaatcagacg gctcatttaa cttcagtaaa ttatttgaca
     1501 ctgtgattga cgtgatttca caaggaacat cacgcaaagg tcagtttgcc ggttatattg
     1561 atattgagca cggcgatatt gacgaatggc tggatattca caccgaaggc aacccaattc
     1621 aattaatgta ttacggtgtg tgtgtcggtc acgattggtt agagtcaatg aaagccggcg
     1681 atccatacaa acgtcagctt tgggcaaaat tgttacaacg taaaaccgaa accggtatcc
     1741 cttatttatt ctttaaagac aatgccaacg caggtcgtcc tgatgtatat aaagataaaa
     1801 atatgacgat tcacgcctct aacttatgta ctgaaattat gttgccttcc agcaacgaag
     1861 aaagtttcgt atgctgttta tcgtctatga acctactcta ctttgatgag tggaaagata
     1921 ccgatgcacc agaaacatta acctatttcc tcgatgttgt aatgagtgaa tttattgaga
     1981 aaagtcaatc aattccattc ttagaccgtg caaaccgctt tgcccgtcgc catcgtgcat
     2041 taggtttagg cgtgttgggc tggcacagct atctacaggc aaataatatt gcctttgaca
     2101 gcttccaagc gatgcaaaaa aacaacgaga ttttcaaaac gcttcaagca aaaaccttga
     2161 aagcctcgca agaattggct caacgcttcg gcgagccgga aattttagag ggctatggtc
     2221 gccgtaatac tacattaatg agtattgctc cgactaaatc cagtagcttt attttaggca
     2281 gtgtgtcgcc atcggtagag ccgtttaaat ccaactacta tgtaaaagat ttagcgaaga
     2341 tcaaaaccgt gtataaaaac ccgttcttag agaaattact ccaagaaaaa gggctagaca
     2401 ccgaagaaat ttggacgtct attttacaaa atgatggctc ggttcagcat ttggaacagc
     2461 tttccgagca cgaaaaagaa gtatttaaaa cctttgccga aatcagccaa ttaagcgtga
     2521 ttcagcaagc tgctcaacgt caaaaatata ttgaccaagg gcaaagtatt aatgtgatga
     2581 tccacccagc cacaccggct cgtgatctta accaattata cttaacagca gaagagctgg
     2641 gcttaaaatc catctactac caaaactcaa tgagtgcagc acaggtattt aaccgtaacc
     2701 tattaagctg ctcaagttgc gaagggtaaa taatgggaaa tttatcgcat acttcaccta
     2761 atactattgt tgaagttgtt ggtgaatttc ctgaaataac atctagtggg tatacctttg
     2821 ataaaggtcc tattcaatta agaatcggca aacatactgc tccaaataaa ggctttggtg
     2881 taaagcatat tgaagccgag catatgcaag aaattcttaa tctaggattt acatcagtta
     2941 atgaatttgt caaagctatc atagtagaaa acgctaaaat tttttgcgaa ttctcagatt
     3001 taaaaggcaa tcatcgccct attattctga aagcaagtat tggcattgtg gttctagagc
     3061 gtaagttttt ggaaagcggt acgatatatt cagttgtaac cgcttataga agaacaaatc
     3121 ctcacggaat acaaataggc actatgaaat aaagtacgtt agtgttataa tcagttccac
     3181 gcaagagcat ttgtagaaaa taacaggcgg atttggtttt ctactgtggg cggcaggttt
     3241 ccgctaacct gtaatagcta tttaattttt atattaaatc ctgaacttga aacagactaa
     3301 gggattgctg cggtagctaa gaatacaaac ctctctataa tcatatagag aggttttttc
     3361 ttattgatga gcacaagtta agataattat ttactctccc gacataactt caaggtagct
     3421 tgaatcagct caaaagctat tgtgccttgt tgtgggattt caggtaagct ttcctcacag
     3481 tcgaaccatt ttgcatcgca gatttcttct tcctgtagct gaatttcacc gctttcataa
     3541 tccgctaaaa aacctagcat taatgaattg ggaaacgccc aaggttggct tccaaaataa
     3601 cgcacatttt tcactttaat gcccgtttct tcaaacactt cacgatgcac cgcagtttct
     3661 accgcctcac cggcttcgac aaaaccggct aaagtggtat aaattgtgcc tttatggcgt
     3721 agatgattgg ctagtaggat ttgtctgcct tttcgcaccg ccacaataat tgacgggcta
     3781 atcgttgggt aagctcgatt gccacacgca ttgcagtaaa ttgcaatttc atcgcttgcc
     3841 agtatggttt tctcgccaca ttttccgcaa aattggtgag tctgaaagaa atggttaagc
     3901 gaaacagctc gatgcaacaa atgagacagt tcgggtggtc ggttaatttg gcttcttaaa
     3961 taagcaaact ctcgctgatt atccgattct aacgccttca ccaaataagc cggcgagcct
     4021 tgatattgcc cgatggttaa tttctcacgt ccacttaagc ccagctcttc cgcacttccg
     4081 aatggaattt ccccagcctc caaggcaata tcaaaatcgc tgacgataat ccaatatcct
     4141 ttattcatct ttcaatccta attgtgtaaa caacttacgt ttttcatttt cagatagcac
     4201 ttgaaactca ccacagccta aattgcctaa tctaaactgg cctatcccaa cacgaattaa
     4261 ccgtaaagtc gggaaaccga tatgtgcagt cattcttctg acttggcgat ttttcccttc
     4321 agaaataatc aattctatcc agctggtcgg aatggttttc cgctcacgaa tttgcggggc
     4381 gatattccaa gcaaaatcgg gcgttgctat tgctctgact ttcgcgggct tggtcaaacc
     4441 atcttttaac tccacccctt ttgctaattt ggcaagatct tccggttgtg gaacaccctc
     4501 cacttgtacc caataggttt tctgcttgtc aaatttcggg tgagctaaac ggtgctggat
     4561 ttcgccatta ttagtcaaaa gcaacaatcc ttcactgtct ctatctagcc gcccgaccgg
     4621 gtaaatattg ggcatcgtaa taaaatcttt tagcgttttt cgcccctgtt catcggtaaa
     4681 ttgggttagt acatcataag gcttattgag taacacaagg gtggtttccg caaaggtaag
     4741 cggtcgtttt tgcggatttt tttgcaaatt tttagcgaaa tttgaccgct tgtttgtcat
     4801 tcttttaaag gttggttgtt tcattttttc aggttcgccc acgcaacaga aagtaaaata
     4861 attaagccac cgataatcat tttaagggtt ggcacttcat taaagcaaag ccacgcgatc
     4921 acaatcgcat acaccggctc gagggaaata atcattgagg cagtacgggc gttgatggta
     4981 tcaaggcttg aaataaacag cgtataggca agggttgtac ataaaaaacc gatacagaaa
     5041 atccaaaacc aatcttgggt ggaaacgctt ggaaattcag tgatggagag aggcaacaga
     5101 aaaagtgtag caaaaatata ctgccaccag cttgcttgca caccggaaat tttgctcatg
     5161 gtacggcgat tcgctaccgc caaaatacca tacaccaaag cagataacac gccccacaat
     5221 aaaccttgag tggcttgatt accaaaagta aattcagggg taactaaaat taaaccaacc
     5281 gtgatggcaa ttaatagcac cacttcacga aagcgtaatt tttcacggaa gaacaggctt
     5341 tcaaataacg ccacaaatgc cggaaagctg gcaaagccta aggttgctac cgccacacca
     5401 cctactttaa ccgccacaaa aaaagtaacc cagtgggcag ctaataaagc tgcagaaaat
     5461 gcaagttgag ccatttgctg taagttgagc tttacaagcg gttgtttttt ccataaaaaa
     5521 tacaaagaga tgatagcaaa agcaatcatc actcgcccaa gcacaatggt ttcagccgag
     5581 ctttgaatta atgctccgaa gatccccgag gcaccaaata aaaccgctgt gccgtgtact
     5641 ttaagtagtg caagtgttct tttctccact atttgatcct attatgttga tcttgctcgt
     5701 ccggcttgcg ttcaaattct gcatcaaacg tgttgctatc ttgcgcagat gtaacagaaa
     5761 aagacgaagt ttgggcggta aaatgcataa acaccttatt ctttacgcta ttaattacga
     5821 aggcttgaat ccattttcgg ctaaagggta acacgcataa aatcgctaaa atatcactta
     5881 aaaagccggg aataatcagc aaaatgccgg ctacaataaa catcaccgag ttgccgactg
     5941 cttcagtcgg tatcttgcct tggcttaaat cttgcttgat ggaaaagagg gtataaacgc
     6001 cccttaactt cacaatccat aaccctaaaa atgaaatcac gaccagtaac aaaatcgtgg
     6061 gtaacacgcc aatgatcgag ccaatattca ccagcagcga aatttcgcaa tagatataaa
     6121 agaaaatacc aaacagaatc gctaaaatag gcatattatc ctcccaacgt tgcggcagcg
     6181 tttgccgtca gattttctgc cccattggct cgcatgacaa tattgtcttc ggtacgaatc
     6241 ccgccaaagg ctttgaaagc ctcaattttc tgccagttaa aggcagaaga taaggcactg
     6301 tttttccaag gcttaagtag catctcaata aaataaaaac ccggctcaat ggtaagcacc
     6361 atattttcct gtagctcacg ggtacaacgc aaacttgggt aaacctctgg aggagcttta
     6421 tggctgcctc gggcattttg ctgaaaaccg cccacatcgt gcacttgaat acccaataaa
     6481 tgccctagtc cgtgtgggaa gaacgtacgg ctaatccctt gttcaaaaat ttgctcggct
     6541 gaaagatgaa taaattgatg ttccgctaat aattgcgaaa tcatctgctg cattcgggtg
     6601 tgataatgca ggtagttcac cccaatactc agctcagcaa taatctgcaa cttcaactgt
     6661 tccattttcg ccaccagctc ggcaaattca gaaccgctgt caaaggcata ggtgcgggtg
     6721 agatcggagg cataaccatt taccgttgtg ccggcatcaa ttaaaaaact gtggcgtttt
     6781 gcgtgggtgt caaaatctaa cgccgtataa tgtagcaccg caccattttg attaatcgcc
     6841 acaatattgc cataaggaac attgagatca gattgctgcg tcgctttcaa atattcaagg
     6901 ttgatttcaa attcgctttt gccggcaaaa aatgcctctt ttgctacttg atgccctttt
     6961 aaggctgaaa attgggcttg gtaaatactt tcaatttcaa actccgtttt gatggagcgg
     7021 gcaaaattca ggctattcag cactttctgc ggattaatat ggctaaagcc taactttttg
     7081 gctagctcaa catcttcacc gataaaggca caagcggtcg gattttggat aaaggttgca
     7141 atttcattct ctgctctcaa caaaacccac tcaaattccg cagcgaaaaa ggcctccgtt
     7201 ggcactttta gagtggaaac ccaataatct tcaggggcat aaaaatagat tttcggctta
     7261 tttttgccat ctaaaaataa ccagcaattt tcagcttggg gatatgggaa aaagtaatta
     7321 aaataagggt taattttaaa cggggcggtt tgatcgtcta aaaataaggt ttttgccttg
     7381 ccggcataaa tccataaacc gtctaaaaag gcattttcca gcaagctttg cactgtctgt
     7441 tgcaaacgtg cgagatgcgt agagaataga tttttcataa ggaataaaat gactcaatta
     7501 actgtaaata aagaattaga taccttgggg ctacgttgcc cagagccggt gatgctcacc
     7561 cgtaaaacaa tccgtaatat ggaagaggga gaaattttgc atatcatcgc cgatgacccg
     7621 gccactaccc gtgatattcc gagtttctgc gaatttatgg atcaccaatt aattcaaaaa
     7681 gaggtggact ccaccccata ccattactgg attaaaaaag gcttggaata aaccacaagc
     7741 ggtcgatttt agccataaaa ttgcaaattt atgcacaaaa tcgaccgctt gttttaatta
     7801 atgacggcga ggttcgtcct caaaatcgtc attttcatca tcgaaaaatt caccgtcatc
     7861 gccgtactca tcttcatcag cattcggatc ttcaaagtag gttccccagc cctcatactc
     7921 cgcaccggct ttttcaatta atggcagtaa ctctttttgt tgtgcggtaa tgagatccgc
     7981 attcaagtga atttcgctca caatgtcgca tacaaaaatc actttaccat tttcgtcttc
     8041 cacttcttcc gcttcggaaa tttcatagcc cagcttatag gcatcaacta ctaatttttc
     8101 caatttatca aaatcgtggt gggcaacgtg gtgttcaata atataaaggg cttcagggtc
     8161 tgaaccatcg gctaataatg atgaaatggt ttcatcggtt tctgtgcgta attcatcgag
     8221 attgtacata ttgaattcct tacttattct gtgattaatt ttggtgcaaa ccagctatct
     8281 aatttgttcg acagcttatc aataccgatt ttattcagtg cggaataagc ctctacttgg
     8341 atatcacctt ggaatggtaa aatcgcttct cttaccattt ttacctgctt agagcgtgcc
     8401 gattggctta acttatccgc tttggtcaac agcagtaaca ccggcaagtt ggctgacact
     8461 gcccattcaa tcatctgttg atctaaatct ttcaacgggt ggcgaatatc cattaaaatc
     8521 accacaccgc ctaaacattc acgcttttgt aaatactcgc ctaatgcttt ttgccactgg
     8581 attttcatct gttcaggtac tgctgcatag ccatagccag gtaaatccac taatttacaa
     8641 ttcgtttcaa ccttaaacaa gttaatcaac tgcgttcgcc ccggtgtttt ggaagttcgg
     8701 gctaaacttt tttggttagt taaagcgttc aaagcagtcg atttaccggc atttgaacgc
     8761 ccggcaaaag caatctccac acctgtatct tcaggtaaat gacgaatatc cggtgctgaa
     8821 gttaaaaaat gggttttatg atagtttaat ttcattttat tctctaaatt taaaagataa
     8881 gccttgctcg ccatattgat tcggttcagg attaccggca aatacacccc attctaaaaa
     8941 gagcaacatt ccaataaatg ccggcatcac ctgtccaagc aatatctgca taattccttg
     9001 tgaataagga gctgcaagat aacaaataat cggcactaat gccattaaca gggcttttgc
     9061 cgaacgattt cggtcgtgta atcgtttaat aataatagca gacaggctaa atcctgtaat
     9121 tataagcggt agcacactca ataaagacaa ttcagccaaa tttatccaca tagcaaccat
     9181 aaacaggaaa agaaagtgga ttccaaatcc aatccaaaaa cctcgtctat ttagcctacc
     9241 gttaaaacta aaaagtgctt tataccaact cattttctac cctaaagcta catcaagtac
     9301 catcattact acaaagccta acatcaggct taaagtcgca atatcagtat ttcccgaact
     9361 ttgcgattcc ggaatcagtt cttctacgac cacaaaaatc atcgctccgg cagcaaaggc
     9421 aagagcataa ggtaaaatag ccgtcatcga cagcacaaaa accgccccaa tcaccgccgc
     9481 aataggctca accactgctg acatagagcc ataccaaaat gctttcttcc gactatgccc
     9541 ttcgcctcga attggcaata caagtgacga gccttcggga atgttttgta accctattcc
     9601 gattgccaaa ccgatagctc ccataatgga agcatctacg cctgaaactt gggttgccaa
     9661 agcaccgaaa gccaccccta tggctaatcc ttctggaata ttatgaatgg taatcgcaag
     9721 aaaaagcaaa gtagattttg ataagccttg tttaagttga tcatttcctt cagcttgctc
     9781 aatcggctta ctcaagtgca aatgcggcac aacatagtca attaatcgaa taaaagctcc
     9841 accggcaaga aagccaatcg ctgcaggcag ccaagctaaa gagccataat ccgcctcggc
     9901 atattctaaa gaaggtgcaa gcaaagacca aaacgaggct gcaatcatta cacctgcggc
     9961 aaagcccatc atactgtcta ataattttcg attaaccgat ttaaaaaaat agacaaatga
    10021 cgaaccgaaa atcgtacaac cccaagtaaa taaacccgct aaaaaagctt gccatattgg
    10081 agaaagtgaa aggaaatatt caaacaagaa atagtaccag ctaaaataaa tattggctca
    10141 ttctatcaaa gattcagggt tattaatata gtgtagccaa aggctcaacg aagatttcac
    10201 ttgtaaaact tagcaagaaa ataaccgctt gcatttccct ttcataaata caatgaaaat
    10261 cctaacaagc ggtcattttc cgtgagtttt ttacctttac cacaacaaca aaaaaccgcc
    10321 tgtatctcta caggcggctc tttcttggat tacttgtact ttgtttattt gtacttattt
    10381 tgcttttgct taagttttcg cttttgtaac ttttagcttt cgcttaagct cgcttaaatt
    10441 aaaacccgac gatgctctac tctcacatgg cgaatcacca cactaccatc gacgttactg
    10501 cgttttactt ctgagttcgg tatggggtca ggtaggacca cagcactctt gtcgtcggga
    10561 aaattctttt ctatttttga atttctttaa gttaacttcc taatctaact cttagttcac
    10621 tttaatagtt agattaactt ccttaacttc gattgatttc ttctctagtt tcgttcttct
    10681 tctcaataca aaaacaagcc taaaaacatt tgagcgttgt atagttaagc ctctcgggca
    10741 attagtactg gttagctcaa tgtctcacaa cacttacaca cccagcctat ctacgtcgta
    10801 gtctccaaca acccttacac acttatagtg tgggagaact catctcgagg caagtttcgt
    10861 gcttagatgc tttcagcact tatctcttcc gcatgtagct acccagcaat gcctctggcg
    10921 agacaactgg aacaccagtg atgcgtccac tccggtcctc tcgtactagg agcagcccct
    10981 ctcaattctc caacgcccac ggcagatagg gaccgaactg tctcacgacg ttctaaaccc
    11041 agctcgcgta ccactttaaa tggcgaacag ccataccctt gggacctact tcagccccag
    11101 gatgtgatga gccgacatcg aggtgccaaa caccgccgtc gatatgaact cttgggcggt
    11161 atcagcctgt tatccccgga gtacctttta tccgttgagc gatggccctt ccattcagaa
    11221 ccaccggatc actatgacct gctttcgcac ctgctcgact tgtctgtctc gcagttaagc
    11281 ttgcttatac cattgcacta acctcacgat gtccgaccgt gattagcaaa ccttcgtgct
    11341 cctccgttac tctttgggag gagaccgccc cagtcaaact acccaccaga cactgtccga
    11401 gaccgcgttc cgcaatcttc gttagaacat caaacgttaa agggtggtat ttcaaggacg
    11461 cctccacaat cactggcgtg actgcttcaa aggctcccac ctatcctaca catcaaaatt
    11521 caatgttcag tgtcaagcta tagtaaaggt tcacggggtc tttccgtcta gccgcgggta
    11581 caccgcatct tcacggcgat ttcaatttca ctgagtctcg ggtggagaca gcctggccat
    11641 cattatgcca ttcgtgcagg tcggaactta cccgacaagg aatttcgcta ccttaggacc
    11701 gttatagtta cggccgccgt ttactggggc ttcgatcagg agcttctctt gcgataacac
    11761 catcaattaa ccttccagca ccgggcaggc atcacaccct atacgtccac tttcgtgttt
    11821 gcagagtgct gtgtttttaa taaacagttg cagccagctg gtatcttcga ccggttcaac
    11881 cttcatccgc aagggactac aatctacgcc ggcgcacctt ctcccgaagt tacggtgcta
    11941 ttttgcctag ttccttcacc cgagttctct caagcgcctg agtattctct acctgaccac
    12001 ctgtgtcggt ttatagtacg gtttataata acctgaagct tagtggcttt tcctggaagc
    12061 gtggtatcga ttacttcatc tccgtagaga ctcgtcatca cttctcggtg ttaaggaaga
    12121 ccggatttgc ctaatcttcc cacctaccgg cttaaacaga catccaacag tctgctaacc
    12181 taaccttctc cgtccccaca tcgcagttat tacaagtacg ggaatattaa cccgtttccc
    12241 atcgactacg cttttcagcc tcgccttagg ggccgactca ccctgccccg attaacgttg
    12301 gacaggaacc cttggtcttc cggcgaacga gtttttcact cgttttatcg ttacttatgt
    12361 cagcattcgc acttgtgata tctccagcag acttctcaat ccaccttcat cgacttacac
    12421 aacgctcccc tacccaacag actttcgtct gatgccgcag cttcggtgct atatttgagc
    12481 cccgttacat cttccgcgca ggccgactcg actagtgagc tattacgctt tctttaaatg
    12541 gtggctgctt ctaagccaac atcctagctg tctaagcctt cccacttcgt ttcccactta
    12601 atatagactt tgggacctta gctggcggtc tgggttgttt ccctctccac gatggacgtt
    12661 agcacccacc gtgtgtctcc tgagtatcac tcttcggtat tcgcagtttg catcgggttg
    12721 gtaatccggg atggacccct agccgaaaca gtgctctacc cccgaaggtg tccgctcaag
    12781 gctctaccta aatagatttc ggggagaacc agctatctcc cggtttgatt ggcctttcac
    12841 ccccagccac aagtcatccg ctaatttttc aacattagtc ggttcggtcc tccagttagt
    12901 gttacccaac cttcaacctg cccatggcta gatcaccggg tttcgggtct ataccttgca
    12961 actacacgcc cagttaagac tcggtttccc tacggctccc ttattcagtt aaccttgcta
    13021 caaaatataa gtcgctgacc cattatacaa aaggtacgca gtcacaagat tactcttgct
    13081 cccactgctt gtacgcacaa ggtttcaggt tctatttcac tcccctcgcc ggggttcttt
    13141 tcacctttcc ttcacagtac tggttcacta tcggtcaatc aggagtattt agccttggag
    13201 gatggtcccc ccatcttcaa acaggatatc acgtgtcccg ccctacttgt tgttagccta
    13261 gtaccacaat ggagttttaa ggtacgggat tatcaccctc tacgattgtc cttcccagaa
    13321 cattccccta actttactgc tatcactaac tggctctttc gcgttcgctc gccgctacta
    13381 acgaaatctc ggttgatttc ttttcctcgg ggtacttaga tgtttcagtt ctcccggttt
    13441 gcctcacaca gctatgtatt cactgggtga tagtaggttc ttcacctact gggtttcccc
    13501 attcggacat cttggattaa acgcctctta tcgactcatc caagcttttc gcagattagc
    13561 acgtccttct tcgcctctga ttgccaaggc atccaccttg tgcgcttagt cacttaacta
    13621 tacaacctca aatgttttct aaaacttctc aaaaataaaa gtaaaaaaac aatgaagtca
    13681 gttttacaac taaacacttg actgcttttg ttcagtcaag attttttcta ctcagacttt
    13741 caatttattc gttatcactt atgtataatt acttatgcgt aacgaacttg aaatatct
//
LOCUS       DANLXF010000051        13718 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.051, whole genome shotgun sequence.
ACCESSION   DANLXF010000051 DANLXF010000000
VERSION     DANLXF010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13718
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.051"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(30..746)
                     /gene="rph"
                     /locus_tag="PXD66_000926"
     CDS             complement(30..746)
                     /gene="rph"
                     /locus_tag="PXD66_000926"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDL6319246.1"
                     /translation="MRPNNRANNQVREIKITRNYTRYAEGSVLIEFGETKVLCNATVE
                     ESVPRFLKGQQQGWVTAEYGMLPRATHSRTQREAAKGKQGGRTMEIQRLIARSLRAVV
                     DLKALGERTVTVDCDVIQADGGTRTASITGACVALYDAMNKLVANGTLKTNPMKGLVA
                     AISVGIVAGEAVCDLEYLEDSNAETDMNVVMVEDGRLVEVQGTAEGEPFSHQELLTLL
                     DLAKQGISQLVEAQRKALAE"
     gene            1027..1890
                     /locus_tag="PXD66_000927"
     CDS             1027..1890
                     /locus_tag="PXD66_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011272075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDL6319247.1"
                     /translation="MIYSMTAFAHLEMKKEWGNAVWEIRSVNQRFLETYFRLPEQFRN
                     LEMTLRERLRSALTRGKVECSLRIDLSKTQNSEIALNKEYAAQVVQSLKWLKETANEG
                     EINLVDVLRFPGVVDNESQDLDQIAQDLLAGFEQILADFIAMRAREGENVQALILQRL
                     EAISVEAAKVQAQMPEILQWQKDRLQQRFDELNLQLDPQRLEQEMVLLAQRVDVAEEL
                     DRLQLHVKETTNILKKGGAVGRKLDFMMQELNRESNTLASKSINADVTNSAVELKVLI
                     EQMREQIQNLE"
     gene            1899..2789
                     /locus_tag="PXD66_000928"
     CDS             1899..2789
                     /locus_tag="PXD66_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HDL6319248.1"
                     /translation="MAMNFKQVSQSYPNLCVPLSQYCLIEIAGVDAEKYLQGQLTCDV
                     AKISVGEHTLTSHCDPKGKMSALLRLYRAENEKFMAIIHRSLLPEALTQLKKYAVFSK
                     VTFTELDTPLYGMAGEAAFAKLSENMTALRLTTGQPRAIVWGEELETTADEQLWTLMD
                     IQDGIPVLLQQNQFELIPQATNLQALESAISFTKGCYIGQETVARAKYRGANKRALFT
                     LAGNFESEISLPEVASAIEMQLGENWRATGTVLTSATDENTLWVQVVLNKDVEPNTKF
                     RANGVALEMVELPYSLAENE"
     gene            complement(2821..3081)
                     /locus_tag="PXD66_000929"
     CDS             complement(2821..3081)
                     /locus_tag="PXD66_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HDL6319249.1"
                     /translation="MALLITHKCTNCDMCLPECPNEAISVGDDIYIIDPVLCTECVGH
                     YEKPTCQKVCPITNCIIKDPDNIETEEQLWERFVMIHHPDQI"
     gene            3311..3730
                     /gene="rbsD"
                     /locus_tag="PXD66_000930"
     CDS             3311..3730
                     /gene="rbsD"
                     /locus_tag="PXD66_000930"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005753460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDL6319250.1"
                     /translation="MKKTLNLNAQLSHSIATLGHTDSLTVCDAGLPIPNTIERIDLAL
                     TAGVPSFLQTFDVVVSELFVERVLLAEEIKHKNPQVLAALLERLTQLEQTQQNKIQID
                     YVNHEIFKEQTHYSKAVVRSGECSPYANIILYSGVPF"
     gene            3740..5236
                     /gene="rbsA"
                     /locus_tag="PXD66_000931"
     CDS             3740..5236
                     /gene="rbsA"
                     /locus_tag="PXD66_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HDL6319251.1"
                     /translation="METLLKMSGIDKEFPGVKALSNACLSVYGGRVMALMGENGAGKS
                     TLMKVLTGIYSKDAGSISYLGKEVTFKGPKDSQEAGISIIHQELNLVGNLTIAENIFL
                     GRESKTAWGAIDWKKMYAEADKLLARLGVKHSSHQLCAELSIGEQQMVEIAKALSFES
                     QVIIMDEPTDALTDTETASLFKVIRELKAENRGIVYISHRLKEIFEICDDVTVLRDGQ
                     FIGERVIADIDEDQLIEMMVGRRLEEQYPHLHQERGELLLEVKNLSGSGVNNVSFQLH
                     KGEIVGISGLMGAGRTELMKVLYGALPKTAGSIELNGKAISNHCPQDGLNNGIVYISE
                     DRKGDGLILGMSVKENMSLTSLDHFSKAGSIRHDAEKLAVDDFIEMFNIKTPSREQQI
                     GLLSGGNQQKVAIAKGLMTRPNVLILDEPTRGVDVGAKKEIYQLINEFKKDGLSILMV
                     SSDMPEVLGMSDRILVMHEGQISAEFSRAEATQEKLLAAAIGKSTGLN"
     gene            5246..6214
                     /gene="rbsC"
                     /locus_tag="PXD66_000932"
     CDS             5246..6214
                     /gene="rbsC"
                     /locus_tag="PXD66_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005753457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HDL6319252.1"
                     /translation="MATQTKQFQLGRFLIEQRSFVALFILIVSVSMINPDFFSVDNIL
                     NILRQTSVNAIIAVGMTFVILIAGIDLSVGSVLALTGAVAATLVGSELPILLVIPLVL
                     LLGTAFGGISGAIVAKGKVQAFIATLVTMTLLRGITMVYTDGRPISTGFSDQADAFAF
                     IGTGYLFGIPVPIWIMAVVFGLAWYILKHTPIGRYIYALGGNESATQLSGINVNKIKI
                     FVFAVSGFLSALAGLIVTSRLSSAQPTAGVSYELDAIAAVVVGGTSLMGGKGRVMGTL
                     VGALIIGFLNNALNLLDISSYYQMIAKALVILAAVLADNYLGTKKV"
     gene            6237..7115
                     /gene="rbsB"
                     /locus_tag="PXD66_000933"
     CDS             6237..7115
                     /gene="rbsB"
                     /locus_tag="PXD66_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006995182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HDL6319253.1"
                     /translation="MKKLTTIASAVMLSLSVAATASAKETIALAVSTLDNPFFVSLKD
                     GAQKKADELGYKLVVLDSQNDPAKELSNVEDLTVRGAKVLLINPTDSEAVGNAVAIAN
                     RNKIPVITLDRGAAKGEVVSHIASDNVAGGKMAGDFIAQKLGSGAKVIQLEGIAGTSA
                     ARERGEGFKQAIEAHKFNVLASQPADFDRTKGLNVTENLLSSKGDVQAIFAQNDEMAL
                     GALRAVSSAKKEVVIVGFDGTDDGVKAVESGKLAATIAQQPELIGSLGVETADKVLKG
                     EKVEAKIPVDLKVISK"
     gene            7236..8165
                     /gene="rbsK"
                     /locus_tag="PXD66_000934"
     CDS             7236..8165
                     /gene="rbsK"
                     /locus_tag="PXD66_000934"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDL6319254.1"
                     /translation="MKKLTVLGSINADHVISVPYFAKPGETLTGHSCHIAYGGKGANQ
                     AVAAARLGAKVSFIGCIGSDDIGKAMKSAFEQDGIDTRSIKTIPNEMTGIAMIQVAES
                     GENSIVISAGANGHLDETVVAKCAAEITQADYLLMQLETPLPAIVQAAKLAQQNGTKV
                     ILNPAPARALPDELLNLLDMITPNETEAEILTGVKVVDEASAIQAAQVFHQKGIEKVL
                     ITLGSKGVFVSENGVGKIVAGFRVQAVDTTAAGDTFNGALVTAMLEDKPLDQAIRFAH
                     AAAAISVTRKGAQPSIPTRQETLDFLSQQAVSF"
     gene            8179..9189
                     /locus_tag="PXD66_000935"
     CDS             8179..9189
                     /locus_tag="PXD66_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL6319255.1"
                     /translation="MATMKDIARLAQVSTSTVSHVINNSRFVSEEIRERVQRVVKELN
                     YTPSALARSLKVNETKTIGMLVTATGNPFFAEVMAGVEQYCQQHQYNLIIATTNGDTE
                     RLQHHLQMLMQRKVDGLLLMCGDTRLNADESLNIPLPLVVLDWWFTELNADKIFENSI
                     SGGYLATKTLIEAGHSKIAIITGNLKKSLAKNRLEGYKKALAEANIPLNPNWVFESHF
                     DFAGGVNGMNALLAQSEKPTAVFACSDTIALGAYQAIWQQGLRIPQDISIIGYDDIEL
                     AKYLSPPLSTISQPKAELGRLAVETLLKRIRFKSTDFQTIMLEPELVLRHSIQTINKN
                     AE"
     gene            complement(9225..9854)
                     /locus_tag="PXD66_000936"
     CDS             complement(9225..9854)
                     /locus_tag="PXD66_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL6319256.1"
                     /translation="MTAPTQILLIDDHPLMRQGMRQLLELDHQFSVVGEASNGTDGIK
                     LALRLEPDVIILDLNMKGLSGLDTLRSLRAQGIDARIIVLTVSDEQSDVFALMDAGVD
                     GYLLKDTDTPELLDNIRKAAAGEVVLSETVRQHLLNRQPENDPLSCLTDRELDVLQWL
                     ATGMSNKQIAAQLFISEETVKVHIRNLLRKLNVHSRVAATVLYLEQQKG"
     gene            10052..12211
                     /gene="rlmKL"
                     /locus_tag="PXD66_000937"
     CDS             10052..12211
                     /gene="rlmKL"
                     /locus_tag="PXD66_000937"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HDL6319257.1"
                     /translation="MTNQTTYFATAARGFEEILKTELEQICAAECKVAQGGVHFTTTQ
                     KGAYQALLHSRLASRILLPLISTKIFSDSDLYASIVSFNWADLFDPRDTFYIDFSGTN
                     REIRNTQFGAMRVKDGIVDYFERKGFARPTVDKDNPDVRIHVYLDRENVVVSLDLSGD
                     ALHIRGYREETGKAPLRETLAAAIILRSGWKVGTPLVDPMCGSGTLLIEAAQMAANIA
                     PQLHRKHWGFNAWKGHQQAMWKAVLDEAFRNVELGAVGENCNSSLQKMFFGFDLDHRV
                     LTKAKQNAKNAGVDHLIQWQQGDVAALKNPIPEKIGTVVCNPPYGERLGTTPALIALY
                     SVFGQRLKQQFAGWNASIFSGEPELLNCLRLRSARQFKAKNGPLDCVQKNYSISEHNK
                     RSDLDENLQFEQNAQVAPDFANRLTKNIKKIEKWAKQQGIDAYRLYDADLPEYNLAVD
                     RYGDHIVVQEYQAPKNIDEQKARQRLLDAVSATLYVTGVETNKLVLKVRQKQKGTNQY
                     EKLANKGEYFYVNEYGAKLWVNLTDYLDTGLFLDHRLTRKMVGEMAKGKTFLNLFAYT
                     GSATVHAALNGAKSTTTVDMSNTYLNWAEQNLELNNLSLRHHRLFQADCLQWLAECRE
                     RFELIFVDPPTFSNSKRMENSWDVQRDHIELMKQLKRILTADGTIVFSNNKRGFKMDF
                     DGLAALGLTAENISHKTLPLDFERNPQIHNCWIVKNV"
     gene            12388..12867
                     /gene="trmL"
                     /locus_tag="PXD66_000938"
     CDS             12388..12867
                     /gene="trmL"
                     /locus_tag="PXD66_000938"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDL6319258.1"
                     /translation="MLDIVLYEPEIPQNSGNIIRLCANCGFRLHMIEPFGFGWDDKKL
                     RRSGLDYHEFVDIQKYKNFEDFLERAKPKRLFALTTRGEQNHSDVKYELGDFLMFGPE
                     SRGIPKPLLAEMPMSQKIRIPMCKDSRSMNLSNSVAVVVYEAWRQFGYQNAVAAQSI"
     gene            complement(12961..>13718)
                     /locus_tag="PXD66_000939"
     CDS             complement(12961..>13718)
                     /locus_tag="PXD66_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HDL6319259.1"
                     /translation="KWYFSIIDVIAILTESLDPSAYWRKLKQRLKAEGNETVTNCHGL
                     KMPSADGKMRLTDVADTEQLFRLIQSIPSPKAEPFKQWLAKVGYERLQEINDPSQSLD
                     RARENWQKMGRSEKWIQQRMMGQETRNKLTDYWKSSGVEQPQDFAFLTNVIHQEWSGL
                     SVQKHKSLKGLKSQNLRDHMSEAELLFTALAELSSRQIAESMQAVGVDENAQAGKQGG
                     NIAKNARQELENKTGKSVVTGENFLPPAKNEEE"
BASE COUNT         3959 a         2696 c         3193 g         3870 t
ORIGIN      
        1 ttaaaaagaa aatcaaaaag agaagtgaat tattcagcta acgctttacg ttgagcttca
       61 actagctggc taatgccttg cttcgctaaa tctagcaatg tcagtaattc ttggtgcgag
      121 aacggttcgc cttctgccgt gccttgtact tccactaaac gaccatcttc gaccatcacc
      181 acgttcatat cagtttcagc gttggaatct tccagatatt ctaaatcgca caccgcttcg
      241 cctgctacaa taccgacaga aattgctgcg actaaacctt tcattgggtt agtttttagt
      301 gttccattag ctactaactt attcattgca tcgtacaatg ctacgcacgc accggtaatt
      361 gaggcggtgc gagtgcctcc atcggcttga ataacatcac aatctacggt aacggtacgt
      421 tcgcctaagg cttttaaatc taccaccgca cgcagagaac gggcaatcaa gcgttggatt
      481 tccattgtgc gtccgccttg tttgcctttt gccgcttcac gctgagtgcg agagtgggtg
      541 gctcgtggca acataccata ttctgcggta acccaacctt gttgctgacc ttttaaaaag
      601 cgtggcacgc tttcttctac cgtagcgtta cacaatactt tggtttcgcc aaactcaatt
      661 aataccgagc cttcggcata gcgagtgtag ttacgggtaa ttttaatttc tctaacttgg
      721 ttgtttgctc gattatttgg acgcattttc ttttcctttt gctaaaattt ttaccatttt
      781 agcaaagaac acagtaaaat gtgatttttc tttagcaaag aatttacata gccctcgccc
      841 cttgtgggag agggacggat ttttcttcgt tcagaagaaa aatcagggag aggggtaagc
      901 ggttattttt atctcaaatt ttacaaattc ctccctctct ctgaaaaata tttctgaacg
      961 aaatattttt cggtctctct ccctcaaggg gagagagctt tacttataaa aacacaagga
     1021 ataccaatga tttatagtat gacagcattt gcccatttag aaatgaaaaa agagtggggc
     1081 aatgcggtgt gggaaattcg ctcggtaaat caacgctttt tagaaaccta ttttcgtttg
     1141 ccggagcagt ttcgcaacct agaaatgacc ttgcgtgagc gtttacgttc tgcactgaca
     1201 cgaggcaaag tagagtgtag cttacgcatt gatttaagca aaacccaaaa cagtgaaatt
     1261 gcgttaaaca aagaatatgc cgcacaggtg gttcaatcat taaaatggtt aaaagaaacc
     1321 gcaaatgaag gcgaaatcaa ccttgttgat gtgctacgtt tcccgggcgt ggtggataat
     1381 gaaagccaag atttagacca aattgctcaa gatttattag ccggctttga acaaattctg
     1441 gcagatttta tcgcaatgcg agccagagag ggcgagaacg tgcaagccct gatcttacaa
     1501 cgtttagaag caatttcagt tgaggctgca aaggtacaag cccaaatgcc ggaaattctg
     1561 caatggcaga aagatcgttt acaacaacgg ttcgatgaac tgaatttaca gcttgatcca
     1621 caacgcttag aacaagaaat ggtgttactt gcccaacgtg ttgatgtggc ggaggaatta
     1681 gaccgcttgc aattacacgt taaagaaacc accaatatcc tgaaaaaagg cggtgcggtt
     1741 gggcgtaaac tggattttat gatgcaagaa ctaaaccgtg aatccaacac gctcgcctca
     1801 aaatcgatta atgcggacgt gaccaattcg gcggtggagc tgaaagtgtt gatcgaacag
     1861 atgcgtgaac aaattcagaa tttagaatag gacagattat ggcaatgaat ttcaagcaag
     1921 tgagccagag ctatccaaat ctctgcgtgc cgctttctca gtattgttta attgaaatcg
     1981 caggggtaga tgctgagaaa tatcttcaag ggcaattaac ctgcgatgtg gcaaaaattt
     2041 cggtgggcga acatacgcta accagccatt gtgatccgaa aggcaaaatg agtgcgttac
     2101 tgcgtttgta tcgtgcagaa aatgagaaat ttatggcgat cattcaccgc agcttattgc
     2161 ctgaagccct gacgcaactt aaaaaatatg cggttttttc aaaagtgacc ttcacggaat
     2221 tagacacccc actttatggt atggcaggtg aagcagcatt tgcaaaatta agcgaaaata
     2281 tgaccgcttt gcgtttaacc acaggacagc ctcgggccat tgtttggggc gaagagcttg
     2341 aaacgactgc cgatgaacag ctctggacat taatggatat tcaagacggc attcctgttc
     2401 tcttgcaaca aaaccagttt gagctgattc cacaggcaac caatttacag gcacttgaat
     2461 ctgcgatttc attcaccaaa ggttgctaca tcgggcaaga aacggtagcg agagcgaaat
     2521 atcgtggggc gaataaacgt gctttattta cccttgccgg caactttgaa agcgaaatca
     2581 gcttaccgga agttgcttct gcgattgaaa tgcaactggg cgaaaattgg cgagcaaccg
     2641 gcactgtgtt gaccagtgcg actgatgaaa ataccctgtg ggtgcaagtg gtgctgaata
     2701 aagatgttga acctaacacc aagtttagag ccaatggtgt agcgttggaa atggtcgagt
     2761 taccttatag tttggcagaa aatgagtaaa acgaacggtc atcagggtat gaccgttttt
     2821 ttatatctga tcagggtgat gaatcatcac aaaacgttcc cacaactgct cttcggtttc
     2881 gatattatca ggatctttga taatgcagtt ggtaatcggg caaactttct ggcaagtcgg
     2941 cttttcataa tgcccgacac attcggtaca taacaccgga tcgatgatat aaatatcatc
     3001 gcccaccgaa atcgcctcgt tcgggcattc cggcaagcac atatcacagt tggtacattt
     3061 gtgggtaatt aataaagcca tagtttatat tgagaaaata aaaattaaac aagcggtcga
     3121 ttttgcaaaa aaatttgcaa atcttaccgc ttgtcactaa atgctaccta ttataaagca
     3181 aaataatttg aactacttca catttttgca atttggttgt ttatccgatt aagaaaattt
     3241 tttatgatgc catcgaaacg tttcgatgga gtattttcca acgtgatgga gcgaaaataa
     3301 aggggttagt atgaaaaaaa cgctaaatct aaatgcacaa ctttcgcaca gtattgcgac
     3361 actcgggcat accgatagtt taacggtttg cgatgccggt ttgccgattc caaacacaat
     3421 agagcggatc gatttggctt taaccgccgg tgtgccaagt tttttacaaa catttgatgt
     3481 agtagttagt gaattatttg ttgaacgagt gctacttgct gaagaaatta aacataaaaa
     3541 tccgcaagtg ttagcagctc ttttggagcg tttaacccaa ttagaacaaa ctcaacagaa
     3601 taaaattcaa attgattatg tgaaccacga aatctttaaa gagcagacac attacagtaa
     3661 agctgttgtg cgttcgggtg agtgttcacc gtatgccaat attattttat attcgggcgt
     3721 gccgttttag ggtggaatga tggaaacctt attaaaaatg agcgggatag ataaagaatt
     3781 tcccggcgtg aaagccttga gcaatgcgtg cttatctgtt tacggtgggc gagtaatggc
     3841 attaatgggc gaaaatgggg caggtaaatc caccttgatg aaagtattga cggggattta
     3901 tagcaaagat gccggctcta ttagctattt aggcaaagaa gttaccttta aaggcccgaa
     3961 agattcacaa gaagccggta ttagcattat tcaccaagag ctgaatttag tcggtaattt
     4021 aaccattgct gaaaatattt ttttgggccg tgagtccaaa accgcttggg gagctattga
     4081 ttggaaaaaa atgtatgccg aggcggataa actgcttgcc cgacttgggg taaaacacag
     4141 tagccatcag ctttgtgcgg agttatccat tggcgagcag caaatggtgg aaattgcgaa
     4201 agccctgagt tttgagtctc aagtaattat tatggacgaa ccgaccgatg ctttaaccga
     4261 tacagaaaca gcatcgttgt ttaaagttat tcgagagtta aaagcggaaa atcgaggcat
     4321 tgtttatatt tcccatcgtt taaaagagat ttttgaaatt tgtgatgatg tgacggtgct
     4381 aagagacggg caatttatcg gcgagcgagt aattgccgat attgatgaag atcagttaat
     4441 tgaaatgatg gttggtcgcc gtttggaaga gcaatatccg catttacacc aagagcgtgg
     4501 cgagttatta cttgaagtga agaatttaag tggaagtggg gtgaataatg tgtcattcca
     4561 attacacaaa ggtgaaattg tgggtatttc cggcttaatg ggggcagggc gtacagaatt
     4621 aatgaaggtg ctttatggtg cattgccgaa aacagccggc tcgatagagt taaatggaaa
     4681 agcgatttcc aaccattgtc cgcaagatgg gttgaataac ggcattgtgt atatttcaga
     4741 agatcgtaaa ggcgatggtc tgattttggg aatgtcggta aaagaaaata tgtctttgac
     4801 ctctctcgat catttctcca aagccggcag tattcgccac gatgcagaaa aattggcggt
     4861 cgatgatttt attgagatgt ttaacattaa aaccccaagt cgggagcaac aaatcgggct
     4921 gctttccggt ggtaatcagc aaaaagtggc gatcgctaaa gggttgatga cccgcccgaa
     4981 tgtgctgatt ttagacgagc cgacacgggg tgttgatgtg ggggcgaaaa aagagatcta
     5041 tcaattaatt aatgaattta aaaaagatgg gcttagtatt ttgatggttt cgtccgatat
     5101 gccggaagtg ttgggaatga gcgaccgcat tttagtgatg cacgaagggc aaatcagtgc
     5161 agaattttca cgagcagaag cgacacaaga aaagctactg gcagcagcta ttggcaaaag
     5221 tacaggatta aattaaggtg tgtttatggc aactcaaaca aagcagtttc aacttggcag
     5281 atttttaatc gagcaacgtt cttttgtagc gttatttatt ttaattgtca gtgtttcaat
     5341 gattaaccct gattttttca gcgtggataa tattttaaat attctacgcc aaacctcggt
     5401 gaatgcgatt attgcggtcg gaatgacctt tgtgatttta attgcaggca ttgatttatc
     5461 ggtcggttcg gttttagcct taaccggtgc ggttgcagcg accttagtcg gttctgaatt
     5521 gccgattttg ttggtgattc cgctggtttt attattaggg acggcatttg gtggtattag
     5581 tggggcgatt gttgctaaag gtaaagtgca ggcatttatt gcgactttgg tcacaatgac
     5641 cttattgcgt gggattacga tggtttatac cgatggcaga ccgattagca ccggcttttc
     5701 tgaccaagca gacgcctttg cgtttattgg gacaggctat ttatttggta ttcctgtgcc
     5761 gatttggatt atggcggtag tgtttgggtt agcttggtat attttgaaac acaccccgat
     5821 tgggcgttac atttacgctt taggtggcaa cgaatcggca acccagcttt caggcatcaa
     5881 tgtgaataaa attaagatct tcgtatttgc cgtgagtggt tttttatctg ctcttgccgg
     5941 cttaattgtg acttcacgtc tctcttctgc tcagccaacg gcaggtgtgt cctatgagtt
     6001 agacgcaatt gcagcggtag ttgttggtgg aaccagtcta atgggcggta aagggcgtgt
     6061 aatgggaacg cttgtcgggg cattaattat cggtttctta aacaatgccc ttaatttatt
     6121 ggatatttct tcatattatc agatgattgc taaagcgttg gttattttag cagctgtatt
     6181 agctgataat tatctaggca ctaaaaaagt ttaatctatc tttaaaggag atttctatga
     6241 aaaaattaac tacgattgca tcagcggtta tgttaagttt atcggttgca gccactgctt
     6301 cggcaaaaga gactatcgct cttgcagtat cgactttaga taacccattc tttgtgagcc
     6361 ttaaagacgg tgcacagaaa aaagcagatg aattaggcta caaattagtg gtgcttgatt
     6421 cccaaaatga cccggctaaa gagctttcca atgtggaaga cttaacggtg cgtggggcaa
     6481 aagtgttatt gattaaccca accgattcgg aagcggtcgg aaatgcggtg gctattgcaa
     6541 atcgcaacaa aattccggtt attacacttg accgtggtgc agcaaaaggc gaagtggtta
     6601 gccatatcgc ttccgataac gttgccggcg gcaaaatggc gggagatttc attgctcaga
     6661 aattaggctc aggggcgaaa gtgattcaat tagaaggcat tgccggcact tccgccgctc
     6721 gtgaacgtgg tgaaggcttt aaacaagcga ttgaagcaca taaattcaat gtattagcca
     6781 gtcaaccggc tgattttgac cgcactaaag gcttaaatgt gactgaaaac ttgctctcaa
     6841 gcaaagggga cgtacaagct atttttgctc aaaatgatga aatggcacta ggagctttgc
     6901 gtgcagtgag ttcagcgaag aaagaggttg taattgtggg ctttgacggc acagatgatg
     6961 gcgtaaaagc cgttgaaagc ggtaaacttg ccgcaacgat tgctcaacaa cctgaactga
     7021 ttggctcatt aggcgtagaa actgccgata aagtgctaaa aggcgaaaaa gtggaagcaa
     7081 aaattccggt tgatttaaag gttatttcaa aataattata cttgaagata gcgttcgtct
     7141 ctgacgaacg ctttgaatta ataaaatagc actcgtcaga gactagtgct atcagaatcc
     7201 tagttttgtt gtactaggta atttgagggc ttaatatgaa aaaactcacc gttctcggta
     7261 gcattaacgc tgatcacgtg atttccgttc cctattttgc caaaccgggg gaaacgctca
     7321 ccggacatag ctgccacatt gcttatggcg gtaaaggggc aaatcaggcg gtggcggcag
     7381 cacgtttggg agctaaggtt tctttcatcg gttgtattgg cagtgatgac atcggcaaag
     7441 caatgaaatc agcctttgag caagatggca ttgacactcg ctcgattaaa acgattccga
     7501 atgaaatgac cggcattgca atgattcaag tggcggaatc gggcgagaac agtattgtga
     7561 tttcagccgg agcgaacggt catttggatg aaacggttgt ggcaaagtgt gcagccgaaa
     7621 tcacacaggc ggattatttg ctaatgcagc tagaaacacc gttgccggcg attgtgcaag
     7681 cagcaaaatt ggctcagcaa aacggcacga aagtaatatt aaaccctgcg cctgctcgag
     7741 cgttacccga tgaactgtta aacttactgg atatgattac cccaaatgaa acggaagcgg
     7801 aaatattaac cggtgtgaaa gttgttgatg aagccagtgc gattcaagcc gcacaagtgt
     7861 ttcaccaaaa gggcatcgaa aaagtgttga ttacacttgg ctcaaaaggg gtatttgtaa
     7921 gcgaaaacgg tgtaggcaaa atcgttgcag gcttccgtgt tcaagcggtg gataccactg
     7981 ccgcagggga tacttttaac ggagcattag tgactgcaat gttagaagat aaaccgctgg
     8041 atcaagccat tcgttttgct cacgcagcgg cagcgattag tgtgactcgc aaaggggctc
     8101 aaccttctat tccgacccgt caggaaacac tggattttct aagccaacaa gcggtgagtt
     8161 tttaggaaaa ttttgcaaat ggcaacaatg aaggatattg cacggcttgc acaggtttcc
     8221 acctcgaccg tttctcacgt tatcaacaat agccgatttg tcagcgagga aattcgggaa
     8281 agagtgcaga gggtggtgaa agaactgaat tacacgcctt ctgcattggc tcgtagcctc
     8341 aaagtgaatg aaactaaaac catcggaatg ctggtaacgg caaccggcaa tccgtttttt
     8401 gcggaggtga tggcgggggt ggagcaatat tgccagcaac atcaatataa cctgattatt
     8461 gccactacca acggtgacac cgagcgtttg caacaccatt tgcaaatgct gatgcaacga
     8521 aaagtagatg gcttgttact gatgtgtggc gataccagat tgaatgccga tgagagcttg
     8581 aatattccgc tgccattggt ggtgttagac tggtggttta ccgagctcaa tgcagataaa
     8641 atctttgaaa attcaatatc cggcggctat ctcgcaacaa aaaccttaat tgaagcaggg
     8701 cattctaaga ttgcgattat tactggaaac ctaaaaaaat cattggcaaa aaatcgttta
     8761 gaaggctaca aaaaagcact tgccgaggcg aatattccac tcaatccaaa ctgggtgttc
     8821 gaaagccatt ttgattttgc cggcggggtt aatggaatga atgctttgct tgcccaaagc
     8881 gaaaagccga cagcggtatt tgcctgtagc gacaccattg ctctaggggc ttaccaagca
     8941 atctggcaac aaggtttacg aataccgcaa gatatttcga ttatcggcta tgacgatatt
     9001 gaactggcaa aatacctttc cccaccgctg agtaccatta gccagcccaa agcggaactc
     9061 gggcggcttg cggtggaaac tttgctaaaa cgaattcgtt ttaaatccac tgattttcaa
     9121 accataatgc ttgaacctga attagtttta aggcattcaa tccaaacaat aaacaaaaac
     9181 gctgaataat gtgaagttat tcagcgtttt ttatccgatt tcaattagcc tttttgctgt
     9241 tccaaataaa gcacggtggc ggcaactcgg gaatgcacgt ttaatttacg cagtaaattg
     9301 cggatatgca cttttacggt ttcttcggaa ataaagagct gagccgcaat ttgtttgttc
     9361 gacataccgg ttgcgagcca ttgtaaaaca tccagttcac gatctgttaa gcaacttaaa
     9421 ggatcatttt ctggctggcg gttgagtaaa tgttggcgaa cggtttcgct taataccact
     9481 tcaccggcag ccgctttacg gatattatct agtagttccg gggtgtcggt atcttttaat
     9541 aaatagccgt ccacaccagc atccattaat gcaaatacat cggattgctc gtctgacact
     9601 gttaaaacaa tgattctggc atctatacct tgagcgcgca atgagcgtaa agtatctagc
     9661 cctgaaagcc ctttcatatt taaatccaga ataatcacat ccggctcaag tcgtaatgca
     9721 agtttgatac cgtcggtgcc gttgcttgct tcacccacta cactaaattg gtgatctaat
     9781 tccaatagtt gacgcattcc ctgacgcatt aatgggtggt cgtcaatgag taaaatttga
     9841 gttggagctg tcatttattt ctcctttcct ttctcttaat cggttaattt tgcaattatt
     9901 aattcaaaat agcaagttta gggatttact aaatgccgct tttttgatct tagctaattt
     9961 ttaactaaaa aagaaattgc aaaactttgc aagaaaaaga ccgcttgttc ggctataatt
    10021 actgccaatt tttatttcaa gtgtgaaaag aatgacaaat caaacaacct attttgccac
    10081 ggcagcccgt ggttttgagg aaatactaaa aaccgaactg gagcagattt gtgcggcaga
    10141 atgcaaagtc gctcaaggcg gtgtgcattt taccaccacg caaaaagggg cttatcaggc
    10201 attattacac agccgtttag cctctcggat cttactgccg ctcatcagca ccaaaatttt
    10261 tagtgatagc gatttatatg cttccatcgt gtcatttaac tgggcggatt tattcgatcc
    10321 ccgtgatacc ttttacattg atttcagcgg cactaaccga gaaatccgca acacccaatt
    10381 tggggcaatg cgagtgaaag acggcattgt ggattacttt gaacgcaaag gttttgcccg
    10441 cccaacagta gataaagata atccggatgt tcgcattcat gtttatttgg atcgtgaaaa
    10501 tgttgtggtt tcgcttgatt taagtggaga tgccttacat attcggggct atcgggaaga
    10561 aaccggtaaa gctccgttgc gtgaaacatt ggcggcagcc attattttgc gttccggctg
    10621 gaaagtcggc acgccattgg ttgatccgat gtgtggttca ggcacgttac tgattgaagc
    10681 agcacaaatg gcagcgaata ttgcccctca acttcaccgt aaacattggg gctttaatgc
    10741 gtggaaaggg catcaacaag cgatgtggaa agccgtgtta gatgaggcat tccgaaatgt
    10801 tgaattgggt gcggtagggg agaattgcaa ttcgtcccta caaaaaatgt tcttcggttt
    10861 tgatctcgat catcgtgtgc tgactaaagc aaagcaaaat gcgaaaaatg caggggtaga
    10921 tcatctcatt caatggcaac aaggtgatgt ggctgcgtta aaaaatccga ttccggaaaa
    10981 aatcggcacg gtggtgtgta acccccctta tggtgaacgt ttaggcacaa cgccggcgtt
    11041 aattgctctt tattcggtgt tcgggcaacg cctaaaacag caatttgccg gttggaatgc
    11101 ctcaattttt agcggcgagc ctgaattgtt aaattgcctg cgtttacgtt cagcccgtca
    11161 attcaaagcg aaaaacggtc cgttggattg tgtgcagaaa aattactcca tcagcgagca
    11221 taacaagcgg tcagatttag atgaaaattt acaatttgaa cagaatgctc aagtcgctcc
    11281 ggattttgca aatcgcttaa ccaaaaatat caaaaagatc gaaaaatggg cgaaacaaca
    11341 aggcattgat gcctaccgtt tatacgatgc cgatctgcct gaatataatt tagcggtgga
    11401 cagatatggc gaccacattg ttgtgcagga ataccaagcc ccgaaaaata ttgatgagca
    11461 aaaagcccgc caacgcttgt tagatgcagt ctctgctacg ctttatgtga caggtgttga
    11521 aactaataaa ttagtgttga aagttcgcca aaaacaaaaa ggcacgaatc agtacgaaaa
    11581 acttgccaat aaaggcgagt atttctatgt gaacgagtat ggggcgaaac tgtgggtgaa
    11641 tttaaccgat tacttggata cgggtttgtt cctagaccat cgcttaaccc gtaaaatggt
    11701 gggcgaaatg gcaaaaggca aaacattcct gaatctgttt gcctacacgg gttcagctac
    11761 ggttcacgct gctcttaatg gggcaaaatc gaccactaca gtggatatgt cgaacaccta
    11821 tttgaactgg gcggagcaaa atttagagct gaataattta tcacttcgcc atcatcgttt
    11881 gttccaagcc gattgcttgc aatggctggc agaatgccgt gagcgatttg agctgatttt
    11941 tgtcgatccg ccgactttct ccaactcaaa acgaatggaa aacagttggg acgtgcaacg
    12001 agaccatatt gagttaatga aacagcttaa acgtatttta accgctgatg gtaccattgt
    12061 gttctcgaac aataagcgtg gctttaaaat ggattttgac ggtttggcag cattaggctt
    12121 aacggcggaa aatatttcgc ataaaacctt accgttggat tttgaacgca atccgcaaat
    12181 ccataattgt tggattgtga agaatgtata gataataccc tcgacccttg tgggagaggg
    12241 atagattttt cttcgttcag aagaaaaatc agggagaggg taagaagtta ctgatgaaag
    12301 agttatttcc ccctctctct gaaaaatatt tctgaacgaa atatttttcg gtctctctcc
    12361 cacaagggga gagagtaagg ctaaaaaatg ttagatattg ttttatacga acctgaaatt
    12421 ccgcaaaaca gcggaaatat tatccgcctt tgtgccaact gtggctttcg tttgcatatg
    12481 attgaaccgt tcggctttgg ttgggacgat aaaaagctcc gccgttcggg gctggattat
    12541 cacgaatttg tggatattca aaaatataaa aattttgaag atttccttga gcgtgctaag
    12601 cctaaacgct tgttcgcctt aaccaccaga ggggagcaga accacagtga tgtgaaatat
    12661 gaacttggcg attttttaat gttcgggccg gaaagcagag gcattccaaa gccattatta
    12721 gcggaaatgc cgatgtcgca aaaaatccgc attccgatgt gcaaagacag ccgcagtatg
    12781 aatttatcta actcggtggc ggtggttgtg tatgaggcgt ggcgacaatt cggctaccaa
    12841 aatgcggtag cggcacaatc tatttaactt gcaagcggtg tgattttggc tattttttgc
    12901 aagtttgtgg tcaaggttgg gggaggctcg accttgacta attaagaaca tctattgtgt
    12961 ctattcttct tcatttttag ctggaggtaa aaaattctct ccggtaacaa ctgatttacc
    13021 tgttttattc tccaattcct gacgggcatt tttggcaata ttgccacctt gttttcctgc
    13081 ttgggcattt tcatcaaccc caactgcctg catactttct gcaatttgcc gactggaaag
    13141 ttcagcaagt gctgtaaata acaattcagc ttcgctcata tgatctcgca gattttggct
    13201 ttttaaacct ttaagcgatt tatgcttttg tacagatagt ccgctccatt cttgatgaat
    13261 cacattggtt aaaaatgcaa aatcttgtgg ctgttcaacc cctgacgatt tccaataatc
    13321 ggtcagttta tttcgggttt cttgtcccat cattctttgc tgaatccatt tttcacttcg
    13381 tcccatcttc tgccaattct cccttgctcg atctagactt tgacttggat cgtttatttc
    13441 ctgtaagcgt tcataaccta cttttgctaa ccattgtttg aagggctctg ctttcggact
    13501 agggatagat tgaataagtc ggaaaagttg ttcggtatct gctacatcag tcaaacgcat
    13561 ttttccgtca gcagagggca ttttcaaccc gtgacaattt gtcacggttt cattgccttc
    13621 tgcctttagc ctctgtttta gcttacgcca ataagcagag ggatctaggc tttcggttaa
    13681 aatagcaatc acatcaataa tagaaaaata ccatttat
//
LOCUS       DANLXF010000052        13638 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.052, whole genome shotgun sequence.
ACCESSION   DANLXF010000052 DANLXF010000000
VERSION     DANLXF010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13638
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.052"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..409)
                     /gene="fur"
                     /locus_tag="PXD66_000940"
     CDS             complement(<1..409)
                     /gene="fur"
                     /locus_tag="PXD66_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HDL6319260.1"
                     /translation="MSEANIKRLKEVGLKVTEPRLTILALMQDIRDEMQHFAAEDIYK
                     ILLEKGSDIGLATVYRVLNQFEEAGILTRHNFDANKAVFELNMNHEHDHIICMDCGKV
                     FEFKDPDMERRQREISEKHGMELTNHSLYLYGKC"
     gene            complement(445..969)
                     /gene="fldA"
                     /locus_tag="PXD66_000941"
     CDS             complement(445..969)
                     /gene="fldA"
                     /locus_tag="PXD66_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HDL6319261.1"
                     /translation="MAVVGLFYGSDTGNTENISKMIQKKLGAELIDIRDIAKSTKEDI
                     EAYDFLMFGIPTWYYGESQADWDDFMPTLKEIDFNGKVVAIFGCGDQEDYAEYFCDAM
                     GTVRDVVEPNGGVIVGHWPTAGYTFEVSQALVDDDTFVGLCIDEDRQPELTTERVNKW
                     CEQLKEEMCLDQLA"
     gene            complement(975..1250)
                     /gene="ybfE"
                     /locus_tag="PXD66_000942"
     CDS             complement(975..1250)
                     /gene="ybfE"
                     /locus_tag="PXD66_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HDL6319262.1"
                     /translation="MAKQDSDCITLDLFATVPKVGRPRTNPLDREQQLRINKRNQLKR
                     DKSSGLKRVELKLHSNLVKLLEEQASEQGVSRGQLIETILNNYLTNR"
     gene            1491..2477
                     /locus_tag="PXD66_000943"
     CDS             1491..2477
                     /locus_tag="PXD66_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL6319263.1"
                     /translation="MSNIHLAIAADFTLAEKLLEALANQDLGIENISAVELEPFGEEQ
                     NLRFGAKAVAQYALDEVNWAEFSHVLFAGKMAQAEVLAEAVKAGCVILDLYGITALIG
                     DVPVVVPSVNDENVANLRERNIVALANPQISQLALALKPLLNEPLKQVWVTSLLPSAY
                     FGDEKVKELAGQTARLLNGIPFDENAERIAFDVVPANVQGEEKSLPFSKAFELQIAKV
                     LPNLTACTTFHSVQVPVFYGVSQMVSVQSEYQLDANALSEAWKAENWLNFNENSVITA
                     VKNGENEEQNALEISQLLAKSEQEVQFWTVADEQRFSLAFLAVKLLKLVLAY"
     gene            2574..3869
                     /locus_tag="PXD66_000944"
     CDS             2574..3869
                     /locus_tag="PXD66_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL6319264.1"
                     /translation="MLEKLFQLKAKGSNVKTEIIAGITTFFTMVYIVFVNPSILGVAG
                     MDTQVVFVTTCLIAAFGTIAMGLFSNLPIALAPAMGLNAFFAFVVVQKLGYSWQVGMG
                     AIFLGSVGLFLLTVLQVRYWFMSAIPLGLRVGIGAGIGLFIALIGFKNMGLVVPNPAT
                     LVALGDLHDPKVLMGILGFFIIVVLAAKGIHSGVLISIVIVTALALAFDPAVSFNGIM
                     SMPPSLGAVVGQVDIAGAFDVALLGMIFSFMLVNLFDSSGTLIAVTTKAGFADEQGRF
                     PKMKQALLVDSTAAMAGSYMGTSAISTYIESGSGVSVGGRTGLTAVVVGILFLLTIFF
                     SPLAGLVPAYATAGALVFVGILMASSLIEVKWDDLTEATPAFITTAMMPFTYSITEGI
                     AFGFISYCVMKAFTGRWKEINLSVLVVSLLFIAKFVWVG"
     gene            3989..4357
                     /locus_tag="PXD66_000945"
     CDS             3989..4357
                     /locus_tag="PXD66_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/TusD sulfur relay family protein"
                     /protein_id="HDL6319265.1"
                     /translation="MSNSVQKLLFIINSAPYGNETFFSALRLALQIQEQHKANTEIKL
                     FLMSDAVTAGLAKQNPAEGYHIQQMLEILTAQGANVKLCKTCTNARGITDLPLADGVE
                     IGTLIELADWTIQADKVLNF"
     gene            4357..5550
                     /locus_tag="PXD66_000946"
     CDS             4357..5550
                     /locus_tag="PXD66_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL6319266.1"
                     /translation="MQNIDVVVIGAGAAGLFCAAQLGQAGKKVVVLDSGKKVGRKILM
                     SGGGFCNFTNLDVSAGNYLSQNRHFVKSALSRYTQWDFISLVSHYGISYHEKELGQLF
                     CDESSQQIVDLLQAECNKGKVEILLRQAVISVEKVANFFEISTACGKFQSEHLVIATG
                     GLSMPALGASPFGYQLAEQFGIPVVPPRASLVPFTWKESDKYFAELSGIALPITVSAN
                     GKSFSNQMLFTHRGLSGPAILQISNYWELGETVEIDLLPSQSITDILSELRQSSPKLQ
                     LKTVLSRYLPKKLVEVWLEQKLLTDKVIADLSKKDLTLLEQFIHHWHFLPNGTEGYRT
                     AEVTKGGVDTDYISSKTMEAKNTAGLYFIGEVLDVTGWLGGYNFQWAWSSAFACAEGI
                     IEKSK"
     gene            complement(5621..5839)
                     /gene="infA"
                     /locus_tag="PXD66_000947"
     CDS             complement(5621..5839)
                     /gene="infA"
                     /locus_tag="PXD66_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDL6319267.1"
                     /translation="MAKEDCIEMQGTILETLPNTMFRVELENGHIVTAHISGKMRKNY
                     IRILTGDKVTVEMTPYDLSKARIIFRAR"
     gene            complement(5949..6608)
                     /locus_tag="PXD66_000948"
     CDS             complement(5949..6608)
                     /locus_tag="PXD66_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HDL6319268.1"
                     /translation="MYISREKITKTLLLAGITLLCCGIVAVILMGVNQFKLGSQLASI
                     NQVSNLSHLLVRQQANIFSFLLVKNAKTDELVDALDSFAKEDFIIDANIYSPNGVLLA
                     QSQNAKDLKVLLDSEQSAKTQQIVEPIFSQQALVGFLRVTFDAEYGQTTKARVEQQFH
                     QLYGEIIILLLTGILLASSINYYFRRKIHHVQISPKPLMSQVKTQTQRFHSRRRWIKR
                     K"
     gene            6694..7557
                     /gene="serB"
                     /locus_tag="PXD66_000949"
     CDS             6694..7557
                     /gene="serB"
                     /locus_tag="PXD66_000949"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HDL6319269.1"
                     /translation="MTNTIFIYSKTLNEREIEQFSQQTAAILLKQAVYLDYKIAFFST
                     NLTACELRETAQKINADISDLTIIPTLNQGGLLLMDMDSTAIKIECIDDIAKLAGTGE
                     VVSAITASAMRGELDFEQSLRKRVGTLENASESILQKVRENLPLMDGFEQMVAILKEH
                     GWKLAIASGGFDYFADYLKQKYGLNFAVSNQLEIIDGKLTGKVLGKVVDAQHKADTLQ
                     NLATTFNIPPNQWVAVGDGANDLPMLKTASLGVALHAKPKVQAQADFVVNFGDLTALC
                     LLLNANHLFQH"
     gene            7573..8064
                     /locus_tag="PXD66_000950"
     CDS             7573..8064
                     /locus_tag="PXD66_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HDL6319270.1"
                     /translation="MPSFDIVSEITMHEVRNAVENANRVLSTRYDFRGVEAVIELNEK
                     NESIKLTTESDFQLEQLIEILIGSCVKRGIEHSSLDIPTESEHHGKLYSKEIKLKQGI
                     ESDMAKKITKLIKDSKIKVQAQIQGEQVRVTGKSRDDLQAAIQLVKGADLGQPFQFNN
                     FRD"
     gene            8149..8325
                     /locus_tag="PXD66_000951"
     CDS             8149..8325
                     /locus_tag="PXD66_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5363 domain-containing protein"
                     /protein_id="HDL6319271.1"
                     /translation="MMSEQTEKKGWFKKALEKYDAFCDELGVNQGACRGCVPVVKFDP
                     EPEKEQPEKSENKT"
     gene            complement(8373..8669)
                     /locus_tag="PXD66_000952"
     CDS             complement(8373..8669)
                     /locus_tag="PXD66_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319272.1"
                     /translation="MKKHHIIILTLASASVVACSSNTPVQVVNGDKNIDYSQPKTIYI
                     SETNTQPPALTKQEKIKPSNATALCFNGSYSTDIQNPCKKDGGVKQYFAHHHAN"
     gene            complement(8746..9714)
                     /gene="mdh"
                     /locus_tag="PXD66_000953"
     CDS             complement(8746..9714)
                     /gene="mdh"
                     /locus_tag="PXD66_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDL6319273.1"
                     /translation="MCQSKKVALLGAAGGIGQSLALLLKLNLPAKSELSLYDISPVTP
                     GIAVDLSHIPTDVKVTGFAGEDPTEALKDADVVVISAGVARKPGMTRADLFNTNATIV
                     HNLVEKAAKVCPKACIAIITNPVNTIIPIAAEVLKKAGVYDKNKLFGVTTLDVIRANT
                     FVAEAKDVNVKYVRVPVIGGHSGTTILPLLSQATVNGLKLEFTQEQIEQLTHRIQNAG
                     TEVVEAKAGGGSATLSMAQAGAEFALGLVRALIGEDVIRYAYVDNANGETSPAFFAYP
                     IRLGTNGVEKVLPIGNLSEFEKDQLEQLIPVLNDEIQLGQTFNKNA"
     gene            9910..10371
                     /gene="argR"
                     /locus_tag="PXD66_000954"
     CDS             9910..10371
                     /gene="argR"
                     /locus_tag="PXD66_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HDL6319274.1"
                     /translation="MDKIDSLSEAFKSLLKEEKFGSQSEIVAALQEMGFDNINQSKVS
                     RMLSKFGAVRTRNPKMEMVYQLPVELSVPTTSSPLKNLVVDIDHNDALIVVKTSPGAA
                     QLIARLLDSMGKAEGILGTIAGDDTIFITPVRNVQIEKLVKNVTQLFETSL"
     gene            10421..11317
                     /locus_tag="PXD66_000955"
     CDS             10421..11317
                     /locus_tag="PXD66_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HDL6319275.1"
                     /translation="MHIFITGGTGFIGRELIQQLKSENHHFTLLTRDTEAKSDDLQIN
                     YCYSLDELVDLNDFDAVINLAGEPIFDKRWTDEQKQKLIESRVNLTNKLSQLMNASSN
                     PPHTFLSGSAIGIYGDLPPSANFYTESTACYQDCFTNRLCQQWEAAALKAASDKTRVC
                     LIRTGTVFSPEGGALKKILPLYRCGLGGKLGSGKQYWAWISRRDHVQAVIFLLKNANC
                     SGAYNFVAPNPVRNEEFNRILANAVNRPAFFTVPEFALKLGLGERACLVLDNQQLIPE
                     KLLAASFVFQDPILTENVFLNP"
     gene            11362..13560
                     /gene="uvrD"
                     /locus_tag="PXD66_000956"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             11362..13560
                     /gene="uvrD"
                     /locus_tag="PXD66_000956"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HDL6319276.1"
                     /translation="MDFSLLLDGLNDKQREAVAAPIGNYLVLAGAGSGKTRVLTHRIA
                     WLIGVENVPESNILAVTFTNKAAAEMRHRIEYTLSQSGDNRLFGMWVGTFHSIANRLL
                     RSHYLDADLPQDFQIMDTEDQQRLLKRLLKLHNIDEKHFPPKHVAWYINAQKDKGKRA
                     KDIDHYNDPNEKKLVEIYQIYQDACDRAGLLDFAELLIRAYELFKTKSTILQRYQQRF
                     QQILIDEFQDTNNIQYDLIRLLAGDVGKVMIVGDDDQSIYGWRGAQVENIQRFLNDYD
                     NAQTIRLEQNYRSTGNILSCANELIANNDNRLGKNLWTDSGDGEPVDIYCAFNELDEA
                     RFVASQIKQWKEDEGDLAECAVLYRSNSQSRVIEEALIQANIPYRIYGGMRFFERQEI
                     KDALAYLRLIANRQDDAAFERVINTPTRGIGDRTLDTLRQITRGRQITLWQAIQVAVQ
                     EEQLSGRATSSLLRFVELINALDQETEQMSLAEQTDFVIKKSGLYEMYKQEKGEKGEV
                     RIENLEELVTATKQFVKPDEAEELTDLTAFLTHASLEAGEAQASQHEDYVELMTLHSA
                     KGLEFPRVFIVGVEEGIFPSGMSFDEGRLQEERRLAYVGITRAKKKLTISYAESRRLY
                     GKEERHVPSRFIAELPEEHIREVRLRGSINRAASFNQANSFAKNPTKTTASFLNDDTW
                     KMGQKVNHAKFGQGTIINVEGSGEATRLQVAFVGNGIKWLIAKMANLERI"
BASE COUNT         4014 a         2609 c         2941 g         4074 t
ORIGIN      
        1 tgcatttacc atacagatat aaactgtggt tagtcaattc cattccgtgt ttttcactga
       61 tttcacgttg gcgacgctcc atatcaggat ctttaaattc gaacacctta ccacaatcca
      121 tacaaataat atggtcgtgt tcgtggttca tatttaattc gaatactgct ttattcgcat
      181 cgaaattatg gcgggttaaa atgccggctt cttcaaattg gtttaagaca cgataaaccg
      241 ttgctaaacc aatgtctgag cctttttcta acagaatctt ataaatatct tctgctgcaa
      301 aatgttgcat ttcatcacga atatcttgca ttaatgcaag gatagtcaaa cgaggctcgg
      361 ttacttttaa gcctacctct tttaaacgtt taatatttgc ttcagacata tatgccactt
      421 atttatcttt atccaaaata gttattatgc gagttggtct aagcacattt cttctttcaa
      481 ctgctcgcac catttattaa cacgttcagt ggttaattca ggctgacgat cttcatcaat
      541 acataagccg acaaaagtat catcatcaac taaggcctga gaaacctcga aagtgtaacc
      601 ggcagttggc caatgaccaa caatcacacc accatttggc tctaccacat cacgcacagt
      661 acccattgca tcacagaaat attctgcata atcttcctga tcaccgcaac caaaaattgc
      721 aacgactttg ccgttaaaat caatctcttt tagggtaggc ataaaatcgt cccaatcggc
      781 ttgcgattca ccataatacc aagttgggat cccaaacatc agaaaatcgt atgcttcaat
      841 atcttctttt gtgcttttgg caatgtctcg aatatcaatt aattcagccc ctaatttttt
      901 ctgaatcatt tttgaaatat tttctgtatt gcctgtatcg ctaccgtaaa ataaacctac
      961 aactgccatt gctactacct atttgtgaga taattattta gaattgtttc aattaactgc
     1021 ccgcgtgaaa caccttgttc agacgcctgt tcctctaata actttactaa attcgaatgc
     1081 agttttaatt ctacccgttt taaaccggaa gatttatctc gttttagttg gttacgttta
     1141 ttgattcgaa gttgttgttc tctatcaaga ggatttgtac gaggacgccc aacttttggt
     1201 acagtcgcaa acagatctaa ggttatacaa tctgaatctt gttttgccat tttgttttac
     1261 tattgggctt tagctaatcc tcagttttaa tgagaatgat aagctacttt taaagtgtga
     1321 actatatcaa aagttcaatc atttgaaaag taaattacag gcaaaacaag cggtgttttt
     1381 aggtttaaaa tttgcaaaaa ccataaatta cacattatga gttatgattt ttctgaagcg
     1441 tgaaattagc ttataatgaa accgaataac gttatgaaaa aggagatagt gtgtcgaata
     1501 tccatttagc gattgccgct gattttacgc tggcagaaaa attacttgaa gcattagcaa
     1561 atcaagattt agggattgaa aatatttcag ccgttgagct tgagccgttt ggtgaagagc
     1621 agaatttacg ctttggggca aaagcagtcg cacaatatgc gttagatgaa gtaaattggg
     1681 cggaatttag ccatgttcta tttgccggca aaatggcaca agcggaagtg ttggcagaag
     1741 cggtgaaagc cggctgtgtg attttagatt tatacggcat taccgcactc atcggcgatg
     1801 taccggtagt tgtgccaagt gtaaatgatg aaaatgtcgc aaatttgcgt gagcgaaata
     1861 tcgttgcttt agccaatccg caaatttcac aactggcgtt agccttaaag ccattgttga
     1921 acgagccgtt aaaacaggtg tgggtaacct cacttttacc gtctgcctat tttggcgatg
     1981 aaaaagtcaa ggagcttgcc ggacaaactg cccgtttgtt aaacggcatt ccgtttgatg
     2041 aaaatgcgga acgcattgcc tttgatgttg tgccggctaa tgtgcaagga gaagagaaaa
     2101 gtctgccgtt ctcaaaagcc tttgaactgc aaattgcaaa agttttacca aatttgaccg
     2161 cttgtactac cttccacagc gtgcaagtgc cggtctttta tggggtttcc caaatggtgt
     2221 cggtgcagtc tgaatatcag ttagatgcta atgcgttaag tgaagcttgg aaagccgaaa
     2281 attggctgaa ttttaatgaa aattcagtga ttacagcggt gaaaaatggc gaaaacgaag
     2341 agcaaaatgc gttagaaatc agtcagttat tggcgaaatc cgaacaggaa gtgcagttct
     2401 ggacggtcgc cgatgaacag cgtttcagtc tggcattttt agcggtgaaa ttactaaaat
     2461 tagttttagc gtattaagtg cttgcaaaag tatttattcc gtgataaaaa tcgtcaacgc
     2521 ccgagcaacc gattgcttgg gcgttgtcat tttgtttaaa caccgaaggt cttatgttag
     2581 aaaaattatt tcagctcaaa gcaaaaggca gtaatgtaaa aaccgaaatt atcgccggta
     2641 ttactacctt tttcacaatg gtttatattg ttttcgtcaa tccgtctatt ttaggcgtgg
     2701 cgggaatgga cacccaagta gtgttcgtga ctacttgttt aattgccgct tttggtacta
     2761 tcgcaatggg gttattcagt aatttaccga ttgctcttgc accggcaatg ggcttaaatg
     2821 ccttttttgc attcgtagtg gtgcaaaaat taggttactc gtggcaagtg ggtatggggg
     2881 cgattttctt aggctctgtc ggcttattcc tattaactgt attacaagtt cgttactggt
     2941 ttatgtctgc gattccactt ggtttacgtg taggtattgg tgccggtatc ggtttattca
     3001 ttgccctaat cggttttaaa aatatgggct tggtggtgcc aaatccggca acgcttgtgg
     3061 cactaggtga tttgcacgac ccgaaagtgt taatggggat tttaggcttt ttcatcattg
     3121 ttgtattagc ggcgaaaggt attcactcag gcgtgttaat ttcaattgtg attgttaccg
     3181 ctcttgcctt agcctttgac ccggcggtga gctttaacgg cataatgtct atgccaccaa
     3241 gccttggggc agttgtgggg caggttgata ttgccggtgc ttttgatgtt gccttactcg
     3301 ggatgatttt ctcctttatg ttggtaaacc tgtttgactc ttccggcacc ttaattgcgg
     3361 tgactaccaa agcaggcttt gcggatgaac aagggcgttt cccgaaaatg aaacaagctc
     3421 tgctagtaga tagtaccgct gcaatggcag gctcttatat gggaacttca gcgatttcta
     3481 cctatattga aagtggctcc ggcgtttcag ttggcggtcg taccggctta actgcggtgg
     3541 tagttgggat cttattccta ttaaccattt tcttctcacc acttgcaggg ttagtacctg
     3601 cctatgcaac tgcgggggca ttggtatttg taggaatttt aatggcatct agcttaattg
     3661 aagtaaaatg ggacgattta actgaagcta cgccggcatt tattactacc gcaatgatgc
     3721 cgtttactta ctccatcact gaagggattg cattcggctt tatcagctac tgcgtaatga
     3781 aagcctttac agggcgttgg aaagaaatca acctttctgt tttagtggtt tcacttctct
     3841 ttattgctaa atttgtgtgg gtgggttaag ctctaccccc ctctccctga tttttcttct
     3901 aaacgaagaa aaatctatcc ctctcccaca aaggggagag ggtatttgat tcattatcta
     3961 tgtgtttcgt gtattccgag gtaaaacaat gtctaattca gttcaaaaac ttctttttat
     4021 catcaattct gctccttacg gcaatgaaac gtttttcagt gcgttgcgtt tggcattaca
     4081 aattcaagaa caacataaag ccaatacgga aattaaactt ttcttaatgt cggatgcagt
     4141 caccgcaggg ctggcaaagc aaaatccggc agagggttat catattcagc aaatgctgga
     4201 aattttaacc gcccaagggg caaacgtaaa attatgcaaa acctgtacta atgctcgagg
     4261 gattacagat ttgcctttag ccgatggggt agagattggc acattaattg aactggcaga
     4321 ttggacaatt caagcagata aggtactgaa tttttaatgc aaaatattga tgttgtggtg
     4381 attggtgccg gtgcggcagg gctgttttgt gctgcccaac tggggcaagc tggcaaaaaa
     4441 gtggtggtgt tagacagcgg taagaaagtc ggacgtaaaa ttttaatgtc tggcggtggt
     4501 ttttgcaatt ttacgaattt agacgtaagt gccggaaatt accttagcca aaaccgccat
     4561 tttgtgaaat ccgccctttc acgttatacc caatgggatt ttatttcgct tgtcagccac
     4621 tatggcatta gctatcacga aaaagagcta gggcagttgt tttgtgatga aagctcgcaa
     4681 caaattgtcg atttactaca agcagaatgc aacaaaggca aggttgagat tctgttacgg
     4741 caagcggtca tttctgtgga aaaagttgca aatttttttg aaatttcgac cgcttgtggc
     4801 aagttccaat ccgaacactt agtgattgca accggagggc tttcaatgcc ggctctcggg
     4861 gcaagccctt ttggctatca acttgccgag cagtttggta ttcctgttgt gccaccacgt
     4921 gcaagccttg tgccttttac ttggaaagag agcgataaat attttgccga gctttccggc
     4981 attgcactgc cgattactgt ttccgccaat ggcaaaagtt ttagcaatca gatgttgttt
     5041 acgcacagag gcttatccgg cccggcaatt ttgcagattt ccaattattg ggaattgggg
     5101 gaaacggtag agattgatct gctaccttcg cagtccatta ccgatatatt gagcgaacta
     5161 cgccaatctt cgccgaaatt acagctaaaa accgtattat ctcgttattt acctaaaaaa
     5221 ttggttgaag tttggctgga acagaaattg ctgaccgata aagtgattgc cgatttgagt
     5281 aaaaaagatc tgaccttatt agagcaattt atccaccatt ggcatttctt gccaaacggc
     5341 acggaaggct atcgtacagc ggaagtaacc aaaggcggtg ttgataccga ctatatttca
     5401 tctaaaacga tggaagcgaa aaatacagca gggctttatt ttatcggcga agtgctggat
     5461 gttaccggct ggcttggtgg ttataatttc caatgggcgt ggtcatctgc ttttgcctgt
     5521 gcagaaggga ttattgaaaa aagcaaataa ctaaaaaagc aaataactaa aaaaccaaac
     5581 aaaaaggcga acacaattgt tcgccttatt ttatgctgaa ttatcttgca cggaaaataa
     5641 tgcgtgcttt gcttaaatcg tatggagtca tttcaaccgt tactttatca ccggttaaaa
     5701 tacgaatgta gtttttacgc attttgcctg aaatatgtgc ggtaacaata tgaccgtttt
     5761 ctaattccac acggaacatt gtatttggta aggtttctaa aatcgtacct tgcatctcaa
     5821 tgcaatcttc tttagccatt tattcctctt taaagtttta atttttatat gatgccattc
     5881 ggcgaaaaac ggcggatatt atacgcttat tcgctcaaat tacaatcgtt tatccatttg
     5941 agtgtaaact atttacgctt aatccaacgg cgacgtgagt gaaaacgctg ggtttgggtt
     6001 tttacttgac tcataagtgg ttttgggctt atctgtacat gatgaatttt acgacgaaaa
     6061 tagtagttaa tgctacttgc caacaatata cctgtgagta gcaaaataat tatttcccca
     6121 taaagttgat gaaattgttg ctctactcgt gctttagtag tttgcccata ttctgcatca
     6181 aacgtgacgc gtaaaaagcc aaccaacgcc tgttgagaaa atattggctc aacaatttgc
     6241 tgagttttgg cagattgctc tgaatcgagc aatactttta aatctttcgc attttggctt
     6301 tgagccagta gcacgccatt tggtgaatag atattggcat caataataaa atcttctttg
     6361 gcaaaactat ccaacgcatc aaccagctca tcggttttgg catttttaac taacaaaaac
     6421 gaaaaaatat ttgcttgctg gcgaaccaat aggtgtgaaa ggtttgatac ttgattaatg
     6481 ctggccaatt gtgagcctag cttaaattgg tttacgccca ttaaaataac cgccacgatc
     6541 ccacagcaga gtagcgtaat ccctgccagc aaaagcgttt tcgttatttt ttcacgacta
     6601 atgtacatcg aaattccctc aaaatgatgc gtgattgtag cacgcttttc tgttagatta
     6661 gtgcagtaaa tttaaacaac ctaaaataag acaatgacaa acacaatttt tatctattca
     6721 aaaaccttaa acgagcgtga aattgaacag ttttcacaac aaacggcagc aattttgctg
     6781 aaacaagcgg tctatttaga ctacaaaatt gcattttttt ccacaaattt aaccgcttgc
     6841 gagctgcgtg aaacggcaca gaaaatcaat gcggatatta gcgatctcac tattattcca
     6901 accttaaacc aaggcggttt gctattaatg gatatggatt ccaccgctat taaaattgaa
     6961 tgtattgatg atattgccaa acttgccggc accggcgagg tggtgtccgc cattactgcc
     7021 agtgcaatgc ggggcgagct ggatttcgag caaagtttac gcaagcgagt cggcacgttg
     7081 gaaaatgcat cggaaagcat tttgcaaaaa gtgcgggaaa atctaccgct tatggacggc
     7141 tttgagcaga tggttgctat tcttaaggaa cacggctgga aattagcgat tgcgtccggt
     7201 ggatttgatt acttcgccga ttacttaaaa caaaaatacg ggttgaattt tgcggtctct
     7261 aaccaactgg aaattattga cggtaaatta accggcaagg tgttaggcaa agtagtggat
     7321 gctcaacata aagccgatac cttacagaat ttagcgacaa cctttaatat tccaccaaat
     7381 caatgggtgg cagtcggcga tggggcaaat gacttaccaa tgttaaaaac cgccagttta
     7441 ggcgttgctt tgcacgctaa accaaaagtg caagcacaag ctgattttgt ggtaaacttc
     7501 ggggacttaa cggcattatg tttattactt aatgccaatc atttatttca acattaattg
     7561 agaggaacaa caatgccatc atttgatatt gtatctgaaa tcacgatgca cgaagtgcgt
     7621 aatgcggtag aaaatgccaa tcgtgtatta agcacccgct acgatttccg tggggtggaa
     7681 gccgttattg agttaaatga aaaaaatgaa agcattaagc tcacgaccga atcggatttc
     7741 caattagagc agttaattga gattttaatc ggctcttgtg taaaacgtgg gatcgagcat
     7801 agttcattag atatccctac agaaagtgag catcacggta aactctattc caaagaaatt
     7861 aagctcaagc aagggattga gtcagatatg gcgaagaaaa ttaccaagct gattaaggat
     7921 tctaaaatca aggttcaagc ccaaattcaa ggcgagcaag tgcgtgtaac gggcaaatct
     7981 cgtgatgatt tacaagccgc aattcagcta gtgaaagggg cagatttagg gcagccattc
     8041 caatttaata atttccgtga ttagcaattc ctgcaattaa tgatgacaag tggaaaaatt
     8101 cagcaaaatt tgaccgcttg cattaataat tcaacataaa aggaagagat gatgagcgag
     8161 caaacagaaa agaaaggctg gtttaaaaaa gcccttgaga aatatgatgc cttttgcgat
     8221 gagctagggg taaatcaagg tgcttgccga ggttgtgtcc ctgtggtgaa gtttgaccct
     8281 gaaccggaaa aagagcagcc agaaaaatca gaaaataaaa catagaaaaa atgcacaatg
     8341 gtaagataca ttgtgcattt tttataggat ttttaattgg cgtgatgatg ggcaaaatat
     8401 tgcttaactc cgccatcttt cttgcaagga ttctgaatat cagtagagta actaccgtta
     8461 aaacaaagag cagtagcatt cgatggttta atcttttcct gtttagttag ggctggcggt
     8521 tgagtattgg tttcactaat atagatcgtt ttaggttgag aatagtctat atttttgtcg
     8581 ccatttacga cttgaacggg ggtgttcgag ctgcaagcta caaccgatgc tgaagccaat
     8641 gtaagtataa tgatatggtg ttttttcatg ggtaatttcc gtttttatat ttttaattga
     8701 gagaatgtta aaaagggctg atttctcaac ccttaaagtt atgccttaag catttttatt
     8761 aaaagtttgt cctaactgaa tttcatcatt taatacagga attaactgct ctagttgatc
     8821 tttttcaaat tcgctgagat tgccgatagg tagaactttt tccacaccat ttgtacctaa
     8881 acgaatcggg taagcaaaga atgccggaga ggtttcgcca ttggcattat ctacataagc
     8941 ataacgaatg acatcttccc cgattaaggc tctcactaaa ccaagtgcaa attctgctcc
     9001 tgcttgagcc atagataagg ttgctgagcc accacctgct tttgcctcaa ccacttcagt
     9061 accggcgttt tggatacgat gagttaattg ctcgatttgt tcttgagtga actcaagttt
     9121 taagccattt actgttgctt gtgaaagcag tggaagaata gttgtgccgg aatgtccgcc
     9181 aatcacaggc actcttacat atttcacatt tacgtctttc gcttcagcca caaaggtatt
     9241 cgctcgaata acatctaatg ttgtaacacc gaaaagttta tttttgtcat acacaccggc
     9301 ttttttcaat acttctgccg caatcggaat aatggtgttt accgggttag taataattgc
     9361 aatgcaagct ttagggcaaa ctttcgctgc tttttcgact aagttatgca cgatagtggc
     9421 attcgtattg aataaatcgg cacgtgtcat ccccggctta cgggcaacac cagcagaaat
     9481 aacgaccaca tcggcatctt ttagtgcctc agtcggatct tcacctgcaa aaccggttac
     9541 ttttacatca gttggaatat ggcttaaatc cactgcaatt cctggagtaa caggggaaat
     9601 atcgtaaaga gataattcag atttagccgg taaattgagt tttaataata aggcgagtga
     9661 ttgaccgatt ccacctgcag cacctaaaag ggcaactttt ttactttgac acataaaatt
     9721 tctcctaaat gttatggaag tttagaccaa tttagtctaa gccaattttt tgggcgattc
     9781 aaacggcaaa tctcattttt gagacataga gcaaattttt agctgatttt tatgggtttt
     9841 attgatttat ttgcataaat aagcaagaat tatgaatatt tttaagtttt tgtgaataag
     9901 agtgtgaaag tggacaagat agatagttta tcagaagcgt ttaaatcgct gttaaaggaa
     9961 gaaaaattcg gctcgcaaag tgaaattgtt gccgcactac aagagatggg ctttgataat
    10021 atcaaccaat ccaaagtttc tcgaatgtta tcaaaatttg gtgcagtcag aacccgtaat
    10081 cctaaaatgg aaatggtgta tcagctacca gtcgaattaa gtgtaccgac tacctccagc
    10141 ccacttaaaa atttggtggt agatattgat cataatgatg cgttaattgt ggtcaaaacc
    10201 agccccggtg cggctcaact gattgcacgt ttgctcgatt caatgggtaa agccgaaggt
    10261 attttaggca ctattgctgg cgatgatacg atttttataa cgccggtacg caatgtgcaa
    10321 attgaaaaat tggtgaagaa tgtgacacag ttatttgaaa catcattata gaataagtaa
    10381 aggaggactt aatgttctcc ttatttttta ggagattatt atgcacattt ttataacagg
    10441 tggtacgggt tttattgggc gtgaattaat ccagcaatta aaatctgaaa atcaccactt
    10501 tacacttctt actagagata cagaagcgaa aagtgatgat ttacagatta attattgcta
    10561 ttcacttgat gaacttgttg atttaaatga ttttgatgca gtaattaacc ttgccggtga
    10621 gccgattttt gataaacgtt ggacagacga acaaaaacag aaattgattg aaagccgggt
    10681 taacctaacg aataagctca gtcaattaat gaatgcaagc tctaaccctc cgcatacttt
    10741 tctctccggt tcggcaatcg ggatttatgg cgatttgcct ccttctgcaa atttttatac
    10801 agaatcgacc gcttgttatc aagattgctt taccaaccgg ctttgccaac agtgggaagc
    10861 cgctgcacta aaagccgcaa gtgataaaac ccgagtgtgt ttaattcgta ccggcacggt
    10921 attttcgccc gaaggcggag cattaaagaa aattttaccg ctttatcgat gcggattagg
    10981 cggaaaactt ggttcaggca aacaatattg ggcgtggatc agccgccggg atcacgtaca
    11041 agcggtcatt tttctgctca aaaatgcaaa ttgttcgggt gcttataatt ttgttgcacc
    11101 aaatccggtg agaaatgaag aatttaatcg tattttagcg aatgctgtta atcgcccggc
    11161 attctttact gtgcctgaat ttgccttgaa attaggctta ggcgaacgag cttgtttagt
    11221 cttagataat caacagctca ttccggaaaa actactcgca gccagttttg tattccaaga
    11281 tccgatttta accgagaacg ttttcttaaa cccataattt aggctataat gctgtataaa
    11341 agcacagatt aaaaggtgat tatggatttc tcattattat tagacggttt aaatgacaag
    11401 cagagggagg cggttgctgc cccgattggt aattatttag tgttagccgg ggcgggttcg
    11461 ggcaaaactc gggttttaac ccatcgcatt gcgtggctga ttggggtgga aaatgtgccg
    11521 gaatccaata ttctggctgt tacctttacc aataaagcgg cggcggaaat gcgacatcgg
    11581 attgagtaca ctctttccca atcgggcgat aatcgattgt tcggaatgtg ggttggtact
    11641 ttccacagca ttgccaaccg cttacttcgt tcccactatt tagatgcgga tttaccgcaa
    11701 gatttccaaa ttatggatac cgaagatcag caacgtttac tcaaacgttt actgaaactg
    11761 cataatattg atgagaaaca ttttccgcca aaacacgttg cgtggtatat caacgcccaa
    11821 aaagataaag gcaaacgggc aaaagatatt gatcactata acgatccgaa tgaaaaaaaa
    11881 ttggtggaga tttaccaaat ctatcaagat gcctgcgacc gagccggttt gttagatttt
    11941 gccgagctgt tgattcgggc ttatgaacta ttcaaaacca aatcaaccat tttgcaacgt
    12001 tatcaacaac gttttcagca aattttgatt gatgagtttc aagacaccaa caatattcag
    12061 tacgacttaa ttcgtttgct tgccggcgat gtcggcaaag tgatgattgt cggcgatgat
    12121 gatcagtcta tttatggttg gcgtggggca caggttgaga atatccaacg tttcttaaat
    12181 gattatgaca acgctcaaac cattcgctta gagcaaaatt accgctctac cggcaacatt
    12241 ttaagctgtg ccaatgaatt aattgccaat aacgataacc gcttaggcaa aaatttatgg
    12301 acagatagcg gtgatggcga gcctgtggat atttactgtg ccttcaacga acttgatgaa
    12361 gctcgctttg ttgcctcgca aatcaagcaa tggaaagaag acgagggtga tttggcggaa
    12421 tgtgcggtgt tgtatcgcag caacagccaa tctcgtgtga ttgaggaagc tttaattcaa
    12481 gcgaatattc cttatcgcat ttacggtgga atgcgattct ttgaacgcca agaaattaaa
    12541 gatgctctgg cttatttacg cttaatcgcc aatcgtcaag acgatgcggc gtttgaacgg
    12601 gtgatcaaca cgccaaccag aggcattggc gatcgtacct tagacacact ccgtcaaatt
    12661 acccgtggtc gccaaattac gctgtggcaa gcaattcaag tggcggtgca agaagaacaa
    12721 ctttcagggc gtgccacttc ttccttattg cgttttgtag agctgattaa tgctcttgat
    12781 caagaaaccg agcagatgtc gcttgccgaa caaaccgatt ttgtgattaa aaaatcgggt
    12841 ttatatgaaa tgtataagca agaaaaaggc gaaaagggcg aagttcggat tgaaaactta
    12901 gaagagttag ttaccgcaac taagcagttt gtgaaacctg acgaagcgga agagctgacc
    12961 gatcttaccg catttttaac ccacgcctca cttgaggctg gagaagccca agcctcgcaa
    13021 cacgaagatt atgtcgaact gatgacgcta cattccgcca aaggattgga gtttccccgt
    13081 gtgtttattg tgggcgtgga agaagggatt ttcccaagcg gaatgagctt tgatgaaggc
    13141 aggttgcaag aagaacgccg tttggcttat gtcggcataa cccgagctaa gaaaaaactg
    13201 acgattagct atgccgaatc aagacggctt tatggtaaag aagaacgcca cgttccgtct
    13261 cgctttattg ctgaattacc tgaagagcat attcgagaag tgagattgag aggctcaatt
    13321 aaccgagcgg caagttttaa ccaagctaat tcatttgcaa aaaatccgac aaaaacgacc
    13381 gcttcctttt taaacgatga tacttggaaa atggggcaga aagtgaacca cgctaaattt
    13441 ggacaaggca cgattattaa tgtggagggc tcaggtgaag ccacccgttt gcaagttgcc
    13501 tttgtcggca acggcattaa atggctgatt gccaaaatgg caaatttaga gaggatttag
    13561 gcgaataaaa agttttttgc aggaatatag atggcgagat atattgcccc ttaaccttaa
    13621 actaagggta aataaaat
//
LOCUS       DANLXF010000053        13609 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.053, whole genome shotgun sequence.
ACCESSION   DANLXF010000053 DANLXF010000000
VERSION     DANLXF010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13609
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.053"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            53..1006
                     /locus_tag="PXD66_000957"
     CDS             53..1006
                     /locus_tag="PXD66_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL6319277.1"
                     /translation="MEIILRLLWRRLMALPVMILGVTALVFIVLQFTPGDPATVALGE
                     SASEAAKEIYREQHGLNDPLLVQYFRFLGNLFVLDFGMTTPPEQPIVDMIAKAFPLTL
                     QLTFIGVFLAAIVSFTLGVTAALYRDTWIDQLIRLISVAAVATPSFWLGILLIQYFSL
                     KLDWLPSGGFIPFSEDPKGYVNSMVLPSLALAVPVCASLIRVVRTTMVEEMDKDYVRT
                     AIGNGVPYSTVIRHNVLRNALITPVTVLGLRVGYLLGGAVVIEQIFDLPGMGKLIFNG
                     IVNHDLNLVQGVVLTIAFTFVLVNIIVDILYLLINPKIRSL"
     gene            1008..2978
                     /locus_tag="PXD66_000958"
     CDS             1008..2978
                     /locus_tag="PXD66_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HDL6319278.1"
                     /translation="MFRQGLAARLASGGARFRALSTSSKIALLFIVFIALVAIFAPLV
                     APYDPLQTLRPVQAPSGDYLFGTDRLGRDIFSRMVWGARTSLFIGLGAVGVAILFGSI
                     LGATAATADKFGNEVIMRFMDILMAFPGIALAAVLLATFGNSVPVIIVTIAVVYTPQL
                     ARVVRANVVSQWEEDYVRAERVIGGSRTYILLKHVVRNTAAPVLVFATVMVADAIVFE
                     ASLSFLGAGVQPPFPSWGNILSEGRNLVLSGFWWATTFAGIMILLTVLALNILAEGLT
                     DALVNPKLKRSTQSKEDVAKPLATDVQEAMSETLALKRYLLKLHEKETTRTDRMQLNS
                     TAKPILQVKNLSIRFPNRYGETPLVDNISFTVHEGETMGLVGESGCGKSITAFSIMGL
                     LPKTAKITGEILFTDRSGKQHNLLESADLNALRGHEISMIYQDALSALNPSMRIKDQM
                     AQLISRGGKQSAETLLQWVKLDPEKTLNRYPHELSGGQRQRVLIAMALAREPKLLIAD
                     EPTTALDVTVQAEVIKLLNELREKLGFAMVFVSHDLALVAQIAHHITVMYAGQVVETA
                     PTTPLLANPTHEYTRGLLGSVLSTELRAERLYQIPGSVPSPFDFAKGDRFASRSLRPD
                     ANPEQHLKLVPTGDHPQHFWASHLEDPQSKEA"
     gene            2993..3799
                     /locus_tag="PXD66_000959"
     CDS             2993..3799
                     /locus_tag="PXD66_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL6319279.1"
                     /translation="MSMKFIKEQPIIELSNIVVQFASRDGTLFNPKKFTAVNDVSLAI
                     HAGETVGLVGQSGCGKSTLASVMIGLQKPTSGTVKFNGLEMKYGSAEARKYFGSQVSV
                     IFQDPATALNPRMRVLDILKDPMDIHNILQPQEREKRVYDLLSRVGLPRSAALVEPTR
                     LSGGQKQRVAIARALALNPKLIVADEPTSALDVSVRAQVLNLLADLKKELNLAMVFIS
                     HDLQTVHQVSDRIVVMNGGKIVEMGDASDVFERPTQEYTRTLIEAAPSLL"
     gene            3862..5448
                     /locus_tag="PXD66_000960"
     CDS             3862..5448
                     /locus_tag="PXD66_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HDL6319280.1"
                     /translation="MIQITQTINIDLLIVGSGIAGLSAAVEAKEKGINTLLISKSTIG
                     SGASYFPLKATLGIQVTGDEADKIHFKEDIENIGKGKINPNVVQAYIEDSPQAIELLH
                     RIGFQPWKRNDNRPACFAKYPRPIYLIKDWRESALRAKQILQEQHIAFYENATLLHIV
                     TDNNQVQGAVFSLTTSGQISYIFCKTKHLILAAGGIAGLYKDNLYPADVIGSLHYVAQ
                     QAGATLVNLAFIQFIPAFVEPKYKVLFGEHTLKYVTQITDSKGNNLFPHLNAEQFAKM
                     VLERSHYAPFSLDFAGVEFDLVMMKHLLENPHEKGIYLKYSPKLYEDTEEFYTVYLNW
                     LKNEVGIDLVQDKIAIKPFAHSCNGGIQINQWGESAVKGLFAIGEISHCIEGANRMGG
                     NSVGGGLVFAKRAVSEIVRSLEQNDLQKNSEISPLAMAEKALNQLANSTANLELTASE
                     VLSTIREKMALYANVYRTRSNLKLLLEELNRLEQEFDPIYHAQYQGIEIYNALKTAQL
                     VVNQMLNEQSLGAHYLDEQN"
     gene            5494..6876
                     /gene="nhaC"
                     /locus_tag="PXD66_000961"
     CDS             5494..6876
                     /gene="nhaC"
                     /locus_tag="PXD66_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL6319281.1"
                     /translation="MNNYLLKLKPMTAVILSLLTVFILGFTMIGLGWVPQISILAVIV
                     MLLLFGLINKIPFKNMQEGMASGLMSSIGAVYLFFFIGLLVSALMMSGAIPTLMYYGL
                     SVISPELFYISAFALSSVVGIALGSSLTTTATIGVAFIGMASAFGANPAIVAGAVVSG
                     AFFGDKTSPLSDTTTIAASVVGIDMFAHIRNMLYTTIPAWILTALFLWFLTEPTDAQN
                     LANIENLKTNLQETGLIHGYSLLPFVTLILMAIKRVNSIYIIVTTVTLSLIITYIHSS
                     PTLEQLGNYFFTSYKPVEGVDLGEVASLVSRGGINSMFFTITIVLLALSLGGLLQILG
                     ILPALLNGISGFLTSTGRVTLVVASTAVGINVLIGEQYLSLLLSGNTFRSTYERLGLE
                     VKNLSRTIEDAGTVINPLVPWGVCGVFISSVLNVPVLDYLPYAFFCYICLILTLIFGF
                     TGVTISKKAN"
     gene            complement(6919..8115)
                     /gene="ispC"
                     /locus_tag="PXD66_000962"
     CDS             complement(6919..8115)
                     /gene="ispC"
                     /locus_tag="PXD66_000962"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HDL6319282.1"
                     /translation="MKKLVILGSTGSIGKSTLSVVEHNPNPNQYEVFALVGGKNVELM
                     LEQCRQYQPTFAAMDDEKSAQALKTALATLGSKTEVLAGQAAICELAAHSEVDMVMAA
                     IVGAAGLLPTLSAVKAGKTVLLANKESLVTCGQIFIDEARKSGAKLLPVDSEHNAIFQ
                     SLPPEAQQKVGFCPLAELGVSKIILTGSGGPFRTKPLNEFDAITPAQAVAHPNWSMGK
                     KISVDSATMMNKGLEYIEARWLFNATAEEMDIIIHPQSIIHSMVRYIDGSVIAQMGNP
                     DMCTPIAHTMAYPNRINAGVPPLDFFKLKELTFIEPDFARYPNLKLAIEAFAEGQYAT
                     TAMNAANEIAVEAFLNEQIRFTDIVNVNRTVVENIAPIQVSEIADVLHIDKLARELAK
                     QAVLQL"
     gene            complement(8234..10432)
                     /gene="relA"
                     /locus_tag="PXD66_000963"
     CDS             complement(8234..10432)
                     /gene="relA"
                     /locus_tag="PXD66_000963"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HDL6319283.1"
                     /translation="MVAIRYSHQLEPNNFELSSWCASLNMSPVTFEQIHNAWSYAQEK
                     LDTDYSLMWYGIEMVEVLHGLNMDDDSLVAAMLFPLVKHNVIDLVQLKEDFSNDIKNL
                     VKGVLEMENIRQLSANNVSDLQIDNIRRMLLAMVDDFRCVVIKLAERIAYLRDSNHHS
                     EEDTVLAAKECSHVYAPLANRLGIGQLKWELEDYCFRILHPVSYTQIAKFELGERRVD
                     RERYIANFVADLTACLSPEIKNVQIYGRPKHIYSIWKKMQKKRLRFNQLFDIRAVRVI
                     VPTLEDCYTALGIIHTKYTHLPEHFDDYIANPKPNGYQSIHTVVLGEGEKTIEVQIRT
                     QQMHDDAELGVAAHWKYKEGATAGRSGYEEKIVWLRKLLEWQNDLADSGDMVAEMRSQ
                     IFDDRVYVFTPRGEVIDLPQKATPLDFAYAIHSEIGHRCIGAKVAGRIVPFTYHLQMG
                     DQVEIITQKEPNPSRDWLNPNTGFVNTGKARSKIIAWFKKLDREKNIPIGKEALEAEM
                     EKFGFTLKQIEEYALPRYNLKQFDDLYAGIGGGDIRLHQLMHFLQSKLIKPTAEQEDE
                     AVLKQVNKNTNQPKPSKGYVIVEGVGNLMHSIARCCQPIPGDEIVGYITQGRGISIHR
                     ADCEQLFELQSLNPERVVEADWGEGYTSGLSLTIRVIANDRNGLLRDVSAIMANEKVN
                     VLGVSSRTDVKRSLATIDMEIQLNNVEILNKLLARIAQLDDVIEAKRLSS"
     gene            10686..11993
                     /locus_tag="PXD66_000964"
     CDS             10686..11993
                     /locus_tag="PXD66_000964"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HDL6319284.1"
                     /translation="MTILALGINHKTASVGLREKVAFVDDKRKLALEQIQTSGLAESV
                     VILSTCNRTELYFHQPNISPREESEENIQWREQCFRWFAQIHQLDESELRECLYFKQN
                     LEAANHLMKVASGLDSLILGEPQILGQVKQAYQYSEDFYQHQQKAMSTKLSRLFQRTF
                     ATAKRVRSETEIGSQAVSVAYAACGLARQIFDNFGKLRFLLVGAGETIELVARYLIQH
                     GAKNIMLANRTHIRAEMLAEKLNAPMQILSLSALQLGLNQADVVISSTGSPDTLISKE
                     MVEIAQKQRQFDPLLLIDIAVPRDIDEKAGDLEGVYAYSVDDLQNIIQRNMAQREEAA
                     KQAAEIVKEESKAFFEWLKQQQSSDLIKRYRQDAEQTRQELLEKALQAISQGQDSQKV
                     LSELSYKLTNQLLHAPTQALQAMAKSGNSTGLQSFSRALRLEE"
     gene            12178..>13609
                     /gene="der"
                     /locus_tag="PXD66_000965"
     CDS             12178..>13609
                     /gene="der"
                     /locus_tag="PXD66_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDL6319285.1"
                     /translation="MTPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGH
                     ANIAGYDFIVIDTGGIDGTEEGVEEKMAEQSLLAIEEADVVLFLVDARAGLLPADVGI
                     AQYLRQREKTTVVVANKTDGIDADSHCAEFYQLGLGEVEQIAAAQGRGVTQLIEQVLA
                     PLGEQLNAEQFNQEQAGENAKNVANEETDEWDTEFDFENEEDAALLDEALAEEAEESI
                     EDKNIKIAIVGRPNVGKSTLTNRILGEERVVVYDMPGTTRDSIYIPMERDGQQYTIID
                     TAGVRKRGKVNLAVEKFSVIKTLQAIQDANVVLLTIEAREGISDQDLSLLGFILNAGR
                     SLVIVVNKWDGLSQDIKDQVKSELDRRLDFIDFARVHFISALHGSGVGNLFDSVKEAY
                     ACATQKTSTSMLTRILRMAADEHQPPLVNGRRVKLKYAHPGGYNPPIIVIHGNQVEKL
                     ADSYKRYLSNYFRKSLKIIGSPIRILF"
BASE COUNT         3870 a         2936 c         2806 g         3997 t
ORIGIN      
        1 taggtaaact tagatttaat tttggagtgt aactacactc aaggagaaga aaatggaaat
       61 tatactccga ttattatggc gtcgcctaat ggctctgccg gtaatgatct taggcgtaac
      121 tgccttggta tttattgttt tacaattcac ccccggagat ccggcaaccg ttgctctcgg
      181 tgaaagcgcc agcgaagccg ctaaagaaat ctatcgtgaa caacatgggt taaatgatcc
      241 attgcttgta caatattttc gctttttagg gaatttattc gtattagatt tcggtatgac
      301 aacccctcca gaacagccta ttgttgatat gattgctaaa gcattccctt taacattaca
      361 gcttaccttt attggggtct tcttagctgc tatcgtctct ttcacattag gtgtaacggc
      421 agcactttac cgtgatactt ggattgatca attaatccgt ttaatctcgg ttgccgctgt
      481 tgccacacct tctttctggt tgggtatttt attgattcaa tacttctcct taaaattaga
      541 ttggttgcct tcaggcggtt ttattccatt tagcgaagat ccaaaaggct atgtaaactc
      601 aatggttctc ccttcactcg ctttagctgt tcctgtatgt gcctcattga tccgtgtcgt
      661 gcgtacaaca atggtagaag aaatggataa agattatgtt cgaacagcca tcggtaatgg
      721 tgtaccttac tcaacggtga ttcgccacaa cgtgcttcgt aatgccttaa ttactccggt
      781 aacagtatta ggtttacggg ttggttattt attaggtggt gcagttgtga ttgaacagat
      841 cttcgactta ccgggaatgg gtaaattaat tttcaacggt atcgtcaatc acgacttgaa
      901 cttagtgcaa ggtgtagtat taactatcgc atttactttc gtgttggtga atatcattgt
      961 tgatattctt tacttgctca ttaatccaaa aattcggagt ttataatatg tttcgccaag
     1021 gattagctgc tcgacttgct tcaggcggtg caagatttag agcattatca accagctcaa
     1081 aaatcgcctt actctttatt gtatttatcg cattagtcgc tatttttgca ccattagtag
     1141 cgccttatga cccactgcaa acgctacgtc cagtgcaagc tccaagcggt gattatttat
     1201 tcggtacaga ccgtttagga cgtgatattt tctcgcgtat ggtatgggga gcaagaacat
     1261 ctctctttat cggtttaggt gcggtaggcg ttgcgattct attcggtagt attttagggg
     1321 caactgcggc aaccgccgat aaattcggta acgaagtgat tatgcgtttt atggatatct
     1381 taatggcatt cccgggtatc gcattagctg ctgtattact tgccactttc ggtaactctg
     1441 tacctgtgat tatcgtgact attgcggttg tttacactcc ccaacttgcc cgagtggtgc
     1501 gtgctaacgt agtatctcaa tgggaagaag attatgtgcg tgccgagcgt gtaattggtg
     1561 gtagccgtac ttatattcta ttaaaacacg ttgtgcgtaa tactgctgca ccggttttag
     1621 tattcgcaac agtgatggtt gctgatgcca tcgtatttga agcatcactt tcattcttag
     1681 gtgcaggtgt ccagcctcca ttcccatctt ggggaaatat cttatctgaa ggtcgtaacc
     1741 ttgtattaag tggcttctgg tgggctacaa ctttcgcagg gattatgatt ttattaaccg
     1801 tacttgcgtt aaatatcctt gctgaaggct taaccgatgc gttagtaaat ccaaaactaa
     1861 aacgttcaac tcaaagtaaa gaagatgtcg caaaaccatt ggcaacagac gttcaagaag
     1921 caatgtctga aactcttgcg ttaaaacgtt acttactcaa attacacgag aaagaaacga
     1981 cccgtactga ccgtatgcaa ttaaactcaa cggccaaacc gattttacaa gtgaaaaact
     2041 tatcaatccg tttcccaaat cgttatggtg aaactccgct tgttgataat atcagcttta
     2101 ctgttcacga aggcgaaacc atgggcttag tgggtgaatc tggttgtggt aaatcaatca
     2161 ccgcattctc aattatgggt ttattaccaa aaactgcaaa aatcactggt gaaatcctct
     2221 ttaccgaccg ttcaggtaaa caacacaatc ttttagaatc agccgattta aatgcccttc
     2281 gtggtcacga aatctcgatg atttaccaag atgcattaag tgcgttaaac ccatcaatgc
     2341 gtattaaaga ccaaatggca caattaatca gccgtggtgg taaacaatca gcagaaacat
     2401 tattgcaatg ggtaaaactt gacccggaaa aaacgctcaa ccgttatcct cacgagttat
     2461 ctggaggaca acgtcagcgt gtattaattg caatggcact tgctcgtgaa ccgaaactat
     2521 tgattgctga tgaaccaaca acagcattag acgtaaccgt tcaagctgaa gtgatcaaat
     2581 tattaaacga attacgtgag aaacttggat ttgcaatggt attcgtgagc cacgacttag
     2641 ctttagtggc acaaatcgct caccacatca cggtaatgta tgctggtcaa gtggttgaaa
     2701 ctgcaccaac tacgccatta ttggcaaacc caacacacga atacacacgt ggattattag
     2761 gctcggtact ttctaccgaa ttgcgtgcag aacgcctata ccaaattccg ggcagtgtgc
     2821 cttcaccatt tgattttgcg aaaggtgacc gttttgcgag tcgttcatta agaccagatg
     2881 caaaccctga acagcacttg aaattagtgc caaccggtga tcacccacaa cacttctggg
     2941 cttcacactt agaagatcca caatctaaag aagcataaag gagagcaata cgatgagtat
     3001 gaaatttatc aaagaacagc caattattga actgtctaat attgttgtgc aatttgcttc
     3061 tcgtgacggc accttattta acccgaaaaa attcacggcg gtaaatgatg tcagcctagc
     3121 aattcacgcc ggtgaaacag tcggtctggt tggacaatct ggttgtggca aatctacttt
     3181 agccagtgtg atgatcgggc ttcaaaaacc aacctccggc acagtaaaat ttaacggctt
     3241 agagatgaaa tatggcagtg cggaagctcg caaatatttc ggtagccaag tgtcagtaat
     3301 tttccaagac ccggcaaccg ccctaaatcc gcgtatgcgt gtgttggata ttttaaaaga
     3361 tccgatggat attcataata tcttacaacc gcaagaacgt gagaagcggg tttatgattt
     3421 gctctctcga gtcggtctac ctcgttcagc ggcgttggtc gagccaaccc gattatctgg
     3481 tgggcaaaag caacgtgtag cgattgctcg tgcattggca ttaaatccga agctgattgt
     3541 ggctgatgaa ccaacctctg cacttgacgt atcggttcga gctcaagtac tgaacctgct
     3601 ggcagacttg aaaaaagaac tcaatttagc gatggtattt atctctcacg acttacaaac
     3661 cgttcaccaa gtttcagacc gcattgtggt tatgaacggc ggtaaaattg tggaaatggg
     3721 cgatgcttca gacgtatttg aacgcccaac gcaagagtac acccgaacct taattgaagc
     3781 agcaccatct ttattataat tgtgctttgt gcctacaagc ggtaaaattt ttcgcaaatt
     3841 ttaccgcttg tgtttttatt tatgatccaa attacccaaa ccattaacat tgatttatta
     3901 attgtcggct ctggcatcgc tgggctttct gctgcagttg aggcaaaaga aaaaggcatt
     3961 aataccttac tgattagcaa atctacgatt ggctcgggag cgagctactt tccgcttaag
     4021 gcaacacttg gcattcaagt aaccggcgat gaagccgata aaattcactt taaagaagat
     4081 attgaaaaca tcggcaaagg caaaatcaac cctaatgtgg tacaagccta tatcgaagat
     4141 tcaccacaag cgattgaact actccaccgt atcggctttc agccttggaa gcgtaacgac
     4201 aaccgtccgg cttgttttgc caaataccct cgcccaattt atttaattaa agattggcgg
     4261 gaatctgccc tacgagcgaa acagattttg caagagcaac acattgcttt ctatgaaaat
     4321 gctacccttt tacacatcgt cacagacaat aaccaagtgc aaggtgcggt attttcgctc
     4381 actacaagcg gtcaaatttc ctatattttt tgcaaaacca agcatcttat tttagccgca
     4441 ggcgggattg ctgggctgta taaagataat ctctacccgg ctgatgttat cggttcactt
     4501 cattatgtgg cacaacaagc cggggcaacc ttggttaatc ttgcctttat tcagttcatt
     4561 cctgcctttg tcgagccaaa atacaaggtg ctatttggcg aacatacatt gaaatatgtc
     4621 acccaaatca ccgattctaa gggtaacaat ctatttccac acttaaatgc cgagcaattt
     4681 gcaaaaatgg tgctggaacg tagccattat gctcctttca gccttgattt tgccggcgtg
     4741 gagttcgatt tagtgatgat gaaacatttg cttgaaaacc ctcacgaaaa aggcatttac
     4801 cttaaataca gccccaaact gtatgaagac acggaagagt tttataccgt ctatttgaat
     4861 tggctcaaaa atgaagtcgg gattgattta gtgcaagata agattgcaat caagccgttt
     4921 gctcatagct gtaacggcgg cattcaaatt aatcaatggg gcgaaagtgc agtaaaaggg
     4981 ctatttgcga ttggtgagat ttctcactgc attgaagggg caaaccgtat gggtggtaac
     5041 tcagtcggcg gtggcttagt gtttgccaaa cgtgcggtat cggaaattgt tcgttcgcta
     5101 gaacagaatg atttgcaaaa aaacagcgaa atctcaccgc ttgcaatggc agaaaaagca
     5161 ttaaatcagc ttgctaattc aactgctaat ctagaactaa ctgccagtga agtgctttct
     5221 actattcgag agaaaatggc gttatacgcc aatgtgtacc gcactcggag caacctcaaa
     5281 ctactacttg aggaattaaa ccgtttggaa caagaatttg acccaatcta tcacgctcaa
     5341 tatcaaggca ttgaaattta caatgcgtta aaaaccgctc aattagtggt caatcaaatg
     5401 ctaaatgagc agagtttagg cgcgcactac ttagatgaac aaaattagtc tataatagcc
     5461 aacttattta aaaataataa attaggagta taaatgaata actacctact taaattaaaa
     5521 ccaatgacag cggtcatact ttcgcttttg acggtcttta tcttaggttt tacaatgatt
     5581 gggcttggct gggtgcctca aatttcaatt ctagccgtga ttgtaatgct gctacttttc
     5641 ggcttaatca ataaaattcc gtttaaaaat atgcaagagg gtatggcaag cgggctgatg
     5701 tccagtattg gtgcggttta cctattcttt tttattggat tattggtttc agctttgatg
     5761 atgtctggtg cgattccaac cttaatgtat tatggcttaa gtgtgatttc acctgaatta
     5821 ttttacattt ctgcattcgc attaagttct gtagtaggta ttgcgttagg cagtagttta
     5881 accacaaccg ccaccatcgg tgttgctttt atcggtatgg cttctgcttt cggtgcgaac
     5941 cctgcgattg tagcaggtgc ggtggtttca ggggcgtttt ttggcgataa aacctctcca
     6001 ctttcagaca ctactaccat tgctgcttcc gtagtgggca ttgatatgtt cgctcatatc
     6061 cgtaatatgc tttataccac catccctgcg tggattttaa cagcattatt cctttggttt
     6121 ttaaccgaac ctactgatgc acaaaattta gcaaatatag agaacctaaa aaccaatttg
     6181 caagaaacag gattaataca cggctactct ttgctacctt ttgttacgtt aattttaatg
     6241 gcaattaagc gtgttaattc aatttatatt attgtaacta cagtaacact ctcgctaatt
     6301 attacctata tccacagttc gccaaccctt gaacagctag ggaattattt ctttaccagc
     6361 tataaacctg ttgaaggcgt agatttaggc gaagtggcat cattagtctc tcgtggtggc
     6421 attaatagta tgttctttac catcactatt gtattgcttg cgttaagttt aggtggtctt
     6481 ttacaaatcc tcggcatttt accggcacta cttaacggca ttagcggctt tttaaccagc
     6541 acaggacgtg ttactcttgt agttgccagc actgcggtgg gaattaacgt cttaatcggt
     6601 gagcaatatt tgagcttatt gttatcaggt aatactttcc gctcaactta tgaacgttta
     6661 ggcttagaag tgaaaaactt atctcgcacc attgaagatg caggcacggt aatcaaccca
     6721 ttagtccctt ggggcgtgtg cggtgtgttt atcagtagcg tattaaatgt accggtgtta
     6781 gattatctac catacgcatt cttctgttat atctgtttaa tccttacctt aatttttgga
     6841 tttaccgggg taacaatcag taaaaaagct aactaatatg tgaaaaatgc ctacaaattt
     6901 gtaggcattt tcttttcatt agagctgaag aacagcttgt ttggctaact ctctggcgag
     6961 tttatcaata tgcaatacat cagcaatttc actgacttga attggagcaa tattttccac
     7021 caccgttcta ttcacgttca caatatccgt aaagcggatt tgctcgttta aaaacgcctc
     7081 aacggcaatt tcattggcgg cattcatcgc tgttgtcgca tactgtcctt ctgcaaaagc
     7141 ctcaatcgct aattttaagt tcgggtaacg ggcaaaatcc ggctcgataa aggttagctc
     7201 ttttaattta aagaaatcta atggtggcac accagcatta attcggtttg gatatgccat
     7261 cgtgtgagca atcggcgtac acatatccgg gttgcccatt tgagcaatca ccgagccatc
     7321 aatataacgc accatcgaat gaataattga ttgtgggtga atgatgatat ccatttcctc
     7381 ggctgttgca ttaaacaacc agcgggcttc aatatactcc aagcctttgt tcatcattgt
     7441 ggctgaatcg actgaaattt tcttacccat agaccaattg gggtgagcaa ctgcttgagc
     7501 cggtgtaatc gcatcaaatt catttaatgg cttagtacgg aacggtccac ccgatcctgt
     7561 taaaataatt ttactgacac ccagttcagc gagtggacaa aacccgactt tttgctgtgc
     7621 ctccggtgga agcgattgga aaatcgcatt gtgttcacta tctaccggta agagcttcgc
     7681 accggattta cgagcctcat caataaaaat ttgcccacag gttaccagcg attctttatt
     7741 tgccaacaat acggttttac ccgctttcac cgcagaaagt gtcggtaata accctgctgc
     7801 tcctacaata gcagccatta ccatatccac ttcggaatgt gcagcaagct cacaaatagc
     7861 tgcttgaccg gctaacactt cagttttaga ccccaaagtt gccaatgccg tttttaatgc
     7921 ttgtgcggat ttttcatcat ccattgccgc aaaagtcggc tgatattgac gacattgctc
     7981 cagcattaat tcaacattct ttccaccaac taacgcaaac acctcatatt ggtttgggtt
     8041 tgggttatgc tcaactaccg ataaagtgct tttaccgata gaacctgttg aacctaaaat
     8101 cactaatttt ttcattttgt ttctctttat ctaatgactg ataagggcga aagatttttc
     8161 gcccctacgc cgtaattatg cggatactca ttcgttgttt gtaaattgtt tagcaaaaac
     8221 aaccgcttgt agattaactc gaaagccgtt ttgcttcgat aacatcatct aattgagcga
     8281 tacgagccaa taatttattc aaaatttcaa cgttgttaag ctgaatttcc atatcaatgg
     8341 tggcaaggct acgtttcaca tcggttcggc tggaaacgcc taacacgttt actttttcat
     8401 ttgccataat tgcactgaca tctcgcagta agccgttgcg gtcattggca atcactcgaa
     8461 tggttaagct taaaccagag gtatagcctt cgccccaatc cgcctccaca actcgttctg
     8521 ggttgagact ttgtaattca aacagttgct cacaatctgc acggtgaatc gaaatccctc
     8581 tgccttgtgt gatgtaaccc acgatttcat cacccggaat cggctggcag caacgggcaa
     8641 tggaatgcat taagttccct acgccctcta caatcacata gcctttgctt ggcttcggct
     8701 gattggtgtt tttattcacc tgtttaagta ccgcttcgtc ttcttgttcg gcggtcggtt
     8761 taattaattt tgattgcaag aagtgcatta actgatgaag acgaatatcg ccaccgccaa
     8821 taccggcata gagatcatca aactgtttta agttatagcg aggcagagca tattcttcaa
     8881 tttgcttgag cgtaaaaccg aatttttcca tttcggcttc aagggcctct ttgccgattg
     8941 gaatattttt ctctcggtct aattttttaa accacgcaat aatttttgag cgagctttgc
     9001 ctgtattcac aaaaccggta ttcggattga gccaatctcg gcttgggttc ggctcttttt
     9061 gggtgataat ttcaacctga tcgcccattt gcagatgata agtaaacggc acaatccgcc
     9121 ctgccacttt tgctccaatg caacgatgcc cgatttcact atgaattgca taagcaaaat
     9181 caagtggtgt agctttttgc ggtaaatcga tcacctcgcc tcttggggta aagacataaa
     9241 cacgatcatc aaaaatttgc gaacgcattt ctgccaccat atcgcctgaa tcggcaagat
     9301 cgttttgcca ctcaagcaat ttgcgtagcc acacgatttt ttcttcgtag cccgaacgcc
     9361 ctgcggttgc cccctcttta tatttccagt gagcggctac gcctagctcc gcatcatcgt
     9421 gcatttgctg ggtacgaatt tgcacttcaa tcgttttttc accttcgcct aatacaactg
     9481 tatgaataga ctgataaccg ttaggttttg ggttagcaat ataatcatcg aaatgttccg
     9541 gcaaatgggt atatttggtg tgaataatgc ctaatgccgt gtagcaatct tctaacgttg
     9601 gcactattac ccgcacagcc cgaatatcga aaagctgatt aaatctcaac cgttttttct
     9661 gcattttctt ccaaatgctg tagatatgtt tcgggcgtcc ataaatctgc acattcttga
     9721 tttcaggact taaacaagcg gtcagatctg ctacaaaatt tgcaatataa cgctcacgat
     9781 ccacacggcg ttcgcccaat tcaaatttag caatttgagt gtaagagaca gggtgtaaaa
     9841 tgcggaaaca gtaatcttcc agctcccatt tgagctgccc gatacccaat cgattggcaa
     9901 gcggtgcata aacgtgagaa cattctttag cggcgagaac ggtatcttct tcggaatggt
     9961 ggttactatc acgcaggtag gcaattcgtt cggcaagttt aatcaccaca cagcggaaat
    10021 cgtccaccat cgccaacaac atgcgacgga tattgtcgat ttgcagatcc gacacattat
    10081 tggcactaag ctggcggata ttctccattt cgagtacgcc tttaactaag tttttgatgt
    10141 cgttactgaa atcttctttt aattgcacca agtcgatcac attgtgtttt accagaggaa
    10201 aaagcattgc ggcaactaag ctgtcatcgt ccatattcaa gccgtgcaat acttcaacca
    10261 tctcaatgcc ataccacatc agactatagt cggtatccag tttttcctgt gcataactcc
    10321 acgcattgtg aatctgctca aaagtcacag gagacatatt caggcttgca caccaactcg
    10381 acaactcaaa attattcggt tcaagttgat gggaataacg aattgctacc ataaggcctc
    10441 cttgtaaatt tttgtggaaa ttagaccgct tgtacagctt aaaatggagt tttattatac
    10501 gtggaaattc agaaaaaatt tacccctaat cgaaaattta atccaagtta tcttatcctc
    10561 tttactcctt aagaggtagc aaccttgtga taaatcaaac ctatcaacac aatcatttgc
    10621 acgacttcac gctcttgatt aaaatacttg cgtttttatt ttgatcttta tttttgtggg
    10681 tagcaatgac aattttagct ttaggaatca accataaaac cgcctctgtg ggtttaagag
    10741 agaaagtggc gtttgtagat gacaaacgta aactggctct tgaacaaatc caaacgagcg
    10801 gattggctga aagcgtggtc attctctcta cctgcaaccg caccgagctc tacttccacc
    10861 agcctaatat ttctccccgt gaagagagcg aggaaaatat ccaatggcgt gaacaatgtt
    10921 tccgctggtt tgcccaaatt catcaacttg atgaaagcga gttgcgtgaa tgtttatatt
    10981 ttaaacaaaa tttagaagcc gctaaccact taatgaaagt ggcaagcgga ttagattcac
    11041 taattttagg cgaaccacaa attttagggc aggttaaaca agcctatcaa tatagcgaag
    11101 atttctacca acatcagcaa aaagcgatgt ccaccaagct atcacgcttg ttccaacgca
    11161 cctttgccac cgcaaaacga gtacgttccg aaaccgaaat cggcagccaa gcggtgtcag
    11221 ttgcttatgc agcttgcggt ttagcccgtc aaattttcga taattttggc aagttacgct
    11281 ttttgctggt aggggcaggc gaaaccattg aattagtcgc ccgctattta attcaacacg
    11341 gggcgaaaaa tataatgttg gcaaaccgca cccatattcg ggcggaaatg ttggcggaaa
    11401 aactaaacgc tccaatgcaa attctgtcgt taagtgcttt gcaactcggt ttaaaccaag
    11461 ctgatgtagt tattagctcc accggtagcc ctgataccct aatcagtaaa gaaatggtgg
    11521 aaattgcgca aaaacaacgc caatttgacc cacttttgct gattgatatt gccgttcctc
    11581 gtgatattga cgaaaaagcc ggcgacttag agggcgttta cgcttatagt gttgatgatt
    11641 tacaaaatat tatccaacgc aatatggctc agcgtgaaga agccgccaaa caagccgctg
    11701 aaatcgtcaa agaggaaagc aaagcatttt ttgaatggtt aaaacaacag cagtctagtg
    11761 atttaatcaa acgctatcgt caagatgcgg agcaaacccg ccaagaattg ttggagaaag
    11821 ccctacaagc aatcagtcaa gggcaagaca gccaaaaagt attaagtgag ctaagctaca
    11881 aactcaccaa ccagttacta cacgccccaa cccaagcgtt acaagcaatg gcaaaaagtg
    11941 gcaactccac cggcttacaa agtttttcaa gagcattacg gctagaagaa tgatgctcag
    12001 cacattcttt taggcatatt tgcaaaaaat tgaacatttt taaccgcttg cggacgcacg
    12061 ccgtgcgtcc ctccccgtgt tcccgatctt cttctaattt caacttcccc aaatttccgc
    12121 tataataagc caatttttgc aactattttg caaataaccg gatatagagg atacaaaatg
    12181 actcctgttg ttgccctagt gggcagacct aatgtcggca aatcgacttt atttaaccgt
    12241 ttaacccgta ctcgagatgc cttagtagca gatttccccg gcttaacccg agaccgtaaa
    12301 tatggtcacg ctaatattgc cggctacgat ttcattgtaa tcgacaccgg tggtattgat
    12361 ggtacagagg aaggcgtgga agaaaaaatg gcggaacaat ctttattggc gattgaagaa
    12421 gccgatgtgg tgcttttctt ggttgatgcc cgagccggat tattacctgc cgatgtcggc
    12481 attgctcaat atttacgcca acgtgaaaaa accacggtgg tggttgccaa taaaaccgat
    12541 ggtattgatg ccgactctca ctgtgccgaa ttctatcaat taggcttagg cgaagtagaa
    12601 caaattgctg cagcacaagg gcgtggggtt acgcagctta tcgagcaagt gttagctcct
    12661 ttaggcgaac agctaaatgc cgagcaattt aaccaagaac aagcgggcga aaatgctaaa
    12721 aatgttgcaa atgaagaaac agacgaatgg gataccgaat tcgactttga gaacgaagaa
    12781 gatgcggcat tattagatga agccttggca gaagaagcag aagagtcgat tgaagataaa
    12841 aatatcaaaa ttgcgattgt cggtcgtccg aatgttggta aatcaacact gaccaaccgc
    12901 attttaggcg aagagcgtgt ggtagtttat gatatgccag gaacaacccg tgattctatt
    12961 tatattccaa tggagcgtga cggtcagcaa tataccatca ttgataccgc cggtgtgcgt
    13021 aaacgtggca aagtgaattt agcggttgag aaattctcgg taattaaaac cttgcaagcc
    13081 attcaagatg caaacgtggt gctattaact attgaggccc gagaaggtat ttccgatcag
    13141 gatttatcac tactcggctt tattctaaat gctggtcgtt cattggtgat tgtggtaaac
    13201 aaatgggacg gcttgtcgca agatattaaa gatcaagtga aatcagagtt agatcgccgt
    13261 ttagatttta ttgattttgc cagagtgcat tttatctcgg cattgcacgg ctcaggcgtg
    13321 gggaatttgt ttgattcagt aaaagaagcc tacgcttgtg caacgcaaaa aacctcgacc
    13381 tcaatgctga ctcgcatttt gcgtatggct gctgatgaac accaaccacc attggttaat
    13441 ggtcgccgag tgaaattaaa atatgctcac ccgggtggct acaacccacc aattattgtg
    13501 attcacggca atcaggttga aaaattagcg gattcttaca aacgttattt aagtaattat
    13561 ttccgtaaaa gtttaaaaat tatcggctcg ccaattcgga ttttattcc
//
LOCUS       DANLXF010000054        13539 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.054, whole genome shotgun sequence.
ACCESSION   DANLXF010000054 DANLXF010000000
VERSION     DANLXF010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13539
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.054"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(25..1564)
                     /locus_tag="PXD66_000966"
     rRNA            complement(25..1564)
                     /locus_tag="PXD66_000966"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(1767..1843)
                     /locus_tag="PXD66_000967"
     tRNA            complement(1767..1843)
                     /locus_tag="PXD66_000967"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1807..1809),aa:Pro,seq:tgg)
     gene            complement(1868..1943)
                     /locus_tag="PXD66_000968"
     tRNA            complement(1868..1943)
                     /locus_tag="PXD66_000968"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1908..1910),aa:His,seq:gtg)
     gene            complement(1973..2049)
                     /locus_tag="PXD66_000969"
     tRNA            complement(1973..2049)
                     /locus_tag="PXD66_000969"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2013..2015),aa:Arg,seq:ccg)
     gene            complement(2208..2930)
                     /gene="rffM"
                     /locus_tag="PXD66_000970"
     CDS             complement(2208..2930)
                     /gene="rffM"
                     /locus_tag="PXD66_000970"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HDL6319286.1"
                     /translation="MFDKVIIRGIALQAVKNREIFANWLMNEKRIQFGRLVAINAEKV
                     ILSEKNPLLRRVIDEAEFNYADGISVVQSIRKKYPNYKIERIAGADLWETLMQRAGAL
                     NVPVFLVGSSADTLTKVEAKLAQWKVNIVGRQNGYFKAEQEQDVIEQIKASGAKFISV
                     AMGTPKQELFIQQLQQQYPQALYMGVGGTYDVFAGKVKRAPKIWQNLGLEWLYRLLKQ
                     PTRWQRQLNLLKYAYYYLTNQL"
     gene            complement(2920..4191)
                     /locus_tag="PXD66_000971"
     CDS             complement(2920..4191)
                     /locus_tag="PXD66_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WzyE family oligosaccharide polymerase"
                     /protein_id="HDL6319287.1"
                     /translation="MNNLIPLGGFYLVSVGLFFSLLYQLYQKSGISFHLLFSLLYFVT
                     FYAGFPFSLIMAFGFDYPLPELKTQWQVFTAAGVGYLIYYVVYSRLWSAVSQPTALVA
                     NFQAKTTACILAAVAVVALLYFLYLNGFLLFKLEKYSQLFAKDRVILSALKRFFYFLL
                     PALLIFCFLYKNKTAWWALLIVGGAFGILSYVAVGGTWANLALAVGFFLLIGLQQGYL
                     SLSKVIAVSLIGVVAMFFLALARYKLTLSGSEMIYTFLYLSRDTFSPWENLARVFSNE
                     VEYQGLMPIVRDFYVYIPQSLWQERPEIVWNTANYFTKVILGNQSGLAISPTLLGSFY
                     IMGGFSVMALGMGIVAAMIKALDNLFAYAKACNSALLQAYCFGNLFNLIVLVREGIDA
                     FVSRFCFFTLIFMACYLLAKILSMGKNNNVR"
     gene            complement(4184..5254)
                     /locus_tag="PXD66_000972"
     CDS             complement(4184..5254)
                     /locus_tag="PXD66_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HDL6319288.1"
                     /translation="MLPIYHILGSEIEHHNQQVLTFFQQELLPLLPDSEHFFYVVGKP
                     SLQTAFPHLRIKQFSSQKAIANALIAKKRQNPTACFLLHGQFNPWIWLAIALGRLPTT
                     NLIWHIWGADLYEMATSWKFKLFYPFRRVAQQKILQVWATRGDLDHLWRNMRTRSEQD
                     HLIYFPTKLGDQIKISPTQNDRLTILLGNSGDPANELIQALSEIHQRLGNNVRIIIPM
                     GYPENNESYIRQVEAYSKRLFLAENLQILRKKLSFEEYVTLLSHCDLGYFNFKRQQGI
                     GTISLLISHNVPCVLHSANPFGLDLQAEKVPYLLAENLTASQIQTTKQQLKSIDKNRI
                     AFFAPGYTKLWQQRLIELMQNE"
     gene            5441..6862
                     /gene="glnA"
                     /locus_tag="PXD66_000973"
     CDS             5441..6862
                     /gene="glnA"
                     /locus_tag="PXD66_000973"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HDL6319289.1"
                     /translation="MSNAIANVSKLIQDNDIKFVLLKFTDIKGKEHGVSLPVSLVAND
                     LEDLFEEGKMFDGSSVEGWKAINKADMLLMPIAETAVVDPFARIPTLSIRCSIYDPNT
                     MQSYDRDPRSIATRAENYLKSTGIADSVMFGPEPEFFLFDDVRFSTEMNNVSYRIDDV
                     EAAWNTNRKFEEGNKAYRPLKKGGYCAVAPIDTAHDIRSEMCLLMEEMGLVIEAHHHE
                     VATAGQNEIATRFNSLTLKADETQIYKYIVQNVAVEHGKTACFMPKPFAGDNGSGMHC
                     NMSLSKDGKNVFMGDKYAGLSETALYYIGGIIKHAKALNAFTNPTTNSYKRLVPGFEA
                     PVLLAYSASNRSASIRIPAVTSPKATRVEARFPDPMANPYLCFAALLMAGLDGVINKI
                     HPGDAMDKNLYDLPPEELKDIPTVASSLESALDALSNDFEFLTQGGVFTKDFIDAYIG
                     IRRKEVERVNMTPHPVEFELYYS"
     gene            complement(6906..8049)
                     /gene="rffA"
                     /locus_tag="PXD66_000974"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /pseudo
     CDS             complement(6906..8049)
                     /gene="rffA"
                     /locus_tag="PXD66_000974"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251256.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
     gene            complement(8036..8680)
                     /gene="rffC"
                     /locus_tag="PXD66_000975"
                     /gene_synonym="wecD"
     CDS             complement(8036..8680)
                     /gene="rffC"
                     /locus_tag="PXD66_000975"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HDL6319290.1"
                     /translation="MKPDQWLSDFFGREIVQVKVAADDFRQIARLQKQGFVLVEGELE
                     FELALATFSENMTACRPADMADLPDLNLLFGSAFPDSRFRSPYFSREENQRFYRTWIE
                     NAVKGKFDDIGLVQCNADGQIQGAVTVRLLAQQQAKIGLLAVKPDCRQQGIGSELLNA
                     AIAWAKQQNAKKLMITTQLSNQKAIRFYQAFGASIKAVYYWFYPALEKYDDEQS"
     gene            complement(8677..9924)
                     /gene="wecC"
                     /locus_tag="PXD66_000976"
     CDS             complement(8677..9924)
                     /gene="wecC"
                     /locus_tag="PXD66_000976"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HDL6319291.1"
                     /translation="MMSKTEFNTICILGLGYIGLPTSVVFANAGKQVIGVDINPQVVA
                     SLNQGNIHIIEPDLQTAFTQAVKKGNFFATAKPQPADAFIIAVPTPLCGKQPDLTYIQ
                     NAAAMIAPCLRKGNLVVLESTSPVGTTENLAEWLSQARPDLAFPADVSIAYCPERVLP
                     GNIMTELVENDRIIGGLSEQCGQQAVDLYQIFAKGRCVTTTARTAEMCKLVENSFRDV
                     NIAFANEISMICDALKINVWELIELANLHPRVNILQPGAGVGGHCLAVDPWFIVSTSP
                     QQARLIRTAREVNDSKPQWVIEQVKTSVADCAMEQDCKPSELTIACLGLAFKPNIDDL
                     RESPALAITRQLAAWHQGSLLAVEPNIQQLAGEKITLVDLNTALSQADILVLLVDHTS
                     FKQISREQIRQRHIIDCRGVWVR"
     gene            complement(9965..10777)
                     /locus_tag="PXD66_000977"
     CDS             complement(9965..10777)
                     /locus_tag="PXD66_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS chain length-determining protein"
                     /protein_id="HDL6319292.1"
                     /translation="MKTLFYRLLVLILFAAIGAGAGYGLSYTQPEKWKVTAQFEQPTI
                     PDLGNYYSLLSTYHFLNSNSQEANSQTAQQQASEQVYAEFKRNLTSADLLQAYLLQTE
                     AVKLKAQVAKKSPAVVAAELVEQFRFEKDMATSADNVSLIAENAEEAQKLLADFIPLV
                     NTQARDKLNADLIAKWKVLFQQIKTASELNINGKQGSQDWAAKLQMMRSVQPLDNQLT
                     AYRLVKSPSVPLKSESPDPPILADDWGIKWLTAGLYAQFCGESESSTCGTLI"
     gene            complement(10788..11852)
                     /gene="wecA"
                     /locus_tag="PXD66_000978"
     CDS             complement(10788..11852)
                     /gene="wecA"
                     /locus_tag="PXD66_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HDL6319293.1"
                     /translation="MWLTFLTVFIVSFLALIVMRPVAEFVGLVDKPNFRKRHQGLIPL
                     IGGIALFIGNLTFYFMQWQDMRLPELYLTAVTILLIIGVLDDRFDISPLLRAGIQAAL
                     AGAMIYSGLSLSSLGQIIAPFSIELGWLGIVFTVFITIGVINAFNMVDGIDGLLAGLS
                     SVSFAGLGTLMFINGEPSLGYWCFALIFVLLPYAMFNLSLFGAKWKVFMGDSGSMLIG
                     FTIIWILLLSTQGQGSPISPITGLWLIAVPLIDMVAVIIRRLKKGKSPFKPDRLHLHH
                     LMMRAGLTSRQALLVITLWAGFCSTIGVLGEVYYWNQWVMMLMFIGLFFLYAYSITHA
                     WRVTRFVRRLKRRAKRSKQL"
     gene            11994..13274
                     /gene="hemL"
                     /locus_tag="PXD66_000979"
     CDS             11994..13274
                     /gene="hemL"
                     /locus_tag="PXD66_000979"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005721785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HDL6319294.1"
                     /translation="MSISEQLFEQAQKVIPGGVNSPVRAFKGVGGTPVFIEKAEGAYI
                     TDSDGKQYIDYVGSWGPMVLGHNHPAIIDAVLKAVPKGLSFGAPTAAEITLAELVCKL
                     VPSIEMVRMVSSGTEATMSAIRLARGYTNRDKIIKFEGCYHGHSDSLLVKAGSGALTL
                     GQPSGPGVPADFAKHTLTCTYNDLDSVKAAFEQYPNEIACLIVEPVAGNMNCIPPQEG
                     FLQGLRDLCTQYGAVFIIDEVMTGFRVALGGAQSYYGVTPDLTTLGKIIGGGMPVGAF
                     GGKKEIMEYIAPTGPVYQAGTLSGNPIAMAAGLACLTELSKAGNETILAEKTKTLAEG
                     FKALADKHNVPLTVQYVGGMFGLFFTEQKAVNNFQEVMKCDAAKFNRFFHLMLEQGVY
                     LAPSAFEAGFMSLAHSDEDIARTLEAADKAFAQL"
BASE COUNT         3901 a         3274 c         2747 g         3617 t
ORIGIN      
        1 acttgctgtc gttctcgatt ttggtaagga ggtgatccaa ccgcaggttc ccctacggtt
       61 accttgttac gacttcaccc cagtcatgaa tcataccgtg gtaaacgccc ccctttcggt
      121 taagctatct acttctggta caacccactc ccatggtgtg acgggcggtg tgtacaaggc
      181 ccgggaacgt attcaccgca acattctgat ttgcgattac tagcgattcc gacttcatgg
      241 agtcgagttg cagactccaa tccggactta gacgtacttt ctgagattcg ctccccatcg
      301 ctggctcgcc gccctctgta tacgccattg tagcacgtgt gtagccctac tcgtaagggc
      361 catgatgact tgacgtcatc cccaccttcc tccggtttat caccggcagt ctcctttgag
      421 ttcccgacct aatcgctggc aacaaaggat aagggttgcg ctcgttgcgg gacttaaccc
      481 aacatttcac aacacgagct gacgacagcc atgcagcacc tgtctcatgg ttcccgaagg
      541 cactctcata tctctacaag attccatgga tgtcaagagt aggtaaggtt cttcgcgttg
      601 catcgaatta aaccacatgc tccaccgctt gtgcgggccc ccgtcaattc atttgagttt
      661 taaccttgcg gccgtactcc ccaggcggtc gatttatcac gttagctacg ggcaccagag
      721 ttaaacccca atccccaaat cgacagcgtt tacggcgtgg actaccaggg tatctaatcc
      781 tgtttgctcc ccacgctttc gcacatgagc gtcagtacat tcccaagggg ctgccttcgc
      841 cttcggtatt cctccacatc tctacgcatt tcaccgctac acgtggaatt ctacccctcc
      901 ctaaagtact ctagttgtcc agtctgaaat gcaattccca ggttaagccc ggggctttca
      961 cacctcactt aaacaaccgc ctgcgtgccc tttacgccca gttattccga ttaacgctcg
     1021 caccccccgt attaccgcgg ctgctggcac ggagttagcc ggtgcttctt ctgtgattaa
     1081 cgtcaatcga ctgttctatt aaaacaatcg ccttcctcgt caccgaaaga actttacaac
     1141 ccgaaggcct tcttcattca cgcggcatgg ctgcatcagg gttcccccca ttgtgcaata
     1201 ttccccactg ctgcctcccg taggagtctg gaccgtgtct cagttccagt gtggctggtc
     1261 atcctctcag accagctaga gatcgcgggc ttggtgagcc tttacctcac caactaccta
     1321 atcccacttg ggctcatctt atggcaggtg gccaaatggt cccacccttt agtcctaaga
     1381 cattacgcgg tattagctac cgtttccagt agttatcccc ctccataagc cagattccca
     1441 agcattactc acccgtccgc cactcgtcac ccaaggagca aactcctatg tgctaccgtt
     1501 cgacttgcat gtgttaagcc tgccgccagc gttcaatctg agccatgatc aaactcttca
     1561 attcaaaaag tttaatcgct caatactgct gacataaaat cgcacttcca aatttaaacc
     1621 taattaaaat taagttaaaa gaaagtaatg aatttctagt tagcacctat taagacttca
     1681 aaattaaaaa aatgtttttc aatcaagtca atcaacaagt gcccacacag attgtctgat
     1741 aaattgttaa agaacaaaaa ataaattggt cggcgagata ggatttgaac ctacgaccca
     1801 ctggtcccaa accagttgcg ctaccaagct gcgctactcg ccgtctgata ctcacaagag
     1861 tattgaatgg ggtggctaat gggattcgaa cccacgacaa ctggaatcac aatccagggc
     1921 tctaccaact gagctatagc caccattgtg ttgatagtgc cgcattgaca tctggcgcgc
     1981 tcgacaagat tcgaacttgc gacctttggc tccggaggcc aacgctctat ccaactgagc
     2041 tacgaacgct tcgatttaca tcaattcaac ttaatgcgtc gcaacggagg cgtatattat
     2101 agatttcact ttcccttgtc tagcactttt taaaaaaaat gtaaaaaaat gtattgtttg
     2161 gttttatttt attcaaaacg tttaataaat atcaaaaaat gcgtttttta gagctgattt
     2221 gtcagatagt aataggcata ttttagtaaa tttaattgtc tttgccagcg ggttggctgt
     2281 ttcaatagtc gataaagcca ttccagcccc aaattttgcc aaattttagg tgctcgtttc
     2341 actttaccgg caaacacatc gtaagtcccg ccaacaccca tatataaggc ttgtggatat
     2401 tgttgttgta actgttggat aaaaagttcc tgttttggag ttcccattgc cacactaata
     2461 aacttcgcgc cacttgcttt aatttgctct ataacgtcct gttcttgctc cgccttaaaa
     2521 tagccattct gcctaccgac aatattcacc ttccattgtg ccaattttgc ttccaccttc
     2581 gttagggtat ccgccgagct gccgaccaaa aacaccggca catttaacgc tccggctctt
     2641 tgcattaagg tttcccataa atcggctccg gcaattcgct ctattttata gtttggatat
     2701 ttttttcgaa tcgattgaac aacactgatg ccgtctgcat agttgaactc ggcttcatct
     2761 atcactcggc gtaacaacgg atttttttcc gacagaatca ctttctcagc attaattgcc
     2821 actaatcttc cgaactgaat ccgcttttca ttcatcagcc aatttgcaaa aatttcccga
     2881 tttttgaccg cttgtaacgc aatgccccga atgatcactt tatcgaacat tattattttt
     2941 ccccattgaa aggattttcg ccagcaaata gcaagccata aatatcagcg tgaaaaagca
     3001 aaaacgggaa acaaatgcat cgattccctc tcgcactaat acgatcaaat tgaataaatt
     3061 gccgaagcag taggcttgca acaaggcgga attgcacgct ttggcgtaag cgaataggtt
     3121 atctaaggct ttaatcatgg cagctactat ccccattccc aacgccataa cgctaaatcc
     3181 gcccataata tagaaagagc cgagtaaggt aggcgaaata gctaaccctg attggttgcc
     3241 taaaatcact ttggtaaaat aattcgccgt attccagacg atctcagggc gttcttgcca
     3301 taaagattgt gggatatata catagaaatc ccgcacaatc ggcattaaac cttgatattc
     3361 cacctcatta gaaaatacac gagccaaatt ttcccaaggc gaaaaagtat ctcggcttaa
     3421 atataagaag gtgtaaatca tctcgctacc ggaaagggta agtttgtaac gggctagagc
     3481 gaggaagaac atcgccacca cgccgattag gcttacggca atcacctttg ataacgacag
     3541 atagccttgc tgcaatccaa tcaataagaa aaagccaacc gctagtgcta aatttgccca
     3601 tgtgccgccc actgccacat agcttaatat gccgaatgcc ccaccaacaa tcagtaacgc
     3661 ccaccacgct gttttattct tatataggaa gcaaaaaatt aacaacgccg gcaataaaaa
     3721 gtagaaaaat cgttttaatg ccgaaagtat gaccctatct ttggcaaaca gttggctata
     3781 tttttcgagt ttgaaaagca gaaaaccgtt taaataaagg aaatatagca gggcaacaac
     3841 tgccacagcg gcaaggatac aagcggtcgt ttttgcctga aaatttgcaa caagagcagt
     3901 tggttgcgac acagcactcc acaagcgtga atagacaacg taataaatca aatagccaac
     3961 acctgctgcc gtaaaaactt gccattgtgt ttttagctcc ggcagcgggt aatcaaagcc
     4021 aaatgccatt attaacgaaa acggaaaacc ggcgtaaaaa gtaacaaaat agagcaaact
     4081 aaacagtaaa tggaaagaaa tccccgattt ctgataaagt tgataaagta agctgaaaaa
     4141 tagcccgaca gaaacaagat aaaatccgcc aagtggtatt aaattattca ttttgcatta
     4201 attcgattaa tcgctgttgc catagtttag tgtagcccgg agcaaaaaac gcaatgcggt
     4261 ttttatctat gctctttagt tgttgtttgg ttgtttgaat ctgtgaagca gtcagatttt
     4321 ccgccaacaa ataaggcact ttttctgcct gtagatccaa cccaaacgga ttggctgaat
     4381 gtaacacaca aggtacatta tggctaatca aaaggcttat cgtgccaatg ccttgctggc
     4441 gtttaaaatt gaaatagccg agatcacaat ggctcaataa cgtaacatac tcttcaaagc
     4501 tcagcttctt ccgcaaaatt tgcaaatttt ccgccaaaaa taaccgcttg ctataggctt
     4561 ccacctgccg aatatagctt tcattatttt ccgggtagcc cattggtatg ataatccgca
     4621 cattgttccc tagtcgttga tgaatctcgc ttaaggcttg aataagctca ttcgctggat
     4681 cgcctgaatt gccgagcaat atcgtcaagc gatcattttg tgtggggcta atttttattt
     4741 gatcacctaa tttcgtgggg aaatagatta aatgatcctg ttctgatctt gtacgcatat
     4801 ttcgccacaa atgatccaga tcgcctcttg ttgcccaaac ctgtaagatt ttttgttggg
     4861 caacacgccg aaacgggtaa aacagcttaa atttccacga agttgccatt tcatataaat
     4921 ccgcccccca aatatgccaa atcaggttgg tggtcggcaa tctgcctaag gctatcgcca
     4981 gccaaatcca agggttaaac tgcccgtgta acagaaaaca agcggtcgga ttttgccgtt
     5041 tttttgcaat taaggcgttc gcaatcgctt tttgtgaaga gaactgctta atccgcaaat
     5101 gaggaaaagc agtttgcagt gaaggcttgc ctaccacata aaaaaaatgt tcggagtctg
     5161 gcaataacgg caaaagctct tgctgaaaaa aggttaaaac ctgttggtta tgatgctcaa
     5221 tctcggagcc tagaatatga taaatcggca acattacacg cttttctctc cccaccatac
     5281 aaacaatatg caagcggtaa gattttagca aaaatttgca acattactgc aacttctttt
     5341 agctttgaaa cactaaaaaa catcacacac ttggttaaaa aatgctatga taaccgagct
     5401 tttttattat tcatttatcc caacgaggac ttattttact atgtcaaacg caatcgctaa
     5461 tgtatctaaa ctgattcaag acaatgatat taaatttgtc ttattaaaat ttactgatat
     5521 taaaggtaaa gaacacgggg tttcgctccc tgtcagctta gttgccaacg atttggaaga
     5581 cttattcgag gaaggcaaaa tgttcgacgg ttcgtccgta gaaggttgga aggcaattaa
     5641 caaagcagat atgttgttaa tgccgattgc tgaaaccgct gtagttgacc ctttcgcccg
     5701 tattcctacc ctttctattc gttgttccat ttacgaccca aacacaatgc agtcttatga
     5761 ccgtgaccct cgctctattg caacccgtgc cgaaaactac ttgaaatcca ccggtatcgc
     5821 cgatagcgta atgtttggac ctgagcctga atttttctta ttcgatgatg tgcgttttag
     5881 caccgaaatg aacaatgtgt cataccgaat tgacgatgta gaagcggctt ggaataccaa
     5941 ccgtaaattt gaagaaggca acaaagccta tcgtccgttg aaaaaaggcg gctactgtgc
     6001 ggttgcaccg attgataccg cccacgatat tcgctccgaa atgtgtttat tgatggaaga
     6061 gatgggttta gtgattgaag ctcaccacca cgaagttgcg acagccggac aaaatgagat
     6121 cgcaacccga ttcaacagcc tgaccttaaa agccgatgaa acccaaatct ataaatacat
     6181 cgtgcaaaat gttgccgtag aacacggaaa aaccgcttgc tttatgccaa aaccgttcgc
     6241 cggcgacaac ggctcgggaa tgcactgtaa tatgtcatta agcaaagacg gcaaaaatgt
     6301 gtttatgggc gataaatacg ccggcttatc tgaaactgcc ctctactata ttggcggtat
     6361 cattaaacac gcaaaagcat taaatgcctt tactaaccca accacgaact cttataaacg
     6421 tttagtgccg ggttttgaag caccggtgtt attggcatat tctgcaagta accgctctgc
     6481 ctctatccgt attccggctg tgaccagtcc gaaagcgacc cgtgtagaag cacgcttccc
     6541 ggatccaatg gcaaacccat acctttgctt tgccgccctg ttaatggctg gcttagacgg
     6601 tgtgatcaac aaaatccacc cgggcgatgc aatggataaa aacttatacg atttaccgcc
     6661 ggaagagtta aaagatattc caacggttgc ctcgtcttta gaatccgctc ttgacgcatt
     6721 aagcaatgat tttgaattct taacacaagg tggcgtgttc acgaaagact ttattgatgc
     6781 ttatatcggt attcgtcgca aagaggtgga acgtgtaaat atgactccgc acccggtaga
     6841 atttgaactt tactattcat agtaacaaca tatagcaaaa aacggctgaa acactcagcc
     6901 gttttttatt gattgaagaa agcaagcact gcttgaataa cacgctcttg ctcctgctta
     6961 ctcaaattat aaaacagtgg caatcttacc aaccgttcgc tttctaaggt agtaaagcga
     7021 tcatcacctg ctaatcgacc taatttctgc cctgccggac tgctgtgcaa cggcacataa
     7081 tgaaacacgg ttaagatatt ctgtgccttc ataaaatcaa taaaagcagt tctctgcttc
     7141 aagtcactta gctttaagta gaacaaatgc ccgttatggc gacaatattc gccaatgcga
     7201 ggaagctcgc acttagctgt ttgggcaagt ggcaaaaagg cttgatagta attgtgccaa
     7261 agggttaaac gctgttggtt gattttatct gcctctataa gctgagcata caaataagcc
     7321 gcttgaattt ccgacattaa aaaacttgaa ccaatatcct gccagcgata tttatctacc
     7381 tcaccacgaa aaaattggct acggttagtg cctttctctc tgatgatttc cgctttttcc
     7441 agcaaatgcc tcattaatca cgatcgcccc accttcaccg ccagcggtat agtttttggt
     7501 ttcgtgaaaa ctatagcaac caatatgccc gatggaacct aacgccctac ctttataaaa
     7561 cgaattgact gcctgtgccg catcttctac cacataaagc ccgtattttt cggctgttgc
     7621 cattattgaa tccatatcac aagcaatgcc ggcataatgc accggcacaa tcactttggt
     7681 ttttggggta atggcttgct caattttgcg ttcatcaata ttcattgtat ccggacgaat
     7741 atccacaaac acaatctttg ccccacgcaa cgcaaacgca tttgcagtag agacaaaagt
     7801 atagctcggc ataatcactt catcgtccgg ctgaatatcc agcagtattg ccgccatctc
     7861 taacgcagcc gtgcaagaag gggtaagcaa cactttgcta cgggccaggt tttcaagcca
     7921 ctgctcacat aagcgggtga atttaccacc gcccgaaagc tgccctgttg ccatcgcctg
     7981 ttctatatag gcttgctccg cccccacaac cgggggttga ttaaacggaa taaaatcagg
     8041 attgttcatc atcgtatttt tccaaagcag ggtaaaacca ataatacacc gccttaatgc
     8101 ttgcaccaaa cgcttgataa aaacgaatcg ctttctggtt actcagctga gtggtaatca
     8161 tcagtttttt cgcattttgc tgtttcgccc acgctatcgc agcatttaac aattccgagc
     8221 caataccttg ttgtcggcaa tcaggcttta ccgccaataa tccgattttc gcttgctgtt
     8281 gggcaagtaa gcgaaccgtt accgcccctt gaatttgccc gtcagcattg cattgaacca
     8341 agccaatatc atcaaacttg ccttttaccg cattttcaat ccaagtccgg taaaagcgtt
     8401 gattctcttc ccttgaaaaa tagggcgaac gaaaccggct atcgggaaat gccgaaccaa
     8461 acaataagtt caagtcgggc aaatctgcca tatccgccgg gcgacaagcg gtcatatttt
     8521 cagaaaatgt tgcaagtgcc agctcaaatt ccaattctcc ctccacaagt acaaaacctt
     8581 gtttttgcaa cctagcgatt tgcctaaaat catcagccgc tacttttact tgcacaattt
     8641 ctctaccgaa aaaatcggac agccattgat ccggcttcat cttacccaca ctcctcgaca
     8701 atcaataata tgccgttgcc tgatctgttc ccttgaaatt tgcttaaatg aagtgtgatc
     8761 cactaacaag actaaaatgt ctgcctgcga aagtgcggtg ttgagatcga ctaaggtgat
     8821 tttttcgcca gccagctgtt gaatatttgg ctcaaccgcc aacaggctac cctgatgcca
     8881 agcggcaagt tgccgggtaa tcgctaatgc cgggctttcc cgcaaatcat caatattcgg
     8941 cttgaaagct agccctaaac aagcgatggt tagctcactt ggcttacaat cttgctccat
     9001 cgcacagtct gcgaccgatg ttttcacttg ctcgattacc cattgcggtt tgctgtcatt
     9061 cacttctcgt gccgtgcgaa taaggcgagc ctgttggggc gatgtggaaa cgataaacca
     9121 cgggtcaacc gccaaacaat gtccgcccac ccctgctccg ggctggagaa tatttactct
     9181 cgggtgaaga tttgccaatt caattagctc ccacacattg atcttcaacg catcacaaat
     9241 cattgaaatt tcattggcaa aggcaatgtt cacatcacga aagctgtttt ccaccaactt
     9301 gcacatctcc gctgtgcgag ctgtggtggt gacacaacgt ccttttgcaa aaatttggta
     9361 taaatcgacc gcttgttgac cgcattgttc gcttaatccg ccgataatgc ggtcattttc
     9421 aaccaattcc gtcattatat tacccggcaa aacacgttca gggcagtagg caatagacac
     9481 atcggctgga aaggctaaat cagggcgagc ttggcttagc cattcggcta aattttctgt
     9541 tgtaccgaca ggcgaagtgg actccagcac caccaaattc cctttacgca aacaaggggc
     9601 aatcatagcg gcggcatttt gaatataagt gagatcgggc tgcttcccgc aaagtggggt
     9661 tggcacggcg atgataaaag catcggccgg ttgcggtttg gcagttgcaa aaaaattacc
     9721 ttttttgacc gcttgtgtga aggcagtttg taaatccggc tcgataatat gaatgttgcc
     9781 ttgattaagg cttgccacca cttgcggatt aatatccacc ccaattactt gcttgccggc
     9841 attggcaaaa acgactgagg tcggcaagcc gatatagcct aaccccagaa tacaaatagt
     9901 gttaaattca gttttactca tcatcagtta caaagagaaa tggcacacta aattgtgtgc
     9961 cattttagat taaagttccg caggttgagc tttcgctttc accgcaaaac tgagcataaa
    10021 gcccagcagt aagccactta atgccccaat catcagccaa aataggcgga tcaggcgatt
    10081 ctgatttaag tggaacgcta ggcgatttaa ccaagcgata agctgtaagt tggttgtcta
    10141 acggctgaac agaacgcatc atttgcagtt tagctgccca atcttgtgag ccttgcttac
    10201 cgttaatatt caactccgaa gcagttttaa tttgctggaa caagactttc catttcgcaa
    10261 ttaaatcggc gtttaattta tctcgagctt gggtgtttac caacggaata aaatcggcta
    10321 ataatttttg agcctcttcc gcattttctg caattaagct gacattatct gccgaagttg
    10381 ccatatcttt ttcaaaacgg aattgttcaa ctaattcagc cgccaccacc gccggtgatt
    10441 tctttgcaac ctgagctttt aatttcaccg cttctgtttg tagcaaatag gcttgaagca
    10501 agtcggcgga cgtgaggttg cgtttaaatt ccgcataaac ctgctcactt gcctgttgct
    10561 gtgccgtttg gctattcgct tcttggctat tactattcaa aaaatggtag gtggaaagta
    10621 acgagtaata attacctaaa tcgggaatag tcggttgctc aaattgagcg gtcactttcc
    10681 atttttcagg ttgagtataa ctcagcccat aaccggcacc tgccccgata gcagcaaata
    10741 aaattagcac taaaagtcga taaaataagg ttttcacaac atatccttta aagttgtttg
    10801 ctacgttttg cacggcgttt tagacggcga acaaaacggg ttacacgcca agcgtgggta
    10861 atggaatagg cgtataggaa aaacagcccg ataaacatta gcatcatcac ccactgattc
    10921 caataataca cctcgcctaa taccccaatg gtggaacaaa atcccgccca gagggtaatc
    10981 accaataatg cttggcgaga ggtaagccct gctcgcatca ttaaatggtg taggtgcaag
    11041 cggtctggtt tgaacggact tttacctttt ttcaaacggc ggataatcac cgcaaccata
    11101 tcaattaacg gcacggcaat cagccacaaa ccggtaatcg ggctaattgg cgagccttgc
    11161 ccttgggtgc ttaacagcag aatccaaata atggtaaagc cgatcagcat actgcctgag
    11221 tcgcccataa acactttcca tttcgcccca aacaggctca aattgaacat tgcatacggc
    11281 aacaacacaa agataagggc aaagcaccaa tagcccaaac ttggttcgcc attaatgaac
    11341 attaaagtgc caagaccggc aaaactcacg ctggaaagcc ctgctaataa gccatcaatc
    11401 ccatctacca tattaaacgc attaataacg ccaatggtga taaacacggt aaagacaatg
    11461 ccgagccaac ctaattctat actaaagggg gcaataattt gccctaaact gctgagcgac
    11521 aagccactgt aaatcatcgc cccagctagt gctgcttgaa tcccggcacg cagtaatggg
    11581 ctgatgtcaa atcggtcatc taacacgccg ataatcagca aaatagttac cgctgtcaga
    11641 tacagttccg gcagccgcat atcttgccac tgcataaaat agaaagtgag attgccgata
    11701 aataatgcaa taccaccgat taacggaatc aacccctgat ggcgtttacg gaagttgggc
    11761 ttatctacca aaccaacaaa ttccgccacc ggacgcatca caatcagggc taaaaaagaa
    11821 acaataaata cggttaaaaa agtaagccac atacagggat tgcctcaaaa aatgagtcat
    11881 aaaataggaa tgcagaatag cataaggcaa caaaatcgta aattttttag cgaaaacaag
    11941 ggcttttcag tacaataaca ggcaaatttt tacatcaaaa gaaaggaatt gatatgtcta
    12001 tttcagaaca actgtttgaa caagcccaaa aagtgattcc gggcggtgtg aactcccctg
    12061 tgcgtgcatt taaaggcgtg ggcggcacac cggtttttat tgaaaaagcc gaaggtgctt
    12121 atattaccga cagcgacggc aaacaatata tcgactatgt cggctcttgg gggccaatgg
    12181 tactcgggca taaccacccg gcgattattg atgcggtgct aaaagccgtg ccaaaaggct
    12241 taagtttcgg tgccccaacg gcagcagaaa tcacacttgc cgaattagtg tgcaaattag
    12301 taccttctat tgaaatggtg agaatggtga gcagcggaac agaggcaaca atgtctgcca
    12361 ttcgtttggc tcgtggctat accaaccgag ataaaatcat caaatttgaa ggctgctacc
    12421 acgggcattc cgattctctg ttagtaaaag ccggttcagg tgctttaacc ctcggtcagc
    12481 caagcggccc gggcgtaccc gctgatttcg ccaagcatac gcttacttgc acctataatg
    12541 atttagattc agtaaaagca gcatttgagc aatacccaaa tgagatcgct tgtttaattg
    12601 ttgagcctgt tgccggcaat atgaactgca ttccgccaca agaaggcttt ttacaaggct
    12661 tacgtgatct ttgcacacaa tatggtgcgg tatttattat tgatgaagta atgaccggct
    12721 tccgtgtcgc actcggtggg gctcaatctt actatggggt aacaccggat ttaaccacac
    12781 tcggcaaaat tatcggcggc ggaatgccgg tgggggcatt tggtggtaaa aaagagatta
    12841 tggaatacat cgctccaact ggcccggttt accaagccgg cacgctctca ggcaacccga
    12901 ttgcaatggc tgcaggtttg gcatgcttaa ctgaattatc aaaagcagga aatgaaacca
    12961 ttttagcgga aaaaaccaaa accctcgctg aaggctttaa agctcttgca gataagcaca
    13021 atgtgccatt aacggtacaa tatgtcggcg gaatgttcgg cttattcttc accgaacaaa
    13081 aagcagtaaa caacttccaa gaagtaatga aatgtgatgc cgccaaattc aaccgtttct
    13141 tccacttaat gctggaacaa ggcgtgtatt tagccccatc ggcattcgag gcagggttta
    13201 tgtctttagc tcacagtgat gaagatattg ctcgtacgct agaggctgcg gataaggcat
    13261 ttgctcagct ttagaagaat gacaagcggt tgggtttact caataatttg caagtttttg
    13321 agggaatttg accgcttgta gtgcaaattt ctcttacgat agcaattatg atggtgttcg
    13381 tctctgacga gtgcttatac aaactttcat tatgcttaaa caagcacccg tctggagacg
    13441 ggcgctatcg agggaatagg attgataaag caatatatgg gcttccaaca cttgcaggca
    13501 aggacaagtc ttacactttc taatattaca aatatattt
//
LOCUS       DANLXF010000055        13312 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.055, whole genome shotgun sequence.
ACCESSION   DANLXF010000055 DANLXF010000000
VERSION     DANLXF010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13312
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.055"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            310..954
                     /gene="rppH"
                     /locus_tag="PXD66_000980"
     CDS             310..954
                     /gene="rppH"
                     /locus_tag="PXD66_000980"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HDL6319295.1"
                     /translation="MIDFDGYRPNVGIVICNKHGQVLWAKRFGQNSWQFPQGGINEGE
                     NIEAAMYRELYEEVGLSKKDVRLLWASKYWLKYKLPKRLVRNEGSGPVCIGQKQRWFL
                     LQLIGDESLINLKTTKNPEFDGWRWVSFWYPVRQVVSFKRDVYRKAMKEFAQVLQNNI
                     QKATDDKTGEKPRIERGETSPHTYKKTSQDSAKAYKREARKPYRPWHQHNQKGK"
     gene            956..1744
                     /locus_tag="PXD66_000981"
     CDS             956..1744
                     /locus_tag="PXD66_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDL6319296.1"
                     /translation="MTILLSCLFLGVFVGFLAGLFGIGGGLIIVPSLVFLLPMAGVPP
                     EYIMSTALGTSFSTIIITAFASAQRHHKLGNVDIQTSKYFIPALMVSVFLSGLVVSRL
                     DPQLMSRIFAVMVLYLAGKMIFSIKRAPKNKPLTVQTTIIGGSIIGALASMAGIAGGA
                     FIVPFLNDRGLEMKRAIGTSSFCGAFLGLSGTISFIVSGWGLEGMPDYSLGYVYLPAL
                     FGITATSFFTSKMGASAANALPVPMLKKAFAVLLVCIAINMFLK"
     gene            1752..2549
                     /gene="lgt"
                     /locus_tag="PXD66_000982"
     CDS             1752..2549
                     /gene="lgt"
                     /locus_tag="PXD66_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDL6319297.1"
                     /translation="MNEQFIQFPQISPIVFEIGPIALRWYGLMYLAGFAFAYWLGMRR
                     AKNSNGVWTTEQVDSLVYTCFWGVVLGGRIGDVFFYSFDRFLQDPLYLFRIWEGGMSF
                     HGGLIGVIVAMIWVSYRQKRNFWQTADFIAPLVPFGLGMGRIGNFINDELWGRVTDVP
                     WAVLFPSGGYLPRHPSQLYEAFLEGVVLLFILNWFIQKPRPAGAVSGLFLIGYGTFRF
                     IVEYFREIDHNVNTTADLITRGQLLSLPMIIGGILIMAWAYKVNKVK"
     gene            2636..3304
                     /locus_tag="PXD66_000983"
     CDS             2636..3304
                     /locus_tag="PXD66_000983"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HDL6319298.1"
                     /translation="MSKKYKVIGFDLDGTLINTLPDLTLVANSMFLEAGLPTVTQEKV
                     LTWIGKGADIFFQNAVAHTGRIFQADELVKMRVSFDKYYATYVCEESTLFPNVKETLE
                     ILKANDYPLVVITNKPTKLVEPVLSAFGIYHLFDETLGGQSLPKIKPHPDPMFFICEK
                     FGITPSELLFVGDSENDVIAAKAAGCDVVGLTYGYNYNVPIEQSNPTFVTSEFKDVLK
                     IVGL"
     gene            3374..3634
                     /locus_tag="PXD66_000984"
     CDS             3374..3634
                     /locus_tag="PXD66_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HDL6319299.1"
                     /translation="MPTIALRQVGGSVAMVIPPAFLEALKLSVGSKVDLSLNDNQLVI
                     NPARPKVKYSLEELLRQSDFEAARNEESQSWLSEPAIGKEIL"
     gene            3631..3963
                     /locus_tag="PXD66_000985"
     CDS             3631..3963
                     /locus_tag="PXD66_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL6319300.1"
                     /translation="MKRGDIYWLDLDPTLGHEQSGFRPVVIVSVTAFNLASKLPVVVP
                     ITNGGAFAERLGFAVSLKDAGLKTTGIIRCDQPRTVDIAQRHGKFIEALPESLLDEIM
                     ARVSVIFE"
     gene            4090..5121
                     /gene="trpS"
                     /locus_tag="PXD66_000986"
     CDS             4090..5121
                     /gene="trpS"
                     /locus_tag="PXD66_000986"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDL6319301.1"
                     /translation="MSKPIVFSGVQPSGELTIGNYLGALRNWVKMQDDYDCVYCIVDQ
                     HAITVRQDPESLRKSTLDVLALYLACGIDPNKSTIFIQSHVPEHAQLAWVLNCYTYFG
                     EMGRMTQFKDKSARYEENVNVGLFTYPVLMAADILLYQANQVPVGDDQKQHLEITRDI
                     ANRFNALYGKKDSEGNLIEPVFAVPEVFIAKSGARVMSLLEPTKKMSKSDDNRNNVIG
                     LLEDPKAVAKKIKRAMTDGDEPPVVRYDVQNKAGVSNLLDILSAITGKTIAELETEFE
                     GKMYGHLKTEVADKVSELLTNLQERYHHYRQDEALLEKIYREGAEKARAQAKATLDKV
                     YELVGFVRY"
     gene            complement(5185..5940)
                     /locus_tag="PXD66_000987"
     CDS             complement(5185..5940)
                     /locus_tag="PXD66_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HDL6319302.1"
                     /translation="MKFIKKLSYSVFLATVITACATTESINQEAAQSYAQVKTQARQQ
                     NAIDNSSATAKRIHTVFHKMKPYAERANQTGVPFHWEITVLKSDELNAWAMPGGKMAF
                     YTGLVERLNLSNDEIAVVMGHEMAHALKEHGKSDRTVSAITSIVGAIADVAVTASTGV
                     STEGLLSTGVDLIATKPFSRSQETEADEVGLILMAESGYNPSAAPNVWVKMSKANGDS
                     GLSIFSTHPSNADRQENLARLVPEAMKIYNARK"
     gene            complement(5996..6523)
                     /gene="ppa"
                     /locus_tag="PXD66_000988"
     CDS             complement(5996..6523)
                     /gene="ppa"
                     /locus_tag="PXD66_000988"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HDL6319303.1"
                     /translation="MGLELVPAGKELPDDIYVVIEIPANSDPIKYEVDKETGALFVDR
                     FMATAMFYPANYGYVNNTLSSDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGG
                     DAKVVAVPHTKLSKEYDHIKDVGDLPALLKAQIQHFFESYKALEAGKWVKVEGWGDVN
                     EARQEILESFERAKK"
     gene            6740..6815
                     /locus_tag="PXD66_000989"
     tRNA            6740..6815
                     /locus_tag="PXD66_000989"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6773..6775,aa:Lys,seq:ttt)
     gene            6838..6913
                     /locus_tag="PXD66_000990"
     tRNA            6838..6913
                     /locus_tag="PXD66_000990"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6871..6873,aa:Lys,seq:ttt)
     gene            6935..7010
                     /locus_tag="PXD66_000991"
     tRNA            6935..7010
                     /locus_tag="PXD66_000991"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6968..6970,aa:Lys,seq:ttt)
     gene            7103..8137
                     /gene="mltG"
                     /locus_tag="PXD66_000992"
     CDS             7103..8137
                     /gene="mltG"
                     /locus_tag="PXD66_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDL6319304.1"
                     /translation="MLKKILIALGLAGVIACGGLFYGYQKLTSLAEHPITVQPNQLFV
                     LEKGVSSQKLAALLEEQGIVTHDDADLIPYLMRLYPELSKFKAGAYSLAGLTTVKDLL
                     AHLSSGKEVQLNVQFIEGKTFKIWREQLAKADYLQHTLQGKSEKEIADLLGIPHEKIE
                     GWIAPDTYNYTPNSTDLALLKRAYQKQKTALEQAWKNRAENLPLATPYEMLILASIVE
                     KETGIATERPQVASVFINRLRNNMRLQTDPTVIYGMGDRYNGNIRRKDLEEATPYNTY
                     QIDGLPPTPIAMPSEASIKAVANPDNTPYLYFVADGTGGHKFSKTLNEHNKAVAEWIQ
                     IERKRKNEQK"
     gene            8148..8768
                     /locus_tag="PXD66_000993"
     CDS             8148..8768
                     /locus_tag="PXD66_000993"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDL6319305.1"
                     /translation="MRGKFIVIEGLEGAGKTTAHQVILKELEQAGINDVVMTREPGGT
                     PLAEKLRHLIKHETEEAINDKAELLMLYAARVQLVENVIKPALAEGKWVLGDRHDMSS
                     QAYQGGGRKLDRHLLETLKESVLGTFEPDLTIYLDLDPVIGLERARGRGELDRIEQQS
                     LDFFYRTRERYLELTQHNDKAVIINAEQPIEQVTADIQQVIRNWLK"
     gene            8768..9745
                     /locus_tag="PXD66_000994"
     CDS             8768..9745
                     /locus_tag="PXD66_000994"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDL6319306.1"
                     /translation="MYPWLESTYQQLTDSFLQGRGHHALLFKTDVGLGTDTLIYKVAE
                     WLLCSQKLRHQPCLECKSCLLMQSNNHPDFHILEPIEDKDIGIDQVRELIYKLQNFAQ
                     QGGNTVVYIRGVDRLTEASSNALLKTLEEPRENVYFLLEAPLQATVLATIQSRSQTWV
                     LYAPQASEVMAWLQTQFPAISTDDLEIALRLCHSRPLICKNFIENDRLQQRKTFLQTF
                     WRFYKSHDVFLLFQAFAQEKELILQQLEWLESFFSDALKAKMAISHGWLNPDLQNGIL
                     PFSQQLTTHGLLKGHQILQQTQRDLTEINAVNPELMLLDCLTKLVLVFE"
     gene            9876..10118
                     /locus_tag="PXD66_000995"
     CDS             9876..10118
                     /locus_tag="PXD66_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HDL6319307.1"
                     /translation="MARTTSVTLGEPLNDFVNEMVASGRYGSTSEVVRTALRMLEEKE
                     QYLAQLRQAIDDGDASGYATETIDEMMLRVKAELNV"
     gene            10111..10395
                     /locus_tag="PXD66_000996"
     CDS             10111..10395
                     /locus_tag="PXD66_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL6319308.1"
                     /translation="MYKLSAQALRDQEKLFRYSISQFGVQQTESYFADLKHILNLLAD
                     FPTMGTSAEYIRGGLRMHTHQSHTIYYRIKADHIFIVRILHNQMNHKRYI"
     gene            10400..11188
                     /locus_tag="PXD66_000997"
     CDS             10400..11188
                     /locus_tag="PXD66_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchF/TatD family DNA exonuclease"
                     /protein_id="HDL6319309.1"
                     /translation="MKDLFIIDSHCHLDSLDYETRHKNVDEVIENAKARGVHHFISVC
                     TTVGRFEAMKNLTAHRNDVSLSCGVHPLNVEDEPFDYDKLFQFAQDPKVVAIGETGLD
                     YHYTPETKALQQSLFVQQIEIANKLNKPLIIHSRSARQDTMDMLEQNNAEKCGGVLHC
                     FTEDWAMAKRALDIGFYISISGIITFRNAEELREVVRKVPLDRLLVETDSPYLAPIPY
                     RGKPNQPAYVRETCEYVATLKGVSTEELARITTENVQHLFNIKL"
     gene            11198..11719
                     /locus_tag="PXD66_000998"
     CDS             11198..11719
                     /locus_tag="PXD66_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1523 family protein"
                     /protein_id="HDL6319310.1"
                     /translation="MRAILKYFLILVSLSFFIVLGGAVNYAMPSYEDTVVTGMEVRRM
                     DKDGIISKANPADGEVRDVYFLFTEDPETKKVMVYRNEDTGWGLPPYFKFGSADIQAK
                     AQAYANEKQRVQIKYYGWRINWLNEFRNIVSIKPLEEAETVSKPIMSYVLYAVLAFLF
                     FLSVQFIRGIFKD"
     gene            11794..12495
                     /gene="lpxH"
                     /locus_tag="PXD66_000999"
     CDS             11794..12495
                     /gene="lpxH"
                     /locus_tag="PXD66_000999"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HDL6319311.1"
                     /translation="MTYYFIADLHLNENQPEITHLFLQFMQEKALLAEAVYILGDLFD
                     FWIGDDEQSELISTVKQAIKTLTTSGTKCYFICGNRDFLIGKRFSEQTGMVILPDYHV
                     IDLFGTSTLLCHGDTLCTDDVKYQQFRKKVHQKWRQAIFLMFPLSWRIRIAEKIRSKS
                     KQEKRDKSAEIMDVNAEVTAQIVAKYNTTQLIHGHTHRQAIHQHNGFTRIVLGDWKEN
                     YASILSVTEQGAEFI"
     gene            12492..13282
                     /locus_tag="PXD66_001000"
                     /pseudo
     CDS             12492..13282
                     /locus_tag="PXD66_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618283.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
BASE COUNT         3952 a         2833 c         2772 g         3755 t
ORIGIN      
        1 tgctttattt gcgaagcaaa aattgccctg cttattcatt ttaaaattta aacttatttt
       61 gacgagaaaa aacgcaacaa cttgatcttt atccagatca ttgcttaaga aaaagatcgc
      121 atttttagat ctgattaatt gctctgtttt acaacgttcc gcctgtttat cctcaactta
      181 taccacttat attcaatagt tatccacaaa gttattcaca agcggttata ttttatggtt
      241 tttttgcaaa tcaacccaaa gtatgaaaca attctcccat tctagaattt ttatcaatta
      301 ggtggtcgag tgatcgattt cgatggctac cgcccaaatg ttggaatcgt tatctgtaat
      361 aagcacgggc aagttctgtg ggcaaaacgg tttggtcaaa actcttggca atttccgcaa
      421 ggtggaatta atgaggggga aaacattgaa gcagcaatgt accgtgagct ttatgaagag
      481 gtcggcttaa gtaaaaaaga tgtacgattg ctttgggcgt ctaaatattg gctaaaatat
      541 aaactgccaa aacgcttggt acgtaatgaa ggctctggtc ctgtctgtat cgggcaaaaa
      601 cagcgttggt ttctactgca attaatcggt gatgaaagcc tgattaattt gaaaaccacg
      661 aaaaatccgg agtttgacgg ctggcgttgg gtcagctttt ggtatccagt ccgccaagtg
      721 gtctctttta aacgagatgt ttatcgcaaa gcgatgaaag agtttgcaca ggttttgcaa
      781 aacaatattc aaaaagcgac tgacgataaa accggtgaga aaccacgcat tgagcgtggc
      841 gagacttcgc cccatactta taaaaaaacc tctcaggatt ctgccaaagc ctataaacga
      901 gaagctcgta agccttatcg tccttggcat caacataatc aaaaaggaaa ataagatgac
      961 tattttatta agctgccttt ttttaggggt gtttgtcggc tttttagccg gcttgttcgg
     1021 cattggtggt gggttaatca tcgtgccgag tttggtcttc ctactgccca tggcaggtgt
     1081 accgccggaa tatataatgt ccaccgcact cggcacatca ttttcaacca ttattattac
     1141 cgcctttgca tcggcacaac gccatcataa gttaggcaat gtcgatatcc aaaccagtaa
     1201 atattttatt ccagcgttga tggtttcggt ttttctatcg ggcttggtgg taagccgcct
     1261 cgatccgcaa ctgatgtcta gaatttttgc ggtaatggta ctttaccttg caggtaaaat
     1321 gattttttca ataaaaagag caccgaaaaa taaaccactt accgttcaaa cgacgataat
     1381 cggaggttca atcatcggtg cgttagccag tatggcaggg atcgcaggtg gagcctttat
     1441 tgtaccattt ttaaacgatc gaggcttaga aatgaaacgt gccatcggca cttcctcatt
     1501 ttgcggagca tttttaggtt tatctggcac aatcagcttt attgtaagtg gctggggctt
     1561 agaaggaatg cctgattatt ctctcggtta tgtttattta ccggctctgt ttggtattac
     1621 ggcaacttca tttttcacct caaaaatggg ggcaagtgcc gccaacgctc tacctgtgcc
     1681 gatgctgaaa aaagcctttg cggtgctttt ggtctgtatc gccatcaata tgtttttaaa
     1741 ataaggacac tatgaacgaa caatttatcc aatttccaca aattagcccg attgtgtttg
     1801 aaatcggtcc gattgccctg cgttggtatg ggctaatgta tttagccggc tttgcctttg
     1861 cctactggct gggaatgaga cgggcgaaaa attccaatgg agtttggaca accgagcaag
     1921 tggatagctt agtctatact tgcttctggg gcgtagtatt aggtgggcgt atcggcgatg
     1981 tgttctttta tagcttcgac cgtttcttac aagatccgct ctacctcttt cgcatttggg
     2041 aaggcggaat gtcctttcac ggcggtttaa tcggcgtcat tgtagcaatg atttgggttt
     2101 cctaccgcca aaaacgcaat ttctggcaga cagcagattt tatcgcaccg ctagtaccgt
     2161 tcgggctagg tatggggcgt atcggcaatt tcattaacga tgaactttgg gggcgtgtaa
     2221 ccgatgtgcc ttgggcggtg ttatttccaa gcggtggcta tcttccccgc cacccgtcac
     2281 agctttatga agccttttta gagggtgtgg tgttactctt tatcctcaac tggtttattc
     2341 aaaagcctcg cccggcaggt gcggtttcag ggttattttt aatcggctac ggcacattcc
     2401 gctttattgt ggaatacttc cgagaaatcg accataacgt caataccacc gcagatttaa
     2461 tcactcgtgg gcaactactc tctttaccga tgattatcgg cggcatactg attatggcgt
     2521 gggcgtataa agtaaacaaa gtgaaataaa cgtaggggca agtcaatatt tgccctcatt
     2581 acaaaaattg caaaaaattt gcagaaaacg accgcttgtg tttgaaggat atactatgag
     2641 taaaaaatac aaagtcatcg gtttcgactt agacggaacg ctaatcaaca ccctccccga
     2701 tctcactctt gtcgccaatt caatgtttct agaagccggt ttgccaacgg ttacacaaga
     2761 aaaagtgctg acttggatcg gtaaaggggc agatattttc ttccaaaatg cggttgctca
     2821 caccggcaga attttccaag ccgatgagtt ggtgaaaatg cgagtgagct ttgataaata
     2881 ctacgccacc tatgtgtgcg aggaaagcac ccttttccca aatgtgaaag aaaccttaga
     2941 aatcctcaaa gcaaacgact acccgctggt ggtgattacc aacaaaccga ccaaattagt
     3001 cgagccggtg ctttccgctt tcggcattta tcatttattc gatgaaaccc ttggtgggca
     3061 atcgctcccc aaaatcaaac cgcaccctga cccgatgttt tttatctgcg aaaaattcgg
     3121 cattacccca tcagaattac tttttgtcgg cgactcagaa aatgacgtga ttgccgccaa
     3181 agccgccggt tgtgatgtgg ttggactaac ttacggctat aactacaatg tgccgattga
     3241 acaatctaac ccgacttttg tgacatctga atttaaagat gtgttgaaaa ttgtggggtt
     3301 ataaagaaaa catttgtttt cttttgatgt ttgctttaga ctttccgaac ctaaagattt
     3361 aaaggagtaa agtatgccaa caatcgcatt acgtcaggtc ggtggctcgg ttgcaatggt
     3421 gattccgcct gcttttttag aggcactcaa gctctccgtc ggtagcaaag tggatttgtc
     3481 tttaaacgac aaccaactcg tgattaatcc tgcccgccca aaagtgaaat acagcttaga
     3541 ggaattatta agacaatccg attttgaggc agcaaggaat gaagaaagcc aatcgtggtt
     3601 atccgagccg gcaataggaa aagaaatttt atgaaacgtg gcgatattta ctggctcgat
     3661 cttgatccaa ccttaggaca cgaacaatca ggcttccgcc ccgttgtaat tgtttccgta
     3721 actgcattta atctagccag taaactgcct gtggttgtgc cgattactaa cggtggagct
     3781 tttgctgaaa gactgggctt tgccgtttca ttaaaagatg ccggcttaaa aacaacaggg
     3841 attattcgat gcgatcaacc tcgcacagtc gatattgcac aacgccatgg aaaatttatc
     3901 gaagccttac ccgaaagcct acttgatgaa attatggcaa gggtttcagt gatttttgag
     3961 taagatacaa gcggtctgat ttgctctatt ttttgcaaaa tcatcaataa atctgaccgc
     4021 ttgtactttt atcccttccc aataaccgtt aagataacct atatttttca aatcaaacga
     4081 gaagataaaa tgagcaaacc tattgtattt agtggcgtgc agccctcagg tgaattgact
     4141 atcggtaact acttaggggc gttacgcaac tgggtgaaaa tgcaagatga ttatgactgt
     4201 gtatattgca ttgtcgatca acacgccatt accgttcgtc aagatccgga gtcgctacgc
     4261 aaatcaactt tagatgtgtt agccctgtat ttagcttgtg gcattgatcc aaacaaaagc
     4321 actattttta ttcaatccca cgtgccggaa cacgcccaat tagcttgggt gctgaactgt
     4381 tacacctatt tcggtgaaat ggggcgtatg acgcaattta aagataaatc agcacgctat
     4441 gaagagaatg tcaatgtggg cttatttact tatccggtat taatggcggc agatattctg
     4501 ctttaccaag ccaaccaagt gccggtaggc gatgaccaaa aacaacattt ggaaatcacc
     4561 cgtgacattg ccaaccgttt caatgcctta tacggcaaaa aagattccga aggcaacctg
     4621 atcgagcctg tttttgcagt gccggaagtc tttattgcga aatccggggc aagagtgatg
     4681 tcgctacttg agccgaccaa aaaaatgtca aaatcggacg ataatcgtaa caatgtgatt
     4741 ggactattgg aagatccgaa agcggtagcg aagaaaatca aacgggcaat gaccgatggc
     4801 gatgaaccgc cagtagtgcg ttatgacgta cagaataaag ccggtgtctc gaatttattg
     4861 gatattctct ccgccattac cggcaaaacc attgccgaat tagaaaccga atttgaaggc
     4921 aaaatgtatg gtcatttgaa aacagaagta gcagataagg tttccgaact attaaccaat
     4981 ctgcaagaac gttatcacca ctaccgccaa gatgaagcat tattggaaaa aatctaccgt
     5041 gaaggggcag aaaaagctcg agctcaagcc aaagcaacgt tagataaggt atatgaatta
     5101 gtcggttttg tgcgttacta acacttaatg aaaaatgctc ttgaggattc aagagcattt
     5161 ttgcaaaaaa gaaccgattg tcaattattt acgggcgtta taaatcttca ttgcttccgg
     5221 cacaaggcgg gctaagtttt cttgacggtc agcatttgat gggtgagttg agaaaatgga
     5281 caagcctgaa tcgccattgg ctttactcat ttttacccaa acattcggcg ctgctgatgg
     5341 gttgtagcct gattctgcca ttaaaattaa cccaacttcg tccgcctcag tctcttggct
     5401 gcgagagaat ggcttggtgg caatcaaatc cacgccagtg ctgagtaaac cttcggtgct
     5461 tacgcctgtt gaggcagtca cagccacatc tgcaatagca cctacaatac tggtaatagc
     5521 gctaaccgta cggtctgatt taccgtgttc tttcagtgcg tgagccatct cgtgtcccat
     5581 taccactgca atttcatcgt tcgataaatt aagtctttcc actaaccccg tgtaaaacgc
     5641 cattttaccg cccggcatcg cccacgcatt gagttcatcc gatttcaata cggtaatttc
     5701 ccaatgaaaa ggtacaccag tctggttggc tctttccgca taaggcttca ttttatgaaa
     5761 aacagtgtga atccgcttag cggtggcaga cgaattgtca atcgcatttt gctgacgagc
     5821 ttgggttttc acttgagcat aactttgagc cgcttcttgg ttaatgcttt ctgttgtggc
     5881 acaagcggta atcactgttg ctaaaaaaac tgaataactt aattttttga tgaatttcat
     5941 tttatatccc acaaaaaatg ccaaacaaat ttgcttggca ttcaaaaata aataattatt
     6001 tcttagcacg ctcgaatgat tctaagattt cttggcgagc ttcgtttaca tcgccccaac
     6061 cttctacttt cacccatttg cccgcttcta atgctttata gctttcgaag aagtgctgga
     6121 tttgtgcttt taataacgcc ggtaagtcac ctacatcttt gatgtggtca tactctttac
     6181 ttaatttggt gtgtggaaca gcaaccactt ttgcatcacc gccggcttca tcggtcattt
     6241 ttaacacacc gacaggacgg caacgaatca ccgaacccgg ttgtaacgga tatggtgttg
     6301 gcactaatac atcaaccggg tcaccgtctg atgataaggt gttattcaca taaccatagt
     6361 ttgccggata gaacattgcg gttgccataa agcgatccac aaataatgcc ccggtttctt
     6421 tgtccacttc atatttgatt ggatcagagt ttgccggaat ttcaattaca acataaatat
     6481 cgtccggtaa ttctttaccg gcaggaacta attctaagcc cattttggtt tccttctata
     6541 ataggtgaaa taaacggcgt gattatagcc tattttcaga aaaagataaa agcccgtttc
     6601 tcttttcatt ttccgccaac aaatccggct aattttttgc taaattgctt acaaaccgca
     6661 cactcaacca tttttcgata aaaaaaactt gccaaagttt taaaaatccg taagatatgc
     6721 cctcgttacg caagattatg ggtcattagc tcagccggta gagcagcgga cttttaatcc
     6781 gttggtcgaa ggttcgaatc cttcatgacc caccattaat cttcaaaaca cctctaaggg
     6841 tcattagctc agtcggtaga gcagcggact tttaatccgt tggtcgaagg ttcgaatcct
     6901 tcatgaccca ccattaattt caaaactcct ctgagggtca ttagctcagt cggtagagca
     6961 gcggactttt aatccgttgg tcgaaggttc gaatccttca tgacccacca tttactcaaa
     7021 tcccatttgc aaatttttct gaaaaaacaa ccgctttata gtagaattca gtcaattttt
     7081 atcactttta ttttattcat caatgctaaa aaaaatcctt attgcattag gcttagccgg
     7141 cgttattgct tgcggtggac tgttttacgg ctaccaaaaa ctcacctctt tagccgaaca
     7201 cccgatcacg gttcagccga atcaactttt cgtattggaa aaaggcgttt ccagccaaaa
     7261 attagcggct ctgttagaag agcaaggcat tgtcacccac gatgacgccg atcttatccc
     7321 ttatttaatg cgattatacc ctgagttaag caaattcaag gctggtgctt attcactcgc
     7381 aggcttaacg acagtaaaag atttgctcgc tcatttaagc tccggcaaag aagtgcaact
     7441 taacgtgcaa tttattgagg gcaaaacttt taaaatttgg cgggaacaac tagcgaaagc
     7501 cgattattta cagcacacgc tacaaggcaa atcggaaaaa gaaatcgctg acttactcgg
     7561 cattccacac gaaaaaatcg aaggttggat agccccggat acttacaact acacgcccaa
     7621 ttccaccgat ttagccttgc ttaaacgggc ttatcaaaag cagaaaacgg cgttggaaca
     7681 ggcttggaaa aatagagcgg aaaatctacc gcttgcaaca ccatacgaaa tgctgattct
     7741 tgcctcgatt gtggaaaaag agaccggcat tgcgaccgaa cgcccacaag tggcatcggt
     7801 ctttattaac cgtttacgca acaatatgcg actgcaaacc gatccaaccg tcatttacgg
     7861 aatgggcgat cgttataacg gcaatatccg ccgcaaagat ttagaagagg caacgccata
     7921 caatacttac cagattgacg gcttaccgcc aacgccgatt gccatgccga gtgaagcttc
     7981 gattaaagcg gttgccaacc cggataatac accctacctc tattttgtgg cagacggcac
     8041 gggcggtcat aaattcagca aaacactgaa tgagcataat aaagcggtgg cagaatggat
     8101 tcaaattgaa cgcaaacgca aaaatgaaca aaaatagtga gaacaatatg cgtggaaaat
     8161 ttattgtaat tgaaggctta gagggcgcag gcaaaacgac tgctcaccaa gtgattctca
     8221 aagagttgga acaagccggc attaatgacg tagtaatgac ccgagaaccc ggcggcaccc
     8281 cccttgccga aaagttacgc catttaatta aacacgaaac cgaagaagcc attaacgata
     8341 aggctgagct gctaatgctc tacgctgctc gtgttcaatt agtcgaaaat gtgatcaaac
     8401 ctgcccttgc cgaaggcaaa tgggttctgg gcgaccgcca cgatatgtct tcccaagcct
     8461 atcaaggcgg tggacgtaag ttggatcgtc acttactcga aaccttaaaa gaatcggtac
     8521 tcggcacatt cgagccagat ttaacgattt atctcgatct cgatccggtg attggtttag
     8581 aaagagccag aggcagaggc gaattagacc gcatcgaaca gcaaagcctt gacttcttct
     8641 accgcacacg agagcgttat ttagaattaa cccaacacaa cgataaagcc gtgatcatca
     8701 atgccgaaca acccatcgaa caggtgactg cggatattca acaagtaatt agaaactggc
     8761 tgaaataatg tacccttggc tcgaaagcac ttatcaacaa cttaccgatt catttttaca
     8821 gggtcgaggt catcacgcct tactgttcaa aacagatgtt ggtcttggta cagacacctt
     8881 aatctacaag gtggctgaat ggttgctttg cagccaaaaa ctccgccatc aaccttgtct
     8941 tgaatgcaaa agttgcctgc ttatgcaaag caacaaccac cccgatttcc acattcttga
     9001 gccgattgaa gacaaagata ttggtatcga ccaagtgcgt gagttgattt ataaactcca
     9061 gaatttcgcc caacaagggg gaaatacggt cgtttatatt cgaggagttg atcgtttaac
     9121 cgaggcaagc agtaatgccc tgctcaaaac cttggaagag ccacgtgaaa acgtctattt
     9181 cttgctcgaa gcaccgcttc aagcaacagt tttagctacc attcaaagcc gcagccaaac
     9241 ttgggtactt tacgccccac aagcctcaga agtaatggct tggctacaaa cacaatttcc
     9301 ggcaatctca accgacgact tagagattgc actccgcctt tgccactcac gcccactaat
     9361 ttgcaaaaat tttatcgaaa atgaccgctt gcaacagcga aaaacctttt tgcaaacctt
     9421 ctggcgtttc tacaaaagcc acgatgtctt cttgttattc caagccttcg cccaagaaaa
     9481 agagctgatt ttacagcaac tcgaatggct ggaaagtttc tttagcgatg ccctaaaagc
     9541 taaaatggca atcagccacg gttggctaaa cccggatctc caaaacggca ttctcccttt
     9601 tagccaacaa ctaacgaccc acggcttact taaagggcat caaattctcc aacaaaccca
     9661 gcgggattta accgaaatta acgccgtcaa tccggaattg atgttgctag attgcctgac
     9721 gaagttggtt ttggtgtttg agtaaaagcc ctctctcata gaaggtagct ttcaattagc
     9781 cgtaaaaaga ggcttctagc cctagaatat agtttataac ttgcaaaatt atctatcttt
     9841 gttattgtat gaataattca cacaaggaga atattatggc aagaacaacc agcgtgactt
     9901 taggcgaacc tttaaatgat tttgtaaatg aaatggtggc aagtgggcgt tatggctcta
     9961 ccagtgaagt agtgagaacc gctttacgaa tgcttgagga aaaagagcaa tacttagctc
    10021 agttgcgaca agccattgat gatggtgatg ctagtggtta tgcaaccgaa acgattgatg
    10081 aaatgatgtt gagagtaaag gcggaactta atgtataaac tttctgctca agcactccga
    10141 gatcaagaga aactttttcg ttacagtatc tctcaatttg gtgttcaaca aacagaatct
    10201 tattttgcag atctaaagca tattttgaat ttattagctg attttccaac aatgggaact
    10261 tctgcggaat atattcgtgg tggcttgcga atgcacacac atcaatctca tacaatttat
    10321 tatcgaataa aagcagatca tatatttatt gttcgcatct tacataacca aatgaatcat
    10381 aaacgctata tttagaaaaa tgaaagactt atttatcata gactcacatt gtcatctaga
    10441 ttctctagac tacgaaactc gtcacaaaaa cgtggacgaa gtaattgaaa atgccaaagc
    10501 ccgaggggtg catcatttta tctcggtttg cactacagtt gggcgttttg aagcaatgaa
    10561 aaacttaacc gcccatcgta atgacgtttc actttcttgt ggcgttcacc cgttgaatgt
    10621 ggaagatgaa ccttttgatt acgataaatt atttcagttc gctcaagatc cgaaagtggt
    10681 tgccattggg gaaactggtt tggattatca ctacacaccg gaaaccaaag cgttgcaaca
    10741 atcgcttttc gttcaacaaa ttgaaattgc aaacaaactc aataagccac tcattatcca
    10801 ttctcgttcg gcacgccaag atacgatgga tatgttggaa caaaataatg ccgaaaaatg
    10861 cggtggggtg ttgcattgct ttaccgaaga ttgggcaatg gcaaaacggg ctttagacat
    10921 cggcttttat atctcaattt cgggcattat tactttccgc aatgcagaag aactccgaga
    10981 agtcgtgcga aaagtgccgt tagatcgctt attggttgaa acagattccc cttatcttgc
    11041 cccgattcct tatcgtggta agccgaacca gccggcgtat gtgcgggaaa cttgcgaata
    11101 tgtggcgaca ttaaaaggcg tttcaactga agaattagct cgcattacaa ccgaaaatgt
    11161 gcaacactta ttcaacatta aattataagg aactaatatg agagcaatac ttaagtattt
    11221 tctgattctt gtctcactat cattttttat tgtattaggc ggtgcagtta attatgcaat
    11281 gccaagttat gaagataccg tagtaacagg tatggaagta cgccgaatgg ataaagatgg
    11341 tattatcagc aaagcgaatc ctgctgatgg cgaggtgcgc gatgtttatt tcttattcac
    11401 ggaagatcct gaaaccaaaa aggtaatggt ttatcgtaat gaagataccg gctgggggtt
    11461 gcctccttat ttcaaattcg gatctgccga cattcaagcc aaagctcaag cctacgctaa
    11521 cgaaaaacaa cgtgtgcaaa ttaaatatta cggctggcga atcaattggc taaatgaatt
    11581 tagaaatatt gtttcaatca agccattaga agaagcagaa acagtcagca aaccaattat
    11641 gagctatgta ctttatgccg tattggcgtt cttgttcttc ctctcagtac aatttattcg
    11701 tggcattttt aaagattaat gccgattaac ctttgacaaa cggttcgatt ttgtaaaaaa
    11761 ttgaaccgtt ttctttttta tcaacaatat caaatgacgt actattttat tgccgatctc
    11821 cacttaaatg aaaatcagcc tgaaattacc cacctctttt tgcaatttat gcaagaaaaa
    11881 gcactacttg cagaagcggt ttatatctta ggtgatctgt ttgatttctg gattggcgat
    11941 gatgagcagt ctgaactcat ttcaaccgtt aaacaggcaa tcaaaaccct cactacaagc
    12001 ggtacaaaat gctattttat ttgcggaaat cgtgattttt taattggcaa acgattcagc
    12061 gaacaaacgg gtatggttat tttgcctgat tatcacgtta tagatttatt cggcacatct
    12121 accttgcttt gccacggcga tacgctttgt actgatgatg tgaaatatca acaatttcgt
    12181 aaaaaagtcc accaaaaatg gcgacaagct atctttttga tgtttccgct ttcttggcga
    12241 attcgtattg cggagaaaat tcgatccaaa agcaaacaag aaaagcgtga taaatccgct
    12301 gaaattatgg acgtaaacgc tgaagttacc gctcaaattg tcgcaaaata caatacaact
    12361 caacttatac acgggcatac gcatcgacaa gcaatccatc agcataatgg atttacccgc
    12421 attgtattag gcgactggaa agaaaattac gcctctattt taagcgtaac cgagcaagga
    12481 gctgaattta tatgattaat gtgattattc cttgctataa cgccgaaaac acccttgttc
    12541 gtgcagtgca aagtgtatta aatcagtctg gattaaatct attgtggctt attgatgatg
    12601 cttcaacgga taatacgctt gccttagcta aacatttaca agcccaagtg cctgataaaa
    12661 ttagagtgga acaaatgcct caaaatggcg gtgttgccaa agcacgcaac tggggagcat
    12721 tacaaagtga aacagattta attgcctttt tagatgctga tgatgcttat gaaaacgggg
    12781 cattgcaagt tgctgaaaag atttttgagt ttaaaccgga aaccggcttg gttcggttag
    12841 cattaaaacc ggtaacttag atcaacgtta cagctctcac cctaattttg agttagcttg
    12901 gcaatatatg caaatgactg gtgcaggcaa cactgtattt cggcgtagtt tcttcctcgc
    12961 ttgtggcggc tttccacaac atcagctctt ccaagaatta ggtggagaag atggtgcttt
    13021 aggcatagcc actacacaaa ttagctctgt agctactgca tttaatgatg tcggtgtttt
    13081 acattactgc cgagagggta tgcacgcaga aagattatta aatgcgattt tattcaacga
    13141 aaaagatccc aatgttactg aagaaaaagt taaacaagcc aacctaatta ccgaaaatat
    13201 tgttaataat attagaaatc tacaagactg tcttaatagt aaagggattg gtattaagcc
    13261 ttatacgctt gaatggagct aaaaaagcat ttgcagattt tccctaaaaa aa
//
LOCUS       DANLXF010000056        13096 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.056, whole genome shotgun sequence.
ACCESSION   DANLXF010000056 DANLXF010000000
VERSION     DANLXF010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13096
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.056"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(64..1413)
                     /locus_tag="PXD66_001001"
     CDS             complement(64..1413)
                     /locus_tag="PXD66_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDL6319312.1"
                     /translation="MLLFIMFAAIILLLVLIMKFKVHAFVALIIVSLLTALAAGIPVD
                     KILPTLLNGFGSTLASVALLVGLGAMIGRLLEITGGAKVLADTLINKFGKERAPFALG
                     VASLLFGFPIFFDAGLVVMLPIIFSVAKQFGGSVLRYAFPSAGAFAVMHAFLPPHPGP
                     VASGDLLGVNMGLLVLVGLVCAIPTWYIGTYLFSQFISKRIHVDLPKAFLNGLSANEM
                     AVQNPPSFGKVLTVLVLPIILILFDTGLNTLSVAKVIDGSEMWVQSLRLIGKTPVALL
                     ITLIVAIMLLRDNRSFDQIEKICNNALGPICSIVLVTGAGGMFGGVLRASGIGDVLSE
                     MMKDTGMPIVVAAFIIATVFRVAQGSATVALTTTAALIAPTVAAATDLSQFDLCFIVI
                     AIASGATVLSHVNDSGFWLMSRFLEMDEKTTLKTWTMLETAIGLTGFACALIGSWLL"
     gene            1600..2115
                     /locus_tag="PXD66_001002"
     CDS             1600..2115
                     /locus_tag="PXD66_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDL6319313.1"
                     /translation="MANGKAFILMGVSSTGKTSVGTAVAQRLGIKLIDGDDLHPRANI
                     LKMASGTPLNDADRTPWLERINDAVFSFEQKSEKGIIVCSALKKKYRDQIRQGNADVT
                     FLFLHGSFELVLARMQQRKGHFMKPEMLQSQFNTLEIPQADEPDVIFIDIDGSFDEVV
                     ERCVKAVEPLL"
     gene            complement(2169..3167)
                     /gene="gntR"
                     /locus_tag="PXD66_001003"
     CDS             complement(2169..3167)
                     /gene="gntR"
                     /locus_tag="PXD66_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HDL6319314.1"
                     /translation="MRKPKRPTLQDIATHLGITKMTVSRFLRDPNTVAKETGERIAQA
                     IELFGYIPNRAPDILSNAKSRAIGVLVPSLTNQVFADVIKGIEQITDQASYQTMLAHY
                     GYSEQKEEQRIESLLSYNIDGIILSENHHSPRTLKMLEVANIPVIEIMESGECGIQQA
                     VGFDNISAAQAMVETMIHRGCRQVVYFGARMDKRSQLKMQGYEQAMLKHGLSPHSITT
                     AEHSSFTLGASQLKQALQHIPQLDGIFCTNDDLAIGALFECQRLGIHVPQQIKIAGFH
                     GHDVGQSLTPQLASVITPRLEIGRVAAQELLNRIHGVPQQSSIINLGYQIHLGESV"
     gene            complement(3260..4420)
                     /locus_tag="PXD66_001004"
     CDS             complement(3260..4420)
                     /locus_tag="PXD66_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HDL6319315.1"
                     /translation="MIEQSYKIALLFNANKIYDRGVMQGIGQFIQASQCTWDIFVEDD
                     FVYHKNSIESLSIDGIIADFDDLETVELLKDIDVPIVAVGGSYQNPDYYPDLPYVATD
                     NYALVEAAFLHLKQKGINQFAFYGYPAETEKHWAIERKNAFIQLMEKYEHHPNCYIGD
                     QFHSDNWVELQAKLGEWLQTLPAHTGIIAVTDARARHLLQTCEHLKIAVPEQLCVIGI
                     DNEELIQYLSRVSLSSVTQGTSQIGYHAAKLLYRKLNNLPVSTKPILIPPVKVEERRS
                     TDYRSLQDPMVIQAMHFIRHRACQGIKVEQVLDHLKISRSNLEQRFKQEMNKTIHQVI
                     HEEKLARATYMLKFTEISTQEIANVCGYPSLQYFYAVFKKEFGKTPKEFRQE"
     gene            complement(4543..4929)
                     /gene="rplQ"
                     /locus_tag="PXD66_001005"
     CDS             complement(4543..4929)
                     /gene="rplQ"
                     /locus_tag="PXD66_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDL6319316.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMASALVEHEIIKTTLPKAKEL
                     RRVVEPLITLAKADSVANRRLAFARTRNVDTVAKLFNELGPRFAQRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRPEVATEAAAE"
     gene            complement(4965..5954)
                     /gene="rpoA"
                     /locus_tag="PXD66_001006"
     CDS             complement(4965..5954)
                     /gene="rpoA"
                     /locus_tag="PXD66_001006"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDL6319317.1"
                     /translation="MQGSVTEFLKPHLVNIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSSKEGVQEDILEVLLNLKGLAVKVQNKDDIILTL
                     NKSGIGPVTAADITHDGDVEIVNPDHVICHLTDKNASINMRIRVQRGRGYVPASARLH
                     TQNDDRPIGRLLVDARFSPVDRIAYNVEAARVEQRTDLDKLVIEMETNGTLDPEEAIR
                     RAATILAEQLAAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAETIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIAED"
     gene            complement(5985..6611)
                     /gene="rpsD"
                     /locus_tag="PXD66_001007"
     CDS             complement(5985..6611)
                     /gene="rpsD"
                     /locus_tag="PXD66_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDL6319318.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIESKCRNRLDVAPGQHG
                     ARKPRLSDYGSQLREKQKVRRIYGILERQFRNYYTEANRLKGNTGENLLVLLEGRLDN
                     VVYRMGFAATRAEARQLVSHKSIVVNGRVVNIPSFQVSVDDVVAVREKSKKQARIKAS
                     LELATQREKPTWLEVDATKMEGVFKRRPERSDLSADINEHLIVELYSK"
     gene            complement(6640..7029)
                     /gene="rpsK"
                     /locus_tag="PXD66_001008"
     CDS             complement(6640..7029)
                     /gene="rpsK"
                     /locus_tag="PXD66_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDL6319319.1"
                     /translation="MAKTPVRARKRVKKQIADGVAHIHASFNNTIVTITDRQGNALAW
                     ATAGGSGFRGSRKSTPFAAQVAAERCAEMVKEFGLKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            complement(7046..7402)
                     /gene="rpsM"
                     /locus_tag="PXD66_001009"
     CDS             complement(7046..7402)
                     /gene="rpsM"
                     /locus_tag="PXD66_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDL6319320.1"
                     /translation="MARIAGINIPDQKHTVIALTAIYGIGKTRAKAICAATGIAEDVK
                     IRELSEEQIEKLREEVGKFTVEGDLRREVTLSIKRLLDLGCYRGLRHRRSLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            complement(7540..7653)
                     /gene="rpmJ"
                     /locus_tag="PXD66_001010"
     CDS             complement(7540..7653)
                     /gene="rpmJ"
                     /locus_tag="PXD66_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDL6319321.1"
                     /translation="MKVRASVKRMCRNCKVIKREGVVRVICSDPKHKQRQG"
     gene            complement(7678..8997)
                     /gene="secY"
                     /locus_tag="PXD66_001011"
     CDS             complement(7678..8997)
                     /gene="secY"
                     /locus_tag="PXD66_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDL6319322.1"
                     /translation="MAKQPGYQGSTQKGTGELKSRLLFVLGALIVFRIGSFIPVPGID
                     ASVLSELIRQQQGTIIDMFNMFSGGALSRASIFALGIMPYISASIIIQLLTAIHPPLA
                     ELKKEGEAGRRKISKYTRYGTLLLAFIQSIGISLALPNMVHGLVPNPSILFYVTSVVS
                     LVTGTMFLMWLGEQITERGIGNGISLIIFTGIVADLPATIGQTIEQARQGELSFIVLL
                     LVAIIAFAVTYFVVFVERGQRRIVVNYASRQQGRMMAPARTSHLPLKVNMAGVIPAIF
                     ASSIILFPASITQWFGQSEGFEWLFNLSQLLQPGQPLYVVLFTMAVIFFAFFYTGMQY
                     NPRDTADNLKKSGAFVPGYRPGEQTSRYIDKVMTRLTLIGALYIAFVCLVPYIITSLW
                     KVPFQLGGTSLLIVVVVIMDFIAQVQSHLMSQQYDSVLKKANLKGLN"
     gene            complement(9000..9434)
                     /gene="rplO"
                     /locus_tag="PXD66_001012"
     CDS             complement(9000..9434)
                     /gene="rplO"
                     /locus_tag="PXD66_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDL6319323.1"
                     /translation="MRLNSLSPAEGAKHSAKRLGRGIGSGLGKTGGRGHKGQKSRTGG
                     GVRRGFEGGQMPLYRRLPKFGFTSLKSFHVAEIRLNDLAKVDGNEVTLETLKAANIIT
                     KDILSAKVILAGKIERAVVVKGLRVTKGAKAAIEAAGGSIEE"
     gene            complement(9439..9615)
                     /gene="rpmD"
                     /locus_tag="PXD66_001013"
     CDS             complement(9439..9615)
                     /gene="rpmD"
                     /locus_tag="PXD66_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDL6319324.1"
                     /translation="MAKIKVTQTRSSIARLPKHKATLKGLGLRHIRHTVELEDTPAVR
                     GMINQVSYMVKVEE"
     gene            complement(9622..10122)
                     /gene="rpsE"
                     /locus_tag="PXD66_001014"
     CDS             complement(9622..10122)
                     /gene="rpsE"
                     /locus_tag="PXD66_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDL6319325.1"
                     /translation="MSNIEKQAGELQEKLIAVNRVSKTVKGGRIMSFTALTVVGDGNG
                     RVGFGHGKAREVPAAIQKAMEKARRNMINVALNEGTLQHPVKGSHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVRNVLSKAYGSTNPINVVRATIDALENMKSPEMVAAKRGKT
                     VEEILG"
     gene            complement(10137..10490)
                     /gene="rplR"
                     /locus_tag="PXD66_001015"
     CDS             complement(10137..10490)
                     /gene="rplR"
                     /locus_tag="PXD66_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDL6319326.1"
                     /translation="MDKKVARIRRASRARHLMREQGATRLVVHRTPRHIYAQVIAPNG
                     SEVLAAASTVEKVIKEQVKYTGNKEAAAVVGKIVAERAIAKGIKAVAFDRSGFKYHGR
                     VQSLADAAREAGLQF"
     gene            complement(10505..11038)
                     /gene="rplF"
                     /locus_tag="PXD66_001016"
     CDS             complement(10505..11038)
                     /gene="rplF"
                     /locus_tag="PXD66_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDL6319327.1"
                     /translation="MSRVAKAPVNIPAGVEVKLNGQLLTVKGKNGELSREIHNAVEVK
                     QEADALTFAPREGIANADAQAGTARALVNNMVIGVTEGFTKKLQLVGVGYRAQMKGNA
                     VALSLGFSHPVEHTLPAGVTGECPSQTEIILKSADKQLIGQVAADIRAYRKPEPYKGK
                     GVRYSDEVVRTKEAKKK"
     gene            complement(11055..11447)
                     /gene="rpsH"
                     /locus_tag="PXD66_001017"
     CDS             complement(11055..11447)
                     /gene="rpsH"
                     /locus_tag="PXD66_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDL6319328.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKVAISMPSSKLKVAIANVLAEEG
                     YIESVKVVEGVKPELEITLKYFQNKPVVESIQRVSRPGLRIYKRKDELPKVMGGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            complement(11484..11789)
                     /gene="rpsN"
                     /locus_tag="PXD66_001018"
     CDS             complement(11484..11789)
                     /gene="rpsN"
                     /locus_tag="PXD66_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDL6319329.1"
                     /translation="MAKQSMIARDVKRAKLADKFYAKREELKKIISDVNASDEDRWAA
                     VLKLQTLPRDSSPIRQRNRCRQTGRPHGVLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            complement(11801..12340)
                     /gene="rplE"
                     /locus_tag="PXD66_001019"
     CDS             complement(11801..12340)
                     /gene="rplE"
                     /locus_tag="PXD66_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDL6319330.1"
                     /translation="MAKLHDYYRDTVVNELKAKFNYSSVMQVPRIEKITLNMGVGEAL
                     TDKKLLDNAVADLAAISGQKPLVTKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLNAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTSA
                     KTDEEGQALLAAFNFPFRK"
     gene            complement(12358..12669)
                     /gene="rplX"
                     /locus_tag="PXD66_001020"
     CDS             complement(12358..12669)
                     /gene="rplX"
                     /locus_tag="PXD66_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDL6319331.1"
                     /translation="MAAKIRQNDEVIVLTGKDKGKRGKVTKVLPNGKVFVEGINIITK
                     HEKPVPALGKAGGLVKKEAAIDVSNVAIFNPETNKADRVGFRFEDGKKVRFFKSNEKT
                     I"
     gene            complement(12680..13051)
                     /gene="rplN"
                     /locus_tag="PXD66_001021"
     CDS             complement(12680..13051)
                     /gene="rplN"
                     /locus_tag="PXD66_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDL6319332.1"
                     /translation="MIQEQTMLDVADNSGARSVMCIKVLGGSHRRYAAIGDIIKVTVK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGAVIRFDGNACVILNNNTEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
BASE COUNT         3739 a         2930 c         2583 g         3844 t
ORIGIN      
        1 ggtcgattct ctgccaaatt ttgcaaattt tagaagaaaa cagaccgctt gtcagggttg
       61 gaattagagt aaccagctgc caatcaatgc acaggcaaaa ccggttaaac cgatggcagt
      121 ttcaagcatt gtccacgttt tgagggtagt tttttcgtcc atttcaagga aacggctcat
      181 cagccagaaa ccggagtcat tgacgtggga aagcaccgtc gcaccggagg cgatagcaat
      241 cacgataaaa cagaggtcga actggcttaa atcagtcgct gccgccacag ttggcgcaat
      301 caacgctgct gtggtggtta aagcaacggt tgccgagcct tgtgcgacac ggaataccgt
      361 agcaataata aaggcggcaa ccacaatcgg cattccggtg tctttcatca tttccgataa
      421 tacatcgcca atcccgctgg cacgcaacac gccaccaaac attccgcctg caccggttac
      481 taagacaatc gagcagattg ggccaagggc attgttacag attttttcga tttggtcgaa
      541 actgcgatta tcccgcaaca gcataatcgc cacaattaag gtaatcagta aagcaacagg
      601 ggttttgccg attaagcgaa ggctctgcac ccacatttca ctgccatcaa tcactttagc
      661 aacggagagg gtattcaagc cggtatcaaa caaaatcagg ataatcggca gcaccaacac
      721 cgtgagtacc ttgccaaaac ttggcgggtt ttgtactgcc atttcgtttg ccgataagcc
      781 gtttaaaaac gctttcggca aatcaacgtg aatgcgtttg ctgataaatt ggctgaacag
      841 gtaagtgccg atataccacg ttgggattgc acaaaccaag cccaccaaga ctaacagccc
      901 catattcaca ccaagcagat cgccggaggc aaccgggccg gggtgcggag gtagaaaggc
      961 gtgcattacc gcaaacgcac cggcagacgg aaacgcatag cgcaacaccg aaccgccgaa
     1021 ctgttttgcc acgctaaaaa taatcggtaa catcaccacc aagccggcat caaagaaaat
     1081 cgggaagccg aataacagcg aggcaacgcc caacgcaaaa ggcgcacgtt ctttgccgaa
     1141 tttattgata agcgtatccg caagcacttt cgccccgccg gtgatttcaa gcaagcgacc
     1201 aatcatcgcc ccaagcccca ccagtaacgc cactgatgcc agcgtactac caaaaccatt
     1261 caacaatgtt ggcaggattt tatctaccgg aataccggca gctaatgcag tgagcaggct
     1321 cacaataatc aatgccacaa aagcgtggac tttaaatttc ataatcagca ccagcaataa
     1381 aatgatggca gcaaacataa tgaacagtaa cataggatct cctaaggttg aattgttatc
     1441 ggtaacataa ccaaaaagca gtttagagta aacggttgca gggcttttat tttaaaaatg
     1501 tgatctactt caagttttct ggaaatagca gatgattttt cagcataacc tgttacatta
     1561 acggctaagt taacgttacc agtaacacaa ggaaattcaa tggcaaacgg caaagcattt
     1621 attttgatgg gcgtgtccag caccggcaaa accagcgtgg gaacggcagt cgcacagcgg
     1681 ttgggaataa aattgattga tggggacgac ttacaccctc gtgccaatat actgaaaatg
     1741 gcaagtggca cgccactcaa tgatgcagat cgcacaccgt ggttggagcg gattaacgat
     1801 gcagtgttca gttttgaaca aaaaagcgaa aaagggatta tcgtctgctc agcattgaag
     1861 aaaaagtacc gagaccagat ccgacaaggc aatgccgatg tgacttttct attcttgcac
     1921 ggctcgtttg aactggtgct ggctcgaatg caacaacgca aagggcattt tatgaaaccg
     1981 gaaatgcttc aaagccaatt taatacgctg gaaattccgc aggcagacga accagatgtg
     2041 atatttattg atattgacgg cagttttgac gaggtggtgg agcgttgcgt gaaagcagtt
     2101 gagccgttgc tttaatcaca agtggtggat tttgcggatt tttttgcaaa tttaaccgct
     2161 tgtccgggct acacactttc ccccaaatgg atctgatagc ccaagttaat tatggagctt
     2221 tgttgtggta cgccgtgaat acggttgagc agctcttggg cggcaactct gcctatttca
     2281 aggcgaggtg tgatgacgct ggcaagctgt ggcgtgaggg attgcccgac atcgtgtccg
     2341 tgaaagccgg cgattttaat ctgctgcggc acatgaatgc ctaaccgttg gcactcaaat
     2401 aatgccccga ttgccaaatc gtcattggtg caaaaaatgc cgtctagctg cggaatgtgt
     2461 tgcagggctt gttttagctg gcttgcgcca agcgtgaaag aagaatgctc tgcagtagta
     2521 atgctgtgtg ggcttaagcc gtgtttgagc attgcctgtt cgtagccctg cattttcagc
     2581 tgagagcgtt tatccattct cgccccgaaa tacaccacct gtcggcagcc tcgatgaatc
     2641 attgtctcga ccattgcttg agccgcagaa atattgtcaa atccgaccgc ttgttgaatg
     2701 ccacattcgc cgctttccat tatctcaatc accggaatat tcgccacttc cagcattttc
     2761 aaggtgcggg gcgagtggtg attttccgat aagataatgc cgtcaatatt gtacgaaagc
     2821 agcgactcaa tccgctgctc ttccttttgt tcgctgtaac catagtgggc gagcatggtt
     2881 tgatagctgg cttggtcggt aatctgctca atgcctttaa tcacatcggc aaacacttga
     2941 ttggtaagcg aaggaaccag cacgccaatt gcacggcttt tggcattcga gagaatatcc
     3001 ggtgcacggt ttgggatata gccaaacagc tcaatcgctt gtgcaatccg ctcgccggtc
     3061 tccttggcaa cggtgtttgg atcccgcaaa aagcgactga ccgtcatttt ggtaatgccg
     3121 agatgggtag cgatgtcttg caaagttggg cgtttcggtt ttctcattgg aatagataac
     3181 gaaaaaattt aatttcgctg attataactg aagataaggc aataattgca aaatattcgg
     3241 tgaaattgac cgcttgttac tactcttgcc tgaactcttt cggggtttta ccaaactctt
     3301 ttttaaacac ggcatagaaa tattgtaaag agggataacc gcacacattg gcaatttctt
     3361 gggtcgaaat ctcggtaaat ttgagcatat atgttgctct tgctaatttt tcctcgtgaa
     3421 tcacctggtg aattgtctta ttcatttcct gcttaaaacg ctgttcaagg ttagagcggg
     3481 aaattttaag atgatccagt acttgctcta ctttaatgcc ttggcaagct cggtgacgaa
     3541 taaagtgcat agcttggatt accattgggt cttgcaaaga acgataatcg gtcgaacggc
     3601 gttcttccac ttttaccggc gggattaaaa tcggcttggt tgaaaccggt agattattta
     3661 attttcgata taataacttc gcagcgtgat agccgatttg tgatgttcct tgtgtgactg
     3721 aagaaagaga aacccgagag aggtattgaa tcaactcttc gttatcaatg ccgatcacgc
     3781 aaagttgttc aggtacagcg atttttaaat gctcacaggt ttgaagcagg tgtcttgccc
     3841 ttgcgtcagt aacggcaata atgcccgtgt gggctggtaa ggtttgtaac cactcgccta
     3901 atttggcttg taattcaacc caattatctg aatgaaattg atcaccaata taacaatttg
     3961 ggtgatgttc atatttttcc atcaactgaa taaaggcatt ttttcgctct atcgcccaat
     4021 gtttttccgt ttctgcagga tagccgtaaa acgcaaattg gttgattcct ttttgtttta
     4081 ggtgtaaaaa tgctgcctca actaaggcgt aattgtcggt agcaacataa ggaaggtcag
     4141 ggtaatagtc agggttttgg taagaaccgc ctactgctac aatggggacg tcaatatctt
     4201 taagtagctc aactgtttcc aaatcgtcaa aatcggcaat aatgccgtca atcgacaggc
     4261 tttcgatact atttttatga tagacaaaat catcttccac aaaaatatcc caagtacatt
     4321 gtgatgcttg aataaactgc ccaatgccct gcattactcc tctgtcataa attttgttag
     4381 cattaaaaag taaggcaatt ttataagatt gttctatcat tacttcctcc agtacccact
     4441 ttcaaaagta actataaaat aaaattattt tttgatcaaa acgaataaaa gaataaaacc
     4501 ccggtatttc taccagggtt ttaagctcaa ttttgattaa gattattccg cagctgcttc
     4561 agttgcaact tctgggcgat ccactaactc aatgtaagcc attggagcat tatcacctgc
     4621 acggaaacca cattttaaaa tgcgagtgta accacccgca cgttgtgcaa aacgtggacc
     4681 taattcattg aataatttag caactgtatc aacgttgcgt gtacgggcaa aagctaaacg
     4741 gcgatttgca acgctgtctg ctttagctaa agtaattaac ggctcaacta cacggcgtaa
     4801 ctctttcgct tttggtaaag ttgtcttgat gatttcgtgt tcaacaagcg cacttgccat
     4861 attacggaac atcgcttggc gatggctgct gttacggttt aattgacgtc cactcttacg
     4921 atggcgcata acctattcct tcttagaaaa tctataccga aaaactagtc ttctgcaata
     4981 cttgcaggtg gccagttttc aaggcgcata ccgagtgata aaccacgaga agcaagaaca
     5041 tccttaatct ctgtaagaga ttttttaccc aagttcgggg ttttaagaag ttcaacttct
     5101 gtacgttgta ctaaatcacc gatatagtga attgtctctg ctttcaaaca gttagcagaa
     5161 cgaactgtca gctctaggtc atctactgga cgaagaagaa taggatcaaa ttccggtttt
     5221 tcttctttca cttcaggttg acgaacatca cgcaaatcaa cgaatgcagc aagttgttct
     5281 gctaaaatcg ttgcagcacg acggatagct tcttcaggat ctaacgttcc gttagtttcc
     5341 atctcaataa caagtttatc taagtctgta cgttgttcaa cacgagcagc ttcgacattg
     5401 taagcaatgc gatctacagg tgaaaaacgt gcatctacta ataaacgacc gatagggcgg
     5461 tcatcgtttt gagtatgaag acgtgctgat gcaggaacat aacctcgacc acgttgaaca
     5521 cgaatgcgca tattaattga tgcattctta tctgttaaat gacagattac gtgatcagga
     5581 ttcacaattt ctacatcgcc atcgtgtgtg atgtcggctg cagttacagg gccaattcca
     5641 gatttattta gtgtcaaaat aatatcatct ttattttgca cttttaccgc tagaccttta
     5701 aggtttaata atacctcaag aatatcttcc tgaacgcctt ctttgctgct gtactcatgc
     5761 agtacaccat caatttcaac ttcagtaact gcacaacccg gcatagatga aagtaaaatg
     5821 cggcgaagtg cgttacctaa tgtatggcca aaaccacgct ctaacggctc taatgtcact
     5881 ttagcatgag ttgaactaac ttgttcaata tttactaagt gcggctttaa aaattctgtc
     5941 acagaaccct gcattttttc ctctctttgc ggttaagcta actattattt agagtaaagc
     6001 tcaacgatca gatgttcatt aatatctgct gatagatcag aacgctctgg acgacgttta
     6061 aatacgcctt ccattttagt tgcatcaact tctaaccaag ttggtttttc acgttgagtt
     6121 gctaattcta atgatgcttt aatacgtgct tgttttttcg atttttcacg aacagcaaca
     6181 acatcatcaa cagaaacttg gaatgaagga atgtttacta cacgaccgtt tactacgatt
     6241 gatttatggc taacaagctg acgtgcttct gcacgtgttg ctgcaaaacc catacgataa
     6301 actacgttat ctaaacgacc ttccaataat actagtaagt tttcaccggt attacctttt
     6361 aagcggttcg cttctgtata ataattacgg aattgacgct ctaagatacc ataaatacgg
     6421 cgaacttttt gtttttcacg taattgacta ccatagtcag ataaacgtgg cttgcgagca
     6481 ccatgttgac caggcgctac atcaagtcta tttctacatt ttgattcaat cgcacgaacg
     6541 cctgatttta agaataaatc agtaccttca cgacggctta gcttgagctt aggacccaaa
     6601 tatcttgcca ttttctttct ccaattcttt taacgtcaat taaacacgac gttttttcgg
     6661 tggacgacaa ccgttatgtg gaatcggagt cacatcagta atattcgtga tacggaaacc
     6721 tgctgcattt aatgcacgga ttgttgactc acgacctgga cccggtcctt taaccataac
     6781 ttccaaattc tttaaaccaa actctttaac catttctgca caacgctctg cagcaacttg
     6841 agcagcgaat ggagtcgatt tacgagaacc acggaaacct gaaccacctg cagttgccca
     6901 agctagagca ttaccttgac ggtcagtaat agtaacgatt gtgttattga aagatgcgtg
     6961 gatatgagct acgccatctg caatctgttt ttttacgcgc ttacgtgcgc gaactggtgt
     7021 tttagccatt atttatctac tccgactatt ttttgatcgg tttgcgtgga cctttacggg
     7081 tacgcgcatt agtcttagta cgttgaccac gtaccggtaa actacgacga tgacgtaaac
     7141 cacggtagca acctaagtct aaaagacgtt tgatgcttaa agttacttca cgacgtaaat
     7201 ccccttcgac agtaaattta ccaacttctt cacgcagttt ttcaatctgc tcttcagaca
     7261 attctctgat ctttacatct tcagcaatac ccgttgcagc acagatggct tttgcacgag
     7321 ttttaccgat accgtaaatt gcagttaatg cgattacagt gtgtttttga tcaggaatgt
     7381 taatgcctgc aatacgggcc actatgcact cctattattt aagtttattg atatactgat
     7441 cttgaaaagc ccgtttcagg atactcaaac agtatatcag gacgaatgag ctgagcagta
     7501 taactgctca gcgggttctt tgcaagaaat atgcgtaaat taaccttgac gttgtttgtg
     7561 tttaggatcg ctacaaatta cacgaaccac accttcacgt ttgattactt tacaattacg
     7621 gcacattctt tttactgaag cacgaacttt cattgcttat cctttattaa cggcctatta
     7681 atttagacct tttaagttag ctttcttcaa cacagaatca tattgttgag acattaaatg
     7741 actctgaacc tgtgcgatga aatccataat aacaaccact acaatcaata atgatgtacc
     7801 acctaattgg aatggcacct tccataacga cgtaatgatg taaggaacca aacaaacaaa
     7861 agcaatatat aacgcaccaa ttaaggttaa acgtgtcatt actttatcga tataacgaga
     7921 tgtttgttcg cctggtcggt aacctggtac aaacgcacct gatttcttaa gattatccgc
     7981 tgtatcacga ggattatact gcattcctgt ataaaagaaa gcaaagaaaa tcactgccat
     8041 tgtgaataat acaacataca gtggttgacc tggctgtaat agttgtgaaa ggttaaataa
     8101 ccactcaaat ccttcgcttt gaccaaacca ttgagtaatg cttgccggga ataaaataat
     8161 acttgaagca aaaattgccg gtattacccc tgccatattc acttttaatg gtaagtgaga
     8221 agttcttgca ggcgccatca ttcgaccttg ttggcgactt gcatagttta caactattct
     8281 tctttgtcca cgttcaacaa aaacaacaaa ataagttact gcaaatgcaa ttattgcaac
     8341 tagtagtaag acgataaatg ataactcgcc ttgacgggct tgttctattg tctgccctat
     8401 cgttgctgga agatctgcaa caatacctgt aaaaataatc aatgagatac cgtttccgat
     8461 accacgctca gtgatttgtt cgcctagcca cattaggaac attgttcctg tcactaagct
     8521 tactacagag gttacataga ataatatact tggattagga actaatccat gtaccatatt
     8581 cggtagggct agtgaaatac caatagattg aataaatgct aataacagtg tgccatagcg
     8641 tgtatattta ctgattttcc gacgacctgc ttcaccttct ttctttaatt cagctagagg
     8701 tgggtgaatc gctgtcaata attggataat aattgacgct gaaatataag gcataatacc
     8761 tagtgcaaat atagatgcac ggcttaaagc accaccagag aacatattaa acatatcaat
     8821 gatggtgcct tgctgttgtc gaattaattc ggataataca gaagcatcaa tgccaggaac
     8881 aggtataaaa gaaccgatac ggaaaacgat taacgcacct aaaacaaaca atagtcttga
     8941 tttaagctcg ccagtccctt tttgtgtact accttggtac cctggttgct ttgccatctt
     9001 tattcctcga tagaaccgcc agcagcttca atagcagctt tagcgccttt agttacacgt
     9061 aagcctttaa caactactgc tctttcaatt ttgcctgcta aaattacttt agcagataaa
     9121 atatctttag taatgatgtt tgctgctttt aatgtctcta aagttacttc gttgccatct
     9181 actttagcta agtcgtttaa acgaatttca gccacatgga atgattttaa tgaagtaaaa
     9241 ccaaattttg gcaaacgacg atataaaggc atttgaccac cctcgaaacc acgacgaaca
     9301 ccgccgcctg tacgagattt ttgaccttta tgaccgcgcc caccagtttt acctaaacca
     9361 gaaccaatac cacgacctag acgttttgca ctgtgcttag caccttcagc tggagaaaga
     9421 gaatttaaac gcattctatt actcctctac tttaaccata taagacactt gattgatcat
     9481 accacgcact gctggtgtat cttcaagttc tacagtatga cgaatatgac gaagacctaa
     9541 gccttttaac gttgctttat gtttcggtaa acgagcgata gagctacgag tttgagttac
     9601 tttaattttt gccatatcca attaccccaa aatttcttca actgttttgc cacgttttgc
     9661 agcaaccatt tcaggtgatt tcatgttctc aagtgcatcg attgttgcac gaacaacgtt
     9721 aattgggtta gttgaaccat acgctttaga aagaacgtta cgaacacccg caacttctaa
     9781 tactgcacgc attgcaccac cagcgatgat acctgtacct tcacttgctg gttgcataaa
     9841 tacgcgtgaa cctgtatgtg aacctttaac tggatgttgt aatgtacctt catttaaagc
     9901 tacattaatc atattgcgac gagctttttc catcgctttt tggattgctg ctggaacttc
     9961 acgtgcttta ccgtgaccaa aacctacacg accgttacca tcgcccacta ctgttaaagc
    10021 agtgaaactc ataatacgac caccttttac ggtttttgaa acacggttaa ccgcgattag
    10081 cttttcctgc agttcaccag cttgtttttc gatgtttgac atctcaaatt cctctattag
    10141 aactgtagac cagcttcacg agcagcatct gctaatgatt gcacacgacc gtggtattta
    10201 aaaccagaac ggtcaaaagc aaccgcttta atacctttgg caattgcacg ctcagcaacg
    10261 attttaccca ctactgctgc ggcttcttta ttgccggtat atttaacttg ctctttaatt
    10321 actttttcaa cagttgaagc tgctgctagt acttctgagc cattaggtgc aatcacctgc
    10381 gcatagatat ggcgaggtgt gcgatgcaca actaaacgtg ttgcaccttg ctctctcatt
    10441 aaatgacgtg cacggcttgc acgacggata cgagctactt tcttatccat agtgttacct
    10501 tactttactt tttcttcgct tcttttgtac gcactacttc atcagagtaa cgtacacctt
    10561 tacctttata aggttcaggt ttgcgatatg cacgaatatc tgctgccact tgaccaatta
    10621 actgtttgtc agcacttttc agaataattt ctgtttgtga tggacattca ccagttacgc
    10681 ctgctggcaa cgtgtgttct actgggtgag agaaacctaa acttaaagca acagcattgc
    10741 ctttcatttg tgctctataa ccaacaccca ctaattgcaa tttcttagta aagccttcag
    10801 taaccccgat taccatatta tttactagtg cacgagcagt acctgcttga gcatctgcat
    10861 tagcgatacc ttcacgtgga gcgaaagtta atgcatctgc ttcttgtttt acttcaactg
    10921 cattgtgaat ttcacgagat aactcgccgt ttttaccttt aactgttaat agctgaccgt
    10981 taagttttac ctcaacaccg gcaggaatat taacaggtgc ttttgcaaca cgagacattt
    11041 ttcctacctc tctattatgc tacgtaacag ataatttcac cgccgaggcc cgcttgacgc
    11101 gcagcacggt cggtcattac acctttagat gtagaaacaa ctgcgatacc taaaccaccc
    11161 attacttttg gtaattcgtc tttgcgttta taaatacgca gacccggacg gctcacacgt
    11221 tggatacttt ctacaaccgg tttgttttgg aaatatttta aagtaatttc cagttcaggt
    11281 ttaacacctt caacaacttt aacgctttcg atataacctt cttcagctaa aacatttgca
    11341 atagcaactt ttaacttaga tgaaggcata ctgattgcaa ctttgttcgc agcttgaccg
    11401 ttacgaatac gggtcagcat atctgcgatt ggatcttgca tgctcatgta tttactccca
    11461 tgattccaaa ttaaaagagg ttattaccag cttgccttct taagacccgg aatttcaccg
    11521 cgcatagcag cttcacgaac cttaatacgg cttaaaccaa acttacgaag tacgccatgt
    11581 ggacgaccag tttggcggca acgattacgc tgacggattg ggctagaatc acgaggaagt
    11641 gtttgtaatt ttaacactgc tgcccaacgg tcttcatcag atgcattcac atcagagatg
    11701 attttcttta attcttcacg ttttgcgtag aatttatcag ctaatttagc acgttttaca
    11761 tcgcgtgcga tcattgattg ttttgccacg ataacctacc ttatttacgg aatgggaaat
    11821 tgaaagcagc taataaagct tgaccttctt catcagtttt cgctgaggtg gtgatagtaa
    11881 tatctaaacc acgaacacga tctactttat cgtagtcgat ttcagggaaa atgatttgtt
    11941 cacgaacacc catactgtaa ttaccacgac catcgaatga cttagcgttt aaaccgcgga
    12001 agtcacgaat acgtggaaca gcgatagtaa tcaatctttc aaagaattcc cacatacgtt
    12061 caccacgtag ggttactttg caaccgattg gatacccttg acggatttta aagcctgcaa
    12121 cagatttgcg agctttagtt actaaaggtt tttgaccgct gattgctgct aaatccgcta
    12181 ctgcgttatc taacagtttt ttgtcggtca atgcttcacc cacacccatg ttaagggtaa
    12241 tcttttcgat tcgtgggact tgcatgacag atgagtagtt gaattttgct tttaattcat
    12301 taactactgt atctctgtag taatcatgca gtttcgccat cacattactc cagttaatta
    12361 aattgttttc tcgttagatt tgaagaaacg cacttttttg ccatcttcga atctaaatcc
    12421 tacacggtca gctttgttgg tttcagggtt gaagatcgca acgtttgaaa catcgattgc
    12481 agcttctttc ttcactaaac caccagcttt gcctaatgca ggaactggtt tttcgtgttt
    12541 agtgatgatg ttgataccct caacaaaaac tttaccattt ggtaacactt tagttacctt
    12601 accacgtttg cccttatctt taccggtaag aacaattact tcatcgtttt gacggatttt
    12661 tgcagccatt acattctcct tatagtactt ctggagctaa agagatgatt ttcataaact
    12721 tctcagaacg aagttcacga gtcaccggtc caaaaatacg agtaccgatt ggttgctcag
    12781 tgttattgtt taaaattaca caagcattgc catcgaaacg aataactgcg ccatctgggc
    12841 gacgaacacc cttcttggtg cgcacaacaa ctgcttttaa cacatcacct tttttcactt
    12901 taccgcgtgg aattgcttct tttacagtaa ctttaatgat atcgccaata gcagcgtaac
    12961 gacggtgcga tccacctaga accttgatac acattacgct acgagcccct gagttatcag
    13021 caacatccag catagtctgt tcttggatca tattagtgct ccgttaaaat aaaaaaacac
    13081 cctttcggga cgaacc
//
LOCUS       DANLXF010000057        12948 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.057, whole genome shotgun sequence.
ACCESSION   DANLXF010000057 DANLXF010000000
VERSION     DANLXF010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12948
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.057"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            68..1117
                     /gene="ansB"
                     /locus_tag="PXD66_001022"
     CDS             68..1117
                     /gene="ansB"
                     /locus_tag="PXD66_001022"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HDL6319333.1"
                     /translation="MKIKKLLLPTLLSIATMPIQAADLPNITILATGGTIAGSQKNTA
                     TSAYQAGQLNIETLIEAVPDIKKLANIKGEQIVKIGSQDMSDEVWLKLAKAINQQCPN
                     TDGFVITHGTDTMEETVYFLDMTVKCEKPVVLVGAMRPATEKSADGVLNLYNSVVVAK
                     DSKSSGRGVLVAMNDIVLGARDVTKTSTTSVQTFNSPNFGALGYIHNSKVDYERSPES
                     KHTVNTPFKVDELTELPKVGIIYAYSNMPTEPLQALLDASYQGIVVAGVGNGNMNAAN
                     LALLEEAAKKGITVVRSSRVPTGYTTRDAEIDDSKYGFIASGTLNPQKARVLLQLALT
                     QTNDIKTIQQYFEDF"
     gene            complement(1191..1835)
                     /locus_tag="PXD66_001023"
     CDS             complement(1191..1835)
                     /locus_tag="PXD66_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319334.1"
                     /translation="MRIWQKLASVIVVTLGLISNASAENFKLKYSSNYLMPAYINFNN
                     GGGQYNIQAKINVPLYNIVFSAKGTEKNEQFHMLSYRDTRNGKTYAMAEIDSKTVKYG
                     KVKEPLKEEALTMPTYDLFTTAFQLSYYDKLPSSFQTTNGKKLYPTPNVQLRKAQKEV
                     KVGGKTYQEITYKFRTSDKKDITVKKFEGEKFPRYIAYSRDGDNYELTFDSFVK"
     gene            complement(1836..2819)
                     /gene="cysB"
                     /locus_tag="PXD66_001024"
     CDS             complement(1836..2819)
                     /gene="cysB"
                     /locus_tag="PXD66_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HDL6319335.1"
                     /translation="MKLQQLRYIVEIVNQNLNITEAAEVLYTSQPGISKQVRLLESEL
                     GINIFERNGKHVKGLTPAGEKIVAIARELLVKAQSIKSVAEEQTRPNKGILRIATTNT
                     QARYMLPSVIEKFKARYPEVSLHLHQGSPNQIYDALVAGEVDLAITTEAQYLFDEAVL
                     LPCYWWNRSVIVKPEHPLAAFVNQSKNLTVEQLGRYDLITYTFGFTGQSDLDHAFNKE
                     GILPNIVFTATDADVIKTYVRLGLGVGIMASMAHTETDKDLVAINASHLFQPSMTQIA
                     FKRGTFLRNYMYDFINYFSPHLTRVQVEKAEQLRDNNAISRLFESISLEEK"
     gene            complement(2816..3499)
                     /gene="yjjG"
                     /locus_tag="PXD66_001025"
     CDS             complement(2816..3499)
                     /gene="yjjG"
                     /locus_tag="PXD66_001025"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HDL6319336.1"
                     /translation="MKYQWVLFDADETLFSFNSYLGLKAMLARYQIDFSEQDYQDFQA
                     VNKPLWVAYQNNEITAQDIQTRRFVKLSAQTGVDPLQLNQELMAEMAFVSQPLEGVMD
                     MLNALYGKVKMGIITNGFTDLQQKRLDNTQTSHFFDIVVISEQIGAAKPDRQVFDYAF
                     ALMDEFDRTKVLMVGDTLASDILGGNNAGIDTCWLNLLSKENDTDIKPTYEVHTMQQI
                     IAIVEGKSL"
     gene            complement(3575..4468)
                     /locus_tag="PXD66_001026"
     CDS             complement(3575..4468)
                     /locus_tag="PXD66_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HDL6319337.1"
                     /translation="MNKKLPPLNALKAFESAARHLNFTKAAEDLFVTQAAVSHQIKLL
                     EDFLGASLFHRRNRLLELTELGAQYFYEVQPLLEQIALATDKIRQQSSRQILTISVPQ
                     TFGMHWLVPRLADFHEKYPEVEVRIKGVDQDEGLLGKETDVAIYYGKGDWQGLESIRL
                     TESRIVILASPEYLSQNTIHLPADLAGKHLLHSSTHNKWKRMVEHLHIEDIVDAETGS
                     IFSHTFMVLQAAMHQQGIAIANQLLAQHEIEKGTLIEPFPTTLFDEKSFYVVYPSQSA
                     QVQKVQFFVEWIQQKMKECCA"
     gene            4914..5951
                     /gene="rsgA"
                     /locus_tag="PXD66_001027"
     CDS             4914..5951
                     /gene="rsgA"
                     /locus_tag="PXD66_001027"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HDL6319338.1"
                     /translation="MSKPKLTHNQQRRIQSNHHKRINKKEIEWQDEMLGEMQQGIVVT
                     RHVKHADVETAEGQIFRCNIRRTLKNVVVGDQVSWRKGSEQLQGISGVIEAVFPRKNE
                     LTRPDYYDGIKVMASNIDQIIIVSAVLPTLSLNIIDRYLVICETANIPALIVLNKIDL
                     LNESERQAVKEQLKIYEEIGYETLCLSADTGENMQALHQYLAKGTSIFVGQSGVGKSS
                     LINQLLPEVNALTGSVSDNSGLGQHTTTASRLYHLPQGGQLIDSPGIREFGLWHLEPE
                     QITLGYREFQSVLGTCKFRDCKHKTDPGCALRQEVENGEISAIRFENYHRLIESLSET
                     KSQRHFRKEEV"
     gene            5948..6547
                     /gene="rnhB"
                     /locus_tag="PXD66_001028"
     CDS             5948..6547
                     /gene="rnhB"
                     /locus_tag="PXD66_001028"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDL6319339.1"
                     /translation="MMNEFIYPNARLIAGVDEVGRGPLVGAVVTAAVILDPNNPIQGL
                     TDSKKLSEKKRQALAEEIKAKALSWSLGRAEPTEIDELNILNATMLAMQRAVAGLHIQ
                     PDFVLIDGNRPPKLPMPAQAVVKGDSLVAEISAASIIAKVARDQEMDELDQQFPQYGF
                     AQHKGYPTKLHFEKLAEFGATPYHRKSFAPVRKVLGLID"
     gene            6560..6919
                     /locus_tag="PXD66_001029"
     CDS             6560..6919
                     /locus_tag="PXD66_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HDL6319340.1"
                     /translation="MTTEYQKTIFTKIINKEIPANIVYQDELVTAFRDIAPQAPTHIL
                     IIPNKFIPTVNHVSNEDELALGRLFTVAAKLAQEEGIAEEGYRLVMNCNKNGGQEVFH
                     IHMHLLGGQKLGPLVAK"
     gene            6922..7293
                     /locus_tag="PXD66_001030"
     CDS             6922..7293
                     /locus_tag="PXD66_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HDL6319341.1"
                     /translation="MRISYFAFAIFSIFLTACGSKPVSYLPTGSEPIVNIESDIAEKI
                     ELETNSKQFSTTNLTEFPLNLTYKLFWYNKDGVTQSHNGISDSIGWQHLWLQPKQKQT
                     IALIKPTEESHNYRLYLRGVK"
     gene            complement(7328..7957)
                     /locus_tag="PXD66_001031"
     CDS             complement(7328..7957)
                     /locus_tag="PXD66_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL6319342.1"
                     /translation="MFNLQIIPVSPFQQNCTVIWDENKNAAIIDGGDNAEGIIQFVES
                     QQLKVQKLLITHGHLDHIMAVEKLREHFQVEVFGSHLADKPLFERLPEMCQQFGLPPV
                     NGFLPDHWLEEGDRVEVGELSYTVRHLPGHAPGHIGFFDFDNRIAFSGDVLFQNSIGR
                     TDLYLGNHSQLLETIREKMFDLDDDFIIIPGHGPHTTIGREKQTNPFLK"
     gene            complement(7974..8528)
                     /locus_tag="PXD66_001032"
     CDS             complement(7974..8528)
                     /locus_tag="PXD66_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HDL6319343.1"
                     /translation="MKQINVQRRKWLSLGGLVLGASLLPGQVMAALSTPAPAALRFRN
                     VNTGDTYTAKFGAGGLNKTDLSQLNYLMRDRHINQVKAIDPKLFVKLNQLQRRLGFHN
                     AEILVLSGYRSAQTNARLRRRSRGVASHSYHILGQAVDFQVSGVPLYKVKQAAESLNN
                     GGVGYYPRSNFVHVDTGPVRTWRG"
     gene            complement(8698..10203)
                     /locus_tag="PXD66_001033"
     CDS             complement(8698..10203)
                     /locus_tag="PXD66_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010129962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HDL6319344.1"
                     /translation="MVAFSGMALAETATVAQVASEPVVQDNILPQLSFAAQQARAAEL
                     VKQADYALEQEQIRQEQARKFAEAEARIKQEIGNNALLLSQATTKVYLDSEFATMWND
                     KAAEKAFLKEYALFAVSGVSSKSAKALQQILNQPEGLGRDILLTDGFLDYLYYNKNVF
                     KNANNWLYNLGSYVPKSPSSEQIFQWTNAVREGDSGQFVTDLVPRNHLYRDTAQRVLA
                     MAGGSSAPKAKKGKAVTEVAGGGNGASFYKLALNAQRLRIIPSFNNGIFVNIPSYQLY
                     YFRDGNLALQSKVIVGREDRRTPVMYSKLSNVVVNPPWNVPTSIMTKDIIPKLARDPG
                     YADRASFEILDSSGNKINPRSVNWSQYLNAKSSPYRIRQKAGDDSALGRYKFNMPSSD
                     AIYLHDTPNRGLFGKTGRALSSGCVRVEKSDELATILLKEAGWGSDKKNQVLGSKKTT
                     SAQIRSDNPVYLYYVTAWVEGGKVQTLPDIYNFDAKIPQHSVNWGKLKSII"
     gene            complement(10321..12333)
                     /gene="prc"
                     /locus_tag="PXD66_001034"
     CDS             complement(10321..12333)
                     /gene="prc"
                     /locus_tag="PXD66_001034"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HDL6319345.1"
                     /translation="MKLTRLNKIFALALGLMIGNAFAVQPDIKESAIVMPKPNEQHSL
                     STKRVTARLTQSHYHKFQLNDEFAGKIFNRYVDWLDPSHNTFLQADIDEMRSKYATTL
                     DDDLYAGKLDAAFAMYELMSKRRYERYSYALSLLDKEPDLKGNDEIEIDREKAAFPKS
                     VEEANTLWEQRVKNDIINLYLKDKKWPEIKKTLTKRYNFAIKRLTQVKADDILQTYLN
                     SFSREIDPHTSYLSPRAAKAFQESMNLSLEGIGATLTQEDDVTSIRALVPGAPAAKSK
                     KLVVGDKIVGVGQGERGEIEDVIGWRLDNVVDKIKGKKGTKVRLEIEPEKGGKTKIIT
                     LVRDKVRIEDSAAKLTVEKVDGKNVGVIKIPTFYIGLTSDVRKLLTQIEKKNVEGLII
                     DLRENGGGSLTEVIELTGLFIKDGPVVQVQDAFNRIKVHEDPESKSVYDGKVMVMINR
                     HSASASEIFAAAMQDYNRAIIVGQQTFGKGTVQQSRSLNFVYDLDQEPLGFIQYTIQK
                     FYRINGGSTQLKGVEADIKFPEIINAAKTGESFEDNALPWDKIPAANYKEAGNARNAV
                     AALTAKHQERIAKNPEFIVLNENIAIRKERDERKFLSLNLEERLKENKADESKRLKDI
                     NARFAREGKKALKDIDDLPKDYEAPDFFLKEAEQMMLDWSEFDKKS"
     gene            complement(12419..12943)
                     /gene="proQ"
                     /locus_tag="PXD66_001035"
     CDS             complement(12419..12943)
                     /gene="proQ"
                     /locus_tag="PXD66_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HDL6319346.1"
                     /translation="MEQKVEQTQNKTNPSTKEVIAYLAEKFPLCFTLEGEVKPLKVGL
                     FQELAEALKDDEKISKTLLRQVLRNYTNSWRYLAASQANVARVGLQGEEVGVVTEDEA
                     THAAEALAAAKAVVAERKAQERKAQRKEYFKQKAREENAKKRAEFKAKKAIDNLPKAS
                     EEKLAALADKFGRK"
BASE COUNT         3911 a         2739 c         2292 g         4006 t
ORIGIN      
        1 gatcagaatt aacctacatg tttatagata tcataatacc tgtttttcta atactggaga
       61 cgatacaatg aaaatcaaaa aattactact gccaactctg ttaagcattg ctactatgcc
      121 aattcaagca gccgacctac ctaatattac aattcttgca actggaggga caattgccgg
      181 tagccaaaaa aatacggcaa cctcagctta tcaagctggg cagctaaata ttgaaacact
      241 catagaagcc gtcccggata ttaaaaaatt agcaaatatc aaaggagaac aaatcgttaa
      301 aatcggatct caggatatgt ctgatgaagt atggctaaaa ctggcaaaag caattaatca
      361 acaatgccca aacacagatg gcttcgttat tacgcacggc acagacacta tggaggaaac
      421 cgtatacttc cttgatatga ctgtaaaatg cgaaaaaccc gtagtattag tgggggccat
      481 gcgaccagca actgaaaaaa gtgccgacgg cgtattaaat ttatataact cagttgttgt
      541 cgccaaagat tctaaatcta gtggcagagg ggtgttggtt gcaatgaatg atattgtatt
      601 gggcgctcgc gatgttacaa aaaccagtac tacatcagtg caaacattta actcaccaaa
      661 ctttggcgca ctaggctata tccataatag caaagttgat tatgagcgct ctcctgaaag
      721 taaacacacc gtaaacaccc ctttcaaagt agatgaatta acggaattgc caaaagtcgg
      781 cattatttat gcttattcaa atatgccaac cgaaccgctt caagcattat tagatgcgag
      841 ttatcaaggc atcgtggttg caggagttgg caatggcaat atgaatgcgg ctaatttggc
      901 tttacttgaa gaagcagcta aaaaaggaat taccgtggtt cgctcgtctc gagtgccaac
      961 cggatatact acccgagatg cagaaattga tgatagcaag tatggtttta ttgcttccgg
     1021 cacattaaat cctcaaaaag ctcgagtatt actgcagctc gccttaacac aaacaaatga
     1081 tataaaaact attcaacaat attttgaaga tttctaaaaa gtaaataaca agcggtctat
     1141 tttgcaaaac aaatcacaaa atagaccgct tgtctcatta ctaatcttta ttattttaca
     1201 aagctatcaa aagttagctc ataattgtcg ccatcacgac tataggcgat atagcgaggg
     1261 aatttttcac cttcaaattt tttcaccgta atgtcttttt tatcactggt tctaaacttg
     1321 taggtaattt cttggtaggt tttgccgcct acttttacct ctttttgggc tttgcgtagc
     1381 tgcacattcg gtgttgggta aagtttttta ccgttagtag tttgaaaact gctcggtaat
     1441 ttatcgtaat agctcaactg aaaggctgtg gtgaacaaat cataagttgg catagtcagc
     1501 gcttcttctt ttagtggctc tttcactttg ccatatttta ccgttttaga atcaatttct
     1561 gccatcgcat aagttttgcc gttacgggta tctcggtagc tcaacatatg aaattgttcg
     1621 tttttttctg ttcccttcgc agaaaagaca atattatata aaggcacatt aatttttgcc
     1681 tgaatattat attggccacc accgttatta aagttaatgt aggctggcat taaataattt
     1741 gagctgtatt tcaacttaaa attctccgca ctggcgtttg aaatcaagcc caaagtgacc
     1801 acgattacgc tcgctaactt ctgccaaatt ctcatctatt tctcctctaa agatatactt
     1861 tcgaacaacc ttgaaattgc attgttatct cgtaattgtt ccgctttctc cacttgtacc
     1921 cgtgttaaat gtggcgaaaa ataattaata aaatcataca tatagttacg aagaaaagtt
     1981 cctcgtttaa aggcgatttg cgtcatactc ggttgaaaca ggtgacttgc attgatagcg
     2041 actaagtctt tatccgtttc cgtatgtgcc attgatgcca taataccaac ccctaagcct
     2101 aagcgtacat aagttttgat tacatcagca tctgtcgccg taaatacgat attaggcaaa
     2161 ataccttcct tattgaaggc atgatctaaa tccgattgcc ctgtaaaacc aaaagtatag
     2221 gttatcaaat catatctacc taattgttcg acagtcaaat ttttactttg gttcacaaaa
     2281 gccgcaagag gatgctctgg tttgacaata actgaacgat tccaccaata acacggcaat
     2341 aaaaccgctt catcaaataa atattgagct tctgtggtaa tggctaaatc cacttctcca
     2401 gccaccaaag catcataaat ttgattcggg gagccttggt gcaaatgcaa acttacttca
     2461 ggatagcgag ccttaaactt ctcaattaca ctcggtagca tataacgggc ttgggtattg
     2521 gtggtcgcaa tgcgtaagat gcctttgttg ggtcgagttt gttcttcagc aaccgattta
     2581 atgctctggg cttttaccaa taattcacga gcaatcgcca caattttttc accagcaggc
     2641 gttagcccct taacgtgctt accgttacgc tcaaaaatat taattcccaa ctcactttcc
     2701 aacaaacgaa cttgtttgct aatccccggt tgagaagtgt aaagcacctc tgcggcctcg
     2761 gtaatattca agttctggtt tactatttca acgatataac gtaactgttg tagcttcata
     2821 atgatttccc ctctacaatc gcaataatct gttgcatcgt atgcacttcg taagttggct
     2881 taatatccgt atcattctct ttactaagca aatttaacca acaagtatca attccagcat
     2941 tattacctcc taaaatatca gaagcgaggg tatcgcctac cataagcact ttagtacgat
     3001 caaattcgtc cattaatgca aaagcgtaat caaacacttg gcgatcaggc tttgcggcac
     3061 caatttgttc tgatatcact acaatatcaa aaaaatgtga agtttgagta ttatctaaac
     3121 gtttttgctg caaatcggta aaaccattgg tgataatgcc catttttact ttaccatata
     3181 aagcatttaa catatccatt acaccttcta aaggctgact gacgaatgcc atctccgcca
     3241 ttaactcttg attaagttgt agcggatcta cgcctgtttg agcagataat tttacaaaac
     3301 gtctggtttg aatatcttga gcagtgatct cattattttg gtaagccacc caaagtggtt
     3361 tatttaccgc ttgaaagtct tgataatctt gttcactaaa atcaatttgg taacgagcca
     3421 gcatcgcttt taaaccaaga taagaattaa atgaaaacaa ggtttcatca gcatcaaata
     3481 atacccattg atatttcata atattccttt tttaacttag ataacaagcg gtcaaatttt
     3541 tctaattttt cgcataccgc tgataaagcc acgcttaagc acaacactct ttcatttttt
     3601 gctgaatcca ctcaacaaaa aattgtactt tctgaacttg ggctgattgc gaaggataga
     3661 caacatagaa cgatttttca tcaaacaatg tggttgggaa aggctctatt aaagtacctt
     3721 tttcaatttc gtgctgtgcc aacaactggt tggcaatcgc aatgccttgt tggtgcattg
     3781 cagcttgaag caccataaaa gtatggctaa aaattgagcc agtttccgca tctactatat
     3841 cttctatatg caaatgttca accattcttt tccacttatt atgggtggag gaatgtaata
     3901 aatgcttacc tgctaaatcc gccggtaaat gaatggtatt ttgcgataaa tactcaggcg
     3961 aagctaaaat aacaatacgg gattccgtca aacggataga ttccagacct tgccaatccc
     4021 ccttaccata ataaatagca acatctgttt ctttacctaa taatccctca tcttgatcaa
     4081 cacccttaat acgtacttcc acttctggat atttttcgtg gaaatccgcc aaacgtggga
     4141 ctaaccaatg cataccaaat gtttgtggta cgctaatagt cagaatttga cgactacttt
     4201 gctgcctaat tttatctgtt gcgagagcaa tttgttctaa taatggctga acttcataga
     4261 aatattgtgc gcctaactca gttaattcca gcaaacggtt acggcgatgg aacagggaag
     4321 cacctaaaaa atcttctaat agtttaattt ggtggctcac tgcagcttgt gtgacgaaga
     4381 gatcttctgc cgctttagta aaatttaaat ggcgtgcagc agattcaaat gctttcaatg
     4441 cattgagtgg tggaagtttt ttgttcataa tttttcaatc taatagatta ctaaacaagc
     4501 aactctatta ttttttctga tagtttaaat aaaaaaaatt cgtttgatgc ttgcaaatag
     4561 aaataataaa atatccgcat acttagatgg atagtttaga atttatttct attcctaaat
     4621 tttaagtatg atgttgtgtt tgcatattgg tctaggaaac tagactagag taacgaaagt
     4681 tacttgtttc acttcctgta tattttgaac cgtattggtt tataaccgcc cactttgggc
     4741 ggtttttttc tatgtaaaaa aacagcacaa aacctagatc gcattctata ttattcgcta
     4801 cctttattct agcctttctg cttttttatt ctttcacttt aatctattat aaaaagaggt
     4861 tttatagtat gctaaagcct atttttcatt tgcaataatt accgagagtt cttttgagta
     4921 aaccgaaatt aacccataac caacagcgta gaatccaatc taaccatcat aagcgtatca
     4981 acaaaaaaga gattgaatgg caagatgaaa tgttaggaga aatgcaacag ggcatcgtcg
     5041 ttactcgcca cgttaaacac gctgatgttg aaaccgcaga ggggcaaatt ttccgctgta
     5101 atatccgccg tacattgaaa aatgtagtgg tgggcgatca ggtttcttgg cgtaaaggta
     5161 gtgagcaact acaagggatt agtggggtca ttgaagccgt cttcccacgt aaaaatgaac
     5221 ttacccgccc cgattattat gacggtatta aggtgatggc atcgaacatc gatcaaatca
     5281 ttattgtatc agctgtgcta cctacacttt cgctgaatat tatcgaccgt tatttagtga
     5341 tttgcgaaac ggcgaatatt cctgccttaa tcgtgctaaa caaaattgat ttattaaacg
     5401 aaagcgaacg gcaagcagtt aaagagcagc tcaaaatcta tgaagagatt ggctatgaaa
     5461 ccctatgttt atcggctgat acgggtgaaa atatgcaggc gttacaccaa tatcttgcta
     5521 aaggtacgtc tatttttgtc ggacaatccg gcgtggggaa atccagccta attaaccaac
     5581 tgttacctga agtcaatgct ctcactggct cagtgagcga taactcaggg ttaggtcagc
     5641 acaccaccac tgcctctcgc ttataccatt taccgcaagg cggacagctg attgactcgc
     5701 cgggcattcg agaatttggc ttatggcatt tagaacctga gcaaatcacc ttaggctatc
     5761 gagaatttca atcggtttta ggcacgtgca aattccgtga ttgcaaacac aaaaccgacc
     5821 cgggttgtgc cttaagacaa gaggtcgaaa atggcgaaat ttctgcaatt cggtttgaaa
     5881 attaccatcg cttaattgaa agcctgtctg aaaccaaaag ccaacgccat ttcagaaaag
     5941 aagaagtata atgaatgaat ttatttaccc taacgcccga ttaatcgccg gtgttgacga
     6001 agtcggacga ggacctttag tcggagcggt tgtgaccgca gccgtgattt tagatccgaa
     6061 taacccgatt cagggcttaa ccgattccaa aaaattatcc gagaaaaaac gccaagcttt
     6121 ggcagaagag attaaagcca aggctctgag ctggtcgcta ggacgtgccg agccgacaga
     6181 aattgatgag ctgaatattt taaatgcgac aatgttggca atgcaacgtg cggttgccgg
     6241 cttgcatatt cagccggatt ttgtgctaat tgacggcaat cgtccgccaa aactgccaat
     6301 gccggcacaa gcggtggtga aaggagacag cttagtggct gaaatcagtg ctgcttcgat
     6361 tattgccaaa gtcgcccgag atcaagaaat ggacgaatta gaccaacaat ttccgcaata
     6421 cggcttcgca cagcacaaag gctacccgac taaactccat tttgaaaaat tagcagaatt
     6481 tggggcaaca ccttatcatc gtaagagttt tgcccctgta agaaaagtat taggtttaat
     6541 cgattagaag gaaaacacaa tgactacaga gtaccaaaaa acgattttca ccaaaatcat
     6601 caataaagaa attccggcaa atattgtcta tcaagatgaa cttgtgaccg catttcgtga
     6661 tattgctcca caagcaccca cgcatatttt gattattccg aataaattta ttccaaccgt
     6721 caatcacgtg agtaatgaag atgaattagc cctagggcgt ttatttacgg tggcggcaaa
     6781 attagcccaa gaagaaggca ttgccgaaga aggctatcgc ttagtgatga attgtaataa
     6841 aaacggcggg caggaagtgt tccatattca tatgcacttg ttaggcggac aaaaactagg
     6901 acctttagtg gcaaaataac tatgcgtatt tcctactttg catttgcaat tttttcgatt
     6961 tttttgaccg cttgtggtag caaacctgtc agctatttac caaccggctc agagcctatt
     7021 gtcaatattg agtctgacat tgctgaaaag atagagctgg aaacgaatag caagcaattt
     7081 agcaccacaa atttaactga atttccgctt aatcttactt ataaattatt ttggtataat
     7141 aaagatgggg taacacaatc ccataacggt atatcagata gtatcggctg gcaacatcta
     7201 tggttgcaac ctaagcaaaa acagaccatt gcactcatta aaccgacgga agaaagccat
     7261 aattaccgcc tctatttacg aggcgtaaaa tagcaaaaaa gcagagccaa ctgctctgct
     7321 tctttattta tttcaaaaac gggttggtct gtttttcccg cccgatagtt gtgtgtggac
     7381 catgtcccgg tatgatgatg aaatcatcat ctaaatcaaa cattttttcc ctgatcgttt
     7441 ccaataactg gctatggttg cctaaataga gatctgttcg acctatgcta ttttggaata
     7501 aaacatcgcc actaaaagca atacgattgt caaaatcaaa aaaaccgata tgacccggag
     7561 catgacccgg caaatgtcga acggtatagc tcagctcgcc gacttcgacc cgatcacctt
     7621 cctccaacca atgatccggc aaaaagccat ttactggtgg taatccaaat tgttggcaca
     7681 tttccggcaa acgttcaaat aaaggtttat ctgcaagatg cgagccgaaa acctctactt
     7741 gaaaatgctc tctcaacttt tccaccgcca taatatgatc taaatgaccg tgcgtaatca
     7801 ataatttctg aaccttaagc tgctgagatt caacaaattg aataatgcct tcggcattat
     7861 cgccgccatc aataatagcc gcattcttat tttcgtccca aatcaccgtg caattttgtt
     7921 gaaatggact aacaggaata atttgcaaat taaacataaa ataagaccgc ttgttagcct
     7981 ctccaagtgc gaactgggcc agtatctacg tgaacaaagt tactacgagg ataataacct
     8041 acgccaccat tattcaagct ctcagccgct tgttttactt tatataacgg cacacctgat
     8101 acttggaaat ctactgcttg cccaagaata tggtaactat ggctggcaac accacggctt
     8161 ctacgacgaa gtcttgcatt tgtttgtgcg gagcgatagc cacttaatac taggatttcc
     8221 gcattatgga aacctaaacg gcgttgtaat tgattcaatt tgacaaataa ttttggatca
     8281 attgctttaa cttgattgat atgacgatcc cgcattaaat agtttaactg gcttaaatcg
     8341 gttttattta atccacctgc accaaattta gcagtgtaag tatcgcctgt attcacatta
     8401 cgaaaacgta acgcagctgg ggcaggagta gaaagcgctg ccattacttg acccggcaga
     8461 aggctagccc ctaaaacaag tccgccgagt gatagccact tacggcgttg aacattaatt
     8521 tgtttcattt tttcctcata ggttagctaa tgatttatag aaaatcactc acaaatgagc
     8581 gaatttctat acaccatact taaagtaaaa atttacaatc gtaaatttat gtaagctata
     8641 ttttcaaaaa gttccaagaa gatgatatct tcttggaatt tatcagaaat agcacgatta
     8701 aataatgctc tttaattttc cccaattcac agaatgttgt gggattttgg catcaaagtt
     8761 ataaatgtcc ggaagggttt gcacttttcc accttcaacc caagccgtca cataatagag
     8821 ataaaccggg ttatctgaac gaatctgagc cgaggtcgtc ttcttactgc ctaagacttg
     8881 attcttttta tcagatcccc aacccgcttc ttttaataaa atggttgcca attcatcaga
     8941 tttttctaca cggacacaac cagaacttaa cgcacgcccc gttttaccaa ataatcctct
     9001 atttggcgta tcatgcaaat aaattgcatc cgaacttggc atattaaatt tataacgtcc
     9061 taaagcgcta tcatcacctg ctttctggcg aatacggtat ggtgaacttt tagcatttaa
     9121 atattgtgac caatttacgc tacgcggatt aattttattg ccactactat caagaatttc
     9181 gaaactagca cgatctgcat aacctgggtc acgcgctagt ttaggaataa tatctttagt
     9241 cataatacta gtcggtacat tccaaggtgg attcactacc acattgctca gcttactata
     9301 cattaccggt gtacggcgat cttctcgccc aacaatcact ttagattgaa gagctaaatt
     9361 accatcacgg aaataatata attgatagct tgggatattc acaaaaattc cattgttaaa
     9421 acttggaata atacgtaaac gttgagcatt gagcgctaat ttatagaaag aagccccatt
     9481 accgccacca gctacctccg taacagcttt accttttttg gctttaggtg ctgaagagcc
     9541 tcctgccatt gccaatactc tctgtgccgt atcacgatat aaatggtttc ttggaacaag
     9601 gtctgttaca aactgcccag aatcgccttc cctcacggca ttagtccatt ggaagatttg
     9661 ctctgaagag ggcgattttg gtacataact gcctaagtta tataaccaat tatttgcatt
     9721 tttaaacaca tttttgttgt aatacaaata atctaagaag ccatcagtta ataaaatatc
     9781 tctacctaaa ccttccggtt gatttaagat ttgctgcaat gccttagctg atttagagga
     9841 taccccactt actgcaaata atgcatattc tttcaaaaat gctttttctg cggctttatc
     9901 attccacatt gtcgcaaatt cactgtctaa ataaacctta gttgttgctt ggctcagcaa
     9961 taaggcatta ttacctattt cttgcttaat tcttgcttcc gcttctgcaa attttcttgc
    10021 ttgctcttga cgaatttgtt cctgttctaa cgcataatcg gcctgtttta ccaattctgc
    10081 agcacgagcc tgctgagctg caaaacttaa ttgtggtaaa atattatctt gtacaacagg
    10141 ctcagaagcc acctgagcaa ctgtggctgt ttcagccaaa gccatgccag aaaacgccac
    10201 cataataggt aataatttaa aggttttcac ccactcttcc tcttaatatt tgcaaattta
    10261 taaaaaaata aaccgcttag cggagctaag cggtacgtta tgtgcgcatt ataaacacat
    10321 ttatgatttt ttatcaaatt ctgaccaatc aagcatcatc tgttcagctt cttttaagaa
    10381 aaagtccggt gcttcatagt cttttggtaa atcatcaata tcttttaatg cttttttgcc
    10441 ttcacgggca aaacgagcat taatatcttt taagcgcttg ctttcatctg ctttattttc
    10501 tttcaggcgt tcttctaaat ttaacgataa aaacttacgc tcatcacgtt cttttcgaat
    10561 cgcaatattc tcgtttaata ctataaactc tgggttttta gcaatacgct cttgatgttt
    10621 cgctgttaat gcagcaaccg cgttacgagc attcccagcc tctttataat tagctgctgg
    10681 aattttatcc caaggaagcg cattatcttc aaaactttca cctgtttttg cagcattaat
    10741 gatttccggg aatttaatat ccgcctcaac cccttttagc tgggtacttc caccattgat
    10801 acggtagaat ttttgaatcg tatattggat aaaaccaaga ggctcttgat ccaaatcata
    10861 tacaaaattc aatgaacggc tctgttgaac agttccctta ccaaaggttt gttgccctac
    10921 aataattgca cggttgtaat cttgcattgc cgcagcgaag atttcagagg ctgaagcact
    10981 atggcgatta atcatcacca tcacttttcc atcataaact gattttgatt ctggatcttc
    11041 gtgtactttg atacgattga atgcgtcttg tacttgcact acaggcccat ctttaataaa
    11101 taaaccagtt aattcaatca cttcagttaa cgaaccacca ccgttttcac gtaaatcaat
    11161 gatcaatcct tcaacatttt ttttctctat ttgagtaagt aatttacgta catctgaagt
    11221 caaaccgata taaaaagtag ggatcttaat tacccctaca ttttttccat ctactttttc
    11281 taccgttaat ttagcagcac tatcctcaat acgaacttta tcacgcacaa gggtaataat
    11341 tttagttttt ccaccttttt caggctcaat ttctaaacga actttagtgc cttttttacc
    11401 tttgatttta tcgactacat tatccaaacg ccaaccgata acatcttcaa tctcacctct
    11461 ctcaccttga cctacaccga caattttatc gccgactacc agcttcttac ttttagcggc
    11521 aggggctcct ggcactaatg ctctaatgct ggttacatca tcttcttggg ttagcgttgc
    11581 accaattcct tctagcgaca gattcatgct ctcttggaac gcttttgccg cacgaggaga
    11641 caaatagcta gtgtggggat cgatctcacg agagaatgaa tttaagtaag tttgtaaaat
    11701 atcatcagcc ttaacttgag ttaaacgctt aatcgcaaag ttataacgtt tagtgagtgt
    11761 tttcttaatt tcaggccatt ttttatcttt aagatataaa ttgataatat cgtttttaac
    11821 tcgctgctcc caaagtgtat ttgcctcttc tacacttttt ggaaatgcgg ccttctcacg
    11881 gtcaatctca atttcatcat tcccttttaa gtcaggctct ttatctagta aagataaggc
    11941 atagctataa cgctcataac gacgtttaga cattaactca tacattgcaa atgctgcatc
    12001 taatttacca gcatataaat catcatctaa tgttgtagca tatttactac gcatttcatc
    12061 aatatcggct tgaagaaaag tattatgaga aggatcaagc caatcgacat agcggttaaa
    12121 aatcttccct gcaaactcat cattcagttg aaatttatga taatgcgatt gagttaatcg
    12181 ggctgttaca cgcttggttg ataagctatg ctgctcatta ggttttggca tcacaatcgc
    12241 actttcttta atgtccggct gaacggcaaa agcgtttcct atcattaaac ctagggctag
    12301 ggcaaaaatt ttatttaatc ttgttaattt catcttcttt tccaaataat caagcggtca
    12361 tttttttgca aaattttact aaaatccgac cgcttgtaag cacaaagcga cttgaaaact
    12421 atttacgacc gaatttatct gctaatgcag ctaatttttc ttctgaagct ttaggtaaat
    12481 tatctattgc ttttttcgcc ttaaattctg cacgcttttt cgcattttct tcacgtgctt
    12541 tctgtttgaa gtactcttta cgttgtgctt tacgctcttg tgctttacgt tctgcaacca
    12601 ccgctttcgc agcagccaaa gcttctgcgg cgtgagtagc ttcgtcttct gttaccacgc
    12661 caacttcttc accctgtaag ccgacacgag caacattagc ttggctggca gccaagtaac
    12721 gccaagaatt agtgtaattc cgcaatactt ggcgcaataa cgttttactg attttctcat
    12781 catcttttaa tgcttcagct aattcctgaa acaacccaac tttcaatggt tttacttcac
    12841 cttcaagcgt gaaacaaagc ggaaatttct ccgctaaata agcaatcact tccttggtac
    12901 ttggattggt tttattctga gtttgttcta ctttttgctc aatttgct
//
LOCUS       DANLXF010000058        12612 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.058, whole genome shotgun sequence.
ACCESSION   DANLXF010000058 DANLXF010000000
VERSION     DANLXF010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12612
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.058"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            83..328
                     /locus_tag="PXD66_001036"
     CDS             83..328
                     /locus_tag="PXD66_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319347.1"
                     /translation="MKLMKDMGTQSYIWSGFSTWLAWLGEQQNLMLLSLAIGIVTAIA
                     GLIQRFDEWRRRKREDERAEELHQAKMARLRKGLSDE"
     gene            321..890
                     /locus_tag="PXD66_001037"
     CDS             321..890
                     /locus_tag="PXD66_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HDL6319348.1"
                     /translation="MSKSLKYGSGIVCGIAAIITLVQYQHPEIRTNQAGLEIIGNAEG
                     CRRDPYKCPADVITVGIGSTEFGGEKIDPNRIYSDKEIAERWAKDLKIAESCVNRHFN
                     GKDMNDNQFSGMTSAVFNMGCYNMRFYRNKQGQYVQTTIHKLAVNKQFEEMCHRLPDF
                     IRASGKVLNGLVIRREKEKALCLTGLVAR"
     gene            863..1213
                     /locus_tag="PXD66_001038"
     CDS             863..1213
                     /locus_tag="PXD66_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HDL6319349.1"
                     /translation="MLNWISGTIAIVILGLCVWLWGQSQMIDSLRAENRAQAQTIEQQ
                     QKANQRLTDSLEQERQAVEKIQKIANELRNKVEMAKNEITSILAQDSCAKADLPNGVA
                     DSIKRLHQQNNHKH"
     gene            1218..1391
                     /locus_tag="PXD66_001039"
     CDS             1218..1391
                     /locus_tag="PXD66_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319350.1"
                     /translation="MYPPAAYLVPCGRTEFSGRTYGDTVEYLVKVMGERDLCANQIDR
                     IREWQAQTKQGFK"
     gene            1783..2307
                     /locus_tag="PXD66_001040"
     CDS             1783..2307
                     /locus_tag="PXD66_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDL6319351.1"
                     /translation="MTKKGEVKATSKGVGKLTDKQKRFVEEYLIDLNATQAAIRAGYS
                     EKTAYSIGEENLRKPEIRSAIQEAQNKRSERTQITQDDVLNGLLEVIAMSTGKKIVTE
                     TDVAKNENGELVGFDIAKTKFEPAAANKALELLGKHLGMFKDKVDLINSDGSLNRPMV
                     IELIAPSLDDESTD"
     gene            2291..3511
                     /locus_tag="PXD66_001041"
     CDS             2291..3511
                     /locus_tag="PXD66_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HDL6319352.1"
                     /translation="MKAQIELPPKLLPVFAKDYRYKGAYGGRGSGKTRAFAKMSAVSA
                     YKRAMQGESGVILCGREFMNSLEESSLEEVKQAIKSEPFLANFFDVGEKYVRTKDGRI
                     SYVFSGLRHNLDSIKSKARILIAWIDEAETVSETAWRKLIPTVRENNSEIWVTWNPEN
                     RGSATDTRFRQNPPENSCIIEMSYQDNPWFPDVLEQERLNDKQRLDDATYRWIWEGAY
                     LEASEAQIFRGKYQEMEFVPNPDFDGPYYGLDFGFAQDPTAAVKCWVFNGDLYIEFEA
                     GKVGLELDDTADFIAKGVPQIADHVVRADSARPESISYLKRHGLPRIIGVDKWKGSVE
                     DGIEHIKSYGKVFIHPRCQQTLNEFRLYSYKTDRLSGDVLPVVIDAHNHFIDALRYAL
                     TPLMQVKSAKGVLL"
     gene            3508..4884
                     /locus_tag="PXD66_001042"
     CDS             3508..4884
                     /locus_tag="PXD66_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HDL6319353.1"
                     /translation="MSLEQDRLAFLAEALGLGNIKRRTLWKEFGYPNTLTFNHFLKAY
                     KRNSIAFAAINRLLDGCWVDCPVIVEGEQKNESKQTTEWESKVERFMKRYWSAIKEAD
                     RRNLVGNYSALLLQVRDGQQWDQPILQGALSSIGEFGLVKLIPVWQSQLSVTEFQEDV
                     TADNYGEPLMYQFSESAFGKKSVSRNIKVHASRVILLNEGGDFNSPHSGVPLLEAGYN
                     KLVDLEKTSGGSAEGFLKNASRQLGIKLTKDVDLRQLEDSAKALGFSSFSDALNDKIK
                     KINSGTDSALITHEGDASVLSVAPADPKPTWEISANEFAASVQIPFTILFGQQTGRLA
                     SDEDKTDWANRCNGRRNGFLTDVITQLLNRLWFIGVLPMPKNSDVTVSWSDLLAPSEK
                     EKIANAQALASVATTSQSAFGFAAVTANEIRESLGFEPLPDNLIPPTIDDENEDQDEN
                     QATADSDK"
     gene            4838..5776
                     /locus_tag="PXD66_001043"
     CDS             4838..5776
                     /locus_tag="PXD66_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HDL6319354.1"
                     /translation="MKIKTKIKPLRIPTNKADPLNMGVSVAKLFRHIDVIYSQIKRSI
                     KQQILQKMSLRLQANNFEYFPSYLTATELADLLETLQKIIDKEILSNGSQGENLWFDS
                     YIDEAALKGTQSAVTDLGLQSEAYRSQRNLSSIVFSPAYFNRLAIAHMASYSEWQGLS
                     DELRKSLAGVITEAVLNGKNVKETAREIRQKLDVSSKRAKRIAQSSQLAAYRRAEWQE
                     AEEAKEELGLNTKLLHFSALKATTRLAHAQRHGKYFDVAEVREWYSRDGNQFNCYCKQ
                     SVVVVNADGKTDIEPLLVGLAKERKQWVGALKNVKK"
     gene            5763..7133
                     /locus_tag="PXD66_001044"
     CDS             5763..7133
                     /locus_tag="PXD66_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016533290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HDL6319355.1"
                     /translation="MSKNNVNIVTVINSKNISTESIDGDQHLIIRGVVPIVDDVVMND
                     GLYPADEINKSYNSLEGNFMPLGHPKIEGKYVSAQDVRAVNKHHVGAWAKNVRKESGK
                     VLVDMYINRRFAEGSEQGKTLLERLDGMVTNTNAEPIHVSTGLILNKVKQSGKSKGKK
                     YSWIATNMSFDHIAILLDVPGAATPDDGVGIFVNQDGSEMGVENVNLTQAADYRTEKL
                     FDKVRFYLMANSDLSFEEIHRALREAIRPEGKDKWSFWVEAVYPKYFIYSDDNSGKKY
                     KRSYFIDENDNVQLTSEPVEVVKKIGYEEITTNEEHNPMKTKILAVLNAANVKTEGLN
                     DDQLLEAYNKLQADKGKEPEKPKNEDDSEIDEKIKKAVNAAIEPLQQALQANTDKEQA
                     AMRAAVKAKFNMSDAAVNALQGEVLTELYAQTQQSVGLNNNSANGNGDNQWDGYSLNQ
                     EEKGNE"
     gene            7126..7560
                     /locus_tag="PXD66_001045"
     CDS             7126..7560
                     /locus_tag="PXD66_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319356.1"
                     /translation="MSNVIYRGSVKREPQTVNIVINDTSAPGAVVKLHNGKLEAAADL
                     KGRRFLLGNNRFKGQTIEQAYAKGDTATAFRLEPEQEYYAQIADGTYNFGDELTVKQA
                     GGKLTKAETGDVVLFFFDEESQRVISGGKGYADVVVANAYTK"
     gene            7575..8564
                     /locus_tag="PXD66_001046"
     CDS             7575..8564
                     /locus_tag="PXD66_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="encapsulin"
                     /protein_id="HDL6319357.1"
                     /translation="MLKFTKEQERFVINERINYDKKHALMAANSAGLLGNAMTLPKDV
                     WAEWDRDAVQIQREELVVFNDLAGISKSMPIGKLIHHFQTVSDSGSVNISLDGRSSAK
                     TDNVVLNYHGTPLPIIDSTFSFGWRDMSAAQSEGYQIDGAARANSLRKVAEKMEDLAL
                     NGDSSIVVGNAKLYGLRTAPNRMTNTHNLDLATATPKEIYDVFRDLISKFHAKNYYSP
                     VTLYVNYGDYFAMSTRDYSEQKSEGSILNKVMTIPQIAKIVPASRVPQNEILGLCKRS
                     DVYQVLNGMPLVTRPIARHNETDDYSFQIMAAVAVEFKFDDKGNAGYIQYTKS"
     gene            8576..8836
                     /locus_tag="PXD66_001047"
     CDS             8576..8836
                     /locus_tag="PXD66_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319358.1"
                     /translation="MKWLLTHDSHELKKGDVYEGETLPLWLVGKAIEIEEKSFEVATP
                     SDHADGETGETGETGETGETGETGETGETGETGQKGKKDKGK"
     gene            8839..9216
                     /locus_tag="PXD66_001048"
     CDS             8839..9216
                     /locus_tag="PXD66_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319359.1"
                     /translation="MAAEIELSEARTTLDELGFTSPNSLLELYVQQTNTLDQALNKSG
                     YSEETQKLIKLYLVAILAISSGARRIKSEGAPSGASRTFEYDPNALDNLKSAIRSIDP
                     KGCAESLLPKDKVVGFFDVVGGC"
     gene            9216..9560
                     /locus_tag="PXD66_001049"
     CDS             9216..9560
                     /locus_tag="PXD66_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319360.1"
                     /translation="MSNLSNWSYTAKATLWQAKGKNDDGVLVFSTPQIISCDYGADRK
                     RARFEIGREQAVKNVIWTEFAHAKLGDYVLIGESEQADPILAGAEEIIHIQRFADTFD
                     RNRDDFALITGG"
     gene            9562..9930
                     /locus_tag="PXD66_001050"
     CDS             9562..9930
                     /locus_tag="PXD66_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDL6319361.1"
                     /translation="MGIKVKGIRQVKQRLNQVVGEVQSQKATRAMFRILNAVAPLAAH
                     YTPVDTSTLINSQFTEMEVNGTRLIGRVGYSANYAAYVHDPNIKQNFRKPSAKKEFLT
                     SALNESKATIKSIVKEELSL"
     gene            9927..10307
                     /locus_tag="PXD66_001051"
     CDS             9927..10307
                     /locus_tag="PXD66_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319362.1"
                     /translation="MISYVKAFKQWLEAHSLADGYVVQLYQWEDTQKAKPVIVIQPNS
                     GSPQVSDLSSEHYLLISLVAGKNSGYTIEERARAIMSKILVEPFASFGYIESMGGLPA
                     PIFTEDNRMIFRLPLRIISTNQEE"
     gene            10311..10793
                     /locus_tag="PXD66_001052"
     CDS             10311..10793
                     /locus_tag="PXD66_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6319363.1"
                     /translation="MGSPSPQNNAENLVVGRAVVLEYGTTDAKPQESEWKVAGAMTTK
                     SWDFSPNSVTSEADDAGGFPETLITNSDFSISGDGEWRKRPKSKELGIKDLVVLYANA
                     VKNRTQPYIWVRLKYAGLTILGKMVITALSSEAPTNDLVKFSIEFKVGDSSTIEIAAA
                     "
     gene            complement(10833..11249)
                     /locus_tag="PXD66_001053"
     CDS             complement(10833..11249)
                     /locus_tag="PXD66_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDL6319364.1"
                     /translation="MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLL
                     SCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKVLLHNTMQEQAVSKAEIARLTNI
                     RPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG"
     gene            complement(11305..11481)
                     /locus_tag="PXD66_001054"
     CDS             complement(11305..11481)
                     /locus_tag="PXD66_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010848235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDL6319365.1"
                     /translation="MKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELK
                     TGLVEGVKKQLGLK"
     gene            11577..12248
                     /locus_tag="PXD66_001055"
     CDS             11577..12248
                     /locus_tag="PXD66_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319366.1"
                     /translation="MKMVSYPITEIGEMLIATDGRDFLFRPSFKNIYKIGSPQEIVKI
                     YALLHGCEIREILPHILTKSESHQSYFWSVLNSPVFGRKILQNAMIVMSCCCDDDISL
                     LIGEWLPSKSGVRYKLGKMPPETIINFAKNLMGHGVIGCCELPKKEQSAKNEFSESFD
                     AVEYISLARTHFNMSREDAENLTMTELQQLLKSQMPAENNSYGQFTEEEYDRIMAEYE
                     KRRAA"
     gene            complement(12263..12496)
                     /locus_tag="PXD66_001056"
     CDS             complement(12263..12496)
                     /locus_tag="PXD66_001056"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319367.1"
                     /translation="MKKDCTKQDFANWLNENQEVCWIKEVSIERILSCVDDYVAICNA
                     RSKDEFELLNDIRELFYSAHYKDFTPVLSGRFF"
BASE COUNT         3801 a         2527 c         3042 g         3242 t
ORIGIN      
        1 gggaacagta ttctagcaga atttttaacc aaagctcagt ctcacgactg ggctttttta
       61 ttgcctcgaa acggggtgga gtatgaagtt aatgaaagat atgggaacgc agagctatat
      121 ttggtcggga tttagcactt ggctggcgtg gctcggcgaa cagcagaatt taatgttact
      181 tagtcttgct atcggtattg ttactgccat tgcagggctt attcagcgat ttgatgaatg
      241 gcgtaggcgt aagcgtgaag atgagcgagc ggaagagctt catcaagcga aaatggcaag
      301 attgagaaag gggttaagtg atgagtaaaa gccttaaata tggcagtggt atcgtttgtg
      361 gtatcgctgc cattattaca ttagtccaat atcaacatcc cgaaatccgc actaaccaag
      421 ctggattaga gattatcggt aatgctgagg gttgcagacg tgatccgtat aaatgccctg
      481 ccgatgtaat cacggttggc attggctcaa cggagtttgg cggtgaaaag attgacccaa
      541 atcgtattta ttctgacaaa gaaattgctg agcgttgggc aaaagatttg aagattgcgg
      601 agagttgtgt caatcgtcat tttaatggta aagacatgaa cgataatcaa ttttcgggaa
      661 tgacgtccgc tgtatttaat atgggttgtt ataacatgag attttatcgg aataaacaag
      721 gtcaatatgt gcagactacc atccataaac tcgcagtgaa taaacaattt gaggagatgt
      781 gtcatcgact acctgacttt attcgtgcca gtggtaaggt attgaacgga ttggttatcc
      841 gccgtgaaaa ggagaaagca ttatgcttaa ctggattagt ggcacgatag ccattgtgat
      901 tttgggcttg tgtgtatggt tatggggtca gtcacagatg atagatagct taagagctga
      961 gaaccgcgca caagcccaaa ccattgagca gcagcaaaaa gccaatcaac ggctaaccga
     1021 tagcttagag caagagcgac aagcggtcga aaaaatccaa aaaattgcaa atgagctgcg
     1081 gaataaagtg gagatggcca agaatgaaat tacatcaata ctggcacaag acagttgtgc
     1141 taaagctgat ttgcctaatg gcgttgctga tagcattaag cggctgcacc agcagaacaa
     1201 tcacaagcac tgagtatttg tatccgccgg cagcttacct tgtgccgtgt gggcgaacag
     1261 aatttagcgg tagaacctat ggcgatacgg ttgagtatct cgtcaaagtg atgggagagc
     1321 gtgatttgtg tgctaatcaa attgaccgta tcagagaatg gcaagcacaa accaaacaag
     1381 ggtttaaata gctaattatc taattaaccg ctattgaaaa tgggagcgta acagctccct
     1441 ttttgttttg atggaaagta aatattacat aatttagcta tggtttcggg gttggttgga
     1501 taaaacaccg aggaaatacc taagtgatgt gatttatata aaacaaaacc ccgaacattt
     1561 gcggtgttcg aggttttttt attacccatt gaaacggaat gagtaacaag ttatggaaat
     1621 tattacattt ttaaccgtaa ccatcaagga gattcttatg gaatatggtt tatggcaaat
     1681 aagcctagca gtaacagtcc ctattttggc gttcgtgtcg ccaaagctga ttaatgctat
     1741 tgctaatcta ttaaatgcaa tgaagtaaag gaatttaacc caatgactaa aaaaggcgag
     1801 gttaaagcca cgtctaaagg cgtgggtaaa ttaactgata aacagaaacg atttgtagaa
     1861 gagtatctca ttgacttgaa tgcgactcaa gctgctatta gagctgggta cagcgaaaaa
     1921 acagcatact caatcggaga agagaacctg agaaaacctg aaatcagaag tgcaattcag
     1981 gaggctcaaa ataaacgctc ggagagaacc caaattacac aagatgatgt gctaaatggg
     2041 ttgcttgaag tgattgcaat gagtactggt aaaaaaattg tgacagaaac tgatgttgcg
     2101 aaaaatgaaa atggcgagtt agtcggtttt gatattgcaa aaacaaagtt tgaacctgct
     2161 gctgcgaata aagctcttga gttactcggt aaacatttag gaatgttcaa agataaggtt
     2221 gatttaatta attctgatgg ttcactgaat cgaccgatgg tgattgaatt aattgctccg
     2281 agtttagacg atgaaagcac agattgaact accacctaaa ctgttgcctg tttttgccaa
     2341 agattatcgt tacaaaggtg cttatggtgg gcgaggttca ggcaagactc gggcatttgc
     2401 caaaatgtcg gcagtgtctg cttataaaag agcaatgcaa ggggaaagtg gcgttattct
     2461 ttgcggtcgt gagtttatga actcattgga agaaagctct cttgaagaag ttaaacaggc
     2521 gatcaagtct gagccttttt tagcaaattt ttttgatgtc ggcgagaagt atgttcgcac
     2581 taaagatggg cggatttctt atgtgttttc cggacttcgg cataacttag atagcatcaa
     2641 atctaaagcc cgtattttga ttgcgtggat agatgaagca gaaacagtaa gcgaaactgc
     2701 ttggcggaaa ttaattccaa ccgtgcgtga gaataattcc gaaatatggg taacttggaa
     2761 cccggaaaat agagggagcg caacagatac tcgcttcagg caaaatccac ctgaaaacag
     2821 ctgcataatt gaaatgagtt accaagataa tccttggttt cctgatgtgc tcgagcaaga
     2881 gcggctaaat gacaaacagc ggcttgatga tgcgacttat cgctggattt gggaaggtgc
     2941 ttatttggaa gcgagcgaag cgcaaatctt tcgcggtaaa tatcaagaaa tggaatttgt
     3001 gccgaaccct gattttgatg gcccatatta cggcttagat tttggtttcg cgcaagatcc
     3061 gacggcggcg gtcaaatgct gggtatttaa tggcgatctg tacatcgagt ttgaagcagg
     3121 caaagtgggc ttagaacttg acgacaccgc agactttatt gccaaaggcg tgccacaaat
     3181 tgccgatcac gttgtgcgtg cagattcggc gcgtcctgag tcaattagtt acctcaaacg
     3241 tcacggctta ccgcgcatta tcggcgtgga taagtggaaa ggctcggttg aagatggcat
     3301 tgagcatatt aagtcctacg gcaaagtctt tattcaccca cgctgtcagc aaacgctcaa
     3361 cgaatttcgc ctttattcgt acaaaaccga ccgcttgtcg ggcgatgtat tgcctgtcgt
     3421 gattgatgcc cataaccact ttattgatgc gctacgctac gccttaacgc cgttgatgca
     3481 agttaaaagt gccaaaggag ttttactatg agcctagagc aagacagact ggctttttta
     3541 gccgaagccc tagggcttgg caatatcaaa cgaagaacgt tatggaagga gtttggctac
     3601 cctaatacct taacattcaa ccatttctta aaagcctata agcgcaatag tatcgctttt
     3661 gcagcgatta accgcttact tgacggttgt tgggttgact gccccgtaat cgttgagggt
     3721 gagcaaaaga acgaatcaaa gcagacaacc gaatgggagt ccaaagttga acgttttatg
     3781 aaacgctact ggtcggcaat caaagaggca gatagacgaa accttgtcgg taactattcg
     3841 gcgttattac ttcaagtaag agatggtcaa caatgggatc agcctatttt gcaaggcgcg
     3901 ctatctagca ttggagagtt cggactggtt aaactgatcc ccgtgtggca gtcacagctt
     3961 tcagtgacag agtttcaaga ggatgtgacc gccgataatt acggcgaacc gctgatgtat
     4021 caattcagcg aatctgcctt tggcaaaaaa agcgtgtcac gaaatatcaa ggttcacgca
     4081 agtcgtgtaa ttttgctcaa tgagggtggc gactttaatt cccctcattc tggagttcct
     4141 ctgcttgagg cgggttacaa caagctagtt gatctcgaaa aaacatcggg cggcagcgcc
     4201 gaaggttttc tgaaaaacgc aagccgacaa ttagggatca aattaacaaa agatgttgat
     4261 ttgagacagc tcgaagacag cgcaaaagcc cttgggtttt cttcattttc tgatgcgttg
     4321 aacgacaaga tcaagaaaat caactcagga acagactcag ccttgattac tcacgaaggt
     4381 gatgcctctg tgttgtcagt cgcaccagca gatccgaagc caacgtggga aatttctgca
     4441 aatgagtttg ccgcctcagt acaaatccct ttcacgattt tgttcggaca gcaaacaggg
     4501 cgattggcaa gtgacgagga caaaaccgac tgggcaaacc gttgcaacgg tcgtcgaaat
     4561 ggttttctca ccgatgtgat aacacaacta cttaatcgct tgtggttcat cggtgtgcta
     4621 cctatgccaa aaaatagcga tgtcacagtg tcttggtctg acttacttgc accaagcgaa
     4681 aaagagaaaa tcgcaaacgc acaggctttg gcttcagttg caactacatc gcagtctgcc
     4741 tttggttttg ccgcggtaac agcgaacgaa atccgcgaat ctttagggtt tgaacctctg
     4801 cctgataatc tgattccgcc aacaattgac gacgaaaatg aagatcaaga cgaaaatcaa
     4861 gccactgcgg attccgacaa ataaagccga tccgctcaat atgggagtgt cggttgccaa
     4921 gctatttcgt cacattgatg tcatttactc acaaattaag cgctccatta agcaacaaat
     4981 cttgcaaaag atgtcactgc gactgcaagc aaataatttt gagtattttc cttcgtatct
     5041 tactgcgact gagttggcgg atttgcttga aaccttgcaa aagattatcg acaaagagat
     5101 tttgtcgaat ggttctcaag gcgaaaatct ctggtttgac tcctatatcg acgaagcagc
     5161 actaaaaggc actcagtctg cggtcactga tttaggtctg caatcagagg cttatcgcag
     5221 tcagcgaaac ttatcatcaa tcgttttcag tcctgcgtac ttcaatcgat tggcgattgc
     5281 acacatggca agttatagcg agtggcaagg tttaagcgac gagttgcgaa aatctttggc
     5341 gggggtgatc acggaggcgg ttttaaacgg caaaaacgtg aaagaaactg cccgtgagat
     5401 tcggcaaaaa cttgatgtgt catcaaaacg ggcgaagcga atagcgcaat caagccagtt
     5461 ggcggcatat cgtcgggcag aatggcaaga ggcggaagag gcaaaagaag aacttgggct
     5521 aaacaccaag ctcttacatt tttcagcgct caaagctact acgcgattag cacacgccca
     5581 acgccacggc aaatacttcg atgttgcaga ggtgcgcgaa tggtatagcc gagacggcaa
     5641 ccaattcaac tgttactgca agcagtctgt ggttgtggtc aatgccgacg gtaaaacaga
     5701 tattgagcct ctccttgttg ggttggctaa agaacgtaaa caatgggtag gagcattaaa
     5761 aaatgtcaaa aaataacgtg aacatcgtca ccgtcattaa cagcaaaaat atcagcacag
     5821 aatctattga cggagatcaa cacctgatta tccgcggcgt tgtgccgatt gttgatgatg
     5881 ttgtgatgaa cgacggactt tatcctgcgg atgaaatcaa taaaagctac aacagcttag
     5941 agggcaactt tatgccgctt gggcatccaa aaatcgaagg taaatacgtt tcagcccaag
     6001 atgttcgagc ggtgaacaaa catcacgtcg gtgcgtgggc gaagaacgta cgcaaggaaa
     6061 gtggcaaagt gctggtcgat atgtatatca accgccgctt tgcggaaggt tcagaacagg
     6121 gcaaaacctt gcttgaacga cttgacggaa tggtgacaaa caccaacgct gaacctattc
     6181 acgtttccac agggctgatt ctaaacaaag tcaagcaatc gggtaaatcc aaaggcaaga
     6241 aatatagctg gattgcgaca aatatgtcgt tcgatcatat tgcaatattg cttgatgtgc
     6301 cgggcgcggc gacacctgat gatggcgtgg ggatttttgt aaaccaagac ggaagcgaaa
     6361 tgggggtgga aaatgtaaac ctaacacaag cggcggatta ccgcacagaa aagctgtttg
     6421 acaaagtacg attttacctg atggcgaact cagatttatc gtttgaagaa attcatcgcg
     6481 cgctgcgtga agctattcgc cccgaaggca aagataagtg gtcattttgg gtcgaagctg
     6541 tttacccgaa atacttcatc tattcggacg ataacagcgg caagaaatac aagcggagct
     6601 attttattga cgaaaatgac aatgtgcaac tcactagcga acctgtcgaa gttgtcaaga
     6661 agattggcta cgaagaaatt acaaccaatg aggaacataa tccaatgaaa accaaaatct
     6721 tagccgtgtt gaatgcggca aacgtgaaaa cggaaggctt aaatgacgat cagctcttgg
     6781 aagcctacaa caagctgcaa gcggataagg gtaaagagcc tgaaaagcct aaaaatgaag
     6841 atgacagcga aattgatgag aaaatcaaga aagcagtcaa tgccgcgatt gagccactcc
     6901 agcaagcctt gcaagctaat actgacaaag agcaagcagc aatgcgtgcg gcggtgaaag
     6961 ccaagttcaa tatgagcgat gccgcagtca atgctttgca aggcgaggta ttaactgagt
     7021 tgtatgcgca aacgcaacaa tcggtcggat taaacaataa ttcggccaac ggcaacggcg
     7081 acaatcaatg ggatggttac tcactcaacc aagaggagaa aggcaatgag taatgtaatt
     7141 tatcgcggtt cggtcaagcg tgaacctcaa acggtgaata tcgtgatcaa cgatacttcg
     7201 gcaccgggtg cagtagttaa actccacaac ggcaaattag aggcggcggc tgatttaaaa
     7261 ggtcgccgtt ttttattggg caacaaccgc tttaaggggc agacaatcga acaagcgtat
     7321 gcgaagggcg atactgcaac ggcgtttcgt ttagagcctg aacaggagta ttacgcgcaa
     7381 attgccgacg gcacttataa ctttggcgat gaactcacgg taaaacaagc gggcggcaaa
     7441 ctgaccaaag cggagacggg cgatgtagtg ctatttttct tcgacgaaga atcgcagcgt
     7501 gtaatcagtg gcggcaaagg ctatgccgat gtcgtggtgg caaacgccta cactaaataa
     7561 gaggtaacaa agcaatgttg aaattcacaa aagaacaaga acgcttcgtg attaacgagc
     7621 ggatcaatta cgacaaaaaa cacgcgctaa tggcagcaaa ttcggcgggg ttattgggca
     7681 acgcgatgac tttgccgaaa gatgtctggg cggaatggga tcgtgatgca gtgcaaattc
     7741 agcgcgaaga attggtggtg tttaacgatt tagcgggcat ttcaaaatca atgccgattg
     7801 gcaaacttat tcaccatttc caaacggtat ctgatagcgg ttcggttaat atctcattag
     7861 acggtcgttc atcagcgaaa accgataacg tggtgcttaa ctaccacggt acaccgttgc
     7921 cgattattga ttccactttc tcttttggct ggcgcgatat gtcggcagct caaagcgaag
     7981 ggtatcagat cgacggtgca gcacgcgcga acagtctccg caaggtggct gaaaaaatgg
     8041 aagatctcgc tttaaatggt gattcctcta ttgtggttgg caatgccaag ctttacggct
     8101 tgcgtactgc gccaaatcgt atgactaaca cacacaatct ggatttagcc actgcgacac
     8161 cgaaagaaat ttacgatgtg ttccgtgact tgattagcaa attccacgcg aagaactact
     8221 actctcctgt aacgctttat gtgaactatg gcgactattt cgcaatgagt acacgtgatt
     8281 actccgagca gaaatccgaa ggatctatct tgaacaaagt gatgacgatt ccgcaaattg
     8341 cgaaaatcgt gccagcaagc cgtgtaccgc aaaatgaaat tctcggcttg tgcaagcgtt
     8401 cggatgtgta ccaagtgctt aacggtatgc cgttagtgac tcgtccgatt gcgcgccata
     8461 atgaaaccga tgattactcg ttccaaatta tggcagccgt tgccgtggag ttcaaattcg
     8521 acgataaagg caatgctggc tatatccagt acactaaatc ctaaggcggt gcaaaatgaa
     8581 gtggctatta acacacgata gccacgagtt gaagaaaggc gatgtttacg aaggcgaaac
     8641 attaccgctt tggcttgtgg gcaaagctat cgaaatcgaa gaaaaatcct ttgaagttgc
     8701 aacgccttct gatcacgctg acggcgaaac tggcgaaact ggcgaaactg gcgaaactgg
     8761 cgaaactggc gaaactggcg aaactggcga aactggcgaa actggtcaga aaggcaaaaa
     8821 agataaaggc aaataactat ggcagcagaa atagagctat ccgaagcgcg aacaacgctt
     8881 gacgaactgg gtttcacttc gccgaactct ctgcttgagc tttacgttca gcaaacgaac
     8941 accttagacc aggcgttgaa caaatcgggg tacagtgaag aaacacagaa actgatcaag
     9001 ctctatctgg ttgctattct tgcaatttca tctggggcgc gccgtataaa gtcagaaggt
     9061 gcgcctagcg gtgcaagccg aacgtttgag tatgatccaa acgcgctaga caaccttaag
     9121 tcggcgattc gctcaattga tccgaaaggt tgcgctgaaa gtttgctacc caaagataaa
     9181 gttgtcggct ttttcgatgt ggtcggaggc tgctaatgtc aaatttatcg aattggtcct
     9241 acaccgcaaa agccacgctt tggcaggcta aaggaaagaa cgatgacggc gttttagttt
     9301 tttctacgcc acaaatcatt tcctgtgact acggtgcaga tagaaagcgc gcacgttttg
     9361 agattgggcg tgaacaagcg gtcaaaaatg tgatctggac ggagttcgcc cacgccaaat
     9421 taggcgatta tgttctgatt ggcgaaagcg agcaagccga tccgattttg gctggcgcag
     9481 aggaaattat tcatatacag cgctttgctg atacgttcga ccgcaatcgt gatgattttg
     9541 cgctgataac aggcgggtaa aatggggatc aaagtaaaag gcataagaca ggtcaagcag
     9601 cggctgaatc aggttgttgg cgaggttcag agccaaaaag caactcgggc aatgtttcgc
     9661 attttgaatg cggttgcccc tcttgcggcg cactatacgc ctgttgatac ctcaacgttg
     9721 atcaacagtc aattcactga aatggaagtg aacggcacac gcttgattgg aagagtgggt
     9781 tattctgcta attatgcggc ttacgttcac gatcctaata tcaagcaaaa cttccgcaag
     9841 ccttcagcga agaaagagtt tttgacttcc gcgctgaacg aatcaaaggc tacgatcaaa
     9901 tccattgtta aagaggagtt gtcactatga tttcttatgt caaagccttc aaacaatggt
     9961 tagaggctca ctctcttgca gacggctatg ttgtgcagct ttaccagtgg gaggatacgc
    10021 aaaaagccaa gcctgttatt gttattcagc caaatagtgg ttcgccgcaa gtttctgacc
    10081 tgagcagcga acactatctg ctaattagcc ttgttgcagg taaaaattca ggttacacaa
    10141 tcgaagagcg agcaagggcg attatgtcaa agattttagt tgagcctttc gcttcctttg
    10201 gttacatcga atcaatgggc ggattacccg ccccaatttt caccgaagat aatcgaatga
    10261 tttttcgctt accacttcgg attatttcaa caaatcaaga ggagtagttt atgggttctc
    10321 catctccaca aaataacgca gaaaatctcg tggtggggcg tgccgtagtt ttagagtacg
    10381 gtacgactga tgcaaagcca caagaaagcg aatggaaggt tgcgggcgca atgacgacaa
    10441 agagttggga ttttagccca aattcggtta catcagaggc tgatgatgct ggcggcttcc
    10501 ctgaaacctt aattacaaac tcagacttta gtatttctgg tgacggtgag tggcgcaaac
    10561 gaccaaaatc gaaagaacta ggcatcaaag atctagttgt gttatacgca aatgcggtaa
    10621 aaaatcgcac ccaaccttac atttgggttc gtttgaaata cgcaggctta acaattctcg
    10681 gaaaaatggt tatcactgca ttaagtagcg aagccccaac caatgactta gtgaaattct
    10741 ctatagagtt caaggttggc gattcaagca ctatcgaaat tgctgcagcg taaaaaatcc
    10801 caccctctat aacaagagcg gtgggattat cattatccta gggatagttg taggggcttt
    10861 cctaagctag ccaaaatacg accaatagtg tcgatttttg taatatggcg aggggctaaa
    10921 attcgctgta cttctggcgg acggatatta gttaatcggg caatttctgc cttgcttacc
    10981 gcttgttctt gcattgtatt atgcaaaagc acttttgcgt agatgctctc tggcataaac
    11041 acaggcactt cgtcggtatt tactgggcga gtgagcggaa agcgtctatc ttcatcgaaa
    11101 taaatttcaa cacaagatag tagcacgtct tctgccattt ccattgcttc ttcaaatgta
    11161 tcgccttggg tgatagcttc tggcaaatca ggaaatgtca ctacaaagcc cccttcttca
    11221 gcgggggtaa ataacgctgg ataaaacata ttttctcctt attcaagggt gtagtgaaag
    11281 ccccttaaca tcaggggctt tatattattt taagcctagt tgctttttaa ccccttccac
    11341 taagcctgtt tttaactctt ggcttgggtg tttggggagg tggcttcttt tgcccttgta
    11401 gtagagtttc aaatgctttg atccattttc aacctctacc ccattagcct ttagccacct
    11461 taggaattcg ctttgcttca ctacctcctc ctatttgttt aacttgggtt cattatatgc
    11521 aaaaatgcta actttgtcaa cagaaagtta gcaaaaatgc taattttttt gttgaggtga
    11581 aaatggttag ctatccaatt actgaaattg gtgaaatgct tattgcaacc gatgggcgag
    11641 actttttatt cagaccgagt ttcaaaaata tctacaaaat tggaagccca caagaaattg
    11701 taaaaatcta tgcactattg cacggctgtg agattcgaga gatattgccg cacattttaa
    11761 ccaaatcgga gtctcatcaa tcttactttt ggtcagtgct gaattcgcct gtttttggca
    11821 gaaaaattct gcaaaacgca atgatcgtga tgtcgtgctg ttgcgatgat gatatttccc
    11881 tgttgattgg cgagtggttg ccgtcaaagt ctggcgttag gtacaagttg ggtaaaatgc
    11941 cacctgagac aattatcaat tttgcaaaaa atttaatggg tcacggagtg attggttgct
    12001 gcgaattgcc aaagaaagaa caatctgcaa aaaatgaatt ttcagaaagt tttgatgctg
    12061 ttgagtacat aagtttagcc cgaactcatt tcaatatgag ccgcgaagac gctgaaaatc
    12121 tcacgatgac cgaattgcag caacttctaa aaagtcaaat gcctgccgaa aataatagct
    12181 atggacagtt caccgaagaa gagtacgaca gaattatggc ggaatatgag aaaagaagag
    12241 cggcttaaat agccgctaac atttaaaaga acctacctga tagaacggga gtgaaatcct
    12301 tgtaatgtgc tgaataaaaa agctctctta tgtcattgag tagctcaaat tcatctttgg
    12361 atcttgcgtt gcagattgcc acatagtcat ctacgcagga taaaatacgt tcaatactga
    12421 cttcttttat ccagcatact tcttggtttt cattcagcca gttggcaaag tcttgtttag
    12481 tgcagtcttt tttcataagt tctccttttt gattatttgc aaaaaaaatc atatcaaatc
    12541 atccgttgtt atctattggc gaaaataatt tttgcgacac agatcgcaaa aataggtttt
    12601 gacggtggaa aa
//
LOCUS       DANLXF010000059        12526 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.059, whole genome shotgun sequence.
ACCESSION   DANLXF010000059 DANLXF010000000
VERSION     DANLXF010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12526
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.059"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(313..495)
                     /locus_tag="PXD66_001057"
     CDS             complement(313..495)
                     /locus_tag="PXD66_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319368.1"
                     /translation="MYQFGYLRTKIGTDTQRAMLWNCPTYVLIIPFLLNTSVKKQYHA
                     FWQKYHQHNNDVSFPY"
     gene            645..1685
                     /gene="selD"
                     /locus_tag="PXD66_001058"
     CDS             645..1685
                     /gene="selD"
                     /locus_tag="PXD66_001058"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020909986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:690..692,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HDL6319369.1"
                     /translation="MSEPIKLTQFSHGAGUGCKISPKVLGTILQTQLEQFVDPNLLVG
                     NETADDAAVYDLGNGQAIISTTDFFMPIVDAPFDFGRIAAANAISDIYAMGGKPIMAI
                     AILGFPVNKLPAEVAQQIVEGGRFACQQAGIALAGGHSIDSPDPIFGLAVTGIVPTER
                     VKRNASAVAGCELFLTKPLGIGILTTAEKKGLLKPEHQHLASEVMCQINLIGSAFSEL
                     PEVTAMTDVTGFGLLGHLSEVCQGSGLRAEVDFEDIQVLDGTKEYIALGAVPGGTNRN
                     FDSYGHLISAMTDEQKAILCDPQTSGGLLVAVLPQAVEKVQQIANRANVPLFHVGRLL
                     EMEQGKPRIEVI"
     gene            1685..2158
                     /locus_tag="PXD66_001059"
     CDS             1685..2158
                     /locus_tag="PXD66_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL6319370.1"
                     /translation="MFKPNVTMACVVHCKGKFLFVEEIEYGKRTLNQPAGHLEKDETL
                     LEGASRELFEETGIRAEMANLVKIYQWQAPRSQTDFLRFLFSVELEEWLEPNPQDADI
                     TQALWLTLAEFEDYIQQQGQCARSPLVVQSVKDYLSGERYPLSLLNAFEWARNGK"
     gene            2148..3356
                     /gene="ubiH"
                     /locus_tag="PXD66_001060"
                     /gene_synonym="visB"
     CDS             2148..3356
                     /gene="ubiH"
                     /locus_tag="PXD66_001060"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HDL6319371.1"
                     /translation="MENKTTTQFDVVIVGGAVTGSVLALALSSFTHHKISIAIVEKSL
                     PDYEQQGGFDARSIALAEGSLQKMGQIRPLAGSSLGEMISEIGTPIQQIQVADKGHFG
                     KTTLKASEQNVAQLGMVVELAKFGRKLTACLAQQANITLFCPNQVVGIERSQQHCGLS
                     LKNGEKLSCSLLVAADGIQSQIAKQCGVETVKVRDYAQSAIIANVELSESHQNQAFEY
                     FTPQGPFALLPLSGNMMSLVWCVKEPNELIQLSDSEFLARLQQQFGWKLGQFVRVSKR
                     FVYPLILQKAQSHIHHRLAVVGNAAQMLHPVAGQGFNLGLRDLTTLAELVALAFNQGK
                     DIGEFSLLSTFENERTQDQARMINATSGLISLFCCEMLPVQVVRNLGLLTVSHSKIAR
                     DFVANQALGW"
     gene            3619..4851
                     /locus_tag="PXD66_001061"
     CDS             3619..4851
                     /locus_tag="PXD66_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease"
                     /protein_id="HDL6319372.1"
                     /translation="MTTENMSTPKQAFIGLQMLFVAFGALVLVPLITGLDPNVALLTA
                     GVGTLIFQICTKRQVPVFLASSFAFIAPIQFGVQQWGIPVTMGALLCSGFIYMALSAL
                     VKAKGVNILHKYFPPIVIGPVIMVIGLGLAPVAVDMATGKSANIAYNTAIIVSMITLV
                     TTLIVAVFAKGLLKLVPILAGLTVGYILSVFMGLVDFTKVVEAPWFSLPKVSAPEFKL
                     DAILYLLPIALAPAVEHLGDVMAISQVAGKDFMKKPGLHRTLLGDGLATSAAAALGGP
                     PNTTYSEVTGAVMLTKNFNPKIMTWAACWAIAFSFCGKIGAFLATIPTFVMGGIMMLL
                     FGAVAVVGINTLIKAKVDLSIPRNLCIASVVMTFGIGGMLINIGEFSLKGISLCALVA
                     IVLNIVLPKEKVEDSAAH"
     gene            4918..5904
                     /locus_tag="PXD66_001062"
     CDS             4918..5904
                     /locus_tag="PXD66_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDL6319373.1"
                     /translation="MQKLTFSPPLLNTPCPWCSELESLRELYACEYTGAVTTRTSMPQ
                     PYPHDWAKNQYVLFDSNAQKTASVNTQDATSLQTASLNTIGLSPNNLDTTISFVRTIS
                     NELTAPSSKPFIISVFGSPEEVGECYEKIVAFQTEVKMPLAMEINISCPNIPGEISPA
                     YSAEELSHYLHALQTSLKKLAQGDNTLPIGIKIPPFTYQNQYDELIKGLLQAVENGEN
                     LPISFITSTNTLGSSLVLNAGKAVLNSEVGTGVGGMAGTAIHALSLGNVYTIRQMLDQ
                     EPKLKHLQIIGVGGVNDKAGFERMKSVGADFVGLATALGVKGIGVFKEILSE"
     gene            complement(5945..6355)
                     /locus_tag="PXD66_001063"
     CDS             complement(5945..6355)
                     /locus_tag="PXD66_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HDL6319374.1"
                     /translation="MLYSVLTAHILLNEPYRNGAHSTQHEHLVVMFEIDEESGYFYAM
                     DLHQTEQPVVDSLFVFRVSDIEQNSLKEPRRLEICWSEDGYQAFLLINGYPHAVFDFR
                     QFVGYNHTKFPMPELGSMWVHKETTNELVEKWLG"
     gene            complement(6421..7731)
                     /gene="eno"
                     /locus_tag="PXD66_001064"
     CDS             complement(6421..7731)
                     /gene="eno"
                     /locus_tag="PXD66_001064"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDL6319375.1"
                     /translation="MAKIVKVIGREIIDSRGNPTVEAEVHLEGGFVGLAAAPSGASTG
                     SREALELRDGDKGRFLGKGVLKAVSAVNNEIANALVGKDGSAQAEIDQIMIDLDGTEN
                     KSKFGANAILAVSLATAKAAAASKGLPLYAYIAELNGTPGVYSMPLPMMNIINGGEHA
                     DNNVDIQEFMIQPVGASTLKEALRIGAEVFHNLAKVLKAKGLNTAVGDEGGFAPNLAS
                     NADALACIKEAVEKAGYVLGKDVTLAMDCASSEFYDKERNVYDMKGEGKTFTSQEFTH
                     YLEGLCKEYPIVSIEDGQDESDWEGFAYQTKVLGDKVQLVGDDLFVTNTKILKNGIEK
                     GIANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQ
                     IKTGSMSRSDRVAKYNQLIRIEEALERAGTPAPFNGRKEIKGQA"
     gene            complement(7922..9100)
                     /gene="rlmC"
                     /locus_tag="PXD66_001065"
     CDS             complement(7922..9100)
                     /gene="rlmC"
                     /locus_tag="PXD66_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HDL6319376.1"
                     /translation="MSSLLHCSHFERGDCTSCQWIEKPYSTQLAAKEADLKRLILPFI
                     LQNKTEILPLVGSPISQFRNKAKMVVSGSVERPILGILKDQNDAQSGVDLTDCLLYPS
                     SFHRLFPILKDFIARAGLVPYNISKKKGELKYILITESQYNQSFMLRFVVRSEAKRAL
                     VERELPELLEKLPPNSVVSLNIQPQHAALLEGETEIFLTEQKVLEEHFNGIPLFIRPQ
                     GFFQTNPVVASQLYHTAQSWIEHLPIAKLWDLFCGVGGFGLHCANALQAKNSNVELTG
                     IEISASAIASATKSAEKLGLKQVKFASLDSAQFALNEAGSPPDLVIVNPPRRGIGKLL
                     AEFLNQLGSPYLIYSSCNAVSMANDFTFLTNYELHKVQLFDMFAHTAHYETLTFLVKK
                     "
     gene            complement(9093..10130)
                     /gene="nagZ"
                     /locus_tag="PXD66_001066"
     CDS             complement(9093..10130)
                     /gene="nagZ"
                     /locus_tag="PXD66_001066"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HDL6319377.1"
                     /translation="MLLIDIKDKELTQEEVEMLEHPLVSGLILFTRNFYDKAQIQALV
                     KSIRQRVKKPLLITVDQEGGRVQRFREGFTKLPAMQSFNQLATTPEQAMQLAKETGWL
                     MAAEMFALDIDLSFAPVLDLGHECKAIGDRSFGQEPAAILPVAEAFIDGMREIGMATT
                     GKHFPGHGHVLADSHLETPFDDRPKERIFNHDILPFKQLIAKGKLSAIMPAHVVYTQC
                     DSQPASGSSYWLKEVLRKQLNFNGVIFSDDLGMKGAGFMGNFVERSEKAIQAGCDLLL
                     LCNEPEGLVQVLDGLKYQPTQAQKERRLSLMKRKTVTWRELENSSRYQQTQAELTALQ
                     NNWLEWKEANV"
     gene            complement(10256..10987)
                     /locus_tag="PXD66_001067"
     CDS             complement(10256..10987)
                     /locus_tag="PXD66_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDL6319378.1"
                     /translation="MFDWITNPEAWVALFTLTGLEIVLGIDNIIVISILVSRLPIEQR
                     QSARIIGLGLAMGTRILLLLSLAWMMRLVEPLFTIGNNPISGRDLILLIGGLFLIVKS
                     AMELKESISAESHEEKSESIKKASFFMVLIQIAIFDIIFSLDSVITAVAMADDIPVMV
                     IAIIIAVAVMMLAAKTIGDFVDNNPTIKNLALAFLILIGIVLVGEGFDIHIPKAAVYT
                     AMGFSVVVEMLNIKMRRNQAKLEQA"
     gene            complement(11076..11756)
                     /gene="mutH"
                     /locus_tag="PXD66_001068"
     CDS             complement(11076..11756)
                     /gene="mutH"
                     /locus_tag="PXD66_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HDL6319379.1"
                     /translation="MQLSTFSPTEQALLSKAEWLAGFTLGEIAEMLHIPIPADLKRDK
                     GWVGTLIETALGAKAGSKAEQDFAHLGIELKTIPVNQKGLPLETTFVSLAPLTQNNGI
                     IWETSHVKHKLSRVLWIPVEGERKIPLAERHIGQPILWSPTITQESRLQQDWEELMEL
                     IVLGELHKINATLGEVLQLRPKGRNNRSITSAINAKGEIVQSLPLGFYLRKHFTAEIL
                     QNFLHCPL"
     gene            complement(11848..12483)
                     /gene="lexA"
                     /locus_tag="PXD66_001069"
     CDS             complement(11848..12483)
                     /gene="lexA"
                     /locus_tag="PXD66_001069"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HDL6319380.1"
                     /translation="MRKHLTARQQEIFDFVKNHIETTGMPPTRVEIAREIGFKSPNAA
                     EEHLKALARKGYIEMLSGTSRGIRILVEDENQAANDEDEGLPLIGKVAAGTPILAVEH
                     VENHYPVNGAMFNPSADYLLKVNGNSMEKIGILDGDLLAVHKTNTARNGQVVVARVND
                     EVTVKRLEKKGELIYLHPENDELKPIVVNPAQEYIEIEGIAVGVIRSNAWM"
BASE COUNT         3508 a         2544 c         2797 g         3677 t
ORIGIN      
        1 aggaaaaatg tgctgtggca gaacaccgat ggcaacctta cttgatggaa aacggatttg
       61 ggtggaaaag aatttaagct caaattaatc tgacagacac ggtaatttaa aacgggagac
      121 tgtcagatca agtctgatct tctacaacta agccttctca gaataaataa attttcacaa
      181 gcggtgcaac tttgcaaaaa cttcggtatt atgtagcagt tgcacaaaac cccaaattca
      241 ttaaaaccgc ttaaaaatga aatgccctaa actgattttt cagtctaggg catttattat
      301 cacatattat ggttaatagg gaaaactcac atcattgtta tgctggtgat atttttgcca
      361 aaatgcgtga tattgttttt tcaccgatgt gtttaataaa aacgggatta tcaagacata
      421 ggtcgggcaa ttccatagca ttgcacgttg tgtatccgtg ccgattttgg tgcgtaagta
      481 accaaactga tacattagac ccaatccaac cgtcacctat tctaatagca taataactaa
      541 ggtcgcatca ttaaattaaa agctcatttt ttgcaaaaaa tttcccaaat tccaccgctt
      601 gttggcgtac aataggcata attttgattt acgatggagg cagaatgtct gaaccgataa
      661 aacttacgca gttcagccac ggggcgggtt gaggctgtaa aatttcgcct aaggtgttag
      721 ggacaatttt acaaactcaa ttagaacaat ttgtcgatcc gaatttattg gtaggcaatg
      781 aaacggcgga cgatgctgcc gtctatgatt tagggaacgg gcaggccatt atcagcacca
      841 ccgatttctt tatgccgatt gtggacgccc cgtttgattt cgggcgaatt gctgctgcca
      901 atgcgattag cgatatttat gcgatgggtg gcaagccgat tatggcgatt gcgattctgg
      961 gctttccggt gaataaattg ccggctgaag tggctcaaca aattgtcgaa ggtgggcgtt
     1021 ttgcgtgcca gcaagccggt attgccttgg cgggcgggca ttcgattgat tcgcccgatc
     1081 cgattttcgg cttggctgtt accggcattg tgccgaccga acgggtgaaa cgcaatgcct
     1141 ctgcggtggc tgggtgtgag ctgtttttaa ccaagccttt ggggattggc attctaacca
     1201 cggcagagaa aaaaggtttg ttgaagcctg aacatcaaca tctggcaagc gaggtaatgt
     1261 gccaaatcaa cttgattggt tcagcgtttt ccgaactgcc tgaagtcacg gcaatgactg
     1321 atgtaaccgg ttttggctta ctcgggcatt taagcgaggt gtgtcaaggc tcgggcttac
     1381 gggcggaggt tgattttgag gatattcaag tccttgatgg cactaaggag tatattgcat
     1441 tgggagcagt tccgggtggg acaaatcgca atttcgacag ctatgggcat ttgatttcgg
     1501 caatgacgga cgagcaaaaa gcgattttgt gcgatccgca aacctctggc ggtttattag
     1561 tggcggtgct gccacaagcg gtcgaaaaag tgcaacaaat tgcaaatcgg gcaaatgtgc
     1621 cgttattcca cgttggcaga ttgctggaaa tggaacaggg caaaccacgg attgaggtaa
     1681 tttgatgttt aaaccaaatg taacaatggc ttgcgtggtg cattgtaagg gaaagtttct
     1741 gtttgtggaa gaaattgaat atggcaaacg tacccttaac cagccggcag ggcatttaga
     1801 aaaagatgaa accttgctgg agggggcaag ccgtgagctg tttgaggaaa ccggtattcg
     1861 ggcagaaatg gcaaatttgg tgaaaattta tcaatggcaa gcccctcgtt cacaaaccga
     1921 tttcttgcgt tttctgtttt cggtggaact ggaagagtgg ctggagccga atccgcaaga
     1981 tgcggatatt acccaagccc tttggcttac tttagcagaa tttgaagact acattcagca
     2041 acaagggcaa tgtgccagaa gcccgttggt ggttcaatcg gtcaaagatt atttaagcgg
     2101 tgagcgttat ccattaagtt tgttgaatgc gtttgaatgg gcgagaaatg gaaaataaaa
     2161 caacaaccca atttgatgtg gtgattgtgg gcggggcagt gaccggctcg gtgctggcat
     2221 tggcgttaag tagttttact catcacaaaa tctcgattgc gatagtggaa aaatcgctgc
     2281 cggactatga acagcaaggc ggttttgatg ctcgcagtat tgccttagcc gaaggcagtt
     2341 tgcaaaaaat ggggcaaatc agaccgcttg caggctcaag tttaggcgag atgattagcg
     2401 aaatcggcac gccgattcag caaattcaag tagcggataa agggcatttt ggtaaaacga
     2461 ccttgaaagc aagcgagcag aatgtggctc aactgggtat ggtggtggaa cttgcaaaat
     2521 tcggcagaaa attgaccgct tgtcttgcac agcaagctaa tattacgctt ttttgcccga
     2581 atcaagtggt tggcattgag cgttcccaac aacattgtgg attgagcttg aaaaatggtg
     2641 agaaactaag ctgttccttg ctggtggcgg cagatggtat tcaatctcag attgcaaaac
     2701 agtgcggtgt ggaaaccgta aaagtaaggg attatgcaca atcggcaatt atcgccaatg
     2761 tggaactgag tgaatctcac caaaatcaag cctttgaata ttttacgcct caaggccctt
     2821 ttgctttatt gcctttgagc ggaaatatga tgtcgttggt gtggtgtgtg aaagagccga
     2881 acgagttaat ccaactttca gacagtgaat ttttagcccg tttgcaacaa caatttggtt
     2941 ggaagttagg gcaatttgtt cgagtgagca agcggtttgt ttatccgctc attttgcaaa
     3001 aagcccagtc gcatattcac caccgcttag cagttgtcgg caatgccgca caaatgcttc
     3061 acccggttgc tgggcaaggt ttcaacttag gcttgcgtga tttgactacc ttagcggaat
     3121 tagtggcttt ggcgtttaat caagggaaag atataggcga attttcgctc ttatcaactt
     3181 ttgagaatga gagaacgcaa gatcaagcca gaatgataaa tgctacaagc ggtctgattt
     3241 cgcttttttg ttgcgaaatg ttaccagttc aggttgtgag gaatttaggg ttattaacgg
     3301 tttcccattc aaaaatagcc agagattttg ttgctaatca ggcattaggt tggtaaaaaa
     3361 cacaaaagca gcaattttct atttgacact gtttttaaaa aggcgtatta tccaacccgt
     3421 ttaaaaatct caccaaagcc ttgaatgcaa attcaaggct ttttcctttt tagtgaaagc
     3481 ctatgaattt tgtttgccgt aaaatcggca aaaaaatttt gcctaaaatt cgcaatcgtt
     3541 tgcgaaatag gtttatagcg ttgttttcat ctgggtaact gcaatggtta catcgcaata
     3601 catcaaagag agtaaaaaat gaccacagaa aatatgagta cgccaaagca ggcgtttatc
     3661 gggctgcaaa tgctgtttgt tgcatttggg gcgttagtat tagttccgct gattaccggt
     3721 ttagatccta atgttgccct tcttaccgcc ggtgtcggta cgttaatctt ccaaatttgt
     3781 actaaacgtc aagttcctgt tttcctcgca tcgagtttcg cttttatcgc tccaatccaa
     3841 ttcggtgttc aacaatgggg aattcccgtc acaatgggag cattgctctg ttccggtttt
     3901 atttatatgg cattatctgc tttagtgaaa gcgaaagggg tgaatattct gcataaatac
     3961 ttccctccaa tcgtgattgg accggtaatt atggtgattg gtttaggtct tgctcctgtt
     4021 gcggtggata tggcaacggg taaatcggcg aatattgcct ataacaccgc tattattgtt
     4081 tcaatgataa cgctggtaac tacgctgatt gtggcggtgt ttgcgaaagg gctattaaag
     4141 ctcgtgccaa tattagcagg tttaacggtg ggatatatct tgtctgtttt tatgggattg
     4201 gtggatttta ctaaagtggt tgaagctcct tggtttagtc taccgaaagt ttccgcaccg
     4261 gaattcaaat tagatgcaat cttgtattta ttacctatcg cacttgctcc ggcagtcgaa
     4321 cacttaggcg atgtaatggc aatcagccaa gttgccggta aagattttat gaaaaaaccg
     4381 gggttacacc gcacgctttt aggtgatggt ttagcaactt cagcagctgc ggcattaggt
     4441 ggcccaccaa atacgactta ctctgaagta accggtgcgg taatgctaac caaaaacttc
     4501 aatccgaaaa ttatgacttg ggctgcgtgc tgggctatcg ctttctcatt ctgcggaaaa
     4561 atcggggcat tccttgccac tatccctact tttgtgatgg gcggtattat gatgttgctg
     4621 ttcggtgcag ttgcggttgt ggggatcaat actctaatta aagctaaagt tgatttaagc
     4681 attccacgta atctttgtat tgcttctgta gtaatgacat tcggtattgg cggtatgtta
     4741 attaacatcg gcgaattctc attaaaaggg atcagcttgt gtgcattagt cgctattgta
     4801 cttaacattg tattaccaaa agaaaaagta gaagattctg ctgctcatta atcaaaggac
     4861 gctgtggcgt cctttttgct aataaccctc tatttgtaaa taaaaaggaa taacactatg
     4921 caaaaactca cattttcacc accactttta aatacgcctt gcccttggtg ttcggagtta
     4981 gagagcttgc gtgaacttta tgcgtgtgaa tataccggtg cggtaacaac ccgtacttca
     5041 atgcctcaac catatccaca cgattgggcg aagaaccaat atgtactctt tgattccaac
     5101 gcacagaaaa cagcaagtgt gaatactcaa gatgcaacta gcttacaaac agcaagcctt
     5161 aacaccatcg gtttaagccc aaataatctg gataccacca tcagctttgt acgcaccatt
     5221 tcaaatgagc tgacagcgcc atcaagcaaa ccatttatta tttcggtgtt tggttcaccc
     5281 gaagaggtgg gggaatgtta tgaaaaaatt gttgctttcc aaactgaagt gaaaatgcct
     5341 ttggcaatgg agattaatat ctcttgcccg aatattccgg gtgaaatttc gcctgcttat
     5401 tccgcagaag aattatcaca ttacttgcac gcactacaaa cttcgctaaa aaaactggct
     5461 caaggtgata atactttacc aatcgggatt aaaattccac cttttactta ccaaaatcaa
     5521 tatgatgaat taatcaaagg cttattacaa gcggtcgaaa atggcgaaaa tttaccaatt
     5581 tcctttatta cttcaaccaa tacgcttggc tcatcgcttg tcttaaatgc tggcaaagcg
     5641 gtattaaatt cagaagtggg aacgggcgta ggcggaatgg caggaacggc aatccatgca
     5701 ctttctttgg ggaatgttta cacgattcgc caaatgttag atcaagagcc taagctcaaa
     5761 catcttcaaa ttattggtgt gggcggcgtg aatgataaag ccggctttga gcgaatgaaa
     5821 tcggttgggg cagactttgt ggggttagct accgcattag gggtgaaagg aatcggcgtt
     5881 tttaaagaga ttctgtctga ataatgaaga ggagggcgtg cattgcacgc ccgaataaat
     5941 gaaattagcc taaccatttc tccactaatt cattagtggt ttctttatgt acccacatac
     6001 tgccaagttc cggcattggg aatttggtgt ggttgtagcc gacaaattgg cggaaatcaa
     6061 acacggcgtg cgggtagccg ttgatcagta aaaaggcttg atagccgtct tcactccagc
     6121 aaatttctaa acggcgaggt tcttttaatg aattttgctc aatatcgctg acccgaaaca
     6181 caaataaact atccactacc ggctgttcgg tttggtgtaa gtccattgca taaaagtagc
     6241 cgctttcttc atcaatttca aacatcacta ctaaatgttc gtgctgggtt gaatgagcgc
     6301 cattacggta aggttcgttc agtagaatat gagcagttaa aacagagtag agcattaaat
     6361 tatccttata agtaaaatgc caccgatttc tcagtggcat ttaaatattt aatcaggcaa
     6421 ttacgcttga cctttaatct ctttacggcc gttgaacgga gccggtgtac cagcacgctc
     6481 taacgcttct tcgatacgga ttaactggtt gtatttcgct acacggtctg aacggctcat
     6541 tgaaccggtt ttgatttgac ctgctgctgt acctactgct aaatctgcaa tggttgcatc
     6601 ttcagtttcg cctgaacggt gagagataac tgcggtgtaa cctgcatctt tcgccatttt
     6661 gattgcagct aaagtttcag ttaaagaacc gatttggttg aatttgatta agattgagtt
     6721 tgcgatacct ttctcaatac cgtttttcaa gattttggta ttggttacga ataagtcatc
     6781 accaactaat tgtactttgt cgcctaatac tttagtttgg tatgcgaaac cttcccagtc
     6841 agattcgtct tgaccatctt cgatagacac gattgggtac tctttgcata agccttctaa
     6901 gtagtgagtg aactcttgtg aagtaaaggt tttgccttcg cctttcatat cgtacacatt
     6961 gcgttctttg tcgtagaatt cagatgatgc acagtccatc gctaaagtta cgtctttacc
     7021 taacacatag cctgcttttt ctaccgcttc tttaatgcac gctaatgcgt ctgcgtttga
     7081 agccaagttt ggtgcgaaac caccttcgtc acctacagca gtatttaagc ctttcgcttt
     7141 taatactttt gcaagattgt ggaatacttc agcaccgata cgaagagctt cttttaatgt
     7201 agaagcacct accggttgaa tcataaattc ttgaatgtcc acgttgttgt cagcgtgttc
     7261 accaccattg atgatgttca tcattggtaa tggcattgag taaacacccg gtgtgccgtt
     7321 taattcagca atgtatgcgt aaagcggtaa gccttttgaa gcggctgctg cttttgctgt
     7381 tgctaaagaa accgctaaga tagcgtttgc accgaatttt gatttgtttt ctgtgccatc
     7441 taaatcgatc ataatttggt cgatttcagc ttgtgcagaa ccgtctttgc ctactaatgc
     7501 gtttgcaatt tcgttattta cggcagaaac cgcttttaat acacctttac ctaagaaacg
     7561 acctttgtcg ccatcacgta attctaatgc ttcacgtgaa ccggtagaag cacctgatgg
     7621 agcggctgct aagcctacaa aaccaccttc taagtgaact tctgcttcta cagttgggtt
     7681 accacgagag tcgatgattt cacgaccgat tactttaaca attttagcca ttgttgatgt
     7741 cctctaatat acgagttaat agaaaaaaga ataggtatat agtaaagcga ttttctgctt
     7801 ttgtcttgga aaaactgatt gagtggcaga aaatttgcca ctaatttgat ctaagactat
     7861 attcgcctga gattatagca acctttgcaa aaaacggcaa aaatttgccc gcttgtaatg
     7921 cttatttttt gactaaaaaa gtcaaggttt cataatgtgc tgtgtgagca aacatatcaa
     7981 acagctgcac tttgtgtagt tcgtaatttg tcaaaaatgt aaaatcgttc gccattgaga
     8041 cggcattaca actggagtaa atcaaataag gtgagcctaa ttgatttaaa aattctgcta
     8101 atagtttgcc gataccacgt cgaggtgggt tcacaatcac taaatcgggt ggagaaccag
     8161 cttcatttaa agcaaattgt gcagaatcta atgaggcaaa ttttacctgc tttaggccga
     8221 gtttttcagc cgatttggtc gcacttgcaa tagctgaggc tgaaatctca atgcctgtca
     8281 gctccacgtt cgagttcttc gcttgcaagg cgttggcaca atgtaagccg aaaccaccga
     8341 caccgcagaa caagtcccac aatttagcaa taggtaaatg ctcaatccag ctttgtgctg
     8401 tgtggtaaag ctggcttgct accacagggt tagtttggaa aaagccctga gggcgaataa
     8461 atagcggtat gccgttaaaa tgttcttcca gcactttttg ttcggtgagg aaaatctcag
     8521 tttcgccttc taaaagagca gcgtgttggg gctgaatatt cagactaact actgagttgg
     8581 gtggcagttt ttccaacagt tcgggcaatt cccgttcaac caatgcacgc tttgcttcac
     8641 tacgaaccac aaaacggagc ataaatgatt gattatattg gctttcggta atcagaatat
     8701 attttagctc accttttttc ttactgatgt tatacggcac taaaccagct cgagcgataa
     8761 aatctttcaa aatcggaaaa agtctatgaa atgaggacgg gtagagcaga cagtcggtta
     8821 aatccacccc gctttgagca tcattttgat cttttaaaat gcctaaaatc gggcgttcta
     8881 cactgccgga gaccaccatt ttcgctttgt tacgaaattg cgaaattggc gagccgacaa
     8941 gcggtaagat ttccgtttta ttttgcaaaa taaacggcaa aatcaaccgc ttgagatcgg
     9001 cctctttggc ggctaattgc gtgctgtatg gcttttcaat ccactggcac gaagtacagt
     9061 cgccacgctc aaaatgcgaa caatgcaaca agctagacat tcgcttcctt ccattccagc
     9121 cagttgtttt gtaaggccgt taattcagcc tgagtttgtt ggtagcgaga gctgttttcc
     9181 agctctcgcc aagtcacggt tttgcgtttc attaaagaaa ggcgacgttc tttttgtgct
     9241 tgagtcggct gatatttcag cccgtctaac acttgcacca ggccttcagg ctcgttacaa
     9301 aggagcaata aatcacagcc ggcttggatt gctttttcag aacgctcgac aaaattgccc
     9361 ataaagcccg cacctttcat tcccaaatcg tcagagaata tcacaccgtt aaagttcagc
     9421 tgtttacgca atacctcttt aagccaatag cttgaaccgc ttgccggctg gctatcacat
     9481 tgggtgtaaa cgacgtgggc aggcataatg gcagagagtt ttcctttggc aataagctgt
     9541 ttgaacggca gaatatcgtg attaaaaatc cgctctttcg ggcgatcatc aaacggggtt
     9601 tctaaatgcg aatcggctaa aacgtgtcca tgaccgggga agtgtttgcc ggtggtcgcc
     9661 ataccgattt cacgcattcc gtcaataaag gcttcggcaa caggcaaaat cgctgctggc
     9721 tcttgcccga atgagcgatc gccaatcgct ttgcattcgt gtcctaaatc cagtaccgga
     9781 gcgaaactga ggtctatatc gagggcaaac atttctgctg ccatcaacca gccggtttct
     9841 ttcgctaatt gcattgcttg ctctggcgtt gtggcaagtt ggttaaaaga ttgcattgcc
     9901 ggcagtttag taaagccttc acgaaagcgt tgcacccgac cgccttcttg gtcgacagta
     9961 ataagcaacg gttttttcac ccgctggcga atggatttca ctaatgcctg aatttgtgct
    10021 ttgtcgtaga aattacgggt gaataaaatt agcccggaaa ctaaagggtg ttccagcatt
    10081 tcgacttctt cttgagtcag ttctttatct ttaatatcaa tcagtaacat aattttcgtt
    10141 gagctatttg caaaaaaatg agcagatatg accgcttgtt gtgcctattc tacattaaaa
    10201 agcccttgat gttgatcaag ggcgaatgat taagggcata aatgagacag agcaattatg
    10261 cttgttctaa tttagcttgg ttacgacgca tttttatgtt gagcatttca accacaaccg
    10321 agaagcccat tgccgtataa accgctgctt ttggaatatg aatatcaaaa ccttcgccga
    10381 ctagtacaat accgattaaa atcaagaacg ctaaagcgag atttttaatg gttgggttgt
    10441 tatctacaaa gtccccaatg gttttcgctg ctaacatcat taccgcaact gcaatgataa
    10501 tcgcaataac cattaccgga atatcatctg ccattgccac agcagtaatg actgaatcaa
    10561 gggagaaaat aatatcaaaa attgcaattt gaattaacac cataaaaaag ctggcttttt
    10621 taatggattc gcttttttct tcgtgagatt cggcagaaat ggattctttc agctccattg
    10681 cacttttaac aatcaggaat aatccaccga tgagtagaat gagatctcta cctgaaattg
    10741 gattgttgcc aatagtgaaa agaggctcga ccaaacgcat catccaagca agcgagagaa
    10801 gcaataaaat acgggttccc attgccagcc ccaagccgat aattcgggca gattggcgtt
    10861 gttcaatagg taaacgggaa acgagaatac tgatgacgat gatgttatca atgcctaata
    10921 cgatttcaag cccggtaagt gtaaagagcg caacccaagc ttcaggattt gtgatccagt
    10981 caaacataaa aatgtccttt taaaaataag ggattaagtg atattttcta tcaaatagaa
    11041 ttaaagccga catcttatag agtgtgagga caaatttaaa gggggcaatg caaaaagttt
    11101 tgtaaaattt cggcggtaaa atgtttacgc aaatagaaac caagagggag agattgaaca
    11161 atctcccctt tagcgttgat agcagaggtg atagagcggt tatttctacc tttgggtctg
    11221 agttgtaaca cttcgcctaa ggttgcatta attttatgca gttcacctaa cacaatcaac
    11281 tccattaatt cctcccaatc ttgctgtagg cgggattctt gtgtgatagt cggagaccat
    11341 aaaatcggct gaccgatatg gcgttcggca aggggtatct ttcgttcccc ttcaaccgga
    11401 atccaaagca cacgggaaag cttatgttta acgtgggagg tttcccaaat aataccgttg
    11461 ttttgggtta aaggagcaag gctgacgaat gtggtttcca gcggtaatcc tttctgattg
    11521 accggaatgg ttttcagctc aatgcctaaa tgtgcaaaat cctgttcggc tttactgccg
    11581 gctttcgcac ctaaagcggt ttcaatcaga gtaccgaccc agcctttatc tcgtttcaga
    11641 tctgctggta tcggaatatg aagcatctcg gcaatttcac ctaaagtgaa ccctgccagc
    11701 cattccgctt tgcttaataa tgcctgctcg gttggagaga atgtagataa ttgcattgca
    11761 cttagttaat tagactaaac tggcaagcgg tcaaatttag gcagaaattt gcaaaaaatt
    11821 actaaaaaat aaccgcttgt gaggaaatta catccaagca ttactgcgaa taacgcctac
    11881 tgcaatgcct tcaatttcaa tatattcttg agcagggttt actacaatcg gttttaattc
    11941 atcgttttca gggtgtaagt aaattaattc acctttcttc tctaagcgtt taacagtcac
    12001 ttcatcattt acccgagcaa ccaccacttg cccgttacga gcggtattgg ttttatgaac
    12061 ggctaatagg tcaccgtcta aaataccgat tttttccatt gaattgccat ttactttgag
    12121 taaataatca gcacttggat taaacatcgc accattcacc gggtaatggt tttcaacgtg
    12181 ttcaaccgct aaaataggtg tgccagcagc tactttgcca atcaacggta agccttcatc
    12241 ttcatcgttg gcagcttggt tttcgtcttc gactaaaatg cgaattccac gagatgtacc
    12301 tgataacatt tcaatatagc ctttacgagc aagggctttt aagtgttctt ctgccgcatt
    12361 aggtgattta aagccgattt cacgggctat ttctactcgg gttggtggca taccggtggt
    12421 ttcaatatgg tttttgacga aatcaaaaat ttcttgctga cgggcagtta gatgtttacg
    12481 cattttaagt taatcctcac tgtctttata tacagaaaat attgat
//
LOCUS       DANLXF010000060        12514 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.060, whole genome shotgun sequence.
ACCESSION   DANLXF010000060 DANLXF010000000
VERSION     DANLXF010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12514
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.060"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            145..1335
                     /locus_tag="PXD66_001070"
     CDS             145..1335
                     /locus_tag="PXD66_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HDL6319381.1"
                     /translation="MSTSQQLIQQTEHFGANNYLPLPIVISKAKGIWVEDPEGNRYLD
                     MLSAYSAVNQGHCHPKIVQALKDQADKVTLTSRAFYNDQLGIWYEKICKLANKEMALP
                     MNTGAEAVETAIKTARRWGYDVKGIAENQAEIIACVGNFHGRTMAAVSLSSDPDYKRG
                     FGPMLGGIKLVPYGDLEALKAAITPNTAAFLVEPIQGESGILIPPEGFLEQAYELCKE
                     NNVLFIADEIQAGLGRTGKLFATEWEGFEPDIYILGKALGGGVFPISCVLANRDILGV
                     FNPGSHGSTFGGNPLACAVSIASIDVLLEEKLAERSRELGEYFLAELKKIQNPIIKEV
                     RGRGLFIGLELHQPARPFCEKLKEKGLLCKETHDTVIRFAPPLVIEKFNLDWALEKIR
                     EVLA"
     gene            1410..2921
                     /gene="nhaC"
                     /locus_tag="PXD66_001071"
     CDS             1410..2921
                     /gene="nhaC"
                     /locus_tag="PXD66_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL6319382.1"
                     /translation="MLILQTFSKNQPLVKGGFMSNRTEITDQNIRIPFRYAILPVIIM
                     VSIMAVAIIKFEASPHVPLLIGAISAAVIAMAHGYRWETIEKGIYRGIRMALPAIVII
                     IMIGLTIGAWLGGGIVATMIYYGLKLISPSLFLVTISIICAIVTLAIGSSWSTMGTIG
                     VAAMGIGFSLGIPAPMVAGAVISGAYFGDKMSPLSDTTNLAAGITETNLFEHIRHMFI
                     TTVPAFLIALSVYFYFGMEFRAENLDLAKIEDIMQAMQEHFLISPWLLLVPLLVVVLV
                     AKKVPALPALAAGIILGWLCHVFVQGGDVSVAVITLHDGYKIESGHEMIDKLFNRGGI
                     ESMMYTISLTIVAMTFGGIAEQAGMLHSVVEKILTLAKSAGSLISATIVSAFITNVTT
                     SEQYISILLPGRMYIKAYRDKGLSSRNLSRALEDGGTVTSVLVPWNTCGVYAFSMLQV
                     SAFEYAPYAIMNWLIPLIAIGLATLNIKIERLPPEELAKEPEEITLPKRGCCP"
     gene            3038..6637
                     /gene="putA"
                     /locus_tag="PXD66_001072"
     CDS             3038..6637
                     /gene="putA"
                     /locus_tag="PXD66_001072"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HDL6319383.1"
                     /translation="MMSYQLLPNTTIRPELSRHYRFDEQTLVQNLIEQAHCDEQADNI
                     KKLTTQLVGKVRTARQKASGVDALMHEFSLSSQEGVALMCLAEALLRIPDTATADKLI
                     RDKIAKGDWRSHTGNSPSLFVNAAAWGLVITGKLVSTHSESSLSSALSRLIAKGGEPL
                     IRKGVDVAMRMLGKKFVTGETIQQAIKNGEKRFAMGYRYSYDMLGEAALTAEDAKRYY
                     NDYVASIHAVGATSKGLGVYKSSGVSVKLSAIHPRYSLSQHQRVMDELYPRLKELFLL
                     AKKYDIGLNIDAEEADRLELSLDLMDRLLADPDLAGFNGIGFVVQAYQKRCPYVIEHL
                     IETARKHNRQLMIRLVKGAYWDTEIKRAQMDGAEGYPVFSRKVHTDLNYIVCAKKLLA
                     AQDVIYPQFATHNAQTLATIYHLAQGKRFEFQCLHGMGESLYDQVVGSDNLNVQCRIY
                     APVGTYQTLLAYLVRRLLENGANSSFVSQIVDENLSVDDLAQAPVQKAELTNGTMHPK
                     IPLPVDLFDDNRVNSKGYDLTDAVSLQNLENALNELTKQHYRAEPQLANDNTSGQESR
                     KIYNPANQAELVGEVIDATENDMEQAFESAAAFQSEWANTSPAVRAECLEKMADLMEA
                     NMPQLFDLAVREAGKTLNNAIAEVREAVDFCRYYAKEVRNNIEAYKNPRGIVVAISPW
                     NFPLAIFVGEVSSALVAGNVVLAKPAEQTSLMAHYAVSLFYQAGVPKAALQCLPGRGK
                     TVGVALVADHRVNGVIFTGSTETAKNINRNLHKNEKIIPLIAETGGQNAMIVDSSALG
                     EQVVIDVLNSAFDSAGQRCSALRVLYLQRDVADHILTMLKGAMAELKVGLPQHLTTDV
                     GPVIDKVAQQRLLDHIENLKKSAKSYYQVAISSEIAENGIFVPPTLLEIDHISQLKHE
                     VFGPVLHVIRFDADKLDQVIADINSTGFGLTSGLHSRIDETVDLWLKSVHAGNLYVNR
                     NTVGAVVGVQPFGGMGLSGTGPKAGGPLYLQRLVNRQEWTLNGLQGNAQAVDFDLNFA
                     IRKVLSGAEQQTALDLAKSLARRSPLGKTYPMQGITGEKNEMIFVARPQIGISKGNLA
                     SQVQALIAIVTVGSQAVAEKSSELAKHASAFGKKLQVVDDFNSLDHLSTLIVLDLLSS
                     EEKLHHAERKGAILTYIEGLDLALSLLPLVHEKAISINTAAAGGNASLMSEMD"
     gene            6746..7396
                     /locus_tag="PXD66_001073"
     CDS             6746..7396
                     /locus_tag="PXD66_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595-like element ISMha3 family transposase"
                     /protein_id="HDL6319384.1"
                     /translation="MRKSRLSQYKQHKLIELFVAGVIARTAAELVNVNKTTAAYYFHR
                     LRLLIYQHSPHLEMFEGEIEIDESYFGGHRKGKRGRGAAGKTAVFGLLKRDGKVYTVV
                     VPNTQSATLLPIIREKVKPDSLVYTDFYRSYDVLDVSEFNHFRINHSTHFAENQNHIN
                     GIENFWSQAKRHLRKFNGIPKAHFELYLKECEWRFNHSDLKSQIFLLKQLVKGGLG"
     gene            complement(7397..8284)
                     /locus_tag="PXD66_001074"
     CDS             complement(7397..8284)
                     /locus_tag="PXD66_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide transport protein"
                     /protein_id="HDL6319385.1"
                     /translation="MNKLTQLALISLAFTGSAFAAPKSFTYCLESSPAALNPQIATDG
                     ATLDVGQAIYNRLIDFEKGTTNLTPSLAERWEISEGGKIYTFFLRQNVQFQSNKSFTP
                     SRYFNADDVIFSINRQFDKTNPYHKLSGGNYEFFYGMNMQNIIKNVTKVDDYTVKIEL
                     SEPNAPFLANLAMDFMAIFSAEYANKMQIAGTPEKLDLEPIGTGAFQFYGYQKDAAVR
                     FTAFEHYWEGKAKIDRLVFSITPDASVRLAKLEKNECQAMPYPNPSDLSALKANKDIQ
                     LFEKSGLNIGYITFRGCPR"
     misc_feature    complement(8310..8455)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            complement(8632..9642)
                     /gene="mglC"
                     /locus_tag="PXD66_001075"
     CDS             complement(8632..9642)
                     /gene="mglC"
                     /locus_tag="PXD66_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018355241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HDL6319386.1"
                     /translation="MSALQQNRTLDFFKQNAIYFVLLILLIVIIVQDPSFLSLRNFSN
                     ILSQSSVRLIIALGVAGLIVTQGTDLSAGRQVGLAAVISATLLQSMENMNKVFPNLSE
                     IPIPVVVLTVCAIGAVIGLFNGFVVAYLNVTPFIATMGTMIIVYGINSLYYDAVGSSP
                     IAGFSDDFSTFAQGFFRFGDFRLSYITIYAVIATIIMWTLWNKTRFGKNVFAIGGNPE
                     AARVSGVNVPLNLMGIYMLSGIFYAFGGMLEAGRIGSATNNLGFMYEMDAIAACVVGG
                     VSFAGGVGTIIGVVTGVLIFTVINYGLTYIGVNPYWQYIIKGGIIILAVAIDSLKYAK
                     KK"
     gene            complement(9656..11155)
                     /gene="mglA"
                     /locus_tag="PXD66_001076"
     CDS             complement(9656..11155)
                     /gene="mglA"
                     /locus_tag="PXD66_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005709009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HDL6319387.1"
                     /translation="MTGEVLLTMTNVSKSFPGVKALDNANLTVKSHSVHALMGENGAG
                     KSTLLKCLFGIYAKDEGDILFLGKPVNFKTSKEALENGISMVHQELNLVKQVTVMDNL
                     WLGRYPLKGPFVDHDKMYRDTKAIFDELDIDVDPRDKVAKLSVSQMQMIEIAKAFSYN
                     AKIVIMDEPTSSLSEKEVEHLFKIIQKLKDRGCGIIYISHKMDEIFKICDEITILRDG
                     KWVNTVAIKDTSMDQVVSMMVGRELTQRFPEKTNLPKEVILEVEHLTAKNQPSIQDVS
                     FSLRRGEILGIAGLVGAKRTDIVETIFGVREKAGGTIRLNDKEINNRNAFDAINNGFA
                     LVTEERRSTGIYANLSIEFNSLISNMKSYLGKFGLLSNKKMKSDTQWVIDSMNVKTPS
                     HQTHIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDIGAKFEIYQLILELANKDK
                     GIIMISSEMPELLGVTDRILVMSNGKVAGIVETAHTSQEEILQLAAKYL"
     gene            complement(11227..12216)
                     /gene="mglB"
                     /locus_tag="PXD66_001077"
     CDS             complement(11227..12216)
                     /gene="mglB"
                     /locus_tag="PXD66_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015701875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HDL6319388.1"
                     /translation="MKKAVLSAVAMAVALGATISTAQAENRIGVTIYKYDDNFMALMR
                     KEIEKEGKTQKVELLMNDSQNTQSIQNDQVDVLLSKGVKALAINLVDPSAYKTIIQKA
                     KSEDVPVVFFNKDPGAAAIDSYPKAYYVGTEPKESGIIQGELIAKQWKAHPELDLNKD
                     GKIQFALLKGEPGHPDAEARTKYVVESLKAQGIDSEQIFMDAALWDAAQAKDKVDAWL
                     SSGKAKDIEVIISNNDGMALGALEATKAHGRKLPIFGVDALPEALQLIKKGELAGTVL
                     NDGVNQGKAVVQLSANLADGKDAVAGTQWKLENRVVRIPYVGVDKDNLSEFLK"
BASE COUNT         3589 a         2642 c         2675 g         3608 t
ORIGIN      
        1 atctattcat attaattgca tagttaatca acaactgaag aaaaattaga agtagatcat
       61 aaaatcaaca aaaagtccgt gctttttagc taaagattgt taaactcttt tgtgttacaa
      121 gtttttaaca taaaggagtt ttttatgtct acttctcaac aactcatcca acaaaccgaa
      181 catttcggtg ccaataacta cctacctcta cctattgtta ttagtaaagc taaaggcatt
      241 tgggttgaag atcctgaggg taatcgctat ttggatatgc ttagtgccta ctcagcggtt
      301 aatcaagggc attgtcatcc aaaaattgta caggcactaa aagatcaagc agacaaagta
      361 acgctgacct ctcgagcatt ttataacgat caacttggta tttggtatga aaaaatctgc
      421 aaactcgcta ataaagagat ggcattaccg atgaatacgg gtgcagaagc ggtagaaaca
      481 gcgattaaaa ctgctcgccg ttggggttat gatgtgaaag gaattgcaga aaatcaagca
      541 gaaatcatcg cttgtgttgg caattttcac ggacgaacaa tggctgcagt atctctctcg
      601 tctgatccgg attataaacg tggtttcggg ccgatgttag gtggcatcaa acttgttcca
      661 tacggtgatt tagaagcatt aaaagctgcg attacaccaa acacagcagc gtttttggtt
      721 gagccaatcc aaggtgaatc tggcattctt attccacctg aaggtttctt ggaacaggct
      781 tatgaactat gcaaagagaa taatgtgctg tttattgctg atgaaattca ggcaggtctt
      841 gggcgtactg gcaagttatt tgcaacagaa tgggaaggct ttgagccaga tatttatatt
      901 ctaggtaaag cacttggtgg cggggtgttc ccaatctctt gtgtgttagc aaatcgtgat
      961 attttaggcg tattcaatcc tggctcgcac ggttcgacat ttggtggtaa tccattagcc
     1021 tgtgcggtat ctattgcctc aattgatgta ctgttggaag aaaaacttgc agagcgttcc
     1081 cgtgagttag gtgaatactt cttggcagag ctgaaaaaaa tccaaaatcc aattattaaa
     1141 gaagttcgtg gacgtggact gtttattggc ttggagttac atcaaccagc acgcccattc
     1201 tgcgaaaaac tcaaagaaaa aggcttactc tgcaaagaga cccacgatac agttatccgc
     1261 tttgctcccc ctttggtgat tgaaaaattc aatttggatt gggcattgga gaaaattcgg
     1321 gaggtgttgg cttaattgtc attttctcta tattttatcc tgccaaatcg cctttaaccc
     1381 tttttggcag gaagcaaaac agttgtaaaa tgctgatttt gcaaactttt tccaaaaatc
     1441 aaccgcttgt aaaaggtggc tttatgtcaa acagaacaga aataacagac caaaatatcc
     1501 gaatcccgtt tcgctatgct atattgcctg tgattattat ggtatcgatt atggcggtgg
     1561 ctatcatcaa atttgaagcc tcacctcacg ttccgttgct tattggggca attagtgccg
     1621 ctgtgattgc aatggcacat ggctaccgtt gggagacaat tgaaaaagga atttatagag
     1681 gcattcgtat ggctttacca gcgattgtta tcattattat gattgggcta accatcggag
     1741 cttggctagg tggtggaatt gtagctacga tgatttacta cggcttgaaa ttgatctctc
     1801 cgtcgctgtt tttagtaact atctcaataa tttgtgctat tgtgaccctt gcgattggta
     1861 gctcgtggtc tacaatggga acaatcggtg tagcggcgat ggggatcggg tttagtctcg
     1921 gcattccggc accgatggtt gccggtgcgg tgatctcagg agcatatttc ggcgataaaa
     1981 tgtcgccact gtcagacact accaatctgg cagcagggat taccgaaacc aatctgtttg
     2041 aacatatccg ccatatgttt atcactaccg tgccggcgtt cctgattgcg ttatctgtct
     2101 atttctattt tggtatggaa tttagagccg aaaatctcga tcttgctaaa attgaagaca
     2161 ttatgcaggc aatgcaggaa catttcttga tttcgccttg gttgctatta gtgcctttat
     2221 tagtcgtggt gttggtggca aaaaaagtgc ctgccttacc ggcattagct gcagggataa
     2281 ttcttggttg gctctgccac gtttttgtgc aaggcggtga tgtcagcgta gcagtgatta
     2341 ctctgcacga cggctataaa attgagagcg gccacgagat gattgataag ctgttcaacc
     2401 gtggtgggat cgagtcgatg atgtacacca tttcccttac gattgttgcg atgacttttg
     2461 gtggcattgc agagcaagct ggaatgttac attcagtagt ggaaaaaatc cttacccttg
     2521 caaaatcagc aggtagctta attagtgcta cgattgtatc ggcatttatt accaacgtaa
     2581 cgacatctga gcaatatatt tctattttat taccaggcag aatgtatata aaagcctata
     2641 gggataaggg actaagttcg agaaaccttt ctcgtgcatt ggaagacgga ggaacagtta
     2701 cttcggttct tgtaccttgg aatacctgtg gtgtttatgc gttctcaatg ttgcaagtga
     2761 gtgcttttga atatgcaccg tatgcaataa tgaattggct cattccactg attgcgattg
     2821 gcttggcaac cttgaatatc aaaattgagc gattgccacc ggaagaatta gccaaagaac
     2881 ctgaggaaat tactctgcca aaacgtggtt gttgtccata atttatctaa atagtattta
     2941 ataacaatag gttaagttaa ataacaagcg gtcggtttta tcttaaaatt tgcaagttta
     3001 tacataaatt tgatcgcttg ctgtatagaa aggaaaaatg atgtcatacc aacttttacc
     3061 taacaccact atccgtcctg aattgagccg tcattaccgc tttgatgagc aaaccctggt
     3121 gcaaaatctg atagaacaag cacattgtga tgaacaggct gataacatta aaaagctcac
     3181 tacccaatta gtcggtaaag tccgaactgc acgccagaaa gcaagcgggg ttgatgcgtt
     3241 aatgcacgaa ttttcactct ccagccaaga gggggtggcg ttgatgtgcc ttgccgaagc
     3301 attactacgc attcctgaca ccgccactgc ggataaatta attcgagaca aaattgccaa
     3361 aggggattgg cgttcacaca ccggcaacag cccctctctg tttgtgaatg cagcggcgtg
     3421 gggcttggtg attaccggca aattggtttc aacccacagc gaaagctcac tctcatcagc
     3481 actttcacgc ctgatcgcca aaggcggaga accgctgatc cgcaaaggtg tggacgtagc
     3541 aatgcgtatg ctcggcaaga aatttgtaac gggcgaaacc attcaacagg cgattaaaaa
     3601 cggcgaaaaa cgcttcgcaa tgggttatcg ttacagctac gatatgctgg gcgaagcggc
     3661 actcaccgcc gaagatgcca aacgctatta caacgactat gttgcctcta ttcacgctgt
     3721 gggtgccacc tcgaaaggct tgggcgtgta taaatcctcg ggtgtgtcgg tcaaactctc
     3781 cgccattcac cctcgttatt ccctttccca acaccaacgt gtgatggacg aactctatcc
     3841 acgtctgaaa gaactgttct tgctggcgaa aaagtatgac atcggcttga acattgatgc
     3901 cgaagaagcc gaccgcctcg aattgtcgct tgatttgatg gacagactgc ttgccgaccc
     3961 tgatttggca gggtttaacg gcattggttt tgtggtgcag gcgtaccaaa aacgttgccc
     4021 gtatgtgatc gagcatttaa ttgagaccgc ccgtaaacat aatcgccagc taatgattcg
     4081 cttggtaaaa ggcgcatatt gggacactga aatcaagcgg gcgcaaatgg acggtgcaga
     4141 gggctatccg gtcttctccc gtaaggtgca taccgatttg aactacatcg tttgtgccaa
     4201 aaaattgctg gcggcgcaag atgtgatcta tccgcaattt gccacccaca acgctcaaac
     4261 tctcgccacc atttaccact tggcacaggg caaacgtttt gagtttcaat gcttgcacgg
     4321 tatgggagag agcttgtacg accaagtggt tggcagcgat aatcttaacg tgcaatgtcg
     4381 catttatgcc cctgtcggta cttatcaaac cctgcttgcc tatttagtac gtcgcttgtt
     4441 ggaaaacgga gcgaacagct catttgttag ccaaattgtg gatgaaaatc tcagtgtaga
     4501 tgatctggct caagcccctg ttcaaaaagc agaactgacc aatggcacta tgcacccgaa
     4561 aattccattg ccagtcgatt tatttgacga caatcgtgtt aattccaaag gctatgattt
     4621 aactgatgca gtaagcctac aaaatttaga aaatgccctc aatgaactta ccaaacagca
     4681 ttaccgtgct gaaccacagc tggcaaatga taatacaagc ggtcaagaaa gccgaaaaat
     4741 ttacaatcca gcgaaccaag ctgaattagt cggcgaagtg attgatgcca ccgaaaacga
     4801 tatggaacag gcgtttgaaa gtgcggcggc gttccaaagt gaatgggcga atacttcccc
     4861 tgcggtgcgt gccgagtgct tagaaaaaat ggcggacttg atggaagcca atatgccaca
     4921 actgttcgat ttggcggtgc gtgaggcagg caaaacgctc aacaatgcaa ttgctgaagt
     4981 gcgtgaagcc gtcgatttct gtcgttatta tgccaaagag gtacgcaaca acattgaagc
     5041 atacaaaaat ccgcgtggca ttgtagtagc aattagcccg tggaactttc cgttagcgat
     5101 ttttgtgggt gaagtttcat ctgcgttggt ggcaggcaat gtggtactag caaaaccagc
     5161 tgaacaaact tcgttaatgg cacattatgc tgtcagcctt ttctaccaag caggtgtgcc
     5221 gaaagcggcg ttgcaatgct taccgggtag aggaaaaacg gttggggtgg cgttggtggc
     5281 agatcatcgt gtgaacggcg tgatttttac tggttcaaca gagacagcta aaaacattaa
     5341 ccgcaatttg cacaaaaacg agaaaattat accgcttatt gccgaaactg gcggacaaaa
     5401 tgcaatgatt gttgatagct cggcactggg cgagcaggtt gtgatagatg ttttaaactc
     5461 cgcatttgac tcagcagggc aacgttgttc ggcattgcgt gtgctgtatc tgcaacgtga
     5521 tgttgccgat cacattctca caatgctcaa aggagcaatg gcggagttga aagttggtct
     5581 tccacaacac ttaactaccg atgtcggacc agtaatcgat aaagtcgcac aacaacgctt
     5641 gctcgatcat attgaaaatc tcaaaaaatc ggcgaaaagt tactatcagg tggctatctc
     5701 ctctgaaatt gccgaaaatg gtattttcgt accgccaacg ctgttagaaa tagatcatat
     5761 cagccagctt aaacacgaag tattcggacc ggtgctacac gttatccgtt ttgatgcgga
     5821 taaactcgac caagtgattg ccgacataaa ctccacaggt ttcggtctaa ccagcggttt
     5881 gcatagccga attgatgaaa cggtggattt atggttgaaa tctgtccacg ctggaaatct
     5941 gtatgtaaac cgtaacacgg taggggctgt ggtcggcgta cagccattcg gtggaatggg
     6001 gttatcagga actgggccga aagctggcgg gccgctctat ctacaacgtt tagtaaaccg
     6061 ccaagaatgg acattgaacg gtttacaggg caatgcacaa gcggtcgatt ttgacttaaa
     6121 ttttgcaatc cgcaaggttc taagtggagc tgagcagcaa acggcgttag atctggccaa
     6181 aagccttgca cgccgctcgc cactcggaaa aacctaccca atgcagggta ttaccggcga
     6241 gaaaaacgag atgattttcg tggctcgccc acagattggg attagcaaag gtaatttggc
     6301 ttcacaggtg caagccttga ttgccattgt aacggtcggc agccaagcgg tggcagaaaa
     6361 atcaagcgag ctggcaaaac acgcctctgc ctttggtaag aaattacagg tggtggatga
     6421 tttcaacagt ctcgatcacc tcagcacact cattgtgctt gacctgcttt ctagtgagga
     6481 aaaattgcat catgccgaac gcaaaggggc gattttgacc tacatcgaag ggctggattt
     6541 agcgctctca ctgctgccgt tggtacacga gaaagcaatc agcatcaaca ccgctgccgc
     6601 agggggaaat gccagcttaa tgagtgaaat ggattaagca acaaacaaaa gagtataaga
     6661 gggcagatca caatgatctg cctttttatt ttaaggggct gtcctagata acgactaaaa
     6721 cttccattta ggttaagata accctatgag aaaaagtcgt ctaagtcagt acaaacaaca
     6781 taaactcata gagctgtttg tagcaggcgt aatcgctcga acagccgcag agttagtgaa
     6841 tgtaaataaa accaccgcag cctattattt tcaccgtttg cgattgctca tctatcaaca
     6901 tagccctcat ttggaaatgt ttgagggaga aattgagatt gatgagagct atttcggcgg
     6961 tcatcgcaag ggtaaacgag gtcgtggtgc agcaggcaaa accgccgtat tcgggctttt
     7021 aaagcgtgac ggcaaggttt ataccgtagt agtgccgaac acacaatctg ctacactttt
     7081 gccgattatt cgggaaaagg tgaagcccga tagccttgtt tacaccgatt tttatcgaag
     7141 ctatgatgtg cttgatgtga gtgaatttaa ccattttcgt atcaatcaca gcacacactt
     7201 cgcagaaaat caaaatcaca ttaacggaat tgagaatttt tggagtcaag caaaacgtca
     7261 tttacgcaag tttaatggta ttccgaaagc ccattttgag ctatatttga aagaatgcga
     7321 atggcgtttt aatcacagtg acttaaaatc tcaaatattc cttttaaaac aattggttaa
     7381 aggggggtta ggttagttat ctaggacagc ccctaaaagt aatataaccg atatttaggc
     7441 ctgacttttc aaataattga atatctttat tcgcttttaa agcagacaaa tctgacgggt
     7501 tcggataagg catcgcttgg cattcgttct tctccaattt agctaaacgt actgaggcat
     7561 cgggcgtaat actaaataca aggcgatcta tcttcgcttt tccttcccaa tagtgttcaa
     7621 atgctgtaaa tcttaccgct gcgtcctttt ggtaaccata gaactggaat gcacctgtgc
     7681 ctataggttc taaatctaat ttttccggcg tacccgctat ttgcatttta tttgcatatt
     7741 ccgcagaaaa gatcgccata aaatccatag ctaaattagc aagaaagggg gcattcggtt
     7801 cagataattc gatttttacc gtgtaatcat ctacttttgt tacatttttg atgatgtttt
     7861 gcatattcat tccgtagaaa aactcatagt taccgccgga caatttgtga tatggattcg
     7921 tcttatcaaa ctgacggtta atcgaaaaaa taacatcatc agcattaaaa taacgactag
     7981 gggtaaaact tttattactc tggaactgca cattctgacg caagaaaaaa gtataaattt
     8041 ttccgccttc actgatttcc catcgctctg ccagagaagg agtgaggtta gtggtacctt
     8101 tttcaaaatc aatcaggcga ttatagattg cttgcccgac atcaagagta gctccatctg
     8161 ttgcaatctg aggattcaat gctgccggag atgattccaa acagtaagta aaagatttag
     8221 gtgctgcgaa agcagaacct gtaaatgcta aagaaataag tgcaagttgt gtcagcttgt
     8281 tcattgtcat atccttaaat gttaagcaat aaaaaaccct ttcaaaagtt gccttccgaa
     8341 agggttagaa ttgggtttgc gattttggat tgcgtttgcc atttcaggtt ttcggaagat
     8401 atcttccgaa cacgccaaat gcccgaaaga ttaatcggtc aaatggtagt gatgatgttt
     8461 aacgcaatag ctcataatat tctccaaaat tgaatgttta ggaatttacg ctttttaaaa
     8521 atcaattgca aacgttttct ttaaaatttt gtaaaaaatt caacattttt caccgcttgt
     8581 atcgtaccta ggagcaaatg gcgatttgct cctaggtttc caagcaatag gctatttttt
     8641 cttcgcatat ttaagtgagt caatcgctac cgccaagata atgatgccac ctttaataat
     8701 atattgccag taagggttta ctccaatata ggttaagccg tagttgataa cggtgaagat
     8761 taacacccct gttactacac cgatgatagt acccacacca ccggcaaagg ataccccacc
     8821 taccacacaa gcggcaatgg catccatttc atacataaag cctaagttgt tggttgcact
     8881 accgatacga cctgcttcta acataccgcc gaaggcatag aagatgcctg aaagcatata
     8941 aatacccatc aggttaagcg gtacgtttac accggaaact cgtgctgctt cagggttgcc
     9001 gccaatagca aatacgtttt taccgaagcg ggttttattc cataacgtcc acataataat
     9061 ggtagcaatc acggcataaa tagtgatgta ggatagccta aagtcgccaa agcggaagaa
     9121 gccttgtgca aaggttgaga aatcatcgct aaagcctgca attggcgatg agcctactgc
     9181 atcgtagtaa agcgagttga taccatacac aataatcatt gttcccatcg tggcaataaa
     9241 cggtgttacg ttgaggtaag ccaccacaaa gccgttaaat aaaccaatga cagcaccgat
     9301 ggcacacacg gttaatacaa ccacaggaat cgggatttcc gagaggtttg ggaatacctt
     9361 gttcatattt tccattgatt ggagcaaggt ggcggaaatt accgctgcca agcccacttg
     9421 gcgaccggca gataagtctg tcccttgtgt tacaatcaag cctgcaaccc ctaatgcaat
     9481 aatcaaacgc acagaagatt gcgataaaat gttactgaag ttacgcaagc tcaagaaact
     9541 tgggtcttgc acaataatca caataagtaa gattaataat acgaagtaaa tcgcattttg
     9601 tttaaaaaaa tcgagtgttc tattttgttg taaagcagac atacttcact ccttgttata
     9661 aatattttgc tgcgagttgc aagatttctt cttgcgaggt gtgagcggtt tcgacaatac
     9721 ctgcaacttt cccgttactc atcactaaaa ttcggtctgt gaccccaagc aattccggca
     9781 tttccgatga aatcataatg atgcctttat ctttattcgc cagctctaaa atcaactggt
     9841 aaatttcaaa ttttgccccg atatcgatac cacgggtcgg ctcgtcaagc atcaagattt
     9901 caggctgggt taacaaccaa cgcccgataa tcactttttg ttggttaccg ccggaaagtg
     9961 agccaatatg cgtttgatgc gatggggttt ttacattcat cgaatcaatt acccattggg
    10021 tatcgctttt cattttttta ttgctcagta agccgaactt gcctagataa gacttcatat
    10081 ttgaaatcag agagttaaac tcaatgctta agttggcata aatccctgtt gaacggcgtt
    10141 cttctgtaac caatgcaaag ccgttattaa tcgcatcaaa agcgttgcgg ttattgattt
    10201 ctttatcgtt tagtcggata gtgccgcctg ctttctcccg tacaccgaaa atggtttcaa
    10261 caatatcagt ccgttttgct ccaactaagc cggcgatgcc taaaatttcc cctctgcgta
    10321 agctaaagct gacatcttga atagagggtt gattttttgc cgttaaatgc tcaacttcaa
    10381 gaatcacttc tttcggcaga ttggtttttt cagggaaacg ctgggtcagc tcacgcccta
    10441 ccatcattga aaccacttga tccattgagg tatctttaat tgcaacggtg tttacccatt
    10501 taccgtcacg caaaatggta atttcatcac aaattttaaa aatttcgtcc attttgtgtg
    10561 aaatatagat aatgccacag cctctgtctt tcagtttttg gataatttta aataaatgct
    10621 cgacttcttt ttctgaaagt gacgaagttg gttcgtccat aatcacaatt ttggcattgt
    10681 aagagaaagc ttttgcaatc tcgatcatct gcatttgtga aaccgataat ttggctactt
    10741 tatcacgtgg atcaacatca atatctaatt catcaaaaat cgccttagta tcacgataca
    10801 ttttatcgtg atctacaaaa ggccctttta gtgggtaacg acctagccat aagttatcca
    10861 ttaccgtcac ttgttttact aggttcaact cttggtgaac cattgagatc ccattttcta
    10921 acgcctcttt tgaggtctta aaattcaccg gcttgccaag aaagagaata tctccctcat
    10981 ctttagcata aatcccaaat aagcatttca gtaacgttga tttacctgct ccattctctc
    11041 ccattaaggc gtgaacacta tgggatttca ccgttaagtt agcattatct aaggctttta
    11101 ccccgggaaa tgacttgcta acatttgtca ttgtgagtaa aacttcacct gtcatatctc
    11161 acccctgatt acatcgaaaa atcgaaaaaa agcacgggta tccatcaccc gtgctaaaag
    11221 cgtgaattat tttaaaaatt cagataagtt gtctttatcg acaccaacgt aaggaatacg
    11281 caccacacgg ttttccaatt tccattgtgt accagcaact gcatctttac cgtctgctaa
    11341 gttagcagaa agctgaacaa ccgctttacc ttggtttaca ccatcattta ataccgtacc
    11401 ggctaattca ccttttttga ttaattgaag tgcttctggc aatgcatcta caccgaagat
    11461 tggtaatttt ctaccgtgag ctttggttgc ttctaatgca cctaacgcca taccatcatt
    11521 attagaaata attacttcga tgtctttcgc tttaccactt gataaccaag catccacttt
    11581 atctttagct tgggctgcat cccataatgc tgcgtccata aagatttgct cactgtcgat
    11641 accttgtgct tttaagcttt ctaccacata tttagtacgt gcttccgcat cagggtgacc
    11701 cggctcacct tttaataagg caaattggat tttaccgtct ttattcaagt ctaattcagg
    11761 atgggctttc cactgttttg caattaactc accttgaata atgcctgact ctttcggctc
    11821 agtacctaca taataagctt tcggatagct atcaatcgca gctgcacccg ggtctttatt
    11881 gaagaacacc acaggcacat cttcactttt tgctttttga ataattgttt tataagctga
    11941 tggatccact aagttaattg ctaatgcttt aaccccttta gaaagtaata catcaacttg
    12001 gtcgttttga atagattgcg tattttgcga gtcattcatt aataactcga ctttttgtgt
    12061 tttaccctct ttttcgattt ctttacgcat taatgccata aagttatcgt catatttata
    12121 aattgttaca ccaatacggt tttcagcttg agctgtactg atcgttgcac ctaatgcgac
    12181 tgccattgcc actgcactta atactgcttt cttcatagta gatcacctct tttatgtgtt
    12241 tatagggaaa tgaattgctt accataagca acttggtgca aagtataact aattttaaaa
    12301 aaacaatccg tgatcattct cacagaatta agacaaagtg gagaaatttt taattttaag
    12361 tttgacatcc tcccctgcct aaaggcaggg gattcctact agctcccaca gcaagctgtg
    12421 ggctactctc ggtgggtttc tactgctgat ctctattgag attcacttca tagacgcttc
    12481 gggtatgtcc taccctgata tttattatgc ttgg
//
LOCUS       DANLXF010000061        12479 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.061, whole genome shotgun sequence.
ACCESSION   DANLXF010000061 DANLXF010000000
VERSION     DANLXF010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12479
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.061"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            50..268
                     /gene="cspD"
                     /locus_tag="PXD66_001078"
     CDS             50..268
                     /gene="cspD"
                     /locus_tag="PXD66_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein CspD"
                     /protein_id="HDL6319389.1"
                     /translation="MDIGIVKWFNNVKGFGFITSDVCEGDIFAHFSEIQAEGYRSLKV
                     GQKVQFELVQGERGASASNITPISEKTL"
     gene            358..1272
                     /locus_tag="PXD66_001079"
     CDS             358..1272
                     /locus_tag="PXD66_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL6319390.1"
                     /translation="MPSSYQLVLINLLYIFGIGLNIVLLRFLSLHFDPLNNNGIRFLV
                     GGFTLLFFILWRFRTSLSQLIKTPKLIAISLFVGLMMSANMYFWLKGVKITNAVTVSI
                     FGVLAMPFGVIIAAIFFQDERERIKSKAFWFGSILTIIGSLGFIWHGKTLSIGEGFIL
                     GSVFLFLSIIIRNIQNLVVKFARELNVLTLSCVTSLTTSLSSLLLSQQAGKFNELFTA
                     PNWLLFSLMVAGIYAIMIGMVLTFHIIQTQGLITYQVLELLMPVSTAIIAYILLDERI
                     SIVQMGFACIVILGSSIALKLVKYPKHY"
     gene            1391..2251
                     /gene="araC"
                     /locus_tag="PXD66_001080"
     CDS             1391..2251
                     /gene="araC"
                     /locus_tag="PXD66_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HDL6319391.1"
                     /translation="MYKNLSLLSPTYPFDLEFIAGLTQCEKGNYLDSIVDRQQGMHGY
                     MLQLTTFGRGSVFDGKHVFTAHRGQLLLFTPNAVQHYHRHPESQYWHYKWVYFQPNPK
                     WNKWLNWSDMEEKIGRITISDDRYFQEISQLFSKIELELKSSKFFKEDIATSLLEYLL
                     MKCISAEKIEVIPVIDQRILTVCDLILTNLAKNESLEAMAEKVFLSPSRLSHLFKQSL
                     GISLVQWRELQRISESKKLLYFSNISISNIAKSLGYEDSLYFSKIFKKHTGLSPSEFR
                     TLERRKDSEM"
     gene            2438..3412
                     /locus_tag="PXD66_001081"
     CDS             2438..3412
                     /locus_tag="PXD66_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL6319392.1"
                     /translation="MKKPTFKHALFSSILALACYGSAFANDVVNISKIDGMPWFNRMA
                     EGVVQAGKDNNINAYQVGPSNTDAPQQVKLIEDLIAKKVSAISIVPNDANALEPVLKK
                     AKQNGIVVLTNESVGQPSADWDIEIIDNAQFAADYVEEVAKAMGGKGGYVIYVGGLTV
                     PQHNLWADLFVKYQKEHYPDMFEVTSRMPVAENINDARRTTIDLVKAHSDLKAVVSFG
                     SQGPIGAGLAVKEKRLKNKLNVFGMMIPSQAANLIKSGDITMGITYDPANAGYALAAV
                     AAKVLKGEKIEDGLEIPTVGKAKVDSEKKLLQFHNVLKVTKENIDKLY"
     gene            3467..4957
                     /locus_tag="PXD66_001082"
     CDS             3467..4957
                     /locus_tag="PXD66_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL6319393.1"
                     /translation="MAFISMRHISKTFHGIKALNQVNLSLDYGEALCLAGQNGCGKST
                     LIKILSGVYQPDKGAEIQIGATQYTKLTPQESIDKGIQVIYQDLALFPNLTVAENIAI
                     NLHRKFGWVSQDHIHQVALTAIQSINANLDLNAILEDLPIAQQQLVAICRALAQNARL
                     LIMDEPTASLTAKEVKDLLDVVIKLKSKGISIIFVSHKLQEVMSVSDSVLVLKDGNMV
                     GQYPIREMNEKRLGFLMTGLEIDYKRLDLPDFLQRKTVLEVRNLTLPHQYQEINFSLR
                     EGEIVALTGLLGSGRTELCLSLFGITQPQSGDILLNGEKVIFNTNRDAIAKGIAYVSE
                     DRMHTGLIMAESIHHNIISTIFHKITDKLQIIQSSKAYASSQALIDSLKIKVSDSDLP
                     VNTLSGGNAQRVSIAKWLAIDPKIIILDAPTIGVDIANKEGIFQIIKSLAQQGIAVIF
                     VTDEVEETYYNSHRVIVMKKGRIVDEILPAYSSEKSIAEVIYENQQ"
     gene            4941..5900
                     /locus_tag="PXD66_001083"
     CDS             4941..5900
                     /locus_tag="PXD66_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL6319394.1"
                     /translation="MKISNNHILSSTLLILILLLTLFTRDFATIENLYDVVNNYAMLM
                     ILACGLFVVLLSGGIDISFPAITIISQYVMVTLIGKYDVGFAGVFVISSTIGILLGFI
                     NALFVNRLSVPSIIITISTLNIFYGGLLYFTKGVWLYEYPEWFTQEIVFFKQITAEGV
                     EFGLSFQALMAILAVILTGLITNKLTIGRKIYALGGNPDATSRIGFNLLFLHLFAYGY
                     MGFMAGLAGIVQSYTVQSVAPDSLLGYELTVLAAVVLGGASLSGGRGTLLGTVMGVML
                     LAIIQNGLNLMNVSSYWQTIITGIIIILSISATALSQRKKQGA"
     gene            5903..6853
                     /locus_tag="PXD66_001084"
     CDS             5903..6853
                     /locus_tag="PXD66_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL6319395.1"
                     /translation="MKSKLDPTIFYLLALFAVAVIAFSIFIPDIFWSVSNFQSMASQI
                     PVLGILTLAMALPMLTGGINLSIIAGMNACSLIIAYFITQYTGISWLFIAILLSLCCA
                     LAIGLLNGTLIAIIRVSPILATLGTMTLINGINILVTNGSTIANFPETMLSINASAFL
                     WIPLPILIFLFLAGLIWFFLEKTAMGKAVYFIGNNEKATYYSGINTKKVLIWVYVISS
                     ILCVFAAILMMSKLNSAKASYGDSYLLISILAAVLGGINPDGGKGKLLGIMLALVLLQ
                     VIESGLNMLGISSYITMILWGSLLLFFIFLQKNRLFRLTQ"
     gene            6866..8461
                     /locus_tag="PXD66_001085"
     CDS             6866..8461
                     /locus_tag="PXD66_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HDL6319396.1"
                     /translation="MQNEKQLIEQGNTVIGIELGSTRIKAVLISSDGTILATGGADWE
                     NRLIDGIWTYHQNEIWEKLQAAYMDLSKTVKEKYHTTIRTAKALGISAMMHGYLPFDK
                     HGNPLVAFRTWRNNITLTSSEKLTALFQYPIPQRWSIAHLHQALLNQEPHLPEIDYLT
                     TLSGYVHWQLTDHKVLGIGDASGMFPIDTELKSYNPTMLAQFDRAIADYRMPWKLEAI
                     LPQVRVAGEQAGKLTEKGAKLLDPTDNLQAGIPLCPPEGDAGTGMIATNCIKEKTGNI
                     SAGTSAFAMIVLEKALSKAYSELDIVTTPAGKLVAMAHANNCTSDINAWVNLFGECLS
                     VFGVNICTEELYEMLFLHALQGDADCGGLLSYGFHSGEHNVGLSEGCPVFLHPTNAHF
                     NLANFMRTHLYTAFGAMKLGMDILINQEKVEISRILGHGGIFKTEGVAQKILSSALNI
                     PLATASTASNGGAWGIALLADFLSVSDRYSLEEYLDNCIFNTTKLNLVQPDKTMSEGY
                     ERFMQRYKQGIPVVEKALFLNTD"
     gene            8691..9893
                     /gene="yedE"
                     /locus_tag="PXD66_001086"
                     /pseudo
     CDS             8691..9893
                     /gene="yedE"
                     /locus_tag="PXD66_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005824242.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
     gene            9929..10198
                     /gene="yedF"
                     /locus_tag="PXD66_001087"
     CDS             9929..10198
                     /gene="yedF"
                     /locus_tag="PXD66_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007241376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HDL6319397.1"
                     /translation="MAFTVVQKLDKDPKDITPDYTLDTLGEPCPYPAITMLETMPQLQ
                     KGEILELLSDCSQSINNIPIDTKNHGYTLLSVEQNGTKLRYLIQR"
     gene            10298..10642
                     /locus_tag="PXD66_001088"
     CDS             10298..10642
                     /locus_tag="PXD66_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDL6319398.1"
                     /translation="MNKYALLAIFWFIIGIYALLFRESTNDIPPFPHFDKVSHFALFF
                     IQIWLSANAFIRSKKGIPYFGLIIFALLYAFTSEWAQATFTSTREGSWLDGLADMLGA
                     SLSLLLVKYCKK"
     gene            complement(10705..11652)
                     /locus_tag="PXD66_001089"
     CDS             complement(10705..11652)
                     /locus_tag="PXD66_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HDL6319399.1"
                     /translation="MNRITQILNIKHPIVQAPMSWLTDAHLVASVAEAGGLGFLAPHA
                     GQTTNPTSNEEVLDRMRNEIRKVKALTDKPFGVPFILSYDFSLIPLMVDLFIEERVPV
                     VLDNGWLDPEIYAKFKAAGIKIICRLPNPNLADALKAQALGADILVLTGFDEGGTLPM
                     KEVGSFNVLAEFVGKVDLPIMLAGGIADKKAVQAALTLGAEGVWIGTAFIATEECRAS
                     EKVKNWIVEANANDLLLFRTEPHYYRSLPTGLAKKCFEMSENGATRSEIAKVMNAGTG
                     MRLGMLEGDFDNGYVSVGNGISQINKIKSVKELIDELMA"
     gene            11835..>12479
                     /locus_tag="PXD66_001090"
     CDS             11835..>12479
                     /locus_tag="PXD66_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319400.1"
                     /translation="MSIRWENIKPLQGSQNSAFEELVCQLARQEFKSQGKFTRISAPD
                     GGIEAMCELPDGSVCGWQAKYFLSSFSSSQWQQIEDSFKESLKNYPKLTKYYVCVATD
                     RANANVSGKKSFLTKWEEYTQRWKDFAQSQGREIEFEFWGSFELSDLLSKPENAGKKF
                     FWFNESELSDKWFEQYNQQAINNLGVRYTPEINVDLPIAMQLESLARTQIFKKHL"
BASE COUNT         3677 a         2437 c         2448 g         3917 t
ORIGIN      
        1 gcgttttatt tacaatccaa atatcgccat tcattatttg gagagttcta tggatatcgg
       61 tattgttaaa tggtttaaca atgtaaaagg atttggtttt attacatcgg atgtatgtga
      121 aggagacatt tttgctcatt tttctgaaat tcaagcagaa ggttatcgct ctttaaaagt
      181 cggccaaaaa gtacagtttg aattagttca aggagagcga ggtgcctcag cctcaaatat
      241 cacgcctata agcgaaaaaa cgttataatc cgtcgttaat aaaattattg ttataaccat
      301 actttattag attttaaagc tcagctttta cagctgagcc atttttgctt tttgaatatg
      361 ccttcctctt atcaattagt cttaattaac ctactttata tcttcggtat tggtctaaat
      421 attgtactgc ttaggtttct cagtttgcat tttgacccat taaataataa cggtatacgt
      481 tttcttgttg gtgggttcac cttattattt ttcattttat ggagattccg cacatcgctg
      541 tctcagctga ttaaaactcc aaaattgatt gcgatttctc tatttgtcgg gttaatgatg
      601 tccgcaaata tgtatttttg gctaaaaggg gtcaaaatta caaatgctgt taccgtctct
      661 atttttgggg tattagctat gccatttggt gtgattattg ccgccatatt ctttcaagat
      721 gaaagagaac gcattaaaag caaagctttc tggttcggct caattttaac gatcatcggt
      781 tcactcggat ttatttggca cgggaaaaca ctctcgattg gcgaaggatt tattcttggt
      841 tctgtgttct tatttttgtc gatcattatc cgtaatatac aaaacctagt tgtaaaattt
      901 gctcgagaac tgaacgtatt aaccctaagt tgtgtaacat ctttaactac ttcactctcg
      961 agtctattac ttagccaaca agcaggtaaa tttaatgagc tttttactgc accaaactgg
     1021 ctactattct ctctgatggt tgctggtatt tatgcgatta tgatcggaat ggtattaacc
     1081 tttcacatta tccaaaccca aggactgata acctaccaag tattggaact attaatgccg
     1141 gtttcaacag ccatcattgc ttatatttta ctggacgaac gtatttccat tgttcaaatg
     1201 ggctttgctt gtattgtgat tttaggctca agtatcgcct taaagttagt aaaatatcct
     1261 aagcactatt aggatctttg ctaattaata ttggtaaaat tacatttggt ttactaatct
     1321 gagcgttcat tcattagtaa tgataaacaa gcggttattt tttgttattt tttggtaaat
     1381 actgatagtc atgtataaaa acctttcttt gctttcaccg acttatccat ttgaccttga
     1441 attcatcgcc ggtcttactc aatgtgagaa aggtaactat cttgattcta ttgtcgatag
     1501 gcaacaagga atgcacggtt atatgcttca attaaccact tttggtcgtg gttctgtttt
     1561 tgatggtaag cacgttttta ccgctcatcg agggcagtta ctcctcttta cacccaatgc
     1621 cgttcaacac taccaccgtc acccggaaag ccaatattgg cactacaaat gggtctattt
     1681 tcagccgaat cctaaatgga ataaatggct gaattggtca gatatggaag aaaaaatagg
     1741 acgtatcacc attagcgatg atcgttactt tcaggaaatt agccaacttt tcagtaaaat
     1801 tgaattagaa cttaaatcca gtaaattttt taaagaagat attgcaacaa gtttgttgga
     1861 atacttactt atgaaatgta tttccgccga gaaaattgag gttattcctg tcattgatca
     1921 acgcattttg acagtttgtg atttaatctt aacgaatttg gctaaaaatg agagcttaga
     1981 agcaatggca gaaaaagtat ttttatcgcc atctcgcctc tcacatcttt tcaagcaatc
     2041 gctggggatc agcttagtgc aatggcggga attacaacgc atttccgaat ccaaaaaatt
     2101 gctttatttc tctaacatct ctatctctaa tattgcgaaa tccttaggct atgaggattc
     2161 tctctacttt tccaaaattt tcaaaaagca taccgggctg tcaccctctg agtttagaac
     2221 cttagaacgg cgtaaagaca gtgaaatgtg atcttgatct cactttactt tgcatagcag
     2281 aattttccat atcgataaca aaaagttacc tgtttttctc tcattcaaaa gcataagata
     2341 caaacagctt taatagtgct gtttctatta gagaagggat atcaagctct gtgagtgata
     2401 ttcagtgaaa taacttctta tgtatgaggg aagaacaatg aaaaaaccaa cgttcaagca
     2461 cgcattattt tcaagtattt tagctttagc ctgttacggc tcggcatttg ccaatgatgt
     2521 tgttaatatc tccaaaattg acggtatgcc gtggtttaat cgtatggcag agggtgttgt
     2581 gcaagcaggc aaagataaca atatcaatgc ttatcaagtt ggtccatcaa ataccgatgc
     2641 accacaacaa gtaaagttaa ttgaagattt aattgcgaaa aaagttagtg ctatttccat
     2701 cgtccctaac gatgcaaatg ccttagaacc ggtactgaaa aaagcgaaac agaacggtat
     2761 tgtggtatta accaatgagt cagtcggaca acctagtgcc gattgggata ttgaaattat
     2821 cgataatgca caatttgcag cagactatgt agaagaagta gcaaaagcta tgggcggaaa
     2881 aggcggctat gtgatttatg ttggtggttt aaccgtgcct caacataatc tgtgggctga
     2941 tttattcgtg aaataccaga aagagcatta tccggatatg tttgaagtga ccagcagaat
     3001 gcctgttgcc gaaaatatta atgatgcacg ccgtaccacc attgatttag tgaaagccca
     3061 ttccgatctt aaagcggtcg tttcttttgg ttcacaaggt cctattggtg cagggttggc
     3121 agtgaaagaa aaacgcttaa aaaataaatt aaatgtcttc ggaatgatga ttccttccca
     3181 agcagccaat ttaattaaaa gcggtgatat tacaatggga attacctatg acccggccaa
     3241 tgcaggttat gctttagctg ctgtcgctgc caaagtatta aaaggcgaaa aaattgagga
     3301 tggattagag atcccaacgg tcggtaaagc caaagtagat agtgagaaaa aactacttca
     3361 attccacaat gtgctgaaag tcaccaagga aaatatcgat aaattatatt aatttgcttg
     3421 aaatatgggc tgatagccag cccatatttt ccctcaggag gcacttatgg catttatttc
     3481 gatgcggcat atcagtaaaa cattccacgg cataaaagca ttaaatcaag tcaatctctc
     3541 attagattat ggcgaagcac tttgtcttgc cggtcaaaat ggctgtggaa aatcaacctt
     3601 gatcaaaatt ctttccggcg tttatcaacc ggataaaggg gctgaaattc aaattggtgc
     3661 aacccaatat accaagctga caccacagga atcgattgat aaaggtattc aagtgattta
     3721 tcaagattta gccctcttcc ccaatttaac cgtggcagaa aatattgcca ttaacctgca
     3781 ccgaaaattt ggctgggtga gtcaagatca cattcatcag gttgccctca ctgctattca
     3841 aagcattaat gcaaatctag acctgaatgc aatattagaa gatttaccga tcgctcaaca
     3901 acagttagtc gctatttgtc gagcgttagc acaaaatgcc agattattga ttatggacga
     3961 gccgactgcc tcacttactg caaaagaggt caaagatctg ttagatgtcg tcatcaaact
     4021 taaaagcaaa ggcattagca tcatctttgt cagtcataaa ttgcaagaag taatgagtgt
     4081 ttccgatagc gtattagtgc tgaaagatgg caatatggtt gggcaatatc ctatccgtga
     4141 aatgaatgaa aaacgccttg gtttcctaat gactgggctg gaaatcgatt acaagcggtt
     4201 agatttgccg gattttttgc aaagaaaaac cgtattggaa gtccgcaatt taacgctgcc
     4261 tcatcagtat caagagatta acttttcgct acgagaaggg gaaattgttg cccttacagg
     4321 attgctcggt tcagggcgta ctgagctttg cctaagttta ttcggcatta ctcaaccaca
     4381 atccggcgat attttattaa acggtgaaaa agtgatattt aacactaacc gtgatgccat
     4441 tgccaaaggg attgcttatg tatcggaaga tagaatgcac accgggctga ttatggcaga
     4501 gtcgattcat cacaatatta tttcgactat ttttcacaaa atcaccgata aattacagat
     4561 tattcaatcc tcgaaggctt atgcaagtag ccaagccttg attgattcat taaagattaa
     4621 agtatccgat tcagatttgc ctgttaatac cctttccgga gggaacgcac agcgagtttc
     4681 gattgcaaaa tggctggcaa tcgaccccaa aattattatt ttagatgccc cgactattgg
     4741 ggtcgatatc gccaataaag aagggatctt ccaaattatt aaatcattgg ctcagcaagg
     4801 cattgcagtt atttttgtga ctgatgaagt ggaagagacc tactacaaca gccatcgggt
     4861 aattgtaatg aaaaaaggtc gcattgttga tgaaatcttg ccggcatatt ccagtgaaaa
     4921 atcgattgcg gaggttattt atgaaaatca gcaataatca tattctttcc agtacgttac
     4981 tgattttaat cctattatta accctattta ctcgtgattt tgcaactatt gagaaccttt
     5041 atgatgttgt aaataattac gcaatgttaa tgattttagc ttgtggttta tttgtggtat
     5101 tgctgtctgg cggcattgat atttctttcc ctgctatcac tattatttcc caatatgtta
     5161 tggtcactct gatcggtaaa tatgatgtag gatttgccgg cgtttttgtt atttcttcca
     5221 ccatcggcat attattaggt tttattaatg ctctatttgt taatcggctt agtgtccctt
     5281 ccattattat tacgatctca acactcaata ttttttatgg cggactactc tattttacca
     5341 aaggtgtatg gctttatgag tatcccgaat ggtttaccca agaaatcgtg ttctttaaac
     5401 agataactgc tgagggagtg gaatttggat tatcgtttca agcattaatg gctattttgg
     5461 cggtgatatt aaccggctta attaccaata aactcactat tggtcgaaaa atttacgctc
     5521 ttggtggtaa tccggatgca acctcccgta ttgggtttaa tctcttattt ctccatctat
     5581 ttgcatacgg ttatatgggc tttatggcag gcttggcagg catagtgcaa tcttataccg
     5641 tacaatctgt cgcaccggat tcactactgg gttatgaatt aaccgtccta gctgccgttg
     5701 tattaggtgg tgctagttta tcaggtggta gaggcaccct gcttggtaca gtaatgggcg
     5761 taatgttact ggcgattatt caaaacgggc taaatttaat gaatgtatcg tcttactggc
     5821 aaaccattat taccggtatc atcattatct taagtattag tgcaacagcc ttaagtcagc
     5881 ggaaaaaaca aggagcataa cgatgaaaag taaactggat ccaacgattt tctatctctt
     5941 agcactcttc gcagttgcag ttatcgcctt tagtattttc attccggata ttttttggtc
     6001 tgtcagtaac tttcaatcta tggcatcaca aattccagta ttaggtattt taaccctagc
     6061 aatggcatta ccgatgttaa caggtggaat aaatctttcc attattgccg gaatgaatgc
     6121 ttgctcatta attattgcct actttattac gcaatatacc ggtatttctt ggttatttat
     6181 tgctatttta ttaagccttt gctgtgcctt agccattgga ttactcaacg gcacattaat
     6241 tgccattatt cgtgtttcac caattttggc aaccttaggc acaatgactt taattaacgg
     6301 cattaatatt ctagtcacta acggttcaac cattgcgaac tttccggaaa caatgttgag
     6361 tattaatgct tcagcctttt tatggattcc gctacccatt ctaatctttc tctttcttgc
     6421 tgggctgatt tggtttttct tagaaaaaac tgcaatgggt aaagcggttt atttcatcgg
     6481 caataatgaa aaagccacct attattcggg tattaatacc aaaaaagtgc tgatttgggt
     6541 ttatgttatc tcctcaattc tttgtgtatt tgcagctatt ttgatgatgt ctaaactcaa
     6601 ttctgcaaaa gcctcttatg gtgattccta tttgttgatc tccattttag ccgcagtatt
     6661 gggcggtatt aaccctgatg gaggtaaagg gaaactatta gggattatgc tcgccttagt
     6721 tttattacaa gtgattgaaa gtggcttaaa tatgctcggc attagcagtt acattacaat
     6781 gattttatgg ggaagcttat tgttattctt tatcttcctc caaaaaaatc gccttttccg
     6841 actaacacag taatggtggt agattatgca aaatgaaaaa caactcattg aacaaggtaa
     6901 tactgttatt ggtattgaat tgggctcaac ccgaattaaa gcggtgttaa ttagctcaga
     6961 tgggacgatt ttagcaaccg gtggggcgga ttgggaaaat cgactgattg acggtatttg
     7021 gacctatcat caaaatgaaa tatgggaaaa actacaagct gcttatatgg atttaagcaa
     7081 gacagtcaaa gaaaaatatc acacaaccat tcgcacagcc aaagcattgg gaattagtgc
     7141 gatgatgcac ggctacttac ctttcgataa acacggaaat ccgctggttg cctttagaac
     7201 ttggcgaaac aacattaccc taacctcatc ggaaaaattg accgcactct ttcaataccc
     7261 aattccgcaa cgttggagta ttgctcattt acaccaagca ttgcttaacc aagagcctca
     7321 cttgccggaa attgattacc tgacaaccct ctcaggttat gtgcattggc aactgaccga
     7381 tcacaaagtg ttagggattg gcgatgcttc cggcatgttc ccgatagata ctgagttgaa
     7441 atcctacaat ccaacaatgc tggctcaatt tgatcgagcc attgccgact accgtatgcc
     7501 ttggaaattg gaagcgattt tgccacaagt tcgtgttgct ggtgaacaag cgggcaaatt
     7561 aaccgaaaaa ggtgcaaaat tgttagatcc aaccgataat ttgcaagctg gcattccact
     7621 ttgtccacct gagggtgatg ccggaacagg tatgattgca accaactgta tcaaagaaaa
     7681 aaccggtaat atttctgccg gcacctccgc atttgcaatg attgtgttag aaaaagccct
     7741 atccaaagcc tattcagagc tggatattgt gacgaccccg gcaggtaaat tagtggcaat
     7801 ggctcacgct aataattgca cctctgatat taatgcgtgg gtgaatttat tcggcgaatg
     7861 cttatcggtt tttggcgtaa atatctgcac cgaagaactt tatgaaatgt tatttctcca
     7921 tgccttgcaa ggcgatgcag attgtggagg gctattatcc tacggcttcc actccggtga
     7981 acataatgtc ggtttatccg aaggctgtcc ggttttcttg catccaacga atgcccattt
     8041 taacttagca aactttatgc gaacccatct ctataccgct tttggtgcaa tgaaattagg
     8101 aatggacatt ctcattaacc aagaaaaagt ggaaatttcc cgtattctcg gtcacggtgg
     8161 tatatttaaa acagaaggtg ttgcacaaaa aattctgtct tccgcactga atattccatt
     8221 ggctaccgca agtactgctt caaatggcgg agcttgggga attgcattac tggcagactt
     8281 tctcagcgta tcagaccgtt acagtttaga agaatattta gataattgca ttttcaacac
     8341 caccaaactc aatctggttc aaccggataa aacaatgagt gaaggttatg aacgctttat
     8401 gcaacgttat aagcaaggca ttcctgtggt agaaaaagcg ttgtttttaa atacagatta
     8461 aataattata atttaagtat cacaagcggt tatttttgca aaaaacgacc gcttgttatt
     8521 tacatttgta caaaaaatac ctactataaa gacgcatttg taaaatgtgc attgatatat
     8581 cgcaagtttt gtgtatagga aatgaactaa ttactgtgtc aatcagtccc tttcttcatc
     8641 gttataccct cttgcaaatc cgattaatct atccttagtt aaggtgtttt atgttagcga
     8701 tttggcaaag ttttaaacaa aattatctaa tcaaatattg gaatcctgtt gcagcagtga
     8761 tcgcagctgg tattatttca gcttattatt tcggcattac tggcacttat tgggcggtta
     8821 caggggaatt tacccgttgg ggcggtcatc tgttaaatgc tttgggtgtt gatgtttcga
     8881 actggagcta ttttcaactc atcggcttag acggcaccat ctttacccgc attgacggtg
     8941 tgatgattct cggtatgttt gccggctgtc tttctgccgc attatgggcg aacaacgtca
     9001 aatggcataa tcagccacat aaaaaacgga ttatccaagc cttgattggc ggtgcgttag
     9061 ccggtttcgg agcccgttta gcaatgggct gtaacctcgc ctctcttttt accggtattc
     9121 cgcaattctc cgtccacgct tggtttttca cctttgcgac cgccatcggc acttactttg
     9181 gggtaaaagt gactttatta ccgatgttta gagtgaaatt agagctgaaa aaaggggcag
     9241 caaaaatcaa agaaaacgat ccgaaacaag ctaatcgccg tttttgggtt ggtatgctca
     9301 tttttatcac ttatctttct gcttcccttt atgtaatgca atcctcaatc aaacttggtt
     9361 ttgcaatgct ttgcggctta gcctttggct tattgattga acgagcccaa atttgcttta
     9421 cctccgcttt ccgtgactta tggattaccg gacgggctta tatggcaaaa gcaattattt
     9481 tcggcattat tgccggcaca atcggcgtgt tctcttatat tcaattaggt gtttcaccta
     9541 aaattttgtg ggctggccca aatgccatta tcggcggatt attattcgga tttggcattg
     9601 tgcttgccgg cggctgtgaa accggctgga tgtaccgttc aatggaagga caggttcatt
     9661 ttatgtgggt cggcgtaggc aatgtagtcg gttcaaccct gctggcttac tactgaggcg
     9721 acttagctcc ggtgcttacc cttgattatg aaaaaatcaa cctactcaaa gaatttgggc
     9781 cggttggcgg attattcatc aattatggct tattaattct ttgcttaatc gctgtagttt
     9841 ggtgggaaaa acgcttcctt gccaaagcga aagcaaaatt agcaactgcg taatcttttt
     9901 aacttcaacc aacaagagga aaatgactat ggcattcacc gttgtacaaa aactagataa
     9961 agatccaaaa gacatcacgc ctgattacac cttagatacc ttaggcgaac cttgccctta
    10021 ccctgcgatt acgatgttag aaacaatgcc acagctgcaa aaaggagaga tattggaact
    10081 gttaagcgat tgttcccaat cgattaataa tattccgatt gatactaaaa atcacggcta
    10141 taccctatta agcgtggaac aaaacggcac taaattacgc tatttaattc agcgttaatt
    10201 gcaaaaaatt cgcacaaaac gaccgcttgt cgtaaaatag caactattta agagccttgt
    10261 aacgcaaggc tcttttattc taagaggtaa aagaaaaatg aataaatacg ctttacttgc
    10321 aatattttgg tttattattg gcatttatgc tttacttttt cgtgaaagca ctaatgatat
    10381 accgcctttt ccacattttg ataaagtaag ccattttgcc ttattcttta tccagatctg
    10441 gttatctgca aacgccttta tccgttcaaa gaaagggata ccttattttg ggctaataat
    10501 ttttgcctta ctctatgctt tcaccagcga atgggctcag gcaaccttca cctcaacccg
    10561 agaaggttct tggcttgatg ggctagcaga tatgctagga gcctcacttt cattattgct
    10621 ggtaaaatat tgcaaaaaat aaagcataaa agaccgcttg ttatatggtt tagccatact
    10681 acaagcggtc attttttacc aaaattatgc catcaactca tcaatcaatt ctttcacact
    10741 tttgattttg ttgatttgtg aaatgccatt gcccaccgac acatagccat tgtcaaaatc
    10801 cccttctaac atacccaaac gcattcctgt accagcgttc attaccttag caatctcact
    10861 acgagttgcc ccattttcgc tcatttcaaa gcattttttc gctaatccag tcggcagtga
    10921 acggtagtaa tgtggttcgg tacggaataa cagcagatca ttggcatttg cctccacaat
    10981 ccagtttttc actttttcgc ttgcacggca ttcttcggtg gcgataaatg ccgtgccaat
    11041 ccacacgcct tcagccccaa gcgttaaggc agcctgcacc gcttttttat cggcaatacc
    11101 accagccagc attatcggca aatccacctt gcccacaaat tccgccagca cattaaaact
    11161 gcctacctct ttcatcggca aagtaccgcc ctcatcaaaa cccgtcagca ccaaaatatc
    11221 tgctcccaat gcttgggctt ttaaagcgtc cgccaaattg ggattaggca aacggcagat
    11281 gattttaatg ccagcagctt tgaatttggc gtaaatctct gggtcaagcc agccgttatc
    11341 cagcaccaca ggcacacgct cttcaatgaa taaatccacc attaacggaa tcagcgaaaa
    11401 atcataagac aaaataaatg gtacgccaaa aggcttatcg gtcaatgcct taaccttacg
    11461 aatctcgtta cgcatacggt caagtacttc ttcgttggaa gttggattgg tggtttgccc
    11521 tgcgtgtggg gctaaaaaac caagcccacc agcctcggca acgcttgcta ccagatgagc
    11581 gtccgtcagc caagacattg gcgcttggac gatagggtgc ttgatattta agatttgggt
    11641 aatgcggttc attggatttt cctgtttgaa taaaaagaat tggacgtagt ttaatggttt
    11701 tggatttgag agggaatgcg tgaaaatggg aaggtggttt tcggatttgg aaaattatca
    11761 atgctaaaat acttgaaata cacacgccaa tcctcatatc aacaaaattg aatatttaat
    11821 tatggtaaaa gaaaatgtct attagatggg aaaacatcaa gcccttacaa ggttctcaaa
    11881 atagtgcttt tgaagagcta gtatgccaat tggcacgcca agaatttaaa tctcaaggaa
    11941 aatttactcg aatttctgca cctgatggtg ggattgaagc aatgtgtgaa ttgccagatg
    12001 gttccgtatg tggttggcaa gccaaatatt ttttaagctc attttcttca tcacaatggc
    12061 aacaaattga agattccttt aaagaatctt taaaaaacta tcctaaacta accaaatact
    12121 atgtttgtgt ggcaaccgat agagcaaatg ccaatgtatc tggaaagaaa tcatttctta
    12181 ccaaatggga ggagtatacc caaagatgga aagactttgc tcaatcacaa ggtcgtgaaa
    12241 tagaatttga attttgggga agttttgaat tatctgactt attatcaaaa cctgaaaatg
    12301 caggcaaaaa attcttttgg tttaacgaaa gcgaattatc tgacaaatgg tttgaacaat
    12361 acaatcaaca agccattaat aatcttggtg tgcgttatac acctgaaatt aatgttgatt
    12421 tacctattgc aatgcaatta gaaagcttgg caagaaccca aattttcaaa aaacatctg
//
LOCUS       DANLXF010000062        12419 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.062, whole genome shotgun sequence.
ACCESSION   DANLXF010000062 DANLXF010000000
VERSION     DANLXF010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12419
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.062"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(73..657)
                     /locus_tag="PXD66_001091"
     CDS             complement(73..657)
                     /locus_tag="PXD66_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoxide reductase heme-binding subunit YedZ"
                     /protein_id="HDL6319401.1"
                     /translation="MLTWLRILIHLGCLLPLVWVAQLLYTGNETILGADPIKELEHFL
                     GYGAIVIFCIMFLLGIALQYLKKNQYQILRRPLGLWAFVWAALHISSYLLLELSLDVK
                     LFFSELAGRPYLILGAAAFFILSIMAITSLPMLKRKLGTRWGTIHQWAYPALVLAMVH
                     YYWSVKSVTFDPIIIGVLVLFIVAHKIQAKFAKK"
     gene            complement(657..1610)
                     /gene="msrP"
                     /locus_tag="PXD66_001092"
     CDS             complement(657..1610)
                     /gene="msrP"
                     /locus_tag="PXD66_001092"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HDL6319402.1"
                     /translation="MKKLTIQDVTPETIFYQRRKIITALGLGIAASALPKVSMAETQR
                     HLQSLQFKPDPNKGLELTPENKVIGYNNFYEFGVDKSAPAKNAANFKTDPWTLEIGGE
                     VENPFSLNHQQLLNTFPLEERIYRFRCVEAWSMVVPWIGFELARLIEMAKPTSKAKYV
                     VFHTLYDPAQMPGQKNRLLGGGIDYPYVEALRLDEALNSLTLLSVGLYGKTLPPQNGA
                     PIRLVVPWKYGFKSIKSIVKITFAEKRPLTTWESLAPNEYGFYANVNPNVDHPRWSQA
                     SERVIGAGGLLSVKRQPTLMFNGYENEVANLYRGLDLKVNY"
     gene            1719..2285
                     /locus_tag="PXD66_001093"
     CDS             1719..2285
                     /locus_tag="PXD66_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL6319403.1"
                     /translation="MKSLSSTQIEEIRKQMVKFATLQLADKDLAEDVVQDALTNAYKH
                     SASFRREAALKTWIFAILKNKILDLLRTRKRHIPVSSLCEEEESPSAFFDRHDHWLEH
                     QDVTEWRGVAQSTYQKEFWAIFDICLNKLPAQQARVFMMREHLEMSSAEICQECEISL
                     SNLNVLLHRARLRLQECLSRNWFREESK"
     gene            2282..2467
                     /locus_tag="PXD66_001094"
     CDS             2282..2467
                     /locus_tag="PXD66_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HDL6319404.1"
                     /translation="MKCRQATELISQSCDEKLRLQQHMRLQLHLLVCPKCRNFNKNTK
                     KLGKILRAYCNDKDAPF"
     gene            2497..2781
                     /locus_tag="PXD66_001095"
     CDS             2497..2781
                     /locus_tag="PXD66_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319405.1"
                     /translation="MRYWLISLSILLSGCVVPVTPTGQAGIPMPIIIGVSDPLDSKEV
                     HICTLKPFTQEYRSEHSSRGRAKLNVQKQCRANHSEMFCEEKDIVCKTYD"
     gene            2885..3928
                     /locus_tag="PXD66_001096"
     CDS             2885..3928
                     /locus_tag="PXD66_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL6319406.1"
                     /translation="MQPINIAIAGAGFSSRVFHIPFLKQDARFNIVKVYERTSNNAAE
                     WLPNAETVRDFNALLTPEVDLVLITTPNQTHYAMVKESLLAGKHVLVEKPLVATVAEA
                     EELAELAKRQGKVLYVYQNRRWDSHIATAKSLMEQNLLGELVDCEIRFDRYAKEKNVK
                     VWKESGELGTGLVYDLGVHLIDQALYLFGKPEAVYADVRYQHEGALVDDNFDIQLYYA
                     NGFKVVLKASRYAREPSPTFVLHGKLGSYLKQTPDNQEDLLKNGVQPIGKDWNIEEKE
                     NWGILHTEVEGNVVRQPYRNAEVSYQNLFDDLYQAIAHNAEPIIKLEDVIFVLKVIES
                     VFESAKLGQKIYL"
     gene            4168..5274
                     /locus_tag="PXD66_001097"
     CDS             4168..5274
                     /locus_tag="PXD66_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HDL6319407.1"
                     /translation="MNKLIASLVASSCATAFAAPVQQNNFHTTTHTYEFTQSYDLVVP
                     QGSKGTTNLWIPLPSDTDYQTVKSIEFEGSYKNAYITENNAYGAKTLFATWDTDAPKR
                     DLKVKMVIQTQDREPMAQGALANYQMPEKITYSVDVLEYLKPTAHIKTDGIVKEYADK
                     IVGNEQNPLKKAELIHKWIVDNMERDNSVLGCGDGDVEKILTTKVLKGKCTDINSVFV
                     ALARASNIPAREVFGIRLGQAVKMGQYSKTAFGSSKDGVANESGGQHCRAEFYLAGFG
                     WVPVDSADVAKMRLAEKKDVDDADVQAVSKYLFGNWEMNWVGFNHGRDFDLYPQPELT
                     PLNNFGYPYAEVGGDPLNSFEAKEFGYEFISKEL"
     gene            5249..5617
                     /locus_tag="PXD66_001098"
     CDS             5249..5617
                     /locus_tag="PXD66_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319408.1"
                     /translation="MNSSLKSYNHKFITMCVTAIVSAVTSTFCCIGPLVYLVFGISSP
                     WLMELNQLEFLQLPMLLISLAAFGYGFWLLNFSNKIICTKYFSRRTLVILYWVVSVVV
                     MFFLTYPYVLPYILEMWSGE"
     gene            5619..5927
                     /locus_tag="PXD66_001099"
     CDS             5619..5927
                     /locus_tag="PXD66_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDL6319409.1"
                     /translation="MKKYIATMLLAFGLVHSSYAQEKTAQIETKQAEKTVVLHIPEMY
                     CQLCVYLVNKELRAVEGVISTKASMKERKATVVVKESVDNQALIQAVGNLKYTAKVIE
                     "
     gene            6165..6719
                     /gene="srlA"
                     /locus_tag="PXD66_001100"
     CDS             6165..6719
                     /gene="srlA"
                     /locus_tag="PXD66_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005725059.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDL6319410.1"
                     /translation="MIEAITHGAEWFIGLFQKGGETLVGMINGILPLLISLLVVMNAL
                     IKFVGQDRIERLAQRSAGNPISRYLILPVIGTFVFCNPMTLSLGRFLPERYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLGLPLGPLAISYLLVGMFTNFFRGWI
                     TDFTTAFFEKRMGITLDREVHLSH"
     gene            6750..7751
                     /locus_tag="PXD66_001101"
     CDS             6750..7751
                     /locus_tag="PXD66_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDL6319411.1"
                     /translation="MSNKAIYIEKGNGGWGGPLTLPVVEGKKVLYMTGGTRPAIVNKL
                     VELTGWEAVDGFKEGEPPAEEIGIAIIDCGGVLRCGLYPKRRIPTINIHSTGKSGPLA
                     EFIVEDIYVSAVKENNIRLIDADGSVPVATEQAKPTSSTTATAKEYDSSKKITEQSDG
                     LLAKIGMGMGSVAALFFQAGRDTIDTVLKTILPFMAFVSALIGIIIASGLGDVIAHGL
                     TPLANSPIGLVTLALICSFPLLSPFLGPGAVIAQVIGVLVGTQIGLGNIPPHLALPAL
                     FAINAQAACDFIPVGLSMAEAKQDTVRVGVPSVLVSRFLTGAPTVLIAWAVSAFIYQ"
     gene            7796..8194
                     /gene="srlB"
                     /locus_tag="PXD66_001102"
                     /gene_synonym="gutB"
     CDS             7796..8194
                     /gene="srlB"
                     /locus_tag="PXD66_001102"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDL6319412.1"
                     /translation="MHRLSKRENIYMTIIYQTTFTKIGNFAQESLTDDMLITFKQGAP
                     ADLQDYCFIHNPSELSSPLEVGDIAEFDGVAYPITAVGSVASENLSALGHITFRFDGA
                     NDAEFPGSVHVIGTPPQGLTENSTLIIKRD"
     gene            8242..9030
                     /gene="srlD"
                     /locus_tag="PXD66_001103"
     CDS             8242..9030
                     /gene="srlD"
                     /locus_tag="PXD66_001103"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HDL6319413.1"
                     /translation="MNNEKKVAIVVGGGQTLGAFLSEGLADAGYRVVVADLNGENAQK
                     VAADINAKQGEGNAIGVQVDAADEPSVEALAKATDTVFGRADVLVYSAGTAKASPIWD
                     FNLNDFDLSVKVNLTGYFLTAKHFSRLMIRDGIKGRIIQINSKSGKVGSKYNSGYSAA
                     KFGGVGLTQSLALDLADKGITVHSLMLGNLLKSPMFQSLIPQYAAKLGIPESEVEQVY
                     IDKVPLKRGCDYQDVLNVLKFYAGEEAAYCTGQSINITGGQVMF"
     gene            9199..9579
                     /gene="gutM"
                     /locus_tag="PXD66_001104"
     CDS             9199..9579
                     /gene="gutM"
                     /locus_tag="PXD66_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HDL6319414.1"
                     /translation="MDSITLLIILAMIMWLLQAVLGWLQIKAFNQAFMEISKKGKVTV
                     GRNSGRFNPKSVIVLAFDDEKKVVDSLCMKGFSVFARPQKLASVIGLSLDQIVPEKIF
                     PSDKRSQFALKVAISSGYSCSDQK"
     gene            9642..10289
                     /gene="hxpB"
                     /locus_tag="PXD66_001105"
     CDS             9642..10289
                     /gene="hxpB"
                     /locus_tag="PXD66_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HDL6319415.1"
                     /translation="MQINSVIFDMDGVMIDSEPQWAEAQIAVLADLGINITEQDCEQL
                     TRGKRIDELSDTWIKKFQLSVSNQALSEAILAHGCAAIEQHGKALTGLYELLDFLKSN
                     QIKLAVATSSPPVVIKAVFDRLKLWDYFPIRCSAMDEEYGKPHPAVYLSAVKKLGIEK
                     QECIVIEDSVTGLIAAKAANLTTFLVNTDYQNEKFSIADKRMASLLDVIEKLKQH"
     gene            10332..11129
                     /locus_tag="PXD66_001106"
     CDS             10332..11129
                     /locus_tag="PXD66_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005725056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor"
                     /protein_id="HDL6319416.1"
                     /translation="MKPFERRQQILAYLAQHGRTEVDVLADYFKLTGATIRKDLTSLE
                     EDKKVLRTYGSVVLIDEEILEREEKINKEDKDDKPLAQKTGINLQQKQAIGQKAAELI
                     NEGNSIIFDAGSTVLQMIPHLKAFDNLSLMTNSLTIFNEILKLNKSYNLLISGGTFRE
                     KSASFHGYFAESAFIGSNFDILFIGTDGLDLDVGLTTFNEVYKVSSLMCDAANKIVLL
                     ADSSKFGRKSPNIVCGLEKIHTIITDEGLSQEMREQIKAKGIELLVV"
     gene            11140..12075
                     /locus_tag="PXD66_001107"
     CDS             11140..12075
                     /locus_tag="PXD66_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HDL6319417.1"
                     /translation="MNHLDSAKETLNLYAQQIITLNHRLSEEFNEVVNMILACQGRVV
                     VGGIGKSGLVGKKMVATFASTGTPSFFLHPTEAFHGDLGMLKPIDMVILISNSGETDD
                     VNKLIPSLKNFGNKIIAMTGNPYSTLGRNADVILNIGVEREACLNNLAPTSSTLVTMA
                     LGDALAIALMKARDFRPEDFARYHPGGSLGRKLLNRVRDVMVRKVPTASLDTTFTECL
                     SVMNEGRMGVAVIMQDDKLEGIITDGDIRRTLAKFGAESLNKTADEIMTRNPKTINDT
                     EFLAKAEDLMKELKIHSLIAVNDEGKVTGLMEFSS"
     gene            complement(12119..12355)
                     /locus_tag="PXD66_001108"
     CDS             complement(12119..12355)
                     /locus_tag="PXD66_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL6319418.1"
                     /translation="MFTRAAKQRNNVKQYQLWQHHNQPITIYSQKFFDEKLNHIHNNP
                     IVSGFVCEAFEWKYSSARNYANNLPVLLDIDICQ"
BASE COUNT         3741 a         2315 c         2688 g         3675 t
ORIGIN      
        1 gttttagtcg ttatctagga cagcccctta ttttttgtat gtagtttgac tgctgcaagc
       61 ggtgtgattt tgctattttt ttgcaaattt agcctgtatt ttatgagcaa caataaacag
      121 cactaagact ccgatgataa ttggatcgaa tgttacgctc tttaccgacc aataataatg
      181 taccatcgcc aatactaatg caggataagc ccactggtga atcgtccccc agcgtgttcc
      241 taattttcgt tttagcatag gcagcgaagt tattgccatt atgcttagaa tgaaaaaagc
      301 agctgctcct aaaatcaaat atgggcgacc ggcaagctcg ctaaaaaata attttacatc
      361 aaggcttaat tctaacaata gatagcttga aatatgtaga gctgcccata caaacgccca
      421 taagcccaat ggacggcgta aaatttgata ctggtttttc tttaggtatt gtagggcaat
      481 accaagtaga aacattatac aaaaaatgac gattgcacca taaccgagaa aatgctccag
      541 ttctttaatc ggatcggcac ctaatattgt ttcattaccc gtatataata gctgtgctac
      601 ccaaactagc ggtaataaac agccaagatg aatgagtata cgtaaccagg ttagcattag
      661 tagttcacct ttaaatctaa gccacggtat aaatttgcca cttcattttc gtagccatta
      721 aacattagtg ttggttggcg tttgacggat aataacccgc ctgcaccaat tactcgttca
      781 gatgcttgcg accaacgtgg atgatcaaca tttggattca cattagcata aaagccatat
      841 tcatttggag caagactttc ccaagtagtg agtgggcgtt tttcagcaaa ggtgattttc
      901 acaatcgact taatactttt aaagccatat ttccaaggca cgactaagcg aataggagca
      961 ccgttttgcg gtggcagggt tttaccgtat aaaccgacgg atagtagtgt taatgaattt
     1021 aaggcttcat ctaagcgtaa agcttctaca taaggataat caatcccacc gcctagcaag
     1081 cgattttttt gccccggcat ttgtgcagga tcatataaag tgtgaaagac cacatatttg
     1141 gctttactgg ttggttttgc catctcaatt aagcgagcta actcaaagcc gatccacggc
     1201 acaaccattg accacgcttc aacacagcga aaacggtaaa tgcgttcttc tagtggaaac
     1261 gtattaagca attgttgatg atttaagcta aacggatttt caacttctcc gccaatttcc
     1321 aatgtccaag gatcggtttt gaagtttgcc gcattttttg ctggtgcact tttatccacg
     1381 ccaaactcat agaaattgtt atagcctatt actttgtttt ccggagtgag ttcaagccct
     1441 ttatttggat ccggtttaaa ctgtagtgat tgcaagtgtc tttgtgtttc tgccatactt
     1501 actttaggca atgcagaagc tgcaatacct aaacctaacg cggtaataat tttacggcgt
     1561 tggtagaaaa tagtttcggg tgtaacatct tgaattgtta attttttcat cgaactatcc
     1621 tttttttatg aattgttgtt attattttgg actcagctga tctagaattc ttacaaaata
     1681 agtgtaattt tttattgggt attccctaaa ggagatatat gaagagtcta tcttctacac
     1741 agatagaaga gattagaaag caaatggtta aatttgccac cctacaacta gccgataaag
     1801 atttagctga ggatgttgta caggatgcct taaccaatgc ctataagcat tcagcctctt
     1861 ttcggcgaga agctgcactt aaaacttgga tttttgctat tttaaagaac aaaattttag
     1921 atttactcag aacacgaaaa cgacatattc cagtcagttc tctatgtgag gaagaagaat
     1981 caccaagtgc ctttttcgat cgccacgatc attggttgga acatcaagat gtaactgaat
     2041 ggaggggggt agctcaatca acttaccaaa aagaattttg ggcaattttt gatatttgtt
     2101 taaataaatt acccgcccag caagcacgag tatttatgat gcgtgaacat ttggagatga
     2161 gtagtgcgga aatttgccaa gaatgtgaaa tttctctctc taacttaaat gtattacttc
     2221 atcgtgctcg tttacgttta caagagtgtt tatctcgcaa ttggtttaga gaggaatcaa
     2281 aatgaaatgc aggcaagcaa cagaacttat ttcacagtct tgtgatgaga aacttcgttt
     2341 gcaacaacat atgcgtttgc agttgcatct tttggtatgc cctaaatgcc gaaattttaa
     2401 taaaaatacc aagaaattag gcaagatatt aagagcttac tgtaacgata aagatgctcc
     2461 gttctgattt attatacatt ttaaaaggag aaaaatatgc gttattggtt aataagttta
     2521 agcatactat taagtggctg tgtcgtgccg gttactccta cggggcaagc cggtatccct
     2581 atgccgatta taattggtgt ctcagatcca cttgattcca aagaggttca catttgtact
     2641 ctaaagccat ttactcagga atatcgtagt gaacatagta gccgaggcag agccaaatta
     2701 aatgtacaaa agcaatgccg tgcaaaccac agtgaaatgt tttgtgaaga aaaagatatt
     2761 gtttgcaaaa cctatgatta gttaaacaag cggtcgtatt ttatctgttt tttgcaaaat
     2821 tttcctaaaa tttgaccgct tgtttattta agcagataac atcaaaattt tatgaggatt
     2881 gtctatgcaa cccatcaata ttgccatcgc aggtgccggc ttttccagcc gagtttttca
     2941 tatcccgttt ttgaaacaag atgctcgttt taatattgta aaggtatatg aacgaacctc
     3001 taacaatgcg gcagaatggt tgccgaatgc agaaacagtg cgggatttca acgctctttt
     3061 aacgccagaa gtggatttag tgctgattac cacaccgaat caaacccatt atgcaatggt
     3121 aaaagagtca ttgcttgcag gcaaacacgt tttagttgaa aaaccgttag tggcaacagt
     3181 ggctgaggcg gaagagttgg cggaacttgc aaagcggcaa ggaaaggtgc tttatgtgta
     3241 ccaaaaccgc cgttgggaca gccatattgc gacggcaaaa tcgctaatgg agcaaaattt
     3301 actgggagag ttggtagatt gcgaaattcg ttttgaccgt tatgcgaaag agaaaaatgt
     3361 taaagtatgg aaagaatcag gcgagcttgg tacaggttta gtgtatgatt taggcgtaca
     3421 tttaattgac caagctttgt atcttttcgg gaagccggag gcggtgtatg ctgatgtgcg
     3481 ttatcagcac gaaggggcat tggttgatga taactttgat attcagctct attatgctaa
     3541 cggttttaaa gtggtgctga aagccagccg atatgctcgt gaaccaagcc cgacatttgt
     3601 attacacgga aaattaggct cttatctgaa acaaactccc gataatcaag aagatttgct
     3661 gaaaaatggc gtacaaccta ttggcaaaga ttggaatatc gaagaaaagg agaactgggg
     3721 aattttacac actgaagtgg aaggtaatgt ggttcgccag ccctatcgaa atgccgaagt
     3781 cagttatcaa aacttgtttg atgatttgta ccaagccatc gctcacaatg ctgagccaat
     3841 tatcaaactc gaagacgtca tttttgtgct aaaagtgatc gaaagcgtat ttgaaagtgc
     3901 aaaattaggg caaaaaatct atttgtaaaa ttttgaggat ttttgaccgc ttgttattct
     3961 ctctgatggc gcaagcgtcc acgcttgtgc ctattgatta ttttagcttt gtttctagta
     4021 caagcgtgga cgcttgcact atctaaaaaa tcatctcccc cattgctttt cccatctcga
     4081 ttggctaata tgcaacctca atccgtagtt agaatactta atatgttaag ttttaactgt
     4141 tttttcactc taataaggat catttcaatg aataaactta ttgcctcttt ggtggcttca
     4201 agctgtgcga cggcttttgc tgctcctgtt caacagaaca atttccacac cactacccac
     4261 acttacgaat ttacgcaatc ttatgattta gttgtgccac aaggctcgaa aggaacaact
     4321 aatttgtgga ttccgttgcc gtctgatacc gattatcaaa cggtaaaatc gatagagttt
     4381 gagggaagtt acaaaaacgc ctatatcaca gaaaataacg cttatggtgc aaaaaccttg
     4441 tttgccactt gggatactga tgcaccaaag cgtgatctaa aagtgaaaat ggtgatccaa
     4501 acccaagatc gtgagcctat ggctcaaggg gcgttggcaa attaccaaat gccggagaaa
     4561 atcacttatt ctgttgatgt attggagtat ttaaaaccaa cggctcacat taaaacagac
     4621 ggtattgtga aagaatatgc cgataaaatt gtgggtaacg agcaaaatcc gttgaaaaaa
     4681 gcggaactta tccacaagtg gattgttgat aatatggaac gtgataactc ggtgctaggc
     4741 tgtggcgatg gcgatgtgga gaaaattctc accaccaaag tgctaaaagg taaatgtacc
     4801 gacatcaact cggtgtttgt agcacttgct cgtgcctcta acattcctgc tcgtgaagtg
     4861 tttggtattc gtttagggca agcggtaaaa atggggcaat attccaaaac cgcatttggt
     4921 agctcaaaag atggcgtggc gaatgaaagc ggcgggcagc actgccgtgc agagttctat
     4981 ttagcaggct tcggctgggt gcctgtagat tctgccgatg tcgctaaaat gcgtttagcc
     5041 gagaaaaaag acgtggatga tgctgatgtt caagcggtct cgaaatactt attcggcaac
     5101 tgggaaatga actgggtagg cttcaatcac ggacgtgatt tcgatttata tccacaaccg
     5161 gaacttacac cactgaataa ctttggctac ccttatgctg aggtaggtgg tgacccgtta
     5221 aactcgtttg aggcgaaaga atttggctat gaattcatct ctaaagagtt ataaccataa
     5281 atttatcaca atgtgcgtga ccgccatagt gtcggcggtg acctccacat tctgttgtat
     5341 tgggccactg gtttatttag tgtttggcat ctcttcccct tggttgatgg agctaaacca
     5401 actggagttt ctacaactgc cgatgttgct gatttcgctc gctgcgtttg gctatggctt
     5461 ctggttgctg aatttttcca acaaaatcat ctgcaccaaa tacttctccc gcagaaccct
     5521 ggtcattttg tattgggtag tttctgtggt ggtgatgttc tttttaactt acccttatgt
     5581 attgccttac attttggaaa tgtggagcgg agaataaaat gaaaaaatat atcgcaacga
     5641 tgctgttggc ttttggtttg gttcattcaa gctacgcaca agaaaaaaca gctcaaattg
     5701 aaacgaaaca agcagagaaa accgtcgtcc tccatattcc ggaaatgtac tgccagctct
     5761 gcgtttattt agtcaataaa gaactacgag cagtagaagg tgtgatttcc accaaagcct
     5821 caatgaaaga gcgaaaagcg actgtggtgg taaaagaaag tgtggataac caagccctga
     5881 tacaagcggt cggaaacctc aaatatactg caaaagtcat tgaataaaac caaagctcaa
     5941 acattattgt ttgagctttt ttaatttagc cttccttttt tcccccatct aatattcaaa
     6001 cttcactttt gttttgattt aaaatgaaaa aataatttat tttgaaagtt gtttctcatt
     6061 ttgtgatcgc tgtcacagtt tttaaaataa aaataattta ctatttgcac atctcataac
     6121 agagatgaaa gaaaaatatt ttattttgaa aaacggagat cggtatgatt gaagcaatta
     6181 cacacggagc agaatggttt atcggcctgt tccaaaaagg cggtgaaacc ttagtcggta
     6241 tgataaacgg tattttgcca ctcttaattt cattattggt tgtaatgaat gccctgatta
     6301 aatttgtagg gcaagatcgg attgaacgac ttgctcaacg tagtgccgga aaccctattt
     6361 cacgttattt gatcttaccg gtgatcggca ctttcgtttt ctgtaacccg atgacattaa
     6421 gcttagggcg tttcttaccg gagcgttaca aaccaagcta ctacgccgca gcctcttata
     6481 gttgccactc tatgaacggc ttattccctc acatcaaccc gggtgaatta ttcgtttatt
     6541 taggtattgc cagcggttta accaccttag gcttaccact tgggccttta gccatcagtt
     6601 atttattagt cggtatgttt accaatttct tccgtggctg gataacagac ttcaccaccg
     6661 cattctttga aaaacgaatg gggatcacct tagatagaga agtacattta tcccactaac
     6721 caagtaactg aaatacagag gaatagatta tgagcaacaa agcgatttac attgaaaaag
     6781 gcaacggtgg ttggggtggt ccattaacat tacctgttgt ggaaggcaaa aaagtgcttt
     6841 atatgaccgg cggcactcgc cctgcgattg ttaataaatt ggtggaatta accggctggg
     6901 aagcggttga tggttttaaa gaaggcgaac cgccggcaga agaaatcggg attgcgatta
     6961 ttgattgcgg tggggtatta cgctgtgggc tttacccaaa acgccgtatt ccaaccatta
     7021 acattcactc caccggtaaa tccggccctt tagcagaatt tattgtggaa gatatttatg
     7081 tttcagcggt gaaagaaaac aatattcgct taattgatgc cgatggaagt gtgcctgtgg
     7141 caacagagca agcaaaacca acttcatcaa cgacagcaac ggcaaaagag tatgactcca
     7201 gcaaaaaaat caccgaacaa agtgatggtt tattagcaaa aatcggtatg ggaatgggat
     7261 cggtggcggc gttattcttc caagccggac gagacactat tgatacggtg cttaaaacca
     7321 ttttaccgtt tatggcattt gtttctgcgt taatcgggat catcattgcg tcaggtttag
     7381 gtgatgtgat tgcccacggc ttgacccctc tggcaaacag cccaatcggt ttagttacac
     7441 tggcgttgat ttgttccttc cctctccttt ccccattctt agggccggga gccgtgattg
     7501 cacaagttat cggcgtgtta gtcggtacgc aaatcggttt aggcaatatt ccgccacatt
     7561 tagcactgcc ggcactcttt gctattaatg ctcaagccgc ttgtgatttt attcctgtgg
     7621 gtctttcaat ggcggaagca aaacaagata ccgtacgggt gggtgtaccg tcagtattag
     7681 tcagccgttt tttaaccggt gcaccgaccg tgctgattgc gtgggcagtt tccgcattca
     7741 tctaccaata attaaccacg atacaagcgg tggaatttga aaaatttttt gcaaaatgca
     7801 ccgcttgtca aaacgagaga acatttatat gacgattatt tatcaaacga cctttaccaa
     7861 aatcgggaat tttgctcagg aatcattaac agacgatatg ttaatcactt ttaaacaagg
     7921 cgcaccggca gatttacaag attactgctt cattcataat ccatctgaat taagtagccc
     7981 tttagaagta ggcgatatcg ctgaatttga tggcgtagct tatccgatta cggcagttgg
     8041 tagcgttgca tctgaaaact tatccgccct cggacacatt accttccgtt ttgacggggc
     8101 aaatgatgcg gaatttccgg gaagcgttca cgttatcgga acaccgccgc aaggcttaac
     8161 agagaactca accttgatta tcaagcgtga ttaatgacgg attaaaaata atttcaaatt
     8221 gaaaatttag aggaatacat tatgaacaat gaaaaaaaag ttgctatcgt tgttggcggc
     8281 ggtcaaacac ttggagcttt tttatctgaa ggcttggcag atgccggtta tcgtgtggtg
     8341 gttgctgact taaacggcga aaatgcacaa aaagtggcag ccgacattaa tgccaaacaa
     8401 ggcgaaggaa atgcgattgg ggtgcaagtc gatgcggctg atgaaccaag cgttgaagca
     8461 ctagctaaag caacagatac cgtattcggg cgtgccgatg tattggttta cagtgcaggc
     8521 acagcgaaag catcgcctat ttgggatttc aacttaaacg attttgattt gtcggtaaaa
     8581 gtgaatttaa ccggttattt cttaaccgca aaacacttct cccgcttaat gattcgtgac
     8641 ggtattaaag ggcgtatcat tcaaattaat tctaaatcgg gtaaagtagg gagtaagtat
     8701 aattccggtt atagtgcagc gaaatttggt ggtgttggtt taacacaatc gttagcatta
     8761 gacttggcag ataaaggcat taccgtgcat tcattaatgt taggcaattt attgaaatcg
     8821 ccgatgttcc aatcgttaat tccgcaatat gcggcaaaac tcggcattcc ggaaagcgaa
     8881 gtagagcaag tttatattga taaagtgcct ttaaaacgtg gctgtgatta tcaagatgtg
     8941 ttgaatgtct taaaattcta tgccggcgaa gaggcagctt attgcacagg acagtctatt
     9001 aatattaccg gtggtcaggt aatgttctaa gcaaacaagc ggtcaaattt gtcgattttt
     9061 ttacaacaat ttgcaaaaaa tgactaaaat aaaccgcttg ttaattcgcc acaccggtta
     9121 atatgccaca ggggaaagcg tattgttttg aaatcgcagg tacgtttagg ctctgtggct
     9181 ttttattaag gagcaatgat ggattcaatt acacttctga ttatattggc aatgataatg
     9241 tggctgttac aagccgtttt gggttggctt caaattaaag cctttaacca agcctttatg
     9301 gaaatcagta agaaaggcaa agtaacggtt gggcgtaata gtggtcgttt taatcctaaa
     9361 tcggttattg tgttggcgtt tgatgatgag aaaaaagtgg tagatagcct ttgtatgaaa
     9421 ggattttccg tctttgccag accgcagaaa ttagcgtctg tgattggttt gtctcttgat
     9481 caaattgtgc cggaaaaaat ctttccaagc gataaacgtt cacaatttgc cttaaaagtg
     9541 gcgatatctt ccggatatag ttgctcagat caaaaataat gcgatttttt atttcatttt
     9601 gaatattttt ttagttaaaa atgagataat ggagaataaa tatgcagatc aatagtgtca
     9661 tctttgatat ggacggggta atgattgatt cagaacccca atgggcagag gctcaaattg
     9721 cagtattagc cgacttaggt attaatatca ccgagcaaga ttgcgaacaa ctcaccagag
     9781 gtaagcggat tgatgagttg agcgacactt ggattaaaaa atttcagtta tctgtttcca
     9841 accaagcatt aagtgaagcc attttggcac acggttgtgc tgcgattgaa caacacggca
     9901 aagcattaac gggtttatat gaattattgg atttcttaaa atctaatcag atcaagttag
     9961 ccgttgcaac ctcatcgccg ccggtggtga ttaaagcggt gtttgatcgc ttaaaactgt
    10021 gggattattt cccgattcga tgcagtgcaa tggacgaaga atatggcaag ccacaccctg
    10081 cggtctattt gagtgcagtg aaaaaattag gcattgaaaa acaagagtgt attgttattg
    10141 aggacagtgt aaccggctta attgccgcca aagccgctaa tttaactaca tttttggtga
    10201 ataccgatta tcaaaatgaa aaattttcaa ttgccgataa aagaatggca tcgctactag
    10261 atgtcattga aaaattaaaa cagcattagc acagattagc tcgctccaat ctttgctaaa
    10321 ttggaggaac aatgaaacct tttgaaagaa gacagcaaat tttagcctat ttagctcagc
    10381 acggtagaac cgaagtggac gtgctggcag actattttaa gctaactggt gcaaccattc
    10441 gtaaagattt aacttcactt gaagaagata agaaagtctt gcgtacttat ggcagtgtag
    10501 tcttaattga tgaagaaatt cttgaacggg aagagaaaat caataaagag gacaaagacg
    10561 ataaaccgct tgcacagaaa accggcatta atcttcaaca aaagcaagct atcgggcaaa
    10621 aagctgcaga gttaattaat gagggaaact ctattatttt tgatgcaggc agcacagtgt
    10681 tacaaatgat tccccattta aaagcatttg ataacctctc tttaatgaca aatagtttaa
    10741 ctattttcaa tgagattttg aaactcaata aatcctataa cttactgatt tccggtggta
    10801 catttcgtga gaaatcagct tcatttcacg gttattttgc tgaatccgct tttattggtt
    10861 caaattttga catactcttt attggcacag acggactaga tttagacgtt ggattaacga
    10921 ctttcaatga agtatataaa gtcagttcgc taatgtgtga tgcggcaaat aaaatcgtct
    10981 tacttgccga ctccagtaag tttggacgta aaagtccgaa tattgtgtgt gggttagaaa
    11041 aaattcatac tatcattacc gatgaaggat tatctcaaga aatgagagaa cagattaaag
    11101 cgaaagggat tgagctgtta gttgtataag ggcgtaaaaa tgaatcattt agacagtgca
    11161 aaagaaacgt taaacctcta tgctcagcag attatcacgc taaaccatcg actttctgaa
    11221 gaatttaatg aggtggtaaa tatgatactc gcttgtcaag ggcgagtggt tgttggcggt
    11281 attggaaaat cggggctggt tggcaaaaaa atggtggcaa cttttgcctc caccggtaca
    11341 ccgagctttt tcttgcaccc gacagaggct tttcacggcg atttgggaat gctgaaaccg
    11401 attgatatgg taattctgat ttctaacagt ggtgaaactg atgatgtgaa taaactgatt
    11461 ccaagcctga aaaatttcgg caataaaatt attgcgatga caggtaatcc ttactccaca
    11521 cttgggcgaa atgccgatgt gattttaaat attggtgtag agcgtgaagc ctgcctgaat
    11581 aatctagctc caaccagctc tactttggta acgatggctt taggcgatgc gttagccatt
    11641 gcgctaatga aagcccgtga tttccgccct gaagattttg cccgctacca cccgggtggc
    11701 agtcttggtc gtaaattatt gaacagagtg cgagatgtga tggtgcgtaa agtgccaacc
    11761 gccagcttag acaccacctt taccgaatgt ttatctgtga tgaacgaagg gcgtatgggg
    11821 gtggcggtaa ttatgcaaga cgataaattg gaaggcatta ttaccgatgg tgatatccgc
    11881 cgcacattag cgaaattcgg ggcggagagc ctaaataaaa ccgctgacga aattatgacc
    11941 cgtaatccaa aaaccattaa cgataccgaa tttttagcca aagccgaaga tttaatgaaa
    12001 gagctgaaaa ttcactcatt aattgcagtg aatgatgagg gtaaagtaac gggattgatg
    12061 gagttctcta gttaatttat agatgtaacg atggcgcaag cgtttacgct tgtgccgttc
    12121 actgacatat atcaatatct aataaaacag gtaagttgtt agcataattt cttgcactgc
    12181 tatatttcca ttcaaatgcc tcgcatacaa aaccgcttac gatagggttg ttgtgaatat
    12241 ggtttagctt ttcatcaaaa aatttctgag aatagattgt tatcggttgg ttatgatgtt
    12301 gccaaagttg gtattgcttg acattatttc gttgtttagc tgcccgagta aacatccata
    12361 ataaccattc ttttctactt tcttggggat ttgttttaat ttgctgaatc aacatttag
//
LOCUS       DANLXF010000063        11930 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.063, whole genome shotgun sequence.
ACCESSION   DANLXF010000063 DANLXF010000000
VERSION     DANLXF010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11930
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.063"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            15..2681
                     /locus_tag="PXD66_001109"
     CDS             15..2681
                     /locus_tag="PXD66_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HDL6319419.1"
                     /translation="MTMEENTHTVVDAQIRQPRKISPFWLLPIVAFIIGALLFFQILK
                     EQGETITIRFSKGDGITAGKTAIRYQGLQIGQVKRVYFVDNLKEVEVQAEINPEAKSI
                     LRKQTKFWLVQPSASLAGVSGLDAIVSGNYITLLPGEGDFVDEFIAEEDPPAITVSDG
                     DLLIRLISDDIGSITVGASVYFRKVPVGNIADYRFTKDQKKIEIDVVINQKYAHLVKK
                     ESRFWNISGISADVNLQGIKINMDSLASVVQGAVAFDSPDNMEIAEQGQKFELYPDLK
                     SAKRGIEINVKLPLTHNLKINETGVFHQNVQIGMLSQLKLPQVKDNGVPQQESGQHKQ
                     IDGVLLIDPSYENLLRSQTKILLKEPKFSLNTEQLTKVGELLRGVYFEVHPGEGEPQS
                     EFVVQNEADYLLSLPNLLSFSLIAPQAYGVDTNQGIYYSDVQIGEIVKRSLELDKVRF
                     DVIIYPPYRNLIGSNSKFVAISNIDMSVGLDGLRVHAGSPSEWLKGGVRLINGKNDGS
                     PKKQYALYKDIESAESGMATLDKKATTTLSADSLSGISEGSLVLYRDFQVGEVLKITP
                     KQQKFEVELFIQPNYRHLLTEKSRFWIEPATSVDVSLKGVNITTAPLMRTLKGAISFD
                     DGGSKGNKTLYASKEKATSGNTYLTLIAKDASKLSEGMDIKYMGLTVGQIEKLELQNA
                     KKQIKATAYIQRKYYPLIAKAGSKFNVISPEISTSGFKNLDAALQNYITVDVGSGQAQ
                     NQFVLADTDTVKTTYANGFPIIVETTNANGIQPEAPVLYRGMQVGIVSRLGLSELGDR
                     VLIYVNIQDKYKHLVRTNTQFWQASGYTMDVSLQGVSMNSGTMSQLLNGGIEFSTPYT
                     KVVKPQAQPNRRFFLQRKLPDEAPAWDQGIAE"
     gene            2699..3208
                     /gene="luxS"
                     /locus_tag="PXD66_001110"
     CDS             2699..3208
                     /gene="luxS"
                     /locus_tag="PXD66_001110"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDL6319420.1"
                     /translation="MPLLDSFKVDHTKMNAPAVRVAKTMTTPKGDTITVFDLRFCRPN
                     IDILPVRGIHTMEHLFAGFMRDHLNSEQVEIIDISPMGCRTGFYMSLIGSPSAEEVVK
                     AWTASMEDALHKIPDVSAIPELNEYQCGSYKEHSLKEAHQIARNVLAAGIGINRNEDL
                     ALDEKLLNP"
     gene            3277..5124
                     /locus_tag="PXD66_001111"
     CDS             3277..5124
                     /locus_tag="PXD66_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL6319421.1"
                     /translation="MFEKLFKWFEARVETYPNEMPKTPKSGLIPFIYDATKGMRFYIL
                     LLTVLVAAVGIIEAVLFRFMGDLVDWVNKYSPTELWAEKGWTIIGMFFIALLAVVFVY
                     LASSIRFQSLQGVFPMRLRWNFHRLMLGQSLGFYQDEFAGRVSAKVMQTALSVRDVIM
                     TCADMLVYVLVYFTTSSVILLQLDGWLFVPFILWVISLAATIIYFVPKLAQAAQEQSD
                     ARSLMTGRITDAYANIATVKLFSHGNRESAYAKESMEGFMVTVHKQMRLVTVIETVTN
                     LTSMALIISTAGIGLWLWGSSVVTAGAIATSTALALRIKGLSQWIMWEFARLFENLGT
                     VQDGMITLSKPHNVVDKIDAKPLNVTKGEIKFENVDFAYDPKKPLLKDFDLTIKPGEK
                     VGLVGRSGAGKSTLTNLLLRFYDIQDGSITIDGQNIRDITQESLRSQIGLVTQDTSLL
                     HRSVRENLMYGRPNATEEEMIKAVEQAAASEFIPNLQDAKGRTGFDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEVAIQENLTQLMEGKTVVAIAHRLST
                     IMAMDRLIVLDKGEIVEQGTHEELLAQNGVYARLWAHQSGGFLPENTDI"
     gene            5311..6660
                     /gene="dnaA"
                     /locus_tag="PXD66_001112"
     CDS             5311..6660
                     /gene="dnaA"
                     /locus_tag="PXD66_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDL6319422.1"
                     /translation="MEQIVSSLWSDCLNHLQTKVSPTDYSTWLRPLQASSENGELTLY
                     AQNQFVENWVKDKFLSEITELARFLAKNDNLVVTLKVGIKPTEQPKVSPNLKNESSEF
                     SNTGFKTGLNPNQTFDNFVQGRSNQLAKLVAQQVVDNLGESHCNPLSFYAGTGLGKTH
                     LLHAIGNELVKRTPGTRVLYIHLERFYRDIIKAINSSNNDAEKIKKFYRSLDVLMIDD
                     IQFLANKPKVQEEFLHLLNALFEQRKQIILTSDVLPKNIDKIDTAIKSRLSGGISATI
                     EPPELETRVAILRQKALERGADLQEDAAYLMGQKLRTNVRELEGALNRVIAWQKLTLR
                     PITVDAVRETLQDLFASFDYLITIENIQRIVAEYYGIKVADIKSKSRARSIARPRQMA
                     MALAKELTNHSLPEIGREFGGRDHTTVMHACKTINELRDTESGIQEDYIHLTRKLSS"
     gene            6679..7782
                     /gene="dnaN"
                     /locus_tag="PXD66_001113"
     CDS             6679..7782
                     /gene="dnaN"
                     /locus_tag="PXD66_001113"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDL6319423.1"
                     /translation="MQITITREQLLKPLQQVCGVLSSRPTLPVINNLLLEIEGNRLSL
                     TGTDLEVELTTQAQLLQAVDFAGKFTIPAKKFLDISKSLPDDSVISVQFEEDKAIIKA
                     GRSKFSLATLPASDYPNLMDWKPEVDFSIDQSTLARLIDATQFSMANQDARYFLNGMK
                     FETEGNLLRTVATDGHRLAVCTMVLNQELLTHSVIVPRKAVLELSRLVASNDETVRLE
                     IGSSNIRITMNGIVFTSKLIDGRFPDYRRVLPRNADRILEAETETLKRALMRAAILSN
                     EKFRGVRLTLSNNLLKITANNPEQEEAEEIIDVAYDSPEMEVGFNVSYVLDVLNTLKC
                     QRVRFSLVDSSSSCLIEDCDNSSAEYVIMPMRL"
     gene            complement(7845..8465)
                     /locus_tag="PXD66_001114"
     CDS             complement(7845..8465)
                     /locus_tag="PXD66_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDL6319424.1"
                     /translation="MSNEYLNKTEEQQFEDAKNWFKENGTPILVAIILACAATAGWNF
                     WKNHQLETAQKTSASYQSVMESYLQNPSKNQPLVEKFIADNKDSSYAAFAQFEVAKQA
                     VEKGNFSAAKTALQSVLTYSDSTLQSIARFRLAQIDFQLKSYDEALASLAQIQDKAWE
                     LRKQILTGDILAAKGDIPAAKSAYEQAKTTANPQEQTLIDVRLNNL"
     gene            complement(8475..9746)
                     /gene="hisS"
                     /locus_tag="PXD66_001115"
     CDS             complement(8475..9746)
                     /gene="hisS"
                     /locus_tag="PXD66_001115"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDL6319425.1"
                     /translation="MAKTIQAIRGMNDCSPSESPLWQWVEQKVRNVLASYGYSEVRMP
                     IVESTPLFARAIGEVTDVVSKEMYTFWDNDEQLTLRPEGTAGCVRAAIERGWIYNNEQ
                     RLWYMGPMFRHERPQKGRYRQFHQAGVEVFGIANPEIDAELIILTARLWKELGIDQHV
                     SLQLNSIGSLEARANYRSALVKFLENHTALMNEEEKERLVKNPLRILDTKNEALQNVL
                     NDAPKLLDYLDDDSREHFTQLCALLDAMGIRYEVNPKLVRGLDYYNKTVFEWVTSALG
                     SQGTVCGGGRYDGLVEQLGGHATQGVGFAMGLERLVLLVQEVNKEIDLPKTVDVYIVF
                     SGEGATLNAFQLAEQIRSELPHVGVMTHCSGGKFQKQFKRADKVEAQIALVIGESEVA
                     EKQVVIKDLRSGAEQITIAQSELIAELSKRF"
     gene            complement(9752..10864)
                     /gene="ispG"
                     /locus_tag="PXD66_001116"
                     /gene_synonym="gcpE"
     CDS             complement(9752..10864)
                     /gene="ispG"
                     /locus_tag="PXD66_001116"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HDL6319426.1"
                     /translation="MIHQSPIKRRVSKKIYVGNVPVGGDAPITVQSMTNTRTTDVEAT
                     VAQIKSLERVGADIVRVSVPTMDAAEAFKAIKQQVSVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRTVVDCARDKNIPIRIGVNAGSLERDLQEKYGEPTPQALLES
                     ALRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARAGSV
                     KSAVGLGLLLAEGIGDTLRVSLAADPVEEVKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EIDVIATVNELEQRLEDIITPMDVSIIGCVVNGPGEALVSDLGVTGSNKMSGFYLDGV
                     RQKERFDNAKLIDQLEAKIRARVAQQHNRIDIEQIG"
     gene            complement(10965..>11930)
                     /locus_tag="PXD66_001117"
     CDS             complement(10965..>11930)
                     /locus_tag="PXD66_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HDL6319427.1"
                     /translation="TALSLGQQLKAAREALNLSITDVAQKTNLKKSHIESLENDIFIL
                     QNVAPTFVRGYVRNYVKFLRLPESLISSVNYGEVTIPKEVTKVSSVKPSSNSQGKTLK
                     YLTVLVLLAALGMTLLWWWQGYQKEQENREQFVSSAPVAEVANTATNNDASAQTSVPV
                     QQPIQVSEQPAQVAETVVAAEPALTEKVEPTQAASSPVTQEDVKVVEEVKQADATTAQ
                     VEAVNVLQQSQSEQAPTEQPVAVTNDELRIEITGSQSWITVRGAKNKRLAEKLYNNGE
                     TLSFNENEQYRLTIGAPANVKVYYKGQEVPLKIDGRVARIHLPLQ"
BASE COUNT         3462 a         2552 c         2474 g         3442 t
ORIGIN      
        1 ttccctaatt taaaatgacg atggaagaaa atacccacac tgttgtagat gcccaaatcc
       61 ggcagcctcg caaaatttct cctttttggc tgttaccaat agttgctttt attattggtg
      121 ctttgctatt tttccaaatt ttaaaagagc aaggtgaaac aatcacgatt cgatttagta
      181 aaggtgatgg cattactgcc ggaaaaaccg ctattcgtta ccaaggcttg cagattggtc
      241 aggttaagcg tgtttatttt gtcgataatt taaaagaagt ggaagtgcaa gcagaaataa
      301 accctgaagc caaaagtatt ttacgcaaac agactaaatt ttggttagtg caaccaagtg
      361 cttctttggc tggcgtttca ggcttagatg cgattgtttc tggtaactat attaccctac
      421 ttcccggtga aggcgatttc gttgatgaat ttattgcaga agaagatcct ccggctatta
      481 ccgtgagcga tggcgattta ctgatccgtt taatctctga tgacataggc tctattactg
      541 tcggagcgag tgtttatttc cgtaaagtgc cagtgggtaa tattgccgat tatcgtttta
      601 ccaaagacca gaaaaaaatc gaaattgatg tcgtcattaa tcaaaaatac gctcatttag
      661 taaaaaaaga gagccgattt tggaatatta gcggtattag cgctgatgta aatttacaag
      721 gtattaaaat caatatggac agccttgcct cggtagtaca aggtgcggtt gcttttgatt
      781 cgcctgataa tatggaaatt gccgagcaag gacagaaatt tgagctatat ccggatttga
      841 aatcagctaa acgtggaatt gaaatcaacg taaaactacc gcttacacat aatttaaaaa
      901 ttaatgagac tggtgttttt caccaaaatg tgcaaattgg tatgctttcg cagctaaaat
      961 taccgcaagt aaaagacaac ggtgtgccac agcaagaatc agggcagcat aaacagattg
     1021 atggggtttt attaattgac ccaagttatg aaaatttact acgttcccaa actaaaatct
     1081 tgctcaaaga gccgaaattc tccttaaaca cagagcagct gacgaaagta ggtgagctgc
     1141 tgcgtggtgt ctattttgag gttcacccgg gggaaggaga acctcaatcg gaatttgtgg
     1201 tacaaaacga agccgattat ctattaagtt taccgaattt acttagcttt agcttaattg
     1261 ctcctcaagc ctatggggta gataccaatc aggggattta ctatagcgat gtgcaaatcg
     1321 gagaaatcgt caaacgtagc ctagagttag acaaagtacg ttttgatgtc attatttatc
     1381 cgccttatcg caacctgatt gggagtaaca gcaaatttgt tgcgatatcc aacattgata
     1441 tgtcggtggg gctagatggg ttaagagttc acgccggttc accatctgaa tggttaaaag
     1501 gcggtgttcg cttaattaac ggtaaaaatg acggtagccc gaaaaaacaa tatgcacttt
     1561 ataaagatat agaaagtgcg gaatcaggca tggctacctt agataaaaaa gccaccacta
     1621 ccctttcggc agattcatta tctggtatca gcgaaggctc tttggtgcta tatcgtgatt
     1681 ttcaggtcgg cgaggtgtta aaaataaccc cgaaacaaca aaaatttgag gttgagctat
     1741 ttatccagcc aaattatcgt catctattaa ccgagaaaag ccgtttctgg attgagccgg
     1801 caacgagtgt tgatgtgtca ctcaaaggcg tgaatatcac tacagctcca ttaatgcgta
     1861 ccttaaaagg ggcgatcagc tttgatgatg gcggttcaaa aggcaataaa acgctttatg
     1921 caagtaagga gaaagccact tcgggcaata cttatctcac gctgattgca aaagatgcga
     1981 gtaagttaag cgaaggaatg gacattaaat atatgggctt aaccgtaggt caaattgaaa
     2041 aacttgagct acaaaatgca aaaaaacaga taaaagcgac cgcttacatt caaaggaaat
     2101 actatccgtt aattgcaaaa gccggcagta aatttaatgt tatctcgcct gaaatcagta
     2161 cctccggctt taaaaattta gatgcagcac tacaaaatta cattacggtt gatgtcggtt
     2221 cagggcaagc tcaaaaccaa tttgttcttg cagataccga tacggtaaaa accacctatg
     2281 ccaacggttt ccccattatt gtcgaaacca ccaatgctaa cggtattcag ccggaagcac
     2341 cggtgcttta ccgtggaatg caggtgggaa tagtcagccg tttagggcta agtgagttag
     2401 gcgatcgagt gcttatttat gtcaatattc aagataaata caagcactta gtcagaacca
     2461 atacccaatt ctggcaagct tcaggttaca ctatggacgt gagcctgcaa ggtgtaagta
     2521 tgaactccgg cacaatgtcg cagttattaa atggcggtat tgagttctct accccttaca
     2581 ctaaagtggt gaagcctcaa gcccagccaa acagacgatt tttcttacag cgcaaactac
     2641 ctgatgaagc cccggcttgg gatcaaggaa ttgcggaata atattgatgg agtaaaaaat
     2701 gcctttatta gatagtttta aagtagatca caccaaaatg aacgcaccag ccgtgcgtgt
     2761 tgcaaaaaca atgaccacac cgaaaggcga taccattacc gtgttcgatc tccgtttctg
     2821 tcgtccgaat attgatattc taccggtgcg tgggattcac acgatggaac atctttttgc
     2881 cggttttatg cgtgatcacc tcaatagcga acaagtggag atcattgata tttcaccaat
     2941 gggttgtcgt accggttttt atatgtcact gattggatcg ccttcagcag aagaagttgt
     3001 caaagcttgg acggcatcaa tggaagatgc gttacataaa attcctgatg tatcggcaat
     3061 ccctgagctg aatgaatatc aatgtggctc ttacaaagag cattctttaa aagaagcaca
     3121 tcaaattgcc cgcaacgtat tagctgccgg cattggtata aatcgcaacg aagatttagc
     3181 cttagacgaa aaattactta atccataatt ttttcacatt ttttcggggg caaatggcta
     3241 tttgcccctg catttttatt cggaaatcac aaatttatgt ttgaaaaact gtttaaatgg
     3301 tttgaggcaa gggtggaaac ctatccaaat gaaatgccga aaactccaaa atcaggtctc
     3361 attcctttta tttacgatgc cactaaagga atgcggtttt atatcttact actgaccgta
     3421 ttagttgcag ctgtcggtat tatcgaagcg gttctgtttc gctttatggg cgatttagtc
     3481 gattgggtca ataaatactc gccaacagaa ctttgggcag aaaaaggctg gacgattatc
     3541 ggtatgtttt ttattgccct acttgcagtg gtgtttgttt accttgccag cagcattcgc
     3601 tttcaatctc tgcaaggggt atttccaatg cgtttgcgtt ggaacttcca ccgcttaatg
     3661 cttgggcaaa gtttaggttt ctatcaagac gaatttgcag gaagagtgtc tgcaaaagta
     3721 atgcaaaccg ccctttccgt gcgtgatgtg attatgactt gtgccgatat gttggtgtat
     3781 gtgctggttt acttcaccac ctccagcgtg attttattac aacttgacgg ctggttattc
     3841 gtgccgttta ttttatgggt catctcatta gcggcaacca ttatctactt tgtcccgaaa
     3901 ttagcccaag ctgctcaaga gcaatcggat gccagaagtt taatgaccgg tcgaattacc
     3961 gatgcttacg ctaacattgc caccgtcaaa ctcttctcgc acggcaaccg tgaatctgcc
     4021 tatgcgaaag agtcgatgga aggatttatg gtgacagtgc ataaacagat gcgtttagtg
     4081 actgtaattg aaaccgtaac taatctaacc tcgatggcat taataatttc aactgcgggt
     4141 atcggtttat ggctttgggg ttcaagcgta gtaactgcag gggcgattgc cacctcaacc
     4201 gctttggctt tacgcattaa aggcttatca caatggatta tgtgggaatt tgctcgtcta
     4261 tttgaaaatt taggtacggt acaagacggt atgattacgc tttccaaacc acacaatgtg
     4321 gtagataaaa tcgatgcgaa accgttaaat gtaaccaaag gcgaaattaa atttgagaat
     4381 gtggattttg cctacgatcc gaaaaaaccg ctactgaaag attttgacct cactataaaa
     4441 ccgggggaaa aagtcggctt agtcgggcgt agtggtgcag gtaaatcaac gctcaccaat
     4501 ttattattgc gtttttacga tattcaagac ggttcaatca cgattgacgg gcaaaatatc
     4561 cgtgatatta cccaagaaag ccttcgctca caaattggtt tagtaactca agacacctcg
     4621 ctgttacacc gctctgtgcg tgaaaacttg atgtacggtc gcccaaacgc cactgaagaa
     4681 gagatgatca aagccgttga acaagctgcc gccagtgaat ttattccaaa cctgcaagat
     4741 gccaaaggca gaaccggttt tgatgcacac gttggcgaac gaggggtaaa actttccggc
     4801 ggacaacgcc aacggattgc gattgcccga gtgatgctaa aagacgctcc gattttatta
     4861 ctcgatgagg caaccagtgc gttagactct gaagtagaag tggcaattca agaaaatctc
     4921 acgcaattaa tggaaggtaa aaccgtggtt gcgattgccc accgcctctc taccattatg
     4981 gcaatggaca gacttatcgt gttagataaa ggcgaaatcg ttgaacaagg cacgcacgaa
     5041 gaattgcttg ctcaaaacgg tgtttacgcc agactttggg ctcaccaaag cggtgggttc
     5101 ttgccggaga atacggatat ttaatcaact ccatcacaca tcatactaag caagcggtca
     5161 ttttttgtta gcaatttgca agatttcaac aaaaaacgac cgcttgttcg ccaattaaaa
     5221 tcgcttccaa gcccccataa aatctctcaa aaatttgcaa gatttctccc cttttttcag
     5281 gtatcattat gacctttaat aacaataatt ttggagcaaa ttgtgtcttc cctttggtct
     5341 gattgcctta atcacttaca aacgaaagtc tcaccaactg attacagcac ttggctgcgt
     5401 cctctgcaag cgagttctga gaatggggaa cttacacttt atgcacaaaa tcaatttgtt
     5461 gaaaactggg taaaagataa atttttatcg gaaattaccg agcttgcccg ttttttggcg
     5521 aaaaatgata atttggtggt tacgttaaaa gttgggatta aaccgacaga acagccaaag
     5581 gtcagcccaa atctaaaaaa cgagagttca gaattcagca ataccggctt taaaaccggg
     5641 ctaaatccaa atcaaacctt tgataatttt gtacaaggtc gctcaaacca attagctaaa
     5701 ttagtggctc aacaagtggt ggataattta ggcgaaagcc attgcaaccc actctcgttt
     5761 tatgccggca ccggtttggg caaaacccac ttattacacg ctatcggcaa tgaattagtt
     5821 aaacgcacac cgggtactcg ggtgctatat attcatttgg aacgttttta tcgtgatatt
     5881 attaaggcga tcaacagttc caataacgat gcggaaaaaa tcaaaaaatt ctaccgctcg
     5941 cttgatgtgt taatgattga cgatattcaa tttcttgcca ataaaccgaa agtacaagaa
     6001 gagtttttac atctactcaa tgctttattt gagcagagaa agcagattat tctaacctct
     6061 gacgttttgc caaaaaatat cgataaaatt gacacagcga ttaaatcccg tttaagcggc
     6121 ggtattagtg cgaccattga gccacctgaa ttggaaaccc gagtggcaat tcttcgccaa
     6181 aaagccttag aacgtggagc agatttgcaa gaagatgccg cctatttaat ggggcaaaaa
     6241 ctccgaacca atgtgcgtga attggaggga gcattaaacc gtgtgattgc gtggcaaaaa
     6301 ttgacccttc gcccgattac ggttgatgcg gtgcgtgaaa ccttgcaaga tctgtttgcc
     6361 tcttttgatt acttgattac gattgaaaat attcaacgca ttgtggccga atattacggc
     6421 ataaaagttg ccgatattaa atcgaaaagt cgggcaagaa gcattgcccg tcctcgccaa
     6481 atggcaatgg cactggcaaa agagctaacc aaccacagct tgccggaaat tggtcgggaa
     6541 tttggcggtc gggatcatac taccgtgatg cacgcctgca aaaccattaa tgaattacgc
     6601 gataccgaaa gcggtatcca agaagattat atccatttaa cacgcaaatt atcatcttag
     6661 cccgatgaag gaatgattat gcaaattacc attaccagag aacaattact taagccttta
     6721 caacaggtgt gtggcgtatt aagtagccgt ccgactttgc ctgttattaa caatctttta
     6781 ttagaaattg aaggtaatcg tctatcttta accggtaccg atttagaagt ggaattaacc
     6841 acccaagcac aactgctaca agcggtcgat tttgccggaa aatttaccat tccagctaaa
     6901 aaattcctag atatcagtaa aagcctacct gatgacagcg tgatttcggt acaatttgaa
     6961 gaagataaag ccattatcaa agcaggacgt agtaaattta gtttagctac attaccggca
     7021 agtgattacc cgaatttgat ggattggaaa ccggaagtgg atttctcgat tgatcaatct
     7081 acccttgccc gtctgattga tgccacccaa ttctcaatgg caaaccaaga cgcccgttat
     7141 ttcttaaacg gaatgaaatt tgaaaccgaa ggcaatttat tacgcaccgt tgccacagat
     7201 gggcaccgtt tagcggtttg cacaatggtg ctaaatcaag agcttttgac tcattcggtt
     7261 atcgtgccac gcaaagccgt acttgagctt tcccgcttgg ttgccagcaa cgatgaaacc
     7321 gtgcgtttgg aaatcggttc aagcaatatc cgtattacaa tgaacggcat tgtatttacc
     7381 tctaaactga ttgatggcag attcccggat taccgccgag ttctgcctcg caatgcagat
     7441 cgcattctag aagcggaaac cgaaaccctt aaacgggcat taatgcgtgc agcgattctt
     7501 tctaatgaga aatttagagg ggtgcgttta accttaagca ataatttact caaaattacc
     7561 gcaaacaatc cggagcaaga agaggcggaa gagatcattg atgttgccta cgacagcccg
     7621 gaaatggaag tcggctttaa cgttagctat gtattagatg tgttaaacac cttaaaatgc
     7681 caacgtgtgc gttttagctt agttgattca agttctagtt gcttaattga agattgcgac
     7741 aacagctctg ccgaatatgt gattatgcca atgcgtttat aattcacagc aacactaaac
     7801 caacaaaaaa gcctgattga ttttatttca atcaggcttt gtttttataa gttattcaaa
     7861 cgtacatcaa ttaaggtttg ttcctgagga tttgcggtcg ttttagcttg ctcataagca
     7921 cttttcgccg caggaatatc gccttttgct gcaagaatat cgcctgttag aatttgctta
     7981 cgcaattccc acgctttatc ttgaatttga gccagacttg ccagtgcttc atcgtaagat
     8041 tttagttgga agtcaatttg agccaaacga aaacgagcga tagattgtaa ggttgaatcc
     8101 gaataagtca aaactgactg taacgctgtt tttgccgcag aaaaattccc tttttcgacc
     8161 gcttgtttag ctacttcaaa ttgggcaaaa gcggcatagc ttgaatcttt attatcggca
     8221 ataaatttct caacaagcgg ttgatttttg cttggatttt gcaaataact ctccatcacg
     8281 ctttggtatg aagcggatgt tttttgtgcc gtttcaagct ggtgattttt ccaaaaattc
     8341 cagccggcag ttgccgcaca agctaaaata atcgccacca aaatcggtgt cccgttctct
     8401 ttaaaccaat ttttggcatc ttcaaattgc tgctcttcgg ttttatttaa atattcgtta
     8461 ctcatctttt tctcctaaaa tcgtttagat agctcagcaa tcagctctga ttgagcaatc
     8521 gtgatttgtt ctgctcctga acgtaaatct ttgataacaa cttgcttttc agccacttcg
     8581 ctttcgccaa tcactaacgc aatttgcgct tctactttat ctgcacgctt aaattgtttt
     8641 tggaatttac cgccggaaca gtgtgtcatt acgccaacgt gtggcaattc gctacgaatc
     8701 tgctctgcaa gttggaaggc atttagtgtt gccccttcac cggagaatac gatatacaca
     8761 tccaccgttt ttggtaaatc aatctcttta ttcacctctt gtaccaacag cactaaacgc
     8821 tctaagccca ttgcaaagcc aacgccttgg gttgcgtgtc caccgagttg ttcgactaag
     8881 ccgtcataac gcccaccgcc acatacggtg ccttgtgagc ctaaggctga agtgacccat
     8941 tcaaacacgg ttttattgta atagtctaaa ccacgcacca attttggatt gacttcgtaa
     9001 cggattccca ttgcatcaag taaggcacaa agttgggtaa aatgttcacg gctgtcatca
     9061 tctaaataat cgagcagttt tggggcatcg tttaatacat tttgtagtgc ttcgtttttg
     9121 gtatctaaaa tccgtagcgg gtttttcacc aaacgctctt tctcttcttc gttcattaaa
     9181 gcggtgtgat tttctaaaaa ttttactaat gctgagcgat aatttgcacg agcttctaac
     9241 gagccgatag agttaagttg caaagaaaca tgttgatcga tgcctaactc tttccatagg
     9301 cgtgcggtga gaataatcaa ctccgcatca atttccggat ttgcaatgcc aaacacttca
     9361 acaccggctt ggtggaattg gcggtaacgc cctttctgtg gacgttcgtg gcggaacatc
     9421 ggccccatat accataagcg ttgctcatta ttgtagatcc aaccacgctc aatcgctgca
     9481 cgcacacaac cggctgtacc ttccgggcgg agcgttaatt gctcatcgtt atcccagaac
     9541 gtgtacatct ctttcgacac cacatcggtt acttcgccaa tggcacgggc aaataacgga
     9601 gtgctttcta caatcggcat acgtacttcc gaatagccgt aacttgccag cacattacgc
     9661 actttctgtt caacccattg ccataatgga gactcacttg gtgagcagtc gttcattcct
     9721 cgaattgctt gaattgtttt tgccactata tttatcctat ttgttcaata tctatacgat
     9781 tatgttgttg tgcaactctg gcacgaattt tagcttctaa ttggtcgatt aattttgcgt
     9841 tgtcaaaacg ctctttttga cgaacgccat ctaaataaaa gccgctcatt ttattgctgc
     9901 cggttacccc taaatcagac accaacgcct ctcctggacc gtttaccaca cagccgataa
     9961 tcgaaacgtc catcggcgtg ataatgtctt ctaaacgttg ttctagctcg tttaccgtcg
    10021 caatcacatc aatttcttgg cgggagcagg ttgggcaagc aataaagttg atcccacgag
    10081 agcgaatacg cagcgatttc aaaatatcaa aaccgacttt cacctcttca acaggatcag
    10141 ccgctaatga cacacgcaag gtatcaccaa tcccttccgc tagcaacaga cctaagccta
    10201 ccgccgattt caccgaaccg gctctcgctc cgcctgcttc agtaatacct aaatgcaggg
    10261 gctgatcaat ctgttttgca agaagtcggt aagattcaac cgctagaaat acatctgagg
    10321 ctttcacgct gactttgaat tgatcaaagt taaggcgatc taagtgatca acgtggcgta
    10381 atgccgattc taataatgcc tgtggtgtcg gctcgccata tttttcctgc aaatcacgct
    10441 ctaatgatcc ggcatttacc ccgattcgga tcggaatgtt tttatctctg gcacaatcta
    10501 ccaccgtgcg gatccgctct tcattcccga tattacccgg gttaatccgc aagcaatcca
    10561 ccccatattc ggcaactttc aacgcaatgc ggtaatcgaa gtggatatcc gccaccaatg
    10621 gcacactcac ttgttgttta atcgctttaa atgcctcagc agcgtccatt gtcggcacag
    10681 acacacgcac aatatcagcc ccgacccgtt ccagcgattt aatttgagca acggtggctt
    10741 ccacatcggt tgtgcgggtg ttggtcattg actgaaccgt aatcggtgca tcgccgccta
    10801 caggcacatt acccacatag attttcttcg atacccgacg tttaataggt gattgatgta
    10861 tcatattttt agctaattgt gaaaatttat aaaaacagaa aacgctcgta aagacagcta
    10921 tcgttaaatg ctagctttac gtgcgtttca tcgttaaatc aagcttactg taacggtaaa
    10981 tgaatacgag ctacacgccc atcaattttc aatggtactt cttgaccttt atagtacact
    11041 tttacattgg caggcgcacc aatggttaaa cgatattgct cattctcatt gaaacttaaa
    11101 gtctcaccat tgttatataa tttttctgct aaacgtttat ttttagcacc ccgcactgta
    11161 atccagcttt gtgaaccggt aatttcgata cgaagctcat cattcgttac cgcaacaggc
    11221 tgttctgtag gggcttgttc cgattgagat tgctgtaaaa cattaactgc ctcaacctgt
    11281 gcagtggtgg catcagcttg tttaacttct tctaccactt taacatcttc ttgtgttaca
    11341 ggtgatgatg ctgcttgtgt aggctctact ttttcagtta atgccggttc tgctgcaact
    11401 accgtttctg ctacttgagc aggctgctct gaaacctgaa taggttgctg aacaggcacg
    11461 gaagtttgtg ctgaagcatc attatttgta gcggtattcg ccacttcagc aactggtgca
    11521 gagctaacaa attgctcgcg attttcctgt tctttttgat acccttgcca ccaccaaagt
    11581 aatgtcattc ctaatgccgc aagcaatact aataccgtta aatactttaa ggttttacct
    11641 tgtgaatttg atgaaggttt tactgaagaa actttagtga cttcttttgg aatagtgact
    11701 tcaccataat taacgctcga aataagggat tcaggtaaac gcaaaaattt cacataatta
    11761 cgcacatagc cacgcacaaa agttggggca acattttgta gaataaagat atcattttca
    11821 agtgattcaa tatgtgattt ttttaagttg gttttttgag caacatctgt aatagagaga
    11881 tttaacgctt cacgagccgc ttttaattgt tgccctaaag aaagtgctgt
//
LOCUS       DANLXF010000064        11740 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.064, whole genome shotgun sequence.
ACCESSION   DANLXF010000064 DANLXF010000000
VERSION     DANLXF010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11740
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.064"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(66..1445)
                     /gene="argH"
                     /locus_tag="PXD66_001118"
     CDS             complement(66..1445)
                     /gene="argH"
                     /locus_tag="PXD66_001118"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDL6319428.1"
                     /translation="MALWGGRFTQQADAKFKYFNDSLRFDYRLAIQDIEGSIGWAKAI
                     TSVGILTVAELEQLIAALKEVRAEVESNLTIILKDDAEDIHSWVESKLIEKVGDLGKK
                     LHTGRSRNDQVAVDIKMWCKVQAVALQERIRALQERLVSVAEENQESVMPGYTHLQRA
                     QPVTFAHWCMAYYEMLERDFSRLSDANKRMSTCPLGSGALAGTAYGIDRDLLARDLGF
                     EEATRNSLDSVSDRDHILELLSTASISMMHLSRFAEDLIIFNSGESGFLEMSDRVTSG
                     SSLMPQKKNPDACELIRGKSGRVFGALNGLLTTLKGLPLAYNKDMQEDKEGIFDALET
                     WQACLEIAELVLVDIQVNTERTREAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VYAISKKEPLEALSINEFQQFHHSIGDDVYPILSLESCLEKRCAKGGVNPQRVAEAIA
                     VAKANLASK"
     gene            complement(1562..2725)
                     /gene="mnmA"
                     /locus_tag="PXD66_001119"
     CDS             complement(1562..2725)
                     /gene="mnmA"
                     /locus_tag="PXD66_001119"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDL6319429.1"
                     /translation="MTTQLTSKTYDTHFPKLTPEQLAENSKKKVIIGMSGGVDSSVSA
                     FILQQQGYQVEGLFMKNWEEDDDTDYCTAAAELADAQAVADKLGMKLHKINFAAEYWD
                     NVFEYFLAEYKAGRTPNPDILCNKEIKFKAFLEYAAEDLGADYIATGHYVRRTSDDQD
                     AKLLRGLDENKDQSYFLYALNKKQIGQSLFPVGEIEKPIVRAIAEDLGLATAKKKDST
                     GICFIGERKFKDFLARFLPAQPGEIRTVEGKLVGRHDGLMYHTLGQRKGLGIGGVKGL
                     SEDPFYVVEKDLINNVLIVAQGHDNSALLSSGLIAKQLTWVDMQPLRENLRCTVKTRY
                     RQADIACEIQVIDDETIRVMFDEPQIAVTPGQSAVFYLGEVCLGGGIIESQLK"
     gene            complement(2836..3147)
                     /locus_tag="PXD66_001120"
     CDS             complement(2836..3147)
                     /locus_tag="PXD66_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDL6319430.1"
                     /translation="MPNFGLSERATKTITDILAKYPEVEQAIIYGSRAKGNYREGSDV
                     DLTFKGKDLTYDIVKRIWGDLDDSDSPYLFDLSIYHQLTNANFIEHIDRVGKVFYQRE
                     L"
     gene            complement(3129..3545)
                     /locus_tag="PXD66_001121"
     CDS             complement(3129..3545)
                     /locus_tag="PXD66_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HDL6319431.1"
                     /translation="MNRDIRWKQRFDNYKKSVSYLQAEAEKYADTDIDVIKKGIIQSF
                     EITHELAWKLMQDILKWEGEVDIYGSKTASRLAFNRGLITQGEVWLEMIKSRNITVHT
                     DDETILTEEFNKIIKAYLPLFIEFEQRVETLCQTLD"
     gene            complement(3547..3792)
                     /gene="nqrM"
                     /locus_tag="PXD66_001122"
     CDS             complement(3547..3792)
                     /gene="nqrM"
                     /locus_tag="PXD66_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HDL6319432.1"
                     /translation="METVLLTFGFFVAVIFAMSIGFIVKGKTIKGSCGGITALGMKKM
                     CDCEEPCDNLKSKIADGTADPEEVARFNKEPQFYEVK"
     gene            complement(3805..4839)
                     /locus_tag="PXD66_001123"
     CDS             complement(3805..4839)
                     /locus_tag="PXD66_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDL6319433.1"
                     /translation="MKKFFLLLIAFFTLSACEKAPEQITLSGKTMGTTYTVKYIDNGE
                     VENLISPEKMKNELDGLLIEVNNQMSTYQQDSEISRFNQAKQANQAVEISQDFAKVVQ
                     EAIRLNKVTEGALDVTVGPLVNLWGFGPDKRLNKVPSDEQIAERSAYVGIDKILLEMS
                     SKPKLTKSAANLYLDLSSIAKGFGVDKLAEHLETIGVSNYLVEIGGELRGKGKNLQGL
                     DWRIAIEKPVMTQGQAAQITVPLHNLAMATSGNYRNYFEDEKGNRLSHIIDPKQLRPV
                     NHNLASITVFAPTTMTADGLSTGLFVLGAEKALAVAEREKLAIFLIVKNGDKFETQMS
                     SEFSKLIQSQ"
     gene            complement(4888..5181)
                     /locus_tag="PXD66_001124"
     CDS             complement(4888..5181)
                     /locus_tag="PXD66_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDL6319434.1"
                     /translation="MIDLTESQRQQVLDIFKKYLPDTPVWVFGSRIKGTAKAYSDLDL
                     ALITKQPLTIRQQTELELAFTDSDLPFTVDLIDWASCNESFKQIILQRYEVLI"
     gene            complement(5174..5608)
                     /locus_tag="PXD66_001125"
     CDS             complement(5174..5608)
                     /locus_tag="PXD66_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HDL6319435.1"
                     /translation="MEQLDITVLENAFNSLEKTIVSLENQAWFNRQEAIIQDTLIAGA
                     IQKFEFVYELSIKMLKRQLKNIESNDTDIDSTEFRDILRMAAQYGLIESPEVWLDYRK
                     MRNITSHTYDQNKAQQVYNGISNFLESTYFLVKQLQKRNSYD"
     gene            complement(5720..6949)
                     /gene="nqrF"
                     /locus_tag="PXD66_001126"
     CDS             complement(5720..6949)
                     /gene="nqrF"
                     /locus_tag="PXD66_001126"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HDL6319436.1"
                     /translation="MDSNFIFGIIAFTALVLVLAVIILFAKSKLVDSGDITISINNDP
                     EKGITLPAGGKLLGALASKGIFVSSACGGGGSCGQCKVQVKSGGGEILPTELSHISKK
                     EAKEGWRLACQVNVKSSMDVELPEEIFGVKKWECTVISNDNKATFIKELKLQIPEGEE
                     VPFRAGGYIQIEAEPHTVHYKDFDIPKEYHEDWDKFDLWRYTSKVDEHIIRAYSMASY
                     PEEKGIIMLNVRIATPPPRNPDVPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAE
                     MVFIGGGAGMAPMRSHIFDQLKRLKSKRKMSFWYGARSEREMFYVEDFDGLQAENDNF
                     KWYVALSDPLPGDREDYFRGFIHNVLYENYLKNHEAPEDCEYYMCGPPVMNAAVIGML
                     KSLGVEDENILLDDFGG"
     gene            complement(6960..7556)
                     /gene="nqrE"
                     /locus_tag="PXD66_001127"
     CDS             complement(6960..7556)
                     /gene="nqrE"
                     /locus_tag="PXD66_001127"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005529392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HDL6319437.1"
                     /translation="MEHLLSIFVKSIFIENMALSFFLGMCTFLAVSKKVSTAFGLGIA
                     VIVVLGIAVPANQLVYTHVLKEGALVEGVDLSFLNFITFIGVIAALVQILEMILDKFF
                     PALYSALGIFLPLITVNCAIFGGVSFMVQREYNFVESVVYGVGAGTGWMLAIVALAGL
                     TEKMKYSDVPAGLRGLGITFITVGLMALGFMSFSGIQL"
     gene            complement(7558..8184)
                     /locus_tag="PXD66_001128"
     CDS             complement(7558..8184)
                     /locus_tag="PXD66_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005717817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HDL6319438.1"
                     /translation="MADNNNLKKLLLSPIVDNNPIALQILGICSALAVTTQLQTAVVM
                     AIAVSLVTAFSSMFISMIRNYIPNSIRIIVQMAIIASLVILVDQILRAYAYDLSKQLS
                     VFVGLIITNCIVMGRAEAFAMKSGPVESFVDGIGNGLGYGAMLIIVAFLRELIGSGKI
                     FGITILETVQNGGWYQANGLFLLAPSAFFIIGFVIWAIRTWKPEQVEK"
     gene            complement(8184..8957)
                     /locus_tag="PXD66_001129"
     CDS             complement(8184..8957)
                     /locus_tag="PXD66_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HDL6319439.1"
                     /translation="MAKFNKDSVGGTLTVVVLLSLICSLIVAGAAVMLKPTQDIQKQL
                     DKQKNILQAAGLLQKDTNVQETYTKFIEPKIVDLATGDYVEGVTGFDARAAAKDPAQN
                     VVIAPEDDKANIKTRAKYAEVYLVKDESGKTTQVVLPMHGNGLWSIMYGFVAVQPDAN
                     TVNGITYYEQGETAGLGGEIANPNWQKNFVGKKLYNEQNQVALRVGKGVSADKDHGVD
                     GLSGATLTSNGVDGSFKYWFGQNGFGPYLAKFKAAAGAN"
     gene            complement(8950..10185)
                     /locus_tag="PXD66_001130"
     CDS             complement(8950..10185)
                     /locus_tag="PXD66_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HDL6319440.1"
                     /translation="MGLKNLFEKMEPAFHKGGKYEKWYTLFEAAYTIFYTPGTVTRKD
                     AHVRDAIDSKRMMLIVWLALFPAMFYGMYNVGAQGILAALKMGTLTDLVANNWHYSLA
                     DSLGSVADAGWGTKMLLGATYFLPIYLTVFAVGGFWEVVFAMVRKHEINEGFFVTSIL
                     LALIVPPTLPLWQAALATTFGVVVAKEVFGGVGKNFMNPALAGRAFLFFAYPGQISGD
                     AVWVAADGFSGATALSQWAVGGQGALQHVATGETITWMDAFLGNIPGSIGETSTLMLI
                     IGAAIIVFARIASWRIIAGVMIGMAATATLFNVIGSDTNPLFSMPWHWHLVLGGFAIG
                     MFFMATDPVSAAFTNKGKWAYGILIGFMCVLIRVVNPAYPEGMMLAILFANLFAPIFD
                     YLVVQGNIKRRLARVANNG"
     gene            complement(10188..11546)
                     /locus_tag="PXD66_001131"
     CDS             complement(10188..11546)
                     /locus_tag="PXD66_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HDL6319441.1"
                     /translation="MITIKKGLDLPIAGKPEQVIHNGNTVTEVAMLGEEYVGMRPSMK
                     VREGDVVKKGQVLFEDKKNPGVVFTAPASGTVTAINRGEKRVLQSVVIKVEGDEQITF
                     ARYEASQLASLTSEQVKQNLVESGLWTAFRTRPFSKVPALDAEPSSIFVNAMDTNPLA
                     ANPEVIIAEHQQAFFDGLTVLSRLFESKKVIHLCGSADSNILNAAQSRQINNVETNRF
                     KGPHPAGLSGTHIHFIDPVGATKQVWYLNYQDVIAIGKLFTTGELFTDRVVALAGPQV
                     KNPRLVRTRLGANLSQLTANELNAGENRVISGSVLSGAIATGVHDYLGRYALQVSALL
                     EGREKAFFGMIAPYSDKYSITRTTLGHFAKKLFNFTTAVHGSERAMVPTGAYERVMPL
                     DIIPTLLLRDLAAGDTDSAQALGCLELDEEDLALCTFVCSGKNNWGPLLRQALDKIEK
                     DG"
BASE COUNT         3450 a         2672 c         2103 g         3515 t
ORIGIN      
        1 aatttaatcg gcaagcggtc atatttttca atttttttgc aaaatatgac cgcttgtatc
       61 cggttttact ttgaagctaa attggctttg gctaccgcaa tcgcctctgc cacacgttgt
      121 gggttcactc cacctttggc acaacgtttt tctaagcaag attctaacga gagaattggg
      181 taaacatcat cgccaatcga atgatggaat tgctggaatt cgttaatgct taatgcttca
      241 agtggctctt ttttgctgat tgcgtaaacc accgcttcgc caacaatatg atgagcttca
      301 cggaatggca cgcctttcgc cactaaataa tctgccaact cggtcgcatt cgcataacct
      361 tgctgagcgg cttctcgggt gcgttcggta ttcacttgaa tatcaaccag tacaagttct
      421 gcaatttcta aacaagcctg ccaagtttcc aacgcatcaa aaataccctc tttgtcctct
      481 tgcatatctt tattataagc aagtggtaac cctttgagtg tagttaataa accgtttaat
      541 gccccaaata cccgaccgga tttcccacga atcagctcac aagcatcagg gtttttcttc
      601 tgtggcataa gtgatgagcc tgaggtcaca cgatccgaca tttcaaggaa gcctgattcg
      661 ccactattaa aaatgattaa atcttcagca aagcgagaga ggtgcatcat actgatcgag
      721 gcagtagaga gcagctctaa aatatggtca cgatctgaca cgctgtctaa actgttgcgt
      781 gtggcttctt caaagcctaa atcacgggct aataagtcac gatcaatgcc gtaagccgta
      841 ccagccaatg cccctgaacc aagcgggcaa gtgctcatac gtttattagc atcactcaaa
      901 cggctgaaat cccgttctag catttcataa tacgccatac accaatgagc gaaggtcaca
      961 ggctgagctc gttgtaaatg ggtatagccc ggcatcaccg actcttgatt ttcttcggca
     1021 acggaaacca aacgctcttg caaagctcgg attctttctt gcaatgccac cgcttgcacc
     1081 ttgcaccaca ttttaatatc caccgctact tggtcattac ggctacgccc ggtgtgcaat
     1141 tttttaccta aatcgcccac tttttcaatc agcttagatt caacccagct gtgaatgtct
     1201 tccgcatcat cttttaagat gatggtaagg ttagattcta cctctgctct cacctctttt
     1261 aatgctgcga ttagttgttc taattctgct accgttaaaa tacctaccga agtaatggct
     1321 tttgcccagc cgattgagcc ttcaatatct tgaatcgcca agcggtaatc aaaacgtaat
     1381 gaatcgttaa aatatttaaa ttttgcgtct gcttgttgtg taaagcgtcc accccaaagt
     1441 gccacagtct gttccttgta tattattctt tgtatcgatc aaaatgaaaa aggcgtttta
     1501 ccgccttaac ttttcgctta ttttgcataa atatgcaaaa tatttcaata tttatttata
     1561 actattttaa ttgtgactca ataatgccac cgcctaagca aacttcacct aagtaaaaca
     1621 cggcagactg ccccggcgtc acggcaattt gcggttcatc aaacatcact cgaatggttt
     1681 catcatcaat cacttggatt tcacacgcaa tatctgcttg acgataacgg gtttttaccg
     1741 tgcagcgtaa attttcacgc aacggctgca tatccaccca agtgagctgt ttcgcaatta
     1801 agccacttga aagcagggct gaattatcgt gtccttgtgc cacaatcaat acattgttaa
     1861 ttaagtcttt ctccaccaca taaaacggat cttcacttag ccctttcacc ccaccgatac
     1921 ccaagccttt gcgttgccca agcgtgtgat acatcagccc atcatggcga ccgactaatt
     1981 tgccctcaac ggtgcggatt tcccccggtt gagccggtag aaaacgagct aagaaatctt
     2041 taaatttacg ctcaccgata aaacaaatgc cggtagaatc ttttttcttt gcggttgcta
     2101 atcctaggtc ttcggcaatg gctcgcacaa tcggcttctc aatttcaccc actgggaata
     2161 aactctgccc gatctgcttt ttatttaacg catataaaaa gtaactctga tctttgtttt
     2221 catctaagcc acgcaaaagt ttcgcatctt gatcatcgct cgtacggcgg acgtaatgcc
     2281 cggtcgcaat ataatccgca ccaagatctt ctgccgcata ttccaaaaag gctttaaatt
     2341 tgatctcttt attacacaaa atatccggat ttggcgttct tcctgccttg tattctgcca
     2401 agaaatattc aaatacatta tcccaatatt ccgctgcaaa attaatttta tgcagtttca
     2461 tacccagttt atcggcaacc gcttgagcat cggcaagctc ggcggcggcg gtacaataat
     2521 ccgtatcatc atcttcttcc cagtttttca taaacaagcc ttccacttgg tagccctgct
     2581 gttgaagaat aaaagcggaa accgaggaat ccaccccacc ggacataccg ataatgactt
     2641 ttttcttgct gttttcagca agttgttctg gggttaattt agggaaatga gtgtcgtagg
     2701 tttttgaggt taattgagtt gtcataaatc tcccgtaact tcggttaagg cgaagcacat
     2761 tggttaataa taaaaacaac aagcggtgaa atttgcaaaa aattttgcaa aatgaaccgc
     2821 ttgcctatta agaaattaga gttctctttg gtaaaagact tttcctactc tatcaatatg
     2881 ttcaataaag ttggcattgg ttaattgatg ataaatcgac aagtcaaata aatagggcga
     2941 gtcggaatcg tctaaatcac cccaaatgcg tttgacaata tcgtaagtaa gatcttttcc
     3001 tttaaaagta agatctacat ctgagccttc acgataatta cctttagcac gcgagccgta
     3061 aataatggct tgctccactt cagggtattt cgcaagaata tcggtaatag ttttcgtcgc
     3121 tcgttcgctt agtccaaagt ttggcatagt gtttctactc gctgttcaaa ttcaataaat
     3181 aaaggcagat acgccttgat aattttatta aattcttcag ttaaaatagt ttcatcgtca
     3241 gtatgaactg ttatattgcg gctttttatc atttctagcc aaacttcgcc ctgagtgatt
     3301 agcccacgat taaaagccaa acgagaagcc gtttttgaac cataaatatc aacttcacct
     3361 tcccatttta aaatatcttg cattaacttc cacgctaact cgtgtgtaat ctcaaaactc
     3421 tggataattc cttttttaat tacatcaatg tcggtatcag cgtatttttc tgcttctgct
     3481 tgaaggtaac ttactgattt tttatagtta tcaaaccgct gtttccagcg aatatctcta
     3541 ttcatattat ttcacttcat aaaattgtgg ctctttatta aaacgtgcca cttcttcagg
     3601 gtcggctgtg ccgtctgcga ttttagattt taggttatcg caaggttctt cgcagtcgca
     3661 catttttttc atacctaaag cggtgatacc gccacagctg cctttgatgg ttttgccttt
     3721 cacgataaaa ccaatcgaca tcgcaaaaat tacggctaca aaaaagccaa aggttaataa
     3781 tacagtttcc atttttcact ccaactattg tgattgaata agtttgctaa attcgctcga
     3841 catttgtgtc tcaaatttat cgccgttttt aacaattaaa aaaatagcta atttttcacg
     3901 ctctgccact gctaatgctt tttccgctcc taaaacaaat aaaccggttg ataagccgtc
     3961 tgccgtcatt gtagttgggg caaatacggt gatagaggct aaattatggt ttaccggacg
     4021 taactgtttt gggtcgataa tatgtgaaag acggttgcct ttttcatctt caaaataatt
     4081 acgataattg cccgaagttg ccatcgccaa attgtgcaac ggcacggtaa tttgtgcggc
     4141 ttgaccttgt gtcattaccg gcttctcgat tgcaatccgc caatctaagc cttgtaaatt
     4201 ttttcctttg cctcgtaatt cgccaccaat ttcaactaaa tagttgctca cacctatagt
     4261 ttcaagatgc tcggctaatt tatctacacc gaaacccttt gcaatagacg ataaatctag
     4321 ataaagatta gctgctgact tggtgagctt gggtttacta ctcatttcaa gcaaaatttt
     4381 atcaatgccg acataagcag aacgctctgc aatttgttca tcagacggca ctttatttaa
     4441 gcgtttatcc ggcccgaagc cccacaagtt caccaatgga ccaacggtta catctaaagc
     4501 cccttcggtt actttattta aacggattgc ctcttgtacc acttttgcaa aatcctgcga
     4561 aatttcgacc gcttgatttg cctgctttgc ttgattaaaa cggctaattt ccgaatcttg
     4621 ttggtaggtg gacatctgat tattcacctc aatcaataag ccatccagct catttttcat
     4681 tttttcaggc gaaatcaaat tctccacttc accattatca atatatttta cggtgtaagt
     4741 cgttcccatg gttttgcctg acagcgtaat ttgctcaggg gctttttcac aagcacttaa
     4801 agtaaaaaaa gcaattaaaa gaagaaaaaa ttttttcatt ggtgtcacaa aagttaaaaa
     4861 ttggggaaat tatagcatat ttagcgatta aataagcact tcatatcgct gtaaaataat
     4921 ttgtttaaac gattcgttgc aacttgccca gtcaatcaga tctactgtga aaggtaggtc
     4981 agaatccgta aacgcaagct ctaactctgt ttgttgccga attgtgagcg gttgtttggt
     5041 gattaatgct aaatcaagat ccgaataggc tttggctgtg cctttaatgc gagagccaaa
     5101 aacccaaacg ggtgtatcag gcaagtattt tttgaaaata tccaacacct gttggcgttg
     5161 tgactcagtt aaatcaatca tagctattgc gtttttggag ctgtttaact aaaaaatacg
     5221 tactttccag aaagtttgaa attccgttat aaacctgctg tgctttattt tgatcataag
     5281 tatgcgaagt aatatttcgc attttgcgat aatccagcca aacttccggt gattcaatca
     5341 gcccatattg agcagccatt cgcaaaatat cacggaactc ggtgctatca atatcagtgt
     5401 catttgactc aatgtttttg agctgacgct tgagcatttt tatcgacaat tcgtaaacaa
     5461 attcaaattt ttggattgct cctgcaatca aagtgtcttg aataattgct tcttgccggt
     5521 taaaccacgc ttgattctca aggctgacaa tcgttttctc aagggaattg aaggcatttt
     5581 caagtaccgt aatatccagt tgttccattt gcttcccctt aaaattcaca ttattaaatt
     5641 accaaaactt aataatataa attaaatcaa gcggtcaatt ttgcaaattt gaccgcttgt
     5701 agtcagaagt taaggtttat caaccaccaa agtcatctaa taagatgttt tcgtcttcta
     5761 cacctaaact cttcagcata ccgattaccg cagcattcat caccggtgga ccacacatat
     5821 agtattcgca gtcttcaggt gcttcgtggt ttttgaggta gttctcataa agcacgttat
     5881 gaataaagcc acggaagtaa tcttctctgt cgcccggtaa tggatcagaa agtgccacat
     5941 accatttgaa gttatcgttt tctgcttgaa gaccatcaaa atcttccaca tagaacattt
     6001 cacgctcaga acgagcacca taccagaatg acattttacg tttagatttt aaacgtttta
     6061 attggtcgaa gatatgtgaa cgcattggtg ccatacctgc accaccaccg ataaagacca
     6121 tttccgcatc ggtatcttta gcgaagaact caccgaatgg acctgaaatt gttactttat
     6181 cacccggttt taatgaccaa atgtaggaag acatttgacc cggcggtacg tccggattgc
     6241 gtggtggagg tgtagcaata cgcacgttaa gcataataat gcctttctct tccggatatg
     6301 aagccattga gtatgcacga ataatatgct cgtccacttt tgaagtataa cgccataagt
     6361 caaatttatc ccagtcttcg tggtactctt ttggaatatc gaagtcttta taatgaacgg
     6421 tatgaggttc agcttcaatt tggatataac cacccgcacg gaacggtact tcttcgcctt
     6481 caggaatttg aagtttaagc tctttgatga aggttgcttt gttatcgttt gagataacgg
     6541 tacattccca ttttttcaca ccaaagatct cttccggaag ttcaacgtcc attgaagatt
     6601 tcacgtttac ctgacaagct aaacgccagc cttctttcgc ttctttctta gaaatgtgcg
     6661 ataactcagt tggaagaatt tcaccaccgc ctgatttcac ttgtacttta cattgaccgc
     6721 aagagccacc gcccccacaa gctgacgaaa cgaaaatccc tttacttgct aaagcaccga
     6781 gtaattttcc accagccggt agcgtaatgc ctttttccgg atcgttgttg attgagatgg
     6841 taatatcacc tgaatcaact aattttgatt tagcgaaaag aatgatcacc gcaagcacta
     6901 ataccagagc cgtaaatgcg ataataccga aaataaaatt actatccacg atatgctcct
     6961 tataattgaa tgcctgagaa tgacataaag cctaacgcca ttaagcctac cgtaataaag
     7021 gtaataccta aaccacgtaa gcctgccggc acgtcggaat atttcatttt ctcggttaaa
     7081 cctgcaaggg caacgatggc taacatccag cctgtacctg cacctacgcc ataaaccaca
     7141 gattctacaa agttatactc acgttgtacc ataaacgata caccaccaaa aatcgcacag
     7201 tttacggtga taagtggtaa gaagatacct aatgcactat aaagtgccgg gaaaaacttg
     7261 tctaaaatca tttccagaat ttgaacaagt gccgcaataa ccccgatgaa agtgataaag
     7321 tttaagaagc ttaaatccac accttctact aatgcaccct cttttaatac gtgggtataa
     7381 actaattggt tagctggaac tgcaatgcca agtactacaa ttaccgcaat acctaaacca
     7441 aaagcagttg aaactttctt agacaccgca aggaatgtac acataccaag gaagaaagaa
     7501 agtgccatat tttcaataaa tatggactta acaaagatac tcagaagatg ttccatctta
     7561 tttctccact tgttccggtt tccacgttct aatcgcccag atcacaaatc cgataataaa
     7621 gaacgcactt ggtgctaata agaataaacc gtttgcttga taccaaccgc cattttgaac
     7681 agtttcaaga atggtgatac cgaaaatttt acctgaaccg ataagttcac gtaaaaaggc
     7741 aacgataatt aacattgcac cgtagcctaa accgttaccg ataccatcaa caaaactttc
     7801 aaccgggcct gatttcatcg cgaatgcttc tgcacgcccc attacgatac agttggtaat
     7861 aataaggcct acgaataccg ataattgttt tgacagatcg taagcataag cgcgtaagat
     7921 ttggtcaacc aaaattacta atgatgcaat aatcgccatt tgcacaatga tacggatact
     7981 atttggaata tagttacgaa tcattgaaat gaacatactt gagaatgcag taactaaact
     8041 taccgcaatc gccattacta ctgctgtttg taattgagtc gttaccgcta acgcagaaca
     8101 aatacctaag atctgtaatg cgatcgggtt gttatcgaca attggcgata acaataattt
     8161 tttaagattg ttattgtctg ccattagtta gctcctgccg ctgctttaaa ttttgctaaa
     8221 tatggaccaa aaccattttg accaaaccag tatttgaatg aaccatcaac accgttcgag
     8281 gttaatgttg caccagataa accatcaaca ccgtgatctt tatctgcaga taccccttta
     8341 cccactctta acgcaacttg gttttgctcg ttatacagtt ttttacctac aaagtttttc
     8401 tgccagttcg ggtttgcaat ttcgccacct aagcctgccg tttcaccttg ttcatagtaa
     8461 gtaataccgt taacggtatt tgcatcaggt tgtaccgcaa cgaaaccgta cataattgac
     8521 cataagccgt taccgtgcat tggtaatact acttgagtcg ttttgccaga ttcatctttt
     8581 actaaataaa cttcagcata tttagcacgc gttttgatat tcgccttatc atcttcaggt
     8641 gcgataacta cattttgagc aggatcttta gctgctgcac gagcatcaaa accggttaca
     8701 ccctcaacgt aatcacctgt tgctaaatcg acaattttcg gctcaataaa tttggtatag
     8761 gtttcttgca cattggtatc tttttgcaat aaaccggccg cttgtaagat gtttttctgt
     8821 ttatcaagtt gtttctgaat atcttgtgtt ggtttaagca ttactgccgc acctgccaca
     8881 atcaaagaac aaattaaact taataaaact acaacggtta gcgttccgcc aacgctatct
     8941 ttattaaatt tagccattgt ttgctaccct cgctaatctg cgtttgatat tcccttgaac
     9001 tactaaatag tcaaagattg gagcaaataa gttagcaaat aagatcgcta acatcatacc
     9061 ttcagggtat gctgggttta ccacacggat taatacacac ataaagccga ttaagatacc
     9121 gtatgcccat ttacctttgt tggtaaatgc agctgatacc gggtcggttg ccataaagaa
     9181 cataccgatc gcaaaaccgc ctaatactaa atgccaatgc caaggcatag agaataatgg
     9241 gttggtatca gaaccgatta cattaaataa tgtcgcagtt gccgccatac caatcattac
     9301 accggcaata attcgccaag aagcaattcg ggcaaataca atgattgcag caccaataat
     9361 caacattaag gtagaggtct cgccgataga acccggaata ttacctaaga acgcatccat
     9421 ccaagtaatg gtttcaccgg ttgcaacgtg ctgtaatgca ccttgaccac caactgccca
     9481 ttgagaaagt gcagttgcac ctgagaagcc gtctgcggca acccatactg catcacctga
     9541 aatttgaccc gggtaagcaa agaacaagaa agcacgaccg gctaacgcag ggttcataaa
     9601 gtttttaccc acgccaccga acacttcttt tgcgaccaca acaccaaagg tagttgcaag
     9661 ggctgcttgc cataacggta atgttggagg aacaattaat gctagtaaga tagaggttac
     9721 aaagaaacct tcgttgatct cgtgcttacg caccattgca aacaccactt cccagaaacc
     9781 gcccactgca aatactgtta agtagattgg taggaaatag gttgccccca gcaacatttt
     9841 agttccccag cctgcatcag ctacagagcc taaactatcg gctaagctgt agtgccagtt
     9901 gttagcaact aaatccgtta atgttcccat tttgagtgcc gctagaatac cctgtgcacc
     9961 aacattgtac attccgtaga acatcgcagg gaataacgct aaccatacaa ttaacatcat
    10021 acgcttagag tcaatcgcat cacgcacgtg agcatcttta cgagtgaccg tacccggtgt
    10081 atagaaaatc gtataggcag cttcaaatag cgtgtaccat ttctcatact ttccaccttt
    10141 atggaaagcg ggttccattt tttcaaaaag atttttcaaa cccattttta accgtccttc
    10201 tcgatcttat ctaacgcttg acgtaataat ggtccccagt tgtttttacc ggaacacaca
    10261 aaagtacata acgctaaatc ttcttcgtct aactctaagc agcctaaagc ttgagcggag
    10321 tctgtatctc cagctgctaa atcacgcaat aagagtgttg gaatgatatc caacggcatt
    10381 acacgctcat aagcccctgt tggtaccatt gcacgctcac taccgtgaac tgctgttgtg
    10441 aagttaaata atttcttcgc aaaatgccct aaagtagtac gcgtaattga atacttatca
    10501 gagtaaggag caatcatacc gaagaacgct ttctcacgcc cttctaataa agcagacact
    10561 tgtaatgcgt aacgacctaa atagtcgtgt actcccgttg cgatagcacc acttaacaca
    10621 gaacctgaaa tgacacggtt ttcacccgca tttaattcgt tggctgttaa ttgtgaaaga
    10681 ttcgccccta aacgagtacg caccaaacgt ggatttttca cttgtggccc ggctaatgcg
    10741 actacacggt cagtaaatag ctcaccggtc gtaaataatt taccgatagc aattacatct
    10801 tggtaattta aataccaaac ttgtttagtt gcacctaccg ggtcaataaa gtgaatatgc
    10861 gtaccagaaa gacctgcagg gtgcggacct ttaaagcggt tagtttccac attattgatc
    10921 tgacggcttt gtgccgcatt taaaatattt gagtctgcac tgccacagag atgaatcact
    10981 tttttacttt caaataaacg acttaataca gttaagccat caaagaaagc ttgttgatgt
    11041 tctgcaataa tcacttccgg atttgctgca agtggattgg tatccatcgc attgacaaaa
    11101 atagaggaag gttctgcatc taatgcaggc actttgctaa acggacgtgt acggaaagcc
    11161 gtccataagc ctgactctac aaggttttgt tttacttgct ctgaagttaa tgaagctaat
    11221 tggctggctt cataacgagc aaaggtaatt tgctcatcac cctcaacttt aattactacc
    11281 gattgaagaa cacgcttctc gccacggtta attgccgtta cggtaccact tgcaggggca
    11341 gtaaatacca cgcccggatt ctttttatct tcaaaaagaa cctgaccttt ctttactaca
    11401 tcgccttcac gcactttcat tgaaggacgc atacccacat attcttcacc aagcatagca
    11461 acttcagtaa cggtattacc gttatggatt acttgctcag gttttcccgc aatcggaaga
    11521 tctaagcctt ttttgattgt aatcatattg tttgcactac ttttcttttg ggttaaaaaa
    11581 tatgattgcg ctaacgcaat cgcttctatt gagccgcagc tttacttaaa aataaacaat
    11641 ggtcccccac ctatttttaa aagccacatt aagttctcaa atctgtgatc aacctttatc
    11701 ttttcttaac aaaaggttaa ctaaaaataa tcttcttatt
//
LOCUS       DANLXF010000065        11634 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.065, whole genome shotgun sequence.
ACCESSION   DANLXF010000065 DANLXF010000000
VERSION     DANLXF010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11634
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.065"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            60..1568
                     /locus_tag="PXD66_001132"
     CDS             60..1568
                     /locus_tag="PXD66_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HDL6319442.1"
                     /translation="MSNQSSNAPQRETFSGRGAFIMAAIGSAVGLGNIWRFPYTTYEN
                     GGGAFILPYIIALLTAGLPLLFLDYSIGHKYRGGAPLAFRRFSSKFETFGWWQVLINV
                     IIGIYYAVVLGWAASYTYFSFSGAWGDKPVDFFIGEFLKMGDITQGVSFEFVGMVTGP
                     LIAVWLIAIAVLAMGIKKGISAASNLLMPQLIVMFLVLVGYSLTLPGAAKGLDALFTP
                     NWEKLADPSVWIAAYGQIFFSLSICFGIMITYASYLKKETDLTGSGLVVGFANSGFEL
                     LAGIGVFAALGFMATAAGQEVNEVAKGGIGLAFFAFPTIINQAPMGALVGVLFFGSLT
                     FAALTSFISVIEVIISAVQDKLNSGRVKATLLVGIPMMVVSVLLFGTTTGLPMLDVMD
                     KFVNQFGIVAVAFVSLIAIVTSEKLSTLEKHLNEVSSFKVGFIWRLCIVITSGVLAFM
                     LFSEIAKVYREGYEGYPSWFVNTFGWGMAIGLVVVAYLLSKMAWKNEPKGDE"
     gene            1568..1666
                     /locus_tag="PXD66_001133"
     CDS             1568..1666
                     /locus_tag="PXD66_001133"
                     /inference="COORDINATES: protein motif:HMM:NF028261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HDL6319443.1"
                     /translation="MSSAAIITMVVAMTIIWGGLLFAIKRLPKEEQ"
     gene            complement(1714..1977)
                     /locus_tag="PXD66_001134"
     CDS             complement(1714..1977)
                     /locus_tag="PXD66_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrxA family glutaredoxin"
                     /protein_id="HDL6319444.1"
                     /translation="MFVEIYGRLSCPYCVRAKTLAEKMKQELSDFDFKFIDMIAENIS
                     KQDLEPRVGKPVATVPQIFVDNNHVGGCTDFQAFVEEKFGITV"
     gene            2137..3471
                     /gene="srmB"
                     /locus_tag="PXD66_001135"
     CDS             2137..3471
                     /gene="srmB"
                     /locus_tag="PXD66_001135"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HDL6319445.1"
                     /translation="MTQLMTFEELDLSPQLLKALAHKGYNRPTAIQQATIPAALEGRD
                     LLGSAPTGTGKTAAFLLPAIQHLLDYPRRKPGAPRILILTPTRELAMQVAEQAQALAK
                     FTQLSIATITGGVAYQNHGEVFNKNQDIVVATPGRLMQYIKEENFDCRAVEVLIFDEA
                     DRMLQMGFGQDAEKIAAETRWRKHTWLFSATLEGELLEDFAERLLNDPIKVDAEPSRR
                     ERKKIQQWYYHADNVEHKTKLLARIISEFSVERAIVFVRRREDVRELSETLRKRGIRS
                     TYLEGEMAQTQRNQAINRLKEGVVNVLVATDVAARGIDIDDIDFVLNYDLPYSADTYL
                     HRIGRTARAGKKGSAISLVEAHDYKLLGKIKRYTEEVLKPRIIEGLEPRTKAPKDGEL
                     NVTSKKEKARIKKKKEAKKEAAKPKVKVRHKDTKNIGKRRKPSSESAAVKQA"
     gene            complement(3540..3833)
                     /locus_tag="PXD66_001136"
     CDS             complement(3540..3833)
                     /locus_tag="PXD66_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HDL6319446.1"
                     /translation="MSKTMQQKAAEAHNMCRLKGDSMLDNADRQIAFEAVYESEELAQ
                     QAVEFFAQKAKSVETEPCEIQSEITQIAEGFLMKMWITFSCQAEVILFQMAVR"
     gene            complement(3835..4497)
                     /locus_tag="PXD66_001137"
     CDS             complement(3835..4497)
                     /locus_tag="PXD66_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDL6319447.1"
                     /translation="MPFPDEISELKGYFDGTNPVSAYPAQGLNVPFYILGSSPESAYL
                     AAERGLPYAFASHFAPRFMDDAIRIYRSHFKPSDVLDKPYVILGVNAIVAETDEEATQ
                     LATTQTQFFLNVVTNAQQNLQPPLESDDKVWENFVKAEIVPHFGPVAFEQNALYRQEK
                     TVVRQMTECSLIGSPQSVEQQLKALKARVEIDEIMAVNYIFDEQKQHQSYTWLKQITD
                     KV"
     gene            complement(4481..4933)
                     /locus_tag="PXD66_001138"
     CDS             complement(4481..4933)
                     /locus_tag="PXD66_001138"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MsnO8 family LLM class oxidoreductase"
                     /protein_id="HDL6319448.1"
                     /translation="MVLQVLNSKAIQPFLYANSENKMTNLSILNLVPIREGQTAKQAV
                     DSMVKLAKHAENIGIERYWIAEHHNMKNLASSATQLLIQHALSHTEKLRVGSGGVMLP
                     NHSPYIVAEQYGTLETLYPNRVELGLGRAPGTDMRTASALRPEFAFPR"
     gene            complement(4953..5591)
                     /locus_tag="PXD66_001139"
     CDS             complement(4953..5591)
                     /locus_tag="PXD66_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL6319449.1"
                     /translation="MKKFALKTVLVALSALFAFNATAVAADPTAGKEYIEVRKAPSAQ
                     KEVVEFFSFYCPHCYDFELNYKIPSQIKAKLPADSKLVQYHVNFLGRQSENLTRAWAL
                     AMALGAEDKVKTALFEAAQKDAFKSMDDIRAVFIANSISAEQFDSGINSFAVNGLVNK
                     QVQLAEDFQIRGVPAFFVNGQYQLNLEGFSDSSSTNDFIQRYIDAVVFLSKK"
     gene            complement(5635..5904)
                     /locus_tag="PXD66_001140"
     CDS             complement(5635..5904)
                     /locus_tag="PXD66_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HDL6319450.1"
                     /translation="MKCKRLEELLELLGEHWRKNPDLHLIDILQQLSVEVSEPDNFHA
                     LRDEVLIYQLKMRNADKFEPIPGIKKDYEDDFKTALLRARGILND"
     gene            5996..7105
                     /gene="mobA"
                     /locus_tag="PXD66_001141"
     CDS             5996..7105
                     /gene="mobA"
                     /locus_tag="PXD66_001141"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HDL6319451.1"
                     /translation="MEEKISAVILAGGLARRMNGAEKGLQLLDQKPLISHLLARLSPQ
                     IEDIWLNINRSVEQYQQLYPELPYYQDSLPDFQGPLSGMLSGFEQIESDYLLFVPCDT
                     PFIPTQLLHKLHTALRINQAQIAYAHDGDRPHPTVALVHRSVAENLRAYLASGERRLL
                     WFFQTQKSVAVDFSAYKAAFRNFNCLEDLTAFNPYPIKTLAITGYSGTGKTTLLEKLI
                     PKLTACGIRVGLIKHSHHNVDIDKKGKDSYRLREAGANPTMVVCDERWALMTETKQAA
                     ETKQAVEFSQLIAKFDPETVDLVLVEGFKHETMPKIQLHRKEIEKPLPALDQWTIATA
                     TDYPLERENRLDINDVEQLADFIRQWLEASKEPAN"
     gene            complement(7160..>11634)
                     /locus_tag="PXD66_001142"
     CDS             complement(7160..>11634)
                     /locus_tag="PXD66_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDL6319452.1"
                     /translation="FVAFAVSLSATASVFRDDIDLQYYRDFAENKGKFTVGASNIEIK
                     DKSGNTLDALFPNVPMPDFSAANRNLGIATLVAPQYLVSVAHNTQYNTVEFGAPGTNA
                     DAHHYTYKVVDRNDYGIVEGGQHQDYQVPRLNKLVTEVAPATVTDLGNNASAYQDSSR
                     FTHFARLGSGRQIVKNTEHKDNQISTSYQYLTGGVHLPIAVHNSDYWLDFRGNALNNS
                     PYGALTAFGTRGDSGSGVYGYDQKTKRWLLLATYTFGTPANNYYNRAGIIRQDYHDKQ
                     FAEDIAGTLTNAQQNAVFEWSAQGKDSSIGNKGKNLTVSLADRSIKDNSASPLGYGGT
                     PQLQLPQDNTGKTLNIEGQDSTIVLKTDIDQGAGALNFNANATVRPENDQSWVGAGIV
                     VAKGKQVNWQVKNPQGDRLSKLGEGTLHINGKGENLGDISVGQGTVILTQQAGENGKK
                     SAFNQVGIVSGRSTVVLNSADQVDPNKIYFGFRGGRLDLNGNNIAFNRIQNSDDGARI
                     VNNHLQKAATLTINGPKPPEATDLKWGTWKENSADIYEYINPHANNRTDYFTLKGNPN
                     QYMPTNGASNAHWTFLSSNKDAAVKQVLAQKGLEHRYNSFNGFIGETDNTQHNGRLNV
                     VYDPKASTPPATASEVTWGKGLVAGADIYLFTNPKTKIREYFALKGDPKQPVPRGGLS
                     SEHWEFLAADRNQAINKVLARKNAPIEQEKLNSIYTFSGGLNLNGDLTVKGGKVLLSG
                     RPTPHAYDAINKQDVVYADDWQNRQFKADNMQLNQYAQLYVGRNVSNLQANLSANDHA
                     QLHLGFINEQTPSCYYSEYTGKTSCDTQAVVSNEIFATLPTTQINGDVSLAEQSQLHI
                     GKANLIGTIQAAATTSIRLANQASWTNTGDSRTGNLVAENGSTINLNEKFVTGEIPTR
                     FNTLIIDGNFQGNAKINYLTDIAAGKGDHLQVNGLAEGTFTLALRNSGKEAEVVSPLS
                     LLTLTHQAQADKAKVSLENGYVDLGAYRYVLANRSNGYRLYNPLKDAKDRNQSIATAK
                     AELDHAIAEADKQKQEISRLNAEAEKERQAEKNAKQAAANAQSQLSQANSELTRLQQY
                     ADYYRRYYPTYYRQIQGQITVAKQKVTQASAALTTAENNAKASAAQIANVEQAVETAQ
                     NIAKQVEAKLATLRITAGNSEAILNAEALKLCQENGANCEHLQAHADTDSSNIQQSDW
                     VSQYANTALSELSAQANSALQIGQDLDRQLFAKHDKFHVWSSIEHQKTEHQSDLYRPY
                     EQQTNLTQLGIELPLANGINAGVMLSRNHANAEFDEGVNGKSNLLMASLYGKWHSENG
                     TFVSLDGSYGKAKNRIDLFGENRFNRHIMAIGANLGHNFDLAGVQVQPAVGTRYYRFS
                     AQNYKLGEVEVRSPKAHFMAYQAGVKVSKAFDLSSWKVEPSLAAHYVDASSKRLSVAV
                     NDNTFSQRFGRYLKTEVGVGVTKGQWQLSSHLGLLKGNEIGKQHFAGFKLGYSW"
BASE COUNT         3056 a         2442 c         2627 g         3509 t
ORIGIN      
        1 aatttacatt taactcaatt cttttttata taaaacttta tcagtttgag gtatttaccg
       61 tgtctaatca atcatcaaac gcaccgcaaa gagaaacgtt ctccggacgt ggtgcattta
      121 ttatggcggc tattggttcc gctgtcggct tagggaacat ctggcgtttc ccttatacta
      181 cttatgaaaa cggcggtggg gcttttattc tgccgtatat cattgccctt ttgaccgcag
      241 gtttaccgct gctgttctta gattattcta tcgggcataa atatcgtggt ggtgcaccgc
      301 tcgctttccg ccgtttcagc tcgaaatttg aaaccttcgg ttggtggcaa gtgctgatca
      361 acgtgatcat cgggatttat tatgcggtgg tactcggctg ggcagcaagt tacacctact
      421 tctcattcag cggagcgtgg ggcgataaac cggtggattt ctttatcggt gaattcttaa
      481 aaatgggcga tattactcaa ggcgtgagct ttgaatttgt cggtatggtt acagggccgt
      541 taattgcggt atggctgatt gctattgctg tgttggctat ggggatcaaa aaagggattt
      601 ctgctgcctc aaacctgcta atgccgcagt tgatcgtgat gttcttagtg ttggtcggct
      661 attcattaac cctaccgggt gctgcgaaag gcttagatgc gttatttacc ccaaactggg
      721 aaaaattggc tgatccaagt gtgtggattg ccgcttacgg tcaaatcttc ttctcactct
      781 ctatctgctt cgggattatg attacctatg cttcatacct gaaaaaagaa accgatttaa
      841 ccggttcagg cttagtggtg ggttttgcga acagtggttt tgaattatta gcgggtattg
      901 gcgtatttgc ggcactcggc tttatggcaa cggctgcagg gcaagaagtg aacgaggtag
      961 caaaaggcgg tatcggcttg gcattcttcg ctttcccaac cattattaac caagcaccaa
     1021 tgggggcatt agttggcgtg ctgttctttg gttcactcac ctttgcggca ttaacctcat
     1081 ttatctcggt aattgaagtg attatttcag cggtgcaaga caaactcaat tcaggtcgtg
     1141 tgaaagccac gctactagtc ggcattccga tgatggtggt atctgtcttg ctattcggta
     1201 caaccaccgg tttaccaatg cttgatgtga tggataaatt cgtaaaccaa ttcggtattg
     1261 ttgcggtggc gtttgtttcc ttgattgcga ttgtcaccag cgagaaatta agcacccttg
     1321 aaaaacacct gaacgaagtt tcttcattca aagtcggctt tatctggcgt ttatgtatcg
     1381 ttattacctc cggcgtactg gcatttatgt tattcagcga aatcgccaaa gtgtatcgtg
     1441 aaggttatga aggctaccca agctggtttg taaacacctt cggctgggga atggcaatcg
     1501 gcttagtggt ggtggcttat ttgctatcaa aaatggcgtg gaaaaatgaa ccaaaaggag
     1561 atgagtaatg agttcagcag caattattac catggtggtg gcaatgacga ttatctgggg
     1621 cggtttactt tttgcgatta aacgtctgcc gaaagaagaa caataatttg caaatttttg
     1681 tataaaaaag cccgcttgtt agcgggcttt tggctatacg gtaatcccaa atttctcttc
     1741 cacaaaggct tggaaatcgg tgcaaccgcc aacgtggtta ttatccacaa aaatttgtgg
     1801 tacagttgca accggcttgc caacacgagg ctctaggtct tgttttgaaa tattttcagc
     1861 gatcatatca ataaatttaa aatcaaaatc gcttaattct tgtttcattt tctcggctaa
     1921 tgtttttgca cgcacgcagt atgggcaaga taaacgtcca taaatttcaa caaacatata
     1981 atgttccttc tataaagtaa aagtgagcga attgtccgtt aaacggctct gtgtaagtgt
     2041 aacaattttt tcaacctttt aggataaatt tcttcaccat cacggcaagc agttaatgtg
     2101 atatactggg gcaaatttac aagtagagaa acagagatga cccaattaat gacgtttgag
     2161 gagcttgatt taagccctca attacttaaa gccttagcac acaaaggcta taaccgccca
     2221 accgctattc aacaagccac gattccggct gcgttagaag gacgtgatct gctcggttca
     2281 gcccctaccg gtaccggcaa aaccgccgct tttttactgc cggcaattca gcatttatta
     2341 gattatcctc gccgcaaacc aggggctcct cgcattttaa ttcttacccc aacccgagag
     2401 ctggcaatgc aggtggcgga gcaagcccaa gcactggcta aatttactca gttgagcatt
     2461 gcgactatta ccggtggggt ggcgtatcaa aatcacggtg aagtctttaa taaaaatcaa
     2521 gatattgtgg ttgctacccc gggtcgttta atgcaataca ttaaagagga aaattttgac
     2581 tgccgagcgg tggaagtgct gatttttgat gaagcagata gaatgttgca aatgggcttc
     2641 gggcaagatg cggagaaaat tgcggcagaa acccgttggc gtaagcatac ttggttgttt
     2701 tcagcgaccc tcgagggcga gttattagag gattttgccg agcgattatt aaatgatccg
     2761 attaaagtcg atgccgaacc aagtcgccgt gagcgtaaaa aaattcagca atggtattac
     2821 cacgccgata atgtagagca taaaaccaaa ttattagctc gtattatcag cgaattttcg
     2881 gtggaaagag cgattgtgtt tgtccgccgc cgagaagatg tgcgagaact cagcgaaacc
     2941 ttgcgtaagc ggggaattcg ttctacttat ctcgaaggtg aaatggctca gacccaacgt
     3001 aaccaagcta ttaaccgttt aaaagaaggc gtagtgaatg tgttagtggc aaccgatgtg
     3061 gcggctcgag gcattgatat agacgatatt gatttcgtgc tgaactacga tttaccttat
     3121 agtgcggata cttacttgca ccgtattggg cgtaccgctc gtgctggcaa aaaaggttcg
     3181 gcaatttcat tggtggaagc tcacgattat aaattactcg gcaaaattaa acgttacacc
     3241 gaagaagtac taaaacctcg cattatcgag ggcttagagc ctcgcaccaa agccccgaaa
     3301 gatggcgagc taaatgtgac cagcaagaaa gaaaaagcac gcattaagaa gaaaaaagag
     3361 gcgaaaaaag aagcggcaaa accaaaagta aaagtccgcc acaaagatac caaaaatatc
     3421 ggtaaacggc gtaaaccaag tagtgaatcg gctgcggtta aacaggcata atgatacaag
     3481 cggttatttt ctgaagattt tttgcaaaaa tttcatcaaa aataaccgct tgtttatatc
     3541 taccttactg ccatttggaa cagaatcact tctgcttggc agctgaaagt gatccacatt
     3601 ttcatcagaa acccttctgc aatttgtgta atttcgcttt gaatttcaca aggttcggtt
     3661 tcgaccgatt ttgctttttg tgcgaaaaat tcgaccgctt gttgagccag ttcttcgctc
     3721 tcatacaccg cttcaaaggc aatttggcgg tctgcattat ccaacataga atcgcctttt
     3781 agccggcaca tattatgtgc ctcagccgct ttttgttgca tcgttttgct catattacac
     3841 cttatccgta atttgtttta accaagtgta ggattgatgc tgtttttgct catcgaaaat
     3901 gtagtttacc gccataattt catcaatttc cactcgtgct tttagggctt taagttgttg
     3961 ttcaacgctt tgcgggctac caattaagga gcattcggtc atttggcgga caacggtttt
     4021 ctcttgtcgg tatagagcat tttgctcaaa ggcaaccggc ccgaaatgcg gcacaatttc
     4081 tgctttcaca aaattttccc acactttgtc gtcagattcg agcggtggtt gcagattttg
     4141 ttgggcattg gtcactacat ttaagaaaaa ctgggtttgg gtggtcgcca gttgtgttgc
     4201 ttcttcatcg gtttctgcca caatggcatt cacacctaaa atcacataag gtttatctaa
     4261 cacatcagag ggcttaaaat gtgagcggta aatgcggatt gcatcgtcca taaagcgagg
     4321 ggcaaagtgt gaagcgaagg cgtaaggcaa gcctcgttca gccgctaaat aggcactttc
     4381 agggcttgag cctagaatat aaaacggcac atttagccct tgtgccggat aggcggaaac
     4441 agggtttgta ccgtcaaagt agccttttaa ttcgctgatt tcatcgggga aaggcaaact
     4501 ctgggcgtaa ggcagaggcg gttcgcatat cggtgccggg ggctcgccca agccccagtt
     4561 ctactcggtt tgggtagagt gtttcaagcg tgccgtattg ttctgccaca atataagggc
     4621 tgtggttggg taacatcacg ccaccggagc cgactcttaa tttttcggtg tgggagaggg
     4681 cgtgttgaat caataattgc gtggcggaac tggcaagatt tttcatatta tggtgttcgg
     4741 caatccaata gcgttcaatg ccgatatttt ctgcgtgttt tgctaatttc accatagaat
     4801 cgaccgcttg tttggcagtt tgcccttcac gaatcggcac aaggttgaga atggataagt
     4861 ttgtcatttt gttctccgag tttgcatata aaaaaggctg aatagcttta ctattcagca
     4921 cttgtaaaac catctcctta atatggggac gattatttct tactcaagaa gaccacagcg
     4981 tcaatatagc gttggataaa atcattggtc gagcttgaat cggaaaatcc ttctaggtta
     5041 agctggtatt gaccattcac gaaaaacgcc ggtacaccac gaatttggaa gtcttcagct
     5101 aattgaactt gtttattgac taaaccgttt actgcaaagc tgttgatacc gctgtcaaac
     5161 tgttctgcac taatgctgtt agcgatgaag accgcacgaa tatcgtccat tgatttaaac
     5221 gcatcttttt gagcggcttc aaacaaggcg gtttttactt tatcttccgc tcctaatgcc
     5281 attgccaatg cccaagcacg ggttaagttt tcagattggc gacctaagaa attgacgtga
     5341 tattgcacta atttgctgtc tgcaggcaat ttcgctttga tttggcttgg aattttgtaa
     5401 tttagctcaa aatcgtaaca gtgggggcag tagaatgaga aaaattccac cacttctttt
     5461 tgagccgatg gggctttacg cacctcaata tactctttgc cggcagttgg atctgctgca
     5521 actgcggttg cgttaaatgc aaaaagggca gaaagagcaa ccagtacggt ttttaatgca
     5581 aattttttca taatgattcc ttcaaaaata tgataagtga gaaataagac aaacttaatc
     5641 gtttaaaatt ccacgagctc ggagcaatgc ggttttaaaa tcatcttcat aatctttttt
     5701 aatgcccgga atcggctcaa atttatcggc gtttcgcatt ttgagctgat aaatcaaaac
     5761 ctcgtcacgc agggcatgaa aattatccgg ctcgcttact tccaccgaaa gctgttgcag
     5821 aatatcaatt aagtgaagat cggggttttt ccgccaatgt tcgccaagca gttccaatag
     5881 ctcttctaaa cgtttgcatt tcatagtatg cctcttaaaa aacttgttga atagtaagcg
     5941 gaataatata cttttagccc tcatttggca aaaaaattca ggtttggaga gggaaatgga
     6001 agagaaaatt tcagcggtaa ttttagccgg aggtttggct cggcgaatga atggagcgga
     6061 aaaagggttg caactgttag atcaaaaacc gttgatttcg cacctgttag cccgactttc
     6121 gccacaaatc gaggatattt ggctcaatat caatcgttcg gtggaacaat atcaacaact
     6181 ttaccctgaa ttaccttatt accaagacag cttgcccgat tttcaaggcc cactaagtgg
     6241 aatgctttcc gggttcgagc agattgagag cgactattta ctgtttgtgc cttgtgacac
     6301 gccgtttatt ccaacccaac tattgcacaa gttacacacg gcattacgca ttaatcaggc
     6361 tcaaattgcc tatgctcacg atggcgaccg cccacaccca acggttgcct tggtgcatcg
     6421 ttcggtggcg gaaaatttaa gagcgtattt agcctccggc gaacgccgat tattgtggtt
     6481 ttttcaaacc caaaaaagtg tggcagtgga tttctcggca tataaagccg catttcgcaa
     6541 ttttaattgt ttagaagatt taacggcttt caatccatat cccatcaaaa ccctggcgat
     6601 tactggctat agcggcacag gcaaaaccac cttattggaa aaattaatcc caaaattgac
     6661 cgcttgcggt attcgggttg gcttgattaa gcattcccac cacaatgttg atattgataa
     6721 aaaaggaaaa gacagctatc gcttgcgaga agcaggagca aacccgacta tggtcgtgtg
     6781 tgatgaacgt tgggcattaa tgacggaaac taagcaggca gcagaaacta aacaagcggt
     6841 cgaattttca caattaattg caaaatttga cccagagacg gtagatttag ttttagtgga
     6901 aggctttaag cacgaaacaa tgccgaaaat tcaactacac cgtaaagaaa ttgaaaaacc
     6961 gttgccggcg ttagaccagt ggacaatcgc gaccgcaacc gattatccgc ttgagcggga
     7021 aaatcggttg gatattaatg atgttgaaca gcttgctgat tttattcggc agtggctaga
     7081 ggcaagcaaa gagccggcta attaattgac taaaaagccc ctaaagtaag cgattgctta
     7141 gtttaggggc taatagcaat taccagctat atcccagttt gaagccggca aaatgttgtt
     7201 tgccgatttc gttacctttc aatagaccaa ggtgagagct taattgccat tgaccttttg
     7261 taacacccac gcccacttca gttttcagat aacgtccgaa acgttggcta aaggtattgt
     7321 cattgactgc cacactcaag cgtttgctgc tggcatcgac ataatgtgcg gcaaggctag
     7381 gctcaacttt ccagctggag aggtcaaagg ccttgctgac tttcacacct gcttggtagg
     7441 ccataaagtg tgctttcggg ctgcggactt ctacttcacc cagtttgtaa ttttgtgcgc
     7501 tgaagcgata gtaacgtgta ccaacagcag gctgaacttg tacgccggct aagtcgaagt
     7561 tatgtccaag atttgcccca atcgccataa tatgacggtt aaagcggttc tccccgaaca
     7621 ggtcgatacg gtttttcgct ttaccgtagc taccgtctag gctgacgaaa gtgccgtttt
     7681 ccgaatgcca tttaccatat aggcttgcca tcagtaagtt ggatttaccg ttcactccct
     7741 catcaaattc cgcattagcg tggttgcggg atagcatcac accggcattg ataccgtttg
     7801 caagcggtaa ttctatgcct aactgagtga ggttggtttg ttgctcatac gggcgatata
     7861 aatccgattg gtgttcggtt ttttgatgtt cgatactgct ccaaacgtgg aatttatcgt
     7921 gttttgcgaa cagttggcga tctaagtctt gcccgatttg caacgcagaa tttgcctgtg
     7981 ccgatagctc ggaaagagcg gtattggcat attggcttac ccagtcgctt tgttgaatat
     8041 tactactgtc agtgtctgcg tgtgcttgta gatgctcaca atttgcacca ttctcttggc
     8101 aaagttttag tgcctcagcg tttaaaattg cttcactatt tccagcagtt atacgtaagg
     8161 tagctaattt tgcttctact tgttttgcaa tattttgtgc agtttcaacc gcttgttcta
     8221 cgttagcaat ttgcgcagcg gatgctttag cattgttttc tgctgtagtg agtgcagcac
     8281 tcgcttgtgt gactttctgt tttgctaccg taatttgccc ttggatttgg cggtaatacg
     8341 ttggatagta acggcggtag taatcagcat attgttgcaa acgggttaat tcgctatttg
     8401 cttggctaag ctgagattgt gcgtttgcag cggcttgttt agcatttttc tctgcttggc
     8461 gctctttctc ggcttctgcg ttcaaacggc tgatttcctg tttttgttta tccgcttctg
     8521 caatagcatg gtctaattcc gctttcgccg ttgcaatcga ctgattccga tctttagcat
     8581 cttttaacgg attgtataag cgatagccgt tgcttcgatt tgccagcaca taacgatatg
     8641 cacctaaatc aacataaccg ttttccagtg aaactttagc tttatcagcc tgagcctgat
     8701 gggttaaggt taataaactt aacgggctaa ccacttcagc ttctttgcca ctgttgcgta
     8761 atgcaagagt gaaagtcccc tcggcgagac catttacttg taggtgatcg cctttgccag
     8821 cagcaatatc ggttaagtag ttgattttgg cattaccttg gaaattaccg tcaataatca
     8881 acgtattaaa acgagtcggt atctcgcctg ttacaaattt ttcattcagg ttgatggttg
     8941 aaccgttttc tgccactaaa ttgccagtac ggctatcccc agtgtttgtc cagcttgctt
     9001 ggttagctaa acgaatgctc gttgttgctg ctgcttgaat ggtgccaatc aggttcgctt
     9061 tgccgatgtg taattggctc tgttctgcca agctgacatc accattaatt tgtgttgtgg
     9121 gcagtgttgc aaaaatttca ttagaaacga ccgcttgtgt atcacagctt gttttaccag
     9181 tgtactcaga atagtagcaa cttggagttt gctcgttgat aaagcctaaa tgtagctgag
     9241 catggtcatt cgctgacaag tttgcttgca ggtttgagac attacgacca acataaagct
     9301 gagcgtattg gtttagctgc atattatcag ccttaaattg acgattttgc caatcatcgg
     9361 cataaactac atcttgctta ttgatcgcat cataagcgtg aggggtcggt ctgccggaaa
     9421 gtagcacttt acctcctttc acagttagat cgccgtttaa gttcaaacca ccagagaagg
     9481 tataaatact attgagcttt tcttgctcta ttggtgcgtt tttacgggct aatactttgt
     9541 tgatcgcttg attgcgatct gcagctaaaa attcccaatg ctcgctgctt aagccaccgc
     9601 gaggaacagg ttgttttgga tcacctttta atgcaaagta ttcccgaatt ttcgttttcg
     9661 gattggtgaa cagataaata tctgcaccag ctactagccc cttaccccaa gttacttcag
     9721 aggcagttgc cggtggtgta gaggcttttg gatcatacac cacattcaaa cgaccgttat
     9781 gctgggtgtt atctgtttcg ccaataaaac cattaaagct attgtagcga tgctccagac
     9841 ctttttgcgc gagtacttgt tttactgctg catccttgtt agaagacagg aaggtccaat
     9901 gggcattact tgcgccattc gttggcatat attggtttgg attgcctttg agcgtaaagt
     9961 aatcggtacg gttattagca tgtgggttga tatattcata aatatcagca ctgttttctt
    10021 tccacgttcc ccattttaag tccgttgctt cagggggctt cgggccatta atggtgagtg
    10081 ttgcagcttt ttgtaggtga ttattaacaa tacgcgcacc atcatcacta ttttgaatac
    10141 gattaaacgc aatgttgttg ccgttgagat ctaaacgacc accacggaag ccaaagtaaa
    10201 tcttatttgg atcgacttga tcagcactgt ttaatacaac ggttgaacgg ccgctcacaa
    10261 taccaacttg gttaaatgca gattttttgc cattttcacc cgcttgttgg gtcaaaatga
    10321 cagtgccttg tccaacacta atgtcaccaa ggttttcgcc tttaccgtta atatgtagtg
    10381 tcccttcgcc caatttagat aaacggtcac cttgtgggtt tttgacttgc caattaactt
    10441 gtttaccttt tgccactaca attccagctc ctacccagct ttgatcattt tcagggcgaa
    10501 ctgttgcatt agcattgaaa tttaatgcgc cagcaccttg gtcaatatcg gtttttaata
    10561 cgatagtgct atcttgtcct tcaatgttta gggtttttcc ggtgttatct tgcggaagtt
    10621 gtagctgagg tgtgccacca tagcctaatg gagaagcgct attgtcttta attgaacgat
    10681 cagcaagcga aaccgttaag tttttccctt tattaccaat gctactgtct ttaccctgtg
    10741 cagaccattc aaaaacggca ttttgttgcg cattggttaa tgtgccggca atatcttcgg
    10801 caaattgttt atcgtgataa tcctgacgga taataccggc acggttgtag tagttgtttg
    10861 caggcgtacc aaaggtgtaa gtagccagta gtagccagcg tttcgttttc tgatcgtagc
    10921 catacacacc tgaaccgcta tcaccacgag taccaaatgc agttaatgct ccatatgggc
    10981 tattgtttaa tgcatttcct ctaaaatcta gccagtaatc ggaattatgt accgcgattg
    11041 gtaaatgaac tcccccggtt aaatattggt aagatgttga aatttggttg tctttatgct
    11101 cggtattttt gactatttga cgaccagagc ctaagcgagc aaagtgagta aaacgactac
    11161 tatcttggta ggctgaagca ttattaccca gatcagtcac tgttgcaggt gcgacctcag
    11221 tgactaattt attgagacgt ggcacttgat aatcttgatg ttgcccacct tcaacaattc
    11281 catagtcgtt acgatcaacg actttgtaag tgtagtggtg tgcatcggca ttagtgcctg
    11341 gggcgccaaa ttccacagta ttgtattgtg tattatgggc aacgctgact aaatattgcg
    11401 gtgcaactag agttgctatc cctaagttac ggtttgcagc actaaaatct ggcatcggta
    11461 catttgggaa gagtgcatct aatgtattgc cacttttatc tttaatttca atattgcttg
    11521 cacctacggt gaatttaccc ttattctccg caaaatcacg gtagtattga agatcgatat
    11581 catctctgaa aacagaggct gttgcagata aactaacagc aaatgccaca aagc
//
LOCUS       DANLXF010000066        11305 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.066, whole genome shotgun sequence.
ACCESSION   DANLXF010000066 DANLXF010000000
VERSION     DANLXF010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11305
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.066"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..214
                     /locus_tag="PXD66_001143"
     CDS             <1..214
                     /locus_tag="PXD66_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDL6319453.1"
                     /translation="ILKRKSQKTLQQIYTQPISANIKWTNIEALFLELGAEIEEREGS
                     RIAVVLFQQVRVFHRPHPNPDTDKGR"
     gene            257..586
                     /locus_tag="PXD66_001144"
     CDS             257..586
                     /locus_tag="PXD66_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDL6319454.1"
                     /translation="MRNIMDINGYKAVIAYDPETELFRGEFIGLNGGADFYADNVIQL
                     KKEGELSLNIFLELCREKGIEPFKQYSGKFNVRLSPELHKAAVIAATAENLSLNEWIN
                     QTLEKSV"
     gene            complement(625..1011)
                     /gene="cueR"
                     /locus_tag="PXD66_001145"
     CDS             complement(625..1011)
                     /gene="cueR"
                     /locus_tag="PXD66_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HDL6319455.1"
                     /translation="MNISQAAEKTGLSAKQIRDYEKAGLLPPTSRTEAGYRFYNATDL
                     SRLHFISNARKVGFSLAQISELLKLNDNPNRSSRDVKQLTEQHIGELEQKIADLNQML
                     TLLKSWSSSCCGNANSECSILNGLNG"
     gene            1100..1300
                     /locus_tag="PXD66_001146"
     CDS             1100..1300
                     /locus_tag="PXD66_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDL6319456.1"
                     /translation="MTITLQLDGLHCGNCVKSVEKALNGVAGVNQVSVTLEPQIAVVE
                     GSAEAQALIEAVEDIGFDAKLA"
     gene            1353..3545
                     /locus_tag="PXD66_001147"
     CDS             1353..3545
                     /locus_tag="PXD66_001147"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005710411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDL6319457.1"
                     /translation="MTSTAHYSPEQQLLIDGMHCAACVRRVEKALLKVEKVEFASVNL
                     ADQTAFVQGNANPEALVAAVVKIGFGAEVLESEEERRIKQQAQTQRVLGHKRTQFMLA
                     LVVGFALVAFGFYKGMTVNETNRSLWLSWAGISLATMYFSGKDFFLGAFSALKNRSAN
                     MDMLVAISTGTAWLYSCWLTLFPQPNAHVYFEASVMILGFINLGKYLELKAKQRSSLA
                     LEKLLDLAPKQAVIFEENIAKTIPAKAIKPQMRVQALTGDRLAVDGILDSGSIWVDES
                     MLTGEALPVEKKIGDKVRAGTLVQDGSGTYIAEQVGSQTALARVINAVRHAQSSKPPL
                     AQFVDKIAAVFVPVVVSIALLSALIWLAVGQDFAFALSIFTTVLIIACPCALGLAIPL
                     STIAGVARSAEFGVLVRNIEALQASSEIDTLVFDKTGTLTTGRMEVSDVLTFNGFEQK
                     RLLQLAKSVEQHASHPIAKAIAKFAENQTACGVTDVQVLKGLGISAKYEGSTIRMGNW
                     QFVGETIQANLASLLPAKEGDFSSKIFVSVDNQLAGVLSVSDQLRPEAKATIHQFQAE
                     GYQCVMLTGDRQQTADYFANALGLDGVIAEVLPEQKAEKIRALQAEGKKVAMIGDGIN
                     DAPALAQANVGIAMHNGSDIAVETADLSLMREGLAPLMQILPFSKKVLKNMKQSLLGA
                     FIYNVIAIPIAAGVLYPFTGWLLNPMIAAIAMTLSSITVVLNSQRLLK"
     gene            complement(3598..4383)
                     /gene="truA"
                     /locus_tag="PXD66_001148"
     CDS             complement(3598..4383)
                     /gene="truA"
                     /locus_tag="PXD66_001148"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDL6319458.1"
                     /translation="MKIALGIEYDGSRYFGWQRQEAVESVQQQLEQALSVVANSPVEV
                     FCAGRTDAGVHGTGQVVHFETQANRPLQSWCFGTNAHLPDDIAVKWAVAVNEDFHARF
                     SATARRYRYIIFNQKLRSAILPKGVTHFHFPLDERKMHQAGQYLLGENDFSSFRAAKC
                     QSNTPWRNIHHLNVSRQGNYVIVDIQANAFVHHMVRNIVGTLLEIGQGRQPVEWAKWV
                     LEQRDREKAAPTAKAEGLYLVEVHYPEHFGIPKTPLGPLFLAD"
     gene            complement(4448..5137)
                     /gene="folE"
                     /locus_tag="PXD66_001149"
     CDS             complement(4448..5137)
                     /gene="folE"
                     /locus_tag="PXD66_001149"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HDL6319459.1"
                     /translation="MTTISPELTITENTLSPEAQKVRQALLAKGIETPTVTHTKDKAS
                     RRAEIQQHMQAVLELLGLDLSDDSLEETPHRLAKMYVDEIFSGLDYATFPKITNIENR
                     MKVSEMVLVDDITLTSTCEHHFVTIDGKVAVAYYPKKWVIGLSKINRVVQFFAQRPQV
                     QERFTEQILTAFQTILETDDVAVYVKATHFCVKCRGIKDTNSYTVTSAFGGVFLEDRE
                     TRKEFLGLLNK"
     gene            5238..5945
                     /locus_tag="PXD66_001150"
     CDS             5238..5945
                     /locus_tag="PXD66_001150"
                     /EC_number="2.7.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid kinase"
                     /protein_id="HDL6319460.1"
                     /translation="MQNIQYLFNADLIPEHQAEFVQSLLTCKNFDNSDRLLGFSKGRG
                     TTWFLDTSAELGINVVLRHYYRGGLFGKIIKDSYFFTRFEATRAYKEFFLLQQMLAWG
                     LPVPRPVAIKIERHFCCYRADIMLEKLENTQDLSKILQSHSLSNEQYEQIGKLIRSLH
                     NHQIHHSDLNIHNILLGNDGKFCLIDFDKCGIEPGENWKQNNLDRLLRSFKKEQGRLN
                     IFFSEENWQAVLRGYTL"
     gene            complement(6000..7097)
                     /gene="apbC"
                     /locus_tag="PXD66_001151"
     CDS             complement(6000..7097)
                     /gene="apbC"
                     /locus_tag="PXD66_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HDL6319461.1"
                     /translation="MNQLNEQQLVEIKAILQQFSHPTLQKDLIALNAFKKAELGGDIL
                     RIEITMPFAWNSGFETLKAETEAKLKQVTGSSGVKWVLNYQIATLKRANKHPAVNGVK
                     NIIAVTSGKGGVGKSTTSVNLALALRAQGTRVGILDADIYGPSIPHMLGAKDQRPTSP
                     DNKHITPITAHGLQSNSIGYLMEEDNATIWRGPMASSALSQLLNETWWNELDYLVIDM
                     PPGTGDIQLTLSQQIPVTGAVVVTTPQDIALLDAIKGISMFEKVSVPVLGVIENMSMH
                     ICSNCGHHEAIFGTGGAEKIAQKYGTKVLAQLPLHIRLREDLDSGVPTVVAAPEHEIS
                     QAYLALAEKVASELYWQGSVIPSEIMIREVK"
     gene            complement(7094..8641)
                     /locus_tag="PXD66_001152"
     CDS             complement(7094..8641)
                     /locus_tag="PXD66_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL6319462.1"
                     /translation="MLKSLQAVTFVKKIAKVTACILLITSCDQLICPQSIQSVWVENR
                     QSDTLLKRAIYSAVFQLDPHKIKASADAAPLRDLLVGLTAYNRKGDVVPAIAQNWFTE
                     DEKEWLFILDEKAVWSNGQPVTAQDFVASWRRLIDPQNHSVLSKYLIYMGIENAKAIE
                     EQIKSVEELGVEALNAHTLRIRLNHPNHQLPEMLAHSALLPTYQGIAPTIDNFVSNAS
                     YRLEKLDAQKMVLKARTQEIPFQTIEYDLITTIQNCSAFDIVENPLLNKKTNIVKFPR
                     LCSYYYEFNFDDPLLAKREIREAIRTMVASANSGQEYGVPSQSVIPHSLVKDREKRWN
                     PVVVEQLLTKAGISPYNPLKLTLLYDENSINIANQVTRALSQSELFNITPKMVDWQTL
                     NKVREQKDYQIARAGWCADYPDPIVFLQKFHSMSPDNHSGYKSEIVDQKLNRLQTETL
                     SAEARESLIQEISEQLYSDVAVLPLFQYHHRVGIVSSLLGIDLNNDSEVIYSKDLKRN
                     LANKDNQ"
     gene            complement(8667..9317)
                     /locus_tag="PXD66_001153"
     CDS             complement(8667..9317)
                     /locus_tag="PXD66_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchE family NAAT transporter"
                     /protein_id="HDL6319463.1"
                     /translation="MDIIINFAIYLQFFIGLFAIVNPFGSLPIFFSMTAHQYEGERNR
                     TSLITAVSIGVILLVSLYFGNLILNAFSISLSSFRVAGGILIVSIAMTMISGKLGEHK
                     QNKEEKNEDITEYENIGVVPLAMPIMAGPGAIGSTIVWGTRYHTWEDYVGFSIAIIVF
                     ALICYILFRYSAPLVKKLGKTGSNVVTRIMGLILMALGIEIIVAGITTLFPVLLVK"
     gene            complement(9439..>11305)
                     /locus_tag="PXD66_001154"
     CDS             complement(9439..>11305)
                     /locus_tag="PXD66_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HDL6319464.1"
                     /translation="EKMHERTNGPVFKIIFALVSISFVIGGIGTGLISQDNSVAKVNG
                     EEISQQLFNNTLNREQNRLNAELGSRFWDLMDSPEYAAQFNQSVLNGLIDEELLRQYA
                     KELKLGISADQIKSEIVNSQMFQQDGKFSNELYQQTLRHNNLSADGYAAIVQEGMLQS
                     QLQEGIINSDFNVPAQQALLAKLLLQQREVRLAEFSVAKEMENQTASEQELRAYYEAN
                     KTKLLAPEKLAVEYVTLSPKDVESKVEITNEQIQTYYDRNKADYVTKGEAHLAHIQVA
                     SEAEAQAIEQALKNGEDFATLAKNKSSDTLSANNGGDLGWAKAGTFPKAFEDAVANLT
                     AGQMSAAVKVDNAYHIIKVLERKPEQVIELAQVKDKIAQTIRQELVLTEYFTVAREMA
                     NKAFENNSSLEEVAKAGNVELQKTDLFTRENVPTALQNEKVLKVLFEGELRQNKQNSD
                     ALDVGNDANPQTLFVRVSDYDAERPQTFDEAKAAVENFVKAEKAEKALLAKVEENVKA
                     LNEGKSVDVKFNAPQTLVYMQAEVENPVLAGTIFAMKKAQDKTTYQMARNQKGDVVIV
                     ALDNITEGNAEQFKTLAPQFEQANRLILRNEFLQSLRAKASIEVNEDFMQQRSSH"
BASE COUNT         3214 a         2434 c         2253 g         3404 t
ORIGIN      
        1 aatcctaaag cggaaatctc aaaagacact acaacaaatc tacacccagc ctatttctgc
       61 caatattaaa tggacaaata tagaagctct ttttctagag ttaggagcag aaatagaaga
      121 acgagaaggg agcagaattg cagttgtttt atttcaacag gtcagagtat ttcatcgccc
      181 acacccgaat cctgatacag ataaggggcg gtagcgagta ttaaaaaatg gttatctgaa
      241 aacggagtca ttccttatgc gtaatattat ggatattaat ggttataaag ctgtcattgc
      301 ttacgatccc gaaacagaat tatttcgagg tgaatttatc ggattaaatg gtggagccga
      361 tttttatgcc gataatgtca ttcaattaaa aaaagaaggt gaactaagtt tgaatatctt
      421 tttagaactt tgtagagaaa aaggtattga gccgttcaaa caatattccg gaaagtttaa
      481 tgttcgcctt tcgcctgaat tacataaagc tgcggtaatt gcagcaactg ctgaaaattt
      541 aagcttaaat gaatggatta accaaacgtt agaaaagagc gtgtagccta attgcaaaac
      601 aatcacctat tttgaccgct tgtattaccc gtttaaccca ttcaatatcg aacactccga
      661 attggcgttg ccacagcaag agctactcca gcttttgagc aaagtcagca tttggtttaa
      721 atcggcaatt ttttgttcaa gctcaccgat atgctgttcg gttaattgtt ttacatctcg
      781 gcttgagcgg tttgggttgt cgtttaattt aagtagttcg ctgatttgag ccagcgaaaa
      841 gcccactttt cgggcattgc tgataaaatg taatcggctc aaatcagtag cattgtagaa
      901 tcgatacccc gcttcggtgc gacttgtcgg cggcagaagt cctgcttttt cataatctcg
      961 gatttgtttg gcggaaagcc cggttttttc tgcggcttgg ctaatattca ttataatcct
     1021 ctcactttaa tcgcttgacc ttaacctaag gttaagcttt attatacgcc tgaataatca
     1081 ctcaaaacag gagaaaatga tgacaattac cctacaactt gatggcttac attgtggcaa
     1141 ctgtgtgaaa agtgtcgaaa aagcgttaaa tggcgtggct ggggtgaacc aagttagcgt
     1201 aacgttagaa ccgcaaattg ctgtggtgga aggctcagca gaagcacaag ccttaattga
     1261 agcggttgaa gacattggct ttgacgcaaa attagcctaa ttaataaggg gcagaaattt
     1321 tgccctaatc tgcccaatat ttcggaggat aaatgacaag cacagctcac tattcgccag
     1381 aacaacaact tttgattgat ggaatgcact gtgccgcctg tgttcggcga gtggaaaaag
     1441 ccttacttaa agtggaaaag gtggaatttg cctcagtcaa tcttgccgat caaaccgctt
     1501 ttgtgcaagg caacgccaac cctgaagcct tggtagcagc ggtagttaaa atcggttttg
     1561 gggcagaagt gctggaaagc gaggaagaac gccgaattaa acaacaagcc caaacgcaac
     1621 gagtgttagg gcataaacgc actcaattta tgcttgccct tgtagttggt tttgccttag
     1681 ttgcttttgg gttctacaaa ggaatgacgg taaacgaaac caatcgttcg ctttggctca
     1741 gctgggcagg tattagcctt gcgacaatgt atttttcagg taaagatttc tttcttgggg
     1801 cattttcagc actgaaaaac cgctcagcca atatggacat gttagtagca atcagcaccg
     1861 gcacggcttg gctctattct tgttggctca cgcttttccc acaaccgaat gctcacgttt
     1921 attttgaagc cagcgtgatg attctcggct ttatcaatct aggaaaatac ttagaactga
     1981 aagccaaaca gcgttcctcg ctagcattgg aaaaattgct ggatctcgcc ccaaaacaag
     2041 cggtcatttt tgaagaaaat attgcaaaaa ccattccggc taaagcaatt aagccacaaa
     2101 tgcgggtgca agccttaacc ggtgaccgct tggcggttga tggtatttta gacagtggct
     2161 ctatttgggt tgatgaatca atgctcaccg gtgaagccct acctgtagag aaaaaaatcg
     2221 gtgataaagt gcgagccggc actctggtgc aagacggttc aggcacttac attgccgaac
     2281 aggtcggctc acaaaccgct ttggctcgtg taatcaatgc cgttcgccac gcccaaagca
     2341 gcaaaccgcc attggcacag tttgtagata aaatcgccgc tgtttttgtg ccggtggtgg
     2401 tttcaatcgc attactttct gcgttaattt ggctggcagt tgggcaggat tttgcctttg
     2461 cactctcgat ttttactacc gtgttgatca tcgcttgccc ttgtgcattg gggctggcaa
     2521 ttccgctttc taccattgcc ggcgtggcac gttctgccga atttggcgtg ctggtgcgaa
     2581 atattgaggc actacaagca agcagtgaaa tcgacaccct agtgttcgat aaaaccggca
     2641 cactcaccac tggcagaatg gaagtgtcgg atgttctcac ctttaacgga tttgagcaaa
     2701 agcgcttgct acaacttgct aaaagtgtag agcaacacgc ctcacacccg attgcgaaag
     2761 cgattgcaaa atttgccgaa aatcagaccg cttgtggtgt aaccgatgtg caagtgttga
     2821 aagggttagg catttcagca aaatatgaag gcagtacaat cagaatggga aattggcaat
     2881 ttgtaggcga aaccattcaa gcaaatcttg cctccctatt gcccgctaaa gagggggatt
     2941 tttcctcaaa aatttttgtt tcggtggata atcaactcgc cggtgtattg tcggtttcag
     3001 accaacttcg ccctgaagcg aaagcaacca ttcatcaatt ccaagcggaa ggctatcaat
     3061 gcgtgatgct gaccggtgat cgccaacaaa ctgccgacta tttcgccaac gcattggggt
     3121 tagacggcgt gattgcggag gtattaccgg agcaaaaagc ggaaaaaatt cgagccttgc
     3181 aagcagaggg taaaaaagta gcaatgattg gcgacggcat aaacgatgct ccagctctgg
     3241 ctcaagccaa tgtgggcatt gcgatgcaca atggttcaga tattgcagtg gaaaccgccg
     3301 atctttcgct gatgcgagaa gggctagcac cactgatgca aattctaccg ttctctaaaa
     3361 aagtgctgaa aaatatgaag caaagtttgc tcggggcttt tatctataat gttattgcga
     3421 ttcctattgc tgccggcgtg ctttaccctt tcaccggctg gctactcaac ccaatgattg
     3481 ccgccattgc gatgacattg tcgtcaatta ccgtggtgtt aaatagtcaa aggttgttga
     3541 aataatttaa gcggtcaaat ttgcatattt ttttgcaaat ttgaccgctt gtaatcacta
     3601 atccgccaaa aacagcggcc caagaggtgt tttcggaatg ccaaaatgct cagggtaatg
     3661 cacctcgacc aaatatagcc cctcggcttt cgctgtaggg gcagcttttt cccggtcacg
     3721 ttgctccaac acccattttg cccattccac aggctgtctg ccttgcccga tttcgagcaa
     3781 tgttcccaca atattgcgaa ccatatgatg tacaaaggcg ttggcttgaa tatcaacaat
     3841 cacataattg ccttgccgag acacatttaa atgatgaata ttccgccacg gcgtattaga
     3901 ttggcatttg gcggcacgga aagaggaaaa gtcattctcg cccagcaaat actgccctgc
     3961 ttggtgcatt ttgcgttcat caaggggaaa gtgaaagtgg gtaacacctt taggtaaaat
     4021 tgccgaccgc agtttttggt taaaaataat atagcggtaa cggcgggcgg tggcactaaa
     4081 acgggcgtga aaatcctcat tcaccgctac cgcccatttc accgcaatat catcgggtaa
     4141 atgagcgtta gtgccgaaac accagctttg tagcgggcgg tttgcttgcg tttcaaaatg
     4201 cacgacctgc cctgtgccgt gcacaccggc atcggttctg ccggcacaaa acacttctac
     4261 gggcgaattg gcaacaaccg acaacgcctg ctctagctgt tgttgtacac tctctaccgc
     4321 ttcttgtcgt tgccagccaa aataacggct gccgtcatat tcaataccta atgcgatttt
     4381 cattttaata ccttgataaa aagaggggcg aaaaattttt cgcccccaca gtaattgata
     4441 tttgcaatta tttattcaat aagcctaaaa attctttgcg ggtttctcgg tcttctaaga
     4501 aaacaccgcc aaaggcagaa gtaactgtat agctattggt gtcttttatg ccacggcatt
     4561 tcacgcaaaa atgggtggct ttcacataca ccgcaacatc gtctgtttct aaaatagttt
     4621 gaaaggcagt taaaatctgt tcggtaaaac gctcttgcac ctgcggacgt tgggcaaaaa
     4681 actgcacgac acggttgatt ttcgagagtc caatcaccca ttttttcggg taataggcta
     4741 ccgctacttt gccgtcaata gtaacaaaat gatgctcaca ggtagaagtt aaagtaatat
     4801 cgtccaccag caccatttca ctgactttca ttcggttttc aatattggta attttcggga
     4861 aggtagcata atcaagcccg ctgaaaattt catcaacata cattttcgcc aaacgatgag
     4921 gcgtttcttc taagctgtca tcacttaaat ctaaaccgag caattctaaa actgcctgca
     4981 tatgctgctg gatttctgct cggcgactgg ctttatcttt ggtatgagta acagtcggag
     5041 tttcaatgcc ttttgccaac aaggcttggc gaactttttg agcctccggc gaaagcgtgt
     5101 tttctgtgat agttaattcg ggcgagatcg ttgtcattgg ttttcctctc aaatgtaagc
     5161 tgaagattct aacaaattga catcaaaaaa aatagctcta aaatgagcta tttcagccat
     5221 aaacaccctc tttatttatg caaaatatac agtatctctt taatgccgac ctgattcccg
     5281 aacatcaagc tgaatttgtg cagtctctct taacttgcaa aaattttgac aattccgacc
     5341 gcttgctagg cttctcaaaa gggcgaggta cgacttggtt tttagatacc tctgctgaac
     5401 taggtatcaa tgtggtgctt cgccattatt atcgtggcgg cttgttcggt aaaatcatca
     5461 aagatagtta cttttttact cgctttgagg ccacccgagc gtataaagaa tttttcttac
     5521 tgcaacaaat gttagcttgg ggtttacctg tgcctcgccc tgtggcgatt aaaatagaaa
     5581 gacatttttg ttgctatcgt gccgacatta tgctcgaaaa gctagaaaac acacaagatt
     5641 taagcaaaat cttgcaatcc cattctttat cgaacgaaca atatgagcag atagggaaac
     5701 tgattcgcag tctgcataac catcaaattc accattccga cttaaatatc cataatattt
     5761 tgcttggtaa tgatggcaaa ttttgtctga ttgattttga taaatgcggc attgagccgg
     5821 gcgaaaattg gaagcaaaat aacctagacc gcctgttacg ctcgtttaag aaagaacaag
     5881 ggcgattaaa tattttcttc agcgaagaaa attggcaggc tgttttgaga ggatatactc
     5941 tctaagaaac aataatggca caagcttaga aacttgtgcc atcaaatcat caatattaac
     6001 tacttcactt cacgaatcat aatttcagac ggaatcacac tgccttgcca gtaaagttca
     6061 gaagctactt tttccgctaa tgcgaggtag gcttggctga tttcgtgttc cggagctgcc
     6121 accaccgttg gcacaccgct atctaaatct tcacgcagac gaatatgtag tggtaattgt
     6181 gctaacactt tagtgccata tttttgggcg attttttccg ctccgccggt gccgaaaatt
     6241 gcctcatggt gtccgcagtt tgagcagata tgcattgaca tattttcaat caccccaagc
     6301 actggtactg agactttctc aaacattgag atccctttaa tcgcatctaa taaggcaata
     6361 tcttgtgggg ttgttactac caccgcaccg gtcaccggaa tttgctgtga aagcgtaagc
     6421 tgaatatcac ccgtacccgg tggcatatca atgaccaagt aatccaattc attccaccaa
     6481 gtttcattta aaagctgact taacgcactg cttgccatcg ggccacgcca aatggtggcg
     6541 ttatcttctt ccattaaata gccgatagaa ttagattgca agccgtgagc ggtaatcggc
     6601 gtgatgtgct tgttatcagg agaagttgga cgttggtctt ttgctcccaa catatgcgga
     6661 atagatgggc cgtaaatatc cgcatctaaa atccccactc ttgtcccttg tgctcgtaag
     6721 gctaaagcaa gattaactga ggtcgttgat ttccctaccc caccttttcc tgaagtgacc
     6781 gcaatgatat tttttacccc attgactgcc gggtgtttat tcgcccgttt taaggtcgca
     6841 atttggtaat ttaataccca ttttacaccg cttgaacctg ttacttgctt taatttagcc
     6901 tctgtttcgg cttttaaggt ttcaaaacca ctgttccacg caaatggcat cgtgatttca
     6961 attctaagaa tatcgccccc taattctgct tttttaaacg cattaagggc aattaaatct
     7021 ttctgtaaag tcgggtggga aaattgttgc aagattgctt taatttcaac aagttgctgt
     7081 tcatttaatt ggttcattgg ttatccttgt ttgctaaatt gcgttttaaa tctttgctat
     7141 aaattacttc gctatcgtta ttcaaatcaa tacccaaaag ggaggaaaca atcccaaccc
     7201 gatgatggta ttgaaataat ggcaatacgg caacatcgct ataaagctgc tcgctaattt
     7261 cttgaataag tgactctctc gcttctgcac taagcgtttc tgtttgtaaa cgatttaatt
     7321 tttgatcgac tatctcactt ttatagccgg aatgattatc cgggctcatt gaatgaaact
     7381 tctgcaaaaa tacaatcgga tcaggataat ctgcacacca gcctgctcgt gctatctggt
     7441 aatctttttg ttcccttact ttatttaaag tctgccaatc aaccatttta ggtgtaatat
     7501 taaataattc tgattgagaa agggctcgag taacttggtt agcaatatta atgctattct
     7561 catcatataa caaagtcagc tttaaaggat tataagggct aattcctgct tttgttaata
     7621 gctgctcaac cacaacagga ttccaacgct tttctctatc ttttactaag ctatgtggaa
     7681 ttacagactg actcggaacc ccatattctt gcccactatt tgctgaagcc accattgtcc
     7741 ttattgcttc acgaatctct cgtttagcta ataatggatc atcaaaattg aactcatagt
     7801 aataagagca caaacgcgga aatttcacga tattcgtttt tttatttaat aaaggattct
     7861 cgacaatatc aaaagcagag cagttttgga tagttgtaat taaatcatac tctatagttt
     7921 gaaagggaat ttcttgggtt cgagccttta acaccatttt ttgagcatct agtttctcga
     7981 gacggtaaga cgcattgctc acgaagttat ctatggttgg agcaatccct tgataagtag
     8041 gtaataatgc ggaatgtgcc agcatttcag gcaattggtg atttgggtga tttagccgaa
     8101 ttctcaatgt atgcgcattt aatgcctcta caccgagttc ttcaactgac tttatttgct
     8161 cttcaatcgc ttttgcattt tctataccca tatagatgag atatttagac aatacagaat
     8221 gattttgagg atctattaaa cgtcgccagc ttgctacaaa atcttgtgca gtcacaggtt
     8281 ggccattaga ccacacagct ttctcatcta aaataaacaa ccactctttt tcatcttcag
     8341 taaaccaatt ctgagcaatg gccggaacga catcaccttt tctgttgtag gccgttaaac
     8401 cgactaatag atctcttaaa ggagcagcat ctgcagaagc tttaatttta tgtgggtcga
     8461 gttgaaatac agcagaataa attgcacgtt tgagtaaagt atcactctgc cggttttcta
     8521 cccagacaga ttgaatggat tgagggcaaa ttaattgatc acaagatgta ataagcagaa
     8581 tgcaagcggt aacttttgca atttttttta caaatgtaac cgcttgtaag gatttcaaca
     8641 taaactcccc aaaggttaat taagatttat tttacaagaa gcacaggaaa taaagttgta
     8701 attcctgcca caataatttc aattcccagc gccattaaaa tcagccccat gatacgagta
     8761 acgacattag atcctgtttt gcctaatttt ttcaccaaag gggctgaata gcggaataaa
     8821 atataacaaa ttaatgcaaa tacaataatc gcaatgctaa agccaacata gtcttcccaa
     8881 gtatggtagc gggttcccca cacaatggtt gagccaattg cccctggtcc tgccataatc
     8941 ggcattgcta atggcacgac accgatattt tcgtattcag taatatcttc atttttctct
     9001 tctttatttt gtttgtgttc acctaattta ccgctaatca tcgtcatcgc aatgcttaca
     9061 atcaaaatgc caccagctac tctaaaagag ctaagtgaaa tgctaaacgc attgagaatc
     9121 agattaccaa aatagaggct gactaataaa attactccaa tagaaacagc ggtgattaag
     9181 ctggtgcgat ttctttcccc ctcatattgg tgagcggtca tactgaaaaa aataggcaag
     9241 gaaccaaaag gattgactat agcaaataga ccaatgaaga attggaggta gatggcaaaa
     9301 ttaataatga tatccacaaa aactttctca aacaacagtg tttcgggcgc tattatatcg
     9361 gttaatcaag gtagatacaa accaaaagca cccagcctaa aggacggttg ggtgcttttt
     9421 gataaaatga aaggcttgtt agtgtgacga acgttgctgc ataaaatctt catttacctc
     9481 aattgaagct tttgcacgta gcgattgtaa gaattcatta cgtaaaatta aacgattagc
     9541 ttgttcaaat tgtggcgcca atgtcttaaa ctgttcagca ttcccttccg taatgttatc
     9601 taaggcaaca ataactacat cgcccttttg attacgtgcc atttgataag tcgttttatc
     9661 ttgcgccttt ttcattgcga aaatcgttcc agccaatacc gggttttcta cttctgcttg
     9721 catataaact aaagtttgtg gcgcattaaa tttcacgtcc actgatttcc cttcattcaa
     9781 ggcttttaca ttttcttcta ctttcgccag caacgctttt tccgcttttt ctgccttcac
     9841 aaaattttca actgctgctt ttgcttcatc aaaagtttgt ggacgttctg catcataatc
     9901 actcactctc acaaacaatg tctgtggatt tgcatcattc ccaacatcta aagcatcaga
     9961 attttgttta ttttggcgta gctctccttc aaacaaaact tttaatactt tctcattttg
    10021 taaagcagta ggtacgtttt cacgggtaaa cagatccgtt ttttgcagtt ccacattacc
    10081 ggctttcgca acttcttcaa gtgaagaatt attctcaaag gctttatttg ccatttcgcg
    10141 cgcaactgta aaatactcag ttaacactaa ttcttggcga atagtttggg caattttatc
    10201 tttcacttgt gctaactcaa tcacttgctc aggtttacgc tccaatactt tgatgatatg
    10261 gtaagcatta tccactttca ccgctgcact catctggccg gcagttaaat ttgctacagc
    10321 atcttcaaaa gctttcggga aagtgccagc ttttgcccaa cctagatcac caccattatt
    10381 tgctgataaa gtatcgctag atttgttttt tgccaaagta gcaaaatctt ccccattttt
    10441 gagcgcttgc tcaatagcct gtgcttccgc ttcactagcg acttgaatat gagctaaatg
    10501 ggcttcacct ttagttacat aatcggcttt attgcgatca tagtaagttt gaatttgctc
    10561 attggtaatc tcaactttgc tttccacatc ttttggagaa agagtaacgt actcaaccgc
    10621 taatttttca ggtgctaata atttagtttt atttgcttcg taataggctc gcaattcttg
    10681 ctcggaagcg gtctgatttt ccatttcttt tgcaactgaa aattcggcta aacgcacttc
    10741 acgttgttgc aacagtaatt ttgctaacaa tgcctgctgt gccggtacat taaaatcact
    10801 attgataatc ccctcttgta gctgggattg caacatacct tcctgcacaa tcgctgcata
    10861 gccatcggca cttaaattat tgtggcgtaa tgtttgttgg tataactcgt tactgaattt
    10921 accatcttgt tgaaacatct ggctgtttac aatttcagat ttgatttgat cagcactaat
    10981 acctagtttt aactcttttg catactggcg cagaagctct tcatcaatta atccatttaa
    11041 taccgattga ttaaattgag cagcatactc cgggctatcc attaaatccc aaaagcgact
    11101 ccccagttca gcatttaatc tattttgctc acgattaagc gtgttattaa ataattgctg
    11161 actaatttct tccccattta ctttcgctac agaattatct tgtgaaatca atcctgtccc
    11221 aattccaccg attacaaatg aaatagagac taatgcaaaa ataattttaa agaccggccc
    11281 atttgtccgc tcgtgcattt tttca
//
LOCUS       DANLXF010000067        11208 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.067, whole genome shotgun sequence.
ACCESSION   DANLXF010000067 DANLXF010000000
VERSION     DANLXF010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11208
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.067"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            208..936
                     /gene="ttrB"
                     /locus_tag="PXD66_001155"
     CDS             208..936
                     /gene="ttrB"
                     /locus_tag="PXD66_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetrathionate reductase subunit TtrB"
                     /protein_id="HDL6319465.1"
                     /translation="MDLSKRSFLKELSALTVGASFVPLQSAQAFMPAWREGDENKRYA
                     MLIDLRKCIGCQACTVSCSVENATPLHSFRTTVRQYEITNGTQVVNNVVLPRLCNHCD
                     QPPCVPVCPVQATFQRKDGVVVINNEQCIGCGYCVQACPYDARFINEETKTADKCTFC
                     THRLEVGLLPACVESCVGGARVIGDLNDPKSQISQLYQAHKDDLKVLKPEAGTVPHVF
                     YVGLDDDAFVSKIDGQPMLWTGEA"
     gene            938..1969
                     /gene="nrfD"
                     /locus_tag="PXD66_001156"
     CDS             938..1969
                     /gene="nrfD"
                     /locus_tag="PXD66_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HDL6319466.1"
                     /translation="MIREVLVEPQHIVWLPWIVHYFFFVGVAATAVFTAVLFAKKSLK
                     PTACELAAVTVALIGSIVAPVALTADLHQPSRILHFYTDFAWRSPMAWGAIILPLFSV
                     AVAGYFVLALAHHTRPNLPKWLAWLQLPILQNQGLLWAFRLFATLTAIGIIGYTVLET
                     YQTGTRTLWHSAWLLPIMLFSAWAVALGLTQAVCKMLFHTQTNTGLWTKLCLILTALS
                     LVGLAFSSPQATQDFAQLFNGSITAYLVGIFWLIALICNFSAKNHRLQWLGVLALIAF
                     GWLLRWVLVIQVQTIAKTNALQNPYHFDWTAVDGGLGIVSIFGLTVLVSIVVWQLILI
                     ICGGRKGEQ"
     gene            1959..5054
                     /gene="ttrA"
                     /locus_tag="PXD66_001157"
     CDS             1959..5054
                     /gene="ttrA"
                     /locus_tag="PXD66_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetrathionate reductase subunit TtrA"
                     /protein_id="HDL6319467.1"
                     /translation="MNNKRRNLLKGGLALGGAAAFGAGYSPKVSEIAKGVMHGTSGEK
                     TRDAIHGNSLTPEYQVKDGKLLSNPEQIVCNTQCMGCWTQCGIRARVDLNTNQVIRIS
                     GNPYHPLSSDKTLPFGMPIAKAELLLAGESGIENRSTACARGAAFLEGVNSPYRITQP
                     LKRVGKRGEGKWQTISFEQLVEEIINGGNLFGEGEVEGLKTIRNLVDPINPKQPDLGM
                     KANQLLVTFAGPEGRQPILQRFAQKSFGTINFSNHGAYCGAAYRAGSGAFMKDFDNQQ
                     HAKPDWDHAEFILFIGTSPAQSGNPFKRQARQLAKRRPDDNFSYVVVSPRLEMTSSLA
                     TQNNHWVPIKPEGDLALVMGMLRWIIENERFNADYLSRPSLAAMENAGHVSYANASHL
                     IICEPNHPKFGQALRISDLQDVQLDPEEKVEENLIVVKDQASGELISAKDCLQAVIFV
                     EQIAKLLDGSEVLVKSSMQLLKEATQEKTLAEYSDICGVPVQTIENLAKKFTSHGHKA
                     NAITHGGTMHSTGFYTSWAILLLNAMVGNLNKKGGMAVAAGKFKDFGDGPRYNLANFP
                     NEVKPKGTNLARSKKAYQNSSEFKQKVTKGENPYPAQGAWYPFTGGQTTEMFTSMVQG
                     YPYFAKAWINHMANPVYGLTAMNPIALEKLKDPKILPLFVSIDAFMNETTALTDYIVP
                     DTHNFESWGFSTAWGGVPQRTSTARHPIVPSKNAKTAEGDTICMENFVIAVAKKMGLP
                     GFGENAIADLEGNRYPLHRTEDYFLRAAANIAFAGEKPAPDASEQDLLLTGVQRIMPK
                     IEQTLKAEERLKVANVYCKGGRFAPHESAWKEENMEFQWKNCLQIWNENVYKAQHHSN
                     GEHYWGCPRYMSPRFADGSTLEQHYPQSEWGFKLISFKSNIMSSITAPLLRLLSIKPE
                     GLVAMNRLDAEEKGIKHGDVVKLSTPTAGLTVQIVVLDGIARGTIGIEHGYGHKQIGA
                     SSYTIDGVEIPANPMIAKGINLNDLGIIDHTKAVKSPWVDWVCGSAVRNGIPAKVEKL
                     A"
     gene            5195..6040
                     /locus_tag="PXD66_001158"
     CDS             5195..6040
                     /locus_tag="PXD66_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL6319468.1"
                     /translation="MTFVKKTALSLIATALFATSSFAAEQSQYQHIRNATAKIEYAGK
                     TFLVDPYLAEKDRYLPPNAKKRNPTLDMTVSIDEVLNGVDAVIVTHTHHAHWDDVAQQ
                     RVPKNLPIFVQNAGDARLIRSQGFTDVRVVGKDTPFEGVTLTRTRGQHGTDAMYANPK
                     IAEMVGDAMDVVMKAEGKPTVYLVGDTIWNHEIEHSFNTYKPDFVVLNAGCAKLPNFE
                     GSPIMCTEDVSKAHKIAPQAKILTVHMDALPQTSISSDQMRDYVQKEGLNRVTVPKNG
                     DVIKF"
     gene            complement(6153..7550)
                     /locus_tag="PXD66_001159"
     CDS             complement(6153..7550)
                     /locus_tag="PXD66_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HDL6319469.1"
                     /translation="MKLSKLAFSVALALTVTACTTGNMSNDGTLQQAQQTYQSYDEIT
                     KQYQINEQWWQGYTDAELNRVVDLAIKNNLDLAKSGIAVNKALYNANLVGANLVPTFS
                     GSGSVSAAKGLGSASSNNVSTGVSTTAAQAAFNLSYTVDLWRRLADTASAAEWTHKAT
                     IEDLKATRLSIINAVVTTYYQLAYYRDAIRITEESIRHYEQISRILTNKMNAGAIDSL
                     AVDQAQQAVLNARNSVIDLKTNQKIAEQTLRNLLNVQPNTPLNIRYPNIMSVKLQGVD
                     LNVPVSAIANRPDVVGSLQRLQSAFKTLTATEKSWFPTLTIGGSITGSATNIGNVADN
                     PVGNGLIKLDLPFLDWNRVKNNVKISETDYVTARLNYEQKITSALNEIDRYYYSYTQS
                     RSSLKILQDTNAKNKRISSYYKNRYEQGVSEFREWITALNTELNSQLAILNAKYAILT
                     NENAVYQAMAGKYRR"
     gene            complement(7640..8263)
                     /gene="gmk"
                     /locus_tag="PXD66_001160"
     CDS             complement(7640..8263)
                     /gene="gmk"
                     /locus_tag="PXD66_001160"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDL6319470.1"
                     /translation="MSLGNLYIISAPSGAGKSSLINALLADLPRSEVQLSISHTTRQP
                     RPAEQDGVHYYFTSHDEFKQLIEQGYFLEWAEVFGNYYGTSLPMIERSLEQGIDVFLD
                     IDWQGARQIREKVPNVKTVFILPPSKTELEKRLIGRGQDSAETIAKRMAEAVSEMNHY
                     NEFDYVIINDDFQQALAELKAILIAERLSLGSQQIRHQHLITDLLNN"
     gene            complement(8376..8849)
                     /gene="gpt"
                     /locus_tag="PXD66_001161"
     CDS             complement(8376..8849)
                     /gene="gpt"
                     /locus_tag="PXD66_001161"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDL6319471.1"
                     /translation="MSEKYTNEKYVVTWDMFHMHARKLAENLLPATQWKGIIAVSRGG
                     LFPAAVLARELGIRHVETVCIASYDHDSQGELNVLHAADTDGEGFIVVDDLVDTGNTA
                     KEIRRMYPKARFVTVFAKPHGAPLVDDYVIDIPQETWIEQPWDLGITFVQPLARR"
     gene            complement(8995..10404)
                     /locus_tag="PXD66_001162"
     CDS             complement(8995..10404)
                     /locus_tag="PXD66_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL6319472.1"
                     /translation="MKKYPLLFSLFSPVVWAEKVIEHNTDPISFERQPQAVKFEQNVV
                     KNSQKPTAYQPAYSVNQNVEQQINQAILSQDWATLEPLLSRYPQTENFDPLLWHYAQA
                     ALAYAKKDHQTAVFHYRQLLAKPPSLVYPRFDLALILAEDQQFRAAEQEFKAIQAHLP
                     PELAKIARNTQVQIAENEAWQPDFYLQYTQTDNVNNASSSPIVNVNGRIFRKNADSLP
                     QSAKGVRYGLGLSKLHNVKGNHFVGLTLNYSGIYYWDNRDYREQSVSVSPNYSYRTVA
                     YRLTFSPFIEQNWLGSSRYNVQFGATLNGLRYLNSQWLVSGSFTHLQKRYFEELTASR
                     YNSYQHQLSGGVQWQAVKNGRFFANLDGVREIAREKAQSSNKWLARVGAVFQGERWAT
                     QASIGFGKRYFADKHYLFGYKRLDTEYQANLAVWNRTWHWQGIVPKLNFRYQKIDSNI
                     ADFYSRQNKEVFFTLEKLF"
     gene            complement(10600..>11208)
                     /locus_tag="PXD66_001163"
     CDS             complement(10600..>11208)
                     /locus_tag="PXD66_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HDL6319473.1"
                     /translation="AEKEVDPIAIIKAKGIDDTNYGLKLGNSKETIEGKVTVRTQSYL
                     YNQPYSVVTAQAVQRNGWENGNYIDKVDGTVTVKGLKTEKLPTEGTATYSGKAFNHDG
                     AKGFNGGDLTYNVNFSKRTGSGRVEGEFGNLIVLEEGTINNTQISSTVHGNNPLNLSG
                     KYTVEFFGKNAEEIAGEIQTKANLTGEVSNFGLAGTRGDIQK"
BASE COUNT         3020 a         2617 c         2572 g         2999 t
ORIGIN      
        1 taatgcgtat aaaaacttta gatctaaact aaaccatctt attgcttttt aattagtatc
       61 aatgccctat tatctacttg tatagggcat ttcccttcat ccttgaccca aatcaacttt
      121 ccccttgata tgtggaaaac cacaatacct tgccgttccc tctccgcctt agaatacctt
      181 tgttattttt aaatttgtgg gggaaatatg gatttaagca aacgttcctt tttgaaagag
      241 ctttccgcct tgacggtggg ggcgagtttt gtgccgttgc aatcggccca ggcgtttatg
      301 ccggcttggc gtgagggcga tgagaacaaa cgctatgcga tgctgattga tttgcgtaaa
      361 tgtatcggct gtcaagcgtg taccgtgagc tgttcggtgg agaacgccac gccgttgcac
      421 agtttccgca ccacggtgcg tcaatatgaa atcaccaacg gcacgcaggt ggtgaacaat
      481 gtggtgttgc ctcgtttgtg taaccattgc gatcaaccgc cgtgtgtgcc ggtctgcccg
      541 gtgcaggcaa cctttcaacg taaagacggt gtggtggtga ttaacaatga gcagtgcatc
      601 ggctgtggtt actgcgtgca agcctgcccg tatgatgccc gttttatcaa tgaagagacc
      661 aaaaccgccg ataaatgcac cttctgcact caccgtttgg aggtaggtct gctgccggcg
      721 tgcgtggaaa gttgcgtggg cggggcaagg gtgattggcg atttgaacga cccgaaaagt
      781 caaatttccc aactttatca agcccacaaa gatgatttga aagtcctgaa accggaggcc
      841 ggcaccgtgc cacacgtttt ctatgtgggg ctagacgatg atgcctttgt gagcaaaatt
      901 gacgggcaac caatgctctg gacgggggag gcgtaaaatg attcgtgaag tattagtgga
      961 accgcaacat atcgtctggt tgccgtggat tgtgcattat ttcttcttcg taggcgtggc
     1021 ggcaacggca gtgtttaccg ccgtgctgtt tgcaaaaaaa tcactgaaac cgaccgcttg
     1081 cgagctggcg gcggtgacgg tagccttgat tggctcgatt gtcgcccctg tggcattgac
     1141 tgccgacctg caccaaccga gccgtattct gcatttctac accgattttg cgtggcgatc
     1201 tccaatggcg tggggagcga ttatcctgcc cctgttcagc gtggcggtag cgggttattt
     1261 tgtgctggca ttggctcacc atacccggcc gaacctgccg aaatggttag cgtggctaca
     1321 gttgccgata ctgcaaaatc aaggcttgct ttgggcattc cgcctgtttg ccacactcac
     1381 cgctatcggc attatcggct acaccgtgct tgaaacctac caaaccggca caagaacctt
     1441 atggcacagt gcgtggctgc tgccgattat gctcttttct gcgtgggcgg tggcattggg
     1501 gcttacccaa gcggtctgca aaatgctgtt ccacacccaa accaacacgg ggctatggac
     1561 aaaactctgc ctgattttaa ccgcactttc gcttgtcgga ctagcattca gtagtccaca
     1621 agccacacaa gattttgccc agctcttcaa tggctcaatc accgcttacc ttgtcggcat
     1681 tttctggcta attgccctca tttgcaattt ttctgccaaa aaccaccgct tgcaatggct
     1741 cggcgtgctt gccctcatcg ctttcggctg gctactccgc tgggtactgg tgatccaagt
     1801 ccaaaccatt gccaaaacca atgccttgca aaatccatat catttcgatt ggacagccgt
     1861 cgatggtggt ttggggattg tctcgatttt tgggctaacg gtgctagtaa gtatcgtggt
     1921 atggcaactg attttaataa tttgtggggg aagaaaaggt gaacaataaa cgcagaaatc
     1981 tgttgaaagg cgggctggca cttggtgggg cggcggcgtt tggggccggg tattcgccga
     2041 aggtgtcgga gattgccaaa ggggtgatgc acggcacatc gggcgagaaa acccgtgatg
     2101 caattcacgg taattcgctc acgccggagt atcaggtaaa agacggcaaa ctgctcagca
     2161 atcctgagca aattgtgtgc aatacacagt gtatgggctg ttggacgcag tgtggcattc
     2221 gggcgagggt ggatttgaac accaaccaag tgattcgcat ttcgggcaat ccgtatcacc
     2281 cgctttcctc cgacaaaacc ctgccgttcg gtatgccgat tgccaaggcg gaactgttgc
     2341 tggcgggcga aagtggcatt gaaaatcgct ccaccgcctg tgctagaggg gcggcgttct
     2401 tggagggggt gaacagcccg tatcggatta cccaaccgtt gaaacgggtc ggaaaacggg
     2461 gcgaaggcaa gtggcagacc atcagctttg agcagttggt ggaggaaatc atcaatggtg
     2521 gcaacctatt tggtgaaggc gaagtggagg gcttgaagac gatccgcaat ctggtcgatc
     2581 cgattaaccc gaaacagcct gatttgggaa tgaaagcaaa ccagctttta gttacttttg
     2641 ccggccccga aggtcgtcag ccgattttgc aacgctttgc ccaaaagagc ttcggcacga
     2701 tcaacttttc caaccacggg gcttattgtg gcgctgcgta ccgtgccggt tcgggtgcct
     2761 ttatgaaaga ttttgacaac caacaacacg ccaagccgga ctgggatcac gctgaattta
     2821 ttctgtttat cggcacgtct ccggcacagt cgggcaatcc gtttaagcgt caggctcgcc
     2881 agcttgccaa acgccgccct gatgacaatt tcagctatgt ggtggtttcc cctcgtttgg
     2941 agatgacctc ctccctcgcc acccaaaaca accattgggt gccgattaag cccgagggcg
     3001 atttggcgtt ggtaatggga atgttgcgtt ggattatcga aaacgagcgt ttcaatgctg
     3061 actacctctc ccgcccaagt ctggcagcga tggaaaatgc cggccacgtc agctatgcca
     3121 atgcgtcaca tctgattatt tgcgaaccga accacccgaa attcgggcag gcgttgcgga
     3181 ttagcgatct gcaagatgtg caactcgacc ccgaagagaa agtggaagaa aacctgattg
     3241 tggtaaaaga tcaggcaagc ggcgaactca tttcagcaaa agattgctta caagcggtca
     3301 tttttgtcga acaaattgca aaactgcttg atggctcgga agtgttggtg aaatcctcaa
     3361 tgcagttgct gaaagaggcg acacaagaga aaacccttgc cgaatatagc gacatttgtg
     3421 gcgtgccggt gcaaaccatt gaaaacttag cgaaaaaatt cacttcgcac ggtcataaag
     3481 ccaatgccat cactcacggc ggcacgatgc acagcaccgg tttttatacc agttgggcga
     3541 ttttgttgct gaatgcaatg gtcggtaacc tcaacaaaaa aggcggaatg gcagtagccg
     3601 caggcaaatt caaagatttc ggggacgggc ctcgctacaa tttagcgaac ttcccgaatg
     3661 aagtgaagcc aaaaggcacg aaccttgccc gcagtaaaaa ggcgtaccaa aattcaagcg
     3721 aatttaagca gaaagtgacg aagggcgaaa atccgtatcc ggcacaaggg gcgtggtatc
     3781 cgttcaccgg cgggcaaact accgagatgt ttacctcaat ggtgcagggc tatccatact
     3841 ttgcgaaagc gtggatcaac catatggcga accccgttta cggcttaacc gctatgaacc
     3901 cgattgcgtt ggagaaatta aaagatccga aaattctacc gctctttgtc tctattgatg
     3961 cttttatgaa cgaaaccact gcactgacgg actacatcgt gccggacacc cacaactttg
     4021 agagctgggg cttttcaacg gcgtggggcg gtgtgcctca acgcaccagc accgcacgcc
     4081 acccgattgt gccgtccaaa aacgccaaaa ctgccgaagg cgacaccatc tgtatggaga
     4141 atttcgtgat tgcggtggcg aaaaaaatgg gcttgccggg ctttggcgaa aatgcgattg
     4201 ccgatttaga aggcaaccgc tatccgctcc accgtaccga agattacttc ctacgggcgg
     4261 cagcgaacat tgcctttgcc ggtgagaaac ctgcaccgga cgccagcgag caagatctgc
     4321 tgctcaccgg cgtgcaacgg attatgccga aaattgaaca aacgctaaaa gccgaagagc
     4381 gattaaaagt ggcgaatgtg tattgcaaag gcggacgttt tgccccacac gaaagtgcgt
     4441 ggaaagagga gaatatggag ttccagtgga aaaactgcct gcaaatttgg aacgaaaacg
     4501 tgtataaagc ccagcaccac agcaacggtg aacactattg gggctgccct cgttatatgt
     4561 cgccacgttt tgcggacggc tccacgctgg agcagcacta tccgcagtcg gaatggggct
     4621 ttaaactgat ttcgtttaaa tcgaacatta tgagcagtat taccgcccca ctgttgcgtc
     4681 tgctctcgat caagcctgag ggcttagtgg cgatgaaccg tttagatgcg gaagaaaaag
     4741 gcattaaaca cggcgatgtg gtgaaactca gcaccccaac cgccggcttg acggtgcaga
     4801 ttgtggtgtt ggacggtatc gcccgaggca caatcggcat cgaacacggc tacggtcata
     4861 aacagattgg ggcaagttcc tacaccattg acggcgtgga aattccagcc aacccgatga
     4921 tcgccaaagg gattaacctc aacgacctcg gcattatcga ccacaccaaa gcggtcaaat
     4981 ccccgtgggt agattgggtc tgtggctcag cggtgagaaa tggcattccg gcgaaggtag
     5041 agaaattagc ctagccgaac aagcggttgg ttttgcagaa aaatgtgcaa aatcgaccgc
     5101 ttttattgat gtaagggaca ctgttcctta tgcaaaccag cattgcagag cgtaaaaaat
     5161 catactctgc attttaattt attttggaga acctatgact tttgtcaaaa aaacagcact
     5221 gagcctgatt gcaacggcac ttttcgctac atcaagtttt gccgctgagc agagccaata
     5281 ccaacatatt cgtaatgcaa cggcgaaaat tgaatatgcc ggcaagacgt ttttggttga
     5341 tccgtattta gcggagaaag atcgctactt accgccgaat gcgaaaaaac gtaatccgac
     5401 cttggatatg actgtgagca ttgatgaagt gttaaatggc gtggatgcgg tgatcgtaac
     5461 tcacactcac cacgctcact gggacgatgt agcacaacaa cgtgtgccga aaaatctgcc
     5521 gatttttgtg cagaatgccg gtgatgcccg tttaatccgt tcacaaggct ttaccgatgt
     5581 gcgtgtggtc ggcaaagata ccccgtttga aggcgtaacc ttaacccgaa ctcgtggcca
     5641 gcacggcacc gatgcgatgt atgcgaaccc gaaaattgcc gaaatggtcg gcgatgcgat
     5701 ggacgtagtg atgaaagcgg aaggcaaacc gaccgtctat ttggtgggag ataccatttg
     5761 gaaccacgaa atcgaacatt cgttcaacac ttacaaacca gactttgtgg tgctgaatgc
     5821 cggctgtgcc aaactaccaa actttgaagg cagcccgatt atgtgtaccg aagatgtttc
     5881 caaagcacac aaaatcgctc cacaagcgaa aatcttaact gtgcatatgg acgccctgcc
     5941 acaaacctca atcagcagtg atcagatgcg tgattatgta caaaaagagg gattaaatcg
     6001 tgtaactgtg ccgaaaaacg gtgatgtgat taagttctag tagctaaact aacattacca
     6061 aaatggttta agtagcaata aatagcgaag cgggttaagc catcaagcct taacccactt
     6121 ttaccaacca tagtatatag ttttagatat aattatcttc tgtatttacc tgccattgct
     6181 tggtaaaccg cattctcgtt ggtcaaaatc gcatattttg catttaaaat cgccaactga
     6241 gaattaagct cggtattcag tgcggtaatc cactcacgga actccgacac cccttgttca
     6301 taacggtttt tatagtatga actgatacgt ttatttttcg cattggtatc ttgaaggatt
     6361 tttaacgagc ttctcgactg cgtataactg taataataac ggtcaatttc atttaaggct
     6421 gaggtaattt tttgctcata gtttaaacgg gcagtcacat aatccgtttc agaaattttt
     6481 acgttatttt taacccgatt ccaatctaag aacggtaaat ccaatttaat taagccgtta
     6541 cccacagggt tatctgccac attgccgata ttggttgcac taccggtaat agaaccaccg
     6601 atggttaagg ttgggaacca gcttttctcg gttgcggtta aggttttaaa tgcactttgt
     6661 aagcgttgta acgagcctac tacatcaggg cgattagcaa tggctgacac tggcacattt
     6721 aaatctaccc cttgcaattt cacgctcata atattcgggt aacgaatgtt aagcggagta
     6781 tttggctgaa catttaacaa attgcgtaat gtttgctcgg caattttttg gttggttttc
     6841 agatcaatta cactatttct ggcgtttaag accgcttgtt gagcctgatc aaccgctaaa
     6901 ctatcaatcg caccggcatt cattttgtta gttaaaatac ggctgatttg ctcataatgg
     6961 cgaatgctct cttcggtaat acgaatcgca tcacgatagt aagctaactg gtagtaagta
     7021 gtcacaaccg cattaataat agataaacgg gtagctttta agtcttcaat ggtggcttta
     7081 tgcgtccatt ctgccgcact ggcggtatcg gctaaacgtc gccataaatc cacagtataa
     7141 cttaaattaa atgcagcttg agctgcggtt gttgaaacac cggtcgaaac attatttgaa
     7201 gaagccgaac ctaaaccttt agccgcagaa acagaacctg aaccactaaa ggttggcact
     7261 aaatttgccc ctactaaatt agcgttatac aaggctttat tgactgcaat tccgcttttg
     7321 gctaaatcaa ggttattttt gatggctaaa tcaaccacac ggtttaattc cgcatccgta
     7381 tagccttgcc accattgctc gttaatttga tattgtttgg taatttcatc atagctttga
     7441 taggtttgct gtgcttgttg caaagtcccg tcattgctca tattccctgt ggtacaagcc
     7501 gttacggtta gagccaacgc cacagaaaat gctaattttg aaagtttcat tacatttcct
     7561 tttgaaaata aagaggggaa aatgggcgta ttctacccaa aaacagattg cctttccagt
     7621 taaatgaaca actgttcatt taattattta acaaatcagt aatcaaatgc tgatgacgaa
     7681 tttgttgcga acctaagctc aaacgctcgg caattaaaat cgcttttagc tctgccaatg
     7741 cctgttggaa atcatcatta ataatcacat aatcgaactc attgtaatgg ttcatttccg
     7801 acaccgcctc tgccatacgt ttggcaatgg tttcagcgga atcttgccca cgcccgatta
     7861 aacgtttttc caattctgtt tttgacggtg gcaaaataaa aacggttttc acattcggca
     7921 ctttttcccg aatttgtcta gccccttgcc agtcaatatc caaaaagaca tcaatgccct
     7981 gctctaggct acgttcaatc atcggcaatg atgtgccgta ataattgcca aacacctccg
     8041 cccattctaa aaaataccct tgctcaatca gctgcttaaa ctcatcgtgc gaagtgaaat
     8101 aataatgcac gccgtcctgt tctgccggac gaggctgacg ggtagtgtgt gaaatcgaaa
     8161 gctgtacttc cgaacggggt aaatccgcca acaaggcgtt aattaaggaa gattttcctg
     8221 ctccacttgg ggcagaaata atataaagat tgcctaaact cattatttgt atatacctat
     8281 ttgattttgg gaatagggcg aatgatgatt cgcccctaca tttcatagga ataaataaaa
     8341 aataatggac aactttcatt gtccattaca caaaattatc tacgtgccaa aggctgaacg
     8401 aaggtaatgc ctaaatccca aggctgctca atccacgttt cttgtggaat atcaatcaca
     8461 taatcatcaa ccaacggtgc accgtgcggt ttcgcaaaca cggttacaaa acgagctttc
     8521 gggtacattc tgcgaatttc ttttgccgtg ttgccggtat ccactaaatc atctaccaca
     8581 ataaagcctt cgccatcggt atcggctgcg tgtaacacat ttaactcacc ttggctatcg
     8641 tgatcgtagc tggcaatgca cactgtttct acgtggcgaa tacccaactc acgtgctaaa
     8701 acggctgccg gaaacaagcc gccacggctc accgcaataa tccctttcca ctgtgtggca
     8761 ggtaataaat tttctgccaa cttgcgagcg tgcatatgga acatatccca cgtcacgaca
     8821 tatttttcat ttgtgtattt ttcactcata gaaacaacct aatataaaat tgggggctac
     8881 cccgatataa aaaattgcac taggataacc cgaaaatgct tttttcgcca tcatttcatt
     8941 tatttttttg tgccggcagc tacgcaatcg tatgcttagc acaaaaaacc aacactaaaa
     9001 tagcttttct aaagtgaaaa agacttcctt gttttggcga gagtaaaaat cagcaatatt
     9061 gctgtcaatt ttttgataac ggaaattcag cttcggcaca atgccctgcc aatgccaagt
     9121 gcggttccaa accgctaaat tggcttgata ttccgtgtct aaccgcttat agccaaacaa
     9181 atagtgcttg tcggcaaaat atcgcttgcc aaagccgata cttgcctgcg ttgcccagcg
     9241 ttcgccttga aaaaccgccc ccactcgagc cagccactta ttcgagcttt gtgctttttc
     9301 ccgtgcaatt tcacgcacgc cgtctaaatt tgcaaaaaac cgcccatttt tgaccgcttg
     9361 ccactgcaca ccgccactga gttggtgctg atagctgtta taacggcttg cggttagctc
     9421 ctcaaaatac cgtttttgca aatgggtaaa actccccgac accaaccatt gcgaattaag
     9481 gtaacgcaaa ccgttgagtg ttgccccaaa ttgaacatta taacggctac tccccagcca
     9541 attttgctca ataaaaggcg aaaaagtcag ccgataagcg acggtgcggt agctataatt
     9601 cggcgaaacg gatacggatt gctcacggta atctcggtta tcccaatagt aaatcccgct
     9661 ataattgagc gttaaaccga caaaatgatt gcctttcacg ttgtgcaatt tactcaaccc
     9721 taagccataa cgcacgcctt ttgctgattg tggcaaactg tctgcatttt tgcggaaaat
     9781 ccgcccattt acattcacaa tcggcgaaga tgaggcatta ttcacgttat ctgtttgggt
     9841 atattgcaga taaaaatcgg gctgccacgc ctcattttca gcaatttgga cttgagtatt
     9901 gcgggcaatt ttagcaagtt ccggcggtaa atgggcttga atagccttaa attcctgctc
     9961 tgccgcacga aattgctgat cttctgccaa aatcaacgcc aaatcaaaac gagggtaaac
    10021 caaactcggg ggcttagcta acagttggcg atagtgaaaa actgcggttt gatgatcttt
    10081 tttcgcatag gcaagtgcgg cttgcgcata gtgccacaac aacgggtcaa aattttcagt
    10141 ttgcggataa cggcttaaaa gcggttcaag cgttgcccaa tcttggctga gaatcgcttg
    10201 gttaatttgc tgttcaacat tctgattgac tgaatacgca ggctgataag cggtcggttt
    10261 ttgtgaattt tttacaacat tttgctcaaa tttaaccgct tgcggctgac gctcaaagga
    10321 aatcgggtca gtgttgtgtt caatcacctt ttctgcccat acaacggggc tgaacagcga
    10381 aaataaaagg ggatattttt tcataaagat aaagaaaatt ggtaggggcg gacctatatg
    10441 ttcgcctcta cgggatttat cactctctcc ccttgtggga gagggttatt agaagctgta
    10501 aattgaaatc taaactattg tgtcttatgg tatctacaag caatatcaag acaaatgccg
    10561 tttaattttt cgttaaacgg cattgaacgt tagaaaaggt tatttttgga tatcgccacg
    10621 agtgccggca agtccaaaat tactcacttc cccagtaaga tttgcttttg tctgaatttc
    10681 accggcaatt tcctctgcat ttttaccgaa aaattcgact gtatattttc cagaaaggtt
    10741 taaaggatta ttgccatgta ctgttgaaga aatctgagta ttattaatcg tcccctcttc
    10801 taatacgatt aagtttccaa attctccctc aactctacct gaaccggtac gcttagagaa
    10861 attaacatta taggttagat caccaccatt aaaaccttta gctccatcgt ggttaaatgc
    10921 ttttcctgaa tatgttgcag taccctctgt tggtaatttc tctgttttca atcctttaac
    10981 tgtaactgtt ccatcaactt tatcaatata attaccgttt tcccagccat ttctttgaac
    11041 tgcttgtgct gttactacag aatatggctg attatataaa taactctgtg ttctgactgt
    11101 gactttaccc tcaatagttt ctttactatt accaagtttt aagccgtaat ttgtatcatc
    11161 aatacctttt gctttaataa tagctatagg atctacttct ttttctgc
//
LOCUS       DANLXF010000068        11180 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.068, whole genome shotgun sequence.
ACCESSION   DANLXF010000068 DANLXF010000000
VERSION     DANLXF010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11180
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.068"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(74..3016)
                     /gene="rne"
                     /locus_tag="PXD66_001164"
     CDS             complement(74..3016)
                     /gene="rne"
                     /locus_tag="PXD66_001164"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HDL6319474.1"
                     /translation="MKRMLINATQKEELRVALVDGQRLFDLDIESPGHEQKKSNIYKG
                     KITRVEPSLEAAFVDYGAERHGFLPLKEISREYFPSDYVFNGRPNIKDIIQEGQEVIV
                     QVSKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDERLELKEALDCLDV
                     PEDVGLIVRTAGVGKSPEELQWDLKVLLHHWEAIKKAAESRPAPFLIHQESDVIVRAI
                     RDYLRRDIGEILIDNKKVFEKAKNHIRLVRPDFINRVKLYEGEVPLFSHYQIESQIES
                     AFQREVRLPSGGSIVIDVTEALTAIDINSSRSTRGGDIEETALNTNLEAADEIARQLR
                     LRDLGGLIVIDFIDMTPVRHQREVENRIREATRQDRARIQFARISRFGLLEMSRQRLS
                     PSLSEASSHICPRCQGTGKVRDNESIALSILRLLEEEAIKENTAQVHTIVPVQIASYL
                     LNEKRKAISSIEKRHDVQIVVVPSESMETPHFSVYRLRENEITPTLSYDLTKHYEARH
                     EEEHPHHHHQEDVLVTRNEPVITVESVLQSSELNLQPSPTPTEPPKPSLLSRLIATIK
                     GWFSEEKVEEKPKPKARNQRERRQRRERPNRNAKAENQERKTPQAQTTPAEEKPMRSE
                     KKARRAVVEEVSGVTEQESRSNSREVQTERRQRRDLRKKVRIETQEVEVENLTPAVEE
                     TVAKQVENVQKNQQIQPLATQEVPVETAPEVETTNEGRENNRQRRLPRHLRVGNQRRR
                     ENQRNQVISPMPLTSAVASPELASGKVWVQVTSTVKVEEKASFLSVDQMLEQQENQLV
                     ETEKKSEEKKLHTVSPLGGFVAQATNRDLVKEKQEQLSKPKEEKVASEDVKPVEVKEH
                     IAPFGSVVAQHSERDLVKEKNERVSKFQSQATVAPAIVEEVTNPIEVPKSTYESDYRF
                     NGRLGTFSRVEHTQAAMTIASAPSDKLEVVEISQWQNSKYYFYGKGFGGYTSAVSQVQ
                     SAPRSTNG"
     gene            complement(3473..4318)
                     /locus_tag="PXD66_001165"
     CDS             complement(3473..4318)
                     /locus_tag="PXD66_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL6319475.1"
                     /translation="MMSQEQFEEINQERRQALNHGSRIAMAASVLGALGIASTANAKQ
                     ASAEKSPYAEPEQYGLKRHGMTIDPKRVALVVVDPQIDFLGPKGVMWGVLGDSIKENN
                     TVANIESLFKAAKAVDMPTFVSPHYYYPTDHKWEFGSPGEHFMHDSGMFARKGQYTME
                     GFENSGADFMPEYKPYIFDGKTVITSPHKIFGPETNDLVLQLRKRKIDQVILAGMAAN
                     LCIDSHLRELIEQGFEVTVVKDATAGPRIPEGDGYLAALTNYRIIANDLWTTEEAVKK
                     MTAKA"
     gene            4445..5269
                     /locus_tag="PXD66_001166"
     CDS             4445..5269
                     /locus_tag="PXD66_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL6319476.1"
                     /translation="MDSLTHLFTQFQFRTDLFHLGQLCQIGSFNEENKGYLHFVRQGR
                     FILNQAAERPITIERPAIIFSPTNILHSLHPLDDSGLDIFCINFDFGKGIRNPLTHTL
                     HNIVILELAQYPELGVLADQIFSEVNQKGCGYQAMIHHLCAYFTLKVARQCLEKGYIQ
                     TGLLKGLADKQLGKVLLAIHNAPERDWNVEELANVALMSRSRFAAYFKQTMGVSPMDY
                     LTNWRLAVAQHLLQKGIPVALVAEQVGYSHNAALSRVFMRELGLSPTEWLAQNKES"
     gene            complement(5354..6817)
                     /gene="ubiD"
                     /locus_tag="PXD66_001167"
     CDS             complement(5354..6817)
                     /gene="ubiD"
                     /locus_tag="PXD66_001167"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HDL6319477.1"
                     /translation="MKYKNLREFLDFLESQGELVRIQQEIDPYLEMTEIADRTLRKGG
                     PALLFENPKGYQMSVLCNLFGTPKRVALGMGQEDTKALRELGKLLAFLKEPEPPKGFK
                     DLIGQLPQWKQILNMPPKVLSKADCQQVVISGDEVDLYKLPIMHCWPGDVAPLVTWGL
                     TITQGPYKKRQNLGIYRQQLIGKNKLIMRWLSHRGGALDFQEWKAENPGKPFLVSVAI
                     GADPATILAAVTPVPDTLSEYAFAGLLRGHKTEVVKSISNELEVPASSEIVLEGYIDL
                     EETALEGPYGDHTGYYNEQEYFPVFTVTHITMRKDAIYHSTYTGRPPDEPAVLGEALN
                     EVFIPILQKQFPEIVDFYLPPEGCSYRLAVVTMKKQYAGHAKRVMMGVWSFLRQFMYT
                     KFVIVCDDDVNARDWKDVIWAITTRCDPARDTTLIEHTPIDYLDFASPIAGLGSKMGI
                     DATNKWAGETTREWGTPIEKDDNVVKRIDEIWDSLGI"
     gene            6954..7928
                     /gene="znuA"
                     /locus_tag="PXD66_001168"
     CDS             6954..7928
                     /gene="znuA"
                     /locus_tag="PXD66_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HDL6319478.1"
                     /translation="MFKRTLIGLAVLGASVAQADVLTSIKPLGFIASAITEGVTETKV
                     LLPVTASPHDYSLKPSDVTQLQSAQLVVWVGDGLEGFLEKSIEKLPREKVLTLEDVDA
                     IDDIVDKASKKEDKDDDHDHKHEHKHEHKHEHKHEGHNHEGHNHDHHGHSHDEDWHIW
                     LSSEASELAAEQIAARLSEQMPEHKAKIAENLAKFKANLTAKKAEIAKQLEPVKAKGY
                     YTFHDAYGYFEREYGLNSLGSFTINPTVAPGAKTLTAIKKNIAKNKAQCLFAEPQFTP
                     KVIETLAKETKVNVGQLDPLGAKVQLSATAYPEFLQVLADEFSQCLTK"
     gene            complement(7980..8300)
                     /locus_tag="PXD66_001169"
     CDS             complement(7980..8300)
                     /locus_tag="PXD66_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005621693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnA domain-containing protein"
                     /protein_id="HDL6319479.1"
                     /translation="MIYPTCPECKSENTYHDSIQFVCPDCAYEWTGEETDQDDDVLIV
                     KDSNGNLLSDGDDVLLIKDLKLKGSSEVLKKGTKFKGIRLAQGDHNVDCGKIMLKSEF
                     LKKA"
     gene            8441..10804
                     /gene="rnr"
                     /locus_tag="PXD66_001170"
     CDS             8441..10804
                     /gene="rnr"
                     /locus_tag="PXD66_001170"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDL6319480.1"
                     /translation="MIVDPNYQQELEKYENPVPSREFILNTIREHNAPMSRDELLSAF
                     HIDDEERMEGIRRRLRAMENDGELVFTKGKRYALPEKMDLIKGTVIGHRDGYGFLQVE
                     GHEKGATKSEDWFIPNAQMVKVMHGDFVLAQPNGTDRRGRKEVRIVRVLEARKKQIVG
                     RFFLESGIGFVVPDDSRVNQDILIPDEHRMGARMGQVVVVELQERKASFSRPVGIITE
                     ILGDNLAPGMEIEIALRNHDIPHIWPEGVEKQIRQFSSEEVPEEAKQGRVDLRDLPLI
                     TIDGEDARDFDDAVFAKKEGNGWRLWVAIADVSYYVRPKTALDLEALNRGNSVYFPNR
                     VVPMLPEVLSNGLCSLNPQVDRLCLVAEMSVSSKGELQDYQFYEAVMNSHARLTYTKV
                     WKMLEGDEALRERYAPLVPHIEELYAMFQTLMKARHKRGAIEFETIENQFIFNPQGRI
                     ERIEPLIRNDAHKLIEECMILANIAAARFVEKANEPALYRIHDKPSEEKLLSFKSFVR
                     ECGLTWDVGLDPTPKDYGVLLEQIAERADKELIQTMLLRSLKQAVYAADNIGHFGLAL
                     TEYAHFTSPIRRYPDLLLHRAIKYLIEKGKGNTRYYTDGGGYHYKLDDMDEFGDKCSA
                     TERRADEATREVADWLKCEFMQDHLGEEFDGVISSVTGFGLFVKLNELLIDGLVHIST
                     LDNDYYHYDADRQRLIGGSGIIYRLGDAVRVKVINVNLDEKKIDFELLTGNKKTGKTA
                     KKKAKATEVPVFKEPKTVKKAKKVAEKKPAKSAKKRTKTTASKSRKK"
     gene            10808..11074
                     /locus_tag="PXD66_001171"
     CDS             10808..11074
                     /locus_tag="PXD66_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319481.1"
                     /translation="MDYKKQILFSLLFALVIAIISYFLFTYIPFDCSDLSKLNDSQRL
                     ECRTAEFLGVYYWLPHTTTLFFGSVLLYWAVYGMISLVQAVLKR"
BASE COUNT         3113 a         2314 c         2440 g         3313 t
ORIGIN      
        1 tagaaagttc atactttgat aaaaaaatgc ctattagtta taactaatag gcatttaaac
       61 caattaattg tacttaacca ttggttgaac gaggcgctga ctgcacttgg ctcactgcgc
      121 tggtatatcc gccaaaccct ttgccataga aatagtattt agagttttgc cattggctaa
      181 tttcgactac ttcaagcttg tcacttggtg cagaagcaat ggtcatagcg gcttgggtat
      241 gctcaacacg agagaatgta cctaatctgc cgttaaatcg ataatcgctt tcgtaagttg
      301 attttggcac ttcaatcggg ttagtaacct cttccacaat cgctggtgca accgttgctt
      361 gcgattggaa tttactgact cgctcatttt tctctttaac taaatcacgt tcagaatgct
      421 gagcaacaac actaccaaat ggagcaatat gctcttttac ctctactggt tttacgtctt
      481 cagaagcaac tttttcttct ttcggtttgg aaagctgttc ttgcttctcc ttaaccaaat
      541 cacggttagt tgcttgtgct acaaagccac ctagcggact aactgtatga agcttcttct
      601 cttctgattt tttctctgtc tcaacaagct gattttcttg ctgttcaagc atttgatcaa
      661 cagataagaa tgatgctttt tcctctactt tgacagtaga ggtaacctga acccaaactt
      721 taccgctcgc aagctcaggc gaagcaacag ctgaagttag aggcatagga gaaataacct
      781 gattgcgttg attttcacga cgacgctgat tgcccacacg caaatggcga ggtaaacggc
      841 gttggcggtt attttcacgt ccttcgttcg ttgtttctac ctcaggagct gtttcaacag
      901 gtacttcttg ggttgcaagc ggttgaattt gttgattttt ttgcacattt tcaacttgtt
      961 ttgccaccgt ttcctcaaca gcaggtgtta gattttccac ttctacttct tgtgtttcga
     1021 tacgcacttt tttacgtaga tcacggcgtt ggcgacgttc agtttgaacc tcacgggagt
     1081 tgctacgact ctcttgctcg gttacaccgg aaacttcttc cactaccgct cggcgtgctt
     1141 ttttctcgct acgcatcggt ttttcttccg ctggagtagt ttgagcttgc ggagttttac
     1201 gctcttgatt ttccgcttta gcattacggt tcgggcgttc acggcgttgg cgacgctcac
     1261 gctgattgcg tgctttcggc ttcggttttt cttccacttt ttcttcacta aaccagcctt
     1321 taatggtcgc aattaagcgg cttaacagcg atggttttgg tggttcggtt ggtgttggcg
     1381 aaggttggag attcagctcc gagctttgta acactgactc taccgtaatc accggctcgt
     1441 tacgggtaac cagcacatct tcttgatggt gatgatgtgg gtgttcttct tcatgacgag
     1501 cttcgtaatg cttggttaaa tcgtagctta acgttggagt aatttcattt tcacgcaggc
     1561 gatatacgct gaaatgcggc gtttccatac tttcagacgg tactacaaca atttgtacat
     1621 cgtggcgttt ttcaatgctg gaaatcgctt tacgtttttc gttgagtaag tagctggcga
     1681 tttgcaccgg cacaatggtg tgaacctgtg cagtgttttc tttgattgcc tcttcttcta
     1741 ataaacgtaa gattgacagt gcgattgatt cattatcacg tactttacca gttccctgac
     1801 agcgagggca gatatgcgat gaagcctcac ttaatgatgg gcttaaacgc tgacggctca
     1861 tttctaacaa accgaaacgg gaaatacggg caaattggat tctcgcacgg tcttggcgtg
     1921 tcgcctcacg aatacggttt tctacttcac gttggtggcg aaccggtgtc atatcgataa
     1981 agtcgatcac aatcaaacca cctaagtcac gcaaacgtaa ctgacgggca atttcatctg
     2041 ccgcttctaa gttggtgtta agagcggttt cttcaatatc gccgccacgg gttgaacgtg
     2101 aggagttaat atcaattgct gttaaggctt ctgttacatc aataacgata gagccaccgg
     2161 aaggtaaacg cacttcacgt tggaatgccg attcgatttg cgattcaatt tgatagtggc
     2221 tgaatagtgg tacttcgcct tcgtataatt tcacacggtt gatgaaatcc gggcgaacaa
     2281 ggcgaatgtg gtttttggct ttctcgaaca cttttttgtt gtcgattaag atttcgccaa
     2341 tatcacggcg tagatagtca cgaattgcac gcacaatcac gtcactttct tggtgaatta
     2401 agaacggagc tgggcgagat tccgccgctt ttttaatcgc ttcccagtgg tgtaacaaga
     2461 cttttaagtc ccattgtaac tcttccggcg atttgccgac accggcggta cgaacgatta
     2521 agcccacgtc ttccggtaca tctaagcagt ctaaagcctc tttgagttct aaacgctcat
     2581 caccttcaat acggcgggaa ataccaccgg cacgagggtt gttcggcatt aagactaagt
     2641 aactgcccgc aagcgaaata aaggtggtta aggctgctcc tttgttacca cgctcttctt
     2701 tgctgacttg aacaatcact tcttgccctt cttgaataat atctttgata ttcgggcgac
     2761 cgttaaacac atagtctgac gggaagtatt ctcgggaaat ttcttttaaa ggtaggaagc
     2821 cgtggcgttc tgcaccgtaa tctacgaatg cagcttcaag gcttggttcg acacgggtaa
     2881 ttttaccctt gtagatattg gattttttct gttcgtgacc cgggctttca atgtccaagt
     2941 caaataagcg ttgcccatca acgagggcaa cacgcaactc ttctttttga gttgcattga
     3001 ttaacattct tttcattgtg ttttctcatt attttgtttt tgtcttacac ggtcattttg
     3061 acccattaat aagggaatgg atttaatgtc tatctcacga ctgtcttcat tcccgatagt
     3121 gcattgatgg ttgtcttacg tttactttgt cttgtcttaa agaaactgca aaatcaatca
     3181 cttaaggctt gtttatctct attacaagcg gttatttttg ctttattttt tgcaattttc
     3241 tattgctttt tcaagccaaa aataacgagt cattatcgca ttttaaccct gaaaaaggca
     3301 aggtaattgt tgtatggtag ctgttatttg ttgtggagat gaatataatg aaaatgaatg
     3361 gggtgataag gaaaaataat gagatttagt gtgagtgctg gagataagcg tgaaatccaa
     3421 gcggttaaat ttgcaaaaaa agagctaaat ttaaccgctt gtaaagcgaa tattatgctt
     3481 ttgccgtcat ttttttcact gcttcttcag tggtccagag gtcatttgcg ataatacggt
     3541 agttggttaa tgctgcaagg tagccgtcac cttcaggaat acgaggccct gcggttgcat
     3601 ctttcaccac tgttacttca aaaccttgtt cgattaattc acgcaagtgt gaatcaatac
     3661 ataagttggc agccataccg gctaaaatta cttggtcgat tttacgtttg cgtaattgca
     3721 acactaagtc attggtttcc ggtccaaaga ttttgtgtgg agaggtaatc actgttttgc
     3781 catcaaaaat gtatggtttg tactcaggca taaagtctgc acctgaattt tcaaagcctt
     3841 ccatggtgta ttgacctttt ctcgcaaaca ttccgctgtc gtgcataaag tgttcacccg
     3901 gtgaaccaaa ttcccatttg tgatcagtcg ggtaatagta gtgtggcgaa acaaaggttg
     3961 gcatatcgac tgcttttgct gctttaaata agctttcaat gttagctaca gtgttgtttt
     4021 ctttaattga gtcacctaac acgccccaca tcacgccttt cgggcctagg aagtcaattt
     4081 gtggatcaac taccactaat gccacacgtt ttggatcaat ggtcattcca tggcgtttta
     4141 aaccgtattg ttccggctcg gcataaggtg atttttctgc actggcttgt tttgcatttg
     4201 ctgttgatgc aatacctaat gcacctaata cactggctgc cattgcaata cgagagccgt
     4261 ggttaagggc ttgacggcgt tcttggttga tttcttcaaa ttgttcctgt gacatcattt
     4321 tatcctcatt aatagatata caaattgtaa tgataacttg cgttaccgaa cgggttgcta
     4381 ttatagtgat cgcatttctc ctgttttaac cgttcgtctc aattctttac ccaaggagct
     4441 taatgtggat agtttgactc atctttttac ccaatttcag tttcgcacgg atttatttca
     4501 tctaggtcag ttatgccaaa tcgggagttt taatgaagag aataaaggct atttacattt
     4561 cgttcgtcag ggacgtttta ttttaaatca ggctgccgaa cgcccgatta cgattgaacg
     4621 cccagcgatt attttttcac caaccaatat tttgcatagt ttgcacccgt tagatgatag
     4681 tgggttagat atattttgta ttaattttga ttttggcaaa gggattcgca acccattaac
     4741 tcataccttg cacaatattg tgatcttaga gttggcacag tatccggaat tgggggtatt
     4801 agccgatcaa atttttagtg aagtgaatca gaaagggtgt ggttatcagg caatgattca
     4861 tcatttatgt gcctatttta ccttgaaagt tgcccgccaa tgtttggaaa aagggtatat
     4921 tcaaacaggt ttgttaaaag gcttggcaga taagcaactg gggaaagtgc tgttagctat
     4981 tcacaatgca ccagagcgag attggaatgt agaggagttg gcaaatgtgg cattaatgtc
     5041 tcgctcacgc tttgctgctt attttaaaca aacgatgggg gtttcaccga tggattatct
     5101 cactaactgg cgactagcgg ttgcccagca tttattacaa aaagggatac cagtagcatt
     5161 ggtggcagag caagtcggtt atagccataa tgctgctctc agccgtgtct ttatgcggga
     5221 attagggctt tcgcctaccg agtggctggc tcaaaataaa gagtcgtaac ctgatgagaa
     5281 acaaatagcc gaatggtaaa aatgtcaaaa aatttaaccg cttgttatgt tgcacaagcg
     5341 gttaaatgtt gccttaaatg ccaagagaat cccaaatctc atcaatgcgt ttcaccacat
     5401 tgtcatcttt ttcgatgggt gttccccatt ctcgggtggt ttcacccgcc catttattgg
     5461 tggcatcaat ccccattttt gagcctaacc ctgcaattgg cgaggcaaaa tcgagataat
     5521 ctatcggcgt gtgttcaatc agtgtcgtat ctcgagccgg gtcacaacgg gtggtgatcg
     5581 cccaaattac atctttccaa tctcgagcgt tcacatcgtc atcgcacaca atcacaaact
     5641 tggtgtacat aaattgacgc aggaacgacc aaactcccat catcacccgt ttagcgtgtc
     5701 cggcatattg ttttttcatc gtcactaccg ctaaacggta agaacagcct tcaggtggca
     5761 agtagaaatc gacaatttcc ggaaactgtt tttgcaaaat cggaataaag acttcattca
     5821 atgcttcacc taatactgcc ggttcatcag gtggtctgcc agtgtaggta gaatgataaa
     5881 tcgcatcttt tcgcatcgta atatgggtaa cggtaaatac cggaaaatac tcttgctcgt
     5941 tgtagtagcc ggtgtggtcg ccataagggc cttccaatgc ggtttcttcc agatcgatat
     6001 agccctccag cacaatttcg ctacttgccg gcacttctaa ctcattggaa atagatttga
     6061 ccacttcggt tttatgccct cgcaacagcc ctgcgaacgc atattccgaa agcgtatccg
     6121 gcacaggggt gactgccgct aaaatagtgg ctggatctgc accaatcgca accgacacca
     6181 gaaacggttt acccgggttt tcggctttcc actcttggaa gtctaacgca ccgccacggt
     6241 gggaaagcca acgcataatc agtttgtttt tgccgataag ttgctgacga taaatgccta
     6301 aattctggcg ttttttgtaa ggcccttgcg taatcgtcag cccccaagtg accagtggag
     6361 cgacatcgcc cggccaacag tgcataatcg gtaatttgta gagatcaacc tcatcacccg
     6421 aaatcaccac ttgctgacaa tcggctttgc tcagtacctt aggcggcata tttaaaattt
     6481 gcttccattg tggcagttgc ccgatcaaat ctttaaaccc tttcggtggt tcaggctctt
     6541 ttaaaaaggc aagtaacttg cccagctcac gcagagcttt ggtatcttct tgccccattc
     6601 ccagagctac ccgtttgggc gtgccgaata aattacacaa caccgacatt tgatagcctt
     6661 tcgggttttc aaacagtaat gccggcccgc ctttgcgtaa ggtgcggtcg gcgatttctg
     6721 tcatttctaa ataaggatcg atttcttgct gaatacggac taattcaccc tgagactcaa
     6781 ggaaatcgag aaattcacgg agatttttat acttcatagc ggtcatattt ttcgattttt
     6841 ttgcaagtat atagaaaaac gtaaatgaaa aatacccata aaaaatttat agaaattttg
     6901 ttttattttg ttagaatagc tcggttcaaa ttaaatgcca taaggggaat tttatgttta
     6961 aaagaacatt aatcggtcta gccgtgttag gtgcaagtgt tgctcaagcg gatgtattaa
     7021 ccagtattaa gcctttaggc tttattgcca gtgccattac cgaaggggta actgaaacta
     7081 aagtattatt accggtaact gcctctccac acgattacag cttaaaacct tcagatgtga
     7141 ctcaattaca atctgctcaa ttagtggttt gggtgggtga cggcttggaa ggctttttag
     7201 aaaaaagtat tgaaaaactg ccgagagaaa aagtattaac ccttgaagat gttgatgcga
     7261 ttgatgatat tgtcgataaa gcaagcaaaa aagaagataa agacgatgat cacgatcata
     7321 aacatgaaca taagcacgaa cacaagcacg agcataaaca cgaagggcat aatcacgaag
     7381 gacataacca cgatcatcac ggacatagcc acgatgaaga ttggcatatt tggctctcaa
     7441 gcgaggcaag tgagctggca gcagagcaaa ttgcggcacg tttaagcgaa caaatgcctg
     7501 aacacaaagc gaaaattgcg gaaaatcttg caaaatttaa agcaaatcta accgctaaaa
     7561 aagccgaaat tgcgaaacaa ttagagccgg taaaagccaa aggttactac acattccacg
     7621 atgcttacgg ctattttgaa agagaatatg gtttaaattc attgggttca tttaccatta
     7681 acccaactgt tgccccgggt gcaaaaacac ttacagcaat caagaaaaac attgcgaaaa
     7741 acaaagcaca atgtttattt gccgagccgc aatttacgcc gaaagtaatt gaaaccttag
     7801 caaaagaaac taaagtaaat gttggccaat tagacccact cggtgcaaaa gttcagttaa
     7861 gtgcgactgc ttaccctgaa tttttacaag ttttggctga cgaatttagc caatgtttaa
     7921 caaaataagt taaaaatccc ccatttaaat ataaatgggg gattttgcga ttacatcact
     7981 taagcttttt ttaagaattc tgatttcaac ataattttac cgcaatcgac attatggtca
     8041 ccttgagcta aacggattcc tttgaatttt gtaccttttt tcagtacttc agacgagcct
     8101 tttaatttta aatctttgat taataataca tcgtccccgt cagataataa attgccatta
     8161 ctgtctttta caattagcac atcatcatct tgatccgttt cttcgcccgt ccattcatac
     8221 gcacaatccg ggcaaacaaa ttgaattgag tcgtggtaag tattttcgct tttacattca
     8281 gggcaggttg ggtaaatcat tttaattcct caaattaata aagaaccaga ttatataccg
     8341 aaatccagca aaacgctaag cggtcatatt ttgccgattt tttaccaatc gagctaaaag
     8401 tcagttagaa tagccttaat tacacaaaaa ggaggatact atgatagtcg atccgaatta
     8461 tcagcaagaa ttagaaaaat acgaaaatcc ggtaccaagc cgagagttta ttttaaatac
     8521 aattcgtgaa cataacgccc caatgagccg tgatgaatta ttgtcggctt ttcatattga
     8581 cgatgaagaa cgaatggaag gcattcgccg ccgtctgcgt gcaatggaaa atgacggtga
     8641 attggtgttc accaaaggca aacgctatgc gttgccggaa aaaatggatt taatcaaagg
     8701 tacagttatc ggccatcgtg acggttacgg ttttttgcaa gtggaaggac acgaaaaagg
     8761 ggcgactaaa agcgaagatt ggtttattcc caacgcccaa atggtaaagg taatgcacgg
     8821 tgactttgtg ctggcacaac caaacggcac agaccgcaga ggtcggaaag aagtccgcat
     8881 tgtgcgagtg ttggaggccc gtaagaaaca gattgtcggg cgttttttcc ttgaaagcgg
     8941 tatcggtttt gttgtgcctg atgacagccg cgttaaccaa gatattctca ttccggatga
     9001 acaccgtatg ggggcgagaa tggggcaagt tgtggtggtg gaattacagg aaagaaaagc
     9061 gagttttagc cgcccggtgg gaatcattac tgaaattcta ggcgataatt tagcgccggg
     9121 aatggaaatt gaaattgccc tacgcaatca cgatattccg cacatttggc cggaaggggt
     9181 agaaaaacaa atccgccaat ttagtagcga agaagtgccg gaagaagcca agcaagggcg
     9241 ggtggatttg cgagatctgc cactgattac cattgacggc gaagatgccc gagatttcga
     9301 tgatgctgta tttgccaaaa aagagggcaa cggctggcgg ttatgggtgg cgattgccga
     9361 tgtaagttat tatgttcgcc caaaaaccgc attagatttg gaagccctaa atcgtggtaa
     9421 ctcggtctat ttcccgaatc gggttgtgcc aatgttgccg gaagtgcttt ccaatggctt
     9481 gtgttcactc aatccacagg ttgacaggct ctgcttggtg gcggaaatgt cggtttccag
     9541 caaaggggaa ttacaagatt atcaattcta cgaagcggta atgaactctc acgcccgttt
     9601 aacttacact aaagtgtgga aaatgttgga aggtgatgaa gccttgcgtg aacgctatgc
     9661 tccattagtg ccgcatattg aagaactcta tgcaatgttc caaaccttaa tgaaagctcg
     9721 ccataaacga ggggcgattg aatttgaaac cattgaaaat caatttattt tcaatccgca
     9781 agggcgaatt gagcgaattg agccgttaat ccgcaacgat gctcataaat tgattgaaga
     9841 atgtatgatt ttagccaata ttgctgccgc tcgttttgtg gaaaaagcga acgaaccggc
     9901 gttatatcgg attcacgata agccgagtga agaaaaattg ctcagtttta aatcctttgt
     9961 gcgtgagtgt ggcttaacct gggacgtagg cttagatcca accccaaaag attacggtgt
    10021 actactggag caaattgccg aacgtgccga taaagagttg attcaaacga tgttgctccg
    10081 ctcgctgaaa caagcagtgt atgcggcgga taatatcggg catttcggct tggcattaac
    10141 cgaatatgct cattttacct cgccgattcg ccgttacccg gatttactgt tacaccgtgc
    10201 aattaaatat ctgatcgaaa aaggcaaagg caacactcgc tactataccg atggcggtgg
    10261 ttatcactac aagttagatg atatggacga attcggcgat aaatgttctg ccaccgaacg
    10321 ccgagctgat gaggcaaccc gagaagtggc agattggctg aaatgcgaat ttatgcaaga
    10381 tcatcttggc gaagagtttg atggcgtgat ttcgagtgtt accggctttg gcttatttgt
    10441 aaaactcaat gagcttttaa ttgacggatt ggttcatatt tcaacgctgg ataatgatta
    10501 ttaccactat gatgcggacc gacaacgttt aatcggtggc agtgggatca tctatcgttt
    10561 aggcgatgca gttagagtaa aagtaatcaa tgtgaattta gacgagaaaa aaattgattt
    10621 tgagttactc accggtaata agaaaaccgg caaaaccgcc aagaaaaaag caaaagcgac
    10681 agaggttcct gttttcaaag agcctaaaac ggtaaaaaaa gccaaaaaag tcgctgagaa
    10741 aaaaccggca aaatctgcaa aaaaacggac aaaaacgacc gctagtaaat caaggaaaaa
    10801 atagcaaatg gattacaaaa aacaaatttt attcagcctt ttatttgctt tagtaattgc
    10861 aataataagc tattttttgt ttacctatat cccgtttgat tgtagcgatc tgagcaaact
    10921 caacgattca caacgtttag agtgccgaac tgccgaattt ctgggcgttt attactggct
    10981 gccgcacact accacgctgt ttttcggctc ggtgttatta tattgggcgg tttatggcat
    11041 gatttcatta gtgcaagcag tgctcaaacg ctaacaagca gttttgtttt tgcaaaaagt
    11101 tgcaaatctc atctgaataa tccttccaag atcaaaggtc tatcaaaaaa gatagacctt
    11161 tattgtgtaa ataaactaaa
//
LOCUS       DANLXF010000069        11024 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.069, whole genome shotgun sequence.
ACCESSION   DANLXF010000069 DANLXF010000000
VERSION     DANLXF010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11024
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.069"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            130..378
                     /gene="rpsP"
                     /locus_tag="PXD66_001172"
     CDS             130..378
                     /gene="rpsP"
                     /locus_tag="PXD66_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDL6319482.1"
                     /translation="MVTIRLTRGGAKKRPFYQIVVVDSRSPRDGRFIERIGFFNPLAA
                     GQAERLRLDVAKVDAWVAKGADLSDRVASLVKEARKAA"
     gene            411..938
                     /gene="rimM"
                     /locus_tag="PXD66_001173"
     CDS             411..938
                     /gene="rimM"
                     /locus_tag="PXD66_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDL6319483.1"
                     /translation="MSEQKIEVVGKLGSTYGIRGWLRLYSSTEQTESIFDYQPWFLKI
                     KGQWQQVELESWRYHSNDLIVKLKGTEDREAAQLLTNAEIGVDLSVFPELEEGDYYWH
                     DLIGCSVVNLEGYTMGTVAEMMETGSNDVLVVRNNGKDAFGKQERLIPFLYEQVVKRV
                     DLATKTITVEWDAGF"
     gene            983..1738
                     /gene="trmD"
                     /locus_tag="PXD66_001174"
     CDS             983..1738
                     /gene="trmD"
                     /locus_tag="PXD66_001174"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDL6319484.1"
                     /translation="MWIGIISLFPEMFKAITDFGVTGRAVKQNLLQVECWNPRDFTFD
                     KHKTVDDRPYGGGPGMLMMVQPLRDAIHAAKKAALAVEGGEAKVIYLSPQGRKLDQNG
                     VQTLASNQKLILICGRYEGVDERLIQTEVDEEWSIGDYVLTGGELPAMTLIDAVARFV
                     PGVLGKQASALEDSFAEGLLDCPHYTRPEVLDGMPVPEVLMSGHHEQIRKWRLEQSLE
                     RTWLRRPELLDSLALTDEQRVLLAKIKKQHKIS"
     gene            1765..2115
                     /gene="rplS"
                     /locus_tag="PXD66_001175"
     CDS             1765..2115
                     /gene="rplS"
                     /locus_tag="PXD66_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDL6319485.1"
                     /translation="MSNIIKQLEQEQLKQDLPSFRPGDTLEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTLRKVSNGVGVERVFQTHSPVIDSISVKRKGAVRKAKLYYLRE
                     RSGKSARIKERLGD"
     gene            2271..2627
                     /locus_tag="PXD66_001176"
     CDS             2271..2627
                     /locus_tag="PXD66_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319486.1"
                     /translation="MKQLSIRLPDDEFTFIKTFASAHKLTLQNFVMSAIQAKVAEMSA
                     KNKISEESNIHQPLSKDEKIMLNEAKVFYSARSHLKAENVILPSDFMEQIKSGCGAEK
                     MAEMMGYTKEDWESMK"
     gene            2629..2952
                     /locus_tag="PXD66_001177"
     CDS             2629..2952
                     /locus_tag="PXD66_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319487.1"
                     /translation="MGQITFSNIFTKVIFENVLSASTKEKIKQMLFQSVVALPPLHSE
                     RKMILQLKKQKITIFYQVIEEQSVQILAYQFGGTDKLTGVSKAHFKTLDAIFQMTDEE
                     KEGKF"
     gene            complement(2962..3294)
                     /locus_tag="PXD66_001178"
     CDS             complement(2962..3294)
                     /locus_tag="PXD66_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDL6319488.1"
                     /translation="MQIEQYQQWVRQFYQQQGWYERSPFMRVTYLAEEIGEVACAVRA
                     IEIGRERPDETEASQQQKRDNLIEELGDVMDNLFVLADKYGISMTEVLERHQAKFEKR
                     YLQKNMEK"
     gene            complement(3387..4583)
                     /locus_tag="PXD66_001179"
     CDS             complement(3387..4583)
                     /locus_tag="PXD66_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDL6319489.1"
                     /translation="MNIPFCLPENISPETFLREYWQKRPLLIRNGLPQIVGLFEPEDI
                     LELALEEEVTARLIQCENEKWSVKSSPLVEEDLEELPVQWSVMVQNLEQWSPELGELW
                     QAFGFIPQWQRDDIMVSCSPLNGTVGKHYDEYDVFLVQGYGQRRWQLGKWCDPSTEFK
                     PNQPIRIFDDMGELVMDEVMNPGDVLYVPSRMAHYGVSESEQGLTFSFGLRYPNATDL
                     LENLCKTIEQQADNINSSEFHLPFRLTPNEQPNALLDPKMVKMFKHQLIDLLQNSEQF
                     DELLTHSVATAASSRRYELLQTDNEYYPDEVQEILEAGGWLQQDANVKILYTENPQRV
                     YVNGEWIDELNEAEMALLIRIANGDSISWNKLASKVQDQAELELMLDTICDWLDSGWI
                     ILSEAE"
     gene            complement(4732..6075)
                     /gene="accC"
                     /locus_tag="PXD66_001180"
     CDS             complement(4732..6075)
                     /gene="accC"
                     /locus_tag="PXD66_001180"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDL6319490.1"
                     /translation="MLEKVVIANRGEIALRILRACKELGIKTVAVHSTADRELKHVKL
                     ADETICIGPAPSVKSYLNIPMIIAAAEVTNADAIHPGYGFLSENANFAEQVEKSGFIF
                     IGPTADVIRLMGDKVSAINAMKKAGVPCVPGSDGPVGNDPKKNKEIAKRIGYPVIIKA
                     SGGGGGRGMRVVHHEKDLEESIAMTKAEAKAAFNNDMVYMEKYLENPRHIEIQVLADT
                     HGNAVYLAERDCSMQRRHQKVVEEAPAQGITPELRQFIGERCANACREIGYRGAGTFE
                     FLYENGEFYFIEMNTRLQVEHPVTEMITGVDLVKEQLRVAAGLPLSITQDDIKVKGHA
                     IECRINAEDPNTFLPSPGKITRLHVPGGLGVRWDSHIYSGYSVPPHYDSMIGKLITFA
                     ENRDIAIRRMENALSETIVEGIKTNIPLHTQILADKEFRKGGTNIHYLEKKLGLK"
     gene            complement(6147..6614)
                     /locus_tag="PXD66_001181"
     CDS             complement(6147..6614)
                     /locus_tag="PXD66_001181"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDL6319491.1"
                     /translation="MDIRKIKKLIELVEESGITELEVSEEEGTVRISRAAPAVAPAAV
                     QYAAAPVVAPAAPQVASTVPVAAPAEAPKAAETSGHAVLSPMVGTFYRSPSPDAKPFV
                     EVGQTVKIGDTLCIVEAMKMMNRVESDKAGVVKEILVNDGEPVEFDQKLFIIE"
     gene            complement(6867..7649)
                     /locus_tag="PXD66_001182"
     CDS             complement(6867..7649)
                     /locus_tag="PXD66_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL6319492.1"
                     /translation="MAVKNEQFIVICNGVFRLFGNIVKILSYPFHALFPKKRFTIPEY
                     SPAKIQSSIPSKITKTIWQTNYSNKVTLPMYANYLFNRLMSLSYDYRYVSTEEREAYI
                     KANADERTFNAYSKLTDGAAQADFWRIFTLLREGGIYIDIDGHLVYPLSKIIGENDSE
                     VLIKRRDKYTNFFLASEAGNWILKDTLELIISNIENRRIEGGVFVMTGPDTLNNAIGD
                     KEVNFRRDKVTCAQGTFTNEYFQYIDKPGSKWNYKKNEDLLK"
     gene            complement(7659..8096)
                     /gene="aroQ"
                     /locus_tag="PXD66_001183"
     CDS             complement(7659..8096)
                     /gene="aroQ"
                     /locus_tag="PXD66_001183"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HDL6319493.1"
                     /translation="MKKILLLNGPNLNMLGKREPQIYGSQTLADIENHLKKLAAAQDV
                     ELESFQANGEEPIINRIHQAFQHTDFIIINPGAFTHTSVAIRDALLSVSIPFVEVHLS
                     NVHSREPFRHHSYLSDIAKGVICGLGAKGYEYALDFAISHLKA"
     gene            complement(8182..8550)
                     /locus_tag="PXD66_001184"
     CDS             complement(8182..8550)
                     /locus_tag="PXD66_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL6319494.1"
                     /translation="MAFTIRKMVEQDTHQVFPLMQKLAVFEHYIDSFAITPEVVMESG
                     FRKSPPDFYCLVAENASNIIGILVYYFLPYTAQNRPAIYMKELYVDETARNQKVGEAL
                     MQALKNEAKAHHCKTIKSLE"
     gene            complement(8610..9434)
                     /gene="dapD"
                     /locus_tag="PXD66_001185"
     CDS             complement(8610..9434)
                     /gene="dapD"
                     /locus_tag="PXD66_001185"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HDL6319495.1"
                     /translation="MSLQAIIEAAFERRAEITPKTVDAETKAAVEEVIAGLDNGSLRV
                     AEKKDGEWVVNQWVKKAVLLSFRIADNEIIDGAETKFYDKVPTKYGNYTEEQFKADGI
                     RAVPGAVVRKGSHIEKNVVLMPSFVNIGAYVGEGTMVDTWVTVGSCAQIGKNVHLSGG
                     VGIGGVLEPLQANPTIIGDNCFIGARSEIVEGVIVEDNCVISMGVFIGQSTKIYDRET
                     GEVHYGRVPAGSVVVSGSLPSKDGSHNLYCAVIVKKVDEKTLSKVGLNDLLRSIDE"
     gene            9582..10244
                     /locus_tag="PXD66_001186"
     CDS             9582..10244
                     /locus_tag="PXD66_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDL6319496.1"
                     /translation="MENFANLLTGKTREHLVPLPNALSDKHYLQPQVVEAFLTLQHAA
                     KQAGFNLQPASTFRDFERQKGIWNAKFRGERKVHDDNGVAIDMAALSDLEKCKAMLRW
                     SAVPGASRHHWGTEIDIFDPDLLPENTPLLLEPWEYLEGGYFADLTKWLHVNAETFGF
                     YFPFDGKHSRVGFEPWHISYRPISNEYEQRFNDEILKAAWQDEEVAGKECLLEHFEEL
                     LK"
     gene            10241..10993
                     /locus_tag="PXD66_001187"
     CDS             10241..10993
                     /locus_tag="PXD66_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL6319497.1"
                     /translation="MIQLINETQQHDKFRQICDKWQLVEDKSSPLALVLTEERLELRK
                     LDEPKLGAIAVNFVDGTMAHRRKFGGGRGEAVAKAVGIKGDYLPTVIDATAGLGRDAF
                     VLAAVGCKVLLVERNPIVAALLEDGLNRAYQDVEIGSFMQERMILADVRDISQLDPQS
                     QMAEVVYLDPMYPHKQKSALVKKEMRVFQHLVGADLDSDSFFLPAKGLAKKRVVVKRP
                     DYAPFLAEQKPDFSHTTKNHRFDVYLSHLKND"
BASE COUNT         3150 a         2236 c         2384 g         3254 t
ORIGIN      
        1 tgaatctccc ttgtctttcc gcaaaaaatc tgtataattt gaccgcttgt tttctctttt
       61 gaaacaagtt cgttaccctc acctcgaacg agggtttatt ttttactatc aacatttaga
      121 ggaaaggtta tggtaaccat tcgtttaact cgtggcggag ctaaaaaacg cccattttac
      181 caaatcgtgg tagtggacag ccgttcacct cgtgatggtc gctttatcga gcgtatcggt
      241 ttcttcaacc cattagcagc aggtcaagct gagcgtttac gcttagatgt ggctaaagta
      301 gatgcttggg ttgctaaagg tgctgattta tcagaccgtg ttgcttcttt agtaaaagaa
      361 gctcgcaaag cagcttaatt tttaagccaa aattaaataa gtaggtgagt atgagcgaac
      421 aaaagatcga agttgtcgga aaattaggtt caacctatgg tatccgtggt tggttgcgtc
      481 tctattcatc gacagaacaa actgagagca ttttcgacta ccagccgtgg tttttaaaaa
      541 ttaaaggtca gtggcagcag gtcgaattag aaagctggcg ttatcatagc aatgatttga
      601 ttgttaaatt gaaaggcact gaagaccgtg aagccgcaca gttattaacc aacgctgaaa
      661 ttggcgtgga tttgtctgtg tttcctgaat tggaagaggg agattactat tggcacgatt
      721 tgatcggctg ttcggtagta aacttagagg gctacacaat gggaacagtt gccgaaatga
      781 tggaaacagg ttcaaatgat gtgcttgtgg tgcgtaataa cggcaaagat gctttcggta
      841 aacaagaacg attaattccg tttttgtatg aacaagtagt taaaagagta gatctcgcca
      901 ccaaaaccat tacggtggag tgggacgctg gtttctaacc tcaggtatgc ggtgagcttt
      961 ggaaaacatt taaggcgtta aaatgtggat tggtattatt tcactgtttc ctgaaatgtt
     1021 taaagcaatt accgatttcg gggtgacagg acgtgcagtt aaacaaaatc tactgcaagt
     1081 tgaatgttgg aatcctcgtg attttacatt cgataaacat aaaactgtag atgatcgccc
     1141 ttatggcggt ggtccgggaa tgttgatgat ggtgcaacct ttgcgtgatg cgattcacgc
     1201 agcgaaaaag gcggcattag cagtagaagg cggtgaagct aaagtgattt atctttcgcc
     1261 gcaaggacgt aaactcgatc aaaacggtgt gcaaacactg gcttcaaatc agaaactgat
     1321 tttaatttgt ggacgatatg aaggcgtgga cgagcgattg attcaaacag aagttgatga
     1381 agaatggtca atcggtgatt acgttctcac agggggggaa ttacctgcaa tgacattaat
     1441 tgatgcagtt gcaaggtttg ttcccggggt attaggcaaa caggcttccg cgttagagga
     1501 ttctttcgca gagggattat tagattgccc acattatacc cgccctgaag tattagacgg
     1561 tatgcctgtt ccggaagtgc tgatgtctgg acaccatgaa caaattcgta aatggcggtt
     1621 ggaacaatcg ctggaacgaa cgtggttaag acgccctgag ctattggata gcctagctct
     1681 gactgacgaa cagcgtgtgt tgttagctaa aattaaaaaa cagcacaaaa tcagttaatt
     1741 ctaggattat agaaggtttt taaaatgagt aacatcatca aacaacttga acaagaacag
     1801 ttaaaacaag acttacctag cttccgtccg ggtgacacat tagaagttaa ggtatgggta
     1861 gtagaaggta gtaaaaaacg tttgcaagcg ttcgaaggcg tggttattgc aattcgtaac
     1921 cgtggcttgc actctgcatt cactctacgt aaagtttcaa atggcgtagg cgttgagcgt
     1981 gtattccaaa ctcactcacc tgtaattgac agcatttctg ttaaacgtaa aggtgcggta
     2041 cgtaaagcta aactttacta cttacgtgag cgttctggta aatctgcacg tatcaaagag
     2101 cgtcttggcg actaattgct acaaaacagt gaaatgcaca aaagccttga ggaaactcaa
     2161 ggcttttgct ttttgtactt acaagcagtt atttttcctc aatgatttac aaatcttgct
     2221 ttcttttttg ttataatgtt aacaagataa cattaatgat aggtgtcgat atgaaacaat
     2281 taagtattag attgccagac gatgaattta cttttattaa aacatttgct tctgctcata
     2341 aactgacttt acaaaatttt gtgatgtccg caatccaagc taaagttgca gaaatgtctg
     2401 ctaaaaataa aatatctgaa gaaagtaaca ttcatcaacc tctttctaaa gatgaaaaaa
     2461 taatgctaaa tgaggcaaaa gtattttatt ctgctcgttc gcatttaaag gcagaaaatg
     2521 tgattcttcc gagtgatttt atggagcaaa ttaaatcagg ctgtggagca gaaaaaatgg
     2581 cagaaatgat gggatatacc aaagaagatt gggagtcaat gaagtaatat ggggcagatt
     2641 acatttagta atattttcac aaaagtgatt tttgagaatg tgctttctgc ttcaactaaa
     2701 gagaaaataa aacaaatgct ttttcagtct gtggttgcat tacctcctct ccattcagaa
     2761 agaaaaatga ttttacagtt gaaaaagcaa aaaattacca ttttttatca agttattgag
     2821 gaacaatctg tgcaaatcct cgcttatcaa tttggtggaa ctgataaatt gacaggtgtg
     2881 tctaaagctc actttaaaac actagatgcc atttttcaaa tgacggatga agagaaagaa
     2941 ggaaaattct aatgtaagcg gttatttttc catatttttt tgcaaataac gtttctcaaa
     3001 ttttgcttga tggcgttcaa gcacttccgt catcgaaatg ccatatttat cggctaatac
     3061 aaaaagatta tccatcacat cgcctaactc ttcgattaag ttatctcgtt tttgctgttg
     3121 tgaagcttcg gtttcgtccg gtcgttcacg cccgatttca atcgctctta ctgcacaagc
     3181 cacttcgccg atttcttctg ctagataagt cacccgcata aaagggcttc gttcatacca
     3241 accttgttgt tggtaaaact ggcgaaccca ttgttgatat tgttcaattt gcattttatt
     3301 tctgattcaa aaagtaggga cgaaaaattt ttcgcccttt tataagaaaa tgtatcaggg
     3361 caaatgatga tttgccccta ggagtgttac tctgcctcag agagaataat ccagccacta
     3421 tccagccagt cgcaaatggt gtctagcatt agctccagct ccgcttggtc ttgcactttg
     3481 gaggcaagtt tgttccacga aatcgaatct ccattggcga tacgaatcag taatgccatt
     3541 tctgcttcgt ttaactcatc aatccactcg ccatttacat aaacccgttg agggttctcg
     3601 gtgtagagga tttttacatt tgcatcttgc tgtaaccagc cgccggcttc gagaatttct
     3661 tgcacttcgt cagggtaata ttcattgtca gtttgcaata attcataacg gcgggagctt
     3721 gccgcagtgg caacactgtg agtgagtaac tcatcgaact gttcggaatt ttggagcaag
     3781 tcgattaact gatgtttaaa cattttcacc attttcggat caagcaacgc attcggctgt
     3841 tcattcggcg ttaagcggaa cggaagatga aattccgatg aattaatgtt gtcggcttgt
     3901 tgttcgatgg ttttgcagag attttccagt aaatcggtcg catttggata acgtaaacca
     3961 aaagagaaag ttaaaccttg ctcgctttcg gaaacaccat agtgagccat tcgagacggc
     4021 acataaagca catcacccgg gttcattacc tcgtccatta ccagttcgcc catatcatca
     4081 aaaatgcgga tcggttggtt tggtttgaat tcggtgcttg gatcgcacca tttgcctaat
     4141 tgccaacgac gttgcccata gccttgcacc aagaacacat cgtactcatc ataatgtttg
     4201 ccgacagtgc cgttgagcgg tgagcaagaa accataatgt catcacgttg ccattgcggg
     4261 ataaaaccaa aagcctgcca aagctcgcct agctcaggag accattgctc gaggttttgc
     4321 accattacag accattgtac aggtaactct tccaaatctt cctcaacaag cgggctggat
     4381 ttaacagacc atttctcgtt ttcgcattga atcaggcgtg cagtgacttc ttcctctaat
     4441 gccagttcta aaatatcttc cggctcaaac aatccgacaa tttggggtaa gccgttacga
     4501 attaatagcg gtcgtttttg ccaatattct cgcaaaaagg tttcagggct gatattttca
     4561 ggtaagcaga atggaatgtt cataatggct ccttgctacg tgcttctttg agattactga
     4621 tattctaccc gagaaatcag gaaaaacggg aaatttttta tgatttagcg acatcattca
     4681 cattttgaag aggaaataat gaaaaaggcg aacattgttc gcctttgaaa tttattttaa
     4741 accgagtttt ttctcgagat agtggatatt ggtgccacct ttgcggaatt ctttatccgc
     4801 aagaatttga gtatgtaaag gaatattggt tttaatgcct tccacaatgg tttcggaaag
     4861 ggcattttcc atccggcgaa tcgcaatatc acgattttca gcaaaggtaa tcaatttacc
     4921 aatcattgaa tcgtaatgtg gcggcacgct atagcctgag taaatatgtg aatcccaacg
     4981 cacacctaaa ccacccggaa cgtgtaaacg ggtaatttta cccggcgatg gcaagaaggt
     5041 gtttggatct tctgcgttga tacggcactc aatggcgtga cctttcactt taatatcatc
     5101 ttgtgtgatc gaaagtggta agccggctgc cacacgcagt tgctctttta ccaagtccac
     5161 accggtaatc atttcggtta cagggtgttc tacctgtaag cgggtgttca tttcaatgaa
     5221 gtaaaactcg ccgttttcat ataagaactc aaaagtacct gcaccacggt agccaatttc
     5281 acggcaggca ttggcacaac gctccccaat aaattgacgc aattcaggtg taataccctg
     5341 agcaggtgct tcttccacca ctttttggtg gcgacgttgc attgaacagt cacgctctgc
     5401 taaataaacc gcattaccgt gagtatcggc aagtacttgg atttcaatat ggcgtggatt
     5461 ttctaaatat ttctccatat acaccatatc gttattaaat gccgctttcg cttcggcttt
     5521 cgtcattgcg atagattctt ccaaatcttt ctcgtgatga accacacgca taccacgacc
     5581 gccaccacca cctgatgctt tgatgatcac cgggtagccg atacgtttag cgatttcttt
     5641 atttttcttc ggatcgttac caaccggacc gtcagaaccc ggtacgcaag gcacgccggc
     5701 ttttttcatt gcattgatag cagagacttt atcgcccatt aagcgaatca catcagccgt
     5761 tggaccaata aagataaagc ctgatttctc aacttgttcg gcaaagtttg cgttttcaga
     5821 taagaaaccg tagcccggat gaatagcatc ggcattcgtt acttcggcag cggcaataat
     5881 catcgggata tttaaatagc tttttaccga tggagcagga ccgatacaaa tggtttcatc
     5941 ggctaattta acgtgtttta attcacggtc ggcagttgaa tgcactgcaa ccgttttaat
     6001 gcccaattct ttacaagcac gcaaaatacg cagggcaatt tcaccacggt ttgcgataac
     6061 aactttttct aacataggga aattccttgt gttctagaaa atgtagggcg tgttgcacac
     6121 gcccgttgga taatttaagg aagagcttat tcaatgatga ataatttttg gtcgaattct
     6181 accggttcgc catcattaac taagatttct tttaccacac cggctttgtc tgactcgaca
     6241 cggttcatca ttttcattgc ttctacaata cataacgtat caccgatttt cacggtttga
     6301 cccacttcaa cgaacggttt tgcatctggg cttgggctac ggtagaaagt ccccaccatt
     6361 ggtgaaagca ctgcgtggcc actcgtttct gcggcttttg gtgcttcagc cggagctgca
     6421 acaggtactg tcgaagctac ttgaggtgcc gctggtgcaa caacaggagc tgctgcatat
     6481 tgaactgcag ctggtgcaac agctggagca gcacggctaa tacgcaccgt accttcttct
     6541 tcagacactt ctaactcggt aatgccagat tcttcaacta attcaataag ttttttgatt
     6601 ttgcgaatat ccataataag attccgtatg tgattaaata aaataaaaaa gagaactcca
     6661 catttggggt ataaattcaa atgtagagtt aaaaaatgct gggcgtagaa tacctgaatt
     6721 tcgctaattt tgcgaataaa atctgataat tttggcgaaa atggacgatt ttagcattca
     6781 tcgtaccagt taggattttt ggaaaatggt gactcacaag cgattgaaaa ttgccaattt
     6841 tttgtaaaaa acaaccgctt gtcagattat ttcaataaat cttcattttt cttgtaattc
     6901 cacttgctac cgggcttgtc gatatactga aaatattcgt tggtaaaagt accttgagca
     6961 caagttactt tgtctcggcg gaaatttact tctttatcac caatggcatt atttagggtg
     7021 tcaggaccgg tcataacaaa cacgccacct tcaatccgac gattctcaat attgctgata
     7081 atcagctcaa gggtgtcttt tagaatccaa tttccagctt ccgaagccaa gaaaaagttg
     7141 gtgtatttat cacggcgttt aattaacact tcgctatcat tttcgccaat aattttagag
     7201 agtgggtaaa ccaaatgccc gtcaatatca atataaatgc caccttctcg gagcaaagta
     7261 aaaattcgcc aaaaatcagc ttgtgccgca ccatcggtta atttgctata ggcgttaaag
     7321 gtgcgttcat cggcgttggc cttgatgtat gcctctcgtt cttctgtgct aacgtagcga
     7381 taatcgtagc tcaatgacat taggcggtta aacagataat tcgcatacat tggcaaggtg
     7441 actttgttag aataattcgt ttgccaaatg gttttggtaa ttttgctcgg aatgcttgat
     7501 tggatttttg ccgggctata ttccggaatg gtgaaacgtt ttttcggaaa aagtgcatgg
     7561 aacggataac tcaatatttt gacgatatta ccgaataaac ggaagactcc gttacagatc
     7621 acaataaatt gttcattttt aacagccatt ttattacctt atgcttttaa gtgtgaaatt
     7681 gcaaaatcca acgcatattc atagcctttt gcccctaaac cgcaaataac gccttttgca
     7741 atatcactta gataggagtg atggcgaaaa ggttcacggc tatgaacgtt agacaaatgc
     7801 acctcaacaa acggaatcga cacactcaat aacgcatcac gaattgccac gctggtgtga
     7861 gtaaatgccc cggggttaat aataataaaa tcggtatgct gaaaggcttg gtgaatacgg
     7921 ttgataatcg gctcttcacc gttggcttga aagctctcca gctcaacatc ttgggccgca
     7981 gccaattttt ttaagtggtt ttcaatatcc gccaaagttt gcgaaccgta aatttgtggc
     8041 tcacgtttgc ccaacatatt taaattcggg ccgttaagga gaaggatttt tttcatattt
     8101 acttattcaa cttgtaattc ataattaagc cattcacggt tttctttcgc ccccaagcgt
     8161 tgataaaaac gaataccacc gtcattccaa ggattttatt gtcttacaat gatgtgcctt
     8221 cgcctcattt ttcaaggctt gcattaacgc ctctcccact ttctgattcc gagcagtttc
     8281 atcaacataa agctctttca tataaatagc ggggcggttt tgggcagtgt aaggaagaaa
     8341 ataataaacc aatatgccga taatgttaga cgcattttca gccactaaac agtaaaaatc
     8401 gggtgggctt ttgcgaaagc cggattccat tacaacttca ggagtaatcg caaaagaatc
     8461 gatatagtgc tcaaacacag ccagtttttg catcagaggg aacacttggt gggtatcttg
     8521 ttcgaccatt ttgcggattg taaatgccat aaaattcctt acaagcggta aaaaaatccc
     8581 tcacttttgc aaagcgaggg aggttttaat tattcgtcaa tagaacgtaa taagtcgttt
     8641 aagcccactt tgcttaaggt tttctcatcc acttttttca caatcaccgc acagtagagg
     8701 ttgtgtgaac catcttttga tggaagcgag cctgaaacca caacagagcc tgccggcaca
     8761 cggccgtaat gtacttcgcc ggtttcacgg tcgtaaattt tggtagattg accgataaac
     8821 acgcccattg agatcacgca gttatcttcg acaatcacgc cttctacgat ttcagaacgt
     8881 gcaccgataa agcagttgtc gccgatgatg gttgggttcg cttgtaatgg ctctaacaca
     8941 ccaccgatgc ccacgccacc agaaaggtga acatttttac cgatttgcgc acaagaaccc
     9001 actgttaccc aagtgtccac cattgtgcct tcgccaacgt atgcaccgat gttcacgaaa
     9061 gatggcatta acaccacgtt tttctcgata tgtgaacctt tacgcaccac cgcacccggc
     9121 acggcacgaa tgccatctgc tttgaattgc tcttcggtgt agttgccgta tttggttggc
     9181 actttgtcgt agaatttggt ttctgcaccg tcgatgattt cattgtcggc aatacggaat
     9241 gaaagtaata ccgctttttt cacccattgg tttactaccc actcgccatc ttttttctcg
     9301 gcaacacgca gtgaaccgtt gtctaatcct gcgatcacct cttctactgc tgctttggtt
     9361 tcagcatcaa cggttttcgg agtaatttcc gcacggcgtt caaatgccgc ttcaataata
     9421 gcttgtaaag acattgttgt gttccttcaa tatataaatt aaaacagaaa attcggctca
     9481 ctttttaaca tagaaaggcg tttatttcca caagaaaaaa catatcgcag aaatttagtt
     9541 tataattcca agccacttat ttgatacaag cggtgatttt tgtggaaaat tttgcaaatc
     9601 tgcttaccgg taaaacgcgt gagcatttag tgccgttgcc gaacgctctt tcggataaac
     9661 attaccttca gcctcaggtg gttgaggctt ttttgacgtt gcagcatgct gcaaaacaag
     9721 ccggctttaa tttacagcct gccagtactt ttcgtgattt tgaacgccaa aaagggattt
     9781 ggaatgcgaa gtttaggggt gagcgtaagg ttcacgatga taacggggtt gcgattgata
     9841 tggcggcgtt atcggatctt gaaaagtgca aagcaatgtt gagatggtct gcggtgccgg
     9901 gggcaagtcg gcatcattgg ggaacggaaa ttgatatttt tgaccctgat ttattgccgg
     9961 aaaatacgcc tttattgctt gagccgtggg aatatttgga aggtggctat tttgccgatt
    10021 taaccaagtg gttacacgtt aatgcggaaa catttggctt ttatttcccg tttgacggta
    10081 aacattctcg agtgggcttt gagccgtggc acattagcta tcgcccgatt tcaaatgaat
    10141 atgagcaacg ctttaacgat gaaatcttaa aagctgcgtg gcaagatgaa gaagtggcgg
    10201 gtaaggaatg cttacttgaa cattttgaag aattactaaa atgatccaat taattaacga
    10261 aacccaacaa cacgacaaat tccgccaaat ttgcgacaaa tggcagctgg ttgaagataa
    10321 atccagcccg ttagccttag tgctaacgga ggaacgattg gaactacgca aattagacga
    10381 acccaagctc ggggcgattg cagtgaattt tgtggacggt acgatggcac atcggcgtaa
    10441 attcggtggt gggcgaggtg aagccgttgc gaaagcggtc ggcattaaag gggattattt
    10501 gccaactgtg attgatgcga cagccggttt agggcgagat gcttttgtat tagcagcggt
    10561 tggttgtaaa gtgctgttag tggaacgcaa tccaattgtc gctgccttgt tggaagatgg
    10621 tttaaaccga gcttatcaag atgtggaaat cggcagtttt atgcaagaaa gaatgatctt
    10681 agccgatgtt cgagacattt ctcagctaga tccacaatcc caaatggctg aggtagtgta
    10741 tttagatccg atgtatccgc acaaacaaaa aagtgcgttg gtgaaaaaag aaatgcgggt
    10801 gtttcaacat ttagttgggg cagatttaga ttcggacagc tttttcttac cggcaaaagg
    10861 attggcaaaa aaacgtgtgg tagtgaagcg tccggactat gccccgtttt tagcggagca
    10921 aaagcctgat ttttcacaca ccaccaaaaa tcaccgtttt gatgtttatc tttctcacct
    10981 gaaaaatgat taaaggtggg tcatgcgata ggcaagcggt tatt
//
LOCUS       DANLXF010000070        10931 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.070, whole genome shotgun sequence.
ACCESSION   DANLXF010000070 DANLXF010000000
VERSION     DANLXF010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10931
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.070"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            80..979
                     /gene="corC"
                     /locus_tag="PXD66_001188"
     CDS             80..979
                     /gene="corC"
                     /locus_tag="PXD66_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249295.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HDL6319498.1"
                     /translation="MSDEQQSVNTTEKSEKKSILHSLFSGLFQNEPKNREDLVEVIRD
                     SVENELIDSDTKEMIEGVMEISELRVRDIMIPRAQIVFIDANEPLDVCIDLIIESAHS
                     RFPVIRGEKDTIEGILHSKDLLKYLRSDSEPFNLQQVLRPAVIVPESKRLDRMLKEFR
                     SERFHMAVVVDEFGAVSGLVTIEDILEQIVGDIEDEFDEEEVKPIRQLSRHTYAVLPL
                     TDIKKFNEFFATDFNDEEVDTIGGLVMQAFGYFPKRDEQIELNGIDFKVTSANSRQLI
                     QLRVTLSDEQLEKMEKTSPNEEE"
     gene            1038..2546
                     /gene="lnt"
                     /locus_tag="PXD66_001189"
     CDS             1038..2546
                     /gene="lnt"
                     /locus_tag="PXD66_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HDL6319499.1"
                     /translation="MNFAKNPPFLTACLLAIILGAIGTFAYSPFDIWILAFASAAGLV
                     WSATLANRKTALWATFAWSIGYFCTGVNWVSVSMTQFGGVPTIVSYIAVFLLACYLAI
                     YNLLFSVVSHRLKLNNPFALAAIFTFTEYLRGVVFTGFPWLQFGYSLIDSPFAGIAPI
                     LGVEGLTFLVIVASSYLVKIIQQENKTVPSLMVLAVIFGLSFATRFLQFVQIDTEKQP
                     LVVSLVQGNIEQKMKWDPQHFNHSVRTYEQLILPLLGNSELIILPESAIPALENQISP
                     LLGSLDHLARQHGSEIIIGTLYQNEQEELFNSSVLLGEKEREYALHSNPRYNKHHLVP
                     FGEYVPFGSVLDWMREVFILPINLSQGSFVQPSFLAKNNRFNMAICYEIIFGDQLQQN
                     QKSQNSDYLLTITNDAWFGSSIGPWQHFQMARMRALELGKPLLRAANTGITAIVDANG
                     KVIEQLPQFTADALTATIVPTKGDTPFKQLGHWLIYSFSLFCVIASLLLRRK"
     gene            complement(2592..3056)
                     /locus_tag="PXD66_001190"
     CDS             complement(2592..3056)
                     /locus_tag="PXD66_001190"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDL6319500.1"
                     /translation="MATFQGNYIATGLKFGIVATNWHKIFIDQLLNGATDKLLRHGVE
                     EKNIDTVWVPGALEISIAAKKMAQSGRYDAIICLGAVVRGATSHYDVVVNESAKGISS
                     SALETGVPIINGILTVENLEQAIERSGTKAGNKGEECAMVAIEMANLLKALA"
     gene            complement(3262..4461)
                     /locus_tag="PXD66_001191"
     CDS             complement(3262..4461)
                     /locus_tag="PXD66_001191"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HDL6319501.1"
                     /translation="MFKFSTVEEAIQAIAQGKIILVSDDADRENEGDFICAAEFATPE
                     NINFMAKYGKGLICTPISTEIAKKLNFHPMVLENEDNHQTAFTVSVDHIQTGTGISAF
                     ERSLTCLKMLDENAKPEDFRRPGHVFPLVAKDGGVLVRNGHTEATVDLARLAGLKPAG
                     LCCEIMAEDGTMMQMPELQAFAQAHNMPFITIQQLQEYRRKHDSLVNVVSVVKMPTKY
                     GEFKAHSFVETISGKEHVALVKGDIGDGENVLCRIHSECLTGDAFGSQRCDCGQQFAA
                     AMNQVEQEGRGVVLYLRQEGRGIGLINKLRAYELQDRGMDTVEANLALGFKDDEREYY
                     IAAQMFEKLGVKSIRLLTNNPAKIEGLIEQGLNVVAREPIMVEPNEHDLEYLKVKQHK
                     MRHMFNF"
     gene            complement(4480..5127)
                     /gene="ribE"
                     /locus_tag="PXD66_001192"
     CDS             complement(4480..5127)
                     /gene="ribE"
                     /locus_tag="PXD66_001192"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDL6319502.1"
                     /translation="MFTGIIEEVGQIAQIKKQGEFVVVTVKAKKILTDVHLGDSIAVN
                     GVCLTVTSFTQEQFTADVMSETLKRTSLGELSINSSVNLERAMAANGRFGGHIVSGHI
                     DGTGVVAEITPADNSTWYRIKAEPKLMRYIIEKGSITIDGISLTVVDVDAESFRVSII
                     PHTIKETDLGTKKIGSLVNLENDIVGKYIEQFLLKKEPENPQSKITADFLKNAGF"
     gene            complement(5344..6441)
                     /gene="ribD"
                     /locus_tag="PXD66_001193"
     CDS             complement(5344..6441)
                     /gene="ribD"
                     /locus_tag="PXD66_001193"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005708727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDL6319503.1"
                     /translation="MTDVQYMAYAITLAEKARGWTSPNPLVGCVIVKNGNIIAEGYHQ
                     KAGSWHAERNAILNCEEDLTGATAYVTLEPCCHHGRTPPCSELLIERGIKKVFIGSRD
                     PNPLVSGKGAAQLRAAGIEVVEDFMRTECDALNPIFFHYIQTKRPYVLLKYGMTADGK
                     IATYTGNSKWITGEAARANVQKSRHEYSAIMVGVETVLADDPMLNSRMENAKQPVRIV
                     CDSQLRTPLDSQLVQTARQYRTIIATLNNDAKKQAEYTACGVEILVTGERDKRVDLAD
                     LLQKLGELGIDSLLIEGGSQLNFSVLEQGLVNRVHCYIAPKLIGGKAAKTPIGGKGIG
                     ELAQAVKLRLKSTELLGEDILLDYEVDLLRN"
     regulatory      complement(6617..6735)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            6939..9341
                     /gene="lon"
                     /locus_tag="PXD66_001194"
     CDS             6939..9341
                     /gene="lon"
                     /locus_tag="PXD66_001194"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HDL6319504.1"
                     /translation="MAPRKKKAIELPLLPLRDVVVFPYMVMPLFVGREKSIQALRSAM
                     DSNKQLFLVTQQDPNKEEPNAEDMYGVGVIANIIQMLNLPDGTVKVLVEGQTRAKIEQ
                     IHDDENGFWAAIQPIYSEYDDENEELKAIAKTTLTEFENYVKNNKKIPAEIIAKLQKI
                     TLEDRLADTIASNLIAPVKKKQELLEQPNLIARFEALLIAMATEMDTLETETRIRNRV
                     KQQMEKNQRDYYLNEQIKAIQKELGNGDDVEQSELDKLKEKIDAAKLPTEVKDKLNTE
                     FKKLKAMPQSSSEATVVRGYIDWILQMPWHKKSAVKKDLAQAQEILDKDHYGLERVKE
                     RIVEYLAVQSRLNKLKGPILCLVGPPGVGKTSLGQSIANATGRKYVRMALGGVRDEAE
                     IRGHRRTYIGSMPGSLMMKMAKVGVRNPLFLLDEIDKMAQDMRGDPASALLEVLDPEQ
                     NKAFNDHYLEVDYDLSDVMFVATSNSMNIPPALLDRMEVIRLSGYTEDEKMHIARGHL
                     LAKQQENNGLKEGELTIEDSAILSIIRYYTREAGVRSLEREIAKICRKAVKALVLDKK
                     LKSITVSEKNIEEYLGVKRFDYGKMDSQNRIGEVTGLAWTEVGGDLLTIETASVAGKG
                     KFSYTGSLGDVMKESIQAAMVVVRSRAEKLGIAEDFHEKRDIHVHVPDGATPKDGPSA
                     GIAMCTALISSLTGNPVRKEVAMTGEISLRGKVLPIGGLKEKLLAAHRGGITTVIIPK
                     ENEKDLEEIPENAKAALDIHAVETIDEVLALALENPPMGIEILSKAEIKVKKSRAKAT
                     VQ"
     gene            complement(9475..10365)
                     /gene="ilvY"
                     /locus_tag="PXD66_001195"
     CDS             complement(9475..10365)
                     /gene="ilvY"
                     /locus_tag="PXD66_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HDL6319505.1"
                     /translation="MDFQSLKLFLDIAQSRSFIRSAEKNYMTASTLTRHIQRIEEELG
                     QPLFLRDNRQVRLTEAGERFLAFAQQGWAEWQQLQNQLSPAQGDLEGELKVFCSVTAS
                     YSHLPPILGKFRQKYPKVDIKLSTGDPALALEQVQSLAADISVAGKPEFLPNNIVFHY
                     IDDIQLSIIAPRVASQATQFLQQSPIDWKNIPFILPVNGPVRKRIDRWFKEQKIKDPI
                     IYATVSGHEAIVPMVALGFGVALLPDIVIKHSPMNNQVSYVNLPSPITPFELGICVQQ
                     KRLQEPIINAFWQLLSDNSA"
     gene            complement(10367..>10931)
                     /locus_tag="PXD66_001196"
     CDS             complement(10367..>10931)
                     /locus_tag="PXD66_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDL6319506.1"
                     /translation="HFSEIYQDALALIGFENIETQYDVPLNATSNQSIKHYLYENQIR
                     NYIALNLFGAGSARRFSEQKINELLSCLKQHSDKPIVLLTFPEVTAKLCSFTKKYKNI
                     FVYEKTKSVFDSIELIRFADLLISPDTATVHIASGLSKKIIAFYSSDEQNFIHWHPNN
                     QAETHILRFRKSVNDLDFAAIKPEWLK"
BASE COUNT         3146 a         2376 c         2248 g         3161 t
ORIGIN      
        1 agtgaaaata gcaatgtttt atttgctttg tgactgaatt tgcttattat aagcgtactt
       61 tatttaagtg gaacaaacta tgagtgatga acagcaaagt gtaaacacca ccgaaaaatc
      121 agaaaaaaaa tcgattttac actctctatt tagcgggctt tttcaaaacg agcctaaaaa
      181 ccgtgaagat ctagtcgaag tgattcgaga ctctgttgaa aatgaattga tcgacagcga
      241 caccaaagag atgatcgaag gagtaatgga aatctcggaa ttacgagtgc gggatattat
      301 gattccccgt gcgcaaattg tgtttattga tgccaatgaa cctttagatg tctgtattga
      361 tttaattatt gaatccgcac actctcgttt tccggtgatt cggggtgaga aagatactat
      421 cgaaggtatt cttcactcta aagatttatt gaaatattta cgttctgact ctgaaccctt
      481 taatctgcaa caggtattac gccctgcggt tattgtaccg gaaagcaaac gcctagaccg
      541 aatgctaaaa gagttccgtt cagaacgctt ccatatggca gtagtggttg atgaatttgg
      601 tgcggtttca ggcttagtca ccattgaaga cattcttgaa caaattgtgg gtgatattga
      661 agatgagttt gatgaagaag aggtcaaacc aattcgccag ctttcaagac atacttatgc
      721 ggtattaccg ctcaccgaca tcaaaaaatt caatgaattt tttgctaccg actttaatga
      781 cgaagaggtg gataccattg gtggtttagt gatgcaggct ttcggttatt tcccgaaacg
      841 agatgagcaa attgagttaa acggaattga ttttaaagtc acctcagcta atagccgcca
      901 attgattcag ctacgagtca cactttccga tgaacagtta gagaaaatgg aaaaaacctc
      961 tcctaacgaa gaagaataat atagtgagtt tcaatcaagg cacgtttcgg cgtgcctcat
     1021 ttatttagtg agttattatg aattttgcaa aaaatccgcc ttttttaacc gcttgtctcc
     1081 ttgcaatcat actgggtgca atcggcactt tcgcctattc gccttttgat atttggattc
     1141 ttgcctttgc ttccgcagcc ggcttggttt ggtcagccac tctggcaaac agaaaaaccg
     1201 cattatgggc aacctttgct tggtctatcg gctacttctg caccggtgta aactgggttt
     1261 ctgtgagtat gacccaattt ggtggtgttc ctaccatagt aagctacata gcagtattcc
     1321 tgttggcgtg ctatctcgct atttataatc tgctgtttag tgtagtttca cacaggctca
     1381 aactgaataa tccgtttgca ttggctgcga tttttacctt taccgaatat ttacgaggcg
     1441 tggtatttac cggttttccg tggctacaat tcggctatag cttaatagac agcccctttg
     1501 ctggtattgc gccgattttg ggcgttgaag gtttgacttt cttggtaata gttgcaagta
     1561 gctatctagt gaaaattatt cagcaagaga acaaaactgt gccatcattg atggtactag
     1621 cggtcatttt tggcttaagt tttgccactc gctttctcca atttgtgcaa attgatacag
     1681 aaaaacaacc gcttgtagtg agtttagtac aagggaatat tgagcaaaaa atgaagtggg
     1741 atccgcaaca tttcaaccac agcgtacgaa cctacgaaca gctcattctg cccctattag
     1801 gtaacagtga actgattatt ttgcctgaat ccgccattcc ggcacttgaa aatcaaatca
     1861 gcccgctgct tggctcgctc gatcacttag ctcgccaaca cggaagtgaa attattatcg
     1921 gcacactcta tcaaaatgag caagaggaac tgtttaacag tagcgtgcta ttaggcgaaa
     1981 aagagaggga atatgccctg cacagcaacc ctcgctacaa caaacatcac cttgtgccgt
     2041 ttggcgaata tgtgccgttt ggctcggtgc tggattggat gcgggaagtg tttattctac
     2101 caattaatct ttcacaaggc agtttcgttc aaccatcatt cttggcgaaa aacaaccgat
     2161 ttaatatggc gatttgctat gaaattattt ttggtgatca gttacaacaa aaccaaaaaa
     2221 gccaaaattc agattattta ttaactatca ccaatgatgc gtggtttggt tcttccatcg
     2281 ggccttggca acatttccaa atggcaagaa tgcgagcgtt agaacttggc aaaccattgc
     2341 ttcgagctgc gaataccggt attaccgcca ttgttgatgc caacggaaaa gtaattgaac
     2401 aactaccgca atttactgct gatgcactaa ctgccaccat tgtgccaacc aaaggcgata
     2461 cgccgtttaa acaattaggt cactggttaa tttacagttt cagcctattt tgtgtcattg
     2521 ccagcctgtt actacgcaga aaataaaaaa atccccttag gctcttgggt ctaaggggat
     2581 tttatcgctg attacgctaa ggcttttaat aggttcgcca tttcaatcgc taccattgca
     2641 cactcttcgc ctttattacc ggctttagtg cctgaacgct caatcgcttg ctctaaattt
     2701 tccaccgtta aaataccgtt aataatcggc acgccggttt ctaaagcaga agagctgatg
     2761 ccttttgctg actcgttcac taccacatca tagtggctgg ttgctccacg cacgaccgca
     2821 cctaagcaga taatcgcatc ataacgaccg ctttgtgcca tttttttcgc tgcaattgag
     2881 atttctaatg cacccggcac ccatacggtg tcgatatttt tttcttctac gccgtgacgc
     2941 aataatttat cggttgcacc gtttaacagt tgatcgataa aaatcttgtg ccagttagtt
     3001 gccacaatac cgaattttaa accagttgcg atgtagtttc cttgaaatgt tgccattgtt
     3061 tgttttcctt ctttaaaata aatttgatac tcaataaccc tctccccttg tgggagaggg
     3121 atagattttt cttcgttcag aagaaaaatc agggagaggg gaaattgcaa actttttcta
     3181 aaaaatatac cctctctctg aaaaatactt ctgaacgaag tatttttctg tctctctccc
     3241 acaaggggag agagtattgg attagaaatt aaacatatgt cgcattttgt gctgtttaac
     3301 tttaaggtat tccaaatcgt gttcgtttgg ctcaaccata atcggctcac gagccactac
     3361 gtttaaacct tgctcaatga gtccttcaat ttttgccgga ttattggtta acaaacgaat
     3421 cgattttacg cctaattttt caaacatttg agcggcgata taatattcac gctcatcatc
     3481 tttgaaacct aatgccaggt tggcttcaac cgtgtccatt cctctatctt gcaattcgta
     3541 agcacgcagt ttattaatta aaccgatgcc acgtccttct tggcgtaaat aaagcaccac
     3601 gcctctgccc tcttgctcca cttggttcat tgccgctgcg aactgctgac cacaatcgca
     3661 acgttgtgag ccaaaggcat cgccggttaa acattcggaa tggatacggc ataatacatt
     3721 ttcgccatca ccaatatcac ctttaactaa agcgacgtgt tctttgcctg aaatagtttc
     3781 aacgaaactg tgtgctttga actcaccgta tttggtcggc atttttacca ccgaaaccac
     3841 attcaccaag ctgtcgtgtt ttctacgata ctcttgtaac tgctgaatag taataaacgg
     3901 catattgtgt gcttgtgcaa atgcctgtaa ttccggcatc tgcatcatcg tgccgtcttc
     3961 tgccataatt tcacagcaaa gccctgccgg tttcaagccc gccaaacgag ctaaatccac
     4021 agttgcttcg gtatgaccgt tacgcactaa tacgcctccg tcttttgcca ctaatgggaa
     4081 aacatgcccc ggacgtctaa aatcttccgg cttagcattt tcatcaagca ttttcaggca
     4141 agttaatgaa cgctcaaaag cggaaatacc ggtgccggtt tgaatatggt caacagaaac
     4201 ggtaaatgcg gtttgatgat tatcttcgtt ctctagcacc atcgggtgaa aatttaattt
     4261 tttggcaatt tctgtcgaaa tcggcgtaca aatcaaccct ttaccatatt tcgccataaa
     4321 gttgatattt tccggtgtcg caaattccgc tgcacaaatg aagtcccctt cattttcacg
     4381 gtcggcatca tcgctgacta aaataatttt cccttgtgca attgcttgaa ttgcttcttc
     4441 taccgttgaa aacttaaaca ttatttgtcc tttatttggt taaaaccctg catttttaag
     4501 aaagtccgca gtaattttac tttgcggatt ttcaggctct tttttcaata aaaactgttc
     4561 gatatatttg ccgacaatgt cattttctaa attcaccaaa ctaccgattt ttttcgtacc
     4621 gaggtcggtt tccttaatgg tatgcggaat aatcgatacc cggaaagatt ccgcatcaac
     4681 atccaccacc gttaaactaa tgccatcaat agtaattgag cctttttcaa tgatgtaacg
     4741 cattaactta ggttcggctt taatgcgata ccaagttgaa ttatctgctg gtgtaatctc
     4801 tgcaaccaca cccgtgccat caatatgacc tgacacaata tgcccaccaa aacgcccgtt
     4861 tgccgccatt gcccgctcaa gattaaccga gctgttgata cttaattcgc caagtgatgt
     4921 acgttttaag gtttctgaca taacatcagc agtaaattgc tcctgcgtaa atgaagtcac
     4981 ggttaaacac acgccattta ccgcaatgct atcccctaaa tgtacatcgg tcagaatctt
     5041 ttttgcttta accgttacca ccacaaactc gccttgtttc ttaatttggg ctatctggcc
     5101 aacttcttcg ataatgcctg tgaacattta aatttttctc cgatttactt ccaatttagt
     5161 ctgagaactg cttatcttac tattcccctc tcccgtttac gggagaggga agagaaaatc
     5221 cgtaggattt tcggagggag agggaagatt acactcattt tcccctctct ctgcataaaa
     5281 tttctgaacg aaattttatg ctgtctctct cccgtaaacg ggcgagagta aggaaatttc
     5341 aatttaatta cgaagcaagt ccacttcata atccaacaaa atatcttctc cgagcaattc
     5401 ggtggatttt agacgtaatt taaccgcttg tgctaactcg ccaatcccct tgccaccaat
     5461 cggggttttt gccgctttgc cgccaatcag tttcggggca atatagcaat gtactcgatt
     5521 gactaacccc tgttccagca cgctgaaatt gagttgcgaa ccaccttcaa tcaataagct
     5581 gtcgatcccg agttcgccca gcttttgcaa aagatcggct aaatccaccc gcttgtcacg
     5641 ttcgcctgtc actaaaattt caacaccgca agcggtatat tctgcctgct tttttgcatc
     5701 attattgagc gtagcaataa tggtgcgata ttggcgtgca gtttggacta gttggctatc
     5761 caatggggta cgcagttggc tgtcacatac aatccgcacc ggctgtttag cattttccat
     5821 tcggctgttg agcattgggt catcggcaag caccgtttcc accccgacca taatcgcact
     5881 atattcgtgg cggcttttct gtacattagc tcgggcagcc tcgccggtga tccatttcga
     5941 attccccgta taagtcgcaa ttttaccatc tgccgtcatc ccatatttca gcaacacata
     6001 agggcgtttg gtttgaatgt aatggaagaa aatcgggttc agcgcatcgc actccgttcg
     6061 cataaagtct tccacaacct caatgccggc ggctctaagc tgtgccgctc ctttgccaga
     6121 aacaagtggg ttcggatctc gagagccgat aaacactttt ttgatcccac gttcaatcag
     6181 cagttcggaa caaggcgggg ttcgcccgtg gtggcaacaa ggctctaagg ttacataagc
     6241 ggttgccccc gtcagatctt cctcgcagtt taaaatcgca ttgcgttcag cgtgccaact
     6301 accggctttt tggtgatacc cttcggcgat aatgttgccg tttttcacga tcacacagcc
     6361 aactagcgga ttagggcttg tccaaccccg tgctttttcg gcaagggtga tggcataagc
     6421 catatattga acatctgtca tatacatttt gataaattga gggcgtaatg aaaggattat
     6481 tgcaatacaa gcggtcgttt ttagtaaaaa atttgcaaac gccgcctgca aaacaatgaa
     6541 gccttgaaat ctcacgactc aaggcttgca aaaaagacac aaaaaatgac cgcttgtgcg
     6601 gtattttttg cggtttttga ctcttctttc atccagacta tactgtcggt tttggaattt
     6661 caccaaatcc tgccgattgc tcggctcgcg gactttaccg ccgatcggga atttcaccct
     6721 gccctgaaga ttaaaatatg caggggattt tgctcgcatt acgatttgat gtcaagcggg
     6781 aaattaagct aattaccctg attttaacaa ttgattaaat cgccaacatc tactgttttg
     6841 actattcgag tttttttaag ataaatcaaa ttttcccttg tttttcccaa attcgccctc
     6901 ataatggggg attatatttt gatagagaga gcaaatttat ggcaccaaga aaaaagaaag
     6961 cgattgaatt accactactc ccattgcgtg atgtggtggt ttttccttat atggttatgc
     7021 cgttatttgt aggacgtgaa aaatcaattc aagccctacg ctcggcgatg gattccaata
     7081 aacaactgtt tttagtcact cagcaagatc caaataaaga agagccgaat gcagaagata
     7141 tgtatggcgt aggtgtaatt gccaacatta ttcaaatgtt aaacctgccg gacggcacag
     7201 ttaaagtgct ggtagaagga caaacccgtg ccaaaatcga gcaaattcac gatgatgaaa
     7261 acggcttctg ggcggcaatt cagccaatat attcggaata tgatgacgaa aacgaagagt
     7321 taaaagcgat tgctaaaacc actttaaccg aatttgaaaa ttacgttaaa aataacaaga
     7381 aaattccggc ggaaattatt gctaaactgc aaaaaatcac gttggaagac cgcttggcgg
     7441 ataccattgc ctctaactta attgctccgg tgaagaaaaa acaggaatta ttggagcaac
     7501 caaatctgat tgcccgtttt gaagccttgt taattgcgat ggcaactgag atggatacgc
     7561 ttgaaaccga aactcggatt cgtaatcgtg ttaaacagca aatggaaaaa aaccaacgtg
     7621 attactacct caacgagcaa attaaagcga ttcaaaaaga gttaggtaat ggcgatgacg
     7681 ttgaacaaag cgagttagat aagcttaaag agaaaattga cgctgcaaaa ttaccgaccg
     7741 aggtaaaaga caagctgaat acggagttca aaaagctcaa agcaatgccg caaagctctt
     7801 ccgaagctac cgttgtgcgt ggttatattg actggatttt acaaatgccg tggcataaaa
     7861 aatcagcggt caaaaaagat ttagcccaag ctcaagagat tttggataaa gaccactacg
     7921 gcttagaacg agtgaaagag cggattgtag aatacttagc agtgcaaagt cgtctaaata
     7981 aattaaaagg tccgattttg tgcttagttg gtccgccggg ggttggtaaa acctcattag
     8041 ggcaatctat tgccaatgcc acagggcgta aatatgtgcg tatggcgtta ggtggcgtgc
     8101 gtgatgaagc ggaaatccgt gggcatcgcc gtacttacat cggctcaatg ccgggtagcc
     8161 taatgatgaa aatggcgaaa gtcggcgtgc gtaatccgct gttcttgtta gatgaaatcg
     8221 acaaaatggc tcaagatatg cgtggcgacc cggcttctgc tctgcttgaa gtgttagatc
     8281 ctgagcaaaa taaagccttt aacgaccatt atttagaggt ggattacgat ttatccgatg
     8341 taatgtttgt ggcaacctca aactcaatga acattccgcc agcattgctc gaccgtatgg
     8401 aagtgattcg tctttcaggc tataccgaag atgagaaaat gcacattgct cgtggtcatt
     8461 tactggctaa acagcaagaa aataacggac taaaagaggg tgagctgacc attgaagaca
     8521 gtgcaatttt aagtattatc cgctactaca cccgtgaagc aggcgtgcgt agccttgagc
     8581 gtgaaattgc gaaaatctgc cgtaaagccg tgaaagcttt agtattggat aagaaattaa
     8641 aatccattac agtttctgag aaaaatatcg aagaatactt aggcgtaaaa cgttttgact
     8701 acggcaaaat ggatagccaa aaccgcattg gcgaagtgac cggtttagcg tggacagaag
     8761 ttggcggtga tttattaacc attgaaaccg cttctgttgc aggtaaaggc aaattctctt
     8821 acaccggttc attaggcgat gtaatgaaag agtctattca agcggcaatg gtggttgtac
     8881 gctctcgtgc agaaaaatta ggcattgctg aagacttcca cgaaaaacgg gatattcacg
     8941 ttcacgtgcc ggacggtgca acaccgaaag atggtccgag tgcagggatt gcaatgtgta
     9001 ctgcattaat ttccagccta acaggtaacc ctgtgcgtaa agaagtagcg atgaccggtg
     9061 aaatcagctt gcgtggcaaa gtattgccaa tcggcggctt aaaagagaaa ttactggcag
     9121 cccatcgtgg tggtattacg acggtgatta ttccgaaaga aaatgaaaaa gacttagagg
     9181 aaattccgga aaatgcaaaa gcagcgttgg acattcacgc agtagaaacc attgacgagg
     9241 tactagctct ggcactagaa aacccgccaa tggggattga aatcttgtca aaagccgaga
     9301 tcaaggtgaa aaaatcgcga gctaaagcca cagtgcaata aagtgcaata atctaaaaca
     9361 acataaggtg cgcagatcac aatcacgcac cttatttttt atctgaaagt tttatctaca
     9421 agcggtcggt tttgctctat tttttgcaaa aatttagcaa aatctaaccg cttgttatgc
     9481 tgaattatca gacaataatt gccaaaatgc gttgatgatc ggctcttgca agcgtttttg
     9541 ctgaacgcaa attcccagct caaacggagt gatcggcgag ggtaaattca cataagaaac
     9601 ttgattattc attgggctat gcttaatcac aatatccggt agcagtgcga ccccaaagcc
     9661 tagtgcaacc atcggcacaa tcgcttcgtg tccggagacg gtagcataaa taatcgggtc
     9721 tttgattttt tgctctttaa accaacggtc aatccgtttt cgtacaggtc cgttcaccgg
     9781 caaaataaag gggatatttt tccaatctat cggcgactgt tgcagaaatt gagtggcttg
     9841 gctcgccaca cgaggggcaa taatcgaaag ttgaatatca tcaatatagt gaaacacaat
     9901 attattcggc agaaattccg gcttacccgc aaccgaaata tctgcagcca atgactgaac
     9961 ctgttccaat gccaaagccg gatcaccggt actcagttta atatccactt tcggatattt
    10021 ttggcgaaac tttcccaaaa tcggcggtaa atggctgtaa gaagccgtta ccgagcagaa
    10081 cactttcaac tctccttcta aatcaccttg agcaggcgac aactggtttt gcaactgctg
    10141 ccactctgcc caaccttgtt gagcaaacgc caaaaacctt tcccctgctt cagttaaacg
    10201 cacttgtcga ttatcacgca gaaacaacgg ctgcccgagc tcctcctcta tacgctgaat
    10261 atggcgggta agtgtggaag ccgtcatata attcttctcc gcactgcgaa taaaactacg
    10321 gctttgggct atatctagga aaagtttaag gctttggaag tccatattat tttagccact
    10381 ccggttttat tgcagcaaaa tctaaatcat tcacgctttt tctaaaacgg agaatatggg
    10441 tttcagcctg attattcgga tgccaatgta taaaattctg ttcatcgcta ctgtaaaaag
    10501 caattatctt tttactcaag cctgaggcaa tatgcacggt tgcagtatca ggtgaaatta
    10561 ataagtccgc aaaacgaatc agttctattg aatcaaaaac gcttttggtt ttctcataga
    10621 caaatatatt tttatacttc ttagtaaagg aacacaattt tgccgttact tcaggaaaag
    10681 taagtaagac tatcggttta tcactatgct gttttaagca actcagcagt tcattaattt
    10741 tctgctcact aaaacgtctt gccgaacctg caccaaaaag atttaacgca atataatttc
    10801 taatttggtt ttcatataaa taatgcttta tggattggtt gcttgtggca tttagtggca
    10861 catcatactg agtttctata ttttcaaacc cgattaaagc taacgcatct tgataaatct
    10921 ctgaaaaatg c
//
LOCUS       DANLXF010000071        10840 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.071, whole genome shotgun sequence.
ACCESSION   DANLXF010000071 DANLXF010000000
VERSION     DANLXF010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10840
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.071"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(157..987)
                     /gene="fghA"
                     /locus_tag="PXD66_001197"
     CDS             complement(157..987)
                     /gene="fghA"
                     /locus_tag="PXD66_001197"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HDL6319507.1"
                     /translation="MQQFENYRCFSGEQQVWQHFSASLNCEMKFAIYLPQSAKTQKLP
                     VLYWLSGLTCTEQNFITKSGFQRYADEHHLIVVAPDTSPRGVNVANDEAYDLGQGAGF
                     YLNATQGEWTTHYQMYDYIVSELPTLINAHFPTNGKQSIFGHSMGGHGALTIAFKNPG
                     QYQSVSAFAPIVAPTQVPWGQKAFLAYLGEDQTAWKAYDSVALLEKYRPSLPILIDQG
                     SQDSFLSDQLKPEIFCETADRLGIPYQFNLRDGYDHSYYFISSFIGEHIAFHAKHLKD
                     "
     gene            complement(1067..2125)
                     /locus_tag="PXD66_001198"
     CDS             complement(1067..2125)
                     /locus_tag="PXD66_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HDL6319508.1"
                     /translation="MAYPIKSYATFSATTPLAPHSFERRELLEDDVLIDILYCGVCHS
                     DLHMARNDWGWTQSYPLVPGHEIIGRIIKVGKNAKKFQIGDLVGVGCMVDSCRECSPC
                     QHGLEQYCEVGNIGTYGGIDRFDGTPTQGGYSTCVVVSEDFVLKVSENLDTKAVAPLL
                     CAGITTYSPLRHWNVKAGSKVAVVGLGGLGHMAVKLAKAMGAEVTLFTRSLGKADDAY
                     RLGASRVVLSTDEKQMADVANTFDLIIDTVPYTHDLKPYIPTLALDGTLVLVGLVGEL
                     EQTINTVPMILGRRSISASVIGGIQETQEMLDFCAEHNIVPDVEMIDMQNINDAYERM
                     LKSDVKYRFVIDMGSLKG"
     gene            complement(2312..3247)
                     /locus_tag="PXD66_001199"
     CDS             complement(2312..3247)
                     /locus_tag="PXD66_001199"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03571 family LLM class oxidoreductase"
                     /protein_id="HDL6319509.1"
                     /translation="MNTLSPIFSQVFGQNQLTLGLIAPFMGYQNHIPDNLTTIADNAR
                     LAENLGFSALWVRDVPFYDPHFGDVGQGLDPMVTLGFLSAHTQNIALGTAGLIAPLRS
                     PIHIAKMATSVDSLSHGRFLLGLSSGDRPVEYPAFGADFASRSERFRESIDLINTLTE
                     QDFPHFKGKHYADLTDNLDLLPKPKHRLPIVAIGRARQEMDWLANVPDAWIWHGVNPA
                     DTANIINTLADLRQKDTPTAFGYAQFVEVLDDKNAKAQLFNNIYLRGGAKGLADFWAK
                     EQEKGVKHLALNLKPTQRPADEVLTELAEYALTQF"
     gene            complement(3527..3838)
                     /locus_tag="PXD66_001200"
     CDS             complement(3527..3838)
                     /locus_tag="PXD66_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL6319510.1"
                     /translation="MSETEKQAINAPNAVMNGYLTMHYPDWFKPDGIYFNDGAFESFE
                     SSHKLTKDGKIRLVPTAGHTLGHLAVVVDMGEHYILIGGDASYSEQDMLAGNIDGVCN
                     A"
     gene            complement(3845..4237)
                     /locus_tag="PXD66_001201"
     CDS             complement(3845..4237)
                     /locus_tag="PXD66_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL6319511.1"
                     /translation="MIKVHCLTTGWVQIKIHHQVARFFARPARVLDVLTDKKWSPKLP
                     IGCWLIEHDDGLILVDTGESSRANDKGYQPWWHPFMQFCERRGVKKHEEVGEILKANG
                     FDPLRIDTVVMTHMHGDHAGGLPNFPNS"
     gene            4369..4929
                     /locus_tag="PXD66_001202"
     CDS             4369..4929
                     /locus_tag="PXD66_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDL6319512.1"
                     /translation="MNDLRVIKTHLAIQNALIELLDEKLFEKVQVQEIIEKALINRTT
                     FYRYYSGESDLVGKLIKEVKAEYQDLLKKRFESGNLLRFMQTIGNRLLEKRRLILALW
                     QIKTPRHHLYDDMHMLLKNAFIAHAKRITSDDKNWAEKNWDYQAHIFATIALESHKYY
                     FEQGKLLPIPQVFEEWIEMVQVLKSV"
     gene            complement(4995..5984)
                     /locus_tag="PXD66_001203"
     CDS             complement(4995..5984)
                     /locus_tag="PXD66_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012073273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL6319513.1"
                     /translation="MSKQIQFTKTGSPDVLQIVDVAIPAPKAHEVQIQIQAIGLNRAE
                     MMYREGAYVIDPVFPATLGYEGAGVIVAIGEGVNEVAISDKVSIIPSFMFTEYGTYGE
                     IVNMPKHAVVKHPDNLSMEQASASWMAFVTAYGGLIEFGKVQKGDFVVLGGATSSVGL
                     ASIQIAKMQGATVIALSRTHAKGDVLLEKGADFVIATSEDDVTAKLFEITNGKGVNVV
                     FDPVGRKEAAKIINAMAQDGRYIIYGALSHDDIAVPVFPILGKHLTVRGYELFEITTV
                     PEKLAQAKTFVYDGLASGQLRPEIDKIFAFDEMVKAHEYMASNQQIGKIVVKV"
     gene            complement(6198..7115)
                     /locus_tag="PXD66_001204"
     CDS             complement(6198..7115)
                     /locus_tag="PXD66_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL6319514.1"
                     /translation="MTFIIGILAGVLIAFQSAINARLRMHVGAAFLTSFVSFSVGLLF
                     LLGLSLIMQQTPAFSLDNLGNVPLWAWTGGLLGMVGLTANVLIFPKLGGVQTAVMPIL
                     GQVLMGVLIDTFAWLNSPYIAFTASRLFGIGLVLFGVWIAVVLPHLSGLKRKENANLW
                     LWRAVGILGGTALATQAAVNGEFGRQPGSSLGAATVSFGVGAIGLLLVVLFYEKSLPK
                     LCQPTEGKPFWIWSGGILGSLFILAGVWLVPQIGTGSVVMLVLSGLICGSLLVDKFGL
                     FGITQKPILPLQIIGVTLLLAGVAFIRLL"
     gene            complement(7251..7910)
                     /locus_tag="PXD66_001205"
     CDS             complement(7251..7910)
                     /locus_tag="PXD66_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003672168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL6319515.1"
                     /translation="MQFFNKEDVINAFHYRASTRSYDGSRKIPAEDFNYILELGRLSP
                     SSVGSEPWQFLVLQNPELRQKIKPYCWGIPTMETSSHIVVILAKKNARYDSQYFSEIM
                     DRRGLQGEAREKAMAVYKKFQEKDIGILDSERSLFDWASKQTYIALGNMMTGAALIGV
                     DSCPIEGFAYQEVNDILAAEGLFDPSEWGVSVMCTFGYRDKEIRQKARKDFETVVKFI
                     E"
     gene            complement(8095..9159)
                     /locus_tag="PXD66_001206"
     CDS             complement(8095..9159)
                     /locus_tag="PXD66_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HDL6319516.1"
                     /translation="MAKFRKLFTPFNIKNLALKNRVVMPPMCQYSATNGVPNDWHFVH
                     YTSRAVGGVGLIIVEMTNVAPNGRITPNCLGLWNDEQQQAFKKLVDSVHAQGAKIGVQ
                     IAHAGRKAQDEPDAVAPSAIHYGELDYAGQNLITPRELTTDEVKALVQAFKDSVKRAV
                     EAGFDTIEIHGAHGYLIHQFYSPKSNQRSDEYGQDKMLFGEQVIQAAREVMPSEMPLI
                     VRISAQEYGENGFGLDYGLQVAKRFADAGADVLDISGGGDGVLHAGNHPEFYAGYQVD
                     FARQVKLATGKPVIAVGMLDNPAVGDHILGMGDADLVAVGRALLRDPYWVLNAQYDQN
                     AADSSEMQFVPKQYERGLFM"
     gene            9250..10158
                     /locus_tag="PXD66_001207"
     CDS             9250..10158
                     /locus_tag="PXD66_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL6319517.1"
                     /translation="MSDIRTLDFNLLKAFVVLLDECNVSRAAQRLSITQPAMSGILNR
                     LRESFNDPLFVRVQHGMQPTDRALQLGQTARNILQDISSMLQPPILEPEHLNMTLRIA
                     AMDYVQQIIALPLILRLRRLAPNVKVALLPVQGQNIKTLFEQKKIDLALVSRQHLSTD
                     TLKTVLYEERYVCAMSKTHPMAQTPLSLDQFCNLPFAMLSYNGGEFSGATDLALQKIG
                     LQRKVMVSVNHISLLPQLLQGSDLVAVLPERLAKTLPNVYLQPPPIEVEGFTMMMAWH
                     ERTEQDTAHRWLREVLQGVVKPKQAL"
     gene            complement(10228..10779)
                     /locus_tag="PXD66_001208"
     CDS             complement(10228..10779)
                     /locus_tag="PXD66_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL6319518.1"
                     /translation="MAKQIAVLIGSGSKTSFSHLTVRHLQKMAPASIQLNIVDIADLP
                     LYDRDLDENSPAQYTRVREAVAVADGVLLVTPEHNASFSAMLKNAIDVVSRPMGQSKW
                     IGKPAGIVTVGASPAGGVRAADQVRTITSGSFVNMPTLPFALNVGNLFTGVFDEQGEI
                     ASETLKSSMQNFINAYAQFVEKY"
BASE COUNT         2996 a         2477 c         2341 g         3026 t
ORIGIN      
        1 ttattaaagc acgacttttg ttgtatttca aacctttgtt tttatacaaa acaaccgtgt
       61 tgtaaaaaat gatcgtaaac tgaccgcttg taatgcactc tgaatatatc gaaacatgat
      121 aaaaatgata aagcatgttt cgataattta ctgtctttag tcttttaaat gttttgcatg
      181 aaaggctata tgttcaccga taaagctact aatgaaatag tagctgtgat cgtaaccatc
      241 acgtaaattg aattggtagg gtattccaag tctatctgcg gtttcacaga aaatttccgg
      301 ctttaactga tcgctcaaaa agctgtcttg gctgccttga tcgattagaa ttggcaagct
      361 tgggcgatat ttttccagca atgccacgct atcatacgcc ttccaagcgg tctgatcttc
      421 gcctaaatat gctaaaaatg ctttttgccc ccaaggaact tgggttgggg cgacaatcgg
      481 tgcaaatgcc gatacacttt gatattgccc cggattttta aatgcaatgg ttaaggcacc
      541 atgtccgccc attgaatgcc caaaaattga ttgtttaccg ttagtcggga aatgagcatt
      601 aatcaatgtt ggcagttcgc tgacaatgta atcatacatt tggtaatgtg ttgtccattc
      661 gccttgcgta gcatttaggt aaaatccggc accctgacct aaatcataag cctcatcatt
      721 agcgacattc acacccctag ggctggtatc cggtgcgacc acaatcagat gatgttcgtc
      781 ggcataacgt tgaaagccag atttagtgat gaagttctgc tcggtacagg ttaaaccaga
      841 aagccaatat agtacgggta gcttttgtgt tttagcagat tgcggcagat aaatagcaaa
      901 tttcatttca cagtttaagc tggctgagaa atgttgccaa acctgttgtt caccgctaaa
      961 acagcggtag ttttcaaatt gttgcataat attccttaca agcggtttaa ttccgctgat
     1021 tttttgcaaa aataaaggcg gaactcaccg cctttatatt gtaatatcaa cctttcaaac
     1081 ttcccatatc aatcacaaac cgatatttca cgtctgattt cagcattcgt tcataagcat
     1141 cattgatgtt ttgcatatca atcatttcca catccggcac aatgttgtgt tcggcgcaga
     1201 agtcgagcat ttcttgagtt tcctgaatgc cgccgatgac agaggcggaa atagagcggc
     1261 ggcctaaaat catcggtacg gtgttgatgg tttgctccaa ctcaccgacc aagcccacca
     1321 gcaccaatgt gccgtccaac gctaaggttg ggatataagg tttcaagtcg tgggtataag
     1381 gcacggtatc gatgattaaa tcaaaggtat ttgcaacgtc tgccatttgc ttttcatcgg
     1441 tagaaagcac cacacggctt gcgcccaaac ggtaagcatc atcggcttta cccagtgagc
     1501 gagtaaaaag cgtaacttcc gctcccattg ctttggcaag tttaaccgcc atatgcccca
     1561 gtccgccaag ccccacaaca gcgacttttg agccagcttt gacattccaa tgacgcaggg
     1621 gcgaataggt ggtaatgcct gcacacaaaa gaggggcaac cgctttggtg tccaagtttt
     1681 cagagacttt taatacaaaa tcttcactca ccaccacgca tgtgctgtaa ccgccttgtg
     1741 ttggtgtgcc gtcaaagcgg tcaataccgc cataagtgcc gatgttgccc acttcgcagt
     1801 actgctccaa accgtgctgg caaggactgc attcacggca ggaatccacc atacaaccga
     1861 caccgaccaa atcgccgatt tggaattttt tggcgttttt accgactttg ataatacgac
     1921 cgatgatttc gtgccctggt acaagcggat agctttgcgt ccagccccaa tcattgcgag
     1981 ccatatgcaa gtctgaatgg cacacgccac agtacaaaat atcaatcaaa acatcgtctt
     2041 caagcaattc acggcgttca aaactgtgtg gggcaagtgg tgtggttgcc gaaaaagtgg
     2101 cgtaggattt gatagggtaa gccatttttg gggtttaaat ggctgaaatg gcaattacta
     2161 tagtgccgat gatttattat tgaaaatcaa gatttttgat aaatcggtat cagttaaaat
     2221 gataattggg ggttttcaga ctgactgaaa aattattttg cccattttaa aaacaaaaac
     2281 accgccctca tcacaaaagc ggtgtttttc atcaaaattg tgtcaaagca tactctgcca
     2341 attctgtaag cacttcatca gcaggacgct gagtgggttt taaattgagt gccaaatgtt
     2401 tcacgccttt ttcttgttct tttgcccaaa agtctgccaa gccttttgca ccaccacgca
     2461 gataaatgtt gttaaataac tgtgctttgg cgtttttgtc gtccaaaact tccacaaatt
     2521 gagcatagcc aaatgctgtg ggggtgtctt tttggcgtaa atcggcaagg gtgttgatga
     2581 tgtttgcggt gtcggcagga ttcacaccgt gccaaatcca cgcatcaggt acattagcaa
     2641 gccagtccat ttcttgcctt gctcgtccaa tcgccacaat gggcaagcgg tgtttgggtt
     2701 tggggagcaa atccaaattg tctgttaaat cggcataatg tttgcctttg aaatggggaa
     2761 aatcctgctc ggttaaggtg ttaatcaaat caatgctttc acgaaaacgc tcgctacgag
     2821 aggcaaaatc cgccccaaat gcagggtatt ccacaggtct atcgccagag gataagccca
     2881 ataaaaaccg tccatgactc aaactatcca cacttgtcgc cattttggca atgtgaatgg
     2941 gactacgcaa aggggcaatc agccctgccg tgccaagggc gatgttttgg gtgtgggcgg
     3001 acaaaaaacc aagtgtaacc atcgggtcaa gcccttgccc cacatcgcca aaatgagggt
     3061 cataaaacgg cacatctcgt acccacaatg ctgaaaagcc caagttttca gccagccgtg
     3121 cattgtcggc aatggtggtc aaattgtctg gaatgtgatt ttgatagccc ataaaggggg
     3181 caattaagcc cagtgttaat tgattttgcc caaaaacttg ggagaaaatg ggggaaagcg
     3241 tgttcatttt ggcgtacctg tcttatttaa gataaggtat tttaagggct tttgagtttg
     3301 cttaaaatgg gataaattga gaaagtgttt tgctaaattg gcataaatag acgctttcaa
     3361 gctgactgaa aatttgcaaa atttttaata aaaatgaccg cttgcctttc aacaagcggt
     3421 cattcccctt taatttttta ccaccgtaaa caccttattt ttcagccgat attcactttt
     3481 ttcatcgtga gccagttgca caatggttgg cttgcgttta cacaattcac gcattgcaca
     3541 cgccgtcaat gttgccagca agcatatctt gttcagagta agaggcatcg ccgccaatca
     3601 aaatataatg ctcgcccata tccaccacca ccgccaaatg ccccagcgta tgtcctgcgg
     3661 ttggcacaag gcggattttg ccgtctttgg tgagtttatg actgctctca aaactttcaa
     3721 acgccccatc attaaaatag atgccatcag gcttaaacca atcaggatag tgcattgtta
     3781 aataaccgtt catcaccgca ttgggggcgt tgattgcctg tttttcagtc tcactcatca
     3841 caaatcagct gtttgggaaa ttgggcaatc cgcctgcgtg gtcgccgtgc atatgggtca
     3901 ttaccaccgt gtcaatgcgt aaagggtcaa agccgtttgc ctttaaaatt tcgcccactt
     3961 cttcgtgttt tttgacccca cgccgttcgc aaaactgcat aaacgggtgc caccacggct
     4021 gatagccctt gtcgttggca cgactgcttt cgcccgtatc caccaaaatc aaaccgtcat
     4081 cgtgttcaat cagccagcaa ccaatcggca gtttaggcga ccatttttta tcggtcagca
     4141 catcaagcac acgggcagga cgggcaaaaa agcgtgccac ttgatgatga attttgattt
     4201 gtacccaacc tgttgtcaga caatgcactt taatcatttt tgtgttctca ttaaaaaatc
     4261 gtgggcatat tgtagaaagt gcggtaactt ttcacaaaca ttaaaaagtt cgtatttggc
     4321 tggatatgca gataaaatta caaaaatgta tgcttatttt gaaaaaagat gaacgattta
     4381 agagtaatca aaacccatct agcaatccaa aacgctttga ttgaattatt agatgaaaag
     4441 ctgtttgaaa aagtgcaagt ccaagaaatc attgaaaaag ccttgattaa ccgcaccact
     4501 ttttaccgtt attattcggg cgaaagtgat ttggtcggca aattgataaa agaggtgaag
     4561 gcggaatatc aagacctgct caaaaagcgt tttgaaagcg gtaatttgct gcgatttatg
     4621 caaaccattg gcaaccgctt attggaaaaa agacggctga ttttggcact ttggcaaatc
     4681 aaaacccctc gccatcattt gtatgacgat atgcacatgc tgttaaaaaa cgcctttatc
     4741 gcccacgcca aacggataac atcagatgat aaaaactggg ctgagaaaaa ttgggattac
     4801 caagcccata tttttgccac cattgccctt gaaagccata aatattattt tgaacaaggc
     4861 aaacttttgc ccattccaca ggtgtttgag gagtggatag aaatggtgca ggtgttgaaa
     4921 agtgtttaga aataactggt aaaattttcc ataaaaaaca ccgctttcat cacaaaagcg
     4981 gtgctttcaa atcatcaaac cttaaccaca atcttaccaa tctgctgatt gcttgccata
     5041 tattcgtgag ctttaaccat ttcatcaaag gcaaagattt tgtcaatttc agggcgtaac
     5101 tgaccgcttg ccaaaccgtc atagacaaag gttttggctt gtgccaattt ttcaggtacg
     5161 gtggtaattt caaagagttc atacccccgc acggtcaaat gcttacccaa aatcgggaac
     5221 actggcacag caatatcatc atggcttaac gctccataaa tgatgtaacg accatcttgt
     5281 gccattgcat tgatgatttt tgccgcctct tttctgccca cagggtcaaa taccacattg
     5341 acgcctttgc cattggtgat ttcaaaaagt ttggcggtta catcgtcttc gcttgtggca
     5401 atcacaaaat ccgctccttt ttccaaaagt acatcgcctt tggcgtgggt gcgagacaag
     5461 gcaatcacgg ttgccccttg cattttggcg atttggatac tggctaaacc aaccgaactg
     5521 gttgcaccgc ccagcaccac aaaatcgcct ttttgtactt tgccaaactc aatcagtccg
     5581 ccataagcgg taacaaacgc catccaactg gcggacgctt gttccattga caaattatca
     5641 gggtgcttga ccacagcgtg ttttggcata ttgacgattt cgccataagt gccgtattcg
     5701 gtaaacataa aggacggaat aatgctcacc ttatcgctaa tggcaacttc gttcacaccc
     5761 tcgccaatcg ccacaatcac accagcccct tcataaccca aagtcgcagg gaatacaggg
     5821 tcaatcacat acgccccttc acggtacatc atttcggcac ggtttaagcc aatggcttgg
     5881 atttggattt gtacttcgtg tgcttttggt gcaggaattg ctacatcaac gatttgcaag
     5941 acatcaggcg aacctgtttt tgtaaattgg atttgtttgc tcattgtttt gaccttttgt
     6001 gtaaataatg ggcgtatttt aatgcaatga atttatttta aacacaacat caattgcaaa
     6061 acacttttgc taaatttaca taaatacatt ttcacaatgt ctgaaaagtt gttttgcaaa
     6121 ttttaaaaag aaaaccaccg cttgccgatc acaagcggtg gtttttgtgg gttattttgc
     6181 aagaggtgtc agcttggcta cagtaagcgg ataaatgcga cgccggctaa gagtaaggta
     6241 acgccgatga tctgcagcgg tagaatgggt ttttgtgtaa tgccgaacag accgaattta
     6301 tctaccagca ggctgccgca aatcaggcca gagaggacga gcatcaccac cgagcctgtg
     6361 ccgatttggg ggacgagcca aacgccagcg aggataaaca ggctgcccag tatgccgccg
     6421 ctccatatcc aaaacggttt gccttcggtt ggctggcaga gtttcggcag gcttttttca
     6481 taaaacagca ctaccagtaa taagccgatg gcaccgacac cgaaggagac tgttgctgcg
     6541 ccgagtgatg agccgggctg tctgccgaat tcgccattaa cggcggcttg ggttgctaat
     6601 gcggttccgc ctaatatgcc gactgcgcgc cacagccata aatttgcgtt ttctttgcgt
     6661 tttagaccgg acagatgggg cagtaccacg gcaatccata cgccgaataa gactaaacca
     6721 atgccaaaca ggcggctggc cgtgaaggct atgtaaggcg agttaagcca agcgaaagtg
     6781 tcgatcagca cgcccatcag cacttggcct aaaatcggca tgaccgcagt ttgtacgccg
     6841 ccgagcttgg ggaagatcag tacgtttgcc gttagcccca ccatgccgag taatccgccc
     6901 gtccatgccc ataaggggac gttgcctaaa ttgtcgaggc taaatgctgg tgtttgttgc
     6961 atgatgagcg agagtccgag tagaaacagg aggcctacgc tgaaagagac aaacgaggtt
     7021 aaaaatgccg cacccacgtg catacgcaaa cgggcgttga tggctgattg aaatgcaatc
     7081 agcacgccgg ctaaaatacc gataataaat gtcatgacta actgggttgt gctgccgcct
     7141 caaaatgagt ggtggttgaa aagaaatgcc gtctgaatgc ggattcagac ggccttaagg
     7201 cttggtatcg gcgtttacct gatgttgaat agccgttttg cctagccggt ttattcgatg
     7261 aatttcacca cggtttcgaa atctttacgt gctttttggc ggatttcttt atcacggtaa
     7321 ccgaaggtac acatcaccga tacgccccat tcgcttgggt caaataatcc ttccgctgcc
     7381 aaaatatcgt tgacttcttg gtaagcgaaa ccttctatcg gacaagaatc tacgccaatt
     7441 aaagctgcac cggtcatcat attgcccaat gcgatgtagg tttgtttgct tgcccagtca
     7501 aacaggctgc gttcgctgtc taaaatgccg atatctttct cttggaattt tttatacacg
     7561 gccatggctt tttcgcgcgc ttcgccttgt aaacctcgtc tatccataat ttcagaaaaa
     7621 tattggctgt cgtaacgggc atttttcttc gctaaaatca ccacgatatg gcttgaggtt
     7681 tccatagttg gaatgcccca gcagtaaggt ttgatttttt gccgtaattc agggttttgc
     7741 aacactaaaa actgccaagg ctctgagcct accgaacttg gacttaaacg accgagttcc
     7801 agaatatagt tgaaatcttc agccggaatt tttctgctgc cgtcgtaaga acgggtagat
     7861 gcacggtaat gaaaggcatt gattacgtct tctttgttga aaaattgcat ggaacactcc
     7921 taaaaatgca aaacagtctt ttcagacggt ctgaaaagtc tagatgaaat acggataagt
     7981 ataaaagatt taagttattt ttaataattg ataattttga tatttaggta taagttgacg
     8041 tgatgagaaa aaacagaccc tctgaatgtt caggcggtct ttaacgttgg ttgtttacat
     8101 aaataatccg cgttcatatt gcttcggtac aaactgcatt tcactgctgt ctgctgcatt
     8161 ttggtcatat tgcgcattca atacccagta cggatcgcgc aataaggctc ttcccacagc
     8221 cactaaatcc gcatcgccca tacctaagat atgatcgcct accgccggat tatccaacat
     8281 acctactgca attaccggtt tgcccgtagc gagcttcact tgacgggcaa agtctacttg
     8341 gtaacctgcg taaaattcgg ggtgattacc tgcatgcaat acaccatcac caccaccgct
     8401 gatgtctaag acatctgcgc ctgcatcggc aaaacgtttc gctacctgca aaccataatc
     8461 caagccaaag ccgttttcac cgtattcttg tgcagaaatg cggacaatta acggcatttc
     8521 agacggcatc acttcacgtg ctgcctgaat cacttgctcg ccaaacaaca ttttgtcttg
     8581 gccgtactca tcactgcgct gattggattt cggcgaatag aattggtgaa ttagataacc
     8641 gtgtgcgccg tggatttcaa ttgtatcaaa gcctgcctca acggcacgtt tgaccgaatc
     8701 cttaaaggct tgaaccaatg ctttgacttc gtctgtcgtc agctcgcgag gtgtaatcag
     8761 attttgtccg gcataatcca actcaccgta gtgaattgca cttggagcca ctgcatccgg
     8821 ctcgtcttgg gctttgcgtc cggcatgggc gatttgcaca ccgatttttg ccccttgagc
     8881 atgaacgctg tctaccagtt ttttgaatgc ctgttgttgt tcatcgttcc ataagcctaa
     8941 gcagtttggc gtaattctgc cgtttggcgc cacattggtc atctccacaa taatcaagcc
     9001 gacaccacct actgcacggc tggtgtaatg cacgaaatgc caatcgttcg gtacgccgtt
     9061 ggttgccgaa tattgacaca ttggtggcat aaccacacgg tttttcaatg ctaagttttt
     9121 aatgttaaac ggggtgaata attttctgaa cttcgccatg ttgccttcct taatgaaata
     9181 gattaggtgg aatgataatt cagtgctatt atctattcaa attgattatt gtgatagtta
     9241 ggtataaaaa tgagtgatat aagaacactg gatttcaatt tgctcaaagc ttttgtggtg
     9301 ctgctggacg aatgcaacgt tagccgggca gcacaacgat tatccatcac ccaaccggca
     9361 atgagcggta ttttaaaccg cttgcgcgag agttttaacg atccgttatt tgtgcgagtg
     9421 caacacggta tgcagccaac cgaccgcgcg ttgcaactgg ggcaaaccgc acgcaacatt
     9481 ctgcaagata tcagctccat gctgcaacca cccatattgg aaccggaaca tctgaatatg
     9541 accttgcgta ttgccgcaat ggattatgta caacaaatta ttgccttgcc gctgattctg
     9601 cgtttaagac ggcttgctcc gaatgtaaaa gtcgccttac tgccggtgca ggggcaaaat
     9661 atcaaaactt tgttcgagca aaaaaaaatc gatcttgcct tggtcagccg ccaacattta
     9721 agcacggata cgctgaaaac ggtattatat gaagaacgct acgtatgcgc gatgagcaaa
     9781 acacacccga tggcgcaaac accgctatcg ctcgatcaat tctgcaatct tcccttcgca
     9841 atgctttctt acaacggcgg agaattcagc ggagctacgg atctagcgtt acaaaagata
     9901 ggactacagc gcaaagtaat ggtctcggta aaccatattt ccctgctgcc ccagctgcta
     9961 caaggttccg acttagtcgc agtattgccc gaacgcttag caaaaacctt acccaatgtt
    10021 tatttacaac ctccgcctat agaggtagaa ggcttcacca tgatgatggc ttggcacgaa
    10081 agaaccgaac aagatacggc gcatcgatgg ttaagagagg tgctgcaggg tgtggtaaaa
    10141 cctaaacagg ctctttaatt attctttctt ttgctatgaa aaaagccgtt tatattttta
    10201 gataaacggc ttttttgcat tctaatatta gtatttttct acaaactgag cataggcatt
    10261 aatgaagttt tgcattgagc tttttaatgt ctcagaggca atttcaccct gttcatcaaa
    10321 tacgcctgta aataagtttc ctacgtttaa tgcgaaaggt aaggttggca tatttacaaa
    10381 tgagcctgaa gtaatagtac gtacttgatc agctgcacgt acaccgcctg ccgggcttgc
    10441 gccaacggtt acgatacctg ccggtttgcc aatccatttg ctttgcccca ttgggcgaga
    10501 aaccacgtca atcgcatttt taagcattgc agaaaagctt gcattatgct caggtgtgac
    10561 taataacacg ccatctgcca cagcaaccgc ttcacgcact ctggtgtatt gtgccgggct
    10621 gttttcatct aaatcacggt cgtaaagtgg taagtctgca atatccacaa tgtttaattg
    10681 aatgcttgca ggtgccattt tttgtaagtg acgaacagtc aagtgactaa atgaagtttt
    10741 gctgccgcta ccaattaata ctgcaatttg ttttgccatt ttataattcc ttagtacata
    10801 gagaatatag gcattttatc cctttcaatc tgtttcataa
//
LOCUS       DANLXF010000072        10805 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.072, whole genome shotgun sequence.
ACCESSION   DANLXF010000072 DANLXF010000000
VERSION     DANLXF010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10805
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.072"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            102..1994
                     /gene="mnmG"
                     /locus_tag="PXD66_001209"
     CDS             102..1994
                     /gene="mnmG"
                     /locus_tag="PXD66_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDL6319519.1"
                     /translation="MIYNQIYDVIVVGGGHAGTEAALAPARMGLKTLLLTHNVDTLGQ
                     MSCNPAIGGIGKGHLVKEIDAMGGLMAKATDVAGIQFRTLNSSKGPAVRATRAQADRV
                     LYRNAVRTALENQPNLDIFQQEVVDILVENNRAVGAVTKMGLTFKARAVVLTAGTFLA
                     GKIHIGLDNYAGGRAGDPAATMLADRLRDLNLRVDRLKTGTPPRLDARTINFDVLAKQ
                     HGDEVLPVFSFMGDVSQHPRQIPCYITHTNDQTHDLIRNSLDRSPMYTGIIEGVGPRY
                     CPSIEDKVMRFADRNSHQIYLEPEGLNTIEVYPNGISTSLPFDVQMGIVNSMKGLENT
                     RIIKPGYAIEYDYFDPRDLKPTLETKAIDGLFFAGQINGTTGYEEAGAQGLLAGINAA
                     LQVQGKEAWYPTRDQAYTGVLVDDLCTLGTKEPYRVFTSRAEYRLLLREDNADIRLTP
                     KAHELGLIDEARWVRFNQKMEAIEQERARLKSTWAHLQMPNLDELNALLANPLAREAS
                     GEDLIRRPEMSYEKLTQIALFAPAIEDKQAAEQVEIAIKYQGYIEHQYNEIERHKRHE
                     NTLIPVEFDYDKVESLSNEVRAKLMQHRPVSIGQASRISGITPAAISILLVNLKKQGM
                     LKRGEL"
     gene            2089..2313
                     /locus_tag="PXD66_001210"
     CDS             2089..2313
                     /locus_tag="PXD66_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319520.1"
                     /translation="MIYLRKLGFSPFDHQMFAQPSWYFCNALVHTNYENLRQGVFVTK
                     AYLVMFLENLLFEKSHSLSNRALRINENND"
     gene            2326..2940
                     /gene="rsmG"
                     /locus_tag="PXD66_001211"
     CDS             2326..2940
                     /gene="rsmG"
                     /locus_tag="PXD66_001211"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDL6319521.1"
                     /translation="MLAKLNRLLAQAEINLTDQQKEQLVGFVRLLDKWNKAYNLTSVR
                     NPDEMLVKHILDSLVVSRHLQGETFIDVGTGPGLPGIPLAIANPDKQFVLLDSLGKRI
                     TFIKNALRELGIKNVTPVQSRVEEYTESQFDGVLSRAFASLNDMIDWCYHLPTENGKF
                     YALKGVYDEQEIIEIKQPIQLVEVIKLEVPELVGERHLVVLKKP"
     gene            3208..4130
                     /locus_tag="PXD66_001212"
                     /pseudo
     CDS             3208..4130
                     /locus_tag="PXD66_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248294.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            4153..5199
                     /locus_tag="PXD66_001213"
     CDS             4153..5199
                     /locus_tag="PXD66_001213"
                     /inference="COORDINATES: protein motif:HMM:NF016497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDL6319522.1"
                     /translation="MQIQNPVLPGFNPDPSIVRVGDTYYIATSTFEWFPGVRIHESKD
                     LVHWNLVKNVLDTTTLLDMRGNPSSGGIWAPDLSYAEGKFWLVYTDVKITDGAFKDMT
                     NYLTTAKEIQGPWSAPIKLNGIGFDASLFHDDDGRKYLVQQTWDHREYHHPFDGITLT
                     EFDVATMKLKPETQRTIFEGTEVKLVEGPHIYKINGEYYLFAAEGGTVFTHQEVVARS
                     KTLEAHSFQLAPNTPFITNFDTPNSYLQKQGHGSLVDTPSGEWYYASLTARPWNHATE
                     SATDPRGWSTLGREAAIQKVEFDQDGWPYIVGGHGGKTFVEAPKDAIFTEAPIDHSQH
                     DEFDSSTLDINWNT"
     gene            5202..5840
                     /locus_tag="PXD66_001214"
     CDS             5202..5840
                     /locus_tag="PXD66_001214"
                     /inference="COORDINATES: protein motif:HMM:NF036826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,4-xylosidase"
                     /protein_id="HDL6319523.1"
                     /translation="MPFSKEMGEVGNGVLKLIGRGSLANTHDLSLIARRWQAFYFDAE
                     TKVKFTPFSYQSMAGLTNYYNHSHWSWIFITKNDQGQQVIEVAENKGGLRNGQYTSYL
                     KDKAIVIPDGTEYVWFKTKVRKETYRYEYSFDGKQWHTIPVELDAAVLSDDYVLRSYG
                     GFFTGAFVGLAAVDYSSYESTAEFSYFDYKELGDKQLTDGSFSWEKSESCFG"
     gene            5859..6839
                     /locus_tag="PXD66_001215"
     CDS             5859..6839
                     /locus_tag="PXD66_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL6319524.1"
                     /translation="MKQRRFGKTNKMVSEIGLGTWQLGTKWGEPFNHQEAMAILETAY
                     EQGINFIDTADVYNNGQSEKAIGDILKKYPDFFYVVTKCGRALNPHTAEMYTPQAIEK
                     FVDGSLSHLGTEKLDMILLHCPPTLVYRNDEIFTKLEQLKAGGKLIDYGVSIETVEEG
                     LLAMEYDIAAMEVIFNMFRLKPLEQLFPTAQQKNVGIIARVLLASGLLTGRYNACTQF
                     GKDDHRTFNRNGEAFDKGETFSGVDYQLGLQAVEELKALFGTEDLIPYALRWVLMNEA
                     VSTVIPGASKVSQVITNAAVDNFPPLTENEMQKVEDIYNRLIRPSVHHLW"
     gene            complement(6901..8013)
                     /locus_tag="PXD66_001216"
     CDS             complement(6901..8013)
                     /locus_tag="PXD66_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL6319525.1"
                     /translation="MFSFLKASPPAEQKVEASRVDAEYRKLRWQVFAGVFIGYAAYYL
                     IRKNFSLAMPYLIDEYGFTKADLGTVGVALSLAYGFSKFIMGNVSDRSNPKYFITIGL
                     LSSAIVSLVFGLVPGVLSSIPIMIILAALNGWFQGMGYPPGAKTMTNWFSTSERGVWW
                     SWWNVSHNLGGGLIGPLAILGLSIFGVWQSLFYLPALIAIVLAFVMFYLMRDTPESQG
                     LPPIDEYKGEKVVEKVESAHTLSSKDIFYKYILNNKFLWAIAIVVYWKNPAGNPLIDN
                     ICLVAIGFLIYGPVMMIGLQAADLVPRVATGTATGLTGLFGYLLGSASAGYVMGKLVD
                     MFGWDGGFYALILSCFLAFAFIALTLFKKTSKQLEV"
     gene            complement(8203..9276)
                     /gene="glpQ"
                     /locus_tag="PXD66_001217"
     CDS             complement(8203..9276)
                     /gene="glpQ"
                     /locus_tag="PXD66_001217"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDL6319526.1"
                     /translation="MKFKFKTLVAALALSATALGAYAQQSDKLVIAHRGASGYLPEHT
                     LESKALAFGQQADYLEQDLAMTKDNRLIVIHDHFLDGLTDVAKKFPERKRADGRYYVV
                     DFTLDEIKTLEMTENFEVKDGKQAQVYPNRFPMWQSHFTIHTLEEELEFIQGLEKSTG
                     KKIGIYPEIKAPWLHHKEGKDIALETLKVLKKYGYDSKTSPIYLQTFDFNELKRIKTE
                     LLPKLGMDVKLVQLVAYTDWHETEEKDKNGNWVNYDYDWMFKDGAMAEVAKYADGVGP
                     GWYMLVDKEKSKVGNIVYTPLVKELAKYKMELHPYTVRKDALPDFFQNVDEMYDALLN
                     KAGATGVFTDFPDTGVEFLKKQK"
     gene            complement(9346..10791)
                     /gene="glpT"
                     /locus_tag="PXD66_001218"
     CDS             complement(9346..10791)
                     /gene="glpT"
                     /locus_tag="PXD66_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HDL6319527.1"
                     /translation="MFGPFKPAPHIAELPADKVDARYKFLRWQVFAGIFFGYAAYYFV
                     RANFDLAQKGLIEAGMYTKTDLGVIGTAAGLAYGLSKFVMAGMSDRSNPKVFLPTGLL
                     LSGLCMTLMGLMPWATSSILVMWVMIFLNGWFQGMGWPPCGRTMVHWWSKSERGTIVS
                     IWNTAHNLGGMVPGAMVLLASALYFSEYGVEATAKDVWQQALYFPGIAAMIAAIPIYL
                     VMKDTPQSCGLPPVEKWRNDYPDDYNEKTYEHDLSTKEIFVTYVLKNKLLWYIAIANV
                     FVYLIRYGVLKWSPVYLGEVKHFNIKGTAWAYTIYELAAIPGTLLCGWVSDKLFKGKR
                     GLTGFIFMILTTAAVVAFWLNPATPESEIAQYAGHAWYENPYQLMDFIWMTTIGFLIY
                     GPVMLIGLHALELAPKKAAGTAAGFTGLFGYLGGTVAASAVVGWAADKFGWDGGFYVM
                     IGGGVLAILLTFIVMVEEGKHKAKLEDHYGK"
BASE COUNT         3115 a         2279 c         2339 g         3072 t
ORIGIN      
        1 gatcctagcg ttgctaaaaa tctatttccc tgatgatccc tttttacgct aaaatatgcg
       61 tttactttgt agcagaattt atttgaataa aaaggcgata tatgatttat aaccaaattt
      121 atgacgtgat cgttgttggt ggcggtcacg ccggaactga agcagcactt gctcccgctc
      181 gaatgggctt aaaaaccctg cttttaaccc ataatgtaga taccttagga caaatgtctt
      241 gtaacccagc catcggtggg atcggtaaag gtcatctggt taaagagatc gatgcaatgg
      301 gcggtttaat ggcaaaagcc actgatgttg ccgggatcca attccgtact ctaaacagct
      361 cgaaagggcc tgcagtgcgt gctactcgag cacaggcaga ccgtgtgctt taccgcaatg
      421 cagtgcgtac agcattagaa aatcaaccga atttagatat tttccagcaa gaagtggtcg
      481 atattttggt ggaaaacaac cgtgcagtgg gtgcagttac caaaatgggc ttaacgttta
      541 aagcccgtgc ggtagtttta accgctggta cgttcttagc gggtaagatc cacattgggt
      601 tggataacta tgcaggtggt cgtgccggcg atcctgctgc aacaatgttg gcagatcgtc
      661 tgcgtgatct gaatttgcgt gttgatcgcc ttaaaacagg tacaccacct cgcctcgatg
      721 cccgtaccat caactttgac gtacttgcca aacagcacgg cgatgaggtg ttacctgtct
      781 tctcttttat gggcgatgtc tcacagcacc cacgccaaat tccgtgctac atcacccaca
      841 ctaacgatca aacccacgat ttaattcgca acagtcttga tcgcagcccg atgtacaccg
      901 gtattatcga aggggttggt ccacgttact gcccgtctat tgaagataaa gtaatgcgtt
      961 ttgccgatcg taatagccac cagatctacc ttgaaccgga aggcttaaat acgattgagg
     1021 tttacccgaa cgggatctcc accagcttgc cgtttgatgt gcaaatgggg atcgtcaact
     1081 caatgaaagg cttggaaaac acccgcatca tcaaaccggg ctatgcgatt gagtacgact
     1141 acttcgaccc gcgtgattta aaaccaaccc ttgaaaccaa agcgattgac ggtttattct
     1201 ttgccggaca aattaacggc acgaccggtt acgaagaggc aggtgcgcaa ggtttgctgg
     1261 cagggattaa cgcagcgtta caggttcaag gtaaagaggc gtggtatcca acccgtgatc
     1321 aagcctacac cggcgtgttg gtggacgacc tctgtacatt aggcaccaaa gagccatacc
     1381 gtgtgtttac ctctcgtgcc gaataccgtc tgttgctgcg tgaagacaat gcggacattc
     1441 gtttaacacc aaaagcccac gaactaggat taattgacga ggcacgttgg gtgcgtttca
     1501 accaaaaaat ggaagcgatt gagcaagaaa gagctcgttt gaaatccact tgggcacatt
     1561 tgcaaatgcc aaatctggac gaattgaacg cgttattggc taatccttta gcccgtgaag
     1621 caagcggtga agacttaatc cgccgtcctg aaatgagtta cgaaaagctg acccaaatcg
     1681 ccctatttgc ccctgctatt gaagacaagc aagcggcaga acaggtggaa attgcgatca
     1741 aatatcaggg ctatattgag catcaataca acgaaatcga acgccataaa cgccacgaaa
     1801 acaccctgat tccggtagag tttgactacg acaaagtcga aagcctctcc aacgaagtgc
     1861 gagctaaatt gatgcaacac cgccctgtct ccatcgggca ggcaagccgc atttccggca
     1921 tcacaccggc agcgatttcc attctgttgg tgaatttgaa gaagcaagga atgttgaaac
     1981 ggggtgagct gtaaatacta caagcggttg gttttgcaga aatttttgca aaattgaccg
     2041 cttgtattct ttggagaagg aaatacccga actaccgccg tatttttaat gatatatctg
     2101 cgtaaactcg gtttttcgcc gttcgatcac caaatgtttg cccaaccttc gtggtatttc
     2161 tgcaatgcac ttgttcatac caattatgaa aatctgcgac aaggtgtatt tgtgactaaa
     2221 gcgtatttag tgatgtttct tgaaaattta ttgtttgaaa aaagtcattc gttaagtaat
     2281 cgggcattgc gtattaatga aaataatgat taaggaattt gcaaaatgtt agctaaatta
     2341 aaccgcttgc ttgctcaagc cgaaataaat ttgaccgatc aacaaaaaga gcagttagtt
     2401 ggttttgtga gattattgga taaatggaat aaggcgtata acctcacttc cgttcgcaac
     2461 cctgatgaaa tgttggttaa acatattttg gacagccttg ttgttagtcg tcatttacaa
     2521 ggcgaaacgt ttattgatgt cggcacgggg ccggggctac cgggcattcc gctggcgatt
     2581 gctaaccctg ataaacagtt cgtgctacta gacagcttag gcaaacgcat cacttttatc
     2641 aaaaatgcgt tgcgtgagct tggtattaaa aacgttaccc cagtgcaaag tcgagtggag
     2701 gaatacactg aaagccagtt tgacggcgta ttaagccgag cctttgcctc gcttaatgat
     2761 atgatcgact ggtgttatca tttaccgact gaaaacggta aattttatgc attaaaaggc
     2821 gtgtatgatg agcaggaaat aattgaaatc aaacaaccga ttcagctagt ggaagtgatt
     2881 aagcttgaag tacctgagtt ggtaggagaa cggcatttag tagtgttgaa aaagccctaa
     2941 tacattggaa gtttaatgat acaagcggtc gaatttgtaa atttttttgc aaatctgcct
     3001 gcttgtgtta actatttttc ataaagcata cctggttaat aaggtgtgct ttttttataa
     3061 cctattattt catctgattt atttctattt tgtgatgtgc ttcacaaaaa caaaaaaaca
     3121 taatgtagta aaaaatattc gtaattgaag cccttatttc tttacctgaa aataagcgaa
     3181 cttaattata taacgaggag gttcgtaatg aacagggtaa caaataaggc agatgagttt
     3241 gctaaactca gtacgtttga acgtattgct tatggtagtg gcgacttagc ccaaaattta
     3301 gtttttggta cagttggtgg tttcttactc acttacttaa ctactgtaaa cggcatttct
     3361 gccgtagcag gagcaacgat tttcttaatt gtaaaatggg gaaatgtgtt ttgggatccg
     3421 tttgtcggtg cctttattga caaaagcaag ccctcagcac agggtaaata ccgcccgtat
     3481 ttactgaaat ttgggattcc tcttgtggtt ttagcttcac tactatttct tcctcttacc
     3541 taaaatataa tattgggcat tctgagtgga ttgcctccgt cggtttatta ggctctatcc
     3601 ctgggatttt ccttgtattc ttatggccga aactacgtcg cactatcggt aaaaagaact
     3661 ttttctatct atttttaggg attttcgttc tagggcaact ttgtctatgg gtatggcaat
     3721 ttgactcctt caaatactct ccaaccttag gctatatcgg tcgtttctta caacaatggg
     3781 gattaacttc tgctaccggt tttatgtggg cgttagtgcc tgaggttgtt acctttggag
     3841 agtttaagtc gaaaaaacgt gtagcaggta ttattaatgc gttaatgggc ttgttcttta
     3901 aaatcggctt ggcgttaggc ggtattattc cgggttatat taatgcggtt tatggtttta
     3961 acggctcgct tttggctcaa accgaagagg ctttatttgg tatcaatctt tcaatgattt
     4021 gggctccaat tattttagcg attattgcag gatttgtgat gagtcgctat ccgttaagcg
     4081 atactgatat tgatcatatc aacgaaaaaa ttactgctta ttccaaataa tttgagtata
     4141 ggagaataga ttatgcaaat tcaaaatcca gttttacccg gtttcaatcc agatccatct
     4201 attgtgcgtg tgggtgatac ttactacatt gcaacttcta cttttgaatg gtttccggga
     4261 gtgagaattc acgaatcgaa agatttagtg cattggaatt tagttaaaaa tgtcttagat
     4321 acaaccacgt tgttagatat gcgaggcaat ccctcttcag gtggtatctg ggctcctgat
     4381 ctttcttatg cggaaggtaa attttggttg gtttataccg atgtaaaaat tacagacggt
     4441 gcattcaaag atatgactaa ctatctaact actgccaaag aaattcaagg tccttggtca
     4501 gctccgatta aattgaatgg tattgggttt gatgcttcgt tgtttcacga tgatgatggg
     4561 cgaaaatatt tagtgcaaca gacttgggat caccgtgaat atcatcaccc ctttgacggt
     4621 attacgttaa cggaatttga tgttgccaca atgaaactca aacctgaaac ccagcgtacg
     4681 atttttgaag gtacagaagt gaaattggtg gaaggtccac atatttataa aattaatggc
     4741 gaatattatc tgtttgcggc ggaaggcggc acagtattta cgcatcaaga agtagtggct
     4801 cgttctaaaa cattggaggc tcactctttc caactggctc caaatacacc gtttattact
     4861 aattttgata cacctaattc ttacctacaa aaacaagggc acggttcatt agttgatacc
     4921 ccaagtggtg aatggtatta cgcttccctc acagcaagac cttggaatca tgcaacagaa
     4981 tccgcaaccg atcctcgtgg ttggtcaaca ttagggcgtg aagccgccat tcaaaaagta
     5041 gaatttgatc aagacggctg gccttatatt gtgggtggac acggtggaaa aacctttgta
     5101 gaagccccaa aagatgcaat ttttaccgaa gctcctattg atcattctca acacgatgag
     5161 tttgatagct caacgttaga tattaactgg aacacttaag agtgcctttt agtaaagaga
     5221 tgggcgaagt tggtaatggt gttttaaaat tgattgggcg aggctctctt gcaaatacac
     5281 acgatttatc gctaatcgca cgccgttggc aagccttcta ttttgatgcg gaaaccaaag
     5341 tgaaatttac accgttttct tatcaatcta tggcaggttt aactaactat tacaatcact
     5401 cacactggtc ttggattttc atcaccaaaa atgatcaagg acaacaggtg attgaagttg
     5461 cagaaaataa aggtggactc agaaatgggc aatatacttc ttaccttaaa gataaagcaa
     5521 ttgtcattcc tgatggaacg gagtatgttt ggtttaagac taaagttcgg aaagaaactt
     5581 accgttatga atattcattc gatggcaaac aatggcacac tattccggtt gaattagatg
     5641 cggcagtgct ttcagatgat tatgtgttac gcagttatgg cggtttcttc acaggagctt
     5701 ttgtcgggct ggctgcagtg gattactcca gttatgaatc tacagcagaa tttagctact
     5761 ttgactataa agagttaggc gataagcaac ttacagacgg ctcattctct tgggaaaaat
     5821 ctgaaagttg ctttggctaa aatgaggaga atatcacaat gaaacaacgc agatttggta
     5881 aaaccaacaa aatggtgtca gaaattgggt taggcacttg gcagcttggg acgaaatggg
     5941 gtgaaccgtt caatcatcaa gaggcgatgg caattttaga aaccgcttat gagcaaggca
     6001 tcaattttat tgataccgct gatgtgtata acaacgggca aagcgagaaa gccattggtg
     6061 atattttgaa aaaataccct gactttttct atgtcgttac caaatgtgga cgtgcgttaa
     6121 acccgcatac tgctgaaatg tacacgccac aggcaatcga aaaatttgtt gatggtagtt
     6181 taagccatct tggcacggaa aagttggata tgattttgct ccactgcccg ccaacgttgg
     6241 tttatcgcaa tgatgagatt tttacgaaat tggaacagct taaagccggc ggaaaattaa
     6301 tagactacgg tgtgagtatc gaaacggtag aggaaggctt gttggcaatg gagtatgaca
     6361 ttgcggcaat ggaagttatc ttcaatatgt tccgcttaaa accattggag caactcttcc
     6421 caaccgctca gcaaaaaaat gtgggtatta tcgcccgtgt actgttggca agtggtttgc
     6481 ttacaggcag atataatgcc tgcacccaat ttggcaaaga cgaccaccgc acatttaacc
     6541 gcaatggtga agccttcgat aaaggcgaaa ccttttcggg tgtggattat caactcggtt
     6601 tacaagcggt ggaagaattg aaagccctat tcggcacgga agatttaatt ccttacgccc
     6661 tacgttgggt attgatgaat gaagctgtga gtacagtaat cccgggagca agtaaggttt
     6721 cacaagtaat tactaacgca gcggtggata atttccctcc actcactgaa aatgaaatgc
     6781 aaaaagtgga agatatttac aaccgcttaa ttcgcccaag tgtgcatcat ttgtggtaat
     6841 ttatccaacg ctataaaaaa tggaactcta acttgagttc catttttgtc aattttagcc
     6901 ttaaacctct aactgtttag aggttttctt aaacagagtc aatgcaataa acgcaaaggc
     6961 taagaaacag gaaaggatca gggcatagaa accaccgtcc cagccgaaca tatcaaccag
     7021 tttacccatc acataacctg cacttgcaga gcctaacaag tagccgaata aaccggttaa
     7081 acctgttgct gtaccggtgg caacacgagg cactaaatcc gctgcttgta aaccgatcat
     7141 catcaccggc ccgtagatta agaaaccaat cgccactaag cagatgttgt cgattaacgg
     7201 gttgccggct gggtttttcc agtacaccac aatcgcaatt gcccacagga atttgttgtt
     7261 taaaatatat ttgtagaaaa tgtctttgga agagagtgtg tgggcagact ccactttttc
     7321 caccactttc tcgcctttgt actcatcaat cggcggtaag ccttgagatt ccggcgtatc
     7381 acgcatcaaa tagaacatca caaacgccag tacaatcgca atcaacgccg gcagatagaa
     7441 cagggattgc cacacgccaa aaatagataa gcctaaaatc gccaacggcc cgattaaacc
     7501 gccgcccaag ttgtgcgaca cgttccacca gctccaccac acgccacgtt cagaggttga
     7561 gaaccagttg gtcatcgttt tcgcccccgg cggatagccc attccttgga accagccgtt
     7621 taacgccgcc aaaatgatca taattggaat agacgaaagc acgcccggta ctaaaccgaa
     7681 caccagactc acaatcgctg aactgagcaa gccgatagtg ataaagtatt tcgggttaga
     7741 acggtcggaa acgttaccca taataaattt actgaagccg taagcgagtg ataatgccac
     7801 gcccaccgta cctaagtctg ctttggtgaa gccatattca tcaatcaaat acggcattgc
     7861 caacgagaag tttttacgaa ttaaataata tgccgcatag ccgataaaga cgcctgcaaa
     7921 cacttgccaa cgtaatttgc gatattccgc atcaactctg ctggcttcca ctttttgttc
     7981 tgctggtgga gaggctttta gaaaggaaaa cattttacta ctcctttaag aatgaaatcc
     8041 tatggctact ataatctttc atttggagca gaaaaagtgg atttttgaaa atttgtgagc
     8101 aagattaaaa ttattttgag cgaaaacgaa aagcggttga atttccttct ttttttgcaa
     8161 aaaatttgag aaatctaacc gcttgtttca cttaaaaatc agctatttct gctttttcaa
     8221 aaattccacg cccgtatcag ggaagtcagt aaagacgcct gttgcacccg ctttattcag
     8281 cagggcatcg tacatttcat cgacattttg gaaaaagtcc ggcagagcat ctttgcgcac
     8341 ggtgtatggg tgaagttcca ttttgtactt cgccaactct ttcactaatg gcgtgtaaac
     8401 aatgttgccg actttagatt tttctttatc caccaacata taccagcccg ggccgacacc
     8461 atcggcatat tttgccactt ctgccatcgc accgtctttg aacatccagt cgtagtcgta
     8521 attaacccag ttgccgtttt tatctttctc ttcggtttcg tgccaatcgg tgtaggcaac
     8581 taactgcacc aattttacgt ccatacccag tttcggtagt aattcggttt tgatccgttt
     8641 cagctcgttg aaatcgaagg tttgcaggta aattggtgag gttttgctgt cgtagccata
     8701 ttttttcaac actttcaggg tttcgagtgc aatgtcttta ccctctttat ggtgcaacca
     8761 aggggcttta atttcaggat agatcccaat ttttttgccg gttgatttct ctaatccttg
     8821 aataaattcc agctcttctt ccaaagtgtg aatggtgaaa tgggactgcc acatcgggaa
     8881 gcggttcggg taaacttgtg cctgcttgcc gtctttcacc tcaaaattct cggtcatttc
     8941 aagggtttta atctcatcaa gggtgaaatc caccacataa taacgaccgt ctgctcgttt
     9001 acgctcaggg aattttttcg ccacatcagt taaaccgtct aaaaagtggt cgtgaatcac
     9061 aatcaggcgg ttatctttgg tcatagcaag atcttgctct aaatagtccg cttgttgccc
     9121 gaatgctaga gctttagatt caagcgtatg ctcaggtaaa taaccgcttg caccacggtg
     9181 ggcaatcact aatttatcag attgttgtgc ataggcaccc aatgctgtcg cggacaatgc
     9241 taatgcagca accaatgttt tgaatttaaa tttcatagaa ttacctcata taatgtaaaa
     9301 ataaaaggaa gaacaaaaat gtccttcctt ttgataagtt atttattatt taccatagtg
     9361 atcttctaat tttgctttgt gcttgccttc ttcaaccatc acaatgaagg ttaagagaat
     9421 ggctaataca ccaccaccga tcatcacgta gaaaccgccg tcccagccaa atttatctgc
     9481 tgcccagcca actactgcag aggctgccac tgtaccacct aagtaaccga ataaaccggt
     9541 gaaacctgct gctgtacctg ctgctttttt cggtgcaagt tcaagggcgt gtaagccaat
     9601 caacattacc gggccgtaga ttaagaaacc gatagtggtc atccaaatga agtccattaa
     9661 ttgatatggg ttttcatacc aagcatggcc tgcatattgt gcaatttcag attccggtgt
     9721 cgcagggttg agccagaacg caaccactgc tgcggtggta agaatcataa agatgaaacc
     9781 ggttaaacca cgtttgcctt tgaacagttt gtcagagacc caaccacaga gtaatgtacc
     9841 tggaatcgca gctaattcat aaatggtata tgcccatgct gtacctttaa tgttgaagtg
     9901 ttttacttcg cctaagtaca ccggcgacca tttcaatacg ccatagcgga ttaagtaaac
     9961 gaagacattc gcaatggcaa tgtaccataa cagtttgttt ttcagcacat aagtcacgaa
    10021 aatctctttg gtgcttaaat cgtgttcata ggttttttcg ttatagtcgt cagggtagtc
    10081 gttacgccat ttttcaactg gcggtaaacc gcaagattgt ggagtatctt tcatcacaag
    10141 gtagattgga atagctgcga tcattgctgc aattcccggg aaataaaggg cttgttgcca
    10201 tacatctttt gccgttgctt ctacaccata ctcactgaaa tagagcgcac ttgctaatag
    10261 caccatcgcc cccggcacca taccgcctaa gttgtgagcg gtattccaga tagaaacgat
    10321 agtaccacgc tcagatttag accaccagtg aaccattgtt cgtccacacg gtggccaacc
    10381 cataccttga aaccagccgt ttaagaagat cattacccac attactaaaa tgcttgatgt
    10441 tgcccaaggc attaagccca taagggtcat acatagacct gaaagtaaca atccggtcgg
    10501 taaaaatact ttcgggttag agcggtcgga cattcctgcc atcacgaatt ttgataaacc
    10561 gtaggccagg ccggcagctg taccaatcac accgagatcg gtttttgtat acataccagc
    10621 ttcaatcaag cctttttgtg ctaagtcgaa gtttgcacgc acaaaatagt aagcggcata
    10681 gccgaagaaa attcctgcaa acacttgcca acgcaagaat ttatatcttg catcgacttt
    10741 gtccgccggt aattccgcaa tgtgcggagc gggtttaaat ggaccaaaca tagtattctc
    10801 caata
//
LOCUS       DANLXF010000073        10678 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.073, whole genome shotgun sequence.
ACCESSION   DANLXF010000073 DANLXF010000000
VERSION     DANLXF010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10678
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.073"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..848)
                     /gene="pdxY"
                     /locus_tag="PXD66_001219"
     CDS             complement(<1..848)
                     /gene="pdxY"
                     /locus_tag="PXD66_001219"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HDL6319528.1"
                     /translation="MKNILSIQSHVVYGYAGNKASTFPMQLLGVDVWALNTVQFSNHT
                     QYGKWGGMVMPKEQIGSIVDGIHNIGELHRCDAVLSGYIGSAEQVAEIIQAFHKVKAE
                     NPNAIYVCDPVMGHPDKGCIVADGVKEGLINLAMAQADIITPNLVELRELTGLTVENF
                     EQAVEAVKVILTKGPKKVLVKHLSRVGKESNKFEMLFANQDGIWHISRPLYQFAKEPV
                     GVGDLTAGLFVANLLNGKSDLEAFEHTANAVNEVMEQTARSGLYELQIIASRDRIVNP
                     QCSYQAV"
     gene            967..1042
                     /locus_tag="PXD66_001220"
     tRNA            967..1042
                     /locus_tag="PXD66_001220"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1000..1002,aa:Thr,seq:tgt)
     gene            complement(1137..2090)
                     /gene="accA"
                     /locus_tag="PXD66_001221"
     CDS             complement(1137..2090)
                     /gene="accA"
                     /locus_tag="PXD66_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDL6319529.1"
                     /translation="MSQEFLDFELPIAELEAKIESLRAVSEQDDKINLDDEIKRLQKK
                     SEELTKKTFANLDAWQISRMARHPNRPYTLDYIEHIFTEFDELAGDRAFADDKAIVGG
                     IARLDGRPVMVIGHQKGRTVKEKVKRNFGMPAPEGYRKALRLMEMAERFNMPIITFID
                     TPGAYPGIGAEERGQSEAIARNLREMSQLSVPVICTVIGEGGSGGALAIGVGDKVNML
                     QYSTYSVISPEGCASILWKSADKASTAAEVMGLTAPRLKELELIDNIVPEPLGGAHRN
                     VEQIAQNLKQRIVEDLAGLDQLNKEDLLERRYQRLMNYGYV"
     gene            complement(2144..3568)
                     /locus_tag="PXD66_001222"
     CDS             complement(2144..3568)
                     /locus_tag="PXD66_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HDL6319530.1"
                     /translation="MKKQITRRQFLQGSALAAGFATVPTPLLAAERPQLNIPPLMDIG
                     RGKPVRLDLRPAQTQFDKGKLVDVWGVNGQYLAPTVRVKSGDFVKLTYLNNLPQKISM
                     NIQGLQASSEMIGTVHRSLDPKSSWSPIVSINQPACTCWYHADTMLNSAFQVYRGLAG
                     LWMIEDEQSKKATLPNKYGVNDIPLILQDQLLNKDGVQVLDKNTSQFFGKRLFVNGQE
                     SPYFNVPRGWVRLRIVNASLSRAYELRLDNEQPLYLIATGLGMLAEPLETESVQLAPS
                     ERVEVLVDLSEGKAVSLISGQKRDIFYKIGQVFADNDELNDNVILELRPEGMAAVLNN
                     KPSLPAFNLEEFNLKISQERRFAIRPADYLINQKRFDPKRIDFTAQKGSVERWYLHST
                     QAVGFTLQGAKFIIETLNRKQYPIKQLAWRDTVWLEAEQEITLLVRFDNTAPEQLPFT
                     FGVSDFMLRDRGTMGQFSVIENGQ"
     gene            complement(3570..4304)
                     /locus_tag="PXD66_001223"
     CDS             complement(3570..4304)
                     /locus_tag="PXD66_001223"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HDL6319531.1"
                     /translation="MLKIFRILIVALAAIFISIFGTIYALLRLRHPSSVGVVARWFGA
                     LHKLVGLELITRKKPEINYPVVYIGNHQNNYDMVTISSMVAEKTVSIGKKSLIWIPFF
                     GLVYWATGNIFIHREKRSSAIETMNKAGQIVKERNVSVWMFPEGTRSRGRGLLPFKTG
                     AFHLAISAGVPIVPVVCSNLHNKVDLNRSNNGTVICEMLDPIDTSGYSRENVKELMEK
                     CHQIMLAKFEQLNQEVEQLENTAKNA"
     gene            4451..4948
                     /gene="tpx"
                     /locus_tag="PXD66_001224"
     CDS             4451..4948
                     /gene="tpx"
                     /locus_tag="PXD66_001224"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005578656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HDL6319532.1"
                     /translation="MSKVTLAGSPIDVAGTFPQKGDVVADFTLVNSDLSDVSLSDFDG
                     KRKVLNIFPSIDTGICATSVRVFNQKAADVANTVVLCISADLPFAQARFCGAEGIENA
                     KVLSSFRHRDLHNKLGVDIISGPLAGLTSRSVIVLDENNKVLHSELVPEIKQEPDYEA
                     ALAVL"
     gene            complement(5033..6658)
                     /gene="yidC"
                     /locus_tag="PXD66_001225"
     CDS             complement(5033..6658)
                     /gene="yidC"
                     /locus_tag="PXD66_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDL6319533.1"
                     /translation="MNNRGLLLMGLFLVSILIFTQWQQDFDPEIQAQKQAQVAAQTQQ
                     AGDLPSASNANNPIADSITQGKTITVESDVLRLTIDTLGGDVIGSDLLKHNAELHSNT
                     PFKLLQQDGDKIYVAQSGLVGKNGIDTNAGRPQYAVTADSFKLAEGQNEISVPLTFEK
                     EGVVYTKTFTLKRGSYDVGVNFNIKNNTAQAIEVQPYGQLKHSIVESSGNLTMPTYTG
                     GAYSSSETNYKKYSFQDMEKANLSVDTKAGWIAVLQHYFVSAWVPNQDADNSLYSRTN
                     NGVATIGYRGPLTTIAPNSEANLNSKLWTGSKDQKEMGESANHLDLTVDYGWAWFIAK
                     PLFWLLTAIQKIVSNWGLAIIGVTIVVKTILYPLTKAQYTSMAKMRMLQPRITEMRER
                     FGDDRQRMSQEMMKLYREEKVNPMGGCLPILIQMPIFIALYWTFMEAVELRHAPFFGW
                     IQDLSAQDPYYILPLLMGGSMFLLQKMSPTPVADPVQQKVMTFMPVIFTVFFLWFPSG
                     LVLYWLTSNLITIAQQWLIYRNLEKKGLHSRKK"
     gene            complement(6811..7236)
                     /locus_tag="PXD66_001226"
     CDS             complement(6811..7236)
                     /locus_tag="PXD66_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HDL6319534.1"
                     /translation="MTIWKQTATCEQLNTLSQKSAVSHLGIEFTAIGEDWIEAQLQVD
                     ERTQQPFGVLHGGVSAALAETTANAGALMVCEPHQIAVGMELNISHLKSIPAGTKAIA
                     KATPLKLGREVQVWNIDIKDEQGNLCAVARLSTKTLDKR"
     gene            complement(7565..10657)
                     /locus_tag="PXD66_001227"
     CDS             complement(7565..10657)
                     /locus_tag="PXD66_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDL6319535.1"
                     /translation="MIPRLSEVPQLDKATQSYLTELVNQHFSGEIASNYADRLSLATD
                     NSVYQQIPQAILFPKSVSDVVILTKLAQKPEYQHLTFTPRGGGTGTNGQSLNNNIIVD
                     LSRHMNQILELNVEERWVRVQAGVVKDQLNQFLKPHGLFFSPELSTSNRATLGGMINT
                     DASGQGSLQYGKTSDHILAIKSVLINGDILETQAVKSADFFAHIEGLNLSALGKTLHR
                     EIFNRCKTLRPKVLNELPQLNRFITGYDLKNVFNHGESEFNLTRILTGSEGSLAFICE
                     AKLNLLPIPKYRTLINIKYNSFDAALRSAPFMLEAKALSVETVDSKVLNLAKQDIVWH
                     SVSDLLTEDPANPILGINIVEYASSDQTQIETQVKALCQSLDQKMANNEAGIIGYQLT
                     SDVNSIEKIYAMRKKAVGLLGNAKGWAKPIAFVEDTAVPPENLADYIVEFRALLDRYN
                     LPYGMFGHVDAGVLHVRPALDLCDKAQVETFKQISDQVVELTAKYGGLIWGEHGKGMR
                     SYYGERFFGETLWQELRYIKTLFDPYNRLNPGKICTPLESETSLYPIDSQMRADFDRQ
                     IPIQVREEFKGAMNCNGNGLCFNFDVHSTMCPSMKVSGNRLYSPKGRATLVREWLRLL
                     TEQNVSPDDLIFKPRKQAVKLADFVAKIRNTLNKNKEYDFSHEVKAAMDTCLACKACA
                     SQCPIKIDVPTFRSQFNELYHSRYLRPLKDYVVSNLEFIAPLMAKQPRFFNFFSTSTF
                     AEKAANKTIGMTYLPALSVPNLQHQLVEVGYQGESLESLEQQATSGDFSAKNCKTLFI
                     VQDPFTSYYDAKVVADFIALIKKLGFKPIVLPFKPSGKAQHVKGFLHKFAKTAKNQAD
                     FLNRVAKLGLPMVAVDPALTLLYREEYKEILKEARGDFKVLLAHEWLREVIKNGELEN
                     CKKSANSDRLPWYLFAHCTESTALPSSMKEWQAIFTHFGETLLNENVGCCGMAGTFGH
                     ETKHVEMSKAIYQQSWQIKLKNKPLERCLATGYSCRSQVKRFEQQGIKHPIQALLDVI
                     "
BASE COUNT         2839 a         2285 c         2295 g         3259 t
ORIGIN      
        1 acagcttggt aactacactg tggattaaca atccgatcac gagaagcaat aatttgtaac
       61 tcataaagcc ctgatctagc ggtttgttcc attacctcat tgaccgcatt tgcagtgtgt
      121 tcaaaggctt ctagatcgga ttttccattt aataagttcg ccacaaatag ccctgcggtt
      181 aaatcgccta caccgacagg ttctttggca aattggtaga gtgggcggct gatgtgccaa
      241 atgccatctt ggttggcgaa cagcatttca aatttgttgc tctctttgcc gactcggctt
      301 aaatgtttaa ccaatacttt tttcgggcct ttggttaaaa tcactttcac tgcctcgacc
      361 gcttgctcaa aattttccac cgttaaaccg gttaattctc ttaattccac taaatttggt
      421 gtgataatgt cggcttgtgc cattgccaga ttgatcaatc cctcttttac gccgtctgcc
      481 acaatgcagc ctttgtcggg gtgtcccatt accgggtcgc atacataaat tgcgtttgga
      541 ttttccgctt tcactttatg aaaagcctga ataatttcgg ctacttgttc tgccgagccg
      601 atatagccag agagtacggc atcgcaacga tgcagttcac cgatattgtg aatgccatca
      661 acaatgctac cgatttgttc ttttggcatc accataccgc cccattttcc atattgggta
      721 tggttggaaa actgtacggt atttaacgcc catacatcaa cacctaacag ctgcatagga
      781 aaagttgaag ccttattgcc ggcatagccg tagacaacgt gcgattgtat ggatagtatg
      841 tttttcattc attcactcct ttaaaatttc aatatgtgtt gttttttagt catttgaagt
      901 tgcaaggact tgaagctcat caagaaatct ctataatccc gcctcgttct cttatccatt
      961 caatatgccg acttagctca gtaggtagag caactgactt gtaatcagta ggtcaccagt
     1021 tcgattccgg tagtcggcac catcaccttt attttctgat gagtcccctt taatgtaaaa
     1081 tggcaagatt ttgcaaattt tcacaaaaat cttaccgctt gtagggtgaa attgaattag
     1141 acgtaaccgt aattcattaa gcgttgataa cggcgttcca gtaaatcctc tttattaagt
     1201 tgatctaacc ctgccaaatc ttcaacaata cgctgtttaa ggttttgggc gatttgttca
     1261 acattgcgat gagcaccgcc taagggttcc ggcacgatgt tatcaatcag ctcaagctct
     1321 tttaaacgag gggcagttaa acccattact tctgctgccg ttgaggcttt atcggcactt
     1381 ttccacagaa tcgaggcaca gccttcaggt gaaattaccg agtaagttga atattgcaac
     1441 atattgacct tatcgcccac accgatcgct aatgcaccgc ccgagccacc ttcgccaatt
     1501 accgtacaga ttactggcac ggaaagttga gacatttcac gcaggttacg ggcaatcgct
     1561 tctgactgac cacgctcttc cgcaccaatg cccggataag ctcccggtgt gtcgataaag
     1621 gtgatgattg gcatattaaa gcgttctgcc atttccatta aacgtaacgc tttgcgatag
     1681 ccttcaggag caggcatacc aaagttacgt ttcactttct cttttacggt acgacctttc
     1741 tgatgaccga taaccatcac cggacgtccg tccagacggg caataccacc gacaatcgct
     1801 ttatcatcgg caaaggcacg gtcgcctgcc agttcgtcaa attcggtgaa aatatgctca
     1861 atataatcta aagtgtacgg gcgattgggg tggcgtgcca tacgggaaat ttgccacgca
     1921 tctaaattag caaaggtttt cttggttaat tcctcgcttt tcttttgtaa acgcttgatt
     1981 tcatcatcaa gattaatttt gtcatcttgc tcgctgactg ctcttaaaga ctcgatcttg
     2041 gcttccaatt cagcaatcgg caattcaaaa tctaaaaatt cttggctcat tctattttcc
     2101 tttttatggg caaaattatg gggcattatc gcttatcagt cggttattgt ccattttcaa
     2161 ttacgctaaa ttgacccatc gtacctctat cacgcagcat aaaatcgctc acgccaaagg
     2221 taaatggcag ttgctcaggg gcagtattat caaagcgaac caacagcgtg atttcttgct
     2281 ccgcctcgag ccaaacggtg tcccgccacg caagttgttt gatagggtat tgcttgcggt
     2341 ttagggtttc aataatgaat tttgcacctt gtagcgtaaa tccgaccgct tgtgtgctat
     2401 gtaaatacca acgctcaacg ctgccttttt gcgcggtaaa atcaatgcgt tttgggtcaa
     2461 accgcttttg gttgattaaa taatcagccg gacgaatggc aaaacgtctc tcttggctga
     2521 ttttcaggtt aaattcctct aagttgaaag ccggtaaact cggtttatta ttcaataccg
     2581 ctgccattcc ttcagggcga agctctaaaa tcacgttatc attcaattcg tcgttgtcag
     2641 caaagacctg cccgattttg taaaaaatat ctcttttttg accgcttatc agcgataccg
     2701 ctttcccttc gcttaaatcg accaacactt ccaccctttc actcggtgca agttgaaccg
     2761 attcggtttc aagtggctct gccagcattc ctaagccggt ggcgattagg tacaacggct
     2821 gctcgttatc taagcgtaat tcataagcac gggaaagtga ggcattgacg atccgcaatc
     2881 tgacccagcc acgaggcacg ttaaaataag gcgattcctg cccgttgaca aataatcgct
     2941 taccgaaaaa ttgtgaggtg tttttatcta acacctgtac gccgtctttg ttgagcagct
     3001 ggtcttgcag aattaacgga atgtcgttca cgccatattt attcggtaaa gtggcttttt
     3061 tgctctgttc atcttcaatc atccacaagc cggctaagcc acgatacact tggaatgccg
     3121 agttgagcat tgtatcggcg tgataccaac aagtacaagc cggttgattg atagaaacaa
     3181 tcggcgacca gctacttttg gggtcaaggg aacgatgcac cgtgccaatc atctcacttg
     3241 aggcttgcaa tccttgaata ttcattgaga ttttttgcgg taaattattg agataagtta
     3301 atttgacaaa atcccccgat tttacccgta cggttggggc aagatattgc ccgttcacac
     3361 cccatacatc aaccaatttg cctttatcaa attgggtttg tgcggggcgt aaatccaacc
     3421 gaaccggctt tcctcgccca atatccatta aaggcggaat gttcagttgc ggtcgctctg
     3481 ctgctaatag cggtgttggc actgttgcaa atccagccgc aagggcagag ccttgtaaaa
     3541 attggcgtcg ggtgatctgt tttttcatat taggcgtttt ttgcagtgtt ttctaactgt
     3601 tcgacttctt gattaagttg ttcaaatttt gccaacataa tttgatggca cttttccatc
     3661 agttctttga cgttttcacg ggaatagcct gaagtatcaa tcggatcaag catttcacag
     3721 attactgtgc cgttatttga acggttcaaa tccactttat tgtgtaaatt agagcaaact
     3781 accggcacaa tcggcacgcc ggcagaaatg gctaaatgga atgcaccggt tttaaatggc
     3841 agtaaaccac gcccacggct acgagtacct tccgggaaca tccacaccga cacattgcgt
     3901 tctttcacga tttgccctgc tttattcatt gtttcaatcg cactggaacg cttttcacgg
     3961 tgaataaaaa tattgccggt tgcccagtaa accaaaccga aaaacggaat ccaaatcagg
     4021 ctttttttgc cgatactcac cgtcttttct gccaccattg aggaaatcgt taccatatcg
     4081 taattgtttt gatgattgcc gatatagaca accggatagt taatttccgg ctttttgcgg
     4141 gtaattaatt ccaacccgac taatttatgc aaagcaccaa accaacgagc taccacaccc
     4201 acgctacttg ggtgacgtaa acgcagtaac gcataaatag tgccaaaaat agaaatgaaa
     4261 atagcggcaa gggcaacaat taaaatacga aaaattttta acataatgga ataaccttaa
     4321 aaataaattg tctaggagta tagcaaaata aggttatttt ctaaaggcta tccacattaa
     4381 attgatagtt tcctattttg caaaaaatcg gtatatttaa accgcttgtt tatttgataa
     4441 ggagaaaaaa atgagtaaag tgactttagc aggaagcccg attgatgttg caggcacatt
     4501 tccacaaaaa ggcgatgtgg ttgccgattt taccttagtc aacagtgatt taagtgatgt
     4561 ttcgctttcc gattttgatg gcaaacgcaa agtgttaaat attttcccaa gtattgacac
     4621 cggcatttgt gccacttctg tgcgtgtgtt taatcaaaaa gcggctgatg tagcgaatac
     4681 ggttgtcctt tgtatttcag cggatttacc ttttgcccaa gcacgtttct gtggagcaga
     4741 aggaattgaa aatgccaaag tattatccag tttccgtcat cgtgatttac acaacaaact
     4801 gggggtagat attatttcag gtccgttggc aggcttaact tcacgttcag taattgtgtt
     4861 agatgaaaac aacaaggtat tacacagcga attagtgccg gaaattaaac aagagccgga
     4921 ttatgaggcg gcgttagcgg tattataatt tgcagaatta tgctggaaaa taaccgcttg
     4981 taaagcaaca aaaagcccgt tgattcaatt caacgggctt tgtttttagt gattatttct
     5041 tacgagagtg taagcctttt ttctcaaggt tacggtaaat caaccattgc tgtgcaatcg
     5101 tgattaagtt tgaggttaac cagtaaagca ctaagcctga tgggaaccat aagaagaaca
     5161 cggtaaagat aaccggcata aaggtcatta ctttttgttg taccgggtca gctaccggtg
     5221 ttggcgacat tttttgtaac aagaacatcg aaccacccat taataaaggc aaaatgtagt
     5281 atgggtcttg tgccgataag tcttgaatcc agccgaagaa aggtgcgtgg cgtaattcta
     5341 ccgcttccat aaatgtccaa tataaagcaa tgaaaatcgg catttggatt aaaattggta
     5401 agcagccacc cattgggttc actttttctt ctctgtagag tttcatcatc tcttggctca
     5461 tacgttggcg gtcgtcgccg aaacgttcac gcatttcagt aatgcgaggt tgtaacatac
     5521 gcatttttgc catcgaggtg tattgtgctt tggttaatgg gtaaagaatg gttttcacta
     5581 caatagtgac accgataatt gccaagcccc agttgcttac gattttttga attgcggtta
     5641 gtaaccagaa aagcggttta gcgataaacc acgcccagcc gtaatctacg gttaagtcta
     5701 ggtgatttgc tgattcgccc atttctttct gatctttaga acctgtccaa agtttgctat
     5761 ttaaattagc ctctgaattt ggcgcaatcg tagttaaagg gccacggtaa ccgatagttg
     5821 ctaccccatt attggtgcgt gaataaaggc tgttatccgc atcttggttt ggcacccaag
     5881 cagacacgaa atagtgctgt aataccgcaa tccaacccgc tttggtgtcc acgcttaagt
     5941 tggctttttc catatcttgg aaactgtatt ttttgtagtt ggtttctgaa gaagaataag
     6001 ccccgccggt gtaagtcggc atggttaaat tgccggaact ttcaactata gagtgtttta
     6061 attgcccgta aggctgaact tcaatcgctt gtgcagtatt atttttgata ttgaagttta
     6121 ccccaacatc gtagctacca cgttttagcg tgaaagtttt ggtgtaaacc acaccctctt
     6181 tttcaaaggt taatggtaca gaaatttcat tttgcccttc ggctaatttg aagctatctg
     6241 ccgttaccgc atattgtggg cgaccggcat tggtatcaat cccgttttta cccactaaac
     6301 cgctttgagc aacgtagatt ttgtcgccgt cttgctgtaa tagtttaaat ggcgtgttag
     6361 agtgtaattc agcattgtgt tttaacaaat ctgagccgat gacatcaccg cctaaggtat
     6421 caatagtcag acgtaataca tcgctttcca cggtaatggt tttaccttgc gtgatgctat
     6481 ctgcgattgg attattggca ttggaagcag acggaagatc acccgcttgt tgggtttgtg
     6541 ctgccacttg agcttgtttt tgtgcttgaa tttcaggatc aaaatcttgt tgccattgtg
     6601 taaaaatcaa aatagacaca agaaataaac ccattagcag taagccacga ttattcatgt
     6661 tggaattctc ttgttagtaa tggataaaaa gaaaggtatt ttataatagg ttagcttgtt
     6721 ttccaaacgc cttttatgaa caagcggtcg gattttccaa gttttttgca aaattttcgg
     6781 agaatttgac cgcttgtgga gaataagaaa ctagcgttta tctaaggttt tggtggaaag
     6841 gcgagcaact gcacacaggt taccttgttc gtctttaatg tcgatattcc aaacctgtac
     6901 ttcacgccct aatttcagcg gtgtagcttt ggcaatcgct tttgtgcctg ccggaatcga
     6961 ttttaaatgg ctgatgttta gctccattcc caccgcaatt tgatggggtt cacacaccat
     7021 taacgcccct gcattggcag tagtttccgc gagtgctgcc gatacaccgc cgtgcaatac
     7081 gccaaacggc tgttgagtgc gttcgtccac ctgaagctgt gcttcaatcc aatcttcacc
     7141 gatagcggta aattcaatcc ctaagtgtga caccgccgat ttttggctga gtgtgtttaa
     7201 ttgttcacaa gttgctgttt gcttccaaat ggtcattatt atttcctgtc taataaaaaa
     7261 gctgtctatt tagacagctt tcattatctc tttgagcgaa acatactacg ccaatagtac
     7321 attgtttggt cgcccatatt accttaagcc atatagcact aaggaggtga tttatgtgag
     7381 aaaatcagaa gttttctttc tggttgcgtt gctaatatta ttagcaactt ccgctagcat
     7441 taaagtactc accatacttg atgtgctagt ttggttattc gctagataac ctaaaatcag
     7501 acagaggcag gctggttacc tgcctcgctt tttattctaa gtacttttag gttacatttc
     7561 aaccttaaat cacgtccagc aacgcttgaa ttgggtgttt aatgccttgt tgttcaaaac
     7621 gtttgacttg cgagcggcag gagtagccgg ttgctaggca acgctcaagc ggtttatttt
     7681 ttaatttaat ttgccatgat tgctgataaa tcgctttcga catttcaacg tgtttggttt
     7741 cgtgtccgaa cgtgcctgcc attccacagc aaccgacatt ttcattgagt aaggtttcac
     7801 caaaatgggt gaaaatagct tgccactctt tcattgagct tggcaaggcg gtggattcgg
     7861 tgcagtgagc aaataaatac cacggcaagc ggtcggaatt tgccgatttt ttgcaatttt
     7921 ccagctcgcc gtttttaatc acttccctta accattcgtg tgccaataag actttgaaat
     7981 ccccccgagc ttctttgagg atctctttgt attcttcacg ataaagcaaa gttaaggcag
     8041 gatcgacagc caccatcggt agccccaatt tcgccacacg gtttaaaaaa tcggcttggt
     8101 ttttcgctgt tttcgcaaat ttatgcaaaa agcctttaac gtgttgtgct ttaccgcttg
     8161 gcttgaatgg caacacaatc ggcttaaagc ctaatttttt aatgagagcg ataaagtctg
     8221 ccaccacttt ggcatcgtaa taagaggtaa acggatcttg cacaataaac aaggttttgc
     8281 aattttttgc agaaaaatca ccgcttgtag cttgttgttc aaggctttct aagctctcgc
     8341 cttggtagcc gacttccacc aactgatgtt gcagatttgg cactgacaat gccggcaaat
     8401 aggtcattcc aatggttttg tttgcagctt tttcggcaaa agttgaggtg ctgaaaaagt
     8461 taaaaaaacg tggttgtttc gccattaaag gggcgataaa ttctaagttt gagaccacat
     8521 aatctttcag tggtcgcaaa tagcggctgt ggtaaagctc gttaaattgc gatctgaacg
     8581 tcggtacgtc aattttgatt gggcattggc tggcacaggc tttgcaggca aggcaggtgt
     8641 ccatcgccgc tttgacttcg tgtgaaaaat catactcttt gtttttattg agcgtgttgc
     8701 gaatttttgc aacaaaatca gctaatttga ccgcttgttt gcgaggtttg aagatcaaat
     8761 cgtccggcga aacgttttgt tcggtaagta atctgagcca ttcacgcaca agagtagccc
     8821 gccctttcgg tgagtaaaga cggttgcccg agactttcat tgaagggcac atcgtgctgt
     8881 gtacatcaaa gttaaaacat agcccgttgc cgttacaatt cattgctcct ttaaattctt
     8941 ctcgcacttg gattggaatt tggcggtcaa aatcggctcg catttgtgag tcaatcgggt
     9001 agagcgaggt ttcgctttct aatggtgtac aaattttacc tgggttcaag cgattgtacg
     9061 ggtcaaataa ggttttgatg taacggagtt cttgccacaa agtttcgcca aagaaacgtt
     9121 cgccgtagta agaacgcata cctttgccgt gttcgcccca aatcaagcca ccgtatttgg
     9181 cggtgagttc gactacttgg tcggaaattt gcttaaaggt ttccacttgg gctttgtcgc
     9241 ataagtcaag agcagggcga acgtgtagca cgccggcatc aacgtggccg aacattccgt
     9301 aaggcagatt atagcgatct aataaagctc gaaattccac gatgtaatcg gcaaggtttt
     9361 ccggtggcac agcggtgtct tctacaaagg cgatcggttt tgcccagcct ttggcattgc
     9421 ctagcaggcc tacggctttt ttccgcattg cgtagatttt ctcgatagaa tttacatcac
     9481 tcgtgagttg gtagccgata atcccggctt cattatttgc cattttttga tcaaggcttt
     9541 ggcagagtgc tttcacttgg gtttcaattt gagtctgatc gcttgaggca tattcaacaa
     9601 tgttaatccc taaaatcggg ttggctgggt cttcggttaa taaatctgac accgaatgcc
     9661 agacgatatc ttgcttggca agattgagca ctttggaatc aaccgtttcc accgataagg
     9721 cttttgcctc taacataaaa ggagcggagc gtaaggcggc atcaaaggaa ttatatttaa
     9781 tattaattaa ggtgcgatat ttgggaatcg gcagtaaatt cagtttggct tcgcaaataa
     9841 aggcaagcga accttcagaa ccggttaaaa tacgggtaag gttaaattcg ctttcaccat
     9901 ggttaaacac attttttaaa tcatagccgg taataaaacg gttaagctga ggcagttcgt
     9961 tcagcacttt ggggcgtaat gttttgcaac ggttgaaaat ttcacggtgc aaggttttgc
    10021 cgagagcgga gagatttaac ccttcaatat gtgcaaaaaa atcggcagat ttgaccgctt
    10081 gtgtctctaa aatatcgccg ttaataagca cagatttgat ggcaagaata tgatcagatg
    10141 ttttgccata ttgcaaagaa ccttgcccag aggcatcggt gttgatcatt ccgcctaaag
    10201 tcgctcggtt actggtagaa agttcggggg aaaagaaaag tccatgtggt tttaaaaatt
    10261 gattgagctg gtctttcacc acgccagcct gcactcgaac ccaacgctct tccacattca
    10321 gttccaaaat ctgattcata tggcgggaga gatcaacaat aatattattg ttaagagatt
    10381 gcccgttagt gcctgtgcca ccgcctcgtg gagtaaaagt taaatgttga tattctggct
    10441 tttgggcaag tttggttaga attacgacat cagaaacgga tttcggaaac agaatagctt
    10501 gcggaatttg ctgatacacg ctgttgtctg ttgccagtga aagcctatct gcataatttg
    10561 atgcaatttc cccactaaaa tgctgattga cgagttcagt taaataggat tgggtggctt
    10621 tatccagttg cggtacttca gagagtctag gtatcataag ctgtgtttgg ttttttat
//
LOCUS       DANLXF010000074        10510 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.074, whole genome shotgun sequence.
ACCESSION   DANLXF010000074 DANLXF010000000
VERSION     DANLXF010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10510
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.074"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            27..2594
                     /gene="clpB"
                     /locus_tag="PXD66_001228"
     CDS             27..2594
                     /gene="clpB"
                     /locus_tag="PXD66_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDL6319536.1"
                     /translation="MNFEKMTTKLQEALAESQSLAVGKDNPYIEPAHLLYALLKQEGG
                     SIASLFTTLNVDVPTLIRELQQILDRLPKVQGGNTQVSQQLVRLLNQSDKLAQQFGDS
                     FISSELFVLAALDDNGDLGKLFKQFGLNKEKLTQAISQIRGGDTVNNQNAEDTRQALK
                     KYTIDLTERAKAGKLDPVIGRDEEIRRAVQVLQRRTKNNPVLIGEPGVGKTAIVEGLA
                     QRIVNGEVPEGLKNKRVLSLDMGALIAGAKYRGEFEERLKAVLNDLAKEEGQVILFID
                     EIHTMVGAGKTDGAMDAGNLLKPSLARGELHCVGATTLDEYRQYIEKDAALERRFQKV
                     LVDEPTVEDTIAILRGLKERYEIHHHVQITDPAIVAAATLSHRYVSDRQLPDKAIDLI
                     DEAASSLRMEIDSKPEPLDKLERRIIQLKLERQALQKEEDEASRQRLAKLDEEMAAKE
                     REYSELEEIWKAEKSALLGTQHIKSELENARIAMDQARRENDFEKMSELQYGVIPNLE
                     KQLQAVENGEKNADAQLLRTKVTEEEIAEVLSKATGIPVAKMMEGEKEKLLRMEEVLH
                     ARVIGQNEAVDAVANAIRRSRAGLSDPNKPIGSFLFLGPTGVGKTELSKTLANFLFDD
                     PDAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRHPYSVVLLDEVEKAH
                     PDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSHLIRENAYKGYDVVKEMVM
                     EVVGQHFRPEFINRIDETVMFHSLGQEHIRSIAKIQLQRLIQRLAERGYEVTVTDAAL
                     DHIGKAGFDPLFGARPLKRAIQQELENPLAQQILSGKLLPNKAVTVDYQNGQIVATQ"
     gene            2808..3950
                     /locus_tag="PXD66_001229"
     CDS             2808..3950
                     /locus_tag="PXD66_001229"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDL6319537.1"
                     /translation="MSQLFPEATIRTQAPYRNDIVGSFLRTDALKKAREDFNQQKITR
                     DELREVTRQEVKKLVELQKQHGVKAVSDGEFSRTWWHLDFLAELDGMDWQETEVFNVN
                     FTGHKPKGQTVKIVGDIDFSESHPFVDAYRLLKEAAGDYPTKFTIPAPTMLHIICCVR
                     AEDYQPLPQYQDEQKLYDDIANAYIKAMHKFYDMGLRNLQLDDTSWGQFCAEDKRKEF
                     AARGFNLTDLAEKYVALLNRIVDAKPADMSITMHICRGNFRSSWFSEGGYGPVAEVLF
                     GKCRVDGFFLEYDTERAGTFEPLRHIKDQQVVLGLITSKFGDLEDKNVIIERIKEAAQ
                     IVPINQLCLSPQCGFASTEEGNILTEAAQWAKLNLIKEIAEEVWGS"
     gene            complement(4007..5398)
                     /locus_tag="PXD66_001230"
     CDS             complement(4007..5398)
                     /locus_tag="PXD66_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HDL6319538.1"
                     /translation="MENTPLIEPSKNTRNSLIFVADAILFIILLNTLPFTPEANKGLA
                     LLIFIAVLWLTEALHVTVTALLIPILAVALGLVKSKEALVAFADPTIFLFFGGFALAT
                     ALHIQQLDRMIANKIMALAKGKLSIAVIYLFAVTAFLSMWMSNTATAAMMLPLAMGIL
                     SKLDQKQNHNTYLFVLLGIAYSASIGGMGTLVGSPPNAIVASQLNLTFADWLKYGLPI
                     MLILFPLMIATLYIVFKPNFSVSFDRSFEKIELNRSRIITLAIFVFIALGWIFGDKIN
                     PIISAFLGINGKIASFDTILALIAAALICITRVANWQQIQENTEWGVLFLFGGGLTLS
                     AVLGQTGASKIMADGVVSLIEGGHFYLIGLIVAAFIIFLTEVTSNTASAALLVPIFIS
                     IAQALNMPPVGLALIIGLGASCAFMLPVATPPNAIVFGTGQIKQSEMIKVGFWLNIIS
                     IFVIATVGYLFWL"
     gene            complement(5558..6457)
                     /gene="cdd"
                     /locus_tag="PXD66_001231"
     CDS             complement(5558..6457)
                     /gene="cdd"
                     /locus_tag="PXD66_001231"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDL6319539.1"
                     /translation="MFRTDITERFEQLKAAKNCEVTQAIIEQLIKQDFHGQLSYEVVD
                     ELCEKFKRSRVELALYCIAIAACYAVTPVSDFNVGAVAIGKNGDFYFGANQEFSGECM
                     QQSVHAEQSAISHAFLAGETLITDVVVNYTPCGHCRQFMNELNSAASLKVHLPHSQNN
                     LLHSYLPDSFGPKDLGIEKVLFDEQPQQFEVKGDHLQQAAIFAANNAYAPYSNAFSGV
                     ALQVGERIVCGKYTENAAFNPTFLPLQSALNYRLFLGLADIPVTRVVLAEAKGSLSSY
                     AMTQALAKSLFGLEIEYLSLQAV"
     gene            complement(6471..7373)
                     /locus_tag="PXD66_001232"
     CDS             complement(6471..7373)
                     /locus_tag="PXD66_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL6319540.1"
                     /translation="MISSSLKLGYFSLIVATLFWGGNFVLGKVLSGAVQPITLTYLRW
                     LPAFLILVLLFFRPTWRVMHLLKPRLGVMWVLGLLGVILFPATLYEGLKTTTALNASL
                     YLAISPVLVLFLNRLVFKEKISPIILSGAIISLLGVLWLLMQGKLTRLFSLAINQGDL
                     WAIASTISWAVYCCVIRLRPQGISNTVFLTVLVGLAVLTLTPFFIYEYVAESAKNLAN
                     LTALQWSGIGYLVIGPSILSYAFWNFGIAIVGSAKGAVMTNFTPLFAAMFSIILLNES
                     VHTYHIISAILITAGILICSYKKH"
     gene            complement(7370..7726)
                     /gene="folB"
                     /locus_tag="PXD66_001233"
     CDS             complement(7370..7726)
                     /gene="folB"
                     /locus_tag="PXD66_001233"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HDL6319541.1"
                     /translation="MSDKVFIRELTAFASIGAYNWEHTIKQRLVFNIDMAWDFSKAAE
                     TDDVQYCLNYAEVSQKVLDFVESQPFKLVETVAYQLAELLQKEYQLSWIRIELHKPKA
                     VAQASSVGVIVERGHQ"
     gene            7796..8401
                     /gene="plsY"
                     /locus_tag="PXD66_001234"
     CDS             7796..8401
                     /gene="plsY"
                     /locus_tag="PXD66_001234"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDL6319542.1"
                     /translation="MNLIPYLLILTAYLLGSISSAVIFCKLAGLPDPREVGSHNAGAT
                     NVLRIGGKSAALGVLLFDILKGTLPVAMGIYFELSPSQLGFIALAVCLGHIFPIFFQF
                     RGGKGVATAFGAISALSFIVAGGAIGTWLLVFLISGYSSLSAVFTALVVPFYIWWFEP
                     QFTFPVALVCCLLVYRHHDNIQRLWRGQEDKLWDKFKKNRA"
     gene            complement(8475..10382)
                     /gene="parE"
                     /locus_tag="PXD66_001235"
     CDS             complement(8475..10382)
                     /gene="parE"
                     /locus_tag="PXD66_001235"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDL6319543.1"
                     /translation="MSEKRYGADEITVLKDLEPVQLRPGMYTDTTRPNHLGQEVIDNS
                     VDEALSGFADKIDVILHADNSLEVVDNGRGMPVDIHSTEKISGVELILTKLHAGGKFS
                     NKNYTFSGGLHGVGISVVNALSERVEIQIKRGGEVYSIAFANGVKVEDLTVIGTCPKK
                     QTGTTVRFYPNPKYFDSPRFSVSRLRHLLRAKAVLCPKLTINFIDKINNTEESWYYED
                     GLSDYLSEALNGYETVPNPPFIGDVTAETEAVSWALTWLPEGGELVAESYVNLIPTAQ
                     GGTHVNGLRNGLLKAMVEFCEIHNLLPKGVKLTADDVWNRCAYVLSLKIQEPQFAGQT
                     KERLSSRQASSYVDSTLKDAFSLWLNQNVQTGKLIAEMAISSAQSRMRAAKKVVRKKL
                     VSGPALPGKLADCTAQDLSRTELFLVEGDSAGGSAKQARDKEYQAILPLRGKILNTWE
                     VSSDQVLASQEVHDIAVALGIDPDSDNLEELRYGKVCILADADSDGLHIATLLCALFL
                     RHFPKLVQNGHVYVAMPPLYRIDIGKDEVHYALDEAEKEAILARLAKKKGKPNVQRFK
                     GLGEMNPSQLRETTMEPSTRRLVQLTFDDTVEEGELNTFEIMDMLLGKKRAEDRKQWL
                     QNRGDEADLAV"
BASE COUNT         3262 a         2314 c         2108 g         2826 t
ORIGIN      
        1 tctaaactca ttgtaaggag aaagaaatga attttgaaaa aatgaccaca aaactgcaag
       61 aagccttggc tgaatcacag tcgcttgcgg taggaaaaga taatccgtac atcgaaccgg
      121 cacatttgct ctatgcgttg ttaaaacaag aaggtggctc gattgcttct ttgtttacca
      181 cattaaatgt tgatgtaccg actttaattc gtgaattaca acaaatttta gaccgcttgc
      241 cgaaagttca aggcggtaac acccaagttt cacaacagtt ggtcagactg ttaaatcaat
      301 cagacaaact ggcacaacaa ttcggcgaca gctttatttc atcagaacta tttgttctag
      361 ctgcacttga tgacaatggc gatttgggga aactatttaa acaatttggc ttaaacaagg
      421 aaaaactaac acaagccatc agccaaattc gaggaggaga cacagtgaat aatcaaaatg
      481 cggaagatac ccgccaagca ctcaaaaaat atacgattga tttaaccgaa agagccaaag
      541 ccggtaaatt agacccggtt atcgggcgtg atgaagaaat tcgccgtgcg gtgcaagtct
      601 tacaacgccg taccaaaaac aaccctgtgt taattggtga accgggtgtg gggaaaacgg
      661 cgattgtaga aggcttggca cagcgtattg taaacggcga agtgccggaa ggcttgaaaa
      721 ataagcgtgt actttcgctg gatatgggag cattaattgc cggtgcgaaa tatcgtggtg
      781 aatttgaaga gcgtttaaaa gcggttttaa acgacctggc gaaagaagaa gggcaagtta
      841 tcctctttat tgatgaaatt cacacgatgg ttggagcagg caaaacagac ggagcaatgg
      901 acgccggcaa cttattaaaa ccaagccttg cccgtggtga attgcactgt gtgggggcta
      961 caacccttga tgaatatcgc caatacattg aaaaagacgc agcccttgaa cgccgtttcc
     1021 aaaaagtatt ggtggacgaa ccaacggttg aagatacgat tgcgatttta cgtggtttga
     1081 aagagcgtta tgaaatccat catcacgttc aaattacaga ccctgccatc gtggcggcgg
     1141 caacgctttc gcaccgttat gtgtctgacc gtcaattgcc ggataaagcg attgacttga
     1201 ttgatgaagc cgcttcaagc ctgcgtatgg agattgattc taaacctgag ccgttggata
     1261 aattagaacg ccgcattatc caactcaaat tggaacgcca agcgttacaa aaagaggaag
     1321 atgaagcgag ccgtcagcgt ttagccaaat tagacgaaga aatggcagcg aaagagcgtg
     1381 aatattccga gctggaagaa atctggaaag cggaaaaatc cgccttactt ggtacacagc
     1441 atattaaatc ggagttggaa aatgcccgaa ttgcgatgga tcaggctcgc cgtgaaaacg
     1501 atttcgagaa gatgtcggaa ttgcaatatg gcgtgattcc aaacttggaa aaacagttac
     1561 aagcggtcga aaatggcgaa aaaaatgcag atgctcagct cctccgtact aaagtcaccg
     1621 aagaggaaat tgcggaagtg ctttcaaaag caacgggcat tcctgttgca aaaatgatgg
     1681 aaggcgagaa ggaaaaactc cttcgtatgg aagaagtatt acacgctcgg gttatcgggc
     1741 aaaatgaagc cgttgatgcg gtggcaaatg cgattcgccg tagccgtgcc ggactttccg
     1801 atccgaataa accgattggt tcattcctat tcttaggacc gaccggcgtg ggtaaaaccg
     1861 agttaagcaa aaccttagct aatttcttgt ttgacgatcc tgatgctatg gtgcgaattg
     1921 atatgtctga atttatggaa aaacacagcg tatcccgctt agtgggcgca cctccgggct
     1981 atgtcggcta cgaagaaggc ggctatttga ccgaagcggt tcgccgccat ccatattcgg
     2041 tagtgttact tgatgaagtg gaaaaagctc accctgatgt gtttaacatt ttattgcaag
     2101 tgttagatga cgggcgttta accgacgggc aaggcagaac agtcgatttc cgcaatacgg
     2161 tagtgattat gacctcaaac ttaggctcgc atttaattcg agaaaatgcg tataaaggct
     2221 atgatgtggt taaagaaatg gtgatggaag tcgtcggaca gcacttccgc cctgaattta
     2281 tcaaccgtat tgatgaaacg gtgatgttcc actcacttgg tcaagagcat atccgttcga
     2341 ttgcgaagat tcagctacaa cgcttaatcc aacgcttggc agaacgaggc tatgaggtga
     2401 cagtaaccga tgcagcatta gatcatatcg gcaaggctgg tttcgatccg ctgttcggag
     2461 caagaccact gaaacgtgcg attcaacaag agcttgaaaa tccgcttgct caacagatct
     2521 taagcggtaa attattaccg aataaagccg ttacggtgga ttaccaaaac gggcagattg
     2581 ttgcaacaca ataatgtctc cgatggtgcg agcgtcctcg ctcgtacctt acaagcggtg
     2641 gatttatcgt aaaaatttgc aaaaaattgc tcgaaaataa ccgcttgcac agtaaaaatt
     2701 ttcatattgc ccttcattaa ttttcataag ctaatgaagg gctttttatt actctttttg
     2761 ctatcattaa cggcgtttat aaccaataca attacaagga gtaatttatg agccaattat
     2821 ttccggaagc aacaatccgc actcaagccc cgtatcgtaa tgatattgta ggcagctttc
     2881 tacgtaccga tgcgttaaaa aaagccagag aagatttcaa tcagcaaaaa atcacccgtg
     2941 acgaattgcg tgaagtcaca cgccaagagg tcaaaaaatt agttgagttg caaaagcaac
     3001 acggtgtaaa agcagtctct gacggtgaat ttagccggac ttggtggcat ttagatttct
     3061 tagctgaact tgatggaatg gattggcaag aaaccgaagt ctttaacgtg aactttaccg
     3121 gtcataaacc caaagggcaa acggtaaaaa tcgtgggcga tattgatttc tccgaaagcc
     3181 acccttttgt ggacgcttat cgtcttttaa aagaagctgc cggcgattac ccaaccaaat
     3241 tcacgattcc ggcaccgaca atgttacata ttatctgctg tgtgcgtgcg gaagattacc
     3301 aaccgttacc acaatatcaa gacgaacaaa aactgtacga tgatattgct aatgcttata
     3361 tcaaagcaat gcacaaattc tacgatatgg ggctacgtaa cttacaatta gacgacacca
     3421 gttggggaca attctgtgcc gaagataaac ggaaagagtt tgcagccaga ggatttaatt
     3481 taactgattt agcggaaaaa tatgtcgcat tattaaaccg tattgttgat gcaaaaccgg
     3541 cggatatgtc aattacgatg cacatctgcc gtggtaactt ccgttcttct tggttctcgg
     3601 aaggcggtta tggaccggtt gccgaagtat tattcggtaa atgccgtgtt gacggcttct
     3661 tcttagaata cgataccgaa cgtgcaggca ctttcgagcc actacgccac attaaagatc
     3721 aacaagtggt actaggctta attacctcta aatttggcga tttagaagat aaaaatgtga
     3781 tcattgagcg tattaaagaa gcggctcaaa ttgtgccaat caatcagcta tgcttatcac
     3841 cacaatgcgg ctttgcctcc accgaagaag gcaatatttt aaccgaagcc gcacaatggg
     3901 cgaaactaaa cttaattaaa gaaattgcgg aagaagtttg gggtagctaa tcctctataa
     3961 tacaaatagc cgagtaaata ctcggctatt ttagtttgtt ttaacattac aaccagaaga
     4021 gatacccaac agtcgcaatc acaaagatag agataatatt tagccagaaa ccaactttaa
     4081 tcatctcact ttgtttaatt tgccctgtac caaatacaat cgcattcggt ggggtggcaa
     4141 ccggcaacat aaaggcgcaa gatgctccta gaccaataat taaggctaat ccgaccggtg
     4201 gcatatttaa tgcttgtgct atagagatga aaatcggcac taacaaagcc gcactggctg
     4261 tgttagaggt aacctcagtt aggaaaataa taaatgctgc cacaattaaa ccaattaggt
     4321 aaaaatgccc cccttcaatt agtgagacga ccccatcagc cataattttg ctcgcaccag
     4381 tttgtcctaa tacagcactt aacgtcaaac caccaccaaa taggaataat accccccact
     4441 cggtgttttc ttgaatctgt tgccaatttg cgacacgagt aatgcaaata agtgctgcgg
     4501 caatcaatgc aagaatggtg tcaaagctcg caattttgcc attaattccc agaaatgcag
     4561 aaataatagg gttaatttta tcaccaaaaa tccaaccaag cgcaataaag acgaaaatag
     4621 ccaaggtgat aatacgagaa cgatttaatt caattttctc aaatgatcga tcaaaactaa
     4681 ctgaaaaatt tggtttaaat acgatatata gtgttgcaat cattaatggg aataaaatta
     4741 gcataattgg caaaccatat tttaaccaat cagcaaaagt cagattcaat tgagaagcaa
     4801 caatcgcatt cggtgggctt cccactaaag tacccattcc accaatactg gcactataag
     4861 caattcctaa taacacgaaa agataagtat tatggttttg tttctgatca agtttactta
     4921 aaatccccat tgccaaaggt aacatcattg cagctgtagc tgtattactc atccacattg
     4981 acaaaaacgc tgttaccgca aataaataaa taactgcaat cgataattta cctttagcta
     5041 atgccataat tttattggca atcatacgat ctagctgctg aatgtgtaat gctgtcgcta
     5101 aggcaaagcc tccaaaaaat aagaaaatcg ttggatcagc aaaagcaaca agagcttctt
     5161 ttgatttaac cagccccagt gctacagcca aaatcggaat taataatgcc gtaaccgtaa
     5221 cgtgtaatgc ttctgttagc cataagacgg caatgaaaat taacaatgcc aaccccttat
     5281 tggcttcagg ggtaaaaggc aaggtattta acaaaataat aaataaaata gcatctgcaa
     5341 caaaaatcaa gctattgcgt gtatttttac tcggttcaat aagaggagta ttttccataa
     5401 aaacctcaca aaatatagat aatcagtggt atcactatat ataaaaaaac gatataggca
     5461 aatataattg ttgcaaaagt gtgaagctca tcaaaaaatt taacactaaa aaataaattg
     5521 cttaaagata cagcaccttt gcaaaaataa atcaaaatta caccgcttgt agcgaaaggt
     5581 attcaatctc taacccaaat agtgattttg ccaatgcttg agtcattgca taactactga
     5641 gagagccttt cgcctcagcc agcacaactc gagtaacggg aatatcggca agccctaaaa
     5701 ataatcgata gttcaacgcg ctttgcaacg gtaaaaaagt aggattaaaa gctgcattct
     5761 cagtatattt accacaaaca atacgctcac ctacttgcag tgcaacccca ctaaatgcgt
     5821 tgctgtaagg ggcataagca ttattggcag caaaaattgc cgcttgttgt aaatgatcgc
     5881 ccttaacctc aaattgttgc ggttgttcat caaataagac tttctcaatc cctaaatctt
     5941 tcggtccaaa actatccggc aaatagctat ggagcaaatt attttggcta tgcggtaaat
     6001 gtacttttaa gcttgccgca ctgtttaatt cattcataaa ttggcggcaa tgcccacaag
     6061 gggtatagtt cacaaccaca tctgttatca aagtttcccc tgctaaaaat gcgtgcgaga
     6121 ttgcactttg ctctgcgtga acggattgtt gcatacattc cccggaaaac tcttggtttg
     6181 ccccaaaata gaaatctccg tttttgccaa tcgcaaccgc ccctacatta aaatcggaaa
     6241 ctggtgtaac cgcataacag gcagcaatgg caatgcaata taaagccaac tcaacacgag
     6301 agcgtttaaa tttttcacag agttcatcaa ccacttcgta agagagctgc ccgtgaaaat
     6361 cttgcttgat aagttgctca ataatagctt gtgtaacttc acagtttttc gctgctttta
     6421 attgttcaaa acgctctgta atgtctgttc gaaacatact gtacctccgt ttaatgtttt
     6481 ttatagctgc aaatcagtat ccctgccgta atcagtatgg cactaataat atggtaagta
     6541 tgaactgatt catttaataa aataatactg aacattgctg caaagagcgg cgtaaaatta
     6601 gtcattaccg ctcctttagc agaaccgact attgcaatgc cgaaattcca aaacgcataa
     6661 gagagaatag aagggccgat aaccaaatag cctatcccac tccattgtaa agcggtcaaa
     6721 tttgctaaat tttttgcaga ttctgctaca tattcataga taaaaaatgg cgttaacgtt
     6781 agaactgcca gccccactaa tacggtcaaa aacaccgtat tgcttatacc ttgtggacgt
     6841 agccgaatca cacaacaata aaccgcccag cttatagtag aagcaatcgc ccataaatca
     6901 ccttgattaa ttgccaagct gaataagcga gttagcttcc cttgcatcaa tagccacagc
     6961 actcccagca aactgattat tgccccactt agaataatcg ggctgatttt ctccttaaaa
     7021 acgagccgat ttaaaaatag caccaataca ggcgaaatag ctaaatagag actcgcattc
     7081 aatgctgttg tcgtttttaa accttcatat aaggtagccg ggaataaaat taccccaagc
     7141 agacctaata cccacatcac acctaaacga ggttttaata agtgcattac ccgccaagtt
     7201 gggcgaaaaa agagcaaaac taaaattaaa aaagccggca accaacgcaa ataggttaag
     7261 gtaataggct gaactgcccc acttaacact ttgcctaata caaaattccc accccaaaaa
     7321 agagtagcaa cgattaaact gaaatacccc aatttcagtg atgatgagat cattgatgac
     7381 ctcgttctac aatcacacct acactgcttg cttgggcaac cgctttcggc ttatgcagtt
     7441 caatccgaat ccaagaaagt tgatactctt tttgtaatag ttccgccagt tgataggcaa
     7501 cagtttcaac taatttgaaa ggttgagatt caacaaaatc cagcactttt tgcgatactt
     7561 ccgcataatt caagcaatat tgtacatcat cggtttctgc cgctttggaa aaatcccacg
     7621 ccatatcaat gttaaaaacc aagcgttgct taatcgtatg ctcccaattg taagccccaa
     7681 tggaagcaaa agcggttagt tctcgaataa aaaccttatc actcattttt cgtcctgttt
     7741 tattttctga atttctgtaa aatatagcac aagataaatt taatcaggaa aatttatgaa
     7801 tttaattccc tatttattga tcttaaccgc ctatttgctc ggctcgattt ccagtgcggt
     7861 cattttttgt aagctggcag gcttgcctga cccaagagaa gtcggctcac acaatgccgg
     7921 tgcaacgaac gtactacgaa ttggcggtaa aagtgccgca ttaggtgtgc ttttattcga
     7981 tattttaaaa ggcacattac cggtggcgat ggggatttat tttgaactct ctccctcaca
     8041 actcggtttc atcgccttag cggtttgtct tgggcatatt ttcccgattt tcttccaatt
     8101 ccgtggcgga aaaggcgttg ccaccgcatt tggtgcaatc tccgcattaa gctttattgt
     8161 tgcaggcggt gctatcggca cttggctact ggtgttttta atctccggct attcttcttt
     8221 aagtgccgtc ttcactgcct tggtcgtacc gttttacatt tggtggttcg agccacaatt
     8281 tactttcccc gtcgccttag tttgttgcct attggtttac cgccatcacg ataatattca
     8341 acgcttatgg cgtgggcagg aagacaaact gtgggataaa ttcaagaaaa atagagcata
     8401 aaaataggca tagtgaaccg ttcgctatgc ctatttgcaa attattgcgg aaaaatcacc
     8461 gcttgtcttc tcgactaaac cgctaaatct gcttcatctc ctcgattttg tagccattgt
     8521 ttacgatctt ccgctcgttt tttacccaat agcatatcca taatttcaaa ggtattcaat
     8581 tcgccctctt ctaccgtgtc atcaaaggtt aattgtacta agcggcgagt gcttggctcc
     8641 atcgtagttt cacgcaactg gctcgggttc atttcgccta agcctttaaa gcgttgcaca
     8701 tttggctttc cttttttctt ggcaaggcga gctaaaatcg cctctttttc cgcctcatct
     8761 aaggcataat gcacctcatc tttgccgata tcaatgcgat aaagtggtgg cattgccaca
     8821 taaacgtgtc cgttttgcac caatttcggg aaatgacgaa ggaataacgc acacagcaag
     8881 gtggcaatat gcaaaccgtc tgaatccgca tcggcaagaa tacaaacttt gccataacgc
     8941 aactcttcta aattatcgct atcagggtca attcccaagg ctaccgcaat atcgtgtact
     9001 tcttgtgaag ccaatacttg gtctgatgaa acctcccaag tgtttaaaat cttaccacgc
     9061 agtggcaaaa tggcttgata ctctttatct cgagcttgtt ttgccgaacc gccggcagaa
     9121 tcaccttcta ccaagaaaag ttcggtacgg ctgagatctt gagctgtaca atccgccagt
     9181 ttgcccggta aagccggacc actcaccaat ttcttacgca ccactttttt cgccgcacgc
     9241 atacggctct gggcggaact aattgccatt tcagcaatta atttacctgt ctgcacattt
     9301 tggttaagcc ataaactaaa ggcatctttc aaggtgctat ccacatagct tgaggcttga
     9361 cgggaagaca aacgctcttt ggtctgccct gcaaattgtg gctcttgaat tttcagagaa
     9421 agcacataag cacaacggtt ccacacatca tcagcggtta attttacccc tttcggcaat
     9481 aaattatgga tttcgcaaaa ctccaccatc gcctttaata aaccgttacg caagccatta
     9541 acgtgcgtgc cgccttgtgc ggtcggaatc aggttcacat agctttccgc tactaactcg
     9601 cccccttccg gcaaccaagt taatgcccag cttaccgcct ctgtttcagc agtaacatca
     9661 ccaataaacg gtggattcgg cacggtttca taaccgttta atgcctcgct caaataatca
     9721 gataagccgt cttcgtaata ccaactttct tcggtattgt tgattttatc aataaaattg
     9781 atggtgagtt tcgggcaaag caccgctttg gctcgtaaca aatggcgtaa acgactcacc
     9841 gaaaaacgag ggctatcaaa atattttggg ttcgggtaaa aacgcactgt cgtgccggtc
     9901 tgttttttcg ggcaagtacc aattaccgtc agatcttcca cttttacccc gttggcaaag
     9961 gcaatcgaat agacttctcc gccacgttta atctgaattt caacccgttc ggacagggca
    10021 tttaccaccg aaatccccac cccgtgcaaa ccaccggaaa acgtatagtt tttattggaa
    10081 aatttaccgc cggcgtgcaa tttcgttaag ataagctcca cccctgaaat tttctcggtg
    10141 gaatgaatat cgaccggcat tccccgcccg ttatccacca cttccaacga attatcagca
    10201 tgaagaatca catcaatttt atctgcaaag ccggacaacg cctcatcgac actattatca
    10261 atcacctcct gccccaaatg gtttggacgt gtggtatccg tgtacattcc cgggcgaagc
    10321 tgcaccggct cgaggtcttt cagaacggta atttcatccg ccccatagcg tttttcagac
    10381 attatgcttt ctcttaggct taaaataaaa taaggctgaa ttttaaggaa tattcagcta
    10441 ttttgcaaaa ttttggtgaa aaatgacctg ttgttatgca ataaaaacta tttttgcgat
    10501 ctacatcgaa
//
LOCUS       DANLXF010000075        10386 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.075, whole genome shotgun sequence.
ACCESSION   DANLXF010000075 DANLXF010000000
VERSION     DANLXF010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10386
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.075"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..251)
                     /locus_tag="PXD66_001236"
     CDS             complement(<1..251)
                     /locus_tag="PXD66_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319544.1"
                     /translation="MGIKVLFGTKISAELQYLPEKDLVKILQFKQHVEIYGLENLAGR
                     NKSSDDVPTNDPYWAEKVAKAQKYSLWHYHIGIPEYDTS"
     gene            complement(241..714)
                     /locus_tag="PXD66_001237"
     CDS             complement(241..714)
                     /locus_tag="PXD66_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319545.1"
                     /translation="MMKNLPLSMADTLAVIYPAFDFAADDKPATEQQISLLNFRITMA
                     NQLLIACIETIEKNPNFWAEIDLNDFKKYLNQNKVVIFDTQQYFTDGSRTMAILDNMK
                     SNIRKIEQQLSQATAFNFDLERMKERLNDTFHEVPKDITNVNEFRNWLKGVANGH"
     gene            complement(782..1870)
                     /gene="rlmM"
                     /locus_tag="PXD66_001238"
     CDS             complement(782..1870)
                     /gene="rlmM"
                     /locus_tag="PXD66_001238"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HDL6319546.1"
                     /translation="MFNKLALYCRAGFEKEVAGEITDKAAQQGIFGFANLKENSGYVI
                     FECYQAGEADRLAQALKFEQLIFVRQMLVVGDLLQNLSENDRISPILAQYAELNPRQS
                     SDVFVETPDTNEAKELLTFCRKFTVPLRSALKKQGWLGKSENMQGSVSLHILFTRPGA
                     CYVGYAYNHNKSPFFMGIPRLKFPAEAPSRSTLKLEEAILTFIPQAEEKKRFNDTMVG
                     VDLGACPGGWTYQLVKRGVFVYAVDHGKMAASLHETGRIEHCPEDGFKFQPPKRKQID
                     WLVCDMVEQPIRISKLIAKWLTNGWCRETIFNLKLPMKKRYQEVQLCLNFLEEELTKH
                     GFWFKMQAKHLYHDREEITVHIAVMGQK"
     gene            complement(1870..2262)
                     /locus_tag="PXD66_001239"
     CDS             complement(1870..2262)
                     /locus_tag="PXD66_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HDL6319547.1"
                     /translation="MKNKFVAIAAFSGLFTVAFGAIASHALSAQLSPKALGWIETGIK
                     YQMFHTLAILALGLFQIANFGQNPPVCRAKAFNIIGSNWALGILLFSGNLYLLALNVP
                     TIHWLTPIGGLCFMLGWIALIYISVRKA"
     gene            complement(2369..3770)
                     /locus_tag="PXD66_001240"
                     /pseudo
     CDS             complement(2369..3770)
                     /locus_tag="PXD66_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
     gene            complement(3925..6282)
                     /locus_tag="PXD66_001241"
     CDS             complement(3925..6282)
                     /locus_tag="PXD66_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HDL6319548.1"
                     /translation="MNTALEKGKFVFLDKPKNTITTAAHWGALNVTVENGKVVKSEGS
                     LAKNHQNELQDVVADQLYSPVRVKYPMVRKGYLEGNKDTTLRGRDKWVRISWDKAFEL
                     VSGEIQRVREQYGSESIFGGSYGWYSSGSLHASRTLLQRYLNVTGGFTNTKGDYSTGA
                     VQVIMPYVLGNIEVYAQQTSWHTIVESTELLVIWSSNPLNTLRIAWTSAEPEGIDYLA
                     KLKASGKRIICIDPVKSETCQFLEAEWLPINTGTDVALILGIAHTLITENKHDHDFLT
                     KYTVGFEQFADYVLGKADNLPKTAEWASQICDIPEEVIKTLANDFVTHRTMLMSGWGL
                     QRLQHGEQSHWALVTLAAMLGQIGLPGGGFGLNYRYSTNGLNVNSGVRLGAINAGKPN
                     PDMPFFPVSRIADALLKPNQPYDYNGQTLHYPNIKLVYWAGGNPFAHHPDTNYLVKAW
                     QQPETMIVNECYWTPTARMADIVLPATTSYERNDLTVAGDYVAKHIIPMKQVVEPQFE
                     AKNDYDIFAELAKRAGKEQAFTEGKSEMEWLKEFYQVAFEAGEKANTPLLDFEAFWAE
                     NKVLSFEAEHQNNTFVRYQDYRTDPIKHKLATPSGKIEIFSETIAKMNYEDCKGHPTW
                     FEHHEYSGKTNEAEPLALVTPHIKYRLHSQLAYSSLRQYYEVNGREPMLIHTKDAADR
                     GITSGDIVRIFNKRGQVLAGAVVTDGIIQGTVALYEGGWYDPQDLGKTDTPLCKNGNP
                     NVLTRNEGSSKLGQGNAPNTTTVQVEKYVGDAPEVTVFKAPFFSE"
     gene            complement(6418..9024)
                     /gene="acnB"
                     /locus_tag="PXD66_001242"
     CDS             complement(6418..9024)
                     /gene="acnB"
                     /locus_tag="PXD66_001242"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014391308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HDL6319549.1"
                     /translation="MENFIENYQQQVDERAALGVVPQPLSAEQTAQLVELLKNPPAEQ
                     AVFLLDLFTNRIPAGVDEAAYVKAAFLADITKGVATSPLISPAHAVKLLGTMQGGYNI
                     ETLLSALDNPELAPIAVEALSKTLLMFDNFHDVKERADQGNPFAQQVLESWANADWFT
                     SRPKLAEKLTVTVFKVTGETNTDDLSPAQDAWSRPDIPLHALAMLKNERDGIFPDENG
                     HTGPIKQLEALKEKGFPLAYVGDVVGTGSSRKSATNSVLWFMGEDIPYIPNKRAGGVV
                     LGGKIAPIFFNTLEDAGSLPIEVDVTNLNMGDVIDIYPYAGKICKHNSDEVLAEFELK
                     TEVLLDEVRAGGRIPLIIGRGLTHKARVALGLPESDVFIKPQSVSESNKGYTLAQKMV
                     GRACGVEGVRPGQYCEPRMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAA
                     YPKPVDVITHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIG
                     ISFPAGSGLVAFAAATGVMPLDMPESVLVRFSGTMQPGITLRDLVHAIPYYAIQQGLL
                     TVEKKGKKNIFSGRILEIEGLEDLKIEQAFELSDASAERSAAACTIKLNKEPIIEYLN
                     SNITLLKWMIAEGYDDKRTLERRIANMQAWLDNPELLEADENAEYAAVIEINLDEIKE
                     PIVCVPNDPDDARLLSQVQGDKIDEVFIGSCMTNIGHFRAAGKLLSQAQGELPTKLWI
                     APPTKMDAGLLTEEGYYSIYGKSGARVEMPGCSLCMGNQARVANNANVVSTSTRNFPN
                     RLGQGANVYLASAELAAVSALLGKLPTPQEYLDYMNNLQKDKDDIYRYMNFDQINSYV
                     KKADNVIVQVAV"
     gene            complement(9044..10291)
                     /gene="icd"
                     /locus_tag="PXD66_001243"
     CDS             complement(9044..10291)
                     /gene="icd"
                     /locus_tag="PXD66_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HDL6319550.1"
                     /translation="MQTQVQIPQGETIQLNQDGSLQVPDNPIIPFIEGDGIGVDVTPA
                     MKAVLDAAVEKAYQGKRKIAWMEIYAGEKANKLYGENTWLPDETLELIRQYHVAIKGP
                     LMTPVGGGIRSLNVAMRQGLDLYNCLRPIRYYDGTPSPVKHPELVDMVIFRENSEDIY
                     AGVEWVAGSPEANKVIEFLQQEMGVKKIRFTEDCGIGIKPVSKQGTQRLVRAALQYVI
                     DNNRKSLTLVHKGNIMKFTEGAFKEWGYQVAEEFGAKLIDKGPWMSLTNPKTGEEIII
                     KDSIADAFLQEILLHPTEYDVIATLNLNGDYISDALAAQVGGIGISPGANIGFEAAIF
                     EATHGTAPKIAGQNKGNPGSLILSGEMMLRHLGWVEAADLVVKAVSKTIANKTVTFDF
                     AEMLEGVTLRSTSEFAQDIIANM"
BASE COUNT         2929 a         2420 c         2034 g         3003 t
ORIGIN      
        1 tgactggtat catattctgg aatgccgata tgataatgcc ataaagaata tttttgtgct
       61 ttagccacct tctctgccca ataaggatca ttcgttggca catcatcaga acttttattt
      121 cgaccagcta gattttccaa tccataaatt tcaacgtgtt gtttaaactg caggattttg
      181 actagatctt tttcaggcaa atattgcaat tcggctgaaa tttttgtacc aaataaaact
      241 ttaatgccca tttgcaacac ctttcaacca gtttctaaat tcattaacat tcgttatatc
      301 tttaggcact tcgtgaaaag tatcatttaa ccgctctttc attcgctcta agtcaaaatt
      361 aaatgcggtt gcttgagaga gttgctgttc aattttgcga atattggact tcatattatc
      421 caatatagcc attgtccttg agccatcagt aaaatactgt tgggtatcga aaatcaccac
      481 cttattttga tttaggtatt ttttgaaatc gtttaaatca atttcagccc aaaagtttgg
      541 atttttttcg atagtttcaa tacaggcaat taataattga tttgccattg taatacgaaa
      601 atttaacaat gagatttgct gctctgttgc aggtttatca tcagctgcga aatcaaatgc
      661 cggataaatc actgcaagcg tgtcagccat agataagggt aaatttttca tcatctctcc
      721 ttacggaaga agttgtcatt atctactgga ttacttaaaa aaacaactta ttcattttat
      781 gctatttttg ccccatcacc gcaatatgta ccgtaatttc ctcacggtcg tggtagaggt
      841 gtttagcttg cattttgaac caaaacccgt gtttcgttag ctcttcttcc aagaaattga
      901 ggcatagttg cacttcttga taacgttttt tcatcggcag ttttaggttg aaaatcgttt
      961 ctcgacacca accgttagtc agccattttg caatgagttt cgagatacga atcggctgct
     1021 ctaccatatc acaaaccagc caatcgattt gtttacgctt aggcggttgg aatttaaacc
     1081 catcttcagg gcaatgctca attcttccgg tttcgtgtaa acttgctgcc attttgccgt
     1141 gatcgactgc atagacaaaa acaccacgtt tgactaattg ataagtccaa ccacccggac
     1201 aagccccaag atcgacccct accatcgtat cattaaagcg ttttttctcc tctgcttgcg
     1261 gaataaaagt taaaatcgcc tcttctaatt taagcgttga gcgactaggt gcctcagccg
     1321 ggaattttaa acggggaatc cccataaaaa acggcgattt attatggttg tacgcatagc
     1381 ccacatagca agctcccgga cgagtaaata aaatatgcaa gctcacacta ccctgcatat
     1441 tttccgattt ccccaaccaa ccttgttttt tcaatgccga ccgtaatggc acagtaaatt
     1501 tacgacagaa agttaataat tctttcgctt cgttggtatc cggtgtttcg acaaatacat
     1561 cagaactttg gcgaggatta agttcggcat attgggctaa aatcggcgaa attcgatcat
     1621 tttcagacag attttgcaac aaatccccaa caaccaacat ttggcgaaca aaaataagct
     1681 gttcaaactt taacgcttgg gctaatcgat ctgcttcccc tgcttgatag cactcaaaaa
     1741 tcacatagcc actattttct ttaaggttgg caaagccaaa aatgccctgt tgtgcagctt
     1801 tatccgtgat ttcgcctgct acctcttttt caaaccctgc tcggcaatat aaggctaatt
     1861 tattgaacat taggcttttc tcacactgat ataaattaac gcaatccaac caagcataaa
     1921 gcagagacca ccaataggcg taagccaatg aatagtcggc acatttaacg ccagtaagta
     1981 caggttgcca ctaaaaagca gaatgcctaa cgcccaattg cttccgatga tgttaaaggc
     2041 tttggctcta caaacgggtg gattttgccc aaaatttgca atttggaata aacctaaagc
     2101 taaaatggca agggtgtgaa acatttgata tttaatcccc gtctcaatcc agcctaaggc
     2161 tttaggcgat aattgagccg ataatgcgtg gctggctatc gccccaaatg ccacagtaaa
     2221 taagccgctg aatgcggcaa ttgctacaaa tttgtttttc ataagttgaa gataggtatg
     2281 atgttagatg gcttactata tcatattatc gaaaccaaca agcggtgaaa ttttgcaaaa
     2341 aaattacaaa aaatcaccgt ttgtatgctt aatggttcaa gataatatcc acatcttccg
     2401 atttatcgtg aaccccaaat ctatctacta aggttttgct ggcctcattt aatccgataa
     2461 tttctacctc aacatcactt tttcgatatt tcaacacaac tttatctaac gctgaaactg
     2521 ccgttacgtc ccaaaagtga gcgtgggtta aatcaatgtg gacttttttc agttctgaac
     2581 gggtaaaatc aaattgtgcg ataaaatcat cagatgaggc aaaaaatact tgcccgataa
     2641 catcataacg caattcctct ttctctatag cacttgtttt aaccgccata taacgtccaa
     2701 ttttattcgc aaagaataat gccgagagca acacgccgga aaatacacca agggctaagt
     2761 tatgtgtata aaccacaatt gcaacagtga ctaacatcac tacattaaag ccgattgggt
     2821 gcgtttttag ctcttttaag gaatgccaat taaaggtgcc gatagagacc ataatcatca
     2881 ctgcaactaa tgcagccatc ggaatcactc tcaaaatatc gctcaaaaag accacaaaaa
     2941 tcagtaataa tattggcaat gccctgccct ttacattcac ggtttttatc gctgtctgtc
     3001 tctgtcattt catcaacaat cgttgccgtc attaaagatt ccaataaccc tactgcggcc
     3061 aagctgaaag aataaggcaa aataatccat aaggtgtcta aattaagcgg aatatcgggt
     3121 aataaaaaca ccggtaaggt atcgggtaat tgccccatat ccccaacggt acgaacatca
     3181 atgccttgcc ataatgtgaa aagtgttaaa gcaataatgg tcactaatgg tgagggaatg
     3241 atttttccaa tcaccggtac ataaggaaaa agataaatga tagcaagccc tgctaccacc
     3301 agtgcataag tatgccaagt tgtattttgt aactcaggta gttgtgccat aaaaattaaa
     3361 atggcaagtg cattaacaaa cccaatcact acggctcgtg ccacaaaccg cataagcgag
     3421 ccgagtttta accaaccggc aataatttgc aacacgcctg ttaataaggt ggccgctagc
     3481 atatattgta aaccgtgatc cctcactaag cctgctaata ccagagccat tgcaccggtt
     3541 gctgcagaaa tcatcgccgg tctgccgccc acaaaactga taattactgc aatacaaaat
     3601 gaggcatata aaccaatttg tggatcaacc cctgcaataa ttgaaaatgc tatggcttct
     3661 ggaattaacg ccaaagccac cactaaacca gagagaacat cacctcgtac atttgaaaac
     3721 cattgttgtt taattaccgt aggcgaaaat tgctgaaata aagttgtcat tattaccctt
     3781 aaataaaaag aaaagccgag caaagtcggc taaaaatgag gcgagagagt agcaaattag
     3841 tgaaaagtcg tcaaataaac aagcggtctt ctttcgctct ttttttgcaa aaaatagtag
     3901 aaaaatgacc gcttggatta attgctattc actaaaaaaa ggggctttaa acaccgtcac
     3961 ttcaggtgca tctcccacat atttttccac ttgaaccgtg gtagtgttcg gtgcattgcc
     4021 ctgcccgagt tttgatgaac cctcattacg ggttagcaca ttcgggttgc cgtttttgca
     4081 tagtggagta tctgttttgc ctaaatcttg cgggtcatac caaccacctt cgtataacgc
     4141 tactgtgcct tgaatgatgc catcagtcac cactgctcct gccaacacct gccctcgctt
     4201 gttaaaaatt cgcacaatat caccgcttgt aatgcctctg tcagcggcat cttttgtgtg
     4261 aattagcatt ggttctctgc cgtttacctc gtaatattga cgtaatgagg agtaagcaag
     4321 ctggctgtgt aaacggtatt taatatgcgg tgtaactaat gccaacggct cggcttcgtt
     4381 ggttttgcct gaatattcgt gatgttcaaa ccaagtcggg tgtcctttac aatcttcata
     4441 attcatcttg gcaatggttt cggagaaaat ctcaattttg cctgatggcg ttgccaattt
     4501 gtgtttaatc ggatcagtac gataatcttg atagcgaacg aaagtattat tttgatgttc
     4561 tgcctcaaag ctcaacactt tattttccgc ccaaaatgcc tcgaaatcca gcaatggcgt
     4621 attagctttt tcccccgcct caaaggcaac ttggtaaaat tccttaagcc attccatttc
     4681 gcttttgcct tcggtaaatg cttgttcttt accggctcgt ttcgctaatt cggcaaaaat
     4741 atcgtaatca ttcttcgcct caaattgcgg ttcaaccacc tgtttcatcg ggataatatg
     4801 ttttgctaca taatcaccgg caacggtgag atcgtttcgc tcgtagctgg tggttgcggg
     4861 caatacaata tctgccattc gagcggtcgg tgtccagtaa cattcattga cgatcatcgt
     4921 ttccggctgt tgccacgctt tcactaaata gttggtatcc gggtggtgag caaacggatt
     4981 accccccgcc caatagacta atttgatgtt cgggtaatgt aatgtttgcc cgttgtaatc
     5041 ataaggctga ttcggtttga gcaacgcatc ggcaattcga gacaccggga aaaacggcat
     5101 atcggggtta ggtttacctg cattgattgc ccctaaacga acaccggaat taacatttag
     5161 cccattggta gaataacgat aatttaagcc gaaaccgccg cccggcaagc caatttgccc
     5221 gagcatagcg gcaagagtca ctaacgccca gtggctttgc tccccgtgtt gcaggcgttg
     5281 caagccccag ccggacatca acattgtgcg gtgagtaaca aaatcatttg ctaaggtttt
     5341 aatcacctcc tccggaatgt cacaaatttg gctcgcccac tctgctgttt taggtaaatt
     5401 atctgctttt cctaacacat aatcagcaaa ttgctcgaaa ccaacagtat atttagttaa
     5461 gaagtcgtga tcgtgcttgt tttcagtaat aagggtgtgg gcgatcccta aaatcagtgc
     5521 aacatccgtg ccggtattaa taggcaacca ctctgcctct aaaaattgac aggtctcgct
     5581 tttcaccggg tcgatacaaa taatccgttt accgcttgct tttaatttgg cgagataatc
     5641 aatcccctcc ggttcggcag aggtccacgc aatgcgaagt gtattcagtg gattagacga
     5701 ccaaattacc aataattctg tgctttctac aattgtgtgc caactggttt gttgagcata
     5761 cacctcaata ttgcctaata cataaggcat aatcacttgc accgcaccgg tggaataatc
     5821 gcctttagta ttggtaaagc cgcctgtgac gtttaaataa cgttgcaaaa gcgtgcgaga
     5881 ggcgtgcagt gaaccactgc tataccagcc gtaagaaccg ccgaaaatgg attcgctgcc
     5941 atattgctct cttacccgtt ggatttcgcc ggaaaccagc tcaaaggctt tgtcccaaga
     6001 gattctcacc cacttatctc gccctcgcaa agtggtgtct ttattccctt ccaagtagcc
     6061 tttccgcacc atcggatatt tcacccgaac cgggctatag agctgatccg ccaccacatc
     6121 ttgcaattcg ttttggtgat ttttcgctaa actgccctct gatttcacca cttttccgtt
     6181 ttccaccgtt acatttaatg caccccagtg ggcggcggtg gtaatggtat ttttcggttt
     6241 atccaaaaag acaaacttcc ctttttctaa tgctgtgttc atttaaatta atgccttttc
     6301 tgttacaaaa ttgagaggaa tttactggat taagccaaaa gttgcaacca caagcggttg
     6361 tttttagcca aaaatttgca aaacaaaacc cacctaaaaa ggtgggcttc aaggaagtta
     6421 aacggcaact tgaacgatta cattgtccgc ttttttcaca taactattga tttggtcaaa
     6481 gttcatatag cggtaaatat cgtccttatc tttctgcaag ttattcatat agtctaggta
     6541 ctcttgcggt gtcggcaatt tacccaataa cgcagaaact gcggctaatt ctgctgaggc
     6601 taaatacaca tttgcccctt gccctaaacg gttcgggaag tttcgggtag aggtagaaac
     6661 cacattagca ttatttgcca ctcgggcttg gttacccata cagagagaac agcccggcat
     6721 ctctaccctt gctccgcttt tgccgtaaat gctgtaatag ccctcttcgg tcagcaagcc
     6781 tgcgtccatt ttagtcggcg gagcaatcca taatttagtc ggcaattcgc cctgtgcttg
     6841 gcttaacagt ttacctgccg cacggaaatg cccgatatta gtcatacaag aaccaataaa
     6901 gacttcatca attttatcgc cctgtacttg agaaagcagg cgagcatcgt cagggtcatt
     6961 cggtacgcac acaatcggct ctttgatttc atctaagttg atctcaatca ctgcggcata
     7021 ctccgcattt tcgtctgcct ctaaaagctc cggattatcc aaccacgctt gcatattggc
     7081 gatacggcgt tctagcgtgc gtttatcgtc atagccttcc gcaatcatcc attttaaaag
     7141 cgtaatatta gaattcagat actcgataat cggctctttg ttgagcttga tggtacaggc
     7201 tgcagcagag cgttcagcag aagcatcaga aagctcgaag gcttgttcga ttttcagatc
     7261 ttccaaccct tcaatttcta aaattcgacc ggagaaaata tttttcttac cttttttctc
     7321 caccgtcagc aagccttgct gaatggcata atacggaata gcgtgaacca gatcccgtaa
     7381 ggtaatgccc ggttgcattg tgccggaaaa acgcaccaag accgattccg gcatatctaa
     7441 cggcatcacc cctgttgccg cagcaaaggc aactaagccc gaaccggcag ggaatgaaat
     7501 cccgattggg aaacgggtgt gggaatcgcc gcctgtaccg acagtatccg gcaataacat
     7561 acggtttagc caagagtgaa tcacgccatc acccgggcga agtgacacac cgccacggtt
     7621 cataataaaa tcaggcaaag tgtggtgggt aattacgtcc accggtttcg ggtaagcggc
     7681 agtatggcag aacgattgca tcacaagatc tgccgagaag cctaaacagg ctaaatcttt
     7741 cagctcatct cttgtcattg gtcctgtggt atcttgtgaa ccgacagacg tcattcgagg
     7801 ctcgcaatat tgccccggtc gaacgccttc tacgccacaa gctcgcccta ccattttttg
     7861 agcaagggtg tagcctttgt tactctctga aaccgactgc ggtttaataa atacatcact
     7921 ttcaggcaag ccaagtgcta ctctggcttt atgggtcaaa ccacgtccga taatcagcgg
     7981 aattcgcccg cctgcacgca cttcatcaag caatacttcg gtttttagct caaattcggc
     8041 taagacttca tcggagttgt gtttgcaaat tttgcctgca taagggtaaa tgtcgattac
     8101 atcgcccata ttcaggttgg ttacatccac ttcgatcggt aaagaaccgg catcttctaa
     8161 tgtgttaaag aaaatcgggg caattttacc gcctaacacc accccaccgg cacgtttgtt
     8221 tggaatgtaa ggaatatctt cccccataaa ccaaagcacg gagttggttg cggatttacg
     8281 agaagagccg gtgccgacta catcaccgac ataggctaac gggaaacctt tttcttttaa
     8341 tgcttccagt tgcttaatcg ggccggtatg accgttttca tcggggaaaa taccgtcacg
     8401 ttcatttttc agcattgcta atgcgtgaag tggaatatca gggcgagacc aagcgtcttg
     8461 agccggggaa agatcatcgg tattggtttc gcctgtgact ttaaataccg ttaccgttaa
     8521 tttttcggca agtttcgggc gagaggtaaa ccaatcggca ttcgcccaag attccaatac
     8581 ttgttgtgca aacggattac cctgatctgc ccgttctttt acatcgtgga aattatcaaa
     8641 catcaataat gttttagata aggcttctac cgcaatcggt gcaagctcag ggttatccaa
     8701 cgcagacaat aaggtttcaa tgttataacc accttgcatt gtgccgagta atttcactgc
     8761 gtgagcaggc gaaatgagcg gcgatgtggc aacccctttg gtaatatcgg ctaaaaaggc
     8821 ggctttaaca taagcggctt catcaacacc tgccggaata cgattggtaa aaagatccaa
     8881 taaaaagacc gcttgttcag ccggtgggtt tttcaataat tcaaccagtt gagccgtttg
     8941 ttccgcagaa agtggctgag gcacaacacc cagtgccgca cgttcatcaa cctgttgttg
     9001 ataattttct ataaaatttt ccatcatcat tccctctcca actttacata ttggcaataa
     9061 tgtcttgtgc aaattctgag gttgagcgta gagttacccc ctccagcatt tccgcaaaat
     9121 caaaggtgac agttttattg gcaatggttt tagacaccgc ttttaccact aaatcagccg
     9181 cttcaaccca acctaaatgg cgtaacatca tttccccact taaaattaat gacccaggat
     9241 tgcctttatt ttgccctgca attttagggg ctgtgccgtg tgtggcttca aaaatagccg
     9301 cttcaaaccc aatattcgct cccggtgaaa taccgattcc gcctacttgt gcggctaaag
     9361 catcagaaat atagtcgccg tttaggttca gtgtagcaat cacatcatat tcggttgggt
     9421 gtaacaagat ttcttgtaaa aaggcatcgg caatgctgtc ttttataatg atttcctcac
     9481 cggttttcgg attagtcaag ctcatccaag ggcctttatc aattaatttt gccccaaatt
     9541 cttccgcaac ttggtagccc cactctttaa atgctccttc ggtaaatttc ataatgttac
     9601 ctttatgcac aagcgtgagc gatttacggt tgttatcaat tacatattgc aatgcagcac
     9661 gcaccaagcg ttgtgtgcct tgcttagaaa ccggcttaat tccgataccg caatcttcgg
     9721 taaagcggat ttttttcacg cccatctctt gttgtaaaaa ttcgatcact ttattggctt
     9781 caggcgaacc ggcaacccat tctacaccgg cataaatatc ttccgagttt tcacggaaaa
     9841 tcaccatatc caccagctca gggtgtttaa ccgggcttgg cgtaccgtca taataacgaa
     9901 ttgggcgtaa gcagttgtat aaatctaatc cttgacgcat cgccacattt aaagaacgaa
     9961 taccgccacc caccggtgtc attaaaggac ctttaatcgc cacgtgatat tggcggataa
    10021 gctctaaggt ttcatccggc aaccaagtgt tctcaccata taatttattc gccttttcgc
    10081 ctgcgtaaat ttccatccaa gcaatttttc gcttgccttg ataggctttt tctaccgccg
    10141 catctagcac tgctttcatt gcaggagtaa catcaacacc gatgccgtca ccttcaataa
    10201 atggaataat cggattatcc ggaacttgta aagaaccgtc ttggttcagt tgaatagttt
    10261 cgccttgtgg aatttggact tgtgtttgca ttgattttac ccccgtaatg acgaaaaagt
    10321 agtgacgaaa aatgaatgtt gacaatcagc caacaatgga ttaactataa acagtaaatt
    10381 tctaaa
//
LOCUS       DANLXF010000076        10267 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.076, whole genome shotgun sequence.
ACCESSION   DANLXF010000076 DANLXF010000000
VERSION     DANLXF010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10267
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.076"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(87..161)
                     /locus_tag="PXD66_001244"
     tRNA            complement(87..161)
                     /locus_tag="PXD66_001244"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(127..129),aa:Gln,seq:ttg)
     gene            complement(187..271)
                     /locus_tag="PXD66_001245"
     tRNA            complement(187..271)
                     /locus_tag="PXD66_001245"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(235..237),aa:Leu,seq:tag)
     gene            complement(274..350)
                     /locus_tag="PXD66_001246"
     tRNA            complement(274..350)
                     /locus_tag="PXD66_001246"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(314..316),aa:Met,seq:cat)
     gene            complement(517..1341)
                     /gene="aroE"
                     /locus_tag="PXD66_001247"
     CDS             complement(517..1341)
                     /gene="aroE"
                     /locus_tag="PXD66_001247"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005615478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDL6319551.1"
                     /translation="MNRYAVWGNPIAQSKSPRIHQLFALQTQRNIEYIANLGDETAFE
                     QQLADFFASGAKGCNITAPFKERAFKLADEFSETALLAQACNTLKRLEDGRLYADNTD
                     GAGLVSDLERLGWLQKGHKTLILGAGGATKGVLLPLLKAGLEITLYNRTQEKAVVLAE
                     KFAKFGKIQTACLSDLANQLFNLIVNATSLGLQGKYVELPESVIASANIYDMQYAPNM
                     QTPFLNYARRCGATACQDGLGMLVGQAAFAFSLWEGQFPDVEPVLAQLKLEMQTVK"
     gene            complement(1348..1902)
                     /locus_tag="PXD66_001248"
     CDS             complement(1348..1902)
                     /locus_tag="PXD66_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HDL6319552.1"
                     /translation="MNNFLEIVNQLKQEEVVAYPTEAVFGLGCNPQSEKAVRKLLVLK
                     QRPEEKGLILLASDLKYLLPYIDEARMTEQAWQRLSHIGEQAITWIVPVKNTVPSYLT
                     GKFDSIAVRLCNVPAVVQLCEASGVALTSTSANLSGEPPCRNAKEVIDQFGQDFPVLH
                     ADTLGRENPSEIRDIFSQQIFRQG"
     gene            complement(1895..2431)
                     /locus_tag="PXD66_001249"
     CDS             complement(1895..2431)
                     /locus_tag="PXD66_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HDL6319553.1"
                     /translation="MVLFKSTKQTETCPQCGSPLQIKQGKKGLFLGCSAYPECDYIKP
                     LHQAGHIIKQLDELCPECGGNLQLKQGNYGIFIGCSHYPECHFIVHEEAEVEEEFDCP
                     ECKQHKLIARKGRSGKIFYGCIGFPNCKFTLATRPINHLCEKCGCELATIKKIKGKQF
                     TICANKSCQHQTELKPNE"
     gene            complement(2441..2752)
                     /locus_tag="PXD66_001250"
     CDS             complement(2441..2752)
                     /locus_tag="PXD66_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005724113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDL6319554.1"
                     /translation="MNREKITFFSRFEADILSGKKTITIRDKSESYFQPNQELAVFTN
                     ETDRFFANIKVLSVTPIVFDELNEQHAKQENMSLAELKQVIREIYPNDNAFFVIEFTL
                     I"
     gene            complement(2745..3221)
                     /gene="recX"
                     /locus_tag="PXD66_001251"
     CDS             complement(2745..3221)
                     /gene="recX"
                     /locus_tag="PXD66_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDL6319555.1"
                     /translation="MTTSTTKTNKYTAVNYLLYLLSKRDYSEQELRQKLKQKEYELTE
                     IDAAIEKAQANQWQSDERFCTTFIRYRSMQGIGPRRLKQELKLKGVKDWLINQELENA
                     EVDWFELAEQIFEKKRPLVWDLKAKQKMWRFMVSRGFYNDHFSHLMALDYSSDEYE"
     gene            complement(3280..4386)
                     /gene="recA"
                     /locus_tag="PXD66_001252"
     CDS             complement(3280..4386)
                     /gene="recA"
                     /locus_tag="PXD66_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDL6319556.1"
                     /translation="MAEKKSQKNTPVKQIDPEQKEKALAAALAQIEKQFGKGSIMKLG
                     DTQALDIEAVSTGSLGLDSALGIGGLPMGRIVEIYGPESSGKTTLTLSVVAQAQKNGK
                     TCAFIDAEHALDPIYARKLGVDTDGLLISQPDNGEQALEICDALVRSGAVDVIIVDSV
                     AALTPKAEIEGDMGDSHMGLQARLMSQALRKLTANIKATNCLVIFINQIRMKIGVMFG
                     NPETTTGGNALKFYASVRLDIRRSGVVKDGDEVIGSETKVKIVKNKVAPPFREVQFDI
                     MYGEGIARMNELLILAESHGFINKAGAWFSYEGEKIGQGKNNAIKWLKEHPEVASKIE
                     QDIRNLLISNPTFTATPDSENADNADDEFSEEEL"
     gene            complement(4487..5107)
                     /locus_tag="PXD66_001253"
     CDS             complement(4487..5107)
                     /locus_tag="PXD66_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HDL6319557.1"
                     /translation="MCRFPLFETLSIIDGKVQNLQYHQQRFEQATQEYFGCEPRFSLA
                     DILVVPEASQRGKVRCRIDYNATEFELKFFIYSPKKVERFRLVEVENWDYHLKFSDRK
                     RFDLLPILQNEEVIILNNGYVSDCSIGNLLFLKQGIWYSPQHYLLKGTQLSKLLDEGK
                     VVLAKIAKQDLHQYEKIMLINALNPFEEPRALPCSQIYFCDLDRKS"
     gene            complement(5085..6059)
                     /locus_tag="PXD66_001254"
     CDS             complement(5085..6059)
                     /locus_tag="PXD66_001254"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HDL6319558.1"
                     /translation="MQDFIHQANHYGQAKRPFFFLIDFEQQKPLIFPLEQAHASGLFF
                     EFFCKSNLEKPVEKPTKAFELSANPIPFSEYQTGFELVKKEIQAGNSYLLNLSYPTAI
                     ETNYSLAEIFAASKAKYKCYLQGRFVCFSPECFVRIENNRIFSYPMKGTINANEENAE
                     QKLMASEKEFTEHNTIVDLIRNDLALVAKNIEVTKYRYVEKVETHRGAILQTSSEICG
                     ELGENWQENIGSILAKLLPAGSISGAPKEKTVSIIQQAELGKRGYYTGIFGYFDGESL
                     ESAVAIRYIAQTSNGLVFHSGGGITSQSVLTDEYNELLEKVYVPISAI"
     gene            6169..7776
                     /gene="rmuC"
                     /locus_tag="PXD66_001255"
     CDS             6169..7776
                     /gene="rmuC"
                     /locus_tag="PXD66_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HDL6319559.1"
                     /translation="MHFQEIGIYIALAVCVLVGVYLFFLKSRYQRNAYELSQDLGKIT
                     ENFEQIQQKYEVLAQEKNQLEQWAIQQQTKYEAVSERLNERDGQLQRFQQKIELAEQQ
                     ENQLERYINELKERVGSSQAKAESLEEQLQFSQSSLSTKERENQALFSRLNEVQNELT
                     ELRTTLSEKQANFEAQQRNFIEVKQQLNVEFQHLAQQILDEKSKRFSETNQSSLEALL
                     KPFKEQIEGFQKRVNEVHSESLKGSANLEAEIKRVLQIGVSMSEEAQNLATALKGNNK
                     IAGNWGEVQLESALQSAGLLAGEHYQAQESFRDEEGRRFAPDFVVHLPDQKHLIIDSK
                     VSLVAYDQAVRSEENFAISQALDEHCRSLRNHIEGLSKKNYSALLGIKSPDFVLMFIP
                     IEPAYIEAMKHDPQLFNFGYERNVILVSHTTLMPILRTVANLWRIERGNSEAREISEK
                     AGEIYNQVCAVADRLAKLGNTLNTANNQYNQTVTSLVGRQGLLGKVERFQHLSSKASQ
                     IVPQVELIENEVDTIKLEMVSRELLEE"
     gene            7839..8423
                     /locus_tag="PXD66_001256"
     CDS             7839..8423
                     /locus_tag="PXD66_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component II"
                     /protein_id="HDL6319560.1"
                     /translation="MLKKLLIIDNHDSFTFNLVDLLRKLDVPAKVVLVEELDLNEVEH
                     FSHILISPGPDVPRAYLQTFAMLERYHQTKSILGVCLGHQTICEFFGGKLYNLNDVRH
                     GQQRTLTQIEPNPIFKGLPKQLKVGLYHSWGILQNSLQNTPLVPTAVCDQNVLMAFKH
                     QHLPIFGVQFHPESFITEFGQQMLANWLEITQDF"
     gene            8520..9602
                     /gene="prfA"
                     /locus_tag="PXD66_001257"
     CDS             8520..9602
                     /gene="prfA"
                     /locus_tag="PXD66_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDL6319561.1"
                     /translation="MKDSIINKLESLSERHEELQALLGDASIISDQDKFRAYSKEYSQ
                     LEEVVGTFNRWKKLNNDIEEAQLLLDDPDMKDMAAEEIAENKAEIENLEQHLQILLLP
                     KDPNDEYNAFLEIRAGTGGDEAGIFAGDLYRMYSRYCESKRWRIEEMSANESEQGGYK
                     EIIVKISGEGVYGQLKFESGGHRVQRVPKTESQGRIHTSACTVAVMPELPESEMPEIN
                     PSDLRIDTYRSSGAGGQHVNTTDSAVRITHIPTGIVVECQDERSQHKNKAKALAVLAS
                     RIVQVEQERQAAEQADTRRNLLGSGDRSDKIRTYNYPQGRVTDHRINLTVYRLDEVMN
                     GKIDELIQPIITEYQADQLAALSEQN"
     gene            9714..>10267
                     /locus_tag="PXD66_001258"
     CDS             9714..>10267
                     /locus_tag="PXD66_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319562.1"
                     /translation="MRKLGLLAVFTLVLSACSNSTKIEQTILSKQDIVGSWVCAIKYD
                     DLKVSTLDFSEFKANGEMTNFGDISDENFEPIKFTYVTGDKGKWDLKGNRLVIDYDLS
                     ERKVKRTTPKKFLEFLKQEERKASKTAKKLLKYEQGIFNILSDQKNTENSKIILDILK
                     FGHSRMAIQQNMGDKVYSGGCVTA"
BASE COUNT         3082 a         2087 c         2056 g         3042 t
ORIGIN      
        1 aacccggtgc cttaccgctt ggcgataccc caaaagaaaa aactaactta cgttagctta
       61 tatagatttg aatgtttgaa attaagtggc tggggtacta ggattcgaac ctagggatgc
      121 cgagatcaaa acccggtgcc ttaccgcttg gcgatacccc aacactaatt tttgttaatc
      181 gataaatggt gcgggacgag ggacttgaac ccacacacct cgcggcgcca gaacctaaat
      241 ctggtgcgtc taccaatttc gccagtcccg caatggtggc tacagcgaga ttcgaacttg
      301 cgaccccaac attatgagtg ttgtgctcta accagctgag ctatgtagcc atttgcgttc
      361 accatatcgg tcaacggagt gcattatgct gattttaggg ctttccgtca aatacttttt
      421 ttaaaaaatt tgaatttttg attcgcttgt atagttattc gtcgttttgg cttaaatcta
      481 gcattttttg tttataaaat aatagcttat attctgctat ttcaccgttt gcatttctaa
      541 ttttaattga gccagtacag gctcaacatc ggggaattgc ccttcccaca aagaaaacgc
      601 aaacgctgcc tgtccaacca acattcctaa tccgtcttgg cacgctgttg ctccacaacg
      661 ccttgcataa ttgagaaaag gagtttgcat attgggggca tattgcatat cgtaaatatt
      721 cgccgatgct ataacagatt caggcaattc aacatatttg ccttgcagac cgagagaggt
      781 ggcattgacg attaaattaa acagttggtt tgccaaatcg cttaaacaag cggtctgaat
      841 tttgccaaat tttgcaaatt tttcggctaa aacgaccgct ttctcctgcg tgcggttata
      901 tagagtaatc tctaaccccg ctttaagtaa cggaagtaat acgcctttgg ttgctccacc
      961 tgctccaaga attaaggttt tatgcccttt ttgtaaccaa cctagccgtt ccaaatcaga
     1021 aactaaaccg gccccgtcgg tattatcggc atataaccgt ccatcttcta agcgtttaag
     1081 cgtattacac gcctgtgcca acaaagcggt ttcgctaaat tcatctgcca atttaaaggc
     1141 tctttcttta aaaggggcgg tgatgttgca accttttgct ccagaagcga aaaaatcggc
     1201 taattgttgc tcaaaagccg tttcatctcc taagttagcg atatattcaa tgttgcgttg
     1261 ggtttgtaaa gcaaagagtt gatgaatacg gggggattta ctttgagcaa tcgggttgcc
     1321 ccacacggca tatcggttca tttcggctta tccttgtcta aaaatctgtt ggctgaaaat
     1381 atctcgaatt tcggaagggt tttctctacc tagcgtgtct gcgtgtaata caggaaaatc
     1441 ttgaccaaac tgatcgatga cttcttttgc attgcggcaa ggtggttctc cgctcaaatt
     1501 ggcactggta gaggtcaacg caacgccgct tgcctcacac aactgaacca ccgccggcac
     1561 attacataaa cgtacggcaa tagaatcaaa tttgccggtg aggtaacttg gcacagtatt
     1621 tttaaccggt acgatccaag taattgcctg ctcgccaata tggcttaggc gttgccaagc
     1681 ctgttccgtc attcgagctt catcaatata gggaagaaga tactttaaat cagaagccag
     1741 taaaattagc cctttttcct cagggcgttg ttttaatacg agtaatttcc ttaccgcttt
     1801 ttcactttgt ggattgcagc ctaagccaaa aaccgcttcg gttgggtaag ctacaacttc
     1861 ttcttgcttt aattgattaa ctatttctaa aaaattattc attcggtttt aattctgttt
     1921 ggtgctggca acttttgtta gcacaaatgg tgaattgctt gcccttaatt tttttgattg
     1981 tagcgagttc acaaccacat ttttcacaca agtggttaat cggtcgagtt gctaatgtaa
     2041 atttgcaatt agggaagcct atacaaccat aaaatatttt gcctgaacgc ccttttctag
     2101 caatcagttt atgttgcttg cattcagggc aatcaaactc ttcttcaacc tcagcttctt
     2161 cgtgtacgat gaagtggcat tcagggtaat ggctacagcc gataaaaata ccataattgc
     2221 cttgttttaa ttggaggttt ccgccacatt cagggcaaag ttcatctaat tgcttaataa
     2281 tatgaccggc ttgatgtaat ggcttaatat aatcacattc agggtaagct gaacaaccca
     2341 aaaataaccc ttttttgccc tgcttaattt gcaacggcga gccacattgt gggcaagttt
     2401 cggtctgttt ggttgatttg aaaagtacca tattgatttg ctaaataagc gtaaattcaa
     2461 tcacaaaaaa cgcattgtca ttcggataaa tctcccgaat aacttgtttc aattcggcta
     2521 agctcatatt ttcttgttta gcgtgttgct cgtttaattc atcaaacact ataggtgtta
     2581 ccgatagcac tttaatattg gcgaaaaatc tatcggtttc attagtaaaa accgctaatt
     2641 cttggtttgg ttgaaaataa gattctgact tgtctcgaat cgtaattgtt tttttaccgc
     2701 ttaaaatatc ggcttcaaat cggctgaaaa aggtaatttt ctctctattc atattcatcg
     2761 ctactgtaat ccagagccat taaatgacta aaatgatcgt tataaaaacc acggctaacc
     2821 ataaatcgcc acattttctg ctttgctttc agatcccata caagcggtcg ttttttctca
     2881 aaaatttgct cagctaattc aaaccaatcc acttcggcgt tttctaattc ttggttaatt
     2941 aaccaatctt ttaccccttt aagttttagc tcttgcttta aacggcgagg cccaatgcct
     3001 tgcattgaac gataacggat aaaagtggta caaaagcgtt catcactttg ccactgattc
     3061 gcttgagctt tttcaatcgc tgcatcaatc tccgtgagtt catactcttt ttgctttagt
     3121 ttttgccgaa gctcttgctc gctgtaatca cgctttgaaa gtaaatatag cagataatta
     3181 accgctgtgt atttatttgt tttagtagta gatgttgtca tttatcggtt ataaataaaa
     3241 atgcgatttt cacgttgagc gaaaaccgca taaatttaat tagagttctt cttcactaaa
     3301 ttcatcatct gcattgtctg cattttcaga atctggagta gccgtaaaag ttgggtttga
     3361 aatgagtaga tttcgaatat cttgctcaat tttactcgcc acttcagggt gttcttttaa
     3421 ccatttgatg gcattatttt taccctgacc gattttctcc ccttcataag agaaccaagc
     3481 ccctgcttta ttaatgaagc cgtgagattc cgctaaaatg agtaattcat tcatacgggc
     3541 aatgccctca ccgtacataa tatcaaattg gacttcacgg aacggtggag caaccttgtt
     3601 tttcacaatt ttcacttttg tttcgctacc gataacctca tcaccgtctt ttactacgcc
     3661 tgaacgacga atatctaaac gtactgaagc atagaattta agggcattac caccagttgt
     3721 ggtttcaggg ttaccgaaca ttactccaat tttcatacga atttggttaa tgaaaatcac
     3781 caagcagttg gtcgctttga tatttgcggt taatttacgc aatgcttgtg acattaaacg
     3841 agcttgtaag cccatatggg aatcacccat atcgccttca atctctgctt ttggtgtaag
     3901 tgccgcaaca gagtccacaa taattacatc aaccgcaccg gaacgaacta acgcatcaca
     3961 aatttctaat gcttgttcgc cattatccgg ttgtgaaatt aataatccat ctgtatctac
     4021 ccctaactta cgggcgtaaa taggatcaag tgcgtgttcg gcatcaataa aggcacaagt
     4081 tttaccattt ttttgggctt gagccaccac agataaagtt aatgtagttt tacctgaaga
     4141 ctccggtcca taaatttcca caatacgacc cattggtaaa ccaccaatgc ctaatgctga
     4201 gtctaaacca agagaaccgg ttgaaactgc ttcaatatca agggcttgag tatcgcctaa
     4261 cttcataatt gagcctttgc caaattgctt ttcaatttga gctaatgcgg ctgccaacgc
     4321 tttttctttt tgttccggat caatctgttt aaccggagtg ttcttttgac tttttttctc
     4381 tgccatattc tatcctttgt aaacttttat ctaaaagcaa ccgcttgttg ctgcaaaata
     4441 actgtatatt attatagtat ttacttatcc agttgcaagc atttttttag gattttcgat
     4501 caagatcaca aaaataaatt tgcgagcaag gcaatgccct tggttcctca aagggattaa
     4561 gagcattaat caacataatt ttttcatatt gatgcaaatc ttgcttggct attttggcta
     4621 aaacgacttt cccttcatca agcaatttac taagctgagt gcctttgagc aaataatgtt
     4681 gcgggctata ccagattcct tgttttagga acagtaaatt gccgatggag caatcactga
     4741 cgtagccatt attgagaata atcacctctt cattttgtaa aatagggagc aaatcaaacc
     4801 gcttgcggtc gctaaatttg aggtgataat cccaattttc cacctcaacc aagcgaaaac
     4861 gttctacttt tttgggcgaa tagataaaaa atttcagctc aaattctgtt gcgttgtaat
     4921 ctattcggca acgtactttg ccccgttgag aagcctctgg cacaactaaa atatcagcaa
     4981 ggctaaaacg aggttcacag ccaaaatatt cttgtgttgc ttgctcaaaa cgttgttgat
     5041 gatactgtaa gttctgaact ttgccgtcaa taatcgaaag ggtttcaaat agcggaaatc
     5101 ggcacataga ctttctctaa cagttcatta tattcatcag ttaacacact ttgtgaggta
     5161 atgccaccac cactatgaaa cacaagcccg tttgaggttt gagcaatgta acgaattgcc
     5221 accgcacttt ctaagctctc gccatcaaaa taaccaaaaa taccggtgta gtaaccccgt
     5281 tttcctaatt ctgcttgctg aataatcgaa accgttttct ctttcggtgc accgctaatg
     5341 gagccggcag gtagtaattt tgccaatatc gaaccgatat tttcttgcca attttcacct
     5401 aattcgccac agatctcgga acttgtctgc aaaattgccc ctcgatgggt ttctactttt
     5461 tccacataac ggtatttagt aacttcaata tttttcgcca ccaacgccaa gtcatttcgg
     5521 atcaaatcca caatggtatt atgttcggta aactcttttt ccgaagccat taatttctgc
     5581 tcggcattct cctcatttgc attaattgtg cctttcatcg ggtaggaaaa aatgcggtta
     5641 ttttcaattc gcacaaagca ttcaggggaa aaacagacga acctgccttg caaataacat
     5701 ttatattttg ctttgctggc ggcaaaaatt tccgccaatg aataattggt ttcaatggca
     5761 gtcgggtagc tcaaattcag caagtagcta ttgccggctt gaatttcctt tttcactaat
     5821 tcaaacccgg tttggtattc gctaaacgga atcggatttg ccgataattc aaaagcttta
     5881 gtcggttttt caaccggctt ttcgagattc gatttgcaaa aaaattcaaa aaaaagaccg
     5941 cttgcgtgag cctgttctag cggaaaaata agtggctttt gctgctcaaa atcaattaaa
     6001 aagaaaaaag ggcgttttgc ttgcccgtag tgattggctt ggtgaataaa atcttgcata
     6061 gaaaacacaa tggctgaaat tggctgaatc atcaaaagct ctgttataat gcaaagcaat
     6121 agatttgaaa agcatttttt aaaaaaggtt ttaccgatga acaacaccat tcattttcaa
     6181 gaaattggga tctacatcgc ccttgccgtt tgtgtgctgg taggcgttta tctctttttc
     6241 ttgaaatccc gttatcagcg aaatgcctac gaattaagcc aagatcttgg caaaatcacc
     6301 gaaaattttg aacaaattca gcaaaaatat gaggtgttgg ctcaagaaaa aaatcagctt
     6361 gagcagtggg caattcaaca acagaccaaa tatgaagcag tgagtgaacg tttaaacgag
     6421 cgagatgggc aattgcaacg attccaacaa aaaatcgaac ttgccgaaca gcaagaaaac
     6481 cagctggaac gctatattaa tgagctaaaa gaacgtgtgg gttcttccca agcaaaggca
     6541 gaaagtttgg aagaacagct acaatttagc caaagttcac tctcaactaa agagcgggaa
     6601 aatcaagcgt tatttagccg tttaaacgag gtacaaaacg agctgaccga actgcgtact
     6661 acgctttcgg aaaaacaggc aaattttgaa gcacaacaac gcaattttat tgaggtaaaa
     6721 caacagctta atgtggaatt tcaacatttg gcacaacaaa ttcttgacga aaaaagcaag
     6781 cgttttagcg aaaccaatca aagctcgtta gaagctctac tgaagccgtt caaagagcaa
     6841 attgaaggct ttcaaaaacg ggtgaatgag gtacatagtg agtcgctcaa aggctcggca
     6901 aatttagagg ctgaaattaa gcgggtgcta caaattggcg tttcaatgtc ggaagaggca
     6961 caaaatctgg caacggcatt aaaaggcaat aataaaattg ccggcaactg gggtgaagta
     7021 cagcttgaaa gtgccttaca atctgccggt ttactcgcag gcgagcatta ccaagcccaa
     7081 gagagttttc gtgatgaaga ggggcgacgt tttgcccccg attttgtggt gcatttgccg
     7141 gatcaaaaac atctgattat tgacagtaaa gtctcgttag tagcgtacga tcaagcggtc
     7201 agatcagaag aaaattttgc aatttctcaa gccttagacg aacattgccg ttcgctacgc
     7261 aatcatattg agggattgtc taagaagaac tacagtgcat tattaggcat taaaagcccg
     7321 gattttgtac tgatgtttat tccgatagaa ccggcttata ttgaagcgat gaagcacgat
     7381 ccgcaactgt ttaactttgg ctatgaacgc aatgtgattt tggtttctca caccacctta
     7441 atgccgattt tacgcacggt cgccaattta tggcggattg aacgaggcaa cagcgaggct
     7501 cgtgaaatca gcgaaaaagc cggcgaaatt tacaaccaag tgtgtgcggt tgccgatcgg
     7561 ttagctaaat taggtaacac gctaaatacc gcaaataatc aatacaatca aaccgttacc
     7621 tcgttagtgg gacggcaagg cttactgggc aaagtggaac gattccaaca tctttcaagc
     7681 aaagcaagcc aaattgtgcc gcaggttgag ctgattgaaa atgaggtgga tactattaaa
     7741 ttggaaatgg tatcgagaga attattggaa gaatagtcaa atctctttat tccctacaaa
     7801 aatatggggt gatttgaaat actaaagaaa ataataaaat gctaaaaaaa ctattaatca
     7861 tcgacaacca cgactctttt accttcaact tggtggattt actccgtaag cttgatgtgc
     7921 cggcgaaagt ggtgctggtt gaagagcttg acctcaatga ggtggagcat tttagccata
     7981 ttttgatttc gcccggccct gatgtgccga gagcctatct acaaaccttt gcgatgttgg
     8041 agcgttatca tcaaaccaaa tcaattctag gcgtttgctt ggggcatcaa acgatttgcg
     8101 agttttttgg cggcaagttg tataacctaa acgatgttag acacgggcag caacgcacgc
     8161 tcactcaaat tgagcctaac cccatattta aaggtttgcc taagcagttg aaagttgggt
     8221 tgtatcactc ttggggcatt ttgcaaaatt cgttgcaaaa tacaccgctt gtaccaaccg
     8281 cagtttgcga tcaaaatgtg ttaatggcgt ttaaacacca acatttgccg atttttggcg
     8341 tgcagtttca cccggaatct tttatcactg aatttggtca acaaatgttg gcaaattggc
     8401 ttgagattac acaagatttt tagcgtgaaa tccgctataa tccgtcagtt atttaccctt
     8461 ttaaaatctg taggggcgaa aaatttttcg ccttacttaa tatgcaagag ataatttaaa
     8521 tgaaagattc gattatcaat aaattagaaa gtttaagtga acgccacgaa gaactacagg
     8581 cgttacttgg cgatgcctca attatttccg atcaagacaa attccgtgct tattccaaag
     8641 aatattcgca attagaagaa gtggttggca cctttaaccg ctggaaaaag ctgaacaatg
     8701 atattgaaga ggctcaatta ttactcgatg atcctgatat gaaggatatg gcagcggaag
     8761 aaattgcgga aaataaagcc gaaatcgaga atcttgagca acatttacaa attttattat
     8821 tgccgaaaga tccgaatgat gaatacaacg ccttccttga aatccgtgcc ggaacgggcg
     8881 gtgacgaggc tggtattttt gccggtgatt tataccgtat gtacagccgt tattgcgaaa
     8941 gtaaacgctg gcgaattgaa gaaatgtcgg caaatgagag tgaacaaggc ggatataaag
     9001 agatcatcgt taaaatttca ggcgaaggcg tttacggtca gcttaaattt gaatcgggcg
     9061 gacaccgtgt acaacgtgta ccgaaaaccg aatcacaagg gcgtattcac acctctgctt
     9121 gcacggtagc ggtaatgccg gagttgccgg aaagcgagat gccggaaatc aatccatctg
     9181 atctgcgaat tgacacctac cgctcatcgg gtgcaggcgg tcagcacgtt aataccaccg
     9241 actctgcagt gcggattact catatcccga ccgggattgt ggtcgagtgt caagatgagc
     9301 gttcacagca caaaaataaa gccaaagcgc tggcagtgtt agcctctcgg attgtacaag
     9361 ttgagcaaga acgccaagcc gcagagcaag cggatacccg ccgtaactta cttggctcag
     9421 gcgaccgttc ggataaaatc cgcacctaca actacccgca agggcgtgtg actgatcacc
     9481 gtattaacct cacggtttat cgtttagacg aagtgatgaa cggcaaaatt gatgaattga
     9541 tacaaccgat tatcactgaa tatcaagcgg atcagttagc ggcgttgtcg gagcaaaatt
     9601 aattttattg taagggtaca ctgtgtgtgc ctgccaaata taaatttgca aaattttgac
     9661 tatttttgat cgcttgtagt ttctaaaaag tttaaaaatt tgaggaaaac aaaatgagaa
     9721 agttaggttt attagcagta tttactttag tattatcagc ctgttcaaat tcaacaaaaa
     9781 ttgaacaaac cattctttcc aagcaggata tcgtaggctc ttgggtatgc gctattaaat
     9841 acgatgatct aaaagtaagc actttagatt tttctgaatt taaagctaat ggcgaaatga
     9901 cgaatttcgg tgatatttct gatgagaatt ttgagcccat taaatttact tatgtaacag
     9961 gagataaagg gaagtgggac ttaaaaggaa atcgattagt gattgattat gatctttctg
    10021 aacgtaaagt aaaaagaaca acaccaaaaa aatttttaga gttcttgaaa caggaagagc
    10081 gtaaagcctc aaaaacagct aaaaaactcc ttaaatatga acaagggatt ttcaatattc
    10141 tgagtgatca gaaaaatact gaaaacagta aaataatatt agatattttg aagtttggtc
    10201 attcaagaat ggcgattcag caaaatatgg gagacaaagt ttattcaggt ggttgtgtaa
    10261 cagctga
//
LOCUS       DANLXF010000077        10203 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.077, whole genome shotgun sequence.
ACCESSION   DANLXF010000077 DANLXF010000000
VERSION     DANLXF010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10203
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.077"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            59..313
                     /gene="rsfS"
                     /locus_tag="PXD66_001259"
     CDS             59..313
                     /gene="rsfS"
                     /locus_tag="PXD66_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDL6319563.1"
                     /translation="MLAIDVKGKSSITDTMILATGTSSRHVASTAERLITEAKQAGFE
                     VFGDEGKAVADWIVVDFGKAMVHLLQSDSREMYQLEKLWA"
     gene            390..857
                     /gene="rlmH"
                     /locus_tag="PXD66_001260"
     CDS             390..857
                     /gene="rlmH"
                     /locus_tag="PXD66_001260"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010618862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDL6319564.1"
                     /translation="MKIQLIAVGTKMPAWVTTGFEEYQRRFPKEMPFELVEIPAGKRG
                     KNADTKRILELEGKAMLAAAGKAKIVTLDIPGKAWTTEQLATQLEGWKNDGRDVALLI
                     GGPEGLSPECKAAAEQSWSLSPLTLPHPLVRVVVAESLYRAWSVTINHPYHRE"
     gene            881..2830
                     /gene="mrdA"
                     /locus_tag="PXD66_001261"
     CDS             881..2830
                     /gene="mrdA"
                     /locus_tag="PXD66_001261"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDL6319565.1"
                     /translation="MFGKLAKFTASNQREKVRDGQAESNLYARRALVAFLGVLALSGV
                     LLTNLYHLQVVNYEDYQTRSNGNRIKLLPVPPARGLIYDRNGKVLAENLTYFGLFIVP
                     EKTENIEQTLTELKDIVGLTDEDIENYHKEKKRSSRYTPIMLKGNLNEAQMARFAVNQ
                     YRFPSLDVQAYYKRNYPYGELLTHIIGYVAKINENDKKKLQEAGKFGNYAGSHDMGKL
                     GIEKFYEDQLHGVTGFEEVEINNRGKVIRKLRDQAGVAGSSIRLSIDVELQQYVMSLI
                     AKHKGAVVVLDPRDSSILAMVSNPSYDNNLFVGGISGQDYKALLDDPTRPLYSRTTQG
                     TYPPASTIKPFMAVAGLEEGMITPSTTISDPGYWILPGTTQRYRDWKRSGHGATNVNK
                     AITESSDTFFYQLAYKMGIDKMSDWMKKFGFGVKTGIDIFEESAGVMPTREWKQKRYK
                     KPWVQGDTIPVGIGQGYWISTPLQLAKATAVLINNGRVNTPHLMLEVQSSNSVTPYQD
                     PLLYEDIKGVPDVYWNLAKQGMYNVNHAANGTARKVFAGAFYHSAGKTGTAQVFNLNG
                     KNYDKNAIKKELHDHAWFIGYAPYEQPKIVVALILENAGGGGSAAAPVARQIMDYALR
                     KGIGEQPDSQNSEQNKPLVTEEHHE"
     gene            2823..3950
                     /gene="rodA"
                     /locus_tag="PXD66_001262"
     CDS             2823..3950
                     /gene="rodA"
                     /locus_tag="PXD66_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HDL6319566.1"
                     /translation="MNSGFKKLFWKIFSLDLLLLSGLLAITGYGLLVLYSASGANERM
                     FTSRLIQVSLGLGLMLIMAMVPPRFYQKVSPYLYIFCLILLILVDLVGETSKGAQRWL
                     NLGFVRFQPSEIAKLAVPLMVATYLGKRPLPPSFKDTFIALAIIIVPTLLVAIQPDLG
                     TSILVCSAGIFVLFLAGLSWKLIGAGVVFLAGFIPIMWFFLMHDYQKTRVMTLIDPNK
                     DPLGAGYHIIQSKIAIGSGGINGKGWMEGTQSQLEFLPEPHTDFIFAVLSEEHGMIGV
                     LILLAIYLFIIARGLVIGTKSDSAFGRILSGGTALLFFVYVFVNIGMVSGILPVVGVP
                     LPLFSYGGTSYVTLMAAFGLMMSTYVHRKRVGSNNPYDKLK"
     gene            4042..4701
                     /locus_tag="PXD66_001263"
     CDS             4042..4701
                     /locus_tag="PXD66_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HDL6319567.1"
                     /translation="MRLGKIVTLLLAGFLTFSSLNSLAATKNTKAKSSILAKKQATSK
                     AIKKVGISKVKKKEVVAKATKKKTISTVTTKPIKSTKKLARKVVKKTVTAKKKLVKKQ
                     HKPLVKKHFQTGVASYYANYFNGRRTANGETFNNAKMTAAHRTLPFGTLIEVTNLRNG
                     RSVVVRVNDRGPYAHARVLDLSSAAAKKIGMHHSGTARVKIAIVDKSKLGSTNQFYVM
                     N"
     gene            4715..5893
                     /locus_tag="PXD66_001264"
     CDS             4715..5893
                     /locus_tag="PXD66_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HDL6319568.1"
                     /translation="MKKTLVKAMSVVGFAVSSIAYADLNTNFNLSAPQINAQTYILMD
                     YNSGEVLVSLNPDQRQYPASLTKMMTSYVVGEALKSGKVQNSDMVIVTENSWAQKFPG
                     SSLMFLDLNSKVSVGELMKGLIIVSGNDASVALAEHVSGSQAAFINEMNKYAQQFGLK
                     NTHFTTVHGLDDPEQYSSARDMAIIGSHIIRDQPEEYKIYAEKEFQYNIKKPQPNRNG
                     LLWDKTIQVDGMKTGHTDKAGYNLVASAINPNTRLISVVMGVPTYKGREVESKKLLQW
                     GFANFETIKSIPAAQSVAEQSVYYGEVNKVQLGSVQDSFVTIPKGRSADLKARYELEK
                     KNLEAPLAKGQVVGKMIYQLDGKDVASTNLQVLQDVPEAGIFGKAWDWIALTVKSLFD
                     "
     gene            6061..6360
                     /locus_tag="PXD66_001265"
     CDS             6061..6360
                     /locus_tag="PXD66_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HDL6319569.1"
                     /translation="MSQPKAINLQDLPQAKLKDLLEFPCHFTFKVVGANREDLVDDVV
                     VVTQKYAKGDYNPRQQKSSKGTYNSVSIDIVAENIEQVETLYTELAKINGVRMVL"
     gene            6422..7075
                     /gene="lipB"
                     /locus_tag="PXD66_001266"
     CDS             6422..7075
                     /gene="lipB"
                     /locus_tag="PXD66_001266"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HDL6319570.1"
                     /translation="MKLIIRQLGIQPYEEIWHQMQSFTDSRDGNTDDEIWLVQHPAVF
                     TQGSAGKPEHLLNPTNIPVVQSDRGGQITYHGEGQQIMYVLIDIKRLKSLGKEVSVRD
                     LVTALEQTVVQTLAEYGIESYPKADAPGVYVDGKKICSLGLRIRKGCSFHGLALNINM
                     DLTPFQNINPCGYAGLEMCQLSELVHRPDLRCEEVAPKLVAHFSTILGYNNQQIINN"
     gene            7090..8097
                     /gene="lipA"
                     /locus_tag="PXD66_001267"
     CDS             7090..8097
                     /gene="lipA"
                     /locus_tag="PXD66_001267"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HDL6319571.1"
                     /translation="MATSTAQTTATATVKKMSPFKMEKGVKYRDAAKTSVIQVRNIDP
                     DQELLKKPEWMKIKLPANSAKIDSIKNGMRRHGLHSVCEEASCPNLHECFNHGTATFM
                     IMGAICTRRCPFCDVAHGKPLPLDPDEPRKVAETVQDMKLKYVVITSVDRDDLPDRGA
                     AHFSATVREIKALNPHCKVEILVPDFRGRVEQAVAILKENPPDVFNHNLENVPRLYRE
                     VRPGADYKWSLELLKIFKQEFPHIPTKSGLMVGLGETNEEILEVMQDLRDHGVTMLTI
                     GQYLQPSRHHLKVERYVPPAEFDMFREEAEKMGFEHAACGPFVRSSYHADLQAKGELV
                     K"
     gene            8284..9387
                     /locus_tag="PXD66_001268"
     CDS             8284..9387
                     /locus_tag="PXD66_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL6319572.1"
                     /translation="MKKLLLNSVFAATLLAFGATANAEIKTITDVLGREVKVDVPAKR
                     VALTFYYPDYIAVTGVENFDKVVGISREFWEKFNPGSWALFAEKMPNLKNIADLGYVN
                     SGTFSTEKTIALKPDVLVLPEIQYQALASEISRIEQAGIPVVVDFNKQTIENHTKSVR
                     VFGQLTGTEERAEKIAKEYEEGIADIQSCIDNAKVSKPKIYAEFGNKGPKEHSFTFGK
                     NMWGAIAETVRGDNISAPFVENWGQINPEQVLVSKPDVIMISGTELGNDTNPEIMSMG
                     INIEEADAQKRLKGFIERTGWKELPAVKSGRVYGLYHTASRSISDLASAQFMAKALYP
                     ELFKDIDPEKTYLDFHNNYLPITPKGTFFMQLK"
     gene            complement(9478..>10203)
                     /locus_tag="PXD66_001269"
     CDS             complement(9478..>10203)
                     /locus_tag="PXD66_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL6319573.1"
                     /translation="SVVADKINLHFEQGKIYTLLGPNGAGKSSLLKTLFGEIAHQGTI
                     DFDGNKLTSSHLHSWRKEIGYMPQDTQVEASLSALEVILLGQLDSLNMYISDELLNEA
                     VDIMDKLGISHLAHQDIMRLSGGQRQMVMFAQVLLRKPHILLLDEPVSALDMYHQINL
                     LEYVNAYTRANNLITIMVLHDLSLAAQFSDSVMLLSEGKFKHKAELVICWNAILFRDS
                     IMSILRFYMMETARRLSARFANV"
BASE COUNT         3083 a         1908 c         2369 g         2843 t
ORIGIN      
        1 ggaacagaat ttagtcgaat ttttaaccaa aaccttagat gatttaaagg cacaagatat
       61 tttagccatt gatgtgaaag ggaaatctag cattactgat acgatgattt tagccaccgg
      121 tacatcgagc cgtcacgttg cttcaacggc agagcgttta attacggaag caaaacaggc
      181 tgggttcgag gtatttggag atgaaggtaa agcagttgcc gattggatcg tggtcgattt
      241 tgggaaggca atggttcact tgttgcaatc cgatagccgt gagatgtacc aattagagaa
      301 actttgggcg tagcagttca ataaggaacg acaagcggtg atttttgcaa cattttttgc
      361 aaattaaacc gcttgtaagt gaaagagaga tgaaaattca attaattgcg gttggcacta
      421 aaatgccggc gtgggtaacg acagggtttg aagagtatca acgccgtttt ccgaaagaga
      481 tgccgtttga gttggtggaa attccggcag gaaaacgggg caagaatgct gatactaaac
      541 gcattcttga gcttgaaggc aaagcaatgt tggcggcagc gggcaaggca aaaatcgtga
      601 ctttggatat tccgggcaaa gcgtggacaa ccgagcagtt agccactcag ttagagggct
      661 ggaaaaacga tgggcgagat gtagcgttat tgattggcgg cccggaagga ttatcaccgg
      721 aatgcaaagc cgctgccgag cagagttggt cgttgtcgcc tttaacctta cctcacccac
      781 tggtgcgggt ggtggtggcg gaaagtttgt atcgggcgtg gtcggttacc attaatcacc
      841 cttatcatcg agagtagtga gtcattttag atagtgttat atgtttggta aattagcaaa
      901 attcacagct tcaaaccagc gagaaaaggt gcgagacggg caggcagaaa gcaatctgta
      961 tgcccgccgt gccttagttg cgtttctggg ggtgttggcg ttaagtggtg ttttattaac
     1021 caacttgtat cacttgcaag tggtgaatta tgaggactac caaacccgtt caaacggtaa
     1081 ccgcattaaa ttgttgcctg tgccaccggc tcgaggctta atttatgacc gtaacggtaa
     1141 agtattggcg gaaaatttaa cctatttcgg gctgtttatt gtgcctgaaa agacggaaaa
     1201 cattgagcag actttaaccg agttgaagga tattgttggg ctgacggacg aagatatcga
     1261 gaattatcat aaagagaaaa aacgcagttc tcgctatacc ccaattatgc tcaaaggcaa
     1321 tttgaatgag gctcagatgg ctcgttttgc ggtgaaccaa taccgttttc caagtttaga
     1381 tgtgcaggcg tattataagc ggaattatcc ttacggcgag cttttaaccc atattatcgg
     1441 ttatgtggcg aaaattaacg aaaacgataa gaaaaagctg caagaagccg ggaaattcgg
     1501 caattatgcc ggttcgcacg atatggggaa attggggatt gaaaaattct atgaagatca
     1561 gctccacggt gtcaccggtt ttgaagaggt agaaattaat aaccgaggga aagtaatccg
     1621 taaattgcgt gaccaagcag gggttgccgg tagcagtatt cgcttgagta ttgatgtgga
     1681 attacagcaa tatgtgatgt cgttaattgc taagcataaa ggggcggtgg tggtgcttga
     1741 ccctcgtgat agcagtattt tagcgatggt ctcaaaccca agttatgaca ataatttatt
     1801 tgtgggcggt atttcagggc aggattataa agcattattg gacgatccaa cccgaccact
     1861 ttatagccgt actacacaag gcacttatcc accggcttca actattaagc catttatggc
     1921 ggtggcaggt ttggaagagg gaatgattac cccttctacc accatttcag accccggcta
     1981 ttggattttg cccggcacta cacaacgtta tcgagattgg aaacgtagcg gacacggtgc
     2041 aactaatgtg aataaagcaa ttaccgaatc gtctgatacc tttttctatc aattagccta
     2101 taaaatgggg attgataaaa tgtcggattg gatgaaaaaa ttcggtttcg gagtgaaaac
     2161 cggtatcgat atttttgagg aatccgcagg ggtaatgcca acccgtgaat ggaaacaaaa
     2221 acgctataaa aaaccttggg tgcagggcga tactattccg gttgggattg ggcagggcta
     2281 ctggatttcg accccgttac agttggcgaa agccacggcg gtgttaatca acaacgggcg
     2341 ggtgaataca ccgcatttaa tgcttgaagt gcaaagcagt aattcggtta cgccgtatca
     2401 agacccattg ttgtatgaag atattaaagg cgtgccggat gtctattgga atttagcaaa
     2461 acagggaatg tataacgtaa accacgccgc aaacggtacg gcacgaaaag tgtttgccgg
     2521 tgcattttat cattcagcag ggaaaaccgg tacggctcag gtgtttaact taaacggtaa
     2581 aaattacgat aaaaatgcga ttaaaaaaga gttgcacgac cacgcttggt ttatcggcta
     2641 tgctccttat gaacaaccta aaatagtggt ggcattaatt ttagaaaatg caggcggtgg
     2701 tggtagtgcc gcggctcctg ttgctcgtca gattatggat tatgctttga gaaaaggcat
     2761 tggtgaacag ccggattcgc aaaattctga gcaaaataaa ccgcttgtca cagaggaaca
     2821 tcatgaatag tggctttaaa aaacttttct ggaaaatttt ttcactcgat ctccttttat
     2881 tatcaggatt attagctatt accggttatg gtttattagt actatatagc gcaagcggtg
     2941 caaatgagcg gatgtttacc agtagactga ttcaagtttc gctagggctt gggctaatgt
     3001 tgataatggc aatggtacct cctcgctttt atcaaaaagt ttcgccttat ctgtatattt
     3061 tctgtctgat attattgatt ttggtggatt tggtcggcga aaccagtaaa ggagcgcaac
     3121 gttggttgaa tttaggattt gttcgctttc agccttctga gattgccaag ctcgctgtgc
     3181 cactgatggt agccacttat ttaggtaagc ggccgcttcc tccgagtttt aaagatactt
     3241 ttatcgcctt agccattatt attgtgccaa ccttattggt ggcaattcag cccgatttgg
     3301 gaacatctat tttagtgtgt tctgccggta tttttgtttt atttttggca gggctaagtt
     3361 ggaaattaat tggcgcaggt gtggtgtttt tagccggttt tatcccgata atgtggtttt
     3421 tcttaatgca cgattatcaa aaaacccggg tgatgacctt aattgatcct aataaagacc
     3481 cgttgggtgc cggctatcat attattcaat ccaaaattgc gataggctct ggtggcatta
     3541 atggaaaagg ctggatggaa ggaacgcaat cccagcttga atttttacct gaaccgcata
     3601 ccgattttat ttttgcagtg ttaagcgaag agcatggtat gataggtgtg ctgattttat
     3661 tagcgattta tttgtttatt attgcccgag gtttagtgat tggcacaaaa tcggattctg
     3721 ctttcgggcg tatattatca ggtggtacag cccttctatt ttttgtttac gtttttgtaa
     3781 atattggtat ggtgagtggt attttacccg tagtcggtgt accattaccg cttttcagtt
     3841 acggcggaac ctcctatgtg acgctgatgg cggcatttgg tttgatgatg tcaacctatg
     3901 ttcaccgtaa gcgtgtaggt agtaataatc cttatgataa actgaaataa attaaacttt
     3961 tgcttttttg cggtatcaaa aagcagtgaa acgatcatcg ttacaaacaa taacgacatc
     4021 cacatagagg aaaaaactta tatgagatta ggtaaaattg taaccctgtt gttagctgga
     4081 tttctgacat ttagttcatt aaattctttg gctgcgacaa agaatacaaa agctaaatcg
     4141 agtattttag ccaagaagca agcaacttct aaagcaataa aaaaagtagg tatttctaaa
     4201 gttaagaaaa aagaagtagt ggctaaagct acaaaaaaga aaacaatttc tactgttaca
     4261 actaagccga ttaaatctac aaaaaagctg gctcgcaagg ttgtcaagaa aacggtaacc
     4321 gctaagaaaa aattggtaaa aaagcagcat aagccactgg tcaaaaaaca tttccagact
     4381 ggtgttgcca gctattatgc aaattacttt aatggtcgcc gaaccgctaa tggggagact
     4441 ttcaataacg caaaaatgac ggctgcacat cgaactttac catttggtac tttaattgaa
     4501 gtgacgaatt tacgaaatgg tcgttctgtg gtggtgcgtg taaatgatcg cggaccttat
     4561 gctcacgcac gggtcttgga tttatcatca gccgcagcga aaaaaatcgg tatgcaccat
     4621 tcggggacgg caagagtgaa aattgcgatt gttgataagt caaaattggg atcgactaat
     4681 cagttttatg taatgaatta atttaagagg gaatatgaaa aaaacattag tgaaagcgat
     4741 gagcgtggta ggttttgcgg tttcaagcat tgcctatgcg gatttaaaca ctaacttcaa
     4801 tttatcggca ccccagatca acgcacaaac ctatattttg atggattaca actccggtga
     4861 agttttagtg agcctaaatc cggatcaacg tcaatatcct gcatcattga ccaaaatgat
     4921 gactagctac gtagttggtg aagccctaaa atcgggaaaa gtgcaaaaca gtgatatggt
     4981 gatagtcacc gaaaattcgt gggcgcaaaa attccccggc tcttcattaa tgtttttaga
     5041 tttaaacagc aaagtttcag taggtgagtt gatgaaaggg ctgattatcg tctcgggtaa
     5101 tgatgcctct gtggcgcttg ctgaacatgt ttcaggctca caagcggcgt tcattaatga
     5161 aatgaacaaa tatgcccaac agtttggctt aaagaatact cactttacca ctgttcacgg
     5221 tttagatgac cctgagcaat attcttctgc tcgtgatatg gcgattattg gttcgcatat
     5281 tattcgtgat cagccggaag agtataaaat ttatgcggaa aaagagttcc aatataacat
     5341 caaaaaacca caaccaaacc gcaacggatt attgtgggat aaaaccattc aagttgatgg
     5401 tatgaaaaca gggcatactg ataaagccgg ctataactta gtcgcatcag caattaaccc
     5461 gaatacacgt ttaatttctg tagtgatggg cgtgccaact tataaagggc gtgaagtgga
     5521 aagtaagaaa ttactgcaat ggggctttgc gaactttgaa accattaaat ctattccggc
     5581 agctcaatcg gtggcagagc aaagtgtcta ttacggtgaa gtcaataaag tacaattagg
     5641 ctcggtgcaa gatagctttg tgactatccc gaaaggtcgc tcggcagatt taaaagcccg
     5701 ttatgagtta gagaagaaaa acctcgaagc tcctttagca aaagggcagg tagtgggtaa
     5761 aatgatctac caacttgacg gcaaagatgt tgcaagcact aacttacaag tcttacaaga
     5821 tgtaccggaa gccggtattt tcggcaaagc gtgggattgg attgccctta ctgtgaagag
     5881 cttgttcgat taatcttgtt atttgccttt ttatccccat ctagggagca atgcaagcgg
     5941 tgtgattttg caaaaaattt gcaaaatttc accgcttacc gttttagata tgaatgtata
     6001 aaaagtgtag tggcgaaaaa tttttcgccc ctcgaagaaa gaatagaaga aggatcaaaa
     6061 atgtctcaac ccaaagcgat aaacttacaa gatttacctc aagccaaatt aaaagatctg
     6121 ctagagtttc cgtgccattt cacgttcaaa gtggtgggag caaaccgaga agatttggtt
     6181 gatgatgtgg tagtggtcac gcaaaaatat gccaaaggcg attacaatcc acgtcagcaa
     6241 aaaagctcaa aaggtaccta taattccgtt tcgattgata ttgtggcgga aaatattgag
     6301 caagtagaaa ctctctacac ggaacttgcc aaaattaatg gcgtaagaat ggtattataa
     6361 tgcgaggggc gaatgacatt cgccctcaat ttttcaataa agcgatgttg gaaaatagaa
     6421 aatgaaatta ataattcgcc aactaggcat tcagccttat gaagaaattt ggcaccaaat
     6481 gcaatctttc acagatagcc gtgatgggaa taccgatgat gagatctggc tggttcagca
     6541 cccggctgtg tttacgcaag gatcggcagg caaaccggag catttgttaa atccaaccaa
     6601 tattccggtg gtgcaaagcg accgtggcgg tcaaattact taccacggcg aggggcagca
     6661 aattatgtat gtgctgattg atatcaaacg gctgaaatcc ttgggtaaag aagtgtcggt
     6721 tcgagattta gtgactgccc tggagcaaac cgtggtacaa acgttggcag aatatggcat
     6781 agaaagttat ccaaaagccg atgcacccgg ggtgtatgtg gacggcaaga aaatctgctc
     6841 acttggttta cgaattcgaa aaggttgctc tttccacggt ttggcattaa atatcaatat
     6901 ggacttaacg ccttttcaaa acatcaatcc gtgcggttat gcagggctgg aaatgtgcca
     6961 attaagtgaa ctggttcatc gccctgattt gcgttgcgaa gaggttgctc caaaattagt
     7021 ggctcatttc agcacaattt taggctataa taaccaacaa attattaaca attaggataa
     7081 aacacagtaa tggctacatc gacagctcaa acgacggcaa ctgctacagt gaaaaaaatg
     7141 agccctttca aaatggaaaa aggggtgaaa tatcgtgatg ccgccaaaac ctctgtgatt
     7201 caggtgcgta atatcgaccc ggatcaagaa ctcttaaaaa aaccggagtg gatgaaaatc
     7261 aaactgccgg caaattcggc aaaaattgat agcattaaaa acggtatgcg tcgccacggc
     7321 ttgcactcgg tgtgcgagga agcctcttgc ccgaatttac acgaatgttt taaccacggt
     7381 actgcaacct ttatgattat gggggcaatc tgtacccgtc gttgcccgtt ctgtgatgtg
     7441 gcacacggca aaccgcttcc gcttgacccg gacgaacctc gcaaagtggc agaaacggtg
     7501 caagatatga aactcaaata tgtggtgatt acctcagtgg atcgtgatga tttgcccgac
     7561 cgtggtgcgg ctcatttctc tgcgaccgtg cgtgaaatca aagccttaaa tccgcattgt
     7621 aaggttgaaa ttttagtgcc tgatttccgt ggacgtgtgg agcaagcggt ggcaattctc
     7681 aaagaaaatc cacctgatgt ctttaaccac aacctagaaa atgtgccacg tctctatcgt
     7741 gaagttcgtc cgggagcgga ttacaaatgg tcgttggaat tattgaaaat cttcaagcaa
     7801 gagttcccgc atattccgac caaatccggc ttaatggtag gcttaggtga aaccaacgaa
     7861 gaaatcttgg aagtaatgca agatttacga gaccacggcg tgacgatgct caccatcggg
     7921 caatacttac aaccaagccg ccaccactta aaagtggaac gttatgtgcc accggcagag
     7981 tttgatatgt tccgtgagga agctgaaaaa atgggctttg aacacgcagc ctgcggaccg
     8041 ttcgtgcgtt cttcttacca cgcggattta caagcgaaag gcgaattggt gaaataaaat
     8101 cgataagggc gaatattatt tgccctttgt ttttgtggtt tgcaaagttt tagcaaaatt
     8161 aaaccgcttg taatctcttc taatttactc ttctatagat aatttcgttg ttgcgaatta
     8221 ttttcattga ctatttgtta tagaaagtta caattcaact ttccatttct ttttggagtt
     8281 ccaatgaaaa aattactatt aaacagcgta tttgctgcca cattgcttgc ttttggtgca
     8341 actgccaatg ccgaaattaa aaccatcact gatgtattag ggcgtgaggt caaagttgat
     8401 gtgcctgcta aacgtgttgc actgactttc tattaccctg actatattgc ggtaaccggt
     8461 gttgaaaatt tcgataaggt agtagggatt tcacgagagt tttgggaaaa gttcaaccca
     8521 ggcagttggg cattatttgc agaaaaaatg ccaaatctaa aaaacattgc cgatcttggc
     8581 tatgtgaata gtggtacttt ttcaacagaa aaaaccatcg cattaaaacc agatgtgtta
     8641 gtgctacctg aaattcaata tcaagcgtta gccagtgaaa tttcacgcat tgagcaagct
     8701 ggcattcctg ttgtagtaga tttcaacaag caaactattg aaaaccatac aaaaagcgtg
     8761 agagttttcg gtcagcttac aggcactgaa gagagagcgg agaaaattgc caaagagtat
     8821 gaagagggca ttgctgatat tcagagctgt attgataatg caaaagtaag taagccgaaa
     8881 atctatgctg aatttggtaa taaaggcccg aaagagcata gttttacctt tggtaaaaat
     8941 atgtggggag cgattgccga aacggtgcga ggcgataaca ttagtgcccc gtttgtagaa
     9001 aactgggggc agattaaccc tgagcaggtg ttagtttcta aacctgatgt gattatgatt
     9061 tcaggtacgg aactaggcaa cgacactaac cctgagatta tgtcgatggg gattaatatt
     9121 gaagaagccg atgctcaaaa acgcttaaaa ggctttattg agcgtacagg ttggaaagag
     9181 ttacctgcgg taaaatcagg gcgtgtttat ggtttatatc atacagcttc tcgctcaatt
     9241 tcagatttag cgagtgctca atttatggca aaagcacttt atcctgaatt gtttaaagat
     9301 atcgatcctg aaaaaacgta tttggatttc cacaacaact acttaccaat tacgccaaaa
     9361 ggcacattct ttatgcaact gaaataaaaa taacaagcgg tcattttgca gatttatttt
     9421 gcaaaatgac cgcttgtatt cgcttttact atttgcggtt caaacagcaa tcaatattta
     9481 aacatttgcg aagcgggcgg ataatcggcg agccgtttcc atcatataaa atctcaatat
     9541 tgacattata gagtctctga ataatatcgc attccaacat atcacaagct ctgccttgtg
     9601 cttgaatttc ccctcgctta atagcatcac actatccgaa aattgagccg caaggcttaa
     9661 atcgtgcagc accataattg taattaggtt attcgctcga gtgtaggcat tcacatattc
     9721 aagtaagttg atttgatgat acatatccaa cgcactgaca ggctcatcta gcagcagaat
     9781 gtgtggttta cgtagcaata cttgtgcgaa cattaccatt tgccgttgcc cgccacttag
     9841 ccgcataata tcttgatgag ctagatgtga aataccgagt ttatccataa tatcaacggc
     9901 ttcattgagt aattcatcgc taatatacat attcaggcta tcaagttgcc ctaataaaat
     9961 aacctctaag gcactgagtg aggcctctac ctgtgtgtct tgtggcatat agccaatttc
    10021 tttacgccaa ctatgcaagt ggcttgaggt taatttatta ccgtcaaaat caattgtgcc
    10081 ttgatgagca atttccccaa atagagtttt aaggagagaa gatttacccg caccatttgg
    10141 gccaagtaaa gtatagatct tgccttgttc aaaatgtaga ttgattttat cagccaccac
    10201 tga
//
LOCUS       DANLXF010000078        10069 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.078, whole genome shotgun sequence.
ACCESSION   DANLXF010000078 DANLXF010000000
VERSION     DANLXF010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10069
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.078"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            342..1694
                     /gene="lysC"
                     /locus_tag="PXD66_001270"
     CDS             342..1694
                     /gene="lysC"
                     /locus_tag="PXD66_001270"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HDL6319574.1"
                     /translation="MSHLSVAKFGGTSVANFDAMTSSANIVIADANTRVVVLSASAGV
                     TNYLVELANGCEKEHRDEVIAAVRQIQYNIIEKLQHQDAVKAEIDELLERIETLAESA
                     SLATSAALSDELISHGEMMSTKIFTQLLIERGHPAVWVDVRDVVATNSHYGKAAPNDE
                     KTQQQSDNVIKPLIASGKIVITQGFIGRDDEGKTTTLGRGGSDYSAALLAEVLNANDV
                     LIWTDVPGIYTTDPRVVPNAQRIDTMAFNEAAEMATFGAKVLHPATLLPAVRSNIPVY
                     VGSSKAPEQGGTWVTRDPQPRPTFRAIALRRNQILLTLSSLSMLHAQGFLANVFAILA
                     KHKISVDVITTSEVSVALTLDKTGSASSGADMLSAELLDELNAYCNVQVESDLALVAI
                     IGNNLHAQSGVAKKLFHTLENYNIRLISYGASSNNVCTLVKNAEADDVVRSLHKGLFE
                     "
     gene            complement(1765..4011)
                     /gene="parC"
                     /locus_tag="PXD66_001271"
     CDS             complement(1765..4011)
                     /gene="parC"
                     /locus_tag="PXD66_001271"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDL6319575.1"
                     /translation="MTTEINYEGIEQMPIKRFTEDAYLNYSMYVIMDRALPFIGDGLK
                     PVQRRIIYAMSELGLNASAKYKKSARTVGDVLGKFHPHGDSACYEAMVLMAQPFSYRY
                     PLVDGQGNWGAPDDPKSFAAMRYTESKLSKIAEILLGELGQGTVDYQPNFDGSLEEPK
                     YLPARLPHILLNGTMGIAVGMATDIPPHNINELADASVMLLDNPKATLDDVLSVVQGP
                     DYPTEAEIITPKAEIARMYEQGRGSIKMRAVWKKEEGEIVISALPHQASPSKIIEQVA
                     TQMRNKKLPMVDDIRDESDHENPIRIVIVPRSNRIDFDALMDHLFATTDLEKSYRVNM
                     NMIGLDGKPAVKNLLTILNEWLSFRRTTVTRRLNYRLDKILNRLHILDGLMIAFLNID
                     EVIEIIRNEDEPKAELMARFNLTDVQAEAILNLRLRHLAKLEEHQLQAEKSELEKERD
                     ELQLILGSERRLNSLIKKEIQADAKAFASPRRSPLVERAESKAIAESDLTPTEDVTVI
                     LSEKGWVRCAKGHDIDVQALSHRAGDGYLAHARGRSNQPVVFLDSTGRAYALDPTSLP
                     SARSQGEPLTGKITLPEGAVVQQVLMASPETKVLMASDSGYGFICTFADLVSRNKAGK
                     AVISLTENAKVLPPQLLENDENLSLVAMSNVGRMLVFPVSELPQLSKGKGNKIINISA
                     AAAKSGDEYLARLLVIKPTNSLVFVSGKRKITLKPSDVDNYRGERARKGSQLVRGLST
                     NSTVEIVE"
     gene            4196..5233
                     /gene="pstS"
                     /locus_tag="PXD66_001272"
     CDS             4196..5233
                     /gene="pstS"
                     /locus_tag="PXD66_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005756281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDL6319576.1"
                     /translation="MAKRFKLLTVLGISFSLSVAANAQTITGAGASFPYPIYAKWAGL
                     YEKETGNKINYQSIGSGGGQQQIIAKTIDFGASDDPMKGETLAEHKLLQFPAIIGGTV
                     PVVNIPELKSGELKLSGKVLADIFLGKITQWNDPAIVALNQDIALPAKTILVVRRSDG
                     SGTTFGWTNYLSKVSAEWKEKVGEGKSVKWPVGQGGKGNEGVAAYVKQLKYSIGYVEY
                     AYAKQNALAWVSMQNADGKFVQPSRETFAAAAANADWSQTAGMGVILTNEAGEKAWPI
                     TAASFILIHQHSDKPEQTKGVFKFFDWAFKHGKDAAAELDYVPLPDNVVEKIQTQWKT
                     EVKDGQGNSLY"
     gene            5314..6273
                     /gene="pstC"
                     /locus_tag="PXD66_001273"
     CDS             5314..6273
                     /gene="pstC"
                     /locus_tag="PXD66_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDL6319577.1"
                     /translation="MHKHKIDNKCLNHPWVEGLFHHTTRFFALLVFALLVVVLVSLLL
                     GSWEALSRFGVSFLWENEWNPVNDRYGALVPIIGTLISAGIALVIAVPVSFGIATFLT
                     ELAPQWLKRPVGTAVEMLAAIPSIIYGMWGLFVFVPLFQEYVQPYLIDTFGQLPFIGM
                     LFSGAPFGIGLFSAGLVLAIMIIPFIASIMRDVFSVVPPLLKESAYGLGSTTWEVIWK
                     VVLPYTKTGVIGAIMLGLGRALGETMAVTFVIGNAFMLPDSVFSPSTSIASAIANEFN
                     EAGGLQKSALMSLGLILFLITSIVLAASRLLIARMGKQEGKRE"
     gene            6270..7118
                     /gene="pstA"
                     /locus_tag="PXD66_001274"
     CDS             6270..7118
                     /gene="pstA"
                     /locus_tag="PXD66_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005650601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDL6319578.1"
                     /translation="MKNYQNRRFYCRKIKNVLMLSLAYSAVLFGLFWLVWIIVTLLQK
                     GIPALSWELFTRPTPAPNETGGLRNAIVGSILIVSTATLIATPIGILAGTYLSEYGRY
                     TKLAKFTRFLNDVLLSAPSIIIGLFIYAIYVAQVKHYSGWAGSLALAVLVIPIVVRTT
                     DSMLNLVPNNLREAAAALGCPRWRVITMISYRAARSGIITGVLLAVARISGETAPLLF
                     TALSNQFASLDMNAPMANLPVVIYQYAASPFADWNTLAWAGAMLITLFVLSLNIITRL
                     YFSKTK"
     gene            7128..7898
                     /gene="pstB"
                     /locus_tag="PXD66_001275"
     CDS             7128..7898
                     /gene="pstB"
                     /locus_tag="PXD66_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HDL6319579.1"
                     /translation="MLPTDLETKLHINNLNFYYGDFHALKNINMRIAKNKVTAFIGPS
                     GCGKSTLLRTLNRMFELYPNQRATGEINLDGENLLTSDTDISIIRAKVGMVFQKPTPF
                     PMSIYDNVAFGIRLFEKLPKSELNDRVEWALTKAALWNEVKHKLNQNGDSLSGGQQQR
                     LCIARGIAVKPEVLLLDEPCSALDPISTMKIEELINELKHDYTVAIVTHNMQQAARCS
                     DYTAYMYLGELIEFGDTKQIFDKPKFRQTEDYIKGKMG"
     gene            7982..8668
                     /gene="phoB"
                     /locus_tag="PXD66_001276"
     CDS             7982..8668
                     /gene="phoB"
                     /locus_tag="PXD66_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006996031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HDL6319580.1"
                     /translation="MNNKILIVEDERAIREMLSLYLSKQYQVIEAEDYQSAINKLGEK
                     PELILLDWMMPGRSGIHLIKYLKKQPETEKIPIIMLTARSSEEDCITGLNAGADDYIT
                     KPFSPQVLLARIEAVWRRSYDPPSNLINIDGLILDQQAQRVLVAEQEVKLSGTEYKLL
                     HFLMTHPEKVYRREQLLDFIWGNDIYVEDRTVDSYIRRLRKSLEPYGFERYIQTVRGS
                     GYRFSNHFQD"
     gene            8671..9951
                     /gene="phoR"
                     /locus_tag="PXD66_001277"
     CDS             8671..9951
                     /gene="phoR"
                     /locus_tag="PXD66_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HDL6319581.1"
                     /translation="MKVKLSLKHFLLELLLAAAVAGVFNLFFPAYFFWFSFCLLVLLI
                     WHHHTESSLLQLLRPNEQSGRSKVTTMEHLSQSAAFYHERLRQDKIKTLRLLSKLNKH
                     IQYLPDAIIICQENGEISWCNNVAQELFEFYWDKKSNKSIFNVIFYPEFKSYFRQQRH
                     HRPLVLLTHNKRYIELNLNRYDSNSRLIVARDVTSFVRLMHSRQTFLANMNHELRTPL
                     TILQGYLEMLENEPHNMALQQKALNAMKEQSQRMTNLLRQLSLLVKIEHSNDSEHNEV
                     QMSALISAIQKNTEILSQNQQILFDIEPNLTVWGDQEQLQSAVSNLIYNAIRHAGEGV
                     TIQICCQRDNQEPNTAIFSVQDDGIGIDAHHITHLTERFYRVDESRSNKTGGSGLGLA
                     IVKHALEQHHSTLEIESEAGKGSRFSFKLPLIKK"
BASE COUNT         2846 a         2225 c         2092 g         2906 t
ORIGIN      
        1 gtatttctat gcacactttt caatactgtt gaaaaagagt aatgggcttg ccattacggg
       61 agaggtgcaa cactcaggca acttgatttt aggcaactag ggccgtttga gaattaagtg
      121 atgggagtga ttgccgaggt gtgaattcac tagactggaa ttaacatcgg ctgaataggt
      181 tgaatcctat cagctgtcat ttaataggtt atttcaccat attaaatgga gtgctctgat
      241 taattggttc aacaataaaa ccgtataatc tactcaaatt tcctttccga atatccccta
      301 acacaatatt tatctatttt tgttttaagg aaatttttat tatgtctcat ctttctgtgg
      361 ctaaatttgg tggtacaagt gttgcaaatt ttgatgcaat gacctcttct gccaatattg
      421 taattgctga tgcaaatact cgtgtggtgg tactttctgc ctctgctggc gtaaccaatt
      481 acttagtgga acttgctaac ggctgtgaaa aagaacaccg tgatgaagtc atcgcagcag
      541 tccgccaaat tcaatacaat attattgaaa aattacaaca tcaagatgcc gttaaagcgg
      601 aaatcgacga attattagaa cgtattgaaa cattagcaga atcagccagc cttgctactt
      661 cagctgcctt aagtgatgag ttaatcagcc atggggaaat gatgtccacc aaaatcttca
      721 cgcaattatt aattgaacgt ggacacccgg cagtatgggt agatgtgcgt gacgttgtgg
      781 caacaaacag ccattatgga aaagcagcac caaatgacga gaaaacccag caacaatcag
      841 acaatgtgat taagccatta atcgcatcag gcaaaattgt gattacacag ggctttattg
      901 gtcgtgatga tgaaggcaaa accactactt taggacgtgg cggttcagac tactctgctg
      961 cattgttagc agaagtatta aatgctaacg atgtactcat ttggacagac gtaccgggga
     1021 tttataccac cgatcctcgt gttgtaccga acgctcaacg cattgacaca atggcattta
     1081 acgaagcggc agaaatggca acatttggag cgaaagtatt acacccagca accttattac
     1141 cggcagtgcg tagcaatatc cctgtatatg ttggctcaag caaagcaccg gaacaaggcg
     1201 gcacttgggt aacccgtgat ccacaaccac gcccgacttt ccgtgcgatt gcattacgcc
     1261 gcaaccaaat tttactcacg ctttccagcc tttcaatgct acacgcacaa ggtttcttag
     1321 cgaatgtatt tgcgatttta gcgaaacaca aaatttcggt tgatgtgatt accacttccg
     1381 aagtcagcgt tgcattgacc ctagataaaa cgggctcagc ctcttcaggt gcagatatgt
     1441 tatcagcgga attactcgat gagcttaacg catattgcaa tgtgcaagta gaaagcgacc
     1501 tcgctttagt cgctattatc ggcaacaact tacacgctca gtccggtgtg gcgaaaaaat
     1561 tattccacac tcttgagaat tacaacattc gcctaatcag ctatggagca agcagcaata
     1621 atgtttgtac cttagtgaaa aatgcagaag cagacgatgt ggttcgttcg cttcacaaag
     1681 gcttatttga ataagcaata actccattgc aagcggttat ttggggcaaa attgttggat
     1741 ttacccccta accgcttctt ctctttactc aacaatctct accgttgaat tggtactaag
     1801 ccctctcact agctgtgagc cttttcttgc tcgttcgcca cggtagttat caacatcgct
     1861 cggttttaat gtgattttgc gtttaccgga cacaaagacc agcgagttgg ttggtttgat
     1921 gactaataaa cgggctaaat attcatcacc ggattttgcc gctgcggcgg aaatattgat
     1981 gattttattg cctttacctt tggaaagttg aggtaattca ctcaccggga aaaccaacat
     2041 tctgccaaca ttactcatcg ctacaagcga taaattttca tcattttcca ataattgcgg
     2101 cggcagtact tttgcatttt cggttaaaga aataaccgct ttgccggctt tattacgaga
     2161 aactaaatct gcaaaggtgc aaataaagcc gtaacctgaa tcggatgcca ttagcacttt
     2221 agtttctgga cttgccatta gcacttgctg cactactgca ccttccggta gggtgatttt
     2281 gcctgtaagc ggttcgcctt gcgagcgtgc cgaaggcagt gaagttggat cgagagcata
     2341 agctcgccct gtactgtcta aaaataccac aggctgatta cttctacccc ttgcgtgggc
     2401 aagataacca tctccggctc gatggcttag ggcttgaaca tcaatatcgt gtcctttcgc
     2461 acaacgtacc cagccttttt cagacaaaat cacagtcaca tcttcggttg gagtcaagtc
     2521 actttccgca atcgctttag actccgctcg ttcaaccaat ggtgagcggc gtgggctggc
     2581 aaatgctttg gcatcggctt gaatctcttt cttgatcagg ctgttcaaac ggcgttccga
     2641 gcctaaaatc agttgcaatt catcacgttc tttttccagt tcggattttt ccgcctgtaa
     2701 ttgatgctct tccaatttgg ctaagtggcg taaacgtaag ttcaggatcg cctctgcttg
     2761 cacatcggtc agattaaaac gagccattaa ctcggctttc ggctcatctt cattgcgaat
     2821 aatctcaatc acttcatcaa tattgagaaa tgcaatcatc aaaccgtcta aaatatgcaa
     2881 gcggtttaaa attttatcta atcggtaatt caggcggcga gtgacagtgg tacgtctaaa
     2941 actcagccac tcattgagaa tagtcagcaa attcttcact gccggcttgc cgtctaagcc
     3001 aatcatattc atattgactc ggtagctttt ctccaaatcg gtcgtcgcaa acaggtgatc
     3061 cattaacgca tcaaaatcaa tgcgattaga gcgtggcaca atcacaatgc gaatcgggtt
     3121 ctcgtggtca gattcatcac gaatatcgtc caccatcggt agttttttat tccgcatttg
     3181 ggttgctact tgctcaataa tttttgaagg agaggcttgg tgcggcaagg ctgaaatcac
     3241 aatttcaccc tcttcttttt tccacaccgc acgcatttta atagaaccac gcccctgctc
     3301 atacattctg gcaatttccg cttttggtgt gatgatttca gcctctgtcg ggtaatccgg
     3361 cccttgtacc acgcttagta catcatcaag cgttgctttc gggttatcca gcaacatcac
     3421 actggcatca gccaattcat taatattgtg tggtggaata tcggttgcca ttcccaccgc
     3481 aatccccatc gtgccattca acaaaatatg cggtaaacga gccggcagat atttcggttc
     3541 ttccaacgag ccatcaaaat ttggctgata atccaccgtt ccttgcccta actcgcccaa
     3601 taaaatttct gcaattttag acagtttcga ttcggtataa cgcattgcag cgaaggattt
     3661 cggatcgtcc ggagcccccc agttgccttg tccgtccact aacggatagc ggtaagagaa
     3721 cggttgagcc attagcacca ttgcttcata gcaggcacta tcaccgtgcg gatggaattt
     3781 acctaataca tcccccacgg tacgggctga ttttttatac tttgccgagg cgtttagccc
     3841 aagttccgac atcgcataaa taatacggcg ttgcaccggt ttcaagccat cgccaataaa
     3901 cggcaatgcc cggtccataa ttacatacat cgaataattg aggtaagcat cttcggtaaa
     3961 acgcttaatc ggcatctgtt caatgccttc ataattgatt tctgtggtca ttttttatcg
     4021 atatttatat taaaaaaacg agggctattt tctacgattt agcctgttta gtctagcctg
     4081 aaacacaagc ggtcgttttt tctgattttt ttaccattgt catcattttg tcatattctt
     4141 tttctacact aagcacgtga aattaactca cgctaacttt aaggagaaca atatgattgc
     4201 caaacgattt aaactactta ccgttttagg catttctttt tcactttctg tcgcagcaaa
     4261 tgctcaaacc atcacaggag ccggtgcttc tttcccttat cctatctacg ccaaatgggc
     4321 aggtttatat gaaaaagaga ccggtaataa aatcaattac caatctatcg gttccggtgg
     4381 cggtcagcaa caaattattg ccaaaacaat tgatttcggt gcttctgatg acccgatgaa
     4441 aggcgaaaca ttagcagaac acaaactttt gcaatttccc gccatcattg ggggaactgt
     4501 accggttgtc aatattcctg aacttaaatc gggcgaactg aaactgtccg gtaaagttct
     4561 tgccgatata ttcctaggca aaattacaca atggaacgat ccggcaattg tcgcacttaa
     4621 tcaagatatc gccctgccgg caaaaacaat tttagtcgta cgccgctcag acggttcagg
     4681 cacaactttc ggttggacaa actatctttc taaagtctct gccgaatgga aggagaaagt
     4741 cggcgaagga aaatcggtga aatggccggt cggacaaggc ggaaaaggaa atgaaggtgt
     4801 cgcagcttat gtgaaacagc tcaagtattc tattggttac gtagaatatg cctatgctaa
     4861 acaaaatgct ctggcttggg tttctatgca aaatgcagat ggtaaatttg ttcagccttc
     4921 ccgtgaaacg tttgctgctg ccgctgctaa cgccgattgg agccaaacag ccggtatggg
     4981 cgttatttta accaacgaag cgggagaaaa agcctggccg attaccgccg ccagttttat
     5041 cttgattcat caacatagcg acaaaccaga gcaaactaaa ggggtattta aattcttcga
     5101 ctgggcattt aagcacggta aagatgcggc tgccgaactg gattatgtgc ctctaccgga
     5161 taatgtagta gagaaaatcc aaactcaatg gaaaacggaa gttaaagatg gacaaggaaa
     5221 ttcgctctac taatgcaaaa cgaccgcttg tatggagctt aactaacaac aagcggtcgt
     5281 tttttcacaa cattttgcaa aagagttgtc actatgcata aacataaaat tgataacaaa
     5341 tgcttaaacc atccttgggt agaggggtta tttcatcaca caacccgttt ctttgctttg
     5401 ctggtttttg ccttgttggt ggtggtgctg gtttcattat tgcttggcag ttgggaagca
     5461 ttaagccgat tcggggtatc attcctatgg gaaaacgagt ggaaccctgt gaatgaccga
     5521 tacggtgcat tagtgccgat tatcggcaca ttgatttctg ccggcattgc attagtcatt
     5581 gccgttccgg tctcgttcgg cattgcgact ttcttaaccg aacttgcacc gcaatggcta
     5641 aaacgccctg tcggcacagc ggtagaaatg ctagcggcta ttccctctat tatttacggt
     5701 atgtgggggc tgtttgtatt tgtaccctta tttcaagaat atgtgcaacc gtatttgata
     5761 gatacatttg gtcagcttcc ctttatcggt atgcttttta gcggagctcc tttcgggatc
     5821 gggctattca gtgccggctt ggtattggcg attatgatta ttccctttat tgcctcaatt
     5881 atgcgtgatg ttttcagtgt tgtaccgcct ttactgaaag agtctgctta cggtttaggc
     5941 tcaaccactt gggaagttat ttggaaagtc gtgctacctt acaccaaaac cggagtaatc
     6001 ggtgcaatta tgctcgggtt aggtcgagca ttaggcgaaa caatggcagt tacctttgtg
     6061 atcggtaatg cctttatgct acctgattcg gtattttccc cttcaacctc catcgcttct
     6121 gctatcgcca atgaatttaa tgaagccggc ggattacaaa aatcagcgtt gatgagccta
     6181 gggctaattc tgttccttat tacttcgatt gttttagccg cttcacgttt attaatcgca
     6241 cgaatgggta aacaggaagg aaaacgagaa tgaaaaatta ccaaaaccga cgcttttatt
     6301 gccgaaaaat aaaaaatgtc ttgatgttaa gtcttgccta ttccgctgta ttatttggct
     6361 tattttggct ggtctggatt attgttactt tactccaaaa aggtatccct gcactgtctt
     6421 gggaactgtt tacccgcccg actcctgcac caaatgaaac aggaggatta cgtaatgcaa
     6481 ttgtcggctc tatactgatt gtcagtaccg caacactgat tgcaacacca atcggtattt
     6541 tagccggcac ttacttgagc gaatacgggc gttataccaa attggcaaaa ttcacccgtt
     6601 ttttgaatga tgtgttgctc tctgctcctt ctattattat cggcttattt atttatgcga
     6661 tttatgtcgc acaggttaaa cactattcag gctgggcagg ctcattagca ttggcggtat
     6721 tagtgattcc gattgttgtt cgaactaccg acagtatgct caatcttgtg cctaataatc
     6781 tgcgtgaagc agctgcggct ctcggctgcc cacgatggag ggtaattaca atgatctctt
     6841 atcgtgccgc ccgctccggc attattaccg gcgtgttgct cgctgttgcc cgtatttccg
     6901 gagaaaccgc accgctattg tttaccgcat tatccaacca gtttgcctct ttagatatga
     6961 atgcaccaat ggcaaacctc cctgtggtga tttatcaata cgcagccagc ccgttcgctg
     7021 actggaatac attggcttgg gcaggtgcaa tgcttattac tctgtttgtg ctttctctta
     7081 atatcatcac tcgtctgtat ttcagcaaaa caaaataagg actctatatg ttaccaacgg
     7141 atttagagac aaaattacat attaacaacc tcaattttta ttatggcgat ttccacgctc
     7201 tgaaaaatat caatatgcgt attgccaaaa ataaagtgac cgcatttatc ggtccttccg
     7261 gttgtggaaa atcaacatta ctgcgaaccc taaaccgaat gtttgagctc tacccgaatc
     7321 aacgtgcaac gggggaaatc aatcttgatg gagagaatct actcacctcc gatacggata
     7381 tttcgattat tcgagccaaa gtcggtatgg ttttccaaaa accgacccca ttcccgatgt
     7441 ccatttacga taatgttgct tttggtattc gtctgtttga aaaattgccg aaatccgagc
     7501 taaacgatcg tgtagagtgg gcattaacca aagcggcttt atggaatgag gtaaagcata
     7561 aattaaacca aaatggtgac agtctctccg gcgggcaaca gcaacggctc tgtattgcac
     7621 gaggtattgc agtgaaaccc gaggtgttac tgctggatga gccctgctcc gcacttgacc
     7681 cgatttcgac tatgaaaatc gaagaactta tcaacgaact caagcacgac tacacggtag
     7741 cgatagttac ccacaatatg cagcaagcag cccgctgttc tgactatacc gcttatatgt
     7801 atctaggcga gctgattgaa ttcggtgata ccaaacaaat tttcgacaaa ccaaaattcc
     7861 ggcagacgga agattacatt aaaggtaaaa tggggtaatc tgcaagcggt cggttttagt
     7921 aaaaaatttg caaatttctg cgataaatcg accgcttgtc acaccgcatc ggagaggaag
     7981 aatgaataac aaaattctga tcgttgaaga cgaacgtgct attcgtgaaa tgctaagtct
     8041 ctatctcagc aaacaatatc aggtaattga agcggaagac taccaaagtg ctatcaataa
     8101 actgggcgaa aaacccgagc ttattttact ggattggatg atgccggggc gttcgggtat
     8161 ccatttaatt aaatacctaa aaaaacagcc tgaaaccgaa aaaattccaa tcattatgct
     8221 aacagcccgc agtagcgaag aagactgtat taccggtctg aatgccggtg cagacgacta
     8281 cattaccaaa ccgttttcac cacaggtgtt gcttgcccgg attgaagcag tatggcggcg
     8341 aagctatgat ccgccaagca atctcatcaa tatagacgga ttgattcttg atcaacaggc
     8401 acaacgggtt ttggttgccg aacaggaagt gaaattaagc ggcacggaat ataaactgct
     8461 tcacttttta atgactcacc ctgaaaaagt gtatcgtcgt gaacagttgc ttgattttat
     8521 ttggggtaat gatatttatg ttgaggatcg cacggtagac tcttatattc ggcgattgcg
     8581 taagagtctt gaaccctatg gttttgagcg ttatattcaa accgtacgtg gctcgggata
     8641 ccgtttttcc aaccattttc aggactaata atgaaagtta agctctcact taaacacttt
     8701 ttattggaac ttttgctggc agcagccgtt gccggcgtat tcaatctgtt tttccctgct
     8761 tattttttct ggtttagttt ttgtttactg gttctactta tttggcatca ccacaccgaa
     8821 tcttctctcc ttcagttact cagacctaat gagcaaagcg gcaggtcaaa agtaacgaca
     8881 atggagcatt tatctcaaag tgccgcattc tatcacgaac gtttacgcca agataaaatc
     8941 aaaaccttac gtctgttatc aaaattaaat aagcatattc aatacttacc cgatgcgatc
     9001 attatttgcc aagaaaacgg ggagatttct tggtgcaata atgttgcaca agagctattt
     9061 gagttttatt gggacaaaaa atcaaataaa agcattttta atgtgatttt ttatcctgaa
     9121 tttaaaagct atttccgcca acaacgacac catcgcccat tggtactgct gactcataat
     9181 aaacgctata ttgaattgaa tctcaatcga tatgacagta actccagact tattgtcgca
     9241 cgagatgtaa cctcttttgt ccgccttatg cactcacgac aaaccttttt ggcaaatatg
     9301 aaccacgaac tgcgaacacc tcttaccata ttacaaggtt atttggaaat gctggaaaac
     9361 gagccgcata atatggcatt acagcaaaaa gcgctcaatg cgatgaaaga gcaaagtcaa
     9421 cgtatgacta acctgcttcg ccaactctca cttttggtta aaatcgagca ttccaatgat
     9481 tccgaacaca atgaggtcca gatgtcggcg ttaatctccg ccatacaaaa aaataccgag
     9541 attttaagcc aaaatcagca aatactgttt gatattgagc ctaatttaac ggtatgggga
     9601 gatcaagagc aactccaaag cgctgtatca aatttaattt acaatgcaat acggcacgcc
     9661 ggtgaaggag ttaccattca aatatgttgt caacgtgata atcaagagcc gaatactgcg
     9721 attttcagcg tgcaagatga cggtattggt atcgatgccc atcatattac ccatctgaca
     9781 gaacgtttct atcgtgtgga cgaatctcgt agcaacaaaa ccggcggtag cggtttaggt
     9841 ctggctattg tcaaacacgc tcttgaacag caccattcca cactggaaat tgaaagtgaa
     9901 gccggtaaag gaagcagatt tagttttaaa ctacctctaa taaaaaaata gcgatgcaat
     9961 caaactattt tcacaaggct tttcacaaac taatccccat tttacactga agttttgaat
    10021 ttctttataa ctgaggtgca agatcaaaat aataggcgat tttttataa
//
LOCUS       DANLXF010000079        10039 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.079, whole genome shotgun sequence.
ACCESSION   DANLXF010000079 DANLXF010000000
VERSION     DANLXF010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10039
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.079"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(399..1584)
                     /locus_tag="PXD66_001278"
                     /pseudo
     CDS             complement(399..1584)
                     /locus_tag="PXD66_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252650.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1743..2300)
                     /locus_tag="PXD66_001279"
     CDS             complement(1743..2300)
                     /locus_tag="PXD66_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HDL6319582.1"
                     /translation="MIKPITVNEALIGNALLNIKEGNIHPCLKMGFSEEQLKAINNLS
                     IDEIMDISNSVVSFAKVEINHETFWKLLAIAQTNTQQRQIIDRALLLGASIEMLHQYF
                     GLSTSEVSARRQLLGIEEKMGRKAAASDEESTHIWEIWQRYKQRMESLDSQEGLELLC
                     LIAEEGNMNLTVVWKLVSEWYSKHK"
     gene            complement(2293..3942)
                     /locus_tag="PXD66_001280"
     CDS             complement(2293..3942)
                     /locus_tag="PXD66_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HDL6319583.1"
                     /translation="MTKDEKRLAAIAKGLNVSPISNTAPSYQTATPAHYSSDEEYITV
                     TLDKLRPYEHNPRKTRNPNFEMIKESIRRRGLDHKPNITRRPGEDFYIIADGGNTRIQ
                     ALKELFTETKDPKFWSISCQYKPWQGDTADSVEAELNLLIGHLIENDTRADLSFIEKA
                     LGILQAKEYYEKKLNKSLSARELATELEGDGYIIHYTLANKMERCVLYLYPHIPEVLF
                     KGLGHTQIDKLLAIRNNAEEVWAAHQFDTDITFESLWSENLSKFNEATPFQAKEFQSE
                     LITAMVEALDGKVSFETLYLDIDLDEQKFKKIAAKQQEIDNLVEKTVDQINTRQEQQA
                     AKFEPVIKNENKSVATESAKNVIDDVIDDNESDNEQNNAITDLSQLDAPLLDSQDNQT
                     EDYLSQIAHNFGLTPGMSIQEQREQRAESNGLTFACEGRQPVEDIWLIHPARQYRSEA
                     YSLALDIAESVGIDHLVEHVVKQPVDYTYAMKPTDSELSPSVLTLYSFLSMLQTAKHK
                     DENNCVLDSNFLFELDDITLVKLFRLIRVSRHIHQQGGEND"
     gene            complement(3951..5315)
                     /gene="dnaB"
                     /locus_tag="PXD66_001281"
     CDS             complement(3951..5315)
                     /gene="dnaB"
                     /locus_tag="PXD66_001281"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDL6319584.1"
                     /translation="MSNPNKPTTHLYSAEAEAAVIGGLLLDNTLFDDVIGKINSADFY
                     FGVHQALFKGITDLIEVGKPADILTLDEYLKQKNLLKEIGGFAYLAEVSKNTPSAANV
                     GAYADVVLLHSKHRQLLKLGQFIVDQTQTVKTPEKLESVIDEVEKKMTEFSLSDNTKS
                     ATDLSDIFASMLSRMELSAKNGDPVTGTPTGIQGIDEATTGGQPGDLIVIGARPSMGK
                     TAFSQTIAYHTLEKFESAPVFYHSMEMPADQILQRFLAMRARVGLQNIRQADRLDDEE
                     WAKLTAAMGYILEHWKNRLVIDDEGSLTPQKLRTKVRQNARKYGKPSAIFIDYIQLMQ
                     TSQRYENRNLEIANISRSLKLLAKEMDCPVYALSQLNRSLEQRANKRPINSDLRESGS
                     LEQDADVILFIYRDEIYDEDSAFKGTAEIIIGKQRNGPLTSVMTNFRSEFALFENRAS
                     TASH"
     gene            complement(5327..6151)
                     /locus_tag="PXD66_001282"
     CDS             complement(5327..6151)
                     /locus_tag="PXD66_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL6319585.1"
                     /translation="MKPLQNGTNKPFTITIASTKGGSAKSTNAANIGAFCAEHGLRTL
                     LIDTDTQPTLSSYYELEYQAPGGTYEFLHFKDVDPTHIISRTAIPNLDLIQSNDPSNK
                     ISPMLRDSPDGALRFSLLLQKIEGYDVIIIDTRGTRDITVDMSVLAADVLFCPILPHI
                     LSAKEFIRGTIGMYQDLETFTAFGFTLPPLKAMINCVDHTKDVKLVSDYLHTLFQNEF
                     DENKTLLEFSVPDRVAYREAATFSKPVYRQSQAEYETIRQLCALLMPQFAQSHFSE"
     gene            complement(6245..6330)
                     /locus_tag="PXD66_001283"
     tRNA            complement(6245..6330)
                     /locus_tag="PXD66_001283"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6294..6296),aa:Leu,seq:caa)
     gene            complement(6642..8102)
                     /gene="xseA"
                     /locus_tag="PXD66_001284"
     CDS             complement(6642..8102)
                     /gene="xseA"
                     /locus_tag="PXD66_001284"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDL6319586.1"
                     /translation="MSNILTVTQLNYSVRHLLEMEMSHIWLTGEISNFSQPVSGHWYL
                     TLKDDKAQVRCAMFKMKNVRVNFRPQNGMQVLVRASVTLYEPRGDYQLIIESMQPAGE
                     GLLQQQFEQLKAKLSAQGLFAQEHKKPIPTFVKTVGIITSSSGAALQDILHILQRRDP
                     SLSVVIYPALVQGKEATQDIVNMIELANRRNECDVLIVGRGGGSLEDLWCFNEESVAY
                     AIYHSQIPIISAVGHETDVTIADFVADLRAPTPSAAAELVSRDQNELKRQLQHQFDKV
                     NLAFDRVWNHKFNRFQQLPLRLNAQHPSKQIQSQQQRFDYMEYRLEQAIYALVAAKKQ
                     VGKELNVRLIHQHPQKQIERQSQMLKQLELKLHWQMEQQLSKKQQKWKNITQRFERNP
                     LPYQLYQQQTMVAKMSQRLAYSIEKIMLKEQRHFQELCTKLDGLSPLKILTRGYSITQ
                     TKDGKTLTSTENIERGEAIITQLKTGKIISQVVDFL"
     gene            complement(8253..8329)
                     /locus_tag="PXD66_001285"
     tRNA            complement(8253..8329)
                     /locus_tag="PXD66_001285"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8293..8295),aa:Met,seq:cat)
     gene            8539..9645
                     /gene="mltA"
                     /locus_tag="PXD66_001286"
     CDS             8539..9645
                     /gene="mltA"
                     /locus_tag="PXD66_001286"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HDL6319587.1"
                     /translation="MNWKAYKKAIAAASVVAIATVLVACSSSGQKGKTTSYDAEKAKF
                     GAKYNGRHFMPYGTHFQAGRLGLNAAPVNQLDFLQQLSNVRAYSSLADSYAIAYGKIS
                     RWISSGGNINDLAKYGISTRLMRGEDGYQNVLMTGYYSPVIKARRTPQGQYQHPIYAM
                     PSSKRFSRAEIYAGALQGQGLELAYSNSMLDNFLLGVQGSGYVDFGDGRLNYFAYAGQ
                     NGFKYASVGRLLVEDGEIPKEKMSVQAIREWGQRNPSRVQGLLERNPSYVFFKNDPTG
                     QVKGSAGVPLVPLASVASDRSIVPSGSVLLVEMPLIDSKGNWTGKHEMRLMVALDVGG
                     AVKGHHFDLYQGIGDKAGHIAGLMKHYGRVWVLN"
     gene            9658..>10039
                     /locus_tag="PXD66_001287"
     CDS             9658..>10039
                     /locus_tag="PXD66_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319588.1"
                     /translation="MLKKLSAIFLLLPSAVLANNLQNITASTEPKYKISEIDVRILIR
                     QLNNIEQCIYPELAKPGYQQIYANWNLAENLTMQYFEYQLLKELLGEENQKLMQNDTL
                     STEYFHLLHSRLNHQKANVDPEKCD"
BASE COUNT         2802 a         2001 c         2008 g         3228 t
ORIGIN      
        1 tcactgtaga acccctctac actgattatt ttttaatcac tgtgtacccc ctacacactg
       61 attgtttttt gctcattgta gagtccctct acactgatta ttttttactc actgtagaac
      121 ccctctacac tgattgtttt ttaatcactg tgtaccccct acacactgat tgttttttgc
      181 tcattgtaga gtccctctac actgattatt ttttactcac tgtagaaccc ctctacactg
      241 attgtttttt aatcattgta aacagtgttt acagtgatta ttttttagcc tttgaagcgg
      301 gccgcttcac tgattatttt tcactcactg tgtaatccct acacgctgtc tgttttttgc
      361 tcgctgttaa gccccttaac agtgattgtt ttttaacctt attgtaaaag taattcagct
      421 ctaaacttct gcatacggct cgccatttgc tctctgtctg ctggagttaa taccggtttc
      481 tcagtaggct gctcagtcag tttttgtata gaatgagcag aattattgtt agtctgattt
      541 tgtgagggaa gattttttcc taattcaaaa tagtagggtt taaattcacc ctgcttcgcc
      601 cgctggataa gcgtatagag ataacctttc ggttttttca catcctgctt ttgaataatg
      661 cgttgttgtg cttccagcaa aatagcttgg cataaaccga gatcgaatcc tttcattgcc
      721 tgtgcagtga gtttttgttc aagtgaggtt agtgggattt cttgtggcca agtaatttcg
      781 ctaggttccc cagtacagta cttattaata tataaagtac tgtacatctg acttccttaa
      841 tggaagtaag tctaaaatca atgacttatc ttcattttta gccctaactt ccttattgga
      901 agtctggttt tttgaggatt ttttcctgac ttcctcatca aaatccctaa cttccatagt
      961 ggaagtcagg atattttgtt gaattttgct taattcttgt tctgtttgcg atggctgttt
     1021 atctttcacc agctgctgtt gctggtatgt ttgataacga ctttgcaaac agtctaaatg
     1081 agagacataa tgccatttgg ttttgtctgt cattagactt tcaacaatat gatttgccac
     1141 accattaata aggctatctt tatgtttggt gcatttatct aaaaaagaga gatagtcgct
     1201 atttaattga attgcatcta aaattggcac aggttcatcg tgcaggatat acacattacc
     1261 ttgtacataa cctcgctcat cacgcactgt ttcacataaa gtaagccagc gagtaagacg
     1321 gagtaaatag agtgtttgag tcacgacttt ttccgataat ttagcctccg cataaggtct
     1381 atcggataac attttgctga gcgtctcata agagggaaat aatcctcctt gaaaggcctg
     1441 aacattgcac ttaatgagct gccaagcaaa ctttgctctt ggtgtaaggc agggatcttg
     1501 taataatcga gtagggactg tttcgtgttg gttgccaaaa aatagtaatc catgccctgt
     1561 tggcttattc gtactgctaa acattgtttc tctccactag gataaaagag tttttctctt
     1621 gctattctcc ctcctatgac tataatgcac gggacttcgg caaagtcctt gtaattcata
     1681 ggatagatat acaagaccca atatcgaatg ccccaaagct ccaacttcgg ggcatttgtt
     1741 ttttacttat gttttgaata ccattcggaa actagtttcc aaacaacagt tagattcata
     1801 ttaccttcct cagcaatcag gcaaagaagc tctaaccctt cttgtgaatc taggctttcc
     1861 attcgttgtt tatatcgttg ccaaatctcc caaatatgag tggattcctc atcacttgct
     1921 gcggctttcc gtcccatctt ctcttcaatg ccgagtaatt gacggcgtgc tgaaacctct
     1981 gatgtcgata aaccgaaata ctggtgcagc atctcaatag aggctcccag taataaagcc
     2041 cggtctataa tttgtcgctg ttgagtatta gtttgtgcaa ttgccagtaa tttccaaaaa
     2101 gtctcgtggt taatctctac ttttgcaaaa gacactacgg agttgctaat atccataatt
     2161 tcatcaatac tgagattatt gattgctttt agttgctctt cagaaaatcc catttttaaa
     2221 caaggatgga tatttccttc tttaatattg agcagggcgt taccaatcaa tgcttcattt
     2281 acggtaattg gcttaatcat tttctcctcc ttgttgatga atatggcgac tgacccgaat
     2341 gagacggaaa agtttgacta aggtaatatc atcaagctca aacaggaagt tggaatctag
     2401 gacacagtta ttttcatcct tatgtttagc tgtctgtaac attgaaagga atgaatagag
     2461 tgttagaaca gacggtgaaa gttcactatc tgtcggtttc attgcataag tgtaatccac
     2521 aggctgtttc acaacatgtt caaccaagtg gtcaatgccc acactctctg caatatctaa
     2581 ggcaagggaa tacgcctctg aacgatattg acgagcagga tgaatcaacc aaatatcttc
     2641 aaccggctgg cgaccttcgc aagcgaaagt gagcccattg gattctgctc gttgctctcg
     2701 ctgttcttga atgctcattc ccggtgttag tccgaagtta tgagcaattt gggagagata
     2761 atcctcagtt tgattatctt ggctatctaa caatggagca tctagctgag ataagtcagt
     2821 aatggcgtta ttctgctcat tgtcactttc gttatcatct attacatcat caataacatt
     2881 tttagctgat tctgtggcaa cagatttatt ttcatttttg ataactggtt caaattttgc
     2941 tgcttgttgt tcctgtctgg tattgatttg atcgactgtt ttttcaacta aattatcaat
     3001 ttcttgttgt ttagccgcta tttttttgaa tttttgttca tctaagtcaa tatctagata
     3061 aagtgtttca aaactcactt ttccatctaa agcttcaacc attgccgtaa tcaattcact
     3121 ttggaattct tttgcttgaa atggtgtagc ttcattaaat ttggataaat tttctgacca
     3181 gagcgattca aaagtaatat cagtatcaaa ttgatgggct gcccatacct cttcggcatt
     3241 attacgaata gctaataact tatcgatttg agtatgccct aaacctttaa atagaacttc
     3301 tggaatatgg ggatatagat aaagtacaca tcgctccatt ttattagcca acgtatagtg
     3361 gataatatag ccatcaccct ctaactcagt agctaattcc cttgctgaaa gtgatttatt
     3421 caatttcttc tcgtaatatt ctttcgcctg taaaatacca agagcttttt caataaatga
     3481 caaatccgca cgggtatcat tttcgatcaa atgcccgata agtaaattga gttcggcttc
     3541 gacactgtcg gcagtatcac cttgccaagg cttatattgg caactgatag accagaattt
     3601 cggatctttg gtctcagtaa acaattcttt aagtgcctga atacgagtat taccgccgtc
     3661 agcgataatg taaaaatctt ctcccggacg gcgggtaata ttaggtttat gatctaaccc
     3721 acgacgccgg attgactctt tgatcatctc aaagttagga ttacgggttt ttctagggtt
     3781 atgttcatac ggccgtaact tatctaatgt caccgtaata tattcctcat cactactgta
     3841 atgagccggt gtcgcagtct gataggaggg tgccgtattt gaaataggtg aaacatttag
     3901 ccccttcgct atagctgcta atcttttttc atcttttgtc ataatctgtc ctaatgtgat
     3961 gctgtgcttg ctctattctc aaaaagtgca aactcacttc taaaattggt cattactgaa
     4021 gtcagcggtc cgtttcgttg tttaccgata ataatttcag cagtgccttt gaaggcggaa
     4081 tcttcatcat aaatttcatc tcgatagatg aataaaatga catcggcatc ttgctcaagt
     4141 gagccggatt ctcgtaaatc agagttgata gggcgtttat tggcccgctg ttccaatgag
     4201 cggttcagct gagataaagc ataaacaggg caatccatct ctttggctaa caacttaagt
     4261 gagcgagaaa tattcgcaat ctccaaattg cggttttcat agcgttgcga agtctgcata
     4321 agctgaatat agtcaataaa aatggctgac ggttttccat acttacgggc attttggcga
     4381 accttagttc ttaatttttg aggagtcaga ctaccttcat catcaataac taagcgattt
     4441 ttccagtgtt caaggatata ccccattgca gctgtcaatt ttgcccattc ctcatcgtcc
     4501 aagcggtctg cttgtcgaat attttgcaat ccgactcggg cacgcatagc aagaaaacgt
     4561 tgtaaaatct gatctgccgg catttccata ctgtgataga acacaggagc cgactcgaat
     4621 ttttcaagtg tatggtaggc aatagtttga gaaaatgcgg tttttcccat cgaaggacgg
     4681 gcaccaatca caattaaatc acccggttgc ccaccggtag tcgcttcatc gatgccttga
     4741 atacctgtcg gtgttccagt cacaggatca ccatttttag ccgaaagctc catacgagag
     4801 agcatactgg caaaaatatc acttaaatct gttgcagatt tggtattgtc agaaaggctg
     4861 aattcagtca tttttttctc aacttcatca ataactgatt caagtttttc cggtgttttc
     4921 acagtctgtg tctgatcgac aataaactgc cccagtttta aaagttgccg gtgtttactg
     4981 tgtaacagaa ccacatcagc ataagccccc acattggccg ccgaaggggt gttttttgaa
     5041 acttctgcaa gataggcaaa accgccgatt tccttcaaca gatttttctg ttttaggtat
     5101 tcatctaatg tgagaatatc tgcaggtttc cccacctcaa ttaaatccgt aatccccttg
     5161 aataaggctt gatgcacacc aaaataaaag tcagccgaat taatcttgcc aatgacatcg
     5221 tcaaagagag tgttatctaa taacaatccc ccgatgacag cggcttcagc ttctgctgaa
     5281 tagagatggg tggttggttt atttgggtta gacataaatc ccccaactac tcagaaaaat
     5341 gtgattgtgc aaactgtggc atcagtaatg cacaaagctg acgaatggtt tcatattcag
     5401 cttggctctg gcgatagaca ggttttgaaa aagttgccgc ttctcggtat gccacacgat
     5461 caggcacaga aaattccagt aatgttttgt tttcatcaaa ctcattttgg aagagagtat
     5521 gcaggtagtc ggaaaccaat ttgacatctt tggtatgatc aacacagtta atcattgctt
     5581 ttaacggtgg cagggtaaaa ccaaaggctg taaaagtttc tagatcttga tacataccaa
     5641 ttgtgccacg aataaattct ttagctgaca agatatgagg aagaatcggg cagaaaagaa
     5701 catcagcagc caataccgac atatctacag taatatctcg agtgccacgg gtatcaatga
     5761 taatgacatc atagccttca attttctgga gcagtaaact aaaacgtaaa gccccgtccg
     5821 gtgagtctct tagcattggg ctgattttat ttgacgggtc attagactgg atcaagtcaa
     5881 gatttgggat agccgttcta gaaataatgt gggtaggatc tacatcttta aaatgcagaa
     5941 attcataagt tccaccggga gcttgatatt ccaactcata ataagagctt aatgtgggtt
     6001 gagtatcggt atcaataaga agggttctta gaccgtgttc tgcacagaat gcaccaatgt
     6061 tagcggcatt cgtactcttg gcagagcctc ctttggtcga agcaatagta atggtaaaag
     6121 gtttgtttgt accgttttgt aatggtttca taatttactc tcttaacaaa gttgaagtta
     6181 aggagtattg gcattataaa ctgtatattt agagtgagta ttatttgaaa ggaacgcagt
     6241 tgaatggtgc ctgaggtcgg actcgaaccg acacggttat tcaccaatgg attttgaatc
     6301 catcatgtct accaatttca tcactcaggc tgagagtgtt ttttattcaa tcgccagtat
     6361 ttcctattag tctacgcaaa agattttgaa tccgctgcgt ctaccgattt cgccacactg
     6421 gcttaatttt tgcacgatta tagggaaata tgaccgcttg tgcaagcgta aaaatgcctt
     6481 ttgaaatcgg ttgatttaaa aatcagcact caattcatct ttacaaaaat agttatgcac
     6541 atcacctgtg gataactaag tgaacaaccc tgtttataaa tatttaacct actgatttta
     6601 ctataaataa ttttaaaaat tggcagcgtt caaaatttcc cctacaaaaa atccactact
     6661 tgacttatta tttttcctgt ttttagttgg gtgataatag cctcaccacg ttcaatattt
     6721 tctgttgaag taagcgtttt accgtcttta gtttgggtaa ttgagtagcc tctggtcaag
     6781 attttcaatg ggctaagtcc atcaagttta gtgcataact cttgaaaatg tcgctgctct
     6841 ttcagcatta ttttttcgat agaataagcc aagcgttgcg acatttttgc caccatcgtt
     6901 tgttgctgat aaagttgata aggtagcggg ttgcgttcaa accgttgcgt aatgtttttc
     6961 catttttgct gttttttgct gagttgctgc tccatttgcc aatggagttt cagctccaat
     7021 tgcttaagca tttgggattg gcgttcaatc tgcttttgcg gatgttggtg aattaaacgg
     7081 acatttagct ctttccccac ctgctttttg gctgcaacaa gtgcataaat tgcctgctcg
     7141 aggcggtatt ccatataatc aaaacgttgc tgttgcgatt gaatttgttt gcttggatgc
     7201 tgagcattca ggcgtaaggg gagttgttgg aaacggttaa atttatggtt ccaaactcgg
     7261 tcaaaggcta aattcacttt atcaaactga tgttgtaatt ggcgtttcaa ttcattttga
     7321 tctctgctga ctaattccgc agcagccgaa ggcgttggtg cgcgcaaatc tgcaacaaaa
     7381 tcagcaatgg tcacatcggt ttcgtgtccc accgcactga taatcgggat ttgtgaatga
     7441 taaatggcat aagcgacact ctcttcgttg aaacaccata aatcctctaa tgaaccgcca
     7501 cctcgcccga caattaatac atcacactca ttgcggcggt ttgccagttc aatcatattc
     7561 acaatatctt gcgttgcttc tttgccttgc acgagagccg ggtaaataac cactgataag
     7621 ctcggatctc gtcgttgcaa aatatgtaaa atatcttgta atgcggctcc gcttgaggag
     7681 gtaataatac caacggtttt aacaaaagta ggaattggct ttttatgctc ttgagcaaac
     7741 aagccttgag ctgaaagttt tgctttaagt tgctcaaatt gctgctgtaa caagccttcg
     7801 cccgccggtt gcatactctc aataatgagc tgatagtcac ctcgtggctc atagagtgta
     7861 acacttgccc gcaccagcac ctgcataccg ttttgtgggc gaaaattcac tcttacattt
     7921 ttcattttaa acatcgcaca acgcacttgt gctttgtcat cttttaaagt gagataccaa
     7981 tgccccgaaa ctggttggct gaaatttgag atttcgccgg ttagccaaat atggctcatt
     8041 tccatttcga gcaaatggcg gacggaatag ttgagttggg taacagttaa aatattgctc
     8101 ataataatca aaaaagcgta aatgtagtag aagtgtaacg agaaatgaaa gcgagaggaa
     8161 gcttttgcag tcaacaagcg gttatttttt gctgtttttt ggcaaataaa acgaaaaccc
     8221 tcgatgtgat tcactatcga gggtttggaa tgtggttgcg ggggccggat ttgaaccagc
     8281 gaccttcggg ttatgagccc gacgagctac caagctgctc caccccgcgt ctgatgaggc
     8341 tataatatat caatttgaca taatgacaag taaaatcata caaaattgct ctaagtgatg
     8401 taaattacaa cagatatagt atcaaaatac gcttattgtt gtaaaaacaa tcaaattata
     8461 ttctggtttt attttgtcgc tgtttttgct attcttctgt ttatttttca cattttatta
     8521 acgatataag gatattcgat gaattggaaa gcatataaaa aagctattgc ggcagctagc
     8581 gtggtagcga ttgcaacggt attagtggcg tgttcgtctt caggccaaaa aggcaaaaca
     8641 acctcttatg atgcggaaaa agctaaattt ggagcaaaat ataatggtcg tcactttatg
     8701 ccttatggca cacattttca agctggacgt ttaggattaa atgctgctcc tgttaatcaa
     8761 ctagactttt tacagcagct ttctaatgtc agagcctata gtagcttggc agattcttat
     8821 gctatcgctt acggaaaaat cagtcgttgg atctcatcgg gcggtaatat taatgattta
     8881 gcaaaatatg gtattagtac tcgtttaatg cgtggcgaag atggctacca gaatgtgtta
     8941 atgacagggt attactcgcc ggttatcaaa gcccgtagaa cgcctcaggg gcaataccaa
     9001 cacccgattt atgcgatgcc gtctagcaaa cgtttttcac gggcggaaat ttacgcagga
     9061 gcattgcaag gtcagggctt agaactggct tatagtaatt caatgctcga taacttctta
     9121 ttaggtgttc aaggtagcgg ttacgttgac tttggtgatg gtcgcttaaa ctattttgct
     9181 tatgcagggc aaaatggctt taaatatgcc agcgttgggc gtttattagt agaagatggc
     9241 gaaattccga aagagaaaat gtcagtgcag gctatccgtg aatgggggca acgtaaccct
     9301 agccgtgttc aaggtttgtt ggagcgtaat ccatcgtatg ttttctttaa aaacgatcct
     9361 accggtcagg taaaaggctc tgccggtgtg ccgttagtac cacttgcatc tgttgcatca
     9421 gatcgtagca ttgtgccatc aggtagcgtg ttgttggtcg aaatgccgtt gattgatagc
     9481 aaaggaaatt ggaccggcaa acacgaaatg cgtttaatgg tggctcttga tgttggcggt
     9541 gcagtgaaag gccatcactt cgatttatat caagggattg gcgataaagc aggccatatc
     9601 gcaggtttaa tgaaacatta cggccgtgtt tgggtattaa attaatacag ggtaactatg
     9661 ttaaaaaaac tatctgccat attcttactt ctaccgagcg ccgtgcttgc aaataatttg
     9721 caaaatataa ccgcttcaac tgagcctaaa tataaaatct cagaaataga tgtcagaatt
     9781 ttgatacgcc agctcaataa tattgagcaa tgtatctatc ctgagttagc taaacccggt
     9841 taccagcaaa tctacgcaaa ttggaatctg gctgaaaatt taacgatgca atattttgaa
     9901 tatcagcttc taaaagagtt gctcggagag gaaaatcaga aactgatgca aaatgatacg
     9961 ctttctaccg agtatttcca tttactgcat tctcgtttaa atcaccaaaa agcaaatgta
    10021 gatcccgaaa aatgcgata
//
LOCUS       DANLXF010000080         9905 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.080, whole genome shotgun sequence.
ACCESSION   DANLXF010000080 DANLXF010000000
VERSION     DANLXF010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9905
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.080"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            141..800
                     /locus_tag="PXD66_001288"
     CDS             141..800
                     /locus_tag="PXD66_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL6319589.1"
                     /translation="MFNISKQQVLDAFHFRSACRSYDPNKKINKEDMDYILELGRLSP
                     SSVGSEPWKFLVLQNPETRQKIAPVSWGIKHPMEEMSHLVVILTKKNVRYDSVYMRDM
                     LAKRGLTYEQIEASLVRYKSFQEEDMKLLSERELFDWSTKQTYIALGNMMTGAALIGI
                     DSCPIEGFHYDTVNQILSDEGLFDLNEYGVSCMITFGYRNKEIKKKSRKPTEEVIAWI
                     E"
     gene            complement(860..2185)
                     /locus_tag="PXD66_001289"
     CDS             complement(860..2185)
                     /locus_tag="PXD66_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HDL6319590.1"
                     /translation="MLIFNLIVFAAILFLLAKIYKSTQKLGQTVFIGLFLGLASGAFL
                     QTFYEKATVDATLDWINLIGNGYVRLLQMIVMPLVFVSILSAITRLKEAGSLGKISFS
                     VLSVLLVTTAIAAAIGIALVYLFDLSAEGLVAGSRELAAQEKVVGRVEQVSNLSVPSM
                     LLSFIPKNPFLELTGANPTSIINTVIFSALLGIAALSLGKEDKALGERIAQGVETLNK
                     LVMRLVRFVIRLTPYGVFALMIKMAATSQWSDIVNLGSFIVASYIAIGLMFIVHGILL
                     FVAKINPLDYYKKVLPTLSFAFTSRSSAATIPLNIETQTNKLGNNSVIANFSATFGAT
                     IGQNGCAGIYPAMLAVMVAPTVGMDPFSLNYILTLILVVAISSFGIAGVGGGATFAAI
                     VVLSTLNLPIELVGLLISVEPIIDMARTALNVNGAMVAGTLTNKWVKAE"
     gene            complement(2286..3038)
                     /locus_tag="PXD66_001290"
     CDS             complement(2286..3038)
                     /locus_tag="PXD66_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon-containing protein"
                     /protein_id="HDL6319591.1"
                     /translation="MYQAIVHFSYHNFEQDPVTAISQILNQWLYNGQVIGREMPITFH
                     QNEFQARVCVPTQESLLPQYNSDEVNEALQQAVEIGVIFASFELVGRDYQGEESSHNR
                     SPAFQILYTTHLDTCSPLYDGEQFAPIPLYSLQDQSLSEAILEWQQNWQACDLLQMNG
                     NLLECHALVQISDETSDLAMIGRDLCQQIEQKTHIPTFYYLYRLGRDESEYQRKCPCC
                     GGEWKLTEPLHDIFHFRCEPCRLISNLSWELL"
     gene            complement(3038..3943)
                     /locus_tag="PXD66_001291"
     CDS             complement(3038..3943)
                     /locus_tag="PXD66_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HDL6319592.1"
                     /translation="MNEKYSKLVKNAANLAIIVAILLIVIKAFAWWKTGSISILAAMT
                     DSVVDLFASLTNILVLRFALQPADENHAFGHGKAESLAALAQSAFISGSAVFLLLQGF
                     HKLTNPELIAQSSIGVIVSIVSIVITALLVFYQRYVVTLTKSPAIEADSLHYQTDLLM
                     NAAILVAMLLNSAGFVYADAVFAIGIALYIAFNAVKMFWCAVQTLLDQALPDEEIERI
                     KEIASQHPNILGIHDILTRRAGAVRFIQLHLELDDHLTLLVAHEITDSLEQKLLAAFP
                     MSHVIIHQEPTTVVKQEFQDMAENI"
     gene            complement(3952..4515)
                     /locus_tag="PXD66_001292"
     CDS             complement(3952..4515)
                     /locus_tag="PXD66_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATPase"
                     /protein_id="HDL6319593.1"
                     /translation="MSAIKKGITLVALSLAGCVSQSPQFAVPDAIHFGQDTFVKVTHS
                     QIDEMQHLLYLPEKSEKNPENWQKGILFFLDKNSKNQTLEQRAAFRQASFAKKPELEA
                     KVEIQQNELHSSVIYPPIERFNNVMLEVTRGRNLACGYGQIQFADKREVGKTVAKKSP
                     NLTAYSQEIARLSLELNQLGWQVQCSK"
     gene            complement(4515..5291)
                     /gene="yaaA"
                     /locus_tag="PXD66_001293"
     CDS             complement(4515..5291)
                     /gene="yaaA"
                     /locus_tag="PXD66_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HDL6319594.1"
                     /translation="MLAIISPAKTLDFKTNIDGFAFSQPNLTACSQELIDICKQLSPA
                     ELGSLMSISDKLAALNVARFAEWQLEHNEQNAKAALFAFKGDVYTGLEAETLSKAQVE
                     YAQQHLRMLSGLYGLLKPLDLMQPYRLEMGTKLANPKGKDLYAFWGNIITENLQQAID
                     EQGDDILVNLASDEYYGAVKPQNLNATIIKPVFLDEKNGKYKVISFYAKKARGMMVRF
                     ILDTQPTSIEQLKQFNYGGYWFDADSSSESELVFKREEQA"
     gene            complement(5480..7200)
                     /gene="ltrA"
                     /locus_tag="PXD66_001294"
                     /pseudo
     CDS             complement(5480..7200)
                     /gene="ltrA"
                     /locus_tag="PXD66_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012341372.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(8882..9277)
                     /gene="rpsI"
                     /locus_tag="PXD66_001295"
     CDS             complement(8882..9277)
                     /gene="rpsI"
                     /locus_tag="PXD66_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDL6319595.1"
                     /translation="MTAANQNYGTGRRKSSSARVFIKPGSGNITINQRSLEVYFGRET
                     ARMIVRQPLELVELTDKLDLYITVKGGGISGQAGAIRHGITRALMEYDETLRPALRAA
                     GFVTRDARRVERKKVGLHKARRRPQYSKR"
     gene            complement(9294..9722)
                     /gene="rplM"
                     /locus_tag="PXD66_001296"
     CDS             complement(9294..9722)
                     /gene="rplM"
                     /locus_tag="PXD66_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDL6319596.1"
                     /translation="MKTFVAKPETVQRDWYVVDATGKTLGRLATELARRLRGKHKPEY
                     TPHVDTGDYIIVINADKVAVTGKKQTDKIYYWHTGYVGGIKDATFKEMIARRPEAVIE
                     IAVKGMLPKGPLGREMFRKLKVYAGSEHNHQAQQPQVLDI"
BASE COUNT         2839 a         2206 c         1822 g         3038 t
ORIGIN      
        1 attgattttt aaggtaaagc aattttaaac aagcggtcat tttttcctaa ttttttgcaa
       61 attaacaggc gtgaaattcg cattctgctc taaatcaagt tatcattaat ctacttattt
      121 cacttataaa aggaaaaacg atgtttaata ttagcaaaca gcaagtttta gacgcttttc
      181 acttccgttc ggcctgtcgc agttacgatc caaacaaaaa aatcaacaaa gaagatatgg
      241 attatatttt ggaactcggg cgtctctcac caagctctgt cggttctgaa ccgtggaaat
      301 ttttagtgct acaaaacccg gaaacccgtc agaaaatcgc cccggtttcg tggggaatta
      361 aacacccaat ggaagaaatg agccatttag tggtgatttt aaccaaaaag aatgtccgtt
      421 atgattctgt ctatatgcgt gatatgttag ccaaacgagg cttaacctat gagcagattg
      481 aagcctcgct ggtacgctat aagtcgttcc aagaagagga tatgaaattg cttagtgaac
      541 gtgaattatt cgattggagt accaagcaaa cctatattgc attaggcaat atgatgaccg
      601 gtgcggctct gattggtatt gactcttgcc cgattgaagg ttttcattac gataccgtaa
      661 accaaatttt atcggacgaa ggcttatttg acctaaacga atatggtgtg tcttgtatga
      721 taacttttgg ctatcgcaac aaagaaatta agaaaaaatc ccgcaaacct accgaagaag
      781 tgattgcgtg gattgaataa tcaccttaac ttataaaaac gggcgaatta atcgcccatt
      841 tttatatctt tagttttgac tactcggctt tcacccattt attggttaat gtgcccgcca
      901 ccattgcacc attcacgttt aaggcggtgc gagccatatc aataatcggc tcaactgaaa
      961 tgagcaagcc tactaactca atcggcagat tcaaggttga tagcaccaca atcgccgcaa
     1021 aagttgcacc accacctaca ccggcaatgc caaatgagga aattgccacc accaaaatca
     1081 aggttaaaat atagtttaag ctaaacggat ccattcccac tgttggagcg accatcaccg
     1141 ccaacattgc cggataaatt cccgcacagc cattttgccc gatggttgcc ccaaaggttg
     1201 cagaaaagtt ggcaatcaca ctgttattac ctaatttatt ggtttgcgtc tcaatattca
     1261 acggaatggt tgccgcactg gaacgagacg taaaagcaaa acttaatgtc ggcaacactt
     1321 ttttgtagta atccaacggg ttgattttag ccacaaagag taaaataccg tgcacaataa
     1381 acatcaaacc aatcgcaatg tatgatgcca caataaagct acctaaattt acaatatcgc
     1441 tccattgaga agttgccgcc attttgatca tcaacgcaaa tacgccataa ggggttaggc
     1501 gaatcacaaa acgtactaaa cgcataacca gtttatttaa ggtctctaca ccttgtgcga
     1561 tacgttcgcc caacgcttta tcttctttgc ctaagctcag tgcagcaatg cctagcagag
     1621 ccgagaaaat aaccgtgttg atgatcgacg ttgggtttgc accggttaat tctaaaaacg
     1681 gatttttcgg aatgaaagaa agcaacattg aaggcacaga taagttgctc acctgctcca
     1741 cacgccctac cactttctct tgagcggcaa gctcacggga gccggcgact aaaccctcgg
     1801 cagataaatc aaacagatac actaaagcaa taccaatcgc tgcggcaatc gcggtggtca
     1861 ctaataacac tgatagcaca ctaaaactaa ttttgcctaa agatcctgcc tcttttaagc
     1921 gggtaattgc cgataaaata gagacaaaca ccaatggcat tacgatcatt tgtagcaagc
     1981 gaacataacc gttaccaatt aagttaatcc aatcaagagt tgcatcaaca gtagcttttt
     2041 cgtaaaaagt ttgcaaaaaa gcaccgcttg caagccctaa aaataaccca ataaacactg
     2101 tttgaccgag tttttgcgta gatttataaa ttttagccaa taaaaataat attgccgcaa
     2161 agacaattaa gttgaaaata agcacagaac ctccaaagta aaaccgctta ttataaaaat
     2221 gccgcaaggg tattacgctt gcggcattca ttcaagatga tttattactt tggtataact
     2281 aaaagttata ataattccca gcttagatta gagatcaaac ggcaaggctc acatctaaaa
     2341 tggaaaatat cgtgcaacgg ctcagttaat ttccattctc cgccacaaca agggcatttg
     2401 cgttgatatt cactttcatc acgccctaaa cggtagaggt aataaaaggt gggaatgtga
     2461 gttttctgct caatttgctg acatagatct cgaccgatca ttgcaagatc gcttgtttca
     2521 tcactaattt gaacaagtgc gtggcactcc agcagattgc cgttcatttg tagcagatcg
     2581 cacgcttgcc aattttgctg ccattccaat atcgcttcgc taagagactg atcctgtaaa
     2641 gaataaagcg gaattggggc aaactgctct ccatcataaa gtggcgagca ggtatctaaa
     2701 tgggtggtat ataatatttg aaacgctggc gagcggttat gactgctttc ttcgccctga
     2761 taatctcgcc cgacaagctc aaaacttgca aaaattacgc cgatttcgac cgcttgttgc
     2821 aacgcttcat ttacttcatc actattatat tgtggcaaaa ggctttcttg tgtaggcaca
     2881 caaacccgag cttgaaactc gttttgatga aaagtaatcg gcatttctct gccaatgact
     2941 tgcccgttat ataaccattg atttaagatt tggctaattg ccgtcaccgg atcttgctca
     3001 aaattatgat agctgaaatg cactatcgcc tgatacatta aatattctcc gccatatctt
     3061 gaaattcttg tttgaccacc gtagtcggct cttgatgaat aatcacgtga gacatcggaa
     3121 atgccgccag caatttctgc tcaaggctat cagttatttc gtgagctact agcagcgtta
     3181 agtgatcgtc taattccaaa tgtaattgga taaaacgcac tgcacctgca cggcgggtta
     3241 gaatatcgtg aatccctaaa atgttcggat gttgcgaagc aatctcttta atccgctcaa
     3301 tttcttcatc gggcaaagct tggtctagca aggtttgcac cgcacaccaa aacattttta
     3361 ctgcattgaa agcaatgtag agggcaatac ctattgcaaa aactgcatcg gcgtacacaa
     3421 aaccggcact attaagcaac attgccacta aaattgccgc attcattaat aaatcagttt
     3481 ggtaatgtaa cgaatccgct tcaatcgccg gacttttcgt aagcgtgaca acataacgct
     3541 gataaaagac taaaagtgca gtaatcacaa ttgatacaat actgacaatt accccaatac
     3601 tcgattgagc aattaattcc ggattggtga gtttatggaa accttgtagc agtaggaaaa
     3661 ccgccgagcc ggaaataaac gcactttggg caagtgccgc caaagactcc gctttgccgt
     3721 gcccaaacgc gtgattctca tctgccggtt gtagtgcaaa acgcagcact aaaatattgg
     3781 ttagcgaagc aaacagatcc actaccgaat ccgtcattgc tgccaagatt gaaatcgaac
     3841 cggttttcca ccatgcaaag gctttaatca caatcagtaa gatcgcaaca ataatcgcta
     3901 aattcgctgc atttttgact aatttgctgt atttttcgtt catttactac attatttact
     3961 gcattggact tgccaaccca gttggtttaa ttcaagagaa agtctagcaa tttcttggct
     4021 gtaagcggtc aaattcggcg atttttttgc aactgtcttg ccaacttccc gcttatctgc
     4081 aaattgaatc tgcccgtaac cacaagctaa attccgccca cgggttactt ctaacatcac
     4141 attgttaaaa cgttctatag gtggataaat cacactgctg tgtaattcat tttgctgaat
     4201 ttccactttc gcctcaagct ccggcttttt cgcaaaactt gcttggcgga atgcagctcg
     4261 ttgctctaag gtttgatttt tactgttttt atctaagaaa aataaaatgc ctttttgcca
     4321 attttccgga tttttctcac tcttttctgg caaataaagt aaatgttgca tttcatcaat
     4381 ttggctgtgg gtgactttta caaaagtatc ctgcccaaag tgaatagcat caggcactgc
     4441 aaattgtggg ctttggctga cacaacctgc caatgataac gcaaccaaag ttattccctt
     4501 tttgatagcc gacattatgc ctgctcctca cgtttaaaga ctagctcgct ttcactagaa
     4561 ctgtctgcat caaaccaata gcccccgtag ttaaactgtt tgagctgttc gatgctagtc
     4621 ggttgggtgt ctaatataaa gcgaaccatc attccacggg cttttttggc atagaagctg
     4681 atcactttat atttaccgtt tttctcatct aaaaacaccg gcttgataat agtcgcattt
     4741 agattttgtg gcttaaccgc accataatat tcatcagagg caagattaac taaaatatca
     4801 tcgccttgtt catcaatcgc ttgctgcaaa ttttcagtaa taatattgcc ccagaaagca
     4861 taaagatctt tgcctttcgg attggctaac ttagtgccca tttctaaacg ataaggctgc
     4921 attaaatcta acggctttaa taaaccatac aagcctgaaa gcatacgcaa atgttgttga
     4981 gcatattcaa cttgtgcttt gcttaacgtt tctgcctcta gtccggtata aacatcacct
     5041 ttaaaggcaa agagggcggc tttggcattt tgttcgttat gctcaagctg ccactcggca
     5101 aagcgagcca catttaaagc ggctaattta tcgctaattg acatcaaact acctaattca
     5161 gcaggtgaaa gctgtttaca aatatcaatt aattcttggc tacaagcggt caaatttggt
     5221 tgagaaaatg caaaaccgtc aatattggtt ttaaaatcaa gggttttggc aggggaaata
     5281 atggctaaca taaatgcctc tatcaaatag ttaatcaggg cggttattct accacagtca
     5341 taaagacaaa aaaggcagga aaccctgcct tcttgaaaac gaaaagaaaa gtcgggtagc
     5401 gaaagtacat tactgcacct ccgcccccta agaactgtac aagcaaattt ctctgcatac
     5461 agctcaagcc tttcatcagc tatatagccg gcttcacgac ctccaagttc aaagtatgaa
     5521 tctttatgtg acaagtcgga tgtaccaaac aacgattgct taatgcatcg cttcctccta
     5581 acattttgta cacgatatga tgatcgtgcc agcctgtttc tttggttatt ggcaacccac
     5641 ataatgcaca ttttccacgc tggtctttat acagtgcctg ccattgtcgt ctgtgtgatt
     5701 gttcttcata cagacgttct tggctcagtt tttcgccgta caactcccat tcaggcaaaa
     5761 acggattata ttcagactta attttaacgt gtcgtttgat tttaacatag ccacaataga
     5821 ataagtctat cggcttagtt tctccttttt cattggtata aactgtgcca aatacccaat
     5881 ttcgagtcgc tgtggtacaa aagtatttct cttttatcca cataatacct ttattgtgat
     5941 gacggcgttc acaccatttc caaagtactt tccaaactaa tgcgtccatt cgtccgaatg
     6001 ccttacttgc aacttggctt cgatgataat ttgcccaacc tcgtaacatt gggtttagca
     6061 ccattataag atcttcctgt ttagccattt tcatttagaa attacctctt ttaccttgtt
     6121 gtagaacgct ttcacattct tttgacttgg tctgttcagt atttttcctt tgaaacgtct
     6181 cacattccaa cctagaaaat caaaaccttc ttcaatatgt accactttcg tcttttctgt
     6241 tgataaggat aaacctcgtt cattcagaaa gttctggata agtgggataa cttgattttc
     6301 aagtaactct ttcgttttac ctgatatgat aaagtcatct gcataacgta ctaagtaagt
     6361 tttattttct cgaattgtcc gactgccttt tcctccaaaa tgagtttcta acagtttttc
     6421 caatccatct aatgccatat ttgcaagcgt tggcgagata atcccaccct gtggtgtacc
     6481 ttcctctgtt cgtttcaacc gtccaaattc cactacacct gattttagcc atttccgcaa
     6541 tatccgttta ttcattggaa tatgctttaa tagccagtcg tggcttatgt ggtcaaaaca
     6601 acctgcaata tccgcatcaa gtacccactc aacaggttga ttagttttct tgcctttatg
     6661 cgaataaatt tagtgtatat gagtaatagc atctaccgtt gaacggttta atctaaatcc
     6721 ataagaacct ttatccgcag ttgtttctgc cacaggttgt attgccaata aataaagggc
     6781 ttgcattgct ctatctcgca ttgttggaat acccaacgga cgtttctttc cattcggttt
     6841 ggggatatac actcgtctta atggactagg tttataccct ttttccgtca atgatagtgc
     6901 ggcttgccat tttgtctctg gggtctccca aagctcttta tcgacaccac ttgttcgctt
     6961 accttgattt tcagtcactc ttcttactgc aactaattta gcataatgtg agtgggttag
     7021 cattcgttgt aagtttttaa ccttacgcca attgccttcc agtgttgcct tcgcaatccg
     7081 aatttgcatt cctttcacat acgtttcagc tttatgccaa tcgacataat gccatttgga
     7141 gatgtgcgag gtcgctggat tgcaagctaa cttttcagta taatgcttac gctctttcat
     7201 acctacctct gatattacaa gataagtcga aagtctcagc aagtgggtag atccttcaag
     7261 taaacaaatc cctattgaga taaactatcc ttagtttatt ccctttggtt acaagaggat
     7321 cttaccgttt taccgataat ttcaaccgct cttgtaaccg attgggttaa agagtcgagc
     7381 gctaggttat gatgagccta acgctcattt ctttaatttg tttttgtacc tattcaacat
     7441 tgtattcctt gataacattc aatttctctt ctgttatcta caatctcttt cggtgttacc
     7501 cttaaaggaa acgctattca tcttacgatg aaagaccagt tagaagtctg caccctttcg
     7561 ggttaggcta tgtttcacac cctatctatg ccattacagc atagcattcg ctttttctaa
     7621 cctcccttac ctgcattccc aacagtatcg cttacgcttt acttgccttt tcctctttta
     7681 ccttcgccat ctatttcaaa cggtttgagt attgtgggca aggcagaaat acaggcttac
     7741 caagttccat tactatcaca cgagtagtga agatacagtc tctcccccga cagtgctttg
     7801 atggcgtatc ctcacgttaa agaagaataa ccgactgcct accttttggt cagagcctat
     7861 caatagcttt ggctctctct cgataacgag gtttatcaac tgttcactta cgtttatctt
     7921 tctacccagt ctagcccctt gaaggtgtgc tattcacttt ctcccaatgt cctctcacga
     7981 tttcattgac tgattttcat caggggttca ttgtcaggaa gctccgcacc acaccgttac
     8041 cggtgtcgca ctatccctag actactgtta gccatataac aggttacctt atcattccct
     8101 ccgtactact catatcttcg gattgtttga ccgtaacaat gacagtaata gcttacgcat
     8161 acttactaag tcagtatcct atactgcata aataagtccg aacctttaca aaataaattc
     8221 acttatgcga ctaaagtcgc tttgtctata tccaaatttt cccgcaaata gcctataacc
     8281 tgtccaatag gcgaaggtag ctaaccctag ctaatgtgag tattagaggt gattttaacg
     8341 agaatttaag gtataattat ccctagctca taaaaacgaa taaacaagag tttatcctta
     8401 gtgtttttat tcactattga ggagcattct acgattaccg tagattgcga gtgtgaaaag
     8461 tataagtaaa gatggcactc cttccctaag atttaccaag tcttagttct gatgtctttt
     8521 ctgagtgctg aggcgatcta ttggacttta tttatagtag gtgttgtaaa ttttctctgc
     8581 tctggggtat gggtaactcc ggtctgcgga tcttgctttc ttactctatc ttggtattaa
     8641 gaaaaaaacc aagtacatca aagtgaagat cataccttac agcctttcga gttggttcat
     8701 ttactttccc aacttagaac aagagtgctt gggcaaagcc ccttttctct tgcctgtctt
     8761 tccaagcact ccgaagagtg cttggaaagt cggaagcagg tgataagaat accacccact
     8821 tccagcaata actccttgat atataaggag ttattgccat aagtacgaca acttgtcgca
     8881 cttaacgttt tgagtattgt ggacgacgac gtgctttgtg taaacccact tttttacgtt
     8941 caacgcgacg tgcgtcacga gtaacgaagc ctgctgcacg aagtgctggg cgtagcgttt
     9001 catcgtattc cattaatgca cgagtgatac cgtgacggat tgcacccgct tgaccagaaa
     9061 taccaccacc tttaacagtg atgtaaaggt ctaatttatc agttaattca accaattcta
     9121 acggctgacg tacgatcata cgagctgttt cacgaccgaa gtaaacttca agtgaacgtt
     9181 ggttgatagt aatgttacct gaacccggtt tgataaatac acgggctgaa gagcttttgc
     9241 ggcgacctgt gccgtagttt tgatttgctg ctgtcatttt tatgctccgt gaattagata
     9301 tctaaaactt gtggttgttg tgcttggtga ttgtgttctg aacctgcgta aacttttaat
     9361 ttacggaaca tttcacgacc aagtggacct tttggtaaca tacctttcac tgcgatttca
     9421 atcacagctt caggacgacg agcaatcatt tctttgaagg ttgcatcttt gatgccacct
     9481 acatagccag tgtgccagta gtagattttg tcagtttgtt ttttacctgt tactgctact
     9541 ttgtctgcat tgatcacgat gatgtaatca ccggtatcaa cgtgtggagt gtactcaggt
     9601 ttatgtttac cacgaagacg gcgtgccaat tcagtcgcta aacgacctaa tgttttaccc
     9661 gtcgcatcta ctacatacca gtcacgttgt actgtttctg gttttgctac aaaagttttc
     9721 attaattaaa taccaaattt aaagtttaaa acccaatacc tcattgaggt atttcctatt
     9781 attttacggt gaacccgacc ccttcgagtc tgattcagac caaacttgtg tggcggggaa
     9841 aaccacacaa aaacagggtg gcgaaattat acagagattt cactcaaaaa tctaacattt
     9901 tttgc
//
LOCUS       DANLXF010000081         9894 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.081, whole genome shotgun sequence.
ACCESSION   DANLXF010000081 DANLXF010000000
VERSION     DANLXF010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9894
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.081"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(33..722)
                     /locus_tag="PXD66_001297"
     CDS             complement(33..722)
                     /locus_tag="PXD66_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005577085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HDL6319597.1"
                     /translation="MFMYLSHPLDPKDLAWPGEPVVEVTRCTDVGEETPFSSFITKLP
                     NHCGTHMDAPRHFVKDGLSINELPIEYFCHKEVALIEIPKGEAEGITKEDLLPYVDIL
                     SKVSFAFLRTGFEKYRTENPAIYQNECPYISTGAGQYLSDTFPNLKGVGIDFLALGSP
                     SSRVPDAENPKNCHRAILGYYTGRFTTVIEDMHLAELPKNANIKQFFNAPWRIKGLDS
                     SQVTCIVELQQ"
     gene            complement(734..2074)
                     /locus_tag="PXD66_001298"
     CDS             complement(734..2074)
                     /locus_tag="PXD66_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HDL6319598.1"
                     /translation="MLKKTLLFTLLLSHSLYAEDMIQENKSANITLLAEITAQGQKIN
                     GVALEYPDNVLSGSDLRQLYQVKTALDSAEKENRSVLKAYVNNEPKVTALPQAGKFVI
                     IELDTADKNAATYSVKDANKAPMKFKSKDENGNIVETEKVQAVKIPEFFANRLIYSAE
                     QKGLLKLTSGTTLGKTEWTQTAEPDKIKTPYLDDFVAKQVSLDQSGNKLNYRLYSPLQ
                     KNGEKYPLVIFLHGSGQVGSDNLAHLLSSKGAIATLPYAPSFVLAPQYDQVFDPFDNA
                     AKGQKGGIHWQTENRINLVLKMIDETLKANPQIDTTRIYINGLSRGAEGGLNLLLARP
                     NFFAGALLMSGREAYTNEWVDGNATKTNLAAIKHIPIWFFHSKEDKVSPVKGSRINYR
                     ILKDELNAPNVKYTEFTFEQAGDNGIVNNNPHNTWDAVYNSPEVIGWLLQQKRH"
     gene            complement(2136..3494)
                     /locus_tag="PXD66_001299"
     CDS             complement(2136..3494)
                     /locus_tag="PXD66_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntT protein"
                     /protein_id="HDL6319599.1"
                     /translation="MSNEMLILIGLASVVALLVIMIKGKVHPFLALGLVSIAVALTAG
                     IPMSKVIPTLISGMGSTLGSVALIVGLGAMLGKIIEKSHGADVLANWLLQRFGEKNAP
                     FALGMTGFIFGIPVFVDVGFIVLLPIIFSVARRLGGNILVYSLPIGLAMLLVHVLVPP
                     HPGIVAGAQNLNADIGFVLGIGLVAALPAYLIGQKFIPFFTKNNFVKVVTSTDLLQHQ
                     KDLIKEKGIELPSFGKVLAMISFPLILIMLGTVTATTLPKEHIVREVFSMVGSSPFAL
                     FVAVCLSAYVLGFRYGWTKHKLEEVLESALAPIAGIILITGAGGMFGKVLDASGVGKA
                     LADVLANTGLPLLVLSFILSALLRAAQGSATVATITTTTILAPAAVAAGYNDLQLALL
                     TAAIGAGSMTLSHVNDSLFWVWTKFFNVPVSVGLRTWTFLTTIFGVIAFAVVGIIWML
                     FV"
     gene            complement(3547..4323)
                     /locus_tag="PXD66_001300"
     CDS             complement(3547..4323)
                     /locus_tag="PXD66_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005547735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase family protein"
                     /protein_id="HDL6319600.1"
                     /translation="MPKFAANLTMMFNEVPFLDRFEAAANAGFKYVEYLWPYDYPAAE
                     LKAKLEQYGLKQVLFNTAAGDVAQGEWGVSAIPGREADSHRDIDLALEYALALGCENV
                     HVMAAVVPEGEDREAYKQTFIKNVQYAADKFKPHGINVLLEALSPEVKPNYLLKSQFD
                     TLEIVEAVNRDNVFVQLDYFHAQNVDGNLSRLTDKLKGKFAHIQIAPVPDRHEPDEGE
                     INYQYIFNKLDEIGYEGYVGCEYKPRAETTSGLGWFEQYK"
     gene            complement(4325..4957)
                     /locus_tag="PXD66_001301"
     CDS             complement(4325..4957)
                     /locus_tag="PXD66_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014668373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDL6319601.1"
                     /translation="MTEQEQKIEMVNLARSLFERGYTVGGAGNLSVRLDENRILVTPT
                     GSSLGRLEADRLSVLDMQGNVLSGDKPSKESVFHLAMYQKNPACQAIVHLHSTYLTAL
                     SCLENLDTDNAMRAFTPYYVMRVGKLPVIPYYRPGDINIARELGERALDGKAFLLANH
                     GVVVTGSDLRDAVDNTEELEETSKLYFILQGQKVRYLTDDEVNDLKNRGK"
     gene            complement(4954..6195)
                     /locus_tag="PXD66_001302"
     CDS             complement(4954..6195)
                     /locus_tag="PXD66_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005754893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HDL6319602.1"
                     /translation="MLGIIADDFTGASDIASFLTENGLKTVQMNGVPKTTLNSQVDAI
                     VISLKSRSNPVEEAIQQSLQALDWLKQNGCSQFYFKYCSTFDSTEKGNIGPVTDALMA
                     ALDTDFTVVTPALPVNGRTIFNGYLFVGDVLLNESGMRNHPITPMKDANLVRLMDAQS
                     QGKTGLVAYADVIQGAERVKARFAELKQQGYKYAVVDAADNSQLAVLAEAVADFQLVT
                     GGSGLGAYMAAYRSGGKKGTNAFVPSKGKTVVLSGSCSVMTNTQVAAYQQKAPSYFLE
                     AEKAIQDPNYVETLYQWVIANLDGDFAPMVYATVPPEKLKEIQTQFGGDKASHAIENT
                     FAKLAEKLQAAGVTNFITAGGETSSIVVQQLGFSGFHIGKQIAPGVPWLQAVEKPIFL
                     ALKSGNFGKEDFFSFAQEMFV"
     gene            complement(6197..7102)
                     /locus_tag="PXD66_001303"
     CDS             complement(6197..7102)
                     /locus_tag="PXD66_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDL6319603.1"
                     /translation="MSQKYSVAVIGLGAMGMGAAKSCIAAGLDTYGVDLNPNYLETLK
                     AAGAKKVGTSAVDFAAELDAVLLLVVNANQVNAVLFGENGLAQHLKQGTVVMVSSTIS
                     AQDAKNISQKLTDLGLVMLDAPVSGGAAKAAQGEMTVMASGSSEAFEKLKPVLDAVAG
                     KVYNIGEEVGLGATVKIIHQLLAGVHIAAGAEAMALAARAGIPLDLMYDVVTNAAGNS
                     WMFENRMKHVVEGDYTPLSMVDIFVKDLGLVNDTAKSLRFPLHLASTAYSMFTEASNA
                     GYGKEDDSAVIKIFSGIELPKKEGK"
     gene            7276..8034
                     /locus_tag="PXD66_001304"
     CDS             7276..8034
                     /locus_tag="PXD66_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL6319604.1"
                     /translation="MIPLERQREILRLINNNGVASINFLAEQLKVSHMTIRRDIQKLE
                     EQGKVSLVSGGVQSLERLIMELPHDDKSMLFQEQKSAIGSLASQLIPSNTTIYLDAGT
                     TTLEIAHRIIDRNDLLVVTNDFVIAHYLSNSGKCDLIHIGGSVCKENLSTTGSLAAEF
                     LKNLSIDMAFISTSSWNLKGLTTPNENKIPVKKAILEASKQNILVTDSSKYGKVATFF
                     VCPLTVFDQIICDKGLFENVRDALKGLSIKLDLG"
     gene            complement(8174..9565)
                     /gene="ffh"
                     /locus_tag="PXD66_001305"
     CDS             complement(8174..9565)
                     /gene="ffh"
                     /locus_tag="PXD66_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDL6319605.1"
                     /translation="MFENLSDRLSKTLRNITGKGRLTEDNIKDTLREVRMALLEADVA
                     LPVVRDFINKVKERALGVEVNKSLTPGQEFLKIVQSELETAMGEANEELNLAAQPPAV
                     ILMAGLQGAGKTTSVGKLAKFLKERHKKKVLVVSADVYRPAAIKQLQTLAEALKVDFF
                     PTETSQKPVAIAEAALKHAKLNFFDVLIVDTAGRLHVDGEMMEEIQQIHAVLNPIETL
                     FTVDAMTGQDAANTAKAFNEALPLTGVILTKVDGDARGGAALSIRQITGKPIKFLGVG
                     EKTDALEPFHPDRVASRILGMGDVMSLIEDIQRSVDQEKAEKIAAKFKKGDEFTLEDF
                     REQLIEMKKMGGMMSMLDKLPGAKNLPDHVKSQVDDKMFMKMEAIINSMTLKERANPD
                     IIKGSRRRRIALGSGTQVQDVNKLLKQFDDMQRMMKKMRKGGMAKMMRGVKGMMGGGL
                     GGLGGLGSMFGKR"
BASE COUNT         2819 a         2217 c         1985 g         2873 t
ORIGIN      
        1 atagcctctc ctaaaatgaa tatatctgtg gtttattgct gtaattccac aatacaagtc
       61 acttgactgg aatctaaccc tttaattcgc cacggtgcat taaaaaactg cttaatgttg
      121 gcatttttag ggagttccgc taagtgcata tcttcaatca cagtggtaaa cctgccggta
      181 tagtagccta aaatcgctcg atggcaattt ttagggtttt ccgcatcagg cactcgggaa
      241 gaaggagacc ctagtgccaa gaaatcaatg cctactcctt tcaaattcgg gaaagtatca
      301 gataaatact gcccggcacc ggttgaaata taggggcatt cattttgata aatagcaggg
      361 ttctcggttc tatatttctc aaaccccgtt cgtaaaaagg caaaagacac cttggagaga
      421 atatcgacat aaggcaataa atcttctttg gtgatccctt ctgcctctcc tttgggaatt
      481 tcaattaaag cgacctcttt atggcagaaa tactctatcg gcaattcatt gatacttaaa
      541 ccgtctttca caaagtggcg aggagcgtcc atatgtgtgc cgcaatgatt cggtagtttc
      601 gtgataaaac tgctaaatgg cgtttcttca cctacgtctg tgcagcgagt cacctcaaca
      661 acaggttctc ccggccaagc taaatcttta ggatctaacg ggtgagacaa atacataaac
      721 acagtgagcc tccttagtgg cgtttttgct gaagtaacca cccaatcacc tcagggctgt
      781 tatacacagc gtcccaagta ttatgagggt tattattgac aatgccgttg tcgccggctt
      841 gttcaaaagt gaattcggta tatttcacat tgggggcatt taactcatct ttgagaatgc
      901 ggtagttaat gcgagaacct tttaccggcg agactttgtc ctctttacta tggaaaaacc
      961 aaatcggtat atgtttaatt gccgccaaat tcgttttggt ggcattgccg tccacccatt
     1021 cgttggtgta ggcttcacga ccgctcatta ataatgctcc ggcaaagaaa ttcgggcgag
     1081 ccaacaacag atttaagccc ccttcagcac ctcgagataa gccattgata taaatgcgtg
     1141 tggtatcaat ttgaggattg gcttttaggg tttcatcaat catttttaac acgagattga
     1201 tacgattttc cgtttgccag tgaataccgc ctttttgccc tttggcggca ttatcaaatg
     1261 ggtcgaatac ttggtcgtat tgcggagcaa gtacaaaact tggggcataa ggcagggtgg
     1321 caatcgcccc tttactggaa agcaggtgag caaggttatc cgaaccgact tgccctgaac
     1381 cgtgtaggaa aatcacaagc gggtattttt caccattttt ttgcaaaggc gaatagagac
     1441 ggtagttcag tttatttcca gactggtcta agctcacttg ttttgcgaca aaatcatcga
     1501 gataaggggt tttgattttg tcgggttcag cggtttgtgt ccattcagtc ttgcctagag
     1561 ttgtgccgct ggttaattta agcaagcctt tttgttccgc cgaatagatt aggcgatttg
     1621 caaaaaactc tggaattttg accgcttgta ctttctctgt ttctacaatg ttgccgtttt
     1681 catctttgga tttaaatttc atcggcgctt tattagcatc ttttacgcta taagttgccg
     1741 catttttatc tgccgtatcc aattcaataa tcacaaattt accggcttgc ggaagtgcgg
     1801 tcacttttgg ctcattattg acataggctt tgagcaccga acggttttct ttctccgcac
     1861 tatctaatgc cgttttgacc tgatacagtt ggcgaagatc gctgcctgat aagacattat
     1921 caggatattc caatgccacg ccgttaattt tttgaccttg agcggtgatt tcggcaagca
     1981 acgtaatatt cgctgattta ttttcctgaa tcatatcctc tgcatagagg gagtgagaaa
     2041 gtaatagcgt aaataaaagt gtttttttca gcataggttt ctcctaaaaa aatccacctc
     2101 ataagaggtg gattttaagc agtaacagag tgatattaaa cgaataacat ccaaataatg
     2161 ccaacaacgg caaaggctat tacaccgaaa atagtcgtta aaaacgtcca agttcttaag
     2221 cctacggata caggaacatt gaagaatttc gtccataccc agaagagtga atcattcacg
     2281 tgggataatg tcatagaacc tgcaccaata gctgcagtaa gaagtgctaa ttgcagatca
     2341 ttataacctg ctgctacagc tgcaggagct agaatagttg tagtagtaat agttgccacc
     2401 gttgcagaac cttgtgctgc acgcaataat gcagaaagta tgaaagaaag aaccaataac
     2461 ggtaagcccg tgttggctaa tacatctgct aatgctttac ctacgccact ggcatctaac
     2521 actttaccga acataccgcc tgcaccggtg attaaaataa tccccgcaat cggtgctaac
     2581 gcagactcca gtacttcttc taatttatgc ttagtccaac cgtatctaaa acctaataca
     2641 taagcagaaa gacaaacagc cacaaataag gcaaacggag agcttcctac catactgaat
     2701 acttcacgca cgatatgttc tttcggcaag gttgttgccg ttactgtacc taacataatc
     2761 aggattagcg ggaaagaaat cattgctaac actttaccaa aagaaggtag ttcaatgcct
     2821 ttctctttaa tcaaatcttt ttggtgctgt aaaagatcgg ttgaagttac tacttttacg
     2881 aaattatttt ttgtaaagaa cggaataaat ttttgaccga ttaaatatgc aggtagcgct
     2941 gctactaagc caattcccaa tacaaagcca atatctgcgt ttaagttttg agcacccgct
     3001 acaatacctg gatgaggtgg aactaaaacg tgaacaagta acatcgcaag cccgattggc
     3061 aaagaataaa caaggatatt tccccctaaa cgtctcgcta cactaaaaat aatcgggagc
     3121 aatacgataa aaccaacatc aacgaaaaca ggaataccga atataaaacc tgtcatacct
     3181 aatgcaaagg gagcgttttt ctcaccaaaa cgctgtaaca accaatttgc taatacatcc
     3241 gcaccgtgag atttttcaat gattttccct aacattgctc ccaaacctac tatgagtgca
     3301 acgctaccta aagtacttcc catacctgaa atcaaggttg ggatcacttt actcattgga
     3361 atacctgcag ttaaggcaac tgcgatactc actaacccca atgctaagaa tgggtgtact
     3421 ttacctttaa tcataatgac cagaagagca actacgcttg ctaatccaat caaaattaac
     3481 atttcatttg acataaagtt tccttctaaa tgttgtattc acgtatttat ccgctaatca
     3541 tcgtggttat ttatactgct caaaccaacc tagtcctgag gtggtttccg ctcgtggttt
     3601 atactcgcaa cctacatagc cttcataccc gatttcatcg agtttgttga agatatattg
     3661 gtagttaatt tccccttcgt ccggttcgtg gcgatctggc acaggggcaa tttgaatgtg
     3721 ggcaaattta cccttgagtt tgtcggttag gcgagataag ttgccgtcca cattctgtgc
     3781 gtggaagtag tcaagctgta caaacacatt gtctcggttt accgcttcca caatttctag
     3841 cgtatcaaac tggcttttaa gcagataatt cggtttcact tcagggctta gggcttcaag
     3901 caagacgtta atgccgtgcg gtttgaattt atcggcggca tattgcacgt ttttgataaa
     3961 ggtttgttta taggcttcac gatcttcgcc ttctggcacg accgctgcca taacgtgaac
     4021 attctcacaa cctaaggcga gggcatattc caacgctaaa tcaatatcac gatggctatc
     4081 agcttcacgc cccggaatgg cagaaacacc ccattcacct tgagcaacat ctccggctgc
     4141 ggtattgaac aacacctgtt ttaagccata ttgctcaagt ttggctttca attctgctgc
     4201 cggataatca taaggccata aatactcgac atatttaaat ccagcgttgg cagccgcttc
     4261 aaaacgatct aaaaacggca cttcgttaaa catcatcgta aggtttgctg caaatttagg
     4321 cattttattt tcctctgttt ttcagatcat tgacttcgtc atcggtgaga taacggactt
     4381 tttgaccttg tagaataaaa tagagcttgg aggtttcttc caactcttct gtgttatcca
     4441 ccgcatcacg caagtctgaa cctgtgacta ccaccccgtg atttgccagt aaaaaggctt
     4501 tgccgtctaa ggctcgttcc cccagctctc gggcaatgtt gatgtcgccc ggacggtagt
     4561 aaggaatgac cggcaatttt cctacccgca tcacatagta aggggtgaaa gcacgcatag
     4621 cgttgtcggt atccaaattt tccaagcaag aaagtgcggt taaataagtg gaatgcagat
     4681 gcactattgc ttggcaagcc ggatttttct gatacatcgc caagtggaat accgattctt
     4741 ttgaaggttt atctcctgaa agaacattac cctgcatatc caatacggaa aggcggtctg
     4801 cctctaaacg cccgagcgaa gagccggttg gggtaactaa aatgcgattt tcatctaaac
     4861 gcacggagag gttgcccgca ccgccaacgg tatagccccg ctcaaacagc gagcgggcaa
     4921 gattgaccat ttcaatcttt tgttcttgtt ctgtcataca aacatctcct gagcaaaact
     4981 gaagaaatct tctttaccga agttacccga tttcaatgca agaaaaatcg gtttttcgac
     5041 cgcttgtaac cacggcacgc ccggtgcaat ttgtttgccg atgtggaaac cgctgaatcc
     5101 aagttgctga accacaatgc tcgaagtttc accgcctgcg gtaataaagt tagttacgcc
     5161 tgcggcttgt aatttttcgg ctaatttggc aaaggtattt tcaatggcgt ggctggcttt
     5221 atcgccgccg aattgggttt gaatctcttt caatttttca gggggaacgg tggcataaac
     5281 cattggggca aagtcaccgt ctaggtttgc aatcacccat tgataaagcg tttctacata
     5341 gttcggatcc tgaatggctt tttccgcttc taagaaataa cttggggctt tttgctggta
     5401 agccgccact tgggtattgg tcattaccga gcaagaaccc gaaagcacaa cggttttgcc
     5461 tttgcttggt acaaacgcat tcgtgccttt tttgccgcct gaacggtaag ccgccatata
     5521 agcccctaag cctgaaccgc ctgtgactaa ttgaaaatcg gcaaccgctt cggcaagcac
     5581 cgcaagttgt gaattatcgg cagcatctac taccgcatat ttgtagcctt gctgtttcaa
     5641 ttcagcaaaa cgggctttca cacgctctgc cccttgaatc acatcagcat aggcaaccaa
     5701 gccggttttg ccttgagatt gtgcatccat taaacgcacc aagttggcat ctttcatcgg
     5761 ggtaatcggg tggttacgca taccagattc attcagtagc acatcgccca caaacaaata
     5821 gccgttgaaa atggttctgc cattgacagg cagagccggt gtgactacgg taaaatcggt
     5881 atctaatgcc gccattaggg catcggttac cggcccgata ttgcctttct ccgtgctgtc
     5941 aaaagtggaa caatatttaa agtagaattg cgagcaacca ttttgtttca gccaatctaa
     6001 ggcttgtaat gattgttgga ttgcttcttc cacagggtta gagcgagatt tcaggctaat
     6061 cacaatcgca tcaacttgac tgttaagtgt agttttcggc acaccattca tttgcaccgt
     6121 ttttagcccg ttttcggtta agaaactggc gatgtcactc gcaccggtga aatcgtctgc
     6181 aataatgcct aacatcttat ttcccctctt ttttaggtaa ttcgatacca ctgaaaattt
     6241 taatgaccgc actatcatct tccttgccgt aacctgcgtt gctggcttcg gtaaacatag
     6301 aataagcggt gctggctaag tgaagcggga aacgcaaaga ttttgcggtg tcgttcacca
     6361 atcccaaatc tttcacaaaa atatccacca tagagagtgg ggtgtaatcg ccctcaacaa
     6421 cgtgtttcat tcggttttca aacatccaag aattgcctgc cgcattggtt acaacgtcat
     6481 acattaaatc aagcggaata ccggcacgag ccgccaatgc cattgcttct gcacctgcgg
     6541 caatatgcac accggcaagt aattgatgga taatttttac cgttgcaccc aagccgactt
     6601 cttcgccaat gttatacact ttgcctgcaa ccgcatctaa taccggtttt aatttctcga
     6661 atgcctcgct ggaacctgat gccatcacag tcatttcacc ttgtgctgct tttgctgccc
     6721 cacccgaaac cggagcatct aacatcacca agcctaaatc tgttaacttc tgtgaaatat
     6781 tttttgcatc ttgtgcagaa atggtggaag ataccatcac caccgtacct tgtttcaaat
     6841 gttgggctaa gccgttttca ccgaataaca ccgcattcac ttggttggca ttaaccacca
     6901 acaacaacac cgcatctaat tcagcggcaa aatcgaccgc acttgtgccg actttcttag
     6961 cacctgctgc ttttaaggtt tccagataat tcgggttaag atccacaccg tatgtgtcta
     7021 atccggcagc gatacaagat tttgctgctc ccatacccat tgctcctaag ccgatgacgg
     7081 ctacgctata tttttgactc attgtttatt ccctcaaata aattaatgaa cttgatggcg
     7141 gtatattaac caatagaaaa gataaaatct gtgatgaaga tcacaaatat taacaataaa
     7201 tgtgaatttt tgtgatttta attttggaaa ctaaatctga tataatctct tttaacaatt
     7261 aaaataggtt aaaaaatgat ccccttagag agacaacgag aaatactacg acttattaat
     7321 aataatggtg tcgcaagtat taattttctg gcagaacagc taaaagtatc acatatgaca
     7381 attcgccgtg atattcaaaa gctggaagag caaggtaagg tatccctagt atctggtgga
     7441 gttcaatctc ttgaacgatt aatcatggaa ttaccacacg atgataaatc aatgttattc
     7501 caagagcaga aatcagctat aggatcttta gcatcacagc taattccctc aaacacaact
     7561 atttatcttg atgctggaac tactacatta gaaatagctc atcggataat cgatagaaat
     7621 gacttactag ttgtaacaaa cgattttgtt attgctcact atcttagtaa ttcaggaaaa
     7681 tgtgatttaa ttcatatagg aggaagtgtc tgtaaagaaa acctatcgac aactggttca
     7741 cttgcagcag aatttctgaa aaacctatca atcgatatgg catttatatc tacttcatcc
     7801 tggaatttaa aaggtctaac aacgccgaac gaaaataaaa tccccgttaa aaaagcaatt
     7861 ttagaggcat ccaagcaaaa cattttagtg acagactctt caaaatatgg aaaagtcgca
     7921 actttttttg tttgccctct tactgtattt gaccagatta tttgtgataa aggattattt
     7981 gagaatgtac gagacgcact gaaagggcta agtattaaat tagatttagg ataatcttct
     8041 taccaatttt ttctgagaac gacaagcaat caaaaagtat tatttttgca aataagatat
     8101 taatagggaa agttaacaat cctagcaacg gattgttaac tgattttcgg ctggtactaa
     8161 taaaatagga atattaacgt ttaccaaaca tactgcctaa gccaccgaga ccgcctagac
     8221 caccacccat cataccttta acaccacgca tcatttttgc cattccgcct ttacgcattt
     8281 ttttcatcat acgttgcata tcatcaaact gcttgagcag cttatttaca tcttgcacct
     8341 gtgtgccgga gccaagtgcg atacggcggc ggcgtgagcc tttaataata tccggatttg
     8401 cccgctcttt taaggtcata gaattgatga tggcttccat tttcatgaac attttatcat
     8461 caacctgact tttaacgtgg tcaggcaggt ttttcgcccc cggtagttta tcgagcatcg
     8521 acatcattcc acccattttt ttcatttcga taagctgctc acggaaatct tccaaggtaa
     8581 attcatcgcc ttttttgaat ttggcggcaa ttttttccgc tttttcttga tcaaccgagc
     8641 gttgaatatc ttcaattaaa gacattacat cgcccatacc taaaatgcgg gaagccacac
     8701 ggtcagggtg gaacggctca agggcatcag ttttttcacc cacacctaag aatttaatcg
     8761 gtttaccggt gatttggcga atagaaagtg ccgcaccgcc tcgggcatcg ccatcgactt
     8821 tggtaagaat aacaccggta agcggcaacg cttcgttaaa ggcttttgcg gtgttggcgg
     8881 cgtcttgacc ggtcatcgca tcaaccgtaa atagcgtttc aatcggattc agcacagcgt
     8941 ggatttgttg gatctcttcc atcatttcgc catcaacgtg taaacgaccg gcagtatcca
     9001 caatcaatac atcaaagaaa ttaagcttag cgtgcttcaa tgccgcttct gcaatcgcta
     9061 ccggtttttg gctggtttcg gtcgggaaga agtccacttt taacgcttca gcaagggtct
     9121 gtagctgctt gatagcggca gggcggtaaa catcggcaga aactaccaac acctttttct
     9181 tgtggcgttc tttcaggaat tttgccaatt tgccgacaga ggtggtttta cccgcacctt
     9241 gcaaacctgc cattaaaatt accgcaggcg gttgggccgc aagattcagc tcctcgttgg
     9301 cttctcccat tgccgtttct aattcgcttt gtacgatttt taagaactct tgccccggcg
     9361 tgaggctttt gtttacctca acccctaacg cacgctcttt gaccttgttg atgaaatcac
     9421 gcacaacagg gagggcaaca tccgcctcca acaatgccat acgaacttca cgcagggtat
     9481 ctttaatatt atcttccgtt aggcgacctt tgcccgtaat gttacgcagc gttttagaca
     9541 gtctgtcaga gaggttttca aacatatcag ctttctcaat ttgcaaaatt ttaggaaaaa
     9601 agtaccgctt gtgagcggaa agttgccgcc attatacaaa aaaaagcgtt acggttatag
     9661 ggaaataaaa aaacggcata gcgattaaac tatgccgtaa aatttgacga tattcgagga
     9721 atcattattt agttatattg tgcgatcata atactccgaa ttgcgtataa aagtgaagtg
     9781 atattttcaa atttatgtta gaaatgagag gtaaattaca aaattaaagg ggatattatt
     9841 gtttgaaaaa ataagagcga aaagaaaacc ctgatgattg ctcatcaggg cttc
//
LOCUS       DANLXF010000082         9887 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.082, whole genome shotgun sequence.
ACCESSION   DANLXF010000082 DANLXF010000000
VERSION     DANLXF010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9887
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.082"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            9..3167
                     /locus_tag="PXD66_001306"
     CDS             9..3167
                     /locus_tag="PXD66_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDL6319606.1"
                     /translation="MITINENTIEQSAIATLQSLGWDYTYGKKILAGLEHEWRERTAE
                     VILKPLLAQAIAKFNPNLPACEVENVVAQVCRADSGDLAERNRQAYDWLRNGVKITYQ
                     LYGEQVSDVVQLIDFQRPENNDFRIVNQLDISGKKGKRIPDLIGFVNGLPLVVFELKN
                     PLKENADIGKAFAQLQTYKDEISDLFVFNQALVISDGIVARIGSLTADFDRFTPWRVV
                     DEKNQSKRIVFEDELTALLQGVMTPKNLLDYVQNFVVFERDGKNRLIKKIGAYHQFYG
                     VNEAVDCTLLAATGNRKIGVFWHTQGSGKSLSMLFYAGKVLSQSSLKNPTLVLVTDRN
                     DLDGQLYATFCGGEALLKQTPIQADGRDELRSALASRSAGGVIFTTIQKFGLMEGELA
                     HPVLNERENIIVITDEAHRSQYGFSQKINHKGEYREGYAKHLRSALPNASFIGFTGTP
                     IELDDKDTQEVFGKYVSIYDFEDAVEDGATVPIIYEPRQISLGESGEFSKVMEEAQQL
                     IDDDENSYNFRLREKLHSVDSRLQKMAEDIIAHYDERTKQQDGKAMVVVMSRAICVKL
                     YEKITALRPEWHSNDVLQGSIKIVMTSNASDPAEWQKHNQDKKTLEKRFKDPDDPLNI
                     VIVRDMWLTGFDAPCCNTMYIDKPMSGHNLMQAIARVNRVFRNKSRENGGLIVDYVGL
                     TDELEKAMKQYTNAGGKEKPVRDISAVLEKMVEHITVIRGQFATPIDGQAVDIAKMLQ
                     ISEPPKLLNAILTSANHILALDRIQPPDNTAKDKTPRKNAFLQSVRLAKKGYALCGAL
                     KAVEPYKQELAFYDAVRATIIKNSTAPRNSSSENDRLLQLTALMNRAVQSDGVVDLFD
                     LLKKDRPNINLLSDEFLETVKNSPTKDLWLSAMERYLASQLREQSGANLATKKAFEQK
                     LKEAMNQYHNHNLSVLEILEELIALAKEFEARQKRGEALGLSPAEMAFYDALARNESA
                     VREMGDEVLMKLAKDITDKLRKSVTVDWQYKDSVRAKMRTLIRIALRTYKYPPDLQAE
                     AIEFVLQQAEEIAGEFAITETA"
     gene            complement(3201..6092)
                     /gene="glnE"
                     /locus_tag="PXD66_001307"
     CDS             complement(3201..6092)
                     /gene="glnE"
                     /locus_tag="PXD66_001307"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HDL6319607.1"
                     /translation="MLTTLIPLSQLNLPPNLQNSEQIQPLVTAISLSNFVHQTLQKQP
                     DLLAEWLAKFPTPDDCCNYTERLAKILARVENEEMLGRVLRQFRHRELARLSFIQSNK
                     LATVELVFQHLSDLAESLILAARDWLFQRCCAEYGTPKNTLGETQELLILGMGKLGGR
                     ELNFSSDIDLIFTYPDIGETEGGRKSIENSKFFTRMGQRLIKVLDEITADGFVYRTDM
                     RLRPFGDSGALVLSFTAMEDYYQEQGRDWERYAMIKAKILGEDSQNLNHRYLKQMLRP
                     FVYRRYLDFSAIQSLREMKEKISREVVRRNLTDNIKFGAGGIREVEFIAQTFQMIRGG
                     RDKILQERSLLKVLPRLAELNLLTQTQVETLHNAYIFYRQIENVLQAIDDKQTQTLPT
                     DEASQARLVFACQSYYQQDLYSEKTHWVEHSFNDWQQFMAVLSQYQQAVRQIFNEIIG
                     EEETQANCNPVNEKLAEWKDILHYNIRLEDLSAVLKGYPKVAENDYTEIFRHFSTTFQ
                     DWVKRPIGVRGREVLRNLMPRIADSIFKGEDHLLLLPRVLNIVDKITTRTTYLELMLE
                     KEQILPQLLALCSKSVMIAEQIARYPMLLDELMSHRGLTDVQAFEKYQSALQDYLIRI
                     PEEDEEALIDGLRQFKQTQILRIAAADILGVLLVMKISDHLTYLAEAIINAVVNMAWK
                     QVSQRFGVPEHLEADEKGFAVIGYGKLGGIELGYNSDLDLVFLHNAPEDSQTVGGKKE
                     ISSHQFYLKLAQKINSIFNLNTSAGVLYEVDMRLRPSGEAGLLVSTFNAYEHYQKNEA
                     WTWESQALVRTRCVFGAENLKQAFEKIRQSTLAQPRASGQLRQEICEMRQKMYQHLSS
                     HSAEQFHIKQDQGGITDIEFIAQYLVLAHSHQHPKMAVWSDNVRIFDSAVECGILSSE
                     QSEQLQHCYTALRNKIHHLNLLRKDSVVDASEFTTERAFVREMWQRLLA"
     gene            complement(6264..6545)
                     /locus_tag="PXD66_001308"
     CDS             complement(6264..6545)
                     /locus_tag="PXD66_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HDL6319608.1"
                     /translation="MYYVIFAQDNPNMLEKRLEVRPAHLARLEKLQAEGRLLIAGPNP
                     TEDGSSVTGSTVIAEFNSLEEAQQWASQDPYVEAGVYSDVIIKPFKRVF"
     gene            complement(6547..7011)
                     /gene="yciA"
                     /locus_tag="PXD66_001309"
     CDS             complement(6547..7011)
                     /gene="yciA"
                     /locus_tag="PXD66_001309"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HDL6319609.1"
                     /translation="MKDKPYGKAPEGKLILRTLAMPSDTNANGDIFGGWIMSQMDLGG
                     AILAKELAKGRVVTVTVDKMIFHRPISVGDVVCCYGKLMHIGRSSMQVKVEVFIKQVY
                     EGNRERYLVTEALFTYVAIDKEGKPRAVPRENNPELDEALSLIDLTPEKQGE"
     gene            complement(7001..7558)
                     /locus_tag="PXD66_001310"
     CDS             complement(7001..7558)
                     /locus_tag="PXD66_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HDL6319610.1"
                     /translation="MKQLLEFIPLILFFVVYKMSGIQMASIALVIATIVQMIALKMLY
                     GKIEKQQIIMGGSVVFFGLLSAYFNEVKYLQWKVTIVYALFALVLLVSQFVFKTPLIK
                     KLLGKEIQLPEAVWNKINFGWAVFFLICMLLNIYISQYLSEEIWVDFKSFGIIGLTLV
                     ATVITGFVLYPYLKDAEKQEQKNER"
     gene            complement(7567..8376)
                     /locus_tag="PXD66_001311"
     CDS             complement(7567..8376)
                     /locus_tag="PXD66_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL6319611.1"
                     /translation="MAQALLYFTTKYLQSIEGYFMSIKFVDLLQQSWNFMRNQQAFSL
                     FAIVTIVVVQLVFILVSPNSPELMAESQPQQLQIDASKMVSILLPTILLGVVNLLINV
                     LMIFNIQSINDGNYRQFFQNAGNALKHFLPVLLLQFVMVLPLSLGASFAMASPETVII
                     ALPVLVVGFYFFIKLSLVIYAYLLEKPQKSLSESIKFTLQLSRGKMLPLILFCVISYL
                     LPGMLSRLFTVFGNDFIGIFITIVLSAVINVFMAIFSFRFYQVYRQMPAVR"
     gene            8447..8884
                     /locus_tag="PXD66_001312"
     CDS             8447..8884
                     /locus_tag="PXD66_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HDL6319612.1"
                     /translation="MLSLFDAGQRYLNTLPNQKKLANFMPDYHIIRLVKFSSKVMPAF
                     ACFALVWQYCFTDPTQSILANSVLTAVFAISIPFQGLYWLGKRARSPLSLSLLEWYES
                     LRQKLISAQVKLEETQAVPSFQDFANLLKLAEETWGDEYFDEL"
     gene            complement(9169..9591)
                     /locus_tag="PXD66_001313"
                     /pseudo
     CDS             complement(9169..9591)
                     /locus_tag="PXD66_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(9721..9887)
                     /locus_tag="PXD66_001314"
                     /pseudo
     CDS             complement(9721..9887)
                     /locus_tag="PXD66_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019518307.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT         2903 a         2119 c         2067 g         2798 t
ORIGIN      
        1 gagttgtgat gattaccatc aacgaaaaca ccattgaaca atccgccatt gcaactttgc
       61 aaagcttggg ctgggactac acctacggca aaaaaatttt ggcaggttta gaacacgaat
      121 ggcgggagcg aaccgctgag gtgattttaa agccgctttt ggcgcaagcg attgcaaaat
      181 tcaacccgaa tttgcccgct tgtgaggtgg aaaatgtggt ggcacaggtg tgccgtgccg
      241 acagtggcga tttggcggag cgtaatcgtc aggcttatga ttggctaaga aatggggtca
      301 aaatcactta tcagttgtac ggtgagcagg tgtctgatgt ggtgcagctg attgattttc
      361 agcgtccaga aaataacgat tttcgcattg tcaatcagct ggatattagc ggcaaaaaag
      421 gcaaacgcat tccggatttg attggctttg ttaatggctt gccgctggtg gtgtttgagc
      481 tgaaaaatcc gctcaaagaa aatgccgaca ttggcaaggc gtttgcccaa ctgcaaacct
      541 ataaagatga aatttctgat ttgtttgtgt ttaatcaggc actcgtgatt tcagacggca
      601 ttgtcgcccg catcggttcg ctgaccgctg atttcgaccg ttttacccct tggcgtgtgg
      661 ttgatgaaaa aaatcagagc aaacgcattg tgtttgaaga tgaactcacc gccctgctgc
      721 aaggcgtgat gacacccaaa aatctcttgg attatgtgca gaattttgtg gtgtttgaac
      781 gggacggcaa aaaccgctta attaagaaaa tcggagcata tcatcagttt tatggcgtga
      841 atgaagcggt ggattgcacc ttgcttgccg ccacaggcaa ccgcaaaatc ggcgtgtttt
      901 ggcatacgca aggttcgggc aaatcgcttt cgatgctgtt ttatgcaggt aaagtattaa
      961 gccaaagcag cctgaaaaat ccaactttgg tgttggtaac cgaccgcaac gatttggacg
     1021 gtcagcttta cgccactttt tgcggtggcg aggcactgct caaacaaacg ccaatccaag
     1081 ccgatgggcg agacgaactc cgctctgccc ttgccagtcg ttcggcaggc ggtgtgattt
     1141 ttaccaccat tcaaaaattc ggcttaatgg agggtgagct tgcccaccct gtattaaacg
     1201 agcgggaaaa catcattgtg attaccgatg aagcacaccg ctcacaatat ggttttagcc
     1261 aaaaaatcaa ccacaaaggc gagtatcgtg agggttatgc caagcattta cgcagtgcat
     1321 tgccgaatgc ctcttttatt ggctttactg gcacaccgat tgaacttgat gacaaagaca
     1381 cccaagaagt cttcggtaaa tatgtctcga tttatgattt tgaagatgcg gtggaagatg
     1441 gggcaaccgt gccgattatt tatgagccac gccaaatcag cttaggcgaa agtggcgagt
     1501 tttccaaagt gatggaagag gcacaacaac tgattgatga cgatgaaaac agctataact
     1561 tccgcctgcg tgaaaaactg cacagcgtgg atagccgttt gcaaaaaatg gcggaagata
     1621 ttattgccca ttacgatgag cgtaccaaac agcaagacgg caaggcgatg gttgtggtga
     1681 tgagccgtgc catttgcgtg aaattgtacg aaaaaatcac cgcacttcgt ccagaatggc
     1741 actcaaacga tgtgcttcaa ggcagcatta aaattgtgat gacaagtaat gccagcgacc
     1801 ctgccgagtg gcaaaaacac aatcaggata agaaaacctt agaaaaacgc tttaaagacc
     1861 cagacgatcc gctcaacatc gtgattgtgc gggatatgtg gctgacaggc tttgatgcgc
     1921 cctgctgtaa cacaatgtac attgataaac caatgagcgg acacaacctg atgcaggcaa
     1981 tcgctcgggt aaatcgggta tttcgcaaca aaagccgtga aaatggcggc ttgattgtgg
     2041 attatgtggg cttgaccgat gagctggaaa aagcgatgaa gcagtacacc aacgcaggcg
     2101 gtaaagaaaa gccggtgcgg gacatttcag cggtgctgga aaaaatggtg gaacatatca
     2161 cggtcattcg tgggcaattt gccacaccga ttgacggaca agcggttgat attgccaaaa
     2221 tgttgcaaat cagcgaaccg cctaaactgc tcaatgcgat tttgacatca gccaaccata
     2281 ttcttgccct agaccgcatt cagccacctg ataacactgc caaagacaaa accccacgca
     2341 aaaacgcctt tttgcaatcg gtacgcttgg cgaaaaaagg ttatgctttg tgtggggcat
     2401 taaaagcagt tgaaccctat aaacaggaac tggcgtttta tgatgcagtg cgtgccacca
     2461 ttatcaaaaa cagcaccgct cctcgcaatt cttcgagcga aaatgaccgc ttgttgcagc
     2521 ttaccgcctt gatgaatcgt gcggtacagt cggatggtgt ggtggattta tttgatttgc
     2581 tgaaaaaaga ccgcccaaac atcaacctgc tttctgatga gtttttggaa acagtaaaaa
     2641 acagcccaac caaagatttg tggctatcag caatggagcg ttatcttgcc tcacaactgc
     2701 gagagcaaag cggtgccaat cttgccacaa aaaaagcatt tgaacagaaa ctcaaagagg
     2761 cgatgaacca ataccacaac cacaatttaa gcgtattgga aattttagaa gagctgatcg
     2821 ccctcgccaa agagtttgaa gcccgccaaa aacgagggga agcgttggga ctaagccctg
     2881 cggaaatggc gttttatgat gctttggcac gtaatgaaag tgcggtgcgg gaaatgggcg
     2941 atgaggtatt gatgaaactt gccaaagaca ttaccgataa attacgcaaa tcggtcaccg
     3001 tagattggca atataaagac tcggttcgtg ccaaaatgcg aaccttaatc cgcattgctc
     3061 tgcgtaccta taaatacccg cccgatttac aggcggaagc gattgagttt gtgttgcaac
     3121 aagcggaaga gatcgccgga gagttcgcta tcaccgaaac ggcgtaatct atattagggc
     3181 gtgcaatgta cgccctaaat ttatgctaat aacctctgcc acatttcccg cacaaatgct
     3241 cgctcggtgg taaattcact tgcgtccacc acgctgtctt tgcgtaataa gtttaggtgg
     3301 tggattttgt tacgcagtgc ggtgtagcaa tgttgtagct gttcgctttg ttcggaactt
     3361 aaaataccgc attctactgc gctgtcgaaa attcggacat tatccgacca tactgccatt
     3421 ttcgggtgtt ggtggctgtg ggctaatacc aaatattggg cgataaattc aatatcggta
     3481 atacctcctt gatcttgttt aatatgaaat tgctcggctg aatgcgagct taaatgttgg
     3541 tacattttct gtcgcatttc gcaaatttct tggcgtaatt gaccgcttgc acgaggctga
     3601 gcaagcgtgc tttggcggat tttttcaaag gcttgtttga ggttttccgc cccaaataca
     3661 catcttgtcc gcacgagggc ttggctttcc cacgtccacg cttcattttt ttgataatgc
     3721 tcgtaggcat tgaaagtgct gactaaaagc ccagcctcgc ctgatgggcg aagtcgcata
     3781 tccacttcat atagtacgcc ggcactggta ttaaggttaa aaatcgagtt aattttttga
     3841 gccaatttta agtaaaattg atggcttgaa atctcttttt taccgccaac agtttggcta
     3901 tcctctgggg cattatgcaa gaaaaccaag tcgagatctg aattatagcc cagttcaatc
     3961 ccgcctagct tgccgtagcc aataactgca aacccttttt catcagcctc taaatgttcc
     4021 ggcacaccaa agcgttggct gacttgcttc cacgccatat ttaccaccgc attaataatc
     4081 gcttctgcta agtaagtaag atgatcgctg atcttcatta ccagcagcac gcctaaaata
     4141 tcagcagcgg cgatgcgtaa aatttgggtt tgtttgaact gacgtaagcc atcaatcagt
     4201 gcttcttcat cttcttcagg aatgcggatt aaataatcct gcaaggcaga ttgatatttt
     4261 tcaaaagcct gcacgtcggt taagcctcta tggctcatca gctcatcaag tagcattgga
     4321 tatcgagcta tctgttccgc aatcataacc gacttactac acaatgctaa aagttgaggc
     4381 agaatctgct ctttttcgag cattaattcc aaataggtag ttcgggtggt gattttatcg
     4441 acaatattca acacgcgagg caataacaat aaatgatctt cacctttaaa aatgctgtcg
     4501 gcaattctag gcattaaatt gcgtagcacc tctcgccctc ttaccccaat cgggcgtttt
     4561 acccaatctt gaaaggtagt agaaaagtga cggaaaatct ccgtataatc attttctgca
     4621 acctttggat aaccttttag cactgcggat aaatcttcaa ggcgaatgtt atagtgcaaa
     4681 atgtctttcc attcggctaa tttttcatta accggattgc agttggcttg tgtttcctct
     4741 tcaccgataa tttcattaaa aatctggcgt actgcttgtt gatattggct caatactgcc
     4801 ataaattgtt gccaatcgtt aaaggaatgc tccacccaat gcgttttttc gctatataga
     4861 tcttgctgat aatagctttg gcaagcaaat acaaggcggg cttgcgaggc ttcatcagtg
     4921 ggtagggttt gagtttgttt atcgtcaatc gcttgtaata cattttcaat ttgacggtaa
     4981 aaaatatagg cattgtggag cgtttccacc tgcgtttggg ttaataaatt taattctgcc
     5041 aagcgtggta gcacttttaa caggctacgt tcttgtaaaa ttttatctcg cccgccacgg
     5101 atcatttgga aagtttgggc gataaactcc acttcccgaa tcccaccggc accaaattta
     5161 atattatcgg ttaaattacg gcgaaccact tcacggctga ttttttcttt catttcacgc
     5221 aacgattgga ttgcactgaa atccagataa cggcggtaaa caaaagggcg gagcatttgc
     5281 tttaaatagc gatgatttaa attttggcta tcctcgccta aaatttttgc tttaatcatc
     5341 gcataacgtt cccaatcacg cccttgctct tggtaataat cttccattgc agtaaaactc
     5401 agcaccaagg ctccgctatc accaaagggg cgaagtcgca tatcggtacg atacacgaaa
     5461 ccatcagcgg tgatctcatc taatacttta attaaccgct gccccattcg ggtaaaaaat
     5521 ttgctgtttt caatcgattt gcgtccacct tcggtttcac caatatcggg gtaggtaaaa
     5581 atcaggtcaa tatcggaaga aaaattaagc tctcgtccgc ccaatttgcc catacctaaa
     5641 atcagcagtt cttgtgtttc gccaagagtg ttctttggcg tgccatattc ggcacagcaa
     5701 cgctgaaaca accaatctcg agcggctaga atcaggctct cggctaaatc cgacaaatgc
     5761 tgaaatacca attctaccgt cgctaattta tttgactgaa taaagctcag acgagccaat
     5821 tcacggtggc gaaattgccg caaaaccctc cccaacattt cttcattttc cactcttgct
     5881 aatatttttg ctaaacgctc agtataattg cagcaatcat caggcgtagg aaacttggca
     5941 agccattctg ccagtaaatc cggctgtttt tgcaaggttt gatgcacgaa attggaaagc
     6001 gaaatcgccg tcacaagcgg ttgaatttgc tcagaatttt gcaaattggg cggtaaattg
     6061 agttgggata agggaattag ggtggtaagc ataaatttag gctcaaaatt gaaaatagat
     6121 agaattttac gctcgatcaa ttcccctctt tagcaaagag ggcttagggg agatttggca
     6181 gaggtaattt tactctcata cgaaacatta atatcgtaat caaatctccc ccagcccctc
     6241 ttttctaaag aggggagaaa aaattaaaac actcttttaa acggtttaat aatcacatcg
     6301 ctatacacac cggcttccac ataaggatct tggcttgccc attgttgagc ttcttcaagt
     6361 gagttgaact ctgcaattac agttgaaccg gttacgcttg agccgtcttc agtcgggtta
     6421 ggccctgcga ttaataatcg accttctgcc tgtaattttt ccaaacgagc caaatgagcc
     6481 gggcgaactt ctaaacgttt ttccaacata ttgggattat cttgggcaaa gataacataa
     6541 tacatactat tctccttgtt tttcaggggt taaatcaatt agcgataatg cttcgtctaa
     6601 ttcggggtta ttttctctcg gcacagcacg cggtttgcct tctttatcaa tcgctacata
     6661 ggtaaataag gcttcggtta cgagataacg ttcgcgattg ccctcataaa cttgtttgat
     6721 aaagacctcg actttcactt gcatcgaaga acgccctatg tgcattaact tgccgtagca
     6781 gcagaccaca tcaccgactg aaatcgggcg gtggaaaatc attttatcta ccgttaccgt
     6841 aacgactcta ccttttgcca actctttggc taaaattgca ccgcctaaat ccatctgcga
     6901 cataatccaa ccgccgaaaa tatcgccatt ggcgttagta tctgacggca ttgccaaagt
     6961 tcttaaaatc agtttccctt ccggggcttt tccatagggt ttatctttca tttttttgtt
     7021 cctgcttttc agcatctttt aagtaagggt aaaggacgaa accggtaata accgttgcga
     7081 ctaaggttaa gccaataatg ccaaaggatt tgaaatcaac ccaaatctct tccgataaat
     7141 attgactgat ataaatattc agcagcatac aaatcagaaa gaatactgcc caaccgaagt
     7201 ttattttatt ccacactgct tcaggcaact gaatttcttt gcctaataat tttttgatta
     7261 atggtgtttt gaacacaaat tggctcacta atagcaccaa tgcaaacaga gcgtaaacaa
     7321 tggttacttt ccattgtaaa tatttaactt cattaaaata ggcactcagt aagccgaaaa
     7381 agaccaccga gccacccata ataatttgct gtttttcaat tttgccgtag agcattttta
     7441 aggcgatcat ttgcacgatt gtagcgataa ccaatgcgat agacgccatc tgaataccgc
     7501 tcattttata cacgacaaaa aacagaatca gcggaataaa ttcaagtaac tgtttcattg
     7561 atactcctaa cgcactgccg gcatttggcg gtaaacttgg taaaagcgga agctgaaaat
     7621 cgccataaag acgttaatta ccgcacttaa aacaatggta atgaatatgc caataaagtc
     7681 attaccaaac actgtgaaca aacgagataa catccccggt aataaataag aaatcacaca
     7741 aaataaaatc agcggtagca tttttccacg tgataattga agcgtaaatt taatactttc
     7801 cgatagactt ttctgcggtt tctccaataa ataagcataa atcactaacg ataatttgat
     7861 aaaaaaataa aaaccgacta ccaacaccgg caatgcaata atcactgttt caggcgatgc
     7921 cattgcaaaa gatgcaccga gagaaagcgg taacaccatc acaaattgta acaacagcac
     7981 aggtaggaag tgctttaatg cgtttcctgc attttggaaa aattgacgat aattgccatc
     8041 gttaatcgat tggatattga aaatcattaa gacattgatc aaaagattga caacacctaa
     8101 taaaatagtt ggcaatagta tagacaccat tttagaggca tcaatttgaa gttgttgcgg
     8161 ttgcgactcc gccattagct caggcgaatt tggcgacact aaaataaaaa ccaactgcac
     8221 aaccacaatg gtgacaatgg caaacagtga aaatgcttgt tgattacgca taaagttcca
     8281 actttgctgc aataaatcga caaatttaat tgacataaaa tacccttcaa tagattgtaa
     8341 atattttgtt gtgaagtata gcaaggcttg agccaaactg cattaaccta ttgtagattt
     8401 taatcagtta gctaaaataa aagaaaaatt tgaaagagat aatcctatgt taagcttatt
     8461 tgatgcagga cagcgttacc tcaacactct gccgaatcag aaaaaattag ccaattttat
     8521 gccagattac cacattattc gcttggtaaa attttcctct aaagtaatgc ctgcttttgc
     8581 ttgctttgca ttagtttggc aatattgctt taccgatccg acccaatcta ttttagccaa
     8641 ttcggtactt actgctgttt tcgccatttc tattccgttt caagggttat attggcttgg
     8701 aaaacgggca agatcaccgt tatctttatc attattggaa tggtatgagt cgcttagaca
     8761 aaaattaatt tcggcacaag tcaaattaga agaaacacaa gcggtacctt ctttccaaga
     8821 ttttgcaaat ttactcaagc tagcagaaga gacttgggga gatgaatatt ttgatgagtt
     8881 ataaaataaa aaaagcccca cttaaaataa gtggggtaaa acttttgttc taattctgga
     8941 ggtttgaatt actgttttat tgtggggtac tgatcttttt tgcatagaga gaaagaaaaa
     9001 aatttgggcg gaaaaataac ggcgagcccc gtgaatacaa gggttcgccg ttattgtttg
     9061 gttggagcta tgcaaaaaac aaaatgagca ggattagaaa aaaacgagca gtttttgcgt
     9121 tattttttgc ataggtttga tcggttttaa aactatttta aatctgtttt aaactaaaga
     9181 atgcccgatg tgtaccactt gcccgatgat ttgtaggttt tgggcttggt ctgctgtaat
     9241 ctttattaac tcgtactctt ctcggttgtc tgaaataagc tctacaccgt cccatagcag
     9301 ttttacacgc ttaacccaaa cactgtcgcc gttttgtaca atgtaaatat tattgtcgcc
     9361 tcgtatctca ttacgactta aatcaactag tagctggtcg ccattatcaa tagtcggata
     9421 catcgagtct ccacttgccc aaaaaacagc acacttcttg gcattaacac caagcgtatg
     9481 caacaagcta tcagcataag gctcttgtcc atcaggtttt gttacacctt cattaaagct
     9541 cccaaagcct gcagatactt taatagatgc aaaactatca atcattgaga ttatgttatt
     9601 caaaacggtg cctgataatt cagatccttt gttttgctct tcaggtgtaa tctctcttac
     9661 tatgctatcc ttgattgcat ttattccact tttaaatccg agtgcttctt gggctttacg
     9721 aggcaatgaa gttatgtgat attcattccc cccacctttt actccaactg catcacgttt
     9781 tatccagagt tcttttaatg cctttcttgt aatgtttgtt gcttgctttg gaaggtcagg
     9841 caaatccaat tcttctagtt ctttggctga aaaccaaact ttatcaa
//
LOCUS       DANLXF010000083         9819 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.083, whole genome shotgun sequence.
ACCESSION   DANLXF010000083 DANLXF010000000
VERSION     DANLXF010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9819
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.083"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            125..1831
                     /locus_tag="PXD66_001315"
     CDS             125..1831
                     /locus_tag="PXD66_001315"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HDL6319613.1"
                     /translation="MNTSSFNRTWAKVILSALMRYGVKHFCIAPGSRSTPLTLAAVQL
                     QQTQYAECHTHFDERGLGFYALGIAKATDSPVAIIVTSGTAVANLYPAVIEASLSHQK
                     LIILSADRPTELIGCGVNQAIHQQGIFANYPVASVNLPKANEAYHASWLVSTIEQACT
                     TQAQQGGVLHINVPFEEPLYEADINEILNTPWLRPIQGWLNNAQTKWIDQQAIQSEVS
                     MHENWDYWRTKRGVIVVGKLPPEQGTGLKTWAETMGWCLLSDVQSCIEASLPYADIWL
                     SNETVKQRLLQADIVIQFGTQIVSKRVNEFLKAFQGEYWVVEQSHDYHNPNAHHQTRF
                     IAKAHHFLRVHPPLRQKPWLLEPLALSQFCSTFIEQQVGGSLSEASLAHNIQDVLAIN
                     GNLFIGNSLFVRLVDALCKLPEGYPVYTNRGASGIDGLIATMAGIAKGSDRPTVGVVG
                     DISALHDLNSIALLTKINQPTILFVVNNSGGALFDMLPVDSKAKDQYYRLAHNFEFAQ
                     VATMFGLEYIRPFTWADLKTKLKHAYARKGVTIVEIKVNEHDGSNMYKSLLKQISQAE
                     IA"
     gene            1880..2719
                     /gene="menH"
                     /locus_tag="PXD66_001316"
     CDS             1880..2719
                     /gene="menH"
                     /locus_tag="PXD66_001316"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HDL6319614.1"
                     /translation="MLSYQWHAQQGTPVVFLHGLLGSQQDWQAVLQHLQNFPQIRPLT
                     IDLPFHGNSKHITCADFANLRQQLHATLESLIGSRPFYLVGYSLGGRAALDYVLNMEN
                     PNLQGVVLEGANIGLSTENDRQIRRQNDRHWANRFRQEPIEQVLEDWYKQPVFADLSD
                     TERECYIDSRKHNQGEQIAQMLEATSLAKQPDYTEKLKQTDKNIVFFIGEKDQKFRKM
                     ASSHQLPTQLIANAGHNAHRANAKNFVEKLGLLILHSDSDLRSQSVKHNGAALLHKSP
                     SGA"
     gene            complement(2768..3445)
                     /locus_tag="PXD66_001317"
                     /pseudo
     CDS             complement(2768..3445)
                     /locus_tag="PXD66_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251475.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            3531..4193
                     /locus_tag="PXD66_001318"
     CDS             3531..4193
                     /locus_tag="PXD66_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HDL6319615.1"
                     /translation="MRKTHSFQQVRKIKPTWMSVSGHIPFRNGVSIPYESTLERDFLM
                     YFTYLPSVDEVVSQPTTLPFVKNGITYTYTPDFFLSFTDGRKPMLIEVKPKAKWQKHW
                     KEWKEKWKAAIRFCQENGYVFHVYDEDRIRHLALFNLNYVQRYKRIQHEQEDIDAILA
                     QVKLMGNTTIDYLLSRFFAGSLYRMKGLQIIYHLLATKQLHCNWFLPLNEFTEVWGNN
                     DE"
     gene            4183..6111
                     /locus_tag="PXD66_001319"
     CDS             4183..6111
                     /locus_tag="PXD66_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HDL6319616.1"
                     /translation="MMSNSGFTEKRYHHRLDRGRIILQKGNIYLNREDGEQYELVDYM
                     DEPSQLLVRNLNTRTTKVVSIHQLENFKMNERTDLSVDVTAISNEYWEKAQQKYEAIK
                     PLLGMDQHRPYAVKARAEDVGVNPRTLYRWLQAYNSIGSIAGLVDQKRGWQQGNSRLT
                     PEQDKLIVQVINEFYLHKQRPTTEQTIREIRRRCKIEKVESPSKETIRMRILHISEEE
                     RLRKRGQREKARNKFKPKPNSFPDADYPLSVVQIDHTPVDLIIVDSKYRKPIGRPFLT
                     VAIDIYSRMIVGYYLSLDAPSVTSVAMCIARGILPKERLLLDLGLQGSEWNAFGYPVK
                     VHVDNGPDFQALDLSKSCSAHGIHLEFRPMGRPEYGGHIERVIGTFMKEVHSLAGTTF
                     SNIKERDSYDSEKEAIMTLDEFEKWLVHYIVNVYHKRVHSALGISPEQKWKIGIFGDE
                     NEVGCGYPQLPVDEQTLLLDFLPSITRTIQHNGVTIDGLRYYDVALNMYISDSDESGK
                     SKEFLFRRDPRNISKIWFYDPKLKRYFPIPFANQAMPEMSIWEYREVRSRIANKGDKY
                     INEQQVLDGLTEMREMVAESAQRTKKARRQAERQKMHKASKPIIETKVETKAVVPVVV
                     TSNLLALDDESLSFGEVD"
     gene            6111..6998
                     /locus_tag="PXD66_001320"
     CDS             6111..6998
                     /locus_tag="PXD66_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniB family NTP-binding protein"
                     /protein_id="HDL6319617.1"
                     /translation="MQYEHVHEKFRHLVTADNQERIAFLDEPRWLGYGVAKDIMDNLV
                     SLMNKPKRPRMLNLLIVGDSNNGKTTLIRRFFDLYGQAYIDSDSNAIYPILLAEAPPS
                     ANEKELYISLLERFYVPYKPTDTIAKLRYQTIHLFREFRVKMLVIDEFHSLLVGTPRL
                     QRQVMNAIKMLCNELQIPIVGVGTRDAIRVLHTDRQHASRFDVAELPIWKLDKEFQKL
                     LFQFQGILPLRESSNLQSPELATKIHTISGGNLGNVHRLLTACAVQAITSGTEKITLD
                     IIEKNSWVQPTNGYRKIIG"
     gene            6956..8062
                     /locus_tag="PXD66_001321"
     CDS             6956..8062
                     /locus_tag="PXD66_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HDL6319618.1"
                     /translation="MGTTNKRLSQNNRIKKGVIHTPIYSNESISSWLIRASFDCGTDP
                     LTFTGFYWEKHRLWTLDLDRGLENIEPTIYDDVQALSGKIQLSEYSLFRRLYPINADE
                     TLAKGQVRWVIPRSARNRTHHIGQQYCPLCLDESPHLRNDWRLAWNFGCLKHNMMLAN
                     RCFCCGTLYQPHLLSAEKRHLNHCHQCGGKLEQGTMEMSLAEKLTLQGINQVLEKDSG
                     ICFGYKVSGKVWFDMLRYCINLLRRASDRPNHALAKFVVNLQIDVENICSPKTGLSFE
                     QLPLGERTNLLVNAYHILGMEADSIRSAIYLCGGTQKTFMFEKYPDFWQEFMALAPAG
                     PNRTRKRTKPQNESITAINRRWERLKRKLHLNNE"
     gene            8059..9483
                     /locus_tag="PXD66_001322"
     CDS             8059..9483
                     /locus_tag="PXD66_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319619.1"
                     /translation="MKINVIKPQEEPCSACGKISRIWRRYNGEGYCANCYAKWFPKKE
                     CSCCHQPKRIYLSSTVCWDCEKQTDCVRCGKAAGTFKIGKITRYGAVCNVCSRYFREE
                     RECFECGQLSRTMLRASESGHSELLCLKCFQKYRFATCKNCRRYRKVHDRDNQLCRKC
                     SEQRTSQCPKCKQDMPAGYGNICADCSRRTLLFNLIRMNVHLFRHKKVKRAYKKFIFW
                     YLAKCGVSVVLKKSDDHVLFFMRCDENWGNIPDYRELVTFFKPNGLRTHLTVLRWLLD
                     TNQVKVDESLKEEMAGLERIQSLFNKLSNSVPCIANYYTQLQHKYEAGKTSLKSVRLA
                     LQPAIDFISSESVSLLPTQVQLNSYLVAKTGQIAAITGFINHLNREYHCELEIDRSLI
                     QKMKEKQLKQQAKQRLAILYKQEKLSIKEQNELLSVVLLNLHNIWIKSPKKLSISEID
                     GIAYYVDNEKKYFIPKDIHIRLME"
     gene            9598..9804
                     /locus_tag="PXD66_001323"
     CDS             9598..9804
                     /locus_tag="PXD66_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA delta(2)-isopentenylpyrophosphate
                     transferase"
                     /protein_id="HDL6319620.1"
                     /translation="MTLKAQFREWLKKEQEHDEMIGFAHIPCILSKVDSKLAIVSGNA
                     YEKKYLRYWIKCFRFDRNISKYIK"
BASE COUNT         3033 a         1984 c         2117 g         2685 t
ORIGIN      
        1 atagaaattt tcacgaagtt ttacaaaaac attccaattt tctcctaaaa aacttcccac
       61 aataatttca ctttttgcta caatcctaac catttatcga atttatcaat cagtagagaa
      121 gaatatgaat accagttctt ttaatcgcac ttgggcaaaa gtgattttaa gtgcattaat
      181 gcgttatggc gtaaaacatt tttgtattgc tccgggttct cgctcaacac cgctcacatt
      241 agcggcagtg caacttcaac aaacgcaata tgcagagtgc cacactcatt ttgatgagcg
      301 tggtttaggt ttttatgcgt tagggattgc taaagcaacc gacagcccgg ttgctattat
      361 tgtcacttcc ggcacagcag tagcgaacct ttatcctgcg gtgatagagg caagcctgag
      421 ccaccaaaaa ctgattattc tttctgccga ccgcccaacc gaattaatcg gctgtggggt
      481 aaaccaagcc attcatcagc aaggtatttt tgccaattac ccggttgcca gtgtcaatct
      541 accgaaagcg aacgaggctt accacgcaag ctggctggtt tcgaccattg agcaagcctg
      601 cactacccaa gcacaacaag gcggtgtgtt gcatatcaat gtgccttttg aagagccgct
      661 ttacgaagca gacattaatg aaattctaaa tacaccttgg ttacgcccaa tccaaggctg
      721 gttgaataac gctcaaacca aatggataga ccaacaagcg attcaaagcg aagtctcaat
      781 gcacgaaaat tgggattact ggcgaactaa acgaggggtg attgtggtcg gcaaactgcc
      841 gccggaacaa ggcacaggtt taaaaacttg ggcggaaacc atgggttggt gcttgctaag
      901 tgatgtgcaa tcttgcattg aagccagcct gccgtatgcc gatatttggc tctccaacga
      961 gactgtcaaa cagcgtttat tgcaagcgga tatcgtgatt caattcggca cgcaaattgt
     1021 cagcaaacgg gtgaatgaat ttttaaaagc attccaaggg gaatattggg tggtggaaca
     1081 atcgcacgat taccataatc caaacgctca tcatcaaacc cgttttattg ccaaagcaca
     1141 tcactttttg cgggtacacc caccacttcg ccaaaagcca tggttgcttg agccattggc
     1201 attatcgcaa ttctgctcca ctttcataga gcaacaagtg ggtggcagct taagtgaagc
     1261 ctcacttgca cacaacattc aagatgtttt agcaattaat ggcaacttat ttatcggcaa
     1321 tagcttattt gttcgattgg ttgatgctct gtgcaaactg ccggaaggct accctgttta
     1381 caccaaccgt ggtgcaagcg gtattgatgg cttaatcgca acaatggcag gcattgcaaa
     1441 aggtagcgac cgcccgactg tcggtgtggt aggtgatatt tccgcattac acgatcttaa
     1501 ctcaattgca ttgctcacca aaattaacca gccgaccatt ttatttgtgg tgaataacag
     1561 cggtggtgcg ttgtttgata tgttgcctgt tgatagcaaa gcgaaagatc aatattaccg
     1621 ccttgctcac aactttgaat ttgctcaagt cgccacaatg ttcgggcttg aatatatccg
     1681 cccgtttact tgggcagatc taaaaaccaa actcaaacac gcctatgctc gtaaaggtgt
     1741 taccattgtg gaaatcaaag taaatgaaca cgacggcagc aatatgtata aatcattgct
     1801 aaaacaaatt tcacaggcgg aaattgccta atcacaactg cccctcgcca atcctgcgag
     1861 gggtaaaaat gtatcgacta tgctttccta tcaatggcac gctcaacaag gcacacccgt
     1921 ggttttcctt cacggcttgc tcggctcaca acaagactgg caagctgttc tccaacattt
     1981 gcaaaatttt ccgcaaattc gaccgcttac cattgacctt ccttttcacg gaaatagtaa
     2041 gcacattacc tgtgccgatt ttgccaacct gcgacaacaa ctacacgcca cccttgaaag
     2101 cctaatcggc tcacgacctt tctatttagt cggctactca ctcggtggac gagcggcact
     2161 cgattatgtg ttaaatatgg agaatccgaa tctgcagggc gtggtgttag aaggggcgaa
     2221 tatcggctta agcacagaaa acgaccgcca gattcgccgg caaaatgacc gccattgggc
     2281 aaaccgtttc cgtcaagaac ccatcgaaca agtgcttgaa gattggtaca aacagcctgt
     2341 ttttgcggat ttaagcgata cggaaagaga atgttatatt gatagcagaa agcacaatca
     2401 aggagagcaa attgctcaaa tgctggaagc caccagcctc gccaaacagc ctgactacac
     2461 cgaaaaactc aagcagacgg ataagaatat tgtctttttt atcggtgaaa aggatcagaa
     2521 atttcgcaaa atggcatcaa gccaccaact accaacacaa ctgattgcta atgcagggca
     2581 taacgctcat cgggctaatg ctaaaaattt tgtggagaaa ttggggttat tgatactcca
     2641 ctccgacagc gacctacggt cgcaatcggt taagcataac ggtgcagctc tgctgcacaa
     2701 aagccccagt ggggcttaat cagccgtagg ctaaatataa cctagtacgg ctcagccgtg
     2761 ctaggtgtta gggctccccc tgcaaccgtt gcaataagcg atccatcgcc cgataaccaa
     2821 gtgcttccgc taaatgcagt tgttgaatat tcgcctcgcc ggctaaatca gcaatagtcc
     2881 gagaaacttt caaaatacgg tggtaggctc gtaccgataa accgagcttt gtcaatgcag
     2941 tttctaaaaa tagggcatct tgtgccgata attggcaatc tcgctcaatt tctcgggtgg
     3001 tgagttgaga attgatttta cctgctcgct caaattgcat ttttctggct aaaaacactc
     3061 gcttgcgaac ctgctcggtg gtctctcctc gattctccgt cccactttgc aatgctcctt
     3121 gtggcaaaag tggcacttct atagagagat cgaaacggtc taagaaaggt ccggaaaggc
     3181 ggtttaaata ccgcattacc tgttggggcg aagtgcggtt atgcgtgcct tgataatgcc
     3241 ctgtcgggct tgggttcatc gctgcaatta attgaaagcg tgccggaaat tgtactttag
     3301 cgttggctcg ggaaatcaca atttcgcctg cctccaacgg ctgacgtaac gcatcaagca
     3361 ctttacgttc aaattcaggc aattcatcta agaacagcac accgttatgg gcaagtgaaa
     3421 tttcccccgg tttggggatc gagccactgt aaaaactaga aataaatgtc ataaaaacta
     3481 gaaatagctg tcagattatt tagaaaacaa gaaataggtg tcaaattatt atgagaaaaa
     3541 cacatagttt tcaacaggtt agaaaaatta aacccacttg gatgagtgta tcggggcata
     3601 ttccatttag aaacggagtc agtattccct atgaatcgac tttagagcgt gattttttga
     3661 tgtatttcac ttatctacct tcagtagatg aagtcgtctc tcaaccgact accctacctt
     3721 tcgtcaagaa tgggatcact tatacctata caccagattt cttcttgtca tttacagacg
     3781 gtcgaaagcc gatgttaatc gaggtcaaac cgaaagcaaa atggcagaag cactggaaag
     3841 aatggaaaga aaaatggaaa gctgcaatac gtttttgcca agaaaacggt tatgtattcc
     3901 atgtgtatga tgaagatcgg attcgacatc tagcgctttt caatctcaac tatgttcagc
     3961 gctataagcg aatacaacat gaacaagaag atatagatgc tattctagcc caagtaaagc
     4021 tgatgggaaa taccactatt gactatttac tctctcgatt ttttgcaggt tctctatatc
     4081 gcatgaaagg cctacaaatc atctatcact tactggcaac gaaacaacta cattgcaatt
     4141 ggtttctacc acttaatgaa tttaccgagg tatgggggaa taatgatgag taactctgga
     4201 tttacagaaa agcgctatca tcaccgatta gatcggggac gtattatttt gcaaaaaggt
     4261 aatatttacc taaatagaga agatggagaa cagtatgaat tggttgatta tatggatgaa
     4321 ccctcccaac tgctcgtacg aaatctcaac actcgtacaa ccaaagtcgt cagtattcat
     4381 cagcttgaaa atttcaaaat gaacgagcga acggatcttt ctgtggatgt aaccgctatt
     4441 agtaatgaat attgggagaa agcccaacaa aaatatgagg caattaaacc gttattaggg
     4501 atggatcaac atcggcctta cgcagttaag gctcgtgcag aagatgttgg ggtgaaccct
     4561 cgtacattat accgttggtt acaagcctat aattccatag gatctatcgc aggtttagtc
     4621 gatcaaaaac gtggatggca acaaggaaat tcccgtttaa cgccagagca agacaagctg
     4681 atcgtgcagg taatcaatga gttttaccta cataaacaac gcccaactac tgagcaaact
     4741 attagagaaa ttcgccgccg atgcaaaatt gaaaaagtgg aaagcccaag caaagaaacc
     4801 atccgtatgc gcattttgca tatttcagag gaagagcgat tacggaaacg cgggcaacga
     4861 gaaaaggcac gcaacaaatt taagccgaag ccaaatagct ttcctgatgc ggattatcca
     4921 ttgagtgtgg tacaaattga ccatacacca gttgacctaa ttattgtgga tagtaagtat
     4981 cgtaaaccga ttggccgacc gtttttgacg gttgcgatag atatatacag cagaatgatt
     5041 gttggttatt acctttcact tgatgcgcct tctgtaactt cggtggcgat gtgtattgca
     5101 cgtggtattt tgccgaaaga acgcctactg cttgatttag gtttgcaagg ctcagaatgg
     5161 aatgcttttg gctacccagt gaaagtgcat gtggataatg gcccagattt tcaggctttg
     5221 gatttatcta aatcttgttc tgcacacgga atccatcttg aatttagacc gatggggcga
     5281 cctgaatatg gcggacatat tgagcgtgtt atcggtacat ttatgaaaga agttcacagt
     5341 ttagcaggaa caacattctc caacatcaaa gagcgtgact cttacgattc ggaaaaagag
     5401 gccattatga ctctcgatga gtttgaaaaa tggttggtac attacattgt gaatgtttac
     5461 cacaaacgtg tgcattctgc tttaggcatt tctcctgaac aaaaatggaa gattggtatt
     5521 tttggagatg aaaatgaagt gggatgtggt tatccgcaac ttcctgttga tgaacaaaca
     5581 ctactacttg atttcttgcc aagtatcact cgtaccattc agcataatgg tgtaacaatt
     5641 gacggacttc gttattacga tgttgcgcta aatatgtata tcagtgattc agatgaaagc
     5701 ggtaaatcta aggaatttct gtttcgccga gacccgcgaa atatcagcaa aatttggttt
     5761 tatgatccga aattaaaacg ttacttccca attccatttg caaatcaggc aatgcccgaa
     5821 atgagcattt gggaatatag agaggttcgt agccgaattg cgaataaagg tgataaatat
     5881 atcaacgagc aacaagtttt agacggttta accgagatgc gtgaaatggt tgctgaatcg
     5941 gctcaacgga cgaaaaaagc acgccgtcag gctgaacgac aaaaaatgca caaagcaagt
     6001 aagccaatta ttgaaacaaa agtggaaact aaggctgttg taccagtcgt agtaacatcg
     6061 aatttgcttg ctttagatga tgagagctta tcttttggtg aggtggacta atgcaatacg
     6121 aacatgttca tgaaaaattc cgccatcttg tgacagcgga caatcaggag aggattgcgt
     6181 tcttggacga gccaagatgg ttaggttatg gtgtcgccaa agacattatg gataatttgg
     6241 tttctttaat gaataaacca aaacgcccac gaatgttgaa tttgttgatt gtcggcgatt
     6301 ccaataatgg aaaaaccaca ctgatccgcc gcttcttcga cttgtacggt caggcgtata
     6361 tcgacagtga ttctaatgcg atttacccta ttcttcttgc cgaagcccca ccaagtgcga
     6421 atgaaaaaga attgtacatc tcattattag agcggttcta cgtaccatat aagccgacag
     6481 atacgatagc caaacttcgt tatcagacta tccatttgtt ccgagaattt cgtgtcaaaa
     6541 tgttggttat tgacgagttt cattctctgt tggttggtac accacgctta caacgacagg
     6601 tgatgaacgc aattaagatg ctttgtaacg agctacaaat tccaattgtc ggtgttggta
     6661 ctcgtgatgc aattcgggta ttacatacgg atcgacaaca tgccagtcgt tttgatgttg
     6721 cggaattgcc aatatggaaa ctagataagg aatttcaaaa actcctattt caattccaag
     6781 ggattttacc tttgagggag agttcaaatc tgcaatctcc agaacttgct acgaagatcc
     6841 acaccatttc aggtgggaac ttagggaacg tgcatcgctt actaacagct tgtgcagttc
     6901 aagccattac aagtggcacg gaaaaaatca ccttagacat cattgagaaa aattcatggg
     6961 tacaaccaac aaacggctat cgcaaaataa tcggataaaa aaaggtgtta ttcatacccc
     7021 tatttattca aatgaaagta ttagctcgtg gttaattcgt gcatcctttg attgtggtac
     7081 agatccactg acttttacag ggttttattg ggaaaaacat cggttgtgga cattagattt
     7141 agatcgtggc ttggaaaata ttgagccaac tatctatgat gatgtacagg cattatcggg
     7201 taaaatccaa ctttcagaat attccttgtt tcgcaggctt taccctataa atgcagatga
     7261 aaccttagct aaaggtcaag taagatgggt gattccacgt tcggcaagga atagaaccca
     7321 tcacattggt caacaatatt gtcctttgtg cttggatgag tcgccacatt tacgcaatga
     7381 ttggcggtta gcgtggaatt ttggttgtct caagcataat atgatgttgg cgaacagatg
     7441 tttttgttgt ggtacattgt atcaacccca tttactatca gcggaaaaga ggcatttgaa
     7501 tcattgtcat caatgtggtg ggaaactgga acaaggcaca atggaaatga gtctagctga
     7561 aaagctgaca ttgcaaggta ttaatcaggt actcgaaaaa gattcaggca tctgttttgg
     7621 atacaaggtg tcgggtaagg tgtggtttga tatgctccgc tactgcatca acttattgag
     7681 acgtgcatca gacagaccaa atcatgcctt agcaaaattt gtagttaatc tacagattga
     7741 tgttgaaaat atatgtagcc ccaaaacagg actttctttt gaacaacttc cgctaggtga
     7801 acgtacaaat ttacttgtga atgcctatca tattttggga atggaagcag attctattcg
     7861 tagtgcaatt tatttgtgtg gcggaacaca aaaaaccttt atgtttgaaa aatatcctga
     7921 tttttggcaa gaatttatgg cactagcacc cgcaggccca aatagaacac gaaaacgcac
     7981 caagccccaa aatgagtcta tcacagcaat taatcgccgt tgggagcgtt tgaaacgcaa
     8041 actacactta aataacgaat gaaaattaat gtaataaaac cacaggaaga accttgctcc
     8101 gcttgtggca aaatttctcg catttggcgc agatataatg gcgaaggcta ctgtgccaat
     8161 tgctatgcta agtggtttcc gaaaaaagag tgcagctgtt gccatcaacc aaagcgtatc
     8221 tatttgagtt caacagtatg ttgggattgc gaaaaacaga ctgactgtgt acgttgcggt
     8281 aaggctgcgg gtacattcaa aattggtaaa attacccgtt atggtgcagt ttgtaatgtg
     8341 tgttcacgtt attttcgtga agaacgagaa tgttttgagt gtggtcaact atctagaact
     8401 atgcttcgag catcagagtc aggtcatagc gagctactct gcttgaagtg ttttcaaaaa
     8461 tatcggtttg cgacttgtaa aaactgtcgc cgttatcgca aagttcatga ccgtgataat
     8521 cagctttgtc gaaaatgtag tgagcaacga acttcacaat gcccgaagtg caaacaggat
     8581 atgcctgcgg gatatggtaa tatttgtgca gattgttcta ggcgaactct actgttcaac
     8641 cttattcgaa tgaatgtgca tttattccgc cacaaaaaag tgaaaagagc atataaaaag
     8701 ttcatttttt ggtatctcgc aaagtgtggc gtgagtgttg tgctgaaaaa atcagacgac
     8761 cacgtcctct tttttatgcg ttgcgatgaa aattggggca acattccaga ctaccgtgag
     8821 ttggttacat tctttaaacc caatggatta cggacgcatt tgactgtgtt gcgttggttg
     8881 cttgatacca atcaagtcaa agtagatgag agcttaaaag aggaaatggc ggggttggaa
     8941 cgtattcaat ccctatttaa caaattaagt aactctgtac cttgtattgc aaactactac
     9001 acgcagttac aacataaata tgaggcgggc aaaacatcac tgaaatctgt tcgattagct
     9061 ttacaacctg cgattgattt catcagttca gaatctgtct cgttactgcc gactcaagta
     9121 caactgaata gctatcttgt ggcaaaaacg ggacagattg ctgctattac aggctttata
     9181 aaccacctga accgagaata tcattgtgaa ttagaaattg accgctccct cattcaaaag
     9241 atgaaagaaa agcagttgaa gcagcaagca aaacagcgat tagcgatact ctacaagcaa
     9301 gaaaaactaa gtatcaaaga gcaaaatgag ctactctcag tcgtattact gaatttgcac
     9361 aacatttgga taaaatcacc gaaaaaatta agtatttcag agatagacgg tatagcctac
     9421 tatgttgata atgagaaaaa atactttata ccaaaagaca ttcacatacg cctgatggaa
     9481 taagctgtcg tcaattttta tttttgcgat ctgtgtcgag aaaataatat ttatgaatcg
     9541 acagatgaaa taatattgat aaagtctctt tgtgtgttca acaaacagga gataaatatg
     9601 acattaaagg ctcaatttcg tgaatggctt aaaaaagagc aagaacatga tgaaatgata
     9661 gggttcgccc atatcccttg cattttatct aaagtggata gcaaactggc gattgttagc
     9721 ggaaatgctt atgagaagaa atatctcaga tattggatca agtgttttag gttcgataga
     9781 aatatcagca agtatattaa ataggttaaa tatctagct
//
LOCUS       DANLXF010000084         9612 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.084, whole genome shotgun sequence.
ACCESSION   DANLXF010000084 DANLXF010000000
VERSION     DANLXF010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9612
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.084"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            101..1579
                     /gene="fruB"
                     /locus_tag="PXD66_001324"
     CDS             101..1579
                     /gene="fruB"
                     /locus_tag="PXD66_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HDL6319621.1"
                     /translation="MLNLSAKNIRLNGSASNKEEVIRFVAAELVAKGNVAAGYEVGML
                     NRETQTSTFLGNGIAIPHGTLDTRDLVKETGVQIIQLPQGVEWGEGNTAYVVIGIAAK
                     SDEHLTLLRQLTTVLSDEEAAATLAKTQNLDEFIAVLSGKKNLPALSQELISLNIESS
                     SPITLSAINAAKLQEQGYVSQAFISDVVANKALHLGNNLFVVDSAKGNQANGIAIARN
                     TQGQTLLTVAETDNALQPLLTQLLKPEVRSQFAHFSAEQMLGLLKGESVASTPVTNVE
                     ASGNQVVGTFTIRNDNGLHARPAAVLVQTVKPFSSKISVENLDRNTAPASAKSAMKVV
                     ALGASQGHRLRFVAEGEDAQQAIEALQQAIINGLGESVSFVPAVADTIESIAVSAIPA
                     EETATAVDANTKEATFVIKNEHGLHARPSAVLVNEVKKFNAKIEVQNVDKNSPLVSAK
                     SLMKIVALGVTKGTTLRFVASGDDAEVALAAIGAAIEAGLGE"
     gene            1593..2537
                     /gene="fruK"
                     /locus_tag="PXD66_001325"
     CDS             1593..2537
                     /gene="fruK"
                     /locus_tag="PXD66_001325"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDL6319622.1"
                     /translation="MRIATVTLNPAFDLVGKLQRIEIGEVNTVETLGLYPAGKGINVA
                     KVLADLGTKLSVTGFLGEENQGDFVNAFKHNDLADEFYRINGKTRINVKITETEADVT
                     DLNFLGFEISESDWEKFVTLSHQWESKFDLVAVCGSLPRGVTPEKFASWLESLHRQGL
                     KVVLDTSNAALTAGLTAHPWLVKPNRRELEVLVGHSLNSIEEIISAAKQLRSRGIENV
                     IISMGEQGSLWLNHEGVLQARPPRCENVVSTVGAGDSMVASLIYGLSQGWSKEQTLRF
                     ASATSALAVVQSNVGIADRAALDDIIKQTDITVISENH"
     gene            2563..4227
                     /locus_tag="PXD66_001326"
     CDS             2563..4227
                     /locus_tag="PXD66_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL6319623.1"
                     /translation="MNISFVFPQTLGNARRFLVNEMLSAAAKQQGHNVVAANQADFVV
                     LFDNAIPQEAAGKQGAILDLEQAFAQPEASLKQAVENAQTFANATATTAVSAVSSGVK
                     NIVAVTACPTGVAHTFMSAEAIENYAKAQGWNIKVETRGQVGAGNPISAEEVAAADLV
                     FVAADIDVDLEKFKGKPMYRTSTGLALKKTTQEFEKAFAQAKIYEGGVAKADNAEAPT
                     SEKKGLYKHLMTGVSHMLPLVVAGGLLIAISFMFGIEAFKDETIAGGLPKALMDIGGG
                     AAFHLMIAVFAGYVAFSIADRPGLAVGLIGGMLATTAGAGILGGIVAGFLAGYTVKFL
                     NGAIQLPASLTSLKPILILPLLGSAIVGLLMIYVINPPVKALMDALVEWLNTMGQTNA
                     IILGIILGAMMCTDMGGPVNKAAYTFGVGLIASQQYMPMAAVMAAGMVPPIGMAIATL
                     IARSKFNTNQRDAGKASFVLGLCFISEGALPFVAADPVRVIASSILGGATAGAISMAL
                     GITLQAPHGGLFVIPFVSQPLMYLAAIAIGSLVTGVVYAAIKQKAE"
     gene            4394..4912
                     /locus_tag="PXD66_001327"
     CDS             4394..4912
                     /locus_tag="PXD66_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lhr family helicase"
                     /protein_id="HDL6319624.1"
                     /translation="MILLRGDTMQFIEFTDPDSGISYQYSEFTIANVAFIINFCSDAD
                     VISTLSALGKDITNYINTYSCCTIKFMAKEHLENSGSNIDIYAPAANHQFKRKEIIAL
                     QETLERLLFEHYVRFTPESYLFIAERDSLNRMYQRMCVPRCDFMQSFQVVYPLGVNQD
                     CFILITPKGNLK"
     gene            complement(5065..5928)
                     /gene="rarD"
                     /locus_tag="PXD66_001328"
     CDS             complement(5065..5928)
                     /gene="rarD"
                     /locus_tag="PXD66_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDL6319625.1"
                     /translation="MLKGILASLAANVLFGAVYYFAILLRPLEGEAMFGFRIVVVIPF
                     ILLAIILFKQQSAFADLLQKIRQNPPLVLVILLLALNTGVQLWLFLWAPNHGQALQVS
                     FGYLLLPIVAVALGKMVFKEHFTRLKWLSLLFAVIGVGSSLFFNSTISWATWVAGLGY
                     PLYITLRRYFQINTLATFFVELLLVLPVALYYLSQVEMTPIVAENPQIYFYLGLLGLV
                     SGSAFILYILSSNLLPINVLGLLGYVEPLVMLLISFAIGEKLDANSYILMVCLLVSIG
                     LLSWDSFRSKV"
     gene            6106..6690
                     /locus_tag="PXD66_001329"
     CDS             6106..6690
                     /locus_tag="PXD66_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HDL6319626.1"
                     /translation="MKKIFWILLIAMLAVGGYSLTKYNDLMRAEEDINSVWGNVESAY
                     QRRADLIPNLVNTVKGQANFEKETLTSVIEARAKATAVTIDPSNATEEQMAKFQEAQQ
                     GVNSALSRLLVSVEKYPELKAHEAFLNLQAQLEGTENRINVERNNFNEKVKEYNKQVR
                     EFPTKLAAMIMGFKAKPQFKSEAGSDKAPTVNFN"
     gene            6772..7230
                     /locus_tag="PXD66_001330"
     CDS             6772..7230
                     /locus_tag="PXD66_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDL6319627.1"
                     /translation="MGLFSFLSNKLPIDTQRIEQAIADLEQKTSAELRVVVERKAKIG
                     KTDNAAMLRASELFDELKMRETAEHNGVLIYLSFKPHYAAIIGDEGIHQKVDNDFWQK
                     IYATMCLDCQQGDYTQAICDAIKMVEAPLAHHFPYQADDKNELSNEVVVK"
     gene            7227..8084
                     /locus_tag="PXD66_001331"
     CDS             7227..8084
                     /locus_tag="PXD66_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDL6319628.1"
                     /translation="MMKSLCKFLSIFNRLLLVCLALFSLNATAVTYPEPPNPFYYITD
                     YTKNTLSQQEWRTLEDALIANCAKTSSQIAVVIIPSTEGEAVSTYAHTLFNKWGIGRS
                     KNNENNGILLLIAKNDRKLFIATGRGLEGALPDAIASSIIRNDITPFFKQEQYAAGIA
                     HGLSAIMAAINGEYAPYASYGEEMQEFDDIDGLLFFLFAGIVIFLIFRPRGNSTYISP
                     SSMDQLGQILRESQRRGGGFGGGLGGGFDRGSGGFGGGFGGGFGGSRGSGDSFGGGSS
                     GGGGAGGSW"
     gene            complement(8199..8678)
                     /gene="ispF"
                     /locus_tag="PXD66_001332"
     CDS             complement(8199..8678)
                     /gene="ispF"
                     /locus_tag="PXD66_001332"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HDL6319629.1"
                     /translation="MIRIGHGFDVHAFGEDRPLMIAGVEVPYHTGFIAHSDGDVALHA
                     LTDALLGAAALGDIGKLFPDTDMQYKNADSRGLLIEAYRQVQEQGYQVGNVDVTIIAQ
                     APKMRPHIDKMRQVIADDLQCDISQVNVKATTTEKLGFTGRGEGIACEAVALLLKRA"
     gene            complement(8680..9363)
                     /gene="ispD"
                     /locus_tag="PXD66_001333"
     CDS             complement(8680..9363)
                     /gene="ispD"
                     /locus_tag="PXD66_001333"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDL6319630.1"
                     /translation="MKRKIIAVIPASGVGSRMQAEKPKQYLQLKGKTILEHTVSIFLE
                     HPHVEKVIVAVARKDPFYSTLELLKSPKIQLVFGGETRADSVLNALQEINTENAWALV
                     HDAARPCLKRSDLDKLLQINDPQGGILAIPAVDTMKRANGTKIAHTEDRSTLWHALTP
                     QFFPAQALKQALSSAFANKLQVTDEASAMELAGYQPQLVSGRSDNIKITRPEDLALAE
                     FYLTHNLEN"
     gene            complement(9381..>9612)
                     /gene="ftsB"
                     /locus_tag="PXD66_001334"
     CDS             complement(9381..>9612)
                     /gene="ftsB"
                     /locus_tag="PXD66_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HDL6319631.1"
                     /translation="YSFWFGKNGWSDYQEVQEEIALLKVENTKLTSRNKLIEAEIYDL
                     KNGVDALEERARLEREMVKENEMFYRIVPKNK"
BASE COUNT         2876 a         2078 c         2013 g         2645 t
ORIGIN      
        1 tatggatatt tttagttggc tataacggtt tagcaaaata tagagtaaac taaccataac
       61 attacagttt aaaaatcaac tttaaatcca aggagctaaa atgcttaatt tatcggcaaa
      121 aaacatccgt ttaaatggtt cagcaagcaa caaagaagaa gttatccgtt ttgttgccgc
      181 cgaattagtc gccaagggca atgtagcagc cggctatgaa gtcggtatgc ttaatcgtga
      241 aacccaaacc tctacttttt taggtaacgg aattgctatt ccccacggca cgcttgatac
      301 ccgtgattta gtcaaagaaa ccggcgtgca aattatccaa cttccgcaag gcgtggaatg
      361 gggcgaaggc aacactgctt atgtagttat cgggattgcg gctaaatcgg acgaacactt
      421 aaccttactc cgtcaactca ccactgtatt aagtgatgaa gaggctgctg ccacattagc
      481 gaaaacgcaa aatttagatg aatttattgc cgtattaagc ggtaagaaaa accttcctgc
      541 cttatctcaa gagctgattt ctctgaatat cgaatcttca agccctatca ctttatcagc
      601 cattaacgcc gcaaaactac aagagcaagg ctatgtttcc caagcattca tcagcgatgt
      661 ggttgccaac aaagcgttac atttaggcaa taatctattt gtggtggatt ccgccaaagg
      721 caaccaagct aacggtatcg ctattgcccg taatacgcaa ggtcaaaccc tactaactgt
      781 ggcagaaacc gacaatgctc tacagccact attaactcaa ttattaaaac cagaagtaag
      841 aagccagttt gctcatttct ctgcggagca aatgcttggt ttattaaaag gcgaaagcgt
      901 agcttcaacc cctgtaacca atgtcgaagc gagtggcaac caagtggttg gcacattcac
      961 cattcgtaac gacaacggct tacacgcccg cccggctgcg gtgttagtac aaacggtgaa
     1021 accgttctcc tcaaaaatca gcgttgaaaa tttagaccgc aacactgcac cggcaagtgc
     1081 gaaaagtgca atgaaagtag tagcactagg ggcaagccaa gggcatcgtt tacgctttgt
     1141 ggcagaggga gaagacgccc aacaagcgat tgaggcatta caacaagcaa ttattaacgg
     1201 cttaggcgaa agtgtttcat tcgtaccggc ggtggcagat acgattgaaa gcattgctgt
     1261 ttcagcaatt cctgcggaag aaactgcgac tgctgtcgat gccaatacga aagaagcaac
     1321 ttttgtaatt aaaaacgaac acggtttaca cgctcgccca agtgcggtgt tggtgaatga
     1381 agtgaaaaaa ttcaacgcca aaattgaagt gcaaaatgtt gataaaaatt caccgcttgt
     1441 aagtgccaaa agcttaatga aaattgttgc ccttggcgta accaaaggca cgacgctacg
     1501 ctttgtggca agcggtgatg atgccgaagt agcacttgcg gcaatcggtg ctgcgattga
     1561 agcaggttta ggcgaataag cgaggaatca ttatgcgtat tgcaaccgtt accctcaacc
     1621 ctgcctttga tttagtcggc aaacttcaac gtattgaaat cggcgaagtg aacaccgtag
     1681 aaacccttgg tttataccct gccggcaaag gtattaacgt tgctaaagtt ttagctgact
     1741 taggcacaaa actttcggtt accggctttt taggcgaaga aaatcaaggc gatttcgtaa
     1801 atgcctttaa acacaatgat ttggctgatg aattttaccg tattaacggt aaaacccgca
     1861 ttaatgtcaa aatcactgaa accgaggctg atgtaactga tctgaacttt ctcggctttg
     1921 aaatttcgga aagcgattgg gaaaaattcg tcacattatc tcatcagtgg gaaagcaaat
     1981 ttgacttagt tgcagtgtgt ggatctctcc ctcgtggcgt aacgccggaa aaatttgctt
     2041 cttggttgga atctttacac cgccaaggct taaaagtggt gttagatacc agcaatgcgg
     2101 ctctcaccgc cggcttaacc gctcaccctt ggttagtaaa accaaaccgc cgagagctgg
     2161 aagtgctggt cgggcattca ctcaactcaa tcgaagagat tattagtgca gctaaacaat
     2221 tacgttcccg tggtattgaa aatgtaatta tatcaatggg cgaacaaggc tctctttggc
     2281 taaatcacga aggcgtgtta caagcccgcc caccacgttg cgaaaatgta gttagcactg
     2341 taggtgctgg tgattcaatg gttgccagct tgatctacgg cttatctcaa ggttggagca
     2401 aagagcagac cttgcgtttt gcctcagcta cttctgctct agctgttgtt caaagtaacg
     2461 ttgggattgc agaccgtgcg gcgttagacg atattattaa acaaaccgac attaccgtga
     2521 tttcagaaaa tcattaaacc tcaatcaaag gaacaaacca atatgaatat ttcttttgta
     2581 tttcctcaaa cactaggcaa cgcacgccgt ttcttagtga atgaaatgtt atctgccgct
     2641 gccaagcaac aaggacacaa tgttgtggcg gcaaaccaag ctgattttgt ggtgttattc
     2701 gataacgcaa ttccacaaga agctgccggc aagcaagggg caattttgga cttagaacaa
     2761 gcctttgctc aacctgaagc cagtctcaaa caagcggtcg aaaatgcaca aacttttgca
     2821 aatgctaccg caaccactgc ggtttcagcg gtttcatctg gtgtgaaaaa tatcgttgct
     2881 gttaccgctt gcccaactgg cgtggctcat acttttatgt ctgcggaagc aatcgaaaat
     2941 tatgccaaag cacagggttg gaacatcaag gttgaaaccc gtggtcaagt aggagcagga
     3001 aatccaattt ccgccgaaga agtcgcagcg gcagatttag tctttgtagc tgccgatatt
     3061 gatgtcgatt tggaaaaatt caaaggcaag ccgatgtatc gcacctcaac cggtttagca
     3121 ttgaagaaaa ccactcaaga atttgaaaaa gcctttgctc aagcgaaaat ttacgaaggt
     3181 ggtgtggcaa aagcggataa tgctgaagct ccaacttctg agaaaaaagg gttatataaa
     3241 cacttgatga ccggtgtatc acatatgtta ccgctggtgg tagcaggtgg cttgctcatc
     3301 gctatttcct ttatgttcgg aattgaagcc tttaaagatg aaaccatcgc aggcggcttg
     3361 ccgaaagcct taatggatat tggtggcggt gcggcattcc acttaatgat tgcggtattc
     3421 gcaggctatg tagcgttctc aattgcagac cgtccggggt tagctgtcgg tcttatcggc
     3481 ggtatgcttg ctaccaccgc aggagcaggg attttaggtg gtatcgtagc cggtttctta
     3541 gccggttata ccgtaaaatt cttaaatggt gcgattcagc taccggcaag tttaacctca
     3601 cttaaaccta ttttgatttt accactctta ggctcagcga ttgtcggctt actgatgatt
     3661 tatgtcatca acccaccggt gaaagcatta atggatgcct tagtggaatg gttaaacaca
     3721 atggggcaaa ccaatgcaat tattttaggt attattctcg gtgcgatgat gtgtaccgat
     3781 atgggtggtc cggtgaataa agcggcttat acctttggtg tgggtttaat tgcttcacaa
     3841 caatatatgc caatggcagc ggtgatggcg gcaggtatgg ttccaccaat tggtatggcg
     3901 attgcgaccc tgattgctcg tagcaaattc aacaccaacc aacgtgatgc aggtaaagct
     3961 tcattcgtgt taggtttatg ctttatttca gaaggggcat taccgttcgt ggcggcagac
     4021 cctgtgcgtg taattgccag ctcaatctta ggcggtgcaa ccgccggtgc aatttcaatg
     4081 gcactaggca ttaccctaca agccccacac ggcggtttat tcgtgattcc attcgtttca
     4141 caaccgttaa tgtacttagc tgctattgcc atcggctcat tagtcaccgg cgtagtctat
     4201 gccgctatca aacaaaaagc agaataaagc acaaacacaa atacaaacaa aaacggagcg
     4261 aaacgctccg tttttatatc atattggtac ttactgaatt ctatttcatc gaaatatcaa
     4321 gtctaaaata ttatttttgc gatcgagatc gaaaaaatca ttttcatcac tttacgaata
     4381 atatcaactc gttatgattt tgctacgagg agatactatg cagtttattg aatttaccga
     4441 tcctgatagt ggaatttctt atcaatacag tgaatttact attgctaatg tcgcatttat
     4501 cattaatttc tgctcagatg ccgatgttat atcgactttg tccgcattag gcaaagatat
     4561 cacaaactat ataaacacct attcctgttg cacaatcaaa tttatggcga aagagcatct
     4621 agaaaatagc ggttctaata ttgacattta tgcccccgca gcgaatcatc agttcaaacg
     4681 taaagaaata attgctttac aagaaaccct tgagcgttta ttatttgagc attatgtacg
     4741 ttttacacca gaaagctatc tcttcattgc cgagagagat tcacttaacc gaatgtatca
     4801 gcgtatgtgt gtgccacgat gtgattttat gcaatctttc caagtggttt atcccttggg
     4861 cgttaatcaa gattgtttca ttttaattac gccaaaagga aatctaaaat gaatcaacaa
     4921 cgtccagttt ccgttcgttt aaagcaagaa ggcttaatcg cttttaagca agcctatcag
     4981 cgagcaaaaa aagcaggtct attgaaaccg gtcaaataat ctttaaaccg cttgctctac
     5041 aagcgattta tttttcctta attatcacac cttgctacga aaactatccc aactcaataa
     5101 tccaatcgaa accagcaaac agaccatcaa aatataggaa ttggcatcga gtttttcgcc
     5161 gatggcaaaa gaaatcagca acatcaccaa cggttcaaca tagcccagta aacctaatac
     5221 attaatcggt aataaattgc tcgagaggat ataaagaata aaagcactgc cacttaccag
     5281 ccccagcaat ccgaggtaga aataaatttg cggattttct gccactattg gcgtcatttc
     5341 cacttggctg agataataaa gtgcaacggg taataccagt aacaactcca caaaaaaagt
     5401 agctaacgtg ttgatttgaa aataacggcg aagggtaata taaagcggat aacccaaccc
     5461 agctacccaa gtcgcccacg aaatcgtgct gttaaaaaac aaactactgc caacaccgat
     5521 gactgcgaaa agtagagaaa gccatttcag gcgggtaaaa tgttctttaa acaccatttt
     5581 tcctagagcc accgccacaa tcggtaaaag caagtaacca aacgacacct gcaacgcctg
     5641 cccgtgatta ggagcccata aaaacagcca aagctgcacg ccggtgttga gagcaaggag
     5701 caaaatgacc aatacaagcg gtggattttg tcgaattttt tgcaaaagat ccgcaaaagc
     5761 cgattgctgt ttaaacaata taatcgccag caaaataaac ggaatgacta ccactatccg
     5821 aaagccgaac atcgcctctc cctctaacgg gcgaagcaat atcgcaaaat agtaaactgc
     5881 tccaaacagc acattggctg caagcgaagc taaaattcct ttgagcattt ggctttcctc
     5941 ttatcaacaa tcaaacaaca agcggttgac tttgaacaaa aatttacaaa tcttatctac
     6001 gccccatagt atgaaaaaat ttgtaagtaa gctagctata ttcgattttt cccgatacaa
     6061 tgctcccaat ttttcactct tttatttata ttataggact tcataatgaa aaagattttt
     6121 tggattttac tgatcgccat gcttgctgtt ggcggttatt ctttaacaaa atacaacgat
     6181 ttaatgcgtg cagaagaaga catcaactcc gtatggggaa atgttgaatc ggcttaccaa
     6241 cgccgtgcgg atttaattcc aaacttagtg aataccgtaa aaggtcaggc taactttgag
     6301 aaagaaactt taacctctgt gattgaagct cgtgcaaaag caactgcggt aaccattgac
     6361 ccaagcaatg caactgaaga gcaaatggct aaattccaag aagcccaaca aggtgtaaac
     6421 tctgctcttt cacgcttatt ggtttcagtt gagaaatacc ctgaattaaa agcccacgaa
     6481 gcgtttttaa atctacaagc tcaattagaa ggtacggaaa accgcattaa cgttgaacgc
     6541 aataacttta acgaaaaagt gaaagagtac aacaagcaag tgcgtgaatt cccgactaaa
     6601 ttagcggcaa tgattatggg ctttaaagca aaaccgcaat ttaaatctga agcaggttct
     6661 gataaagcac caaccgtaaa cttcaactaa ttttagtttc aatttattca tattaaaagc
     6721 aataagctct ttcaaatccg gtgcttattg ctttattttt aaggggaaca aatgggatta
     6781 ttttcttttc tctccaacaa attaccgata gatacgcaac gcattgaaca agcaattgcc
     6841 gatttagagc aaaaaacctc tgccgaattg cgtgtggtgg tggaacgaaa agccaaaatc
     6901 ggcaaaaccg acaacgccgc tatgttgcga gcaagtgagc ttttcgatga attaaaaatg
     6961 cgtgaaacgg ctgaacataa cggtgtgttg atctaccttt cattcaagcc tcactacgct
     7021 gcgattattg gcgatgaagg cattcatcaa aaagtcgata acgatttttg gcaaaaaatc
     7081 tatgccacaa tgtgtttgga ctgccaacaa ggcgattaca cccaagccat ttgtgatgca
     7141 atcaaaatgg tggaagctcc gctcgctcac catttccctt atcaggctga tgataaaaac
     7201 gagctttcaa acgaggtggt agtcaaatga tgaaatccct ttgcaaattt ctatctattt
     7261 tcaaccgctt gttattggtg tgcttggctc tgtttagcct aaatgccaca gcggtgacct
     7321 acccagagcc gcctaatccg ttttattata ttacggacta caccaaaaac accttgagcc
     7381 aacaagaatg gcggacatta gaagatgcct taattgctaa ctgtgccaag acaagttcgc
     7441 aaattgccgt ggtgattatt ccaagcacgg aaggcgaggc agtttcaacc tatgctcata
     7501 ccctgtttaa caaatgggga atcggacgga gcaaaaataa cgagaacaat ggtatattgt
     7561 tattaattgc caaaaatgat cgcaaactct ttattgccac cggacgtggg cttgaaggag
     7621 cgttacctga tgcgattgca tcgagcatta tccgcaatga catcacgcca ttcttcaaac
     7681 aagagcaata tgcagccggt atcgcccatg gcttatccgc cattatggca gccattaacg
     7741 gggaatatgc cccttatgca agttatggcg aagaaatgca agaatttgat gacatagacg
     7801 gcttactctt tttcttgttt gccggcattg tgattttcct aattttccgc ccacgaggaa
     7861 acagtaccta tattagtccc agctcaatgg atcaactagg acaaatacta agagaaagcc
     7921 aacgcagagg tgggggcttt ggcggtggtc tcggaggtgg ctttgatcga ggctcaggtg
     7981 gatttggcgg aggcttcggt ggtggttttg gcggaagcag aggcagtggc gacagcttcg
     8041 gtggtggttc atcaggcggt ggaggcgctg gaggaagctg gtagtttccg cccaatttaa
     8101 aacaatacaa gcggtcatat ttctcaaaaa aattgcgtat ttttcgctaa acgcacaaaa
     8161 cgctaatgaa aataagaccg cttcctcact tctctaagct atgccctttt tagtaacaat
     8221 gctacggctt cacaagctat gccttcacct cgcccggtaa accctaattt ttctgttgta
     8281 gtggctttta cattcacttg tgatatatcg cattgtagat cgtctgcgat cacctgtctc
     8341 attttatcaa tatgcggtcg catcttcgga gcttgagcaa taatggttac atcgacgtta
     8401 ccgacttgat agccctgttc ttgcacttgt cggtaagctt caatcaacaa accacgacta
     8461 tcggcatttt tgtattgcat atcagtatca gggaataatt tgccaatatc acctaaagct
     8521 gctgcgccaa gcaacgcatc tgttaaagcg tgcagcgcta catctccgtc actatgcgca
     8581 atgaagcctg tatgataagg cacttcaacc ccagcaatca tcagtggtct atcttcgcca
     8641 aaagcgtgaa catcaaaacc gtgtccaata cgtatcattt tagttctcca aattatgtgt
     8701 aaggtaaaat tccgctaagg ctaaatcttc agggcgagtg attttgatat tatcacttct
     8761 acctgaaacc aattgtggct ggtagcctgc aagctccatt gcagatgctt cgtctgttac
     8821 ctgaagctta ttggcaaaag cagaggataa tgcttgtttt aatgcttgag caggaaaaaa
     8881 ttgtggggtt aaagcgtgcc ataaggttga gcgatcttct gtgtgtgcga ttttcgtgcc
     8941 gtttgctctt ttcatcgtat ctaccgctgg aatggcgaga atgccacctt gcggatcgtt
     9001 aatttgcaaa agtttatcta aatctgaccg cttgaggcaa gggcgagccg catcgtgaac
     9061 taatgcccaa gcattttcag tgttgatttc ctgtaaggca tttagcactg aatctgctct
     9121 cgtttctcct ccaaaaacaa gttggatctt aggggatttt aggagttcca atgtggaata
     9181 aaaagggtct tttcttgcca ccgctacaat cactttttca acatgagggt gttcaagaaa
     9241 aatagacact gtatgctcta agatagtctt accctttaat tgtaaatatt gttttggttt
     9301 ttctgcttgc atccggctac caactcctga cgccggaatt actgcaataa tttttcgctt
     9361 cataatatat caattatgta ttatttgttt tttggtacaa ttcggtagaa catctcgttc
     9421 tcttttacca tttctcgttc aagtcttgct ctctcttcaa gtgcatctac gccgttcttt
     9481 aaatcgtaaa tttcggcttc aattagttta ttgcgtgaag taagctttgt gttttctact
     9541 tttaataaag caatttcttc ttgcacttct tgataatcac tccaaccatt ttttccaaac
     9601 caaaagctat at
//
LOCUS       DANLXF010000085         9591 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.085, whole genome shotgun sequence.
ACCESSION   DANLXF010000085 DANLXF010000000
VERSION     DANLXF010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9591
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.085"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            2..595
                     /locus_tag="PXD66_001335"
     CDS             2..595
                     /locus_tag="PXD66_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007524923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HDL6319632.1"
                     /translation="MALSLTALVVSSMSVAGTLSTSSMVDILAFDGQRVKSGTTSVQM
                     SENKTHQVVVEVGGTVEGDFFTSDQIILTFQGSAENAKLETPRLNSKLDLRKFQENQT
                     VAIKTDSGKVISHKQDFLKGEGFLAKTRVEDNLTKYNLAKKTASVEQFATAPLEAKGQ
                     IVVDTNKVSQEELQLLFRKADKDTQKRFLEWAKKNIK"
     gene            complement(676..1059)
                     /locus_tag="PXD66_001336"
     CDS             complement(676..1059)
                     /locus_tag="PXD66_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319633.1"
                     /translation="MKTLKLGCIFSLALMLSSCTLTPEQQAERKAKQIRAEQDLQVQL
                     AKQCDVETAELMYQQFNPPLSQTEQEEKAFKKRYAEKVNAPMFQACYKLAWQNYKAQV
                     ELEEIRWNYDRDYMYRGWRYCYYCW"
     gene            complement(1089..1658)
                     /locus_tag="PXD66_001337"
     CDS             complement(1089..1658)
                     /locus_tag="PXD66_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDL6319634.1"
                     /translation="MNETTINDFSLLCRLFGNLFYRHPTDPILAGTFNWLAQGGLRQV
                     WALNTDSQSENALTLLQKNANPTQLAASYQALFAENGAISPTISAYGLSVNNFVAFRQ
                     ERAMPELENADHIGLLFLTASWIEDNLDSALAQKILFEQFLLPCAGKFLGKIEAFDNG
                     FYKALAQLSRDALSAMADELEESELEAVE"
     gene            1717..2562
                     /gene="nfo"
                     /locus_tag="PXD66_001338"
     CDS             1717..2562
                     /gene="nfo"
                     /locus_tag="PXD66_001338"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDL6319635.1"
                     /translation="MKYIGAHVSASGGVENAVLRSVEIGANAFALFTKNQRQWQAPAL
                     KAETIEKFKRFCKVHHFSAEQILPHDSYLINLGNPEAEALEKSRVAFIDEMSRANQLG
                     LKLLNFHPGAHLKQISEQDCLARIAESINIAVEKVPNVIAVIENTAGQGSNLGWHFDH
                     LATIIEQVEDKSRVGVCLDTCHLFSAGYDISSYEKSEAVFNDFNQTVGFHYLRGMHLN
                     GSKTPLGSRVDRHHTLREGTIGTEVFAFIMQNMHFNHIPMILETISPEIWADEIKFLR
                     TLELK"
     gene            2690..3262
                     /locus_tag="PXD66_001339"
     CDS             2690..3262
                     /locus_tag="PXD66_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL6319636.1"
                     /translation="MKKNLFATTFKPVFIASILSVFGGYSSIAVASLSSKPATPNQII
                     ASKAERLAKSSSVFTNRANLPTSSAHTANYSGRINSVYHNWVGTRYRLGGTTKAGIDC
                     SAFVREVMGKAFSKDMPRSTAEQRHVGHSISKSDLRPGDLVFFRKNNHVGVYIGNGKF
                     VHASSSRGVTTSSLSESYWSRNYTQSRRVI"
     gene            complement(3329..3787)
                     /locus_tag="PXD66_001340"
     CDS             complement(3329..3787)
                     /locus_tag="PXD66_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL6319637.1"
                     /translation="MASIRQISDQTAAYQPIPLYRDFNSSRSVKLDLNSLCIKRPRDT
                     FFIHVKNPNLIAWGIELDDLLIVEQTNQYLVNDLLVLEKDGEYKFYQFFNEIQNGEGI
                     AEKILFSLDVSESNLRITDWAEVKIAGVITNVVHQMRTRGAFTQAKQRVA"
     gene            complement(3961..4344)
                     /gene="grcA"
                     /locus_tag="PXD66_001341"
     CDS             complement(3961..4344)
                     /gene="grcA"
                     /locus_tag="PXD66_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HDL6319638.1"
                     /translation="MIKGVQITESSNSNLLNSFWLLDEAKNEARCLAAKAGFEEDQVV
                     AISELGDIAYREVPVNVAPTIKVEGGQHLNVNVLRRETLEDAIQNPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVITRTFTESL"
     gene            4503..5192
                     /gene="ung"
                     /locus_tag="PXD66_001342"
     CDS             4503..5192
                     /gene="ung"
                     /locus_tag="PXD66_001342"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDL6319639.1"
                     /translation="MKTWTEALGEEKQKAYFQQILQYVHNERLAGKVIYPPQNEVFSA
                     FALTEFSDVKVVILGQDPYHGPNQAHGLAFSVKPPVAPPPSLVNMYKELSEDIEGFQI
                     PPHGYLIDWAKQGVFMLNTSLTVEQGRAGSHSQIGWEIFTDKVIAQLNQHRDNLVFLL
                     WGSHAQRKGQFIDRNRHCVLASPHPSPLSAYRGFFGCKHFSKANNYLISQGISPINWQ
                     LSVDVSYSSTH"
     gene            5208..6128
                     /locus_tag="PXD66_001343"
     CDS             5208..6128
                     /locus_tag="PXD66_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HDL6319640.1"
                     /translation="MNFDLPIVSIAFVVLVLVALSSTIKIVPQGYHWTVERFGRYTKT
                     LSPGLNIVVPFIDRIGRKMNMMEQVLDIPSQEVISRDNASVAIDAVCFVQTVDARRAA
                     YEVNHLEQAIVNLTMTNMRTVLGSMDLDDMLSQRDLINGRLLSIVDEATNIWGVKVTR
                     IEIRDVRPPKELVAAMNAQMKAERNKRADILEAEGIRQAEILRAEGEKQSRILKAEGE
                     RQEAFLQAEARERAAEAEAKATQMVSEAIAKGDTTAINYFIAQKYTEALKEIGSSDNS
                     KVVLMPLEAGNLIGSVAGIAEILKSNKTSS"
     gene            6164..6601
                     /locus_tag="PXD66_001344"
     CDS             6164..6601
                     /locus_tag="PXD66_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDL6319641.1"
                     /translation="MDWFFNWIGWSILGFVLLALELVLPGVFIMWWGFASLILAFIVA
                     LFTLGQALQIALFAIIATIFSLIWWKYQHGKDKKEDQSSSLNSREHAMLGAQGFVVEI
                     LPNGIARGKFGDTTWRVIGNNLQVADKIEVQKVEGITLFVRKQ"
     gene            complement(6742..9546)
                     /locus_tag="PXD66_001345"
     CDS             complement(6742..9546)
                     /locus_tag="PXD66_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDL6319642.1"
                     /translation="MYKIKHSFNKTLIAISISSFLSIAYATESIENPQPIIQLSESLS
                     SKYSGKGVKLGVMDEGFMVKHPRHSSHLHPLIHQLTTPEGEVRIYDASYPQFEVNPVE
                     KEDGIDLIPSLETHGAGVAGIIAAQADKTLGDGYSGGIAKGAELYVATKSYKRTLEKV
                     IQDAKKELENAKDEEDEKTPSLDQMAKNDLLASKEKEMAIERAEWASGLNKLLDNNVF
                     AINNSWNPFSISDDINVVDKFYQSIKQNKHNPLLQAIMRAKNSNTLLVFAAGNESKKQ
                     PGVMALLPRYFPELEKNLISAVAVDKEQKIASYSNHCGASKNWCVAAPGDLHVLIGVA
                     DEHKKPQYGLTKEQGTSFSAPAITASLAVLKERFDYLTATQIRDTLLTTATDLGEKGV
                     DNVYGWGLINLKKAVNGPTQFLNDETITVTRDDHWSNPLASQFKITKKGDKSLHLDGE
                     NHLDTVAVEEGRLALNGKTKVKTISNHANLAVNGTEVEQNYSSSGQSQLEVLGKSGLI
                     ANAQANIHLAGSLKIDDKLTEKTEAGDVSATVVQLKDKATYQGGFTQLVENENLAKRG
                     LIQDLYFKESEIIAKVNKPLTDEKADTNGQAGLALLNALRTTPIAYRRSWYNGWLQSA
                     LEQRKLDNLHYAVSNNIYADSLELLRSQNAKGLTQAQQHLFTAYHTPLQTTVWAEHLN
                     QKQSASSKHTDVKHHQSQLGVNHKLADKTVLSATLSQQKNRLEKPFAQATLKQTALNI
                     GLRYHLDNAWFSEATLQFARQKYQQSRRFASHQLGTAETRGSTLGGEMRIGYQFMPNQ
                     WIIEPSLGVQWIQTKMNGLNESGELATQTAAMRYRDVNIVPSVKLQRTFQLEQGSISP
                     YIGLNYLHRLNGKITKITSNIAGKTLHSEATTKRNRQLNGEVGVKLHYKNWFTAMNLD
                     YSRVKSANLFGWKVNVGFSF"
BASE COUNT         2828 a         1721 c         1919 g         3123 t
ORIGIN      
        1 aatagcatta agtttaacag ccttagttgt cagttcgatg agtgtggccg ggacgttaag
       61 tacatcatca atggtggaca ttttagcctt tgacgggcaa cgtgttaaat ccggcacgac
      121 ttcggttcaa atgagtgaga ataaaacgca tcaagtggta gtggaagtcg gtgggacagt
      181 tgaaggggat tttttcacct ctgatcaaat cattttgact ttccaaggca gtgcggaaaa
      241 tgcaaaatta gaaacgccaa gattaaattc aaagttagat cttcgtaaat tccaagagaa
      301 tcagactgtg gcaattaaaa ccgattcagg taaggttatt tcacataaac aagatttctt
      361 aaaaggggaa ggtttccttg cgaaaacaag agttgaagat aatttaacga aatataactt
      421 agcgaaaaaa acagcttcag ttgaacagtt tgcgacggca cctcttgaag caaaaggaca
      481 aattgtggtg gatacaaata aagtttcaca agaagaatta cagcttttat ttagaaaggc
      541 agataaagac acccaaaaac gtttcttaga atgggcgaag aaaaatataa aataaaccta
      601 tatcaattaa taagcggtca tttctcgcca attttttgca aggtgttaaa aaaatgaccg
      661 ctcatacaat gagatttacc agcaataata gcagtaacgc cagcctctat acatatagtc
      721 tctatcgtaa ttccaacgaa tttcttcaag ttctacttgt gccttgtaat tttgccaagc
      781 aagcttataa caggcttgga acattggggc attgactttt tcggcatagc gtttcttaaa
      841 tgctttctct tcttgttcgg tttggctaag cggcggatta aattgttggt acattaactc
      901 cgccgtttct acatcacatt gtttggcgag ctgaacttgc aaatcttgtt ctgcccgaat
      961 ttgcttcgct ttacgttcag cttgttgctc tggagttaaa gtacagctac ttaacataag
     1021 tgcgagtgaa aaaatgcagc ctaatttgag tgttttcatt gctgaatcct ctagttgaaa
     1081 ttggtgattt attctaccgc ttctaactca ctttcttcaa gttcatcagc catagcacta
     1141 agggcatcac ggctaagttg tgcgagggct ttataaaaac cattgtcgaa agcttctatt
     1201 tttcctaaga atttgcctgc acaaggtagt aaaaattgtt cgaataatat tttttgagct
     1261 aaggcagaat cgagattatc ttcgatccaa gatgccgtga gaaatagtaa cccaatgtga
     1321 tcggcatttt ctaattcagg cattgcccgc tcttgtctaa aagctacaaa attattaact
     1381 gataatccat aggctgaaat tgttggagaa atagccccat tttcagcaaa aagcgcttga
     1441 tagcttgctg ctaattgcgt agggttagca ttcttttgta agagagtcag cgcattttca
     1501 ctttggctat ccgtatttaa tgcccagact tggcgtaatc ctccttgggc aagccagtta
     1561 aatgtgccag ctaaaatagg gtcggtaggg tgacgataaa ataagttgcc aaacaaacgg
     1621 caaagcaggg aaaaatcgtt aatggtagtt tcgttcataa tttgaaatag atagaaaaaa
     1681 gaattgttag tatagcagaa cacaagagag gactgaatga aatatatagg cgcacacgtt
     1741 agtgcttcag gtggggtgga aaatgcagtt ttgcgttccg tagaaattgg tgctaatgca
     1801 tttgcgttat ttactaagaa tcaacgtcaa tggcaggcac cggcattaaa agccgaaact
     1861 atcgaaaaat ttaagcgttt ttgtaaagtg catcattttt cagcagagca aatcttacca
     1921 cacgatagct atttgattaa tttaggaaat ccggaagcgg aggcattgga gaaatctcgt
     1981 gtagcattta ttgatgagat gtctcgagca aatcaattag gtttaaaatt gttgaatttt
     2041 cacccggggg cgcatttaaa gcaaatttcc gagcaagatt gtttggctag aattgcagaa
     2101 tcgatcaata ttgcagttga aaaagtaccg aatgttattg ccgtgattga aaatacggca
     2161 ggacagggtt ctaatttagg ttggcatttc gatcatttgg caactattat tgagcaagtt
     2221 gaggataaaa gccgagtggg ggtgtgttta gatacttgtc acttattttc tgccggctat
     2281 gatattagtt cttatgaaaa gagcgaggca gtatttaatg attttaacca aacggttggt
     2341 tttcattatt taaggggaat gcacttaaat ggttcaaaaa caccattagg cagcagagtg
     2401 gatcgtcatc ataccttaag agaaggcaca ataggaaccg aagtgtttgc atttattatg
     2461 caaaatatgc attttaacca tattcctatg attttagaga caatttcgcc tgaaatttgg
     2521 gcagatgaaa taaaatttct acgcacattg gaactaaaat aggttatact ggtctgagta
     2581 gtgggtctag cagagtctag accttatttt gttatctttt ttaatctttt agaagattta
     2641 gttaaggaat ttaccgctgt aattgttctg gcgagacgag acataaatta tgaaaaaaaa
     2701 tctttttgca acaaccttca agcccgtatt tattgcttct attttgtctg tttttggagg
     2761 gtattcttcc attgctgtag caagtttaag ttctaaaccg gccacaccaa atcaaatcat
     2821 tgcaagtaaa gcagaaagat tagctaagtc ttcttcagta tttaccaatc gagcaaatct
     2881 tccaacttct tccgctcata cagcgaatta tagtggtcgt attaatagtg tttatcataa
     2941 ttgggtaggg acacgctatc gtttaggcgg cacaacaaaa gcaggaattg attgttccgc
     3001 ctttgtgcgt gaagtaatgg gcaaagcatt tagtaaagat atgcctcgtt caactgcaga
     3061 acaacgccat gtcgggcatt ccatttcaaa atctgattta cgcccaggtg atttagtctt
     3121 tttccgtaag aataaccatg taggggttta tattggtaat ggtaaatttg tacacgctag
     3181 ttcaagtaga ggggtaacca ccagctcatt gtcagaaagc tactggtcaa gaaactacac
     3241 acaatctcgc cgtgtaattt aataaattac aattccaaga tgaaaagaac actcactttt
     3301 gcgagtgttc ttttttaatt aaaaaagttt atgctacgcg ttgcttagct tgtgtaaatg
     3361 ctccacgagt acgcatttga tgaaccacat tagtaattac acctgctatt tttacttcag
     3421 cccaatcagt aattctaaga tttgattcgc tcacatctaa tgaaaataaa attttttcag
     3481 caataccttc cccattctga atttcattaa agaattgata gaatttatat tccccatctt
     3541 tttcaagcac caatagatca ttaactaaat attggttggt ctgttctaca atcaataaat
     3601 cgtctagttc aattccccac gcgattaaat ttgggttttt cacgtggata aaaaaagtgt
     3661 ctcttgggcg tttgatacaa agtgagttaa gatcgagctt tacagagcgg ctgctattga
     3721 aatcccgata aagcggaatc ggctgataag ctgccgtttg atctgaaatt tgtctaattg
     3781 aagccatagt aaaatcctct atctgttttt ttatacaggg tattttactg gctaaaatac
     3841 cagtgtcaac cattattttg gaattttttg taaatttaac cagtgttttg accgcttgtt
     3901 atcctttaaa aagcaataaa aaagcaccca tagggtgctt cttatgctta aaagctgtta
     3961 ttataggctt tcagtgaatg tacgagtaat aacatcgcgt tgttgctcag gggttaatga
     4021 gttgaaacga actgcataac ctgaaacacg gatcgttaat tgaggatatt tctcagggtt
     4081 ttgaatcgca tcttctaatg tttcacggcg taaaacgttt acgtttaagt gctgaccacc
     4141 ttcaacttta atcgttggtg caacattaac gggtacttca cggtatgcga tatcacctaa
     4201 ttcgctgata gcaacaactt gatcttcttc aaatcctgct tttgcagcta aacaacgagc
     4261 ttcattttta gcttcgtcta ataaccagaa agagtttaat aagttgctgt ttgatgattc
     4321 tgtgatttgt acacctttaa tcattttgat atctcctcta agattgaaat tataaaaaaa
     4381 ttttaaaaat tcaaatttat ttgaagttat tgtacccctt tttagataaa aagcgataaa
     4441 aatttaacca tttttagaca tgattgagtt ggttgggtaa aaaatataca cttggtggtt
     4501 ttatgaaaac ttggacagaa gctttaggcg aagaaaaaca aaaagcgtat tttcaacaaa
     4561 ttttacaata tgtacataac gaacgattag ccggcaaagt catctatcct ccgcaaaacg
     4621 aggtgtttag tgcttttgcc ttaaccgaat ttagtgacgt gaaagtagta attttagggc
     4681 aagatcctta tcacggccca aatcaagccc acggattagc cttttcagtt aaaccgccgg
     4741 ttgccccgcc accttctctg gtcaatatgt ataaagaatt atctgaagat attgaaggat
     4801 ttcaaattcc accgcatggc tatttaattg actgggcaaa acaaggggta tttatgttaa
     4861 acaccagctt aacggttgag caaggtagag ccggttcgca ttcacaaatt ggttgggaaa
     4921 tttttaccga taaagtgatc gcacaattaa atcaacaccg tgataattta gtctttctac
     4981 tttggggaag ccacgctcag agaaaggggc aatttatcga tcgtaatcgc cattgtgtgt
     5041 tagcttctcc gcacccatca ccactttccg catatcgtgg attttttggt tgtaaacatt
     5101 tttctaaagc caataactac ttgatttctc aaggaatatc cccaattaat tggcaattat
     5161 cagtagacgt tagttattct tcaacacatt agtttaagga gcaaacgatg aattttgatt
     5221 taccgattgt tagtattgca tttgttgtgc ttgttttagt tgcattaagt tcgactatta
     5281 aaattgtgcc acagggctat cattggacag tagaacgttt tggtcgttat actaaaacgc
     5341 taagcccggg gttgaacatt gtcgtacctt ttattgatcg tattgggcga aaaatgaata
     5401 tgatggagca agttttagat attccatcgc aagaggttat ttcacgtgat aatgcaagtg
     5461 ttgcgattga tgctgtatgt tttgttcaaa cagttgatgc tcgccgagct gcctatgaag
     5521 tgaatcattt agagcaagca attgttaatt taaccatgac aaatatgcgt accgtgctcg
     5581 gctctatgga tctagatgat atgctctctc agcgtgattt aattaacggt agattacttt
     5641 ccattgtcga tgaggcgact aatatttggg gagtaaaagt cacccgtatt gagattcggg
     5701 atgttcgtcc accgaaagag ctggttgcgg caatgaacgc tcaaatgaaa gctgagcgta
     5761 ataaacgtgc tgatattctt gaagcagaag ggattcgaca agctgaaatt ctgcgggctg
     5821 aaggggaaaa acaatctcgt attttaaaag cagaaggtga aagacaagaa gcattcttac
     5881 aggcagaagc tcgtgaacga gccgcagaag cggaagctaa agccactcaa atggtttctg
     5941 aagcaattgc taaaggcgat actacagcga ttaactattt tattgcacaa aaatacaccg
     6001 aagctttgaa agaaataggt tcttcagata acagtaaagt tgtacttatg ccgttagaag
     6061 ctggaaattt gattggttct gttgctggta ttgctgaaat tttaaaaagc aataaaacaa
     6121 gctcatagcc taaaaccgga ttaattgagt atatggaggg agtatggact ggttttttaa
     6181 ttggattggc tggagcatat taggctttgt actacttgca ttagaattag tattaccggg
     6241 tgtctttatt atgtggtggg gatttgcctc gcttattctt gctttcatcg ttgcactttt
     6301 tacattaggg caagcgttac aaattgcgtt atttgcgata attgcgacta tttttagtct
     6361 tatttggtgg aaatatcagc atggaaaaga caagaaagag gatcagagca gttcccttaa
     6421 ctctcgagag catgctatgc taggagctca aggctttgtg gttgaaatct tacctaatgg
     6481 catcgctcga ggaaaatttg gtgatacgac ttggcgtgtt ataggtaata atttacaagt
     6541 agcagataag attgaagtcc agaaagtgga gggaattacc ttatttgtcc gtaagcaata
     6601 agttttttct tatttagtca tagaacacta aacaaaaacc ttgaacagtt gttcaaggtt
     6661 ttttctctca agcggttgaa aatagagtga aatttgcaaa ttttcaccct aaattaaccg
     6721 cttgtgtccc accataataa gttagaaact aaagccaaca tttactttcc aaccaaatag
     6781 gtttgcagat ttcactcgac tgtaatctaa attcatcgca gtaaaccaat ttttataatg
     6841 cagtttcacc ccgacttcgc cgtttaattg gcgattacgt ttcgtcgttg cttcactatg
     6901 cagtgttttt cctgcaatgt tactggtaat tttggttatt tttccattta atctatgcaa
     6961 gtaatttagc ccgatataag gggaaataga gccttgctca agttggaaag ttcgctgtaa
     7021 tttcacgctt ggaacaatat tcacatcacg ataacgcatt gcagccgttt gagtcgcaag
     7081 ttcgccactt tcatttaaac cattcatttt ggtttgaatc cattgtacgc caaggcttgg
     7141 ttcaattatc cattggtttg gcataaattg ataaccgatc cgcatttcac caccgagcgt
     7201 tgaacctctg gtttctgcag tacctagttg atggctagca aatcgacggc tttgttgata
     7261 tttctggcga gcaaattgca atgtagcttc actaaaccaa gcgttatcta aatgataacg
     7321 taagccaata tttaatgctg tttgttttaa ggtagcttgt gcaaatggtt tttctaagcg
     7381 gtttttttgc tgtgaaagtg ttgcactaag taccgtttta tctgccagtt tgtggtttac
     7441 tcccaattgt gattggtggt gtttaacatc ggtatgcttc gatgaggctg attgtttttg
     7501 attcaaatgt tcagcccaaa cagtcgtttg cagtggtgta tggtatgccg taaacaaatg
     7561 ttgctgtgct tgggttaagc ctttggcatt ttggctacgt agcaattcaa ggctatctgc
     7621 ataaatattg ttagatacag catagtgcag attatctaat ttacgttgtt cgagtgcaga
     7681 ttgcagccaa ccgttatacc aactacggcg ataggcaata ggtgtagttc ttaacgcatt
     7741 taataatgct aaaccggctt gcccgtttgt atcggctttc tcatcagtta gtggcttatt
     7801 cacttttgcg ataatttcgc tctctttgaa atagagatct tggattaacc ctcgtttggc
     7861 taaattctcg ttttcgacta attgtgtaaa tccgccttga taagttgctt tatctttcaa
     7921 ctgtactacg gtcgctgaaa catcacctgc ttcggttttt tctgttaatt tatcatcaat
     7981 ttttaacgag ccggctaaat gtatattggc ttgagcattg gcaattagcc cagatttgcc
     8041 caacacttct aattggcttt gtccagacga agaataattt tgctcaactt cggttccatt
     8101 gacagctaaa tttgcatggt tactgattgt tttcacctta gttttaccgt ttaaggccaa
     8161 tctaccttct tcaactgcta ccgtatctaa atgattttcg ccgtctaaat gcagtgattt
     8221 gtcccctttt tttgtaattt taaattggct ggcgagtgga ttcgaccaat ggtcatcacg
     8281 agttacggta atcgtttcat catttaggaa ttgagttggg ccattaacag cttttttcag
     8341 gttaatgaga ccccagccat aaacattatc gacccctttt tcacctaaat cagtggcggt
     8401 agtcaataaa gtatcgcgaa tttgagtcgc agtcagataa tcaaagcgtt cttttaatac
     8461 tgctaatgaa gcagttatag caggtgctga aaatgacgta ccttgctctt ttgttaagcc
     8521 atattgtggt tttttatgtt catcagcaac gccaattaaa acatgtaaat cgcccggtgc
     8581 tgctacacac cagtttttac ttgctccgca gtggttagag tatgaagcaa ttttttgttc
     8641 tttatctacc gcaacggcag aaattaaatt tttctcaagc tcgggaaagt aacgtggtag
     8701 aagagccatt acgcctggct gtttcttgct ctcattacca gcagcaaaga ctaataaggt
     8761 gttgctattt ttggctctca taatagcttg aagtagcgga ttgtgcttat tttgcttgat
     8821 agattgataa aatttatcta caacattgat gtcatcgcta atagaaaaag gattccaact
     8881 attattgatt gcaaaaacat tattatctag taatttattc agcccggatg cccattcagc
     8941 acgttcaatt gccatttctt tttctttgct tgctaataag tcattctttg ccatttgatc
     9001 cagacttggc gttttttcat cctcttcgtc ttttgcattt tctaattctt tcttagcatc
     9061 ttgaataact ttttctaaag ttcgtttata tgacttcgtt gcaacataaa gctcagctcc
     9121 ctttgcaata ccaccactat agccatcacc aagagtttta tctgcttgag cggcaataat
     9181 accagctaca cctgcaccgt gtgtttcaag agaaggaata agatcaattc catcttcttt
     9241 ttctacgggg tttacctcga attgagggta acttgcatcg tagattctta cttcgccttc
     9301 gggtgttgtt aattgatgaa taagaggatg aagatgtgaa ctatggcgag ggtgtttaac
     9361 catgaagccc tcatccatta cgcctagttt aacacccttg ccactatatt ttgagctgag
     9421 gctctcagat aattgaatga taggttgtgg attctctatt gattctgtag cgtatgcaat
     9481 agataaaaaa ctagaaatgc taattgcaat aagtgttttg ttaaatgaat gctttatttt
     9541 atacataatt ttacctccaa aaaataagct tattttacgt agttaatttt t
//
LOCUS       DANLXF010000086         9571 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.086, whole genome shotgun sequence.
ACCESSION   DANLXF010000086 DANLXF010000000
VERSION     DANLXF010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9571
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.086"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(210..1343)
                     /locus_tag="PXD66_001346"
     CDS             complement(210..1343)
                     /locus_tag="PXD66_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL6319643.1"
                     /translation="MKTIKIGLVGTGYIGRCHAIAYAQAPTLFPLKGKIQLEYLAEIN
                     QALADQKAREFGFQRATDDWRKLVTDPNVDVVDICTPNFLHKEIALEAIKHGKHVYSE
                     KPLALTAQDAKEMVIAAQQAGVKTLVGFNYIKNPTTQLARQIIQNGEIGEVVHFYGTH
                     NEDYLANPNTPIDWHCYKAKAGLGTLGDLAAHIVSIAHYLVGSDIERVIGDMQTVIEE
                     RPSPHNPAEKIKVENEDQASSLIRFANGVMGTLESSRISCGRKMGLTYVVTGTKGTLS
                     YTQERMAELKLYLHDDDKSRQGFKTILVGPEHPDYAAFCVSAGHGIGFNDQKAVEIRD
                     LINGIAADDPMYPDFEEGYKISRVLEAIAKSAEEKRWVEVSEI"
     gene            complement(1474..2502)
                     /locus_tag="PXD66_001347"
     CDS             complement(1474..2502)
                     /locus_tag="PXD66_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL6319644.1"
                     /translation="MTTQFLKNMGMVDDKGKIDFIAFFERFGVLIFLILLIIFFTSQN
                     GAFLSQRNIYNVLTEVSIFGIMAVGMTFVILTAGIDLSVGSILAVTAMFAAYIIKGDN
                     TVTLEAGAWGGMSWLIGLSICLGLGTFIGWLHGLGVTKLKLPPFIITLGGMTIWRGLT
                     LVLNNGSPIAGFDEGYRWWGRGDILGVPVPVVIFAVVAIIGYFALHKTRWGRYVYSVG
                     GNPEAARLAGVNINRTLVSVYVVIGALAGLAGFILSARLGSAESVAGVSFELRVIASV
                     VIGGTSLMGGYGRVTGTIIGSIIMGVLINGLVLMDVSAYYQQIIMGLIIILAVAFDTY
                     AKSRRGAL"
     gene            complement(2514..4058)
                     /locus_tag="PXD66_001348"
     CDS             complement(2514..4058)
                     /locus_tag="PXD66_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL6319645.1"
                     /translation="MSTPLLEVKDLTKSFSGVMALNGVKLTVGKGEVHALLGENGAGK
                     STLLKALSGAQPQTSGEIIFNGETLSLSDSPFDRQLKGIVTIYQEFNLLPNMTVAENF
                     FLGREPVKGLFVNEKAVNEEAQLVLDNLGLNIKPDTQVSRLSVAQQQMVEIARAMTLN
                     AKLIIMDEPSAALSDKEVETLHQIVRDLKSRGISVIYVTHRLNEVFALCDRFTVFQDG
                     RYSGEGEVKNVNVDQIIKMMVGREVAFTRRPPEETHHTHRPIRLAVKNLHREKPAFDP
                     HGIALHGVSFNIHEGEVLGIAGLVGAGRTEIARCLFGVENYTADEISLDGKPYAATSP
                     LDALEKGVALVPEDRKKEGLVLGLPIKTNMTLPILSKLLKGNLFVDNQKEDDLIETYR
                     QALRIKMASSELEARKLSGGNQQKVILARCMALNPKVLIVDEPTRGIDVGAKSEVHQV
                     LFDMAKQGVAVLVISSDLPEIMAISDRIITLSEGRVTGEIHGDDATEEKLMSMMAVGV
                     QRKAAA"
     gene            complement(4109..5047)
                     /locus_tag="PXD66_001349"
     CDS             complement(4109..5047)
                     /locus_tag="PXD66_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDL6319646.1"
                     /translation="MKKRIKFVLSALTLGLAMNSFAKDELVVFSLPNLSSPFEVQLQK
                     VAVETSKKLEIKLQVLDGQSSSTKQASDLENAITRGAKGIIISPNDVNAISGAVEEII
                     KEKIPAATLDRKVESSKPVPHFGANNYTGGQEVAKAVKAKYPNGAKIILLTGQPGSTS
                     NIERTKGIRDELAAGGDKYKIVVDQTGNWLRSEGLRIIESVLPTLKEKPEVIISANDD
                     MALGAIEALRSQGLKAGDILVTGFDSTPEALARVKDGWLYLTADQRPSFAVSTALEQV
                     VGNIRESKEVSGADYPPKIILKDNLQEAERFAEISE"
     gene            complement(5379..6890)
                     /locus_tag="PXD66_001350"
     CDS             complement(5379..6890)
                     /locus_tag="PXD66_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HDL6319647.1"
                     /translation="MSQFVQNFINGAVVESQSQRVTSVFNPATGEETKKVKLSLPNEV
                     DAAIAAADQAFKSWSQQSPLRRARILFKFKELLEANFDELARLISSEHGKIYSDAIGE
                     LTRGLEVVEFATGIPHLQKGEFSANAGRGIDIHSIQQPLGVVAGITPFNFPAMVPMWM
                     FPVALACGNTFVLKPSEKDPSVSIRLAELLKEAGLPDGVFNVVQGDKEAVDILLTDSR
                     IQAVSFVGSTAIAQYIYEKGSANGKRVQALGGAKNHALIMPDADVQGTINALLGAAFG
                     AAGERCMALSVAVVVGDELADKIIEELIPKVKALKIGSGLLPQGVLENDMGPVISKEH
                     KAKIESYIDQGVAQGAKLCVDGRGYKVEGFENGYFVGGTLFDNVTPEMTIWQDEIFGP
                     VLSVVRVKDYAEGIALINSHQYGNGSAIFTADGDSARQFTQDVQAGMVGVNIPIPVPM
                     AFHCFGGWKASIFGPLNVYGTDGVRFYTRMKTITTRWPDSSVRSQAAFNFPTL"
     gene            7109..7933
                     /gene="iolB"
                     /locus_tag="PXD66_001351"
     CDS             7109..7933
                     /gene="iolB"
                     /locus_tag="PXD66_001351"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HDL6319648.1"
                     /translation="MSSPLLSKPNFNQHEGLIQHITPENAQWEYVGFDVYQLSKQQIT
                     LESGENELCLVFVTGFGSVYVDDYAFENIGNRTTPFEKIPPYAVYIPPHKSVLVKTEL
                     NLELAVCKAPSQGNYPVRLISPNHIGVEKRGFGNNQRLVHNILPETEAADSLLVVEVF
                     TDEGNTSSYPSHKHDDKHSTEETYLEETYYHRFDPPQGFAMQRVYTDDRTLDECMAVY
                     DKNVVKVPRGYHPVATIAGYNNYYLNVMAGPVRKWKFTWEQDHLWVNSETYKAKFE"
     gene            complement(8009..9460)
                     /locus_tag="PXD66_001352"
     CDS             complement(8009..9460)
                     /locus_tag="PXD66_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HDL6319649.1"
                     /translation="MPNHKFLLLRFQAYINGKFVSSQNGKTFTVQNPTNGEKIGEVAD
                     LGTEETAQAIEAAAVAQKKWQALLPKERSVILRKWFELIMQNQEELAQILSLEQGKPL
                     AESRGEIAYGASFIEWFAEESKRIYGDVLPQDKAGRRLFVTKQPIGVVAAITPWNFPN
                     AMITRKAAPALAAGCAIVIKPAAETPLSALALAQLADQAGIPKGVFNIITSSDVEGVG
                     RELSTNPTVRKLTFTGSTRVGKILMQQSAGTVKKLALELGGNAPAIVFDDADLDTAVE
                     GVFASKFRNGGQTCVCANRIYVQAGIYEQFLAKLSEKVAQIKLGSPFEEEVTIGALIS
                     ERAVEKVESHINDALSLGAELVLGGERHALGGTFFQPTILKNVTQAMKVAREETFAPL
                     APIFKFETEEEVIQWANDTEFGLAAYLFSQNIGRIWRVSEQLEYGIVGINEGIISNEI
                     APFGGWKESGLGREGSKYGIEEFLEIKYLCMKV"
BASE COUNT         2611 a         2271 c         1979 g         2710 t
ORIGIN      
        1 tagataggca cttgatgaac aagttggaag taagcatcca aataaacaac tatcaatatg
       61 aagcctaaaa accattctgt ttgcataaga acattgatag ggctgttttt tgtgctaaaa
      121 atttctttaa ttgaactcat aatttttcct taaatgtaaa aaaacgtacc tcaaccaatt
      181 tgagatacgt ttctatttta atgtaattat taaatctcac tcacctccac ccaccttttc
      241 tcctccgccg attttgctat cgcttccaat actctggaga ttttatagcc ttcttcaaaa
      301 tcgggataca tcgggtcatc agcggcgatg ccgttgatga ggtcacggat ttccaccgct
      361 ttttggtcgt taaaaccgat gccgtgaccg gcactcacac aaaaagcagc gtagtcggga
      421 tgttccgggc cgactaaaat ggttttgaat ccttggcggg atttatcgtc atcgtgcaaa
      481 tagagtttga gttctgccat tcgttcttgg gtatagctga gcgtgccttt agtacctgta
      541 acgacatagg tcagccccat tttacgcccg caggaaatgc gggagctttc gagcgtgccc
      601 attaccccat tagcaaatcg tatgagacta cttgcctgat cttcgttttc tactttgatt
      661 ttttctgctg gattgtgcgg actagggcgt tcttcaatca ctgtttgcat atcgccgata
      721 acacgctcaa tgtctgaacc aaccaaatag tgtgcgatac tgacaatatg tgcggctaaa
      781 tctcccaacg taccaagccc ggctttggct ttgtagcagt gccaatcaat cggggtatta
      841 gggttggcga gataatcttc gttatgcgtg ccgtagaaat gcaccacctc accaatttcg
      901 ccgttttgaa taatttggcg agccagttgg gtggttggat ttttgatata gttgaaaccg
      961 accaaggttt taactcccgc ttgttgagcg gcaatcacca tttctttggc atcttgggcg
     1021 gtgagtgcaa gcggtttttc tgaatagacg tgtttgccgt gtttaatcgc ttctaaggca
     1081 atttctttgt gcagaaaatt tggtgtacaa atgtccacca catcgacatt cggatcggta
     1141 accaattttc gccaatcgtc agtagcacgc tgaaagccaa attctcgggc tttttgatcg
     1201 gctaatgctt ggttgatttc ggcaaggtat tccagttgga ttttgccttt aagtggaaac
     1261 agggttgggg cttgagcgta ggcaatggcg tggcagcgac cgatatagcc ggtgccaact
     1321 aagccgattt tgatggtttt cataatacta tcccagtagg gtgcgtttgc taacgcacca
     1381 ttagaatttt cagtgcattg gcaaacgcac cctataagaa agcggtctgg ttttgcaaaa
     1441 tttttacaaa atcagaccgc ttgtgttagc tatctataat gctcctcgtc tgctcttcgc
     1501 ataggtgtcg aaggcaacgg caaggatgat gattaagccc ataatgattt gttggtagta
     1561 agcggaaacg tccattagta ctaaaccgtt aatcaataca cccataataa tggagccgat
     1621 gatggtgcct gtgactcgtc cgtatccccc cattagggaa gttccgccga taaccactga
     1681 ggcaattaca cgtagctcaa aagaaacgcc ggcaacggat tcggcactgc ctaaacgggc
     1741 ggagagaatg aaaccggcaa gtcctgccaa tgctccgatc accacataaa cactgactaa
     1801 ggtacggtta atattcacac cggctaaacg ggctgcttcc ggattgccac ctaccgagta
     1861 cacatagcgt ccccagcggg ttttatgcaa tgcgaagtaa ccgataatgg cgactacggc
     1921 aaaaatcact accggcaccg gtacgcctaa aatatcgcca cgtccccacc agcggtaacc
     1981 ttcgtcaaat ccggcaattg gcgagccgtt atttaacaca agtgttaaac cacgccaaat
     2041 tgtcataccg ccaagcgtga taataaacgg tggcagtttg agtttagtga cgcctaagcc
     2101 gtgtaaccaa ccaatgaaag tgcctaaacc taaacagata ctcaaaccta tcagccagct
     2161 cattccgccc caagcaccgg cttctagcgt taccgtatta tcgcctttga taatataggc
     2221 cgcgaacatt gcggtaacag caaggattga gccgacagag agatcgatcc ctgcagttaa
     2281 gatgacgaaa gtcattccta ccgccataat gccgaaaatc gacacttcgg ttaaaacgtt
     2341 atagatgttg cgttgcgata aaaatgcccc gttttgtgaa gtgaagaaaa taatcagtaa
     2401 gatcaagaaa atcagtacgc cgaaacgttc aaagaaggcg ataaaatcga tttttccttt
     2461 gtcatcgacc atacccatat tttttagaaa ttgcgttgtc atacaaacct ctcttatgct
     2521 gccgctttac gctgaacgcc aacagccatc attgacatta atttttcttc ggtggcatca
     2581 tcgccgtgaa tttcgccagt tacccgacct tcggaaagag tgataatgcg gtcggaaatc
     2641 gccataattt ccggtaaatc ggaggaaatc actaataccg caacaccttg ttttgccata
     2701 tcaaacagca cttggtgaac ctcagatttt gccccaacat caatacctcg tgtcggctca
     2761 tcaacaatta gtactttcgg gttgagtgcc atacaacggg ctaaaatcac tttttgctga
     2821 ttaccacccg ataatttgcg ggcttcaagt tcgctacttg ccattttgat gcgtaacgct
     2881 tggcggtagg tttcgattaa atcatcttct ttttgattat cgacaaacaa gttacccttc
     2941 aataatttgc ttaaaatcgg cagggtcata ttggttttaa tcggtaaacc gagaactaag
     3001 ccctcttttt tgcgatcttc cggcacaagg gcaacccctt tttccaaagc atctaacggc
     3061 gaggtggcag cataaggctt accgtctagg ctgatttcat cagccgtata attttctacc
     3121 ccaaacaagc aacgggcaat ttcagttcgc cctgcgccaa ctaagccggc gatccctaat
     3181 acttcaccct cgtgaatgtt aaaagagaca ccgtggagag caatgccgtg tggatcgaat
     3241 gccggttttt cccgatgcag atttttcaca gctaaacgta ttggtcggtg ggtatggtga
     3301 gtctcttctg gtggacggcg agtaaaggca acttcacgtc ctaccatcat tttaataatt
     3361 tgatcgacat tcacattttt gacttcaccc tcaccagaat agcgcccgtc ttggaaaacg
     3421 gtgaaacggt cgcatagggc aaacacttcg tttaagcggt gggtcacata aatcacgcta
     3481 atgccacggc tttttaaatc acgcacgatt tggtgtagtg tttccacttc tttatcactt
     3541 aaggcagcgg acggttcgtc cataataatc aacttagcgt ttaaggtcat tgcacgggca
     3601 atctcgacca tctgctgttg tgccacactt aaacgggaaa cttgggtgtc aggtttgata
     3661 tttaagccga gattatccag cactaattgt gcttcctcat tcaccgcttt ttcattgacg
     3721 aataaacctt tcaccggctc tctgcccaaa aagaaatttt cagcaaccgt catattcggt
     3781 aacaagttaa attcttgata aatcgttacg atgcctttga gttggcgatc aaacggggaa
     3841 tcgctcaaac ttaaggtttc accgttaaag ataatttcgc cacttgtttg tggctgagcg
     3901 cctgaaagtg cttttaataa ggtcgatttt cctgcaccgt tttcacccaa caatgcgtga
     3961 acttcgcctt ttcccacagt cagtttcacg ccattgagtg ccataacacc agagaaactt
     4021 tttgtcagat ctttcacttc aagtaaggga gtagacatac aacctccgta aaaaaacgcc
     4081 ctccggcctc agccggaagg cagataaatt attcgctgat ttctgcaaaa cgttctgctt
     4141 cttgcagatt atctttcagg ataatttttg gcggatagtc cgcaccgctc acttctttgc
     4201 tctcacggat attgccaacc acttgctcta atgcggtgct taccgcaaaa cttgggcgtt
     4261 ggtcagcggt gagatacaac cagccatctt tcacacgtgc aagagcctca ggggttgagt
     4321 caaagccggt tactaaaata tcgccggctt tcaaaccttg gctacgcaat gcttcaatcg
     4381 caccaagtgc catatcgtca ttcgctgaaa taatcacttc tggtttctct tttaaggttg
     4441 gtaacacact ttcaataatg cgtaatccct cagaacggag ccagttaccg gtttgatcaa
     4501 ccacgatttt atatttgtca ccaccggcag ctaattcatc acgaatacct ttggtacgtt
     4561 caatgttaga ggttgaaccc ggctggccgg ttaaaagaat aatttttgcc ccattcgggt
     4621 atttggcttt cactgctttt gcgacttctt gaccgcctgt gtagttattt gccccaaagt
     4681 gtggaaccgg cttactgctt tcaacttttc ggtctaatgt cgcagcagga attttttctt
     4741 tgataatttc ttccactgca cctgaaatcg cattcacatc attcggggaa atgataatgc
     4801 ctttagcacc acgggtaatt gcattttcta agtcagaagc ctgcttagta gaagaacttt
     4861 gcccgtctaa tacttgcaat ttgatctcca actttttact ggtttctacc gctacttttt
     4921 gtagttgaac ttcaaacggg ctggataggt tcggtaagct aaataccaca agctcatctt
     4981 tcgcaaagct gttcattgct aagcctaacg ttaaagcact caatacaaat ttaatacgct
     5041 ttttcattac aaaatcctct ttttaagatg ggaattgacg gcgaatattg ccaatgttac
     5101 ttcaaactgc gaaggaaact taagtcttat ttcaataata aacctcaatg aaataaattt
     5161 ttcaatttag aatatatcat tcaaaatttt ccaaaccaaa tgctttgttt caaatttgtt
     5221 acgtagatca catttttagg aagagataaa tgcaagcggt caaaacaggt cgcttttttg
     5281 caaaaatttg aataaaaatg accgcttgtg agatgaaaac caataaaaaa gcggtgattt
     5341 ctcaccgcta aacactcaaa aaagaaatgt actcgttatt ataaggttgg gaaattaaat
     5401 gccgcttggc tacgcaccga gctgtccggc caacgggttg tgatggtttt catacgggtg
     5461 tagaaacgca cgccgtctgt gccgtacaca ttgagaggcc cgaaaataga ggctttccaa
     5521 ccgccaaagc agtggaacgc cattggcaca ggaattggaa tattcacgcc caccataccg
     5581 gcttgtacat cttgggtgaa ttgacgtgct gaatcgccat ctgcggtgaa aattgcacta
     5641 ccattgccgt attggtgact gttaatgagt gcgatccctt cggcataatc tttcacacgc
     5701 acaacagaga gaaccggtcc gaagatttca tcttgccaga tggtcatttc aggtgtcacg
     5761 ttatcaaaca gcgtacctcc gacaaagtaa ccgttttcaa acccttcgac tttatagcct
     5821 cgaccatcga cacagagttt cgccccctgt gccacccctt gatcgatata gctctcaatt
     5881 ttggctttat gctctttgga aatcaccggc cccatatcat tttcaagcac gccttgtggt
     5941 agtaagcctg aaccgatttt caatgctttg accttaggaa ttagctcttc aatgatttta
     6001 tcggctaatt catcaccaac aactaccgct accgatagtg ccatacaacg ttcaccggct
     6061 gcaccaaatg ctgcgcccag taaggcatta attgtgcctt gtacatcagc atccggcata
     6121 atcaaagcgt ggtttttcgc accgcccaag gcttgaaccc gtttgccgtt tgctgagcct
     6181 ttctcataaa tatattgtgc aattgcagtt gagccgacaa aactcaccgc ctgaatgcga
     6241 gagtcggtta aaagaatatc taccgcttct ttatcacctt gcaccacatt gaatacaccg
     6301 tccggcaagc cggcttcttt taataattct gccaagcgaa tagacacact tgggtctttt
     6361 tcagacggtt ttaaaacgaa agtgttaccg caagcaagtg ccactgggaa catccacatc
     6421 ggtaccattg ccgggaaatt gaacggggta atgcctgcca ctacgcccaa cggttgctgg
     6481 attgagtgaa tatcaatgcc acgccctgca ttggcggaga actcaccttt ttgtaagtgt
     6541 ggaataccag tggcaaattc caccacttct aaaccacggg ttaattcgcc aatcgcatcg
     6601 gaatagattt tgccgtgttc tgagctgatt aaacgagcca actcatcaaa gtttgcttcc
     6661 aataactctt tgaatttaaa taaaattctg gcacggcgta gtggtgattg ttgtgaccag
     6721 cttttgaaag cctgatcggc cgctgcaatt gccgcatcga cttcgtttgg caggcttaat
     6781 ttgacttttt tggtctcttc accggttgcg ggattaaata ccgaggtaac acgctggctt
     6841 tggctttcaa cgactgcacc gttaataaaa ttttggacaa attggctcat tggaggctcc
     6901 ttatcaactg attgagagga aaaacggata aaatacagcc gataaactac cgctaaaaat
     6961 ggaaaatatc aatacataat actaaaaaat gaaaatttta tttcattttt gtgatctaag
     7021 tagcaagttt acagattgcc ggtattaaaa tgagacaaaa tttcgtatag tttgaaagcg
     7081 tattccattt cgttaagcga ggtttcctat gtctagtcca ttattgtcta aacccaattt
     7141 caaccaacat gaagggttaa ttcagcacat tacccctgaa aatgcacagt gggaatatgt
     7201 tggatttgat gtttatcagc tcagtaagca acaaattacg ctggaaagtg gtgaaaatga
     7261 actttgtcta gtctttgtga ccggttttgg ctctgtctat gttgatgatt atgcttttga
     7321 aaatatcggc aaccgtacca cgccatttga aaaaattccg ccttatgcgg tctatatccc
     7381 gccacacaaa agcgtgttgg ttaaaaccga actcaattta gaattggcag tgtgcaaagc
     7441 accaagtcag ggtaattatc cggtgcgttt gattagcccg aaccatatcg gtgtggaaaa
     7501 acggggcttt ggcaacaatc aacgcttggt gcataatatt ctgccggaaa ccgaagcagc
     7561 cgacagtttg ctggtggtgg aagtctttac cgatgaaggc aataccagtt cctacccaag
     7621 ccacaagcac gatgacaaac actctaccga agaaacctat ttggaagaga cctactacca
     7681 ccgcttcgat ccaccacaag gttttgcaat gcaacgggtt tataccgatg atcgcacact
     7741 tgatgaatgt atggcggtgt atgataaaaa tgtggttaaa gtgcctcgtg gctaccaccc
     7801 tgttgctacc attgccggct ataacaacta ctacctaaat gttatggcag ggccggtacg
     7861 caaatggaaa tttacttggg aacaagatca tctttgggtg aacagtgaaa cctataaagc
     7921 caaatttgag taatgcaagc ggtctttttc caccaatttt ttacaaaata agggattaaa
     7981 aatgggacgc ttttggcgtc ccattcggtt atactttcat acaaagatat tttatttcta
     8041 aaaactcttc aataccgtac ttcgaacctt cccgcccaag tccagactct ttccagccgc
     8101 cgaacggagc gatttcgtta ctgatgatcc cctcattaat cccgacaatg ccatattcca
     8161 actgctccga tactcgccaa attcgcccga tattttggct gaataagtaa gccgccaaac
     8221 caaactcagt atcattcgcc cactggataa cctcttcttc ggtttcaaat ttgaaaatcg
     8281 gggcaagggg agcaaaggtt tcctcacgag ccactttcat cgcttgagtg acatttttca
     8341 gaatggttgg ctggaagaat gtgccaccca gtgcgtggcg ttcaccgcct aacaccagtt
     8401 ctgccccaag tgaaagggca tcgttaatgt ggctttcgac tttttccacc gctcgttcac
     8461 taattagtgc acctatcgtg acctcttcct caaatggcga gccaagcttg atttgagcca
     8521 ctttctcact caattttgca agaaattgct cataaatgcc cgcttgcaca taaattcggt
     8581 tagcacagac gcaggtttgc ccaccgttac ggaatttaga ggcaaacacg ccctcaaccg
     8641 ctgtatctag atccgcatca tcaaacacaa tcgccggtgc attaccgcct agctccaatg
     8701 ccaatttttt taccgtccct gccgattgtt gcattaaaat tttgccgaca cgggtggagc
     8761 cggtaaaggt gagtttgcgt acggtcggat tagtgctcaa ctcccgccct acaccttcga
     8821 catcgctact ggtaataatg ttaaataccc ctttgggaat acctgcttga tccgctaatt
     8881 gggctaatgc taaggcagaa agcggggttt ctgccgccgg tttaatcaca atcgcacaac
     8941 ctgccgctaa cgccggtgcc gctttacggg taatcattgc atttgggaaa ttccacggtg
     9001 taattgctgc caccacgcca atgggttgtt tagtcacaaa caagcggcga ccggctttat
     9061 cttgtggcaa gacatcgcca taaatccgct tgctttcttc ggcaaaccac tcgataaaac
     9121 tggctccata ggcaatctcg cctcgacttt ctgccaatgg tttaccctgt tctagactta
     9181 aaatctgggc gagttcttct tggttttgca taatcagctc aaaccattta cgcaaaatca
     9241 cggaacgctc tttgggcaat aatgcctgcc attttttctg ggcaactgcg gctgcttcaa
     9301 tcgcttgagc ggtttcttcc gtgcctaaat cagccacctc accgattttc tcaccattag
     9361 tcggattctg taccgtaaag gttttcccat tttggcttga aacaaattta ccgttaatat
     9421 aggcttggaa acggagtagc aaaaatttgt gatttggcat aatcactcct tgaatggtta
     9481 aaaagagaac aatattgact atgatccaat ttcagtaaag agaaaagagc tgaaaaataa
     9541 ctttttactc aataataaag caccttagac a
//
LOCUS       DANLXF010000087         9484 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.087, whole genome shotgun sequence.
ACCESSION   DANLXF010000087 DANLXF010000000
VERSION     DANLXF010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9484
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.087"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..323
                     /locus_tag="PXD66_001353"
     CDS             <1..323
                     /locus_tag="PXD66_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319650.1"
                     /translation="EYSKFNLENVLIDFPNKTNSVAYQNLVIHNAKTRAGKLKTQYAN
                     AHDLGNLVLVEDIQKIFSGLLEIIYQVRCHLVHGSLSPTPENHEVVKYCYLILWDCLK
                     GFCD"
     gene            complement(591..1328)
                     /locus_tag="PXD66_001354"
     CDS             complement(591..1328)
                     /locus_tag="PXD66_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL6319651.1"
                     /translation="MIKIENLTLAHGIQHLSCYLPKGKLIGIMGANGAGKSSLLKAIA
                     GILRPTSGEIWLENCKLHALSAVERSRQFAYLAQQLNVAWQLSVYDVIALGLPAPLPP
                     EQERQKVRSVAEQLAISHLLDNTYQQLSGGEQTRVQLARCLMKQAPILLADEPIAALD
                     PYYQIDIMQQLKALTPTQTCVVVIHHLPLAYRFCDEIILLKNGNLLVSGNTQAVLNAE
                     NIATAFGVKAEMDWEKREMWGIEKLEV"
     gene            complement(1325..2293)
                     /locus_tag="PXD66_001355"
     CDS             complement(1325..2293)
                     /locus_tag="PXD66_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL6319652.1"
                     /translation="MKKLNIVLGSLLLLIVSNAAFYQLSDFALLKNAEGVLSDLRWLV
                     LGEIRLPRIGLAVLTGASLALAGNAMQGVFKNPLASPGLLGSSSGATAVSVFLLYYFS
                     APMGWLLFGGVAGALGAFLLVWLISFRHGTTMMILSGVAVNVLLGAAVTLLLSNAESP
                     WALAELYRWLQGSLVWATAEAVCWALPLILLGIVCLYRERRYLDLLTFGEETAATMGI
                     NLQRSFFTVSIGVALLVGATIPQTGTIGFIGLIAPHLARIWLKKPPSQLYLTSALIGA
                     LLLLIADLAVQYVPFFARIYIGTLTAILGVPFLIWILFTQQWRLAQ"
     gene            2474..2809
                     /locus_tag="PXD66_001356"
     CDS             2474..2809
                     /locus_tag="PXD66_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL6319653.1"
                     /translation="MYHIERTEIFDQWLENLKDPLAVVTIARRIERAESGNFGDTKYI
                     AEGISEMRLNVSKGYRLYYAQKGSIIYILLNGGHKGTQQADIDKAKMLWAEIKQHMAE
                     QEKKNERII"
     gene            2793..3116
                     /locus_tag="PXD66_001357"
     CDS             2793..3116
                     /locus_tag="PXD66_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative addiction module antidote protein"
                     /protein_id="HDL6319654.1"
                     /translation="MNELYDAPIPQATREKFGIQPFDAAEYLANDELIALYLAETLKD
                     GTDEEFIQALNTVARAKGMNELAKKAGIGRESLYQSLSSSKPRFETIRKIISALNLEL
                     SVVKA"
     gene            complement(3106..4992)
                     /locus_tag="PXD66_001358"
     CDS             complement(3106..4992)
                     /locus_tag="PXD66_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HDL6319655.1"
                     /translation="MTQLDSVTVYSAYATPINQDKTASSVTVLTEKDFAARNATYVSD
                     VLKTVPGVAIGQQGGRGTLTSLFLRGAESRHTAVVIDGVKVNPINIGNFDFGGLPISN
                     IERIEVLRGEQSALWGSSAMGGVVYITTKSGLYKEKPFNAEVDLGLGSNNTRDASATL
                     SGFHNGFYYALHGDSHRTKGISALSKNHFSYTTETGSEVKTGGASERDGFHRDNGSLR
                     LGYDLGNKGVEVLAAQSSQTVHIDGYNSDVSGEYSRTRNQTFKLGGYWGNEQELLKHQ
                     ANISQFNSKATHFGSNARYSNEKQLNANYQLDVNFDREGEVTQAVSLLTDYAKTRYTS
                     DKYLREKTLSEKSAALEYRLFTEQDHSFSISGRYTDNSQFKNSITGRISGAYRLSPNL
                     CSDRLLLELAEPQQIRAMSPYSQKPLMMLDKLNTDKPTVEPELTALLPYADSTILLNE
                     TFYPQLTARLKQLGFKLVALNDSPQTPEQLFTLILQLGELTQNQAKAEKLVERLRLQK
                     IPLKQPLAETLILSETGMIEPHFPQYQTLLDLLGLSPLKSDLTPQNFSLEKLLLAQPK
                     QLLFLTDNQSYNNQAELLKHPALQKIWQKMSQNPPLVLPMKYTYCFDHGVWQGIQLMH
                     KLTP"
     gene            5022..5822
                     /gene="metF"
                     /locus_tag="PXD66_001359"
     CDS             5022..5822
                     /gene="metF"
                     /locus_tag="PXD66_001359"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HDL6319656.1"
                     /translation="MQQKWRMLWDSIDRLSPLKPKFVSVTYGANSGERDRTHSIVKSI
                     QEKTGIISAPHLTGIDATPEELKVIAKDYWDSGIRRIVALRGDIPKGYDKALFYASDL
                     VALLKSVADFDISVAAYPEVHPEAKSAQADLLNLKRKIDAGANHVITQFFFDVESYLR
                     FRDRCVSIGIDCEIVPGILPVTNFKQLQKMAAVTNVKIPTWMGKMYEGLDDDQTTRNL
                     VAASIAMDMVKVLSREGVNDFHFYTLNRSELTYAMCHTLGVRPDLTTA"
     gene            complement(5871..7031)
                     /locus_tag="PXD66_001360"
     CDS             complement(5871..7031)
                     /locus_tag="PXD66_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319657.1"
                     /translation="MKKSILLILSLFSASLFADVQLSPLPLPQIGKVSQFDLSEQGEL
                     VVINQQKQLWRVTQQAQKLADGVSTEIEPKAQYQRIALADQNGHFMLWTADKIYTSNI
                     PLAPKATMTALNFATIAVTKQNGQFKLARIETQGNQATITATAETEVLPDAHPLQIDF
                     DAETPNQGHIAVLAKPDDFTYQHGVLGDDVEAGEIQFLERHTLNPLAEKLTQQGRVFE
                     ANRWATFTENGKTKIVSVISGDGNGASTVVIGVKDGKLSIEAESEPLSTNRWQSPFIF
                     NRKLYAIQMPHLKGNLVEYSLKGNQLAERFITSGLSNHRYGDHETNLVATTETFALIP
                     QMGYQQVVVLDKTGKITELPTALPAGIKQTKADSNKAYLLLKNGEIWVAEEK"
     gene            complement(7091..8107)
                     /gene="galE"
                     /locus_tag="PXD66_001361"
     CDS             complement(7091..8107)
                     /gene="galE"
                     /locus_tag="PXD66_001361"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDL6319658.1"
                     /translation="MAILVTGGAGYIGSHTLVELLNENREIVVLDNLSNSSEVSLERV
                     KQITGKSVKFYQGDILDRDILRKIFAENQIESVIHFAGLKAVGESVREPLRYYQNNVT
                     GSIVLVEEMLKANVNTIVFSSSATVYGDPQIIPIVESCPVGGTTNPYGTSKYMVERIL
                     EDTVKAFPQLSAVVLRYFNPVGAHESGLIGEDPNGIPNNLMPFISQVAVGKLPQLSVF
                     GGDYNTHDGTGVRDYIHVVDLALGHLKALDKHQNDAGFHVYNLGTGTGYSVLDMVKAF
                     EAANGITIPYKVVDRRPGDIAVCYSAPQKALEQLGWETERGLEQMMKDTWNWQKNNPN
                     GYKG"
     gene            complement(8195..8356)
                     /locus_tag="PXD66_001362"
     CDS             complement(8195..8356)
                     /locus_tag="PXD66_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HDL6319659.1"
                     /translation="MNETLLNNLVCPVSNEKLEWDKENNRLINPKLNIAYPIKNGIPE
                     LLPEAGVAL"
     gene            complement(8334..9335)
                     /gene="lpxK"
                     /locus_tag="PXD66_001363"
     CDS             complement(8334..9335)
                     /gene="lpxK"
                     /locus_tag="PXD66_001363"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HDL6319660.1"
                     /translation="MKIWQTKSLITWLLSPLSLLFWLISQARKRLYSLNILQSYRSPL
                     PMLVVGNISVGGNGKTPVVVWLVEQLQQRGVKVGVISRGYGGKAKTFPQLVTRESSAE
                     MVGDEPVLIVQRTNVPLAISPNRQQSIELLLNQFEPDIIITDDGLQHYALQRDMEWVV
                     VDGVRRFGNGFVLPAGGLRELPSRLNSVQAVICNGGTPQANEHLMTLEPSDAINLVTN
                     QRKPLSEFTQPCVAIAGIGHPPRFFTMLEKLGIPLAKTQGFADHQAFDKAQLAQFADS
                     QTPLLMTEKDAVKCHDFAQPNWWYVPVSATFSEKSTACLLAPILQKLGKEDERNTTQ"
     gene            complement(9337..>9484)
                     /locus_tag="PXD66_001364"
     CDS             complement(9337..>9484)
                     /locus_tag="PXD66_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL6319661.1"
                     /translation="GNGVEEIQRLQLIISHKDELLAHKEEIIENQKRELALLRDLVET
                     LKNK"
BASE COUNT         2595 a         2066 c         2106 g         2717 t
ORIGIN      
        1 gtgaatattc taaatttaac ttggaaaatg tgcttattga ttttcctaac aagacaaatt
       61 ctgtagcata tcagaatcta gttattcaca acgcaaaaac gcgtgcagga aaattaaaaa
      121 cacaatatgc aaatgctcac gatttaggca atttggtttt agtagaggat atccaaaaaa
      181 tattttccgg gttattagaa ataatttatc aagttcgttg ccatttggta catggtagct
      241 taagtccaac acctgaaaac cacgaagttg ttaaatattg ctatttgatt ttgtgggatt
      301 gtttaaaagg attttgtgat tagttattgt caataaagct acaagcggtc aattctccca
      361 aaactttgca aatttccccg caaaactgac cgcttgttct cctctatcca ttcacctcaa
      421 ccaacttttc tctctacccc tactcaaaat cggttaaaca atctgttgag gtaaatgttg
      481 tgtcggaatg ggatttaatc ttaatgaaac tgaaaaaatc ttctgccaag caaatttccc
      541 taaaattgga agttcatttc caatttctca aaaaatcacc caaaccggaa ctacacttcc
      601 aatttttcaa tcccccacat ttcccttttc tcccaatcca tttcagcttt caccccaaaa
      661 gccgttgcaa tattttcggc atttaagacc gcttgtgtgt tgccgctgac gagtaaattg
      721 ccgtttttaa gcagaatgat ttcatcacaa aaacggtagg caaggggcaa atgatgaatc
      781 accaccacgc aggtttgtgt tggagttagg gctttgagct gctgcataat atcgatttgg
      841 tagtaggggt cgagggcggc aatcggttca tcggcaagca agatcggggc ttgtttcatc
      901 aaacaacggg cgagttgcac tcgggtctgt tcaccgccgg agagctgttg gtaagtgttg
      961 tccagcaggt gagaaatcgc caattgttcg gcaaccgaac gcaccttttg acgctcttgt
     1021 tcaggcggaa gcggtgcggg caagccgagt gcaatcacat catacaccga aagttgccac
     1081 gccacgttga gctgttgggc gaggtaggca aattgtcggc tcctctcaac ggcggaaagg
     1141 gcgtgcagtt tgcaattttc cagccaaatt tcaccgcttg tcgggcgtag aatgccggca
     1201 atcgctttga gcaacgagga tttccctgcc ccgtttgcgc ccataatgcc gatcaatttg
     1261 ccttttggca gatagcaaga gaggtgttga ataccgtggg caagggtgag attttcaatc
     1321 ttgatcattg tgccaatctc cattgctggg tgaataaaat ccaaatcaaa aacggtacgc
     1381 ccaaaattgc cgtgagcgtg ccgatgtaaa ttcgggcaaa aaacggcaca tattgcaccg
     1441 ccagatctgc aatcagcagt aacaacgccc cgattaaagc ggaggtaaga taaagctggg
     1501 acggcggttt tttcagccaa atacgggcaa gatgaggggc tatcaagccg ataaaaccga
     1561 tggtgccggt ctgtggaatc gttgctccga ccaacagcgc cacgccaatg ctaacggtaa
     1621 aaaaactgcg ttgcaggttg atgcccattg ttgctgccgt ctcttcgcca aaggtgagca
     1681 ggtcgagata acgccgttcc cgatagaggc agacaatgcc gagcaagatc aacggtaacg
     1741 cccaacatac tgcctctgcg gttgcccaga ctaatgagcc ttgcaaccag cggtagagtt
     1801 ccgccaacgc ccacgggctt tcggcattgg aaagcagtaa agtcaccgct gccccgagca
     1861 acacgtttac cgccacgcca cttaaaatca tcattgttgt gccgtggcga aagctaatca
     1921 gccacaccag caaaaatgcc cccagcgccc ctgccacacc gccaaacagt agccagccca
     1981 tcggggctga aaaatagtag agcagaaata cgctcaccgc cgttgccccc gaactgctcc
     2041 caagcaaacc ggggctggca agcggatttt taaacacgcc ctgcatcgca ttgccggcaa
     2101 gcgcaaggct ggcgcccgtc agcaccgcca agccaatacg gggcagtcga atttcaccca
     2161 gcaccagcca gcgcagatcg cttagcacgc cttccgcatt tttaagcaag gcaaaatcgc
     2221 tcagttggta aaaggctgcg ttgctaacaa tcagcaacaa caagcttcca agcacaatgt
     2281 ttaatttctt cattcttctt tctctcatca aacgtgcaaa ttataccata gcgccaaagt
     2341 caagctgaaa acgctatttt tgcgatcatc atcgaaaaac tagcgtttgc cgaatgtaac
     2401 ccacgacagg ctttgctata ttccttcaat cagcctaagt tttgtattta aaacattaca
     2461 gattgggggt agaatgtacc atattgaacg cactgaaatt ttcgaccaat ggcttgaaaa
     2521 tttaaaagat ccacttgctg tggtgaccat tgcaagacgt attgaacgtg ccgaaagcgg
     2581 aaattttggc gataccaaat atatcgcaga aggaatttcc gaaatgaggc taaacgtcag
     2641 caaaggttat cgcctttact atgcgcaaaa aggtagcatt atttatatac tactaaatgg
     2701 cggacataaa ggcacgcaac aagctgatat tgataaagct aaaatgctat gggcagaaat
     2761 taaacaacat atggcagaac aggagaaaaa gaatgaacga attatatgac gccccaattc
     2821 cacaagctac tcgtgaaaaa tttggcattc aacctttcga tgccgctgaa tacctcgcca
     2881 atgacgagct aatcgccctt tatttggcgg aaacattgaa agacggcacg gacgaggagt
     2941 ttattcaagc attgaatacc gtggcacgag cgaaaggaat gaatgagtta gcgaaaaaag
     3001 cagggatcgg gcgtgaaagt ttgtatcaaa gcctttccag cagtaagccc cgttttgaga
     3061 ctatccgcaa aattattagc gcattaaatt tggagttgtc ggtcgttaag gcgtaagttt
     3121 gtgcattaat tgtatcccct gccaaacccc gtggtcgaaa cagtaggtgt atttcatcgg
     3181 taggacaagc ggtggatttt ggctcatttt ttgccatatt ttttgtaatg ctgggtgttt
     3241 gagcagttcg gcttggttgt tatagctttg gttatcggtg aggaacaata actgtttggg
     3301 ttgcgctaac agcagttttt ctagcgagaa attctgtggc gtgaggtcgc tttttaatgg
     3361 tgacagaccg agaagatcca acaacgtttg atattgcgga aaatgcggtt cgatcatacc
     3421 cgtttcgctt agaattaaag tttcagcaag cggttgtttt agcggaattt tttgcaaacg
     3481 tagccgttcc accagctttt ccgctttggc ttgattttgg gttagctcgc ccagttgtaa
     3541 aatcagtgtg aaaagctgtt cgggtgtttg cggactgtcg tttaatgcga ctaacttaaa
     3601 gccaagctgt ttcagccgtg cggtgagttg cggatagaag gtttcattca gcaaaatggt
     3661 gctgtcggca tagggcaaca gggcggtgag ttcaggctca acggtgggct tgtccgtgtt
     3721 cagtttatcc aacatcatca acggcttttg ggaatagggc gacatcgccc gaatttgctg
     3781 tggttcggca agttccagca acagacggtc gctacacaaa tttggcgata aacggtaagc
     3841 tcctgaaata cgcccagtga tagaattttt aaactgtgaa ttatcggtgt aacggccact
     3901 aatagaaaaa ctgtgatctt gttcggtaaa taagcgatat tctagtgccg cacttttttc
     3961 ggataaggtt ttttcacgca aatatttatc agaggtatag cgtgtttttg cataatctgt
     4021 cagtaaactg accgcttgtg tcacttctcc ttcacggtca aagtttacat ctaattgata
     4081 attcgcatta agttgttttt cgttgctgta gcgagcattt gaaccgaaat gtgttgcttt
     4141 actattgaat tggctgatat ttgcttggtg tttcagcagc tcttgttcat ttccccaata
     4201 accgcctaat ttaaaagttt gattgcgagt acgggaatat tcaccgctaa cgtctgaatt
     4261 atagccatca atatgaacgg tttgggaaga ttgagcggca agaacttcta cccctttgtt
     4321 accgaggtca taacctaaac gtaaagagcc gttatcacga tgaaagccat ctcgttcgct
     4381 tgcaccaccg gttttcactt ctgagcctgt ttctgtggta tagctgaaat ggtttttact
     4441 cagtgcggaa atgcctttgg tacgatggct atcaccgtgt aaggcatagt agaaaccgtt
     4501 gtgaaatccg gaaagtgttg cgctggcatc acgggtatta tttgagccta aaccaagatc
     4561 gacctccgca ttgaatggtt tctctttgta taaaccgctt ttagtggtga tataaactac
     4621 accacccata gcactgcttc cccaaagggc ggattgctcg ccacgcaaca cttcgatacg
     4681 ttcgatattg ctaatcggta agccaccaaa atcgaaattt ccaatattaa tcggattgac
     4741 ttttacaccg tcaatcacaa ccgcagtatg gcgagattct gcccctcgta aaaataagct
     4801 agtaagagta ccacgcccac cttgttgccc aatcgccacg cccggcacag tctttaacac
     4861 atcactcaca taagtcgcat tgcgtgcggc aaaatctttt tcggtaagaa ccgtcacgct
     4921 ggaagcggtt ttgtcttggt tgattggggt tgcataggcg gaatagactg ttacggaatc
     4981 aagctgagtc atctcatttt gtgcaaaagc tgtacttgct agtgcaacag aaatggcgaa
     5041 tgctgtggga ttctattgat cgcctaagtc ctttaaaacc gaaatttgtt tcggtaactt
     5101 atggagcaaa ttctggtgag cgagatcgta cacacagtat tgtgaaatca atccaagaaa
     5161 aaacagggat catttcagct ccgcacttaa caggcattga tgcaacaccg gaagaattaa
     5221 aggtaattgc caaagattat tgggatagtg gtattcgccg aattgttgct ttgcgtggcg
     5281 atataccgaa aggttatgat aaagcactat tttacgcctc agatttagtc gctttattaa
     5341 aatcagtagc agatttcgat atttccgtgg ctgcctaccc tgaagttcac cctgaagcaa
     5401 aatcagcaca ggcagattta cttaacttga aacgtaaaat cgatgcgggt gcaaaccacg
     5461 ttattacgca attctttttt gatgtagaga gttatttacg ttttcgtgat cgctgtgttt
     5521 ctattgggat tgattgtgaa attgtgccgg gtattctgcc ggtgacaaat ttcaagcagt
     5581 tgcaaaaaat ggcagcagtg actaatgtta agatcccgac ttggatgggt aaaatgtacg
     5641 aagggttaga tgatgatcaa accaccagaa acttagttgc agcaagtatt gcaatggata
     5701 tggtgaaagt gctttctcgt gaaggggtga atgatttcca cttctatacc ttaaatcgta
     5761 gtgaactcac ttatgcaatg tgccatactt taggcgtaag acctgattta acaactgcat
     5821 aatcgaacat taaggctgta tagctaaatg agctatacct ccttatagtt ttacttctct
     5881 tcagccaccc aaatctcgcc attttttaat aataaatagg ctttgttgga atcggctttg
     5941 gtttgtttaa ttccagccgg cagggcggtt ggcaattcag tgatcttgcc tgttttatcc
     6001 aacacaacaa cttgttgata gcccatttgg ggtatcagtg cgaaagtctc tgttgtcgcc
     6061 actaaattgg tttcgtgatc accatagcgg tgattgctta agccggaggt aataaaacgt
     6121 tcagctaatt ggtttccttt caggctgtat tcaaccaaat tgccttttag atgtggcatt
     6181 tggattgcat agagcttgcg gttaaagata aacggcgatt gccaacggtt agtcgacagc
     6241 ggctcacttt cggcttctat tgataatttt ccgtctttaa cgccaatcac aacagtgctg
     6301 gccccattgc catcgccaga gataacagaa acgatcttgg tttttccatt ttctgtgaaa
     6361 gtcgcccagc ggttggcttc aaatactcgc ccttgttggg tgagtttttc tgctaagggg
     6421 tttagggtat ggcgttctag aaattgaatt tcgcctgcct ctacatcatc gcctaataca
     6481 ccgtgttgat atgtaaaatc atcaggcttt gcaagtacag caatatgccc ttggttaggt
     6541 gtttcggcat caaaatcaat ttgcagaggg tgagcatcag gcaacacctc ggtttctgcg
     6601 gtggcagtaa tagtggcttg gtttccttgc gtttcaattc tggctaactt aaattgtccg
     6661 ttttgtttgg tcacggcaat ggtggcaaaa ttaagggcgg tcattgtagc tttcggggca
     6721 aggggaatgt tggaggtata gattttatca gccgtccaca gcataaaatg cccgttttga
     6781 tccgctaacg caattcgttg gtattgagct ttaggttcaa tctcggtgga aactccatct
     6841 gccagttttt gagcttgttg tgtgactctc cataattgtt tttgttggtt aattaccact
     6901 aattcgcctt gttctgaaag gtcaaattgg cttactttgc caatttgggg aagtgggagt
     6961 ggagaaagct gaacatcagc aaacaaagaa gcggagaaca agcttaagat taacagaata
     7021 gattttttca ttagcgttac ctaaaaagaa gatttaaatg ataaagggag atgttatctc
     7081 cctttacgct ttagccttta taaccattcg ggttattttt ttgccaattc caagtgtctt
     7141 tcatcatttg ttctagccca cgctcggttt cccaacccag ttgctctaac gctttttgtg
     7201 gagcggagta gcaaacggca atatcgcccg ggcggcgatc taccactttg tatggaatag
     7261 taatgccatt tgcggcttca aaggctttta ccatatctag caccgaataa cctgttcctg
     7321 tgcctaaatt gtaaacgtgg aaaccggcat cattttggtg cttgtctaag gcttttaagt
     7381 gaccaagtgc taaatctacc acgtggatat aatcacgcac acctgtgcca tcgtgagtat
     7441 tataatcgcc accaaacacc gataattgag gtaatttgcc cacagccact tggctgataa
     7501 acggcattaa gttatttggg ataccgtttg gatcttcacc gattaaaccg ctttcgtgag
     7561 cccctaccgg gttgaagtaa cgcaatacta ccgcacttaa ttgtgggaag gctttaacgg
     7621 tatcttcaag aatgcgttca accatatatt tggacgtacc gtaagggttg gttgtgccac
     7681 cgacagggca agattccaca atcgggataa tctgcggatc accataaacg gtcgccgatg
     7741 aactaaacac aatggtattc acattggctt ttagcatctc ttctaccaat acaatcgagc
     7801 cggttacatt gttttgatag taacgtagcg gttctctgac gctttcgcct acagctttta
     7861 aaccggcaaa gtgaattacc gattcaatct gattttccgc aaaaattttg cgtaaaatat
     7921 cacgatctaa aatatcgcct tgatagaatt ttacgctctt gccggtgatc tgcttcacac
     7981 gctcaagtga tacttcagag gaattagaaa gattatctaa caccacgatt tcacgatttt
     8041 cgtttaataa ttcaactaat gtatgtgaac cgatgtagcc tgcaccacct gtaactaaaa
     8101 ttgccatttt atgttcctct ataaaattaa tacgaaccaa ggttattgtt gctattttac
     8161 ccgatttaat caggcttgct aatatttatt cagtttataa tgccactccg gcttccggca
     8221 gtaattccgg aatgccgttt ttaatcgggt aagcaatatt gagcttaggg ttaattaatc
     8281 ggttgttctc tttgtcccat tctaattttt cattagagac agggcagacg aggttattga
     8341 gtagtgtttc gttcatcttc ttttcctagt ttttgtagaa tcggggctaa caaacaagcg
     8401 gtcgattttt ctgaaaatgt tgcagaaacc ggtacatacc accaatttgg ctgtgcaaaa
     8461 tcgtggcatt ttaccgcatc tttctcggtc attaataacg gcgtttggct atcagcaaat
     8521 tgagccagct gagccttatc aaaggcttga tgatcggcaa agccttgagt tttggcaagc
     8581 ggaatgccta atttttctaa catcgtaaaa aagcgtggtg ggtgaccgat accggcaatc
     8641 gccacacagg gttgggtaaa ttcgcttaat ggcttgcgtt gattggtgac taaattaatt
     8701 gcatcagacg gttcaagcgt cattaaatgc tcgttagctt gcggtgtgcc gccgttgcaa
     8761 ataaccgctt gcacggagtt taaacggctc ggtaattcac gcaatccgcc cgccggcagc
     8821 acaaaaccgt taccgaaacg ccttacgcca tcaaccacca cccattccat atctcgctgt
     8881 agggcatagt gttgcaagcc atcatcggtg atgatgatgt ccggctcaaa ctgatttaat
     8941 agtaattcaa tgctttgttg gcggtttgga gaaatagcca aaggcacatt ggttcgctga
     9001 acaatcagaa ccggttcatc acccaccatt tcagcagaac tttctcgggt aactaattgc
     9061 ggaaacgttt tcgctttacc gccatagcct cgagaaatca cgcctacttt tacgccacgc
     9121 tgttgcagtt gttccaccag ccaaacgacc actggcgttt tgccgttgcc tccaaccgag
     9181 atattaccga ctaccagcat aggcaaaggc gaacggtacg attgcaaaat attcagagaa
     9241 tataaccgct tgcgagcttg actaatcagc caaaacagca acgaaagcgg cgaaagcagc
     9301 caagtgataa gggattttgt ttgccagatt ttcatattat ttatttttaa gtgtttcaac
     9361 taaatctcgt aataaggcta attcacgttt ttggttttcg ataatttctt ctttatgggc
     9421 taataattca tctttgtggg agataataag ttgtaaacgt tgaatttctt ctactccatt
     9481 tcct
//
LOCUS       DANLXF010000088         9392 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.088, whole genome shotgun sequence.
ACCESSION   DANLXF010000088 DANLXF010000000
VERSION     DANLXF010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9392
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.088"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(17..1306)
                     /locus_tag="PXD66_001365"
     CDS             complement(17..1306)
                     /locus_tag="PXD66_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDL6319662.1"
                     /translation="MDSIPLSSLFITLAILLVLSAFFSGSETGLMSLNRYKMRHLAEQ
                     GHKGAKLAEKLLARTDVLLSLILICNNLVNIAASAIATMIGMQLSGEAGVAIATGLLT
                     FVMLVFSEILPKTVAAIYPEKVGFFASFVLTPLKKLLSPIVFLMNLIIAGLMKLLRIQ
                     QKDKQGLSSEELRSVVLDAGKFIPAEHREMLVSILDMEKVTVDDIMVPRNDISGIDID
                     DDWKAIMRQLNHAPHARVIIYKGDMDNNIVGILRVREAFRLMLEKNEFTKEMLLRATD
                     EVYFIPEGTPLTTQLMNFKANKERIGLVVDEYGDIKGLVTLEDILEEIVGEFTTSSAP
                     TLEQEVQQQSDGTVIIEGSANLRDLNKLFDWDLPLEEARTFNGLILEHLEKIPDEGME
                     FELYNLKVMILEVSENMVKQAKVEHLSPKIEKEQDSA"
     gene            complement(1430..2227)
                     /gene="ccsA"
                     /locus_tag="PXD66_001366"
     CDS             complement(1430..2227)
                     /gene="ccsA"
                     /locus_tag="PXD66_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HDL6319663.1"
                     /translation="MLLPLLSIVAYALALLWVAPTLVNLENAATVNKKPNMMLVFGFG
                     LLATILHFSVLYNELFLIEGQNFTVTNVISLMSFIMAACVTLALLKWKTVWFPLIIVY
                     SFAICSVGVAGFVKGSFIRNVQENAGLLFHLGIALFSYALFFLALIYAFQLKWLDRKL
                     KSKTMFFCQILPPLMTVERHFFTLTLAAQAMLTLTLFSGVVYLHNFFEPEHIHKAIFS
                     FLAWLVYSIQLLGQWKLHWRGKRVLIYSISGMMLLTIGYFGSRLIVN"
     gene            2443..2967
                     /gene="yceD"
                     /locus_tag="PXD66_001367"
     CDS             2443..2967
                     /gene="yceD"
                     /locus_tag="PXD66_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005621677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HDL6319664.1"
                     /translation="MQKVKLPLTIDPYKDAQRRVDYEGYISGKLLTRLGESVCNVQSD
                     AQVTLSLYVDPQRLTVIKGTAKVDVEFDCQRCGDPFTQTLDCSFVFSPVSNMDQADIL
                     PEIYEPIEVNEFGEVNLLDMIEDEFILELPLVPMHSEEHCEVSVSEQVFGELPEELVQ
                     KPNPFAVLANLKKN"
     gene            2984..3154
                     /gene="rpmF"
                     /locus_tag="PXD66_001368"
     CDS             2984..3154
                     /gene="rpmF"
                     /locus_tag="PXD66_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006231206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDL6319665.1"
                     /translation="MAVQQNKKSRSRRDMRRSHDALTTAAVSVDKTSGETHFRHHVTA
                     DGYYRGRKVINK"
     gene            3173..4195
                     /gene="plsX"
                     /locus_tag="PXD66_001369"
     CDS             3173..4195
                     /gene="plsX"
                     /locus_tag="PXD66_001369"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDL6319666.1"
                     /translation="MVDITLAVDVMGGDFGPRVTIPALEKALKQNPSLSFLLFGDEHQ
                     IAPLLQKHTLLNHPKIQIHHTEQVIEADVPFTTAIRQSKNSSMRLAIEAVKEGKAQGC
                     VSGGNTGALMGLAKLLIEPLPNIERPALTSLIPTLNGKSSVMLDLGANIEVSNHQLQQ
                     FAEMGNLFAQVMLDLVYPRLSLLNIGTEENKGTAQLQAVHQLLKNRQDLNYTGFIESD
                     KLTSYLTDVIICDGYTGNIALKALEGAAKNIISLFKKEKADSNICRNTKRYLLKAMFY
                     RYYRKLQQINPDRHNGATLLGLSKVVVKSHGGANINAFYYAIDYAVQQIQGDIPNKIL
                     EGLTQK"
     gene            complement(4292..5575)
                     /gene="hisD"
                     /locus_tag="PXD66_001370"
     CDS             complement(4292..5575)
                     /gene="hisD"
                     /locus_tag="PXD66_001370"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDL6319667.1"
                     /translation="MQTLIWNNLTETEQQQYLARPAQVVGESIKAAVNEIKANVIANG
                     DKALFELAEKFDKVKLESLVVSEQQIQQAQSRISPELKQAIQNAKANIEAFHKAQQNQ
                     EVDIETQPGVCCQVITRPINAVGLYIPGGSAPLFSTVLMLAVPAKIAGCNKIVLCSPP
                     PIADEILYTAHLCGVETIYAVGGAQAIIAMANGTQTVAKVDKIFGPGNAFVTEAKRQV
                     VQEGTAIDMPAGPSEVLVIADEFADPAFVASDLLSQAEHGADSQVILVTPSESLARAV
                     DEELEKQVAVLPRAETARKALAHSRTFIAENLQQAVEISNKYAPEHLIVQTENARSLL
                     ADLDNAGSIFLGAYSPESMGDYASGTNHVLPTYGYTKTYSSLGLADFSKRMTVQELTP
                     QGFLALAETVERMASAELLDAHRNAVSLRVAKLKG"
     gene            complement(5587..6486)
                     /gene="hisG"
                     /locus_tag="PXD66_001371"
     CDS             complement(5587..6486)
                     /gene="hisG"
                     /locus_tag="PXD66_001371"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDL6319668.1"
                     /translation="MTTQNRLRIAMQKKGRLSQECSELLKQCGVKIIWNDQRLIAYSE
                     NMPIEILRVRDDDIPGLVFDGVVDLGIIGENVLEEVELGRLSAGENVAYKKLRQLDFG
                     GCRLSLAIDRDKNYNNIKDLEDAVIATSYPNLLKRYMKEQNVPFKSILLTGSVEVAPS
                     AGIANAICDLVSSGATLEANGLKEVEVIYRSTACLIQRQEPLSAEKQALVDKLLTRIQ
                     GVQQANESKYIMLHAPKDKLAEITALLPGVENPTILPLANDQTRVAMHVVSQENLFWE
                     TMEQLKEMGASSVLVLPIEKMLA"
     misc_feature    complement(6519..6646)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            6845..7702
                     /locus_tag="PXD66_001372"
     CDS             6845..7702
                     /locus_tag="PXD66_001372"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDL6319669.1"
                     /translation="MQISLKKIYSGKVRDLYEIDEKRMLMVATDRLSAFDVILDDPIP
                     RKGEILTQISNFWFNKLAHIMPNHFTGDSVFDVLPQEEAEAIQHRAVVCKRLIPVKIE
                     SIVRGYLTGSGLKDYQQTGTICGLTLPEGLVEASKLPQPIFTPSSKAEVGDHDINISY
                     EECERQIGQELAAKVRDAALKLYTEAANYALTKGIIICDTKFEFGLDENGELTLMDEV
                     LTPDSSRFWSVETYREGTNPPSFDKQFVRDWLENSGWNKQPPAPKIPAEVIEKTVAKY
                     QEALDLLTK"
     gene            complement(7843..9324)
                     /gene="ilvC"
                     /locus_tag="PXD66_001373"
     CDS             complement(7843..9324)
                     /gene="ilvC"
                     /locus_tag="PXD66_001373"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDL6319670.1"
                     /translation="MANYFNTLNLRQKLDQLGRCRFMDRDEFADGCNFLKGKKIVIVG
                     CGAQGLNQGLNMRDSGLDIAYALRAEAIAEKRASFKRASENGFVVGTYEQLIPEADLV
                     INLTPDKQHSKVVADVMPLMKQGAAFGYSHGFNIVEEGEQIRPDLTVVMTAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGLAIAKAWASATGGDRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSIVCYDKLVSEGKDPAYAGKLIQYGWETITEALKQGGITLMMDR
                     LSNSAKLRAFELSEQIKEELAFLFEKHMDDIISGEFSSTMMADWANGDANLLKWREET
                     GKTAFENAPQADGIKISEQEYFDNGVLMVAMVKAGVELAFDTMVSAGIYEESAYYESL
                     HELPLIANTIARKRLYEMNVVISDTAEYGNYLFSHVATPILAEKIIPTLEKGDLGEPT
                     PAVEIDNVTLRDVNDAIRNHPIELIGQELRGYMTDMKRISSQG"
BASE COUNT         2785 a         2132 c         1775 g         2700 t
ORIGIN      
        1 ttcttttttc ttaaaattag gctgaatctt gctctttttc aattttcggt gaaagatgtt
       61 caactttcgc ttgtttaacc atattttcac tcacttcaag aatcatcact ttcaagttgt
      121 agagttcaaa ttccattcct tcatcaggaa ttttttctaa atgctctaaa atcaagccgt
      181 taaaggttct ggcttcctca agcggtaaat cccaatcaaa tagtttgttc agatcacgca
      241 ggttggctga gccttcaata atcaccgtac catctgattg ttgctgaact tcttgctcaa
      301 gggtcggtgc tgatgaagtc gtaaattcac ccacaatttc ttctaaaata tcttcaagag
      361 tgactagccc tttaatatca ccatactcat caaccactaa accaatacgc tctttattcg
      421 ctttaaagtt cattaattgt gtagtgagtg gtgtgccttc aggaataaaa tagacttcat
      481 ctgttgcacg cagtagcatt tctttggtaa attcgttttt ctccaacatt aaacgaaagg
      541 cttcacgcac ccgcaaaatc ccaacaatat tgttgtccat atcccctttg tagataatca
      601 cccgagcgtg aggggcgtga ttaagctgac gcataatcgc tttccagtca tcatcaatat
      661 caatgccgct gatgtcgttg cgaggcacca taatgtcatc aaccgtcact ttttccatat
      721 ctaaaataga gaccaacatt tcacggtgtt cggcaggaat aaatttaccg gcatcaagca
      781 ctacactacg cagctcttcg gagcttaagc cttgtttatc tttttgttga atccgcaata
      841 atttcatcag cccggcaata atcaaattca ttaaaaatac aatcgggctg agtaattttt
      901 taagcggtgt tagcacaaag ctggcgaaaa aaccgacttt ttcagggtaa attgccgcta
      961 ctgttttagg taggatttcc gaaaaaacca acattacaaa agttagcaaa ccggttgcaa
     1021 ttgccacacc ggcttcaccg gataactgca taccgatcat cgttgcaatc gcagaggcgg
     1081 caatgttgac taagttatta caaattaaga taaggctaag tagcacatcg gtgcgtgcca
     1141 agagtttttc cgccagtttc gcccctttat gcccttgctc ggcaaggtga cgcattttgt
     1201 agcggtttaa agacatcaag ccggtttccg agcctgaaaa aaatgcagag agtactaaaa
     1261 gaatagctaa ggtaataaat aaactactca ggggaatact gtccaaaatt caattcctat
     1321 gtaaattaat cataaaaggt agcgtaacac taaaagcgtt gtcactacct cactttgtcg
     1381 gttcagccga cttgcaaaaa attttgcttt tttgaccgct tgtagatcat taattgacga
     1441 tcaaacgact accgaaatag ccgatggtga gcaacatcat acccgaaatc gaataaataa
     1501 gcacccgttt tccacgccaa tgtaatttcc actgtccaag taactgaatg ctataaacaa
     1561 gccaagctaa aaaggagaaa attgctttat gaatatgctc cggctcgaaa aagttatgca
     1621 aataaaccac accggaaaat aaagtgagcg tcagcatagc ttgtgcagct aaggttaaag
     1681 taaagaaatg gcgttcaacc gtcattagcg gtggtagaat ctggcaaaag aacattgttt
     1741 tgctctttag ttttctatcc agccatttga gttgaaaagc ataaattaat gctaaaaaga
     1801 agagagcata agaaaataat gcaatcccta aatggaaaag cagtcccgca ttttcttgca
     1861 cattacggat aaaactacct tttacaaatc cagccacccc cacactacaa atggcgaaag
     1921 agtacacaat aattaaagga aaccaaaccg ttttccattt taacaacgca agtgtgacac
     1981 aagccgccat aatgaaactc atcaaagaga ttacatttgt taccgtaaaa ttctgccctt
     2041 cgattaaaaa taattcatta tataacacag aaaaatgcaa aatcgtggca agtaacccaa
     2101 acccaaagac taacatcata ttaggctttt tattaactgt tgcggcattt tctaaattca
     2161 ctagagtagg ggcaacccaa agtagcgcta gagcgtaagc aacaatggat aaaagaggaa
     2221 gtaacatatt gtctcgaatc tcgtcaaata agaataacta tcagaatttt aatcaaaagt
     2281 aaccgtttta gctagttttt gctgttgcta gatcaaaaat tatcctaaaa aatgtgcttt
     2341 tcgccttaaa ttgaacccta ccttttctcg gtaagattat ctcactgaaa acaataattt
     2401 tcctttgact aatttgcttt ctatctgtaa tatgcacacc ctatgcaaaa ggtaaaacta
     2461 ccccttacga ttgaccctta caaagatgct cagcgtcgcg ttgattatga aggctacatt
     2521 tcaggtaaac ttctaacacg cttgggtgaa tcagtatgta atgtgcaaag tgatgcacaa
     2581 gtcactctct cgttatatgt cgatccacaa cgcctaactg tgattaaagg cacagcaaaa
     2641 gtagatgtgg aatttgattg ccaacgatgt ggtgacccgt ttacacaaac gctcgactgt
     2701 tcatttgttt tcagtccggt gtctaacatg gatcaggcgg acatcttgcc cgaaatttat
     2761 gaacctatcg aagtaaacga gttcggtgaa gtaaatttac tagatatgat tgaagatgaa
     2821 tttattcttg aattacctct agtcccgatg catagtgaag aacactgtga agtgtccgta
     2881 agtgaacagg tatttggcga gttgccggaa gaactggtac aaaaaccaaa cccgttcgca
     2941 gtattagcta atttaaagaa aaactagatc taggagaaag cccgtggctg ttcaacaaaa
     3001 taagaaatct cgttcacgtc gtgatatgcg tcgttcacac gatgcattaa caactgcagc
     3061 agtttcagta gataaaacca gtggtgaaac tcactttcgc caccacgtaa ctgcagacgg
     3121 ttactaccgt ggtcgtaaag taatcaacaa gtaattcctt tcgaggttta tcttggttga
     3181 tatcactctt gcagtagatg tgatgggcgg ggactttggt ccccgcgtta ctattcctgc
     3241 actggaaaaa gccttaaaac aaaatccaag cctttctttt ttattatttg gtgatgaaca
     3301 ccaaatagcc cctttattac aaaaacacac actactcaac caccccaaaa tccaaattca
     3361 tcataccgaa caagtgattg aagctgatgt tccgttcact actgcaatcc gccaaagcaa
     3421 aaacagctct atgcggttag cgattgaggc agtaaaagag ggcaaagcac aaggttgtgt
     3481 tagtggcggt aacactggtg cgttaatggg attggcaaaa ttattgattg aaccattacc
     3541 aaatattgaa cgccctgcac tcacttcgct aattcctacc ctcaacggca aatccagcgt
     3601 aatgttagat ttaggggcaa atattgaagt aagcaatcac cagctacaac aatttgcaga
     3661 aatgggaaac ctgtttgctc aagtaatgct tgatttagtt taccctcgcc tgtctttatt
     3721 gaatattggt acagaagaaa ataaaggcac ggcacaactt caggcagtgc atcaactgct
     3781 gaaaaacaga caagacttaa attacaccgg ctttattgaa agcgataaat taaccagcta
     3841 tctcactgat gtgattattt gtgacggcta caccggcaat atcgcgttaa aagcgttgga
     3901 aggggcagct aaaaatatta tttctctctt taaaaaagag aaagccgatt ccaatatttg
     3961 ccgtaacacc aaacgatatt tactgaaagc gatgttctac cgttactacc gcaaattgca
     4021 acaaattaac cctgaccgcc ataacggtgc gaccttgctc ggcttatcta aagtagtcgt
     4081 gaaaagccac ggcggggcaa atatcaatgc cttttattac gctattgatt atgcggtgca
     4141 acaaattcaa ggcgacattc caaataaaat cctagagggt ttaacccaaa aataagcgaa
     4201 ctcaactgtt cgcttatttt catttcttgt ttcacttaaa gatacaagcg gtcaaatttg
     4261 caaaaaaatt tacccattca accgcttgtg tttagccttt taattttgcc actcgtaaac
     4321 taaccgcatt gcggtgtgca tctagcaatt ctgcacttgc cattcgttcc accgtttcgg
     4381 ccaaggctag aaagccttgt ggtgtcagct cctgcaccgt cattcgcttg ctaaaatcag
     4441 ctaaacctaa gctcgaatag gttttagtgt agccgtaagt cggtaaaacg tggttagtgc
     4501 cgctagcata atcccccata ctttccggcg aatacgcccc taagaaaatc gagccggcat
     4561 tatctaaatc cgccaacaac gagcgagcat tttcagtttg cacaattaaa tgctcgggtg
     4621 catatttatt actaatttcg accgcttgtt gcaggttttc cgcaataaaa gtgcgactgt
     4681 gagccaatgc tttacgggca gtttcggcac gaggtaaaac cgccacctgc ttttctaatt
     4741 cttcatcgac tgcccttgcc aagctctcac ttggtgtgac taaaatcact tgggaatcgg
     4801 ctccatgttc tgcttgagag agtaaatcgc tcgccacaaa tgcagggtca gcaaactcat
     4861 cggcaatcac caacacttcc gacgggcctg ccggcatatc aatcgccgtg ccttcttgca
     4921 caacttggcg tttcgcctcc gtcacaaacg cattgcccgg gccgaaaatt ttatccactt
     4981 tcgctaccgt ttgtgtgcca tttgccatcg caataatcgc ttgagccccc ccaactgcgt
     5041 aaatcgtttc caccccacac aaatgggcgg tgtagagaat ttcatcggca atcggcggtg
     5101 gtgagcaaag cacaatcttg ttacaaccgg caatttttgc cggcactgca agcattaaca
     5161 cggtagagaa aagcggggct gaaccgcccg gaatataaag ccccaccgca ttaatcgggc
     5221 gagtgatgac ttggcaacac acccccggct gggtttcaat atccacctct tgattttgct
     5281 gtgccttgtg gaaagcctcg atattcgctt tcgcattttg aatcgcttgc ttaagctctg
     5341 gtgaaatacg gctctgtgct tgctgaatct gctgttctga tacaactaag ctctctaatt
     5401 ttactttatc gaacttttca gctaattcaa ataatgcctt atcgccgttg gcaattacat
     5461 tcgctttaat ttcattaact gccgctttaa tactctcgcc aaccacctgt gccggacggg
     5521 caagatattg ctgctgctca gtttctgtta aattgttcca aattagggtt tgcatattta
     5581 ttccttttac gccaacattt tctcaatcgg taaaaccaat acggaacttg cgcccatttc
     5641 ttttagctgc tccattgttt cccagaacag gttttcttgg ctgacaacgt gcattgccac
     5701 acgggtttgg tcgttagcta acggtaaaat agtcgggttt tccacaccgg gtaataaagc
     5761 ggtaatttcc gccagtttgt ctttgggggc gtgcagcata atatacttag attcgttcgc
     5821 ctgttgcaca ccttgaatgc gggttaataa tttatccact aacgcttgtt tttccgctga
     5881 aagtggttct tggcgttgaa ttaggcaagc agttgaacgg taaatcactt caacctcttt
     5941 taatccgttc gcctctaatg ttgcacctga agaaaccaag tcacaaatcg cattcgcaat
     6001 tccggcactt ggagcaactt caaccgaacc ggtgagtaaa atacttttaa acggcacatt
     6061 ttgctctttc atataccgct ttaataaatt ggggtaagaa gtggcaatca cagcatcttc
     6121 taaatctttg atattgttat aatttttatc acgatcaata gctaacgata aacggcagcc
     6181 accaaaatct aactgacgaa gttttttata ggcaacattc tcaccagcac ttaatcgccc
     6241 taattccact tcctctaata cattttcacc gataatccca agatcaacta cgccgtcaaa
     6301 aactaagcct ggaatgtcgt catcacgcac ccgtaaaatt tcaatcggca tattttctga
     6361 ataggcaatc aggcgttgat cgttccaaat gattttcacg ccacattgtt taagcaactc
     6421 actacattct tggcttaagc gacctttttt ctgcatcgca attcttaatc gattttgtgt
     6481 tgtcattgtt gtttccttct tatgttttaa attgtaaaaa taaaaaaacc tctcgaaatc
     6541 acttccgaga gggttaaatt tatgctgcgt ttaggcgtac tcggaagtct tatcttccgc
     6601 acataccaaa tgcccgaaag attattcagg taaatgatgg tggtgatgag tacgaataaa
     6661 agccattttg ttgttctcca aactaaattg ctttaaaaga tacgcttaaa ttttggcaat
     6721 gacaagtgtt ttttgcgaaa aaattgctat ttttcatctc tctaaaacaa ctatcgcaaa
     6781 cgtttgcgta aagcggtata atttgcctga ttttttgcaa attcaacaaa gtaggaataa
     6841 cactatgcaa atcagcctca aaaagatcta ctccggcaaa gtccgtgatc tctacgaaat
     6901 cgatgaaaaa cgtatgctta tggttgccac cgaccgatta tctgcctttg atgttatttt
     6961 agacgatcct attcctcgta aaggcgaaat cctcacccaa atctccaact tctggttcaa
     7021 caaattagcc cacattatgc caaaccactt caccggcgac agtgtgtttg acgtgttacc
     7081 gcaagaagag gcagaagcta ttcaacatcg tgcggtggtt tgcaaacgct taataccggt
     7141 gaaaattgaa tcgattgtgc ggggttattt aaccggctcc gggctgaaag attaccaaca
     7201 aaccggcacg atctgcggct taacattgcc ggaaggcttg gttgaggcca gcaaattacc
     7261 gcaaccgatc ttcacaccgt ccagcaaagc cgaggtgggc gatcacgata ttaatatcag
     7321 ctacgaagag tgtgaacgcc agatcggtca agaacttgcc gccaaagtgc gtgatgccgc
     7381 tctaaaactc tacactgaag cggcaaacta tgccctgacc aaaggaatta ttatttgcga
     7441 caccaaattc gaattcggct tagacgaaaa cggtgaacta acgctaatgg acgaagtcct
     7501 aaccccggat tccagccgtt tttggtcagt tgaaacctac cgagaaggca caaatccacc
     7561 ttcatttgat aaacaattcg tgcgtgactg gctggaaaat agcggttgga acaaacaacc
     7621 cccagccccg aaaataccgg cagaggtaat tgaaaaaacc gtagcgaaat atcaagaagc
     7681 attggattta ttaaccaaat aacaccacaa gcggtcaaat ttacgctatt ttttgcaaaa
     7741 caaaaacctg taagccaaca cttacaggtt ttttagttag tggtaaaaag tgcggttatt
     7801 ctaccgcact tttgcaaaat tttgtgtaaa tctgaccgct tgttagcctt gcgaagaaat
     7861 acgtttcata tctgtcatat aaccacgtaa ttcttgaccg attaactcga ttgggtggtt
     7921 acggattgcg tcattcacat cacgtaaggt aacgttgtcg atttccactg ccggagttgg
     7981 ttcacctaaa tcgcctttct ctaacgttgg gataattttc tccgctaaaa tcggggttgc
     8041 aacgtgagag aataagtagt taccgtattc tgcggtatct gaaattacca cgttcatttc
     8101 atataaacgc ttacgagcga ttgtatttgc gattaacggt aactcgtgta gagactcgta
     8161 gtaagccgat tcttcataaa taccggctga aaccattgta tcaaaggcta attcaacccc
     8221 tgctttcacc attgcaacca ttaacacacc gttatcaaag tattcttgct cagaaatttt
     8281 aataccgtct gcttgtggtg cgttttcaaa ggctgtttta cctgtttctt cacgccattt
     8341 taataagttg gcatcgccgt ttgcccagtc tgccatcatc gttgatgaga actcaccact
     8401 gatgatgtcg tccatatgtt tttcaaataa gaacgctaat tcttctttga tttgctcaga
     8461 aagctcgaac gcacgcaatt tggcactgtt tgataagcga tccatcatta aggtgatacc
     8521 gccttgtttt aacgcttcgg taatggtttc ccaaccgtat tggatcaatt tacctgcgta
     8581 agccggatct ttaccttccg acactaattt gtcgtagcac acgattgaac ctgcttgtaa
     8641 cataccgcaa agaatggttt gttcacccat taaatcagat ttcacttctg ctacgaatga
     8701 agattctaat acgcccgcac ggtcaccacc ggtagcactt gcccacgctt tcgcaatggc
     8761 taaaccttcg cctttagggt cattttccgg gtgaacggca attaaggttg gcacaccgaa
     8821 accacgtttg tactcttcac gcacttccgt gcccgggcat ttcggagcgg tcatcacaac
     8881 cgttaaatcc ggacgaattt gctcaccttc ttccacaatg ttgaaaccgt gtgaataacc
     8941 aaatgccgca ccttgtttca ttaacggcat tacatcggca actactttag agtgctgttt
     9001 gtccggcgtt aagttaatga ctaaatccgc ttcaggaatt aattgctcgt aagtacccac
     9061 cacaaagccg ttttcagaag cacgtttaaa tgaagcacgt ttttcagcaa tcgcttcggc
     9121 acgtaaagcg taagcaatat ctaagcctga atcacgcata tttaaacctt ggtttaaacc
     9181 ttgagcacca caacccacaa tcacgatttt tttgcctttt aagaaattgc agccatctgc
     9241 aaattcatca cgatccataa agcggcaacg acctaactgg tctaattttt gacgtaaatt
     9301 taaagtgttg aaatagttag ccatttttta atccttattt gaattaatga gattgatatg
     9361 atgttttgtt tgcacattgt tatagtttga tt
//
LOCUS       DANLXF010000089         9337 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.089, whole genome shotgun sequence.
ACCESSION   DANLXF010000089 DANLXF010000000
VERSION     DANLXF010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9337
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.089"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            26..1513
                     /locus_tag="PXD66_001374"
     CDS             26..1513
                     /locus_tag="PXD66_001374"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDL6319671.1"
                     /translation="MNAENSCIVIFGASGDLTFRKLIPALYNLYKIGRLGEHFSVLGV
                     SRSELTDESFRQKMRDALVKFEKASGEELDKFCEHLYYQAVNTSDAVDYAKLLPRLDE
                     LHDKYQTAGNTLYYLSTPPSLYGVIPECLAAHGLTTEEFGWKRIIVEKPFGYDIETAK
                     KLDVQIHKCFEEHQIYRIDHYLGKETVQNLLVLRFSNGLFEPLWNRNFIDYVEITGAE
                     SIGVEDRGGYYDGSGAMRDMFQNHLLQVLAMVAMEPPAIINANSMRDEVAKVLYCLHP
                     LTEEDVKHNVVLGQYARGTVDGKEVPAYLEEKGVPADSNTETFMAVKCKIDNWRWAGV
                     PFYVRTGKRLPTRVTEIVIHFKTTPHPVFSQNAPENKLIIRVQPDEGISMRFGLKKPG
                     AGFEAKEVSMDFRYSDLSSSSSLLTAYERLLLDALKGDATLFARTDAVHACWKFVQPI
                     LAYKANRGRVYEYESGTWGPTEADKLIARHGKVWRKPSGTMKKKV"
     gene            1653..2351
                     /gene="pgl"
                     /locus_tag="PXD66_001375"
     CDS             1653..2351
                     /gene="pgl"
                     /locus_tag="PXD66_001375"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HDL6319672.1"
                     /translation="MNKVIFPTAQAAVEQIAKEFIEYSHQNRPVHISLSGGSTPKLLF
                     KTLAQAPFNTEIQWQNLHFWWGDDRMVAPTDPESNYGEVQKLLFDHIDIPSENIHRIR
                     GEENVEQELARFSAELTACVPNLAFDWIILGMGTDGHTASLFPHQTDFNDQNVAVIAK
                     YPETGQIRISKTASLIEKAKRITYLVTGASKAEILKEIQTTPAENLPYPAAKIQAENG
                     VTEWYLDSEAANLL"
     gene            2527..3414
                     /locus_tag="PXD66_001376"
     CDS             2527..3414
                     /locus_tag="PXD66_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HDL6319673.1"
                     /translation="MNPIQLIEPNINSKIFTIRGEQVMLDVHLAELYQVDTRTFNQAV
                     KRNEERFPDIFRFQLTENEWLSLRSQFVILNENSGRGQHRKYLPYVFTEQGIAMLSAV
                     LRSEVAVQVSIKIMQAFVQMRHLLQTNSGLVQRLESVERKQLEMSGQLEQVFQSLEGN
                     KPKMQGVFFEGETFNAYQFVTELVRGAKSSLILIDNYVDDTVLTLFTKRSHNVSVTIY
                     TKQISKQLALDLEKYNSQYAHINVKIFDKSHERFLIIDEKELYHIGASLKDLGKKWFA
                     FSKMDLSMSRILEKLAENQ"
     gene            3424..4113
                     /locus_tag="PXD66_001377"
     CDS             3424..4113
                     /locus_tag="PXD66_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HDL6319674.1"
                     /translation="MKTLGILGGMSAESTVSYYLDINRSVNKVLGGNSSAKILISSVN
                     FEEIVQCQKQGNWQAAAEILSEQARLLEQAGAEGILLATNTMHKIADQISSHISVPFL
                     HILDCVAEAISAQGLQKVALLGTKFTMSDNFYKDGLLERGITSIVPDEETQQEIHRII
                     FEELCLGKITESAKAFYLQTIQQLSEQGAEGVILGCTEIGLLINQNDNPLPFFDTAAL
                     HSQMAVEFILG"
     gene            4196..5650
                     /gene="gnd"
                     /locus_tag="PXD66_001378"
     CDS             4196..5650
                     /gene="gnd"
                     /locus_tag="PXD66_001378"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005714317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HDL6319675.1"
                     /translation="MSQKGDIGVIGLAVMGQNLILNMNDNGFKVVAYNRTTSKVDEFL
                     EGAAKGTNIIGAYSLEDLAAKLEKPRKVMLMVRAGEVVDQFIEALLPHLEEGDIIIDG
                     GNSNYPDTNRRVKALAEKGIRFIGSGVSGGEEGARHGPSIMPGGDESAWQYVKPIFQA
                     ISAKTDKGEPCCDWVGRDGAGHFVKMVHNGIEYGDMQLICEAYQFLKDGLGLSYDEMH
                     EIFKEWKNTELDSYLVDITTDILAYKDEDGEPLVEKILDTAGQKGTGKWTGINALDFG
                     IPLTLITESVFARCVSAFKDQRVAASRLFNKEIGKVEGDKKVWVEAVRRALLASKIIS
                     YAQGFMLIREASEQFGWDINYGNTALLWREGCIIRSRFLGNIRDAYEANPYLVFLGSD
                     PYFKDILENCLADWRKVVAKSVEIGLPVPCMASAITFLDGYTSARLPANLLQAQRDYF
                     GAHTYERTDKARGEFFHTNWTGRGGNTASTTYDV"
     gene            5785..6378
                     /locus_tag="PXD66_001379"
     CDS             5785..6378
                     /locus_tag="PXD66_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HDL6319676.1"
                     /translation="MLTDQDIFNRYQTKKSEFEKQADISLIGHSLFDMWGDMENGTPN
                     LAGKTVANLGLSGTSTRQYLDVITRQERIQHVGDNVFIFLGVNDIVKEPDYSPKQVLE
                     WIGEIVDSLRKINPNANFFLLEATPVNNITTTNNAAIKELNAYFEANIPSDLTYVKTW
                     EYFADKEGNLDLTLCHDGLHFTQAGYDRLEAILTKHL"
     gene            complement(6449..9313)
                     /locus_tag="PXD66_001380"
     CDS             complement(6449..9313)
                     /locus_tag="PXD66_001380"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012771641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDL6319677.1"
                     /translation="MTKQFTMEDRFNPSAVEQALYQHWESQGYFKPSEDINAPSYCIA
                     IPPPNVTGSLHMGHAFQQTLMDTLIRFNRMEGNNTLWQTGTDHAGIATQMVVERKIAA
                     EEGKTRHDYGREAFINKIWDWKAYSGGTISQQMRRLGNSIDWDRERFTMDEGLSNAVK
                     EVFVRLHEEGLIYRGKRLVNWDPKLHTAISDLEVENKESKGSLWHFRYPLANGAKTAD
                     GLDYLVVATTRPETMLGDTAVAVHPEDERYQALIGKSVILPLANREIPIIADEYVDRE
                     FGTGVVKITPAHDFNDYEVGKRHGLPMVNVMTLNADIRAEAEIIGTDGKPLSDYTAPI
                     PADYQGLERFAARKKIVADFEALGLLDQIKPHDLKVPYGDRGGVPIEPMLTDQWYVSV
                     KPLAEVATKAVEDGEIQFVPKQYENLYFSWMRDIQDWCISRQLWWGHRIPAWYDEQGN
                     VYVARDEAEVRAKHNLPADLPLKQDEDVLDTWFSSGLWTFSTLGWPEQTKELKMFHPT
                     DVLITGFDIIFFWVARMIMFTMHFIKDENGKPQVPFKTVYVTGLIRDEQGQKMSKSKG
                     NVLDPIDMIDGISLDDLLEKRTGNMMQPQLAEKIAKATRKEFAEGIVAHGTDALRFTL
                     TALASNGRDINWDMKRLEGYRNFCNKLWNASRYVLTNDKLDLSEGDVEFSLADRWIES
                     QFNRTVETFRTALSQYRFDLVANAIYEFTWDQFCDWYLELTKPIFFKGTDVQRRGASR
                     TLVNVLEKLLRLIHPVMPFITEEIWQKVKGFVGIEADTIMLQKFPQFDPLAIDETAES
                     QINFIKEVIVAVRNIRAESNIAPSKGLDLIARNFSADEVSILNANEVLLKSMAKLDSV
                     KVLENGENAPLSVAKLVANGEILIPMAGFINKEAELARLTKEMDKLKGEVARIEGKLS
                     NEAFVAKAPEQVIAKEREKMQEYLSGLEKLQVQYQEIEAL"
BASE COUNT         2663 a         1955 c         2080 g         2639 t
ORIGIN      
        1 taacaattag caataggata aataaatgaa tgctgaaaat agctgtatcg tcatttttgg
       61 tgcttccggt gacttaactt ttcgtaaatt aattcctgct ctttataact tatataagat
      121 tggcagattg ggcgagcatt tttccgtcct aggtgtttct cgttcagaat taaccgacga
      181 atctttccgc caaaaaatgc gtgatgcgtt agtcaaattt gaaaaagcaa gcggtgaaga
      241 attagataaa ttttgcgaac atctttatta ccaagctgta aatacctctg atgcagtgga
      301 ttatgccaag ttgctgcctc gtcttgatga gctacacgat aaatatcaaa cagccggtaa
      361 cacgctttat tacctctcca cgccaccaag tttatatggc gtgattccgg aatgtttagc
      421 cgctcacggc ttaactaccg aagagtttgg ctggaaacgg attattgttg aaaaaccatt
      481 cggctacgat atcgaaaccg ccaaaaaatt agacgtacaa atccacaaat gctttgaaga
      541 gcatcaaatc taccgtatcg accactattt aggtaaagaa acggtacaaa acttactcgt
      601 tttacgtttt tctaacggct tatttgaacc gctttggaac cgtaatttta ttgattatgt
      661 ggaaattacc ggggcagaat ccatcggcgt tgaagatcgt ggcggttact atgatggctc
      721 gggggcaatg cgtgatatgt tccaaaacca cttattgcaa gtattagcca tggttgcaat
      781 ggagccaccg gcaattatta atgctaattc aatgcgtgat gaagtcgcaa aagtgctata
      841 ttgcttgcat ccgttaactg aagaagacgt taaacacaat gtggtgttag gtcagtatgc
      901 tcgtggcacg gtagacggca aagaagtgcc ggcttacttg gaagaaaaag gcgtgccggc
      961 tgattccaac actgaaacct ttatggcggt aaaatgtaaa atcgataact ggcgttgggc
     1021 aggtgtgccg ttctatgtgc gaaccggtaa acgcttacca acccgtgtga cggaaatcgt
     1081 aattcatttt aaaactacac cacacccggt atttagccaa aatgctccgg aaaataaatt
     1141 gattattcgt gtgcaaccgg acgaaggcat ttcaatgcgt ttcggcttga aaaaaccggg
     1201 agcaggcttt gaagcgaaag aagtctcaat ggatttccgc tattctgatt taagttcatc
     1261 atcaagctta ctcaccgctt atgaacgctt actattagac gcacttaaag gtgatgcaac
     1321 cttatttgcc cgtactgatg ctgtacacgc ttgttggaaa tttgtgcaac cgattttagc
     1381 ctacaaagct aatcgtgggc gtgtgtatga atatgaatcc ggcacttggg ggccaaccga
     1441 ggcagataaa ttaatcgcca gacacggcaa agtatggcga aaaccgtccg gtacaatgaa
     1501 gaagaaagtg taaatttcag ttcccctctt tagcaaagag gggctagggg agatttggca
     1561 gaagttatat acgctcatac gaaatattga aatcgtaatc aaatctcccc ctcccctctt
     1621 ttctaaagag gggtgatcta acgaggcaaa ttatgaataa agttattttc cccaccgccc
     1681 aagctgcggt tgaacaaatt gctaaagagt ttattgaata cagccatcaa aaccgcccgg
     1741 tgcatatttc cctttccggt ggttcaaccc ccaaattatt attcaaaacc ttagctcaag
     1801 caccgtttaa taccgaaatt caatggcaaa acctgcattt ctggtggggg gatgatcgaa
     1861 tggttgcccc aaccgatccc gaaagtaact acggcgaagt gcagaaatta ctgttcgatc
     1921 acatcgacat tccaagcgaa aatattcacc gcattcgtgg tgaagagaat gtagaacaag
     1981 aacttgcaag attttcggca gaattgaccg cttgtgtgcc gaacttggcg tttgattgga
     2041 ttattttagg aatgggaaca gacgggcata ccgcctcact gttcccacat caaacagatt
     2101 ttaatgatca gaacgttgcc gtgatcgcca aatacccaga aacagggcag atccgcattt
     2161 ccaaaacggc atctttaatt gaaaaagcca aacggatcac ttacttggtc acaggagcaa
     2221 gcaaagcgga aattttaaaa gaaatccaaa ccactccggc agaaaattta ccttatcctg
     2281 ccgccaaaat tcaggcggaa aatggggtaa cggaatggta tttggatagt gaggcggcta
     2341 acttactcta ggtcacaatc agtgaccttt acaagcggtt aaattttaga aaaatgttgc
     2401 aaagtatttt tgtgatattc atcgaaaaag cagaaaaatc tgctcgtggt ataggccaaa
     2461 aattggcata gtctttctta ttttgagatc acaaaagtga tcttcactga agaataagga
     2521 agaaagatga atccaattca attaattgaa ccaaacatta actctaaaat ttttactatt
     2581 cgtggcgagc aagtaatgct agatgttcat ttggcagagc tttatcaggt agatactcga
     2641 acctttaacc aagcagtaaa acggaatgaa gagcgatttc ctgacatatt tcgttttcaa
     2701 ttaacagaaa acgaatggct atctttaaga tcacaatttg tgatcttaaa cgaaaatagt
     2761 gggcgtggcc aacataggaa atatcttcct tatgtcttta ccgagcaagg cattgcaatg
     2821 ctttcggcag tattgcgtag tgaagttgcg gtgcaagtta gcattaagat tatgcaagcc
     2881 tttgtgcaaa tgcgtcatct tttgcaaaca aatagtggtt tggtacaacg cttagaaagt
     2941 gtagagcgta aacagttgga gatgtctggg cagttagagc aggtgtttca atctcttgaa
     3001 ggcaataagc ctaaaatgca aggggttttc tttgaaggtg aaacctttaa cgcttatcag
     3061 tttgttacgg aattagtgag aggtgcaaaa tcatcgctga ttttaattga taactatgta
     3121 gatgataccg ttttaacttt atttacgaaa cgatcgcata acgtatcagt tacgatttat
     3181 acgaaacaaa tatctaagca acttgcatta gatttggaaa aatataacag ccaatatgct
     3241 cacattaatg tgaaaatatt tgataaatct cacgagcgat ttttgattat tgatgaaaaa
     3301 gagctttatc atatcggtgc atcacttaaa gatttaggta agaaatggtt cgctttttct
     3361 aaaatggatt tatcaatgag ccgcatttta gaaaagttag cggaaaatca ataaggtata
     3421 ttaatgaaaa cactcggcat tttaggtgga atgagtgcgg aaagcacggt ttcttattat
     3481 ttagatatta atcgctcggt gaataaagta ttaggtggaa atagcagtgc caagatttta
     3541 atttcgagtg tcaattttga agagattgta caatgccaaa agcagggaaa ttggcaagca
     3601 gccgctgaaa ttttatctga acaagctaga ttgctagaac aagccggagc agaaggcatt
     3661 ttattggcaa ccaacacaat gcacaaaatt gccgatcaaa ttagtagcca tatctctgtg
     3721 ccgtttttgc atattttgga ctgtgtggct gaggcgattt cagctcaagg tctgcagaaa
     3781 gtagcactcc ttggcacaaa atttactatg agtgataatt tttacaaaga cggtttattg
     3841 gagcgtggta tcacatcaat agtaccagat gaggaaacgc aacaagaaat tcatcgcatt
     3901 attttcgagg aactctgttt aggcaagatt actgaaagtg ccaaagcgtt ttatcttcag
     3961 actattcaac aacttagtga acaaggtgca gaaggtgtga ttttaggttg taccgaaatc
     4021 gggttgctga ttaaccaaaa cgacaatccg ttaccgtttt tcgatacagc agcattacac
     4081 agccaaatgg cggtggagtt tattttaggt taataaatgt tacaagcggt caaatttgca
     4141 aaaaatttta ccaattcgac cgcttgcata gaatcaaaaa caataggagc aaacaatgtc
     4201 acaaaaaggc gatatcggcg tgattggact cgccgtaatg ggtcaaaatt taattttgaa
     4261 tatgaatgac aacggcttta aagtggtggc gtacaaccgc actacttcaa aagtggatga
     4321 gttcttagaa ggtgcggcga aaggcacgaa cattatcggt gcttactcgt tagaagattt
     4381 agcggcgaag ttggaaaaac cgcgtaaagt gatgctaatg gtgcgtgccg gtgaggtggt
     4441 ggatcagttt atcgaggctc ttctgccaca tttagaagaa ggcgacatca tcattgatgg
     4501 cggtaactca aactatccgg acaccaaccg ccgtgtgaaa gcgttagcgg aaaaaggcat
     4561 tcgctttatc ggctcaggtg tatcgggcgg cgaagagggt gctcgtcacg ggccgtcaat
     4621 tatgccgggc ggtgatgaat ctgcgtggca atatgtgaag ccgattttcc aagcaatttc
     4681 agccaaaacc gacaaaggtg agccttgttg tgactgggtt ggtcgtgacg gagcagggca
     4741 ttttgtgaaa atggttcaca acggtatcga atatggcgat atgcagttaa tttgtgaagc
     4801 ctaccaattc ttaaaagatg gtttaggttt aagctatgat gaaatgcacg aaatctttaa
     4861 agagtggaaa aacaccgaat tagacagcta tttggtggat attaccactg atattcttgc
     4921 ctacaaagac gaagacggcg agccattggt agagaaaatt ctcgacaccg caggtcaaaa
     4981 aggaacgggt aaatggactg gtattaacgc ccttgatttc ggcattccat taacactgat
     5041 taccgagtcg gtattcgccc gttgcgtatc tgcgtttaaa gatcaacgtg ttgcggcttc
     5101 aagattgttt aacaaagaaa ttggtaaagt tgaaggcgac aaaaaagtat gggtagaagc
     5161 ggtacgccgt gcgttacttg cttccaaaat catctcttat gcacaaggct ttatgttgat
     5221 tcgtgaggct tctgagcaat tcggttggga catcaactac ggcaataccg ccctattatg
     5281 gcgtgaaggt tgtattatcc gcagccgttt cttaggcaac attcgtgatg cgtatgaagc
     5341 caacccatat ttagtgttct taggctcaga cccatacttc aaagatattt tggaaaactg
     5401 cttggcagat tggcgtaaag tagtggcaaa atcggtggaa atcggtttac ctgtaccttg
     5461 tatggcgtca gccattacct tcttagatgg ctacacctcg gcacgtttac cggcaaactt
     5521 actccaagca caacgtgact actttggtgc tcatacttat gagcgtacag acaaagctcg
     5581 cggtgagttc ttccacacca actggacagg acgtggcggt aataccgcat cgaccactta
     5641 tgatgtgtaa ttaatctttt tgagccactt aattcgatat taagtggctt ttttattgga
     5701 tttttcttca aaatatcttg gagtgaatgt ttacaagttt gtataataat ggagcgattt
     5761 ttcatttata tttaaggaga acctatgctt accgatcaag atatttttaa tcgctaccaa
     5821 acaaaaaaat cagaatttga aaaacaggca gatatttccc ttattgggca ttccctgttt
     5881 gatatgtggg gcgatatgga aaacggcacg ccgaatttag caggaaaaac agttgccaat
     5941 ttgggcttat caggcaccag cacccgccaa tatttggatg tgattacccg acaagagcga
     6001 attcaacacg ttggcgataa tgtatttatt tttctaggcg ttaatgacat tgtaaaagag
     6061 ccggattact cgccaaaaca agtgttagag tggattggcg aaattgtgga ttctcttcgc
     6121 aaaattaatc caaatgccaa cttttttcta ttggaagcca caccggttaa taatattacc
     6181 accaccaata atgctgcaat aaaagagctc aatgcgtatt ttgaggcaaa tattccatct
     6241 gatttgactt atgtaaaaac gtgggaatat tttgctgaca aagaagggaa tttagatcta
     6301 actttatgcc acgatggatt acacttcacc caagcaggct atgaccgcct agaggcgatt
     6361 ttaaccaaac atttataatg aaaagcttca ataagcaagc ggtctgaatt agcaaaaaat
     6421 ttccaaatcc tgaccgcttg ttagttaatt acagtgcttc aatctcttga tactgcacct
     6481 gcaatttctc taatccactc aggtactctt gcattttttc acgctctttg gcgatgactt
     6541 gctctggggc tttggcgaca aaggcttcgt ttgaaagttt gccttcaatg cgagccactt
     6601 cacctttaag tttatccatc tctttggtga ggcgagctag ctcagcttct ttgttgataa
     6661 agcctgccat tgggatcaag atctcaccat ttgcgaccag tttcgctacc gatagcggtg
     6721 cattttcacc attttccagc actttcacgc tatcaagttt tgccatcgat ttgagcaaca
     6781 cttcgttagc gttgagaata gagacttcgt ccgcagagaa attacgagcg attaaatcta
     6841 aacctttgct tggtgcgatg ttgctttccg cacgaatgtt acgcaccgcc acaatcactt
     6901 ctttgataaa gttgatttga ctttcggcgg tttcatcaat ggcaagcggg tcaaattgtg
     6961 ggaatttttg caacataatg gtatcggctt caatacctac aaagcctttc actttctgcc
     7021 agatttcttc ggtgatgaac ggcattactg ggtggattaa acgcaggagt ttttccaata
     7081 cattcaccag cgtgcgactt gcgccacggc gttgtacatc tgtgcctttg aagaaaatag
     7141 gtttggtcag ctctaaatac cagtcgcaga attgatccca agtaaattcg tagatcgcat
     7201 ttgccactaa gtcgaaacgg tattggctga gtgcggtgcg gaaagtttcc accgtgcggt
     7261 tgaattggct ttcaatccaa cgatcggcaa ggctaaattc cacgtcgcct tcgcttaaat
     7321 ccaacttgtc attggtcagc acataacggc tcgcattcca cagtttgttg cagaagttgc
     7381 ggtagccttc taaacgtttc atatcccagt tgatgtctcg accgttgctg gcaagggcgg
     7441 tgagggtgaa acgcagagcg tctgtgccgt gtgccacaat cccttcggca aattctttgc
     7501 gggttgcctt agcgattttt tctgccagtt gtggctgcat catattacct gtacgttttt
     7561 cgagcaaatc gtcgagactg atgccgtcaa tcatatcaat cgggtctaac acgttccctt
     7621 tcgatttcga cattttctgc ccttgctcgt cacggatcaa gcctgttaca tacacggttt
     7681 tgaacggcac ttgcggtttg ccgttttcat ctttgatgaa gtgcatcgtg aacataatca
     7741 tacgagccac ccagaagaag atgatgtcaa aaccggtaat cagcacatcg gtcgggtgga
     7801 acattttcag ttctttggtt tgttctggcc agcctaacgt tgagaacgtc catagaccag
     7861 acgagaacca agtatccagt acgtcttcat cttgctttaa tggtaagtcc gcaggtaagt
     7921 tgtgtttcgc acgcacttcc gcttcatctc gtgctacata aacattgccc tgctcgtcat
     7981 accacgctgg aatgcggtgt ccccaccata attggcgaga gatacaccag tcttgaatat
     8041 cacgcatcca agagaaatac aggttttcgt actgtttcgg cacgaattgg atttcgccgt
     8101 cttccaccgc tttggtcgcg acttccgcca aaggtttgac agacacatac cattggtcgg
     8161 tcagcatcgg ctcaatcggc acgccaccac ggtcgccata cggcactttt aagtcgtgtg
     8221 gtttgatttg atctaacagc ccaagagctt caaaatctgc cacaattttc ttacgcgcgg
     8281 caaaacgctc taagccttga tagtcggcag gaattggggc ggtgtagtcg gaaagcggtt
     8341 taccgtctgt gccgataatt tcggcctctg cacgaatatc ggcatttaag gtcatcacat
     8401 tcaccatcgg caagccgtgg cgtttgccca cttcgtagtc gttgaaatcg tgggcagggg
     8461 tgatttttac cacgcccgta ccgaactcac gatccacata ctcatcggca ataatcggga
     8521 tttcacggtt ggcaagcggt aggattacgc ttttgccaat cagagcttga taacgctcat
     8581 cttcagggtg aaccgccacc gcagtatcgc ccaacatcgt ttcaggacgg gtcgttgcca
     8641 ccactaagta atccaagccg tctgcggttt ttgcaccatt agccagcgga tagcggaaat
     8701 gccacagcga gcctttgctt tctttgtttt ccacttccaa gtcagaaatc gcagtatgca
     8761 gtttcggatc ccagtttacg aggcgtttgc cacggtaaat caagccttct tcgtgcaagc
     8821 gaacgaacac ctcttttacc gcattggata agccttcgtc catcgtaaaa cgctcacgat
     8881 cccagtcaat cgagttgcct aagcgacgca tttgctggct gatcgtgccg cccgaatagg
     8941 ctttccaatc ccagattttg ttgataaagg cttcacgccc ataatcgtgg cgggttttac
     9001 cttcttccgc tgcgatttta cgctccacca ccatctgagt ggcaatccct gcgtggtcag
     9061 ttcctgtttg ccaaagggta ttgttgccct ccatacggtt aaaacgaatc agcgtatcca
     9121 tcaaggtttg ctggaaagcg tgccccatat gcaaactccc cgtcacattc ggcggcggaa
     9181 tagcgataca gtagctcggt gcattaatat cttcactcgg cttaaaatag ccttgcgatt
     9241 cccagtgttg gtagagcgct tgctctacag cggaagggtt aaaacggtct tccattgtga
     9301 attgttttgt cattggtttt gttttctctt gtttaat
//
LOCUS       DANLXF010000090         9023 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.090, whole genome shotgun sequence.
ACCESSION   DANLXF010000090 DANLXF010000000
VERSION     DANLXF010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9023
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.090"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            123..839
                     /locus_tag="PXD66_001381"
     CDS             123..839
                     /locus_tag="PXD66_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL6319678.1"
                     /translation="MKKTLLTLLFGCVVTAQAQDIKFVMEPSYPPFEMTEEKGEIIGF
                     DVDIANAICKEMNANCTFHSQPFDSLIQSLKQKQFDAAISGMGITEPRKKQVLFSEPY
                     FPSSAAFIAKKDTDFAKVKTIGVQNGTTYQHYLAKEKKEYNVKSYASYQNAILDVQNG
                     RIDAIFGDVPVLAEMARKHEGLDFVGEKINNPNYFGDGLGIATHLKNQVLVDQFNAAL
                     KTIKENGEYQKIYDKWMGGK"
     gene            complement(909..1787)
                     /locus_tag="PXD66_001382"
     CDS             complement(909..1787)
                     /locus_tag="PXD66_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HDL6319679.1"
                     /translation="MKNLYKWPAAQSLYPNQGKKSYAGKRFRYRLRSWLHHSKIKQFE
                     QFVTQNPQLIPLLNARPNYSYPVAHRFLDKRFSAKQRLQKITDNLLFLPQKLAHLPPL
                     WEQAVNFGEIIADFELWLNINEHQPMEGFWALELRHRPTNQLVYLLTFGKLDEALLIA
                     VIQGPNFEGSKELVKQLTKSCHGLRPAYLMVETMKALTAVLGYQSLLGIPQKYQNKSR
                     WVQSKRYVVDYDAIFAESGGKPGNYWQLPLELESKKIDEIPSKKRSMYRKRYEMLANL
                     KQTMELMLKTYTGLNG"
     gene            complement(1863..3188)
                     /gene="hslU"
                     /locus_tag="PXD66_001383"
     CDS             complement(1863..3188)
                     /gene="hslU"
                     /locus_tag="PXD66_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HDL6319680.1"
                     /translation="MMSMTPREIVSELDAHIIGQKDAKRAVAIALRNRWRRMQLPEDL
                     RQEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LADVSMKLVRQQAVEKNKMRAQDAAEDRILDVLLPPAKDQWGNVQESDNNSTRQTFRK
                     KLREGQLDDKEIEIDVAAQVSVEIMTPPGMEEMTSQLQSLFEGMSPSKTKKRKMKIKD
                     ALKVMLDEEAAKLVNPEELKQQAIEAVEQHGIVFIDEIDKICKKGEHSGGDVSREGVQ
                     RDLLPIIEGSTVNTKHGMVKTDHILFICSGAFQVARPSDLLPELQGRLPIRVELKSLT
                     KEDFERILTEPNASLTLQYRELMKTEGVEIEFTKDGISRIAESAFRVNEKTENIGARR
                     LHTVLERLMDGISFDASERRGEKIIIDENYVSEALNDVVENEDLSRFIL"
     gene            complement(3445..3966)
                     /gene="hslV"
                     /locus_tag="PXD66_001384"
     CDS             complement(3445..3966)
                     /gene="hslV"
                     /locus_tag="PXD66_001384"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HDL6319681.1"
                     /translation="MTTIVCVRKDGKVAIGGDGQATLGNCIEKGTVRKVRRLYKDKVV
                     TGFAGSTADAFILRDLFEKKLELHQGHLVKSAVELAKEWRTERALRRLEAMMIVANDS
                     EFLLVSGSGDVIEPEQDVLAIGSGGNYAKAAALALLRTENNLSAKEIVAEALKIAGDI
                     DIYSNYNHVIEEV"
     gene            complement(4027..4740)
                     /gene="sfsA"
                     /locus_tag="PXD66_001385"
     CDS             complement(4027..4740)
                     /gene="sfsA"
                     /locus_tag="PXD66_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDL6319682.1"
                     /translation="MKFPPLSHGILLRRYKRFLADIQLPNGEQITLHCPNTGAMTGCA
                     NPGDTVWFSTSHNPKRKYAHTWELTQTQAGDFICVNTLRANQLVQEALENQWISELAD
                     YQHILPEQKYGSENSRIDFLLKSDLLPDCFVEVKSTTLLTENGVGMFPDAKTERGQKH
                     LRELTKIAEQGLQAVIFFAILHTGIERFEVAKQIDPKYAELFAQAIAQKVQVLSYKMA
                     ISLENGQPIAATLTKKCEF"
     gene            complement(4756..5625)
                     /locus_tag="PXD66_001386"
     CDS             complement(4756..5625)
                     /locus_tag="PXD66_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDL6319683.1"
                     /translation="MLYLLIATLIWASSFIVGKVAISAFDPVQIMQIRLTLAAFIALP
                     FFIPAYKKIQKNQHIKVWLISFLTFPLYVILQFSGLKLTSAASAVALLGLNPLLTVLV
                     GYFCFKKQATRLDFILSLFACLGILIMASGSDDSGSINVLGLLLVTLGSLSFIVGMYL
                     SKEILQSVKVIDLTKIMLVQGAITALPITLLFAENWQLPTSLSAWASVAYLGIICTSL
                     AILLWNKGISQSSPILAGILLALEPVFGLLMALVFLSEQLSLVTWVGIIIVICTALLS
                     VLLPMMKRYKRLI"
     gene            complement(5626..6018)
                     /locus_tag="PXD66_001387"
     CDS             complement(5626..6018)
                     /locus_tag="PXD66_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL6319684.1"
                     /translation="MKSPILGFHHIALIVSDYEKSKHFYTRILGAEIIAETYREQRAS
                     YKLDLRFKDGSQIELFSFPETPPRFTSPETCGLRHLAFRVENIEYAIAFLEQHQLPHE
                     GIRIDEITGKRFTFFRDPDDLPLEFYEV"
     gene            6102..7247
                     /gene="mutY"
                     /locus_tag="PXD66_001388"
     CDS             6102..7247
                     /gene="mutY"
                     /locus_tag="PXD66_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDL6319685.1"
                     /translation="MNSNSPFPLAKAKASDPFAKAVLAWFEQYGRKHLPWQQNKTLYQ
                     VWLSEVMLQQTQVATVIPYFARFMERFPTVIDLANASIDEVLHLWTGLGYYARARNLH
                     KAAIQIRDEFGGEFPTRFDDVLALTGVGRSTAGAILSSVLDAPHPILDGNVKRVLSRY
                     FAVEGWAGEKAVENRLWQLSESVTPDTQVADFNQAMMDLGAMICTRTKPKCLLCPLQE
                     NCKTNAMQAWDSFPAKKPKKTLPERQTYFLVLKSGSKVLLQKREAKGIWGGLFVFPQF
                     ESLEALKRSDFMQNLQISQQLTAFRHTFSHFHLDITPVLVETDLKKTSENSPLVVEEK
                     AGNYLSKVSSNADYWYDLHQHNEVGLATPVKRILDELAFSVKNEIKD"
     gene            7251..7520
                     /locus_tag="PXD66_001389"
     CDS             7251..7520
                     /locus_tag="PXD66_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HDL6319686.1"
                     /translation="MARTVFCEYLKQEAEGLDFQLYPGELGKRIFDSISKQAWSEWIK
                     KQTMIVNEKKLSMMNPEHRKLLEQEMVNFLFEGKEIIIEGYKPVE"
     gene            7537..8628
                     /gene="mltC"
                     /locus_tag="PXD66_001390"
     CDS             7537..8628
                     /gene="mltC"
                     /locus_tag="PXD66_001390"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HDL6319687.1"
                     /translation="MKKYAKYLPMLAIIPFLIACGSNKSTKKPKSTRVDYSKDTNGLD
                     ILAGQFSHNIDDIWGSNELLVASKKDYVKYTDKFYTRSHISFEEGVITIETLGNQNHL
                     RNSIIHTLLMGRDPKGIDLFASGDAPISSNPFLNGQVEDQFGRNITNVAIANDFATYL
                     LQNKVQTRRLKNGQTVSFVTIKMVAGHIEVRARQYLPLVRKMAKQYGIEPSLILGIME
                     VESAFNPYAISYANAIGLMQVVPRTAGRDIFARKGMGGQPSREYLYNPSNNIDAGTLY
                     LTILRDEYLDGIEDPVAKRYAMISAYNSGAGAVLRVFDSDRYSAIDRINDLSSDAVYR
                     ILTTAHPSSQARNYLRKVTTAREKYRNIR"
     gene            8760..8835
                     /locus_tag="PXD66_001391"
     tRNA            8760..8835
                     /locus_tag="PXD66_001391"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8793..8795,aa:Phe,seq:gaa)
     gene            8841..8916
                     /locus_tag="PXD66_001392"
     tRNA            8841..8916
                     /locus_tag="PXD66_001392"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8874..8876,aa:Asn,seq:gtt)
BASE COUNT         2617 a         1829 c         1910 g         2667 t
ORIGIN      
        1 aactacatct caatttgatg tgtttgtatg acataaaaca aactttatga aaagaagact
       61 ttttttaaga aagtaactgc gttaaagtgg taatccttat aatgaaaaca cagggacttt
      121 ttatgaaaaa aacacttttg acccttttat tcgggtgtgt ggttactgcg caggcacaag
      181 atatcaaatt tgtgatggag ccaagctatc ctccatttga aatgaccgaa gagaagggag
      241 aaattatcgg ttttgatgtg gatattgcaa atgctatctg taaagagatg aatgcaaatt
      301 gtacattcca tagccaacct tttgatagct taatccaaag cctaaaacaa aaacaatttg
      361 atgctgcaat ttcaggtatg ggtattacgg aaccacgtaa gaaacaagta ttatttagcg
      421 agccttactt tccaagctct gctgccttca ttgcgaaaaa agatacagat tttgctaagg
      481 tgaaaacgat tggtgtgcaa aatgggacta cttaccaaca ctatttagca aaagagaaaa
      541 aagagtacaa tgtgaaatct tatgcctctt atcaaaatgc tattttagat gtgcaaaatg
      601 ggcgaattga tgctattttt ggcgatgtgc cggtattggc tgagatggca agaaaacacg
      661 aagggctaga ttttgtaggg gagaaaatca ataatccaaa ctattttggt gatggtttag
      721 gcattgcaac tcacttgaaa aaccaagtat tagttgatca attcaatgca gcattaaaaa
      781 cgattaaaga gaatggcgaa tatcaaaaaa tctatgataa atggatgggc ggcaagtaac
      841 ttgtaagcgg tcattttttg caaagttttg caaagttttg cagattttga ccgcttgtat
      901 tctttgattt acccatttaa gccagtataa gttttaagca taagctccat ggtctgcttt
      961 aaattagcta gcatttcata acgcttgcga tacattgagc gttttttgct tggaatttca
     1021 tctatttttt tactttccaa ttcaaggggt aactgccaat aatttcccgg ctttccgccc
     1081 gattccgcaa aaatagcatc ataatccact acatagcgct tactttgcac ccagcgggat
     1141 ttattctggt atttctgtgg aatgcctagt aaactctgat agccaagaac agccgttaaa
     1201 gccttcatcg tttcaaccat taaataggcc gggcgtaagc cgtggcaact tttggttaat
     1261 tgtttcacca gctcttttga accttcaaaa ttcggacctt gaattacggc tatcaacaag
     1321 gcttcatcta acttaccaaa tgttaataaa taaactagct gattagtcgg tctgtggcga
     1381 agttctaatg cccaaaaacc ttccatcggc tgatgttcat tgatgttaag ccataactca
     1441 aaatctgcaa taatttcacc aaaattgacc gcttgttccc atagaggtgg taaatgagcc
     1501 aatttttgcg gtaaaaacag caggttatct gtgatttttt gtaagcgttg ttttgcactg
     1561 aaacgcttat ctaaaaagcg gtgagccacg gggtagctgt aatttggtcg agcatttaag
     1621 agcggaatca gttgtggatt ttgtgtaacg aactgctcaa attgcttgat tttggaatga
     1681 tgcaaccaag agcgaagacg gtaacgaaaa cgtttccctg cgtaagactt ctttccctga
     1741 tttggataaa gcgattgtgc agcaggccat ttgtatagat ttttcatttt tacaactaac
     1801 aatatgaaaa gcggctaatt cgcaagattt ttcgctaaaa taaccgcttg tataacttaa
     1861 aactacaaaa taaagcgact taaatcttcg ttttccacca cgtcatttaa cgcttcggaa
     1921 acatagtttt catcaatgat gattttttca ccacggcgtt cgctggcatc gaacgaaatg
     1981 ccgtccatca aacgctcaag cacggtgtgt aaacgtcttg ccccgatgtt ttcggttttc
     2041 tcgttcacac ggaatgcact ttctgcaata cggctgatac cgtctttagt gaactcaatc
     2101 tccacgcctt cagttttcat caactcacga tattgcaagg ttaatgaggc attcggttcg
     2161 gttaaaatac gctcaaaatc ttctttggta agcgatttta actccacacg aatcggcaag
     2221 cgaccctgta attctgggag taaatcagac gggcgagcca cttggaatgc ccccgagcag
     2281 ataaacagaa tatggtcggt tttcaccata ccatgcttgg tatttaccgt tgagccttca
     2341 atgattggaa gtaaatcacg ctgaacacct tcacgagaaa catctccacc cgaatgctcg
     2401 ccttttttac agattttgtc gatctcatca ataaagacaa taccgtgctg ttccaccgct
     2461 tcaatcgctt gctgtttcag ctcttcagga tttactaatt tcgccgcttc ttcatcaagc
     2521 atcaccttta acgcatcttt gattttcatt ttgcgttttt tggttttgct tggcgacatt
     2581 ccctcaaaca gcgattgtaa ttgcgaggtc atctcttcca ttcccggcgg ggtcataatc
     2641 tcaacgctca cttgagctgc cacgtcaatt tcaatctctt tatcgtctaa ttgcccttca
     2701 cgcaattttt tgcggaaagt ttggcgggta gaattattgt cggattcctg cacatttccc
     2761 cactgatctt tcgccggtgg caataatacg tctaaaatgc gatcttccgc tgcatcttgt
     2821 gcacgcattt tatttttttc gaccgcttgt tggcggacta atttcatcga cacatcagct
     2881 agatcacgga taatcgaatc cacttcttta cccacatacc ccacttcagt gaatttggtt
     2941 gcctcaactt tgataaaagg ggcattggca agcttcgcca aacggcgggc gatttcggtt
     3001 ttacccacac cggtcggacc gatcattaaa atatttttgg gggtcacttc ttggcggaga
     3061 tcttccggca actgcattct tctccagcgg ttacgcaaag caattgctac cgcacgtttt
     3121 gcatcttttt gtccgataat gtgtgcgtct aattctgaga cgatttcacg gggtgtcatt
     3181 gacataatgt tattccttaa attaattacc ctctcccctt gtgggagagg gacagatttt
     3241 tctttgttca gaagaaaaat cagggagagg gtaacaagcg gtcttttttc ttaaacgttt
     3301 tgcattaacc cctctctctg aaaaatattt ctgaacgaaa tatttttcgg tctcaatact
     3361 cgctacgctc gctcctgtgg agccgttggc aaagccaacg ttcaaatgta aacatttgtc
     3421 ccacaagggg agagagttat ttaactaaac ttcttcaatc acgtgattgt agttactgta
     3481 aatatcaata tcgccggcga tttttaacgc ttccgcaacg atttcttttg cagataaatt
     3541 attttcggtg cgtaataacg caagagcagc ggctttcgca tagttaccgc ctgaaccaat
     3601 cgccagcaca tcttgctccg gctcaatcac atcgcccgaa cctgaaacca ataaaaactc
     3661 gctgtcattg gcgacaatca tcatcgcttc taaacggcgt aaagcacgct cggtacgcca
     3721 ctcttttgcc agttccactg ccgatttcac taaatgccct tggtgtaact ccaatttttt
     3781 ctcgaataaa tcacgcaaaa taaaggcatc agcggtcgag ccggcaaagc ctgtcaccac
     3841 tttgtcttta tacaaacggc gaactttccg caccgtgcct ttctcgatac aatttccaag
     3901 cgttgcctga ccatcaccac cgatagccac ttttccgtct ttacgcacgc atacaatcgt
     3961 tgtcatacta aatccttctt tttaaaaact ttggcttata tggggatatg ctgtgtgttt
     4021 tcaagcttaa aactcacatt tctttgttaa ggttgccgca ataggttgcc cgttttccaa
     4081 tgaaattgcc attttatagg agagaacctg cactttttgt gcaatggctt gagcgaataa
     4141 ttcggcatat ttggggtcta tctgctttgc cacttcaaac cgttcaatac ctgtgtgcaa
     4201 aatagcgaaa aaaatgaccg cttgtaagcc ctgttcagcg attttcgtca gctcccgcaa
     4261 atgtttttgc ccccgctcgg ttttggcatc ggggaacatt cccacaccgt tttcggttaa
     4321 aagggtggta gatttcactt ccacaaaaca atccggcaga aggtccgatt tgagcaaaaa
     4381 atcaatccga ctgttttcac tgccatattt ttgttcaggc agaatgtgct gataatccgc
     4441 aagctcgcta atccattgat tttccaaggc ttcttgtaca agctgattgg ctctcaaggt
     4501 attgacacaa ataaaatccc ccgcttgggt ttgggttagt tcccaagtgt gagcatattt
     4561 acgtttgggg ttatgagagg tggaaaacca aactgtatca ccggggttgg cacagcctgt
     4621 catcgcaccg gtatttgggc aatggagggt aatttgttcg ccatttggca actgaatatc
     4681 ggcaagaaaa cgtttataac ggcgaagtaa aatgccgtgg gaaagagggg gaaatttcat
     4741 ttgcaaaaaa tcctgttaaa ttaaccgctt gtatcttttc atcatcggta gcaaaacgga
     4801 gagtaaggcg gtgcaaataa caataataat cccaacccaa gtcactaaac tgagctgttc
     4861 tgataaaaag accaatgcca ttagcagtcc gaataccggt tcaagtgcga gtagaatacc
     4921 tgcaagaata ggcgaagatt ggctgatgcc tttgttccat aacaatattg ccaagctggt
     4981 acaaataatg cctaaatatg ctacgctcgc ccatgctgag agactggttg gcagttgcca
     5041 attttctgca aagagtaagg taataggtaa ggcggtaatt gccccttgca ccagcatgat
     5101 cttggttaaa tcgatcactt ttactgattg cagaatctct ttgctgaggt acattcccac
     5161 aataaaactt aaactgccta aggttacaag caataatcct aatacattga ttgaaccgct
     5221 atcatcgcta cccgatgcca taattaaaat ccccaagcag gcaaataaac ttaggataaa
     5281 atcgagcctg gttgcctgtt ttttgaaaca aaaatagccg actaacacag ttaaaagcgg
     5341 gtttagcccg agtagtgcca cggcacttgc tgcggaagtt aatttcaagc cggaaaattg
     5401 taagatcacg tacagaggaa aagtgagaaa actaatcagc cagactttaa tgtgctgatt
     5461 tttctggatt tttttatacg caggaataaa aaacggcaga gcgataaacg ccgctaacgt
     5521 cagccgaatt tgcataattt ggacagggtc aaaggctgaa atcgccactt tacctacaat
     5581 gaatgaactc gcccaaatca gggttgcaat cagtaaatat aacatttaga cctcgtaaaa
     5641 ctctaacggc agatcgtccg gatctcggaa aaacgtgaag cgtttgccgg tgatctcatc
     5701 aattcggatc ccttcatgcg gtaattgatg ttgttctaaa aatgcgatgg catattcaat
     5761 attttccact cgaaatgcca aatggcgaag cccgcaggtt tcgggcgagg tgaaacgagg
     5821 cggcgtttca ggaaaactga ataattcaat ttgtgagcca tctttaaagc gtaaatctaa
     5881 tttatagctg gcacgttgct ctcggtaggt ttctgcgatg atctctgccc ctaaaatgcg
     5941 ggtgtagaaa tgtttggatt tttcgtaatc ggaaacaatt aatgcaatgt ggtggaaacc
     6001 taaaatgggc gatttcatct tttttcctta atgtgtaaaa tttttggtaa aactgaccgc
     6061 ttgcagtatg ataatttatt tttcggattt caagattttt aatgaacagc aattccccat
     6121 ttcctttagc gaaagcaaaa gcttctgatc cgtttgccaa agctgtttta gcttggtttg
     6181 agcaatatgg gcggaaacat ttaccttggc agcaaaataa aacgctgtat caggtttggt
     6241 tgtcggaggt aatgttgcaa caaactcaag tagcgaccgt tattccttat tttgcacgtt
     6301 ttatggaacg atttcccacc gtaattgact tagctaatgc ctcgattgat gaagtattgc
     6361 atttgtggac agggctgggt tattacgctc gggcaagaaa tttgcataaa gcggcaattc
     6421 aaattcgaga tgagtttggc ggtgaatttc ctacccgatt tgatgatgtg ttggcattaa
     6481 ccggagtggg cagaagtact gcaggggcga ttttgtcttc tgtgttagat gccccacacc
     6541 cgattttaga tggcaatgtc aaacgggtgt taagccgcta ttttgcagta gaaggctggg
     6601 cgggtgaaaa agcggtggaa aatcgccttt ggcaattaag cgaaagtgtg acgcctgata
     6661 cccaagtggc agactttaac caagcgatga tggatttagg ggcgatgatt tgcacccgta
     6721 caaagcctaa gtgcttgctc tgcccgctac aagaaaattg taaaacgaat gcaatgcaag
     6781 cgtgggatag ctttcctgcc aagaaaccga aaaaaacctt gccggagcgt caaacctatt
     6841 ttttagtgct gaaaagcggc tcgaaagtgc tgttacaaaa acgggaagca aaaggcattt
     6901 gggggggctt atttgtgttt ccgcaatttg agagcttaga ggctctcaag cggtctgatt
     6961 ttatgcaaaa tttgcaaatt tcacagcaat taactgcatt taggcatact tttagccatt
     7021 ttcatttgga tattaccccg gttttagtag aaaccgattt gaaaaaaaca agtgaaaatt
     7081 caccgcttgt tgtcgaagaa aaagcaggaa attatctttc taaggtatct tcaaatgcgg
     7141 attattggta tgatttgcac caacataacg aagtgggttt agcaacgccg gttaaacgta
     7201 ttttagatga gctggcattc agtgtgaaaa atgagattaa ggattaaatt atggctcgta
     7261 ccgtattttg cgaatattta aaacaagagg cagaaggttt agattttcaa ctttatccgg
     7321 gggagctggg taagcgtatt tttgactcaa tcagcaagca agcgtggtct gagtggatta
     7381 aaaaacagac aatgatcgtc aatgagaaaa aactcagtat gatgaacccc gaacaccgta
     7441 aactgctcga acaagagatg gtcaattttt tatttgaagg taaagagatc atcatcgaag
     7501 gctataagcc ggtcgaataa taaaggttaa atcacaatga aaaaatatgc taaatatttg
     7561 ccgatgttgg caattattcc gtttcttatt gcttgtggga gcaataaatc gactaagaag
     7621 ccaaaatcta cccgagtaga ttattctaaa gataccaatg gtttggatat tttggcaggg
     7681 cagttttctc acaatattga tgatatttgg ggaagtaacg agctgttagt tgccagtaag
     7741 aaagattatg tgaaatatac ggataagttt tacacccgta gccatatttc gtttgaagaa
     7801 ggggtgatta cgattgagac tttagggaat cagaatcatc tacgcaattc gattattcat
     7861 actttattaa tgggcagaga cccgaaaggg attgatttat ttgcctcagg cgatgcaccg
     7921 attagctcta accctttcct aaacggacag gttgaagatc aattcgggcg aaatattacc
     7981 aatgttgcca tcgccaatga ttttgcgact tatttgttgc aaaataaggt gcaaacacgc
     8041 cgtttgaaaa atggtcaaac tgtgagcttt gtaacgatta aaatggtggc aggacacatt
     8101 gaagtacgtg ccagacaata tttaccgctg gtgcgtaaaa tggcgaagca gtatggtatt
     8161 gagccaagtt tgattttagg gattatggaa gtcgaatcag cattcaaccc ttatgccatc
     8221 agctatgcca atgcgattgg cttaatgcag gttgtacctc gtaccgccgg gcgtgatatt
     8281 tttgcccgta aaggtatggg agggcagcca agtcgtgaat atctttataa tccatcaaat
     8341 aacattgatg caggtacgct ttatttgact attttgcgtg atgaatactt agatggaatt
     8401 gaagatcctg tggctaaacg ctatgcaatg atttctgcat acaatagcgg agcaggtgcg
     8461 gtattacgag tgtttgattc agaccgatac tctgcgattg atagaattaa cgatctttcg
     8521 tctgatgcgg tttatcgaat tttaaccact gctcatccat cttctcaagc tagaaattat
     8581 ttaagaaaag taaccaccgc cagagagaaa tatcgcaata ttcgttaatg tgtgaattat
     8641 gctgtttttt agccgaaaag tataaaaaaa gcacatttga gcagcggttt ttttaaattt
     8701 gcaaaaaaac gtttgacgtt gcaagctgaa atcagcataa tgcaccacgc aacgacgttg
     8761 cctcgatagc tcagtcggta gagcagggga ttgaaaatcc ccgtgtcggt ggttcgattc
     8821 cgcctcgagg caccatcaat tcctccttag ttcagtcggt agaacggtgg actgttaatc
     8881 catatgtcgc aggttcgagt cccgcaggag gagccatatt ctgaagccct gatgagtaat
     8941 catcagggtt ttctttttga gctttccggc taactactaa gaaattgctc aatttttagt
     9001 attatgcgtt caaaaacgct aaa
//
LOCUS       DANLXF010000091         8913 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.091, whole genome shotgun sequence.
ACCESSION   DANLXF010000091 DANLXF010000000
VERSION     DANLXF010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8913
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.091"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..691)
                     /locus_tag="PXD66_001393"
     CDS             complement(<1..691)
                     /locus_tag="PXD66_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL6319688.1"
                     /translation="MWKNRQLFFGYAENRLLLLSRSITGLLGILCGVYIIDHLVLSDV
                     DMIGKLTSFILIILSAIFLKERASLLQWILCLIAFIGALFIIKPAVDIRFMPYLVGVI
                     GSVFAAIAYLCLRLLAKTKKVESPNTIVFFFSAFSTLILLPFVAIDYVVLTNLQTIYL
                     VLAGVTSAVGQFCVTTAYKYAAAKDISIYSYASVLFSAILGLLFFEQYPDMLSWLGYA
                     IIFVSSWLMFVL"
     gene            complement(927..1412)
                     /locus_tag="PXD66_001394"
     CDS             complement(927..1412)
                     /locus_tag="PXD66_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDL6319689.1"
                     /translation="MKKFAKVLMVAVPAFALAACSSSSDDAAANAAANAAANAAANAA
                     GEFGGLTAEQLKAQYNTVYFGFDSYAVEGEYRQLLDAHAQLLAKTQGNVTIAGHADER
                     GTPEYNIALGQRRADAVKGYLAAKGANNTSTVSYGEEKPAVLGHSEADYAKNRRAVLE
                     Y"
     gene            complement(1439..2725)
                     /gene="tolB"
                     /locus_tag="PXD66_001395"
     CDS             complement(1439..2725)
                     /gene="tolB"
                     /locus_tag="PXD66_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HDL6319690.1"
                     /translation="MKLFSRISGVIGIFSALLTTQVVADSDVRISIDEGVSMAQPIAV
                     VPFKASGVPADVAQIISDDLRNSGKFTPLERSQMPAQPGSAAEVVADQWSAIGIDNIV
                     VGQVSSTGGGYNVAYQLIDTLGTSGTAGGVIAQGSFIVPAAQIRQGAHTVSDEVFEKL
                     TQIRGAFRTKIAYVVQRGVSSYELRVSDYDGFNAFTITTSKEPLMSPEWSPDGSRLAY
                     VTFENKKAQVVVHDIRSGARRVVAALKGHNGAPSFSPDGSRIAFASNQDGELNIYVVG
                     SGGGTPSKLTANAGNNTEPSWSPDGSTIYFTSDRAGSPQVYRMSSSGGGASPMGGSGS
                     YNAKVSSDGKNLIMIAGDRVVKRDLASGGTEVLSSTFLDESPSISPNGIMVIYSSTKG
                     TSKVLQLVSADGRFKANLPGAGGHYKFPAWSPYLTK"
     gene            complement(2741..3964)
                     /gene="tolA"
                     /locus_tag="PXD66_001396"
     CDS             complement(2741..3964)
                     /gene="tolA"
                     /locus_tag="PXD66_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HDL6319691.1"
                     /translation="MKSQQDRLELAVIVSILLHILLIGLLLLGSLFTKTELAAAGGSG
                     GDSDSFEAVMVDTGQVAAEYGQLLAEKKGSKASKPKVEPKEEAVEEDVEDKPTPEEIQ
                     KQKQAELAAIQEKQRQQEIERQQKLQEQQKQEQAKQEELKKQQAEEATRKKAAEAAKL
                     KADAEAKRLEAAAKQAEEEKKAKEALEKKLEQQKKAEAEKKLKEQKELKEQQAKEAKE
                     AKEKAEKEAKAKAEKEAKEKAEKEAKAKAEKEAKEKAEKEAKAKAAAEAKAAQAKNNK
                     ALDDFLNSGDIGGGSSKGGNKNSAGSQGSGGTSGLGQGTNVDGNAYGQRIKKLIQSRY
                     RVDPSFAGKQCDVKIFLSRDGTITNYQVISGDKAVCDAAVSAIVATRKVPPAPSDAVY
                     NMFKSPTLDFSLKVK"
     gene            complement(3978..4415)
                     /gene="tolR"
                     /locus_tag="PXD66_001397"
     CDS             complement(3978..4415)
                     /gene="tolR"
                     /locus_tag="PXD66_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HDL6319692.1"
                     /translation="MSRRLKRNDIKSEINIVPFLDVLLVLLLIFMATAPIISQSVEVN
                     LPEDTHSQSVSNEDKTPVILEVAGVGEYKLKIDGNYVQSNGQENINESEVVAYAGEQF
                     QKDNNTLFLVAGDKVVPYEEIMKGITLLKDAGVKSVGLMTEGK"
     gene            complement(4557..5237)
                     /gene="tolQ"
                     /locus_tag="PXD66_001398"
     CDS             complement(4557..5237)
                     /gene="tolQ"
                     /locus_tag="PXD66_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HDL6319693.1"
                     /translation="MQTEFSLLSLFLEASIVVKIVMLILIVFSILSWAVIIQRSKILT
                     KAKKDSLAFEDRFWSGEDLNRLHQGLENRRDALNGSEQIFYVGFKEFTRLQQANPDAP
                     ESIIQGSTRAMNLALNREMESLENYIPFLGTVGSISPYIGLFGTVWGIMHSFMGLSAV
                     KQATLQSVAPGIAEALIATAIGLFAAIPAVMAYNRLNLRLGKVEQGYVNFIDEFTTLL
                     HRQAFSKR"
     gene            complement(5259..5660)
                     /gene="ybgC"
                     /locus_tag="PXD66_001399"
     CDS             complement(5259..5660)
                     /gene="ybgC"
                     /locus_tag="PXD66_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HDL6319694.1"
                     /translation="MDFPIRVYYEDTDAGGVVYHSNYLNFFERARTEFLRQQHFSQQE
                     LFSQSLAFVVKRIEIDYKLPARLDDLLNVETTLSELKKATLIFKQRLWKDGDCLSEAN
                     VTVACVDLIKMKPVAIPDDIRQALQKSYFSI"
     gene            complement(5823..6110)
                     /gene="ybgE"
                     /locus_tag="PXD66_001400"
     CDS             complement(5823..6110)
                     /gene="ybgE"
                     /locus_tag="PXD66_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HDL6319695.1"
                     /translation="MIHKLYNLTAKGLLKALSFILAAVLFATIWVNSTAFALSFGGKT
                     PYLAMLVFYGMAILWVHGIGFEIRAAIWKVIFLPLLGYLIVIPALWILLVK"
     gene            complement(6110..6208)
                     /locus_tag="PXD66_001401"
     CDS             complement(6110..6208)
                     /locus_tag="PXD66_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CydX/CbdX family cytochrome bd oxidase small
                     subunit"
                     /protein_id="HDL6319696.1"
                     /translation="MFYVTWVLGVLLAIFFATVITISIEKTGKFDE"
     gene            complement(6223..7356)
                     /gene="cydB"
                     /locus_tag="PXD66_001402"
     CDS             complement(6223..7356)
                     /gene="cydB"
                     /locus_tag="PXD66_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDL6319697.1"
                     /translation="MIDYEILRFIWWILIAVLLIGFAITDGFDMGVLNLLPIIGKSNV
                     ERRIMINTIAPHWDGNQVWLLTAGGAIFAAWPTVYATSFSGFYIAMVLVLAALFFRPV
                     GFEYRAKIDNPKWRSAWDWGLFIGGFVPSLVFGVAFGNLLQGVPFEFNNINQSQYTGS
                     FFGLLNPFALVCGIISLMMLTTQGATWLQMKTTDELHNNSRKVAQVTASIVLVAFILA
                     GVWLYFKDGFVITSELDHNAQSLLTNKTVAVQAGAWFRNYAEMPVLWVVPALVVVGAL
                     LTIIASKANRSGLAFFSSSLMIVGVILTAAVSMFPFIMPSITHPEMSLTVWDATASHT
                     TLTVMLFVAGIFVPLVLAYTIWSYIKMYGRLDASYLEENKAAY"
     gene            complement(7369..>8913)
                     /locus_tag="PXD66_001403"
     CDS             complement(7369..>8913)
                     /locus_tag="PXD66_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HDL6319698.1"
                     /translation="DVVELSRLQFALTALYHFLFVPLTLGLSFVLVVMETLYVTTGKE
                     VYKDMTKFWGKLFGINFALGVTTGITMEFQFGMNWSYYSHYVGDIFGAPLAIEGLMAF
                     FLESTFVGLFFFGWERLSKAKHLLATYCVAFGSNFSALWILVANGWMQNPIASEFNFE
                     TMRMELSSFSELVLNPVTQSKFLHTVTAGYVCGAIFVLGISSYYILKGRDLGFAKRSF
                     SVGASFGLIAIISVLIMGDESGYEIGKAQPTKLAAMEGEFETQPAPAAWNAFVIPNTA
                     EMKNEFSIHIPYAAGIIATRSLDKEIKGLKDIRVINEQRVRTGMVAYGLLEKLRSGNY
                     TEADKEAFKAVQKDLGFGLLLKQYTPNVVDATEEQIQKAADNTIPNVGPTFWAFRVMM
                     LSGFLMLILITTAFIQNMRGKVESSRFLLRGLLFGLPLPWIAIESGWFLAEYGRQPWA
                     IYEMLPVGVSNSALTTGDLWFTIGLLCGLYTLFLVVEMYLMFKYGRLGPSALKTGRYY
                     FEQSTK"
BASE COUNT         2806 a         1954 c         1488 g         2665 t
ORIGIN      
        1 ttaatacaaa cataagccag cttgagacaa aaataattgc ataacctagc caactcaaca
       61 tatccgggta ttgttcaaag aacaacaacc ctaaaattgc actaaatagc acagaggcat
      121 aactataaat tgagatgtct ttcgccgccg catatttata ggcggtagtt acacaaaact
      181 gcccgaccgc tgaagttacc cctgctaaca ctaagtagat tgtctgtaaa ttggttagca
      241 ctacataatc aatggcgaca aaaggcaata atatcaaggt cgaaaaggca gaaaaaaaga
      301 aaacaatggt attaggtgat tctacttttt tggtttttgc caataacctt aagcataaat
      361 aggcaatggc tgcaaacaca gagccaatca cgccaaccag ataaggcata aagcgaatat
      421 caactgccgg tttgataata aaaagtgccc cgataaaggc aataaggcat aaaatccatt
      481 gcaggagaga tgctctctct tttaaaaaaa tcgcagaaag aataattaaa atgaacgacg
      541 tgagtttgcc aatcatatcg acatcactta gcacaaggtg atcgatgata tatacaccac
      601 ataaaatccc caataacccg gtgatggaac ggcttaataa taacaaacga ttttctgcat
      661 aaccaaagaa taattggcga tttttccaca caatataacc gcttatcaat gctgtgcgtt
      721 gcgaaaaatc gctttttgtg caatcggtac atcccccgaa agacgaagaa aagcagccat
      781 aatcgcaaag cttagtgccg acaaaagcac acaaaaaatg gctttaaata acggcgagag
      841 cgaattccaa aaattcatag cataaaggct tttaataaaa ataaagccct gattattcag
      901 ggcttcaatt tagaatcaac taagaattag tattctaata ctgcacgacg gtttttcgcg
      961 tagtcagctt ctgagtgacc taatactgct ggtttctctt caccgtaaga aacagttgaa
     1021 gtgttgttag cacctttagc tgctaagtaa cctttaactg catctgcgcg acgttgacct
     1081 aatgcgatgt tgtactctgg agtaccacgc tcatcagcgt gaccagcaat agttacgtta
     1141 ccttgagttt ttgctaataa ttgagcgtga gcgtctaata attgacggta ttcaccttca
     1201 actgcatagc tgtcaaaacc gaagtataca gtgttgtatt gtgcttttaa ttgttcagct
     1261 gttaaaccac cgaattcgcc tgctgcattt gctgctgcat ttgctgctgc gtttgctgct
     1321 gcgtttgctg ctgcatcatc tgatgagctg ctacatgctg ctaatgcgaa agctggtact
     1381 gctaccatta atactttagc aaattttttc atttgttgct cctaatagag aaatattatt
     1441 atttagttaa gtatggtgac catgcaggga atttataatg tccacctgcg ccaggcaaat
     1501 tagctttaaa acgcccatct gcagacacca attgtagcac tttactcgta cctttggtag
     1561 agctataaat aaccataatg ccattcggtg aaatacttgg actttcgtct aagaaagttg
     1621 aactcagtac ttctgtgcca cctgaagcga gatcgcgttt cacaactcta tcaccggcaa
     1681 tcatgattaa attcttacca tcagatgata ctttcgcatt atagctaccg ctaccgccca
     1741 ttgggctagc accaccacct gatgaactca ttctataaac ttgaggagaa cctgcacggt
     1801 cagacgtaaa gtagattgtg ctaccatctg gagaccagct tggttcagta ttatttccag
     1861 cattagcggt caatttactt ggtgtaccac cgccactacc tacaacataa atatttaact
     1921 caccatcttg atttgaggca aacgcaatgc gagaaccgtc tggagagaat gatggcgcac
     1981 cattatgtcc tttcaatgct gccactacac ggcgagcacc tgaacgaata tcgtgaacaa
     2041 caacctgcgc ttttttattt tcaaatgtca cataagctaa tctgctacca tctggtgacc
     2101 actctggcga cattaaaggc tctttactgg ttgtaatagt aaaggcatta aaaccatcat
     2161 aatcagatac tcgtaattca tacgatgata cgccacgttg aaccacatac gcaattttag
     2221 ttctaaatgc accgcgaatt tgggttaatt tttcaaatac ctcatcactt actgtatgcg
     2281 caccttgacg aatttgggct gccggaacaa taaatgaacc ttgggcaatg acaccacccg
     2341 ctgtacccga tgtaccaaga gtatcaatta attgataagc gacattataa ccgccacctg
     2401 tgctactcac ttggccaact acaatattat caataccgat agcactccat tgatctgcca
     2461 ccacttccgc tgctgatccc ggttgagcag gcatttgaga gcgttcaagc ggtgtaaatt
     2521 taccactatt tcgcaaatca tcagaaataa tttgtgcaac atctgccggc acaccgctgg
     2581 ctttaaatgg cacaaccgca atcggctgag ccatacttac accttcatca atagaaattc
     2641 gcacatcaga atcagcaacc acctgagttg tcaataatgc tgaaaagatg cctattacac
     2701 ccgatatacg agagaataat ttcatttgta gtcctctaaa ttatttaact tttaaactaa
     2761 aatcaagtgt tggtgattta aacatattat ataccgcatc tgatggtgca ggtggtactt
     2821 ttctagttgc cactattgca cttaccgctg catcacatac tgctttatcg ccactgataa
     2881 cttgatagtt agtaattgtg ccatctcgac ttaagaatat cttaacatca cattgttttc
     2941 cggcaaaact tggatcaacc cgatatcgag attgaattaa tttcttaatt cgttgaccat
     3001 atgcattacc atctacatta gtaccttgcc ctaagccact tgtaccacca gaaccttgtg
     3061 atcctgctga gtttttgtta ccacctttac ttgaaccacc accgatgtct ccactattga
     3121 ggaaatcatc aagtgcttta ttgtttttcg cttgggcggc ttttgcctca gcagctgcct
     3181 tcgctttagc ttctttctcg gctttctctt tggcttcttt ttctgctttt gctttggctt
     3241 ctttctcagc cttttctttt gcttcttttt ctgccttggc tttagcttct ttttctgctt
     3301 tttccttcgc ttctttagct tcttttgcct gctgttcctt cagttctttt tgctctttta
     3361 actttttctc agcttccgct ttcttctgct gttcaagctt cttttcaagt gcttctttag
     3421 cttttttctc ttcttcagct tgtttagcag cagcctctaa acgcttagct tctgcatctg
     3481 ctttcagttt tgctgcttca gctgcttttt tacgagttgc ttcttctgct tgctgctttt
     3541 taagctcttc ttgcttagct tgttcttgct tttgctgttc ttgtagtttt tgctgacgct
     3601 caatttcttg ctgacgctgt ttctcttgaa tcgccgctaa ctcagcttgt ttctgttttt
     3661 ggatctcttc cggagtcggt ttatcttcca catcttcttc aacagcttcc tctttaggct
     3721 ctacctttgg cttcgatgct tttgagcctt ttttctctgc caataactgc ccatattcag
     3781 cagccacttg cccagtatct accatcacag cttcaaagct atcgctatcg ccaccactgc
     3841 caccagccgc agcgagttcc gtcttagtga aaagagaccc gagcaataac aacccgatta
     3901 acaatatatg caataagatc gaaacaataa ccgctagctc tagtctatct tgttgagatt
     3961 tcacacgtta tccttggcta tttaccttct gtcattaaac ctaccgattt cactccggcg
     4021 tctttaagta gtgtaatacc tttcataatt tcttcataag gtactacttt atcaccggca
     4081 actaaaaata aggtattgtt atctttttga aattgttccc ctgcataggc aacaacttct
     4141 gattcattaa tattctcttg cccattactt tgcacatagt taccgtcaat tttcaattta
     4201 tattcaccaa cacctgccac ctccaaaatc actggagttt tatcttcatt agaaactgat
     4261 tggctatgcg tatcttccgg taaattaact tctacacttt gactgataat tggggccgtt
     4321 gccataaaaa tcagcaataa taccaacaat acatcaagaa atggaacaat gttgatttct
     4381 gatttaatgt cattacgttt taaacgacga gacatatatt cctctttaca ggtgcttagc
     4441 accttaattg atctgaaata accggcacag tgtcccggtc tcgaataatg cggttacaag
     4501 cggtcttttt tacgaaaaac tttacaaatt ttcattaaaa atcaaccgct tgtagattat
     4561 ctttttgaaa aagcctgacg gtgtaataag gtcgtgaact catcaatgaa attcacatac
     4621 ccttgctcaa ctttacctaa acgtaaattc aaacggttat atgccattac tgcaggaatc
     4681 gccgcaaata aaccgatagc cgttgcaatt aatgcttctg caatacccgg tgcgactgat
     4741 tgtaaagttg cttgcttaac tgcacttaat cccataaagg agtgcataat tccccaaacc
     4801 gttccgaata aaccaatata tgggctgatt gaacccactg tgcctaaaaa cggaatgtag
     4861 ttttctaaac tttccatttc acggtttaat gctaagttca tcgcacgggt agaaccttga
     4921 ataatcgatt ctggcgcatc tgggttcgct tgttgtaaac gggtaaattc tttaaaccca
     4981 acataaaaga tttgctctga accatttaat gcatcacgac gattctctaa accttggtgt
     5041 aaacggttta gatcttcccc tgaccagaat cgatcttcaa aagcaaggga atcttttttg
     5101 gctttagtta aaattttgct gcgctgaata atcaccgccc aagacaaaat tgagaataca
     5161 ataaggatca gcatcacgat ctttaccaca atacttgctt ctaaaaataa cgaaagaaga
     5221 ctaaattcag tttgcattaa taaaactccg aatagttttt aaatagaaaa ataacttttt
     5281 tgtaatgctt gacgaatatc atcaggaatt gcaaccggct tcattttgat taaatcaaca
     5341 caagcgaccg taacatttgc ctcactcaga caatcgccat ctttccacaa tcgttgttta
     5401 aagataaggg ttgctttctt caattctgag agtgttgttt ccacatttaa taaatcatct
     5461 aaacgagcag ggagcttata atctatttca atcctcttaa caacaaaagc aagggattgg
     5521 ctaaaaagtt cctgctgtga gaaatgctgc tgtctcaaaa attccgttct tgcccgctca
     5581 aaaaaattca gatagttgga atgataaact actccgccgg catcagtatc ttcataataa
     5641 acacgaatcg ggaaatccat aagcattatt tctctcattc aaaatgaaaa aacgcccaaa
     5701 atttaggcgc aaattgtgag atattttagg atcagaaata ttgttatgca agtatatttt
     5761 ttggtaaatt gcaaaaatat tttttatcta tcaaaaataa aaatagagac ataaaaatgc
     5821 ctctatttaa caagtaaaat ccataatgcc ggaataacaa taaggtaacc tagaagtggt
     5881 aaaaaaatca ctttccaaat agcagctcgg atttcaaatc caattccatg tacccataaa
     5941 atagccatac cataaaatac caacattgct aaatacggcg tttttccacc aaaagaaaga
     6001 gcgaaagcgg tcgaatttac ccaaatagtt gcaaataata cagccgctaa aataaaagaa
     6061 agggctttta acaagccctt tgctgttaag ttatagagtt tatgaatcat tactcgtcaa
     6121 acttacctgt tttttcaatg ctgatcgtga tgactgttgc aaagaagatt gcaagcaata
     6181 cacctaagac ccaagttaca taaaacatat tgtgtctcct tattagtaag ctgctttatt
     6241 ttcttctaaa taactagcat ctaaacgacc atacattttg atatatgacc aaatagtata
     6301 tgctaatact aacggtacaa agataccagc aacaaataac attacagtta atgttgtgtg
     6361 gctagcagta gcgtcccaaa ccgttaagct catttctgga tgagtaattg acggcataat
     6421 gaatgggaac attgatactg cagcagttaa aatcacccca acaatcatca atgatgatga
     6481 gaagaatgct aaacctgaac gatttgcttt tgaggcaata atagtcagta atgcacctac
     6541 tacaactaat gcaggaacaa cccataatac aggcatttca gcataatttc taaaccatgc
     6601 accagcttga accgctacag ttttattcgt caatagagat tgtgcattat gatctaactc
     6661 gcttgtgatc acaaaaccat ctttaaaata taaccataca cctgcaagaa taaatgccac
     6721 taatacaata gaagctgtca cttgtgccac ttttcttgag ttgttatgta gctcatcagt
     6781 agttttcatt tgtaaccaag ttgcaccctg tgtggttaac atcattaagc tgataatacc
     6841 gcacactaat gcaaatggat ttaataaacc aaagaatgaa cccgtatatt gtgattggtt
     6901 aatattatta aattcaaatg gtacaccttg taataagtta ccgaatgcaa caccgaatac
     6961 taatgatgga acgaaaccac caataaataa tccccaatcc caagcactac gccatttcgg
     7021 attatcaatt tttgcacgat actcaaaacc tacagggcgg aaaaataatg ctgcaagcac
     7081 aagcaccatt gcaatataga aacccgagaa cgaagttgca tagactgttg gccatgcagc
     7141 gaagatcgca ccaccagcgg tcaataacca tacttggtta ccatcccagt gaggggcaat
     7201 tgtgttgatc ataatacgac gttcaacatt acttttacca atgataggta aaaggtttaa
     7261 tacgcccata tcaaaaccat cagtaattgc aaagccaatt aacaataccg caattaagat
     7321 ccaccaaata aagcggagaa tttcataatc gatcatttct aactcctatt atttagttga
     7381 ttgttcaaaa taataacggc ctgtttttaa tgcactaggg ccaagacgac catatttgaa
     7441 cattaaatac atttctacaa ctaagaatag tgtatataaa ccacacaata agccgattgt
     7501 aaaccataaa tcacctgttg ttaatgcaga gtttgacaca ccgacaggta acatttcata
     7561 gatagcccaa ggctgacggc catattcggc aaggaaccaa ccagattcaa tcgcaatcca
     7621 aggtaacgga agaccgaata acaagcctct taataagaaa cggctacttt ctactttacc
     7681 acgcatattt tgaataaatg ctgtcgtaat caagataagc atcaagaagc cagataacat
     7741 cattacacgg aatgcccaga atgtagggcc tacatttgga attgtattat ctgctgcttt
     7801 ttgaatttgt tcttcagtcg catcaaccac attaggtgta tattgtttta acaataaccc
     7861 aaagcctaaa tctttttgta cagctttaaa tgcttcttta tccgcctctg tataattgcc
     7921 agaacgtaat ttttctaata gtccgtaggc taccataccg gtacgaacac gttgttcatt
     7981 aattacacga atatctttta ggcctttaat ttctttatct aaagaacggg ttgcaataat
     8041 accagctgca taaggaatat gaattgagaa ttcatttttc atttccgcag tatttggaat
     8101 cacaaaagca ttccaagcag ctggagcagg ctgagtttcg aattcacctt ccattgcagc
     8161 taatttagtc ggctgagctt taccgatttc ataaccagat tcatcaccca taatcagtac
     8221 agagatgatc gcaattaaac caaaacttgc acctactgag aatgaacgtt ttgcaaagcc
     8281 taagtcacga ccttttaaga tatagtatga actaatacct aatacaaaaa tagcaccaca
     8341 aacatagcct gcggttacag tatgtaagaa tttagattgt gtaactgggt ttaatacaag
     8401 ttcagagaaa cttgaaagct ccattcgcat ggtttcaaag ttgaattcag aagcaatcgg
     8461 gttttgcatc caaccattag caactaaaat ccatagtgca gagaagttag aaccaaacgc
     8521 aacacagtaa gttgctaata aatgtttcgc tttcgataaa cgttcccagc caaagaagaa
     8581 caagcccaca aaggtagatt ctaagaagaa cgccattaaa ccttcaatcg ctaatggagc
     8641 gccaaaaata tcacctacat aatgagaata atatgaccag ttcataccaa actggaattc
     8701 cattgtaata ccagttgtta cccctagggc aaagttaata ccaaataatt taccccagaa
     8761 tttagtcata tctttatata cttctttacc cgtagttaca tacagggttt ccatcaccac
     8821 aaggacgaaa gaaagaccta atgtaagcgg tacgaataag aagtgataga gtgcagttag
     8881 agcaaactgc aaacgtgaga gttcaactac atc
//
LOCUS       DANLXF010000092         8766 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.092, whole genome shotgun sequence.
ACCESSION   DANLXF010000092 DANLXF010000000
VERSION     DANLXF010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8766
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.092"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            34..1290
                     /gene="pilQ"
                     /locus_tag="PXD66_001404"
     CDS             34..1290
                     /gene="pilQ"
                     /locus_tag="PXD66_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HDL6319699.1"
                     /translation="MSIVLKNAPTSLIFTYLAEETGKNMVLDDNIETKSTLRLENKSV
                     DEIFKTLGKVNKLSLTQEDDIVYIHKKEEKAADLTPLPIMNLQNNGQNTPLVTAPKLI
                     TKTLKLHYAKASEVIESLTKGSGTFLSENGYIHFDERSNSLIVKDSAKSLKNIENLVK
                     QLDQPTEQIAIEARIVTISSEHLQELGVRWGIFSRGAGHYKFGGRLEGNGLNNVTNNL
                     NVNFPITGGASAVLQVSSINSRVLDLELSALEQENSVEIIASPRLLTTNKKPASIKQG
                     TEIPYVMYNTKSEATDVEFKEAVLGLEVVPHLSTQNQILLDLIVTQNSPNSQSGSSGL
                     ITIDKQELNTQVFAKHGETIVLGGIFQHLTQKGEDKVPILGSIPFIKRLFSQTRDKIS
                     KRELVIFVTPYILQSNEKSSNRKKSK"
     gene            1371..1784
                     /gene="nusB"
                     /locus_tag="PXD66_001405"
     CDS             1371..1784
                     /gene="nusB"
                     /locus_tag="PXD66_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDL6319700.1"
                     /translation="MKISPRRRARECAVQAIYSWYISQNTVEEVELAFVTDQDMKGVD
                     MPYFRKLFRGVVDNVEAIDEALRPYLDRKESDVDPIERSILRLSTYELKFEHDVPFRV
                     VINEGIEVAKVFGSDDSHKYINGILDKLAPALGRK"
     gene            1847..2827
                     /gene="thiL"
                     /locus_tag="PXD66_001406"
     CDS             1847..2827
                     /gene="thiL"
                     /locus_tag="PXD66_001406"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HDL6319701.1"
                     /translation="MGEFDIIERYFNASQRPPRKDVLLSIGDDCALTSLKPNQQLAIT
                     TDTLVCGTHFLPTISPADLAYKSVASNLSDLAAMGAEPAWISLALTLPEINHDWLAEF
                     SEHFFEILDHYNVDLIGGDTTCGPLSLTITAQGIVPQDRGLFRHNAKVGDWIYVSGTL
                     GDSAGGLQLLLNQQSEQVNWNKEQSYLIQRHLRPTPRVLLGLTIASIANAAIDISDGL
                     LSDLSHILKRSDCGAVLNLENFPLSDVLLSCYSRTEAEYFALNGGEDYELCFTVPNDN
                     KAKLDKALANIGVTYTCIGQIRSAKEGLTLLRHNQPVTLPSQRGYDHFKK"
     gene            2832..3305
                     /locus_tag="PXD66_001407"
     CDS             2832..3305
                     /locus_tag="PXD66_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDL6319702.1"
                     /translation="MKPINLKNPIHFFAFGFGSGLLKPAPGTWGSLAGLIVAIALWSI
                     TQSALFFGLLTLVSFIAGCVICTKASQDLEVHDDGRIVWDEIVAIFLMFTCLPEYNLL
                     GYLLAFISFRIFDILKPFPIRYFDEKLESGLGIMFDDILAAIFALISLHLLYYFI"
     gene            3312..3935
                     /locus_tag="PXD66_001408"
     CDS             3312..3935
                     /locus_tag="PXD66_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HDL6319703.1"
                     /translation="MLTILFIHFAGLISPGPDFFYVVRQSASHSVKSGVLAATGISIG
                     IIFWAAFAIFGLAWLSNSIGSLFQYAIMIIGGCYLVYIGSQMVRVTENIIFVENKAQL
                     TPLNPWQEIKKGLLINIFNAKAGVYFTSVISAYVDNLSNTLQMFELLLLFVVSTFIYF
                     CVVALLFSRRPVRLFYAKYSRYIDNISGVIFILFGSMLIYEGFSHFI"
     gene            4047..4652
                     /gene="ruvA"
                     /locus_tag="PXD66_001409"
     CDS             4047..4652
                     /gene="ruvA"
                     /locus_tag="PXD66_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005686751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDL6319704.1"
                     /translation="MIGRLHGKIIEKQPPEILLDVQGVGYELLLPMTSFYSLPQIGEE
                     ATIFTHLVVREDAHLLFGFAQKQDRTLFRELIKTNGVGPKLALAILSAMSVSQFATAI
                     ENEELAKLTKIPGIGRKTAERLLVELKGKFKGMAQSDFFVEVSHEQIVASNTPDPANE
                     ALDALIALGYKPADAEKMIKKVNKSGATSEQLIREALKNSL"
     gene            4789..5793
                     /gene="ruvB"
                     /locus_tag="PXD66_001410"
     CDS             4789..5793
                     /gene="ruvB"
                     /locus_tag="PXD66_001410"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDL6319705.1"
                     /translation="MIEADRIISASPKREEEVIDRAIRPKLLADYVGQPSVHDQMEIF
                     IKAAKLREEALDHLLIFGPPGLGKTTLANIVANEMGVNIRTTSGPVLEKAGDLAAMLT
                     NLEPYDVLFIDEIHRLSPAIEEVLYPAMEDYQLDIMIGEGPAARSIKLDLPPFTLVGA
                     TTRAGSLTSPLRDRFGIVQRLEFYSVDDLTLIVKRSADCLNLNLSADGAYEVARRSRG
                     TPRIANRLLRRVRDYADVRNNGIITSDIAKQALAMLDVDSEGFDFMDIKLLQAIVERF
                     DGGPVGLDNLAAAIGEERDTIEDVLEPYLIQQGFLQRTPRGRIATSRTYAHLGITQID
                     "
     gene            complement(5892..6575)
                     /gene="artM"
                     /locus_tag="PXD66_001411"
     CDS             complement(5892..6575)
                     /gene="artM"
                     /locus_tag="PXD66_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HDL6319706.1"
                     /translation="MWWDYLVEISQGIGTSLQLTFVALIIAFILAIILTFILSLENRP
                     LKWLVNGFITLFTGTPLLIQFFLIYSGPGQFEWITESAAWVLLSDAWFCAMLALALNS
                     AAYSTLLFHGAVKAIPKGQWETCAALGLSRLDTLKILVPYALKRALPSYSNEIVLVFK
                     GTSLASTITIMDIMGYARQLYGTEYDALSIYGMAGVIYLVITGIMTIVLRKLESRVLA
                     FERFETEKA"
     gene            complement(6575..7246)
                     /gene="artQ"
                     /locus_tag="PXD66_001412"
     CDS             complement(6575..7246)
                     /gene="artQ"
                     /locus_tag="PXD66_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HDL6319707.1"
                     /translation="MSSDILNLLVNATLMTLGIALASLLIGMLLSIFFVVLETSKIAC
                     IRKPTSIIITLLRGLPEILVVFLIYFGLIQVIFLITDEFAELNAFWCGAIALGIIFAA
                     YASQSLRGAIKAIPVGQWESGTALGLSRAQVFVRIILPQVWRHALPGLSNQWLVLLKD
                     TALVSLIGVDDLMRQSELINANTHQPFTWYGIAALIYLAITLISQRVIKVLEGRFTRF
                     ERGVA"
     gene            complement(7246..7971)
                     /locus_tag="PXD66_001413"
     CDS             complement(7246..7971)
                     /locus_tag="PXD66_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDL6319708.1"
                     /translation="MKKLLLASAIAFATLSANAQETITFAMEPSYAPFETTNEKGEVI
                     GFDVDIAKALCEELKATCEFKTQSFDSLIAGLKFKNFDAIISGMDITEARQKQVAFTN
                     AYLENSVSYVAMKGKFTPETAKNVGVQNGTTFQQYLSKNAKQYNLKPYASLQSAVLDL
                     KNGRVDMVFGDTPVLAEWLKTEKELDFVGEKVVDKAFFGNGYGIAVNKSNTALLEKLN
                     SALATIKTNGKYEQIYNTWMAGK"
     gene            complement(7997..8731)
                     /gene="artP"
                     /locus_tag="PXD66_001414"
     CDS             complement(7997..8731)
                     /gene="artP"
                     /locus_tag="PXD66_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HDL6319709.1"
                     /translation="MAIRIQNVNFFYGSNQALFDINLEIEKGDTVVLLGPSGAGKSTL
                     IRTLNLMEVPQSGTLAIANHQFDLTAKTDSKAVSLLRREVGMVFQQYHLWPHLTVMQN
                     LIEAPMKVLGLSKEEAVARAKGHLARLRLSEFAERFPLHLSGGQQQRVAIARALMMQP
                     QVLLFDEPTAALDPEITAQVVDIIKELQSTGITQVIVTHEVGVARKVASKVVYMEKGT
                     IIEQGDASCFESPKTEQFAQYLSHSE"
BASE COUNT         2637 a         1872 c         1698 g         2559 t
ORIGIN      
        1 ttatgcggtt tttcattagc ctatgcgaat accatttcca tagttttaaa aaatgctcca
       61 acttcgctta tttttactta tttagcggaa gaaaccggta aaaatatggt gcttgatgat
      121 aatattgaaa ccaaatcaac actacgatta gaaaataaat cggttgacga gatttttaaa
      181 acgcttggca aagtcaataa attatcctta acgcaagaag atgatattgt ttatattcac
      241 aaaaaagagg aaaaagcggc tgacttaacg cctctgccaa taatgaattt gcaaaataat
      301 gggcaaaata caccgcttgt caccgcccct aagttaatca caaaaacctt gaaactacat
      361 tatgcaaagg cttcagaggt gattgagtcg ttaaccaaag gcagcggaac gtttttatcg
      421 gaaaacggtt atattcactt tgatgaaaga agcaatagcc tgattgtgaa agacagtgca
      481 aaatcgctta aaaatatcga gaatttagta aaacagctcg accaacccac cgagcaaatt
      541 gcgattgagg ctagaattgt cactatcagt agcgagcatt tgcaagagct tggcgttcgc
      601 tggggaattt tttctcgtgg ggctgggcac tataaatttg gcggacggct ggaaggcaat
      661 ggactcaaca atgtcaccaa taacttaaat gtaaattttc cgattactgg tggagcatct
      721 gccgtattac aggtttcgtc cattaatagc agagtgcttg atttagaatt aagtgcatta
      781 gaacaagaaa atagtgtgga aattattgcc agcccacgcc tgttaaccac caataaaaag
      841 ccggcaagta tcaagcaagg cacggaaatt ccttatgtga tgtacaacac caaatcggaa
      901 gccaccgatg tcgaatttaa agaagcggta ttagggcttg aagtcgttcc acacctttca
      961 acccaaaatc aaattctact cgatctgatc gttacccaaa attcgccaaa ttcccaatcc
     1021 ggcagtagtg gcttaattac gattgataaa caagagctaa atacgcaagt atttgccaaa
     1081 cacggcgaaa ccattgtact tggtggtatt tttcaacatt taacccaaaa aggcgaagat
     1141 aaagtgccga ttttaggctc aattcccttc atcaagcggt tatttagtca aactcgagat
     1201 aaaatcagca aaagagagct agtcattttt gtgacgcctt atatccttca atctaatgaa
     1261 aagagcagca accgcaaaaa atcaaaatag cacatcataa acgcaagcaa aaatagggga
     1321 agtgtgatag aatcagccgt cattttttga gttaaagatt tagaggtttt atgaaaattt
     1381 ccccacgtcg ccgtgcaaga gaatgtgcgg tgcaagccat ttattcttgg tatatttccc
     1441 aaaacacggt tgaagaagtg gaattggcgt ttgtgaccga tcaagatatg aaaggcgttg
     1501 atatgcctta tttccgtaaa cttttccgtg gcgttgtgga taatgtagaa gccattgatg
     1561 aagcattacg cccttattta gatcgtaaag agtcagatgt tgatcctatt gaacgttcta
     1621 ttttgcgttt atcaacttac gaattaaaat ttgaacacga tgtgcctttc cgtgtagtca
     1681 tcaatgaagg gattgaagtc gctaaagtgt tcggctctga tgacagccac aaatacatta
     1741 acggcatttt agataaatta gccccagcat taggcagaaa ataataagat tccccaattt
     1801 atacataaat tggggatttt gattataacg ctcaaggaga ttttttatgg gtgaatttga
     1861 tattatcgaa cgctatttta atgcttctca acgcccacct cgcaaagatg ttttactttc
     1921 tatcggcgat gactgtgcgc tcacatcact caaaccaaat cagcaacttg cgatcacaac
     1981 cgataccctt gtttgcggca ctcattttct accgactatt tcacctgccg atctcgctta
     2041 taaaagtgtg gcgtccaatt taagcgatct tgccgcaatg ggtgcagaac ctgcatggat
     2101 ctctttagcc ttaaccttac ctgaaattaa tcacgattgg ctagctgaat ttagtgaaca
     2161 tttctttgag attcttgatc actataatgt tgatttgatt ggaggggata ctacttgtgg
     2221 accactatct ttgacaatca cagctcaagg gattgtgcca caagatagag gcttattccg
     2281 ccataatgct aaagtaggcg attggatcta tgtttccggc acactcggtg acagtgcagg
     2341 tggtttgcaa cttctgctaa atcagcaaag cgagcaagtg aattggaata aagagcaaag
     2401 ctacctgatt caacgccatt tacgcccaac acctcgagtt ttacttggct tgactatcgc
     2461 ctctattgct aatgcagcga ttgatatttc agatggtcta ttgtctgatt taagccatat
     2521 tttaaagcgc agtgactgtg gtgcagtatt aaaccttgag aatttcccct tgtctgacgt
     2581 cttacttagt tgttattctc gaacagaggc agaatatttt gccttaaacg gtggcgaaga
     2641 ttacgaactc tgctttaccg tgccaaatga caacaaagcc aaattagaca aagcattagc
     2701 caatatcgga gtaacttata catgtattgg gcaaattcgt tcggctaaag aaggtttaac
     2761 cctattacgt cataatcagc ctgtcacctt gccctcacaa cgaggctacg atcattttaa
     2821 aaaataacgc aatgaaacca attaatctta aaaatccgat tcattttttt gcctttggct
     2881 ttggttcagg cttattaaag cctgctcccg gcacctgggg tagcctcgcc gggctaattg
     2941 tagcaatagc cttgtggtct atcacacaat ccgccctgtt tttcggctta ctcaccctag
     3001 tttcttttat tgccggttgt gttatttgca ccaaagccag ccaagatcta gaagtacacg
     3061 atgacgggcg aattgtttgg gacgaaatag ttgccatctt tttgatgttc acctgtttgc
     3121 ctgaatacaa tcttcttggc tacctgctcg cctttatcag ctttcgcatt tttgatattt
     3181 tgaagccttt tccaatccgc tattttgatg aaaaattaga aagcggttta ggcattatgt
     3241 ttgatgatat tctcgcagcc atttttgccc tgatttcact tcatctttta tattatttca
     3301 tttaaaaatt tatgctcact attttattta ttcattttgc cgggcttatc agccccggcc
     3361 ccgatttctt ctatgtcgtc agacaatccg ccagccactc agttaaaagt ggggtacttg
     3421 ccgcaaccgg aatctcaatc ggcattattt tttgggcagc ctttgcgatt tttggtttag
     3481 cgtggctcag taatagtatt ggctcgctgt ttcaatatgc cattatgatt atcggtggct
     3541 gttacctggt ttatattggc tctcaaatgg tacgggtaac cgaaaatatc atttttgttg
     3601 aaaataaagc tcaactcacg ccgctaaacc cttggcagga aattaaaaaa ggcttattaa
     3661 tcaatatctt taatgccaaa gctggcgttt actttaccag cgtcatttca gcctatgtag
     3721 ataatttaag caatacccta caaatgttcg aactacttct actctttgtg gtaagtacct
     3781 ttatttattt ctgtgtggtg gcactactct tttctcgccg accggtcaga ctgttctatg
     3841 ccaaatatag tcgctatatt gacaatatct caggcgtaat tttcatctta ttcggctcga
     3901 tgctgattta tgaaggattc agccacttta tctaatcaca agcggtctga tttcactaaa
     3961 aatttgcaaa ttccgctatc aataaatagt gttattttat acagtttctg ctatgctact
     4021 gccaatttta actctgagag aacaaaatga ttggacgatt acacggcaaa attattgaaa
     4081 agcaaccgcc tgaaatttta ttagacgtgc aaggggtagg ctatgaacta ctgctcccga
     4141 tgaccagttt ctacagcctg ccacaaattg gcgaagaggc gactattttc acccatttag
     4201 tggtgcgaga agatgctcac ttgctattcg gctttgccca aaaacaagat agaactctat
     4261 tccgtgaatt aattaaaacc aacggtgtcg ggccgaagct cgcgttggca attctatcag
     4321 caatgtcggt gtcgcaattt gccaccgcga ttgaaaatga agagctggca aaattaacca
     4381 aaattccggg aatcggtcgg aaaaccgcag aacgcttatt ggtggaactc aaaggcaaat
     4441 ttaaaggaat ggcacagagt gatttctttg tggaagtatc gcacgagcaa attgtggcaa
     4501 gtaatacgcc agacccggca aatgaagccc ttgatgcctt aatagcccta ggctacaaac
     4561 ctgccgatgc ggaaaaaatg attaagaaag tgaataaatc cggtgcaacg agcgaacagc
     4621 ttattcgtga ggcgttaaaa aattcattat gatattgata gtatagggcg aaagatattt
     4681 cgcccctaca atctcatttt taaaaatata tgtgtagggg caaatcaata tttgccctca
     4741 tgagtaatat agggtaatac cattccacta caacaataat ctaaatgtat gattgaagca
     4801 gatagaatca tcagtgcctc gcctaagcgt gaggaagaag ttatagaccg agcaatccgc
     4861 ccgaaactgc ttgccgacta tgtcgggcag ccttctgtgc atgatcaaat ggagatcttc
     4921 atcaaagccg ctaaattgcg ggaagaggca ttagatcact tgctcatttt cggcccgccc
     4981 ggtttgggga aaactaccct tgccaacatt gtggcaaacg aaatgggggt gaatattcgc
     5041 acaacttcag ggccggtgct ggaaaaagca ggcgatttgg cagcaatgct gaccaacctt
     5101 gaaccttacg atgtgctgtt tattgatgag attcaccgcc tttctccagc gattgaagaa
     5161 gtgctttatc ctgcaatgga agattaccag ctcgatatta tgattgggga aggacctgct
     5221 gctcgttcca tcaagctcga tctaccgcct tttacccttg taggggcaac cacccgtgcc
     5281 ggctcactga cctcaccgtt acgagatcgt tttgggattg tacaacgctt agaattttac
     5341 tcagtggacg atcttactct gattgtcaaa cgcagcgccg attgcttaaa tctcaatttg
     5401 tcggcagacg gtgcttatga agtggctcgc cgctcccgtg gcacaccccg tattgccaac
     5461 cgcttactcc gccgagttcg ggattacgcc gatgtgcgta ataatggcat tattacctca
     5521 gacattgcca aacaagctct ggcaatgctc gatgtggatt cggaaggctt tgattttatg
     5581 gacattaaac tgttgcaagc gattgtagaa cgctttgatg gcgggccagt tggattagac
     5641 aatcttgcgg cagctatcgg cgaagaacga gatacgattg aagatgtgct agagccttat
     5701 ctgattcagc aaggctttct acaacgcaca ccaagaggca gaattgctac cagcagaacc
     5761 tatgcccatt taggtataac ccaaatagat tagtcatatt tctaaaggca ttgatacata
     5821 aataaaaagc ggatagttag tactatccgc tttcatttgc aaaaaatcaa taaaatttga
     5881 ccgcttatcc gctaggcttt ttcagtctca aaacgctcaa aagctaatac tctgctttct
     5941 aattttctga gaacgattgt cataatgccg gtaattacca agtaaataac ccctgccata
     6001 ccataaattg ataatgcgtc atactctgta ccataaagct ggcgagcata gcccataata
     6061 tccataatcg taatggttga agcaagcgat gtgcctttaa aaactagcac aatctcatta
     6121 ctataagaag gtaaagcacg ttttaatgcg taaggcacta aaattttcag tgtatctaaa
     6181 cggctcaaac caagggctgc gcaagtttcc cattgcccct tcggaatcgc ttttaccgca
     6241 ccgtggaaca acaatgtcga ataggcagca ctgtttaacg ctaaagccaa cattgcacag
     6301 aaccaagcgt cggataataa tacccaagcc gcactttctg taatccattc aaattgcccc
     6361 gggccactat aaatcaagaa aaattgaatt aataaaggcg ttccggtaaa aagcgtaata
     6421 aatccgttta ccaaccactt taacggacga ttttctaacg agagaataaa cgtaagaatg
     6481 atcgctaaaa taaaggcaat gatgagcgcc acaaaagtga gctgtaggct ggtgccaata
     6541 ccctgtgaaa tttcaactaa ataatcccac cacattaagc aactcctcgc tcaaaacggg
     6601 taaatctacc ttctaatact ttaatcactc gctggctaat tagagtaatc gctaaataaa
     6661 taagtgctgc tatcccatac caagtaaaag gttgatgagt attagcatta attaactccg
     6721 attgacgcat taaatcatct accccaatta acgataccaa tgccgtatct tttaacagca
     6781 ctaaccactg attgcttaag cctggtaaag cgtgtcgcca gacttgaggg agaataatgc
     6841 gaacaaatac ttgcgctcgg cttaacccta acgccgtacc agattcccac tgcccaactg
     6901 gaatagcttt aattgcacca cgtaaagatt gggaagcata agcagcaaaa ataataccca
     6961 gcgcaatagc cccacaccag aaagcattta actccgcaaa ctcatcggtg atcaagaaaa
     7021 taacttggat aagaccaaaa tagatcaaaa ataccactaa aatctccggc aacccacgca
     7081 ataatgtaat tattatacta gttggtttac ggatacatgc aatcttactg gtttcaagta
     7141 ccacaaaaaa gattgacaat aacattccaa tcaagaggct tgccagcgca atccctaagg
     7201 tcatcagggt tgcgttgaca agtaagttta aaatatcgct agacattatt tcccagccat
     7261 ccaagtatta taaatttgct catatttacc atttgttttg atggtagcta aagccgaatt
     7321 taatttttca agcaatgcag tgttagattt atttaccgca ataccatatc cattaccaaa
     7381 aaaggcttta tcgactactt tttcacccac gaaatctaac tctttttcgg tttttagcca
     7441 ctctgccaat actggtgtat caccaaatac catatcaaca cgtccatttt ttaaatctaa
     7501 cactgctgat tgtaggctag cgtaaggttt taaattatat tgtttggcat ttttgcttaa
     7561 gtattgttga aaagttgtac cattttgcac gccgacattc ttagctgttt ccggtgtaaa
     7621 tttccccttc attgcgacat aacttactga gttttctaaa taagcattgg taaaggcaac
     7681 ctgtttttga cgcgcttcag taatatccat gcctgaaata attgcatcaa aatttttaaa
     7741 ttttaatccc gcaattaagc tatcgaatga ttgcgtttta aattcgcaag ttgcttttag
     7801 ttcttcacat aatgcttttg caatatctac atcaaagcca ataacttcac ctttttcatt
     7861 tgttgtttca aaaggggcgt aagaaggctc catcgcaaaa gtgatagttt cttgtgcatt
     7921 ggcagaaagc gttgcaaatg cgatagcgga agctaataat aattttttca ttgttgtgtc
     7981 cttttgttgt tatagattac tctgagtgag ataaatattg agcaaattgc tctgttttcg
     8041 gtgattcaaa acaacttgca tcaccttgtt caataattgt acctttttcc atgtacacca
     8101 ctttgcttgc caccttacga gcaacgccta cttcgtgagt cacaatcacc tgagtaatgc
     8161 ctgtagattg caattcttta ataatatcaa ctacttgtgc agtaatttcc gggtcaagtg
     8221 ctgcggtagg ttcatcaaat aataagacct gtggctgcat catcagtgct ctggcaatcg
     8281 ccacccgttg ttgctgacca cctgaaagat gcagtgggaa acgctcggca aactcgctca
     8341 aacgcaaacg tgccaaatgt ccttttgctc tggcaaccgc ttcttctttg cttaacccaa
     8401 gcactttcat cggggcttca attaagtttt gcattacggt taaatgcggc cataaatgat
     8461 actgctggaa caccattccc acctcacggc gtaacaaact caccgctttg ctgtcggttt
     8521 tagcagttaa atcaaattga tgatttgcaa tcgccaaagt gcctgattgc ggtacttcca
     8581 ttaagtttaa ggttcgaatt agggtacttt tacctgcccc actcgggcca agcaatacaa
     8641 cagtatcccc tttttcaatc tctaagttaa tgtcaaataa ggcttggttt gagccataaa
     8701 aaaaattgac attttgaata cgaattgcca ttcgttaaaa tcctactaca tttaaaataa
     8761 tgaatg
//
LOCUS       DANLXF010000093         8659 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.093, whole genome shotgun sequence.
ACCESSION   DANLXF010000093 DANLXF010000000
VERSION     DANLXF010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8659
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.093"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            45..710
                     /locus_tag="PXD66_001415"
     CDS             45..710
                     /locus_tag="PXD66_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HDL6319710.1"
                     /translation="MNFTTYFSDENKRRSLILLSLFHIFIIAISNYLVQISFEVQVPL
                     TEFSFPTTWGTLTFPFIFLATDLTVRVFGADLARKIIFVVMFPALVISYVISVLFFET
                     QFQGFSALTEFNFFVFRIALASFAAYLVGQLLDVLVFNSLRQGKRWWLAPTCSTIFGT
                     LIDTFVFFAIAFYKSSDEYMAEHWFTIGSVDYAFKLFVSLLLFLPLYGVILNFIMKKL
                     DLK"
     gene            796..1599
                     /gene="map"
                     /locus_tag="PXD66_001416"
     CDS             796..1599
                     /gene="map"
                     /locus_tag="PXD66_001416"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDL6319711.1"
                     /translation="MNNIPLRNEEEIQKLRVACKLASDVLVMIAPYVKEGVTTGELDK
                     ICHEYILANGATSASLGYHGFPKSVCISLNEVVCHGIPSDDKKLKKGDILNIDVTVIK
                     DGYYGDNSMMYVVGEPSVRDKRLMAVTLESLYVGIRTVKAGVRLKEIGTAIQKYVEKE
                     GFSVVREYCGHGIGTEFHCDPQVVHYATDDGGVVLKEGMVFTIEPMVNAGKKEVRLMG
                     DGWTVKTKDRSHSAQYEHQIVVTKDGCEVLTIRDEEIAEGRISRIMHNS"
     gene            complement(1715..3129)
                     /locus_tag="PXD66_001417"
                     /pseudo
     CDS             complement(1715..3129)
                     /locus_tag="PXD66_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605131.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sucrose transporter subunit IIBC"
     gene            complement(3436..4086)
                     /gene="thiQ"
                     /locus_tag="PXD66_001418"
     CDS             complement(3436..4086)
                     /gene="thiQ"
                     /locus_tag="PXD66_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein"
                     /protein_id="HDL6319712.1"
                     /translation="MIELDLHFTYEQMNMHFQLSVKKGERIAVVGESGAGKSTLLNLI
                     AGFEKAESQQSQLWLNQKNHLDTEVAQRPVSMLFQDNNIFPHLTVEQNIGLALVPSLS
                     LNPKQKAQVKQIATQMGIETFLQRQADQLSGGQKQRVALARTLLRDKPILLLDEPFSA
                     LDPQRRAELQQIVFDICQSRNLTLLMVTHQLSESLFLFNRVITVADGEIVSDLSPL"
     gene            complement(4083..5657)
                     /gene="thiP"
                     /locus_tag="PXD66_001419"
     CDS             complement(4083..5657)
                     /gene="thiP"
                     /locus_tag="PXD66_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease"
                     /protein_id="HDL6319713.1"
                     /translation="MLSTLTKTTAWFIYFSIIALYVFSLWALMDYHQNDTLFVFSDIT
                     ATVAYSLLQASLSAVISLFLGSLLARSFFYLDFKGKNLLYKTISFIWALPSLVIIFAV
                     IGVFGNSGWLAKLFQQLGWNWQFNLYGLHGIILAHCLFNIPFVMKYYTGGLQLTPSSR
                     FQLAAQLNLTGWNYIQIVELPIIKTLLPYMFMTVFLVCFTSFPIVLMLGGSPKYSTLE
                     VAIYQAVTFEFDFAKALILIGVQFVVGIGLQLVMNYISTSALKKLKQQPNRVEIWKPK
                     PTGLAKLGLQAVIFLQSFAIILPLVSVIWAGLSVSKLSERLLNPTLWQATQYSLYLSL
                     IASIFVITISYLIALEARQLLYTKAKLRYSILTAITLFPLLLPIFLLSVGLFILLMNS
                     ELSSEALLLLVGICNGLTLLPFIYPMLFSAMWNSLTSHDKLAQGLGLKGLRRWWIVEK
                     NQLIRPLVTVFALAMSSSLGSFTVIAFFGNADFSSLPYLLYQQLGSYRTEDAAVTALI
                     LMLFALLPFLFSKDKL"
     gene            5918..6175
                     /gene="ptsH"
                     /locus_tag="PXD66_001420"
     CDS             5918..6175
                     /gene="ptsH"
                     /locus_tag="PXD66_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HDL6319714.1"
                     /translation="MYSKDVVITAPNGLHTRPAAEFVKAAKGFASDITVTSGGKSASA
                     KSLFKLQTLGLTQGTTITISADGEDEQKAVDFLVDLIPTLE"
     gene            6297..8018
                     /gene="ptsI"
                     /locus_tag="PXD66_001421"
     CDS             6297..8018
                     /gene="ptsI"
                     /locus_tag="PXD66_001421"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HDL6319715.1"
                     /translation="MITGIAASPGAVFGKALVLKEEPIVLNTRKITESEVEAEKAKFF
                     TGREKAAAQLTAIKEKARRTLGEEKEAIFEGHLMILEDEELEEEILSYLTDNLVTADV
                     AASKVIEMQASMLAEIDDEYLKERAGDIRDIGNRLVRNILNMNIVDLGDIQDEVILVA
                     YDLTPSETAQLNLDKVLGFITDVGGRTSHTSIMARSLELPAIVGTNNVTSLVKTGDFL
                     ILDAVNNEVHLNPSEEKIANFKALQEKLNAEKEELAKLKELPAQTLDGHKVDVAGNIG
                     TIRDVEGVLRNGGEAVGLYRTEFLFMDRTQLPGEEEQFQAYKEIVEAMNGQMVILRTM
                     DIGGDKELPYLDLPKEMNPFLGWRAVRIGFTRREILDTQLRAVLRASAFGNLAVMFPM
                     IISVEEIRLLKSIVAELKDQLRAEGHAFDENLKLGIMVETPSAAVNARHLAKEADFFS
                     IGTNDLTQYTLAVDRGNEIIAHLYNPLSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     VRATALLLGMGLDEFSMSGISVPHVKKLIRSINFADAKALADEALAQPTAADIERLVD
                     EFYQKLN"
     gene            8103..8603
                     /gene="crr"
                     /locus_tag="PXD66_001422"
     CDS             8103..8603
                     /gene="crr"
                     /locus_tag="PXD66_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005702996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL6319716.1"
                     /translation="MGLFDKLFGSKQKESQEIKIYAPLTGEIVNIEDVPDVVFSEKIV
                     GDGVAIRPSGDTIVAPVNGTIGKIFETNHAFSIESEEGVELFVHFGIDTVELKGEGFT
                     RLAQEGQTVKVGEPIIKFDLALLEGKAKSVLTPIVISNMDEISNLSKLNGQVVAGESV
                     VLTLTK"
BASE COUNT         2678 a         1557 c         1862 g         2562 t
ORIGIN      
        1 agttccctta cctcaagttt tcatttaacg aaaaaggata caatatgaac tttactactt
       61 atttttcgga tgagaacaaa cgtcgttctc taattttact ttcacttttc catattttta
      121 tcatcgcaat tagcaactat ttagtgcaaa ttagctttga agttcaagta ccactaacag
      181 aatttagctt tccgacaact tggggaacgt taacattccc atttattttc ttagcgaccg
      241 atttaaccgt gcgtgtattt ggtgcagatc ttgctagaaa aattattttt gttgtgatgt
      301 ttccggcact tgtcataagc tatgtgattt ccgttttatt ctttgaaacc caatttcaag
      361 gctttagtgc attaactgag ttcaactttt ttgttttccg tattgcactg gctagctttg
      421 cagcttattt agttgggcaa ttattagacg ttctagtatt taatagttta agacaaggta
      481 agcgttggtg gcttgctcca acctgttcaa ccatttttgg tactttaatc gatacttttg
      541 tattctttgc tatcgccttt tataaaagta gcgatgaata tatggcagag cattggttta
      601 ctattggttc ggtcgattat gcgtttaaac tctttgtgag cctactgcta tttctaccgc
      661 tttatggtgt gatattaaat ttcattatga agaaacttga tttaaagtag aaatctaaac
      721 tgaggtttgg ggattgtgct gaaaaaaggt ataatcccct tactttttat ggcagatata
      781 gcagagagca atgtaatgaa taatattcca ttaagaaacg aagaagaaat tcaaaaacta
      841 cgagtagcct gtaaactggc ttcagatgtt ttagtaatga tcgcccctta tgtgaaagag
      901 ggcgtaacaa ctggtgaatt agataaaatt tgtcacgaat acattttggc aaatggggca
      961 acttcagcca gtttgggcta tcacggcttc ccaaaatcgg tgtgcatttc gttaaatgag
     1021 gtcgtttgtc acggtattcc aagtgatgac aaaaagctga aaaaaggcga tattttaaac
     1081 atcgatgtga cggttatcaa agatggctac tatggcgata actcgatgat gtatgtagtc
     1141 ggtgagccaa gtgtgcggga taaacgctta atggcggtta cgctagagtc cctttatgtc
     1201 gggattcgca ccgtgaaagc gggggtgcgt ttaaaagaaa ttggtacagc gattcaaaaa
     1261 tatgtggaaa aagaaggctt ttcggtagta cgagaatatt gcggacacgg tattggtact
     1321 gaattccatt gcgacccaca agtagtgcat tatgctaccg atgatggcgg tgtagtgtta
     1381 aaagaaggaa tggtgtttac gattgagcca atggtgaatg ccggcaaaaa agaagtccgc
     1441 ttaatgggtg acggttggac agtgaaaacc aaagaccgca gccactccgc acaatatgag
     1501 catcaaattg tagttaccaa agacggctgt gaagtgctga caattcgtga tgaagagatt
     1561 gccgaagggc gtatttctcg aattatgcac aacagctaat ttgcaaattt ttagcgaaaa
     1621 atgaccgctt gcgaaactca ttgttaagct aaggcgttta aaaacaaaag ggattaagct
     1681 aacacttaat cccttttttg tgatctattt ttgattattt cgcttgtgct tttttcaccc
     1741 aaatggctga aatggtaaat gcaactacaa atgaaattac cataccgaca gaatacattg
     1801 caaggctgtc aggtttaatc gacggaatac ctaagaaacc ggctgcacct aaggcaattg
     1861 ctttcacatt aaagaaagcg ataaatgcac tggctaaacc tgaaccgatc atcgccgcaa
     1921 taaaggcttg gcggtagcgt aaatttactc cgaacattgc cggttgatac ctaacagggc
     1981 tgaaatacct gacggcacgg ctaaaccacg cactttggca tctttcattg caaaagctac
     2041 acctaaacag gctgcacctt gtgcaatgtt agacattgct gcaatcggga agataaatgt
     2101 accgccggtt tgtgctactt cagctaataa ttgggtttca accgcaataa aggtttggtg
     2161 cataccggta atcacgattg gagcgtagaa tgtaccgaaa attgctccac cgataaagcc
     2221 taagctgtca tataaccacg ttaagcctgc tgaaatcgct gaacctgctt cacgacccaa
     2281 tggtccgatt acagtgaaag ctaaaatacc ggcgataaat aatgacatca ttggcgtcac
     2341 caagttatct aaatagcttg gcacaaattt acggaaagtt ttctcaaggg ttgctaacac
     2401 ccaagccgaa acgatagttg gaataaccgt gccttgataa cccacttttt caatttctaa
     2461 gccgaaaata ttccagtatt tgatgttacc ttccattaag gttttggcat agttccaacc
     2521 gtctgcaagt gccgggtgaa ccaataacat accaagtgcc gcacctaaga atggattacc
     2581 gccaaatttg cgtgtagctg agaaaccgag taatacaggt aagaatacaa atggtgcgtt
     2641 agcaatagtg ttgatgaaat caactaaatc ggaaatattc ggataacggc taacaacaga
     2701 ttcaccttcc cagaagaaac cgactgccgt tagcattgag tgaataccca ttaacaaacc
     2761 gccagccaca atagccggaa tgatcggcac gaaaatatcg gctaagcctt ttaccaaacg
     2821 ttgtagtaat ggctgatttg ctgcaccggc agccgctact tcagaggtgg acatatctgc
     2881 catacccatt tgtttttgca tttgggcata cacttcattt accgtgcctg atccaaaaat
     2941 aatttggtat tgaccgggag tagagaattg acctttcacg ccttcaatat tttcaatcgc
     3001 ctctttatca attttggatt catcagcaat ggttaagcgt aaacgggttg cacagtgggc
     3061 aagtgtggta atgttttctt tgccacctaa ttttgcaatc gtttgttctg caatttttgg
     3121 atagttcatt tgaatttacc tttcataaaa aataatcggt tgcattttaa ctaaatcggt
     3181 ttagctaaaa aagagaagat ttaaaatatt tccaaatttg tgaagtagat cacattttgg
     3241 ggaagagaat agcggaatag ggaggtaata ttagttacag ttcccctctt tgctaaagta
     3301 gggggcgagt tctgaaaaaa atgaataacg ctagtatccg tgcggaacat cattcaccgt
     3361 tagattgttg gaattgacat cgttgcgtac gccagcgtgg cgtccctaca tttagaacct
     3421 agcagatctg ctttgctaaa gaggggagag atcagaaaca atctccccat ctgctaccgt
     3481 tatcacccga ttaaatagaa ataggctttc ggatagttgg tgagttacca ttaacagggt
     3541 tagatttctg ctttggcaaa tgtcaaacac gatttgctgt aattcggctc ggcgttgcgg
     3601 gtcgagggcg gagaagggtt cgtccaataa taaaatcggc ttatcccgca acaaagtacg
     3661 agctaatgcc actcgctgtt tttgtccgcc cgatagctga tcggcttgac gctgaaggaa
     3721 ggtttcaatc cccatttgtg tggcaatttg tttaacctgt gctttttgtt tcggattgag
     3781 cgacaaactt ggtactaaag ctaagccgat gttttgttct accgttaagt gcgggaaaat
     3841 attgttatct tggaatagca ttgaaacagg acgttgtgca acttcggtat caagatgatt
     3901 tttctgattt agccacagct ggctttgctg actttctgcc ttttcaaaac cggcaattag
     3961 attaagtaat gtgcttttgc ctgcgccact ttcgccaacc actgcaatac gttcgccttt
     4021 tttgacagaa agctgaaagt gcatattcat ttgttcgtaa gtgaaatgga gatccagttc
     4081 gatcatagtt tatccttgct gaataagaat ggtagtaacg caaataacat cagaatgagt
     4141 gcggtaacgg cagcatcttc ggttctatag ctaccaagtt gttggtagag taaataaggg
     4201 agtgagctga aatcagcgtt gccgaaaaaa gcaatgacgg tgaaactgcc taagctcgat
     4261 gacattgcta acgcaaatac ggtaacaagc ggtcgaatta gttgattttt ttccacaatc
     4321 caccaacggc gaagcccctt tagcccaagt ccttgtgcta atttgtcgtg cgaggtgagg
     4381 ctattccaca ttgcggagaa aagcattggg taaataaatg gcagtaatgt taagccgttg
     4441 catatcccga ccagtagcag taatgcctcg cttgagagtt cactattcat taataaaata
     4501 aacaaaccaa ccgataataa aaagatcggc agaagtaagg ggaatagggt gattgcggtg
     4561 agaatgctat atctgagctt ggcttttgta tagagtaatt ggcgagcttc aagtgcaatc
     4621 aaatagctga ttgtgattac gaatatagaa gcaattaaac ttaagtataa agaatactga
     4681 gtagcttgcc ataaagtagg gttgagcaaa cgctcactga gttttgacac gcttagtcct
     4741 gcccaaatga cgctgacaag cggtagaatt attgcaaaac tttgcaaaaa aataaccgct
     4801 tgtaagccga gtttagccag tccggtaggc tttggtttcc aaatttctac tcggttaggt
     4861 tgctgtttta gctttttgag tgcagaagtc gagatataat tcattactaa ttgtaagcca
     4921 atgcccacta caaattgtac cccaattaag atgagggctt ttgcaaaatc aaattcaaaa
     4981 gtgaccgctt gataaatggc aacttcaagc gtgctgtatt ttgggctacc gcctaacatt
     5041 aatacgattg gaaaactggt aaagcagacc agaaaaacag tcataaacat ataaggcagc
     5101 aaagttttga ttatcggtag ttccacaatt tggatataat tccaacctgt taggtttaat
     5161 tgagccgcaa gctgaaagcg actgcttgga gtcaattgaa gccctccggt ataatatttc
     5221 atcacaaacg gaatgttaaa caggcagtga gccaaaatga tgccgtgcaa gccgtataaa
     5281 ttaaattgcc aattccaacc gagttgttgg aaaagtttgg ctagccagcc tgagttacca
     5341 aatacgccaa ttaccgcaaa aataattact agtgagggta atgcccagat aaacgaaatg
     5401 gttttataga gtaaattttt tcctttaaag tccaaataaa agaaagatct tgccagtagg
     5461 ctgcctaaaa atagtgaaat taccgcagag aggctggctt gaagtaggct ataagcgacc
     5521 gtggccgtta tatcggaaaa aacaaataag gtatcatttt ggtgatagtc cattaacgcc
     5581 catagactaa aaacatagag agcaataata ctaaagtaga taaaccatgc ggttgttttg
     5641 gttaatgtgc taagcatatt gctccaaatt tagaatataa gtgaaagaag tggtagcgaa
     5701 attattgtaa catttccgct taagtgtgtg ctagaatctt gatattcgac aaatgatttt
     5761 taagttagct taaaaaacac cctatttagc agggtgatac gacgagtttt tgcgcttttt
     5821 agcgtgtttt taatttgaat ttgtagtaaa gttgcgtgcg caaaataaat ggattaaaac
     5881 aaattttttt aactttcaaa tttaagagga agattttatg tactcaaaag atgttgttat
     5941 taccgctcca aatggtttac acactcgccc agcggctgaa tttgtaaaag cagctaaagg
     6001 ttttgcttct gatatcactg tgacttcagg tggtaaaagt gcaagtgcaa aaagcttatt
     6061 caaattacaa acattaggtt tgactcaagg cacaacaatt acaatttctg cggacggcga
     6121 agatgagcaa aaagcagttg atttcttagt tgatttgatt cctactttag aataattttt
     6181 ctattctcgg atttaagatt tatgtcactt tattgtagct taataagcta caataaagtg
     6241 ttttgctatg taacatccag atgattttta catcatctta aattaaggac acaattatga
     6301 ttacaggtat cgcagcttcc cctggtgcag tatttggaaa agcattggtt ttaaaagaag
     6361 agccgattgt actaaatact cgcaaaatta ctgaaagcga agttgaggca gaaaaagcaa
     6421 aattctttac aggccgcgaa aaagcagctg ctcaattaac cgcgattaaa gaaaaagctc
     6481 gccgtacatt aggtgaggaa aaagaggcga ttttcgaagg ccacttgatg atcttagaag
     6541 atgaagagct tgaagaagaa attttgagtt accttactga taatcttgtg actgcggatg
     6601 ttgcagcaag taaagtgatt gagatgcagg cttcaatgct tgcggaaatt gacgatgaat
     6661 atttaaaaga acgtgcaggt gatattcgtg atattggtaa ccgcttggta cgcaatattt
     6721 taaatatgaa tatcgtagat cttggtgata ttcaagatga agttatttta gtcgcttatg
     6781 acttaacccc ttccgaaaca gctcaattaa atttagataa agtattaggc tttattaccg
     6841 atgtcggtgg tcgtacttct cacacttcta ttatggctcg ttccctagag cttcctgcca
     6901 ttgttggtac taacaatgtt acttccctag ttaaaactgg tgattttctg attttagatg
     6961 cagtgaacaa tgaagtacat ttaaatccat cggaagaaaa aatcgctaat ttcaaagcat
     7021 tacaagagaa attaaatgcg gaaaaagaag aattagccaa attaaaagag ttgcctgctc
     7081 aaacattaga tggccataaa gttgatgtgg cagggaatat cggtactatc cgtgatgtgg
     7141 aaggtgtatt acgcaacggt ggtgaagcgg ttggtttata ccgtactgag tttttgttta
     7201 tggatcgtac tcaattaccg ggagaagaag agcaatttca agcctacaaa gagattgttg
     7261 aagcaatgaa tggtcaaatg gtcattctgc gtacgatgga tatcggaggt gataaagagt
     7321 taccatacct tgatttaccg aaagaaatga acccattctt aggttggcgc gcggtgcgta
     7381 tcggtttcac ccgccgtgaa attttagata ctcagttacg tgcggtatta cgtgcttctg
     7441 cgttcggtaa tttagcggta atgttcccga tgattatttc ggtggaagaa atccgtttat
     7501 taaaatccat cgttgcggaa ttgaaagatc aattacgtgc agaaggtcac gcttttgatg
     7561 agaacttaaa actaggtatt atggtggaaa ccccatcagc agcggtaaat gcacgtcatc
     7621 ttgcaaaaga agccgacttc tttagtattg gtactaatga cttaacccaa tatacacttg
     7681 cggtagatcg tggtaatgaa attattgctc atttatataa cccattaagc ccatcagtct
     7741 taaacttaat caagcaagtt attgatgctt cacacgctga aggcaaatgg acaggtatgt
     7801 gtggtgaatt agccggtgac gtgcgtgcaa ctgcgttgtt gctcggtatg ggattagatg
     7861 aatttagtat gagcggtatc tctgtgccac acgttaaaaa actcattcgc tcaattaatt
     7921 ttgccgatgc taaagcatta gctgatgagg cgcttgctca gccaactgct gcagatattg
     7981 aacgcttagt agatgagttt tatcagaaat taaactaatt ttaattattt agcatacaaa
     8041 tacaaaattc tcgtataaaa tgtacgagaa ttttgtatct aatatctaag ggagatttta
     8101 agatgggttt atttgataaa ttattcggct caaaacaaaa agagagtcaa gaaatcaaaa
     8161 tctatgcgcc attaacaggt gaaatcgtaa atattgaaga tgtgcctgat gttgttttct
     8221 ctgaaaaaat tgtaggtgac ggtgtggcaa ttcgcccgag tggagacact atcgttgctc
     8281 cagtaaatgg gactatcggc aaaatttttg aaaccaacca cgctttttcg attgaatctg
     8341 aagaaggcgt ggaattattt gttcacttcg gtattgatac tgttgaatta aaaggcgaag
     8401 gctttactcg ccttgcacag gaaggtcaaa ccgtaaaagt gggcgagccg atcattaaat
     8461 ttgatttagc tctacttgaa ggcaaagcca aatcagtatt aacaccaatc gtgatttcaa
     8521 atatggatga aatcagcaac ttatctaaat taaatggcca agttgttgcc ggtgaaagcg
     8581 tggttttaac cttaaccaaa taatacctcc tacttagctg gaagccacga tggaagatcg
     8641 tggctttttt gttttaggt
//
LOCUS       DANLXF010000094         8636 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.094, whole genome shotgun sequence.
ACCESSION   DANLXF010000094 DANLXF010000000
VERSION     DANLXF010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8636
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.094"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..562
                     /locus_tag="PXD66_001423"
     CDS             <1..562
                     /locus_tag="PXD66_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDL6319717.1"
                     /translation="VQYPEEIDIKSKNGKVAKQFCQRKNLPLYITEISKERIRRAGKQ
                     ILQNNPENRPLDVGFKVFKLTESHFKQWQSPTSENLADQLEMFIDPVSEKADTQAVLY
                     ELLLRLGLKLTAKVRLENKVFWVEENGLIFALLLNAADEEIIQTVIAQQPKKVIALDR
                     LFNGNDARKKNTELQMQDAGITFFVI"
     gene            572..3208
                     /locus_tag="PXD66_001424"
     CDS             572..3208
                     /locus_tag="PXD66_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL6319718.1"
                     /translation="MELQFETLEYQLQAVNATVNLFVGQPNAATEFSLKAQNDMRFVP
                     NLGLQISDEQLQQNLANLQNRQKIDRTLLVEQGKNFTVEMETGTGKTYVYLRTIFELN
                     RQYGWQKFVIVVPSVAIREGVLHTLETTKSHFNTVFDNPSVNQKFEYKSNQTSRLKSF
                     ASANHIEILVMNIDAFTKESNVINTVNESGDAPIFYIQQANPIVIIDEPQNMETEIRR
                     NAIESLSPLFTLRYSATHKNAYNPIFSLNPVQAYEMGLVKQIEVDSVLAENEVNGAYL
                     SLKEIKTGAKSWSAKIEILFNDKKAMKKKVISVKPNQDLYELSNQNEMYRSGFILSGM
                     DTDSQMVSFSNGTQIFQGAEHSQLKEDVQKMQIRRTIGEHLQKEKKLRPLGIKVLSLF
                     FIDKVDNYRPSAQSPTGGKFAKWFEEIYLELAKEEPQGVHNGYFSQDKKGVAKDTNGT
                     TQADNETYQLIMRDKEKLLSLENPLRFIFSHSALKEGWDNPNVFQICTLNDTASEMKK
                     RQEIGRGLRLPVNQQGLRIYDRHLNTLTIIANESYEQFAATLQREIEEDCGVQFAKSN
                     VKKREDKKAVRYRKDFSLHPEFAAIWERIKHQTTYRVDFSNAELIESAVEKIRKMPDI
                     EATKIKHSKVKLEMNETGISTNYRSSNETAVNTQWAIPDLLSEIQKKTGLTRATLCKI
                     LQKSDRLQEVFRNPQRFIDLIAEKINLALQQIMTDGVEYHKIAGKTYEMTLFGDFDFY
                     LNQYTFEVQDKTKTIYEDYIALDSQTENIFARHCESSEQVEFYFKLPKRFKIPTPLGS
                     YNPDWAVVFKGENKIYLVAETKNTGKGIQAGVDEEKLDESERMKIDYARKHFKGLQRD
                     YKDLDYRVVQKVEELSAEYCKI"
     gene            3340..5772
                     /gene="gyrB"
                     /locus_tag="PXD66_001425"
     CDS             3340..5772
                     /gene="gyrB"
                     /locus_tag="PXD66_001425"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDL6319719.1"
                     /translation="MSEQTQATSYDSSSIKVLRGLDAVRKRPGMYIGDTDDGTGLHHM
                     VFEVVDNAIDEALAGHCKDIIVTIHSDNSVSVQDDGRGIPVGIHPEEGVSAAEVIMTV
                     LHAGGKFDDNSYKVSGGLHGVGVSVVNALSSKLQLTIRREGHVHEQFYSLGEPDAPLA
                     IIGNTEKTGTSVRFWPSLDIFKNKTEFEYKILSKRLRELSFLNSGVSIKLIDERDGKE
                     EHFKYEGGIKAYVEYLNEGKTHIHNTPFYLTTEKDGIGVEISLQWNDSYNENVYCFTN
                     NIPQRDGGTHLAGFRGALTRALKNYMDNSGVLKKADANIDASGDDAREGLVAVISVKV
                     PDPKFSSQTKDKLVSSEVRGAVESSMNEALSDYLAENPDDAKNIVTKIIDAARAREAA
                     RKAREMTRRKGALDLGGLPGKLADCQEKDPALSELYLVEGDSAGGSAKQGRDRKNQAI
                     LPLKGKILNVEKARFDKMLSSQEVATLITALGTGIGRDDYNLEKLRYHKIIIMTDADV
                     DGAHIRTLLLTFFYRQMPELIENGYIYIAQPPLYKVKKGKQERYIQDNDEMSQYEIDI
                     ALEGAGLYVSEDAPALSGVALENLIAQYNGVQKLIERLSRRYPTLLLNELIYSKPLSV
                     EFAKNQANMTAWSEQFVATLMAKEVGGSFYRINTLFNEERQIYEPEIIVTTHGMDSTY
                     RLDYNFINGNEYARIVALGNELNDLLSDSAYVVRGERRQEISSFAEALDWLVKESRKG
                     LTIQRYKGLGEMNPEQLWETTMDPVARKMLQVNITDAIAADKLFSTLMGDEVEPRRDF
                     IETNALYAQLDI"
     gene            complement(5825..6787)
                     /gene="cmoB"
                     /locus_tag="PXD66_001426"
     CDS             complement(5825..6787)
                     /gene="cmoB"
                     /locus_tag="PXD66_001426"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005707797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HDL6319720.1"
                     /translation="MIDFRPFYQQIATTDLSAWLETLPLQLKQWEKTTHADYAKWAKV
                     ADFLPNSTACINLKDKVESISSKPLSEGEVKQITHHLKQLMPWRKGPYHLHGIHIDTE
                     WRSDFKWDRVLPHLAPLKDRTILDVGCGSGYHMWRMVGEGAKMVVGIDPTELFLCQFE
                     AVRKLLGNDRRANLIPLGIEQMQPLAAFDTVFSMGVLYHRKSPLDHLSQLKAQLVRGG
                     ELVLETLVIDGDINDVLVPADRYAKMKNIYFIPSVAALINWLEKVGFKNVRCVDEAIT
                     TLEEQRKTDWLENESLVDFLDPNDHSKTIEGYPAPKRAVILANA"
     gene            complement(6884..>8636)
                     /locus_tag="PXD66_001427"
     CDS             complement(6884..>8636)
                     /locus_tag="PXD66_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HDL6319721.1"
                     /translation="TFITGKDAALEDSIATFQHKLKTLGFNIEEASWLNPVPNVWSVH
                     IRDADCPQCFSNGKGASKKAALASALGEYFERLSTNYFWADFYLGQEIANSEFVHYPT
                     EKWFPIDDEAIPPMEMMDEFLWEYFDPNGEITPELLVDLQSGNYDRGIVTLPYVRQSD
                     NETVYIPQSIIGNLFVSNGMSAGNTKNEARVQGLSEVFERFVKNRIITEAISLPEIPQ
                     SVIDGYPTIKASIEKLEQEDFPILCYDASLGGEFPVICVILLNPNNGTCFASFGAHPN
                     FQVAFERTVTELLQGRSLKDLDVFAPPSFNNEDVADHANLETHFIDSSGLISWDLFKR
                     DADYPFVHWDFSGTTEQEFNNMMAIFNKHNQPVYIMDYEHLGVYACRILAPGMSNIYP
                     SDDLVYANNNMGMDWREILLDLPHFHHDAETYQELLDELDEQSIDDLTRVREFIGVVA
                     EKGSAMQTLRIGELKSMLHLALGNLEQASDWANWTANMNASVFSAERNNYYRCLIQSI
                     ELFLDEERDSEQYRMVFEKMYGKQAVEAAWGAIQGGNPFHGLTASDESLENMTAHQKL
                     LNAYRKVQKAKVNPIAR"
BASE COUNT         2731 a         1679 c         1854 g         2372 t
ORIGIN      
        1 ggttcaatat cctgaagaaa ttgatatcaa gagtaagaat ggaaaagtag caaagcaatt
       61 ttgccaaaga aaaaatttac cactatatat aactgaaatt tcaaaagaac gtattcgccg
      121 tgccggaaag cagattttgc aaaataaccc cgaaaaccga ccgcttgatg taggttttaa
      181 agtcttcaaa ctgacagaaa gccattttaa acaatggcaa tcgcccacat ctgaaaattt
      241 agccgatcaa cttgaaatgt ttattgatcc tgttagtgaa aaagcggata cgcaagccgt
      301 tttatatgag cttttattgc gtttagggtt aaaactcacg gcaaaagtgc ggttggaaaa
      361 taaggtgttt tgggtggaag aaaacgggct gattttcgcc ttgctgttaa atgcagccga
      421 cgaagaaatt attcaaactg ttatcgctca acaaccgaaa aaagtcatcg ctttagaccg
      481 tttattcaac ggcaatgatg cccggaagaa aaacaccgaa ttgcaaatgc aagatgccgg
      541 cattacgttt tttgtgattt aaggagaaaa aatggaactc caatttgaaa ccttagagta
      601 tcaattacaa gccgttaatg ccacagtaaa tttgtttgtc ggacagccga atgccgcaac
      661 tgaattttca ctcaaagccc aaaatgatat gcggtttgtg ccgaatttag gcttgcagat
      721 tagtgatgag cagttacaac agaatttggc taatctgcaa aacaggcaaa aaatcgaccg
      781 cactttgctt gtcgaacagg gaaaaaattt taccgtggaa atggaaactg gtacagggaa
      841 aacctatgtc tatttacgca cgatttttga attaaaccgt caatacggct ggcaaaaatt
      901 tgtgattgtc gtgccgagtg tggcaattcg tgagggagtg ttgcatacgt tggaaactac
      961 aaaaagccat tttaatacgg tatttgataa cccgagcgtc aatcaaaaat ttgaatataa
     1021 aagtaaccaa acttcacgct taaaatcctt tgctagtgct aatcatattg aaattttagt
     1081 gatgaatatt gatgctttca ccaaagaaag taacgtgatt aataccgtga atgaaagcgg
     1141 tgatgctccg attttctata ttcaacaggc gaatccgatt gtgattattg atgaaccgca
     1201 aaatatggaa acggagattc gccgtaatgc gattgagagt ttaagtccgc ttttcacttt
     1261 gcgttattct gccacccata aaaatgcgta taacccgatt tttagcctca atccggtgca
     1321 agcctatgaa atgggattag ttaaacaaat tgaggtagat agcgtattgg cggaaaatga
     1381 ggtaaatgga gcttatcttt ccctcaaaga gattaaaaca ggggcaaaaa gctggtcggc
     1441 aaaaatcgaa attttattta acgataaaaa agcgatgaaa aagaaagtga ttagcgtgaa
     1501 accgaatcag gatttgtatg agctttccaa ccaaaatgaa atgtatcgtt cagggtttat
     1561 attaagcgga atggataccg atagccaaat ggttagcttc tccaacggca cacaaatttt
     1621 tcaaggggcg gaacattccc aacttaaaga agatgtgcaa aaaatgcaga ttcgccgcac
     1681 tattggcgag catttgcaaa aagaaaagaa attacgaccg cttgggatca aggtgttgtc
     1741 tttgttcttt atcgataaag tggataatta tcgcccttct gcacaaagcc ctacgggggg
     1801 aaaatttgca aaatggtttg aggaaattta tttggagcta gccaaagaag agcctcaagg
     1861 cgtgcataac ggttatttct cacaggataa aaaaggcgta gcaaaagata ctaatggcac
     1921 aacgcaagcg gataacgaga cttatcaact gattatgcgg gataaagaaa aattgctttc
     1981 gctggagaat ccgttacggt ttattttctc gcattctgcc ttaaaagaag gatgggataa
     2041 tccgaatgtg tttcaaattt gtactttaaa cgatactgca agcgaaatga aaaaacgcca
     2101 agagattggg cggggcttgc gtttgcctgt gaatcaacaa ggattgcgga tttatgatcg
     2161 tcatctaaat acgctgacaa ttatcgcaaa tgaaagttac gaacaatttg ccgccacatt
     2221 acagcgtgaa attgaggaag attgtggtgt acagtttgcc aaaagcaatg ttaaaaaacg
     2281 ggaagataaa aaagccgttc gctaccgtaa agatttcagc ttacatccgg aatttgcggc
     2341 aatttgggaa cggattaaac accaaaccac ttatcgggta gatttcagta atgcggaatt
     2401 gattgaaagt gcggtggaaa aaatccgcaa aatgcccgat atcgaggcga caaaaattaa
     2461 gcattccaaa gtgaaattgg aaatgaatga aacagggatt tcaactaatt atcgctcatc
     2521 taatgaaaca gcggtaaata cacaatgggc gatacctgat ttattgagtg agattcaaaa
     2581 gaaaaccggg ctgacgcgtg cgaccctttg caaaatttta caaaaatccg accgcttgca
     2641 agaagtcttc cgcaatcctc aacgctttat tgatttaatt gcagagaaaa tcaaccttgc
     2701 tttacaacaa attatgacag acggggtgga atatcacaaa attgccggaa aaacctatga
     2761 aatgacgtta ttcggcgatt ttgattttta cctcaatcaa tatacttttg aagttcaaga
     2821 taaaacaaaa acgatttatg aagactatat tgcactggat tcccaaacag aaaatatctt
     2881 cgcccgacat tgtgaatcta gtgagcaagt ggagttttat tttaaattac ccaaacgttt
     2941 taaaattcca acgccattag gcagctataa tcccgattgg gcagttgtgt ttaagggaga
     3001 aaataaaatt tacttggttg ccgaaacgaa aaatacaggc aaaggtattc aagcaggagt
     3061 agatgaagaa aaattagatg aaagtgaacg aatgaaaatc gactatgccc gtaagcattt
     3121 caaaggctta cagagagatt ataaggattt ggattatagg gttgtacaga aagtggagga
     3181 gctgtctgcg gagtattgta aaatttaatc tgtggtcttg tagaattgag atagactgca
     3241 atttcaacta aaatttaacc gcttgttttg cgatactttt acgcattttt ctgctataat
     3301 cggcacaatt ttttcgattt caaattagta ggaataagaa tgtctgaaca aactcaagca
     3361 acaagttatg attcttccag tattaaggtg ttacgcggct tagatgcggt gcgtaaacgc
     3421 ccgggaatgt atatcggcga taccgatgac ggtacgggct tacaccatat ggtgtttgag
     3481 gtggttgata atgcgattga cgaagcatta gccgggcatt gtaaagacat tattgtcaca
     3541 attcattccg ataattcggt ttctgtgcaa gatgacggtc gtggaattcc ggtggggatt
     3601 cacccggaag aaggcgtttc ggcagcagaa gtcattatga ctgttcttca cgcaggcggt
     3661 aaatttgacg ataactccta taaggtttcc ggcggtttgc acggtgtggg cgtatcggtg
     3721 gtgaatgcac tttcctctaa attacaactc actattcgcc gtgaaggaca cgttcacgag
     3781 caattctata gcttaggtga gcctgatgca cctttagcga ttatcggcaa taccgaaaaa
     3841 acggggacgt cggttcgttt ctggccaagt ttggatattt tcaaaaataa aaccgaattt
     3901 gaatataaaa ttctgtctaa acgcttgcgt gaactatcat ttttgaattc aggcgtttcg
     3961 attaaattaa ttgatgaacg agacggcaaa gaagagcatt ttaaatatga gggcggtatt
     4021 aaagcctatg ttgagtattt aaacgaaggc aaaactcata ttcacaatac gccattctat
     4081 ttgaccactg agaaagacgg cattggcgtg gaaatttccc tacagtggaa tgacagttac
     4141 aacgaaaatg tgtactgctt taccaataat attccacagc gtgatggcgg tacgcactta
     4201 gccggtttcc gtggggcatt aactcgtgcg ttgaaaaact atatggataa cagcggggta
     4261 ttgaaaaaag ccgatgcgaa cattgatgcc tcaggtgatg atgcccgtga aggtttagta
     4321 gcggtgattt cggtgaaagt gccggatccg aaattctcct cacaaaccaa agataaattg
     4381 gtgtcgtcag aagtgcgtgg agcagtggaa agctcaatga atgaggcttt atccgactat
     4441 ttagccgaaa atccggacga tgcaaaaaat atcgttacca aaattattga tgcagcccgt
     4501 gcaagagaag cagctcgtaa agcccgtgaa atgacccgcc gtaaaggggc gttagattta
     4561 ggcggcttac cgggtaaatt agctgattgt caggaaaaag atccggcgtt atcggagctt
     4621 tacttggtgg agggtgactc tgccggtggc tcggcaaaac aagggcgtga ccgtaaaaac
     4681 caagcgattt tgccgttaaa agggaaaatt ctgaacgtgg aaaaagcccg ctttgataaa
     4741 atgctttctt cacaagaagt agcaaccttg attaccgctt taggcacagg catcgggcgt
     4801 gatgattata atttggaaaa attacgttac cacaaaatca ttattatgac cgatgcggac
     4861 gtggatggtg cacatattcg tacgctttta ttaactttct tctaccgtca aatgccggag
     4921 ctgattgaaa acggctatat ttatattgca cagccaccgc tttataaagt gaaaaaaggc
     4981 aagcaagagc gttatattca agataacgat gaaatgtcgc aatatgaaat tgatattgca
     5041 ttagaaggtg caggcttata tgtgagtgaa gatgctccag cgttaagtgg cgtagcgtta
     5101 gagaacttaa ttgcccaata taacggcgta caaaaattaa ttgaacgttt aagccgtcgt
     5161 tacccaacat tattacttaa cgaattaatt tattcaaaac cgctttcggt ggaatttgca
     5221 aaaaatcaag caaatatgac cgcttggagc gagcaatttg tcgctacatt gatggcaaaa
     5281 gaggtgggcg gcagtttcta ccgtattaat accttgttca atgaagaacg ccaaatttat
     5341 gagccggaaa ttattgtgac tacacacggt atggacagta cctatcgttt agattacaac
     5401 tttatcaacg gtaatgaata cgctcgcatt gtggcattag gcaacgagct gaacgattta
     5461 ctatctgatt cagcctatgt ggtgcgtggt gaacgtcgcc aagaaatcag cagttttgct
     5521 gaagccttag attggttggt gaaagaatct cgtaaaggct taactattca acgttataaa
     5581 gggttaggtg agatgaatcc ggaacaatta tgggaaacca cgatggatcc ggtggctcgt
     5641 aaaatgctac aggtcaatat taccgatgcg attgcggcag ataaactctt cagtacgctg
     5701 atgggcgatg aagttgaacc acgccgagat ttcattgaaa ctaacgcttt gtatgctcag
     5761 ttagatattt agttaaaaaa tccgcatttg acatgcggat tttttattct cttaaactag
     5821 cattttaagc attcgctaaa atcaccgccc gcttcggagc agggtagcct tcaatggttt
     5881 tgctatgatc gtttggatct aaaaaatcca ctaaactttc attttccaac caatcggttt
     5941 ttcgctgctc ttctagtgta gtgattgcct catcaacaca acgcacattt ttaaagccga
     6001 ctttttccag ccaattaatg agtgcagcaa cagaaggaat aaaatagata tttttcattt
     6061 tcgcatagcg atcagccggc actaaaacat cattaatatc gccatcaatc accagcgttt
     6121 ctaacaccaa ttcaccgcct ctgactaatt gggctttgag ttgagaaaga tgatcaagcg
     6181 gtgatttgcg atgataaagc acccccattg aaaacacggt atcaaaagca gcaagcggtt
     6241 gcatttgctc aataccaagt ggaatcagat tagcccgtct atcattccct aagagtttcc
     6301 gcactgcctc aaattggcac aggaacagct cggtcgggtc aataccaact accatttttg
     6361 ccccttcgcc taccattcgc cacatatgat aaccgctacc gcagccgaca tctaaaatgg
     6421 tgcggtcttt taacggtgca agatgcggaa gcacacgatc ccatttaaaa tcggaacgcc
     6481 attcggtatc aatatgaatc ccgtgtagat gataagggcc tttacgccac ggcattagtt
     6541 gctttaaatg gtgggtaatt tgcttgactt ccccctcaga taaaggcttg cttgaaatag
     6601 actcaacctt gtcttttaga ttgatacaag cggtcgaatt tggtaaaaaa tctgcaactt
     6661 ttgcccactt ggcatagtca gcgtgggtgg tcttttccca ttgtttgagt tgtaagggta
     6721 aggtttctaa ccaagcagaa aggtcggttg tggcaatttg ttgataaaaa ggacgaaagt
     6781 caatcatttt gaaatttatg tctaggggag agacctatgc cctcccgaaa ggtagtaaat
     6841 agaaaggaca ggcacagaac ctacccctac gaaaaatata aaattatctc gcaatcggat
     6901 ttactttcgc tttttgcact ttgcgataag cgttcagtaa tttctgatgt gcagtcatat
     6961 tttcaagcga ttcgtccgat gcagttaaac cgtggaacgg gttaccgcct tggattgcac
     7021 cccaagcagc ttcaaccgct tgtttaccgt acattttctc aaacaccata cgatattgtt
     7081 ccgaatcacg ctcttcatct aagaacaact caatcgattg gattaagcag cggtaataat
     7141 tgttacgttc tgctgagaat acgcttgcat tcatattcgc tgtccagttt gcccagtctg
     7201 aggcttgctc taagttacct aaagctaaat gcagcatcga tttaagctca ccgatacgca
     7261 atgtttgcat tgcagagcct ttctctgcca ccacgccaat aaattcacgc acacgggtta
     7321 aatcatcaat gctttgctca tctaattcat caagcagttc ttggtaagtt tcagcatcgt
     7381 gatggaaatg tggcaaatct aacaagattt cacgccaatc catacccata ttgttattgg
     7441 catagaccaa atcatcgctt ggatagatat tcgacatacc cggtgcaaga atacggcaag
     7501 cgtacacacc taaatgctca taatccataa tatacacagg ctgattatgc ttgttgaaaa
     7561 tcgccatcat attattaaat tcttgctccg ttgtgccgga gaagtcccaa tgcacgaacg
     7621 gataatccgc atcacgtttg aaaagatccc acgaaattag accgcttgaa tcaataaagt
     7681 gtgtctcaag gttcgcatga tctgctacat cttcattatt aaatgaaggt ggggcaaaca
     7741 catctaaatc ctttaagcta cggccttgta ataactcggt tacggtacgt tcaaacgcca
     7801 cttggaaatt cgggtgtgca ccaaaagagg caaaacaagt accgttattt gggtttaaca
     7861 agatcacaca aataactgga aattctccgc ctaatgaagc gtcatagcat aaaatcggga
     7921 agtcttcttg ctctagtttc tcgatagagg ctttgattgt cggataaccg tcaatcacac
     7981 tttgcggaat ttccggcaag ctgattgcct cagtaataat acggtttttg acaaaacgct
     8041 cgaacacttc ggataagccc tgcactcgag cttcattttt tgtgttgcct gccgacatac
     8101 cgtttgaaac aaacaggttg ccgataatgc tttgcggaat gtaaaccgtt tcattgtcag
     8161 attggcgaac gtaaggtagg gtaacaattc cacgatcata atttcctgat tgtaaatcaa
     8221 ccaataattc cggcgtgatt tcaccattcg gatcaaaata ttcccataaa aactcgtcca
     8281 tcatctccat tggtggaatg gcttcatcat caatcggaaa ccatttttcg gttggataat
     8341 gcacaaattc actattagca atctcttgac ctaaataaaa atccgcccag aaatagttgg
     8401 tagataaacg ctcaaaatac tcacctaatg cagaggcaag agcggctttt ttgcttgctc
     8461 ccttaccgtt agaaaaacat tgcggacagt ctgcatcacg aatatgcacc gaccacacat
     8521 tcggaacagg atttaaccaa gaggcttctt caatattaaa acctaaggtt tttaatttat
     8581 gctgaaaggt cgcaatgctg tcttcaagtg cagcatcttt accggtaata aaagtt
//
LOCUS       DANLXF010000095         8587 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.095, whole genome shotgun sequence.
ACCESSION   DANLXF010000095 DANLXF010000000
VERSION     DANLXF010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8587
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.095"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(407..2524)
                     /gene="spoT"
                     /locus_tag="PXD66_001428"
     CDS             complement(407..2524)
                     /gene="spoT"
                     /locus_tag="PXD66_001428"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HDL6319722.1"
                     /translation="MYLFEPLDRIIQGYLPPEQIERVKRAYVIARDAHEGQTRSSGEP
                     YITHPVAVASIIAEMKLDQEAVIAALLHDVIEDTPYTEEELAAEFGQSVADIVQGVSK
                     LDKLKFRTRQEAEVANFRKMILAMTKDIRVVLIKLADRTHNMRTLGALRPDKRRRIAK
                     ETLEIYSPLAHRLGIEHIKNELEDLGFEAMHPQRYRVLKMAIASARGHRKELIQRIAD
                     DIRGRLEEVQIEARVYGREKHLYSIYQKMRQKDQHFHSILDIYAFRVVVDNVDNCYRV
                     LGQMHALYKPRPKRVKDYIAVPKPNGYQSLHTSMIGPHGVPVEVQIRTEDMDKMADMG
                     VTAHWRYKEGESHHNTTVQLKAQQWLQSIVELQNSAGNTAEFIDSVKSDLFSDDIFVF
                     TPKGRIVELPANATAIDFAYAVHSGIGDRCVGAMVDRNPYPLSEPLRTGQTVEIITEQ
                     GKRPNASWLNFVVSGKARAKIRQSLKNQQHDEAIALGRRQLVRAMNLRSLDELCSETI
                     QSVLDELRLSSFDELLAEIGLGNQMSNVIAQRLLGEPLLIDTDGNPENNQPLKIEGIE
                     NRLISFANCCLPIPGDEIIAHVSSGKGLAIHNACCGNVKDYATEPSNYLPVEWEIAKE
                     KAIQFETEIVLDIINEPGVLAGITSTLAKLNSNIGSINSEAREGNIYQVRVQISVENK
                     EHSELILRKLLSVKGVVKAARKQ"
     gene            complement(2634..2915)
                     /gene="rpoZ"
                     /locus_tag="PXD66_001429"
     CDS             complement(2634..2915)
                     /gene="rpoZ"
                     /locus_tag="PXD66_001429"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL6319723.1"
                     /translation="MARVTVQEAAEKIGNRFDLILIAARRARQLQLHTREPLVPEEND
                     KPTVIALREIEEGLITTDIMDQLENHDSIKQEAAEQEAISFLTEMSNEA"
     gene            complement(3018..3515)
                     /locus_tag="PXD66_001430"
     CDS             complement(3018..3515)
                     /locus_tag="PXD66_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL6319724.1"
                     /translation="MAHFYEYLEFNSDEDRENQLEVYVEVKLSTDTEEKLQAVGVQGD
                     WLVMAEPYCPDCVEIVAYFQRMAKLNPNIKVKYVARKDNQERKHFDSDEQQQAVISAQ
                     KIPTIFDIRNGKTELVLSEFPQFLKDKMAANPEQFEQLKADFRMGKFGKEVEAELLAI
                     LLNTK"
     gene            complement(3540..4562)
                     /locus_tag="PXD66_001431"
     CDS             complement(3540..4562)
                     /locus_tag="PXD66_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005825060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL6319725.1"
                     /translation="MSVTKKRLTLSDIAELSGVSKTTVSMILNGRGNDFRIKSETCQK
                     VKEIAKKHGYRANVYAKSLQAQRTNVIGLVIPDLTNYGFALTSRTLEKLCRENGLQLV
                     IACSDDTFEGEKKAIERLLDRQVDLLITAPTHNEPTYYQNAAQYTPMLQLDRYISGLN
                     IPSITSNDTQSISMLVENIVRAYHLKEFFYLGGQLSLSPSQNRLKGFREGLEQSHLAL
                     DENWVLHQDYQPESGYQMFAQIVEKLGRLPEAVFTASFTLLEGVMRYLTEHQQMDKLL
                     TQELHLATFDDHHLLNALPFHIHSIKQDHQQIAERTFELIQQKLAQQKIDDVKVDCEI
                     LWRKSI"
     gene            complement(4602..6032)
                     /locus_tag="PXD66_001432"
     CDS             complement(4602..6032)
                     /locus_tag="PXD66_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HDL6319726.1"
                     /translation="MHIFNNGKYKSLHAQSEGELNALRQTVLSDPDFYPTYHLAPETG
                     LFNDPNGLIFDGEKYHIFAQWFPYGALHGMKHWQHFITTDFQTFSKGDLLIPDELFES
                     HGCYSGGAILWQDKIVAFYTGNTRRPSDNARIPHQNIAIFDKSGKLLEKRCIIDQAPA
                     GYTEHVRDPKPYLTAEGKIRFVLGAQRENLTGTALIYEMDDLNATPRLIAELEVHNFD
                     NSNVFMWECPDLFSLGGKDLFVWSPQGKLRESHQFQNNYHATYALGELNGNRLRAEHI
                     EELDYGFDFYAPQTVENSDRILFGWVGLPDLTYPTDKYQWHSMLTLPRKLSLQNGKVI
                     QNPIVNVGELQAVEMSENFAIENLDTAYLQISVENQPLVLDFFSNEQGQKLSLRFENG
                     VLSLDRSQTEQTELMEKFGSVRHCKIEQLETIEIFFDRSVVEIFVNGGEKVLTSRFFI
                     AKRENVVKSSRPIQVKVAKVLPISVE"
     gene            complement(6137..7060)
                     /locus_tag="PXD66_001433"
     CDS             complement(6137..7060)
                     /locus_tag="PXD66_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HDL6319727.1"
                     /translation="MSKIWVTGDAVVDLIPDGEHHYLKCAGGAPANVAVGVSRLGVEA
                     GFIGRVGNDPLGKFMREVLQAENVCTKQMILDAQHRTSTVIVGLDNGERSFTFMVNPS
                     ADQFLEIGDLPEFNQGDFLHCCSIALINDPSRSTTIEGIRRVKKAGGYVSFDPNLRES
                     LWKSLDEMKTVVNSVVAMADILKFSEEELTLLTETETLEQATQAITAQYPEKLIIITL
                     GKDGAIYHFNGKSQVVAGKALKPVDTTGAGDAFVSGLLAGLAQTPNWHDETVLVEVIR
                     KANASGALATTAKGAMSALPNKAELDAFLAE"
     gene            complement(7177..8568)
                     /gene="miaB"
                     /locus_tag="PXD66_001434"
     CDS             complement(7177..8568)
                     /gene="miaB"
                     /locus_tag="PXD66_001434"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014390822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDL6319728.1"
                     /translation="MNEYDSSKMADLLNSTHGFELTENPEDADVLLLNTCSIREKAQE
                     KVFSQLGRWKNWKKDKPNLIIGVGGCVASQEGEHIRDRAPYVDIVFGPQTLHRLPEMI
                     NQIRGGKSSIVDISFPEIEKFDRLPEPKAEGPTAFVSIMEGCNKYCSFCVVPYTRGEE
                     VSRPVDDVLFEIAQLAAQGVREVNLLGQNVNAYRGETFDGGICTFAELLRLVAAIDGI
                     DRLRYTTSHPIEFTDDIIEVYRDTPELVSFLHLPIQSGSDRVLTMMKRNHTALEYKAI
                     IRKLRAVRPEIQISSDFIVGFPGETAEDFEQTMKVIEQVNFDMSFSFIYSARPGTPAA
                     DLPDNVSDDEKKERLSRLQQRINHQAMQFSRAMLGTEQRILVEGPSKKDLMELTGRTE
                     TNRVVNFVGTPDMIGKFVDVKITDVYTNSLRGEVIRTEDQMGLRVVESAESVIARTRK
                     EDDLGVGKYVVNL"
BASE COUNT         2332 a         1972 c         1687 g         2596 t
ORIGIN      
        1 tacattttcg agaaaatttt cgctaagccc accatcttga ttaggctttc tagatatatg
       61 ccaaaatcca gataaacgat aataccctac ctgagataat tttttctctg catagctttt
      121 atcatttatg agcattcctc tagatatgag tagttctact aattcgtgat actctttatg
      181 tggttttgta ggcataaaat tgaaggcatt ttaagaaagg caaggacgta gcttgacact
      241 ctaagtcgat tacatttagt aaacctcagg gacaggggct acgccacgat tgcaagggat
      301 tatacatttt agctgattaa agtcaaaagg tttcatcaat taataatagc aatactgtac
      361 ttttaaggac tataattgca aaaaactgat aaaattttac cgcttgttac tgcttacggg
      421 ccgcttttac aacacctttt acacttaata gcttacggag aatcagctca gaatgctctt
      481 tattttccac agaaatttgt actctcactt gatagatatt tccttctctt gcctcgctat
      541 taatgctgcc aatgttgcta tttaatttgg caagcgttga tgtgatcccc gcgagtaccc
      601 ctggttcatt gataatatcc agtacgattt ccgtttcaaa ttgaatcgct ttttctttgg
      661 caatttccca ttccacaggt aaataattac tcggctctgt tgcgtaatct ttcacattac
      721 cacaacaagc attgtggatt gccaatcctt tacctgaact gacgtgagca ataatttcat
      781 cacccggtat aggtaagcaa cagtttgcaa aactgataag gcggttctca atcccttcaa
      841 ttttaagcgg ttgattattt tctggattac catcagtatc aattaatagt ggctccccta
      901 ataagcgttg agcgataaca ttactcattt ggttacctag accaatttca gccagtagct
      961 catcaaagct tgataaacgt agctcgtcta atacggattg aatagtttct gaacacaatt
     1021 catcaagtga gcgtaaattc attgcccgaa ctagctgtct acgcccaagt gcaatcgcct
     1081 catcgtgctg ctgattttta agcgattgac gaattttagc cctcgcctta ccacttacta
     1141 caaaatttaa ccacgatgca tttgggcgct taccttgctc ggtaataatt tccaccgtct
     1201 gtcctgttcg tagcggttct gaaagtggat acggattgcg atcaaccatc gcacctacac
     1261 aacgatcccc aatgcctgaa tgcaccgcat aggcaaagtc aatagcggta gcattcgccg
     1321 gcaattcaac aatacgccct ttcggggtaa agacgaaaat atcgtcagag aacagatccg
     1381 atttaacgct atcaataaat tctgctgtat tgccggcact gttttgcaat tcaacaatac
     1441 tttgtagcca ttgctgggct ttgagttgaa cggtcgtgtt atggtgcgac tcgccctctt
     1501 tgtaacgcca gtgagctgtt acccccatat ccgccatttt gtccatatct tcggtgcgaa
     1561 tctgtacctc caccggtaca ccgtgcgggc caatcatcga agtgtgtaaa gattgatagc
     1621 cgttcggttt tggcacggca atataatctt tcacccgttt cggacgaggc ttatacagtg
     1681 cgtgcatctg ccctaataca cggtaacaat tatctacatt atcaaccact actcggaaag
     1741 cataaatatc taaaatcgaa tgaaaatgtt gatctttctg gcgcattttt tggtaaatgg
     1801 aatagagatg tttttcacgc ccatacacac gggcttcaat ttgcacctct tctaaccgac
     1861 cacgaatatc atcggcaatg cgttgaatta gctctttacg atgtcctctg gcactggcaa
     1921 tagccatttt cagtacccga taacgttgcg ggtgcattgc ctcgaagcct aaatcttcca
     1981 actcgttttt aatatgctct attcccaagc gatgtgccaa cgggctataa atctctaagg
     2041 tttctttcgc tatacggcgg cgtttatccg gacgcaaagc accaagcgta cgcatattat
     2101 gggttcggtc ggcaagttta attaacacaa cccgaatgtc tttggtcatt gccaaaatca
     2161 ttttacggaa gtttgccact tctgcctctt ggcgggtgcg gaattttaat ttatccagct
     2221 tagaaacgcc ttgtacaata tctgctacgc tctgtccaaa ttccgccgcc agttcttctt
     2281 cggtgtaagg tgtatcttca atcacatcgt gcaacaaagc cgcaatcacc gcttcttgat
     2341 cgagcttcat ttccgcaata atcgacgcca ctgctaccgg gtgagtaata tagggctcac
     2401 cgctagagcg tgtttgtccc tcgtgagcat cacgggctat cacataagca cgttttacac
     2461 gctcaatctg ctcgggtggc aaatagcctt gaataattcg atctaaaggt tcaaaaagat
     2521 acacgttata cctcaacaac agaaataaaa atggacaacg aatttgcaaa ttttgaggaa
     2581 aatcctaccg cttgcagact caattgtcca ttctctattt acgaaaaata aaattacgct
     2641 tcgttgctca tttcagttaa aaatgaaatc gcctcttgct ccgccgcttc ttgcttaata
     2701 gagtcgtgat tttccagctg atccataatg tcagttgtaa ttaaaccttc ttcaatttca
     2761 cgtaatgcaa tcacggtcgg cttatcgttt tcttcaggca caagtggctc acgggtgtga
     2821 agttgtaatt gtcttgcacg acgtgcagca attaagatta aatcaaaacg gttgccaatt
     2881 ttctccgctg cttcttgtac ggttacacga gccataaatt tactccaaag atttattcgg
     2941 ttttattcat aaaaagattg cgaattatac agaaattccg ccaattccgc aatgaaagcg
     3001 ggctaaattc acaaaattta tttggtattc agtaaaatcg ccaataattc agcctcaacc
     3061 tctttgccga attttcccat tctaaaatcg gcttttagtt gctcgaattg ctcagggttc
     3121 gctgccattt tatcttttaa aaattgcgga aattcagata acactaattc tgttttgcca
     3181 ttacgaatat caaaaatagt tggaattttt tgcgcagaaa tgaccgcttg ttgctgttca
     3241 tcgctgtcaa aatgtttgcg ttcctgatta tctttgcgag ccacatattt caccttaatg
     3301 ttaggattga gctttgccat tcgctggaaa taagccacaa tttccacaca atcggggcaa
     3361 tatggctctg ccattaccag ccagtcccct tgcacgccaa cagcttgcag tttttcttcc
     3421 gtatctgtgg aaagcttgac ctccacatac acttctaatt gattttcacg atcttcatcg
     3481 ctgttaaatt ctaaatattc gtaaaaatgt gccataattt tgtcttttcc tatctcatct
     3541 taaatggatt tacgccataa aatttcacaa tcaactttaa cgtcatctat tttctgttgc
     3601 gctaactttt gctgaattaa ttcaaaggta cgttcggcaa tttgttggtg gtcttgctta
     3661 attgaatgaa tatgaaaagg tagtgcattc agtaaatggt gatcatcaaa ggttgctaaa
     3721 tgcagttctt gagtcaataa tttatccatt tgttggtgtt cagtcagata gcgcattact
     3781 ccctcaagta gggtaaaaga ggctgtgaat accgcttctg ggagcctgcc aagtttttca
     3841 actatctgag cgaacatttg atagcccgat tccggctgat agtcttgatg cagaacccaa
     3901 ttttcatcaa gtgccaaatg tgactgctcc aaaccttcac ggaagccttt taaacgattt
     3961 tgacttggcg agagtgaaag ttgtccacct aagtaaaaaa actcttttaa gtgataagcc
     4021 cgtacaatat tttccaccaa catactgata ctttgtgtat cattactggt gatagaaggg
     4081 atattcaacc cagaaatata gcggtctagc tgtaacattg gggtatattg tgccgcattt
     4141 tgataataag tcggctcatt atgtgtcgga gcagtaatca gtaaatctac ctgtctgtct
     4201 aataaacgct ctatcgcttt cttctcacct tcaaaggtat catccgagca agcgatcact
     4261 agctgtaacc cgttttcacg gcataatttc tctaaggttc ttgaggtcag agcaaagcca
     4321 taattcgtta aatcgggaat gactaaaccg atcacattcg tgcgttgagc ctgtaatgat
     4381 tttgcatata cattagcacg ataaccgtgt tttttagcga tctctttcac tttttggcag
     4441 gtttcggatt tgattctaaa atcattgccc cgcccattta aaatcatact gactgttgtt
     4501 ttagatacgc cgctgagttc cgcaatatca ctgagcgtta ggcgtttttt agtcacactt
     4561 atctcctttt agcataaaaa tgggcattac tgcccattgc tttattctac gctaattggc
     4621 aatacttttg ctactttcac ttgaatcgga cgagagcttt ttacgacatt ttcacgctta
     4681 gcgataaaaa agcgtgaagt cagcactttt tccccgccgt ttacgaaaat ttccaccaca
     4741 gaacggtcaa agaaaatctc gattgtctct aattgttcaa ttttgcaatg acgaaccgag
     4801 ccaaattttt ccatcaattc tgtttgttcg gtttggctac gatctaagct caacacccca
     4861 ttttcaaaac gtaagctgag cttttgcccc tgttcattgc tgaaaaaatc caacacaagc
     4921 ggttgatttt ccacagaaat ttgcaagtag gcagtatcca aattctcgat tgcaaaattt
     4981 tctgacattt cgaccgcttg taattcgcct acattcacga ttgggttttg tatcactttg
     5041 ccgttttgca gggaaagttt acgaggcaat gtcagcattg aatgccactg atatttatca
     5101 gttgggtaag tgagatcagg caagccgacc cagccaaaca aaatgcgatc ggaattttcc
     5161 accgtttgcg gtgcgtaaaa gtcaaagccg tagtccagct cttcaatatg ctctgcccgt
     5221 aatctgttgc cgtttagctc gccaagtgcg taagtggcgt ggtagttatt ttggaattga
     5281 tggctttcac gcagtttgcc ttgtggcgac cacacaaaca gatctttacc accaagtgag
     5341 aacagatccg gacactccca cataaacaca ttgctgttat caaaattgtg gacttccagt
     5401 tctgcgatta aacggggtgt ggcatttaaa tcgtccattt cataaattaa cgccgtgccg
     5461 gtcaagtttt cacgctgagc ccccaacaca aaacgaattt tgccctctgc ggtaaggtaa
     5521 ggtttcgggt cacgcacgtg ttcggtgtag cctgccggtg cttggtcgat aatgcaacgc
     5581 ttttccaata atttgcccga tttatcaaaa atcgcgatat tttggtgagg aatgcgggca
     5641 ttatctgatg ggcggcgtgt attgccggtg taaaaagcga caatcttatc ttgccataaa
     5701 atcgccccac ctgaataaca gccgtgggat tcaaacagct catcgggaat cagcaaatcc
     5761 cctttactga acgtttgaaa atcggtagtg atgaaatgct gccaatgttt cattccatgt
     5821 aatgccccat aaggaaacca ttgggcgaaa atatggtatt tttcaccatc gaaaatcaag
     5881 ccgtttgggt cattaaataa gcctgtttcg ggagcgagat gataggttgg gtaaaaatcg
     5941 ggatcagata aaacggtttg gcgaagagcg tttaattcgc cctcgctttg tgcgtgcagg
     6001 cttttgtatt tgccgttgtt gaaaatgtgc atagagtgtt cctgtcattt cagaaattac
     6061 gatggtgcaa gcgtccacgc ttgtaccctt ttgtaaaaaa tgaggcacaa gcgtggacac
     6121 ttgcgccatc attacattat tccgccaaaa atgcgtctaa ctccgcttta ttcggtaatg
     6181 ccgacatcgc cccttttgct gtggtggcta acgcaccgga agcatttgct ttgcggatca
     6241 cttcaactaa cacagtttca tcgtgccaat tcggcgtttg agcaagaccg gctaataatc
     6301 cgctgacaaa ggcatctccg gcaccggtgg tatccacagg tttcaaggct ttgccggcaa
     6361 cgacttggct ttttccgttg aaatggtaga ttgcaccgtc tttgccaagg gtgataataa
     6421 tcaatttctc cggatactga gcggtaatag cttgtgtggc ttgttccaaa gtttctgtct
     6481 cggttaataa ggtcagctct tcttcggaaa atttgagaat atccgccatc gccaccacag
     6541 aatttaccac tgttttcatt tcatcaaggc tcttccagag cgattcacgc aagtttggat
     6601 caaacgaaac atagccaccc gcctttttca ctcggcgaat cccttcaatg gtagtggaac
     6661 gggatgggtc gttaatcaag gcaatagaac agcagtgtaa gaaatcacct tggttaaatt
     6721 caggtaaatc gccgatttct aaaaattggt cggcactcgg atttaccata aaagtaaaac
     6781 tacgctcacc gttatctaag ccaacaatca cggtggaagt gcggtgctga gcgtctaaaa
     6841 tcatctgttt ggtgcaaaca ttttctgcct gtaacacttc tcgcataaat ttacccagag
     6901 ggtcgttacc cacacgcccg ataaatcctg cctcaacccc taaacgagaa acgcctactg
     6961 caacattcgc cggagcacca ccggcacatt ttaaatagtg atgttcgccg tcgggaatta
     7021 aatccaccac cgcatcgcct gttacccaaa tcttactcat ctcatacacc tttaattagt
     7081 cacaaaacac caagctaaaa cgatttagca tttattcgat tatatactaa aagaccgctt
     7141 gtacatacct acaagcggtc attttttctt taatttttac agattaacca cgtatttacc
     7201 tacacctaaa tcatcttctt tacgagtgcg ggcaatcacg ctttctgccg attccaccac
     7261 acgtaatccc atttggtctt cggtacgaat cacttcgcca cggagtgagt tggtataaac
     7321 atcggtgatt ttcacatcga caaatttgcc aatcatatcc ggtgtgccaa caaagtttac
     7381 tacacggtta gtttcggttc tgccggttaa ttccatcaag tctttcttag acgggccttc
     7441 aaccaaaatg cgttgttctg taccgagcat tgcacggcta aattgcattg cttggtggtt
     7501 gatgcgttgt tgtaaacggg ataatcgctc ttttttctca tcatcggaga cattatcagg
     7561 cagatctgcc gctggtgtac ccggtcgagc cgagtagata aagctaaagc tcatatcaaa
     7621 gttcacttgc tcgatcactt tcattgtttg ctcgaaatcc tctgcggttt cgcccgggaa
     7681 gcctacaata aagtccgaac taatttgaat ctcaggtcga acagcacgca atttacggat
     7741 aatcgctttg tattccaacg ctgtgtggtt acgcttcatc attgttaata cacggtctga
     7801 accactttga atcggtaaat gtaagaaact gaccaattca ggtgtatcac gataaacttc
     7861 gataatatca tcggtaaatt caatcgggtg gctggtggtg taacgtaagc ggtcaatacc
     7921 gtcaatcgct gccactaagc gtaataattc ggcaaaggta caaatgccgc catcaaaagt
     7981 ttcaccacgg taagcattta cgttttgacc gagtaaatta acctcacgca caccttgtgc
     8041 cgcaagttgg gcgatttcaa ataatacgtc atccaccggg cgggaaacct cttcgccacg
     8101 agtgtaaggc acaacgcaga acgaacaata tttattgcag ccttccataa ttgaaacgaa
     8161 tgctgtaggg ccttcggctt ttggctccgg caagcggtca aatttttcaa tttccgggaa
     8221 agaaatatcg acaatcgagc ttttaccgcc acggatttgg ttgatcattt caggcaaacg
     8281 gtgcaaagtt tgtggcccga aaacaatatc tacataaggg gcacgatcac ggatatgctc
     8341 cccttcttgt gaagccacac aaccgccaac accgataatc aggttcggtt tatctttttt
     8401 ccagtttttc caacgcccga gttgcgagaa tactttttct tgtgcttttt cacggataga
     8461 acaagtgttt agcagcaaca cgtctgcatc ttcagggttt tcagtcagtt caaaaccgtg
     8521 agtagagttt aggagatccg ccatttttga tgagtcgtac tcattcattt ggcagcccca
     8581 agtggta
//
LOCUS       DANLXF010000096         8560 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.096, whole genome shotgun sequence.
ACCESSION   DANLXF010000096 DANLXF010000000
VERSION     DANLXF010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8560
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.096"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..244)
                     /locus_tag="PXD66_001435"
     CDS             complement(<1..244)
                     /locus_tag="PXD66_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319729.1"
                     /translation="MITSNLERSFHGLNIAQHNYKIFLDESGGNIGKLKHTPNDKKGS
                     NFNRTISSHIGVPPVFVLGGIAQISNANLIAPDWRKL"
     gene            complement(271..945)
                     /gene="cas4"
                     /locus_tag="PXD66_001436"
     CDS             complement(271..945)
                     /gene="cas4"
                     /locus_tag="PXD66_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HDL6319730.1"
                     /translation="MGDLQNSAEILPLVQPKFGDDKRSISLSALQHYAFCPRQCALIH
                     NEQVWAENYLTAQGQALHERVDSGEPEVRKGIRFERTVHVAAEKLGISGILDLVERDL
                     KTGELKPVEYKRGKPKLDPIDEIQLCAQALCLEEMTEQTINEGALWYMQTRHRLPIIF
                     STELRTKTLATIAEVRSLLDSGTTPPPEYSKRCKACSLIDLCQPKLLERDRSKGYVVG
                     VFGSLL"
     gene            complement(945..1808)
                     /gene="cas7c"
                     /locus_tag="PXD66_001437"
     CDS             complement(945..1808)
                     /gene="cas7c"
                     /locus_tag="PXD66_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HDL6319731.1"
                     /translation="MSIQNRYEFVFFFDVTNGNPNGDPDAGNMPRLDPETSKGLVTDV
                     CLKRKIRNFIEMSYENEAGYEIYVKEKSVLNLQNKRAYEALGIESEAKKLPKEEAKAR
                     EITAWMCKNFFDIRTFGAVMTTEVNSGQVRGPVQLAFAQSIDPIIPLEVSITRMAVTN
                     EKDLEKERTMGRKYIVPYALYRVHGFISAKLAEKTGFSDEDVQKLWQALQLMFEHDRS
                     AARGEMAARKLIVFKHDSELGNQPAHKLFDSVKVERINGEKDTPAKDYDDYCISVQTE
                     GLTGVTVEELL"
     gene            complement(1818..3599)
                     /gene="cas8c"
                     /locus_tag="PXD66_001438"
     CDS             complement(1818..3599)
                     /gene="cas8c"
                     /locus_tag="PXD66_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HDL6319732.1"
                     /translation="MITALTRYYYRLAAQEDRLTGEAKVPSYGFSEEKIGWVLELDSK
                     GNLVNVISNLTEDKKPKPKLMSVPRPEKRTSGIKPNFLWDKTAYVLGIESNPDKATAK
                     DQPINFAEKTFEAFKQYHLDLLADSNDEGLMAIRNFLQQWQPTHFAESICPREILDAN
                     VVFKLVGTSGYIHQREAAQTLWTSLLINQSDNAEKGLCLISGKLSPIQRTHPPIKGVF
                     GGQSSGGSIISFNKESFTSFGKEQGSNAPVSEQAAFAYTTALNYLLRTEKHRLTIGDA
                     STVFWAEADNTTQAEAAEGFFAALMTPPDDEQENQKIFEELQKISKGRPLAEISPELS
                     ENTRFYVLGLAPNAARISVRFWLDTTFGQLAKNLSEHWQDLALEPCPWKTPPSIWRLL
                     LQTASLGKSENISPVLAGEMARAVISGHLYPMSLLSQLVTRIRADGDINGLRVALMKA
                     VLQRKFRKGFIKEGVPMSLNKESDNQAYLLGRLFAVLERIQSQALGELNAGIADRYYG
                     SASAVPFSVFPRLLSGAKHHLSRLRKDKGGMAVNLDKDLGEIIASLPENFPRHLSIDE
                     QGRFAIGYYHQKQSYFAKKETTESIEN"
     gene            complement(3621..4298)
                     /gene="cas5c"
                     /locus_tag="PXD66_001439"
     CDS             complement(3621..4298)
                     /gene="cas5c"
                     /locus_tag="PXD66_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5"
                     /protein_id="HDL6319733.1"
                     /translation="MPNKIRLHIWGDYACFTRPEMKVERVSYDVITPSAARGILAAVH
                     WKPAIRWVIDKIYVLQPIQFESVRRNELGSKISASKISGAMKRKTVADLYTVIEDDRQ
                     QRAATVLKNVGYVIEAHAVLTKMAGEAETVTKHIEMFKRRAKKGQCFQQPCMGVREFA
                     ANFALIDDDEPLPECFLAENERDRDLGWMLHDIDFEHGNQPRFFRAEMKNGVIDVPPF
                     YAEEVKS"
     gene            complement(4540..6291)
                     /locus_tag="PXD66_001440"
                     /pseudo
     CDS             complement(4540..6291)
                     /locus_tag="PXD66_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250669.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
     gene            complement(6281..6742)
                     /locus_tag="PXD66_001441"
     CDS             complement(6281..6742)
                     /locus_tag="PXD66_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319734.1"
                     /translation="MFKPHGSLDWFMAGDVPMTSCIGKVEEPLIITPGVGKYKKGYGQ
                     PFDAHREKANEAIDSASSILCIGYGFNDDHLQTHLTGKIKSGVKTLLLTRGLSENARN
                     VVKEAPNCKALIFDGDKAGTRVVSKQEETFIPNVHWWDVEFFVKEILENGK"
     gene            complement(6901..8037)
                     /locus_tag="PXD66_001442"
     CDS             complement(6901..8037)
                     /locus_tag="PXD66_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL6319735.1"
                     /translation="MLLRKAFKFEIRPNGEQVRKMKQFCGCSRFVFNKALAWQNEQYE
                     QDNHHKFSYTKLANLLPQWKKELVWLKDCHSQVLQQSLKDLESAFKNFFQKRADLPKF
                     KKKGVKESFRFPQGCKIEQKNDRLYLPKIGWVRYRNSREIVGEVKNVTVSMKCGRFFV
                     SIQTEFKQEIPTHKGGEIGIDMGIARFATLSNGEFFEPLNAFKTHKGKLAKLQKQLKN
                     KVKFSKNWQKLKDKIGKLHHKIANCRKDFLHKISTQISKNHAMIYVEDLQVANMSKSA
                     KGTAEVHGKNVKQKSGLNRVILDQSWFEFRRQLDYKTQWLGGFLLAVPPQNTSRTCPC
                     CNYTAKENRQTQENFECVECGYTENADVVGAMNVLTRGQAIVQA"
     gene            8084..8500
                     /gene="tnpA"
                     /locus_tag="PXD66_001443"
     CDS             8084..8500
                     /gene="tnpA"
                     /locus_tag="PXD66_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005720092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDL6319736.1"
                     /translation="MKKETEIRRGRHVVFNLHVHLVFVTKYRREVFTQAILNDLHSIF
                     ATICADFEATLVEFDGEDDHVHLLVEYPPKVAISHLVNSLKGVSSRMIRKKNYPSIRK
                     KLWGNQLWSPSYFAGSCGGAPISIIRQYIEQQQTPD"
BASE COUNT         2337 a         1929 c         1629 g         2665 t
ORIGIN      
        1 ggagttttct ccaatcagga gcaattaaat tagcattact aatttgagca atccctccta
       61 atacaaatac aggaggaacc ccaatgtggg aagaaatggt tctattaaaa ttactacctt
      121 ttttatcatt aggcgtatgt tttaacttcc caatatttcc accactctca tcaagaaata
      181 ttttgtagtt atgctgtgct atatttaatc cgtgaaatga tctttctaag tttgaagtta
      241 tcacaacaag tccctcccca aagtaggtaa ttataataaa gatccaaaca ctcccaccac
      301 ataccccttc gatctatccc tttccaacaa ctttggttgg cagaggtcga taagcgagca
      361 ggctttgcag cgtttgctgt attctggagg aggtgtggtt ccgctatcta agagcgaccg
      421 cacttcggca atggtggcga gggttttggt gcgtagttcg gtggaaaaaa tgatcggcaa
      481 acggtggcga gtttgcatat accacaacgc cccttcgtta atcgtctgct ctgtcatttc
      541 ttccaaacat aaggcttggg cacagagttg gatttcatca atcggatcaa gtttgggctt
      601 gccacgtttg tattccactg gttttagctc gcctgttttt aggtctcgct caaccaaatc
      661 caaaatgccg cttatgccta atttttcggc tgcaacgtga accgttcgct caaagcgaat
      721 ccctttgcga acttctggct caccactatc tacccgctcg tgcaacgctt gaccttgtgc
      781 ggtgagatag ttttctgccc acacctgttc gttgtggatc agcgcacatt gacgtgggca
      841 gaaggcatag tgttgcaaag cggaaagcga aattgaccgc ttgtcatcgc caaattttgg
      901 ctgaacaagc ggtaaaattt ccgcagaatt ttgcaaatcg cccattacag taattcttca
      961 acggttacac ccgttaagcc ttcggtttgt acgctgatac agtaatcgtc atagtctttc
     1021 gctggcgtgt ctttttcacc gttgattcgc tcaactttta ccgagtcaaa cagtttgtgt
     1081 gcaggttggt tgcctaattc gctgtcgtgt ttaaatacga tcagtttacg agctgccatt
     1141 tcgcctcgtg cggcagaacg gtcgtgttca aacataagtt gcaaggcttg ccaaagtttt
     1201 tgtacatctt catcagaaaa gcccgttttt tcagcgagtt tagctgaaat aaagccgtga
     1261 acacgataaa gtgcgtaagg cacgatatat ttacgcccca tagtccgttc tttttcaaga
     1321 tctttttcgt tggtcactgc cattcgtgtg atcgatactt ctagcggaat aatcggatcg
     1381 atagattgag cgaacgccaa ttgcactggg cctcgcacct gtccgctgtt tacttcggta
     1441 gtcattaccg ccccaaaggt gcgaatatcg aaaaagtttt tgcacatcca agcggtaatt
     1501 tctcgggctt tagcttcctc ttttggtagt tttttcgctt ccgattcaat ccctaatgct
     1561 tcataagcac gtttgttttg taaattcaga acactttttt ctttaacata aatttcgtaa
     1621 cccgcctcgt tttcatagct catttcaata aaattgcgga ttttgcgttt taggcaaaca
     1681 tcggtgacta aacctttact ggtttcagga tcaaggcgtg gcatatttcc tgcatctgga
     1741 tcaccatttg ggttaccgtt ggttacatca aagaaaaaaa caaattcata gcggttttga
     1801 atagacatag tgtttcctta gttttcaata gactcagtgg tttctttttt agcgaaatag
     1861 ctttgttttt ggtggtaata accaatcgca aaacgccctt gttcatcgat acttaaatgg
     1921 cgagggaaat tttccggcaa gctggcaata atctcaccta aatccttatc cagattaacc
     1981 gccattccgc ccttatcttt tctcaagcga gataaatggt gttttgcacc gcttaataag
     2041 cgagggaata ccgaaaacgg cacggcagaa gccgaaccat aatagcggtc ggcaatgccg
     2101 gcatttaatt cgcctaatgc ttggctttgg attctttcta atactgcaaa taacctgccg
     2161 agcaagtaag cttggttatc gctttcttta tttaagctca taggaactcc ttctttaata
     2221 aaaccttttc taaattttcg ttgtaagact gctttcatca acgctactcg caaaccgtta
     2281 atatcgccgt ctgctcggat tcgagtcact aactgtgaaa gtaaactcat cggatataaa
     2341 tgaccgctaa taactgcgcg agccatttca cccgccaaga ctggagaaat attctcactt
     2401 ttacctaatg aagcggtttg taataatagt cgccaaatgg aaggtggtgt tttccatggg
     2461 caaggctcaa gagcgagatc ttgccaatgc tcgctgaggt tttttgccag ttgcccaaag
     2521 gtggtatcca gccaaaaacg taccgaaatg cgagctgcat ttggagcaag ccccaacaca
     2581 taaaaacggg tgttttcaga taattctggg gaaatttctg caagcggtcg tcctttgctg
     2641 attttttgca attcttcaaa gattttttgg ttttcttgct catcatctgg aggcgtcatt
     2701 aaagcagcaa aaaagccttc agcagcttca gcttgggtgg tattgtctgc ttctgcccaa
     2761 aagacagtgc tggcatcacc aatagttaaa cgatgtttct cggtgcgtaa caaataattt
     2821 aaagcagtag tgtaggcaaa ggcggcttgt tcagaaacag gggcattact accttgttcc
     2881 ttgccaaatg aagtgaagga ttctttattg aaagagatga ttgaaccacc ggaactttgc
     2941 ccaccaaata cccctttaat aggaggatga gttcgttgaa ttggcgataa tttgcctgaa
     3001 attaagcata aacctttttc agcattatca ctttgattta tcaacaaact tgtccaaagg
     3061 gtttgtgcag cttctcgctg atgaatataa ccgcttgtgc cgactaattt aaacacgaca
     3121 ttcgcatcaa gaatttctct tgggcagatt gattcggcaa aatgtgtggg ttgccattgt
     3181 tgcaggaaat ttcttatcgc cattaaacct tcatcattgg aatctgcaag caaatccaaa
     3241 tggtactgtt taaatgcctc aaaggttttc tcggcgaaat tgatgggttg atctttggcg
     3301 gtggctttat caggattact ttcaatgcct agcacataag cggttttatc ccacagaaaa
     3361 ttgggcttaa tgccagacgt gcgtttttca gggcgaggta cgctcatcaa tttcggtttt
     3421 ggtttcttat cttctgttaa atttgagatc acattgacca aatttccctt tgaatctaat
     3481 tctagtaccc aaccgatttt ttcttcactg aaaccataag aaggcacttt agcttcgcct
     3541 gttaagcgat cttcttgtgc tgcaaggcgg taataatagc gagttaaggc ggtaataata
     3601 gcgagttaag gcggagagaa tcatgatttg acctcctctg cataaaatgg tggcacatca
     3661 atcacgccat ttttcatttc cgcacggaag aaacgaggtt gattaccgtg ctcaaaatca
     3721 atgtcgtgca acatccagcc taaatcccga tctcgctcat tttctgccaa aaaacactca
     3781 ggtaacggtt catcgtcatc aatcaaggca aaatttgcgg caaattcacg cacgcccata
     3841 caaggctgct ggaaacattg cccctttttc gcacggcgtt taaacatttc aatatgcttg
     3901 gtgacggttt ccgcctcgcc tgccattttg gtcagcactg cgtgagcttc gatcacataa
     3961 ccgacatttt tcagcacagt agcagcacgt tgttggcggt cgtcttcaat cacagtatat
     4021 aaatccgcca ctgttttacg cttcattgca ccactgattt tactggcaga aattttactg
     4081 cctaactcat tacggcgaac agactcaaat tgaatcggtt gtaatacata aattttatca
     4141 atcacccagc gaattgccgg cttccaatgc accgcagcca aaatcccccg tgccgcagac
     4201 ggcgtaatca catcataaga cacccgttct accttcattt caggacgggt aaaacaagca
     4261 taatcacccc aaatatggag cctgattttg tttggcataa tagaatgctc cttttaatta
     4321 tgtaacacac acctccccga agggaggtga aatttcttta gcttatatta taactgcttt
     4381 ggtttcaacc cacgcactca gtaaaatgcg aaaaatttta tggcttatta tattaagcgg
     4441 tcggatttac ttagaaagtt gcaaagttat ttgagtattt aaccgcttgt atttcaaaaa
     4501 tagtcagaaa aataacagca ttctaggtac aatatttttc taagaacgaa cttgtctccg
     4561 caatgcctct actgcgtttg acacagcatt tggtttgggc tcctggtttg cccaatcaga
     4621 ccagaattgg cgtgtagcac tttctggggc tatatgcggt ttagcaagtt tgattcgtgt
     4681 aggtaataga atcgaatcgc ctagcaataa cgcttcgcct aaatcaagta acggcaatat
     4741 atcagtaatt ccaaccatag attccggcaa taaacgcttt acaacacttt gatcttgacc
     4801 atttgttaat cgtaaaacca agaaattatt acattgactc aaaattgttc ggcttacatc
     4861 tgaaggacgt tggctaacga ccaacaaaga cacgccatat tttctacctt ctttcgcaat
     4921 ccgctcaaag gcttccaatg cgcgtttttc cgctgcttcc gctgaatctc gaacagggag
     4981 atataaatgt gcttcatcgc aaataatcgt tagtggatgg cgggaatctt tatccatcca
     5041 aaattgaatg ttgtataaaa ggcgggcaag aactcccgta acaatcggta aaacatccga
     5101 cgggacttcc gaaaaatcaa taatttttat ccctttttcg ctctcagaag cagaaaacag
     5161 ttttgacaac atattttcca gccacccata gctgttgcac tctactggcg gttggaacat
     5221 aaaaccgcaa cgtctgtcat ctattttggc tttcatcctt cccataaacc tagacaactt
     5281 actataccaa ggaccttgct ttgttcctga acctccctct ttaggtacca tctcagaatt
     5341 atctttatct aaacgagcta acaataattc aatagaatac ggaattggcg aatcaacagt
     5401 aaacgtttcc aaaacatcat ttctttcttc cttattcaat gtttgctctt ttaatgcgcg
     5461 aacatgctca gtaaaacgcg ccgcctgatt cggagcatta tcatcactgc gatctaaaat
     5521 taaagccagc atttattccc gattgagcag ccaataggga ataaataaac tgcgttcatc
     5581 tgaaccatcc aaatctcccg gtccagcaat acggtattgc tgagcgatag cgtttttacc
     5641 ttcgcataga gtgctatatt caccgtgcat atcgaataca ataagattag cagcttttaa
     5701 agctgctgca cgttccaata ttaatgcaac tgaccagctt ttaccggaac cagtactacc
     5761 caaaatagca gcgtgccgtt gaaagaattt atctccgttt gctatagcta cagccgtttc
     5821 atcagccata aaatgaccta aagtcagccg ttcattttct tttacgttag cggacaaaag
     5881 attcataaat aattgcagat tgctgccact aatatgaaag caatctctat caatttgcgg
     5941 aaaacaatcc gctccccgct taaaagtgtt gctcaaacta ccatcaacag ctttataagt
     6001 accgattaat actgccttga taaagtcatc ctgactttca cccagaggaa tttcaccctc
     6061 attagatatt tcttctagca gcaatttttc actgatagaa cgcgttaccc gttcgataat
     6121 gccgataagg tactcactag ccgttctgcc ttgaatcgca atcagattac ccacagcaat
     6181 actagtcatt tgttgatgct cttcaacaga aatcaataca cggctggtat ctactgttac
     6241 aaccctgccc aaacgggcat tgtctttaaa attcaatgtt tcatttgcca ttctctaaaa
     6301 tctcttttac aaaaaactca acgtcccacc aatgaacatt aggaataaaa gtttcttctt
     6361 gcttgctaac cacccttgtt ccagccttat ctccatcaaa aatcagagcc ttacaattcg
     6421 gagcttcctt caccacgttt ctggcatttt ctgaaagtcc tcttgtcaga agtagtgttt
     6481 tcacaccact tttaattttg cctgtcaaat gtgtttgaag gtggtcatca ttaaaaccat
     6541 agccgataca taaaattgaa gaagcagaat caatagcttc atttgctttt tctcgatgag
     6601 catcaaatgg ttgtccatag ccttttttat acttcccaac accaggcgta ataattaaag
     6661 gctcttccac tttacctata caacttgtca ttggaacgtc tccagccata aaccaatcta
     6721 aactaccatg cggtttaaag attttgatat gatctcgttg acatcctccc ctgcctaaag
     6781 gcaggggatt cctactagct cccacagcaa gctgtgggct actctcggtg ggtttctact
     6841 gctgatctct attgagattc acttcataga cgcttcgggt atgtcctacc ctgatattta
     6901 ttatgcttgg actattgctt gcccacgtgt aagaacattc attgctccca ctacatcggc
     6961 attttccgta tagccacatt ctacgcattc aaaattttcc tgtgtttggc gattttcttt
     7021 tgctgtatag ttgcaacaag ggcaagtacg actggtattt tgtggaggaa ctgctagcaa
     7081 aaatccgcct aaccattgcg ttttgtagtc cagttgtcgt ctaaattcaa accaagactg
     7141 gtctaatatc actcggttta agcctgattt ctgtttcaca tttttgccgt gtacttccgc
     7201 tgtgcctttg gctgatttgg acatattcgc cacttgcaaa tcttcaacat agatcatcgc
     7261 gtggtttttg ctgatttgcg ttgagatttt atgcaggaaa tcttttcggc agttggcgat
     7321 tttgtgatgt aacttgccga ttttatcttt cagtttttgc cagtttttac tgaacttcac
     7381 tttatttttt agctgttttt gcagttttgc caattttcct ttgtgggttt taaacgcatt
     7441 taacggctca aaaaattctc cattggaaag ggttgcaaaa cgagcaatgc ccatatcaat
     7501 gccgatttct ccgcctttat gcgtgggaat ttcctgttta aattccgttt gaatgctcac
     7561 aaaaaagcgt ccgcatttca ttgaaacggt gacatttttt acttccccaa caatttcacg
     7621 actgttgcga taacgcaccc aaccgatttt cggcagatac aagcggtcgt ttttctgctc
     7681 gattttgcaa ccttgcggaa aacggaaact ctcttttacg ccttttttct tgaatttggg
     7741 caaatccgca cgcttttgaa agaagttttt aaacgcactt tcaaggtctt ttaacgactg
     7801 ttgaagcact tgtgaatggc aatctttaag ccataccaat tcttttttcc attgcggaag
     7861 caagttggcg agcttggtat aactgaattt gtgatggtta tcttgttcgt actgctcatt
     7921 ttgccacgcc aatgccttgt taaacacaaa gcgagaacag ccgcaaaact gcttcatttt
     7981 gcgaacttgt tccccatttg gtctgatttc aaatttaaag gctttacgta gtaacattgc
     8041 acgaattaga atacaaaaat tgtgcttatt ctacacttgg cctatgaaaa aagaaacaga
     8101 gattagacgt ggtcgccacg ttgtttttaa cttacacgtt catttagttt tcgtgactaa
     8161 atatcgcagg gaagttttta ctcaagcaat tttgaacgac ttacactcaa tttttgcaac
     8221 gatttgtgct gattttgaag ccacattagt ggaatttgat ggtgaagatg atcacgttca
     8281 tctacttgtg gaatatccgc ctaaagtcgc tatttctcat cttgtaaaca gtttaaaagg
     8341 cgtttcgagc cgaatgatta gaaagaaaaa ctatccgagc attcgcaaaa aattatgggg
     8401 aaatcaactt tggtcaccgt cttactttgc tggtagttgt ggtggtgcac caatatcgat
     8461 tattcgccaa tatatcgagc aacaacaaac acctgattag ttagaaattt gaaacggctt
     8521 tgccgtttat accttatatc cacgccctaa aggacgtggt
//
LOCUS       DANLXF010000097         8264 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.097, whole genome shotgun sequence.
ACCESSION   DANLXF010000097 DANLXF010000000
VERSION     DANLXF010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8264
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.097"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            3..719
                     /gene="arcA"
                     /locus_tag="PXD66_001444"
     CDS             3..719
                     /gene="arcA"
                     /locus_tag="PXD66_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HDL6319737.1"
                     /translation="MPQILIVEDELVTRNMLKSIFEGEGYQVFQASNGEEMHSVLSTN
                     EINLVLMDINLPGKNGLILAREIRENMKLPLMFLTGRDNEIDKILGLEIGADDYITKP
                     FNPRELTIRARNLLQRTMIDKENDVVKEQSEIESYRFNGWILDINSHSLISPEGEVNK
                     LPRSEFRALLHFCQHPGKIQTREDLLKKMTGRELKPHDRTVDVTIRRIRKHFEDHPNT
                     PEIIVTIHGEGYRFCGNIEP"
     gene            739..1257
                     /locus_tag="PXD66_001445"
     CDS             739..1257
                     /locus_tag="PXD66_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HDL6319738.1"
                     /translation="MKKLTIVALTCLFSTHLLAAPVGNTFTGIGVGVDVTTTKYKVEG
                     VKGKDSTGPALVVDYGIEQGNNFVGVVQGKAKVGSTKVFSDVKQKNKYTIAYQQGYRV
                     GSDLLPYVKVDASSSKIGNETYRGFGFGAGAKYAVSSDVELGAEYTRDNLKRSGTKLK
                     GNAFTANATYRF"
     gene            complement(1335..2564)
                     /gene="serA"
                     /locus_tag="PXD66_001446"
     CDS             complement(1335..2564)
                     /gene="serA"
                     /locus_tag="PXD66_001446"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDL6319739.1"
                     /translation="MAKISLDKSKIKFLLLEGVHQNAIDVLLAAGYTNIEVHKKALDG
                     QELIDAIKDAHFVGIRSRTFLTEEVLAQAPKLIAIGCFCIGTNQVDLNAAKRRGIPVF
                     NAPFSNTRSVAELVLAEMILLMRKVPTANAEVHRGVWNKSAAGANEVRGKKLGIVGYG
                     HIGSQLSILAEAIGMQVYFYDVENKLPLGNAQQVPTLEHLLSTCDMISLHVPEIDSTK
                     NLMSAERIAQLKEGSVLINAARGTVVDLDALAARIEDGSLRGAAIDVFPEEPASINDP
                     FVSPLCKFDNVILTPHIGGSTSEAQANIGTEVANKFVKYSDNGSTVTAVNFPEVSLPL
                     HTNTKRLLHIHENRPGMLNQINQIFVESNVNIAAQYLQTDAAIGYVVIDVESDNVDEA
                     LQRLKAIEGTIRARVLY"
     gene            complement(2601..3260)
                     /gene="rpiA"
                     /locus_tag="PXD66_001447"
     CDS             complement(2601..3260)
                     /gene="rpiA"
                     /locus_tag="PXD66_001447"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDL6319740.1"
                     /translation="MDQQEMKKIAAQAALKFVKPDTIVGVGSGSTVNCFIDALASMKD
                     EIKGAVAASKASEQRLRDIGIEVFSANEVTELDVYIDGADEITPQGAMIKGGGAALTR
                     EKIVSSLAKKFICIVDKSKQVDVLGSTFPLPVEVIPMARSYVARQLVALGGSPEYREG
                     VVTDNGNVILDVHNFKIMEPLKMEHTINNIAGVVTNGIFAQRFANVTIVGTPEGAVIL
                     E"
     gene            complement(3405..4739)
                     /gene="glmM"
                     /locus_tag="PXD66_001448"
     CDS             complement(3405..4739)
                     /gene="glmM"
                     /locus_tag="PXD66_001448"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015939592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDL6319741.1"
                     /translation="MARKYFGTDGVRGEVGKFPITPEFALKLGWAAGKVLATQGTKKV
                     LIGKDTRISGYMLESALEAGLTAAGLSAVFVGPMPTPAIAYLTRTFRAEAGIVISASH
                     NPYYDNGIKFFSSVGEKLPDEIEEAIEAMLDKPMDCVDSANLGRASRINDAAGRYIEF
                     CKGTFPSELSLEGYKIVVDCANGATYHIAPNVMRELGAEVLEIGTKPDGLNINEKCGA
                     TDIKALQKVVVEAGADVGLAYDGDGDRLIMVDHLGNKVDGDQILFILAREALRSGKLQ
                     GGVVGTLMSNMSLELALKELAIPFSRANVGDRYVLEQLKEKGWKLGGENSGHIIVLDK
                     NTTGDGIIASLQVLAAMASHKLSLNDLAKAVPLFPQVLLNVRFEKGAANPLESNEVKA
                     VAADVEKRLAGKGRILLRKSGTEPLIRVMVECEDAVLAQSCAEEIVEAVKRN"
     gene            complement(4765..6297)
                     /gene="ilvA"
                     /locus_tag="PXD66_001449"
     CDS             complement(4765..6297)
                     /gene="ilvA"
                     /locus_tag="PXD66_001449"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HDL6319742.1"
                     /translation="MMNETQHSQSAIDYLRSILSSNIYDLAQVTPLQKMEKLSERLGN
                     SIFIKREDRQPVHSFKLRGAFAMISNLSKAQKEAGVIAASAGNHAQGVALSAKHLGLR
                     ALIVMPQNTPAIKVDAVRGYGGEVLLYGANFDEAKAKAIELSTELGMTFIHPFDSPAV
                     IAGQGTIGLELVQQKPDLDYVFIPVGGGGLIAGIAVLIKQVMPNIKVIGVESKDSACL
                     YHALKQGQPTDLERVGLFADGIAVKRIGDETFRLCQQYVDDVVLVDNDEICASMKLIF
                     ENVRAISEPSGATSLAGLKKYVKEHQLQDKNLACILSGANVNFHTLRFVSERCEIGEK
                     REALLAVTIAEQKGSFLKFCEILGNRAVTEFNYRHSDDKQACIFVGVRISGSAEKVEI
                     IKDLQQNGYDVADLSDDDIAKTHIRYMVGGRASSIQYEQLYSFEFPEQKGALLKFLQM
                     LTNWDISLFHYRAHGADYGDILAAFALNRGDEVELNRHLEQLGYRFQNVSESPAYQYF
                     LK"
     gene            complement(6558..7151)
                     /gene="slyD"
                     /locus_tag="PXD66_001450"
     CDS             complement(6558..7151)
                     /gene="slyD"
                     /locus_tag="PXD66_001450"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL6319743.1"
                     /translation="MNITKDLVPSIAYQVRTQEGVLVDEAPVNQPLEYLHGHNNLVEG
                     LEKALEGKAVGDVFEVRVKPEEGYGEYNDNMVQRVPKDVFMGVDELEVGMRFIADTDL
                     GPLPVVITEVDGDEVVVDGNHMLAGQELLFSVEVVGIREATAEEIAHGHVHGGHEHGC
                     GCGGHDHHHDHDGCCGGHDHDHHHHHGHGGCGCGGHH"
     gene            complement(7254..7394)
                     /locus_tag="PXD66_001451"
     CDS             complement(7254..7394)
                     /locus_tag="PXD66_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319744.1"
                     /translation="MKKLIKVCGLISATIFLSGCLVTSVVGGVVGTAVDVVDTVTPDI
                     ID"
     gene            complement(7466..8152)
                     /gene="aqpZ"
                     /locus_tag="PXD66_001452"
     CDS             complement(7466..8152)
                     /gene="aqpZ"
                     /locus_tag="PXD66_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HDL6319745.1"
                     /translation="MKKYFAEFFGTFWLVFGGCGSAVLAAGIPELGIGYAGVSLAFGL
                     TVLTMAYAVGHISGGHFNPAVSIGLLVGGRFNAKDLVPYIIAQVIGAIAAGAVLYTIA
                     SGVPTFDATAGFAGNGYGEHSPHGYSLTAALLIEVVLTAFFLIIIMGATDKRAPAGFA
                     PIAIGLALTLIHLISIPVTNTSVNPARSTGVALFQGSWAIEQLWLFWVAPIVGAIIGA
                     VVYRFIAEEK"
BASE COUNT         2427 a         1924 c         1559 g         2354 t
ORIGIN      
        1 agatcccaca aatcttaatt gttgaggatg agttagtaac aaggaatatg ctgaaaagca
       61 tttttgaagg agaaggatat caggtatttc aagcatctaa cggcgaggaa atgcactctg
      121 ttctgagtac aaatgaaatc aatcttgtgc taatggacat taacttaccg ggaaaaaatg
      181 gattaattct cgcccgagaa attcgagaga atatgaagct accattaatg tttttaaccg
      241 gtcgcgacaa tgaaattgat aagatcctcg gtttagaaat aggtgccgat gactatatca
      301 ctaaaccgtt taatcctcga gaattgacta tccgagcgag aaatttattg caacgaacga
      361 tgatagataa agaaaacgac gttgtaaaag agcaatctga aattgaaagc tatcgcttta
      421 acggttggat cttagacatt aacagtcata gtttaatctc accagaaggt gaggtaaata
      481 aattaccacg tagtgaattt agggctttat tacatttttg ccaacatcct ggaaaaattc
      541 aaacacgtga agatttattg aaaaaaatga ccgggcggga actaaaacct catgatagaa
      601 ccgttgatgt tactattcgc cgtattcgta aacattttga agaccacccc aataccccag
      661 aaattattgt caccattcac ggtgaaggct accgtttctg tggtaacatc gaaccttaat
      721 caagatagga gtaattttat gaaaaaatta actattgtcg cattaacttg cttattttct
      781 acccatctat tagccgctcc tgtcggaaat acatttactg gcatcggtgt cggtgtagat
      841 gtaactacaa caaaatacaa agttgaagga gtaaaaggca aggattccac aggtccggca
      901 ttagttgtcg attatggtat tgagcaaggc aataactttg taggcgttgt acaaggaaaa
      961 gcaaaagtag gtagtacaaa agtatttagc gatgtaaaac aaaaaaataa atatactata
     1021 gcttatcaac aaggttatcg tgttggatcc gatttattac cttatgtaaa agtagatgcc
     1081 agctcaagca aaatcggtaa tgaaacctat cgtggctttg gtttcggtgc tggtgcaaaa
     1141 tatgcagttt caagcgatgt tgaattaggt gcagaataca ctcgcgataa tttaaaacgt
     1201 agcggcacaa aattaaaagg taacgctttc accgcgaatg caacttatcg tttctaatct
     1261 tagcggtaaa gttttctgaa aaaataaccg cttgtaacac aaaagagcct gaagtgattc
     1321 aggctctttg ccttttaata taatactcgg gctcgaatcg tgccttcgat cgcttttaaa
     1381 cgttgtaatg cttcatcaac attgtcgctc tctacatcaa ttacaacata accgatagct
     1441 gcatcggttt gtaaatattg agcggcaata ttcacatttg attccacaaa aatctggtta
     1501 atttgattta acatacctgg gcgattctcg tgaatatgta ataaacgttt ggtattagta
     1561 tgaagcggta aggatacttc agggaaattc actgccgtca cggttgaacc gttatctgaa
     1621 tatttcacaa atttattggc aacttcggtg ccgatatttg cctgagcttc agaggttgaa
     1681 ccaccaatat gtggcgttaa aatcacgtta tcaaacttgc acaatggtga cacgaacggg
     1741 tcattaattg atgccggttc ttccggaaat acgtcaatcg ccgcaccacg caatgagccg
     1801 tcttcaatcc gagcggctaa tgcgtctaaa tcgaccactg taccacgagc ggcattaatt
     1861 aacaccgagc cttcttttaa ttgagcaatg cgttctgcac tcattaggtt tttagtcgag
     1921 tcgatttccg gaacgtgtag tgaaatcata tcgcaggttg aaagcaaatg ctctaatgtc
     1981 ggcacttgct gtgcattacc gagtggtagt ttgttttcaa catcatagaa ataaacctgc
     2041 ataccaatgg cttcggcaag aatactaagc tgtgaaccga tatgaccata tcccacaata
     2101 cctaatttct tgccacgtac ttcgtttgca cctgctgccg atttattcca caccccacga
     2161 tgcacttccg catttgcagt cgggacttta cgcattaata aaatcatctc tgccagcact
     2221 aattctgcca ccgagcgggt atttgagaac ggtgcattga ataccggaat acctcgtctt
     2281 ttcgccgcat tcaaatcaac ttggtttgta ccgatacaga aacaaccaat cgcaattaat
     2341 ttcggagctt gagccagtac ttcttcggtt aaaaaagtac gcgagcgaat accaacaaag
     2401 tgtgcatctt taattgcatc aattaattct tgaccgtcta atgctttttt atgcacttca
     2461 atattggtat aaccagccgc taagagtaca tcaattgcgt tttgatgtac gccctcaagc
     2521 agaaggaatt tgatcttgga tttatccaaa gaaattttag ccattttttg ctcctaagta
     2581 tttgtatttg caataataaa ttattctaaa ataactgcac cttcaggtgt gccgacaatg
     2641 gttacattgg caaaacgttg tgcaaaaata ccgttagtca ccacgcctgc aatattatta
     2701 atggtatgtt ccattttcaa cggctccata attttaaagt tatgtacatc taaaatcaca
     2761 ttgccgttat cagtgaccac gccctcacgg tattccggcg aaccacctaa ggctacgagc
     2821 tgacgagcca cataagaacg agccattggg atcacctcca ccggcagagg gaaagttgaa
     2881 cccagcacat caacttgttt ggatttatcc acaatgcaga taaatttttt cgctaatgaa
     2941 ctcacgattt tctcacgggt aagtgctgcc ccgccacctt taatcatcgc cccttgtggc
     3001 gtgatttcat cagctccgtc aatatacaca tctaattccg tcacttcatt ggcactgaat
     3061 acttcaatgc cgatatcacg caaacgctgt tccgaggctt tagaggcagc aaccgctcct
     3121 ttaatctcat ctttcattga agcaagggca tcaataaaac agtttacggt tgaaccactt
     3181 cccaccccaa caatagtgtc cggttttaca aattttaatg cagcttgtgc cgcaattttt
     3241 ttcatttctt gttgatccat ttttacctcc acttgtaggg caaatcatca tttgcccact
     3301 tcggcaaggc gaaaaaaatt ttcgccctac tacatctatt ctacacatat atgtaacaag
     3361 cggtcatatt tggcaaattt tttaccaaaa acgaccgctt gtcattagtt acgttttact
     3421 gcttcaacaa tctcttccgc acagctttga gcaagtacag catcttcgca ctccaccatc
     3481 actcgaatta atggctcagt gcctgattta cgcaataaaa tacgcccttt gcctgccaag
     3541 cgtttttcca catctgccgc taccgctttc acttcgttac tttcaagcgg atttgccgcc
     3601 cctttctcaa agcgaacatt tagcagaact tgcgggaaca gaggcactgc ttttgccaaa
     3661 tcattcaggc ttaatttgtg gcttgccatt gctgctaata cttgcagtga ggcaataata
     3721 ccatcaccgg tggtgttttt atctaacacg ataatatgcc cggagttttc gccacccagt
     3781 ttccagcctt tttctttaag ctgctctagt acatagcggt cacccacgtt agcacgagag
     3841 aatggaatgg ctagctcttt taaagctaat tccaagctca tattactcat taatgtaccg
     3901 actacaccgc cttgtaattt gcctgaacgt agagcttctc gggctaaaat aaatagaatt
     3961 tggtcgccat caactttatt gcctaagtga tcgaccataa tcaaacggtc gccatcacca
     4021 tcgtaagcta gaccaacatc cgcacctgct tctaccacca ctttttgaag tgctttaata
     4081 tccgttgcac cacatttttc gttgatgttt aaaccgtccg gcttagtacc gatttcaagc
     4141 acttctgccc ctagctcacg cattacattc ggcgcgatat ggtaggttgc accgttggca
     4201 caatccacca caattttata gccttcaagg cttagttcag acgggaaagt gcctttacaa
     4261 aactcgatat aacgtcctgc ggcatcatta atacgactgg cacgacctaa atttgctgaa
     4321 tctacgcaat ccataggctt atcaagcatt gcttcaatcg cttcttcaat ttcatcaggt
     4381 agtttttcac caaccgacga gaagaattta atgccgttat cgtaataagg attgtgagag
     4441 gcagaaatca caatgccggc ttccgcacgg aaagtacggg ttaaataagc aattgccgga
     4501 gtcggcatcg gaccaacgaa tactgctgat aaaccggctg cggttaaacc ggcttccaga
     4561 gccgattcca acatataacc ggaaatacgg gtatctttac cgattaatac ttttttcgtg
     4621 ccttgggttg ccaacacttt acctgctgcc caaccgagtt ttaatgcaaa ttcaggtgta
     4681 attggaaatt tacctacttc accacgcaca ccgtctgtgc cgaaatattt acgagccatt
     4741 ctaattccta agattgataa aaatttactt taaaaaatat tgatatgccg gactttcgct
     4801 cacattttgg aagcggtaac ctagctgttc taaatgtcgg tttaattcta cttcatcgcc
     4861 tctgtttaat gcaaatgcgg caagaatatc gccataatcg gctccgtgag ctcggtaatg
     4921 gaataacgag atatcccagt tagtcagcat ttgtaagaat tttagcaatg cccctttctg
     4981 ctctggaaat tcaaagctat agagttgctc atattgaatg gagcttgctc taccgcctac
     5041 catataacga atatgggttt tggcaatatc atcgtccgat aaatctgcca catcatagcc
     5101 gttttgttgc agatctttaa taatctctac tttttctgcc gagcctgaaa ttcgcacccc
     5161 cacaaaaata caggcttgtt tatcatcgga atggcgatag ttaaattccg ttaccgcacg
     5221 gttgcctaaa atctcgcaga attttaagaa actacctttc tgttcagcaa tggtgactgc
     5281 caataaagct tcacgtttct ccccgatttc acaacgctct gacacaaaac gtaaggtgtg
     5341 gaaattcaca ttcgccccgg aaagaatgca agctaagttt ttatcttgta attgatgctc
     5401 tttcacatat tttttcaaac cggcaaggga agttgccccg gacggctcgg aaatcgcacg
     5461 cacattttca aaaatcaact tcattgaggc acagatttca tcattatcca ccaacaccac
     5521 atcatcaaca tattgctgac acaggcggaa agtttcatcg ccaatgcgtt ttaccgcaat
     5581 accgtcagca aaaagcccaa cccgttctaa atcggtcggt tgcccttgct ttaaggcgtg
     5641 atataagcaa gcagaatctt tagattcgac cccaatcact ttaatattcg gcatcacctg
     5701 cttaattaaa accgcaatgc cggcaattaa gccgccaccg cccactggaa taaatacata
     5761 atctaaatcc ggcttttgtt gcaccagttc taagccgatt gtgccttgcc ctgcaatcac
     5821 tgccgggctg tcaaacgggt gaatgaaggt catacccaat tcggtggaaa gctcaatcgc
     5881 tttcgcctta gcttcatcaa aattcgcccc ataaagcaac acttcaccac catagccacg
     5941 taccgcatca accttaattg ccggtgtgtt ttgcggcatt acaatcaagg ctcttaaacc
     6001 taaatgtttg gcagaaagtg ccaccccttg tgcgtggttg cctgctgaag ccgcaatgac
     6061 tccagcctct ttttgtgctt tggataaatt tgagatcatc gcaaaagcac ctcgcaattt
     6121 aaagctgtga accggctgtc ggtcttcacg tttgataaaa atagagttgc ctaaccgctc
     6181 agacagtttt tccattttct gtaaaggggt cacttgcgct aagtcataaa tatttgagct
     6241 taaaatcgag cgtaaatagt cgatagcgga ttgggaatgt tgtgtttcgt tcatcatttt
     6301 gaaaggttat ttattgttag attgagataa agacattggt aaatttccaa ggaaaattga
     6361 ccgcttgttt ggttatttag caatggcttt aacagggaat atttcctctc gcccgtttgc
     6421 gggagagaga cagcttaaaa tttcgttcag aaattttaag cagagagagg ggaaaccttg
     6481 gatatccccc tctccctccg caaatcttcg atttgctcca ccctctcccg caagcgggga
     6541 gaggggaaat cacaaagtta gtgatgacca ccgcaaccac agccaccgtg tccgtgatga
     6601 tggtgatgat cgtggtcgtg accgccgcag caaccatcgt gatcgtgatg atgatcgtgc
     6661 ccaccacaac cgcaaccgtg ttcgtgtccg ccgtgaacgt gtccgtgtgc gatttcttcc
     6721 gctgtcgctt cacgaatgcc aaccacttcc acagagaaaa gtaattcctg accggctaac
     6781 atatggttac cgtccaccac aacttcatcg ccatctactt cggtaattac caccggtagc
     6841 ggacctaaat ccgtgtccgc aataaaacgc atacccactt ctaattcatc gacgcccata
     6901 aatacatctt tcggtacacg ttgaaccata ttatcgttgt actcgccgta accttcttcc
     6961 ggtttcacac gcacttcaaa cacatcgcct acagctttgc cttcaagggc tttttctaag
     7021 ccttctacca agttgttgtg accgtgtaaa tattctaacg gttggtttac cggtgcttca
     7081 tctactaata cgccttcttg agtacgcact tgatatgcaa tgcttggcac taaatctttt
     7141 gtaatgttca tattgaatcc tttaattcat cggttttata aaatagcaag ccattgtaac
     7201 gctttatttt atatttgcaa tctatctgga aattttaacc gcttgtcagt aaattagtcg
     7261 ataatatcag gggttacggt gtctaccaca tcaactgcgg ttcccaccac tccaccaacc
     7321 accgaagtta ccaaacaacc acttaggaaa atagttgctg aaatcagccc acatactttg
     7381 attaattttt tcattttaaa atcctttcaa aaacaaaaaa cagccctaat caagactaga
     7441 gctgcatcat atttattcgc ttaaattatt tttcttcagc gataaagcga taaactaccg
     7501 caccgataat tgcaccgaca atcggagcaa cccagaacaa ccataattgc tcaatcgccc
     7561 agctgccttg gaataacgct acaccggttg aacgtgccgg gtttactgag gtattggtta
     7621 ccggaatgct gattaagtgg attaaggtta aagccaaacc aatagcaatc ggagcgaaac
     7681 ctgctggagc acgtttatcg gttgcaccca taatgatgat taagaagaat gccgttaata
     7741 ccacttcaat taataatgct gctgttaagc tatagccgtg tggcgaatgc tcaccataac
     7801 cgttgccggc aaagcctgct gtagcatcaa aagtcggaac acctgaagca atggtgtata
     7861 acactgcacc ggcagcaatc gcaccgatta cttgggcaat gatataaggc actaaatctt
     7921 tagcgttaaa acgaccgccg actaataaac cgattgaaac cgccgggttg aagtgaccac
     7981 ctgaaatatg accgactgca tacgccattg ttaatacggt taaaccgaag gctaacgaaa
     8041 cgcctgcata accgattcct aattcaggaa tgcctgctgc taaaactgca ctgccgcaac
     8101 caccaaacac taaccaaaat gtcccgaaaa actcagcaaa atattttttc attttttatc
     8161 cttaccctaa agggtgaatc acacataagt gtattaactt tccgaatgct ataaaacata
     8221 gccaataaaa tatgagtata ctaaaaaaag taaagacgca tatt
//
LOCUS       DANLXF010000098         8240 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.098, whole genome shotgun sequence.
ACCESSION   DANLXF010000098 DANLXF010000000
VERSION     DANLXF010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8240
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.098"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..445)
                     /locus_tag="PXD66_001453"
     CDS             complement(<1..445)
                     /locus_tag="PXD66_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDL6319746.1"
                     /translation="MPFYAGQVKCIFIDPPYNTKSAFTHYDDNLEHSIWLSMMYPRLE
                     ILRELLAEDGSIWITLDDNESHYLKIMLDEIFGRKNFISNLIWEKKRKPSFLGKVGKV
                     TDNLFCYAKNKNELNELNDLTYLETSGDETYPLYNAGNSREILVFP"
     gene            complement(835..3321)
                     /gene="torA"
                     /locus_tag="PXD66_001454"
     CDS             complement(835..3321)
                     /gene="torA"
                     /locus_tag="PXD66_001454"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HDL6319747.1"
                     /translation="MKKQDNIDVSRRGFLKNSSLSLAAGAGLASGTTGVVGALASTSA
                     KAAEMKTVVTAAHWGPLGVVVENGKVVKSDSVFEISVPNELQHVVADQVHGETRVKYP
                     MVRKGYLEGNKDTTLRGRDEWVRVSWDKAFELVANEMKRVREAHGANAIFGGSYGWYS
                     SGALHASRTLLQRYLNVTGGFVGVKGDYSTGAAQVIMPHVLGTIEVYEQQTSWEVILE
                     SSDIVVLWSANPLNTLRIAWTSTDQQGIEYFKKLKESGKRIICIDPVKSESCEFLGAE
                     WVPVNTGTDVALMLGMAHTLVAEGKHDQAFLKKYTTGFEKFEEYLLGKTDNQPKDAKW
                     ASQICGVPEETIKKLATDFQSKRTMLMAGWGIQRQRHGEQGHWMLVTLAAMLGQIGLP
                     GGGFGLSYHYSNGGVPSATGGILGSITANPAVEAGAKTWLDETSKSSFPVARISDALL
                     NPGKTIDYNGTQITYPDIKIVYWAGGNPFTHHQDTNTLVKAWQKPDTIIVNECHWTPT
                     ARMSDIVLPATTSYERNDLTMSGDYSMMNIFPMKQVVEPQFEAKNDYDIFAELAKRAG
                     KEAEFTEGKSEMDWLQSFYQTAFEAARKNRVIMPKFEKFWQENKPLSFKASDKAKKWV
                     RYEEFRNDPLLNPLGTPSGKIEIFSEVIAKMNYDDCKGHPTWMEHEEYSGKTTAEEPL
                     ALVTPHSKWRLHSQLAYSSLRKQYAVNDREPVLIHTEDAAARGITSGDIVRIFNKRGQ
                     VLAGAVVTDGIIKGTLALHEGAWYDPLDLGTSEQPLCKNGNPNVLTRDEGTSKLAQGN
                     SPNTATVQVEKFTGQAPEVTVFKEPKYTQG"
     regulatory      complement(3340..3536)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            complement(3540..4655)
                     /locus_tag="PXD66_001455"
     CDS             complement(3540..4655)
                     /locus_tag="PXD66_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HDL6319748.1"
                     /translation="MAKMKKIAVFVGIMALGAAVLGGTQYAMKATSSTEFCVSCHSMT
                     HPQAEWEGSVHFSNRKGIRAECSDCHLPQDGLHYVKAKIIALKDVWHTVVTNKLPDQE
                     AYEAHRLEMAQRVWAEMKENDSMTCKSCHSFDAMVLSEQTEAAQKMHKLAQETNQTCI
                     DCHKGIAHFMPDVPVDNAAAMGELAKHGAEFSTNDKTLYTLAMTPVQLAQGGEIRLLP
                     YAELHQWTAEGEQVKATVNGWQQEGAESVVYMDLGKRIMVALLPDDAQSKVNVTKTVF
                     DNVTNSNWKEINFEITAPKNAVTANITALNHFGDNLNQTHCSGCHAPIGAEHYTANQW
                     IGVVNSMKDRTSMSADDVRAVTIYLQRNAKDVAGSSH"
     gene            complement(4797..6548)
                     /locus_tag="PXD66_001456"
     CDS             complement(4797..6548)
                     /locus_tag="PXD66_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL6319749.1"
                     /translation="MDKRSRWSCLVNEQGEISEWEDKAGADYFAELPQICHYAQALLY
                     SDKNSLQFYYSSNHQWLKISLERHQSFVLTQAEHTDLPYGLSKREIELLTLISSGLSN
                     EDIANELCISKRTVDKHIENIFAKTHTKSRTLLAVFAITEHLICLPTPGKLTQSILAS
                     FNIEQLALQIIAKKQPLVTASTRHEPPIFTINHHHSRPIIIGVPYVEQGIGQIDTREL
                     LNGTQLAVETINQQGGIHGREVQVATAGFYVKDKASILQAYHHLFDQEVDAISTAYAC
                     YLPEVHELIATKGIPYLHIATHSGSNKLAQNLPNQRIENMFQVCASDVTYGLGISHFL
                     QYYRYHYAPWLQNKTVMVVTVSWQKVDIGIESLTLSLREQGWNVDYLELTQEQDNYAT
                     LMAQVHNLAPSIIVFASYFAEDIVQFYQTFIRNPTNAIIYSIYAPSAFLPHEKLCEGV
                     LWSTNSGLSENYLGRQFSQHYERFFGKSPTYSQASIAYDQANILANAWKQSVSPRHFK
                     AVSNAIRLQPHYGVNGTYYFNTDSQIGLTYAETHDLSISLPQLVYQIQQGQSRVIAPE
                     LFANAQFILPPWFSEKA"
     regulatory      6712..6868
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            6857..7882
                     /gene="moaA"
                     /locus_tag="PXD66_001457"
     CDS             6857..7882
                     /gene="moaA"
                     /locus_tag="PXD66_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDL6319750.1"
                     /translation="MQSIIPIKNVGDDVVSGLVDRFQRQYTYLRLSITDVCNFRCNYC
                     LPDGYKPPSHKQTFLSVDEIQRVAKAFANLGTEKIRITGGEPTLRKDFLEIAHTISQT
                     PGIRKVALTTNGYRLERDVELWQQAGITDINVSVDSLDPRQFQLITGENKLQSILKGI
                     DKAFEIGYPKIKVNAVLMKQYTAPELDKFLTWIKDKPIQMRFIELMETGEMDSFFQAQ
                     HLSGQSVMTRLLQEGWQLQPKAMSDGPAKVLSHPDFKGEIGLIMPYEKNFCASCNRLR
                     VSALGKLHLCLFGEEGIDIRDLLASDEQQLQLETRLKSSLQGKREHHYLHIGDSGVRN
                     NLASIGG"
BASE COUNT         2280 a         1754 c         1684 g         2522 t
ORIGIN      
        1 gagggaaaac taaaatttct cttgaatttc cagcattgta taatggatag gtttcatcac
       61 ctgaggtctc aaggtaagtt aagtcattta attcatttaa ttcatttttg ttttttgcat
      121 agcaaaataa attatctgta actttaccaa ctttgcctaa aaaagatggt ttccttttct
      181 tttcccaaat taagttagaa atgaaattct tccgcccaaa aatttcatca agcataattt
      241 tcaaatagtg actttcgtta tcatcaagag tgatccaaat tgaaccatct tcggcaagta
      301 actctcgcag aatttcaagg cgtggataca tcattgaaag ccaaatggaa tgctctaaat
      361 tatcatcgta gtgggtaaag gccgattttg tgttataggg aggatcaata aaaatacatt
      421 tgacttgccc cgcataaaag ggcaaaagag attttaaggc tgataaatta tcaccctgaa
      481 taattaaatt ttgcttgact ttagataagg tttcagcaga agctgagctg tcggctgtcg
      541 gctgtcggct gtcggctgtc ggctgtcggc tgtcggctgt cggctgtcgg gcaatattat
      601 gcgagaagtc cggcaactca cgcaataggc aatatggggt ttgttttgct tttttgacgg
      661 cttcttcttt gccgtaccaa gtaagggttg tcataaaatg cacctgaaaa gtaaaagtgg
      721 attattgtaa caagcggtca aaaaaataga aagttttaca aaaaattaag caaatttaac
      781 cgcttgcaac atagaaaagg cacagtttta tactgtgcct ttggttatat gaaattagcc
      841 ttgtgtgtat ttaggctctt taaatactgt tacttctggt gcttgtccgg tgaatttttc
      901 aacctgtacc gtagcggtat ttggtgagtt accctgtgct aattttgatg tgccttcatc
      961 acgggttagc acgttagggt tgccgttttt acatagcggt tgctcgctgg tgccgagatc
     1021 gagcggatcg taccaagctc cttcgtgcaa ggctaatgta cctttgataa tgccatctgt
     1081 caccaccgca ccggctaata cttgaccacg tttgttaaaa atacgtacaa tatcaccgct
     1141 tgtaatgcca cgagcggcgg catcttcagt atggatcaac accggctcac ggtcattaac
     1201 cgcatattgt ttgcgtaatg atgagtaagc taactggctg tgtaaacgcc atttagagtg
     1261 tggagtcacc aatgccaacg gttcttctgc ggtggtttta cccgagtatt cttcgtgttc
     1321 catccaagtt gggtgacctt tacagtcatc ataattcatt ttggcaatca cctcagagaa
     1381 aatctcaatt ttgccggacg gtgtgcctaa tggatttagg agtgggtcat tacgaaattc
     1441 ttcataacga acccattttt tcgctttgtc tgatgcttta aaagaaagtg gtttattctc
     1501 ttgccagaat ttctcgaatt tcggcataat cacacgattt ttgcgtgctg cctcaaatgc
     1561 agtttgatag aaactttgta gccaatccat ttcagatttg ccttcggtaa attccgcttc
     1621 tttgcctgca cgtttggcta gttcggcaaa aatatcgtag tcgttttttg cctcaaattg
     1681 cggttcgacc acctgtttca tcgggaaaat attcatcatt gaataatcgc ccgacatagt
     1741 taagtcatta cgctcatagc tggtggttgc cggcagcacg atgtcagaca tacgagccgt
     1801 tggtgtccaa tggcattcat tgacgataat agtatccggt ttttgccacg ctttcaccaa
     1861 agtgttggtg tcttggtggt gggtaaacgg attaccccct gcccaataga caattttgat
     1921 atcaggataa gtaatttgtg tgccattgta atcaatggtt ttgcccgggt tgagcagtgc
     1981 atcggaaata cgagccaccg ggaatgaaga ttttgatgtt tcatctaacc aggttttcgc
     2041 tccggcttct actgccggat ttgctgtgat agagcctaaa atgccccctg tggcactcgg
     2101 cacgccgccg tttgagtaat ggtagcttaa accgaaacca ccacccggta agccgatttg
     2161 acctaacatt gcagctaggg tgactaacat ccaatgccct tgttcaccgt gtcgttggcg
     2221 ttgaatcccc cagcctgcca ttaacattgt gcgtttagat tggaaatcag tcgctaattt
     2281 tttaatggtt tcttccggca caccacaaat ttgacttgcc cattttgcat ctttcggttg
     2341 attgtctgtt ttaccgagca gatattcttc aaacttctca aaaccggtgg tgtatttttt
     2401 aaggaatgct tgatcgtgtt tgccttcagc gactaaggta tgagccatac cgagcattaa
     2461 cgcaacatca gtgccggtgt ttaccggtac ccattctgca cctaaaaatt cgcaagattc
     2521 actttttacc gggtcaatac agataatgcg tttcccgctc tctttgagtt ttttgaagta
     2581 ttcaatacct tgttgatcgg tagaagtcca cgcaatgcgt agcgtattta acgggttagc
     2641 tgaccaaagc accacaatat ctgaactttc taaaatcact tcccaactag tttgctgttc
     2701 gtacacctcg attgtaccca agacgtgtgg cataatcact tgagcagcac cggtagaata
     2761 gtcgcctttt acaccaacaa atccgccggt aacatttaaa tagcgttgta acagtgtacg
     2821 ggaagcgtgt aatgccccag aactatacca accgtaagaa ccaccaaaaa tagcattcgc
     2881 cccgtgagcc tcacgaacac gtttcatttc attcgcaact aattcaaatg ccttatccca
     2941 cgaaactcgc acccattcat cacgcccacg tagagtagta tctttattac cttctaagta
     3001 gcctttacgc accattgggt attttactcg tgtttcaccg tgtacttggt ctgctaccac
     3061 gtgttgcaat tcatttggca cagaaatctc aaaaacagaa tcagatttca ccactttgcc
     3121 attttctacc actacaccta aaggccccca gtgggcggca gttactactg ttttcatctc
     3181 ggctgctttg gcactggtgg aagctaatgc ccccactaca ccggtagtac cactggctaa
     3241 acctgcaccg gcagctaagc ttaatgatga attttttaga aaaccgcgac gacttacgtc
     3301 aatattgtct tgttttttca taataaacct cgtttgaagt taagttaaaa atagacacct
     3361 ttctaaagtc gagtggataa accatttctc gtgtatccat tggtaaaagc accgacggta
     3421 atttttgcaa aatgttagga aaaaatgacc gcttgtagta ggtgctttac ggcttgctac
     3481 catatctctg ctctcagcag acacttaggc tcacaagctc ggtatcctct aagttgcaat
     3541 taatgggaac tgcctgctac atcttttgca ttacgttgga gatagatagt caccgcacgc
     3601 acatcatcag ccgacataga tgttctgtct ttcattgaat ttaccacccc aatccattga
     3661 tttgcggtgt aatgttctgc cccaatcggt gcatggcaac cgctacagtg tgtttggttt
     3721 aagttatcgc caaaatgatt tagtgcagta atattggcag tgactgcatt tttaggagca
     3781 gtaatttcaa aattgatttc tttccaattt gagttggtga cattatcaaa cactgttttt
     3841 gtgacattca ctttgctttg agcgtcatca ggtagaagag caaccataat acgcttgcct
     3901 aaatccatat acaccacaga ttccgcacct tcttgttgcc agccattaac cgttgcttta
     3961 acctgctcgc cttctgctgt ccattgatgt aattctgcat aaggtaacaa gcgaatttct
     4021 ccaccttgag cgagctgcac tggagtcatt gcgagcgtat aaagggtttt atcatttgtg
     4081 ctaaattccg caccgtgttt agctaattcg cccattgctg cggcattatc taccggcaca
     4141 tcaggcataa aatgggcaat tcctttatgg caatcaatac aggtttgatt agtttcttgg
     4201 gctagtttgt gcattttctg tgccgcttct gtttgttcag aaagtaccat cgcatcaaaa
     4261 ctatgacagc ttttacaggt catagaatcg ttttctttca tttctgccca aacacgttgt
     4321 gccatttcta gacgatgggc ttcataggct tcttgatcag gtaatttatt ggttacaacg
     4381 gtgtgccaaa catcttttaa tgcgatgatt ttagctttga cataatgcaa gccgtcttgt
     4441 ggtagatggc aatccgaaca ctcggctcga attcccttac ggttggaaaa atggacactg
     4501 ccttcccact ctgcctgtgg gtgagtcata gaatggcagc tcacacaaaa ttctgttgag
     4561 ctggtggctt tcattgcata ttgtgtgcca cctaataccg cagcacctaa tgccataata
     4621 ccgacaaata cagcaatttt tttcatctta gccataaatt accccactta atacactaaa
     4681 aattaaagac agaggcattg tattaactcc caagaggtat aaaaataagt aaaaatacct
     4741 attacctcga aagagtgaga ataatttgat ctaaggcaaa aatatgtagt gaaattttag
     4801 gctttttcag agaaccacgg cggtaagata aattgtgcat tggcaaacaa ctccggtgca
     4861 atcacacggc tctgcccttg ttggatttgg taaaccaact gtggcaagct gatagataag
     4921 tcgtgggttt ctgcgtaagt taatccaatt tggctatcgg tattaaaata gtaagtgccg
     4981 tttaccccat aatgtggctg taagcggatc gcattggaga cggctttaaa atgccgtggt
     5041 gaaacactct gtttccacgc atttgccaga atattcgctt ggtcataagc aatactggct
     5101 tgggaataag tgggcgattt gccgaaaaac cgttcgtaat gttggctaaa ctgtctgcct
     5161 aaataatttt cgcttaaccc actattggta ctccaaagaa cgccttcgca aagtttctcg
     5221 tgtggcaaaa acgccgaagg tgcataaata ctgtaaataa tggcatttgt tgggttgcga
     5281 atgaacgttt gataaaactg tacaatatct tctgcaaaat aagaggcaaa cacaataatg
     5341 gaaggtgcga gattatgcac ctgtgccatt aatgtggcat aattatcttg ctcttgggtg
     5401 agttccagat aatccacatt ccagccttgc tcccgcaaac ttagggttaa actttcaata
     5461 ccgatatcaa ctttttgcca actcaccgtt accaccatca ccgttttatt ctgaagccaa
     5521 ggggcataat ggtaacggta atattgcaga aagtgagata tacctaagcc ataggttaca
     5581 tcactggcgc aaacctgaaa catattttca attcgttgat tcggtaaatt ttgggcaagt
     5641 ttatttgagc cactatgggt tgcaatatgc aaataaggaa tgcctttagt tgcaattaat
     5701 tcgtgtactt ccggcaagta gcaggcataa gcggtggaaa tcgcatcaac ttcttgatca
     5761 aataaatgat ggtatgcctg aagaatactt gctttatctt taacataaaa cccagccgtt
     5821 gcaacctgta cttctctgcc gtgaatacca ccttgttgat tgatggtctc aaccgcaagt
     5881 tgagtgccgt taagtagctc tcgggtatcg atttgcccaa taccctgttc aacataaggc
     5941 acgccaataa taatcggacg actatgatgg tggtttatcg taaaaatcgg tggttcgtgt
     6001 cgagttgaag ccgttacaag cggttgtttt ttcgcgataa tttgcaatgc cagttgttca
     6061 atgttaaagc tggcaagaat ggattgggtt agttttcccg gtgtgggtaa acagattaaa
     6121 tgttcggtaa tggcaaaaac agctaataag gttcggcttt tggtatgtgt tttcgcaaaa
     6181 atattttcga tatgtttatc aacagtccgt ttgctgatac aaagctcatt ggcgatatcc
     6241 tcattgctca aaccactgct aattagagtg agaagctcaa tctccctttt actcagtcca
     6301 taaggcaaat cggtatgctc tgcttgagtc aatacaaacg attggtggcg ttctaggctg
     6361 atcttaagcc attgatggtt tgaactatag taaaactgca agctattttt atctgaatag
     6421 agcaatgcct gtgcatagtg gcaaatttgc ggtaattcag caaaatagtc tgcccctgct
     6481 ttgtcttccc attctgatat ttcgccttgt tcatttacta aacaagacca tcggctacgt
     6541 ttatccattc tcacctcgaa ctaaaaacgt tagctaaaat agcaaaatta tctcggcatt
     6601 aatatgatat aaatcaaact aagcaacggt tgccagattt ttataggtag agcatacaat
     6661 ttaaattata cctcttgcct aattaggttt ctcaccataa aatgaaaacc aatttattga
     6721 agactccgag cattatttcc taactctaaa aattaaagac aaatttttga tatggaattt
     6781 atgccagtaa cagcataaag ctgttcaaag gataacttag aaatgggttg tcctctcgtg
     6841 tttagaaagg tgtcaaatgc agtctattat cccaattaaa aacgtgggtg atgatgtcgt
     6901 ttcaggttta gttgatcgct ttcagcgtca atatacttat ttgcgactat ccattactga
     6961 tgtttgtaac ttccgttgta attattgctt gcctgatggc tataagccgc cttcacataa
     7021 gcaaactttt cttagcgttg atgaaatcca gcgggtggct aaagcctttg ccaatttagg
     7081 gacggaaaaa atccgtatca ccggtggtga gccaacttta cgcaaagatt tcctcgaaat
     7141 cgctcacact atttcacaaa cacccggtat ccgtaaagtt gccctaacca ccaacggcta
     7201 tcgcctagag cgtgatgttg aactgtggca acaagccggt attaccgata ttaatgtgag
     7261 tgttgatagc cttgacccac gccaatttca actgattacc ggcgagaaca agttgcaatc
     7321 tattttaaag ggtattgata aagcctttga aattggctac ccaaaaatta aagtcaatgc
     7381 ggtgctaatg aagcaatata ccgcccctga attggataaa ttcctcactt ggatcaaaga
     7441 caagccgatt caaatgcggt ttattgagct gatggaaacc ggtgaaatgg atagtttctt
     7501 ccaagctcaa catctttctg gtcaatcggt gatgacccgc ttattacaag aaggctggca
     7561 attacaaccc aaagcgatgt ctgacggccc ggcaaaagtg ctttctcacc ctgattttaa
     7621 aggcgaaatc ggcttaatta tgccctacga gaaaaatttc tgtgcctctt gcaaccgctt
     7681 gcgtgtttcc gcacttggta aactgcatct ttgcttattt ggtgaagaag ggattgatat
     7741 tcgtgattta ctggcaagcg atgagcaaca gttacagctt gaaacccgcc taaaatcttc
     7801 cttacaaggc aagcgagaac accactattt gcatattggg gatagtgggg ttaggaataa
     7861 tttggcgagt atcggtggtt aaacaaccac cgagcgtgtt ttttctgtta cttttctttg
     7921 cttcgccaaa gaaaagtaac caaaagaaag gcgaccccag attttcctta atccttcgct
     7981 tgttgctaac ttttgggacg aacttttcaa actcgccaac tgcgttggct caaacacgaa
     8041 aagttgtcct caaaagttta cgcaatcgct acggcggaaa ataggggcaa gcggtacatt
     8101 atttaagatt tttgcaagtt tttatttaaa aataaccgct tgtaatgttc cagcttctcc
     8161 cttctgaacc gcctgagcga ctttgaattt ataggaaaaa tttagtgttt gagccaacga
     8221 agttggcgag ttttaaattt
//
LOCUS       DANLXF010000099         8182 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.099, whole genome shotgun sequence.
ACCESSION   DANLXF010000099 DANLXF010000000
VERSION     DANLXF010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8182
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.099"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            135..371
                     /locus_tag="PXD66_001458"
     CDS             135..371
                     /locus_tag="PXD66_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HDL6319751.1"
                     /translation="MSRTTSVTLAEPLSQFVNQMVESGRYGSTNEVISAALKILEAQE
                     QQLIQLRALIDEGLESGVSEETVQSIISKAKRNV"
     gene            610..2628
                     /gene="uvrB"
                     /locus_tag="PXD66_001459"
     CDS             610..2628
                     /gene="uvrB"
                     /locus_tag="PXD66_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HDL6319752.1"
                     /translation="MSKQGKPFILHSPFKPSGDQPSAIAKLTEGLNDGLAHQTLLGVT
                     GSGKTFTIANVIAKLNRPAMVLAPNKTLAAQLYAEMKAFFPENAVEYFVSYYDYYQPE
                     AYVPASDTFIEKDASINEQIEQMRLSATKSFLERRDTIVVASVSAIYGLGDVDAYMQM
                     MLHLQVGAMIGQREILSRLAELQYTRNDQAFQRSTFRVRGDVIDIYPAESDEVALRVE
                     LFDDEIENLSLFDPLTGHSLGKVPRYTIYPKTHYVTPRERILEAIEQIKVELADRRTY
                     FIKENKLLEEQRIAQRTQFDIEMMNELGYCSGIENYSRYLSGRKEGEAPPTLFDYMPA
                     DGILVIDESHVTVPQIGGMYRGDRARKETLVQYGFRLPSALDNRPLRFEEFERLSPQT
                     IYVSATPGAYELEKNPDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIHKRVAVDER
                     VLVTTLTKKMAEDLTDYLDEHGVRVRYLHSDIDTVERVEIIHDLRMGMFDVLVGINLL
                     REGLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNLNGKAILYGDRITNSMQKA
                     ITETERRREKQMKYNEEHGITPQALNKKVGELLDIGQSDKPKRGKQAVKKAENSATDY
                     KPKSRKELEKELKTLEQQMRDFAKDLEFEKAAAVRDKLQHLKAYLLEV"
     gene            2631..3041
                     /locus_tag="PXD66_001460"
     CDS             2631..3041
                     /locus_tag="PXD66_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319753.1"
                     /translation="MISRSLFSKLILFAKIVIFSQLVFMVVIWIAHHIMREYSNPFER
                     FGTLFNNVFLTFIIPMMVSSAVIVYKGYTQKNEDKVFWIYMGFFLIWILGFIYFEPAV
                     YDKSLKISGAGFGAMLSIIICSKLLPKETNKEQL"
     gene            complement(3262..4350)
                     /gene="purK"
                     /locus_tag="PXD66_001461"
     CDS             complement(3262..4350)
                     /gene="purK"
                     /locus_tag="PXD66_001461"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDL6319754.1"
                     /translation="MQKSAIYPPVYVLGNGQLGRMLRYAGAPLDIEVKPLAFDAPVFD
                     IEPNSIITAEIERWADTPLTQLLGNHPNFVNLNVFGKLADRFTQKSLLDELHLATSKW
                     QLLENAEHWQDIFQHIGEKVVVKRRTGGYDGRGQWIVTQDSIHQITPELFGEVIAEKF
                     IPFEGEISIVGARFRDGSTRFYPISHNLQQNGILRYSVSDVNLPNQALYQQQAEQMLA
                     TVMQKLEYVGVMAMECFVVGDKLLINELAPRVHNSGHWTQLGCAISQFELHLRALLDL
                     PTPALDQIAPSVMVNLIGIEHNKQWLSLPFSQLHWYGKEVRAGRKVGHINLTHTDKNG
                     LIELLEQLRPSLTEDFHSGLDWAINKLK"
     gene            complement(4369..5334)
                     /locus_tag="PXD66_001462"
     CDS             complement(4369..5334)
                     /locus_tag="PXD66_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDL6319755.1"
                     /translation="MSKKLLILHTGGTISMKEGEDGKVSPSNENPLLAALERLNHPAK
                     LAQESLFNIPSPHINIEHWQQLKMRIEKAVNEENVDGVVITHGTDTLEETSYFLDLAL
                     SVNIPVVITGAMRSSNELGADGLINLQSAILVALNEESRDKGVLVVMNDEIHNAKFVT
                     KTHTTNVATFQTPTFGPCGLVTKESVIFFQKLTTYERYPIQQVTKANVQLLKAYAGMD
                     SFLLEQLAKSKCDGVVIEALGAGNLPPSCLAGIEALVNANIPIVIVSRAFNGITQDVY
                     DYVGGGKQLKQKGIIFTKGLSGQKARIKLMVLLSQTLDKPLSDYF"
     gene            complement(5349..5846)
                     /gene="purE"
                     /locus_tag="PXD66_001463"
     CDS             complement(5349..5846)
                     /gene="purE"
                     /locus_tag="PXD66_001463"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDL6319756.1"
                     /translation="MPNKAEIAIVMGSKSDWATMSEATQILDQFGLSYHVEVVSAHRT
                     PDKLFSFAETAETNGYKVIIAGAGGAAHLPGMIAAKTIVPVLGVPVKSSMLSGVDSLY
                     SIVQMPKGIPVGTLAIGPAGAANAGLLAAQILAAFNPDIAEKLRIFRQTQTQMVLDNP
                     DPRVE"
     gene            6020..6664
                     /gene="sspA"
                     /locus_tag="PXD66_001464"
     CDS             6020..6664
                     /gene="sspA"
                     /locus_tag="PXD66_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HDL6319757.1"
                     /translation="MTSSINKRSVMTLFSDKNDIYSHQVRIVLAEKGVPYEIENVMLG
                     SISEDLLELNPYGNIPTLVDRELVLFNPRIIMEYLDERFPHPPLMPVYPVSRGQCRLN
                     MYRIEQDWYSQIDIVNKDPESAEGKKALAQLKEDVLALAPIFAAKPYFMSDDFSLVDC
                     YIAPLLWRMQILGVKFSGATAKPINAYMTRVFQRDSFIQSVGGEAPKHLMDDKD"
     gene            6664..7071
                     /locus_tag="PXD66_001465"
     CDS             6664..7071
                     /locus_tag="PXD66_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HDL6319758.1"
                     /translation="MKPLRPYLYNAYYNWIIDSDNTPYLLVNAEFPDVDVPVEFVKEG
                     KIILNISPRSIGQYIVADEYISFSARFQGMLRDIYIPFGAMVAIYAQETGDGIMFQEE
                     AYYTEEAYQARVRAVEQPKKIKAKKTSTLKLVK"
     gene            7268..>8182
                     /locus_tag="PXD66_001466"
     CDS             7268..>8182
                     /locus_tag="PXD66_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL6319759.1"
                     /translation="MKFHKTLLVTLLSSLSVAALAQDKIQNVAITAIVEHPALDSIRK
                     GVIEELAREGFVDGKNIKIDYQSAQGSTATAAQIARKFVGDKADIIIPITTPSAQPVV
                     AATRSIPIVFSGVTDPVAAKLVKSWEPSGTNVTGISDHKAMAPQVKLIQTLVPDLKAV
                     GYVYSAGEVNSAIVLEELKQEAQKQGFTIVPVAVQRSADIGTAARSLNGKVQAIYISE
                     DNAVVSAYEALHKAALEAKIPVIAADRDTVQRGAVAAYAVNQYDIGVATGKVAARVLK
                     GEKAGTIPIQEVSKLELSINTKTAKELGI"
BASE COUNT         2348 a         1625 c         1832 g         2377 t
ORIGIN      
        1 ttcgcaaaaa ataatgctga ttttgtgatg taaatcacat tttgttttgc aaattattgc
       61 taaaaataga ccgcttgctt atctaaatta gagccattac aatctgctca ataatcaata
      121 agataggaat agtaatgagt agaacaacca gcgtaacttt agctgagcca ttaagccaat
      181 ttgtgaacca aatggttgaa agtgggcgtt atggttcgac caacgaggtc atcagtgccg
      241 cattaaaaat attggaagca caagaacagc aactaattca gctaagagcc ttaattgatg
      301 aaggcttaga aagcggtgtt tctgaggaaa ccgttcaatc tattatcagc aaggcaaaac
      361 gtaatgtata aactttctgt tagggcagag cgagatttag cgttacttta ttcgcccttt
      421 gttacgctgt cattaccacc gctcacattc tatttattat aaaccaatcc caaaaggtat
      481 ctttattgtt agggtgttag atccaagaat ggatcaatat aagcagttac aataaatttg
      541 caaaaatccc gataaaaacg accgcttgct tgtgctaaaa taaaccgatt ttttaagaca
      601 ggattttcta tgagcaagca aggcaaaccc tttatcctcc attctccttt caagccctcc
      661 ggcgaccaac caagtgcgat tgccaaattg accgaaggct taaatgacgg cttggcacat
      721 caaaccttac ttggcgtaac cggctcgggg aaaaccttta ccattgctaa tgtgattgcc
      781 aagctcaatc gcccggcgat ggtgcttgcc ccgaataaaa cccttgccgc ccagctttat
      841 gccgagatga aagcgttctt tccggaaaat gcggtggagt atttcgtctc ttactatgat
      901 tactaccagc ccgaagccta tgtgccggca agcgatacct ttatcgagaa agatgcctct
      961 atcaatgagc agatcgagca aatgcgactt tccgcaacca aatccttttt ggaacgccga
     1021 gatacgattg tagtggcgtc cgtttctgcc atttacggct tgggtgatgt ggacgcttat
     1081 atgcagatga tgttgcattt gcaagtgggg gcgatgattg ggcagcgtga aattctctct
     1141 cgccttgccg aattgcaata tactcgcaac gatcaggcgt tccaacgttc gactttccga
     1201 gtgcggggcg atgtgattga tatttaccct gccgaaagcg atgaagtagc gttaagggta
     1261 gagctgtttg acgatgaaat cgaaaatctg tcgctgtttg atccgcttac agggcatagc
     1321 ttgggcaagg tgcctcgcta taccatttac ccgaaaactc actatgtgac ccctcgggaa
     1381 cggattttgg aggcaatcga gcaaattaag gtcgagcttg ccgaccgtcg cacctatttc
     1441 atcaaagaga ataaattgct ggaagagcag cggatcgccc aacgtacgca gtttgatatt
     1501 gagatgatga acgagctggg ctactgttcg ggcattgaaa actactcacg ctatctctcg
     1561 ggcagaaaag agggcgaagc ccctccgacc ctgtttgatt atatgccggc ggacggtatt
     1621 ttggtgattg atgaaagcca cgttactgtg ccacaaattg gcggaatgta tcgaggcgac
     1681 cgagcgagaa aagaaacatt ggtgcaatac ggtttccgtc tgccgtctgc actggataac
     1741 cgcccgttga gatttgaaga gtttgaacgt ctttcaccac agacgattta cgtttctgcc
     1801 acaccggggg cgtatgaatt ggagaaaaat cccgatgtgg tggatcaagt agttcgtcca
     1861 accggcttgc tcgatccgat tatcgaagtt cgccccgttg ctacgcaggt ggacgattta
     1921 ctgtctgaaa ttcacaaacg agtggcggtg gacgaacggg tgctggtgac aacactcacc
     1981 aaaaaaatgg cggaagatct caccgattat ttggacgaac atggcgtacg agttcgttat
     2041 ctacatagcg acattgacac ggtggaacgg gtcgaaatta tccacgattt acgaatggga
     2101 atgtttgatg tgctggtggg gatcaactta ttgcgtgagg gcttagatat gcccgaagtg
     2161 tcgctggttg ccattcttga tgcggataaa gagggatttt tacgttccga acgctcgtta
     2221 attcagacca tcggacgtgc cgcccgaaac ctcaacggaa aagcgatttt atacggcgac
     2281 cgcattacca actcaatgca aaaagcaatt actgagaccg aacgccgccg tgaaaagcag
     2341 atgaaataca acgaagaaca cggcatcacg ccacaagcgt tgaacaaaaa agtgggtgaa
     2401 ttgctcgaca tcggacaatc cgacaagcca aaacggggca aacaagcggt caaaaaagcc
     2461 gaaaattctg caaccgacta caagccgaaa tcccgtaaag aactggaaaa agagcttaaa
     2521 acgctggaac aacaaatgcg tgattttgcg aaagatctgg aatttgaaaa agcggctgcg
     2581 gtaagggata agttgcagca tttaaaggcg tatttgttgg aggtttaaat atgatttctc
     2641 gttcattatt ttcaaaatta atcttatttg ccaaaattgt tattttctct caacttgttt
     2701 ttatggtggt tatctggatt gcccaccata ttatgcggga gtacagtaat ccgtttgaac
     2761 gatttggtac cctgttcaat aatgtgtttt tgacttttat tattccgatg atggtaagta
     2821 gtgcggttat tgtttataaa ggttataccc agaaaaatga agataaagtg ttttggattt
     2881 atatggggtt ctttttaatt tggattttgg gatttattta ttttgagccg gcagtttatg
     2941 ataaatcact gaagatttca ggtgcaggat ttggggctat gctctctatt atcatttgct
     3001 cgaagttatt gcctaaggaa acaaataaag agcagctcta agattcttgt gaagaaatct
     3061 gttttctcgg aggaagaaaa tgctttttga tagccatatc caccctgagg ggttttcaga
     3121 tcaagatgat gaccggaagt agccattatg ttgaattatt ttgcaaattt tagcactaag
     3181 tcgaccgctt gtttggtacg agcgaggaag ctcgcaccat cgggtaaaca gatttgcaaa
     3241 aaagttacaa aatccgaccg cttacttgag cttattgatt gcccaatcta atcccgaatg
     3301 gaaatcttcc gttaggctcg gtctgagttg ttccagtagt tcaattaagc cgtttttatc
     3361 ggtgtgagtg aggttgatat gcccaacttt tctaccagct cggacttctt tgccatacca
     3421 atgtagttgg ctaaatggga ggcttagcca ctgcttgtta tgttcaatgc ctattaaatt
     3481 aaccatcacg cttggggcaa tttggtcgag tgctggcgta ggtagatcta ataatgcccg
     3541 taaatgtaac tcaaattggc taatggcaca gccgagttgt gtccagtgtc cgctgttatg
     3601 cactcgaggg gcaagctcgt tgatgagtag cttgtcgccc acaacaaagc attccattgc
     3661 cattacgccg acatactcta atttttgcat taccgtagcg agcatctgtt cggcttgctg
     3721 ctggtagagg gcttggttcg gtaaattcac atcggagacg gagtaacgta gaatgccgtt
     3781 ttgttgtaaa ttgtgagaaa tagggtagaa acgggttgag ccgtcacgga atctcgcccc
     3841 aacaatggaa atttccccct caaacgggat aaatttttcg gcaatcactt cgccaaataa
     3901 ttcaggtgta atttgatgaa tcgagtcttg cgttacaatc cattgcccac gtccgtcata
     3961 tcctccggtt cggcgtttaa ccactacttt ttcgccaata tgctgaaaaa tatcttgcca
     4021 atgttcggca ttttctagca gttgccattt tgaagtcgca agatggagtt catcaagtag
     4081 tgatttttgc gtaaaacgat cggctaactt cccaaaaaca ttcaggttca caaaattcgg
     4141 gtggttaccg agtaattgcg ttaaaggcgt atctgcccaa cgttcgattt ctgcggtaat
     4201 aatgctgttt ggctcaatat caaacacagg ggcatcgaaa gccagcggtt tgacttcaat
     4261 atcaagtggt gctccggcgt agcgtagcat tctcccgagc tggccgttgc ctaatacata
     4321 cacaggtggg tagattgcac ttttttgcat cgtggtattc tcaatgggtt agaaatagtc
     4381 tgatagtggt ttgtctaagg tttggctgag taagaccata agtttaatgc gagctttttg
     4441 cccacttaag cctttggtaa aaataatgcc tttttgcttt aactgttttc caccgcctac
     4501 ataatcatag acatcttggg taataccatt gaatgcccga gaaacaatta caattggaat
     4561 attggcattc actaaagcct caataccagc cagacaacta gggggtaaat tacctgcacc
     4621 caatgcctca atcacaacac catcgcattt gcttttcgct aattgttcca gcagaaaact
     4681 atccattccg gcgtatgctt tcagtagttg cacgttagct ttagttactt gttgaattgg
     4741 ataacgttcg taagtggtca atttttggaa aaaaatgaca gattctttcg ttactaagcc
     4801 acaaggccca aaggttgggg tttggaaagt cgccacatta gtggtgtggg ttttggtcac
     4861 aaatttagca ttgtggattt catcattcat caccactaac acgcctttat ctcggctttc
     4921 ttcatttaac gccactaaaa tcgcactttg tagattgatt aagccatcag cacccagttc
     4981 gttactggaa cgcattgctc cggtaattac taccggtata tttacgctga gggctaaatc
     5041 cagaaaatag gaggtttctt ccaaggtatc tgtgccgtgg gtgattacta cgccatcgac
     5101 attctcctca ttcactgcct tttcaatccg catttttaac tgttgccaat gctcgatatt
     5161 gatatgcgga gagggaatat taaataacga ttcttgcgcc aattttgctg ggtggtttaa
     5221 acgttctaag gcagccaaga gaggattttc attggaaggg gagaccttac cgtcttcccc
     5281 ttctttcatt gaaatagtac caccagtgtg gaggattaat aattttttag acatctttta
     5341 tacaatagtt attcaacacg aggatcagga ttatctaata ccatctgagt ttgggtttga
     5401 cggaaaatac gcagtttctc tgcaatatca gggttaaagg ctgctaaaat ttgagcggct
     5461 aataagcctg catttgctgc acctgccggc ccgattgcga gcgtaccgac cggaatacct
     5521 ttcggcattt gcacaatcga atagaggcta tctacaccac ttaacattga gctttttacc
     5581 ggcacaccta acaccggcac aatggtttta gcagcgatca ttccgggcaa gtgagctgca
     5641 ccgccggctc cggcaataat cactttatag ccgttagtct ctgcagtttc agcaaaagaa
     5701 aagagtttgt ccggtgtgcg gtgtgctgag acgacttcaa cgtgatagct caaaccgaac
     5761 tggtctaata tttgggttgc ttcagacatt gtagcccaat cactcttaga tcccattacg
     5821 atcgcaattt cagctttatt tggcatttta aatccttatg atactaatgt aaaacagtgt
     5881 aatcatagca tacaaagcaa acgtttgcgt agctatttaa gaagtgaaaa gcaattttta
     5941 gggctgttat ctgctggtta aaatatgata taatcgacaa ccattttatt ttttataaat
     6001 ttaatcatcg gagatataaa tgacgagtag tataaacaaa cgttcggtaa tgactctttt
     6061 ttcggataaa aatgatattt acagccacca agtgcgtatt gtgttagcag aaaaaggggt
     6121 gccttatgaa atagagaacg ttatgctagg cagtatttct gaagatttat tagaattgaa
     6181 tccttacggt aatattccta cattggttga tcgtgaatta gtgctgttta accctcgtat
     6241 tattatggaa tatttagatg agcgtttccc tcacccacca ttaatgccgg tttaccctgt
     6301 ttctcgtggt cagtgccgtt taaatatgta tcgtattgaa caagattggt attcgcaaat
     6361 tgatattgtg aataaagatc ctgaatcagc ggaaggcaag aaagcgttag cacagcttaa
     6421 agaagatgta ttggcattag cgcctatttt tgccgctaaa ccttatttta tgagcgatga
     6481 tttcagcttg gttgattgct atattgcacc gttattatgg cgtatgcaaa tcttaggcgt
     6541 taaattcagc ggtgcaacgg caaaaccaat taatgcgtat atgacccgtg tattccaacg
     6601 tgatagtttt atccagtctg ttggcggcga agcgccaaaa catttaatgg acgataaaga
     6661 ctaatgaaac cattaagacc ttatctttat aatgcctact ataactggat tattgatagc
     6721 gataacacgc cttacttatt agtgaatgca gaatttccag acgtagatgt gccggtagag
     6781 tttgtcaaag aagggaagat tattttaaat atttcgcctc gttccatcgg gcagtatatt
     6841 gtggctgatg aatacattag ctttagcgct cgttttcagg gaatgttaag agatatttat
     6901 attccttttg gggcaatggt ggcgatttat gcgcaagaaa ccggtgatgg tattatgttc
     6961 caagaagaag cctactacac tgaagaggct tatcaagctc gagttagagc agtagagcag
     7021 cctaaaaaaa tcaaggcgaa gaaaacatct accttaaagt tggtgaaata agagatctat
     7081 ttcatcaaaa cacattacaa gcggttaaat ttttctgatt atttgcaaat atcagtcaaa
     7141 atttaaccgc ttgtttgttt tgtgacggaa ataatcaaat tccacttgca ctccgactaa
     7201 aaattgctta tctttgtttt gtgtttgtac tgttgtgata gtacagtaat gatcttaagg
     7261 agagaaaatg aaattccata aaaccttatt agtgactttg ttaagttcac tttctgtagc
     7321 ggcattagct caagataaaa ttcaaaatgt agcaattaca gcgattgtgg aacaccctgc
     7381 attagattca attcgtaaag gtgtgattga agaattagcc cgagaagggt ttgttgatgg
     7441 taaaaacatt aaaatcgact atcagtctgc acagggtagt actgcaacag cagcacaaat
     7501 tgcgcgtaaa tttgttgggg ataaagcaga tattattatc ccgatcacca caccttcggc
     7561 acaacctgtt gttgctgcaa cacgttctat cccaattgtg ttttcaggcg tgacagaccc
     7621 ggttgctgca aaattagtaa aatcgtggga gccaagtggc actaacgtga cggggatttc
     7681 agaccacaaa gcgatggctc cacaagtgaa attaattcaa acattagtac cggatttgaa
     7741 agctgttggt tatgtgtata gtgcaggtga agttaattct gcgattgtgt tagaagagtt
     7801 aaaacaagag gctcaaaaac agggctttac tatagtgccg gttgctgttc aacgcagtgc
     7861 cgatattggc actgctgctc gcagcctaaa cggaaaagtg caggctattt atatttctga
     7921 agataatgca gttgtttcgg cttatgaagc cttacataaa gcggctttag aagcaaaaat
     7981 tccggtgatt gctgctgacc gtgatacagt acaacgtggt gcagtggcag cttatgcggt
     8041 taatcaatat gatattggtg tggcaaccgg taaagttgct gctcgtgtat taaaagggga
     8101 aaaagcaggt acaattccaa tccaagaagt cagcaaatta gagctttcaa ttaatactaa
     8161 aacggcaaaa gaattgggca tt
//
LOCUS       DANLXF010000100         8175 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.100, whole genome shotgun sequence.
ACCESSION   DANLXF010000100 DANLXF010000000
VERSION     DANLXF010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8175
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.100"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(350..2020)
                     /gene="glnS"
                     /locus_tag="PXD66_001467"
     CDS             complement(350..2020)
                     /gene="glnS"
                     /locus_tag="PXD66_001467"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HDL6319760.1"
                     /translation="MSEEILTAETGTRANFITHIIDEDLASGKHNNVYTRFPPEPNGY
                     LHIGHAKSICLNFGIAQDYNGLCNLRFDDTNPVKEDVEYVDSIKQDVEWLGFKWEGEP
                     RYASDYFDQLYGYAIELINKGLAYVDELSPDEMREYRGTLTEPGKNSPYRDRSVEENL
                     ALFEKMKNGEFAEGKACLRAKIDMASPFIVMRDPVLYRVKFAHHHQTGDKWCIYPMYD
                     FTHCISDAIERITHSLCTLEFQDNRRLYDWVLDNISIERPLPHQYEFSRLNLEGTLTS
                     KRKLLKLVTDGIVDGWNDPRMPTISGLRRRGYTPASLREFCRRIGVTKQDNVVEYSAL
                     EACIREDLNENAPRAMAVINPVRVVIENFGEKEILKAPNHPNRPELGERDLPFTREIY
                     IDEADFREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDASGRISTIFCTYDPDTLGK
                     NPADGRKVKGVIHWVSAEDNKPAEFRIYDRLFTVPNPGAEEDINAVLNPESLVVKHGF
                     VESSLAHAKPEQDYQFEREGYYCLDSKDGSADNLVFNLTVSLKESVAF"
     gene            complement(2181..2624)
                     /locus_tag="PXD66_001468"
     CDS             complement(2181..2624)
                     /locus_tag="PXD66_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HDL6319761.1"
                     /translation="MNLQQIQDRLALKDLVDTFSNLADEKKVAEQMPLFTQDAVVNTY
                     IGGKLVFEMHGVAEIEQVFTTFLTPFHSVYHLNGQHTVPFIDENNATAINYCAVKLVE
                     TKEGKEILHDHSVRYADTYVKQEGKWRIAKRIANFMISESREIRQ"
     gene            complement(2675..3250)
                     /locus_tag="PXD66_001469"
     CDS             complement(2675..3250)
                     /locus_tag="PXD66_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDL6319762.1"
                     /translation="MKQILILGASGSLANVVIPELLKDNNVQLTLFARRPQSVAKFET
                     ERVKIVQGDVLDFHALTQAMQGVDAVYAGLAGDLKAMAGNVVNAMNEAQVKRLVWISS
                     MGIYGETGEDHGAILEPYRQSAAVIEASELDYTVVRPGWFTNGSEVQYQLTHKGEPFK
                     GHSVSRKSIADFVARILRENSEIRESVGIAA"
     gene            3357..4247
                     /locus_tag="PXD66_001470"
     CDS             3357..4247
                     /locus_tag="PXD66_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL6319763.1"
                     /translation="MKENLNDLRAFLLVAQTSSFTKAAAQMGVSQSALSHSIRGIEER
                     LQIKLFHRTTRSISTTEAGERLYQRLLPLFDEIDQEVNGLSEFRNALKGGLRINGNEH
                     VFRDVLADKFAEFVKRYPEVALELVAEDRFVDIVAERFDAGIRLGADVAKDMIALRIS
                     PDVKMTAAASPNYLAQHGTPKTPFDLTEHHCLKHRLATLGGILAWEFRDPITKKIVKF
                     QPQGNLTANNGFLLQHYAKQGLGILWSPQDSIQTELDNGSLVPILTDWAMQYEGYHLY
                     YPNRRQNSPLLKALIDVLRG"
     gene            complement(4314..4880)
                     /gene="efp"
                     /locus_tag="PXD66_001471"
     CDS             complement(4314..4880)
                     /gene="efp"
                     /locus_tag="PXD66_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDL6319764.1"
                     /translation="MASYSTNDFKKGLKFIQDGEPCVIVENEFVKPGKGQAFTRTKIR
                     KLISGKVLEINFKSGTSVEAADVVDSNYNYSYSDGDFWYFMHPETFEQISVDEKALGD
                     NVKWLVDNAECIVTLWNGSAIAVTPPNFVELEIVETDPGLKGDTAGTGGKPATLSTGA
                     VVNVPLFVQIGEVIRVDTRSGEYVSRVK"
     gene            5047..5616
                     /locus_tag="PXD66_001472"
     CDS             5047..5616
                     /locus_tag="PXD66_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319765.1"
                     /translation="MKKLITLSAILAVSVATVAEAKRGGGSFRSVSPSKAVATKPVAS
                     QKSTTNQQTQQKDATFANTPNSATAQPQVRQGSTMANVAMGAAAGYILGDMMSSNAHA
                     NQPSQPADANAQAVNSAQNVANGAVPAIAEFKSIGGQIDPFLVEKTDGYRRYCISGVQ
                     YLVAAQGVQTSPTVMVNPNGTPLQCNLIP"
     gene            5670..6662
                     /gene="epmB"
                     /locus_tag="PXD66_001473"
     CDS             5670..6662
                     /gene="epmB"
                     /locus_tag="PXD66_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HDL6319766.1"
                     /translation="MQPIQIKPLWLTELAQAFNNPIDLLQFLELNPNDFEGDIAARKL
                     FALRVPRMFAEKMEKGNPNDPLFLQAMSLQAEFIEAEGFVVDPLEEQQSPAPNILHKY
                     HNRLLFMLKNSCAINCRYCFRRHFPYEEVKSGKAVWQQGLTYIAEHPELEEVILSGGD
                     PLMAKDQDLDWILTQLEQISHIKTLRIHSRLPVVIPNRITTELCERLSKSRLNVVLVT
                     HINHANEIDAVFANKMAQLKKAGVVLLNQSVMLKGVNDNAQTLKRLSDKLFEYGILPY
                     YLHLFDKVAGASHFYIEDEQAGEIYRELQRITSGYLVPKLAREIAKEPNKILKG"
     gene            complement(6762..8165)
                     /gene="asnS"
                     /locus_tag="PXD66_001474"
     CDS             complement(6762..8165)
                     /gene="asnS"
                     /locus_tag="PXD66_001474"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDL6319767.1"
                     /translation="MSKFPTVSEILSGKIAVGEEATVRGWVRTRRDSKAGLSFLSVYD
                     GSCFDPIQAIINNDLPNYNAEVLHLTAGCSVVVTGKVVESPAEGQAVELQASKVEVLG
                     WVEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHCLAQAIHRFFHEQGFYW
                     VATPVITASDTEGAGEMFRVSTLDLENLPRGENGKVDFNQDFFGKESFLTVSGQLNAE
                     TYACALSKVYTFGPTFRAENSNTTRHLAEFWMMEPEVAFADLNDNAKLAEDMLKYVFK
                     AVLAERKDDMEFFVKHVDKEAISRLENFINSPFAQVDYTDAIEILLKSGKEFEFPVSW
                     GIDLSSEHERFLAEEHFKSPVVVKNYPKDIKAFYMRLNDDGKTVAAMDVLAPGIGEII
                     GGSQREERLEVLDQRMNEMGLNPEDYWWYRDLRKYGTVPHSGFGLGFERLIVYVTGLQ
                     NIRDVIPFPRAPRNANF"
BASE COUNT         2334 a         1770 c         1703 g         2368 t
ORIGIN      
        1 atgctatcat aaaagagcat cacttttggt ttccaaagat tatagaaagc tcaaataata
       61 cactacctca taatttaaaa tgaaacgttt caattggaaa aagtggattt tgtgactgag
      121 atcacaaatt taaacattgg tctgtatttt tgagagattt tgtttgaaac tgccggcaaa
      181 ttcgcttaac cttaaggtaa caagaataag tgttgtacct agcaacctaa cacataaatt
      241 tgctttccga gtggccccaa gaattggggc tttttttatg cttgatgagt cgttttgaga
      301 gtaaaaaaat accagcatat ttgctggtat ttgaatgaag agagttagat tagaacgcta
      361 cgctttcttt taagctgaca gttaagttaa atacaaggtt gtctgccgaa ccatctttgc
      421 tgtctaagca gtagtaacct tcacgctcga attggtagtc ttgttccggt ttggcgtgag
      481 ctaaacttga ctcaacaaaa ccatgcttaa ccactaatga ttcaggattt agcaccgcat
      541 tgatgtcttc ttccgccccc gggtttggta ctgtaaatag gcggtcgtaa atgcggaact
      601 ctgccggttt gttatcttca gcagataccc aatgaatcac gccttttact ttgcgaccat
      661 cagccggatt tttgcccaat gtatccgggt cgtatgtgca aaaaatagtt gaaattcgac
      721 cgcttgcatc tttttccaca cgctcagctt taatcacata ggcattgcgt aaacgaactt
      781 ctttgcctaa gactaaacgt ttatattgct tattggcttc ttcacggaag tcggcttcat
      841 caatataaat ctcacgggtg aacggtaaat cacgctcgcc aagctctgga cgatttgggt
      901 gattaggggc ttttaatatt tccttttcac cgaagttttc aatcaccact cgaaccgggt
      961 taatcaccgc catcgcgcgt ggtgcgtttt catttaagtc ttcacgaatg cacgcttcaa
     1021 gggcagagta ttccaccacg ttatcttgct tggttacccc aatacggcga caaaattcac
     1081 gtaaagacgc tggggtataa ccacggcggc gtaagccgga aatggttggc atacgagggt
     1141 cattccaacc atcaacaatg ccgtctgtga ctaattttaa taatttacgc ttagaggtta
     1201 aagtaccttc taaatttaaa cgtgaaaatt cgtattggtg cggtaacggt ctttcaatcg
     1261 aaatattgtc cagcacccaa tcatataagc ggcggttatc ttggaactct agcgtacata
     1321 gcgagtgagt gattcgctca atcgcatcgg aaatgcagtg ggtaaaatcg tacatcgggt
     1381 agatgcacca tttatcaccg gtttgatgat ggtgagcaaa tttcacacgg taaagcaccg
     1441 gatctcgcat cacaataaac ggggacgcca tatcaatttt tgctctcaag caggctttcc
     1501 cttccgcaaa ctctccgttt ttcatttttt cgaataacgc taagttttct tccacgctac
     1561 gatcacggta agggctgttt ttgcccggct cggttaaagt accacggtat tcacgcattt
     1621 catcaggcga taattcatca acataggcta atcctttgtt gattaactca atcgcatagc
     1681 cataaagctg gtcaaaatag tctgaggcat aacgaggctc accctcccat ttaaaaccta
     1741 gccactccac atcttgctta attgaatcta catattccac gtcttcttta accgggttgg
     1801 tatcatcaaa acgcaagtta cataaaccgt tataatcttg agcgatacca aaatttaaac
     1861 agatagattt agcgtgtccg atatgcaaat aaccgtttgg ttcaggtgga aaacgagtgt
     1921 aaacattgtt gtgctttccg cttgctaaat cttcatcaat aatatgggta ataaaattgg
     1981 ctcgagtgcc agtttctgcc gttaaaattt cttcgctcat agttctctct aaatgtatca
     2041 ataaaaataa cggattattc tacacttaat tgaaacagac ttaaagacta aagcaaaatt
     2101 cctcggtttc ctccaaagaa ttttgctgtt ttagtaagat gcaatggaat tgcaaatttt
     2161 tagctaaatt taaccgcttg ttattgcctg atttcacggc tttcgctgat cataaaattc
     2221 gcaatccgtt tggcaatccg ccatttaccc tcttgtttaa cataggtatc ggcataacgc
     2281 acgctgtgat cgtgcaagat ctctttgccc tctttggttt ctaccagttt caccgcacaa
     2341 tagttgatcg ctgtggcgtt attttcatca ataaaaggca cggtatgctg accgtttaaa
     2401 tgataaacgc tgtgaaacgg ggttaaaaag gtggtaaaca cctgttcgat ttctgccacg
     2461 ccgtgcattt caaacaccaa ttttccgccg atgtaggtgt ttacgacggc atcttgcgtg
     2521 aaaagcggca tctgctcggc aacctttttt tcatcggcaa ggttagagaa tgtatctact
     2581 aaatctttta gtgctaaacg atcttggatt tgttgtaagt tcattgtgtt ttccttctga
     2641 tttttgcaaa ttttctttgg tttttcaccg cttgttaggc tgcaatacca acactttcac
     2701 ggatttcgct gttttcacgc aaaatgcgag ccacaaaatc ggcaatggat ttgcgggaaa
     2761 cgctgtgtcc tttgaacggt tcgcctttgt gagtcagctg atattgcact tccgaaccgt
     2821 tggtaaacca gcccgggcgg acaacggtat aatccaactc gctggcttca atcacggctg
     2881 ccgattggcg gtaaggctct aaaattgccc cgtgatcttc gccggtttcg ccataaatcc
     2941 ccattgagct aatccaaacc aagcgtttta cctgagcttc attcattgcg ttcaccacat
     3001 ttcccgccat cgcttttaag tcgccggcaa gtccagcgta aacggcatca acgccttgca
     3061 ttgcttgggt taatgcgtgg aaatccaaca catcgccttg cacaattttg acgcgttcag
     3121 tttcaaattt cgctactgat tgcggacgac gggcaaaaag ggttaattgc acattattgt
     3181 cttttagcag ttcaggaatc acgacattcg ctaggctacc gcttgctcct aaaattaaga
     3241 tctgtttcat tgctgttctc cattgaatga agtcagtata gcggtgtaga ttgcatttga
     3301 ttagtagggt aaaatcgctt gaagtaatga gaaaaattca ttaataaggt ggttaaatga
     3361 aagaaaacct caacgattta agagcttttt tgttggtagc acaaacgagc agttttacca
     3421 aagcagcagc acagatgggc gtgtcgcagt cggcgttgtc gcacagcatt cgggggattg
     3481 aagaacggtt gcaaatcaag ctgtttcatc gcaccactcg tagcatttca accaccgagg
     3541 caggggagag gctgtatcaa cggctgttac cgctgtttga tgaaatcgac caagaagtaa
     3601 atgggctgag tgagtttcgc aatgcgttaa aaggcgggct gagaattaac ggtaatgaac
     3661 acgttttccg tgatgtgctg gcagataaat tcgctgagtt tgtcaaacgt taccctgaag
     3721 tagcattgga gctagtggcg gaagatcgct ttgtagatat tgtcgccgag cgttttgatg
     3781 ccggtattcg gcttggggca gatgtggcga aagatatgat tgcactacgc atttcgcccg
     3841 atgtgaaaat gaccgcagct gccagcccca actatttggc acagcacggc acgccgaaaa
     3901 cgccctttga tttaaccgaa catcattgtc tcaaacatcg tcttgcgaca ttgggcggca
     3961 ttctggcgtg ggaatttcgg gatcccatca ccaagaaaat cgttaaattt caaccgcagg
     4021 gcaatcttac tgccaataac ggcttcttgc tgcaacacta tgccaaacaa ggtttaggca
     4081 ttctttggag tccgcaagac agcattcaaa ccgagctgga taacggcagc ctcgtgccga
     4141 ttttaaccga ctgggcaatg cagtatgagg gctatcatct ctattacccc aaccgtcgcc
     4201 aaaattcgcc tttgctgaaa gcgttaattg atgtgctgag aggataggta aaaatgagcg
     4261 gtcgttttta gcaaaaaatt gaccaaaaaa cgaccacttg tagagaagct aaattatttc
     4321 acacgagaaa catattcgcc tgaacgggta tccacacgga taacttcacc gatttgaacg
     4381 aataaaggca cgtttaccac agcaccggtg cttaatgttg ccggtttacc gcctgtacct
     4441 gcagtatcgc cttttaagcc cgggtcggtt tcgacgattt ctaactcaac gaagtttggt
     4501 ggtgtgactg caattgctga accgttccat aaagtcacga tacattctgc attatcaact
     4561 aaccatttta cgttgtcgcc taacgctttt tcatctaccg agatttgttc aaaggtttca
     4621 gggtgcataa agtaccagaa atcaccatcg ctgtaagagt agttgtagtt agaatctact
     4681 acatctgctg cttcaaccga agtgcctgat ttgaagttga tctctaatac tttgccagag
     4741 attaatttac ggattttagt acgggtaaat gcttgacctt tacccggttt tacgaattcg
     4801 ttttcaacaa ttacacaagg ttcgccatct tgaataaatt ttaaaccttt tttaaagtcg
     4861 ttagtgctgt agctagccat tatttcctca attatttaaa aaataacaat aaattaacaa
     4921 attttgaacc caaccctatt gggtttgtca aaaagagcca tataataacc taaattcaca
     4981 aaattagcga aagatctctt gcgatttttg ctataatttt tcaaacttta ttgataagga
     5041 atgcgaatga aaaaactgat tactctttct gcgattttag ccgtttctgt tgcaaccgtg
     5101 gcagaggcaa aacgtggcgg tgggagcttc cgttcagtta gcccgtcaaa agcggtagca
     5161 actaagccgg tagcttctca aaaatcaacc acaaatcagc aaacgcaaca aaaagatgct
     5221 acttttgcca atacgccaaa cagtgcaact gcccagccac aagtgcgtca aggcagcacg
     5281 atggcaaatg tagcgatggg ggcagcagcc ggttatattt taggtgatat gatgtcatca
     5341 aatgcacatg caaatcagcc tagccagcct gcggatgcaa atgcacaagc ggtcaattct
     5401 gcacaaaatg ttgcaaatgg agcagttcct gctattgcag aatttaagag tattggcggt
     5461 caaatcgatc cgtttttagt agaaaaaacc gatggttatc gccgttattg tatttcaggc
     5521 gtgcaatacc tagtcgctgc acaaggtgta caaacctcac caactgtaat ggttaatcca
     5581 aatggcacgc cattacaatg taacttaatt ccataattat ttttatttgc gggcaggcaa
     5641 aaacctgccc cttgtatcta caatattcaa tgcaaccaat tcaaatcaaa ccgctttggc
     5701 taaccgagct ggctcaagcc tttaataacc ctatcgactt attacaattt cttgaactca
     5761 atccaaatga ttttgagggc gatatagctg cccgtaaatt atttgctctt cgagtgccaa
     5821 gaatgttcgc cgaaaaaatg gaaaagggta acccaaacga tccgcttttt ttgcaagcaa
     5881 tgtctttaca ggcggaattt attgaggcag aagggtttgt ggtagatcca ttggaagagc
     5941 aacaaagccc agcaccgaat attttacata aataccataa ccgtttgtta tttatgctaa
     6001 aaaacagttg tgcgattaac tgccgttact gttttcgccg tcatttccct tatgaggaag
     6061 tgaaaagcgg taaagctgtg tggcaacagg gactaactta tattgctgag catcccgagc
     6121 tggaggaagt gattctgtcc ggtggtgatc cgctgatggc aaaagatcag gacttagatt
     6181 ggatattaac gcaattagag cagatttcgc atatcaaaac cctgcgtatt cattcacgct
     6241 tgccggtcgt tattcccaat cgaattacga cagaattatg tgaacggtta tcaaaatctc
     6301 gcttgaatgt cgtgttggtt acccatatta atcacgccaa tgaaattgat gctgtttttg
     6361 caaacaaaat ggctcaactg aaaaaagccg gcgttgtatt gctgaatcaa tcggtgatgt
     6421 taaaaggtgt aaacgataat gctcaaacct taaaaaggtt aagtgataaa ctttttgaat
     6481 atgggatttt gccttactat ttgcatttat ttgataaagt ggcaggggcg agccatttct
     6541 atattgaaga tgaacaggct ggtgagattt accgagaatt acagcgtatc acttcaggct
     6601 atcttgtgcc aaagctggca agagaaatag cgaaagagcc aaataaaatt ttgaaaggtt
     6661 agcttaaaca gaaatcaaga aacaaaaggc gaaaatggta aaattttcgc cttttttaac
     6721 cgcttgttgc aagcggttaa attctcttac ttttttgcaa attagaagtt cgcattgcgt
     6781 ggagcacgtg ggaatgggat tacatcacga atgttttgta acccggttac atacacgatt
     6841 aaacgctcga aacctaaacc gaaacctgag tgtggcactg taccgtattt acgcaaatca
     6901 cgataccacc agtaatcttc agggtttaag cccatttcat tcatacgctg atctaacact
     6961 tctaaacgtt cttcacgttg agaaccaccg ataatttcac cgattcccgg agctaaaacg
     7021 tccattgcgg caaccgtttt gccgtcatca tttaaacgca tatagaaggc tttaatgtct
     7081 tttggatagt ttttcacgac caccggcgat ttaaagtgtt cttcggctaa gaaacgctcg
     7141 tgttcagagg aaagatcgat tccccaagac accgggaatt caaattcttt gccggatttc
     7201 aataagatct caatcgcatc ggtgtaatct acttgagcaa atggcgaatt gataaagttt
     7261 tctaaacgac taatcgcttc tttatcaacg tgtttaacga aaaattccat atcatcttta
     7321 cgttctgcaa gcaccgcttt aaacacatat ttcagcatat cttcggctaa ttttgcattg
     7381 tcgtttaagt cggcaaaggc aacttccggc tccatcatcc agaattctgc aagatggcga
     7441 gttgtgtttg agttttctgc acggaatgtc ggaccgaaag tataaacttt acttaaggca
     7501 cacgcatagg tttccgcatt taactggccg gatacggtta agaacgattc tttaccgaag
     7561 aaatcttggt taaaatccac tttgccgttt tcgccacgag gtagattttc taaatccagc
     7621 gtagaaacac ggaacatttc acctgcacct tcagtatctg aggcggtaat tacaggtgtt
     7681 gctacccaat agaaaccttg ctcgtggaag aaacggtgga tagcttgagc taaacagtga
     7741 cgaacacgag caactgcacc gattaagttc gtacgagggc gtaagtgagc gacttcacgc
     7801 agatattcga tagagtggcg tttcgctgcc attgggtagg tatcaggatc ttccacccaa
     7861 ccaagcactt ccactttact tgcttgtaat tcaacggctt gaccttcagc aggggattcc
     7921 acaactttac cggtcaccac cacagagcaa cctgcggtta aatgtaatac ttcggcatta
     7981 taatttggta gatcgttatt aataatcgct tgaattgggt caaagcaaga accatcgtaa
     8041 acgctcaaga aagataaacc ggcttttgaa tcacggcggg tacgcaccca accacgcact
     8101 gtcgcttctt cacctaccgc tatcttgccg gataggattt ccgaaactgt tggaaattta
     8161 gacattgaat acctc
//
LOCUS       DANLXF010000101         8105 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.101, whole genome shotgun sequence.
ACCESSION   DANLXF010000101 DANLXF010000000
VERSION     DANLXF010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8105
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.101"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            6..278
                     /locus_tag="PXD66_001475"
     CDS             6..278
                     /locus_tag="PXD66_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005823919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HDL6319768.1"
                     /translation="MPLLFEYDPNKSQINLAKYGIDFEQAKLLWEDERKVVLEACIEP
                     ELRYFLIGKIYDKYWTAFFTPRNDVIRLISVRRSRKKEISYYEKYH"
     gene            262..504
                     /locus_tag="PXD66_001476"
     CDS             262..504
                     /locus_tag="PXD66_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HDL6319769.1"
                     /translation="MRNTISAEELERIFDEGKEDILQYCDLSTAHRPGQMKRVNVDFP
                     NWMVEALDKQARHLGVPRQALIKMWIAEKLPVQSPR"
     gene            516..1184
                     /gene="rluA"
                     /locus_tag="PXD66_001477"
     CDS             516..1184
                     /gene="rluA"
                     /locus_tag="PXD66_001477"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HDL6319770.1"
                     /translation="MALIEYNPPLEPFLDEVYRDNHILVINKPSGLLSVPGNRPEYYD
                     SAMTRVQQKYGFTEPAHRLDMATSGILLFALSKAAEKELKRQFREREPKKHYIALLWG
                     KLGEKVGDKGEMNFPLICDWENRPRQKICYERGKKAITHYEVLAHYPNNIARVKLTPI
                     TGRSHQLRVHSLALGHPIIGDKFYANPLAKSLSPRLCLHAESLTITHPITRELMTFTA
                     EPTF"
     gene            complement(1162..1737)
                     /gene="ampD"
                     /locus_tag="PXD66_001478"
     CDS             complement(1162..1737)
                     /gene="ampD"
                     /locus_tag="PXD66_001478"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HDL6319771.1"
                     /translation="MPNLKIEQGCLTGQKQVPSPHFSQRPDQEDISLLVIHYISLPPE
                     EFGGNFIDLFFQGKLEPTAHPYFEEIKDLRVSAHCLINREGEITQYVNFNDIAWHAGV
                     SCFEGREKCNEYSIGIELEGSNNQPFTQAQYQSLANLTKAIQQSYPQITDERITGHCH
                     IAPERKIDPGQYFDWAYYKHLLNEAKKSAQQ"
     gene            1797..2639
                     /locus_tag="PXD66_001479"
     CDS             1797..2639
                     /locus_tag="PXD66_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HDL6319772.1"
                     /translation="MLSYRHSFHAGNHADVLKHIVQLLILRALKEKEKGFLYLDTHSG
                     VGRYSLLSFESEKTGEYLEGVARLWERDDLPEEVAFYIDELKKLNRGGKLKYYAGSPL
                     LAVQQLREQDRAILTELHPNDFPLLRQEFAKAKNVQTKRENGFQQLKSALPPKERRGF
                     VLIDPPYELKEDYELVVKAIEEGYKRFATGVYAIWYPVVLRQHTKRIVRGLMDTGIRK
                     ILQIELAVRPDSDQRGMTASGMIVINPPWQLETQMKKILPYLTEVLVPEGTGSWKVEW
                     ITPE"
     gene            2762..3403
                     /gene="pyrE"
                     /locus_tag="PXD66_001480"
     CDS             2762..3403
                     /gene="pyrE"
                     /locus_tag="PXD66_001480"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDL6319773.1"
                     /translation="MQQYKHDFIEFALSRNVLKFGEFTLKSGRISPYFFNAGLFNTGR
                     DLAKLGEYYAKAIQASGIEYDVLFGPAYKGIPIATTVAVALANLFDVDKPCCFNRKEA
                     KDHGEGGNLIGSPLTGKILLVDDVITAGTAIRESMEIINANNAQLAGVLIALNRKEKG
                     KGELSAIQEVERDYGCQVFSIVDFDDLVAFIETSPQYAPYLPKMREYREKYGV"
     gene            3577..4666
                     /locus_tag="PXD66_001481"
                     /pseudo
     CDS             3577..4666
                     /locus_tag="PXD66_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607068.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
     gene            complement(4731..5921)
                     /gene="metC"
                     /locus_tag="PXD66_001482"
     CDS             complement(4731..5921)
                     /gene="metC"
                     /locus_tag="PXD66_001482"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HDL6319774.1"
                     /translation="MSNYKNLSTKLVHTGRKKRNTQGAVNPVIQRASSLVFETLEEKK
                     HATQNRYKGALFYGRRGTLTHFSLQEAMCELEGGAGCYLYPCGAAAVTNAILAFVEQG
                     NHVLMTGAAYEPTQEFCNALLKRMGVSTTYYDPMIGEGLKALVQPNTKVLFLESPSSL
                     TMEVPDIPTLVRVAREINPEIVIMIDNTWSAGVLFPALEYGIDISIQAGTKYLVGHSD
                     VMIGTAVSNARCWDKLREGSYLMGQMVDADSAYITARGLRTLGIRLKQHEQSSIAVAN
                     WLAQRPEVKAVYHPALESCPGHEFFKRDFLGSSGLFSFELCEKLSNDKLANFLEHFEL
                     FSMAYSWGGFESLILANQPEEIAKIRPAIERKLTGTLIRIHVGLEAVEDLIADLEQGF
                     NRIR"
     gene            complement(5980..6519)
                     /gene="dsbB"
                     /locus_tag="PXD66_001483"
     CDS             complement(5980..6519)
                     /gene="dsbB"
                     /locus_tag="PXD66_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HDL6319775.1"
                     /translation="MLYFFKNLSQSRSAWFLLMLSCLILEGTALYFQHGMGLVPCVMC
                     IYERVALFGILFAGILGFLGNRYAIIRYLAILIWLGSAFKGLVLSIKHHDYQVNPSPW
                     NQCEFKVNFPQTLPLDQWLPQVFAPGPVNCSQSQWEMFGFSMAQWLIFTFAVYLIVAV
                     IVLISQFKRSRQRNNFMFR"
     gene            complement(6582..8105)
                     /gene="nhaB"
                     /locus_tag="PXD66_001484"
     CDS             complement(6582..8105)
                     /gene="nhaB"
                     /locus_tag="PXD66_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton antiporter NhaB"
                     /protein_id="HDL6319776.1"
                     /translation="MFKSFLGKSPEWYKLCILAFLIINPLIYFFINPFVAGWFLVAEF
                     IFTLSMALKCYPLQPGGLLAIEAVVIGMTNPHHIKEEIMANFDVILLLMFMVAGIYFM
                     KQLLLYIFTKLLVAIHSKKILSLSFCLSAAFLSAFLDALTVIAVIISVGTGFYGVYHK
                     VASGNNFEDSTDITNDEKIISNKEILEQFRGFLRSLLMHAGIGSALGGVMTMVGEPQN
                     LIIASQAEWGFIEFLLRALPVSLPVLICGIATCFLVEKYKLFGYGDRLPSKVWVVLAR
                     YSQLKEQKMTKQDRLKMLVQAIAGIWLIIGLALHLADVGIIGLTIIIICTAFCGITDE
                     HTLGKAFQESMPFTALLIVFFSVVAVIIDLKLFEPIIAFVLSAEPHSQLSLFYIFNGL
                     LSMISDNVFVGTVYINEAKTALDAGIIDREQFDLIAVAINTGTNLPSVATPNGQAAFL
                     FLLTSSFAPLIKLSYGRMVYMALPYTIVLSIVGFLGLEFLLPSMTEVMINWGWFITR"
BASE COUNT         2528 a         1607 c         1750 g         2220 t
ORIGIN      
        1 atgctgtgcc gttattgttt gaatacgacc cgaacaaaag ccaaattaat ttagccaaat
       61 atggtattga ttttgaacag gcaaagttgc tttgggaaga tgaacgaaaa gttgtactgg
      121 aggcttgcat tgaacccgaa ttacgctatt tcctgattgg caagatttat gataaatatt
      181 ggacagcgtt ttttacacca agaaatgatg ttattcgttt aatttcagtt agacgttccc
      241 gtaaaaaaga gatctcatac tatgagaaat accattagtg ccgaagaatt agaacgtatc
      301 ttcgatgaag gcaaagaaga tattttacaa tactgcgatc tttctaccgc tcatcgcccg
      361 gggcaaatga aaagggtgaa tgttgatttc ccaaattgga tggtagaagc gttagataag
      421 caagccagac atttaggtgt gcctcgccaa gccttgatta aaatgtggat tgcggaaaaa
      481 ttaccggttc aatcacctag gtaagcgagt ggcaaatggc attaattgaa tataaccctc
      541 cattagaacc atttttagat gaggtttatc gggataacca tattttagta attaataagc
      601 ctagtggttt attatcggtg ccgggcaatc gtcccgaata ttacgacagt gcaatgactc
      661 gagtacagca aaaatatggc tttaccgaac cggctcatcg tttggatatg gcaacaagcg
      721 gtattctttt gttcgcccta agcaaagcgg cggaaaaaga actaaaacgc caatttcggg
      781 aacgtgaacc gaaaaaacac tatattgcgt tactttgggg caaattaggt gaaaaagttg
      841 gggataaggg cgaaatgaat tttccgctga tttgcgattg ggaaaatcgc ccccgccaga
      901 aaatttgcta tgaacgaggc aaaaaagcca ttacccatta tgaagtgtta gctcattatc
      961 cgaataatat agcgagagta aaactcacgc ctatcacggg gcgttcgcat cagctacgag
     1021 tacatagcct tgcgttaggt cacccgatta ttggcgataa gttttatgct aatccgctgg
     1081 caaaaagttt atcgccccgt ttatgtttgc acgcggagtc actaacaatt actcacccga
     1141 ttacaaggga gttgatgacg tttactgctg agccgacttt ttagcctcgt tcagtaggtg
     1201 tttgtaatat gcccaatcaa aatattgccc cgggtcgatt ttccgttccg gggcaatatg
     1261 gcaatgcccg gtgatgcgtt catcggtaat ttgtggatag ctttgctgaa tggctttggt
     1321 caaatttgcc aaagattggt attgagcttg agtaaacggc tgattattac tgccttccag
     1381 ttcaatgcca atcgaatatt cattgcattt ctcccgccct tcaaaacaag agacgccggc
     1441 gtgccacgct atatcgttaa aattcacata ttgcgtgatt tccccctccc tattaatcaa
     1501 acaatgggcg gaaacccgta gatctttgat ttcttcaaaa taagggtgag cggttggttc
     1561 gagtttacct tggaaaaaca gatcgataaa atttccacca aattcttccg gcggtaagct
     1621 gatgtagtga ataaccaaca aggaaatatc ctcttgatca ggacgttggc taaaatgcgg
     1681 agacggcact tgtttttgac cggttaaaca gccctgttcg atttttaaat ttggcatagt
     1741 ctaaataggg tgaatacgtt aaaatatggc tcattttact agaaaaagag aagaatatgt
     1801 taagttatcg ccacagtttc cacgcaggca accacgctga tgtgctaaaa cacattgtcc
     1861 agcttttgat cttgcgagca ttaaaagaaa aagaaaaagg ttttttatat cttgatactc
     1921 actccggtgt ggggcgttat agtctgttaa gttttgaatc ggaaaaaaca ggcgaatatt
     1981 tagagggcgt tgcccgttta tgggagcgtg atgatttacc ggaagaagtg gcattctata
     2041 ttgatgagct gaaaaaatta aaccgtggcg gcaagttaaa gtattacgca ggctccccat
     2101 tattggcggt acagcaactg cgtgagcaag accgagccat tcttaccgaa cttcacccca
     2161 atgattttcc gctgttacga caagaatttg ccaaagcgaa aaatgtgcaa accaagcgag
     2221 aaaatggctt tcaacaatta aaatccgccc tgccgcctaa agagcgtcga ggttttgtgt
     2281 tgattgatcc accttatgaa ttaaaagaag attatgaatt ggtggttaaa gcgatagaag
     2341 agggctacaa acggtttgcc actggtgtgt atgccatttg gtatccggtg gtgcttcgcc
     2401 aacataccaa acgtatagtg cgaggtttaa tggataccgg cattcgcaaa attctacaaa
     2461 ttgagcttgc cgttcgtcct gatagcgacc aacgtggaat gacggcaagt ggtatgattg
     2521 tgatcaaccc accgtggcag cttgaaaccc agatgaaaaa aatcctgcca tatttaaccg
     2581 aggtgcttgt gccggaagga acaggttctt ggaaggtgga gtggattacg cctgagtaaa
     2641 ttaaatacgg atagcacaag cgtggacgct tgcgccatca taaattctga tttgcaatat
     2701 tttttagaaa aataaccgct tgtcttatca agaagtgggg gaaatataaa aggaaaaaac
     2761 gatgcaacaa tacaaacacg actttattga atttgcctta agccgcaatg tgctgaaatt
     2821 tggcgaattt accttaaaat caggcagaat cagcccctat tttttcaatg ctgggctttt
     2881 taacaccggg cgcgatttag ccaaattagg cgaatattac gccaaagcca ttcaagccag
     2941 cggcattgag tatgatgtgt tattcgggcc ggcttataaa ggcattccga ttgcaaccac
     3001 ggttgccgtg gcactagcca acctgttcga tgtggataaa ccttgttgct ttaaccgcaa
     3061 agaggcaaaa gatcacggtg aaggcggtaa tttaatcggt tctcccttaa cgggcaagat
     3121 tttgctggtg gacgatgtga ttaccgcagg cacggcaatt cgtgagtcga tggaaattat
     3181 caatgccaat aatgcccagc ttgccggtgt gctgattgcg ttaaaccgta aagagaaagg
     3241 caagggcgag ttgtctgcga ttcaagaagt cgaacgtgat tacggttgcc aagttttctc
     3301 gattgtggat tttgatgatt tggtagcatt tattgaaacg tcgccacaat acgctcctta
     3361 tttgccgaaa atgcgtgaat atcgtgaaaa atacggtgtt taacttttgc aaaaaaacag
     3421 caaaaactca ccgcttgtca ctctctaatc tcaccattat gaatcacttt catcttttgg
     3481 tgagattttt tcaattaaaa agccgataaa ttgattgtga gctttagcta attggcttag
     3541 gataaactcc tgtaatacag gagttggaga actaaaatgg cacatcaaaa attacctttt
     3601 cacgcagaca ccgttggctc atatttgcgt tccgatgctt ggaaaaaagc tcacgcagac
     3661 tataaagcag gcaacatttc cctcgaacaa cgtgatgaaa ttgtagaagc ggaagtgaaa
     3721 aaattagtac aagcatcaat tagatgcggg cattcaagtt gttacagacg gcgaatatca
     3781 ccgctcttgg tggcatattg atttcttgga aaatctgaac ggcattgaag gctatgtgcc
     3841 ggaaaaagcc tatgcgttta aaggggttga ggttcgccct tataatacac gttgttgcgg
     3901 taaagtgtct tggaatccga atcacccttt tattgagcat ttcaaaaaat tcaatgcgat
     3961 tgtaggtggt cgtgcggtgg cgaaatttac cattccaagt ccaaaccaat taatgtaccc
     4021 gattcagtgg gatcacggca tttacccaaa ccgtgaggaa tttgccaaag atgtgcaaca
     4081 ggcttataaa gaggcgatta aagcgttcta tgatgccggc tgtcgttatt tgcaaattga
     4141 tgatgtctat tgggggtcgt tgtgtaataa cgttggtcaa ccgacatttg aagccgataa
     4201 agctcaagcg gtggcgaata ttcaagccat tcttgccgat aagccggaag atttaaccat
     4261 taccacccac gtttgccgag gcaactataa atcttcttat ttattgacag gggcttatga
     4321 tccgattgcc gacggcttat ttgctcaaac caactatgat gggtatttct tagaatatga
     4381 tgacgagcgt tcaggcggtt ttgagccgtt aaaacatttt gccaacaaca aagggcgtgt
     4441 ggtattgggc ttaattagtt caaaattccc tgaattggaa gataaggaag cggttaaagc
     4501 ccgtattcaa gaagcggcaa aatatgtgcc gttagagcag ctttgcctaa gcccgcaatg
     4561 tggttttgcc tcaaccgaag agggcaatat tatgtcggaa gcacagcaat gggcgaaagt
     4621 tcgtcttgtg gaggaaatcg cccaagaagt ttggggcgaa gattaattag agtgaaaaat
     4681 gcccgtaaca agcgggcatt ttttgtgaat tttttgcaaa ttcaagccat ttagcgaatg
     4741 cggttaaaac cttgttctaa atcggcaatc aaatcttcca ccgcttccaa gccaacgtga
     4801 atgcgaatta atgttcccgt cagtttgcgt tcaatcgccg ggcggatttt ggcaatttct
     4861 tccggctgat tggcaagaat cagcgattca aacccacccc aagaatacgc cattgaaaac
     4921 agttcaaaat gctctaggaa gttggctaat ttgtcattcg agagcttttc gcaaagttca
     4981 aatgaaaata gaccgcttga acctaaaaaa tcccgcttaa aaaattcgtg ccccgggcag
     5041 ctttccaacg ccgggtgata aactgctttc acttcagggc gttgagccag ccagttagct
     5101 accgcaatgc tactttgttc gtgctgtttt aagcgaatgc ctaaggtgcg tagcccccga
     5161 gcggtgatgt aagcggaatc agcatccacc atttgcccca ttaaataaga gccttcacgc
     5221 agtttatccc aacaacgggc gttagaaacc gcagtaccga tcatcacatc agaatgcccg
     5281 actaaatatt tagtgccggc ttgaatggaa atatcaatgc catattctaa agcagggaac
     5341 agcacaccgg cagaccaagt gttgtcgatc ataatcacga tttctgggtt gatttcacga
     5401 gcaacacgca cgagagtcgg aatatcaggc acttccattg ttagcgagct cggcgattct
     5461 aaaaaaagca ctttggtgtt cggttgcaca agggctttca agccttcacc aatcatcggg
     5521 tcataataag tagttgaaac acccatacgt tttaacagcg cattacagaa ttcttgtgtc
     5581 ggttcgtaag ctgcgcctgt cattaaaacg tggttgcctt gttccacaaa agctaaaatc
     5641 gcattggtga ctgccgccgc accgcaaggg taaaggtagc aacctgctcc accttctaat
     5701 tcacacatcg cttcttgtaa tgaaaaatga gtgagagtgc cacgtctgcc ataaaataat
     5761 gctcctttgt agcggttttg tgtagcgtgt tttttctctt ctaaggtttc aaacactaaa
     5821 gatgaagcac gctgaatcac cgggttaact gctccttgtg tgttacgttt tttacgccct
     5881 gtatgaacaa gtttggtgga aagattttta tagttagaca taacttttcc taaagtaaaa
     5941 tgcatagggg caaatcaaca tttgcccctt tattaataat taacgaaaca taaagttatt
     6001 tctttgacgg ctacgtttaa attggctgat tagcacaatg accgcaacaa ttaagtaaac
     6061 cgcaaaagta aagattagcc attgtgccat tgaaaagccg aacatttccc attggctttg
     6121 gctacaatta accggaccgg gggcaaacac ttgtggtagc cattggtcaa gcggcaaggt
     6181 ttgcgggaaa ttaactttaa attcgcattg attccatggt gatggattga cctgataatc
     6241 gtggtgtttg attgaaagta ctagcccttt aaatgcgcta ccaagccaaa ttagaattgc
     6301 caagtagcga ataatcgcat atctattacc aagaaatcct aaaataccgg caaataaaat
     6361 accaaataaa gcaactcgct cataaataca cattacacaa ggtactaatc ccatcccatg
     6421 ttggaaataa agcgctgtgc cttcaagtat aagacagctt aacattagta aaaaccatgc
     6481 actgcgagat tgtgatagat ttttaaaaaa atagagcata gttattcctc attttgtgaa
     6541 cttaaggcat tctaccttat ttggtatgag ttatctataa actaacgtgt aataaaccat
     6601 ccccaattta tcattacttc agtcatagaa ggtagtaaga attctaaccc taagaaaccg
     6661 acaatagata gaacaatggt ataaggtaat gccatataga ccattctgcc gtaagacagt
     6721 ttaattaacg gcgcaaagga ggacgttaat aagaataaga aagcagcttg tccgtttggg
     6781 gtagcaacag aaggaagatt tgtgccagta ttaattgcaa ctgcaatcag gtcgaactgc
     6841 tcacgatcta taatgccggc atctagtgcg gttttagctt cattaatgta aacagtaccg
     6901 acgaagacgt tatctgaaat catcgagagc aaaccattga aaatatagaa taatgaaagc
     6961 tgtgaatgtg gttcggcaga taacacaaag gcaatgatcg gctcaaataa ttttaaatca
     7021 ataatcacgg caacaaccga gaaaaacaca ataagcaatg cagtaaaagg catagattct
     7081 tggaatgctt tccctaaagt gtgttcatca gtaatgccac aaaatgcagt gcaaataatg
     7141 atgatggtta agccaataat ccctacatcg gcaagatgta gcgctaagcc aataattaac
     7201 caaattccag caatggcttg caccagcatc tttaagcgat cttgtttcgt cattttttgt
     7261 tcttttagtt ggctatagcg agcgagtacg acccaaactt tgctaggaag ccggtctcca
     7321 taaccaaata atttatattt ttctacaaga aagcaggtcg caatcccaca aattaacacg
     7381 ggtaagctaa ctggtaatgc tctaagtaag aattcgataa atccccattc cgcttgggag
     7441 gcaataatta agttttgtgg ttctcccacc atggtcatta cgccacctaa agctgagcca
     7501 ataccggcgt gcatcaataa acttcttaaa aaaccacgaa attgctctaa aatttcttta
     7561 ttagagatga ttttttcatc attggtaata tcagtagaat cttcaaagtt attacctgat
     7621 gcgactttat gatatacgcc gtagaaacct gttccaacgc taatgattac agcaataacc
     7681 gttaaagcat caagaaaggc ggataaaaaa gcagcgctta agcagaaaga aagggaaaga
     7741 atttttttcg aatgaatggc gacaagcagt ttggtgaaaa tataaagcaa taattgcttc
     7801 ataaagtaaa taccggcaac cataaacatt aaaagaagaa taacatcaaa gtttgccata
     7861 atttcttctt taatgtggtg aggatttgtc ataccgatta caactgcttc aatcgctaat
     7921 aaaccacctg gctgtaatgg ataacatttt aatgccatag agagagtaaa aataaattca
     7981 gcgactagaa accagcctgc aacaaaagga ttgataaaga aataaatgag aggattgata
     8041 attaaaaatg ccaaaatgca caatttatac cactcggggc tttttcctaa gaaacttttg
     8101 aacag
//
LOCUS       DANLXF010000102         8103 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.102, whole genome shotgun sequence.
ACCESSION   DANLXF010000102 DANLXF010000000
VERSION     DANLXF010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8103
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.102"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            2..1108
                     /gene="rnd"
                     /locus_tag="PXD66_001485"
     CDS             2..1108
                     /gene="rnd"
                     /locus_tag="PXD66_001485"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005556401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HDL6319777.1"
                     /translation="MHYQWVETNEQLEAVCEQARKTKVVALDTEFIRTRTYYPILGLI
                     QLFDGQQVSLIDPNTISDFSPFVSLLADKNIIKVLHACSEDLEVFEYQFKQLPEPMLD
                     TQVMAAFAGIGISMGFAKLVSHYLNIELDKGASRTDWLARPLSAEQLQYAAADVWYLL
                     PVYEKLAQALTKSTWQSAVAEECVALLKKAKQPINKEKAYKNIANAWRLNREELAILQ
                     VLAKWRIEEAQKRNLALNFVVKEASLVQIAKSCPKHTSSLLEFMHPNEVRIHGKKILW
                     LVEQGKAIESNDYPELIQRLVDEKGYKYYMQAMLQKLLEIQPLDIVPELVASKRQLNQ
                     LFKWYIDGKPSEKIPELLIGWRKPFGEQLLSVLS"
     gene            1165..1647
                     /gene="lrp"
                     /locus_tag="PXD66_001486"
     CDS             1165..1647
                     /gene="lrp"
                     /locus_tag="PXD66_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005695827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HDL6319778.1"
                     /translation="MENKKPPKALDAIDLKILNELQRNGKISNIELSKRVGLSPTPCL
                     ERVKRLEKNNVIMGYKALLNPELLDAPLLVIVEITLVRGKPDVFEEFNKAIQQLDEIQ
                     ECHLVSGDFDYLLKTRVADMVSYRKLLGTTLLRLPGVNDTRTYVVMEEVKQTNYLLLK
                     "
     gene            1658..4318
                     /locus_tag="PXD66_001487"
     CDS             1658..4318
                     /locus_tag="PXD66_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HDL6319779.1"
                     /translation="MIEQLKPHLKGKENLIKLMLLLTGFLGVYLLIAWASYSPLDNAW
                     SVASSITQTTLNKTGHFGAWSIDMLYAMFGKVAVLVPIALIIFSIYSLGMGLSVDMKW
                     KTVFIRLASFLLLMVGLAGLFSVLFSNSAYYLSGGFVGGVWETILSDTIGQFGALLIA
                     MLFTIAGLYFCSVQSLLPILTQFYEWIMAKSEESKTAESLQHLQNTEQKQPLVTENEA
                     VEVVDDHKPQFTDISAFKRPNISGLRHSENVEIDNREMYEIERDLELPTINILIQPDM
                     VNQVAEQATSGYELPNEESNVLQMPTIRLNTAIENEVDTIENSSSLEDLKIAESMLPQ
                     IQLDNDIEDTEEAIDDVREITPEFMVTPPKAMKDVSEQPAYPKGYGDTLIHPLLQRKV
                     ITEKPTTPLPTLDLLDKAPIQSQQITEQEIRETSVRLEAELANFGVKATVEDVLVGPV
                     VTRYEIQPAAGVKASKITNLASDIARGLMFKAIRITEVIPNKPYMGIETPNKHRETVW
                     LRDVLDSDEFRNTTATLPMALGKDISGEPVVVDMAKMPHLLVAGQTGGGKSVGVNTMI
                     LSLLFKLTPEQVRFIMIDPKVVELSIYNDIPHLLTPVVTDMKKAANALRWAVEEMERR
                     YLLVSHLQVRNIEGYNAKIDQAAAMNLPIPDPTWRPGDSMDSLPPPLQKLSYIVLIVD
                     EFADLMMSAGKEVEEYIMRIAQKARAVGIHLILATQRPSTDVITGVIKANIPSRIAFT
                     VASQIDSRTILDSGGAEALLGRGDMLYSGAGSPDIIRIHGAFMKDEEVQRVADNWRAR
                     GKPNYLESIVESRSEEADGKNDGGTGDLDPLFDEVVEYITETGSVSISNIQRRFSLGF
                     NRAARIVDQMEAQGIVSEPLKGGKREVLAR"
     gene            4470..5309
                     /locus_tag="PXD66_001488"
     CDS             4470..5309
                     /locus_tag="PXD66_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HDL6319780.1"
                     /translation="MRLDDIKESKNVEDRRFNNVGRTKVGRGKGGILTFIIVLVGAYY
                     GVDLTGLMGGGVEYEEQTSALNESEEKQLRTLSAKVLTTTENIWTSYFQQNGLTYKEP
                     SLVLYRGAIQTACGTGQSAMGPFYCPVDQKVYLDLSFYDDMRKKLRASGDFAFSYVIA
                     HEVGHHVQNLLGITSKTQRAQMKASSKAEANQISVNVELQADCFAGVWGHQLAKANRL
                     EEGDVEEAFNAAQAVGDDRLQQQSRGYVVPDSFTHGSSAQRLAWFRKGLTTGNPAVCN
                     TFN"
     gene            5398..6480
                     /gene="aroG"
                     /locus_tag="PXD66_001489"
     CDS             5398..6480
                     /gene="aroG"
                     /locus_tag="PXD66_001489"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HDL6319781.1"
                     /translation="MSYIKNDDVRITSIEELLPPIALLEAYPASDVAAETVAKTRQSI
                     HKILHGADDRLLVVIGPCSIHDPKAALEYAQKIKAMRADPKINQNLEVVMRVYFEKPR
                     TTVGWKGLINDPYLNETFALNDGLRIARKVLSDINDLSVPTAGEFLDMITPQYVADFM
                     SWGAIGARTTESQVHRELASGLSCAVGFKNGTNGSVKIALDAISSAKSPHHFLSVTKF
                     GHSAIVSTAGNPDCHIILRGGDSATNYDADSVAAACAAIEKSGGRPHVMIDFSHANSQ
                     KQFKRQMDVCADVCNQIAGGSDFISGVMIESHLVEGRQDLIEGKGLEALVYGQSITDG
                     CIGWEDSEKALFALAEAVEKRRNFAK"
     gene            6567..7259
                     /gene="tsaB"
                     /locus_tag="PXD66_001490"
     CDS             6567..7259
                     /gene="tsaB"
                     /locus_tag="PXD66_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDL6319782.1"
                     /translation="MKPSILALDTATEACSVALQYNGNITFLDEISPRSHTQRILPMV
                     DELLNQANIELKEVNYLVFGRGPGSFTGVRVGVSVAQGLAMGANLPVVAVSNLKAMAE
                     EAYQKLGATKVIALIDARMNEVYFAQFERKGEEWNEVVAEQVCSPEVAISQFQMSEET
                     VVVGTGWAAYSQFSEQNLPLVISDITLPSSRYMLSIAETEIQKGNIQSAFEIEPVYLR
                     NEVTWEKLPHKQ"
     gene            complement(7276..8085)
                     /locus_tag="PXD66_001491"
     CDS             complement(7276..8085)
                     /locus_tag="PXD66_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDL6319783.1"
                     /translation="MTMTVIIILIICGIIVNITSALFGIGGGVLMMPILHSLFPELPL
                     QVISACSLTIVIGSATINLCLFHHQQIKINRKNVLIWSLAMMLGVQIGFELSFLFSDN
                     SIIAIFILTLLTLSFKTLFHKPRVKAIVPPKPKEAVSNATMCSLGGLIAGITGIGGGS
                     IMAPIVTQIRSVSPKQVAVYTNYMMVIGGIGNLYGYLSRPFVLNSTLATWQIGYVNFA
                     IVGTVVISSLFTVYFSMKLRNILSPELTTKLLGVILFTIACYMGFLQYLFT"
BASE COUNT         2555 a         1479 c         1814 g         2255 t
ORIGIN      
        1 gattcactac caatgggttg aaacaaatga gcaattagag gccgtatgtg aacaggccag
       61 aaaaacaaaa gtagttgcct tagatacaga gtttatccgt acacgtacct attatccgat
      121 attgggttta attcaactct ttgatggcca acaggttagc cttattgatc caaatacgat
      181 ttctgacttc tcgccttttg tgtcattatt agccgataaa aatatcataa aagtattaca
      241 tgcctgtagt gaggatttag aagtttttga gtatcaattt aagcaattgc cagagccgat
      301 gttggatact caggtgatgg cagcctttgc cggcattggt atttcgatgg ggtttgcaaa
      361 attggtttcc cactacttaa atattgaact agacaaaggc gcttcccgaa ccgattggct
      421 tgccagaccg ctaagcgctg aacaattaca atatgcggca gctgatgttt ggtatttatt
      481 gccagtttat gagaaattgg cacaagcatt aacaaaaagc acttggcaaa gtgctgtagc
      541 agaggagtgt gtcgctttgt tgaagaaagc gaaacagcct atcaataagg aaaaagccta
      601 taaaaatatt gccaatgctt ggcgattgaa tcgggaagag ttagccatat tacaagtatt
      661 agcgaaatgg cggattgaag aagcacaaaa gcgcaatctt gccttaaatt ttgtcgtgaa
      721 ggaggcaagt ttagttcaaa tcgcaaaaag ctgtcctaaa cacacttctt cgctgttaga
      781 atttatgcac ccaaatgaag tcagaattca cggaaaaaaa atactatggt tggttgagca
      841 aggaaaagca atagaatcga atgattatcc tgaactcatt cagcgcttgg tggatgaaaa
      901 gggttataaa tattatatgc aagcgatgtt gcaaaaatta ttagaaattc aaccgcttga
      961 tatcgtaccg gagttggttg caagtaaacg tcaattaaat cagctattta aatggtatat
     1021 tgatggaaaa ccatcagaaa aaattcctga attattaatc ggttggcgaa aaccttttgg
     1081 tgaacagctg ctatcggttt tatcttaaat ttcattcggg ttacatagac tatgtacagt
     1141 ataattgcct aaaaaggagt ttatatggaa aataaaaaac caccaaaagc attagatgcg
     1201 attgatttaa aaatcttaaa cgagctacaa cgtaacggga aaatttctaa tattgaatta
     1261 tcaaagcgag ttgggctttc tccaacgccc tgcttagaac gggtaaaacg gctagaaaaa
     1321 aataacgtaa ttatgggcta taaagcttta cttaaccctg aattacttga tgccccttta
     1381 ctggtgattg ttgaaattac tttggtgcgt ggtaagcctg atgtatttga agaatttaat
     1441 aaagcgattc aacaacttga tgaaattcaa gaatgccacc tggtatcggg ggattttgat
     1501 tatctgttaa aaacccgtgt ggcggatatg gtatcgtacc gtaagttatt aggcacaaca
     1561 ttgcttcgtt tgcccggcgt aaatgacact cgaacctatg tggtgatgga agaagtaaaa
     1621 caaaccaact atttgttgtt aaagtaagga gaattgggtg attgaacaac ttaaaccaca
     1681 cctaaaaggc aaagagaact tgatcaaact tatgcttttg ttgaccggct ttttgggcgt
     1741 ttatttgcta attgcttggg caagttatag tccattagat aatgcttggt ctgttgcaag
     1801 cagcattaca caaacaacct tgaataaaac aggtcacttt ggtgcttgga gtattgatat
     1861 gctctatgca atgtttggta aggttgcagt tttagtacct attgcattaa ttattttctc
     1921 aatttatagc ttgggaatgg gtttaagtgt cgatatgaaa tggaaaactg tcttcattcg
     1981 cctagccagt ttcttattat tgatggtcgg tcttgccggg ttatttagcg tgcttttttc
     2041 aaactcagct tattatctgt cgggtggctt tgtcggtgga gtttgggaga caatattatc
     2101 tgatactatc ggacaatttg gtgccttatt gattgcaatg ctttttacta tagctggctt
     2161 atatttttgc tctgttcaat cattattgcc aatattaacg caattttatg agtggataat
     2221 ggcaaaatca gaagagagca aaacagctga atctcttcag catttgcaaa atactgagca
     2281 aaaacaaccg cttgtaactg aaaatgaagc ggtggaagtt gtagatgacc ataaaccgca
     2341 atttaccgat atctccgcct ttaaacgccc aaacattagt ggtttaagac actcggaaaa
     2401 tgtagagata gataaccgag aaatgtatga aattgaaaga gatcttgagc taccgactat
     2461 taatatttta atacagcctg atatggttaa ccaagttgcg gaacaagcta catcgggcta
     2521 tgagttacca aacgaagaat ccaatgtatt gcaaatgccg accattcgct taaataccgc
     2581 tatcgaaaac gaggtggata caatcgaaaa cagttcatca ttggaagacc tgaaaatagc
     2641 agaatctatg ttacctcaaa ttcagcttga taatgatatt gaagatactg aagaagctat
     2701 tgacgatgtg cgtgaaatta caccggaatt tatggtaacg ccgcccaagg cgatgaaaga
     2761 tgtgtctgag cagccagcct atccaaaagg ctatggtgat acgttaattc acccgttgtt
     2821 acaacgaaaa gtgataaccg aaaaaccgac aacaccattg ccaacgctag atttgttgga
     2881 taaagcaccg attcaaagcc agcaaattac cgaacaggaa atacgagaaa cctctgtgcg
     2941 gctagaagca gaattagcaa actttggcgt aaaagctacc gttgaagatg tattggtcgg
     3001 tccggtagtg acccgttatg aaatccaacc ggctgccggg gtaaaagcct ccaaaattac
     3061 caatttagcg agcgatatag ctcgtggctt aatgtttaaa gcgattagga ttactgaagt
     3121 tatcccaaac aaaccttata tgggtattga aactccgaat aaacaccgtg aaacagtgtg
     3181 gttgcgtgat gtgctagaca gcgatgaatt ccgcaatacc actgcaacat tgccaatggc
     3241 actaggtaaa gacatcagtg gtgagcctgt ggtggtcgat atggcgaaaa tgccacactt
     3301 actggtggca gggcagaccg gtgggggtaa atcggttggg gtgaatacga tgattttaag
     3361 tctattgttc aaacttacac cagaacaagt ccgctttatt atgattgacc ctaaagtggt
     3421 ggagctttcc atttataacg atattcctca cttattaacg ccggtagtaa ccgatatgaa
     3481 aaaagccgcc aacgcactac gctgggcggt ggaggaaatg gaacgccgtt atttgctggt
     3541 aagccattta caggtgcgta atattgaagg ctataatgcg aaaatcgacc aagcggcagc
     3601 gatgaatttg ccgattccag atccaacgtg gcgaccgggc gattcaatgg atagccttcc
     3661 gcctccgcta caaaaattaa gctatattgt gctgattgtc gatgagtttg cggatttaat
     3721 gatgtctgcc ggtaaagaag tggaagagta cattatgcga attgctcaaa aagcccgagc
     3781 ggtgggaatt cacttaattc ttgctaccca acgtccgtca accgatgtga ttaccggcgt
     3841 gattaaagcc aatattccaa gccggattgc gtttacagta gcgagccaaa ttgactcaag
     3901 aactatttta gattcaggtg gagcagaggc attattaggg cgaggcgata tgctctattc
     3961 aggagctggc agcccggata ttattcggat acacggtgcg tttatgaaag acgaagaagt
     4021 gcaacgcgtg gcggataatt ggcgggcaag aggaaaacca aactacttgg aaagcattgt
     4081 ggaatcccgc tcggaagagg ctgatggcaa aaacgatggc ggaacgggcg atttagatcc
     4141 gctatttgac gaggttgttg aatatatcac ggaaaccggt tcggtctcta tcagtaatat
     4201 tcaacgccgc ttctcgcttg gttttaaccg tgcggctaga attgtggatc aaatggaagc
     4261 tcaaggcatt gtttctgagc cacttaaagg tggcaaacga gaagtgctgg cgaggtaaaa
     4321 cacatacaag cggttagatt tctgtgaaat tttgcaaatt ttgacaaaaa tctaaccgat
     4381 tgtatttatc taatgctaaa taagcatagt ataaggctag tctttgggct aaaattaacg
     4441 caatttttac atcttttaag gttgaatata tgcgattaga tgatattaaa gaaagtaaaa
     4501 atgttgaaga cagaagattc aacaatgtgg gtagaactaa ggttggtaga gggaaaggtg
     4561 gaatattaac ctttattatc gtgcttgttg gagcttatta tggtgttgac ttaacaggtt
     4621 taatgggcgg tggcgtagaa tatgaggagc agacatcagc attaaatgaa agtgaagaaa
     4681 aacagctaag aaccttatcc gctaaagtat taacgaccac agaaaatatt tggacttcct
     4741 acttccagca aaatggtcta acttataaag agccgtcttt ggtgctttac cgtggggcaa
     4801 ttcaaactgc ctgtggcacc ggtcaatctg caatgggtcc tttctattgc cctgtcgatc
     4861 aaaaggttta tttagattta tcattctatg atgatatgcg taaaaaatta agagcatcgg
     4921 gcgattttgc cttctcttac gtgattgcac acgaggttgg ccaccacgtc caaaatttat
     4981 tgggcataac aagcaaaacc caacgggctc aaatgaaagc ctcatctaaa gccgaagcaa
     5041 accaaatttc agtcaatgta gaattgcaag ctgactgttt tgccggcgtt tggggacacc
     5101 aactcgcaaa agccaaccga ttagaggaag gcgatgtgga agaagccttt aatgctgccc
     5161 aagcagttgg agatgaccga ttacagcaac aaagtcgtgg ctatgtcgtg ccggatagct
     5221 tcacccacgg ctcatctgca cagcgtttgg cttggtttag aaaaggttta accacaggaa
     5281 atcctgcagt ttgtaataca tttaattaaa aaataattaa attatttgaa atctttttat
     5341 tatcgtacta ctatgatagt acgattttat tttcatttta tttagagagt gaacacaatg
     5401 tcttatatca aaaatgacga tgtcagaatt accagtattg aagaattact accacctatt
     5461 gcattactgg aagcctatcc ggcaagtgat gttgcagcgg aaaccgttgc taaaacccgt
     5521 caatcaatcc acaaaatttt acacggtgcg gatgatcgct tattagtggt gattggacct
     5581 tgctccattc acgacccgaa agcggcatta gaatatgcac aaaaaattaa agcaatgcgt
     5641 gctgatccga aaattaacca aaacttagaa gtggtgatgc gtgtctattt tgaaaaaccc
     5701 cgtacaaccg ttggttggaa aggcttaatt aacgaccctt atttgaacga aacctttgcc
     5761 ttaaacgatg gtttacgcat tgcccgtaaa gtattatctg atattaatga tttaagcgtg
     5821 ccgactgccg gcgagttttt ggatatgatt acaccacaat atgtggctga ctttatgagc
     5881 tggggagcaa ttggggcgag aacgactgaa tctcaagtcc accgtgaatt agcctctggt
     5941 ttatcctgtg cggtaggttt taaaaacggc acgaatggta gtgtgaaaat tgcattagat
     6001 gcaatttctt ccgcaaaatc gccacaccat ttcttatcgg tcactaaatt cgggcattct
     6061 gcgattgttt caacagcagg aaatcctgac tgtcacatta ttttgcgtgg tggcgatagc
     6121 gcaaccaact acgatgctga ctccgttgca gcggcttgtg ctgcgattga gaaatccggc
     6181 ggacgtccac acgtgatgat cgatttcagc cacgctaaca gccagaagca gttcaaacgc
     6241 caaatggacg tttgtgctga tgtgtgcaac caaattgcag gtggatcaga ctttatttca
     6301 ggtgtgatga ttgaaagcca cttggtagaa ggtcgccaag acttaattga aggcaaaggt
     6361 ttagaggcgt tagtttacgg acaaagtatt acagacggct gcattggctg ggaagatagt
     6421 gaaaaagcgt tattcgcact tgctgaagcg gttgaaaagc gcagaaattt tgcaaaataa
     6481 tcttttcttg aaccgagggc gaatataatt cgccctttat ttgtttaacc ttaaagcaaa
     6541 tttaatttat tcaaactaaa catatgatga aacccagcat tcttgcttta gatactgcaa
     6601 cagaggcttg ctctgtagcg ttacagtata acggaaatat cacgttctta gatgaaatca
     6661 gccctcgttc ccatactcaa cgtattttgc cgatggtaga tgaattactt aatcaagcca
     6721 atatcgaact caaagaagta aattatttag tattcggacg tgggccgggc agttttaccg
     6781 gtgtgagggt cggtgtaagt gtggcacaag ggttggcaat gggagcgaat ttgccggtgg
     6841 tagctgtttc taacttaaaa gcaatggcag aagaagcata ccaaaaatta ggtgcaacaa
     6901 aggtaattgc cttaattgat gcccgaatga atgaagttta ctttgctcaa tttgaacgaa
     6961 agggtgaaga atggaatgaa gtggtggctg aacaagtctg ttcgccagaa gtcgcaataa
     7021 gtcaattcca aatgagtgaa gagactgtgg tggttggcac aggttgggcg gcatattcgc
     7081 aattttctga gcaaaatcta ccgcttgtta tcagtgatat taccttgcct tccagccgat
     7141 atatgctttc gattgctgaa actgagattc aaaaaggcaa tatccaatct gcttttgaaa
     7201 tagaaccggt gtatttgcgt aatgaagtta cttgggaaaa actacctcat aagcaataat
     7261 ctattgaact tatggttaag tgaataaata ttgcaaaaag cccatataac aggcaatagt
     7321 gaataggatt acaccaagta atttagtagt taattcggga ctcaagatat tgcgtaattt
     7381 cattgaaaaa tagacagtaa agagagaact tatcactacg gtgccgacaa ttgcaaaatt
     7441 cacatagccg atttgccaag ttgctaaggt tgaattcaac acaaaagggc gggataagta
     7501 gccatataaa ttgccgattc cgccaatcac catcatatag ttagtataaa ctgcaacctg
     7561 tttcggtgaa accgagcgta tttgcgtgac aatcggagcc ataatcgaac cgccgccaat
     7621 gccggtaata cctgcaatta aaccgcctaa actgcacatt gtggcattag atactgcttc
     7681 ttttggttta ggtggtacta ttgctttaac tcttggtttg tgaaataggg ttttaaatga
     7741 aagtgtcagc agggttagaa taaagatagc gataatgctg ttatcagaga aaagaaaact
     7801 gagttcaaac ccaatttgta cgcctaacat catcgcaagt gaccaaatca acacattttt
     7861 tcgattgatt tttatctgtt gatggtggaa taagcataaa ttgatagtgg ctgaaccaat
     7921 cacaatggta agagagcagg cggaaatcac ttggagaggc agttcaggaa acagtgaatg
     7981 taaaatcggc atcattaaaa cacccccgcc aatcccaaat agtgcagaag tgatattgac
     8041 tataatgcca caaatgatca aaataattat cacggtcatt gtcataatcc atctcctaaa
     8101 gtg
//
LOCUS       DANLXF010000103         8061 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.103, whole genome shotgun sequence.
ACCESSION   DANLXF010000103 DANLXF010000000
VERSION     DANLXF010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8061
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.103"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(52..1092)
                     /locus_tag="PXD66_001492"
     CDS             complement(52..1092)
                     /locus_tag="PXD66_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL6319784.1"
                     /translation="MQTTLSPQQIAKKHRTIEKKRSFLLLFFLLICLLSLIFDIMTGP
                     SLLPFNEVWKALFQLGEIEETTKVIVYELRLPMAIMALVVGAALGVGGAEIQTLLNNP
                     MASPYTLGLAAAAGFGASIVIAFGGFGLPTEISVPVGAFVMTLFSSAILFAFSSLGRF
                     SSAILVLVGIALLFLFQSLLSLVQFISAPEVSQQILFWLFGNLTKATWQTISIAFGVT
                     TVCILLLFKDAWKLTALRLGEARALSLGINLTWLRMKTLLIVSVMTATCISFVGIIGF
                     IGLVAPHIARMLLGEDQRFFLPGSMLVGAAFLSMSSVLSKSLVPGALFPVGIITALVG
                     VQFFFWIVLAKR"
     gene            complement(1181..1771)
                     /locus_tag="PXD66_001493"
     CDS             complement(1181..1771)
                     /locus_tag="PXD66_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein phosphodiesterase"
                     /protein_id="HDL6319785.1"
                     /translation="MNFLTHSLISLEIDTQTKQQTLYGNFAGDFYKGLIPHLNLADNL
                     KSGVVLHRSIDKISDRPDSLTTQLLQGTFGRYKGIVSDIMIDHFLAKHFQRLFAKDLN
                     EVQAQIFQQIEQQRTIFPEQFVPMFNWLQSRKALTHYADLDFLEQVFLGMSSRVKKGE
                     ILRNAVTEIWQNYTLLEEAAIQEFFTVKDRATRELS"
     gene            complement(1772..3145)
                     /gene="modF"
                     /locus_tag="PXD66_001494"
     CDS             complement(1772..3145)
                     /gene="modF"
                     /locus_tag="PXD66_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HDL6319786.1"
                     /translation="MVVDGNGSGKTTFSLALQGKISLYSGDYGNSFQHIQLLSFEQQQ
                     KMLEQIFKDRNNDSVSPDDFGLTARQVILNGSPKEALCEEYAHKLHIQHLLERPFMHL
                     STGESRKVLFCQMLVSEPDLFILDEPFEGLDQQSVQYWQQLIAELQSKMALVLIVNRF
                     NDIPDAANHIELLDNLSLILQGERAEIEQQAVYSQLKFAEENVNVPLPDSAAPLLSLP
                     ENINPFELENVNIQYGEKKILDNLSWTVQPKQHWWIKGPNGAGKSTLLSIITGDHPQS
                     YANKVRLFGKQRGSGETIWDIKKNIGYVSSQLHMDYRVNCSAMEVILSGFFDSIGVYQ
                     QVPNALQLKAMEWLERLNLSNVAKKPFRSLSWGQQRLLLITRAMVKHPSILILDEPLQ
                     GLDGINRKLVKQFIEQLVTNSQTQLLFVSHQDSDAPNCITHLFEFVPKGETNEFGFEY
                     HQTALGA"
     gene            complement(3263..4216)
                     /gene="fmt"
                     /locus_tag="PXD66_001495"
     CDS             complement(3263..4216)
                     /gene="fmt"
                     /locus_tag="PXD66_001495"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDL6319787.1"
                     /translation="MSKLNIIFAGTPDFAAQHLQALLDSEHNVIAVYTQPDKPAGRGK
                     KLQASPVKQLAEAHNIPVYQPKSLRKEEAQAELKALNADVMVVVAYGLILPEAVLNAP
                     KYGCLNVHGSLLPRWRGAAPIQRSIWAGDTETGVTIMLMDVGLDTGDMLHKVTTPIEP
                     NETSASLYAKLAELAPLALLEVLNGLANHTFKAEKQDEALSNYAEKLSKEEAKLDWQL
                     PAVQLERNIRAFNPWPFSFLTLTVDGVEQSIKIHQATVLPHQEKAAGTVINVDKTGIQ
                     IATQQGVLNITQLQPAGKKPMSVQDFLNGRADWFVSGTQLS"
     gene            4331..4942
                     /locus_tag="PXD66_001496"
     CDS             4331..4942
                     /locus_tag="PXD66_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDL6319788.1"
                     /translation="MEFFIPKADTIFEEEIKKSRFITYLRHTEGMEQAKAFWQELKIQ
                     HPHARHWCWATVAGLPNDSQQYGFSDDGEPSGTAGKPMLNYLLGSGLGEITAVVVRYY
                     GGIQLGTGGLVKAYGNGVQQALLQLERIRKVLRKNYRLQCEYEQFNTISHLISEKDIV
                     IVEQLFSDKVDLLLAFNPSEVEAFADELTQRFSGKLALIKEEI"
     gene            4946..6430
                     /locus_tag="PXD66_001497"
     CDS             4946..6430
                     /locus_tag="PXD66_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter"
                     /protein_id="HDL6319789.1"
                     /translation="MQFFSIIRIVGILVMCFSFTMLVPATVALIYGDGGGRAFLEAFA
                     LNFMVGTLLWWLCRDNKNELRSREGFLIVVLFWVVLGLLGAVPFIILENPDLNVAESI
                     FESFSGLTTTGATVITGLDSLPKAILFYRQFLQWLGGMGIIVLAVAIIPLLGIGGMQL
                     YRAEMPGPLKEQKMRPRIAETAKALWLIYLSLTILCAVAFWLAGMTVFDAISHSFSTV
                     SIGGFSTHDASIGYFNNAAISYITVFFLLLSACNFALHFAVIDRIRDAQSRKKGGILS
                     RLYWGDYEFRFFLMVQLGLFLVCTLVLVGYGYFDNTSVTIEQALFQSVSISTTAGFTT
                     NDFSQWSSFLPTLLVLSSFIGGCAGSTGGGLKVIRVLLLYLQSKREIHRYIHPNVIQP
                     IKLGNHVLSERVVDGIWAFFSAYFFVFVICWLATIACGMETFDALNAVIASLNNLGPA
                     LGSVSSNFTQVPDSAKLVLTFAMVCGRLEVFTLLVILSPVFWKD"
     gene            6431..6958
                     /gene="hemG"
                     /locus_tag="PXD66_001498"
     CDS             6431..6958
                     /gene="hemG"
                     /locus_tag="PXD66_001498"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HDL6319790.1"
                     /translation="MKTLILYFTTDGQTKKIAERLADVITHEVELISLKDQAVDFAEK
                     IANADQIVIGASIRYGHFNPLVYQFVEQHYPALNAKKTAFYGVNLTARKDNRNTPETN
                     TYVRKFLAKVKWKPTHIEVIAGALLYLRYKPFDRLMIRFIMKITKGETDTSKEYEYTD
                     WQQVENFGKLLANQL"
     gene            7090..7485
                     /gene="mscL"
                     /locus_tag="PXD66_001499"
     CDS             7090..7485
                     /gene="mscL"
                     /locus_tag="PXD66_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDL6319791.1"
                     /translation="MSILKEFREFAVKGNVVDMAVGVIIGGAFGKIVSSLVSDVVMPP
                     IGWLIGGVDFKDLAIEIAPAKEGAEAVMLKYGAFIQNVFDFLIIALAVFGMVKVINSL
                     KKAPVVEEAPAEPTAEEKLLTEIRDLLKK"
     gene            complement(7598..7861)
                     /gene="rpsT"
                     /locus_tag="PXD66_001500"
     CDS             complement(7598..7861)
                     /gene="rpsT"
                     /locus_tag="PXD66_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDL6319792.1"
                     /translation="MANIKSAKKRAVQSEKRRQHNASQRSMMRTFIKKVYAAVAAGDK
                     AAAQTAFVEMQKVVDRMASKNLIHANKAANHKSKLAAQIKKLA"
BASE COUNT         2231 a         1607 c         1802 g         2421 t
ORIGIN      
        1 taagtttcca cgcttaatag ttacatcgtt taagttgagc atagaaaatc cttatcgttt
       61 cgccaataca atccagaaaa agaactgcac accgactaat gccgtaataa taccgaccgg
      121 aaataatgcg cccggcacaa ggctttttga tagcacggat gacatcgata aaaatgccgc
      181 acccactaac attgaaccgg gcaggaaaaa gcgttggtct tcgcctagca acattcgggc
      241 aatatgcggg gcaactaagc caataaagcc gataatgcct acaaagctaa tgcaggtggc
      301 cgtcattaca gacacaatca gcaatgtttt cattcgtaac caagttaagt taatgcctaa
      361 gctcaatgct cgagcttcgc ccaaacgcaa ggcagttaat ttccacgcat ctttgaataa
      421 taataaaata cagactgtag ttacgccaaa ggcaattgag atagtctgcc aagtagcttt
      481 agttaaatta ccgaatagcc aaaacagaat ttgttgggat acctcaggtg cagaaataaa
      541 ttgcaccaat gacagtaaag actgaaataa aaacaataag gcaattccta ccagcactaa
      601 tattgccgag ctaaaacgtc ctaaagagga aaaggcaaat aaaatggcgg aagaaaatag
      661 tgtcatcaca aaagccccga caggtacaga aatttcggtg ggtaaaccaa atccaccaaa
      721 ggcaatcaca atcgaagcac caaagccggc tgcagctgct aaacccaatg tataagggct
      781 tgccattgga ttattaagca aagtttgaat ttcagctccg ccaacaccaa gtgctgctcc
      841 aaccaccaat gccattattg ccattggtaa gcgtagctca tagacaatca ctttggtggt
      901 ttcttcaatt tcgcctaatt ggaataaggc tttccaaact tcattaaacg gaagcaggga
      961 agggcctgtc ataatatcga aaatgaggct gagtaaacaa atgagtaaga aaaagagtaa
     1021 taaaaaactg cgtttctttt caatagttcg gtgcttttta gctatttgtt ggggagagag
     1081 cgttgtttgc ataaaaatgt aatgtttgag aattattatc agacaggata agagaaaaga
     1141 tcgataaatt caagaatatc caagataccg actaaagtat tcaactaagt tctcgagtag
     1201 ccctgtcttt tacagtaaaa aactcctgaa tagcggcttc ttctaaaaga gtataattct
     1261 gccaaatttc ggttacagca tttcttaaaa tctcaccttt cttaactctt gatgacattc
     1321 ccaagaatac ctgttctaag aaatctaaat cggcataatg agttaatgct tttcgtgact
     1381 gtaaccagtt aaacatcggc acaaattgtt caggaaaaat cgtgcgttgt tgttcgattt
     1441 gctgaaagat ctgtgcttgt acctcattta aatctttagc aaataaccgt tggaaatgtt
     1501 tagcgagaaa atggtcgatc ataatatccg acacaatgcc tttataacgc ccgaatgtgc
     1561 cttggagtaa ttgggttgtg aggctgtccg gtcggtccga aatcttatca atcgagcggt
     1621 gtaacaccac accgcttttt aagttatccg ctaaattgag atgcggaatt aaacctttat
     1681 agaaatcacc ggcaaagttg ccatataggg tctgttgctt ggtttgggtg tcaatttcaa
     1741 gtgaaatcag cgaatgggta agaaaattca tttatgctcc aagtgcagtt tggtgatact
     1801 caaacccaaa ctcgttggtt tcgcctttcg gtacaaactc aaataaatgc gtaatgcaat
     1861 ttggagcatc gctgtcttgg tgcgaaacaa aaagtagttg cgtttggctg ttagtcacta
     1921 attgttcaat gaactgtttg actaatttgc ggttaatgcc gtctaaacct tgtaacggtt
     1981 cgtctagaat cagaattgat gggtgtttca ccattgctcg ggtaatcaaa agtaagcgtt
     2041 gttgccccca cgagagagag cgaaacggct ttttggcaac attgcttaaa ttgaggcgtt
     2101 ccaaccattc cattgctttt aattgcaagg cattcggcac ttgttgatac acgccgatgc
     2161 tgtcaaaaaa gccagaaaga atgacctcca ttgcggagca atttacccga taatccatat
     2221 gcaactggct gctgacatag ccgatatttt tcttaatatc ccaaatggtt tcgccacttc
     2281 cccgttgttt gccaaatagt cggactttat tggcataaga ttgtgggtga tcgccggtga
     2341 taatcgaaag caaggtcgat ttcccagctc cattcgggcc tttaatccac cagtgttgtt
     2401 taggctgaac cgtccaactt aggttatcta aaatcttctt ctccccatat tgaatattca
     2461 cattttccaa ctcaaacgga ttgatgtttt ccggcaggct gagtagcgga gctgcactat
     2521 caggcagtgg cacattgaca ttctcttctg caaattttag ctgagaatag accgcttgtt
     2581 gttcaatttc tgctcgctct ccttgcagaa ttaatgataa attatctaat aactcaatat
     2641 gattggcagc atcaggaata tcgttaaaac gatttacaat cagcactaac gccattttgc
     2701 tttgtagttc ggcaatcagt tgttgccaat attgcaccga ctgctgatcc agcccttcaa
     2761 acggttcgtc taaaatgaat aaatccggct cgctcactaa catttggcaa aatagcactt
     2821 tgcggctttc accggtggaa aggtgcataa acgggcgttc aagcagatgt tgaatatgca
     2881 atttgtgggc atattcctca cataacgcct cttttggact gccatttaaa attacttgtc
     2941 gtgcggttaa gccgaaatca tcgggcgaga cactatcgtt gttccgatct ttgaaaatct
     3001 gctccaacat tttttgttgt tgctcgaaag agaggagctg aatatgttgg aacgaattac
     3061 catagtcgcc ggaatagagc gagattttac cctgtaaagc gagtgaaaaa gttgttttgc
     3121 ctgaaccgtt tccgtctacg accacctaaa agtcattggg ttggatctgt aaggcgtcaa
     3181 tcgtgagttt atgttgttta accagtgaaa atcgggcatt ggttattgta agtgaaggct
     3241 gtgtttgcat atatttcccg ttttaactta gttgtgtccc gctgacgaac caatccgctc
     3301 gaccgtttaa gaaatcttgc accgacattg gttttttacc cgccggttga agttgggtaa
     3361 tattcagcac gccctgttgg gtggcaattt gaatcccggt tttatccaca ttaattaccg
     3421 tgccggctgc tttttcttgg tgaggtaaca ccgtggcttg gtgaattttg atactttgct
     3481 caacgccatc aacggttaag gttaaaaaac taaacggcca agggttaaaa gctcggatat
     3541 tgcgttccag ttgaactgct gggagttgcc aatccaattt agcttcttct ttggaaagtt
     3601 tctcggcgta attggacagt gcttcatctt gtttttccgc tttgaatgtg tggttggcta
     3661 aaccgtttaa cacctcaagc aatgccagag gggcaagttc tgccagtttg gcatacaagg
     3721 aggcggaggt ttcattcggc tcaatcgggg ttgtgacttt atgtaacata tcaccggtgt
     3781 ctaagccaac gtccattagc ataatggtta cgccggtttc tgtatcacct gcccaaatcg
     3841 aacgctgaat cggagctgct ccacgccagc gaggtagtag cgaaccgtgt acgtttaggc
     3901 aaccatattt aggagcattc aatactgctt caggtagaat taagccgtaa gctaccacca
     3961 ccatcacatc ggcatttagg gctttcagtt cagcttgagc ctcttcctta cgcagggatt
     4021 tgggttggta aacaggaatg ttgtgagctt cggccagctg ttttaccggg ctggcttgca
     4081 gttttttgcc tcgcccggca ggtttatccg gctgagtata aaccgcaatc acgttatgct
     4141 cggaatctaa taaggcttgt aaatgttgag cggcaaaatc aggcgtgccg gcaaaaataa
     4201 tattgagttt tgacatattt tttattcgtt caagttatca atgaaagact tattaaacca
     4261 ttaaacagca caaataaaaa gtggcttgaa tatggtatca ttgccacatt caagataaga
     4321 gaatcaaaca atggaatttt ttatccccaa agcggataca atttttgaag aagagattaa
     4381 aaaaagccga tttatcacct accttcgcca tactgagggt atggagcagg ccaaggcatt
     4441 ttggcaggag ctgaaaattc agcatccgca cgcacgccat tggtgttggg caacggtggc
     4501 aggcttgccg aatgattcgc aacaatacgg cttttcggat gatggagaac cgtctggtac
     4561 agcaggtaag ccaatgctca attatttgct tgggtcaggc ttgggggaaa ttacagcggt
     4621 ggtggttcgc tactacggtg gtatccagct tggcaccggc ggcttggtga aagcctatgg
     4681 caatggggtg caacaggcgt tattgcagtt ggagcggatt cgcaaagttt tgcgaaaaaa
     4741 ctaccgcttg caatgtgaat atgagcagtt taataccatt tctcatctca ttagcgagaa
     4801 agatattgtg attgtcgagc aactttttag cgataaagtg gatttgttgc tggcatttaa
     4861 cccgagcgag gttgaggcct ttgcagatga attaacccaa cgcttttcgg gcaagctcgc
     4921 attaataaaa gaagaaatct agtctatgca atttttttcc attattcgta ttgttggcat
     4981 tttggtgatg tgtttttcat tcacaatgct tgtgccggca acggtcgccc taatttatgg
     5041 cgatggcggt gggcgggcgt ttttagaagc cttcgctctg aattttatgg tcggtacgct
     5101 gctttggtgg ctgtgccgtg acaacaaaaa cgagctacgt tcccgtgagg gctttttgat
     5161 tgtagtactg ttttgggtgg tgctgggttt gctcggggct gtgcctttta ttattttgga
     5221 aaaccccgat ttaaacgtgg cggaatccat ttttgaatcc ttttccggct taactacaac
     5281 gggtgcaacc gtcatcaccg gcttagatag cttgcccaaa gcgattttat tctaccgcca
     5341 atttttgcaa tggctagggg gaatggggat cattgtgtta gcggttgcga ttattccgct
     5401 gttaggcatt ggtggaatgc aactttatcg tgcggaaatg cccggaccgc tcaaagagca
     5461 gaaaatgcga cctcgcattg cggaaacggc aaaagcgttg tggctgattt atctcagcct
     5521 gacgattctg tgtgcggtcg cattttggct cgccggaatg acggtgtttg atgccatttc
     5581 gcatagcttt tccacggttt cgattggcgg attttctacc cacgatgcca gcatcggcta
     5641 ttttaataat gcggcaatta gctatattac cgtgtttttc ctgctacttt ctgcctgcaa
     5701 ctttgcattg cattttgcgg tgattgatcg catacgagat gcacaatcac gcaaaaaagg
     5761 gggtatttta agccgcttgt attggggcga ttacgagttt cgttttttct tgatggtgca
     5821 gttagggctt ttcttggttt gtaccttggt gttagtgggt tacggttatt tcgataatac
     5881 ctctgttacg attgagcaag cgttgtttca atcggtgtcc atttccacca cggccggctt
     5941 tactaccaat gattttagcc aatggagttc gtttttaccc acgctgttgg tgctgtcttc
     6001 atttatcggt ggttgtgccg gctcaaccgg tggcggcttg aaagtgattc gggtgttgct
     6061 gctttattta caaagtaaac gtgaaatcca ccgctacatt caccccaatg tgattcaacc
     6121 gattaaatta ggcaaccacg ttttatcgga acgggtggtt gatggtattt gggcgttttt
     6181 ctcggcgtac tttttcgtgt tcgtgatctg ctggcttgct accatcgctt gtggaatgga
     6241 aacctttgat gctctaaatg cggtaattgc ctcactaaat aatctaggtc ctgcattagg
     6301 gagcgtgagc agcaatttca cccaagtgcc ggacagtgcc aagttagtgc tgacttttgc
     6361 aatggtatgc ggacgtctgg aagtgtttac cttgctggtg attttaagcc ctgttttttg
     6421 gaaagattaa atgaaaacct taattctcta ttttacgacc gatggtcaaa ccaaaaaaat
     6481 tgccgaacga ttggcggacg tgattactca cgaagtcgag ctgatttcgc taaaagatca
     6541 agcggtcgat tttgccgaaa aaattgcaaa tgccgaccaa attgtcatcg gtgcttcaat
     6601 tcgctatggg cattttaacc cgttggttta tcagtttgtg gaacagcatt atccggcatt
     6661 aaacgccaag aaaactgcct tttacggtgt gaatttgacc gctcgcaaag ataatcgaaa
     6721 cacgcctgaa accaatactt atgtgcgtaa atttttagct aaagtgaaat ggaaaccaac
     6781 ccacattgag gtaattgctg gggcattact ttacctacgc tataagccat tcgaccgatt
     6841 gatgattcgc tttattatga aaattaccaa aggcgaaacc gatacctcca aagaatatga
     6901 gtacaccgat tggcagcagg tggagaattt tggcaaatta ttagccaatc agttataatc
     6961 aaactgatct aaatcaaaaa aatgtaaatt tatttaaatg ttgtagaaat tttaaagatt
     7021 ttgattataa tcgccagccc acgttcttgc gaagaccgtg tttaatcatt gaatgaaagg
     7081 aaagccccct tgagtatttt aaaagagttc cgtgaatttg cggtaaaagg taatgttgtt
     7141 gatatggctg tcggtgtgat tatcggcggt gcattcggta aaattgtttc ttcattagtt
     7201 agcgatgttg taatgccacc tatcggttgg ttaatcggtg gcgtggattt caaagattta
     7261 gccattgaaa tcgccccggc aaaagaaggt gcggaagcag tgatgttaaa atacggtgca
     7321 tttatccaaa acgtatttga tttcttaatt atcgcacttg cggtattcgg tatggtgaaa
     7381 gtgattaact cactgaaaaa agctccagtt gtagaagaag ctcctgcaga gccaacagcg
     7441 gaagagaaat tattaactga aatccgtgat ttattgaaaa aataattaac tgattttgaa
     7501 acaagggcta ttttagaata tctagaatag ccctttgtgt tatggttaaa aagaaaaaaa
     7561 ccaacataaa gtcggttttt ttcatatctg ttcgatatta cgctaatttt ttgatttgcg
     7621 ctgctaattt agatttgtgg ttagccgctt tgttagcgtg gattaagttt ttagatgcca
     7681 tacggtctac aactttttgc atttcaacga aagccgtttg agctgctgct ttatcgcctg
     7741 ctgctactgc tgcatatact tttttgatga atgtacgcat cattgagcgt tggcttgcgt
     7801 tgtgttggcg gcgtttttca gattgaaccg cacgtttttt tgctgacttg atattagcca
     7861 aggtcaaact cctaaaatat ctgtgtaaaa attagatata aacaaaatcg ttgtttatat
     7921 tgcctatcga tagtttttaa ttcattttca acgagacacg tgcttgaaaa taacaccgtt
     7981 tttctgatag aaaacgatag ggcgagattt taacagtttt tttctgattt tcctataaca
     8041 aactgctaaa attatgatcg a
//
LOCUS       DANLXF010000104         8059 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.104, whole genome shotgun sequence.
ACCESSION   DANLXF010000104 DANLXF010000000
VERSION     DANLXF010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8059
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.104"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            1..971
                     /locus_tag="PXD66_001501"
                     /pseudo
     CDS             1..971
                     /locus_tag="PXD66_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009500034.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(1019..1534)
                     /locus_tag="PXD66_001502"
     CDS             complement(1019..1534)
                     /locus_tag="PXD66_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HDL6319793.1"
                     /translation="MQKKSLISRLLKNIFLYGSLFIIISLVVDWYRSPSAPAQFEQTV
                     HYDIQHQPKVIAQLSHEKPMLLYFWGSWCHYCQVVSPNIQQLANDGTEVLGVALKSGD
                     ENDVQAYLNENGYTFATLNDPSGDFSRGWNIQATPTILIIKDGKIAHHTTGYTSYLSL
                     KFRLWLADVLY"
     gene            complement(1600..2229)
                     /locus_tag="PXD66_001503"
     CDS             complement(1600..2229)
                     /locus_tag="PXD66_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL6319794.1"
                     /translation="MALDFNSVGLDSVEAKGGYGIGLQIGQQLLGSGMDVNAETVARG
                     IYDVLNNNEPAIDINEVTAALQELGQRAEAAQAEAFKAIDAENKAFLEENKKANGVIV
                     TDSGLQYEILTEGTGEKPTATSTVRVHYTGSLIDGTVFDSSVKRGQPAEFPVNGVIRG
                     WTEALQLMPVGSKWRLTIPQELAYGERGAEASIPPFATLVFEVELLDIL"
     gene            complement(2377..3006)
                     /locus_tag="PXD66_001504"
     CDS             complement(2377..3006)
                     /locus_tag="PXD66_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDL6319795.1"
                     /translation="MAVSATSFAQTTQPTAAEQMNAVKASVAEKATVAKEATKEKAAE
                     LKAGSAEKMAIAKAKTESFKDSAKNKMDAVKATTAEKTDVIKAKAASASDTMKVKAQE
                     AKAATAEKAATAHETLNSAKTATAEKAASMKAAATEKMSAVKKSQKVNINTANEEQLQ
                     SLTGIGEAKAKAIVEYRKKHGKIKNAAELANIPGIGDATIEKVAPYLTF"
     gene            complement(3180..3743)
                     /locus_tag="PXD66_001505"
     CDS             complement(3180..3743)
                     /locus_tag="PXD66_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5358 domain-containing protein"
                     /protein_id="HDL6319796.1"
                     /translation="MLRKIVLLSTGLLLAACSSNEPQQPEAFPGEFANADYVLSEENA
                     QKWVIASEQAKQCIYPNLTRIQQEHFSKEDSYIFSQYVFFYPLEEIIGEQYVKIIQND
                     EKSMGYAQYLFKKLKNNEAVEALPKKQCATLRTNAKNDLAVVKGQYQSGMIEETNSQE
                     GKSGDKVATNDNKFFFDIIKWGSMLLL"
     gene            3893..6490
                     /gene="mutS"
                     /locus_tag="PXD66_001506"
     CDS             3893..6490
                     /gene="mutS"
                     /locus_tag="PXD66_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDL6319797.1"
                     /translation="MTQDFSTHTPMMQQYLQLKAQNPDILLFYRMGDFYELFYDDAKR
                     AAALLDISLTKRGQSAGEPIPMAGVPYHAVEGYLAKLVALGESVAICEQIGDPATSKG
                     PVERKVVRIVTPGTVSDEALLPERQDNLVAAVYEEKGTFAIATLDMTSGRFLISELAS
                     KEALSAELQRVQPAEILYAEDFSAASILTNYKGLRRRPVWEFELVTAIQLLNRQFGTQ
                     TLAGFGVEKAVVALCAAGCVLHYAQETQRTALPHINAIHLAQHSDTILLDAATRRNLE
                     LTQNLAGGTENTLAAVLDKCVTPMGSRLLKRWIHQPIRDCGKLQARQKAITALIQYER
                     MSDLQPLLQQVGDMERILARVALRTARPRDLTRLRTALAQLPEIAKNSQNLTASIDQL
                     LAQLADFSPLHDLLERAIIENPPQLIRDGGVIAEGYNAELDEWRQLSDGATQYLENLE
                     IRERESTGIDSLKIGFNAVHGYYIQISQGQAHKAPIHYVRRQTLKNAERYIIPELKTY
                     EDKVLKAKGASLALEKQLYDELFDVLMPRLGELQLAAMMLSELDVLTNLAERADSLGY
                     VCPSFSPMRMVNIKGGRHPVVEQVLTEPFIANPVFLNSQRHLLIVTGPNMGGKSTYMR
                     QIALITLMAYIGSFVPAESAEIGPIDRIFTRIGASDDLASGRSTFMVEMTEMANILHQ
                     ATENSLVLIDEIGRGTSTYDGLSLAWACAEWLAKKTQSLTLFATHYFELTGLPNQLKG
                     VANVHLDAREHQDSIVFMHSVQEGAASKSYGLAVAALAGVPKQVIQLAKQRLAHLEEI
                     SHQTKQANEHPQADLLFSDGFDLQKNEAIQPLVVENEVEKALKALQPDELTPRQALDE
                     LYRLKKLLG"
     gene            complement(6538..7992)
                     /locus_tag="PXD66_001507"
     CDS             complement(6538..7992)
                     /locus_tag="PXD66_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL6319798.1"
                     /translation="MKKIVSTLLILTSNLAFAETTPAQLQEKMDNQRMQKNLHTLEKP
                     EPQAKPQAVNAQAHRQSISISSQQLLQQPELLNSAMLTALVTNHTENVQFLLPIYQQL
                     PASKQNSEVVQWAEAVNAKAKQNYSEAIKGYRQLLAKYPENQPIRMQLAVALFENREN
                     EAAEAQFYKLQADKLPQEIANAVNSYLEAIGKQDQWSFQGGLTYLNDPNINNAPNAGT
                     TYGNWTAPKKESAQGVGFHFEADKKWSWGNGFFHEFRLNGNGKYYWNNKKYNEYSLRE
                     SLGLGYQNAKQKITLLPFFEQMWYAGGSSQTESTKRYSNAGGVNARWQYWITPQWQSA
                     ISYEYAEQRYTRRQHLDGNYHFIAPSLYFYPNSRQYWFVGANFNRTSPRDLDDSFIRR
                     GVSIGWGQEWKNGLSTQLSASYARKQYRAPMPIFQITQRNSEYGVQASIWHRALHFKG
                     ITPRLTWQYNKVKSNHSFYSYDKNRVYIEFSHTF"
BASE COUNT         2044 a         1676 c         1878 g         2461 t
ORIGIN      
        1 tttcttttat ttaaaagaga aagggctaga taacgcccaa atcgcttttt tcctttcatt
       61 gctgttgttt tgtaatcagg cattggcaat tttagccggc atttgggggg atcgctacgg
      121 cttggcaaaa atgatgttat tgggctgttt attagatgtg gttgcctata tgctgttttt
      181 agcagcggat aattacctca ttttattgtt ggcaaccact tgctttgggt tggggagttg
      241 cttattcggc actaatgcca gagcctgttt actggcgatt gccggcgatg aatatgctgc
      301 taaaacccgt ttacaaggca aatatctaaa agtcaccagt atgtcttcaa tgctcgcccc
      361 gctgattatt attccgttta ttaggtttga gatgattgaa acgctgattt ggatctgctt
      421 tattttggaa gccggcttgt ttgcgtttat ggtcaagccg ttttattcgg tggaaggcgt
      481 gaatggcggc gtgaaatttc gtttcgcaca aattaaggaa atcatcaaca aagagttttt
      541 gttcgctcac ttaatgctat ttgtgccgct gagtgatttt tccgtattta ttcgacaatg
      601 tcctgcacgc accggagcat tcgccgattg cgttattcat caacggtgtt ttaaccgtca
      661 gtttgcaatc gactttttct cgtaaaatca acctcaccaa acagcaatta atttggattt
      721 cgccgttatt agcggtggcg attattgccc cttggtttat tgcggtaaaa gtcgcaactc
      781 tgaccgctgc ctatatttac accgtgattt ttacggtggt ggaagtgtac gccctcacag
      841 caatggcaaa tttactggtg aaattcgaca acggcaaaaa ccgaggcttt atttttggct
      901 catcgaggct gattttatcg atcaccacaa tgatgacaat gaatttagta ccgcttgtct
      961 ttttggtgta aacaagcggt cggatttagt ggattttttt gcaaatgaaa ggaatcgttt
     1021 aatacaacac gtctgccagc catagtctga attttaagct caaatagcta gtgtagccgg
     1081 tggtgtggtg ggcgatttta ccgtctttga taatcaagat ggttggggtg gcttgaatgt
     1141 tccaacctcg agagaaatca ccgcttgggt cattcaaagt agcgaaagtg tagccatttt
     1201 cattgagata ggcttgtacg tcattttcat cgccggattt cagggcaaca cctaaaactt
     1261 ccgtgccgtc attcgctaat tgttggatat ttggcgaaac cacttggcaa tagtggcacc
     1321 aacttcccca aaaatagagt agcatcggct tctcgtggct taattgggcg attactttcg
     1381 gctggtgttg aatatcataa tgcacggttt gctcaaattg tgccggagcg gagggcgagc
     1441 gataccaatc aaccaccagg ctaataatga taaacaggct gccgtagagg aaaatatttt
     1501 taagtaagcg agatatgagt gattttttct gcataatata gttatcttaa atagcaaaaa
     1561 cctgtgaaat gcctccacag gttttttatt tgagtttgat tagagaatat caagcaattc
     1621 cacttcaaac actaaggtcg caaatggtgg aattgatgct tctgcaccac gttcgccgta
     1681 ggctaattct tgtggaatgg ttaaacgcca ttttgagcca accggcatta attgcaatgc
     1741 ttctgtccaa ccacgaatta ctccgtttac cgggaattct gccggctgac cgcgttttac
     1801 cgagctgtca aacacggtgc cgtcaattaa tgaaccggtg tagtgaacac gcacggttga
     1861 agtcgcagtc ggtttttcgc ctgtgccttc ggttaaaatt tcatattgta aaccgctgtc
     1921 ggtaacgatt acaccgtttg cttttttatt ctcttccaag aacgctttgt tttcggcatc
     1981 aatcgctttg aatgcttctg cttgagccgc ttccgcacgc tgacctaatt cttgtaatgc
     2041 agcggtcact tcattgatgt cgattgccgg ctcattgttg tttaacacat cataaatacc
     2101 acgggcaacg gtttctgcgt ttacgtccat tccgctgcct aaaagttgct gaccaatttg
     2161 taaaccgatg ccgtaaccgc cttttgcttc tactgaatct aatccaactg aattgaaatc
     2221 taatgccatt ttctgttcct tttgagttta ttgaatactg tattgaaaga aatacgattc
     2281 acttaatggg ggtgaaattt aagaatacaa ggtgtgaaat gaacaagcgg ttatttttct
     2341 gcaaaatttt gcaacaaaat aaccgcttgc agaacattag aaagttaaat atggagcaac
     2401 tttctcaatg gttgcatcgc caatgccggg aatgttcgcc agttctgctg catttttgat
     2461 tttaccgtgt tttttgcggt attctacaat cgctttggct tttgcttcac caatgccggt
     2521 aagcgattgt aattgctctt cgttggcagt gttgatattc actttttgtg attttttcac
     2581 tgctgacatt ttttctgttg cagctgcttt cattgaggcc gctttttcag cggttgcggt
     2641 tttggctgaa tttaaggttt catgagcagt tgcagctttc tctgcggtgg cggcttttgc
     2701 ttcctgagct ttcactttca ttgtgtcgct tgcagatgcg gctttcgctt taatcacgtc
     2761 cgttttttct gctgtggtgg cttttactgc gtccattttg tttttagcgg aatctttaaa
     2821 tgattcggtt ttggcttttg ctatcgccat tttttccgca gaaccggctt ttaactccgc
     2881 tgctttctct tttgttgctt ctttagccac agttgctttt tcggcaacag aggctttaac
     2941 tgcgttcatt tgttcagccg cagtaggttg agtggtttgt gcaaatgaag tagctgaaac
     3001 agccattgcc acgcccaatg ctaataatgt ttttaatgct tttattgaaa atccttaaat
     3061 ataaaagagg gaaaataatt gaaaaaaact gtattttaag actgaggctt tagataaaag
     3121 ttcaatcggt aggtataaat ggctcggttg tggttaaaac aaccgagtat tgaggctcac
     3181 tagagaagta acatactgcc ccatttgata atatcgaaga agaatttatt atcattggtg
     3241 gcaactttat cgcctgattt cccttcttgg ctattggttt cttcgatcat accgctttga
     3301 tactgccctt ttaccactgc caaatcattt tttgcattgg tgcgtaaggt cgcacattgt
     3361 tttttcggta acgcttcaac cgcttcgtta tttttcagtt ttttgaataa atattgagcg
     3421 taacccattg atttttcatc attttgaatg atttttacat actgctcccc gataatctct
     3481 tcaagcggat agaaaaatac atattgtgag aaaatataag aatcttcttt ggaaaaatgc
     3541 tcttgctgaa tacgggttaa attggggtag atacactgtt ttgcctgttc gctggcaatt
     3601 acccattttt gagcattttc ttccgaaagc acataatcgg cattggcaaa ttcgcccggg
     3661 aatgcttccg gttgttgcgg ttcattgctg ctacaagccg ccaataataa gcctgtagaa
     3721 agtagcacta tttttcttaa cattctgacc tctgttttaa aaatttggtg cattgtaaca
     3781 aaaaaacagc ggctgaccat tgcaaatttt cagcgaaaaa tgaccgcttg tttttatccc
     3841 ttggctgaat ctgttaaact atcagcaatg atttctcaca ataaatagac tgatgacaca
     3901 agatttctcc acccatacgc caatgatgca acaatatttg caactcaaag ctcaaaatcc
     3961 ggacatacta ttgttttacc gaatggggga tttttatgag ctgttttatg atgatgcgaa
     4021 acgagcggca gcattgttgg atatttcgct caccaagcga gggcaatctg ccggtgagcc
     4081 gattccgatg gcgggtgtgc cttatcacgc agtggagggg tatttggcga aattggtggc
     4141 gttgggggaa tcggtagcaa tttgtgaaca gattggcgac ccagcaacct caaaagggcc
     4201 ggtagaacgc aaagtggtac ggattgttac gccgggaaca gttagcgatg aggcattgtt
     4261 acccgaacgc caagataact tggtggcggc agtttacgaa gaaaaaggca catttgcgat
     4321 tgctacgcta gatatgacct ccgggcggtt tttaatcagt gaacttgcta gcaaagaggc
     4381 tctatccgcc gagttacaac gtgtacaacc ggcagaaatt ctgtatgcgg aagatttttc
     4441 cgctgcctca attttaacga attacaaagg gttacgccgc cgtccggtgt gggaatttga
     4501 gctggtaacg gcgattcagc tgctaaatcg ccaattcggc acgcaaacac ttgccggatt
     4561 tggggtggaa aaagcggtgg tggcactttg tgctgccggc tgtgtcttgc attatgccca
     4621 agaaacgcaa cgcaccgctt taccgcacat taacgcaatt catttggcac aacatagcga
     4681 tactattttg ctcgatgcag ctactcgtcg caatttagag ctgacccaaa acttagcggg
     4741 tggcacggaa aacacgctgg cggcggtgtt agataaatgt gtgaccccaa tggggagccg
     4801 tctgctcaag cgttggattc atcagccgat tcgagattgt ggtaaattac aagcccgaca
     4861 aaaggcgata acagcattga tacaatatga gcgaatgtcg gatttacagc cgttattaca
     4921 acaagtcggc gatatggagc ggattctcgc ccgagtggcg ttgcgtacgg ctcgccctcg
     4981 tgacctcacc cgactacgca ctgcattggc tcagttaccg gaaattgcaa aaaattcaca
     5041 aaatctgacc gctagtatcg atcagctttt ggctcaactg gcggatttca gcccgttaca
     5101 cgatttgctg gagcgggcga ttatcgaaaa cccaccacaa ctcattcgtg atggtggggt
     5161 gattgcagaa ggttataatg ccgagcttga cgaatggcgg caactttcgg acggggcaac
     5221 gcaatatctg gaaaatttgg agatcagaga acgtgaaagc accggcattg attcgctgaa
     5281 aatcggcttt aatgcggttc acggctatta cattcagatc agccaagggc aagcacacaa
     5341 agccccgatt cactatgttc gccgccaaac gctcaaaaat gcggagcggt atattatccc
     5401 tgagctgaaa acttacgaag acaaagtgct gaaagcaaaa ggggcatcgc ttgcccttga
     5461 aaaacagctt tatgatgaac tgtttgatgt gttaatgcct cgcttaggcg aattgcaatt
     5521 agcggcgatg atgctgtctg aacttgatgt cttaaccaac cttgccgagc gagctgatag
     5581 tttaggctat gtttgcccga gctttagccc gatgcgaatg gtgaatatta aaggcggcag
     5641 acacccggtg gtggagcagg ttttaaccga gccgtttatt gccaatccgg tgtttttaaa
     5701 ttcccaacgg catttattga ttgtgaccgg cccgaatatg ggcggtaaaa gcacctatat
     5761 gcggcaaatt gccctcatta cattgatggc gtatatcggc agttttgtgc cggcagaaag
     5821 tgcggaaatc ggcccgattg accgtatttt cacccgaatt ggggcaagcg atgatttagc
     5881 ttcagggcgt tcaaccttta tggtggaaat gaccgaaatg gcgaatattc tgcatcaagc
     5941 caccgaaaac agccttgtgc tgattgatga aatcgggcga ggcacttcta cctatgacgg
     6001 cttatcactg gcgtgggcgt gtgccgaatg gctggcgaag aaaacccagt cactcacttt
     6061 gtttgcaacc cactattttg aacttaccgg cttgccgaat cagctcaaag gggtggcaaa
     6121 tgtgcattta gacgcgcgtg aacatcaaga cagtattgtg tttatgcact cggtgcaaga
     6181 gggggccgcc agcaaaagtt acggcttggc ggtggcagcc ttggcgggtg tgccaaagca
     6241 agtgattcaa ctggcaaagc agcgtttggc acatttggag gaaatttccc accaaaccaa
     6301 acaagcaaac gaacatccac aggcggattt gttgttttcc gatggttttg atttgcaaaa
     6361 aaatgaagca attcaaccgc ttgtagtaga aaatgaagtt gaaaaagccc tgaaagccct
     6421 tcagcctgat gaacttaccc caagacaggc tttggacgag ttgtatcgct tgaaaaaatt
     6481 gttggggtaa attgaaaaaa taactaagaa atcgccccgt ttaaggggcg tttttgttta
     6541 gaacgtatgt gaaaactcaa tatagacacg gtttttgtca taactataga agctatgatt
     6601 gctttttacc ttgttatatt gccacgttaa gcgaggggta atacccttaa aatgcaaggc
     6661 tctgtgccag atgctggctt gcacaccata ttcactgtta cgttgtgtaa tttggaaaat
     6721 cggcattggg gctcggtatt gtttgcgggc gtagctggca cttaattggg ttgaaagccc
     6781 gtttttccac tcttgtcccc aaccaataga aacacctcgg cgaataaagc tatcatctaa
     6841 atcacggggg ctggtgcggt taaaattcgc ccccacaaac caatattgcc ggctgttagg
     6901 gtaaaaatag aggctcgggg caataaaatg ataattgccg tctaaatgtt ggcggcgagt
     6961 gtaacgctgt tccgcatatt cgtagctgat tgcagattgc cactgtggag taatccaata
     7021 ttgccagcgg gcattcacac cgcctgcgtt ggagtagcgt ttggtgcttt cggtttggct
     7081 tgagccaccg gcataccaca tctgctcaaa aaacggcaac aaggtgattt tttgttttgc
     7141 attttgataa cccaagccga ggctttctct gagcgaatat tcgttatatt ttttgttatt
     7201 ccagtaatat ttgccgtttc cgtttaaacg aaactcgtgg aagaaaccat tgccccaaga
     7261 ccacttttta tccgcctcaa agtgaaagcc gaccccttgg gcggattctt ttttcggtgc
     7321 agtccagttg ccataggtcg tgcctgcatt tggagcattg ttgatattcg ggtcattcaa
     7381 ataggttaag ccgccttgaa aactccattg atcttgcttg ccaatcgcct ccaaatagct
     7441 attgaccgca tttgcaattt cttgcggtaa tttgtccgct tgtaacttat aaaattgagc
     7501 ctctgcggct tcattttccc gattttcaaa caatgcaacc gctaattgca tacggattgg
     7561 ctgattttca gggtattttg ccagtagctg acggtagcct tttattgcct ctgaataatt
     7621 ttgttttgct ttggcattaa ctgcctccgc ccattgtaca acttcgctgt tttgtttact
     7681 ggcgggtaat tgttgataaa taggcagtaa gaattgaaca ttctcagtgt ggttagtgac
     7741 caacgcagtt agcattgccg agttgagcaa ctcaggctgt tgaagcagct gttggcttga
     7801 aatagagatc gattgccggt gagcctgtgc attaaccgct tgtggtttgg cttgaggttc
     7861 aggcttttct aatgtatgta ggtttttctg cattcgctgg ttatccattt tttcttgaag
     7921 ttgagccggt gtggtttccg caaatgccag gtttgaggta aggattaaaa gcgttgagac
     7981 tatttttttc ataaagctat cttttatttg aatagaaaag gctatttttc aatagccttt
     8041 agtgaagagt tttgaggtt
//
LOCUS       DANLXF010000105         8039 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.105, whole genome shotgun sequence.
ACCESSION   DANLXF010000105 DANLXF010000000
VERSION     DANLXF010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8039
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.105"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            33..2483
                     /locus_tag="PXD66_001508"
     CDS             33..2483
                     /locus_tag="PXD66_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HDL6319799.1"
                     /translation="MTVEVGGVVITADLEFENLLRDTQKVDKAMDDIGKSTLKAEKGL
                     KGLETQATKTAIAVNQATNKMKNIRGVAGQLGYQIQDISIMLQMGSNASTVIAQQGSQ
                     ILSIFGPMGAVAGAILAISGAIGGALLPSLFDSTDATELLAAAQKNLSEVVTQTESGV
                     SALSAKIIKLAKVSEDAAKAKIAVAMSDAKIAIKAAGDSSQKAFEQFDGFFGSFTNSS
                     QSIRNAIDELKRYESKGKDVGAVIKELGGTYGGAIAEINALDSVTSEMSKTLGITQSE
                     ALGLIKLLAQLEQDKSPDNIKLLAIALSDLSDKNGWANEELNKLTRTINDNSVSALDA
                     ETSVRLLESAIKDLQNAIQNSQGALDGNVAKMNQLADAAERKAATIGKTERETAKYAA
                     TLLATTDEAKKNLDSTLSRIDASYDQIEAYQAEQKALQDKKTADAKAAREAEAAAKKS
                     AQAKKQVVEQLRQMATQYEIAVLRQKGMHLEAVKLEAGMRLGAAATKEQREEAEKLAQ
                     SIYGATAASQNFNAIQAQASPVTALDQRHQQQLAQIEEYKILYPQKIMEAEAARAAIE
                     EQYRQQRMEAQWEEWKQASDGARMFGDAVDAVGSSATSTITGLLNGTMSVRDAFASIA
                     NTILNSVVQSLVEMGMAQVKQMIMGQTAAKASMAAQMVQAKALAASFAPAASMVSLAT
                     QGANAIPAQAAITSTMSMAQVAAVTGRKLGGPVSARQMYRVGENNQPEIFKASNGMQY
                     MIPGQSGRVFSNKQSQGQGGGESKTVAVIVNQTNHFSSDEASANQAAVAKALSNQIKA
                     SVRLELQSQMRAGGVLAR"
     gene            2487..2816
                     /locus_tag="PXD66_001509"
     CDS             2487..2816
                     /locus_tag="PXD66_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6319800.1"
                     /translation="MQTFNFKVEQDYTVQNEPSVVILKFADGYEQTAPKGLNHNLKKY
                     QGITVKANKAEAVKIQQFLNAHGGYKKFNWRDRTSDETVTVRCRSWSSTKLGAVIEFT
                     LNFDEVI"
     gene            2838..3284
                     /locus_tag="PXD66_001510"
     CDS             2838..3284
                     /locus_tag="PXD66_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDL6319801.1"
                     /translation="MASELIKLEQDALIELWEIDLTHIYSSSNPNQRGEIFRFHNGVS
                     QTQQNIWWQGNEYQAYPIAADGFEISGQGPSNRPTLTVSNLYGVVTVLVEDFGQPVEA
                     KVSSEETVDGNIQMTVELLKKMDQIADQRYRRNQLNDLRTGGTLSK"
     gene            3292..4008
                     /locus_tag="PXD66_001511"
     CDS             3292..4008
                     /locus_tag="PXD66_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDL6319802.1"
                     /translation="MPISISNQMKLDLAKLEQNAMLDLYEVDLRKLQDKDGNAGGVYR
                     FYSGLNELKTSVVWQGRTYDPYPIEATGFERNSSGPSNRPTLTLSNLFGLVTGIANQF
                     DECIGAIVRRHQVYAQYLDAVNFAGGNAKADPNQEIISHFVIEQLTSLTRETATFTLA
                     LPIETDNAKIPSRIIMADTCTWIYRSAECGYTGKQYFDEKDKATSDPKADKCSHCLNG
                     CELRGNQRNFGGFVSVNKLG"
     gene            4012..4743
                     /locus_tag="PXD66_001512"
     CDS             4012..4743
                     /locus_tag="PXD66_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL6319803.1"
                     /translation="MELEQQIIDYALQHEPHEMCGFVVFDGNKNRFIPCENQAEDKAN
                     YFEISDLDYIKAEEKGELVAVVHSHPEPNGKPVLSTLDRKMQVQTGLDWWLVHNRQIH
                     KFRNVPHLIGREFKHGTMDCYTLYRDAYMLAGYEMDEFERQDDWWHTGQNLYLDNIQG
                     QGFERVETPQIGDVILMQVGADVPNHAAIYIGEQMVIHHSPNRLSKRDLYDGYWLRHT
                     HSIWRHKLAEKLDFDGILNDIAVNN"
     gene            5012..5602
                     /locus_tag="PXD66_001513"
     CDS             5012..5602
                     /locus_tag="PXD66_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDL6319804.1"
                     /translation="MITVKFYGHLRKFGTDFRLEVKDTAEAIRALCSQLTGLRETLRD
                     GIYKVRIGKQYLDASALEKGLFYCLKKGQTIHFTPVIKGAKSGGVFQAVLGVALIGAA
                     FFLGPVGWGVLGASSAMMMGAMGASMLLGGVAQMLTKMPNAPNIGNEKEKENSTSFSN
                     LNNLVAQGKPVPLAYGLIRTGSLVISQGVETMTVEV"
     gene            5605..>8039
                     /locus_tag="PXD66_001514"
     CDS             5605..>8039
                     /locus_tag="PXD66_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HDL6319805.1"
                     /translation="MGLFSRKRKNHTPKEAPETGRSKQIVNIVELLCEGEIEGLVDGF
                     KSIYLDGTQIQNDDGTYNFNNVSGQLNVGTQDQEVLEGYDSSQNEVSVGVEVKKKNGA
                     IVRTVTDERISRLRLTLGVRSLFRQNNQGDTNTTNVDLKITIGTRQYSHSFNGKYSSQ
                     YLESVVFDNLPSVPFNISVERVTEDSNSQRLQNGTIWSSYTEIIDTEFTYPNSAVAGI
                     SFDSEYFNNIPTRNYLIKAKKVKLPSNYDPVKRTYTGFWDGTFKVAWTNNPAWEIYDL
                     APILSKMLGVEISFDKWALYDVSRYCDQLVPDGMGGQEPRFTCNVWLTEVKTAYDLLN
                     DFCSVFRAIPIWTGTEVSVIIDRPRDPVWTYTNANVVSGFERSYSARKSRHNAVQVTY
                     SDKTNGYESAIEYVSDDEEIKKHGLNLSQVTAFGCTSRGQAYRTGKWILETEKREKET
                     ITFTVGREGLMHLPGDIIRVADSHYAGTEIGGRVLAINGRKVTLDREISIDNASYFTY
                     INGEATHSSIKIQSVNGKEITLDSTPTGLETYGVWSLSTKQISSGLYRSISIVENADG
                     TNTITALQHEPQKEAIVDNSAHFVETARTLYKAPQINAVEVSTGYDGKLYITGDISSG
                     DGKLTYDIKITKDGNLYQYKKGLSDPNIELSDLPNGDYTVIIYGKNAKGQIVTEKTQT
                     FTIDRPPAPTGVVVTGGLGQITLEWDWVNEVTQTEIFAAETDNFALAKKIAKVTARTY
                     AHTLKGNKVVRYYWLRHTRGINVGPFYQQQGVRGQTAVDLDARLTELNTQLSRNIVNE
                     VFDVAAPARGLELV"
BASE COUNT         2563 a         1630 c         1868 g         1978 t
ORIGIN      
        1 ttttttattt aaataatttt aaggaattgt ttatgacggt cgaagttggc ggagttgtaa
       61 ttaccgcaga tctagaattt gaaaatctgc taagagatac tcaaaaagtt gataaagcga
      121 tggatgacat cggaaaatca acattaaaag cagaaaaagg gctgaaaggg ctagaaacgc
      181 aggcaaccaa aacggcgata gcagtaaatc aagccacaaa caaaatgaaa aatatccgtg
      241 gagtagcagg tcaacttggt tatcaaattc aagacatctc aataatgctt caaatgggtt
      301 ctaacgcttc aaccgttatt gctcagcaag ggtcgcaaat cttaagtatt ttcggtccaa
      361 tgggagctgt tgcaggggcg atcttagcta ttagtggtgc tattggtggg gcgttactcc
      421 ctagtttgtt tgattcaact gatgcaactg agcttttggc agcagctcaa aaaaatctca
      481 gcgaagtagt tactcaaacg gaaagtggtg tttccgccct ttctgcgaaa atcattaaat
      541 tagcaaaagt aagcgaagat gcagcaaaag caaaaattgc agtagctatg agtgatgcaa
      601 aaatagctat taaggcagca ggggattcat cacaaaaggc atttgagcaa tttgacggct
      661 tttttggatc gtttacaaat tcaagtcaaa gtattcggaa cgcaattgac gaattaaaac
      721 gctatgaatc aaaaggtaaa gatgttggtg ccgtaataaa agaacttgga ggcacatatg
      781 gtggtgcaat tgccgaaatt aatgcgctgg attctgtgac aagcgaaatg tctaaaacgc
      841 ttggtattac tcaatctgag gctcttggtt tgataaaact gttggctcaa ttagagcaag
      901 acaaaagccc tgataatatt aaattactcg cgatagcttt atctgactta agcgataaaa
      961 atgggtgggc gaacgaagag ctaaataagc taaccagaac gataaacgat aattcagtat
     1021 ctgctttaga tgccgaaaca tcagtaagac tcttagagtc tgctataaaa gacttgcaaa
     1081 atgcaatcca aaactcccaa ggtgcattgg atggcaatgt agcaaaaatg aatcaacttg
     1141 cagatgcggc agaaagaaaa gctgcaacga taggcaagac tgagcgtgaa actgctaaat
     1201 atgcagctac gctattagct acaaccgatg aagctaaaaa aaatcttgat tccacattaa
     1261 gtaggattga cgcatcatac gatcaaatcg aagcctatca agccgaacaa aaagccttac
     1321 aagataaaaa gaccgcagat gcaaaagctg ccagagaagc tgaagcggct gcaaaaaaat
     1381 ccgctcaagc caagaagcaa gttgtcgaac agttgcgaca aatggctaca cagtacgaga
     1441 ttgctgtttt gaggcaaaag ggaatgcatc tcgaagccgt aaaattagaa gctggtatgc
     1501 gtttaggagc tgcggcaaca aaagagcaac gagaagaagc tgaaaaacta gcacaaagta
     1561 tttatggagc aaccgcagca agtcagaact ttaacgctat acaagcccaa gcatctcctg
     1621 ttactgcatt agaccaacga catcaacaac agctagctca gatcgaagaa tataaaatct
     1681 tgtatccaca aaaaataatg gaagcagaag ctgcgagagc tgcaatcgaa gaacaatacc
     1741 gccagcaacg aatggaagct caatgggagg agtggaagca agcgagcgac ggggcgagaa
     1801 tgtttggtga tgcggttgat gctgttggtt caagtgcaac aagcacaatt acggggctac
     1861 taaatggcac aatgtccgtg cgtgatgctt tcgcatcaat tgcaaatacg atcttaaata
     1921 gtgtagtaca aagccttgtc gaaatgggta tggctcaagt taagcaaatg attatggggc
     1981 aaactgctgc caaggcttct atggcggcgc aaatggtgca agctaaagcg ttagctgctt
     2041 catttgcacc cgcggcctct atggtttctc ttgccacaca gggggcgaat gctattcccg
     2101 ctcaagctgc gatcacgtca actatgagta tggctcaagt cgccgcggtg acaggtcgta
     2161 aacttggcgg ccctgtatca gctagacaga tgtatcgtgt cggcgagaac aatcagcccg
     2221 aaatcttcaa agcgagcaat gggatgcaat atatgatccc cggtcaaagc ggtcgtgtat
     2281 tcagcaacaa gcagtcgcaa ggtcaaggtg gaggcgaaag caaaacagta gctgtcatcg
     2341 taaatcaaac caatcacttt agcagcgatg aagccagtgc aaatcaagcg gctgttgcaa
     2401 aagcattgag caatcaaatc aaggcttccg tgagattgga attgcagtca caaatgcgag
     2461 ctggtggagt tctagcgagg taatcaatgc aaacatttaa tttcaaggtt gagcaagact
     2521 acactgttca aaatgagccc agtgtcgtca tactaaaatt tgccgatggt tacgagcaaa
     2581 ccgctcccaa agggttaaac cacaatctga aaaaatacca aggcattacc gtaaaagcga
     2641 acaaagcaga agcggtgaag attcagcaat ttctcaatgc tcacggtggg tataagaaat
     2701 tcaactggcg cgatagaaca agcgatgaga cggtaacggt tcgttgcaga agctggagct
     2761 ccactaaatt aggtgcagtc attgagttta cacttaattt cgatgaggtg atttaatgcc
     2821 aaaagccccc caaaaaaatg gcatcagagt tgataaaact cgaacaagat gccctgattg
     2881 agctgtggga gatagatctc actcacatct actctagctc aaacccaaat cagcgcggag
     2941 agatattccg ctttcacaat ggcgtgagcc aaacacaaca gaacatctgg tggcaaggca
     3001 atgaatacca agcatacccg attgccgccg atggttttga gatttcgggt caaggaccgt
     3061 caaatcgtcc aacactcact gtctctaacc tgtacggtgt tgtcacggta ttagtagaag
     3121 atttcgggca acccgtagag gcgaaagtca gcagtgaaga gactgtagat ggcaatatcc
     3181 aaatgacggt ggaactgctg aaaaaaatgg atcaaatcgc cgatcaacgc taccgtagaa
     3241 atcagttaaa tgatttaaga acaggtggaa cattatctaa ataggtgaag tatgccaatt
     3301 agtatttcta accaaatgaa actcgacctt gccaaacttg agcaaaatgc aatgcttgat
     3361 ttgtacgagg tagatttgcg taaattgcaa gacaaagacg gcaatgccgg tggggtttat
     3421 cgtttttact cgggtttaaa tgagctgaaa accagcgtag tttggcaagg gcggacttac
     3481 gacccttacc ccatcgaggc aacaggattt gagcgtaaca gcagcggccc aagcaatcgc
     3541 cctactctta cgctatcaaa tctgtttggc ttagttacgg gtattgctaa tcagtttgat
     3601 gagtgtatcg gggcgattgt tcgccgtcat caggtttacg cccaatattt agatgccgtg
     3661 aattttgccg ggggtaatgc gaaagctgat ccaaatcagg aaataatcag tcattttgtg
     3721 attgagcagc ttaccagcct cacccgagaa acggcgacct ttacgctggc attaccgatt
     3781 gaaactgata atgccaaaat ccccagcaga attattatgg cggacacctg cacttggatt
     3841 taccgctcgg cggaatgtgg ctacaccggc aagcagtatt ttgatgaaaa agacaaggca
     3901 acatcagacc caaaagccga taaatgcagc cattgcctga acggttgtga attgcgaggc
     3961 aatcagcgta attttggtgg atttgtatcg gtgaataaat tggggtaatg aatggaattg
     4021 gaacaacaaa taatcgacta cgccctccaa cacgaaccgc acgaaatgtg cggctttgtt
     4081 gtttttgacg gcaataaaaa tcgatttatc ccttgcgaaa accaagcgga agacaaagcc
     4141 aattactttg aaatctccga tcttgattac atcaaagcag aagaaaaagg cgaactggta
     4201 gcagtagtgc attcgcaccc tgagccaaac ggcaagccgg tactctctac ccttgatcgc
     4261 aaaatgcagg tacagaccgg tttagattgg tggctagtgc ataatcggca aatccataaa
     4321 ttccgcaatg taccacattt aatcgggcgt gagtttaaac acggcacaat ggattgctac
     4381 acgctctacc gtgatgccta tatgcttgcc ggttacgaga tggacgagtt cgagcgacaa
     4441 gatgactggt ggcataccgg gcaaaatcta tatttggata atattcaagg gcaaggtttc
     4501 gagcgagtag aaaccccaca aatcggcgat gtgattttaa tgcaggttgg agctgatgta
     4561 cctaaccacg cagcgattta tattggcgaa cagatggtaa tccatcacag cccgaaccga
     4621 ttatctaagc gtgatttata tgatggctac tggttacgcc atacccacag tatttggcgg
     4681 cataaactgg cggagaagtt agattttgat ggcatattga atgatattgc tgtaaataac
     4741 taacaaaaaa tgaccgcttg taacaaaaac aaaccccgaa gcgtttgcag cacttcgggg
     4801 tttttcatat ccacttacct aactaagagg acataaatta aatgaatgat aaacgattta
     4861 cattcaaatt tctaggagtt cttatggaag caattaatat taccccaaaa gaaatcagaa
     4921 aaacaatgtg gacagccgca ataattctct ttttgtttgc gttcatctgg aaagcccctg
     4981 aatttatcac tgctattcgt tggtggtaac tatgattaca gttaaatttt acggacatct
     5041 tagaaaattc ggtacagact ttagacttga agttaaagac accgcagaag ccatcagagc
     5101 gttatgctca cagcttacag gcttgcgtga aacgttgcga gacggtattt ataaagtccg
     5161 catcgggaaa cagtatttag atgcctcagc ccttgaaaaa gggctttttt attgcctgaa
     5221 gaaagggcaa accattcatt ttacccctgt tatcaaaggg gcgaaaagcg ggggtgtttt
     5281 ccaagctgtg ttaggtgtag ccttaattgg agccgcattt tttctcgggc cggtcggttg
     5341 gggagttcta ggggcttctt ccgcaatgat gatgggagca atgggggcat caatgttgct
     5401 tggaggagtt gctcaaatgc tcaccaaaat gccgaatgcc ccgaatatcg gtaatgaaaa
     5461 agagaaagaa aactcaacgt ctttttcaaa tttaaataac ttggtggctc aaggcaaacc
     5521 tgtgccgttg gcttatggct taattcggac cggctcgctt gtgatttcac agggagttga
     5581 gacgatgact gtagaggtat aattatggga ttatttagtc gtaagagaaa aaatcatact
     5641 cctaaagaag caccggaaac cggtcgctct aaacagattg taaatattgt ggaattgctg
     5701 tgcgagggcg aaattgaagg cttggtagat ggctttaaat cgatttactt agacggcacg
     5761 cagatccaaa acgatgacgg cacctataat tttaataatg tgagcggtca gctaaatgtc
     5821 ggtacgcaag atcaagaggt attagaaggt tacgacagct cacaaaatga agtgagtgtg
     5881 ggtgtagaag tcaagaaaaa aaacggtgca atcgtccgca ccgttactga tgaacgcatc
     5941 agccgtttgc gtttaacgtt gggcgtgcgg tcactattcc gtcaaaataa tcaaggcgat
     6001 accaatacca ccaatgttga tttaaaaatc acgattggca cacggcaata ttcgcatagc
     6061 tttaacggca aatacagctc gcaatacctt gaatctgtgg tgtttgataa cctgccgtct
     6121 gtaccgttta atatctcggt tgaacgtgta acagaggata gcaattctca acgactacaa
     6181 aacggtacaa tttggagcag ctataccgaa attatcgaca ccgaatttac ttaccctaat
     6241 tcagcggttg ccggtatcag ctttgattcg gagtatttta ataatatccc gacccgaaac
     6301 tacctaatta aagcgaaaaa agtcaaattg ccaagcaatt atgatccagt taaacgcact
     6361 tacaccggct tttgggacgg cacatttaaa gttgcgtgga cgaacaaccc tgcttgggaa
     6421 atttacgatt tagctccgat tttaagcaaa atgcttgggg ttgaaatcag ttttgacaaa
     6481 tgggcgttgt atgatgtttc ccgctattgc gatcaacttg tgccagatgg tatgggcgga
     6541 caagagcctc gttttacctg taacgtatgg ctcacggaag ttaaaactgc ctatgacttg
     6601 ttgaatgatt tctgctcggt attccgtgcg attccgattt ggaccggcac ggaagtctcc
     6661 gtgattatcg accgtcctcg tgatccggtt tggacttata ccaacgctaa tgtagtcagc
     6721 ggttttgaac gctcatattc tgcccgcaaa tctcgccata atgcggtaca agtgacctat
     6781 tcggataaaa cgaatggcta tgaaagtgcg attgagtacg tttcagacga tgaagaaatc
     6841 aaaaaacacg gcttaaattt aagccaagta actgcctttg gctgtacatc tcgtgggcaa
     6901 gcataccgta ccggtaaatg gattttagaa accgaaaaac gagaaaagga aaccattacc
     6961 tttaccgttg gacgtgaagg cttaatgcac ttgccgggcg atattatccg tgttgccgac
     7021 agccattatg ccggtacgga aattggcgga cgtgtactgg cgattaatgg gcgaaaagtt
     7081 acccttgacc gtgaaattag cattgataac gccagttatt ttacctatat caacggtgag
     7141 gcaacgcact ctagcattaa aatccaatcg gtaaacggta aagaaattac gctggatagt
     7201 acaccaaccg gcttagaaac ctacggtgtt tggtcgttat cgactaaaca aatcagctct
     7261 ggattatatc gctcaatctc gattgtcgaa aatgcggacg gcacaaacac tatcacagcg
     7321 ttacaacacg aaccgcagaa agaggcaatt gttgataact ccgctcactt tgtggaaacg
     7381 gctagaacac tctacaaagc tccacaaatt aacgcagtgg aagtctcgac cggttatgac
     7441 ggtaagctct acattaccgg tgatattagc tccggtgatg gcaagctaac ctatgatatt
     7501 aaaattacca aagatggcaa tttatatcaa tacaaaaaag gcttaagcga tccgaacatt
     7561 gaactaagtg atttacctaa cggcgattat accgtcatta tttacggcaa aaacgccaaa
     7621 gggcaaatcg tcaccgaaaa aacacaaacc tttactattg atcgacctcc agctccgacc
     7681 ggtgtagtag taacaggtgg attagggcaa attaccctcg aatgggattg ggtaaatgag
     7741 gttacgcaaa ccgagatttt cgcggccgaa accgataatt tcgcccttgc aaaaaaaatc
     7801 gcaaaagtga ccgctagaac ctacgctcac acactaaaag gtaacaaggt tgtacgctat
     7861 tattggctac gtcataccag aggcattaat gtcggaccgt tttatcaaca acagggggta
     7921 agaggacaaa cagcggttga tttggatgct cgattaaccg aattaaacac tcagttatca
     7981 cgcaatattg ttaatgaggt ttttgatgtt gctgcaccgg caagaggttt agaattagt
//
LOCUS       DANLXF010000106         8011 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.106, whole genome shotgun sequence.
ACCESSION   DANLXF010000106 DANLXF010000000
VERSION     DANLXF010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8011
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.106"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            73..720
                     /locus_tag="PXD66_001515"
     CDS             73..720
                     /locus_tag="PXD66_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HDL6319806.1"
                     /translation="MSIFQQTNPNRRRYGLAVFIGIIAGIISAFVKWGAEHPFPPRSP
                     IDLFTAACPQPVLDAMNAAADQVAAKTIALQECSRAFLNPPHVFLRDYIGIDPTEAAF
                     TFADHAFNWIGVTHMIFSLVFAIGYCIVAEIFPKIKFWQGIGAGIIANICVHYITFPA
                     LGLTPPVAEWPLYEHISELVGHIFWFWTIEVIRRDLRNRITREPDAEVPLDQPFR"
     gene            complement(853..2229)
                     /gene="trkA"
                     /locus_tag="PXD66_001516"
     CDS             complement(853..2229)
                     /gene="trkA"
                     /locus_tag="PXD66_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HDL6319807.1"
                     /translation="MKVIILGAGQVGSTLAENLVSEDNDIVLVDNDPTRLDKLKDKHD
                     LQVIRGEYASPQTLRDAGAGDADLLVAVTNSDEVNMIACQIAYTLFNVPTKIARIRSA
                     DYVRERERLFNDDVLPIDHIIAPEILVKEDILRLIHYPGALQITHFADELVSIVNVKA
                     YYGGPLVGYPISALRDHLPHIDARVVAIFRQDKAIVPQGSTIIEAGDEVFFICATQHI
                     RAVMSELQRLDRPHKRIMIVGGGSIGSSLARDLEDQYRVKIIERNPVRAEKLAEKLSK
                     TMVLTGDASDEELLFEEHIENIDLFLAVTSDDEANIMSALLAKRLGAKKVIILVQRPA
                     YLHLIQGGTIDIAISPQQATISALASYVRKGDILQVSSLKLGVAGAVEIIAHGDATTS
                     KVVGRQIRELKLPQGAIVGAVVRGEEVIIAHKSTMIAENDRVIIFVNDKKQIDEIEKL
                     FQLGVFFL"
     gene            complement(2229..3560)
                     /gene="rsmB"
                     /locus_tag="PXD66_001517"
     CDS             complement(2229..3560)
                     /gene="rsmB"
                     /locus_tag="PXD66_001517"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015431887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDL6319808.1"
                     /translation="MKKQSFKALSSRAISATIILQVLDQGKSLSTLIPDAQKQLEPKD
                     LPLVQEITFGVCRVLPRLESIIKLLVEKPLKGKTRLVHCLLLVGLYQLLYMRVPAHAA
                     VDEVVNATKTLKLDSFRALTNGVLRRFLREQEEILAKVDKHWQTLHPEWLVNKLKKAY
                     PNWREIVEANNQRPPMWIRVNQQHIKTNDYAALISELVEPEFAKNSENPTACVPKCAL
                     LLERAVNVNQLPHFEQGWATVQDAHAQWSAELLGAENGETVLDACAAPGGKTTHILEK
                     APKAKVMALDIEESRLSRVRENLVRLGQIAQVICGDASKPEEWLEEGVLFDRILLDAP
                     CSATGVIRRHPDIKWLRKEGDIAELATLQGEILKALWQRLKPNGILLYATCSVLPQEN
                     SEQIQRFLQDTPNAKQLEIDFNGEKVLEKQFFPQPNGGDGFFYAKLQKVAE"
     gene            complement(3612..4037)
                     /locus_tag="PXD66_001518"
     CDS             complement(3612..4037)
                     /locus_tag="PXD66_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL6319809.1"
                     /translation="MYNKLLIAIDLADLKSAKYVVDTALQMTAKNPNAVYRVVSIIEP
                     VDNSLISAFLPKNFDKEVVAEANQKLHEFTKNHFPEGSKVQHIVAYGTIYEEICRIAD
                     EKSVDLIMMLASSKPNAKGLSSNTVKVARNTTKPILVLR"
     gene            complement(4130..6778)
                     /locus_tag="PXD66_001519"
     CDS             complement(4130..6778)
                     /locus_tag="PXD66_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HDL6319810.1"
                     /translation="MSVESKVDYDDLQDMVASNDSGGRTPSGLAKKAIVYVAILWSVF
                     QIYYASPLPFVFQEWLTHAGINLNVVVDDTKARSIHLAFAMFLAFLSYPAFASSPKHH
                     IPKIDWVFAIVGAGLALYYIFFYEGLVKRFGAPNTQDIIAGCLGILLVLEATRRSLGL
                     PLVIIAIVFLIYNFFGQYFPADWIVSHRSGSLSQIINQQWITTEGVFGVALGVSTKYV
                     FLFVLFGALLDKAGAGNYFIKTAFAYLGHLRGGPAKAAVVSSALTGLISGSSIANVVT
                     TGTFTIPMMKRVGFTKEKAGAVEVASSVNGQIMPPVMGAAAFLMIEYVNMPYSQLITH
                     AFLPALISYIALVYIVHLEACKMNLQGLPRAEEPSPILVFLLRTVASILVIVGLAIGV
                     YYGLGWIRDVAEDYAFMIVCAFLGLWYLLAIRRVASYPDLEADDPNSEIVSLPKRKPT
                     VNAGLHYLIPVVVLLWCLMVEMLSPGLSAFWGTITLIFIQLTQRPLLNFFRKESVTKE
                     IIKQGVRDVVDSLEAGGRNMTGIALATATAGIIVGVVALTGFGVQLSSVIELLSMGNI
                     VLMLILVAVFSLILGMGLPTTANYIVVSSLMATVIMEVGRQNGLIVPLIAVHLFVFYF
                     GIMADVTPPVGLASFAAAAISGGSPINTGMVAFKYSLRTVILPFLFIFNTDLLLIDLR
                     YPGHGWVVFITATVGVLAFTSATMQYIVTKNKWWETALLVVAAFALFRPGFFMDRIYP
                     SERHIEPVQFEEQLLASKVGQNVTLKVEGLNPYGKEIEFYAQLPVPEGATGEERLKNL
                     GLTLIQTDEKIKVDGVETPKVVIDMVEMDSPAAKAGLNWDQTIHYIAVPQDAPSKDWI
                     FIPALLLVFGIVANQRRRVKKQCVIA"
     gene            complement(6980..7942)
                     /locus_tag="PXD66_001520"
     CDS             complement(6980..7942)
                     /locus_tag="PXD66_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HDL6319811.1"
                     /translation="MKKLVKLSFLASLVMGTASVQAAETFVTIGTGGQTGVYYVVGQS
                     ICQLVNRDSAKTGLKCNAPSTGASVANLNAIAAKEMDMGIAQSDWQYHAYNGSSSFEG
                     KKNDKIRAIFSLHPEPFTVMARTDSGIKSFDDLKAKRVNVGDPGSGTRATMNVILAAK
                     GWTDKDFKVASELKPAEMASVMCDNNLDAITYNVGHPNGALKEAAASCDAKLVPVTGP
                     EIDKLVADHSYYAKATIPGGLYKGSDEPTETFGVYATLVTSADVDADKVYNVTKAVFD
                     NFDRFKRLHPAFEHLKQEEMIKNALSAPLHEGAIRYYKEKGWIK"
BASE COUNT         2308 a         1871 c         1567 g         2265 t
ORIGIN      
        1 tttttgaaat cacattccat aaaatggctt gattaacaca acacaattat cactcaaatt
       61 aggattttca ttatgagtat tttccaacaa accaatccaa accgccgtcg ttatgggcta
      121 gctgtattta tcgggattat tgccggtatt atttcagcat tcgtaaaatg gggggcagaa
      181 catcctttcc cacctcgtag ccctatcgat ctgtttaccg ctgcttgccc gcaacctgtg
      241 ttagatgcaa tgaatgccgc agcagaccaa gtggctgcca aaaccatcgc attacaagaa
      301 tgttcacgtg catttttaaa cccaccacac gttttcttac gtgattatat tggtatcgat
      361 ccaactgaag cagcatttac ctttgctgac cacgcgttca actggattgg tgtcacccat
      421 atgattttct cgttagtctt tgcgattggt tactgcattg tggcggaaat cttcccaaaa
      481 attaaattct ggcaaggtat cggtgccggt atcatcgcta atatttgtgt tcactacatc
      541 accttcccgg cattaggttt aacaccaccg gttgctgaat ggccgttata tgaacacatt
      601 tctgaattag ttggtcatat tttctggttc tggaccattg aggtgattcg tcgcgattta
      661 cgtaaccgta ttacgcgtga acctgatgcc gaagtgccgt tagatcaacc gttccgttaa
      721 tttaaaatta gctcgattta tacaaaagcc gcaatcacgt tgcggctttc ttattttaca
      781 agcggtcaaa ttttcatgaa aatttaccaa acaaaaagca cattgtttca tgtagcaatg
      841 cggagcttgg ctttataaga aaaatacacc aagttggaac agtttttcaa tttcatcgat
      901 ctgtttctta tcgttcacaa aaataatcac acggtcattt tctgcaatca tggtggattt
      961 gtgggcgata ataacttctt cgcctcgcac caccgcccca acaattgccc cttgcggtag
     1021 ttttaattca cggatttgtc gtccaaccac ttttgaagtg gttgcatcgc cgtgagcgat
     1081 aatttctacc gctcctgcaa ccccaagttt gagtgaagaa acttgtaaaa tatcgccttt
     1141 tctaacgtaa ctagcgagag cagaaatagt agcttgttgt ggggaaatgg caatatcaat
     1201 agtgccacct tgaatcaggt gtaaataggc agggcgttgc actaaaataa tcactttttt
     1261 cgccccaagc cgttttgcca gcagggcgga cataatattc gcttcatcat cactggtgac
     1321 ggcaaggaat aaatcaatat tctcaatatg ctcctcaaac agcaactctt catcggaggc
     1381 atcaccggta agcaccatcg ttttagatag tttttctgct aatttttcgg ctcgaaccgg
     1441 attgcgttcg ataattttta cccgatactg atcttctaaa tcccgagcta acgaagagcc
     1501 gatgctccct ccaccgacaa tcataatccg cttatgtggg cgatcaagcc gttgtagttc
     1561 actcattact gctcgaatat gttgggttgc acagatgaaa aagacttcat cacctgcctc
     1621 aataatagtt gaaccttgtg gcacaatcgc tttgtcttgg cggaaaattg ccacgacccg
     1681 agcatcaata tgcggtaaat gatcacgcag ggctgaaatc gggtaaccga ccaaaggccc
     1741 accgtaatag gcttttacat tcacgatact gactaactca tcagcaaaat gagtaatttg
     1801 caacgccccc ggatagtgaa taaggcgtaa aatatcttct ttgactaaaa tttcaggggc
     1861 aataatatgg tcaataggca atacatcatc attaaacaac cgctctcgtt ctctcacata
     1921 atcagcactg cgaatacggg caattttggt cggcacatta aataaagtgt aagcaatttg
     1981 gcaagcgatc atattcactt catcactatt agttacagca actaatagat cagcatcacc
     2041 tgcaccggca tcacgcaagg tttgaggtga agcatattcg ccccgaatca cctgtaaatc
     2101 gtgtttatct ttgagcttgt ctaaacgagt tgggtcatta tccaccaaga caatatcatt
     2161 gtcttcactc accaaatttt ctgccagcgt agaaccaact tgtcctgccc ctaaaataat
     2221 tactttcatt actctgccac tttttgtagt tttgcgtaga agaaaccatc accaccgttt
     2281 ggttgtggga agaactgttt ttctaacact ttttcgccgt taaaatcgat ttcaagttgt
     2341 tttgcattcg gtgtatcttg taaaaaccgt tgaatttgct cgctattttc ttgcggtaac
     2401 accgagcaag tggcatataa caaaatgccg tttggcttta acctttgcca taacgccttg
     2461 agaatctcac cctgtaatgt ggctaattct gcaatatcac cctctttacg caaccattta
     2521 atatcagggt ggcggcgaat gactccggtt gcggaacaag gggcatcaag taaaatgcgg
     2581 tcaaacagca caccctcttc aagccattcc tcaggtttgg aagcatcgcc acaaattacc
     2641 tgtgcaatct gccccagtcg gactaagttt tcacgcactc ggcttaaacg gctctcttca
     2701 atatccaacg ccataacctt tgctttcggt gctttttcta aaatatgggt agttttgcca
     2761 cccggtgcgg cacaggcatc taaaacggtt tcaccatttt ctgccccgag caactccgct
     2821 gaccattgtg cgtgagcatc ttgcacggtt gcccaacctt gctcgaaatg tggcagttgg
     2881 ttcacattta ccgctctctc cagcaataaa gcacactttg gcacgcaagc ggtcggattt
     2941 tctgaatttt ttgcaaattc aggctcaacc agctcgctaa tcaacgccgc ataatcattg
     3001 gtcttgatat gttgttgatt gactcgaatc cacattggtg ggcgttggtt gtttgcttca
     3061 acaatttctc gccagttcgg ataggctttt ttaagtttat tgaccaacca ttcagggtgg
     3121 agcgtttgcc aatgtttatc taccttcgct aagatttctt cttgctctcg cagaaaacgg
     3181 cgtagcacac cgttagtcaa ggctcggaag ctatccagtt ttagcgtttt agttgcattc
     3241 actacctcat caaccgcagc gtgtgccggc actcgcatat aaagcagttg gtataaaccc
     3301 actaacagta agcaatgcac caaacgggtt ttgcctttca gcggtttttc aactaataat
     3361 ttgatgatcg attccaagcg aggcagcaca cggcacactc cgaaggtgat ttcttgcact
     3421 aagggtaaat cttttggttc aagctgtttt tgagcgtcag gaatcaaggt ggaaagtgat
     3481 ttaccttgat ctaacacctg taaaataatg gttgcactta ttgctctaga agaaagtgct
     3541 ttgaacgatt gttttttcat aaataaaaat gccattccga cagaatggca ctattgtaac
     3601 tgaatgcggg attatcttaa aactaaaatt ggcttggtgg tgttgcgagc cactttaacc
     3661 gtattagagc ttaagccctt cgcatttggt ttgctgcttg ccagcatcat aattaaatcc
     3721 accgactttt catcggcaat acggcaaatt tcttcataaa tcgtaccata cgccacaatg
     3781 tgctgcactt ttgaaccttc aggaaaatgg tttttggtaa attcgtgcaa tttttggttt
     3841 gcttcagcca ccacttcttt atcgaaattt ttcggcaaga atgcagaaat taagctgtta
     3901 tccaccggct caataataga cacaacccga taaaccgcat tcggattttt tgccgtcatt
     3961 tgtaatgccg tatctaccac atatttagca ctttttaggt cggctaaatc aatcgcaata
     4021 agtaatttgt tatacatatt tattccgtag gggcaggtcc tgtgcctgcc cgaatattaa
     4081 ctaaaatagc ggacgggcac aggacccgtc cctacatagc atataaatac tatgcaataa
     4141 cacactgttt tttcactcta cggcgttggt ttgccacaat accaaatacc aacaatagag
     4201 caggaatgaa gatccaatct ttagaaggag catcttgcgg tacggcaatg tagtgaatgg
     4261 tttgatccca gtttaagcct gctttagctg ccggagaatc catttctacc atatcaatca
     4321 ccacttttgg tgtttctacg ccatcaactt tgattttttc atcggtttga attagcgtta
     4381 agcctaagtt tttcaaacgc tcttcgccgg ttgctccttc cggtacaggt aattgagcgt
     4441 agaactcaat ttctttaccg taagggttta atccctctac ttttaaagta acattttgac
     4501 ctactttgct tgcaagcaac tgttcttcaa actgcaccgg ctcgatatga cgttcagacg
     4561 gataaattct gtccataaag aagcccggac ggaacaaggc gaaagcggct acaaccagca
     4621 acgccgtttc ccaccatttg tttttagtca caatatattg cattgtagca gaggtaaagg
     4681 cgagaacccc aacagttgcg gtaataaaca caacccagcc gtgccccgga taacgcagat
     4741 caattaataa taaatcggta ttaaaaataa ataagaacgg taaaatgacg gttcttaaac
     4801 tatatttaaa tgccaccata ccggtattaa tcgggctacc gcctgaaatt gctgccgcag
     4861 cgaacgaggc taaaccaaca ggtggagtca catctgccat aatcccaaaa tagaatacga
     4921 ataaatgcac cgcaatcaac ggcacgatta acccgttttg acgaccgact tccatgataa
     4981 ccgttgccat taatgatgac accacaatgt agtttgcagt tgtcggtaag cccattccca
     5041 aaattaagct gaatacggct accaagatca acattagcac aatgttgccc attgatagga
     5101 gttcaatcac acttgaaagc tgaaccccaa agcccgttag tgccacaacg ccgacaataa
     5161 tccctgctgt tgcggtagcg agggcgatac cggtcatatt acgaccgcca gcctcaaggc
     5221 tatctaccac atctcgaaca ccttgtttga taatctcttt ggttacgctc tctttacgga
     5281 agaaattcag taacgggcgt tgggttaatt gaataaaaat taaggtaatg gttccccaga
     5341 atgcggataa gcccggcgaa agcatttcca ccattaagca ccaaagtagc acaaccacag
     5401 gaataaggta atgtaaacct gcatttacgg ttggttttct ttttggtaga gaaacaattt
     5461 ctgagtttgg atcgtccgct tctaaatcag ggtagcttgc cacacggcga atagccagta
     5521 gataccataa gcctaagaaa gcacagacaa tcataaacgc ataatcttct gccacatcac
     5581 gaatccaacc taagccgtag taaacgccaa tcgctaatcc tacgatcact aaaattgaag
     5641 ctacggttct tagcaagaag actaaaatcg ggcttggctc ttcggctctt ggtaagcctt
     5701 gtaaattcat cttacaagct tctaaatgca cgatatacac caacgcaata tacgaaatta
     5761 acgcaggtaa gaaggcgtga gtaatcagct ggctatacgg catattcaca tattcgatca
     5821 tcaagaacgc tgcagccccc attaccggtg gcataatttg accgtttact gatgaggcaa
     5881 cttccaccgc accggctttt tctttggtaa agccgacccg tttcatcatt ggaatagtga
     5941 atgtacctgt cgtcacgacg tttgcaatcg aagagcctga aatcaagccg gttaaagcgg
     6001 aagaaaccac cgccgctttt gccggaccac cacgcaaatg ccctaaatag gcaaaggcgg
     6061 ttttaataaa gtagttacct gctcctgctt tatcgagcaa cgccccaaat aatacgaaca
     6121 ggaacacata tttggtcgaa accccaaggg ctacaccaaa cacgccttca gtggtaatcc
     6181 attgttggtt gataatttga gaaagcgaac ctgagcggtg gcttacaatc caatctgccg
     6241 ggaaatattg cccgaagaag ttatagatca ggaatacaat cgcaataatc acaagcggta
     6301 agccaagact acggcgggtc gcttccagca ccaataaaat ccctaaacag ccggcgataa
     6361 tatcttgcgt atttggcgct ccaaaacgtt tcaccaaacc ttcatagaag aaaatataat
     6421 acaacgctaa cccagctcct acgatggcaa acacccaatc tattttagga atatgatgtt
     6481 taggcgagct ggcaaaagca gggtaagata ggaacgccaa gaacattgca aaggctaagt
     6541 ggatagaacg ggctttggtg tcgtccacca ccacattcaa attaatgcct gcgtgagtaa
     6601 gccattcttg gaatacaaac ggcaatggag aagcataata aatttggaac acagaccaaa
     6661 gaatggcgac atacacaatg gcttttttcg caagaccgga cggggttcgc cccccactgt
     6721 cattggaggc aaccatatct tgtaggtcat cgtaatcaac cttagattca actgacataa
     6781 agactcctca aaacgcaagc ggtcattttc ttattaaatt ttacaaattt cctcaaaaaa
     6841 caaccgcttg ctcgtgaaaa cactgttgat aagttatcaa ttaaaggtga aaatcgataa
     6901 cttatcaaca gtttcaaaaa agcaaaaaac cagcacgcta aaaaacgtgc tgattgaatc
     6961 aaattacaga aaggaaatat tacttaatcc aacctttctc tttatagtaa cgaattgcac
     7021 cttcgtgtaa cggagcagaa agggcgtttt tgatcatctc ttcttgtttt aagtgttcaa
     7081 acgccggatg taaacgtttg aaacggtcga agttatcaaa taccgctttg gttacattgt
     7141 acactttatc tgcatccaca tcggcagaag tcactaacgt tgcatataca ccgaaggttt
     7201 ctgtcggctc atccgaacct ttatataaac cgcccggaat cgtagctttg gcataatatg
     7261 agtgatctgc cactaattta tcaatttcag ggccggttac cggcactaat ttcgcatcgc
     7321 aagaagcggc tgcttctttt aacgcaccat tcgggtgacc tacgttataa gtgatggcat
     7381 ctaagttgtt atcacacatt acagatgcca tttctgccgg tttcaattca gaagccactt
     7441 tgaaatcttt atccgtccag ccttttgccg ctaaaatcac gttcattgtt gcacgagtac
     7501 cggaacccgg gtcgcccacg tttacacgtt ttgcttttaa atcatcgaac gatttaatgc
     7561 ctgaatcggt acgagccatt actgtaaacg gttccgggtg aagcgagaaa atggcacgaa
     7621 ttttatcgtt tttcttacct tcaaatgagc ttgagccgtt ataggcgtgg tattgccagt
     7681 ctgattgtgc aatccccata tccatttctt ttgctgcaat cgcatttaag tttgcaaccg
     7741 atgcccctgt tgaaggagcg ttacatttta aacctgtttt tgctgaatca cggtttacta
     7801 attggcaaat agattgaccc acaacataat aaacaccggt ttgaccgcct gttccgatag
     7861 tcacaaatgt ttcagccgct tgaacagatg ctgttcccat cacaaggctt gcaaggaaag
     7921 aaagtttgac taattttttc atttttaact cctaaaatta aggatcagaa aacaaagatt
     7981 tattgttttg agccactgct cacactacaa g
//
LOCUS       DANLXF010000107         7881 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.107, whole genome shotgun sequence.
ACCESSION   DANLXF010000107 DANLXF010000000
VERSION     DANLXF010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7881
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.107"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            52..4248
                     /locus_tag="PXD66_001521"
     CDS             52..4248
                     /locus_tag="PXD66_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDL6319812.1"
                     /translation="MKTKTFTRSYLASFVTIVLSLPAVASVVRNDVDYQYFRDFAENK
                     GPFSVGSMNIDIKDNNGQLVGTMLHNLPMVDFSAMVRGGYSTLIAPQYLVSVAHNTGY
                     KNVQFGAAGYNPDSHHYTYKIVDRNDYEKVQGGLHPDYHTPRLNKLVTEVVPAAVTNA
                     GTSIKPYLNEERFPMFLRAGSGTQALRGKESNKTTGIAGAYEYLTGGTTLQLSKSSPD
                     HWLDYSSNLYQVSYGPLSTYALPGDSGSGSYAYDMNEKRWVLVGVLNFYNGMDNQFNR
                     SAIIRKDFHEKKFAEDIAGTINNTVQNAQFNWTAQGKSSSLSQSNNVQKLNVDLKDSS
                     IANQNTSLPQENHGKTINFNGKDATIVLKQDIDQGAGALNLNANLTIRPETDQTWQGA
                     GIIVGKDKKVNWQVKNPQGDRLSKLGEGTLYVNGRGQNLGDISVGDGIVILNQQADHQ
                     GRKQAFNTVGIVSGRPTVVLGSADQVNPDNIYFGFRGGRLDLNGNSIAFKRIQNSDKH
                     ARIVNHNRDHISTLIIQGQDPLTSNDLIWGKWASNSPADIYEYTNPYQNKRKDYFRLK
                     GNSRVYYPTNATSNDHWEFLSSNREQAIQKILDAKNLRQRYDTFNGFIGEDASNKTNG
                     ILNVVFDTKTEVNTEQDKLKNIYTMSGGFNLNGELTLKGGTLLLSGHPTPHAYDIKNK
                     HDVVRENDWQDSHFTAKNITVNKMAQLYIGRNVNEVNSHFTATDKAKLNLGFINRSTP
                     SCYDSEYTGTTHCEVQAVISDNIFANLATTAIKGNVKLQNHSQLNLGKANLTGSVQAD
                     QTTHITLANHSHWLNNGTSQIGHLTMEKGSILSLNDKFATTEIPVRFNKMIIQGNLKG
                     NGRINYTANLAKGESDHLQVDGIAEGNFVLAVRNSTTEANPKSSLNLLSLKNSNQEGN
                     KASISLENNYVDLGTYRYVLENRNHNYHLFNPLIPNSTSKEMNATSVSSIPKKESVTN
                     VPTLDKKETEQNLTQLQKDFSAHQLENQKAKQSMINAQSELRRLNSQLNVLQKYVNSR
                     RLGYYTQQAVLEQISIIQNKIKQTQTIFNDANATVKLTDQKLEEAKLALGSVNDLVLI
                     KASAPAMQATNQDTSMMNIIQADWISQYANTALSELSAQANSALQISNSLDRQLFKQS
                     DKFNVWSSVEHQKTEHKSDLYRPYKQQTNLTQLGIQMPIDNGLMFGVALSKNHANAEF
                     NEGVNGKSNLLMASLYGKWQSQQGTFISLDGSYGKAKNQLYLFGENHFTRRISSIGAN
                     IGHQFDLAGVQIQPTIGARYYHFSGQDYTLGGAKISSPNTHFMTYQAGLKASKTFSLD
                     DWKVEPSITTHYVDASNKRLAVNVNNNNINQRFGRYLKTEAGIRFEHNQWQFDLHAGF
                     INGNEIKKQHFAGVKLGYSW"
     gene            complement(4303..5397)
                     /gene="mobA"
                     /locus_tag="PXD66_001522"
     CDS             complement(4303..5397)
                     /gene="mobA"
                     /locus_tag="PXD66_001522"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HDL6319813.1"
                     /translation="MSKTIEERISAVILAGGLARRMNGVEKGLQLFEGKPLISHILAR
                     LSPQVSDIWLNVNRSIEQYQQLYPAFSYYQDSLPDFQGPLSGMLAGFEQIESDYLLFV
                     PCDTPFMPELLLQKLKTALRINNVQIAYAHDGERPHPTFALIHRSVQEDLKAYLGSNQ
                     QQLLAFFQSQKSVAVDFSEQKSAFTNVNTLEDLSRVSQFPVKNLAITGYSGTGKTTLL
                     EKLIPKLTACGIRVGLIKHSHHNVDVDKKGKDSYRLREAGANPTMIVCDERWALMTET
                     KQAVEFSQLIAKFNPQEVDLVFVEGFKHETLPKIQLHRKGIEKPLPELDQWTIATATD
                     YPLERENWLDINNIGGIADFIKNWLENKAS"
     gene            5484..5753
                     /locus_tag="PXD66_001523"
     CDS             5484..5753
                     /locus_tag="PXD66_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1040 family protein"
                     /protein_id="HDL6319814.1"
                     /translation="MKCERLEELLELLGEHWRKNPDLHLIDILQQLSVEVGEPDNFKA
                     LSDEVLIYQLKMRNAGKFEPIPGIKKDYEDDFKTALLRARGILND"
     gene            5817..6455
                     /locus_tag="PXD66_001524"
     CDS             5817..6455
                     /locus_tag="PXD66_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL6319815.1"
                     /translation="MKKLALKTAFVALSALFTFNSTAIAADPTAGKEYIEVRKAPSAQ
                     KEVVEFFSFYCPHCYDFELSYKIPSQIKEKLPSDSKLVQYHVNFLGRQSEDLTRAWAL
                     AMALGAEDKVKTALFEGAQKDAFKSMDDIRSVFLANGITAEQFDSGINSFAVNGLVNK
                     QVQLAEDFQIRGVPAFFVNGQYQLNLEGFADSSSTNDFIKRYVDAVVFLSKK"
     gene            6539..7315
                     /locus_tag="PXD66_001525"
     CDS             6539..7315
                     /locus_tag="PXD66_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDL6319816.1"
                     /translation="MTKLSILNLVPIREGQTAKQAVDSMVKLAKHAESIGIERYWIAE
                     HHNMKNLASSATQLLIQHALSHTEKLRVGSGGVMLPNHSPYIVAEQYGTLETLYPNRV
                     ELGLGRAPGTDMRTATALRRNAQSLPFPDEISELKGYFEGTNSVSAYPAQGLNVPFYI
                     LGSSPESAYLAAERGLPYAFASHFAPRFMDDAVRIYRTHFKPSEVLDKPYVILGVNAI
                     AAETDQEAEQLATTQTQFFLNVVTNAQNYLQPPVESDDKV"
     gene            7325..7579
                     /locus_tag="PXD66_001526"
     CDS             7325..7579
                     /locus_tag="PXD66_001526"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase"
                     /protein_id="HDL6319817.1"
                     /translation="MKAEIVPHFGPVSFQQNALYNQEKPVVRQMTACSLIGSRESIEQ
                     QLKSLKARIEIDEIMAVSYIFDEQKQHQSYTWLKEIADKV"
     gene            7581..7874
                     /locus_tag="PXD66_001527"
     CDS             7581..7874
                     /locus_tag="PXD66_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HDL6319818.1"
                     /translation="MSKTMQQKAADAHNMCKLKGDSMLDNSDRQIAFEAVYDSEEIAV
                     QAVEFFTQKAKSVETEPCDIQSEITPIIDGFLMKMWITFSCQAEVILFQMAVR"
BASE COUNT         2635 a         1559 c         1483 g         2204 t
ORIGIN      
        1 agacagaata gccagaattt tttattactt ataaggataa acttctcatt tatgaagacc
       61 aaaacattta ctcgttctta tcttgcttct tttgtaacaa tcgtattaag tttacctgct
      121 gtagcatctg ttgtacgtaa tgatgtggac tatcaatact tccgcgattt tgccgaaaat
      181 aaaggaccat tttcagttgg ttcaatgaat attgatatta aagacaacaa tggacaactt
      241 gtaggcacga tgcttcataa tttaccaatg gttgatttta gtgctatggt aagaggtgga
      301 tattctactt taattgcacc acaatattta gttagtgttg cacataatac tggatataaa
      361 aatgttcaat ttggtgctgc aggttataac cctgattcac atcactatac ttataaaatt
      421 gttgaccgca atgattatga aaaggttcaa ggagggttgc acccagacta tcatactcct
      481 cgattaaata aattagtaac agaagttgtg cctgccgcag tcaccaatgc aggtacatct
      541 attaaaccct acttaaatga agaacgcttc cctatgtttc ttcgtgctgg ttcagggaca
      601 caagcgctaa gaggaaaaga aagtaataaa acaactggaa tcgctggtgc ttatgaatat
      661 cttactggcg gtaccacatt acaattatct aaaagctccc ctgatcactg gttagattat
      721 tcaagtaacc tttatcaagt aagctatgga ccactttcaa cctatgcact acctggtgat
      781 agtggttcag gttcttacgc ctatgatatg aacgaaaaac gatgggtatt agttggtgtg
      841 ctcaatttct ataatggtat ggataatcaa ttcaaccgct ctgcgattat ccgtaaagat
      901 ttccacgaga aaaaatttgc cgaagatatt gcaggaacaa tcaataatac cgtacaaaat
      961 gcacaattca attggactgc tcaaggtaaa tccagctctc ttagtcaatc aaataatgtg
     1021 caaaaactca acgttgatct aaaagatagt agcattgcaa accaaaacac ttctctgcca
     1081 caagaaaatc acggtaaaac cattaatttt aatggtaaag atgcaactat tgtactaaaa
     1141 caggatattg accaaggtgc aggtgcatta aatctgaacg ctaatctcac tattcgtcct
     1201 gaaacagacc aaacttggca aggtgcaggt attatcgtcg gtaaagataa aaaagtgaat
     1261 tggcaagtaa aaaatccaca aggcgatcgt ttatctaaac tcggggaagg aacactctat
     1321 gtaaatggac gtggacagaa tcttggcgat atcagtgtag gtgatggtat tgtaatactt
     1381 aaccagcaag ccgatcacca aggaagaaaa caggccttta atacagtagg aatcgtaagt
     1441 ggtcgcccca ctgttgtgct aggtagtgca gatcaagtta atcccgataa tatttacttt
     1501 ggatttcgcg gaggtcgttt agacctaaac ggtaacagca tcgcctttaa acgtattcaa
     1561 aacagcgata aacatgctcg tattgtaaac cacaatcgcg atcacatttc taccttaata
     1621 atacaaggcc aagatcctct cactagtaat gatcttatat ggggaaaatg ggcaagtaat
     1681 agcccagcag acatttacga atataccaat ccttatcaaa ataaacgcaa agattacttc
     1741 cgtctgaaag gtaattcgag agtatattat ccaacgaatg ctacaagtaa cgatcactgg
     1801 gaatttcttt ccagtaaccg cgagcaagca atacagaaaa tcctagatgc caaaaactta
     1861 agacagcgct atgacacgtt taatggtttt ataggggaag atgcttccaa taaaactaat
     1921 gggatattaa atgtcgtgtt tgatacaaaa acagaagtaa atacagaaca agataaatta
     1981 aagaatatct acacaatgtc gggaggattt aaccttaatg gtgaactcac ccttaaaggt
     2041 ggtacattgt tgctttctgg tcacccaacg ccacacgctt atgatattaa gaataagcat
     2101 gatgttgtgc gtgaaaacga ttggcaagac agccatttta ctgctaaaaa tatcacggta
     2161 aataaaatgg cacaactcta tatcgggaga aatgtcaatg aagtaaatag tcactttact
     2221 gcgactgata aagccaaact caatttagga tttattaatc gttcaacgcc aagttgctat
     2281 gattctgaat acacaggcac tacacattgt gaagtgcaag cggtcatttc cgataatatt
     2341 tttgcaaatc tagcaacaac cgccattaaa ggtaatgtta aattacaaaa ccatagccaa
     2401 ttaaatttag gcaaagcaaa cctcactggt tctgtacaag ctgatcaaac aactcatatc
     2461 actttagcaa atcacagtca ctggttaaac aatggtacga gccagattgg gcatcttaca
     2521 atggaaaaag ggtcgatcct tagcctaaac gataaatttg ctaccacgga aatcccagtc
     2581 cgattcaaca agatgatcat ccaaggtaat ctaaaaggta atggacgaat taactatacc
     2641 gcaaatttag ccaagggcga atctgatcat ctccaagttg acggtattgc tgaaggaaat
     2701 tttgtccttg ccgttagaaa tagcacaact gaagcaaatc caaaaagctc attaaaccta
     2761 ctaagcttaa aaaatagcaa ccaagaaggc aataaagctt ctatttctct agaaaataat
     2821 tatgttgatc taggtactta tcgttatgta ttagaaaatc gtaatcacaa ttaccattta
     2881 tttaatccat taataccaaa ttcaacctct aaagagatga atgctacatc tgtatcctct
     2941 attccaaaaa aggaatctgt tactaatgtt cctactttag ataagaaaga aactgaacaa
     3001 aatcttactc aactacaaaa agatttttca gcacaccaat tagaaaatca aaaagcaaaa
     3061 caatctatga taaatgctca atctgagcta agacgactca attcacaact gaatgtattg
     3121 caaaaatatg tgaattctcg tcgcttaggt tactatactc agcaggcagt tttagaacaa
     3181 attagcatta ttcaaaataa aattaaacaa acacaaacaa tatttaatga cgctaatgca
     3241 actgtaaaac tcacagatca aaagctagaa gaagccaaat tagctctagg ctctgtaaac
     3301 gatcttgtat taataaaagc ctctgctcca gcaatgcaag caactaatca agatacgagt
     3361 atgatgaata ttattcaagc agattggata agccaatacg ctaacacagc actttctgaa
     3421 ctctcggcac aggctaattc tgctctgcaa atcagtaata gcttagatcg ccaactcttc
     3481 aaacaaagcg ataaattcaa cgtatggagc agcgtcgaac atcagaaaac cgagcataaa
     3541 tcagatttat accgcccgta taaacaacaa accaacctga cccaactggg catacaaatg
     3601 ccgatagata acggtttaat gtttggagtt gcattatcta aaaaccacgc taacgcggaa
     3661 tttaacgagg gtgtaaacgg taaatcgaat ctactaatgg caagcctata tggtaagtgg
     3721 caatctcaac aaggcacttt tatcagcctt gatggcagct acggtaaagc aaaaaaccaa
     3781 ctctacctat ttggtgaaaa ccactttacc cgccgaattt cctctattgg tgctaacatt
     3841 ggacatcaat ttgacctcgc aggagttcaa attcagccaa caataggagc aagatactac
     3901 catttcagcg gccaagacta tacactagga ggagcgaaaa tcagctcacc aaatacccac
     3961 tttatgacat atcaagcggg tctaaaagct agtaaaacct tttcattgga tgactggaaa
     4021 gttgaaccaa gcattacaac ccactatgtg gatgcaagta acaaacgctt agcagtaaac
     4081 gtcaataata acaatatcaa ccaacgtttc ggtcgctatc tgaaaaccga agctggtatc
     4141 cgatttgaac ataaccaatg gcaattcgac ttacacgctg gtttcattaa cggcaacgaa
     4201 atcaaaaaac aacattttgc tggtgttaag ctaggatata gctggtaaga aaaagcccct
     4261 agaactattt ctaggggctt tttctactct taaaaagagt atctaacttg ccttattttc
     4321 taaccaattc ttaataaaat ctgctattcc cccaatatta ttaatatcca accaattttc
     4381 tctctcaagc gggtaatccg ttgcggtggc aatcgtccat tggtctagct ctggcaatgg
     4441 tttttcaatc cctttgcggt ggagctgaat ttttggcaag gtttcgtgtt taaagccttc
     4501 tacgaaaacg agatcaacct cttgcgggtt gaattttgca attaattgag aaaattcgac
     4561 cgcttgtttg gtttctgtca ttaatgccca acgttcatcg cacacaatca ttgtcgggtt
     4621 tgctcctgct tcacgtaggc ggtagctgtc tttgcctttt ttatctacat caacgttatg
     4681 gtgggaatgt ttaatcagcc caacccgaat accgcaagcg gtcaattttg ggattaattt
     4741 ttccaataag gtggttttac ctgtgccgct gtagcctgta attgcaaggt tttttacagg
     4801 gaattggctt actcggctta aatcttccaa tgtattcacg ttggtgaatg ccgatttttg
     4861 ttcggaaaaa tcaactgcca cgcttttttg tgattggaag aacgcaagca attgctgttg
     4921 gtttgagcct aaataggctt tcaaatcttc ttgtaccgaa cgatgaatca acgcaaaagt
     4981 gggatgtgga cgctcaccat catgggcata tgcaatttgt acgttattta tccgcaaagc
     5041 agtttttaat ttttgcaata acagctcagg cataaagggc gtatcgcaag gcacaaaaag
     5101 caaatagtca ctttcaattt gctcaaatcc tgccagcatt ccactgagtg gaccttgaaa
     5161 atcaggcaag ctatcttgat agtaagaaaa agcaggataa agctgctgat attgctcaat
     5221 agaacgattg acattaagcc aaatgtcact aacttgtggt gaaagtcggg ctaaaatgtg
     5281 tgaaataagc ggttttcctt caaaaagttg taaccctttt tctaccccat tcattcgacg
     5341 agctaaaccg cccgctaaaa tcacggctga aatgcgttct tctattgttt tactcacagc
     5401 tttatccctt aaaattttgc aaaaataaga gtaaaagtat attatttcgt ttaatttcca
     5461 acaagttttt taaggggtaa aagatgaaat gcgaacgttt agaagagtta ttagagttac
     5521 ttggcgaaca ttggcgtaaa aatcctgact tacacctcat tgatattttg cagcagcttt
     5581 cagttgaagt gggcgagcct gataatttca aagcgttaag cgatgaagtg ttaatctatc
     5641 agcttaaaat gcgaaatgca ggcaaatttg agcctattcc cggcataaaa aaagattatg
     5701 aagatgattt taaaacggct ttattgcgag ctcgtggaat tttaaacgat taagattgtc
     5761 ttatccgtta gctagtttag catttattat ttatcaacat ttttgaagga atcattatga
     5821 aaaaattggc attaaaaacg gcatttgttg cactttctgc cctttttaca tttaactcaa
     5881 cggcaatcgc tgctgatcca accgcaggca aagaatatat cgaagtgcgt aaagcacctt
     5941 ctgcacaaaa agaagtggtg gaatttttct cattctactg cccacactgt tatgattttg
     6001 aattaagcta caaaatccca agccaaatca aagaaaaatt accaagcgat agcaaattag
     6061 tgcaatatca cgttaatttc ttaggtcgcc aatctgaaga tttaactcgt gcttgggcat
     6121 tagcaatggc attaggtgcg gaagataaag taaaaaccgc attatttgaa ggtgctcaaa
     6181 aagacgcatt caaatcaatg gacgatattc gctctgtatt cttagcaaac ggcattacag
     6241 ctgaacagtt tgatagcggt attaatagct ttgcggtaaa tggcttagtg aataaacaag
     6301 ttcaattagc ggaagacttc caaattcgtg gtgtgcctgc attcttcgta aatggtcaat
     6361 accaactcaa cttagaaggc tttgcagatt cttcatcaac gaatgatttc atcaaacgtt
     6421 atgttgatgc ggttgtgttc ttaagcaaga aataacccct taaaatagtt agggttttta
     6481 caatgctgag taggtaaatc ctattcagca ttttttatat gcaaactcgg agaacaaaat
     6541 gacaaagtta tctatcctaa accttgtacc tattcgtgaa gggcaaactg ccaaacaagc
     6601 ggtcgattct atggtgaaat tagcaaaaca cgcggaaagt atcggcattg aacgctattg
     6661 gattgcagag caccacaata tgaaaaacct tgccagctcg gcaacacagc tcctcattca
     6721 gcatgccctt tctcacactg aaaaattaag agtgggatca ggtggcgtga tgttgcctaa
     6781 ccatagtcct tatattgtgg cagagcaata cggtacgctt gaaacactct accctaaccg
     6841 tgtagaactt gggcttgggc gagcaccagg caccgatatg cgaaccgcta ccgctttacg
     6901 ccgtaatgca caaagtctgc catttcctga tgaaatcagc gaactaaaag gttattttga
     6961 aggcacaaat tctgtgtctg cctaccctgc acaggggttg aatgtgccgt tttatatttt
     7021 aggctcaagc cctgaaagtg cctatttagc agcagaacgt ggcttgcctt atgcctttgc
     7081 ttcacatttc gctcctcgtt ttatggacga tgcggtgcga atttatcgca cacactttaa
     7141 gccgtctgaa gtgttagata aaccctatgt gattttaggt gtgaatgcga ttgcggcaga
     7201 aaccgaccaa gaggcagaac agcttgctac cactcagact cagtttttct taaatgttgt
     7261 taccaatgcc caaaactatt tacaaccgcc cgtcgaatcc gatgataaag tgtgaaaaaa
     7321 ttttgtgaaa gcggaaatcg taccgcattt cgggcctgtt tcgttccaac aaaatgcgtt
     7381 atacaaccaa gagaaacccg ttgttcgcca aatgactgct tgttcactta tcggtagccg
     7441 agagagcatt gaacaacagc tcaagtcatt aaaagcacgc attgaaattg atgaaattat
     7501 ggcggtaagc tacattttcg atgagcaaaa acagcatcaa tcctatactt ggttaaaaga
     7561 gattgcagat aaggtgtgat atgagcaaaa caatgcaaca aaaagccgct gatgcacata
     7621 atatgtgtaa gctaaaaggc gactctatgt tagataactc agaccgccaa attgcctttg
     7681 aagcagtgta tgatagtgaa gaaatagctg tacaagcggt cgaatttttc acacaaaaag
     7741 caaaatcggt agaaaccgag ccttgcgaca ttcaaagtga aatcacaccc attatagatg
     7801 gctttctgat gaaaatgtgg ataactttca gttgccaagc agaagtaatc ttatttcaaa
     7861 tggcggtgag atagatataa a
//
LOCUS       DANLXF010000108         7843 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.108, whole genome shotgun sequence.
ACCESSION   DANLXF010000108 DANLXF010000000
VERSION     DANLXF010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7843
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.108"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            42..977
                     /gene="pheS"
                     /locus_tag="PXD66_001528"
     CDS             42..977
                     /gene="pheS"
                     /locus_tag="PXD66_001528"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDL6319819.1"
                     /translation="MDQNLEALEEFRVEYFGKKGHFTTLMQELRNIAAEERPAIGAKI
                     NEAKQVVLDVLNAKKEEWEQAVLNAKLANESIDVSLPGRKTELGGLHPVSITIERVVK
                     FFSELGFTVANGPEIETDYYNFDALNIPDHHPARADHDTFWFDANRLLRTQTSGVQIR
                     TMENVKPPIRIVAPGRVYRNDYDQTHTPMFHQIELLYVDKEANFTELKGLIHDFLKAF
                     FEEDLQVRFRPSFFPFTEPSAEVDVMRQNGKWLEVLGCGMVHPNVLRNVGIDPEEYSG
                     FAVGMGVERLTMLRYNVTDLRSFFENDLRFLKQFK"
     gene            1025..3412
                     /gene="pheT"
                     /locus_tag="PXD66_001529"
     CDS             1025..3412
                     /gene="pheT"
                     /locus_tag="PXD66_001529"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDL6319820.1"
                     /translation="MKFNESWLREWVNPAISTEQLCDQITMLGLEVDDVEPVAGAFSG
                     VVVGEVVECAQHPDADKLRVTKVNVGGERLLDIVCGAPNCRQGLKVACAVEGAVLPGD
                     FKIKKTKLRGQPSEGMLCSYSELGIKEDHSGIIELPADAPIGQDFREYLNLNDVAIEI
                     SLTPNRADCLSIAGIAREVGVINRVDVKAPTITDVKATISDKVSVELQAPEACPRYLA
                     RVVKNVNVKATSPLWLQEKLRRCGIRSIDPIVDITNLSLLELGQPMHAFDAAKIEGGI
                     QVRMAKEGEELVLLDGTNAKLQSNTLVIADSKGALAMAGIFGGEASGVSENTKDVVLE
                     SAFFAPLAITGRARQYGLHTDASHRFERGVDPELTRQAMERATALLLEICGGEAGEIV
                     EAVSQENLPKRNTVTLRRSKLDSVIGHHIDDATVTDILTRLGLNVTFADNSWTAIAPS
                     WRFDIEIEEDLIEEIARIYGYNSIPNNSPLAHLTMKGTPEKLLEANRIRMALVDSDYQ
                     EVITYSFVDPKKQSLLHPQQEALILPNPISSEMSAMRVSLLTGLLDTIVYNQSRQQNR
                     VRIFEAGLRFIPDSNAESGVRQEFVFGAAIVGDKRPVHWENKSEAADFFDLKGDIERI
                     LSLTSIRHDLQFVAKQFPALHPGQSAAIMLDGKEIGFIGTVHPSIVQKFGIKGKPIVC
                     EILASAIAERPVTQAGEISKFPANNRDIAVVVDESVPAGEVLEACRKAGGNKLVAVNL
                     FDVYRGSNLESGKKSLAISLTVQDNEKTLEENEINAVIQAVLAELATRFNAVLRD"
     gene            3470..3766
                     /locus_tag="PXD66_001530"
     CDS             3470..3766
                     /locus_tag="PXD66_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005710633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HDL6319821.1"
                     /translation="MALTKIEIAENLIEKFGLEKRVAKQFVELFFEEIRSSLENGEEV
                     KLSGFGNFSLREKKARPGRNPKTGENVAVSARRVVVFKAGQKLRERVEQASSQS"
     gene            3805..4326
                     /locus_tag="PXD66_001531"
     CDS             3805..4326
                     /locus_tag="PXD66_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL6319822.1"
                     /translation="MKLTKRWLSLGCLLAITAMLVACSSSDTTSKSQVKSKVYRSIYQ
                     SDLSDPIMAISRLSEHQHEWKGTRYRLGGNSKAGIDCSGFMQVTFKDLFGIDLPRTTT
                     EQAKAGERISKDELRTGDLVFFKTGRGPNGKHVGVYVKNGQFLHASTKGGVIYSDLDS
                     PYWSKAFWQARRL"
     gene            4342..5385
                     /gene="degS"
                     /locus_tag="PXD66_001532"
     CDS             4342..5385
                     /gene="degS"
                     /locus_tag="PXD66_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HDL6319823.1"
                     /translation="MLKKIGQAVIFGLFCAILILFVAPIIQSKGSSFSLSAFTQKEIV
                     SYNAAVKIASPAVVNVYSRNFSSENETFEVKNLGSGVIMSSDGYILTNRHVIEDADQI
                     VIALQNGAISTNVKLVGDDSLTDLAVLKIEGENLPTIPQNPERKLQIGDVVLAIGNPL
                     NLGQSITQGIVSAIGRKTLTESGRQNFIQTDVSINQGNSGGALINTAGELIGINTLRF
                     GKNTNELSEGLNFAIPINLANQIMSKIIKDGRVIRGYFGVSTDLLDTAKMQGYEKGVI
                     IVNVDKNGPAAKAGIQAGDLVLKVGDIEAFSPSQMMEELAEMKPYTTVKVLVQRDEQL
                     LNFDVTITELQTQ"
     gene            5394..6068
                     /gene="cmk"
                     /locus_tag="PXD66_001533"
     CDS             5394..6068
                     /gene="cmk"
                     /locus_tag="PXD66_001533"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDL6319824.1"
                     /translation="MKKIVITVDGPSGAGKGTLCHALAEKLGFDFLDSGALYRITALS
                     AIKKEVPLDDEEKLAEVGRNLDIQFLPENGEIQVILEGENVGDLIRTAEAGQNASKVA
                     AFPLVREALLERQRAFSSEKGLIADGRDMGTVVFPDAQIKLFLDASAEERTKRRVKQL
                     QEKGFNANFDEILAEIKERDFRDRNRSVAPLVPAKDALLLDSTHLSIEDVINQALNHI
                     AQYIKF"
     gene            6160..7827
                     /gene="rpsA"
                     /locus_tag="PXD66_001534"
     CDS             6160..7827
                     /gene="rpsA"
                     /locus_tag="PXD66_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDL6319825.1"
                     /translation="MSELSFAQLLEESFAATPRLGDVVKGTVVAIQKGLVIVDTGFKS
                     ESAIDASEFTNAQGELEVQVGDEVDVVLKAVEDGFGATVVSRGDAKRNEAWIALEKAF
                     EEQATVIGFVNGKVKGGFTVELNGVRAFLPGSLVDTRPVRDSLNLEGKEYEFKVIKLD
                     QKRNNVVVSRRAVIETESNQDRDAVLANLVEGSEVKGTVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEVVNVGDEVTVKVLKFDKDKTRVSLGLKQLGQDPWAVIAENHPV
                     NSKLTGKVTNLTDYGCFVEILDGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLE
                     VDEERRRISLGLKQCKANPWEQFAATHSKNDKVSGKIKSITDFGIFIGLEGGIDGLVH
                     LSDISWNVSGEEAVRNYKKGDEVEAVVLQVDAVKERISLGIKQLESDPFTNFVDGTKK
                     GAILTGKVVEVDAKGVKVELEGGVEAFIRANEATAERVEDITSVISVGDSIEAKYTGV
                     DRKSRVVNLSVRAKNEAEESAAIAQVNKEEVVVPNAFAEAFKAAKGE"
BASE COUNT         2450 a         1283 c         1833 g         2277 t
ORIGIN      
        1 taaaagattt aactaataaa gcacgtgaag ctttagataa gattgatcaa aatttagagg
       61 ctttagaaga gtttagagtc gaatattttg gtaaaaaagg gcattttaca acgttaatgc
      121 aagaattacg caatattgca gcggaagaaa gaccggcaat cggagcaaaa attaatgaag
      181 caaagcaagt tgtgcttgat gtgttaaatg ccaaaaaaga agaatgggaa caagctgtat
      241 taaatgcaaa attagctaat gagagcattg atgtttcact gccggggcgt aaaactgaat
      301 taggtggttt gcaccctgtt tctatcacta ttgagcgtgt ggtgaaattt ttctctgaat
      361 taggttttac cgtagcaaac ggacctgaaa ttgaaaccga ttactataac ttcgatgcgt
      421 taaacattcc ggatcatcat ccggctcgag cggatcacga tacgttttgg tttgatgcca
      481 atcgtttatt aagaacccaa acctcgggtg tgcaaatccg tacaatggaa aatgtaaaac
      541 cgcctatccg tattgtggca ccggggcgtg tatatcgtaa tgactacgac caaacccaca
      601 caccgatgtt tcaccaaatc gaattgttat atgtggataa agaagcaaac tttaccgagt
      661 taaaaggctt aattcacgat tttctcaaag cattctttga agaagattta caagtgcgtt
      721 tccgcccgtc attcttccca ttcactgagc cttctgcaga agtagatgta atgcgtcaaa
      781 acggcaaatg gttagaagtg ttaggttgcg gtatggtaca cccgaatgtg ttacgcaatg
      841 tagggattga ccctgaagaa tatagcggtt ttgctgtggg aatgggcgtc gagcgtttaa
      901 cgatgttacg ctacaatgtc acggatttac gttcattctt tgaaaatgac ttacgtttct
      961 taaaacaatt taagtaatac aagcggttaa aaatttagaa aattttacaa ataggataga
     1021 aataatgaaa ttcaatgaat cttggttgcg tgaatgggta aacccagcaa ttagcacaga
     1081 acagttatgc gaccaaatta caatgttagg gttagaagtt gacgatgtag agccggtagc
     1141 tggtgcgttt tcgggtgtag ttgtgggtga agtggtggaa tgcgcccagc acccggatgc
     1201 tgacaaattg cgtgtcacta aagtgaatgt tggtggcgaa cgtttattag atattgtttg
     1261 tggtgcacca aactgccgtc aaggtttaaa agtggcttgc gctgttgaag gtgcagtgct
     1321 accgggcgat ttcaaaatta agaaaaccaa attgcgtggt cagccttcag aggggatgtt
     1381 atgttcttac tcagaacttg ggattaaaga agatcatagc ggtattattg aattacctgc
     1441 tgatgcacct attggtcaag atttccgtga atatttaaat ttaaatgatg tggcgattga
     1501 aatcagcctt acgccaaacc gtgccgactg cttaagcatt gccggcattg ctcgtgaagt
     1561 gggggtgatt aaccgtgtcg atgtgaaagc tccaactatt acagatgtaa aagccacgat
     1621 ttcagataaa gtttcagtgg aattacaagc accggaggct tgccctcgct atttagcccg
     1681 tgtggtgaaa aatgtgaatg tcaaagcgac atcgccactt tggttacaag aaaaattacg
     1741 ccgttgtggg attcgctcaa tcgatccaat cgttgatatt actaacttga gccttttaga
     1801 attagggcag ccaatgcacg cctttgatgc agcgaaaatt gaaggtggca ttcaagtgcg
     1861 tatggcaaaa gaaggcgaag aattggtatt gctagatggc acaaacgcta aattacaatc
     1921 gaatacctta gtgattgctg atagcaaagg ggcattggct atggcaggta ttttcggtgg
     1981 cgaagccagc ggggtgagcg agaatacgaa agatgtggtg ttagagtctg cattttttgc
     2041 tccattagcg attaccggtc gcgctcgtca atatgggtta cataccgacg catcacaccg
     2101 ttttgagcgt ggggttgatc cagaattaac tcgtcaagca atggagcgtg caacggcatt
     2161 attactggaa atttgcggcg gtgaagcagg tgaaattgtg gaagccgtga gccaagaaaa
     2221 cctaccaaaa cgcaatactg tcacgcttag acgcagtaaa ttagattcgg tgattggtca
     2281 tcatattgac gatgcaacgg taaccgatat tttaacccgc ttaggcttga acgtaacatt
     2341 tgcggataat agttggacgg caattgctcc aagttggcgt tttgatattg aaattgaaga
     2401 agatttaatt gaagaaatcg cgcgaattta tggctacaac agtatcccaa ataattcgcc
     2461 acttgctcat ttaacgatga aaggtacgcc ggaaaaatta ctggaagcaa accgtattcg
     2521 tatggcattg gtagatagtg attaccaaga agtgattact tatagctttg ttgatccgaa
     2581 aaaacaatca ttattgcacc cacagcaaga agccttgatt ttaccaaacc cgatttcaag
     2641 tgaaatgtcg gcaatgcgtg tttctctttt aaccggtttg ttggacacta tcgtgtataa
     2701 ccaaagccgt cagcaaaacc gtgtgcgtat ttttgaagca ggtctacgtt ttattccgga
     2761 tagcaatgcg gaatcaggcg tgcgtcaaga atttgttttt ggtgcggcga ttgtaggcga
     2821 taaacgtccg gtacattggg aaaacaaatc agaagcagcc gatttctttg atttaaaagg
     2881 cgatattgag cgtattcttt ctttaacctc tatccgccac gatctgcaat ttgtcgcaaa
     2941 acagttccca gcattacacc cggggcaatc ggctgcgatt atgcttgatg gcaaagaaat
     3001 tggttttatc ggaactgttc acccgtcaat tgtgcaaaag tttggcatta aaggcaaacc
     3061 gattgtatgt gaaattctag catcagctat tgcagagcgt ccggttacac aagcgggcga
     3121 aatttctaaa tttcctgcaa ataaccgtga tattgcggta gtggttgatg aatctgttcc
     3181 tgcaggtgag gtgttagagg cttgtcgtaa agctggtggt aataaattag tggcagtaaa
     3241 tttatttgac gtttaccgtg gttcgaattt agaaagtggt aagaagagtt tagccattag
     3301 cttaacggtt caagataatg aaaaaacatt agaagaaaat gaaatcaatg cggtaattca
     3361 agcggtatta gctgaattag caacacgttt caatgcggta cttcgtgatt aatcagtaaa
     3421 atatggctga tttattttat cagccataac aataaactaa ggataaatga tggcgcttac
     3481 caagattgaa attgcagaaa acctgattga aaaatttggg ttagaaaagc gtgtagcgaa
     3541 gcaatttgtt gaattgttct ttgaagagat ccgatcctcc ctcgaaaatg gtgaagaagt
     3601 gaaattatct ggttttggta atttttcact gagagaaaaa aaagctcgtc caggaagaaa
     3661 tcctaaaacg ggggaaaatg tggctgtttc agcccgtcga gtagtagttt ttaaagctgg
     3721 gcaaaaatta cgagaacgtg ttgagcaagc tagctcccaa tcataggggc agttatatta
     3781 gttagattta atgtggagta ataaatgaaa ttaaccaaac ggtggctttc cttaggttgt
     3841 ttgttagcta ttacagcgat gttagtggct tgttcaagtt cagatacaac ctctaagtct
     3901 caagtaaaaa gtaaagttta tcgttcaata taccaatcag acctttctga tcctattatg
     3961 gcaattagtc gattaagtga acatcaacac gaatggaaag gcactcgcta tcgtttaggt
     4021 ggcaatagca aggctggcat cgactgttct ggttttatgc aagtcacttt taaagatctt
     4081 tttgggatag atttgccgag aaccaccaca gagcaggcta aggctggtga gcgtatatcc
     4141 aaagacgaat tgcgtacagg tgatttagtt tttttcaaga cagggcgtgg tccaaatggg
     4201 aaacacgttg gtgtttatgt aaaaaatggg caatttcttc acgcctcaac aaagggaggg
     4261 gtgatttatt ctgatttgga ttcgccatat tggtctaagg ctttctggca ggctcgtcgt
     4321 ttataattta gggtaacata catgctgaaa aaaatcggac aagcggtcat atttggactt
     4381 ttttgtgcaa ttttaatttt gtttgtagct ccaattattc agagtaaagg gagttcgttt
     4441 tcattatcag cattcactca aaaagagatt gtaagctaca atgcagcggt taaaatagcc
     4501 tcccccgcag tcgttaacgt atatagccga aattttagtt ctgaaaatga aacatttgaa
     4561 gttaagaatt taggttcggg tgtgattatg agtagcgatg gctatatttt aactaacagg
     4621 catgtgattg aagatgcgga tcaaatcgtg attgctttgc aaaacggtgc tatttcgact
     4681 aatgtaaaat tagtcggcga tgattcttta actgatttag ctgttttaaa aattgagggt
     4741 gagaatttac ctactattcc acaaaatccg gagcgcaaac tacaaattgg cgatgtggtg
     4801 ttggcaattg gtaatccttt gaatttagga cagagcatta cgcaagggat agtcagtgct
     4861 attgggcgaa aaaccttaac agaaagtggt agacaaaatt ttattcagac agatgtttcc
     4921 attaatcaag ggaactcagg tggtgcttta attaatactg ctggtgagct aataggtatt
     4981 aacactttgc gttttgggaa aaatacgaat gaattgtcag agggcctaaa ttttgcgatt
     5041 cccattaatc tggcgaatca aattatgagt aagatcatta aagatggccg agttattcgt
     5101 ggctattttg gggtaagtac agatttgctt gacaccgcaa aaatgcaagg ttatgaaaaa
     5161 ggggtgatca ttgttaatgt agataaaaat gggccagccg caaaagcggg gattcaagca
     5221 ggtgacttag tgcttaaagt cggagatatt gaggcattct ctccttctca aatgatggaa
     5281 gagcttgctg aaatgaaacc ttatactaca gtaaaagtat tggttcaacg tgatgaacaa
     5341 ttacttaact ttgatgtaac tattactgag ttacagactc aatagggtag aaaatgaaaa
     5401 aaatagtgat tacagttgat gggccgagtg gtgcaggtaa aggaacgtta tgtcatgcat
     5461 tggctgaaaa attagggttt gattttttag attctggagc cttataccgg attactgcat
     5521 taagcgcaat taaaaaagag gttccccttg atgatgagga aaaactagcg gaagttgggc
     5581 gtaatcttga tattcaattt ttgccagaaa atggcgagat tcaggtgatt ttggaaggag
     5641 aaaatgtagg cgacttaatc cgaacggcag aagcaggtca aaatgcctct aaagtcgctg
     5701 ctttcccttt ggtacgtgaa gcacttttag aacgccaaag agcctttagt agcgaaaaag
     5761 gtctgattgc agatggacga gatatgggga cagttgtgtt tcctgatgca caaattaagc
     5821 tgtttttaga tgcaagtgcc gaagaacgca caaaaagacg tgtaaaacag ttgcaagaaa
     5881 agggatttaa tgctaacttt gacgagattt tagccgagat aaaagaacgt gattttcgcg
     5941 atagaaatcg atcagtcgcg ccacttgttc cggctaaaga tgcgttgctg ttagattcaa
     6001 cgcatttatc aattgaagat gtgattaacc aagcattaaa tcatatcgct caatacatta
     6061 aattttagtt tgttgcttca cataggattt gtgtagcttt tattatcaac cccgtttaac
     6121 aggacgttag atggatgttt atttttaaga agatttatta tgtcagaatt atcgtttgct
     6181 caactacttg aagaatcatt tgcagctaca ccacgtttag gtgatgttgt taaaggtact
     6241 gttgttgcta tccaaaaagg cttagttatt gttgatacag gtttcaaatc tgaatctgca
     6301 atcgatgcct cagaatttac taacgcacaa ggtgaattag aagttcaagt gggtgatgaa
     6361 gtagatgttg ttcttaaagc tgtagaagac ggttttggtg caacagtagt ttctcgtggt
     6421 gatgcaaaac gtaacgaagc ttggattgct ttagagaaag catttgaaga acaagctaca
     6481 gttatcggtt tcgtaaatgg taaagtaaaa ggcggtttca ctgttgaatt aaacggtgtt
     6541 cgtgcattct tacctggttc attagttgat actcgtccgg ttcgtgattc tttaaaccta
     6601 gaaggtaaag agtacgaatt caaagtaatc aaattagatc aaaaacgtaa caacgtagta
     6661 gtatctcgtc gtgctgttat cgaaacagaa agcaaccaag atcgtgatgc ggtattagca
     6721 aacttagttg aaggttctga agttaaaggt acagttaaaa acttaactga ctacggtgca
     6781 ttcgtagatt taggtggcgt tgacggttta ttacatatca cagatatggc ttggaaacgt
     6841 gttaaacacc caagtgaagt ggttaatgta ggcgatgaag ttactgttaa agtattaaaa
     6901 tttgacaaag acaaaactcg tgtatcatta ggcttaaaac aattaggtca agatccttgg
     6961 gctgtaattg ctgaaaacca cccggtaaac agcaaattaa ctggtaaagt aactaactta
     7021 actgactacg gttgcttcgt tgagatttta gacggtgttg aaggtttagt tcacgtttca
     7081 gaaatggatt ggacaaacaa aaacattcac ccatctaaag tggttaacgt aggcgatgta
     7141 gttgaagtaa tggtacttga agttgatgaa gaacgtcgtc gtatctcatt aggtttaaaa
     7201 caatgcaaag caaacccttg ggaacaattt gcggcaactc acagcaaaaa tgataaagta
     7261 tcaggtaaaa tcaaatcaat tactgacttc ggtattttca tcggtttaga aggtggtatt
     7321 gacggtttag ttcacttatc tgacatttct tggaatgttt caggtgaaga agcagttcgt
     7381 aactacaaaa aaggtgatga agtagaagct gttgttctac aagttgatgc agtgaaagag
     7441 cgtatctctt taggtatcaa acaattagaa tctgatcctt tcacaaactt tgtagatggt
     7501 actaaaaaag gtgcaatctt aacaggtaaa gttgtagaag ttgatgctaa aggcgttaaa
     7561 gttgaattgg aaggtggcgt tgaagcattt attcgtgcaa acgaagcaac tgctgagcgt
     7621 gtagaagaca tcacttctgt tatttctgtt ggcgattcaa tcgaagcgaa atacaccggt
     7681 gttgatcgta aatctcgcgt agttaactta tctgttcgtg caaaaaacga agctgaagaa
     7741 tcagcagcaa ttgcacaagt gaacaaagag gaagttgttg tacctaatgc atttgctgaa
     7801 gctttcaaag cagctaaagg cgaataatta gtatagacta atc
//
LOCUS       DANLXF010000109         7625 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.109, whole genome shotgun sequence.
ACCESSION   DANLXF010000109 DANLXF010000000
VERSION     DANLXF010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7625
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.109"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            197..2653
                     /gene="thrA"
                     /locus_tag="PXD66_001535"
     CDS             197..2653
                     /gene="thrA"
                     /locus_tag="PXD66_001535"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HDL6319826.1"
                     /translation="MRVLKFGGTSLANPERFMQAAEIIEKAHLADQAAGVLSAPAKIT
                     NHLVAIVDKAIAGESFQSNLSEATEIFHNIINGLYATNNHFAKEALLALVEAELNQIQ
                     QVAAEAAQTKSLSDKIAATIHCRGEKLSIAMMQAWFEAKGYEVTRIDPVEKLLAHGSY
                     LESSVDIAESTKRVDAGSIPKKNIVLMAGFTAGNEQGELVLLGRNGSDYSAACLAACL
                     KADVCEIWTDVDGVYTCDPRLVPDAVCLETMSYQEAMELSYFGAKVIHPRTIGPLVPL
                     NIPCLIKNTGNPDGKGTLIDGNVANDNLKVKGITNLDNVAMFNVSGSGMQGMVGMAAR
                     VFSTMSKAGISVILITQSSSEYSISFCVPAKSAEKALAALNTEFAQELSENLLDPIDI
                     IKDLSIVSVVGDGMRTAKGIAARFFSALAQANISIVAIAQGSSERSISAVVPLNKAIE
                     AVKATHKSLFNSKRVVDMFLVGVGGVGSELIEQVKQQKEFLAKKDIEIRVCALANSDK
                     MLLNENGLNLDNWKEDLEKAIQPSDFDVLLSFIKFHHVVNPVFVDCTSAQSVANLYAR
                     ALKEGFHVVTPNKKANTSGYDYYLEMRENARQSQHKFLYETNVGAGLPVIENLQNLLA
                     AGDEVEKFEGILSGSLSFIFGKLEEGLTLSQATLIAKEKGFTEPDPRDDLSGADVARK
                     LLILVRETGLPLEFEDIKIEGVLPKGFSEGMSRDEFLQALPSLDEEFSKRVEAAKAEG
                     KVLRYVGSITGDKCRVAIEAVDENHPLYKVKDGENALAFLTRYYNPIPLLLRGYGAGN
                     SVTAAGIFADILRTLQGGVN"
     gene            2653..3600
                     /gene="thrB"
                     /locus_tag="PXD66_001536"
     CDS             2653..3600
                     /gene="thrB"
                     /locus_tag="PXD66_001536"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDL6319827.1"
                     /translation="MALRIYAPASSANISVGFDSLGAAISPIDGSLLGDVVQIEESEQ
                     QFELESAGYFVRKLPKEPQKNIVYQAYVLFSERLKLRNGSVKNLRLTLEKNMPIGSGL
                     GSSACSIVAALVALNKFHDEPFSKMELLEMMGELEGRISGSIHYDNVAPCYLGGLQLM
                     TQSLGNICQPLPFFDEWYWVLAYPGVEVSTAEARAILPKSYTRQDMIQQARYLGSFVH
                     ACHTHQDILAATMMKDLIAEPYREDLLPNFPAVRQGCKDLGALAVGISGSGPTMFAIA
                     PDLEHAQRLVSYLEKEYLQNNEGFIHICKVDNQGARELK"
     gene            complement(3715..5091)
                     /gene="cysS"
                     /locus_tag="PXD66_001537"
     CDS             complement(3715..5091)
                     /gene="cysS"
                     /locus_tag="PXD66_001537"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDL6319828.1"
                     /translation="MLKIYNTLKREKEEFKPINPNQVGMYVCGVTVYDLCHFGHGRTF
                     VSFDVIARYLRYSGYNLRYVRNITDVDDKIIKRALENNETCDELVDRMIAEMHKDFDA
                     LNILRPDVEPRATKHIPEIIAMVEKLIHNGHAYVAADGDVMFDVESFQKYGALSRQNL
                     EQLQAGARVEIKSIKKNPMDFVLWKMSKAGEPSWESPWGNGRPGWHIECSAMNSKELG
                     EHFDIHGGGSDLMFPHHENEIAQSCCAHGGDYVNYWLHTGMLTIDKEKMSKSLGNFFS
                     IRHMLGLYDAESLRYFFLTAHYRSLLDYSIDNLDLARSALERLYTALRGCDVSGEAIG
                     EHYVEAFKTAMDDDFNTPGALAVLFEIARELNKLKTEDMAKANGLAVRLKELAGVLGL
                     LEQDPDAFLKGDANDDETAEIEALIKQRNEAKAAKNWAVADEVRDKLKAINIVLEDTP
                     NGTIWRKA"
     gene            5247..5756
                     /locus_tag="PXD66_001538"
     CDS             5247..5756
                     /locus_tag="PXD66_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL6319829.1"
                     /translation="MITLHTNFGDIKIELNFEKAPITAKNFEDYAKEGFYNGTIFHRV
                     IDGFMIQGGGMTAGMAEKPTKDPIQNEANNGLSNKRGTIAMARTSDPHSASSQFFINV
                     ADNTFLDYRSKEMFGRSVVQDWGYAVFGEVVEGMDVVDKIKGVKTGNKGFHQDVPVED
                     VVIESVSVA"
     gene            complement(5848..6777)
                     /locus_tag="PXD66_001539"
     CDS             complement(5848..6777)
                     /locus_tag="PXD66_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDL6319830.1"
                     /translation="MEIALLLADKILQLTLMVLLGYLIVKTKLLKSEHSYPISVIGLY
                     IISPAMLITAFQVDYTTEILNGLYLAFLISVLLNGLLILLGWLLKRALKLDNIEQATA
                     IYSNSGNLIIPIVASLFGNEWVIYSTAFIVVQNVLFWSHLRLLICGKGNVPFRKMIFN
                     INIIAIVIGMMLFLLHIKLPATLANTLSTLGHMLGPLAMLVAGMLIAAIPFKEIITDK
                     RIYLVAFLRLIFIPLILLAVIKLGGFASWIENGKTVVMITFLATISPSAATATQMAVA
                     YGQDAKKASAIYGVTTLLCVFTMPLIIWLYQFL"
     gene            complement(6780..7574)
                     /gene="fdhD"
                     /locus_tag="PXD66_001540"
     CDS             complement(6780..7574)
                     /gene="fdhD"
                     /locus_tag="PXD66_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDL6319831.1"
                     /translation="MNAKKLSLSTNSIKQKDDEENLISEVPVALVYNGISHTVMMATP
                     ANLEDFALGFSLAEGILNAPSELYGLDVIEQENGVEIQLEISSRQFVALKDKRRSMAG
                     RTGCGICGVEQLEQVNQSCKIVKKNNRLQNVNPLILEDCLLQLENAQFLSKETGASHA
                     AAFFTPDGKLLAIREDVGRHVALDKLIGWYAKADFPIGFVFVTSRASFEMVQKCLAVG
                     IEMLCAISATTEMAVNMARENQFTLLAFARKGKATIYAGEERLALN"
BASE COUNT         2241 a         1626 c         1603 g         2155 t
ORIGIN      
        1 tgaaccatta ttcactctct tgaccgagat tccgccaatg aattgtttta atatgatgac
       61 aacgattatt accaccatta tgtcaccaac atagtgcggg ttttaattgg ctgaaataat
      121 gaaagaataa accgaaaacc cgctacaagc gggttttttt gtataaaaat ttacaaataa
      181 tttatcagga gtaactatgc gagttcttaa atttggaggc acttctcttg ccaaccctga
      241 acgctttatg caagcggcag agattatcga gaaagctcat ttggcagatc aagctgccgg
      301 cgtattatcc gccccagcca aaattaccaa tcatcttgtc gctattgtag ataaagcgat
      361 tgccggtgaa tcatttcaat ctaacctttc tgaagctacc gagattttcc acaacatcat
      421 taacggctta tatgctacaa ataatcactt tgctaaagaa gcactgttag ccttagtgga
      481 agcggaatta aaccaaattc agcaagtggc tgcagaagca gctcaaacta aatcgttgtc
      541 agataaaatt gctgccacta ttcactgccg tggtgaaaaa ctctctattg cgatgatgca
      601 agcgtggttc gaggcaaaag gctatgaagt cactcgtatc gaccctgttg aaaaattatt
      661 ggctcacggc agctatttgg aatcttcggt cgatattgcc gaatccacca agcgagttga
      721 tgcaggttca attccaaaga aaaatatcgt gctaatggct ggttttaccg caggtaatga
      781 gcaaggcgaa ctggtgttat tagggcgtaa cggttcagat tattctgccg cttgtttggc
      841 agcgtgttta aaagccgatg tttgtgaaat ctggacggac gtagatggcg tttacacctg
      901 cgaccctcgt ttggtgcctg atgcagtatg cttagaaaca atgagctatc aagaggcaat
      961 ggagctttcc tactttgggg cgaaagtgat ccaccctcgc accatcgggc cattagtgcc
     1021 gctcaatatt ccttgtttaa tcaaaaatac cggcaatccg gacggaaaag gcacattaat
     1081 tgatggcaat gtggcaaacg ataatctcaa agtaaaaggg attaccaacc ttgataacgt
     1141 ggcgatgttc aacgtttcag gttcaggaat gcagggaatg gtgggcatgg ctgcccgtgt
     1201 gttctcgacg atgtcaaaag ccggtatttc ggtgatttta attacccaat catcatcaga
     1261 atatagcatc agcttctgcg tgccggcaaa atcggcagaa aaagcgttag cggctctaaa
     1321 tactgaattt gcacaagagt tatctgaaaa cttacttgac ccgattgata ttattaaaga
     1381 tttatccatt gtttcggtag tgggtgatgg tatgcgtacc gccaaaggaa ttgcggctcg
     1441 cttcttctct gccctagccc aagccaacat cagtattgtg gcaattgcac agggttcatc
     1501 tgagcgttcc atttctgctg tcgtgccgtt aaacaaagcg attgaagcag taaaagcaac
     1561 tcacaaatcg ctatttaaca gtaaacgtgt ggtggatatg tttttagtgg gtgtaggcgg
     1621 tgtgggtagc gaactcatcg agcaagtcaa acaacaaaag gagtttttgg caaagaagga
     1681 tattgagatc cgtgtgtgtg ctttagctaa ttcagataaa atgttactca acgaaaacgg
     1741 cttaaattta gacaactgga aagaagattt agaaaaagca attcagccgt cagactttga
     1801 tgtattgctt tctttcatca aattccacca cgtggtaaac ccagtatttg tagattgcac
     1861 ttcggcacaa tcggtggcga acctgtacgc acgagcctta aaagagggct tccacgtggt
     1921 aacaccaaac aaaaaagcca atacatcagg ttacgattac tacttagaaa tgcgtgaaaa
     1981 cgcccgccaa agccagcaca aattcctcta cgaaactaat gtaggggcag gtttaccggt
     2041 aattgaaaac ttacaaaacc tattagctgc cggtgacgaa gtagagaaat ttgaagggat
     2101 tttatccggc tcgctctctt tcattttcgg caaattagaa gaaggtttaa ccctttcgca
     2161 agcaacttta atcgccaaag aaaaaggctt taccgagcca gatccacgag atgatttatc
     2221 cggtgcagat gttgctcgta aactcttaat tcttgttcgt gaaaccggct taccgcttga
     2281 atttgaagat attaaaattg aaggcgtatt gccaaaaggc ttctcggaag gaatgagtcg
     2341 tgatgagttt ttacaagctc taccgtcttt agacgaagaa ttcagcaagc gtgtagaagc
     2401 tgccaaggct gaaggcaaag tgttgcgtta tgtgggctcg atcacaggtg ataaatgccg
     2461 agttgccatt gaagccgtgg acgaaaacca cccactttac aaagttaaag acggcgaaaa
     2521 tgcccttgct tttctcacac gttactacaa ccctattcca ttattattgc gtgggtatgg
     2581 tgcgggcaat agcgtaaccg ctgccggtat ctttgcagat attctgcgta ctttacaagg
     2641 aggagtgaac taatggcgtt acgaatttat gccccagctt caagtgcaaa tattagtgtg
     2701 ggttttgact cattaggcgc tgcaatctcc ccgattgacg gctctttgct cggcgatgta
     2761 gttcaaattg aagagagtga acaacaattt gagctagaaa gtgcaggtta ttttgtacgt
     2821 aaattgccca aagaaccaca aaaaaacatt gtctatcaag cctatgtgct atttagcgaa
     2881 cgcttgaaat tacgtaacgg cagtgtaaaa aatttacgct taacacttga aaaaaatatg
     2941 ccgattggct cagggctagg ctcaagtgcc tgctcgattg ttgcagcatt agtggcactc
     3001 aataaattcc acgatgaacc gttctccaaa atggaattac tggaaatgat gggcgaattg
     3061 gaaggcagaa tttcaggctc aatccactac gacaacgttg ccccttgcta tttaggtggc
     3121 ctgcaattaa tgacacaatc gctcggcaat atttgccaac ctctgccatt ctttgatgaa
     3181 tggtattggg tgcttgccta cccgggcgtg gaagtatcaa ccgccgaagc ccgtgctatt
     3241 ttgccgaaaa gctatactcg tcaagatatg attcagcaag cccgctactt aggatctttc
     3301 gtccatgctt gccatacaca tcaagatatc ttggctgcta caatgatgaa agacctaatt
     3361 gctgagcctt accgtgaaga tttactgccg aactttccgg ctgttcgtca aggctgtaaa
     3421 gacttaggtg cgttagcggt tggtatttca ggttccggtc cgacaatgtt tgcgatagcc
     3481 cctgatctcg aacacgctca acgtttagtg agctatttag agaaagaata tctgcaaaac
     3541 aacgaaggct ttattcatat ctgtaaagtc gataatcaag gagcaagaga gctgaaatag
     3601 ccctctcgaa caaccatatc actaaataac ttaaaggact gactccaata ccacttggag
     3661 cagccctttt tgattaattt tttgaaaagt ctatcactta taaacaatcg aaggctatgc
     3721 cttacgccaa attgtgccgt ttggggtgtc ttcgaggaca atatttatcg ctttgagctt
     3781 atctcgcact tcatcggcaa ccgcccagtt tttagccgct ttggcttcgt tgcgttgttt
     3841 gattaaggct tcgatttctg ccgtttcatc gtcattcgca tcacctttta aaaacgcatc
     3901 ggggtcttgt tccagtaagc ctagcacgcc ggctaactct tttaaacgaa cggctaaacc
     3961 gttggctttt gccatatctt cggttttgag tttgttcaac tcacgggcaa tttcaaataa
     4021 caccgctaat gcccccgggg tgttgaaatc atcgtccatt gcggttttaa aggcttctac
     4081 atagtgttct ccgatggctt cgcccgacac atcacaacca cgcagagcgg tatataaacg
     4141 ctcaagagcc gaacgggcta aatcaaggtt gtcaatgctg taatctaata acgaacggta
     4201 atgagcggtt aagaagaaat aacgtaacga ttcggcatcg tataagccta acatatggcg
     4261 aatgctgaag aaattgccca aagatttcga cattttttct ttatcaatgg taagcattcc
     4321 agtgtgtaac caataattga cataatcgcc accgtgagca cagcaagatt gtgcaatttc
     4381 gttttcgtga tgcgggaaca ttaaatccga gccaccgccg tgaatatcaa aatgttcgcc
     4441 caactctttg ctattcattg ccgaacattc aatatgccag cccggtctgc cattgcccca
     4501 cgggctttcc cagcttggct caccggcttt cgacattttc cacaagacaa aatccatcgg
     4561 gtttttcttg atggatttaa tttcaaccct tgcaccggct tgtagttgct ctaaattttg
     4621 gcgggataat gcaccatatt tctggaacga ttccacatca aacatcacat cgccatcggc
     4681 tgcgacataa gcgtgtccgt tgtgaatcag tttttctacc atcgcaataa tttcaggaat
     4741 atgcttggtg gctcgtggct caacgtccgg gcgaagaatg tttaacgcat caaaatcttt
     4801 gtgcatttca gcaatcattc tatccactaa ttcatcgcaa gtttcgttat tttctaacgc
     4861 acgtttgatg attttgtcgt ccacatcggt aatgttacgc acatagcgta aattgtagcc
     4921 tgaataacgt aaataacggg caatcacatc gaaagagaca aaagtgcgac cgtgcccaaa
     4981 atgacaaaga tcgtaaaccg tgacgccaca gacgtacatt cccacctgat ttgggttaat
     5041 cggtttaaat tcttcttttt cacgttttaa ggtgttataa attttgagca taaagtcctc
     5101 ttgtgtaaca ttcagtaaca aatggggcta agtatagcga ataagcggtc tgattttgag
     5161 atttttttgc aatttggcgt aaacttttta ataatagcga gcgaaattta ttttccattc
     5221 catttttatt tataaaggaa caaagaatga ttacattaca taccaatttc ggtgacatta
     5281 aaatcgaatt aaactttgaa aaagccccga ttaccgctaa aaactttgaa gattacgcta
     5341 aagaagggtt ttataacggc acgattttcc accgtgtgat tgacggcttt atgattcaag
     5401 gtggcggaat gacggcaggt atggctgaaa aaccgacaaa agatccgatt caaaacgaag
     5461 cgaacaacgg tttaagcaac aagcgtggca caatcgcaat ggctcgcact tctgatccgc
     5521 attctgcttc gtcacagttt ttcattaatg tggcggataa tactttctta gattatcgct
     5581 caaaagagat gttcggcaga agtgtagtgc aagattgggg ctatgcagta tttggcgaag
     5641 tggttgaagg tatggacgtg gtcgataaaa tcaaaggcgt aaaaaccggc aataaaggct
     5701 tccaccaaga cgtgccggtt gaagatgtgg tgattgagtc tgtaagcgtt gcctaattcc
     5761 gcaaattagc ctacaagcgg ttgatttttc acaatttttt gcatttctca gcgttgcaaa
     5821 aaagtagcag aaatcgactg cttgttttta tagaaattga tacagccaaa taatcagcgg
     5881 cattgtaaac acacaaagta aagtggttac gccataaatc gcactggctt ttttggcatc
     5941 ttgcccgtaa gccaccgcca tttgggtcgc cgttgcagca gaagggctga tggtggctaa
     6001 gaatgtaatc attaccaccg tttttccatt ctctatccag ctcgcaaaac cgccgagttt
     6061 aatcaccgct aacaaaatca gcggaataaa aatcaagcgg agaaaagcca ccaagtaaat
     6121 gcgtttatcg gtaataatct ctttaaatgg aattgctgca attaacatcc ctgccacaag
     6181 cattgctaaa ggccctaaca tatgccctaa agttgagagc gtgtttgcaa gcgtagcggg
     6241 cagtttgata tgtagcaaaa acaacatcat accaatcaca atcgcaataa tgttgatatt
     6301 aaagatcatc tttctaaacg gcacattgcc tttgccacaa atcagcagcc gtaagtgaga
     6361 ccaaaacaac acattttgca ccacaataaa agccgttgaa taaatcaccc attcattgcc
     6421 gaacagggag gcaacaatcg gaataattaa gttgccggag ttggaataaa tagcggtggc
     6481 ttgttctatg ttgtctaatt tcaacgctct tttgagcagc caaccaagca gaattaacaa
     6541 gccgtttaat aagacggaga ttaaaaatgc cagatacaag ccgtttagta tttcggtggt
     6601 gtaatctact tgaaaagcag taatcagcat tgccgggctg ataatgtaaa gtccgataac
     6661 cgaaatcgga taactgtgct ccgatttgag taatttagtt ttaacgatga ggtagccgag
     6721 cagcaccatc agggttaatt gtagaatttt atcggctaat aaaagggcta tttccattat
     6781 caattaagag cgagtctctc ttctcctgca taaatggtcg ctttgccttt gcgggcaaaa
     6841 gcaagtaagg taaattgatt ttcacgagcc atattcactg ccatttcagt ggtcgctgag
     6901 atggcacaca acatttcaat gccaacggct aagcatttct gcaccatttc aaagctggca
     6961 cgactggtta caaacacaaa tccaatcgga aaatcagctt tagcatacca accgattaat
     7021 ttatccaacg ccacgtgtct gcccacatct tcacgaatgg caagcagttt tccatccggg
     7081 gtaaaaaatg cagcggcgtg gcttgcaccg gtttctttcg ataagaattg agcattttct
     7141 agctgtaata aacaatcctc taaaatcagt ggattgacgt tctgcaagcg gttatttttc
     7201 ttaacaattt tgcaactttg attaacctgc tctaattgct ctacaccgca aataccgcaa
     7261 cctgttctgc ctgccattga gcggcgttta tcttttaatg caacaaattg gcggctcgat
     7321 atttcaagct gaatttctac tccattttct tgttcaatta catctaaacc atataattcc
     7381 gatggggcat ttaaaatccc ttccgccaat gagaatccta aagcgaagtc ctccaaatta
     7441 gccggtgttg ccatcattac agtatgagaa ataccgttat ataccaaagc aaccggcact
     7501 tctgaaatta aattttcttc atcatctttt tgtttaatac tattagttga taatgataat
     7561 tttttggcat ttatttttgt gatcttattc acaatattta tctctcactt gactaaaaag
     7621 tagtt
//
LOCUS       DANLXF010000110         7559 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.110, whole genome shotgun sequence.
ACCESSION   DANLXF010000110 DANLXF010000000
VERSION     DANLXF010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7559
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.110"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            250..918
                     /locus_tag="PXD66_001541"
     CDS             250..918
                     /locus_tag="PXD66_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL6319832.1"
                     /translation="MAHNGSGKSTLMRLLSGDITPTSGSILVDGLPLHQLSTKEAAKH
                     IAYLPQRLPEAADFLVQELVALGRFPYQKWLQKPTEFDVQIVEQAMESTSVTQFAKQP
                     VNSLSGGEKQRVWLAMCLAQQTPYLLLDEPLAALDIVYQVEVMQLIRKLVNEQGLTVI
                     IIIHDINLASQFCDEIIALKQGELCIQGSVDEIMQEQKLLEIFGLKLHLFNHPAGLHK
                     VAVL"
     gene            915..1796
                     /locus_tag="PXD66_001542"
     CDS             915..1796
                     /locus_tag="PXD66_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL6319833.1"
                     /translation="MMFRQYKRFIFLFILQISTAFAQPNIFTPDWSIASALTEMGNPP
                     IAMGDKRIYPLWSHRPIQPNSVLDIGARYQPNRELLAQLPVELVLSNFFYAHLASVYP
                     KEIPQIDVLFDGGNTEPQQHWHTYQKATRMIGTEIKNPNAAEAYLTKTEQTLAQWGKE
                     ILNSNPDIDYYSVVQFGSPRELRIYASNSMFRVAFDLMGLKQADLGVGDRWGNKLIPV
                     SDLAKLPERTCLIVISPFSKMTQADLENSYIWQRLGFGKNRCMKVLPPVWLFGGADSI
                     SHFAEFLHNAMTQGEKE"
     gene            1796..2611
                     /locus_tag="PXD66_001543"
     CDS             1796..2611
                     /locus_tag="PXD66_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL6319834.1"
                     /translation="MKSSFLLIGCILLSLVTAVIRLNMVWEWEWQLLLINVDLLPINQ
                     AAVKAALLPEMTTSFLAGGLLAIASSGLQHIVRNNLASDSTLAVSSGAQLALMIAVIF
                     FPAAELFSSFWIALLGSLAAIVLVLFIAKASKLNPLTLILGGLIVNILFGAIASLLTL
                     FYFDFLFGVMVWGSGSLLQDGWATSITLAITVVVAFFIFVLLSRPLTILSLDDEQARR
                     LGAPVNLLRYLVIFVCAAITALVVSKIGVIGFIGFAVQVWLILPKYDIYYFAS"
     gene            2608..3744
                     /locus_tag="PXD66_001544"
     CDS             2608..3744
                     /locus_tag="PXD66_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL6319835.1"
                     /translation="MIAFLAGGFLLFFTNNLIGIVSRIYDTLLPVGAFTATLGAPLLI
                     FLVLRQRKAQFEASNTAYFPVKRLNSWKLPILLLCAGVILFCLLQIIAPTMNGWAISI
                     DENLMSSYRLPRSLSAIATGAMLAVSGSLLQILTRNPMASPEVLGVSSGATIAAVLSF
                     LLFPTLSSISFLLFGVLGSLTVLALIMWLSRKLQPSALMITGVAIGALVSGLMAIIQL
                     SGSPRLTAVISWLSGSTYYANPNTVWILLSVAILLISATLLFIKPLRLISLGETVSYH
                     LGLEVKKVQMIVLLLIALMSAASTLAVGPMSFIGLMTPHLAKNLGAITPEKQIPISAL
                     LGAMLMLIADWLGRYLIFPYEIGAGIIASILGGIYFLLLLKQHK"
     gene            3772..4170
                     /locus_tag="PXD66_001545"
     CDS             3772..4170
                     /locus_tag="PXD66_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HDL6319836.1"
                     /translation="MNTLSKAIKLALYSSGIGFISTAYAETVTHLDTVEVSAETPNAS
                     SEETGRYAQKKAKGSNGMALSAKDTPQSVTAVTHQQIRDQNLNTIAKALEATHGVSVS
                     LLDRGRYSFSARGFGIDKVKVDGMDLKVSK"
     gene            4205..5908
                     /locus_tag="PXD66_001546"
     CDS             4205..5908
                     /locus_tag="PXD66_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HDL6319837.1"
                     /translation="MLYDRVEVVRGATGLTTGAGDPSASVNLVRKRANSISRYTILEA
                     GIGRYADFNTMIDHSQALNNSGSVRGRFIAEHKNGNTFIKNEKERYTTLYGNIEADLT
                     DTTKLGLGVSYQKEKRDAALWGGLPAFYSDGGETNWSRSKNASPEWAYWDNESVNYFA
                     DITQKLGEKWELALKGNYRVGKHNSELFYFSGSSLNRQDGLGWSPWPGKFKNDNTQSN
                     VQLDLTGSFSAWGLEHDAAIGIQYNRAHRNSYAADNSTFDGAFDFNNWNGIYARPNWG
                     SMSEKYYQNLKEFGVYVSTRLHLTDRLSMLLGSRISSYRTEGTFYGSTQNFHANDVWT
                     PYAGLTFDINPQNTVYVSYTDIFKPQSQVDINNKLLSPVTGETYEAGWKGSYFNDNLQ
                     TQISVFETRQDNLAQSAGINPNTLLAYYTASKGAKVRGFEVEASGNVTEKLKLSAGYT
                     QWKGDDASGNPINTTQPRKQFKLFATYDMNRLVQGLTVGAGVNWQSRIYTKHATYGEY
                     GQKAYTLVNLMARYQFNDNFSAQLNIDNLFDKKYRNALSFSQYSYGEPFYTRLNLRYE
                     F"
     gene            6020..6664
                     /gene="adk"
                     /locus_tag="PXD66_001547"
     CDS             6020..6664
                     /gene="adk"
                     /locus_tag="PXD66_001547"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005721186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDL6319838.1"
                     /translation="MKVILLGAPGAGKGTQAQFIMNKFGIPQISTGDMFRAAIKESTE
                     LGKQAKALMDEGKLVPDELTVALVKDRIAQPDCANGFLLDGFPRTIPQADALKESGVK
                     IDLVLEFDVADEVIVERMSGRRVHQPSGRTYHVVYNPPKVEGKDDVTGEDLIIRQDDK
                     PETVLERLAIYHKQTKPLIAYYTAEAEAGNTRYERLDGTKPVEEVSAELAKILS"
     gene            complement(6726..7541)
                     /locus_tag="PXD66_001548"
     CDS             complement(6726..7541)
                     /locus_tag="PXD66_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDL6319839.1"
                     /translation="MSYKVVFSLLFSLFLSTANANWTKINDVNYTWGPFNIYHITLFT
                     ESGTYVEGTRPLMLTLQYKKPWEGRDFAVSLARTWNNLGIKLPEKELDEVITHLRKTF
                     PDIKPEDTLHYIALEDRGYFILNDKVVSDVFNKAFNDAIVAIWLDPKMDISHQLLDPS
                     YKPRAEAESIKPQVEERVAENLQEPIVNTEVSKELKADSQAVEQAPNKIDIPVADKTQ
                     ASVVNEATQPVENKDKKEPEKLEEKPKEMKIENPEVFISPIYDDILMLTKYCV"
BASE COUNT         2221 a         1487 c         1485 g         2366 t
ORIGIN      
        1 aaaatttacc ctcacctaaa actaaatgat attgactttc atttataata aaaatatatt
       61 tagcaccgtt ttcataatag attttcatct actcattttc attttgattg atttggtggg
      121 gtaatcccac cttttttatg ctcttaaaac acaacactat gattcaaata aattccatta
      181 gctactctgt tgaacaaaac cgtaaactgc taagcaatat ttccctcaat ttaagcaagg
      241 ggcgtttata tggcgcataa cggctcgggt aaatcaacgt taatgcgttt actaagcgga
      301 gatataacgc ctaccagcgg ttcaatttta gtcgatggat taccacttca tcagctttct
      361 accaaagaag cggcaaaaca tattgcctat ttaccacaac gtttacctga agcggcggat
      421 ttcttagtgc aggaactggt tgctttaggg cgatttccat atcaaaaatg gctacaaaaa
      481 ccaacagaat ttgatgttca aattgttgag caagcaatgg aaagcacctc cgttactcag
      541 tttgctaaac agcctgtaaa tagtctatca ggcggagaaa aacaacgagt ttggcttgct
      601 atgtgcttag cccaacaaac accctattta ctgctagatg agccattggc tgcccttgat
      661 attgtttatc aagttgaagt aatgcagctt attcgcaagc tagtgaatga acaaggtcta
      721 actgtcatta tcattattca cgatattaat cttgcctccc aattttgtga tgaaattatt
      781 gcgttaaagc aaggcgaact ctgtattcaa ggttctgttg atgaaattat gcaagaacag
      841 aaattacttg agatcttcgg gctaaaactg cacttgttta accaccctgc aggcttacat
      901 aaagtggcgg tattatgatg tttaggcaat acaagcggtt catttttctc tttattttgc
      961 aaatttctac cgcatttgct caacctaata tttttacacc agattggtct attgcctctg
     1021 cccttactga aatgggaaat ccgcccattg cgatggggga taagcgaatt tatccgctat
     1081 ggtcgcaccg ccctattcag cctaatagcg tgttagatat tggagcgcgt tatcagccta
     1141 atcgcgaatt attagcacag cttcctgttg aattggtgct atcaaacttt ttctacgctc
     1201 acttggcttc tgtttacccg aaagaaattc cacaaataga cgtgctattt gatggtggca
     1261 atactgaacc tcagcaacat tggcatactt accaaaaagc cactcgtatg attggaacag
     1321 agattaaaaa ccctaatgcg gcagaagcat atttaacgaa aactgaacaa acgttggctc
     1381 aatgggggaa agaaatcctt aacagtaatc cagatattga ttactactct gttgttcaat
     1441 ttggctctcc gagagagtta cgcatatatg cgtctaattc aatgttccgt gtagcctttg
     1501 atttgatggg cttaaaacaa gctgatttag gtgtgggaga tcgctgggga aataaactta
     1561 ttcctgtgag tgatttagct aaattacccg aacgcacctg tttaattgtc atctcacctt
     1621 tttctaaaat gacgcaagca gatttagaaa atagctacat ttggcaacgt ttaggatttg
     1681 gtaaaaaccg ttgtatgaaa gtattaccgc ctgtttggct ctttggtggg gctgattcca
     1741 tctcgcactt tgctgagttt ttacacaatg caatgacaca aggagaaaaa gagtaatgaa
     1801 atcatcattc ttattgattg gctgtattct tttatcctta gtaacagctg ttatccgttt
     1861 aaatatggtc tgggagtggg aatggcagtt actactcata aatgttgatt tattgccaat
     1921 taatcaagct gcggtaaaag cagctctttt acctgaaatg acaacgtcat ttttagcagg
     1981 cggtttattg gctatagcca gcagtggctt acaacatatt gtcagaaata atttagcgtc
     2041 agatagcaca cttgccgtca gtagcggggc tcaacttgca ttgatgattg cggttatctt
     2101 tttcccagcc gccgagcttt tttctagttt ctggattgcg ttacttggct ctttagctgc
     2161 tatcgtactg gttttattta ttgctaaagc ctctaagctc aatcccctta cccttatttt
     2221 agggggattg attgttaata ttttgtttgg agctattgcc tctttactga cccttttcta
     2281 ctttgatttt ttatttggcg tgatggtttg gggaagtggc tcacttttac aagatggttg
     2341 ggcaacttct attaccttag cgataacggt agtagttgca ttttttatct ttgtcttact
     2401 ctctcgtcca ctcacaattt taagtttaga tgatgaacaa gctcgccgct taggcgcgcc
     2461 tgttaattta ctacgctact tagttatttt tgtttgtgcc gccattacag cattggttgt
     2521 aagcaaaata ggtgtgattg gctttattgg atttgcagtg caagtttggc tcattttgcc
     2581 aaaatacgac atttattact tcgcctcttg attgcttttc tagcgggcgg atttttactc
     2641 ttttttacca ataatctcat cggcattgtt agcagaattt acgacactct actacctgtt
     2701 ggtgcattta ccgcaacact aggcgcacca ttattaattt tcttggtatt acgccaacgt
     2761 aaagcacaat ttgaagcaag caatacagca tacttccccg ttaaacgcct caattcgtgg
     2821 aaactgccaa tattgttgtt atgcgctgga gttatcctat tttgtctatt acaaatcatt
     2881 gctccaacaa tgaatggctg ggctattagc attgatgaaa acctgatgag ttcctaccgc
     2941 ttgcctcgta gtctaagtgc gatagcaaca ggggcaatgc ttgcggtaag tggtagccta
     3001 ttacaaatct taacgagaaa cccaatggca agccctgaag tattaggtgt gagttcaggg
     3061 gcaactattg ctgcagtact gtcattctta cttttcccaa cattatctag cattagcttc
     3121 ttgttatttg gtgtattagg gagtttgact gttctcgctc ttattatgtg gctctctcgt
     3181 aaattacaac catcagcgtt aatgattaca ggtgttgcta ttggagcttt agtcagtggc
     3241 ttaatggcta ttattcaact aagcggcagc cctcgtttaa cggcagttat tagttggctg
     3301 tctggctcaa cctattacgc taatccgaat acagtttgga ttttattatc tgttgcaatc
     3361 ctacttatca gtgccacttt actgtttatc aaacctttac gtttgatctc acttggcgag
     3421 accgtttctt accatttagg gttagaggtt aaaaaagtac aaatgattgt attactttta
     3481 attgccctaa tgagtgccgc ctctacactt gcggtagggc caatgagctt cattgggcta
     3541 atgacccctc atttagctaa aaatttaggt gcaattacac cggagaaaca gatccctata
     3601 tcggcattat taggggcaat gctaatgctc attgccgatt ggctcgggcg ttatctaatt
     3661 ttcccttatg agattggggc aggcattatt gcctctattc tcgggggtat ctatttctta
     3721 ctgttactca aacaacataa ataatttttt attcattact taggagaaat tatgaacacg
     3781 ctatctaaag cgataaaact tgccttatac agttctggta tagggtttat ctcaacagct
     3841 tacgctgaaa cagttactca tcttgatacc gtagaagtaa gtgccgaaac gccaaatgcc
     3901 tcatcggaag aaactggacg ttatgcacag aaaaaagcca aaggctctaa tggaatggca
     3961 ttgagtgcga aagacacgcc acaaagcgta acggctgtaa cgcatcaaca aatcagagat
     4021 caaaatctta atacgattgc caaagcactt gaagcaactc acggtgtttc tgtttcttta
     4081 cttgacagag gacgctattc tttcagtgct cgtggttttg ggattgataa agtcaaggta
     4141 gatggtatgg atctcaaagt aagtaaataa ccaatggaca acgggcgaaa atacaggcaa
     4201 cagcgtgtta tacgaccgag tagaagttgt gcgtggtgct acaggcttaa ctactggggc
     4261 aggcgatcct tctgcaagtg ttaatcttgt tcgtaaacga gccaatagca tttcccgtta
     4321 tactatttta gaagctggaa tcgggcgtta tgctgatttc aatacaatga ttgaccatag
     4381 ccaagcattg aataattcag gatcagtaag aggccgcttt attgcagagc ataaaaacgg
     4441 taatacgttc attaaaaatg aaaaagaacg ttacaccacg ctttatggca atattgaagc
     4501 agacttaact gacactacca aacttgggtt aggtgtaagc tatcaaaaag aaaaacgtga
     4561 tgcagcactt tggggtggtt tacccgcatt ctatagcgac ggtggtgaaa ctaactggag
     4621 cagaagcaaa aatgccagtc cggaatgggc ttactgggat aatgaatccg taaactattt
     4681 tgccgatatt acacaaaaac taggcgaaaa atgggaactt gcattaaaag gcaattaccg
     4741 tgtaggcaaa cataattcag aacttttcta tttcagcggc tcatcactta atcgccaaga
     4801 tggattaggt tggtcgcctt ggccgggtaa atttaaaaat gataatacac aatctaatgt
     4861 gcagcttgat cttaccggtt cattctctgc ttggggatta gaacacgatg cggcaatcgg
     4921 tattcaatac aatcgagcac atcgtaattc ctacgctgcc gataatagca cctttgacgg
     4981 agctttcgac tttaataatt ggaatggcat ttatgctaga ccaaactggg gaagtatgtc
     5041 tgaaaaatac taccaaaact taaaagagtt cggggtgtat gtttctaccc gtttacatct
     5101 tacagataga ctttctatgc ttctaggttc tcgtatttca tcgtaccgca cagaaggcac
     5161 tttctatggt tcaacacaaa acttccacgc taacgatgtt tggacacctt atgcaggatt
     5221 aacctttgat attaatccac aaaataccgt ttatgtaagt tataccgata ttttcaaacc
     5281 acaaagtcaa gtggacatca ataacaaact actctctcct gttacgggcg aaacctacga
     5341 agcaggttgg aaagggagtt atttcaatga caacttgcaa actcaaatta gcgtatttga
     5401 aacaagacaa gataatctag ctcaatctgc cggtattaac cctaatacat tattagccta
     5461 ctacacagcc tctaaaggtg cgaaagttcg aggctttgaa gtagaagcat ctggaaatgt
     5521 aactgaaaaa ttaaaactca gtgcaggata tactcagtgg aaaggcgatg atgcttcagg
     5581 taacccaatt aacacaactc aaccacgcaa acaatttaaa ctctttgcaa cttacgatat
     5641 gaaccgttta gtgcaaggtt taacggtagg agcaggtgta aactggcaaa gcagaattta
     5701 taccaaacac gctacttatg gcgaatatgg tcaaaaagct tacactttag tgaatttgat
     5761 ggctcgttat caatttaatg acaacttctc agcacagctt aacattgata acttattcga
     5821 taaaaaatat cgtaacgccc tcagctttag ccaatatagc tacggtgagc cattctatac
     5881 acgcttaaat ttacgctatg aattctaatt ccgtataaca agcggtcaaa aaattaaaaa
     5941 aatttgcaat tttaggcagt aatcggtaag gttactgcct aagtgatttt attattttta
     6001 gtttaattga ggttttcaaa tgaaagtgat tttattaggt gcgccgggtg cgggcaaagg
     6061 tacgcaagct cagtttatta tgaacaaatt cggtattccg caaatttcaa ccggcgatat
     6121 gttccgtgca gcgattaaag aaagcactga gcttggtaaa caagcaaaag cattgatgga
     6181 cgaaggcaag ttagtgccgg atgaattaac ggtcgcctta gtaaaagatc gtattgctca
     6241 accggattgt gctaatggct tcttattaga cggtttccca cgcaccattc cgcaagctga
     6301 tgcgttaaaa gagtcaggtg taaaaattga tttagtgtta gagtttgatg tagctgatga
     6361 agtcattgtt gagcgtatga gtggtcgtcg tgttcaccaa ccttcagggc gtacttacca
     6421 cgtggtgtac aatccaccga aagtggaagg caaagatgat gtgactggtg aggatttaat
     6481 tatccgccaa gatgacaaac ctgaaacggt attagagcgt ttagcgattt accacaaaca
     6541 aaccaaaccg ttaatcgcct actacaccgc agaagcagaa gccggcaaca ctcgttatga
     6601 gcgtttagat ggaacaaaac cagtggaaga agtaagtgct gagttagcga aaattttatc
     6661 ttaaatggat tattaaaatg aacaagcggt aatatttttc gagaatatta ccgcttgttc
     6721 tttgcttaaa cacaatattt ggtgagcatt aagatatcat cataaatcgg tgaaataaac
     6781 acttccggat tttcaatctt catctccttt ggtttttctt ctagcttttc tggctctttc
     6841 ttatctttat tctctaccgg ttgagtcgct tcatttacca cactagcttg agttttatct
     6901 gctacaggta tatctatttt gtttggtgct tgctctactg cttgagaatc cgctttcagc
     6961 tctttagaaa cttcagtatt tacgataggt tcttgtaagt tttccgctac tctttcctct
     7021 acttgtggct taatactttc tgcctctgct ctcggtttat aagatggatc aagtaattga
     7081 tggctaatat ccattttcgg atcaagccaa attgctacaa tagcatcatt gaaagcttta
     7141 ttaaacacat cagagactac tttatcattt aaaataaaat aacctctatc ttctaatgca
     7201 atatagtgca atgtatcttc aggcttaata tctgggaaag tttttctcag gtgggtaata
     7261 acctcatcta actctttttc aggtagctta atgcctaaat tattccaagt tcttgctaaa
     7321 gaaaccgcaa aatcacgccc ctcccacggt tttttatatt gtagagtaag cattaaagga
     7381 cgtgtacctt ctacataagt accgctctca gtaaaaagcg taatatggta aatattaaat
     7441 ggcccccacg tataattcac atcattaatt tttgtccaat ttgcatttgc tgttgataaa
     7501 aatagagaaa acaacagcga aaagaccact ttatagctca ttatttttgt gtccttaaa
//
LOCUS       DANLXF010000111         7519 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.111, whole genome shotgun sequence.
ACCESSION   DANLXF010000111 DANLXF010000000
VERSION     DANLXF010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7519
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.111"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            61..561
                     /locus_tag="PXD66_001549"
     CDS             61..561
                     /locus_tag="PXD66_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HDL6319840.1"
                     /translation="MQTINEIKEEIYPIFEPIIGTIRLKTYFSYYAVFKDGLMIALYQ
                     NGATYLRIAKKDIEYIRQYPKTCILCDEKIGIQSRKFHYIPQVLIDDTPKFSSLLFGI
                     IEELRTEKYKISQKRATQIRNLPNMNLKLERMLKKIGIHSIHDFTQEGYMSTFIKLIL
                     HRRLTV"
     gene            849..2243
                     /gene="fumC"
                     /locus_tag="PXD66_001550"
     CDS             849..2243
                     /gene="fumC"
                     /locus_tag="PXD66_001550"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005567406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HDL6319841.1"
                     /translation="MEFRIEKDTMGEVQVPADKYWAAQTERSRNNFKIGPEASMPAEI
                     IEAFGYLKKAAAYANHDLGVLAVEKRDLIAKACDEILANKLDDQFPLVIWQTGSGTQS
                     NMNVNEVVANRAHVLNGGKLGEKSIIHPNDDVNKSQSSNDTYPTAMHIAAYKKVVEHT
                     LPCVKRLQKTLAEKAEAFKDVVKIGRTHLMDATPLTLGQEFSAYAAQLAFGIKALENT
                     LPHLRELALGGTAVGTGLNTPQGYDVKVAEYIAKFTGLPFVTAQNKFEALATHDAIVE
                     THGALKQLAVSLYKIANDVRLLASGPRSGIGEILIPENEPGSSIMPGKVNPTQCEAMT
                     MVCAQVLGNDVTISFAGTQGHFQLNVYKPVMAYNFLQSAQLLGDACVSFDEHCAVGIE
                     PNYPRIKQQLDQSLMLVTALNTHIGYENAAKIAKTAHKNGTTLKEEAINLGLVTAEQF
                     DEWVRPEDMVGSLK"
     gene            2370..2480
                     /locus_tag="PXD66_001551"
     CDS             2370..2480
                     /locus_tag="PXD66_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005696979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDL6319842.1"
                     /translation="MKKLILIALVTTLGLSACGVKGPLYFPEQAPKHEQK"
     gene            2585..3835
                     /gene="lysA"
                     /locus_tag="PXD66_001552"
     CDS             2585..3835
                     /gene="lysA"
                     /locus_tag="PXD66_001552"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDL6319843.1"
                     /translation="MNHFEYKNNQLFAEEVAIADIIEQYGTPAYVYSRSTLERHWHAF
                     NNAFGSHPHLVCFAVKSNSNIALLNVMARLGSGFDIVSQGELERVLAAGGEASKVVFS
                     GVAKSHSEIKRALEVGIRCFNIESVSELHRINQVAGELGKIAPISLRVNPDVDAHTHP
                     YISTGLKENKFGVSVTQAREVYRLAKTLPNVKITGMDCHIGSQLTELQPFLDATDRLI
                     ILMEQLREDGIELHHLDLGGGLGVPYNGEEPPHPTEYAKALLEKLKGYENLEIIIEPG
                     RAITANAGILVTKVEYLKQNEDRNFAIVDTGMNDMIRPALYEAYMQITEVDKTLNRDK
                     AVYDVVGPICETSDFLGKQRELAISEGDLIAIRSAGAYGAAMSSTYNSRPQAAEIMVD
                     GNQAHLIKARATFADLWRLEKLLP"
     gene            3849..4478
                     /locus_tag="PXD66_001553"
     CDS             3849..4478
                     /locus_tag="PXD66_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HDL6319844.1"
                     /translation="MKLYGLTGACSFVPHVALEWVKLRANQDYAFQAVSREFIKSAEY
                     LALNPRGNVPLLVDGDLALTQNQAIVHYLDELYPEAKLFGSKTARDKAKAARWLAFFN
                     SDVHKSFVPLFRLPSYAEGNETLTKTIRQQSAEQILEQLAFANAHLENHIFFGEEISV
                     ADAYLYIMLNWCRLLGLDFSHLSQLSAFMQRVEADQGVDNVREQEGLKG"
     gene            4549..5994
                     /gene="tldD"
                     /locus_tag="PXD66_001554"
     CDS             4549..5994
                     /gene="tldD"
                     /locus_tag="PXD66_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HDL6319845.1"
                     /translation="MLNQVSNSLLAPSGLELSHLTQVLDHFSGRHIDYADLYFQLSQD
                     ESWSLEDSIIKEGGFYIDRGFGVRAISGEKTGFAYADQITLKQLEQCAAAARSISPTS
                     GQLSVKNFKKTTACSRYPAINPLETISREQKVELLHLVDKTARMADPRVIQVNANLSA
                     VYEEMLVAATDGTLAADIRPLVRLSVSVLVEQNGKRERGSAGAGGRFGLDWFLAPSET
                     GFSRAESLTKEAVRQALVNLTAIPAPAGTMPIVLGAGWPGILLHEAVGHGLEGDFNRK
                     ESSLFSGKIGQMVTSELCTIVDDGTVPNMRGSITVDDEGVPSQRNVLIENGILKGYMQ
                     DKLNARLMGVAPTGNGRRESYAHLPMPRMTNTYLTEGNHEFEEMIESVEYGLYAPHFS
                     GGQVDITSGKFVFSTAEAYLIEKGKITKPVTGATLIGSGIEAMQQVSMVGKKMELDPG
                     IGTCGKEGQSVPVGVGQPTVKLDKITVGGRG"
     gene            6058..6939
                     /gene="prmA"
                     /locus_tag="PXD66_001555"
     CDS             6058..6939
                     /gene="prmA"
                     /locus_tag="PXD66_001555"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDL6319846.1"
                     /translation="MAWVQIRLNSTDKQAEEISDFLTEIGAVSVTFMDSQDTPIFEPL
                     PGETRLWGNTDVVGLFDAETDMNLIVEALKTSKLVDENFAHKIEQIEDKDWEREWMDN
                     FHPMQFGKRLWICPSWREVPDPNAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLEGK
                     TVIDFGCGSGILAIAALKLGAKQAIGIDIDPQAILASQNNAEANGVAEKLQLFLAKDQ
                     PRELQADVVVANILAGPLKELAPNIITLVKPNGNLGLSGILATQAESVCEAYSEAFTL
                     DPVVEKEEWCRITGRKI"
     gene            complement(7114..7189)
                     /locus_tag="PXD66_001556"
     tRNA            complement(7114..7189)
                     /locus_tag="PXD66_001556"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7154..7156),aa:Trp,seq:cca)
     gene            complement(7255..7331)
                     /locus_tag="PXD66_001557"
     tRNA            complement(7255..7331)
                     /locus_tag="PXD66_001557"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7295..7297),aa:Asp,seq:gtc)
     gene            complement(7342..7457)
                     /gene="rrf"
                     /locus_tag="PXD66_001558"
     rRNA            complement(7342..7457)
                     /gene="rrf"
                     /locus_tag="PXD66_001558"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         2246 a         1671 c         1653 g         1949 t
ORIGIN      
        1 ttaatccata agagtattta ttattaaata agtttcatga ccttacatcg gagtacatat
       61 atgcaaacaa tcaatgaaat taaagaagaa atctacccta tatttgagcc tatcattgga
      121 acaattcggc taaaaaccta cttttcctac tacgcagttt ttaaggatgg attgatgatt
      181 gcattgtatc aaaatggagc aacttattta cgtattgcga aaaaagatat tgaatacatt
      241 cgtcagtatc ctaaaacctg tatcttatgc gatgaaaaaa ttggtataca aagtagaaaa
      301 tttcattata ttccgcaagt tctcatagat gatacaccaa aatttagctc gctactcttt
      361 ggaattatag aggaactccg aacggagaag tacaaaataa gccaaaagcg agctacacaa
      421 atccgaaacc taccaaatat gaatttaaag ttagaaagaa tgttgaaaaa aatagggatt
      481 cattccattc atgattttac tcaagaaggg tatatgtcaa cttttatcaa actgattctc
      541 caccgaagac ttactgttta agctaaacgg agctttaaat caccaatata tttacacctt
      601 cacagatcaa caaaaaaggg aactaatgca agaagcaaat caagcgctat ataattcagg
      661 cctaagaaaa agatttaacg ccctgtagtt aaataatgtg atccaaatca taaaatataa
      721 aaaaaccaat aaggaattgc aattcgcagt tcctttcttt ttcttttata tgctagtatc
      781 accttatacc tctaaattat tagtgcctac tgattttcaa ttaatccaac aatttaggag
      841 agcgcatcat ggaattccgt atcgaaaaag acacaatggg cgaagtgcaa gttcctgccg
      901 ataaatactg ggcggcgcaa accgagcgtt cacgcaataa cttcaaaatc gggccggagg
      961 catcaatgcc ggcggaaatt atcgaggcgt ttggctatct gaaaaaagcg gctgcctatg
     1021 ctaatcacga tttaggcgta ctagcggtcg aaaaacggga tttaattgca aaagcctgtg
     1081 atgaaatttt ggcgaacaaa ctcgatgatc aattcccgct ggtgatttgg caaaccggct
     1141 cgggtacgca gtcaaatatg aacgtgaatg aagtcgtggc aaaccgtgca cacgtgttaa
     1201 acggcggcaa gctgggtgaa aaatcgatta ttcacccgaa tgatgatgtg aataaatccc
     1261 aatcttctaa cgacacctac ccaactgcaa tgcacatcgc tgcttacaaa aaagtggtgg
     1321 aacacaccct accttgtgtg aaacgtctgc aaaaaacctt agcggaaaaa gccgaagcct
     1381 ttaaagatgt ggtcaaaata gggcgtactc acttaatgga tgctaccccg ctcacgctcg
     1441 gtcaagagtt ctctgcctat gcagcacagt tagcattcgg catcaaagca ttggaaaaca
     1501 ccctgccaca cctgcgtgag ctggcactcg gcggcacagc agtcggcacc ggcttgaata
     1561 ccccgcaagg ttacgatgtg aaagtggcgg aatacattgc gaaattcacc ggtttaccgt
     1621 tcgttaccgc ccaaaacaaa tttgaggcat tagcgactca cgatgcgatt gtcgaaaccc
     1681 acggtgcatt aaaacagctt gcggtgtcgc tctacaaaat tgccaacgat gtccgtttat
     1741 tagcctccgg tcctcgttca ggcattggcg aaattctgat tccggaaaac gagccgggtt
     1801 catcaattat gccgggtaaa gtaaacccaa cccaatgcga agcaatgaca atggtatgtg
     1861 cccaagtatt aggcaacgat gtaaccatct cttttgccgg cacgcaaggg catttccaac
     1921 tgaatgtcta taaaccggtg atggcgtaca acttcctgca atcggctcaa ctgctaggcg
     1981 atgcgtgcgt gtcgtttgac gaacactgtg cagtcggcat tgaaccgaac taccctcgca
     2041 ttaaacaaca gctcgaccaa tcgctgatgt tagtgaccgc attaaatact cacattggtt
     2101 atgaaaatgc agcgaaaatc gccaaaactg cccacaaaaa cggcacaaca ctgaaagaag
     2161 aggcgattaa cttaggctta gtcacggcag aacagttcga tgaatgggtt cgtccggaag
     2221 atatggttgg cagcttgaaa taagcccaac ccttctcttg taatctgatt tacacctcaa
     2281 tctaccggta agattgaggt gtaatttttt atagcgaggg tactgatttt gttttagaat
     2341 gaagcatcat tttttaagcg gaaaaaccta tgaaaaaact cattttaatc gcattagtta
     2401 ctactttagg cttaagtgct tgtggggtca aaggcccact ctacttccct gagcaagccc
     2461 ctaaacatga acaaaaataa gcattgcaag cggtctgttt tggctaaaat tttgcaaatt
     2521 tttgtgaaaa aatgaccgct tgttgcctct tttctttcaa aatcccaaac acaacggact
     2581 ttcaatgaac cattttgaat ataaaaacaa ccaacttttt gctgaagaag tcgctattgc
     2641 cgatattatc gagcaatacg gcacgccagc ttatgtttac tccagatcta cccttgaacg
     2701 ccactggcac gcattcaata atgcgtttgg ctcgcaccca catttagttt gctttgcagt
     2761 caaatccaac tctaacattg ccttgttaaa tgtaatggct cgtctcggtt caggctttga
     2821 tattgtctct caaggcgagt tggagcgtgt cttagctgcc ggcggcgaag cctctaaagt
     2881 tgtcttttcc ggtgtggcaa aatcgcacag cgaaatcaaa cgagcattgg aagtcgggat
     2941 tcgttgcttt aacattgaat cggtttccga acttcaccgc attaaccaag ttgccggtga
     3001 actaggcaaa atcgcaccga tttcactgcg ggtaaacccg gatgtggacg cccacactca
     3061 cccatacatt tccaccggtt taaaagaaaa caaattcggc gtgagcgtta ctcaagcccg
     3121 tgaagtgtat cgcttagcca aaaccttacc gaatgtgaaa attaccggta tggattgtca
     3181 catcggttcg caattaaccg aattgcaacc ttttttagat gcaacggatc gcttaatcat
     3241 cttaatggag caactgcgtg aagacggcat tgagctacac cacttagact taggtggcgg
     3301 tttaggcgtg ccttacaacg gcgaagagcc tccgcatccg accgaatatg ccaaagcctt
     3361 gctggaaaag ctcaaaggct atgaaaactt agaaatcatt attgaaccgg ggcgtgccat
     3421 taccgcaaat gccggtattt tagtgacgaa ggtggaatac ttgaaacaaa atgaagatcg
     3481 taattttgcg attgtcgata ccggtatgaa cgatatgatt cgcccggctc tgtatgaagc
     3541 ctatatgcaa attacggaag tggataaaac cttaaaccgt gacaaagctg tttacgatgt
     3601 ggtcggtccg atttgcgaaa cctcggattt cttaggcaaa caaagagaat tggcaatttc
     3661 tgaaggggat ttaatcgcta ttcgttctgc cggtgcttat ggggcggcga tgtcttcaac
     3721 ctacaactct cgcccacaag cggcagaaat tatggttgat ggcaaccaag cccatttaat
     3781 taaagcccga gccacttttg ctgatttatg gcgtttagaa aaattattac cttaatgagg
     3841 aagaaaagat gaaattatat ggattaaccg gtgcttgttc atttgtacca cacgttgccc
     3901 tagagtgggt gaaattgcgt gccaatcaag attatgcgtt tcaagctgtt agccgtgaat
     3961 ttattaaatc ggcggaatat ctggctttaa accctcgtgg taacgtacca ttgttggttg
     4021 atggcgactt ggccctcacg caaaaccaag ctatcgtcca ctatttagac gagctttatc
     4081 cggaagctaa attattcggc agcaaaaccg ctcgtgataa agccaaagcg gctcgttggt
     4141 tagctttttt caattcagat gtgcataaat cattcgtgcc actgttccgc ttgccaagtt
     4201 atgcggaagg caatgaaacg ctcaccaaaa ctattcgcca acaatcggct gagcaaattt
     4261 tagaacaact ggcttttgcg aatgctcatt tagaaaatca tattttcttt ggcgaagaaa
     4321 tttcggttgc cgatgcgtat ctctacatta tgctcaactg gtgtcgccta ctagggttgg
     4381 atttctctca tctttcccaa ctttctgcct ttatgcaacg agtggaggca gaccaaggcg
     4441 tagataatgt gcgtgagcaa gaaggcttaa aaggttaatt caccattgtt catttaagac
     4501 ggtagatttt atgttaaaat ctaccgtctt ttatttttag gaatcaccat gctaaatcag
     4561 gtttcaaaca gcttgcttgc tccaagtggg cttgaattat cgcacttaac tcaagtgtta
     4621 gatcacttct ccggccgtca tattgattat gccgatctct acttccaact cagccaagac
     4681 gaaagctgga gcttggaaga cagcatcatc aaagaaggcg gtttttatat cgaccgaggg
     4741 ttcggtgtgc gagccatatc aggcgaaaaa accggctttg cctacgccga tcaaatcacg
     4801 ttaaaacagc ttgagcaatg tgccgctgcc gcacgttcaa tcagcccaac aagcggtcaa
     4861 ttatcggtaa aaaatttcaa aaaaacgacc gcttgttctc gctacccggc aatcaacccg
     4921 ctagagacca tcagccgtga acaaaaggtc gaattgctcc atttggtgga taaaaccgct
     4981 cgaatggcag atccgagagt gattcaagtg aatgcgaatc tctcagcggt atatgaagaa
     5041 atgttagtgg cagcgactga cggcacactg gctgcggata ttcgcccgtt agtgcgttta
     5101 tctgtgtcgg tactggtgga acaaaatggc aagcgtgaac gaggttcagc aggtgccggt
     5161 ggtcgctttg gtttagattg gttcttagca ccaagcgaaa ccggctttag ccgtgcggaa
     5221 tcgcttacca aagaagccgt tcgccaagcc ctcgttaatc tcacagccat tcctgcaccg
     5281 gcaggcacga tgccgattgt tctcggtgct ggctggccgg gcattttgct ccacgaagca
     5341 gtcggacacg gcttggaggg tgattttaac cgtaaggaat cttcgctctt cagtggtaaa
     5401 atcgggcaaa tggtcacctc tgagctttgc accattgttg atgacggtac ggtgccgaat
     5461 atgcgaggct caatcacggt agatgacgaa ggcgtgcctt cgcaacgtaa tgtgttaatt
     5521 gaaaacggca ttctaaaagg ctatatgcaa gacaaactca atgcccgcct gatgggagtt
     5581 gccccaaccg gcaacggcag acgggaatct tacgctcatt tgccgatgcc acgaatgacc
     5641 aacacctacc tcaccgaagg caatcacgaa tttgaagaga tgattgaatc ggtcgaatac
     5701 ggtttatatg ctcctcactt cagtggtgga caagtggata ttacctccgg caaatttgtg
     5761 ttctccaccg ccgaagccta cttaatcgaa aaaggcaaaa tcaccaaacc tgttaccggt
     5821 gcaaccctga tcggcagcgg catagaagca atgcaacaag tttcaatggt cggcaaaaaa
     5881 atggaactcg accccggcat cggcacttgt ggcaaagaag ggcaatccgt accagtcggc
     5941 gtagggcagc ctacagtgaa gttggataaa attacggtgg ggggaagagg gtaatctctc
     6001 cccgtttgca aaatataaag aaaatatcac cgcttgtagc gataaaagga aaacaaaatg
     6061 gcttgggtac aaattcgttt aaacagtaca gataaacagg ctgaggaaat cagcgatttt
     6121 ttaaccgaga ttggagcggt gtcagtgact tttatggata gtcaagatac cccaattttt
     6181 gaacctcttc cgggggaaac ccgtctttgg ggaaataccg atgtggttgg gcttttcgat
     6241 gcggaaactg atatgaatct tatcgtagaa gcactaaaaa ccagcaaatt ggtggacgaa
     6301 aactttgctc acaaaatcga gcaaatcgaa gacaaagact gggaacggga gtggatggat
     6361 aatttccacc caatgcagtt tggcaaacgg ttatggatct gcccaagctg gcgtgaagta
     6421 ccagacccga atgcagtgaa tgttatgtta gaccctggtt tagcatttgg caccggcaca
     6481 cacccaacga cctcgctttg cttacaatgg ttagatagct tagatttaga aggcaaaacg
     6541 gttatcgact tcggctgtgg ctccggtatt ttagctatcg ctgcacttaa actcggtgca
     6601 aaacaagcca tcgggattga tatcgaccca caggcgattt tagctagtca aaacaatgct
     6661 gaggcgaacg gcgtagcaga aaaactacaa ctattcttag ccaaagatca gccaagagag
     6721 ttacaagccg atgtcgttgt tgcgaatatt ctagccggcc cactcaaaga gttagcaccg
     6781 aatatcatca cgctggttaa accaaacggc aatttaggct tatccggcat tcttgctact
     6841 caggctgaaa gtgtgtgtga ggcttatagc gaagccttta cgctagaccc tgttgtagaa
     6901 aaagaagaat ggtgtcgtat tacaggccgc aaaatctaat ataagaagcc ctgttttaaa
     6961 cagggcttac tttttgataa attctactac tctttacctg tcttgggaat cgttctccct
     7021 aaattaatta acacaagcgg tagaaataac aggaaaattt acaaattaca taaatgaaaa
     7081 aagccgactg aaatcagtcg gctttctaaa taatggcagg ggcggagagg ctcgaactcc
     7141 caacacccgg ttttggagac cggtgctcta ccaattgaac tacgccccta ttgataggta
     7201 ttcattttgc acttataggg gcgaattact attcgcccct gcattaaaga ttattggcgg
     7261 aacggacggg actcgaaccc gcgaccccct gcgtgacagg caggtattct aaccagctga
     7321 actaccgctc ctaaattaat agattgataa tgccctactc tcacatggcg aatcaccaca
     7381 ctaccatcgg cgttactgcg ttttacttct gagttcggta tggagtcagg tagagccaca
     7441 gcactctggt tatcaatcaa attctttcat ttagtcagtt tactaaaata tagtttatac
     7501 tttctaataa actcactaa
//
LOCUS       DANLXF010000112         7495 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.112, whole genome shotgun sequence.
ACCESSION   DANLXF010000112 DANLXF010000000
VERSION     DANLXF010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7495
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.112"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            18..1031
                     /gene="cas1c"
                     /locus_tag="PXD66_001559"
     CDS             18..1031
                     /gene="cas1c"
                     /locus_tag="PXD66_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003762781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1"
                     /protein_id="HDL6319847.1"
                     /translation="MRKLQNTLYITTQGSYLHKERETLVVEQDRKKVAQLPIHSIGHI
                     FCFGNVLVSPFLMGFCGENNVNLAFFTENGRYLGRLQGRQSGNVLLRRAQYKKSETNP
                     VPVARNIIAAKIQASKRVLQRQIRNHGENADLQSTITALNYSLQQLKTADNLDLIRGI
                     EGDAAARYFGVFSHLIKENSGFHFDGRNRRPPRDGVNALLSFLYSIVGKDISGALQGV
                     GLDPQIGFLHADRSGRDSLAQDILEEFRAWWVDRMVLSLINRGQVKPDDFVTEASGAV
                     TLKPEARKLLFQTLQAKKQEKIIHPFLEEEVEIGLLPYIQAMLLARHLRGDLAEYPPF
                     LMR"
     gene            1098..1391
                     /gene="cas2"
                     /locus_tag="PXD66_001560"
     CDS             1098..1391
                     /gene="cas2"
                     /locus_tag="PXD66_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005540845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HDL6319848.1"
                     /translation="MLMLITYDISFDDPEGQARLRRIAKHCLDYGVRAQYSVFECDVT
                     PAQWVTLKNKLLATYNPDCDSLRFYHLGSKWRNKVEHHGAKPSVDIFKDTLIL"
     repeat_region   1583..2617
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1583..1602
                     /rpt_unit_seq="cagccacctacgcgtggctg"
     gene            2867..4342
                     /gene="rng"
                     /locus_tag="PXD66_001561"
     CDS             2867..4342
                     /gene="rng"
                     /locus_tag="PXD66_001561"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HDL6319849.1"
                     /translation="MSSTELLVNVTPSETRVALVENGILKELHIEREAKRGIVGNIYK
                     GRVTRVLPGMQSAFVDIGLEKAAFLHASDIVSHTECVDENEKKQFLVKDISELVREGQ
                     DIVVQVVKDPIGTKGARLTTDITLPSRYLVFMPENSHVGVSQRIESEEERARLKSLVE
                     PYCDELGGFIVRTAAEEATEESLKQDVEFLKRLWRKVLERKGKYSAKSMLYGELALAQ
                     RVLRDFIGTHINSVRIDSKLTYEQVREFLAEFMPELTDCVTLYTGQQTLFDAYGVEKA
                     IQKALDKRVDLKSGGYLIIEQTEAMTTIDINTGAFVGHRNLAHTIFNTNIEAIQAIAH
                     QLQLRNLGGIIIIDFIDMQEEEHRERVLQSLQEALKGDRVKTNVNGFTQLGLVEMTRK
                     RTRESLERILCCDCQACQGRGTVKTVETVCYEIMREIVRVHHLFKTENIHVYAAKDVA
                     EYLINEESHALLAELQAFIGKKIEIKLEPYYHQEQFDVVVM"
     gene            4496..5998
                     /gene="lysS"
                     /locus_tag="PXD66_001562"
     CDS             4496..5998
                     /gene="lysS"
                     /locus_tag="PXD66_001562"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDL6319850.1"
                     /translation="MSEVENQELDLNGEMLARREKLNKIREQGNAFPNTFRRDALAKD
                     LHAQYDAVEGETLKEQNIQVKVAGRIMLKRVMGKASFFTIQDVSGAIQLYVARDNLSD
                     SVYEEKVSLWDLGDIVGVEGALFKTKTGELTVRCSEVQLLTKSLRPLPDKHHGLTDLE
                     TRYRQRYLDLISNEESRRTFMIRSQVVSGIRKFFLERDFIEVETPMLQVIPGGAAAKP
                     FITHHNALDVDMYLRIAPELYLKRLVVGGFERVFELNRNFRNEGVSVRHNPEFTMIEY
                     YQAYADYHDLMDNTEELLRQLALDILGTTDVPYGDYVFDFGKPFERITMHDAIVKYGN
                     GIQREDLDSFEKSVEIAKGLGIEIQKSWGLGSVVNAIFEEVAEHQLIQPTFLMAHPAE
                     ISPLARRNDENPEVTDRFELFIGGREIGNGFSELNDAEDQAERFDAQVAAKDAGDDEA
                     MFKDEDFIVALEHGLPPTAGEGLGIDRLVMIFANAPSIRDVILFPAMRQK"
     gene            complement(6076..7149)
                     /locus_tag="PXD66_001563"
     CDS             complement(6076..7149)
                     /locus_tag="PXD66_001563"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HDL6319851.1"
                     /translation="MAQQITLFEDVPLPLTLGGELSPITVAYQTYGRLNAEKSNAVLL
                     CHALTGDAKPYYDSPTEKGWWQDFIGKGLAFDTDKYFFICSNVLGGCKGTTGPASINP
                     KTNKPYGSLFPIITVQDIVNVQKALLDYLEIPQLHAVVGGSFGGMQATQWGISYPDKV
                     RNIINLCSSLTLSAEAIGFNHVMRQAIINDPHFNGGNYYDGIAPDNGLKIARMLGMLT
                     YRTDIQLAKAFGRETKQQGQIWGDHFQVESYLSYQGTKFLGRFDANTYLRLLRALDLY
                     DPALGYENEETALKRIKANYTFVAVTSDQLFKQVDVYKSKQKLEQAGVNLEFHEFHSD
                     FGHDAFLVDYDFFEPMIKAGLAK"
BASE COUNT         2211 a         1385 c         1705 g         2194 t
ORIGIN      
        1 taaagggatt ttcaaccatg cgcaaactac aaaacaccct ctacattacc acccaaggta
       61 gctatttgca caaagagcga gaaaccttgg tggtggagca agatcggaag aaagtggcac
      121 agttgccgat tcattccatt gggcatattt tttgttttgg caatgtgtta gtgtcgccgt
      181 ttttaatggg attttgtggc gaaaataacg taaatttagc gttttttacc gaaaatgggc
      241 gttatcttgg gcggttgcag gggcggcaaa gtggtaatgt attgttacgc cgagcccagt
      301 ataaaaagtc ggagaccaac cctgttcctg tggctcgcaa catcattgca gccaaaattc
      361 aagcatctaa gcgggtgtta caacggcaaa ttcgtaatca cggcgaaaat gctgaccttc
      421 aatcgacaat taccgcatta aattacagcc tgcaacagtt gaaaacagca gacaatttgg
      481 atttgattcg tggcattgag ggcgatgctg cggctcgtta ttttggggtg ttttctcatc
      541 ttatcaaaga aaatagcggt ttccactttg acggacgtaa tcgccgccca cctcgtgacg
      601 gtgtgaatgc gttgctttca tttctttata gcattgtagg gaaagatatt agcggggcat
      661 tgcaaggcgt ggggcttgat ccccagattg gctttttgca tgcagatcgt tcggggcgag
      721 atagcttagc tcaagatatt ttggaggaat ttcgagcgtg gtgggtggac agaatggtgc
      781 tgtcgctgat caatcgaggg caggttaagc ctgatgattt tgtgacggaa gccagtggtg
      841 cggtaacgct caagcctgaa gccagaaaat tactatttca aaccttgcaa gcgaaaaaac
      901 aagaaaaaat catccaccca tttttagagg aagaagtgga aatcggcttg ttaccttata
      961 tccaagcgat gttattggct cgtcatttgc gtggcgattt ggcagaatat ccgccgtttt
     1021 taatgcgtta aaatctgcaa aattttgttg aaatttgacc gcttgtttat gttttattca
     1081 ttgaggaatt agcaactatg ctgatgttga ttacctacga catttcattt gatgaccccg
     1141 aagggcaggc tcgcctacgc cgtattgcta agcattgttt ggattatggt gtgcgggcgc
     1201 aatattctgt gtttgagtgt gatgttactc ctgctcagtg ggtaacattg aaaaataaat
     1261 tattggcaac ttacaaccca gactgtgata gtttgcgctt ttaccattta ggcagtaaat
     1321 ggaggaataa agttgagcac catggcgcaa aaccttcagt ggatattttt aaagatacgc
     1381 tgattttata aatcgctagc ctgtagttct cataaaaatg tagcaaggtt agcgaatcta
     1441 tattgttctt taataatcaa ggaattagaa aaaataaggc acaatatatt ggcgatatgc
     1501 tatactttat ccactctcta caaaacgtta gcgaataaag ggctaaaaag cctgatattt
     1561 caatacgtta tcttataggg accagccacc tacgcgtggc tgcgaattga aaccctataa
     1621 cgagcaatgg cattttgatc tacggtaaac cagccaccta cgcgtggctg cgaattgaaa
     1681 cttcttgaat acctccggag tcagttaaga taaggccagc cacctacgcg tggctgcgaa
     1741 ttgaaacctt tttcaagaat caagattaag agatccaaac aaccagccac ctacgcgtgg
     1801 ctgcgaattg aaaccaccgc tgctacttcg gttatcggag tagtttgcac ccagccacct
     1861 acgcgtggct gcgaattgaa acgtggcaca gtaacacgag cagatgctga taaacgccca
     1921 gccacctacg cgtggctgcg aattgaaacc agcagccgtt gttaccgtgg tgcaatgctt
     1981 gcgccagcca cctacgcgtg gctgcgaatt gaaactgcga tttttggatt tttcgggcga
     2041 taaatgatac cagccaccta cgcgtggctg tgaattgaaa ctcgtggctg gttatacaaa
     2101 gaaaagcatt gttgggcagc cacctacgcg tggctgtgaa ttgaaactta aatctaaagc
     2161 taaagctatt ttattaatta aagcagccac ctacgcgtgg ctgtgaattg aaactttacc
     2221 gtttaccgat tggattttaa tgctagaggc agccacctac gcgtggctgt gaattgaaac
     2281 cctaaattgc cgtaaaaatc tgccattagc tacgcgcagc cacctacgcg tggctgtgaa
     2341 ttgaaacctc ataacgaatc caatcctgtt cggttaatcc ccgcagccac ctacgcgtgg
     2401 ctgtgaattg aaacaggggc gtgtaccgca atcaatttta attataacca agcagccacc
     2461 tacgcgtggc tgtgaattga aactttatct gttgcggtca attgtatatc ttgaatttgc
     2521 agccacctac gcgtggctgt gaattgaaac ctcgtcttga attggaaaat caagggcgac
     2581 aataggcagc caccttcgcg tggctgtgta ttgaaacggt gtacccacaa atcctatgtt
     2641 ttcgccattg gctgctacct tcgcatcaat atatattgaa atatcaatgg caaatttgta
     2701 tgttgttaat tagactgatt gcatctgcta tgtatcatag taatgacgaa aatattctat
     2761 tttgcaaaaa ataatcagaa attacccgct tgttcgaact gagtttttgt tttctaagat
     2821 gtgaaatgat acactttaga tagttttaaa atatagagac agtaagatga gtagtacgga
     2881 attattagtg aatgttaccc caagcgaaac aagggtagcg ttggtagaaa acggaatatt
     2941 gaaagaatta catattgagc gggaagctaa acgcgggatt gttggtaata tttataaggg
     3001 aagggtgaca agggtattac caggtatgca atctgctttt gtggatatcg gcttggagaa
     3061 ggctgctttt ttgcacgctt ctgacatcgt ttcgcatacg gaatgtgttg atgaaaatga
     3121 aaagaagcaa tttctggtta aagatatttc tgaattggtg cgtgaagggc aagatatagt
     3181 tgtacaagta gtgaaagatc caattggtac taaaggagct aggctcacta ctgatattac
     3241 gttaccatct cgttatttag tgtttatgcc agaaaatagc catgtgggag tatctcagcg
     3301 gattgaaagt gaagaagagc gtgctcgttt aaaaagcttg gttgagcctt attgtgatga
     3361 acttgggggc tttattgtac gtaccgctgc agaagaggca actgaagaaa gtttaaagca
     3421 agatgttgaa tttttaaaac ggttgtggcg taaagtatta gaacgaaaag ggaaatattc
     3481 cgcaaaatca atgctttatg gagagcttgc gcttgctcaa agggtactgc gtgattttat
     3541 tggtacacat atcaattcgg ttagaatcga ttcaaagcta acttatgagc aagtgcgaga
     3601 atttttagct gagtttatgc cagaattaac ggattgtgtc accctctata ccggtcagca
     3661 aactttattt gatgcctatg gtgtagaaaa ggcaatccaa aaggcattgg ataaacgggt
     3721 tgatttaaaa tcggggggct atttaattat tgagcaaact gaggcgatga cgactattga
     3781 tattaatact ggtgcttttg tcggacatcg taaccttgca catacgattt tcaacacaaa
     3841 tattgaagca atacaggcaa ttgcacacca gctacagttg cgtaatttag gtggtattat
     3901 cattattgat tttattgata tgcaagagga agaacatcgt gagcgcgttt tacaatcgtt
     3961 acaagaggca ttaaaaggcg atcgtgttaa aaccaatgtg aatggcttta ctcagcttgg
     4021 cttggtggag atgaccagaa aacgtacaag ggagagtcta gagcgaattc tttgttgcga
     4081 ttgccaagct tgccaagggc gtggcacagt gaaaactgta gagacggttt gctatgaaat
     4141 tatgcgtgaa attgtgcgtg tacatcattt gtttaagaca gaaaatattc acgtttatgc
     4201 agctaaagat gttgctgaat atttaattaa cgaggaaagc cacgcattat tggctgagct
     4261 tcaggcgttt atcggtaaaa aaattgagat taaacttgag ccatattatc atcaagagca
     4321 gtttgatgtg gtcgtgatgt aatctattac ttggttgagg ctaaatagtt agcctcaata
     4381 tcgttagtta caaaaaattt agaaaatctg accgcttgtt tcctatccaa attcctcaaa
     4441 ataacgtata atcgtccagt tttatttatc cttttttgaa atctaaggta attcaatgtc
     4501 tgaagtagaa aatcaagaat tagatctaaa cggtgaaatg ttagcccgcc gtgaaaaatt
     4561 aaacaaaatc cgtgagcagg gtaacgcatt tccaaataca ttccgtcgtg atgcgttagc
     4621 caaagatctg cacgctcaat atgatgcagt ggaaggtgaa accttaaaag aacagaatat
     4681 tcaagtgaaa gttgccggtc gtattatgtt aaaacgagta atgggtaagg catctttctt
     4741 taccattcaa gatgtgagcg gtgcgattca gctttatgta gctcgtgata acttaagtga
     4801 cagcgtttac gaagaaaaag tcagcctttg ggatttaggc gacattgtgg gcgtcgaagg
     4861 tgcgttattt aaaaccaaaa ccggtgaatt aacggttcgt tgctctgaag ttcaactttt
     4921 aaccaagtct ttacgcccgt tgccggataa acatcacggt ttaacggatt tagaaactcg
     4981 ttatcgtcag cgttatttag acttgatttc taatgaagaa tctcgccgta cttttatgat
     5041 ccgctcgcaa gtagtttcag gcattcgcaa attcttctta gaaagagact ttattgaagt
     5101 tgaaacgcca atgttgcaag tgatcccggg cggtgcggca gcgaaaccgt ttatcactca
     5161 tcataatgcg ttagatgtgg atatgtatct gcgtattgct ccagagcttt acttaaaacg
     5221 tttagttgtg ggtggttttg agcgagtatt tgagcttaac cgtaacttcc gtaacgaagg
     5281 ggtttcagta cgccacaatc ctgaatttac aatgattgag tactaccaag cctatgcgga
     5341 ttatcacgat ttaatggata ataccgaaga actgttacgc caattagcct tagatattct
     5401 tggcacaact gacgtacctt atggtgatta tgtattcgat ttcggtaaac cgtttgagcg
     5461 tattacgatg cacgatgcga ttgtgaaata tggtaacggc attcaacgtg aagatttaga
     5521 cagcttcgag aaaagtgtgg aaattgcgaa aggcttaggc attgaaattc aaaaatcttg
     5581 gggtttaggc tcggtagtca atgcgatttt tgaagaagtg gcagaacatc aattaattca
     5641 gccaactttc ttgatggctc acccggcaga aatttcacca ttagctcgcc gtaatgatga
     5701 aaacccagag gtaactgatc gttttgagtt atttatcggt ggtcgtgaaa ttggtaatgg
     5761 tttctcagaa ttaaacgatg cagaagacca agcagagcgt tttgatgctc aagttgcggc
     5821 gaaagatgcc ggtgatgatg aagcaatgtt taaagatgaa gactttatcg tggcattgga
     5881 acacggtctg ccgccaacag cgggtgaagg tttaggaatt gaccgcttag taatgatttt
     5941 cgctaacgcc ccatcaatcc gtgatgtaat tttattccca gcgatgcgtc agaagtaatc
     6001 agaaatagaa caaaagcaca cttcggtgtg cttttttgta ttacaagcgg tcaaattttt
     6061 aataaaaaat gcaatttatt ttgctaatcc ggctttaatc atcggttcaa aaaagtcata
     6121 atcaaccaaa aaggcatcgt gtccgaaatc ggagtgaaac tcgtggaact ccaaatttac
     6181 gcctgcctgt tctaattttt gtttgctctt atacacgtca acttgcttga atagttggtc
     6241 gcttgttacc gctacaaaag tgtagttggc tttaatacgt tttagggcgg tttcttcatt
     6301 ttcatagcca agagccgggt cgtataaatc taatgctcgg agtaaacgga ggtaagtgtt
     6361 ggcatcgaaa cgacctaaaa atttcgtgcc ttgatagctt aaataggatt ccacttggaa
     6421 atggtcgccc caaatttgtc cttgctgttt ggtttctcgt ccaaaggctt tagcaagttg
     6481 aatatcagta cggtaagtca gcataccgag catacgggcg atttttaagc cgttatcagg
     6541 tgcaatgcca tcataatagt tcccaccgtt aaagtgtgga tcgttaataa tagcttggcg
     6601 cataacgtgg ttaaaaccga tagcctcggc acttagggta agtgacgaac aaagatttat
     6661 aatgttgcga actttatcag ggtaactaat accccactgt gttgcttgca ttccgccgaa
     6721 agatccccct accactgcgt gaagttgagg gatttctaaa tagtccaata atgctttttg
     6781 tacattaaca atatcttgca ctgtaataat tggaaacaaa ctaccgtaag gtttattggt
     6841 tttagggtta atcgaagctg ggccggttgt gcctttacaa ccgcctaata cattactgca
     6901 aataaagaaa tatttatcgg tatcaaacgc aagccctttg ccgatgaaat cttgccacca
     6961 gcctttctcg gttggtgagt catagtaagg cttagcatca ccggtaaggg cgtggcaaag
     7021 caagaccgca ttgcttttct ctgcatttaa tctgccgtaa gtctggtaag ccactgtgat
     7081 aggggacagt tcgcctccta atgtgagtgg taaaggtacg tcctcaaaaa gggtaatctg
     7141 ctgagccata aaaatcctgt ttgcaaaaaa ttctgctttt ttgaccgctt gttgtaatga
     7201 ccgttaaatt agcggtgttc atttcaattg tcaagaattt ttagacgtct aaacgtctag
     7261 atttttaaac ggctaaaatc aaaattttat tgctgagaag atatttatta cttaacaaat
     7321 aacatgaaat agaataaatt tattgaacga ttagactatt ttgtttgtct ctaatttacc
     7381 catcttaaat agggtagcaa aagtaaagga tctcatttga accaaacgcg tgataggtta
     7441 gaaaaattgc cacttttcca taaaatgcag cagtttatta ttaaggcatt gaaaa
//
LOCUS       DANLXF010000113         7463 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.113, whole genome shotgun sequence.
ACCESSION   DANLXF010000113 DANLXF010000000
VERSION     DANLXF010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7463
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.113"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            16..729
                     /locus_tag="PXD66_001564"
     CDS             16..729
                     /locus_tag="PXD66_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL6319852.1"
                     /translation="MILPPFNIILLWLLALILFSLGYKYAAYFFALLGILILYIFSIP
                     YTSQKLGDSLVEAEPFTIEKYKNAQAIVVLGGGLRDSQELFGKLAITGIPLERMRYAA
                     YLHKETGLPILATGSSPNGTSEAKVMADEFKMFFNIETKWLENKAKTTKENAIFSREI
                     LEKENIHKIILVTNQWHMKRAEMLFSQQGFEVMSASVGSGDTPDHYKLNFMHFIPQAG
                     AMNSNMLALKEWIGYWKEK"
     gene            complement(806..3004)
                     /gene="priA"
                     /locus_tag="PXD66_001565"
     CDS             complement(806..3004)
                     /gene="priA"
                     /locus_tag="PXD66_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDL6319853.1"
                     /translation="MALNVPLHRYFDYLLPPAFNACQGGRVVVPFGRQTKIGIVVDFP
                     QTSDVPTEKLKPIKAALDSSPIFDSEMWQLLNWAARYYHAPIGEVLTGALPIKLRNGD
                     STERVQPNYFVVTEQGRKSLIIGENKKAKKQVERLTELAECAKYFEKPTACSPSAWKG
                     LLEKGYIEKIDVPFMAQAWQDSLGEFPLVNTNNRLILNKQQTLVVSRLNAQTTFASFL
                     LNGVTGSGKTEVYLQLIETVLKREEQVLLLVPEIGLTPQTVQRFKARFNVEIDVLHSN
                     MNETERLNAWLRAKNGESAIVIGTRSALFTQFQKLGVIILDEEHDSSFKQQDGWRYHA
                     RDLAVVRAKNANIPIVLGSATPSLESLHNVQNGKFIELTLTARAGNAQLANQQIIDLK
                     TQRVTAGLSERLLSMMKTQLEQGNQVMLFLNRRGFAPVLLCHECGWVCECEACNKPFT
                     YHQKQKVLRCHHCATQRVIPRQCGHCGSTHLITTGVGTEQLEQVLNQQFPHYQVTRID
                     RDSTARKGALENHLTAIREGKSQILIGTQMLAKGHHFPNVTLVAIVNVDSALFSTDFR
                     AEEKLAQLYIQVAGRAGRAEKKGEVVLQTHYPDHPLLKTLLEQGYRAFADEALKMRQL
                     MSLPPFSSQVLFKATGKDNQQTLQLLEQLADYFSQKIQQQNGQGFQLPPPFSAPMAKK
                     AGHYRWLLLIQHPLRGALQQLLDAFDQERESLNIPNNIRLNIDIDPQEIS"
     gene            complement(3016..3628)
                     /locus_tag="PXD66_001566"
                     /pseudo
     CDS             complement(3016..3628)
                     /locus_tag="PXD66_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818858.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
     gene            complement(3621..4520)
                     /gene="rarD"
                     /locus_tag="PXD66_001567"
     CDS             complement(3621..4520)
                     /gene="rarD"
                     /locus_tag="PXD66_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDL6319854.1"
                     /translation="MLKGILLSLSASVLFGCMYYLAIFLKPLAGVSIFGIRMLVTIPF
                     LFLALYLFKKQNEFWVFLQRLKQEPYLLLVILITASIVGGQMWLFLWAPNSGKAIEVS
                     MGYLLMPIIMVAFGKVVYKETLSLNKWLAIVVAFIGVASNILFTGKISIASLFVCTGY
                     PIYFYLRRKFGLSHLHSFVVEILYLLPVACYFISQTDMQYVETQNLNIYYFIALLGLI
                     SGTALISYTLASTILPFNLLGLLGYVEPLLMLVVSFLIGERLANESYLLMICLLISIS
                     FLVLDGMFALRQQRKRKLSVNNE"
     gene            complement(4747..5964)
                     /locus_tag="PXD66_001568"
     CDS             complement(4747..5964)
                     /locus_tag="PXD66_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL6319855.1"
                     /translation="MDRFPKSDKLAQVRYDIRGPIHKEALRLEEEGHKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIVQYYQSKGMRGMDVNDVYIGN
                     GVSELITMSMQALLNDGDEILIPMPDYPLWTAASTLAGGKAVHYLCDEENEWFPDLED
                     IKSKITPRTKGILVINPNNPTGAVYSRQILLEIAELARQHNLIIFADEIYEKIVYDGA
                     VHHHIAALAPDVLTVTYNGLSKAYRVAGFRQGWMVLSGPKHQAKGFIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFVLPGGRLLEQRNKMHELLVQIPGISCVKAKGALYM
                     FPKIDTEMYGIKDDQKFIYDLLRQEKVLLVQGSGFNWKKPDHFRVVTLPYAHQIEEAI
                     GRLANFLKTYRQD"
     gene            6152..7456
                     /locus_tag="PXD66_001569"
     CDS             6152..7456
                     /locus_tag="PXD66_001569"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HDL6319856.1"
                     /translation="MLIFHQLKQELTKSYSKVGREQGLVAIQASMPLLEANIPLLGWL
                     KTQKTSYPHFFLQYRDTDQTIATIGTLRQFYTLEAAQSFIQQHHFPLIGGLQFEGNTQ
                     FILPQFSLVKNQQNLTACFYFDSDNFEQQAVIFEQFLANFSQITELGLTDNPLVSMSA
                     TSHFEQWKANIEKAIVSIEQGDFQKVVLANAMRLHFEQPICAYSLLAQSQKTNLGCYH
                     FLWAESADVAFLGSTPERLYQRQGLHFSTEALAGTAAVTTSEVQTELNAQWLLSDPKN
                     IYENQLVVDDIENQLKDCVTEFAVSEAKIKRLHNVQHLRRQIQATLKPDTSDSDCLSK
                     IHPTAAVAGLPRQKAKGFITENEPFTRHWYAGTLGVMGNEEAEFCVTLRSALISQNSI
                     TLYAGAGIVSGSDPQSEWQEIKRKAQGMAKLLDVDLVTKGEL"
BASE COUNT         2253 a         1641 c         1482 g         2087 t
ORIGIN      
        1 aaactgatca ccgcaatgat cctcccgcca tttaatatta ttttgctgtg gttgttagca
       61 ttaatcctct tttctttagg ctataaatac gctgcttact tctttgcctt gttaggtatt
      121 ctgattttat atatcttcag cattccctat acttcacaga aattagggga ttcattagtc
      181 gaagcagagc catttaccat agagaaatat aaaaatgctc aagcgattgt ggtgctaggt
      241 ggcgggctac gagacagcca agagttattc ggcaaactgg caattaccgg tattccgctg
      301 gaaagaatgc gttatgccgc ttatctgcat aaagaaaccg gcttaccgat tttagcgacc
      361 ggaagcagcc caaatgggac ttctgaggca aaggttatgg cagatgaatt taaaatgttc
      421 ttcaatattg agaccaaatg gctggaaaat aaggcgaaaa ccaccaaaga aaatgcgatt
      481 ttcagccgtg agattttaga aaaagagaat attcataaaa tcattcttgt taccaaccaa
      541 tggcatatga aacgggctga aatgctgttt agccaacaag gatttgaggt gatgtctgcc
      601 agcgtcggtt ctggcgatac gcccgatcat tacaaactca actttatgca ctttattccg
      661 caagccggtg caatgaatag caatatgctg gcattaaaag agtggattgg ttattggaaa
      721 gagaaataac cgaagataaa tagcagcaag gcaggttttc acctgccttc tttttgcaaa
      781 aaatcagtca aaaacaaccg cttgtctaac taatctcttg tgggtcaatg tcgatattca
      841 gcctgatatt attcggaatg tttaaacttt ccctttcttg atcaaaggca tcgagtagct
      901 gttgtaatgc tcctcggaga gggtgttgaa tcagcaataa ccagcggtaa tgtcctgctt
      961 tcttcgccat tggggctgaa aatggtggag ggagctggaa gccttgcccg ttttgttgct
     1021 ggattttttg gctaaaataa tctgctaatt gctcaagcag ctgtaaggtt tgttgattat
     1081 ctttgccggt cgctttgaac aaaacttgcg aggaaaatgg tggcaaactc attaattgcc
     1141 tcatttttaa cgcctcatca gcaaaagctc ggtagccttg ttccagcaag gttttaagca
     1201 gtgggtgatc agggtaatgg gtttgtagca ccacttcgcc ttttttctcc gctcgccctg
     1261 ctctgcctgc cacctgtatg taaagctgtg ctaatttttc ttccgcacga aaatcagttg
     1321 aaaataacgc tgaatccaca ttaacaatcg ccaccaacgt cacattcggg aaatgatgcc
     1381 cttttgccaa catttgcgta ccaatcaaaa tttggctttt gccctctcga atagcggtca
     1441 aatggttttc caatgcacct ttgcgagcgg tagaatctct gtctatacga gtcacttggt
     1501 aatgagggaa ttgctgattt agcacctgct caagttgttc tgtgccgaca ccggtagtta
     1561 ttaaatgggt tgaaccacaa tgtccgcatt ggcggggaat taccctttgg gtggcacaat
     1621 gatggcaacg caacactttt tgcttttggt ggtaagtaaa aggcttatta cacgcctcgc
     1681 actcacaaac ccaaccgcat tcgtgacaaa gtaataccgg ggcaaagcca cggcgattta
     1741 aaaacagcat cacttgattg ccttgctcta actgggtttt catcattgaa agcaagcgct
     1801 ccgacaaacc ggcagttacc cgttgcgttt ttaaatcaat aatctgctga ttggcaagtt
     1861 gtgcattgcc ggcccgagcg gttagtgtca gttcgataaa tttcccgttt tgcacattgt
     1921 gcaaactttc aagacttggc gttgccgagc ccagcacaat cggaatattt gcatttttcg
     1981 ccctaaccac cgccaaatcc cgtgcgtggt aacgccaacc gtcttgttgc ttaaacgagc
     2041 tatcgtgttc ttcatccaag ataatcacgc ccaatttttg aaactgggta aataaagcag
     2101 agcgagtgcc gattacaatc gcactttctc catttttcgc ccgcaaccac gcatttaacc
     2161 gctcagtttc gttcatattc gagtgcaata cgtcaatttc aacgttaaag cgagctttaa
     2221 agcgttgtac cgtttgcggt gttagcccga tttcaggcac aagcagcagc acttgttcct
     2281 ctcgcttaag caccgtttca attaactgca aatacacttc ggttttgccc gagcctgtta
     2341 cgccattgag taaaaagctc gcaaaagtgg tttgtgcatt taaacggctg accactaagg
     2401 tttgttgttt atttaagatc agccgattgt tagtatttac taacggaaat tcacctagtg
     2461 agtcttgcca cgcttgtgcc ataaacggca catcaatttt ctcgatatac cctttttcga
     2521 gtaagccttt ccaagcagat gggctacaag cggtcggttt ttcaaaatat tttgcacatt
     2581 cggcaagctc ggttaaccgt tccacctgct ttttggcttt tttattttca ccgataatca
     2641 gagatttccg cccttgctcc gtcaccacaa aataattcgg ttgaactcgc tcagtggaat
     2701 cgccattgcg taattttatc ggtaaggctc cggttagcac ttcaccaatc ggggcgtggt
     2761 aataacgagc cgcccagttc agcagttgcc acatttctga gtcaaaaatc gggctagaat
     2821 ccaacgctgc tttaatcggt ttgagttttt cggttggcac atcactagtt tgcgggaaat
     2881 cgaccacaat gccgattttg gtctggcgac caaaaggcac aaccacccgc ccgccttggc
     2941 aggcattgaa tgccggcggt agcaaataat caaaataacg atgtaacggc acattcagtg
     3001 ccactctgat taatctcact tctctaaacc aattttgatt gcaagcccga taaatatcga
     3061 gccgcagatt ttatttaacc aatttccaaa ggtttgatta tgccgtaaaa aacgcccaac
     3121 aggtgcagcg gctaagacta aaatacttac ccaaataatg ctggtggata aaaacgttag
     3181 ccccaacagc ataaaaggta ttttccaaat tactggatac aaactgcggc aataacgcca
     3241 agaaaaagag caatactttc gggtttaata gattggtaat caacccttga cggaatatct
     3301 tccatagagg aagtttgtct agattgattt tctctaacac caacggctct cgccacattt
     3361 taatgccgag atagcagaga taaattgccc ctgcgtattt cagaaaatta aataaagtgg
     3421 gcgaatgtgc cacaatgcct gcaagcccga gtgaaaccgc aagaatatgg cataaaatgc
     3481 ctgctagaat gccgaatgaa gacattaagc ccgacttttt cccctcagca atagtgcgag
     3541 ttaaaatata gatagtatcc gaacccggtg taagatttag cagaatacaa gcggtcaaaa
     3601 atccccaata atttacaata tcactcatta ttcacactca atttacgttt acgctgctga
     3661 cgaagagcaa acataccatc taatactaaa aatgaaatgg agatcaacaa gcaaatcatc
     3721 aacaagtaag attcatttgc taacctttcg ccaattaaga aagacaccac caacatcaat
     3781 agtggctcaa catagcctaa taaaccaagc aaattaaagg gtaaaatcgt gctggctagc
     3841 gtatagctga taagtgcagt gccactaatt aaacccagta atgcaataaa atagtaaata
     3901 ttgaggtttt gtgtttcgac atattgcata tcggtttgag agataaaata acaagctaca
     3961 gggagcaaat ataaaatttc taccacaaag ctatgtaaat ggcttaagcc aaactttcgg
     4021 cgaaggtaaa aatagattgg gtaacctgtg caaacaaata aacttgctat cgaaattttt
     4081 cccgtaaaca aaatattact cgctacacca atgaaagcaa ccacaatcgc caaccattta
     4141 ttcaaggaaa gcgtttcttt ataaaccact ttgccaaatg ccaccataat aatcggcatt
     4201 aatagatagc ccattgaaac ctcaatcgct tttccgctat tgggagccca taaaaacagc
     4261 cacatctgcc cgcccacaat agatgccgtg attaaaataa ccagcaataa ataaggctct
     4321 tgctttaggc gttgaagaaa tacccaaaac tcattttgtt ttttaaataa gtataaagca
     4381 aggaaaagaa aaggaatcgt aaccagcata cgaatgccga aaatgctcac acctgccagc
     4441 ggttttagaa agatcgccaa atagtacata cagccgaata acactgaggc tgaaagagat
     4501 aaaagaatgc cttttaacat agtgataaga aagtaattat tttttaattt ttcaaatctc
     4561 ccctacccct cttttctaaa gaggattgtc gagttctaaa taaatgaata atgttcggag
     4621 gtaggggcgg gtccagtgcc cgcccgcaaa ttaatatcgt tagcggacag gcacaggacc
     4681 tgtccctact gatattatag aacttacaac cctgctttta taaagtgggg taaagggaga
     4741 ttcgacttag tcttgacgat aggttttcaa gaaattcgca aggcgaccaa tcgcttcttc
     4801 aatttgatgt gcataaggca aagttaccac acggaagtga tccggttttt tccagttaaa
     4861 accggaacct tgcactaata gcactttttc ttgtcgcaac aaatcgtaga tgaatttttg
     4921 gtcatcttta atgccgtaca tttcggtatc aattttcggg aacatatata acgccccttt
     4981 cgcttttaca cagctaatac ccggaatttg taccaataat tcgtgcattt tatttcgttg
     5041 ttccagcaaa cgaccgcccg gcaacacaaa ttcattgata ctttgataac cacctaatgc
     5101 ggtttgaatg gcgtgttgca tcggcgtatt ggcacataaa cgcatagaag agagcatatc
     5161 caacccttca ataaaacctt tggcttgatg cttcggaccg ctcagcacca tccagccctg
     5221 acggaaaccg gcaacacgat acgccttgga taaaccgtta taagttacgg ttaatacgtc
     5281 cggtgcaaga gcagcaatat ggtgatgtac cgcaccatca tagacaattt tttcataaat
     5341 ttcgtcagca aaaataatta agttatgctg acgggcaagt tctgcaattt ccagcaaaat
     5401 ttgacggcta tacactgctc cggtcgggtt attcgggttg ataaccaaaa tccctttagt
     5461 gcgaggcgtg attttcgatt taatatcttc aagatcaggg aaccactcat tttcttcatc
     5521 gcacaggtaa tgcactgctt taccaccggc tagggttgaa gctgctgtcc ataacggata
     5581 atcaggcatt ggaattagaa tttcatcgcc atcatttaat aacgcctgca tcgacattgt
     5641 aatcagttca gatacgccgt tgccgatgta aacatcgttt acatccatac cacgcatacc
     5701 ttttgattga taatattgca caatcgcttt gcgggcagaa tacaaacctt ttgaatcaca
     5761 atagccttgt gcggttggaa gattacgaat cacatccact aaaatttcat cgggtgcttc
     5821 aaagccaaaa ggggctgggt taccgatatt taattttaaa attttatgcc cttcttcttc
     5881 taagcgaagt gcctctttat gaattggccc acggatatcg taacgaacct gagctaactt
     5941 atctgatttg ggaaaacgat ccattgtaaa cttcctataa tgtattatct aattgtagtc
     6001 tcatcccgct gttcaaaaaa tcggtaattc ggaatgaatt tgtaaacatt actctaattt
     6061 gggaggaaaa aaaagaggaa atacgttaaa atggagacct ttttttcaat cagctaaaaa
     6121 caagcggttt ctttttctga tttttttgca aatgcttatt tttcatcaat taaaacaaga
     6181 acttacaaaa agttactcaa aagtgggaag agagcaagga ttggtcgcca ttcaagcctc
     6241 tatgccgcta ttggaggcaa atattccgct actcggttgg ttgaaaacac aaaaaacaag
     6301 ctaccctcac ttttttctgc aatatcgtga tacagatcag accattgcca ccattggcac
     6361 acttcgccaa ttttatacgc tagaagctgc acaatctttt attcaacaac atcatttccc
     6421 gttaatcggc gggcttcaat ttgaaggcaa tacgcaattt attttgcctc aattttcatt
     6481 ggtaaaaaat cagcaaaatt taaccgcttg cttctatttt gatagtgata actttgaaca
     6541 acaagcggtt atttttgaac aatttcttgc aaatttcagc caaattaccg aattaggact
     6601 aacggataat ccgctcgttt caatgagtgc caccagccat tttgagcaat ggaaagccaa
     6661 tattgaaaaa gcgattgttt ctattgagca gggcgatttt caaaaagtgg tattggcaaa
     6721 tgcgatgaga cttcattttg agcaacccat ttgtgcttac agcttgctgg cacaaagcca
     6781 aaaaacaaat ttaggttgtt accacttttt atgggcggaa agtgctgatg ttgcctttct
     6841 tggctcaacc cctgagcggc tatatcaacg ccaagggcta cacttttcta ccgaagccct
     6901 cgccggcacc gctgcggtta ccaccagtga agtgcaaacc gaactcaatg cacaatggct
     6961 gttaagtgat cccaaaaata tctatgaaaa tcaattagtg gttgatgaca ttgaaaacca
     7021 actcaaagat tgcgtgaccg aatttgcagt atccgaggca aaaattaagc gtttgcataa
     7081 tgtgcaacac ctacgccgcc aaattcaagc aacgctcaaa ccggacacct cagattcaga
     7141 ttgcttaagc aaaatccacc caactgcggc agttgcaggg cttccccgcc aaaaagcaaa
     7201 gggttttatt accgaaaatg agccttttac ccgccattgg tatgcaggta cattaggcgt
     7261 aatgggcaac gaggaagccg aattttgcgt aacactccgt tctgcattaa tttcacaaaa
     7321 ttcaattacg ctttatgccg gtgccggcat tgtgtcaggc tcagatccac aatcggaatg
     7381 gcaagaaatt aaacgtaagg cacaaggaat ggcaaaactg ttggatgttg atttagtaac
     7441 aaaaggggaa ctgtaagcta ggc
//
LOCUS       DANLXF010000114         7427 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.114, whole genome shotgun sequence.
ACCESSION   DANLXF010000114 DANLXF010000000
VERSION     DANLXF010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7427
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.114"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(7..825)
                     /gene="cysQ"
                     /locus_tag="PXD66_001570"
     CDS             complement(7..825)
                     /gene="cysQ"
                     /locus_tag="PXD66_001570"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HDL6319857.1"
                     /translation="MQTLTQPLLENIINIAKAAGEQLKQLYARSVAIKIKADCTPVTE
                     ADLIISQFLTERLKQLTPDIPVLSEEFCDIPFAERQQWQEYWIIDPIDGTQQFINRTG
                     QFSVVIALVQKREGRNQPVLGVIHAPILGKTYFSMEGSGSFLQENGEIRPLVKPKRAE
                     NALLIVTVGSDLHAEQAKNAISAPQIECVKYGSGSLKAGLVAEGKADCYIRFGDTGEW
                     DTAVAEVLLSEMGGKIFDFNFSPLTYNQRESLINPYFVMVADGSIDWDKIFLFK"
     gene            complement(839..1381)
                     /gene="nudE"
                     /locus_tag="PXD66_001571"
     CDS             complement(839..1381)
                     /gene="nudE"
                     /locus_tag="PXD66_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HDL6319858.1"
                     /translation="MIKQLPEILNIKTVAKSRIFEVQAVDLRFSNGEERTYERLTPNR
                     RSSVMVIPIQNNELIFVKEYAVGSERYELGFPKGIVDAGEEPIESANRELQEEIGFGA
                     KKLDFLRSLYSSPSHMYGLMHLFLAQDLYPSKLEGDEPEPLEIVRYPLDKIDQLLADP
                     DFAEARNLSALFMLREYLKK"
     gene            1469..1885
                     /gene="hslR"
                     /locus_tag="PXD66_001572"
     CDS             1469..1885
                     /gene="hslR"
                     /locus_tag="PXD66_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HDL6319859.1"
                     /translation="MKNKHEKEQNDAEVRLDKWLWAARFYKTRSIAKAMIEGGKVHYN
                     GQRAKVSKAVEIGAIIKLRQGNEEKEVEVLALSDQRRGAPEAQLLYSETEKSIKHREA
                     MAFARKANALSMPHPDRRPNKKERRDLIKFKEQEFS"
     gene            2129..3022
                     /gene="hslO"
                     /locus_tag="PXD66_001573"
     CDS             2129..3022
                     /gene="hslO"
                     /locus_tag="PXD66_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDL6319860.1"
                     /translation="MNYTKDNDKLYRYLFKDRAVRGEWVRLNNTFTETLNTHHYPKAV
                     QNLLGEMLVATSLLTATMKFNGNITVQIQGDSPLKLAVVNGNDQQQLRALARVQADIA
                     DTASLAEMIGNGVLVISIIPTDGERYQGVIALDKPTIRECLEDYFARSEQLQTHLVIR
                     TGEFEGKAVAGGMLLQIMPDGTGSRDDFEHLATLTETVKDEELFGLEAEELLYRLYHE
                     EQVEVYPPQDTEFKCGCSRERSGSALLLVPAEEIEEMLEEKQGVIDMQCECCGTQYFF
                     DKAAIEGFKEETEKLSKLGLN"
     gene            complement(3096..3734)
                     /locus_tag="PXD66_001574"
     CDS             complement(3096..3734)
                     /locus_tag="PXD66_001574"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015431925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDL6319861.1"
                     /translation="MQNKIDWQKVDGLLPVIVQNAQTCEVLMLGYMNQQALEKTLAEK
                     RVTFYSRTKQRLWTKGETSGNFLNVVDMSLDCDNDTLLILANPIGETCHTGAESCFHQ
                     FEQEQPDWIFFSKLERMIAERKNADPESSYTAKLYAKGTPKIVQKVGEEAVESVIAAM
                     KNDREELVGEVADLMYHLTVLLHNADLSWAEINAKLKERHKGIGLHPEGSNK"
     gene            complement(3762..4148)
                     /locus_tag="PXD66_001575"
     CDS             complement(3762..4148)
                     /locus_tag="PXD66_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL6319862.1"
                     /translation="MIKITALDHLVLTVADMQKTIDFYTTILGMQEITFGDNRKALLF
                     GTQKINLHQKGAEILPNAQNADCGTADLCLLTVTPLQEVIYVLQRHQVEILEGGIVPR
                     TGAVGKIESVYCRDPDGNLIEISLYV"
     gene            complement(4145..4918)
                     /gene="hisF"
                     /locus_tag="PXD66_001576"
     CDS             complement(4145..4918)
                     /gene="hisF"
                     /locus_tag="PXD66_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDL6319863.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAQRYAQEGA
                     DELVFYDITASSDSRTVDKSWVERVAQVIDIPFCVAGGIKTIADAEQIFAFGADKISI
                     NSPALANPELITELAKRFGVQAVVVGIDSWFEAETGKYWVNQYTGDEKRTRQTEWQLL
                     DWVEEVQKRGAGEIVLNMMNQDGVRNGYDLVQLKKVREVCQVPLIASGGAGEMVHFRD
                     AFIEANVDGALAASVFHKQIINIGELKEYLAKEGVEVRR"
     gene            complement(4900..5649)
                     /gene="hisA"
                     /locus_tag="PXD66_001577"
     CDS             complement(4900..5649)
                     /gene="hisA"
                     /locus_tag="PXD66_001577"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006995165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDL6319864.1"
                     /translation="MQKSIIIPALDLIDGQVVRLHQGDYAKQTTYADDPIAQFADYLA
                     QGAEQLHLVDLTGAKNPAQRQTALIGKIIRETHCKIQVGGGIRTEQDVADLLAVGANR
                     VVIGSTAVKEREMVKGWFKKYGAEKFVLALDVNIDAIGQKIIAMSGWQEASGVSLEEL
                     IEDYQTVGLQHVLCTDISRDGTLAGSNVDLYREICAKYPEIQFQSSGGIGSLADIKAL
                     CGTGVAGVIVGRALLEGKFNVAEAIECWQNG"
     gene            complement(5819..6664)
                     /locus_tag="PXD66_001578"
     CDS             complement(5819..6664)
                     /locus_tag="PXD66_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor protein"
                     /protein_id="HDL6319865.1"
                     /translation="MNEIKLFENSQIRSVWDSEKEEWFFSVVDIIEVLTQSSNPRRYW
                     SDLKRKIKDEEGGIELYEKIVQLKLKAPDGKMRETDATDLQGIFRIIQSVPSPKAEPL
                     KMWLAEVGKERIDEIIDPELTIDRALATYLKKGYSREWINQRLQAIQVRKELTDTWQD
                     HGVQVGREFAILTNEITQAWSGMTTRQYKDFKGLKKESLRDNMTTTELVLNMLAEAAT
                     KDIAQISHPQGLEENKTVAKRGGNVAKVARESLEAETGKPVITQQNAIDFAAVIEQVA
                     KQVKK"
     gene            complement(6763..7356)
                     /gene="hisH"
                     /locus_tag="PXD66_001579"
     CDS             complement(6763..7356)
                     /gene="hisH"
                     /locus_tag="PXD66_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015431920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDL6319866.1"
                     /translation="MTNLTIIDTGCANLSSVKFAFDRLGIRAEISRDLNKIQSADKLL
                     LPGVGTAAAAMAILHDRRLIDTIRNATQPMLGICLGMQLMTDFSHEGDVATLNLMSGK
                     TERLPECGLPLPHMGWNKVQYQADHPLFHDIEQGNHFYFVHSYGVLPNENTIATCDYG
                     VPFSAAIASQNFYGVQFHPERSGEAGAKLLRNFVENV"
BASE COUNT         1951 a         1844 c         1553 g         2079 t
ORIGIN      
        1 aactacctat ttgaataaaa atattttatc ccaatctatc gagccgtcag ccaccatcac
       61 aaaataagga ttaatcaaac tttctcgctg attataagtc agcggcgaaa aattaaagtc
      121 gaaaattttg ccgcccattt cacttaacag cacttctgcc acagcagtat cccattcgcc
      181 ggtgtcacca aaacggatat aacaatcggc tttcccttct gccactaaac cggcttttag
      241 actgcccgaa ccatatttta cacactcaat ttgtggggct gaaatggcat ttttagcttg
      301 ttctgcatgt aaatcgctgc ctacggtaac aatcaaaagg gcattctccg cacgtttcgg
      361 tttgacaagc ggtcgaattt ctccattttc ttgcaaaaag ctaccgctac cttccatact
      421 aaaataggtt tttcctaata tcggagcatg aataacgccc agcaccggct gatttctacc
      481 ctcacgtttt tgcactaacg caatcaccac tgaaaattga ccggtgcggt taataaactg
      541 ttgtgtgccg tcaatcgggt cgataatcca atattcttgc cattgttggc gttcggcaaa
      601 cggaatatcg caaaactctt ccgataatac cggaatatct ggtgtgagtt gcttcaagcg
      661 ttcagttaaa aattggctga taatcagatc cgcttccgtc accggtgtgc aatcggcttt
      721 gattttaatt gccaccgagc gggcataaag ctgctttaac tgctcaccgg ctgctttggc
      781 aatattgata atattctcga gtaggggttg agttagcgtt tgcataatag ctccttgcct
      841 atttttttaa gtattctcgt agcataaata gagcggacaa attccgtgct tcggcaaaat
      901 caggatctgc caataattga tcaattttat ccaacggata acgcacaatt tccagcggtt
      961 caggctcatc gccttccaat tttgatgggt acagatcttg tgccaaaaac aggtgcatta
     1021 agccgtacat atggcttggc gaactgtaaa gcgaccgcag aaaatctaat tttttcgccc
     1081 caaaaccaat ttcttcttgt aattcacggt tcgcactctc aatcggctct tcacctgcat
     1141 ccacaatgcc tttcggaaaa cccagctcgt aacgctccga gccgaccgca tactctttca
     1201 caaaaatcag ctcgttgttt tgaatcggaa tcaccattac cgaagaacgg cgattcggcg
     1261 taagtcgttc gtaagttcgc tcttcgccat tagaaaagcg taaatcgacc gcttgcacct
     1321 caaaaatgcg agatttcgct actgttttaa tgttcaaaat ttcaggaagt tgttttatca
     1381 ttttatctca cctttttgag ttattttagt tgaaaaatct cactttcgct ctaatatttc
     1441 gactattgtt ttgattttaa ggcagaaaat gaaaaataaa cacgaaaaag aacaaaacga
     1501 cgccgaagtt cggctagata aatggctttg ggcggctcgt ttttataaaa cccgttcgat
     1561 tgccaaagca atgattgaag ggggtaaagt ccattataat gggcaaagag ctaaagttag
     1621 caaagcggtg gagatcggtg ccatcatcaa acttcgccaa ggcaatgaag aaaaagaggt
     1681 ggaagtgtta gccctcagtg accaacgccg aggggctccg gaagcccaac tgctctacag
     1741 tgaaaccgaa aaaagcatta aacaccgtga ggcaatggca tttgcccgca aagcgaatgc
     1801 tctttcaatg ccacaccctg atcgccgtcc aaacaaaaaa gaacggcggg atttaatcaa
     1861 attcaaagag caagaattta gctaagccct tgcaatcaga aaaataatcc ctatattgaa
     1921 tatcctaagt aggggcgaaa aatttttcgc ccctatataa ttatttcggg cgaacattga
     1981 ttcgccccta cagcgttcga tttgcacatt tttagccaca aaatgcaatg cattttgaac
     2041 gttggctttg ccaacggctc cgtaggagcg aacaaaatta gaaataattt tgtgagtaat
     2101 aatcgaccgc ttgcaacgag gaatcaaaat gaattacacc aaagacaatg acaaactcta
     2161 ccgctattta ttcaaagacc gagcggtacg gggcgaatgg gtgcgtttaa acaacacctt
     2221 taccgagacc ttaaacaccc atcactaccc taaagcagta caaaatttac tcggagaaat
     2281 gttagtcgca accagtttgc tgactgcaac aatgaaattt aacggcaaca ttactgtgca
     2341 gattcaaggt gacagcccgt taaaattggc ggttgtgaac ggtaacgacc aacagcaact
     2401 ccgtgcttta gcccgtgttc aggcagatat tgcagacaca gcctcgttag cggaaatgat
     2461 cggcaatggt gtattagtga tttctattat cccaaccgat ggtgaacgtt atcaaggggt
     2521 gattgctctc gataaaccaa ccattcgtga atgtttagaa gattacttcg cccgctccga
     2581 gcaattacaa acccacctcg ttatccgcac aggcgaattt gaaggcaaag cagtggcagg
     2641 cggtatgttg ctacaaatta tgccggacgg cacaggctca agagatgatt ttgagcattt
     2701 agccacgctc actgaaaccg taaaagatga agagttattt ggtttagagg cagaagaatt
     2761 gctctatcgc ctctaccacg aagagcaagt agaagtttac ccaccacaag atactgaatt
     2821 taaatgtggc tgctcaagag agcgttcagg ctccgcatta ttactggtgc cggcagaaga
     2881 aatcgaagaa atgttggagg aaaaacaagg cgtgatcgat atgcaatgtg aatgctgcgg
     2941 gacacaatat ttctttgaca aagcggcgat tgaaggcttt aaggaagaaa ctgaaaaatt
     3001 aagtaagtta gggttaaatt aagctaaaca agcggtcaag tttcttgtct tttttgcaat
     3061 ttgtaaaaaa acggtgaaat ttgaccgctt gcattttact tatttgagcc ttctgggtgt
     3121 aacccaatcc ctttgtggcg ttcttttagt ttagcattga tctctgccca cgagagatcg
     3181 gcattgtgca ataacaccgt taagtgatac atcagatcag ccacttcacc gaccaactct
     3241 tcccgatcat ttttcattgc cgcaatcacg ctttctactg cctcttcccc tactttttgg
     3301 acgatttttg gcgtgccttt ggcgtaaagt ttagcggtat aagaactttc cggatcagca
     3361 tttttacgtt cggctatcat tcgttctaat ttgctgaaaa agatccaatc tggttgttct
     3421 tgttcaaact gatgaaaaca gctctccgcc cccgtatggc aggtttcgcc gatagggtta
     3481 gcgaggatta acagggtgtc gttatcacaa tccaaactca tatccaccac gtttaggaaa
     3541 ttgcccgagg tttcgccttt cgtccacagc cgttgtttag tacgggagta aaaagtcact
     3601 cgcttttcag ccagcgtttt ttcgagggct tgttggttca tatatcctaa catcagtact
     3661 tcgcaggttt gggcgttttg gacgataacg ggcagtaagc cgtccacttt ttgccagtct
     3721 attttatttt gcattttttc ttcctttttt tgaccgcttg tttagacata taggcttatt
     3781 tcaatcaaat taccatcagg gtcacggcaa taaaccgact caatttttcc cactgccccc
     3841 gtacgaggca caatcccacc ttccaaaatc tccacctgat gtcgctgtaa cacatagata
     3901 acctcctgaa gtggagttac cgttaagagg cataaatctg ccgtgccaca atcggcattt
     3961 tgggcattgg gtaggatttc tgcacctttt tggtggaggt tgattttctg tgtgccaaac
     4021 aaaagcgctt tgcgattatc gccaaaggtg atttcttgca tacccaaaat ggtcgtataa
     4081 aaatcaatgg ttttttgcat atcggcaacg gttaaaacca gatgatctaa ggcagtaatc
     4141 ttaatcatct acgcacctca accccttcct tcgccaaata ttccttcaac tccccaatat
     4201 tgataatttg tttatgaaac acactcgctg ccaacgcacc atccacattt gcctcaataa
     4261 aagcatcacg gaaatgcacc atttcgcctg caccgcctga ggcgatgagt gggacttggc
     4321 aaacttcacg cacttttttc agttgcacca aatcgtagcc gttgcgtacg ccgtcttggt
     4381 tcatcatatt cagcacgatt tcgcctgctc ctcgcttttg cacttcctcc acccaatcca
     4441 acaattgcca ttcggtttgg cgggtgcgtt tttcatcgcc ggtatattga ttgacccaat
     4501 atttgccggt ttctgcctcg aaccagctat caataccaac caccaccgcc tgcacgccaa
     4561 agcgctttgc cagttcggta atcagctcgg ggttggcaag ggctggggaa ttgatggaga
     4621 ttttgtccgc tccgaaagcg aaaatctgct ccgcatcggc aatggttttg attccgcctg
     4681 ccacacagaa cggaatatca atcacctgtg ccacacgttc cacccagctt ttatccaccg
     4741 ttctgctgtc acttgaggcg gtaatatcgt aaaacaccaa ctcatccgcc ccctcttggg
     4801 cataacgctg ggcgagtggc acaatgtcgc cgatgatttc gtggttgcgg aactgcacgc
     4861 ctttcacaac ttgcccgtca cgcacgtcta aacaaggaat tatccgtttt gccaacattc
     4921 gattgcctcc gctacattaa atttaccttc caacaacgca cgaccgacaa tcacgccagc
     4981 cacgcccgta ccacaaagcg ctttgatgtc ggcaagcgag ccgataccgc ctgaggactg
     5041 aaattgaatc tctgggtatt tggcacagat ttctcgatag agatctacat ttgaacctgc
     5101 cagcgtgccg tctcgggaaa tgtcggtgca taaaacgtgt tgcaagccca cggtttgata
     5161 atcttcaatc aactcctcga gcgacacacc acttgcctct tgccagccgc tcatcgcaat
     5221 gattttttga ccgattgcat caatgttcac atccagtgcc agcacaaatt tctccgcacc
     5281 gtactttttg aaccagcctt taaccatttc acgctctttt accgcagtcg aaccaatcac
     5341 cacacggttt gctccaacag caagtaaatc cgccacatcc tgctcggtgc ggataccgcc
     5401 gcccacttga attttgcagt gggtttcacg aataattttg ccgataagtg cggtctgtct
     5461 ttgagccgga tttttcgccc ctgtaagatc gaccaaatgt aactgctccg ccccttgtgc
     5521 caagtagtcg gcaaattggg caatcgggtc atcggcataa gtcgtctgtt tcgcataatc
     5581 gccctgatgt aggcgaacta cttgcccatc gataaggtcg agggcgggga taatgatgga
     5641 tttttgcata ttattgttcc tgtgtgccta gcacgagccg tgctaggctg aataatccca
     5701 gtcgcgctgc gactgcctct ctaagctacg gagtagcttg gtttatctag ccccatacgg
     5761 ctcgtatggg gtacaagcgg tcaaattctt ccaaaaactt gcaaaaaacc gaccgcactt
     5821 atttcttcac ctgtttcgcc acttgttcaa tcaccgcagc gaaatcaatc gcattttgtt
     5881 gcgtaatcac cggtttgccg gtctctgcct ccaaactttc ccgagccact tttgcaacat
     5941 ttccgccccg tttggcaacg gttttatttt cttccaagcc ctgtgggtgg ctgatttgag
     6001 cgatgtcttt ggtggcagcc tcagcaagca tattcagcac caactcggtg gtcgtcatat
     6061 tgtctcgcag actttccttt ttcagccctt tgaaatcctt atattggcga gtagtcatac
     6121 cgctccacgc ctgtgtgatt tcattggtga gaatcgcaaa ttctctgccc acttgcacac
     6181 cgtgatcttg ccaagtatcc gtcagttctt ttcgcacctg aatggcttgc aagcgttggt
     6241 taatccactc acgggaatat ccctttttca agtaagtagc caaagctcgg tcaatggtca
     6301 attcaggatc gataatctca tcaatccgct ctttgcctac ctctgccagc cacattttca
     6361 acggctcggc tttcgggctt ggcacagact gaataatacg gaaaatgcct tgtaaatccg
     6421 tggcatcggt ttcccgcatt tttccgtctg gggcttttaa tttcaactgt acgatttttt
     6481 cgtacagttc aattccgccc tcttcgtcct tgattttccg ctttaaatca ctccaataac
     6541 ggcgtggatt gctactttgc gtcagcacct cgataatgtc caccaccgag aaaaaccact
     6601 cttctttttc gctatcccac accgagcgga tttggctgtt ttcaaatagt ttaatttcgt
     6661 tcatttctca ccttctttca ctggttaaat taacagtaat tttgtcgaaa taaagcgaga
     6721 aatcaagccc tattttcaaa cttttcgatc gagatcacaa aatcagacat tctccacaaa
     6781 attcctgagc aacttcgccc cagcctcgcc cgaacgctcg gggtggaact gtacgccgta
     6841 gaagttttgg ctggcaatgg cagcggaaaa cggcacgccg tagtcgcagg tggcgatggt
     6901 gttctcattc ggtagcacac cgtagctatg cacaaaatag aaatgattgc cttgctcaat
     6961 gtcgtgaaat agcggatggt cggcttggta ttgcaccttg ttccagccca tatgcggcaa
     7021 cggcaaaccg cattcaggca agcgttcggt tttgccactc attaagttca gtgttgccac
     7081 atcgccttcg tgggaaaagt ccgtcattaa ctgcattcct aagcagatgc caagcatcgg
     7141 ctgggtggcg ttgcgaatgg tgtcgataag tcggcgatcg tgcaagatcg ccatcgccgc
     7201 cgctgctgtt cccacaccgg ggagtagcaa tttatcggca gattggattt tgttgagatc
     7261 tcgcgagatc tcagcccgaa tgccaagacg gtcaaaagca aatttgaccg aagacaggtt
     7321 ggcacagcct gtgtcgatga tggtgaggtt ggtcatttag tttcctgagt taagtatttc
     7381 ttatagtgaa gtaaaattga ctcaatatat ctgtttcctt caaaagc
//
LOCUS       DANLXF010000115         7423 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.115, whole genome shotgun sequence.
ACCESSION   DANLXF010000115 DANLXF010000000
VERSION     DANLXF010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7423
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.115"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            49..1845
                     /locus_tag="PXD66_001580"
     CDS             49..1845
                     /locus_tag="PXD66_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate-specific subunit IIBC"
                     /protein_id="HDL6319867.1"
                     /translation="MDFLTEILLFLKDQVLNKAPFLLGIVACLGYILLKKDTSTIIKG
                     TIKTIVGFMIVQAGSGFLVTNFKPVIDGLAKYHQLTGAVIDPYTSMQSTIQTMGENYA
                     WVGYAVIGALFLNILLVVFRRLTGIRTIMLTGHIMFQQAGLVAVFYMIIGASMWETII
                     YTSVLMALYWGISSNIMYKPTQAVTGGAGFSIGHQQQIASWIAVKLAPKLGDKDDTVD
                     KMKLPNWLNIFHDSISATALVMTIFFGIILLSFGLDNLKELAGKTNQFMYILEMGLKF
                     AVAIQIIVTGVRMFVAELSEAFKGIAERLIPNSVLAIDCAAIYAFSPNAMVFGFMWGA
                     IGQFVAVGGLLAVGSPVLIIPGFIPMFFSNATIGVFANQFGGWKAVMKICFVMGIIEV
                     LGSAWVIQMLASQGTTFNGWMGMADWALFFPPILQGIISIPGFFFVIVAAAMVYMFFA
                     SKQLRAEEAEAAARGITLEQLDGYGLDEVKAEPKAEAVVQQAVENAENSANPIRILAV
                     CGSGQGSSMMMKMKIKGYLDKRGIPNIMDSCAVTDYKGKLSEVDIIVSSKHLANEIEV
                     PEGKAVLGVQNMLNPNSFGDELVELINQHNAK"
     gene            1925..2986
                     /locus_tag="PXD66_001581"
     CDS             1925..2986
                     /locus_tag="PXD66_001581"
                     /inference="COORDINATES: protein motif:HMM:NF012376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL6319868.1"
                     /translation="MKKILSTLFMLSAVSVAVAKEVNIKLLGTSDVHGRVVPWSYGAD
                     VEDKSGSFAQIATYVKEVRANHKNVLLFDIGDAIQDNQVEEFAKTLDKAKNNPVPKVL
                     NAMNYDIFILGNHEFNFGMPNLDAILKDINAKILTANFYHKDGKRYVEPTTIIERDGV
                     KLGIIGLTTPMSAIFEKDTGHLDAMKFTSPIEEAKAQIADLKAKGVDAIIVLAHMGIE
                     NENNIPDTGVADLVNAVDGIDVIIAGHMHKDVPSETIKNTLITEPHRYGTVVSEVDLA
                     FDVNDKGEVKLLSKTAKTVPVKKLASDPEIEKIYQPYHNELRALNNVKIGETAQDLIP
                     QGKITVYRLLSRKTPECPH"
     gene            2974..3438
                     /locus_tag="PXD66_001582"
     CDS             2974..3438
                     /locus_tag="PXD66_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HDL6319869.1"
                     /translation="MSSLIHDVQQHYSGADVVSFAFDYETVRLDKGDIKKKDIIYNYR
                     YAGGDVSMYELTGKQLKQYMEWSADYFDTIQAGDKDYRYNAVRGKSKYVTFDLFGGVS
                     YNIDLRNPSGSKIVDLKLANGSLITDDGKYKVGMNSYRFGQLTKKGGIWEGQ"
     gene            3469..3585
                     /locus_tag="PXD66_001583"
     CDS             3469..3585
                     /locus_tag="PXD66_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ser/Thr protein phosphatase"
                     /protein_id="HDL6319870.1"
                     /translation="MGAEKGTIQNMLIDYITNVKGGKIEGKSHNRWAIIGLN"
     gene            3601..4068
                     /locus_tag="PXD66_001584"
     CDS             3601..4068
                     /locus_tag="PXD66_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL6319871.1"
                     /translation="MNLKQSLIENNSILLNQSAANWEDAIKIGTDLLVKAGTIEPRYY
                     DNIVSKIKEMGPYIILAPGLAMPHARPEEGVIKTSFALVTFDTPIYFEGEDEPVHVML
                     TLAGSDSDQHMQGLVEITQVLDDPESDTGVDLDKIRRCKTAEEVYAVIDQALA"
     gene            4123..4803
                     /locus_tag="PXD66_001585"
     CDS             4123..4803
                     /locus_tag="PXD66_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDL6319872.1"
                     /translation="MSKPLLQIALDSLSLEKAVADAKQAENQVEIIECGTILAFAEGM
                     KAVSTLRVLHPNHIIVCDLKTTDGGAILAKMAFEAGADWLTVSAAAHPATKAACKKVA
                     DEFNAAHPELKVKKEIQIEIYGNWTVEKDAKEWVELGVTQAIYHRSRDAELAGKGWTT
                     EDVELMKQLSDLGIELSITGGIVPEDIHLFKEIKNAKAFIAGRALVGEKGKQTAEAIR
                     VEIDKYWN"
     gene            complement(4871..6013)
                     /locus_tag="PXD66_001586"
     CDS             complement(4871..6013)
                     /locus_tag="PXD66_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL6319873.1"
                     /translation="MKIVIAPDSFKESLTALEVAQAIQQGFARVFPEAEYQLVPMADG
                     GEGSVQSLVDATHGSLQKISVIAPLANEVEGFFGLSGDQKTAFIEMAAASGLHLVPME
                     QRNPLVTTSYGTGQLIKAALDLGVEKIILGIGGSATNDGGVGMLQALGAKFLNAEQRE
                     IGFGGEALSQIQQIDLSGLDSRLKQVEFEVACDVDNPLCGERGASAIFGPQKGATPEM
                     VKMLDHALLHFAETVKSQLGIEIAEKAGAGAAGGMGGGLLLLPNVTLRSGVQIILEAT
                     DLAEKLADADLVVTGEGRMDAQSIAGKTPIGVAKVAKQFGKPVIAIVGSLKEDYPVVY
                     QHGIDAVFPIIRQVSNLEAVLKSGKENLISTAENVARLWKISALQE"
     gene            complement(6023..7402)
                     /locus_tag="PXD66_001587"
     CDS             complement(6023..7402)
                     /locus_tag="PXD66_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005757929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDL6319874.1"
                     /translation="MSGIALILIFLVAIIIMIYMIAKWKVHPFLALMSISIALALVAG
                     LPLAKIPTIVGEGFSNTFKSIGIVIIFGAIIGTILEKTGAALKLADMVVKVVGQKRPE
                     LAMLIMGWVVGIPVFCDSGFVVLNPIREAIRKKILANPVGMAVALSAGLYASHVFIPP
                     TPGPIAAAGAVGLSHNLLLVIGMGFVVSIPVLIASYFFAKYVGKQVSVMEEAQADQVI
                     SQSYEDLIKQYGKLPSGFLSFAPIFMPIILMAIGSIAKIAGVSGDFGVFLQFVGNPII
                     ALAIGVIFAIFLLVSSGKMAEFDSLTNDTLKLVGPILFITAAGGVLGNVITQAGFVDY
                     IKQNAHIISTAGIFFPFLISAILKTAQGSSTVAIITTASIMGMFNAPDSLMNALGLTS
                     EMAAALTVMAIAAGSMCVSHANDSYYWVVTNFTKLTPQQGYRTQTTLTFIMGVVGMLT
                     VYLLSLILL"
BASE COUNT         2178 a         1725 c         1612 g         1908 t
ORIGIN      
        1 tctctggagt tttttagttc tattaatctc tcaacaaaga ggccccctat ggacttttta
       61 accgaaattc tccttttctt aaaggatcaa gtgctgaata aagcaccctt cttactaggt
      121 atcgtagctt gcttaggtta cattcttctt aaaaaagaca cttctacgat tattaaaggc
      181 acaattaaaa caattgtcgg ttttatgatt gtacaagccg gttcaggttt cttagtaacc
      241 aactttaagc cggttattga tggcttagcc aaatatcacc aacttaccgg tgcagtaatt
      301 gacccttaca ccagtatgca atcgaccatt caaaccatgg gcgagaacta cgcttgggtt
      361 ggttatgccg taattggggc gctgttcctc aatattctat tagtggtgtt ccgccgttta
      421 accggtatcc gtaccattat gctcaccggt cacattatgt tccaacaagc aggcttggtt
      481 gcggtgttct atatgatcat cggagcgtca atgtgggaga ccattatcta cacctcagtg
      541 ttgatggctc tttattgggg tatttcgtct aatattatgt ataaaccaac ccaagcggta
      601 acaggcggtg caggcttctc aattggtcac caacaacaaa ttgcttcttg gattgcagtc
      661 aaactcgcac caaaacttgg cgataaagac gatactgtag ataaaatgaa attgcctaac
      721 tggctcaata ttttccacga cagtatttct gcaaccgcat tagtaatgac catcttcttt
      781 ggaatcatct tactttcatt cggtttagat aacctcaaag aacttgctgg caaaaccaac
      841 cagtttatgt acattcttga aatgggcttg aaatttgcgg tggcaattca aatcattgta
      901 accggcgtac gtatgtttgt agctgagctt tcagaagcct tcaaaggtat tgctgaacgc
      961 ttaattccaa actcagtatt agcgattgac tgtgccgcta tctacgcttt ctcgccaaat
     1021 gcgatggtat tcggctttat gtggggggct atcggtcaat ttgtggcagt aggcggcttg
     1081 cttgcagtag gctcaccggt gcttattatt ccgggcttta tcccgatgtt cttctctaac
     1141 gcaaccattg gtgtgtttgc aaaccaattt ggtggttgga aagcagtaat gaaaatctgt
     1201 tttgtgatgg gtattattga agtgcttggc tcagcttggg ttattcaaat gcttgcttca
     1261 caaggcacaa cctttaacgg ttggatgggt atggctgact gggcattgtt cttcccacca
     1321 atcctacaag gtattatcag cattccgggc ttcttctttg taattgtggc agcagcaatg
     1381 gtttatatgt tcttcgcatc taaacaactt cgtgccgaag aagcagaagc ggcagctcgt
     1441 ggcattacac ttgaacaatt agacggctac ggcttagatg aagtaaaagc agaaccaaaa
     1501 gcggaggcag tagtacaaca agcggtcgaa aatgccgaaa attctgcaaa cccaatccgc
     1561 attctagcgg tgtgtggctc tggtcaaggc tcatcaatga tgatgaaaat gaagatcaaa
     1621 ggctatctcg acaaacgtgg tattccgaat attatggatt cctgtgcggt aactgattac
     1681 aaaggcaaat taagcgaggt tgatattatt gtctccagca aacatttagc caacgaaatc
     1741 gaagtaccgg aaggcaaagc ggtattaggt gtacaaaata tgctaaatcc aaactcattt
     1801 ggtgatgaat tagttgagct aatcaatcag cataatgcaa aataagtaag tttaggggtg
     1861 aataatcacc cctatctatg ctaaagattg taaaaactca cttcatcaaa cgaggaatta
     1921 tccaatgaaa aaaatcttat ccaccctgtt tatgctctcc gcggttagcg ttgccgttgc
     1981 caaagaggta aacattaaac tacttggtac ctcagacgtt cacggacgtg ttgtgccgtg
     2041 gagctacggg gcagacgtgg aagacaaatc cggctcattc gcccaaattg caacctatgt
     2101 caaagaggtg cgtgccaacc acaaaaatgt gttgctgttt gatattggcg atgcgattca
     2161 agacaaccaa gtcgaagagt tcgctaaaac cttagacaaa gcgaaaaaca atcccgtgcc
     2221 gaaagtgctg aatgcaatga attacgacat cttcattctc ggcaaccacg aatttaactt
     2281 cggaatgccg aatttagatg cgattttaaa agacatcaac gccaaaattc tcactgccaa
     2341 cttctatcac aaagacggca aacgctacgt tgagccaact accatcattg aacgtgatgg
     2401 cgtaaaactt gggattatcg gcttaaccac cccaatgtcg gctatttttg aaaaagacac
     2461 cggtcattta gacgcaatga agttcacttc gccgattgaa gaggcgaaag cccaaattgc
     2521 cgatctcaaa gccaaaggcg tggacgccat tattgtgctg gcacatatgg ggattgagaa
     2581 cgaaaacaac attcccgaca ccggcgtggc ggatttagtg aatgcagtgg acggtattga
     2641 tgtaattatc gccggccata tgcataaaga tgtgccgagt gaaactatca aaaataccct
     2701 gattaccgaa ccgcaccgct acggcacagt ggtttccgaa gttgatcttg cctttgatgt
     2761 gaatgacaaa ggcgaagtga aactactttc taaaaccgcc aaaaccgtac cggtgaagaa
     2821 actcgcaagc gatccggaaa tcgaaaaaat ttaccaaccg tatcacaatg aactgcgtgc
     2881 cttaaacaac gtcaaaatcg gtgaaactgc acaagattta atcccacaag gcaaaatcac
     2941 ggtgtatcga ttgctttctc gcaagacacc ggaatgtcct cattgatcca cgatgtgcaa
     3001 caacattaca gcggtgcaga cgtagtgagt tttgcatttg actacgaaac cgtgcgttta
     3061 gacaaaggcg acatcaagaa aaaagacatc atctacaact accgctatgc cggtggcgat
     3121 gtgagcatgt atgagctaac cggcaaacag cttaaacagt atatggaatg gtcagcggat
     3181 tacttcgaca ccattcaagc cggtgacaaa gactaccgct acaacgctgt gcggggtaaa
     3241 tctaaatacg ttaccttcga cctattcggc ggcgtaagct acaacatcga cctacgcaat
     3301 ccaagcggta gcaaaattgt cgatctaaaa ctggcaaacg gcagtctaat caccgatgac
     3361 ggcaaataca aagtgggaat gaactcctac cgctttggtc agctcaccaa aaaaggcggc
     3421 atctgggaag gacagtaaat ccccgtgatt tgggaatcca aagtcgccat gggggctgaa
     3481 aaaggcacaa tccaaaatat gctgattgat tacatcacca acgtcaaagg cggaaaaatc
     3541 gaaggcaaat cgcataaccg ctgggcgatt atcggcttaa attaacgaaa gggaaacact
     3601 atgaacttaa aacaatctct tattgaaaat aactctattt tgctcaatca atcagcggcg
     3661 aattgggaag acgcaatcaa aatcggcact gatttattag tcaaagccgg tacgattgaa
     3721 ccacgctatt acgataatat tgtaagcaaa atcaaagaga tggggcctta tattattctt
     3781 gcaccgggct tggcaatgcc tcacgcccgt ccggaagaag gtgtgattaa aacctctttc
     3841 gctttagtga cttttgacac cccgatttat tttgagggcg aagatgagcc ggttcacgtt
     3901 atgctaaccc ttgcagggag tgactctgat cagcatatgc aaggcttggt agaaatcacc
     3961 caagtgctag atgacccaga gagcgacaca ggtgttgatt tagataaaat ccgccgctgt
     4021 aaaacggctg aagaagtgta tgcagtaatc gatcaggcat tagcctaata ttctacaagc
     4081 ggttcaaaaa catcaatatt ttgcaaatag aggaatacaa ttatgtccaa accattatta
     4141 caaattgccc ttgattctct ttcactggaa aaagcggtgg cggatgcaaa acaagcagaa
     4201 aaccaagtcg aaattattga atgcggtact attcttgctt ttgctgaggg tatgaaagcc
     4261 gtttccacct tgcgtgtatt gcacccaaat cacattattg tgtgcgactt aaaaaccacc
     4321 gatggcggtg caattttagc taaaatggca tttgaagccg gtgccgactg gctcacggtt
     4381 tcggctgcgg ctcaccctgc aaccaaagcg gcttgcaaaa aagtggcgga cgaatttaac
     4441 gctgctcacc cggagctaaa agtgaaaaaa gagattcaaa ttgaaatcta cggcaactgg
     4501 acggtggaaa aagatgccaa agagtgggta gaattagggg taacccaagc gatttaccac
     4561 cgctcccgtg atgcggaact agcaggtaaa ggttggacaa cggaagatgt tgagttaatg
     4621 aaacagcttt ctgacttagg cattgaatta tctatcaccg gcggtatcgt accggaagat
     4681 attcacctgt tcaaagagat caaaaacgcc aaagccttta ttgcaggccg tgctttagtc
     4741 ggtgaaaaag gcaaacaaac tgccgaagcc attcgtgtgg agattgataa atactggaac
     4801 taatccggca ttgaaaccca ataaaaatgg cgttctctca accggaacgc cattttttat
     4861 ctcatttttt ctattcttgc aaggccgata ttttccacaa tctcgctaca ttttcggcag
     4921 ttgaaatcag gttctctttg cctgatttta acaccgcttc taaattgctg acctgtcgaa
     4981 taatcggaaa taccgcatca atgccgtgtt ggtacaccac agggtaatct tctttcaatg
     5041 agccaactat cgcaatcacc ggcttgccaa actgcttagc gactttcgcc accccaatcg
     5101 gggttttacc ggcgatagat tgagcgtcca ttcgcccttc ccctgtcacc accaaatccg
     5161 catcggcaag tttctcagca agatcggttg cctcaagaat aatttgcacg ccggaacgta
     5221 atgttacgtt aggcagtagc agtaaaccac cgcccattcc accggctgca ccggcaccgg
     5281 ctttctcggc aatctcaatg cctaattggg attttacggt ttcggcaaaa tgcaacaggg
     5341 cgtggtcgag cattttaacc atttccggtg tagctccttt ctgtggccca aaaattgccg
     5401 aagcccctcg ctcgccacac agcggattat cgacatcgca agcaacctca aattccactt
     5461 gctttaagcg tgaatccaaa ccgcttaaat caatctgctg aatttggctt aatgcctcgc
     5521 caccaaagcc gatttcccgc tgttcggcat ttagaaattt cgctcctaag gcttgtagca
     5581 ttccgacacc gccatcgtta gtcgcactgc cgccaatgcc gagaatgatt ttttccaccc
     5641 ctaaatctaa ggcggcttta attaactgcc ccgtgccgta gctggtggta accagcggat
     5701 ttcgttgctc cattggcact aaatgcaagc cacttgccgc cgccatttca ataaaggcgg
     5761 ttttttgatc ccccgacaag ccaaaaaagc cctccacctc atttgcaagc ggtgcaatta
     5821 cgctgatttt ttgcaaactg ccgtgagtgg catcaaccag cgactgtacc gagccttcgc
     5881 caccgtctgc catcggtacg agctgatact ccgcctcagg gaaaacacgg gcaaaaccct
     5941 gttgaattgc ttgagccact tctaatgcgg tcaaactctc tttaaatgaa tccggtgcaa
     6001 tcacaatttt catcgtcaat ccttagagta aaatcaatga aagcagataa accgtgagca
     6061 tacccaccac acccataatg aaagttaggg tagtttgcgt acgatagcct tgttgcggag
     6121 tcagtttagt aaagtttgtt accacccagt agtagctgtc attggcgtgg gaaacgcaca
     6181 ttgagccagc cgcaatcgcc attacggtta aggctgccgc catttcactg gttagcccta
     6241 aggcgttcat taatgagtcc ggtgcgttga acattcccat aatggaggcg gtggtgataa
     6301 ttgccaccgt tgagctgcct tgtgcggttt ttaaaattgc cgaaatcagg aacgggaaga
     6361 aaatccctgc tgtactgata atatgagcgt tttgcttgat gtaatccaca aagccggctt
     6421 gagtaatcac gttacctagc acaccaccgg cggcggtaat aaagagaatt gggccgacta
     6481 atttcagggt atcattggtt aagctgtcaa attccgccat tttaccgctt gaaaccagta
     6541 ggaaaatagc gaagatcacc ccaattgcta acgcaataat cgggttgccg acaaattgta
     6601 ggaatacgcc gaaatcgcca cttacaccgg caattttggc aatcgagccg atagccatta
     6661 agataatcgg cataaaaatc ggggcaaagc ttaaaaaccc acttggtaat ttgccgtatt
     6721 gtttgatcag atcttcatag ctttggctga ttacttgatc cgcttgagct tcttccatca
     6781 cgctgacttg tttgccaaca tatttggcaa agaagtaact ggcaatcaac acaggaatcg
     6841 aaaccacaaa gcccattccg atcacaagca ggagattatg gcttaaacca acagcaccgg
     6901 ctgctgcaat cgggcccggt gtcggaggaa taaagacgtg ggaggcatat aaaccggcac
     6961 ttaacgccac cgccattccc accggattgg caagaatttt tttgcgaata gcttcacgaa
     7021 tcgggtttaa caccacaaag ccgctatcac agaacactgg gataccaacc acccagccca
     7081 taatcaacat tgccagttca gggcgttttt gccccaccac ttttaccacc atatcggcta
     7141 atttgagggc tgcaccggtt ttttctaaaa tcgtgccgat aatcgcccca aaaataatca
     7201 cgataccgat acttttgaag gtattactaa aaccttcgcc cacaatagtt ggaattttgg
     7261 caagcggtaa acctgccact aatgcaaggg ctatcgaaat cgacattaag gctaagaacg
     7321 ggtgaacttt ccacttcgca atcatataaa tcattatgat gatagcgacc agaaagatca
     7381 gaataagtgc aatacctgac ataataagtc tcctcattgt aga
//
LOCUS       DANLXF010000116         7413 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.116, whole genome shotgun sequence.
ACCESSION   DANLXF010000116 DANLXF010000000
VERSION     DANLXF010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7413
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.116"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(33..1616)
                     /locus_tag="PXD66_001588"
     CDS             complement(33..1616)
                     /locus_tag="PXD66_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL6319875.1"
                     /translation="MKKQFEHNESRRGFMKLIAGVGAGMAFSGTLGTFTPKAFAAPAA
                     GSTIEAGIAYPISTGFDPLTSSGASSMAANLHIFEGLVDLHPATRKPYLALAAAEPEK
                     IDDVTYRITLRDGAKFHNGNPVTTEDVVYSFERVLDPAKASLFAQFIPFIDTVKKVDD
                     KVVEFKLKYPFALFKERLTIVKIVPKAVVEAGQAAFDANPVGTGPYKFVSATKDDRIV
                     FEAFADYNGGYPAQVEKMTWFLLSDDAARVTAQESGRVQAIESVPYLDAERLKRKGTV
                     ESVQSFGLLFLMFNCEKAPFNNPKVRQALHYGLDTQKLIDVVFLGNAKAATSYVQDTH
                     PDYVKANSQYDFDKAKAEALLAEAGVTELKFELLATDHSWVKECAPLILESWNSLKGV
                     KVSLKHLQSGALYGTHVDKGAYEVVIAPGDPSVFGNDLDLLLSWWYRGDVWPKRRFRW
                     SETAEYAEVQKLLDEAAKNPAASKEAWAKAINIIAEQVPLYPIIHRKLPTAWNDKALT
                     GFQPLPTTGMSFIGVGRAK"
     gene            1838..2995
                     /gene="hemW"
                     /locus_tag="PXD66_001589"
     CDS             1838..2995
                     /gene="hemW"
                     /locus_tag="PXD66_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HDL6319876.1"
                     /translation="MNLILPPLSLYIHIPWCVQKCPYCDFNSHAQKGLIPEQEYIEHL
                     LADLSQDLTAYRVAIGDRKLYSIFIGGGTPSLFSAEGIAYLLIEVEKRIAFEPNIEIT
                     LEANPGTAEAERFLGYAQGGVTRISMGIQSFEPEKLLKLGRIHDANEAKQAIKFAQNS
                     AKSGLQSFNIDLMHGLPNQSVKQALADLETGISLNPPHLSWYQLTIEPNTMFYYRKPT
                     LPDDDELWDIFEQGHQLLTNAGYEQYETSAYAKKGYQCRHNLNYWRFGDYLAIGCGAH
                     GKISYPTGEIYRFSKIKHPKGYMRGEYRYSQDLIELDDRPFEFFMNRFRLLEATPKQE
                     FEWFTGLDREVVRPTIDWALNQNYISETATHWQITQHGKLFLNELLEGFLE"
     gene            3138..3620
                     /locus_tag="PXD66_001590"
     CDS             3138..3620
                     /locus_tag="PXD66_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HDL6319877.1"
                     /translation="MIDKQIRNSTAEFLIFTQQNGAENITVRYEDNEIWLTQKLIAQL
                     FDCSTDNVSLHLKNIFNSRELDPNSVTEDFSVTAADVKNYRTKHYNFDAVISIGYRVN
                     SVRATQFRQWATKVLRNFAQKGYVIDRQRMENGTFLNEDYFEQLLAEIREIWLSERRS
                     "
     gene            3589..3786
                     /locus_tag="PXD66_001591"
     CDS             3589..3786
                     /locus_tag="PXD66_001591"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319878.1"
                     /translation="MKFGYLNGVLEGKGKMRFDEAKLHAESEFEKYRIVQDRLFESDF
                     DKVLKEMLRSENQYRPSFSTT"
     gene            3873..4340
                     /gene="bcp"
                     /locus_tag="PXD66_001592"
     CDS             3873..4340
                     /gene="bcp"
                     /locus_tag="PXD66_001592"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HDL6319879.1"
                     /translation="MNILQAGDKAPKFTLQNQADEPVSLSQFAGKKVLVYFYPKALTP
                     GCTTQACGLRDSKSELDELNVVVLGISTDLPKKLAQFVEKKALNFTLLSDPDHQVAEA
                     FGVWGEKKFMGKTYDGIHRISFLIDEQGGVEQVFDKFKTGEHHQMIVDYLRGR"
     gene            4340..4888
                     /gene="gmhB"
                     /locus_tag="PXD66_001593"
     CDS             4340..4888
                     /gene="gmhB"
                     /locus_tag="PXD66_001593"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HDL6319880.1"
                     /translation="MGKKAIFLDRDGTINIDHGYVHQIDDFQFIDGVGKALKQLQDKG
                     YLLVLVTNQSGIARGYFSEAQFMQLTEWMDWSLDEDYGVVLDGIYYCPHHPEGKGEYR
                     KDCDCRKPKAGMFTQAIQDLNIDPSQSYMVGDKLEDILAAEAAGVKTKVLVRTDKAVT
                     EEGQAKADLVLDSLVDLVKYIK"
     gene            4893..5732
                     /gene="queF"
                     /locus_tag="PXD66_001594"
     CDS             4893..5732
                     /gene="queF"
                     /locus_tag="PXD66_001594"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HDL6319881.1"
                     /translation="MNYNDKSLSALKLGQKTEYKSEYDASLLQPVPRKLNRDSLGITQ
                     EQPFNRGADVWTCYELSWLNQNGLPQVAIADVAIDFKSENLIESKSFKLYLNSFNQTK
                     FSSIEEVGKTIQADLARCASGQVSVKIYQLSHYTQQPIVDFAGDCIDEQDIQIDSYAF
                     STHYLENIAEDELVEETLVSHLLKSNCLITSQPDWGSVQIRYKGKKLNREKLLRYLVS
                     FREHNEFHEQCVERIFCDLMQFAEPEKLTVYARYTRRGGLDINPFRSNFEEVPENLRM
                     ARQ"
     gene            5924..7057
                     /locus_tag="PXD66_001595"
     CDS             5924..7057
                     /locus_tag="PXD66_001595"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HDL6319882.1"
                     /translation="MLIFMMNEYSPNYYIGVMSGTSLDGVDIALLDFAKNPPKMTACD
                     FFPMPEELRADISALLKTGETNLQKLGEIDHRLGLLYAETINAFLAKNQLKAEDIQAI
                     GCHGQTVWHSPDSEYPFTTQIGDMNLVAEQTGITTIADFRRKDMAVGGQGAPLVPAFH
                     QAIFSDPNRLTVVLNIGGISNISVLNPNSPTVGYDVGAGNTLMDSWIEQHQGKRYDKN
                     GEWARTGKLNTELLATLLDESFFAKAPPKSTGRELFNLTWLAKKLENLTACQPQDVQR
                     TLAEFTAQSIANELLRFESSLPRLLLVCGGGSRNPLLMERISALLPEWEVTTTTEFGL
                     DIDYVEAAAFAWLAYQRIHNLPSNMPSVTGAKKAVSLGVIYPA"
     gene            complement(7054..>7413)
                     /locus_tag="PXD66_001596"
     CDS             complement(7054..>7413)
                     /locus_tag="PXD66_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL6319883.1"
                     /translation="PELVSVSFFVNNLIAVLLQSDFSRKLNLTVHKLIYIAPILIILQ
                     IMPWFYALQWLSTITAFLYLVIFATITVYANTALSNMLVKLDNGKNQGLMFGSSKLIL
                     AITTLGVMNVLPYIFLI"
BASE COUNT         2257 a         1423 c         1602 g         2131 t
ORIGIN      
        1 tattaccgct tgcatttagc tagccgttac gactatttag cacgaccaac accgataaat
       61 gacatacctg ttgttggtaa tggttggaaa cctgttaatg ccttatcatt ccacgcagtt
      121 ggcaatttac ggtgaataat tgggtaaaga ggcacttgtt cagcgataat attgattgct
      181 ttcgcccagg cttctttaga agcagcagga tttttggccg cttcatcaag cagtttttgt
      241 acttctgcat attcagctgt ttcagaccag cggaaacgac gtttcggcca tacatcacca
      301 cgataccacc agcttaataa taggtctaag tcattaccga aaactgatgg gtcgccaggt
      361 gcaatcacaa cttcgtatgc acctttatca acgtgtgtgc catataatgc acctgattgt
      421 aagtgtttga gtgaaacttt cacgcctttt aagctgttcc acgattcaag aattaatggc
      481 gcacattctt tcacccaaga gtgatcggtt gccaacaatt caaattttaa ctctgtcaca
      541 cccgcttctg ctaataacgc ttcggctttc gctttatcga aatcatattg cgaatttgct
      601 ttcacataat ccgggtgagt atcttgcacg taagatgtag cagcttttgc attgcctaag
      661 aacaccacat caattaattt ttgggtatct aagccataat gtaacgcttg gcgaactttc
      721 gggttgttaa acggtgcttt ttcacagtta aacattaaga acagtaagcc gaatgattgt
      781 actgactcaa cggtaccttt acgttttaag cgttctgcat ctaagtaagg cacagactca
      841 attgcttgaa cacggccaga ttcttgagcg gttacacgag cggcatcatc agaaagtaag
      901 aaccaagtca ttttctcgac ttgtgccggg tagccaccgt tataatctgc aaatgcttcg
      961 aaaacaatac gatcatcttt agttgcggat acaaatttat atgggccagt ccccactgga
     1021 ttagcatcaa atgcagcttg accggcttct accactgctt ttggtacgat tttaacaatg
     1081 gttaggcgct ctttgaataa cgcaaatgga tatttcaatt tgaattcaac cactttgtca
     1141 tcaacttttt tcacagtgtc aataaatgga atgaattgtg cgaaaagtga tgctttagct
     1201 gggtctaata cacgttcaaa tgagtaaact acgtcttcag tggtaaccgg attaccattg
     1261 tggaatttag ccccgtcacg taaagtaatt cgataggtta catcatcaat tttttcaggc
     1321 tctgctgctg ccaatgctaa atatggtttg cgagtggctg gatgtaagtc cacgaggcct
     1381 tcaaaaatat ggaggtttgc cgccattgag gatgcaccgc ttgaggtaag cgggtcaaat
     1441 ccggttgata tgggataagc tataccggcc tcaatcgttg agccggcggc aggggctgca
     1501 aatgctttag gtgtaaatgt acctaaagtg ccactaaatg ccataccggc accgacacct
     1561 gcaattagtt tcataaaacc acggcgggat tcgttgtgtt caaactgttt tttcatctta
     1621 aagctccttt aagtttttat gataggggcg gtattatttg ctgaagtgaa tttatcaaaa
     1681 aatgcacttt tttgaattga atttatcata tcattaatgt caaagtggta aagcaagaat
     1741 tattgaattt gtgatctagc tcacgaaaat ctttgctttt cacttcacgt gcttttcgtt
     1801 attattcgcc aatttaatca taaatgaaaa attgacagtg aacctgattt taccgccttt
     1861 aagcctttat atccatatcc cttggtgcgt gcaaaaatgc ccttattgcg attttaactc
     1921 gcacgctcaa aaaggattaa ttcccgagca agaatatatc gaacatttac ttgcagattt
     1981 atcccaagat ttgaccgctt accgagtggc aataggtgat cgtaaattat attctatttt
     2041 tattggcggc ggcaccccga gcttattttc agcggaaggg atagcttatt tattaattga
     2101 agtagaaaaa cggattgcat tcgagccaaa tatcgaaatt acactggaag cgaacccggg
     2161 aaccgcagag gcagagcgat ttttaggtta cgcacaaggt ggcgtaaccc gcatttcaat
     2221 gggaatccaa agttttgagc cggaaaaatt attgaaatta ggacggattc acgatgcaaa
     2281 tgaagcaaaa caagcgatta aatttgccca aaattctgca aaatcgggct tgcaaagttt
     2341 caacattgat ttaatgcacg gcttaccaaa ccaatcggtt aaacaagcct tggcggattt
     2401 agaaacaggg atttcactca atccaccgca tttatcgtgg tatcagctca cgattgagcc
     2461 gaatacgatg ttctattatc gtaagccaac cttgcctgat gatgacgagt tatgggatat
     2521 tttcgagcaa gggcatcaac ttttaacgaa tgccggctat gagcagtatg aaacctcggc
     2581 ttatgccaaa aaaggctacc aatgcagaca caatttaaat tattggcgat ttggggatta
     2641 tttggcgatt ggttgtggcg ctcacggcaa aatttcatat ccgaccgggg aaatttatcg
     2701 ctttagcaaa atcaaacacc cgaaaggtta tatgcgaggt gaatatcgct acagccaaga
     2761 tttgattgaa ttagacgacc gcccgtttga gttttttatg aaccgcttta gattacttga
     2821 agcgacacca aaacaagaat ttgaatggtt tacagggtta gacagggaag ttgttcgccc
     2881 gacaatcgat tgggcgttaa accaaaacta tatttccgaa accgcaaccc attggcaaat
     2941 tacccaacac ggtaaattgt ttttaaatga attactggaa ggatttttgg aataatttgc
     3001 aaaaaaacga ctaaaaaaag accgcattgt aatgctaaat tacaggcgtt ttttgtgatc
     3061 tgcgtcgaaa aatcctcatt ttttactaga caaaaaaaca gtatttagta gcatagcctt
     3121 cacaataagg agagcttatg atcgataaac aaatccgtaa tagtaccgcc gaatttttga
     3181 tttttactca acaaaatggg gcagaaaata ttaccgttcg ctatgaagat aacgaaattt
     3241 ggcttacgca aaaattaatt gctcagcttt ttgattgctc aacagataat gtttctttgc
     3301 atttaaaaaa catttttaat agtagggaac tcgatccaaa ttcagttacc gaggatttct
     3361 cggtaactgc cgcagatgtc aaaaattatc gtaccaaaca ctacaacttt gatgccgtta
     3421 tttcgatagg ctatagggta aactcggtgc gggcgacaca atttcgccaa tgggcaacta
     3481 aagtattacg gaatttcgcc caaaaaggct atgtgattga ccgccaaaga atggaaaatg
     3541 gtacattttt aaatgaagat tattttgagc agttgctggc agaaattcgt gaaatttggt
     3601 tatctgaacg gcgttcttga gggcaaaggc aaaatgcgtt ttgatgaagc gaaattgcac
     3661 gcagaaagcg aatttgagaa ataccgcatt gttcaagaca gattatttga aagcgatttt
     3721 gacaaggtat tgaaagaaat gttgcgttcg gaaaatcaat accgtccatc attttcaaca
     3781 acataaaaca accgcatttt gcaaattttt tgccaagttt gaccgcttgc cgtgtataat
     3841 gcaagcggtt attttttatg gaaaaattac aaatgaatat cttacaagca ggcgataaag
     3901 cccctaaatt tacccttcag aatcaggctg acgagccggt ttctctctct caatttgccg
     3961 gcaaaaaagt actggtttat ttttatccga aagctcttac acccggttgc actacgcaag
     4021 cgtgcggact gcgtgacagc aaatcagaac ttgatgagct caatgtggtt gttctcggaa
     4081 ttagcaccga tttaccgaaa aaattagctc aatttgtaga gaaaaaagca cttaatttta
     4141 ccttattatc tgacccggat caccaagttg cagaagcctt tggtgtatgg ggcgaaaaga
     4201 aatttatggg aaaaacctat gacggcattc atcgcatcag ttttttaatt gatgagcagg
     4261 ggggggttga gcaagtgttt gataaattta aaaccggcga acatcaccaa atgatcgtgg
     4321 attatctacg gggtcgctaa tgggaaaaaa agccattttt ttagaccgtg acggcacaat
     4381 taatatcgat cacggctatg tgcatcaaat tgatgatttt caatttattg acggtgtcgg
     4441 caaggcgtta aaacagctac aagacaaagg ttatttattg gtgctggtaa ccaatcaatc
     4501 tggcattgcc cgaggctatt ttagcgaagc acaatttatg caactaaccg aatggatgga
     4561 ctggtcgctc gatgaagatt atggcgtggt actagacggt atttactact gcccacatca
     4621 ccccgaaggc aaaggcgaat accgtaaaga ttgtgattgc cgtaagccta aagcaggtat
     4681 gtttactcaa gcgattcaag atttaaacat cgacccaagc caatcttata tggtgggcga
     4741 taaattagaa gatatccttg ccgcagaagc cgccggcgtg aaaaccaaag tgctggttcg
     4801 cactgacaaa gcagtgacgg aagagggtca ggctaaagcc gatttggtgt tagatagttt
     4861 agttgattta gtaaaatata ttaaataaag caatgaatta taacgataaa tccctttccg
     4921 ccctgaaatt agggcaaaaa accgagtaca aaagcgaata tgatgcctcg cttttgcaac
     4981 ctgttcctcg taaattaaac cgtgatagtt taggcattac acaagagcag ccttttaacc
     5041 gaggggctga tgtgtggact tgctatgagc tatcttggtt gaaccaaaat gggctaccgc
     5101 aagtagcgat tgcagatgtc gccattgatt ttaagagcga aaacttaatt gaatcgaaaa
     5161 gtttcaagct ctatttgaac agttttaacc aaaccaaatt tagctcaata gaagaagttg
     5221 ggaaaacgat tcaagcggat ctggctcgct gtgcaagcgg tcaggtttcg gtaaaaattt
     5281 accaattatc acactacacg caacagccga tagttgattt tgcaggcgat tgcattgatg
     5341 agcaagatat tcaaatcgac agttatgcgt tttcgactca ttatttagag aacattgcag
     5401 aagatgagtt ggtggaagaa accttagtca gccatttgct caaatctaac tgcttaatta
     5461 cgtcacagcc tgactgggga agtgtgcaaa ttcgctacaa aggcaaaaag ctaaaccgtg
     5521 aaaaactgtt acgttattta gtgtcgttcc gtgagcataa tgagttccac gaacaatgtg
     5581 tggaacgcat tttctgtgat ttaatgcaat ttgccgagcc ggaaaaatta acagtttacg
     5641 cacgctacac cagacgaggc gggctagaca tcaatccgtt tagatcgaat tttgaggaag
     5701 tgccggaaaa tctaagaatg gcacgccaat gattttgttg ttttttgttc ccgtctgaac
     5761 cgcctgagcg tgtttgaatt ggtaggtaaa tttactctga gcggagcgaa tgtaaatttg
     5821 ccgttaagcc aattcaaact aagcgaggat agcggtgaag ctcgggttgc ctttcttttg
     5881 gttacttttc tttggcaaag caaagaaaag taacaaatag cagatgttga tttttatgat
     5941 gaatgaatat agcccaaatt actatatagg cgtaatgtcc ggcacgagcc ttgatggggt
     6001 ggatattgct ttgctggatt ttgcaaaaaa tccaccgaaa atgaccgctt gtgatttttt
     6061 tccaatgccg gaggaattac gggcggatat ttctgcgtta cttaaaaccg gcgaaaccaa
     6121 tctccaaaaa ctgggggaaa ttgatcaccg tttaggcttg ctttatgcag aaacgatcaa
     6181 tgcgttttta gcgaaaaatc agctaaaagc agaagatatt caagccatag gttgtcacgg
     6241 gcagacggtg tggcattcac cggattctga gtatcccttc accacccaaa ttggcgatat
     6301 gaatttggtg gcagagcaaa ccggtattac cactatcgct gattttcggc gtaaagatat
     6361 ggcagtaggt gggcaagggg ctccgcttgt gccggcattt catcaagcta tttttagcga
     6421 tccaaatcgt ctaacagtgg tgctgaatat tggcggcatc agcaatattt cggtgttaaa
     6481 tcccaattcc cctacagttg gttatgatgt aggagccgga aatactttga tggatagttg
     6541 gatcgaacaa catcaaggca aaagatacga taaaaacggc gaatgggcga gaacgggcaa
     6601 gctcaatact gaactgttag cgacgttatt agacgagtcg ttttttgcta aagccccgcc
     6661 taaaagcact gggcgggagc tgtttaattt aacgtggctt gcaaaaaaat tggaaaattt
     6721 gaccgcttgt cagccacaag atgtgcaacg cacgctggca gaatttactg ctcaaagtat
     6781 tgccaatgag ttattacgct ttgaaagctc tctgcctcgc ttgcttttag tttgtggtgg
     6841 tgggtcaaga aatccgttat taatggaacg gatttctgct ttacttcccg agtgggaggt
     6901 tacaaccact acagaatttg gtttagatat tgattatgta gaggctgccg cttttgcttg
     6961 gttagcttac cagcgaatcc ataatttacc cagtaatatg cctagcgtga caggagcaaa
     7021 aaaagccgtg agtttgggcg tgatttaccc tgcttagatt aagaaaatgt aaggtaatac
     7081 attcattact ccgagagtgg taatagctaa aattaattta gagcttccaa acattaagcc
     7141 ttggttttta ccattgtcta atttcactag catattagat agtgccgtat tcgcatacac
     7201 ggtaatagtg gcaaaaatca ctaaatagag aaatgcagtg atggttgaaa gccattggag
     7261 tgcataaaac caaggcatta tttgaagtat aatcagtatt ggtgcaatat aaataagttt
     7321 atgtactgtc agatttaatt ttcgtgaaaa atcagattgc aataacactg caattaaatt
     7381 attcacaaag aatgagacag agactaattc tgg
//
LOCUS       DANLXF010000117         7386 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.117, whole genome shotgun sequence.
ACCESSION   DANLXF010000117 DANLXF010000000
VERSION     DANLXF010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7386
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.117"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(49..1884)
                     /gene="glmS"
                     /locus_tag="PXD66_001597"
     CDS             complement(49..1884)
                     /gene="glmS"
                     /locus_tag="PXD66_001597"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDL6319884.1"
                     /translation="MCGIVGAVAQRDIAEILVDGLHRLEYRGYDSAGVAVLNADDKTM
                     QIVRRVGKVKALDEALDTKPLLGGTGIAHTRWATHGEPSETNAHPHRSGKIAVVHNGI
                     IENYEELKIVLQERGYVFQSQTDTEVIAHLVEWELRSASSLLEAVQKTVVQLRGAYGT
                     VVLNEEEPEHLIVARSGSPLVIGYGVGENFLASDPLALLSVTRRFAYLEEGDVAEITR
                     RTVDIYDREGNKAEREIHEGNFEADAADKGQYRHYMQKEIFEQPVAIMNTLDGRIKDG
                     KVDISAIAPNAAEILSKVEHVQIVACGTSYNAGMVSRYWFESIAGVSCDVEIASEFRY
                     RKFVTRPNSLLITLSQSGETADTLAALRLAKESGYMAAMTVCNVASSSLVRESDFAFL
                     TKAGVEIGVASTKAFTTQLTCLLLLNVAIGRLQGRMSEEQEHHIVQSLQRLPAQIESA
                     LVFDKQIEKLSEDFADKHHTLFLGRGEYYPVAMESALKLKEISYIHAEAYAAGELKHG
                     PLALIDSDMPVVVVAPENDLLEKVKSNIEEVRARGGQLYVFADHDAGFEEAEGFKTIV
                     MPKVDEVTAPIFYTVPLQLLSYHIALIKGTDVDQPRNLAKAVTVE"
     gene            complement(1932..2693)
                     /locus_tag="PXD66_001598"
     CDS             complement(1932..2693)
                     /locus_tag="PXD66_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDL6319885.1"
                     /translation="MSKRNTQQRRHLIVQMVQEKNEVSVDELAHFFETSEVTIRKDLT
                     ALEESGFLLRKYGGAVKIPSEMMEEAFIEQLSIQKKMIAQAARDCIRDHNRIIIDSGS
                     TTGALVKALNQTGLVVMTNSLNLATELTSLECEPTVLMTGGTWDSRSESFQGKVAEQV
                     LRSYDFDQLFIGADGLDLARGTTTFNELVGLSQVMAEVSREVIVLIESQKIGRKMPNI
                     ELKWQNITKIITDSQLSFETKEQIEKMGVEVVIAK"
     gene            complement(2850..3050)
                     /locus_tag="PXD66_001599"
     CDS             complement(2850..3050)
                     /locus_tag="PXD66_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDL6319886.1"
                     /translation="MKSILKVVGAMVLMVAVSGCNNMTKTQRNTAIGAAIGGVAGHAI
                     GESTGATLGGAALGGVIGSQIK"
     gene            3275..4309
                     /locus_tag="PXD66_001600"
     CDS             3275..4309
                     /locus_tag="PXD66_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL6319887.1"
                     /translation="MKLKKLSLALSTLLLGSAFANSALAEGRLTVYCSATNELCEQEV
                     QAFGKKYNVKVAFVRNGSGSTLAKIEAEKNNPQADVWYGGTLDPHSQAAEMGLLEPYK
                     SPNLEQIIEKFRDPAKLKGNYSSAVYMGILGYGVNLDRIKKLGIEKVPSTWEDLLDPR
                     LAGEIQIADPQSSGTAYTAIATFVQLLGEEKAFDYFRKLHKNISQYTKSGITPARNTA
                     RGETAIGIGFLHDYAIEKKNGANIEMTAPTDGTGYELGGVSILKGARNLDNAKLFVDW
                     SLSKEAQELSWQKGNAFQVLTNTTAESSPYALDPKNLNLINYDFEKYGSSEERKRLID
                     KWVNEVKLAK"
     gene            4377..6413
                     /locus_tag="PXD66_001601"
     CDS             4377..6413
                     /locus_tag="PXD66_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016534092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL6319888.1"
                     /translation="MQNFSFSRPLVWVLIGLLGFLLLPSKALDYGLFDSTSDELLAAM
                     GWASLNITWAWFLPLLVFLLPNSTACKTRAKLELVGLGVLFAFIFLSAIVLKISLGYA
                     VLPLIIAIMGLATPNLATLKVMQGDRFIIGSLLSIILLIFFFIVYPTIAIFVSMFYNG
                     SEFVPSQVLTILQQPYVVRVIGNSLSVAGTVGVLSTLFGLAFALYTTRIAKRSAFIGR
                     IFSILPIVTPPFVVGLGVTLMLGRSGYVTEFLVDYFGFANNWLYGFTGIVIAHTLALT
                     PMSFMILEGALKSIHPSVEEAAYTLRSNRYQAFFNIIFPLLKPALANSFLVVAIQSLA
                     DFSTPLVLGGSFDVIATQIYFYIAGSQLDYASASTLGAILLVFSLAIFVIQYLWIGNR
                     SYVTVSGKSYRGDVQDLPSGMKWLIICTLLFWGTFNLVLYGSIFYGSFTVNWGVDYTL
                     TLDNYIKLFGQGFSDGGFPSLIQTVIFAAAAAPITALFGLLIAYVTVRRDFKGKKTLE
                     FLTLLCFAVPGTVAGVSYILAFNDAPIYLTGTGMIIILSMVMRNMPVGMRAAIAGLGQ
                     LDKSLDEASLSLKAGSFKTMLFVVLPLLKPALLSALVTSFVRAMTTVSAIVFLVTADT
                     RVATSYILNRVEDGEYGIAIAYGSILIVVMMAIILIFDWIVGDTRIARSKAKKM"
     gene            6511..>7386
                     /gene="fbpC"
                     /locus_tag="PXD66_001602"
     CDS             6511..>7386
                     /gene="fbpC"
                     /locus_tag="PXD66_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005700953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric ABC transporter ATP-binding protein"
                     /protein_id="HDL6319889.1"
                     /translation="MNNDFLVLKNVTKSFGKVTVIDNLNLSIPQGKMVTLLGPSGCGK
                     TTVLRLVAGLENPTSGQIFIDGEDVTQSSIQHRDICIVFQSYALFPHMSIGDNVGYGL
                     KMQGVPKEERLQRVKEALELVDLGGFEDRFVDQISGGQQQRVALARALILKPKVLLFD
                     EPLSNLDANLRRSMREKIRELQQRLNITSLYVTHDQSEAFAVSDEVIVMNKGKIMQKA
                     PAKELYLRPNSLFLANFMGESSIFEGTIAGNQININGYQFTLANAEQFELPDGEYLVG
                     IRPEAIHLAEQGNISQ"
BASE COUNT         1987 a         1693 c         1507 g         2199 t
ORIGIN      
        1 cctataactc atataaaaac cccaagatat cttggggttt aactaacatt actctacagt
       61 caccgctttt gcaaggttac gaggttggtc aacgtccgtt cctttgatta aggcaatgtg
      121 atatgaaagt agttgtaacg gcacggtgta gaaaatcggt gcggttactt catcaacttt
      181 cggcattaca atcgttttga agccttctgc ttcttcaaaa cctgcatcgt gatcggcaaa
      241 cacatataac tgaccgcctc ttgcacgcac ttcttcaata ttggatttca ctttctcaag
      301 caagtcattt tccggtgcta ccaccactac cggcatatcg ctatcaatta atgctaacgg
      361 gccgtgtttt aactcgcctg cggcataagc ttctgcgtga atatatgaaa tctcttttaa
      421 tttcaatgca gattccatcg caaccgggta atattctcca cgacctaaga acaatgtgtg
      481 gtgtttatcc gcaaaatctt ccgataattt ttcgatttgt ttatcgaaca ctaacgcact
      541 ttcaatttgt gctggtaagc gttgtaaaga ttgcacgata tggtgttctt gctcttcact
      601 cattctgcct tgtaagcgtc caatcgccac atttaataac aataagcaag ttaattgagt
      661 agtgaatgct ttggttgatg ctacaccaat ttcaacacca gctttggtta agaatgcaaa
      721 atcggattca cgcactaatg aagaacttgc cacattacac accgtcattg ccgccatata
      781 gccagattct ttcgctaaac gcagtgcagc taacgtatct gcggtttcac ctgattgtga
      841 aagcgtgatt aataagctgt tcgggcgagt aacgaattta cggtaacgga attcggaggc
      901 aatttccaca tcacagctca cgcctgcaat ggattcaaac caataacgtg aaaccatacc
      961 agcattgtaa gaggttccac aagccacaat ttgcacgtgt tctactttcg ataaaatctc
     1021 tgccgcattc ggtgcaatgg ctgaaatatc tactttaccg tctttgatac gaccatctaa
     1081 ggtattcata atcgcaaccg gctgctcaaa aatctctttt tgcatatagt gacgatattg
     1141 tcctttatct gccgcgtctg cctcaaaatt gccttcgtga atttcacgct ctgccttgtt
     1201 gccttcacgg tcataaatat ccactgtgcg acgggtaatt tcggctacat caccttcttc
     1261 tagataagca aaacggcgag ttacgcttaa taatgcaagt gggtctgaag caaggaagtt
     1321 ctcgcctaca ccgtaaccaa tcactaacgg gctacctgag cgagccacaa ttaaatgctc
     1381 cggctcttct tcatttaata ccactgtgcc gtaagcacca cgcagttgca ccacggtttt
     1441 ttgtaccgct tccaataatg aagaagcaga acgcaattcc cattcaacta aatgagcaat
     1501 gacttcggta tcggtttgag attggaacac ataaccacgc tcttgtaaca ctatttttaa
     1561 ctcttcgtag ttttcgataa tcccgttatg aactactgca attttgccac tgcggtgtgg
     1621 gtgagcatta gtttctgacg gctcgccgtg cgttgcccag cgagtgtgtg cgatcccagt
     1681 gccgcctaat agtggcttag tgtctaacgc ctcatctaag gctttgactt taccgacacg
     1741 gcgtacaatt tgcattgttt tatcatctgc atttaatact gctacaccag cagagtcata
     1801 tccacggtat tctaagcgat gtaaaccatc aaccaagatt tctgcgatat cacgttgtgc
     1861 tactgcacct acaattccac acataaattt gctttcctat gtttaagttt aaaaattcat
     1921 ttgagctact gctattttgc aataaccact tcgacaccca ttttttcgat ttgttctttc
     1981 gtttcaaaac ttaattgact gtcggtaata attttggtaa tattttgcca tttcaattca
     2041 atattcggca ttttccgacc gattttttgc gattcaatta acacaatcac ttcacggctc
     2101 acttctgcca tcacctgact taagccaact aattcattaa aggtagtcgt gcctcgagcc
     2161 aaatctaaac cgtccgcacc aataaaaagc tgatcgaaat catatgaacg caatacctgt
     2221 tctgctactt tgccttgaaa ggactcagaa cggctatccc aagtgccgcc ggtcatcaac
     2281 actgttggtt cgcattcgag ggaagtcaat tccgtcgcta aattcagcga atttgtcatc
     2341 accaccaagc cggtttggtt caatgctttt accaaagctc cggtcgtgct gccgctgtca
     2401 ataatgatgc gattatgatc acgaatacaa tcacgtgcgg cttgagcaat cattttcttt
     2461 tgaatcgaaa gttgctcaat aaacgcttct tccatcattt ccgatggaat ttttaccgcc
     2521 ccaccatact tacgcaataa aaaaccactt tcttccaaag cggttaaatc ttttctaatc
     2581 gtaacttcag acgtctcaaa gaaatgagcg agttcatcta cactcacttc atttttctct
     2641 tgtaccattt gaacaattaa atggcggcgt tgttgcgtgt tacgttttga cattataatc
     2701 ttcacttaaa tttcgtttca aaacttttgc attataaaga aagctcgcga aaagaaaagc
     2761 agaaaatgca aaatttacaa aatctataca aaataaaacc gcttgtaata ttgatttaca
     2821 agcggttgat ttcttgaaat tttttgcaat tatttgattt ggctaccaat cacgccacca
     2881 agtgcggcac cacctaaagt tgcaccggta gattcaccaa tcgcgtgacc agccacaccg
     2941 ccaattgctg caccgattgc agtattacgt tgcgtttttg tcatattgtt acagccagac
     3001 acagcaacca ttaataccat tgcaccaaca actttcaaaa tagatttcat aataatccct
     3061 cataattaat caatttcaaa taagccaata ttcttggcta tgctataaga tttaatttat
     3121 ttagaaaggt tcaactacta ccacctatga attgcaaatt ttttcacaat ttaggccgct
     3181 tgttgattga actgtgagct tgctcacaga attttatggt atttcttgct atattgtata
     3241 gcaatctatg cgataactca ttaaggaaat aaaaatgaaa ttaaaaaaat tatctttagc
     3301 actctcgact ttattactcg gttcagcctt tgccaactct gctttagcgg aaggtcgttt
     3361 aaccgtttat tgtagtgcca ctaatgaact ttgtgagcaa gaagtgcaag cattcggcaa
     3421 aaaatataat gttaaagtag catttgtgcg taatggttca ggcagtacat tggcgaaaat
     3481 tgaagcggag aaaaacaatc ctcaagctga tgtatggtat ggcggtactt tagatccaca
     3541 ttcacaagct gcagaaatgg gtttactcga gccgtataaa tcaccgaatc tcgaacaaat
     3601 tatcgaaaaa ttccgtgatc cggcaaaatt aaaaggcaac tactcttcgg ctgtgtatat
     3661 ggggatttta ggttatggtg tgaatttaga ccgaatcaaa aaactcggca ttgaaaaagt
     3721 gcctagcact tgggaagact tgctcgaccc acgcctagcc ggtgaaatcc aaattgccga
     3781 cccacaaagc tccggcacgg cttatactgc gattgcgacc ttcgtacaac ttttaggaga
     3841 agaaaaagcc ttcgactatt tccgcaaatt acacaaaaac atttcacaat ataccaaatc
     3901 cggcatcaca ccggcacgca ataccgcccg tggtgaaacc gctatcggta tcggtttctt
     3961 acacgactat gcgattgaga agaaaaacgg ggctaacatt gaaatgaccg caccaacaga
     4021 cggcacaggc tacgaattag gtggtgtgag tatcctcaaa ggtgcaagaa atcttgataa
     4081 tgcaaaactg ttcgtggatt ggtcgctttc taaagaagct caagaactct cttggcaaaa
     4141 aggcaatgcg ttccaagtat taaccaacac caccgcagaa tcttcccctt atgctctaga
     4201 tccgaaaaac ttaaacttaa tcaactatga tttcgagaaa tacggctcga gcgaagagcg
     4261 taaacgcttg attgataagt gggtaaatga agtaaaatta gcgaaatagt gttaatcttt
     4321 ccactgaggg gataagcaat tatcccctct ctaattataa gaatctgtga ggtgttatgc
     4381 agaatttttc cttttcccga ccgcttgttt gggtgcttat cgggttactc ggttttctct
     4441 tgttaccgtc taaagcctta gattatgggc tgtttgactc aacctctgat gaactgctcg
     4501 ccgcaatggg ctgggcgagt ctgaacatca cttgggcgtg gtttttaccg cttttggtat
     4561 ttttattgcc aaattcgacc gcttgtaaaa ctagagccaa gctggaatta gtagggctgg
     4621 gcgtgctgtt cgcctttatt ttcctgtcag cgattgtgct gaaaatcagc ctcggttatg
     4681 cggtgttgcc gttgattatt gccattatgg ggcttgcgac cccaaattta gcaacgctca
     4741 aagtaatgca gggtgaccgc tttattatcg gctcgttact gagcattatt ctgctgattt
     4801 tcttttttat tgtgtatccg accatcgcca tttttgtgtc gatgttctac aacggctcgg
     4861 aatttgtgcc aagccaagtg ctgacaattt tgcaacaacc ttatgtcgtt cgagtcatcg
     4921 gtaactcgct ctcggttgcc ggcacggtgg gcgttctctc caccctattc ggcttagcct
     4981 ttgctctcta caccactcgg attgctaaac gctcggcgtt tatcgggcgg attttctcga
     5041 ttttacctat cgtcaccccg ccgtttgtgg tcggtttagg cgtaaccctg atgcttgggc
     5101 gttccggcta tgttactgaa tttttagtgg attactttgg ctttgccaac aattggctgt
     5161 acggtttcac cggtattgtg atagcccata ccctcgcgct cacgccaatg tcgtttatga
     5221 ttttggaagg ggcattgaaa tccattcacc catcggtgga agaagcagct tacaccctgc
     5281 gttccaaccg ctatcaagcc ttttttaaca ttattttccc attgttaaaa ccggcattgg
     5341 caaactcctt tttagtggtg gcaattcaat ccctcgccga tttcagtacg ccgttagtat
     5401 tgggcggtag ctttgatgtg attgcaaccc aaatctactt ctacattgcc ggttctcaac
     5461 tggattatgc ctctgctagc acgcttgggg caattttgtt ggtgttctcc ttagccattt
     5521 tcgtgatcca atacctctgg atcggcaatc gctcttatgt gacggtgtcc ggcaaatcct
     5581 atcggggtga tgtccaagac ttaccaagcg gtatgaaatg gctgattatt tgcaccttat
     5641 tgttctgggg aaccttcaac ttagtgctgt acggcagtat tttctacggc agtttcaccg
     5701 tgaactgggg tgtggattat accttaaccc ttgataacta catcaagcta ttcgggcaag
     5761 ggtttagcga tggcggtttc ccttcgttaa ttcaaaccgt gattttcgct gccgctgccg
     5821 ccccgattac tgcactattc ggcttgctga tcgcctatgt gacagttcgg cgtgatttca
     5881 aaggcaagaa aaccctggaa tttttaaccc tgctctgctt tgccgtaccg ggaacggtgg
     5941 caggtgtttc ttacattctc gcctttaacg atgccccgat ttacctaacc ggcaccggta
     6001 tgatcatcat tctctcaatg gtaatgcgga atatgcctgt gggtatgcga gcggcaattg
     6061 ccggcttagg gcagttggat aaatcgctgg acgaagcctc gctctcgctg aaagccggtt
     6121 cgttcaaaac gatgctgttt gtggtgttac cgctactcaa accggcactg ctctccgccc
     6181 tcgtcaccag ctttgtgcgg gcaatgacga ctgtcagtgc gattgtattc ttagtcaccg
     6241 ccgacactcg tgtggcaacc tcctacattc tcaaccgtgt ggaagacggt gaatacggca
     6301 ttgcgattgc ctacggctca attttaattg tggtgatgat ggcgattatc ctgatttttg
     6361 actggattgt cggcgacacc cgtatcgccc gttctaaggc gaaaaaaatg taaaaagaca
     6421 tctactctct ccctttatgg gagagagaca gaaaaatact tcgttcagaa gtatttttca
     6481 gagagaggga tataattaaa aggacaatat atgaacaacg atttcttagt attaaaaaac
     6541 gtcaccaaat ctttcggcaa ggtgacggtc attgataatc tcaacctcag tattccgcaa
     6601 ggtaaaatgg tgacattact cgggccgtcc ggctgtggta aaaccaccgt actgcgttta
     6661 gtggcagggc tggaaaaccc gacttcgggg caaattttta ttgatggcga agatgttacc
     6721 caaagttcga tccaacaccg tgatatttgc attgtgttcc aatcttatgc gttatttccg
     6781 catatgtcga ttggcgataa tgtcggctac ggcttgaaaa tgcagggcgt accgaaagaa
     6841 gaacggctgc aacgagtgaa agaggcgttg gaattggtgg atttaggcgg ctttgaagat
     6901 cgctttgtcg atcaaatctc tggcggacaa caacaacgtg tcgccctcgc ccgtgcctta
     6961 attctgaaac cgaaagtgct actgttcgat gaaccgctga gtaaccttga cgctaactta
     7021 cgccgttcaa tgcgggagaa aatccgtgaa ttacagcaac gtttaaacat tacttccctt
     7081 tatgtgactc acgatcagtc cgaagccttt gcggtttcag atgaagtaat tgtgatgaac
     7141 aagggaaaaa ttatgcaaaa agctccagcc aaagagttat accttcgccc taattcttta
     7201 ttcttagcaa attttatggg tgaaagcagc atttttgaag gcacgatagc tggcaaccaa
     7261 attaatataa atggatatca atttacgtta gcaaatgctg aacaatttga gttacccgat
     7321 ggggaatact tagttggcat tcgtccagaa gctattcatt tagctgaaca aggaaatata
     7381 agtcaa
//
LOCUS       DANLXF010000118         7359 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.118, whole genome shotgun sequence.
ACCESSION   DANLXF010000118 DANLXF010000000
VERSION     DANLXF010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7359
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.118"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            26..445
                     /locus_tag="PXD66_001603"
     CDS             26..445
                     /locus_tag="PXD66_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005704641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS sensor domain-containing protein"
                     /protein_id="HDL6319890.1"
                     /translation="MVPSNEAVLKAVHDTLVISTTDLQGVITYANDLFCKLTGFTREE
                     LIGQPHNIVRHPAVPKEVYKDMWDTIQAGKIWTGIVPNCGKGGVVYVVDTTVQPMFDE
                     TGKITGYISVRRVINDLMTNFEAVESAKEIFDDFYGE"
     gene            516..2072
                     /locus_tag="PXD66_001604"
     CDS             516..2072
                     /locus_tag="PXD66_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase flavoprotein subunit alpha"
                     /protein_id="HDL6319891.1"
                     /translation="MSLIPHIHIAYGSESGNAEKLAQQLAIQPFLLPYSPTLSTLNET
                     DLSQLSAESLLLIVTSSFGDGEPPENADEFAEKLENLTACNLQYAIFGLGDITYDKFC
                     GYSKQLDSLLQQKQAKAVIARVDADLNYQEIFKQWLPLVEQALHSTNKETIQHSLSVQ
                     AYGEEMAYQAEVLEIKHLANSSPAVYHIRLSLKESGIFYQAGDLIYVKVQQPDRLLNE
                     YAEWFNDQNAVALLRHKELRLLSKNVLRDIQKICGSNELKDLTKISNKKALEQYVYGR
                     DLLDVLRDFDPNKSVTLADLEPILSNLAARAYSISSCGKTHPDYVDLCIRHVSYHLNG
                     RDYQGTASGYLSELQAGDFVSIFANANPNFRLPETLTAPVVMIGSGTGIAPHIAFLQA
                     LEQQYQGVESYLFFGERYRDKDFLYQTELENYLAKGTLTQLFTAFSRDQAEKFYVQDA
                     LSQQAELVWHLIQQGAYFYICGSKAMSKAIDAELIKIADQIGGDPYIDDFNNIIAKLV
                     AEGRLMRDVY"
     gene            complement(2130..2387)
                     /gene="rpsQ"
                     /locus_tag="PXD66_001605"
     CDS             complement(2130..2387)
                     /gene="rpsQ"
                     /locus_tag="PXD66_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005759643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDL6319892.1"
                     /translation="MTDKIRTVQGKVVSDKMDKSFVVAIERTVKHPLYGKFIRRTTKL
                     HVHDENNEAKLGDVVEIKECRPISKTKSWTLVRVVEKAVVA"
     gene            complement(2387..2578)
                     /gene="rpmC"
                     /locus_tag="PXD66_001606"
     CDS             complement(2387..2578)
                     /gene="rpmC"
                     /locus_tag="PXD66_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDL6319893.1"
                     /translation="MKAQELRNKSVEELNGELVNLLGEQFKLRMQAATGQLQQTHQLK
                     QVRRSIAQVKTVLTEKAGE"
     gene            complement(2578..2988)
                     /gene="rplP"
                     /locus_tag="PXD66_001607"
     CDS             complement(2578..2988)
                     /gene="rplP"
                     /locus_tag="PXD66_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDL6319894.1"
                     /translation="MLQPKRTKFRKVHKGRNRGIAGGTEVSFGSFGLKAIGRGRLTAR
                     QIEAARRAMSRAVKRQGKIWIRVFPDKPITEKPLEVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVSEEVARNAFALAAAKLPFKTTFVTKTVM"
     gene            complement(3002..3709)
                     /gene="rpsC"
                     /locus_tag="PXD66_001608"
     CDS             complement(3002..3709)
                     /gene="rpsC"
                     /locus_tag="PXD66_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDL6319895.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTQDFADNLDGDFKVRKFLN
                     KELANASVSRITIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRNAVAKIAGVPAQIN
                     IAEVKKPELDAKLVADSIASQLERRVMFRRAMKKAVQNAMKLGAKGIKVEVSGRLGGA
                     EIARSEWYREGRVPLHTLRADIDYSTAEAHTTYGVIGVKVWIFKGEILGGMAAVIESE
                     KEPAAQPKKAPRGKGRK"
     gene            complement(3729..4061)
                     /gene="rplV"
                     /locus_tag="PXD66_001609"
     CDS             complement(3729..4061)
                     /gene="rplV"
                     /locus_tag="PXD66_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDL6319896.1"
                     /translation="METIAKHRYARTSAQKARLVADLIRGKKVSVALEILTFTNKKAA
                     TLVKKVLESAIANAEHNDGADVDDLKVAKIFVDEGPSMKRVMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            complement(4072..4347)
                     /gene="rpsS"
                     /locus_tag="PXD66_001610"
     CDS             complement(4072..4347)
                     /gene="rpsS"
                     /locus_tag="PXD66_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDL6319897.1"
                     /translation="MPRSLKKGPFLDLHLLKKVEKAVESGDKKPIKTWSRRSMIIPSM
                     IGLTIAVHNGRQHVPVYVSDEMIGHKLGEFAPTRTYRGHAADKKAKK"
     gene            complement(4378..5199)
                     /gene="rplB"
                     /locus_tag="PXD66_001611"
     CDS             complement(4378..5199)
                     /gene="rplB"
                     /locus_tag="PXD66_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDL6319898.1"
                     /translation="MAIVKCKPTSAGRRHVVKVVNAELHKGKPFAALLDTKSKTGGRN
                     NLGRITTRHIGGGHKQHYRLIDFKRNKLDIPGVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLTAGDQIQAGASAPIKVGNALPMRNIPVGTTVHNVELKPGKGGQIARSAGA
                     YVQIIAREGNYVTLRLRSGEMRKVLAECTATIGEVGNSEHMLRVLGKAGANRWRGIRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRHNKRTDKFIVRRRGK"
     gene            complement(5218..5523)
                     /gene="rplW"
                     /locus_tag="PXD66_001612"
     CDS             complement(5218..5523)
                     /gene="rplW"
                     /locus_tag="PXD66_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDL6319899.1"
                     /translation="MIQQERLLNVLKAPHISEKATNNAEKGNTIVFKVALDANKVEIA
                     NAVEELFEVKVDSVRTVVVKGKTKRRGAKVGRRSDWKKAYVTLQEGQSLDFVEGAAE"
     gene            complement(5520..6122)
                     /gene="rplD"
                     /locus_tag="PXD66_001613"
     CDS             complement(5520..6122)
                     /gene="rplD"
                     /locus_tag="PXD66_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDL6319900.1"
                     /translation="MELQVVGANALTVSETTFGREFNEALIHQVVVAYAAGARQGSRA
                     QKTRAEVSGSGKKPWRQKGTGRARSGDIKSPIWRSGSVTFAAKPQDHSQKVNKRMYRG
                     AIKSILSELVRQDRLVVVEKFEIDAPKTKVLAQKLKDMALNDALIITANVDENLFLAA
                     RNLYKVDVRDVQGIDPVSLIAFDKVVVTVDAVKQIEEMLA"
     gene            complement(6138..6767)
                     /gene="rplC"
                     /locus_tag="PXD66_001614"
     CDS             complement(6138..6767)
                     /gene="rplC"
                     /locus_tag="PXD66_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDL6319901.1"
                     /translation="MIGLIGRKVGMTRVFTEDGVSIPVTVIEIEANRVTQVKTLENDG
                     YSAIQVTTGSKKASRVTKPEAGHFVKAGVEAGRGLWEFRTTEGEEFTLGQEINVDIFT
                     DVKKVDVTGTSKGKGFQGGVKRWNFRTQDATHGNSLSHRVLGSIGQNQTPGRVFKGKK
                     MAGHLGAERVTVQSLEVVRVDVERKLLLVKGAVPGATNGDVIVKPAVKA"
     gene            complement(6793..7104)
                     /gene="rpsJ"
                     /locus_tag="PXD66_001615"
     CDS             complement(6793..7104)
                     /gene="rpsJ"
                     /locus_tag="PXD66_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDL6319902.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQSTAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHKRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
BASE COUNT         2137 a         1564 c         1366 g         2292 t
ORIGIN      
        1 atggagattc aacacggctg aacaaattgt tccttcaaat gaagctgttt taaaagcagt
       61 ccacgatact ttagttattt caacaactga tttacaaggt gttattactt acgctaacga
      121 ccttttctgc aaattaaccg gttttacccg tgaagaatta attggacaac cgcataatat
      181 cgtacgccac ccagctgtac caaaagaagt ttataaagat atgtgggata caatccaagc
      241 cggtaaaatc tggacaggta tcgtaccaaa ctgcggtaaa ggcggtgtag tttatgtggt
      301 tgataccacc gttcaaccaa tgtttgatga aacaggcaaa attacaggct atatcagcgt
      361 tcgccgtgta attaatgatt taatgaccaa ctttgaagca gtagaatcag caaaagaaat
      421 ctttgatgac ttctacggtg aataattaaa aaacaatgat tgcgtgtaaa agatgatctc
      481 ttttacacgc aattttttat tttcgaggag tttgtatgtc tctcattccc catatccaca
      541 ttgcttatgg ttcggagtct ggtaacgcag aaaagcttgc ccaacaatta gctatccaac
      601 cctttttact tccctactca cccacacttt ctacattaaa cgaaaccgat ttaagccaat
      661 tatctgccga gagtttattg cttattgtaa ccagctcctt tggtgatggc gaaccacctg
      721 aaaacgccga tgaatttgca gaaaaattgg aaaatttgac cgcttgtaat ctgcaatatg
      781 cgatattcgg gctaggtgat attacttatg ataaattctg tggctatagc aaacaactag
      841 attcattatt acaacaaaaa caagctaaag ctgtgattgc aagggttgat gccgatttaa
      901 actatcaaga aatttttaaa caatggctac ccctagtgga gcaggcatta cattcaacaa
      961 acaaagaaac gatccaacat tccctttctg ttcaagctta cggcgaggaa atggcatacc
     1021 aagcagaagt gttggaaatt aagcatttgg caaactcaag ccctgcggtg tatcacattc
     1081 gtttatcatt aaaagagagc ggtattttct atcaagcagg ggatttaatt tatgtcaaag
     1141 tgcagcaacc agacagacta ttaaatgaat atgcagaatg gtttaacgat caaaatgcag
     1201 ttgcattact acgccataaa gaactccgac tactaagtaa aaatgtactg cgtgatattc
     1261 aaaaaatttg cggcagtaat gaactcaaag atttaactaa aatttccaat aaaaaagcat
     1321 tggaacaata tgtctatggg cgggatttat tagacgtatt acgcgatttt gacccaaaca
     1381 aaagcgtgac attagccgat ttagagccaa ttttatctaa cttagccgca cgagcctatt
     1441 caattagctc ttgcggtaaa actcaccctg attatgttga tttatgtatc cgccacgttt
     1501 cgtatcattt aaacggtaga gattatcaag ggactgcaag tgggtattta tccgaattac
     1561 aagcgggcga ttttgtctcg atttttgcaa atgccaatcc gaatttccgt ttacccgaaa
     1621 cattaaccgc tccggtggtg atgattggct caggtactgg cattgctccg catatcgcat
     1681 ttttacaagc gttagagcag caatatcagg gggttgaaag ctatttattc ttcggcgagc
     1741 gttatcgtga taaagatttt ctgtatcaaa cggaactgga aaattactta gcaaaaggca
     1801 ccttaactca gctgtttact gctttttcac gtgatcaggc agagaagttt tatgtgcaag
     1861 atgccctatc gcaacaagca gagttagttt ggcatttaat tcagcaaggg gcttatttct
     1921 atatttgtgg tagcaaagca atgagtaaag caatagatgc ggaactcatc aaaattgcag
     1981 atcaaattgg aggcgatcct tatattgatg atttcaataa tattattgca aaattggtcg
     2041 cagaaggccg attaatgcga gatgtatatt agcaaatgaa aaaaactaaa aacccagcaa
     2101 aagctgggtt tttatttaat catagttcat taagcaacaa ctgctttctc aactacacga
     2161 actaaagtcc aagatttagt ttttgaaata ggacgacatt ccttaatttc aacaacatca
     2221 cctaatttag cttcattgtt ttcatcgtgt acatgaagtt tagtagtacg acggataaac
     2281 ttaccgtata acggatgttt aactgtacgt tcgatagcaa caacaaatga tttatccatt
     2341 ttgtcgctaa ccactttacc ttgtactgta cgaattttat cagtcattat tcacccgcct
     2401 tttcggttaa tacagtttta acttgtgcaa tactacgacg cacttgtttt aactggtggg
     2461 tttgttgaag ctgaccggtg gctgcttgca tacgcaattt gaattgttca cctaaaaggt
     2521 tcactaactc accattcagc tcttcaacac ttttattacg tagttcttga gctttcatta
     2581 catcaccgtc ttagttacaa atgtggtttt gaacggaagt ttagctgccg ctaatgcaaa
     2641 cgcattgcgt gcaacttctt cagaaacacc atccatttca tagagaactt tacccggttg
     2701 gattaaggct acccagtact caacgttacc tttaccttta cccatacgga cttctaatgg
     2761 tttttcagta attggtttgt ctgggaacac acggatccag attttacctt gacgtttaac
     2821 cgcacggctc atcgcacgac gagcagcttc aatttgacga gctgttaaac gaccacggcc
     2881 aatagctttt aaaccaaatg aaccgaagct cacttctgtg ccacccgcga taccacggtt
     2941 acggcctttg tgtactttac ggaattttgt gcgttttggt tgtaacattc agtgattctc
     3001 cttatttacg acctttgcca cgtggcgcct ttttaggctg tgccgctggt tctttttcag
     3061 attcaatcac tgcagccatt ccaccaagaa tttcaccttt gaagatccac acttttacgc
     3121 cgattacgcc gtaagtagtg tgagcttcag cagtactata atcgatgtct gcacgaagcg
     3181 tatgtagagg tacacgacct tcacgatacc attctgagcg agcaatttct gcaccaccta
     3241 aacgaccgct aacttcaact ttgatacctt tagcacctaa tttcatcgcg ttttgaactg
     3301 cttttttcat tgcacgacgg aacatcacac gacgttcaag ttgagaagcg atgctatctg
     3361 cgactaattt cgcatctagc tctggttttt tcacttcagc gatgttgatt tgagctggaa
     3421 cgccagcgat tttcgcaact gcgttgcgta atttttcaac atcttcacct tttttaccga
     3481 taacgatacc cgggcgagct gtgtgaatag ttacacgaat gctttttgca ggacgctcaa
     3541 tagtgatgcg tgatacagaa gcgttcgcta actctttgtt taaaaattta cgtactttga
     3601 agtcgccgtc taagttatca gcgaaatctt gtgtattcgc gaaccaagta gagttccaag
     3661 gtttaacaat acctaggcga ataccattag gatgtacttt ctgacccatt tgttattctc
     3721 ctacttaatt aacgatctga cacaaccaca gtgatgtggc ttgtacgttt taaaatacga
     3781 tctgcacgac cttttgcacg tggcattaca cgtttcatgc ttggaccttc atcaacgaag
     3841 attttagcaa ctttaagatc atcgacatct gcaccgtcat tatgctctgc gtttgcaata
     3901 gctgactcta atactttctt aacaagcgtc gctgcttttt tgttagtgaa agttaagatt
     3961 tctaatgcta cagaaacttt tttaccgcga attaaatcag caactaagcg agctttttgg
     4021 gcagaagtac gagcgtaacg atgtttagca atagtttcca tcttttacct cttatttctt
     4081 agctttctta tctgcggcgt gaccgcggta tgtacgagtc ggtgcaaatt cacctaattt
     4141 atgaccgatc atttcgtcgg aaacataaac aggaacgtgc tgacgaccat tatggactgc
     4201 gatggtcaaa ccaatcattg atggaatgat cattgaacga cgggaccaag ttttaattgg
     4261 ttttttatcc ccgctttcca ccgccttctc taccttcttc aacaagtgta ggtcaaggaa
     4321 aggacctttc ttgagggaac gtggcattgg tgtttacctt ttagttaatg aattaaatta
     4381 tttgccacga cgacgtacga tgaatttatc agtacgtttg ttgtgacgag ttttcttacc
     4441 tttggtttga acgccccaag gtgtaacagg atgtttaccg aagttacgac cttcaccacc
     4501 accgtgtggg tgatctaccg ggttcattgc agtaccacga acagttggac gaatacctct
     4561 ccaacggttt gcacctgctt taccaagaac gcgaagcata tgctctgagt tacctacttc
     4621 accaatggtt gcagtacact cagctaatac tttacgcatt tcgcctgaac gaagacgtaa
     4681 agttacatag ttaccttcac gggcaatgat ttgaacgtaa gcaccagcag aacgtgcaat
     4741 ttgaccacct ttaccaggtt ttaattcaac gttgtgtaca gttgtaccta ctgggatgtt
     4801 acgcattggt aatgcgttac ctactttaat cggagctgaa gcacccgctt gaatttgatc
     4861 accagcagtt aaaccttttg gtgctaaaat atagcgacgt tcaccatctt tataaagtac
     4921 taatgcgata ttcgcagaac gatttggatc gtattctaaa cgctcaacaa cacccggaat
     4981 gtctaattta ttacgtttaa aatcaattaa acggtagtgt tgtttgtgac cgccaccaat
     5041 gtgacgagtt gtgatacgac ctaagttgtt acgaccacca gttttagatt tagtatctaa
     5101 aagtgctgca aaaggtttac ctttgtgaag ctcagcgttt acaactttaa caacgtgacg
     5161 acgaccagcg gaggtcggct tacatttaac gatagccatt tattttatct cctctgatta
     5221 ctctgccgca ccttcaacga agtcaagcga ttgaccttct tgaagagtta cataagcttt
     5281 tttccagtca ctacgacgac caactttagc accacggcgt ttagttttac ctttaactac
     5341 tacggtacga acagaatcaa cttttacttc aaaaagttct tcaactgcat ttgcaatctc
     5401 aactttgttt gcatctaatg caactttgaa tacgattgta ttgccttttt ctgcgttgtt
     5461 tgtcgctttt tcagagatat gtggtgcttt aagcacgttt agcaaacgtt cttgttgaat
     5521 catgctaaca tctcctcaat ttgttttact gcatctacgg ttacaaccac tttatcgaaa
     5581 gcaattaagc taactgggtc aataccttgc acatcacgca catcaacttt atataagttg
     5641 cgtgctgcta agaataaatt ctcatctacg tttgcagtaa tgataagagc gtcatttaac
     5701 gccatatctt ttaatttttg tgctaatact ttagtttttg gtgcatcgat ttcgaatttc
     5761 tcaacaacca ctaaacggtc ttgacgaact aattcagaaa gaatgctttt gattgcacca
     5821 cggtacattc ttttgttcac tttttggctg tgatcttgtg gtttcgcagc aaaagttaca
     5881 ctacccgaac gccaaattgg tgatttaata tcaccagaac gtgcacggcc tgtacctttt
     5941 tgacgccaag gttttttacc tgaaccagac acttcagcac gagttttttg agcacgagat
     6001 ccttgacgag cacctgctgc ataagcaaca actacttggt ggattaatgc ttcattgaac
     6061 tcacgtccga aggtagtttc agaaacagtt agtgcgtttg cacctacaac ttgtaattcc
     6121 atctctatct cctagactta tgctttaact gccggtttta cgataacatc accattagtt
     6181 gcaccaggaa cggcaccttt aactaataat aatttacgct caacatctac acgaacaact
     6241 tcaagtgatt gaacggttac acgctctgca cctaagtgtc ctgccatttt tttaccttta
     6301 aacacacgac ctggagtttg gttttgacca atagaaccaa gtacacgatg tgataaagag
     6361 ttaccgtgag ttgcatcttg agtacggaag ttccaacgtt taacaccacc ttggaagcct
     6421 ttaccttttg atgtaccagt aacatcgact tttttaacat cagtaaagat gtcaacattg
     6481 atttcttgac ctaaagtgaa ttcttcacct tcagtagtac gaaattccca taaaccgcgt
     6541 ccagcttcaa cacctgcttt cacgaaatga cctgcttctg gtttagttac acggctagct
     6601 tttttagagc cagtagtaac ttgaattgca gagtagccat cgttttcaag ggttttaact
     6661 tgagtaacac ggttggcttc gatttcgata acggtaactg gaatagacac gccgtcttca
     6721 gtgaaaacgc gggtcatacc aactttacga ccgattaaac caatcattgt aataacctct
     6781 taaaattccc aattaaccta gactaatctg aacgtcaacg ccagcagcta gatctaaacg
     6841 cattaatgca tcaacagttt tttctgttgg ttcaacaata tcaactaaac gtttgtgagt
     6901 acggatttca tattggtcac gtgcatcttt attcacgtgt ggagaaatca atacagtgaa
     6961 acgttcttta cgagttggta aaggaattgg accacgaact tgtgcaccag tacgtttagc
     7021 tgtttctacg atctccgcag tagattgatc aattaaacga tgatcaaatg cttttaagcg
     7081 gatacggatt ctttggttct gcattagacc agagctccaa ttaaaaaatt tagctaataa
     7141 aaaaaccaaa ctaccactta acaaattagt taagggagct cagttaaacc tatatagttc
     7201 caaaattagg aacattgtat acactacaat atctgtagtg ccatttgata aatgattaca
     7261 tcaaatgtct ttagatacct aaagctttgc tatcatactc gtttcatttc ccttatgcaa
     7321 gtaatttttg ttttttatat tgacaataat ttgattcga
//
LOCUS       DANLXF010000119         7357 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.119, whole genome shotgun sequence.
ACCESSION   DANLXF010000119 DANLXF010000000
VERSION     DANLXF010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7357
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.119"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            39..1466
                     /gene="hldE"
                     /locus_tag="PXD66_001616"
     CDS             39..1466
                     /gene="hldE"
                     /locus_tag="PXD66_001616"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HDL6319903.1"
                     /translation="MMMQYSPKFNNAKVLVLGDVMLDRYWFGATNRISPEAPVPVVKV
                     QDIEERAGGAANVAMNIASLGVPVTLHGLIGDDDAGRALDKLLNSHQIQNHCVVINSH
                     PTITKLRILSRHQQLLRLDFEEGFHNVDSTALLAKLSQEITAYGALILSDYGKGTLES
                     VQQMIQIARNAKVPVLIDPKGTDFERYRGATLLTPNMSEFEAVVGHCKDEDEIVSKGL
                     KLIADYDLSALLVTRSEKGMTLLRPNHEPYHLPTQAKEVYDVTGAGDTVISVLATAIA
                     DGRAYEEACYLANAAAGVVVGKLGTSTVSPVELENAIHQRTATGFGVVSEAELKAIVE
                     EAKKRGEKIVMTNGCFDILHPGHVSYLENARKLGDRLIVAVNTDASVKRLKGEARPIN
                     DLAARMAVLAGLASVDWVVPFDEDTPQRLISEILPDLLVKGGDYKPEEIAGSQEVWAN
                     GGDVRVLNFENGCSTTNVIKKIQAL"
     gene            1480..2034
                     /gene="pyrR"
                     /locus_tag="PXD66_001617"
     CDS             1480..2034
                     /gene="pyrR"
                     /locus_tag="PXD66_001617"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDL6319904.1"
                     /translation="MAREKIIIDTEQFQRTISRISHQIIEKHGDLQDVVIVGIKRRGA
                     EIAELIQKRIEDLAQTRLPYMALDITFYRDDLDLIYQDPVFMGADNQLNLNGKKVILV
                     DDVLFTGRTIRAALDALLDFGRAARIELVIFVDRGHRELPIRADYVGKNIPTAKNEQI
                     QVQTLSYDGINQVVLVPAANKESA"
     gene            2107..3057
                     /locus_tag="PXD66_001618"
     CDS             2107..3057
                     /locus_tag="PXD66_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL6319905.1"
                     /translation="MKMKLVKSLFAVAVATMSLTQTAHAFEERVVALVNDTPIMQSQV
                     QRVLGKKKATETAQRAAIDQVIDDMLVQQAMKEAGVKVSPAAVNQAVENVAIQNGITY
                     GQLLDALDYQGITLEQFKRNIAQQMAMEQVRHISISKSIQVAPQQVQALAKELMAKDK
                     AAGKLKTVSGKEHRISHILLKTNPILNDAQAKAKLSSLTTDINSGKISFEEAAKTHSV
                     DYASGADGGDLGFNFLEIYDPAFANVASKIKPNQISAPFKSQFGWHILKVTDTRNGDR
                     TEDAYHQRAYQQLVNKQAEEASKDWVKALRKTANIQYVGQ"
     gene            3119..3985
                     /gene="rsmA"
                     /locus_tag="PXD66_001619"
     CDS             3119..3985
                     /gene="rsmA"
                     /locus_tag="PXD66_001619"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDL6319906.1"
                     /translation="MSSTNSKKHLGHTARKRFGQNFLHDMNIIHSIVSAINPKNGQFL
                     LEIGPGLGALTEPVAELVDKLTVVEIDRDLAERLRHHPFLNQKLTIIEQDALRFNFRD
                     YFNQLNLDEDSVRVFGNLPYNISTPLIFHLLTFHDLIQDMHFMLQKEVVKRLCAAPNS
                     KAYGRLTIMAQYYCQVIPVLEVPPTAFKPAPKVDSAVVRLVPYNQLPYPAKDVYWLNR
                     VTTQAFNQRRKTLRNALSTLFSAEQLDALGVDLTARAENLTIADYVRLANWLHDNPPA
                     IDTTEELVDEDC"
     gene            complement(4096..4725)
                     /gene="upp"
                     /locus_tag="PXD66_001620"
     CDS             complement(4096..4725)
                     /gene="upp"
                     /locus_tag="PXD66_001620"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDL6319907.1"
                     /translation="MKIVEVKHPLVKHKLGLMREAEISTKDFRELANEIGSLLTYEAT
                     KDLEMESVEINGWSGEKIAVERIKGKKVTVVPILRAGLGMMDGVLEHIPSARISVVGI
                     YRDEETLKPVPYFSKLANDVDERLAIITDPMLATGGSMVATIDLLKKAGCKQIKVLVL
                     VAAPEGIKVLEAAHPDVELYTASIDSHLNENGYIIPGLGDAGDKIFGTK"
     gene            complement(4750..6435)
                     /gene="fadD"
                     /locus_tag="PXD66_001621"
     CDS             complement(4750..6435)
                     /gene="fadD"
                     /locus_tag="PXD66_001621"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HDL6319908.1"
                     /translation="MEKIWLNNYPPYSPKTIETDQYESLVEMFEAAIQRHPDIPAYIN
                     MGQVLTFRKLEERSRSFAAYLQNELRLKKGDRIALMMPNLLQYPIALFGALRAGLVVV
                     NVNPLYTPRELEHQLNDSGAKAIVIVSNFAATLEKIVFNTEIKHVILTRMGDQLSFGK
                     RTLVNFVVKYIKKLVPKYKLPHAVSFREALSIGKQRQYVKPNIVSQDLAFLQYTGGTT
                     GVAKGAMLTHQNLIANMMQAKWIVEPLLGNSANMIGLVPLPLYHVLALSMNCLLFIEL
                     GLTGLLITNPRDIPGFIKELKKYKVSVITGVNTLFNALLNHESFKEVDFSHLKLSVGG
                     GAAIQSSVAKRWHDLTGVHIIEGYGMTECSPLISATRGDSVEYSGSIGVPVPNTDIRI
                     VDDEGKEVPLGERGELVVKGPQVMQGYWNRPDETAQVLQDGWMATGDIVVMGDDLNLR
                     IVDRKKDMIIVSGFNVYPTEIEEVISQNPKVNEVVAIGVPSEASGESIKIFVTKKDES
                     LTRDELRTYCRQFLTGYKIPRDIEFRDELPKSNVGKILRRVLRDEELAKVNKA"
     gene            complement(6413..6877)
                     /locus_tag="PXD66_001622"
     CDS             complement(6413..6877)
                     /locus_tag="PXD66_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HDL6319909.1"
                     /translation="MMIEQATVVEYQNGVAIVQCYAKSSCGGCVAQSACGSKALSALA
                     GEKIDPRFRIEVDEPLKVGDKVELGLAENILLKSVFLIYGIPLLVLVMTAVGFSQFFA
                     NELIVLFIMLLSTGITFWAVRKLIHKHSKQANFSPIFLGKVLGDYNGKNMAK"
     gene            complement(6874..>7357)
                     /locus_tag="PXD66_001623"
     CDS             complement(6874..>7357)
                     /locus_tag="PXD66_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HDL6319910.1"
                     /translation="HLLLKSQVLNMDNLVLEEFRVLQLYQSPELDRIANVIESLMLPT
                     LTTVKKEEFPKSDGWQVKWLPTGFRLIQQQTVTGATYQVESEYVDSRVYSDGLSTLTI
                     YIMPSQGVSFNEYAWQQGKLTILNQTINDRDIVIIGDVPLQSAKQIMNNIGFKEGAQP
                     "
BASE COUNT         2152 a         1619 c         1470 g         2116 t
ORIGIN      
        1 tggacgcttg cgccatcatt taacagagaa tattaactat gatgatgcaa tactctccaa
       61 aatttaataa tgctaaagtg ctggtgcttg gcgatgtaat gttagaccgt tactggttcg
      121 gggcaaccaa tcgaatttcg cctgaagccc ctgtgccggt ggtgaaagta caagatattg
      181 aagagcgtgc cggcggtgcg gcaaacgtgg cgatgaacat cgccagcctt ggcgtaccgg
      241 taacattaca cggcttaatt ggcgatgatg atgccgggcg tgctttagat aaattactta
      301 attcccacca gatccaaaat cattgtgtgg tgattaattc gcacccaacc atcacaaaat
      361 tgcggattct ttcccgccat cagcaattat tgcggttgga ttttgaagag ggttttcaca
      421 atgtagattc taccgcccta cttgcaaaac tttcgcaaga aattaccgct tacggagcat
      481 taattctctc ggattacggc aaaggcacat tggaatcggt gcaacaaatg atccaaattg
      541 cccgcaatgc taaggtgccg gtgctgatcg acccgaaagg cacagacttt gaacgctatc
      601 gtggggcaac attgcttaca ccaaatatgt cggaatttga agcggtggtt gggcattgta
      661 aagatgagga tgagattgtc agcaaagggt taaaactgat tgccgactat gatttatctg
      721 cactattagt tacccgttcg gaaaaaggaa tgaccttact tcgcccgaac cacgagcctt
      781 atcatctgcc gacccaagcc aaagaggttt atgatgtaac cggtgcaggc gataccgtaa
      841 tcagtgtatt agccactgcg attgccgatg gcagagccta tgaagaagcc tgctatttag
      901 ccaatgctgc cgccggtgta gtcgtaggta aattagggac ttcaaccgtc agcccggttg
      961 agctggaaaa tgcgattcac caacgcactg ccaccggttt tggcgtggtt tctgaagccg
     1021 aattaaaagc gattgtggaa gaagcgaaaa aacgtggcga aaaaatcgtg atgacgaacg
     1081 gttgctttga tattcttcac cccggtcacg tttcctattt ggaaaacgcc cgtaaattag
     1141 gcgaccgatt aattgtagcc gtcaataccg atgcttcggt aaaacgccta aaaggcgaag
     1201 ctcgcccgat taatgattta gctgcccgta tggcagtttt agctggttta gcctctgtag
     1261 attgggttgt gccttttgat gaagatacac cacaacgttt aatcagtgaa atcctacctg
     1321 atttattggt aaaaggtggt gattacaagc cggaagagat tgccggcagc caagaagtat
     1381 gggcaaacgg cggcgatgtg cgagtattga actttgaaaa cggctgttct accactaatg
     1441 tgatcaagaa aattcaagca ctataatggt aataagcgaa tggcgagaga aaaaatcatt
     1501 attgataccg agcaatttca gcgcacaata tctcgtattt cacatcaaat tattgaaaaa
     1561 cacggtgatt tgcaagatgt agtgattgtc ggcatcaaac gtcgaggagc tgaaattgcc
     1621 gagttaatcc aaaaacgcat tgaagattta gctcaaacac gcttgcctta tatggcgtta
     1681 gatattacgt tctaccgtga tgacctagac ttaatttatc aagatcccgt atttatggga
     1741 gccgataatc agctaaatct gaacggtaaa aaagtgattt tggttgatga tgttctgttt
     1801 accggcagaa ccattcgggc ggcacttgat gcactcttag attttggcag agctgccaga
     1861 attgaactgg tgatttttgt cgatagaggt catcgggaat tgcctatccg agccgattat
     1921 gtcggaaaaa atattccaac ggctaaaaac gagcaaattc aggtgcaaac gctctcgtat
     1981 gatgggatta accaagtggt cttagttcct gctgccaata aagaaagtgc ttaaattaag
     2041 tgaacctttt cgctaaactt atctctaagg aaaagttact gcttaattac ctccattagg
     2101 attgttataa aaatgaaatt agtcaaatca ttatttgctg ttgccgttgc aacaatgagt
     2161 cttacccaaa ccgcccacgc atttgaagaa agagtggtgg cgttagtgaa tgatacccct
     2221 attatgcaaa gccaggttca acgtgtgctt ggcaagaaaa aggcgacaga aactgctcaa
     2281 cgtgcggcta ttgatcaagt gattgatgat atgttggtgc aacaggcaat gaaagaagcc
     2341 ggcgttaaag tcagcccagc tgcggtaaac caagcggttg aaaatgtagc aattcaaaac
     2401 ggcattactt acggtcagtt attagatgcg ttagattatc aaggtattac acttgaacaa
     2461 ttcaaacgta atattgctca acaaatggca atggaacaag ttcgccatat cagcattagc
     2521 aaatcgattc aggttgcacc acagcaagtg caagctctgg cgaaagagtt aatggcgaaa
     2581 gataaagcag caggcaaatt gaaaaccgtt tctggcaaag agcatcgcat tagtcatatt
     2641 ttgctcaaaa ccaacccgat tttaaatgat gctcaagcca aagcaaaact tagcagctta
     2701 actactgata ttaactcagg caaaatcagc tttgaagaag cggcaaaaac ccattctgtt
     2761 gattatgcct caggggcaga cggtggagat ttaggtttta acttcttaga aatttacgat
     2821 ccggcatttg cgaatgttgc atcaaaaatc aaaccaaacc aaatttctgc tccgtttaaa
     2881 tcgcaatttg gttggcatat tttaaaagtc accgacactc gtaacggaga cagaaccgaa
     2941 gatgcttacc accaacgtgc ttatcagcaa ctggtgaaca agcaagccga agaagcctct
     3001 aaagattggg taaaagcgct tagaaaaacc gccaatattc aatatgtcgg tcaataatta
     3061 aaatttttaa gaacctcgca taatgcgggg ttcttttctt atttcaaaaa ggataacaat
     3121 gagttctaca aattccaaaa aacatttagg tcataccgct cgtaagcgtt tcgggcaaaa
     3181 ctttttgcac gatatgaaca ttattcacag catagtttca gccattaacc cgaaaaacgg
     3241 acaattttta ttagaaatcg gtccgggttt aggagcatta accgagccgg ttgccgaatt
     3301 ggtagataaa ctcacggtgg ttgaaattga ccgtgattta gcagagcgtt tacgccacca
     3361 cccgttttta aaccaaaaac tcaccattat tgagcaagat gccctacgct ttaatttccg
     3421 tgactatttt aaccaactca acttagacga agactcggtg cgtgtgtttg gtaacttacc
     3481 ttacaacatt tctacgccct tgatattcca cctgctgacc tttcacgact taattcaaga
     3541 tatgcacttt atgttgcaaa aagaggtggt gaaacgttta tgtgccgcgc caaacagtaa
     3601 agcatacggg cgtttaacca ttatggctca atactattgc caagtaattc cggtgctgga
     3661 agtgccgcca accgccttta aaccggcacc aaaagtcgat tctgcggtag tgcgtttagt
     3721 gccttataac caactgcctt atccggcaaa agatgtgtat tggctcaatc gggtcacgac
     3781 tcaagccttt aaccaacgcc gtaaaacctt acgcaatgct ctttcaaccc tgtttagtgc
     3841 ggagcaactg gacgccttag gcgttgattt aaccgcccga gcggaaaacc ttaccattgc
     3901 cgattatgtg cgtttggcta actggctaca cgacaacccg ccggcaattg atacaaccga
     3961 agaattggtt gatgaagatt gctaatcaat aaattacaag cggtcatttt tctaccaaga
     4021 tttgcaaatc tttagtgaaa aatggccgct tgtttaaatc agctttactt gttttgctgt
     4081 tacttagttt tgatgttact tggtgccgaa aattttatcc cctgcatcgc ctaagcctgg
     4141 aatgatatag ccgttttcgt ttaagtgact atcgattgag gcggtgtaga gttcaacatc
     4201 tgggtgggcg gcttctaata ctttaatccc ctctggagcg gcaactaaca ctagcacttt
     4261 aatctgctta cagcctgctt ttttcaggag gtcaatggtt gctaccattg agccaccggt
     4321 tgcaagcatt gggtcggtga taatggctaa acgctcatca acatcgttgg ctagtttgga
     4381 gaaatacggc acgggcttta aggtttcttc gtcacgataa atcccaacca cactaattct
     4441 ggcacttgga atatgctcta acacaccgtc catcatacct aagccagcac gcagaatcgg
     4501 cacaacagtc acttttttgc ctttaattcg ctcaaccgca attttctctc cgctccagcc
     4561 attgatctcc acactttcca tttccagatc tttggttgct tcgtaagtaa gcagactacc
     4621 aatttcattc gctagctcac ggaaatcttt ggtgctgatt tccgcttcac gcattaagcc
     4681 caatttatgc tttacaagtg ggtgttttac ttcaacaatt ttcattttaa gctccttaat
     4741 tagtgtaatt taagccttat tcacctttgc taattcttcg tctcttaaca ctcggcgaag
     4801 aattttgccg acattacttt ttggtaattc gtcacggaat tcgatgtctc ttggaatttt
     4861 gtagccggtt aagaattgac gacaataggt tctcaattca tctcgagtta aactttcatc
     4921 ttttttggtt acaaaaattt taatgctttc gccggaagcc tcactcggca caccaatcgc
     4981 cactacttca ttcacttttg ggttttggga aatcacttct tcaatttcgg ttgggtacac
     5041 attaaaaccg gagacaataa tcatatcttt tttacgatca acaatgcgta agttcagatc
     5101 atcccccatt accacaatgt caccggttgc catccaacca tcttgtagca cttgtgccgt
     5161 ttcatcagga cggttccaat agccttgcat tacttgcggc cctttcacca ccagctcgcc
     5221 ccgttcgccg agtggtactt ctttgccttc atcatccaca atacgaatgt cagtatttgg
     5281 cactggcacg ccaatagagc cggaatattc caccgaatcg ccacgggttg cactaatcag
     5341 cggagaacat tctgtcattc cataaccttc gataatatgt acgccggtta aatcgtgcca
     5401 gcgttttgcc acgctagatt ggatcgctgc accgccacct acacttaatt ttaggtgtga
     5461 aaaatcaacc tctttaaaac tttcgtgatt aagtaatgca ttaaacaatg tatttacccc
     5521 agtaatcact gaaactttgt acttttttaa ttctttaata aagcctggaa tatcgcgcgg
     5581 attagtaatt aataatccgg taagtcctaa ttcgataaag agcaagcaat tcatcgaaag
     5641 tgctaagacg tgatacagcg gtaaaggtac caatccaatc atattggcag aattacccaa
     5701 caatggctca acaatccatt ttgcctgcat catattagca attaagtttt gatgagtcag
     5761 cattgcccct ttcgctacac ctgtagtacc gccagtgtat tgtaaaaagg cgagatcttg
     5821 gctgacaata ttcggtttta catattgacg ctgtttgcca atgcttaagg cttcacggaa
     5881 gctgaccgca tgtggcaatt tatatttcgg cactaatttt ttgatgtatt tcaccacaaa
     5941 attgactaag gtgcgtttgc cgaatgaaag ttggtcgccc attcgggtca aaataacgtg
     6001 cttaatctca gtattaaaga caattttttc taaggtagcc gcgaagttcg agactatcac
     6061 aatcgctttc gcaccactgt catttaattg atgttctaat tcacgcggcg tataaagcgg
     6121 gttgacattc accaccacaa gcccggcacg taatgcgcca aataaagcga tcggatattg
     6181 caataaattc ggcatcatca atgcgattcg atcacctttt ttcaggcgta attcattttg
     6241 caaataagcg gcaaacgagc ggctacgttc ttccaattta cggaacgtta aaacctgccc
     6301 catattaata taagctggaa tatcagggtg gcgttgtatc gctgcttcaa acatttccac
     6361 taaagattca tattgatctg tttcaatcgt ttttggtgaa taaggcggat aattatttag
     6421 ccatattttt tccattatag tctcctaaaa ccttacctaa aaagataggt gaaaaatttg
     6481 cttgtttgct gtgtttatga atcaattttc ttacagccca gaatgttata cctgttgaaa
     6541 gcaacattat aaataaaaca ataagctcat ttgcaaaaaa ttgcgaaaat ccgaccgctg
     6601 tcatcacaag aaccagcaga ggaatgccat aaattaaaaa cacgcttttg agcaagatat
     6661 tttcagctaa ccctagttca accttatcgc ctacttttag gggttcatct acctcaatac
     6721 gaaaacgagg gtcaattttt tcacctgcta atgctgataa tgctttgctg ccacaagcag
     6781 actgagctac acagccacca cagctacttt tagcataaca ttgtacaata gccacaccat
     6841 tctgatattc caccaccgta gcctgttcga tcatcatggc tgagctcctt ccttaaaacc
     6901 aatattgttc ataatttgct tagccgattg caacggcaca tcaccaataa tcacaatatc
     6961 tctatcatta atcgtttgat ttaaaatcgt taatttacct tgttgccaag cgtactcatt
     7021 aaaactcacg ccttggctag gcataatata aatagttaat gtagataaac catcactata
     7081 tactctgcta tctacatact cactttccac ttgatatgtc gcacctgtga ctgtttgttg
     7141 ctgaattaac ctaaaccctg tcggcaacca cttcacctgc caaccatcag acttaggaaa
     7201 ttcttctttt ttcactgtcg ttaatgttgg taacattagt gattctatta catttgcaat
     7261 cctatccaat tctggagatt gataaagttg caaaactctg aattcttcta ataccaaatt
     7321 atccatattc aagacttggc ttttaagtaa taaatgt
//
LOCUS       DANLXF010000120         7298 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.120, whole genome shotgun sequence.
ACCESSION   DANLXF010000120 DANLXF010000000
VERSION     DANLXF010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7298
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.120"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..514
                     /locus_tag="PXD66_001624"
     CDS             <1..514
                     /locus_tag="PXD66_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal replication protein"
                     /protein_id="HDL6319911.1"
                     /translation="KKLFIAQIKQNLSELNVFSTDNIFLNSPYFSQQTGLVSVFIAEI
                     EKTVELLLNQTEVLYSEFYAEKLVKQVDALKNAVEKIQSKPESAQFHSSYQFSPNIHR
                     LAPNKRLQEYRKALRALNEKISWLVEQNLNTQNEATKQTLQNQITETEYRKMKCLKAI
                     EDLEQELLFK"
     gene            complement(518..591)
                     /locus_tag="PXD66_001625"
     tRNA            complement(518..591)
                     /locus_tag="PXD66_001625"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(557..559),aa:Cys,seq:gca)
     gene            complement(607..682)
                     /locus_tag="PXD66_001626"
     tRNA            complement(607..682)
                     /locus_tag="PXD66_001626"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(647..649),aa:Gly,seq:gcc)
     gene            complement(892..2316)
                     /gene="lpdA"
                     /locus_tag="PXD66_001627"
     CDS             complement(892..2316)
                     /gene="lpdA"
                     /locus_tag="PXD66_001627"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011272714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDL6319912.1"
                     /translation="MSKEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYSTLGGI
                     CLNVGCIPSKALLHVAKVIEEAKNAVHNGVTFGEPTIDLDQVRAGKEAVVSKLTGGLA
                     GMAKARKVTVVEGLAAFTGPNTLVARDRDGNPTTITFDNAIIAAGSRPIQLPFIPHED
                     PRVWDSTDALKLKEVPKKLLIMGGGIIGLEMGTVYNALGSEVEVVEMFDQVIPAADKD
                     VVAIYTKQIEKKFKLMLETKVTAVEAKDDGIYVSMEGKACNDTKRYDAVLVAIGRVPN
                     GKLIDAGKAGVEVDERGFIRVDKQMRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGMKHYFDPKVIPSIAYTEPEVAWVGKTEKECKQEGLNYEVAKFPWAASGRAIASEC
                     SEGMTKLIFDKDTHRVLGGAIVGSNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNAKAKKR"
     gene            complement(2516..4426)
                     /gene="aceF"
                     /locus_tag="PXD66_001628"
     CDS             complement(2516..4426)
                     /gene="aceF"
                     /locus_tag="PXD66_001628"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HDL6319913.1"
                     /translation="MSKQINVPDIGSDEVSVTEVMVKVGDTISVDQSIINVEGDKASM
                     EVPAPEAGVVKEVLVKVGDKVSTGSPMLILESADAAPAQEAPKAEAAPAAPVATTSAV
                     VEVNVPDIGGDEVNVTEIMVKVGDSVEVDQSIINVEGDKASMEVPAPVAGVVKEILIN
                     VGDKVSTGKLIMKFETASAATAQSTPVAEAAPAQASASAIKDVNVPDIGGDEVNVTEI
                     MVKVGDSVSEEQSLITVEGDKASMEVPAPFAGVVKEILVKSGDKVSTGSLIMKFEVAG
                     AAPAPAAAQAASAPAPAPQAAAPAKADAAPAGQNQSGLSQEQVVASAGYAHATPVIRR
                     LAREFGVNLDKVKGTGRKGRIVKEDIQAYVKTAVQVFEKQGGTAAAATGAANGAGLGL
                     LPWPKVDFSKFGEVEEVELSRINKISGANLHRNWVMIPHVTHFDRTDITELENFRKEQ
                     NKEAEKRKLDVKITPVVFIMKAVASALEAFPRFNSSISEDAQRLTLKKYINIGVAVDT
                     PNGLVVPVFKNVNKKGIIELSRELMEVSKKAREGKLTASDMQGGCFTISSLGGIGTTH
                     FTPIVNAPEVAILGVSKSEMQPIWNGKEFEPRLMLPLSLSFDHRVIDGADGARFLSYI
                     NSVLADIRRLVM"
     gene            complement(4630..7287)
                     /gene="aceE"
                     /locus_tag="PXD66_001629"
     CDS             complement(4630..7287)
                     /gene="aceE"
                     /locus_tag="PXD66_001629"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005628834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HDL6319914.1"
                     /translation="MSENLRNDVDPIETQDWLASLDSLIREEGLERAQFIIEQVISQA
                     RAGGVALPTGVTTDYVNTIPVSEQPAYPGDHKIERRIRSAVRWNAIAAVLRSQKKDLD
                     LGGHISTFQSAATMYEVCFNHFFKAPTEKDGGDLVFFQGHAAPGMYGRAFLEGRITAE
                     QMDNFRQEAFTDGLSSYPHPKLMPEFWQFSTVSMGLGPVNAIYQARFLKYLENRGLKD
                     TSAQKVYAFLGDGEMDEIESKGALSFAGREKLNNLIFTISCNLQRLDGPVNGNGKIVQ
                     ELEALFTGCGWEVIKVLWGSGWDKLLEKDTSGKLKQLMMEVLDGDYLTFKSKNGAYVR
                     EHFFGRYPETAALVADMTDDEIWALRRGAHDSEKLYAAYAKAQKSDKPVVILAHQVKG
                     YRIPEAESKNTAHQSKKMSLDSLKGFRDYFELPLTDEQVESLEYVRFPEGSEEYNYLH
                     GQRKALNGYVPVRKPKFTVDFKVPELSEFQALLDEQPRGISTTMAFSRVLNTLLKDKN
                     IGQQIVPIIADEARTFGMEGLFRQIGIYNPHGQNYVPSDRDLVAYYREAKDGQVLQEG
                     INELGATASWVAAATSYSVSNLPMIPFFIYYSMFGFQRVGDMMWLAGDQLARGFMIGG
                     TSGRTTLNGEGLQHEDGHSHIQAGVIPNCVTYDPAFAFEVAVIVQDGIRRMYGENQED
                     IFYYITTLNEITEQPAMPAGAEEGIRKGLYKFETVEGKGKGHVQLLSSGAIMRHVRAA
                     AQILANDYGITADVFSAPSFNELGRDGADVARWNLLHPTETPRVPYVAQVLQDLPTVA
                     STDYMKMYAEQIRAYVPSKHYHVLGTDGFGRSDSRANLREHFEVDERYVVVAALAQLA
                     KEGTVENKVVAEAITKFGLNVDRINPLYA"
BASE COUNT         2061 a         1664 c         1373 g         2200 t
ORIGIN      
        1 aaaaaaactt tttatcgccc aaataaaaca aaacctctcg gaattaaatg tattttcaac
       61 ggataatatt tttctgaatt caccctattt ctcacagcaa accggtttgg tgagtgtgtt
      121 cattgctgaa attgaaaaaa cagttgagct attattgaac caaactgagg tgttatatag
      181 tgaattttac gctgaaaaat tggttaaaca agttgatgca ttaaagaatg ccgtagaaaa
      241 aatccaaagt aagccagaat cagctcaatt ccattcttcc tatcaatttt ctccaaatat
      301 tcatcgttta gcgcccaaca aacgcttaca agaataccgt aaagcgttac gtgcattgaa
      361 cgagaaaatt agctggttag ttgagcaaaa tttaaatacg caaaatgaag caactaaaca
      421 aaccctgcaa aatcaaatta cagagacaga atatcgcaaa atgaaatgtt taaaagcaat
      481 tgaagattta gaacaagagt tgctatttaa atagtgatgg aggcgtgtcc cggagtcgaa
      541 ccgagctaca tggatttgca atccagtgca taaccgcttt gctaacacgc cataagattt
      601 ggaaattgga gcgggaaacg aggctcgaac tcgcgacccc gaccttggca aggtcgtgct
      661 ctaccaactg agctattccc gcatctgaat tcccacaaca ttgcgctgtg gaacgaggtg
      721 cattttacgg atttatcatt ttatgtcaat gctaaattta acattctaga ttcaaatgct
      781 gaaaaaaaac tcactattca atagtttagt tttaaaaata atcaagcggt tgtattttgc
      841 aaaaaattta caaaatacaa ccgcttattt tttagagatt aaataatctg attaacgctt
      901 tttggctttc gcatttggta agtctgtaat tgaaccttca aatacttctg ccgctaatcc
      961 taccgattcg tgtaacgttg ggtgagcatg aatggttaat gcgatatctt cagcatcaca
     1021 gcccatttcg attgcaagac cgatttcgcc taataattcg ccaccattgc taccaacaat
     1081 cgcaccacct aatacacggt gagtatcttt atcaaaaatt aacttggtca taccttctga
     1141 gcattcagaa gcaatcgcac gacctgaagc agcccaaggg aatttcgcca cttcgtagtt
     1201 caagccttct tgtttacact ctttctcagt tttaccaacc caagccactt ctggttcagt
     1261 ataagcgatg gacggaatga ctttcggatc gaagtaatgt ttcattcctg caatcacttc
     1321 tgctgccacg tgtccctcgt gtacaccttt gtgagctaac atcggctggc cgacaatatc
     1381 accaatagca aagatgtgtg gcacattggt acgcatttgt ttatctacac gaataaagcc
     1441 acgctcatct acttccactc cagctttacc ggcatcaatt aatttaccgt ttggcacacg
     1501 tccgatagcg actaataccg catcataacg tttagtatcg ttgcaagcct taccttccat
     1561 tgatacatag ataccgtcat ctttagcttc tactgcggtt actttggttt caagcattag
     1621 cttgaatttt ttctcgattt gtttggtgta aatcgctaca acatctttat ctgcagcagg
     1681 aattacttgg tcgaacattt caaccacttc cacttctgaa ccaagcgcgt tatataccgt
     1741 tcccatttct aaaccgataa taccaccacc cataataagg agttttttcg gtacttcttt
     1801 taatttaaga gcatcggttg aatcccaaac tcgtgggtct tcgtgtggaa taaatggtag
     1861 ttgaatcgga cgagaacctg ctgcgataat cgcattatcg aacgtgatag ttgtaggatt
     1921 gccgtcacga tcacgagcga ctaacgtatt cggaccagta aatgcagcca agccttctac
     1981 tactgtcact ttacgagcct tagccattcc tgctaagcca ccggttaatt ttgaaacaac
     2041 ggcttcttta ccagcacgta cctgatctaa atcaatcgtt ggctcaccaa aggtcacacc
     2101 gttgtgtact gcatttttag cttcttcaat tacttttgca acgtgtaata atgctttaga
     2161 agggatacaa cctacgttta agcaaatacc acctaaggtt gaataacgct caacaattac
     2221 tgtttctaag cctaagtcgg cacaacggaa tgccgctgag taacctgctg gacctgcacc
     2281 aagtacgaca acttgggttt taatttcttt gctcatagtt tttcctcttt ttattccccc
     2341 cctttagcaa agaggggtaa gggagatttg gcagaatata aagcgaagtg agagagcaat
     2401 caatcaaaca caaatttcta ccaaataatt tattctcttc gtaaattacg tctatcaaat
     2461 ctccccctac ccctcttttc taaagaggga cttttttagg aggagcttta ccgaattaca
     2521 tcactaaacg acgaatatct gccaacacgc tattaatata gcttaagaat ctcgcaccat
     2581 cagcaccatc gatcacacgg tggtcgaatg ataatgaaag cggaagcatt aagcgtggtt
     2641 caaactcttt gccattccaa atcggttgca tttcagattt agacacacct aaaatagcca
     2701 cttccggtgc attcacgatt ggcgtaaagt gcgttgtacc aataccgcct aaacttgaaa
     2761 ttgtgaaaca gccaccttgc atatcagatg ctgtcaattt accttcacgg gcttttttcg
     2821 agacttccat taattcgcga gaaagttcga taatgccttt tttgttcaca tttttgaaca
     2881 ccggcaccac taaaccgttt ggcgtatcaa ccgcaacccc gatattgatg tattttttca
     2941 aggttaaacg ttgtgcatct tcagaaattg agctgttgaa acgagggaag gcttctaatg
     3001 cacttgccac tgctttcata atgaagacca caggggtaat tttcacgtct aatttacgtt
     3061 tttccgcttc tttattttgc tctttacgga agttttctaa ctcggtaatg tctgtgcgat
     3121 cgaaatgtgt aacgtgagga atcattaccc agttacggtg taagttcgca ccggaaatct
     3181 tattgatacg gcttaattca acttcttcaa cttcaccaaa cttgctgaaa tctactttcg
     3241 gccacggtaa taaacctaag cctgcaccgt ttgccgcacc tgttgctgcc gctgctgttc
     3301 cgccttgttt ttcaaatact tgaacagccg ttttaacgta tgcttgaata tcttctttaa
     3361 cgatacggcc tttacgacct gtacctttta ctttgtctag gtttacacca aactcacgag
     3421 ctaaacggcg aattaccggt gttgcgtgtg catagccagc tgaagccacc acttgttctt
     3481 ggcttaaacc tgattggttt tgacctgccg gagctgcgtc agctttcgcc ggagctgctg
     3541 cttgtggagc aggagcagga gctgaagccg cttgagcagc cgccggagcc ggtgccgcac
     3601 ccgctacttc aaatttcata attaatgaac cggttgaaac tttatcacct gatttcacta
     3661 aaatttcttt caccacaccg gcaaatggag ccggaacttc cattgaagct ttatcacctt
     3721 caaccgtgat taaagattgt tcttctgaaa cagaatcacc cactttaacc ataatttcag
     3781 ttacgtttac ttcatcacca ccgatgtccg gtacatttac atctttaatc gctgatgccg
     3841 aagcttgagc tggagcagct tccgcaaccg gtgtagattg tgctgtagct gcggaagccg
     3901 tttcaaattt cataatcaat ttaccagttg aaactttgtc acccacattg attaagattt
     3961 cttttactac gccggcaacc ggtgcaggca cttccattga ggctttatca ccttcaacgt
     4021 tgatgataga ttgatccact tcaacgctat cacctacttt aaccataatt tcggttacgt
     4081 taacttcatc gccaccgata tcaggtacat ttacttcaac aacggctgaa gttgttgcta
     4141 ctggagcagc aggagctgct tctgcttttg gtgcttcttg agcaggagca gcatctgctg
     4201 actctaaaat taacattggt gaaccggttg aaactttatc acccactttc actaaaactt
     4261 cttttaccac acctgcttca ggagccggca cttccataga ggctttatcg ccttctacgt
     4321 taataattga ttgatctact gaaatagtat caccaacttt taccatcact tcagtcacac
     4381 tgacttcatc cgaaccgatg tccggcacat taatttgttt tgacattttg aaatccttct
     4441 tgagttaccc cctctcccat tgtgggaggg acagattttt ctttgtaaga agaaaaatca
     4501 gggagaaggg aatgcaagcg gttgattttc tcaacatttt tgcaaataaa actaataccc
     4561 tctctgctaa aagtttctaa acgaaacctt tagctgtctc tctcccatca agggagagag
     4621 tattagaaat tacgcatata atgggttaat acgatctaca tttaagccga atttagtaat
     4681 agcttccgca accactttgt tttcaactgt accttctttc gctaattgag cgagggctgc
     4741 aactactaca taacgctcat caacttcgaa gtgttcacgt aagtttgcac ggctgtctga
     4801 acgaccgaaa ccgtctgtac ctaacacgtg atagtgttta cttggtacat aagcacggat
     4861 ttgctctgcg tacattttca tatagtcggt tgaagcaaca gtcggtaaat cttgtaacac
     4921 ttgagcaacg tacggtacac gtggtgtttc agttgggtgt aataagttcc aacgagccac
     4981 atctgctcca tcacgaccta attcattgaa tgatggtgct gagaatacat ctgctgtaat
     5041 gccgtagtca ttcgcgagaa tttgtgcagc tgcacgcacg tgacgcataa ttgcacctga
     5101 gcttaataat tgtacgtgac ctttaccttt accctcaacg gtttcaaatt tatataaacc
     5161 tttacggata ccttcttccg cacctgccgg cattgccggc tgttcggtaa tttcatttaa
     5221 ggtagtaatg tagtagaaga tatcttcttg gttttcacca tacatacgac gaataccgtc
     5281 ttgaacaata accgcaacct caaaggcaaa tgcagggtca taagtgacgc agttcgggat
     5341 tacacctgct tgaatatggc tatgaccatc ttcgtgttgt aaaccttcac cgtttaatgt
     5401 tgtacgacct gaagtaccac caatcataaa gccacgagct aattggtcac cggctaacca
     5461 catcatatcg cctacacgtt ggaaaccaaa cattgagtag tagatgaaga atgggatcat
     5521 cggtaagttg ctaacagaat aagaagtcgc tgctgccacc caagaggctg ttgcacctaa
     5581 ctcattgata ccttcttgta acacttggcc atcttttgct tcacggtagt aagctactaa
     5641 atcacggtct gacggtacat agttttgacc atgtgggtta taaataccga tttgacggaa
     5701 taaaccttcc ataccgaaag tacgggcttc gtccgcaata ataggtacga tttgctgacc
     5761 gatattttta tctttcaata aagtattcaa tacacgagag aacgccattg tggttgaaat
     5821 accacgtggt tgctcatcca ataatgcttg gaattcgctt aattcaggca ctttgaaatc
     5881 aaccgtaaat ttcggtttac gtacaggcac ataaccgttt aaggctttac gttgtccgtg
     5941 taagtagttg tactcttcag aaccttcagg gaatcttaca tactctaggc tttctacttg
     6001 ctcatcggtt aatggtagct cgaagtagtc acggaagcct tttaagctgt ctaatgacat
     6061 ttttttcgat tggtgagcag tatttttact ttccgcttca ggaatacggt agcctttcac
     6121 ttggtgagct aaaattacaa ctggtttgtc cgatttttgt gcttttgcat aggctgcata
     6181 aagtttttca gaatcgtgtg caccacggcg taatgcccaa atttcatcgt ctgtcatatc
     6241 agcaactaat gctgcagttt cagggtaacg accgaagaag tgttcacgaa cgtaagcacc
     6301 atttttggat ttgaaggtta aatagtcacc atcaagcact tccatcatta actgtttaag
     6361 tttgcctgaa gtatcttttt caagcagttt atcccaacca ctaccccata acactttaat
     6421 cacttcccag ccacaaccgg taaagagtgc ttctaattct tgcacgattt tgccgttacc
     6481 gttaaccgga ccgtctaaac gttgtaagtt acagctaata gtgaagatta agttatttaa
     6541 tttttcacga ccggcgaatg ataatgcacc tttagattcg atttcatcca tttcgccatc
     6601 gcctaagaat gcatatactt tttgagccga agtatctttt agaccacggt tttctaaata
     6661 ttttaagaaa cgagcttggt aaatagcatt taccggtcct aaacccatag atacggtaga
     6721 gaattgccaa aattcaggca ttaatttagg gtgtgggtaa gaagataaac cgtctgtaaa
     6781 tgcttcttga cggaagttat ccatttgttc tgcggtaata cgaccttcta agaacgcacg
     6841 accatacata cccggtgctg cgtggccttg gaagaacact aaatcaccac catctttttc
     6901 ggttggtgct ttgaaaaagt ggttaaaaca cacttcatac atagttgccg cagattggaa
     6961 agttgaaata tgaccaccta agtcaagatc tttcttctgg ctacgaagta ctgccgcaat
     7021 cgcattccaa cgtaccgcag aacgaatacg acgttcgatt ttatgatcac ccggataagc
     7081 tggctgctcg gaaacaggga tagtattgac ataatctgtt gttacgcctg ttggtaatgc
     7141 cacgccacct gcacgagctt ggctaataac ttgttcaatg ataaattgag cgcgctctaa
     7201 accttcttca cgaattaatg aatcgagtga tgctaaccaa tcctgagttt cgattggatc
     7261 tacgtcattt cttaagttct ctgacatagt attcttcc
//
LOCUS       DANLXF010000121         7174 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.121, whole genome shotgun sequence.
ACCESSION   DANLXF010000121 DANLXF010000000
VERSION     DANLXF010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7174
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.121"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(76..3609)
                     /gene="mfd"
                     /locus_tag="PXD66_001630"
     CDS             complement(76..3609)
                     /gene="mfd"
                     /locus_tag="PXD66_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDL6319915.1"
                     /translation="MSLISLNLPKSDGNFQDHQTLGNLVGHSDTLAISQAARAFYGLT
                     VVVTPDTRTALRLEKALPQFAGLPVQLFPDWETLPYDNFSPHQDIISARLSALFELQQ
                     GRQQILLLPINTLMQKVCPPNYLANNVLLIKKGDRFSIQKLRLQLENAGYRAVEQVLE
                     YGEYAVRGSILDLFPMGAEEPFRLDFFDDEIDSIRTFDVDNQRTKTEIAEINLLPAHE
                     FPTDSNGIEHFRAKFREKFGEIRREPEHIYQQVSKGILNAGIEYWQPLFFEEMASLFD
                     YFPAQTLFITFADIQQKAEQFHQDTQNRYESHRVDPMRPLLPPSDLWFAIDEVNRWLK
                     GYPRLTITSEKIRKSAAKMNANVANLPDIAIQSSAKEPFSAFQQFRQKFDGNILFSVE
                     SEGRRETLLDLLAPLGVKPKQVNSLDEAQGLPFSLIISPLDQGFVVETGGHGTRPYDD
                     ELNTNGENDNNYVGAGPVPARNLAIICETDLLGERVQTRREKNRKAVNPDTLIRNLAE
                     LKIGQAVVHLENGVGRYGGLTVLDAGGIKAEYLVLHYANDAKLYVPVASLHLISRYIG
                     GADETAPLHKLGSEAWAKTRQKAAEKIRDVAAELLDVYAKRESQTGFAFAYDKAEFDQ
                     FSATFPYEETDDQKIAINAVISDMCQAKAMDRLVCGDVGFGKTEVAIRATFLAVMNHK
                     QVVILAPTTLLAQQHYENFKDRFANYPVNVEVLSRFKTAKEQKTILQNVAEGKVDILV
                     GTHKLLQDDVAFRDLGLLVIDEEHRFGVRQKEKIKQLRANVDILTLTATPIPRTLNMA
                     LNGMRDLSIIASPPARRLLIKTFVRQSDEMVIREAILREILRGGQVYYLHNDVATIEN
                     CAEKLAELVPEARIVIGHGQMRERELERVMSDFYHQRFNLLVCSTIIETGIDVPTANT
                     IIIERADKFGLAQLHQLRGRVGRSHHQAYAYMLTPHPKTLTKDAEQRLEAMSTIDNLG
                     AGFALATHDLEIRGAGELLGSEQSGQIESIGFSLYMDLLENAVKALQEGREPSLDEIT
                     QHQAEIELRVPALLPDDYLPDVNMRLSFYKRIASSENLQSLNDLKVELIDRFGLLPEA
                     TKNLFAITQLRLSAQTLGIKKIDAGIHGGYLEFKPTAQPDPMKFIALIQKQPAVYSFE
                     GAVKFKFRLALEDNRKRLEFVENLLREVVGG"
     gene            3770..4987
                     /locus_tag="PXD66_001631"
     CDS             3770..4987
                     /locus_tag="PXD66_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter"
                     /protein_id="HDL6319916.1"
                     /translation="MKNKILGSALMIAGTTIGAGMLAMPLTSAGMGFGATALLLVGLW
                     ALLAYTGLLFMEVYQTAPQKDVGVASLAEQYFGLIGRVLATFSLLVLLYALLAAYITG
                     GGSLLAGVMPEMADADMKLKISILIFTAVLGTFVVVGVKSVDGLTRVLFLGKIVAFIA
                     VLLMMLPKAKLENLTAIPLDNLLIISAIPIFFTAFGFHVIMGTINSYLDADIGKIRKS
                     IYIGTAIPLVAYLLWQLATHGVLSQNEFTTILTQDPTLNGLVKATSQITGSTILGEMV
                     RLFSVLALITSFLGVSMGIFEGVGDLLKRLNLPTNRLWLTIATFTPPILFALFYPNGF
                     IKALGYAGLLFAFYGMILPIGLAWKARRQYPNLPYRVIGGNLALVVALIAGIIIMVIP
                     FLIQAGYLPQAVG"
     gene            complement(5067..5573)
                     /gene="hutX"
                     /locus_tag="PXD66_001632"
     CDS             complement(5067..5573)
                     /gene="hutX"
                     /locus_tag="PXD66_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HDL6319917.1"
                     /translation="MISIEQQTALRNELAENPGQILEMLAAKYQCTLEEVMLNLPAEM
                     LKVTEGERFAEVLQQIHEWDDAITLISHTEDTIIEFVGKLPSGSISRGFYNFEHKEGG
                     GLQGHLRYENCAKIYLLDRPFMGKRTVSLIFINKKGNAMFKIFVGREKIGGALKEHQI
                     KALYQLIG"
     gene            complement(5628..>7174)
                     /locus_tag="PXD66_001633"
     CDS             complement(5628..>7174)
                     /locus_tag="PXD66_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005715618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDL6319918.1"
                     /translation="WREAEDRSKRDTVNVFYEYFPESKWISLAKIDADYQDTETSAYN
                     YEGTRAEDATDWSPAKQRKPSDNNIRIFNTELKRLNFRLDSQALEFGKWTHQLSFKAS
                     TAERDFDVLHKDSVYLSGVWVNSPDSTMMYPIKTKQHTFSLHDKIDLTHDWIATLGIR
                     YDWAKYEQQELNGLVCRNCVKTDNAKFNQLTWVTGLEKHLTEAWKVGYNIGTGFRIPN
                     ASEMYFDYRDNAAGAWMSNPNLKAERSLSQNLSLQGSGNVGLLSVNFHHTKYKDFLWE
                     QETWDVYQAYGREFWRPVQQMQNIDSAKIYGLEVSGKWNLNSAMPIPEGWKLFGAFGY
                     SKGSMSNGADLLSIQPIKAVVGLDYEQPEGKWGVFSRFTFLGAKKAKDAKYLKTLPEK
                     CLKEDRTPNPYYPYWGDEYDIRCTEYSHETGLDTWKHLNAKAFVVDLYGFYKPTENIT
                     LRAGIYNLFNRKYHTWDTLRGLNTTGGIVNSVGVRENATYGGYPGLQRYYEPGRNFSA
                     NFEYRF"
BASE COUNT         1860 a         1654 c         1528 g         2132 t
ORIGIN      
        1 tcgcaataaa caaaaacgat ttttacgata cagatcgcaa aaatcgtttt tcaagattga
       61 caaccttagg aggctttatc cgcccacaac ctcccttagc aaattttcca caaattctaa
      121 ccgcttgcgg ttgtcttcga gtgctaagcg gaatttgaac ttcaccgccc cttcaaaact
      181 gtacaccgct ggttgttttt ggattaaggc gataaacttc attgggtcgg gctgggctgt
      241 cggtttaaat tcaagataac cgccgtgaat gccggcatcg attttcttaa tcccgagcgt
      301 ttgtgccgac aagcgaagtt gggttatcgc aaacaggttt ttggttgcct ctgggagcaa
      361 gccgaagcga tcgatcagct ccactttcaa gtcgttcagg ctttgcagat tttccgagct
      421 tgcgatccgc ttgtagaacg acagacgcat attcacgtca ggcagataat cgtccggcaa
      481 taaggccggt acacgcaatt caatctccgc ttggtgttgg gtgatttcat cgaggctcgg
      541 ttctcgccct tcttgcaaag ctttgaccgc attttcgagc aagtccatat agagcgaaaa
      601 accgatgctt tcaatctgtc cgctctgctc cgaaccgagc aattcgcctg caccacgaat
      661 ttctaaatcg tgggtcgcca acgcaaaacc ggccccgaga ttatcaatgg tactcatcgc
      721 ctccaagcgt tgctcggcat ctttggtgag cgttttcggg tgcggtgtga gcatataggc
      781 gtaggcttgg tggtgcgagc gtccgacccg tccacgcagt tggtgcagtt gtgccagccc
      841 gaatttgtcc gcccgttcga taataatggt attggcagtc ggcacgtcaa tccccgtttc
      901 aataatggtg gagcaaacca gcaggttaaa gcgttgatga tagaaatcgc tcatcacccg
      961 ctccaattca cgctcacgca tctgcccgtg tccgatcaca attcttgcct ctggcacgag
     1021 ttcagccaat ttctcggcac aattttcaat ggttgccaca tcgttatgga ggtagtaaac
     1081 ctgcccacca cgcagaattt cacgcaaaat tgcctcacgg attaccattt catcactttg
     1141 tcgcacaaag gttttgatta aaagtcggcg agccggcggg ctggcgataa tcgagagatc
     1201 acgcattccg ttgagtgcca tattcagcgt tcttggaatt ggcgtggcgg tgagggtcag
     1261 aatatccaca ttggcacgca gttgtttgat tttctctttt tggcgtacgc cgaaacggtg
     1321 ttcttcatca atcactaaaa gcccaagatc acggaacgct acatcgtctt gcaacagttt
     1381 gtgcgtgccg accaaaatat ccactttgcc ctccgccaca ttttgcaaaa tcgttttctg
     1441 ctctttggcg gttttaaagc gagacagcac ttccacattc acagggtaat tggcgaaacg
     1501 gtctttgaaa ttctcataat gctgttgggc gagcaaggta gttggggcaa gaataaccac
     1561 ctgtttatgg ttcatcactg ccaagaaagt cgctcgaatg gccacttcgg ttttgccgaa
     1621 gccgacatcg ccacacacca atctatccat cgctttggct tggcacatat cgctaattac
     1681 ggcattgatg gcaattttct gatcgtccgt ttcctcataa gggaaagtgg cactaaactg
     1741 atcaaattcc gctttatcgt aggcaaacgc aaaaccggtt tggctttctc gtttggcata
     1801 aacatcaagc aattctgccg ccacatcacg gattttttcc gctgcttttt ggcgggtttt
     1861 cgcccacgct tcactgccta atttatgtag cggcgcagtt tcatccgccc caccgatata
     1921 acggctgatt aaatggagcg aggcaaccgg cacatagagt ttggcatcgt tagcataatg
     1981 tagcacgaga tattcggctt tgatcccacc agcgtccagc acggttaagc caccgtaacg
     2041 ccccacgccg ttttccaaat gcaccaccgc ctgtccgatt ttgagttctg ctaaattacg
     2101 gattaaggta tcaggattga cggctttgcg gtttttctca cgccgtgttt gcacacgttc
     2161 gcccagcagg tcggtttcgc agatgatggc aaggttgcgg gcgggcacag gacccgcccc
     2221 tacgtagtta ttatcatttt cgccatttgt atttaattca tcatcgtagg gacgggtccc
     2281 gtgcccgccc gtttccacca caaacccttg atcaagcggt gaaataatca gggaaaatgg
     2341 caaaccttgt gcttcatcta atgaattgac ctgcttcggc ttcaccccaa gcggagcaag
     2401 caaatccagc aaggtttctc ttcgcccttc gctctccacc gaaaacagaa tgttgccgtc
     2461 aaatttttgg cggaattgct ggaaagcaga aaacggctct ttggcagagg attgaatagc
     2521 aatatccggc aagttagcca catttgcatt cattttcgct gctgatttac ggattttctc
     2581 cgaagttatc gttaaacggg gatagccttt cagccagcga tttacctcat caatcgcaaa
     2641 ccagagatca ctcggcggca gtagcggtcg catcggatcc actcggtggc tttcatagcg
     2701 gttttgggta tcttggtgaa attgttcagc tttttgctga atatcggcga aagtgataaa
     2761 cagcgtttgg gcagggaaat agtcaaacag acttgccatt tcctcaaaaa acaacggctg
     2821 ccaatactca atccctgcat ttaaaatgcc tttgctgact tgctgataaa tatgctccgg
     2881 ctcacggcgg atttcaccga atttttcacg gaatttggct ctaaaatgct cgatcccgtt
     2941 gctatcagtc gggaactcgt gtgccggcag taaattaatt tcggcaattt ccgttttggt
     3001 gcgttggtta tccacatcaa aagtgcggat actgtcgatc tcatcatcga aaaaatcgag
     3061 gcgaaacggc tcttctgctc ccatcgggaa taggtctaaa atcgagccac gcacggcgta
     3121 ttcaccatat tccaacacct gttccaccgc ccgatagccg gcattttcca gttgtaaccg
     3181 cagtttttga atcgaaaaac ggtcgccttt tttaatcaac agcacattgt tagcgagata
     3241 attcggcggg cagaccttct gcattaaggt gttgatcggc aacagcaaaa tctgctgtcg
     3301 cccttgttgc agttcaaaca gggcagaaag tcgggcggag ataatgtctt ggtgcggcga
     3361 aaaattgtca taaggcaagg tttcccaatc ggggaacagt tgcactggca gccctgcaaa
     3421 ctgaggcaag gctttttcca agcgtaacgc cgtgcgggtg tcgggtgtga caaccaccgt
     3481 caagccgtaa aaggctcggg cagcttggct gatcgcaagg gtatcggaat gcccgacaag
     3541 gttaccgagg gtttgatgat cttgaaaatt gccgtcagat ttcggcagat taagtgaaat
     3601 taaagacata caagcggtct gttttaaata aaaaattgca aattgttgct tagtttacta
     3661 aagcacggct gattgtgcaa aataagcgga gaaattatgt tatctagatc gcattgcgaa
     3721 aaaattctga taaaatgttc ccctttttta actcttactc acacatatta tgaaaaataa
     3781 aattttagga agtgcattaa tgattgcagg cactaccatt ggtgcaggaa tgcttgcaat
     3841 gccactcacc tctgcaggaa tgggttttgg ggcaaccgca ttactcttag tcggattatg
     3901 ggctttattg gcctacaccg gtttattatt tatggaagtt tatcaaaccg ctccgcaaaa
     3961 agatgttgga gtggcaagcc ttgccgagca atactttggc ttaatcgggc gtgtattagc
     4021 cactttcagt ttgctcgtac tactttatgc cctacttgcg gcttatatta ccggtggtgg
     4081 ttcactgtta gccggtgtaa tgcctgaaat ggcagatgct gatatgaaac tgaaaatcag
     4141 cattttaatc ttcactgcgg tattaggtac atttgtggta gtcggcgtga aatcggttga
     4201 tggtttaacc cgagtactgt ttttgggaaa aattgttgct tttattgccg tgttgctgat
     4261 gatgctgcct aaagcgaagc tggaaaactt gacagcaatt ccattagata acttactcat
     4321 tatttctgct atacccattt tcttcactgc gttcggtttc cacgttatta tgggaacaat
     4381 taatagctat ttagacgcag atatcggcaa aattcgtaaa tctatttata tcgggacggc
     4441 aattccgcta gtggcttatt tattgtggca gctcgctaca cacggggtat taagccaaaa
     4501 tgaattcacc acaattttga cacaagatcc gaccttaaac ggcttggtga aagccactag
     4561 ccagattacc ggtagtacga ttttaggcga aatggttcgc ttattctcag tgcttgcatt
     4621 gattacctca tttttagggg tttcgatggg aatttttgaa ggcgtgggcg atttgttaaa
     4681 acgcttaaat ctaccgacta accgtttatg gctgaccatc gccaccttta caccgccaat
     4741 tctgttcgct ttattctatc cgaacggctt tattaaggca ttaggttacg ccggtttact
     4801 ctttgcgttc tatggtatga ttttacctat cggcttagcg tggaaagcac gtcgccaata
     4861 tccgaattta ccttaccgtg tgattggtgg taatttggca ttagtggttg cgttaattgc
     4921 cggtattatc attatggtca ttccgttttt aattcaggct ggctatttac cgcaagcggt
     4981 aggctaatat tgaaaagggc tacgattcaa tatcgtagcc ctttctataa gttacaagcg
     5041 gtcatttttc gatgactttt tacagattag ccaatcaact gatacagcgc cttaatttga
     5101 tgctctttta atgccccgcc aattttctct ctgccgacaa aaatcttaaa cattgcgttg
     5161 ccttttttat taatgaaaat cagtgaaaca gtacgttttc ccataaaagg acgatccaac
     5221 aaataaattt tggcacaatt ttcgtagcgt agatgccctt gtaagcctcc gccctcttta
     5281 tgttcgaaat tgtaaaatcc acgagaaatt gaaccgcttg ggagctttcc cacaaactca
     5341 ataatggtat cttccgtatg cgaaatcagc gtaatcgcat cgtcccattc gtggatttgc
     5401 tgtagcactt cggcaaaacg ttccccttcg gttactttta gcatttcagc cggtaaattc
     5461 agcattacct cttccagcgt gcattgatat tttgccgcta acatttctaa aatttgcccc
     5521 ggattttcgg ctaattcatt gcgtaaggcg gtttgttgtt cgattgatat cattaaatgt
     5581 tcctttaagt aaagagagtt tcttagctct ctaagtaaat ttgtaaatta gaaacggtat
     5641 tcaaaattag ccgagaagtt acgtccaggt tcataatagc gttgtaatcc aggatagcca
     5701 ccgtaagttg cattttcacg tactccaaca gaattaacga ttccacctgt tgtatttaaa
     5761 ccacgtaaag tatcccaagt atggtattta cggttaaaca aattataaat accggcacgt
     5821 aatgtaatat tctcagttgg tttatagaaa ccgtataaat ccacaacaaa ggctttagca
     5881 tttaagtgct tccaggtatc taaacctgtc tcgtgagaat attcagtaca acgaatgtca
     5941 tattcatccc cccaataagg gtaatacgga tttggcgttc tatcctcttt aaggcatttt
     6001 tccggtaatg tttttaaata ttttgcatct ttcgcctttt tagcccccaa gaatgtgaag
     6061 cgagaaaata ctccccattt accttcaggt tgttcataat ctaagccaac tacggcttta
     6121 atcggctgaa tagaaagtaa atctgccccg ttagacatac tacctttgct gtaaccgaag
     6181 gctccaaata gcttccagcc ttcaggtatt ggcatagctg aatttaaatt ccatttacca
     6241 ctcacttcta gcccgtagat tttcgcagaa tcaatatttt gcatttgctg taccgggcgc
     6301 caaaactctc taccatatgc ttggtaaaca tcccaggttt cttgctccca taaaaaatct
     6361 ttatatttag tatggtggaa attaactgag agcagcccaa cattgccact accttgcaaa
     6421 cttaaatttt ggcttaaact acgttccgct tttaggtttg gattggacat ccaagctcct
     6481 gcagcattat cacggtaatc aaaatacatt tctgatgcat ttggaatacg gaagcctgtc
     6541 ccaatgttat atcctacttt ccatgcctca gtcagatgtt tttctaaacc ggtaacccaa
     6601 gttagttgat taaatttcgc attgtcagtt tttacgcagt tacgacaaac aagaccattg
     6661 agttcttgtt gttcatactt cgcccaatca taacgaatgc ctagcgttgc aatccaatca
     6721 tgcgttaaat caatcttatc gtgcaatgaa aaagtgtgct gtttcgtttt aattgggtac
     6781 atcatcgtag aatcgggaga atttacccaa acaccggata aataaaccga gtccttatgt
     6841 aatacatcga aatctctctc tgcagtagag gctttgaaag atagctgatg agtccattta
     6901 ccaaattcta atgcttggct gtctaaacgg aaatttaagc gtttcaattc agtattaaaa
     6961 atacgaatat tattgtcact tggttttctt tgtttagcag ggctccagtc tgttgcatct
     7021 tcagcgcgtg taccttcata gttataagca gatgtttcag tatcttgata atctgcatct
     7081 atcttcgcta gagatatcca ttttgactct gggaaatact cataaaaaac atttactgta
     7141 tcacgtttac tacggtcttc cgcttcacgc caaa
//
LOCUS       DANLXF010000122         7168 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.122, whole genome shotgun sequence.
ACCESSION   DANLXF010000122 DANLXF010000000
VERSION     DANLXF010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7168
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.122"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            69..1160
                     /gene="ulaG"
                     /locus_tag="PXD66_001634"
     CDS             69..1160
                     /gene="ulaG"
                     /locus_tag="PXD66_001634"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDL6319919.1"
                     /translation="MSKIQEITRESWILSTFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     CVGVWIKTPAGANICMDLWCGRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRAQPMVL
                     DPFAINEVDFILASHYHSDHIDVNVAAAICNNPKLDHVKFVGPWHCAELWKKWGVPEE
                     RIIVVKPGDVVKLKDVEIHALDSFDRTCLVTLPVEGAEERGGELAGLCPSDEEMGRKA
                     VNYVFKTPGGTIYHGADSHYSIQFAKHGKQFDIDVALNNYGENPVGIADKMTSVDLLR
                     MAECLRCKVIIPVHHDIWTNFMASTQEIIDLWRMRKDRLQYQFHPFIWEVGGKYVYPR
                     DKDLIEYHHPRGFDDCFEQEPNIQFKSML"
     gene            1244..1990
                     /gene="ulaR"
                     /locus_tag="PXD66_001635"
     CDS             1244..1990
                     /gene="ulaR"
                     /locus_tag="PXD66_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HDL6319920.1"
                     /translation="MNESYRHKELLALLEERKALSTADIMQLLNISPATARRDISKLD
                     GQGKLKKVRNGAEAILAPTFLQHTKITNNAQEKRRIAEAAGRLCKDGESLILTCGSTM
                     EMLGEVLCGRKLQIITNYLPLANQLIAGCHDDVVIMGGQYNKHKKVMLSINTQNEMTY
                     AATTMFTSGKGLTSEGLFKTDMIIANAEQQMAKHAERYVVLLDSTKLGKKVGMLFKEF
                     DKIDLLITGKEADPTIIQALRNKGLDIILA"
     gene            2045..2905
                     /locus_tag="PXD66_001636"
     CDS             2045..2905
                     /locus_tag="PXD66_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HDL6319921.1"
                     /translation="MRKHKLGIYEKALPKNISWQDRLSIAKVCGFDFVEMSIDETDER
                     LARLDWSKKERAKLVKTIINTGVTIPSMCLSGHRRFPFGSCDEATRQKAYEIMEKAIQ
                     LAVDLGIRTIQLAGYDVYYEEQDEGTIQRFQQGLEWAVELAASNQVTIAVEIMDTQFM
                     SSISRWKKWDEIIKSPWFTVYPDLGNLSAWNDNVAEELKLGIDKISAIHLKDTYKVTE
                     TCKGQFRDVPFGDGCVDFAACFKTLAELNYRGAFLIEMWTEKAEEPITEIINARRWIE
                     QKMQEGGFTL"
     gene            2902..3621
                     /locus_tag="PXD66_001637"
     CDS             2902..3621
                     /locus_tag="PXD66_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDL6319922.1"
                     /translation="MTQLTELTPALQALRERVLKANLELPKYGLVTFTWGNVSEIDRE
                     TGLVAIKPSGVEYDAMTVEDIVLVDLQGNRVYGDKKPSSDTETHLELYRQFTDIGGVV
                     HTHSRNAVGWAQAGRDLLALGTTQGDYFYGAIPCTRKMTAEEIAGSYELETGKVIVET
                     FRTRGIEPKAVPGVLVHSHGPFTWGKDGHDAVHNSVVLEEVAYMNFISHQIRPQIGPM
                     QQELLDKHYLRKHGANAYYGQ"
     gene            3842..6319
                     /locus_tag="PXD66_001638"
     CDS             3842..6319
                     /locus_tag="PXD66_001638"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012311.1,HMM:TIGR02601.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDL6319923.1"
                     /translation="MYLLEGNQKVKNRNYFVAIATILALSPEVNATELSDQLASRTRY
                     YPSATDSYYSYYKYAPIYSGYYTSNSRNGYSSWYYPGSNAGVTTRTVSTTVNGITVTK
                     VYTTHNGRTTVRTTYAPAVNKPIVNKPTPIANKPVVDTPTQPVIDANPIMDPPVQPIV
                     DNKPITDTQPNPITDNKPVIESPIDDTRPVIIRPTTPVNLHPEPVAVGVPNVGIRWND
                     RSRGYNIDDPNNKQNVKLTGEGVKVGILDSGFKNYLMQDDILKKFGNRAVIIETGRTA
                     PASATHGIEVAEMVGGGNSRNGVASKSTLLLADITKTTNAGTGLSATAPIYYDLWNRG
                     ARIFNQSYGIPKPLTYFNNNRNSSYYYLHQFDGNVLQFYKDKVKEGGLFVWAAGNNRG
                     DLDPSLQAGLPHYEADLEKGWISVVAVATRTNQGLGNFEWSNLLPYSQAGVAKNWTVT
                     AMGDYIFNLGGRNIIASGSSFAAPAVTGTAALVKQKYPWMDGNLIKQSILSTATDIGA
                     PGVDDVYGWGLLNIEKATNGPARFGKRLALADNVTVNIPNGSYEFSNNINGDAGLVKN
                     GQGELILSGASTFEGDTTLNEGAVTINGKKYVSKVNVSPSGTLVVNNTTLENGVNNEG
                     TVVNQGYSTIKRAYVASAQSTLVSDLGSNLNVKGEVDLNGSTLSLAAVKEGKAQYVTQ
                     KGLSMSAITSDSAIKGNFAAIETSGLLTATAEKTNENEVKATVSRKNVATALESSFSA
                     LDNQIEGNNGSTNATNDEFAQQAATLQNSIANINTQSVVLDSLSGQIYASAQALTFEN
                     SETVNKDLSNRLVMLGTLDNVGETAGV"
     gene            6329..7144
                     /locus_tag="PXD66_001639"
     CDS             6329..7144
                     /locus_tag="PXD66_001639"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDL6319924.1"
                     /translation="MYGNGTLKEIGFGEGKTNTYAGQIGFDKPVTDHVILGAALNYSK
                     AKVNFDRYGGSSKADGIGASLYARIGNKHKTPWYLQGRAGYGSVDSDVERHIILADNN
                     ASTAKINHRDRVFSAYVESGYDFKQGRFALTPFVGFSHDVVRRGAFSEQNSQFGLTAD
                     KATYKQTSGLIGLRTSLEVNLAGMKTTFQGYVNHQKAFNREDLSFKASYTGLQDAKFD
                     VKGIGLAKHKTWVGIGALAEVNKNTSLYVNYDLKLAKNSGHNNVVTAGIRINF"
BASE COUNT         2235 a         1337 c         1622 g         1974 t
ORIGIN      
        1 tttttgtgat tgtttatgat tggttttgat tgcattgttc ttattttagc gtaaatcagg
       61 agactattat gagcaaaatt caagaaatca ctcgtgaaag ctggatcctt tcaactttcc
      121 ctgaatgggg tacttggttg aatgaagaaa ttgaagaaga agttgtaccg gaaggcaatt
      181 ttgcaatgtg gtggctaggc tgtgtcggtg tgtggattaa aaccccagct ggtgcgaata
      241 tctgtatgga tttatggtgc ggtcgtggta aatccaccaa gaaagtaaaa gatatggtgc
      301 gtggtcatca aatggcaaat atggcagggg tgcgtaagct ccagcctaat ttgcgggctc
      361 aaccgatggt gttagatccg tttgcgatta acgaagtgga cttcattctc gcttctcact
      421 atcactcaga ccatattgat gtaaacgtgg cagcggctat ttgcaataac ccgaaattag
      481 accacgttaa atttgtcggc ccttggcact gtgcagaatt atggaaaaaa tggggcgtgc
      541 cggaagaacg tattattgtg gtaaaaccgg gcgatgtcgt gaaattaaaa gatgtagaaa
      601 ttcacgccct agattcattt gaccgtacct gtttagtgac cttaccggta gaaggtgcag
      661 aagaacgtgg cggagaactt gcaggcttat gcccatcaga tgaagaaatg ggacgcaaag
      721 cggtgaacta tgtgttcaaa accccgggcg gtacgattta tcacggtgca gattctcact
      781 actcaatcca attcgctaaa cacggtaagc aatttgatat tgacgtagcg ctgaataact
      841 atggcgaaaa cccggttggt attgcggata aaatgacttc agttgattta ttacgtatgg
      901 cggagtgttt acgctgtaaa gtgattattc cggttcacca tgatatttgg acaaacttta
      961 tggcaagtac gcaagaaatt attgatttat ggcgtatgcg taaagatcgc ttacaatacc
     1021 aattccaccc atttatttgg gaagtgggcg gcaaatatgt ttacccacgt gataaagact
     1081 taattgaata tcatcaccct cgcggctttg atgactgctt cgagcaagag ccaaatattc
     1141 aatttaaatc catgctatag gtgagcagtc gccactctca ctggagagtg gcgttttagc
     1201 aagttcatta tggagcagta gtgattagta atgggataaa gtagtgaacg aaagttatag
     1261 acacaaagag ttattagccc tgttggaaga gcggaaagca ctgtcaacag cagatattat
     1321 gcagctgtta aatatttctc cggcaacagc aagacgtgat atcagtaaat tagatggtca
     1381 aggtaaatta aaaaaggtta gaaacggagc agaagcaatt ctggctccta catttttaca
     1441 gcacacaaaa ataactaaca atgcgcagga gaaacgtaga atagcggaag ctgccggtcg
     1501 tttatgtaaa gatggggaaa gtcttattct gacgtgtggt tccacaatgg aaatgctcgg
     1561 tgaagtatta tgtggtagaa aattgcaaat tattaccaat tatctaccgc ttgcaaacca
     1621 gttgattgca ggttgccacg atgatgtagt gattatgggt gggcaatata ataaacataa
     1681 aaaagtgatg ctctcaatta acacccaaaa tgagatgact tatgccgcta ccacaatgtt
     1741 taccagtggc aaaggcttaa ccagcgaagg attatttaaa accgatatga ttattgccaa
     1801 tgccgagcaa caaatggcaa agcacgcaga acgttatgtg gtgttattgg atagcaccaa
     1861 gctcggcaaa aaagtcggta tgctgtttaa agagttcgat aaaattgatt tattgattac
     1921 cggcaaagag gcggatccga ctattatcca agcattaaga aacaaaggcc tggatattat
     1981 cttggcttag cctgtagcgg tcaaatttaa tcaaaatttt acttatcatc ttcaagggaa
     2041 aactatgaga aaacataaac tgggtattta cgaaaaggcc ttgccgaaaa acatttcgtg
     2101 gcaagaccgc ctttctatcg ccaaagtctg cggttttgac tttgtggaaa tgtcgattga
     2161 cgaaacggac gaacgcttag cccgccttga ttggagcaaa aaagaacgtg ccaagctggt
     2221 gaaaaccatc atcaacaccg gcgtgaccat tccgtcaatg tgcttgtccg gtcatcgccg
     2281 tttcccgttt ggttcttgtg atgaagcgac ccgccaaaaa gcctatgaaa ttatggaaaa
     2341 agccattcaa ctggcggtcg atctcggtat tcgcaccatt cagcttgccg gctatgacgt
     2401 ctattacgaa gagcaggacg aaggcacaat ccaacgcttc caacaaggct tagaatgggc
     2461 ggtggagctg gcggcaagca accaagttac cattgcggtg gaaattatgg acacccaatt
     2521 tatgagttcg atttcccgct ggaaaaaatg ggacgagatc atcaaatcac cgtggttcac
     2581 cgtgtatcca gatttaggca acctctcagc gtggaacgac aacgtagcgg aagagctgaa
     2641 actcggcatc gacaaaatct ctgccattca cttaaaagac acttacaaag tgactgaaac
     2701 ctgcaaaggg cagttccgtg atgtgccatt tggtgacggt tgcgtagatt tcgccgcttg
     2761 ttttaaaacc cttgccgagc tgaattaccg tggcgcattc ttaattgaaa tgtggacgga
     2821 aaaagccgaa gagccgatta ctgaaatcat caacgcccgc cgttggatcg aacaaaaaat
     2881 gcaagaagga ggatttaccc tatgacgcaa ttaactgaat taacccctgc attacaagcc
     2941 ttaagagagc gtgtgttaaa agccaattta gaattaccga agtatggctt ggtaactttc
     3001 acttggggta acgtaagtga aatcgaccgt gagaccggtt tagtcgccat caaaccttcc
     3061 ggcgtagaat acgatgcgat gacggtggaa gacattgtat tggttgattt acaaggtaat
     3121 cgagtttacg gagataaaaa accgtcttct gatacagaaa cgcacttaga actttatcgc
     3181 caattcactg atattggcgg tgtggtacac actcactctc gcaatgctgt aggttgggca
     3241 caagcagggc gtgatttatt ggcacttggc accactcaag gcgattattt ctacggtgcg
     3301 attccttgta ctcgcaaaat gaccgcagag gaaattgccg gcagttatga gctggaaacc
     3361 ggaaaggtga ttgtcgaaac tttccgaact cgaggtattg aaccgaaagc agttccaggt
     3421 gtgttggtac attcacacgg tccatttacc tggggaaaag atggacacga tgctgtgcat
     3481 aattctgtgg tattagaaga ggtggcttac atgaatttta ttagccatca aattcgccca
     3541 caaattggcc caatgcaaca ggaattatta gataagcatt atctgcgtaa gcatggtgca
     3601 aatgcttatt atgggcaata gctaataacc taaaaattat tttccaaatt atcaacagct
     3661 ttcgaggggc tttctataat cggaatagct cctcagtctg tttgagttta tggatagatc
     3721 cacaaataat taccattttc cttttcttga ttttaatttt gatttttgtt ctaatctctg
     3781 cctcttgttt ttacaagtct attaaggatc cgtagtagat ttgaaataaa caagagtatc
     3841 tttgtattta ttagaaggaa accaaaaggt gaaaaataga aattattttg ttgcaatagc
     3901 aacaatatta gccttatcgc cagaggttaa cgcaactgaa ctttcagatc agcttgcaag
     3961 tagaacaaga tattatcctt ccgccacgga tagttattat agttactata aatatgcacc
     4021 gatttattcg ggctactata catcgaatag ccgtaatggt tattcgtcgt ggtattatcc
     4081 aggtagcaat gcaggtgtaa caaccagaac tgtttcgaca acagttaatg gcattacagt
     4141 tacaaaagtt tatacaacgc ataatgggag aacaaccgtt cgtaccactt atgctcctgc
     4201 tgtgaacaaa ccaattgtaa ataaaccgac accgattgcc aataaacccg ttgtagatac
     4261 gccaactcaa ccggttattg atgccaaccc aattatggat ccgccagtcc aaccaatagt
     4321 tgataataaa cccatcactg atacacagcc taatccaatt acagataaca aacctgtcat
     4381 tgaatcgcca attgatgaca cccgtccagt tatcattagg cctacaacac cggtaaatct
     4441 tcatcctgaa cctgtagctg ttggtgtacc caatgtaggt attcgatgga atgaccgctc
     4501 tcgtggttac aacattgatg atcctaataa caaacaaaat gttaagttaa ctggtgaagg
     4561 tgttaaagtc ggtatcttag atagtggttt taaaaactat ttaatgcagg acgatatact
     4621 caaaaaattt ggtaatcgcg ctgttattat tgaaacaggg cgtactgccc cggcttctgc
     4681 aacacatggt attgaggtcg cagaaatggt tgggggaggg aactctcgca atggagtagc
     4741 gtctaagtca acgttattac ttgcggatat taccaaaaca acaaatgcgg gtacaggatt
     4801 atccgcaaca gctccaattt attatgactt atggaatagg ggagcccgta tttttaacca
     4861 atcatacggt attccaaagc ctcttactta ttttaataac aatagaaatt ctagctatta
     4921 ctatttacac caatttgatg gtaatgtgtt gcaattctat aaggataaag taaaagaggg
     4981 gggcttattt gtctgggctg cggggaataa ccgtggtgat cttgatccat cactccaagc
     5041 tgggttacct cattatgaag ctgacttgga aaaaggttgg atttctgttg ttgcggtagc
     5101 aaccagaact aatcagggat tgggtaactt tgaatggagc aatttattgc catattctca
     5161 agccggtgtg gcaaaaaatt ggaccgttac cgcaatgggt gactacatct ttaatttagg
     5221 aggtagaaac attattgcta gtggttcatc ctttgcagct ccagccgtta cgggtacagc
     5281 agctctcgtg aaacaaaaat atccttggat ggatggtaac cttatcaaac aatctattct
     5341 ctcgacggca accgatatcg gcgctcctgg agttgatgat gtatatggat ggggtttact
     5401 taatattgaa aaagccacta atggccctgc gcgttttggt aaacgactcg ctttagccga
     5461 taacgtgact gtaaatattc caaatggaag ctatgagttt tccaataata ttaacggtga
     5521 tgcaggcttg gtgaaaaatg gccaaggcga attaatttta tcgggtgcta gtacttttga
     5581 aggggataca acactcaatg aaggtgctgt cactattaac ggtaaaaaat atgtgtctaa
     5641 agtaaacgta tctccaagtg gtacattggt tgtcaataat accacgttag aaaatggcgt
     5701 aaataacgag ggtacagttg tcaaccaggg ttattctacg attaagagag cttacgttgc
     5761 ttcagcacaa agtacgctag tgtcagattt aggttcaaac ttaaatgtaa aaggagaagt
     5821 tgacttaaac ggctctactt tatcactagc tgcggtaaaa gagggtaaag ctcagtatgt
     5881 gacccaaaaa ggtttatcaa tgagtgctat tacttctgat tccgcaatta agggtaactt
     5941 tgctgcaata gaaacctcag ggttattaac tgcgacggcg gaaaaaacca atgagaatga
     6001 agtaaaagca acagttagcc ggaaaaatgt cgcgactgct ttagagagct cattttcggc
     6061 attggataac caaattgaag gaaataatgg ttctacaaat gctacaaatg atgaattcgc
     6121 acaacaagcg gctactttac aaaacagtat tgcaaacatc aatacgcaaa gtgtggtttt
     6181 agatagctta tctggtcaaa tttatgcgtc tgcgcaagcg cttacttttg aaaactcaga
     6241 aacagtgaat aaagatctct ctaaccgtct tgtaatgtta ggaacgctgg ataatgtagg
     6301 tgagactgct ggtgtgtgag taacgggtat ttatgggaat ggtactctaa aagaaatagg
     6361 gtttggtgaa ggtaaaacca atacctatgc tggccaaata ggttttgata agccagttac
     6421 agaccatgtg attttaggtg cggcacttaa ttattctaaa gcaaaagtga acttcgatcg
     6481 ttatggcggt agttcaaaag cagatggaat aggtgcatca ttatatgccc gtataggcaa
     6541 taagcataaa acaccttggt atttacaagg tcgagccggt tatggcagtg ttgattcaga
     6601 tgtagagcgt catattattt tggcggataa taacgcaagt acagcaaaaa tcaaccaccg
     6661 agacagagta ttttctgctt atgttgaatc aggctatgac ttcaagcaag gtcgctttgc
     6721 gttaactcca tttgtcggtt ttagtcatga tgttgtcaga cgtggcgcat tcagtgagca
     6781 aaacagtcaa tttggtctca ccgcggacaa agcaacctat aaacaaacaa gcggattgat
     6841 tggtttacga acatctttag aggtgaattt agctgggatg aaaacgactt tccaaggcta
     6901 tgtaaatcat caaaaagcgt ttaatcgaga agatcttagc tttaaagcaa gttatacagg
     6961 tttacaagat gcaaaattcg acgtaaaagg tattgggctt gcaaaacata aaacgtgggt
     7021 agggattggt gcattggcgg aagtaaataa aaatacttcc ttatatgtga attacgacct
     7081 taaactcgca aaaaatagtg gtcacaacaa cgttgtgact gctgggatta gaattaattt
     7141 ctaactgaat ctttttaaac aaaacggg
//
LOCUS       DANLXF010000123         7107 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.123, whole genome shotgun sequence.
ACCESSION   DANLXF010000123 DANLXF010000000
VERSION     DANLXF010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7107
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.123"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..749)
                     /locus_tag="PXD66_001640"
     CDS             complement(<1..749)
                     /locus_tag="PXD66_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005567524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL6319925.1"
                     /translation="MITVKNLHHKIGNATILNNINLTIPNGGITALIGANGAGKSTLL
                     SLIARLQPIQQGEIHLNELNIANTASQTIARQLAILTQDNVIHSRITVKDLLLFGRYP
                     HHQGRITSHDNAIVEEALVRFDLSTFSHRFLNELSGGQRQRALIAMTFCQQTDHILLD
                     EPLNNLDMFHARELMKLLRKLTDELALTTIMVVHDINIAAAYADTIIAMKDGGIKMIG
                     TPKEIITTENLKTVFNLDAEVLECQGKPLVI"
     gene            complement(769..1645)
                     /locus_tag="PXD66_001641"
                     /pseudo
     CDS             complement(769..1645)
                     /locus_tag="PXD66_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
     gene            complement(1698..2666)
                     /locus_tag="PXD66_001642"
     CDS             complement(1698..2666)
                     /locus_tag="PXD66_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL6319926.1"
                     /translation="MFSFFRLNLFLLILLIPISASVGVADFHWSEIFHSSEQSQIFFI
                     SRLPRTLAVILVGATLAVAGMVLQIVLKNRFIEPSMIGTSQSAAIGILLASLLFPTSA
                     LLLKMSIATLSALMGMGLFMLLIRQLPPHQQLMLPLIGIVFGNVIEAISTFIAYETDS
                     LQVLSIWFSGDFSGILAGRYELLWITAILAVVVYVMADQLTIAGLGENISTNLGINYK
                     QMTWLALIVVAMITAIVVVTVGQIPFIGLVVPNIVSRIAGDRLRKNLPAVVVLGANLV
                     LICDILGRAINAPYEVPISTIFGIIGTVIFLYLLLKEKRAEEKARG"
     gene            complement(2760..3659)
                     /locus_tag="PXD66_001643"
     CDS             complement(2760..3659)
                     /locus_tag="PXD66_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005700892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL6319927.1"
                     /translation="MIKKALFALTATAVFAASAFAKDVTIPTARGEVTFKETPSKIAV
                     FDTGSLDTLQALGVKIDGMPEFKVLSYLKPSADKAVNVGTVFEPDLEALNSLNPDLII
                     VGTRTAKKFDEVSQIAKTIDMTDNGDKLIESGLQRLDSFGKLFNKEAETEKLKAEINT
                     LLDQTKAAVKGKGNGLIILVNGGKISAFGSGYRLSWIHDNLGIPMADSSIKNTGHGQP
                     VSFEFIQKLNPDWLFVLDRIAAIGQEGQSAQQVLDNELVRQTKAWKNGNIVYLSAASY
                     LAPGGYEQMKTDLTNIKTAFEKK"
     gene            3833..5296
                     /locus_tag="PXD66_001644"
     CDS             3833..5296
                     /locus_tag="PXD66_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDL6319928.1"
                     /translation="MAEYSGTTQEKMKDKQVEQINISPHSIEAEQAVLGGIMLSNDHW
                     DNVAERVQPHDFYNYAHRIIFEQMAELVRHNKPIDIITLDQALKDKGVLQDVGGFAYL
                     AELSKNTPSAANILAYADIVRDRSDLRSVISAGNQIAEMGYKTKGRNSKEVLDEAERI
                     VFEIAEKRNNANEGPQRIDDILLKTLARMDTLSKNRHNGGVTGVSTGFVDLNKKTAGL
                     QPSDLIIVAARPSMGKTTFAMNLCENASLVDIEDKDDLDEQGNPKKKPAKPVLIFSLE
                     MPADQIMMRMLASLSRVDQTKIRTGQISDDEDTAKISSTMAILQERNNIYIDDSSGLT
                     PTELRSRARRIYRENGGLSLIMVDYLQLMRAPGFADNRTLEIAEISRSLKALAKELEV
                     PVVALSQLNRSLEQRADKRPVNSDLRESGSIEQDADLIMFIYRDEVYNDNSDLKGIAE
                     IIIGKQRNGPIGRVRLTFQGQYSRFDNYTGGHYDDEY"
     gene            5286..5591
                     /locus_tag="PXD66_001645"
     CDS             5286..5591
                     /locus_tag="PXD66_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HDL6319929.1"
                     /translation="MNTKPTQPITLCAIYRSKAKEGMYLYVPKRDQFEQVPETLRQLF
                     GKPEFVMMFNLTGEKPLVRAKNEEVLQLLIEQGYYLQMPPPPENLHKAFIAQQKGEK"
     gene            5591..6040
                     /gene="nrdR"
                     /locus_tag="PXD66_001646"
     CDS             5591..6040
                     /gene="nrdR"
                     /locus_tag="PXD66_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HDL6319930.1"
                     /translation="MRCPFCSTEETKVIDSRLAASGYQIRRRRECISCKERFTTFESA
                     ELVVPYVIKNNGSREPFDAEKLRTGLRRALNKRPVSADDVEKAISQIIIQLQAMGERE
                     VPSRHIGTLAMNALKQLDKVAYIRFASIYLSFSDIEEFIKEIEKLKE"
     gene            complement(6114..6296)
                     /locus_tag="PXD66_001647"
     CDS             complement(6114..6296)
                     /locus_tag="PXD66_001647"
                     /inference="COORDINATES: protein motif:HMM:NF015431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL6319931.1"
                     /translation="MALLADSTVDRLVAENGLVEVLGETENPKFDLTMIYAAHRYPSS
                     IVRAFVGFVLEQVKAN"
     gene            complement(6265..6975)
                     /locus_tag="PXD66_001648"
     CDS             complement(6265..6975)
                     /locus_tag="PXD66_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL6319932.1"
                     /translation="MNLNTLRLFVAVIQHGSLSKASERLNVPIATISRQIADLEKELN
                     IQLFDRQKSGVKPTMAGQRLYEQVHLSIEQLATAKEVLFEEQGNLKGKLRLSASPACE
                     PVLAWIADFQAAYPQVQVHCTLTDRLLDLSADGIDVAFRIGQLHGEQFIAKKVGQIGS
                     KWVAHPKFLARFGTPSTVKDLKNFPIAAWAKNEASVITLGTGRQAVEIPYLFSSNDSY
                     AIEYMAKSSGSACRQHGG"
BASE COUNT         2244 a         1428 c         1461 g         1974 t
ORIGIN      
        1 tgaataacta aaggtttgcc ttggcattct aacacttcgg catcaagatt aaacactgtt
       61 tttaaatttt cagtggtgat aatctctttt ggggtgccga tcatcttaat tccaccatct
      121 ttcattgcaa taatagtatc tgcgtaagca gcagcaatgt tgatgtcgtg taccaccatt
      181 atagtagtta aggcgagttc atcggttaat tttcgtaata atttcattaa ttcccgagca
      241 tggaacatat ctaaattatt cagtggttca tctaataaga tatgatcggt ttgctgacag
      301 aaggtcattg caattaatgc ccgctggcgt tgccctccgg ataattcatt taagaatcgg
      361 tgggagaaag tcgataaatc aaatcgtacc agagcctctt ctacaatggc attatcgtgc
      421 gaagtgattc tgccttgatg atgagggtaa cgcccaaata acagcagatc tttgacggta
      481 atacggctat gaatcacatt atcttgggtc aagatagcca gttgtcgagc gatagtttga
      541 gaagctgtgt tggctatatt cagctcattt aagtggattt caccctgttg gataggttgc
      601 aagcgtgcaa ttaaagagag cagggtggat ttgcccgcac catttgctcc gattaaagcg
      661 gtaataccac catttggaat ggttaaattg atattattca gaatcgtggc attcccgatt
      721 ttgtggtgaa gatttttaac cgttatcatt ttcttcctta atttatggtt attttttccg
      781 ttttaaaatg agataaataa agacaatacc gccaataaat tcaataacga cactcagaac
      841 cccttgcatt ttcagcactt gctcgaagat tgcctgccct aaaattaagg tgatggcaga
      901 gattaaaaaa gtcattggta ttcgcacgct atggcaaaca gtcgggctaa ttgcattaac
      961 aatggcacaa acaagtagcc ctagaaatag aatcgggcca acaagtgcgg ttgaaacaga
     1021 caccaacaat ccacaacaga ttagtaattg acgtgaaaat tgatgatatt ctaacccaag
     1081 cccaattgcc ttatctttgc caagtaacaa aatatctaat ttatgccgtt gtaaccagac
     1141 ccaaatggca gagagaatag cgatacagcc tccaatccaa agtaatgtgg gggtagtcag
     1201 gttaaatgag gcaaagaaga gccttgagca acggcaaact ctgttgggtc gacattcgtt
     1261 gcaacaaatt atttaggctt ctaaataaga caccaaagat gacaccgatt aaaagcattc
     1321 gggttaaatc ggatcgattt cgattcagtg aactaaatag taatattgat gcccctagca
     1381 ttaacaaggt ttcaaagaaa aacttatagc ttgggtctaa ctgagtaaag cctaaaccgc
     1441 caaataaaaa tactaacgag gtttgcaata gcaagtagag cgaatcgaac cccaaaatac
     1501 ttggagttaa aataggatta tgtgtgagtg tttggaatag cagggtcgaa ataccaatcg
     1561 tataagcaat agtacagagc aaaataagct ttttgccacg cagttgcaat ataaagtccc
     1621 aattaccttt cgcattatag gtcatataaa acagaatact tgctaataag attaagctta
     1681 aaacgataaa gcgtgatcta accacgagcc ttctcctcag cccgtttctc tttgagcaat
     1741 aaatagagga aaatcactgt cccaataatc ccaaaaatag tagaaatcgg cacttcataa
     1801 ggggcattga tggctcgccc taaaatgtca caaatcagga ctaaattcgc acctaacacc
     1861 acgaccgcag gcagattttt acgcaatcgg tcacctgcta tgcgagagac aatattaggg
     1921 acgaccaagc cgataaacgg aatctgtcca accgtcacca cgacaatagc ggtaatcatc
     1981 gccaccacaa ttaaggcaag ccaagtcatt tgtttgtagt taatgcctaa gttggtacta
     2041 atattttcac caaggcctgc aatggttaat tgatctgcca tcacataaac cacaacagcc
     2101 agaatagcgg taatccagag cagttcatac cgacctgcca aaataccgga gaagtcgccg
     2161 gaaaaccaaa tcgaaagcac ttgtaggctg tcagtttcat aagcaataaa agtgctgata
     2221 gcttcaataa cattgccaaa aacgatcccg attaatggga gcattaattg ttgatgaggc
     2281 ggtaattgac gaatgagcaa cataaaaagc cccattccca ttaatgcact taatgtggca
     2341 atagacattt ttaacagtaa tgctgatgtt gggaaaagta aactagcaag taaaatacca
     2401 attgccgcac tttggctagt gccaatcata ctaggctcaa taaagcggtt cttcagcaca
     2461 atttgtaata ccatacctgc aacggctaag gttgcaccga ctaatatcac tgcgagcgtt
     2521 cttggtaaac ggctgataaa aaagatctga ctttgttcgg aagaatgaaa aatttcactc
     2581 caatgaaaat ctgccacacc cacactggca ctgattggaa tgagcaatat taaaagaaat
     2641 agatttaatc taaaaaaaga gaacatagtg ataaatagca agcggtcata ttctctctct
     2701 tttttacaaa tgcaggcact tgcaaaaata ggaggaaatc tgaccgcttg taaggttaat
     2761 tattttttct caaatgctgt tttaatgtta gttaggtcgg ttttcatctg ctcataacca
     2821 cccggcgcta aatatgaggc agcacttaga taaacgatat taccgttttt ccaagcttta
     2881 gtttgacgaa ctaattcatt atccaacact tgttgggctg attgaccttc ttgtccgata
     2941 gccgcaatac ggtctaacac aaacaaccaa tccggattga gtttttggat aaattcaaac
     3001 gaaacaggct gaccgtgacc ggtattttta atgctagaat ctgccattgg aatccctaaa
     3061 ttatcgtgaa tccaacttaa acgataacct gaaccaaatg cagaaatttt gccaccattg
     3121 actaaaataa tcagtccgtt acctttacct tttactgctg ctttggtttg gtctaataaa
     3181 gtattaattt ctgcttttag tttttcggtt tccgcttctt tattaaataa tttgccgaaa
     3241 gaatcaagac gctgtaatcc gctttcgatt aatttatcgc cattatccgt catgtcgatg
     3301 gttttcgcaa tttggctgac ttcatcaaat ttttttgctg tgcgtgtgcc aacaatgatc
     3361 aaatccggat ttaaagaatt taacgcttct aaatccggct caaataccgt accgacattc
     3421 accgctttgt ctgcactcgg tttaagataa gaaagcacct taaattcagg cataccgtca
     3481 atttttacac ctaaggcttg taaagtatcc aaacttccgg tgtcaaacac ggcaatttta
     3541 gacggggttt ctttgaaagt aacctctcca cgtgcagttg gaatagtaac atctttggca
     3601 aatgcagaag cagcaaacac agcagttgcg gtcaatgcaa acaaggcttt tttaatcatt
     3661 ggataactcc atttatattg aaaataattc tcaaagattt taacgaaaat tgattttaag
     3721 taaagcggaa aataacaagc gggcgaattt tgcctaaaat ttgcaaattt caatttaatt
     3781 aaaattccga tacactatgt acatcaatac gtcattatag caagagaaaa atatggcaga
     3841 atatagcgga acaacccaag agaagatgaa agataagcag gttgagcaaa ttaatatctc
     3901 accacattcg attgaggcag agcaagcggt gcttggtggc attatgctca gtaatgatca
     3961 ctgggataat gttgctgagc gtgtacaacc gcacgatttt tacaactatg cgcatcgaat
     4021 tatttttgaa caaatggcgg agttggtgcg ccacaataaa ccgattgata ttattacgct
     4081 ggatcaagcc ttaaaagaca aaggggtgct gcaagatgta ggaggttttg cctatttagc
     4141 cgagctttct aaaaataccc cgagtgcagc aaatatttta gcctatgcgg atattgtgcg
     4201 agatcgttcc gatttaagaa gtgtaatcag tgccggcaat caaattgcgg aaatgggcta
     4261 caaaacgaaa gggcgtaact ccaaagaagt gttagacgag gcagagcgaa ttgtgtttga
     4321 aattgcagaa aaacgcaata acgccaatga aggtccacaa cgtattgacg atattttgct
     4381 taaaacactc gctcgaatgg atacgctatc taaaaatcgc cataacggcg gagttactgg
     4441 tgtttctacc gggtttgttg atctcaataa aaaaaccgca gggttacagc cttcggattt
     4501 aatcattgtg gcagcccgtc cttcaatggg gaaaactact tttgcaatga acttatgtga
     4561 aaatgcctca ttagtggata ttgaagataa agatgattta gacgaacagg gtaatccgaa
     4621 gaaaaaaccg gcaaagccag tgctaatttt cagcctggag atgccggcag accaaattat
     4681 gatgcgtatg cttgcctcac tttctcgggt cgatcaaacc aagatccgaa ccggtcagat
     4741 ttcagatgat gaagacactg ctaaaatctc cagcacaatg gcaattttac aagaacgtaa
     4801 taatatctat attgatgata gctccggttt aacaccgacc gaattacgtt ctcgagcgag
     4861 acggatttat cgtgaaaatg gcggattaag cttgattatg gtggactatc tccaattaat
     4921 gcgagcgccg ggatttgcag ataatcgtac tttagaaatc gcagaaattt cccgttcttt
     4981 aaaggcacta gcgaaagaat tagaagtgcc agtagtcgct ctttctcagc taaaccggag
     5041 tttggaacaa cgtgccgata aacgtccggt aaactcggat ttgcgtgagt caggttctat
     5101 tgagcaagat gcagacttaa ttatgtttat ttatcgtgat gaggtctata acgacaattc
     5161 cgatctgaaa ggcattgcgg aaattattat cggaaaacaa cgtaacggcc cgattggtcg
     5221 ggtgcgttta acgttccaag ggcagtattc ccgttttgat aattacacag gagggcatta
     5281 tgatgatgaa tactaaacca acccagccaa tcacactttg tgcgatttat cgcagtaagg
     5341 caaaagaagg aatgtattta tatgtgccta agcgtgacca atttgagcaa gtgccggaaa
     5401 cattacgcca gttatttggt aagcctgaat ttgtgatgat gtttaattta accggtgaaa
     5461 agccattggt gcgtgctaaa aacgaagaag tgttacaatt acttatagag caaggctact
     5521 atttgcaaat gccacctccg ccggagaatt tgcataaagc ctttatcgca caacagaaag
     5581 gggaaaaata atgcgctgcc cattttgttc cactgaagaa actaaagtga tcgattcacg
     5641 gcttgcagca agcggttatc aaattcgccg ccgccgtgaa tgtataagct gtaaagaacg
     5701 ttttactacc tttgaatcgg ccgaattagt tgtgccttat gtgatcaaaa ataatggtag
     5761 ccgagagccg tttgatgccg aaaaattaag aacagggtta agacgtgcat taaataaacg
     5821 ccctgtcagt gctgatgatg tcgaaaaagc aattagccaa atcattattc agctgcaagc
     5881 gatgggcgaa cgagaagtgc cgagtcgcca tatcggtaca ttggcaatga acgcacttaa
     5941 acaattagac aaagtagcat atatccgctt tgcctctatt tatttaagct ttagtgatat
     6001 tgaagagttc attaaagaaa ttgaaaaatt aaaagagtag ttgcaaaaaa acataaaaaa
     6061 atgaccgctt gtgagtttaa aatccacaag cggtcatttt tctttaaaaa tactcaatta
     6121 gcttttactt gttccaacac aaaccctaca aacgcccgca caatgctaga cggataacga
     6181 tgggcggcat aaatcatcgt caaatcaaat ttggggtttt ctgtctcacc caagacttcc
     6241 accaagccat tttctgccac caatctatcc accgtgctgt cggcaagcag agccactgct
     6301 ttttgccata tattcaatgg catagctgtc attgctggaa aaaagatagg gaatttctac
     6361 cgcttgtctg ccagtgccta gcgtgatgac gcttgcttca ttttttgccc aagcggcaat
     6421 ggggaaattt ttcaaatctt tgacggtgct gggcgtgcca aagcgtgcca aaaatttagg
     6481 gtgtgccacc catttactgc cgatttgccc cacttttttg gcgataaact gttcgccgtg
     6541 tagttgccca atccgaaacg ccacatcaat gccatctgcc gacaaatcca aaagacggtc
     6601 ggtcagcgtg caatgcactt gtacttgtgg ataagctgcc tgaaaatcag ctatccacgc
     6661 caaaacaggc tcgcaggcag ggcttgccga caggcgtaat ttacccttta aattgccttg
     6721 ctcttcaaac aacacttctt tggcagtggc aagctgttca atggacaaat gcacctgttc
     6781 atacaggcgt tgccctgcca ttgtgggttt aacgcctgat ttttggcggt caaagagttg
     6841 aatgttaagc tctttttcca aatcggcaat ttgacggcta atggttgcaa tcggcacatt
     6901 gagtcgctcg gaggctttgg agagactgcc gtgctggata acggcgacaa agaggcggag
     6961 ggtgttgagg ttcattgttt ttctccccaa tgttcaattt cttgaataaa atgtttaata
     7021 ctgccttgga cttgttgatt tttaccatat agataataaa cgcctgaaag ttcttgtaaa
     7081 aatttacttt ctttataagc gtgtttt
//
LOCUS       DANLXF010000124         7100 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.124, whole genome shotgun sequence.
ACCESSION   DANLXF010000124 DANLXF010000000
VERSION     DANLXF010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7100
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.124"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            137..1399
                     /locus_tag="PXD66_001649"
     CDS             137..1399
                     /locus_tag="PXD66_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDL6319933.1"
                     /translation="MGTLNSFLGIIVLLFIAFLFSSNKRAINVRTVLGALALQVAIGA
                     LVLYVPAGRDALNAMATGVSKVISYGNEGISFLFGGLVSDKMFEVFGGGGFVFAFRVL
                     PVIIFFSALISLLYYIGVMQWVIRILGGLLQKALGTSKAESMSAAANIFVGQTEAPLV
                     VKPYISKMTESELFAIMAGGLASIAGSVMAGYAGMGVPLTYLIAASFMAAPAGLLFAK
                     IMVPQTEKFRDELEAVDLEKPANILDAAASGASSGMQLALNVGAMLIAFVALIALVNG
                     ILGGIGGWFGYDTLNLGQIFGWVFKPLAWVIGVPWEEAEIAGQMIGLKLAVNEFVGYL
                     EFAKYLAPESTVHLTDKTVAIITFALCGFANFSSIAILIGGIGAMAPSRRGDIARLGI
                     KAVIAGSLANLMSATIAGLFIGLGGAVL"
     gene            1544..2320
                     /gene="deoC"
                     /locus_tag="PXD66_001650"
     CDS             1544..2320
                     /gene="deoC"
                     /locus_tag="PXD66_001650"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDL6319934.1"
                     /translation="MNLKKSAEIALSLMDLTTLNDNDTDEKVIALCQQANTKFGTPAA
                     ICIYPRFIPIARKALKQQGIEQVKIATVTNFPHGNDDIEIAVAETKAAVAYGADEVDV
                     VFPYKALIAGNEQIGFDLVKQCKAVCAANNVLLKVIIETGELKSAELIRKASEISIQA
                     GADFIKTSTGKVAVNATLESARIMLETIRDLGVADRVGFKAAGGVKTSEEAAQYLALA
                     ESILGNDWVSASHFRFGASSLLANLLATLNDEQQQAVQGY"
     gene            2341..3060
                     /gene="deoD"
                     /locus_tag="PXD66_001651"
     CDS             2341..3060
                     /gene="deoD"
                     /locus_tag="PXD66_001651"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDL6319935.1"
                     /translation="MATPHINAPEGAFADVVIMPGDPLRAKYIAETFLEDAKEVTNVR
                     NMLGYTGTYKGRRISVMGHGMGIPSCSIYAKELITEYGVKKIIRVGSCGAVRNDVKVR
                     DIVIGVGACTDSKVNRIRFRDNDFAAIADFDMTLAAHQAAKAKGIEARFGNLFSADLF
                     YSPDVEMFDVMEKYGILGVEMEAAGIYAVAAEYGAKALAICTVSDHIRTGEQTTSEER
                     QLTFNGMIEIALESVLIGDKQ"
     gene            complement(3136..3987)
                     /locus_tag="PXD66_001652"
     CDS             complement(3136..3987)
                     /locus_tag="PXD66_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDL6319936.1"
                     /translation="MQTHRAIIESIFALAPKNELWATPISGLTVHAADRSIRFNGYIQ
                     DPSLCVVLLGERQICTNGHCQVFGNQEMMFCPVNLPIVTQTLSVAENQPYLAISLRLD
                     FDLIAKIITQLPPQKISENINQRTKWELENEIKDCLLRLLNLLNSPNHIHFLAPLILN
                     ELYFYLLQSEQGGYIRSLLSQDGNIAKIANATKILERKFADTLNMSALAEEVGMSVAG
                     FYAHFKSVTSMTPLQYQKSLRLNAAREKLQAGVSVSETAYQVGYESPSQFSREYKRQF
                     GESPKGR"
     gene            4102..4533
                     /locus_tag="PXD66_001653"
     CDS             4102..4533
                     /locus_tag="PXD66_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDL6319937.1"
                     /translation="MKIFYHTSATATGGRDGRTQVDDGSIGFDLVGFQNESGKVGTNP
                     EQLFAMGYAACFDSAMNHVAPTLGIKPTKSSTTVAVGIGQKADGAFGLDLDITITVEG
                     ISAEEAKTLISKAHEVCPYSNATRGNVDVRLHVKVVSEFDL"
     gene            4550..5230
                     /locus_tag="PXD66_001654"
     CDS             4550..5230
                     /locus_tag="PXD66_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HDL6319938.1"
                     /translation="MKMLDFETTVKTRQSIRKFLPLPMTETEIKQILADAQNAPSACN
                     TQPWLVHVASGETLARLKARFKKTFEAGINDADFEFNQTKFTGDYEPRWRNQYSHVFT
                     TSYGIPREDKAGRQVVYDDNIVGYGAPHMAFLFMPSIDGHDVNIASDVGMYSQTFLLS
                     LTARGFGGIPQLALAMFAKDVREELGIPEHYKLLHAISFGHPDWDAMQNKHHLGRVPV
                     EESATLHW"
     gene            5294..6418
                     /locus_tag="PXD66_001655"
     CDS             5294..6418
                     /locus_tag="PXD66_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDL6319939.1"
                     /translation="MINLFTPYTLNNDVEIKNRLVVAPMTHSASNDDGTISEQERHFL
                     HNRAADMGLFITAATGVMRNGKAFYGQPEALDERYLASLTETANILKNQGTKAILQIF
                     HGGAQAIPALLDGANAVAPSEHANGAKALTADEVKQMIQAFANATDLAIQAGFDGVEI
                     HGANGYLIQQFFSTEFNQRTDEWGGSLEKRLRFPLAVVEAAVSVREKHQRPDFIIGYR
                     FSPEEPGEHGLTMTDTYALIDALCEKPLQYLHISLWDFGKKARRGADTNFTRMQLVHQ
                     HINGRLPLIGVGNLLTGEQIRKAYATGWAEFIALGKAVMLNPNVATLLKDNRDSEIVS
                     ELDPQQADHYGIPDRLWGYCVQGGAWLPPLKGQNGQPMDI"
     gene            6502..>7100
                     /locus_tag="PXD66_001656"
     CDS             6502..>7100
                     /locus_tag="PXD66_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HDL6319940.1"
                     /translation="MITLHYLNSSCSHRIVWLLEALGLDYELKIYHREESGFAPMEVK
                     ALHPLGKAPVLQDGDLVLAEGNAIIQHLIARYAEGKFEPTRGSEAYSMYHYWLSISAS
                     LFSANLVTMLANRSDLGAFGDYAKAQTPLYFNHIEQTLSDKKWIVGDELTGADFSLCF
                     PLQWGRNFVEMANYPNIARYLEQVENHPAYQKANQKALKL"
BASE COUNT         2003 a         1424 c         1612 g         2061 t
ORIGIN      
        1 tgtgatgaat aataaaatac cataatttag tttttgtcaa attttgtctt gaatgatgat
       61 aatttcacaa gattttttgc ctaaaaatag gtatagtatc gtcacctttt ttaatcaatt
      121 tataattaga ggttctatgg gtactcttaa cagcttttta ggcattattg ttttactatt
      181 cattgcattt ttattttcga gcaataaacg tgcaataaac gtgcgtaccg tattaggcgc
      241 attagcattg caggtagcta ttggtgcatt ggtgctttat gtgccggcag ggcgtgatgc
      301 cttgaatgcg atggcaacag gtgtgtcaaa agtgatttct tacggtaatg aagggatttc
      361 atttttattc ggtggcttag tcagcgacaa aatgtttgaa gtatttggcg gcggcggctt
      421 tgtgttcgct ttccgtgtac ttccggtgat tattttcttc tctgcactca tttctttgct
      481 ttactacatt ggagtgatgc aatgggtaat ccgtatttta ggtggtttat tacaaaaagc
      541 attaggtaca tctaaagcgg aatctatgtc tgcggcagct aatattttcg ttggccaaac
      601 tgaagctcca ttggtagtaa aaccttatat cagcaaaatg acagaatctg aactctttgc
      661 gattatggca gggggcttag cttctattgc aggttctgtt atggctggtt acgcaggaat
      721 gggcgtacca ttaacgtact taatcgcagc ctcctttatg gcggctccgg cagggttatt
      781 attcgccaaa attatggtgc cacaaaccga aaaattccgt gatgaactag aagcggtaga
      841 tttagaaaaa ccggcaaata ttcttgatgc agcagcttca ggtgcttctt caggtatgca
      901 actggcatta aacgtagggg caatgttaat tgcattcgtg gcgttaattg cattagtgaa
      961 tggtatttta ggaggtattg gcggctggtt cggttatgat accttaaact taggtcaaat
     1021 tttcggctgg gtatttaaac cgcttgcgtg ggtaatcggt gtgccttggg aagaagcaga
     1081 aattgcgggg cagatgatcg gcttaaaatt agcggtaaat gaattcgtgg gttatttaga
     1141 gtttgctaaa tacttagctc ctgagtcaac cgtacattta acggataaaa ctgttgcgat
     1201 tattaccttt gcactttgtg gctttgcgaa cttcagctcg attgcgattt taatcggtgg
     1261 tatcggggca atggctccaa gccgtcgtgg tgatattgcc cgacttggga ttaaagccgt
     1321 cattgctggc tcacttgcca acttaatgag tgcaaccatt gccggcttgt ttatcggctt
     1381 gggtggagca gttctctaat ccagtttgta taaaaccact agaaatagtg gttttatctt
     1441 tttttatctg acgtaaacgg ttgcttttgg tttgtctatt tacaagcggt cagattttta
     1501 taaaatttta ccaaccacca cgcatttaat aaaaggagac actatgaatt taaaaaaatc
     1561 cgctgagatt gctctttcgt tgatggatct caccacatta aatgacaatg atactgatga
     1621 gaaagtcatc gcattatgcc aacaagcgaa caccaagttt ggcacaccgg cagcaatttg
     1681 catttatcca cgttttatcc cgattgcccg caaagcattg aaacagcaag gtatcgagca
     1741 agtaaaaatt gcgacagtca cgaatttccc tcacggtaat gatgatattg aaattgcggt
     1801 agctgagacc aaagccgcag ttgcttatgg ggcagatgag gtagatgtgg tattccctta
     1861 taaagcatta attgcaggta atgagcaaat aggtttcgac ttggtaaaac aatgcaaagc
     1921 agtttgtgct gccaataatg tgttgcttaa agtgattatt gaaaccggcg agttaaaatc
     1981 agcagagctt attcgcaaag caagcgaaat ttcgattcaa gcaggggctg attttattaa
     2041 aacctcaacc ggcaaggtgg cagtgaatgc tacccttgaa tctgcccgca ttatgcttga
     2101 aaccattcgt gatttaggtg tggcagaccg tgttggcttt aaagctgcag gtggggtaaa
     2161 aacctcggaa gaagccgccc aatatcttgc acttgcggaa tctattttag gcaatgactg
     2221 ggtgagtgca tcacattttc gcttcggtgc atcaagttta ttagctaatt tattagcaac
     2281 attgaatgat gaacagcagc aagctgttca aggttattaa ttaataaaga ggaacataaa
     2341 atggctactc cacacattaa cgcacctgaa ggtgctttcg ctgacgttgt aattatgccg
     2401 ggcgatccgc ttcgtgctaa atatatcgct gaaacctttt tagaagatgc aaaagaagtg
     2461 accaatgttc gcaatatgtt aggctacacc ggcacttata aaggtcgccg catttcagta
     2521 atggggcacg gtatggggat tccatcttgc tcgatttacg ctaaagaatt aattactgaa
     2581 tatggcgtga agaaaattat ccgtgtcggc tcgtgcggtg cggtacgtaa tgatgtcaaa
     2641 gtgcgtgata ttgtcatcgg tgttggggct tgtaccgatt ctaaagtaaa ccgtattcgc
     2701 ttccgtgata acgattttgc ggcgattgct gattttgata tgacattagc tgctcaccaa
     2761 gcagctaaag cgaaaggcat tgaagctcgt ttcggtaatt tattctcagc cgatttattc
     2821 tactcacctg atgtcgaaat gtttgatgta atggaaaaat acggcatttt aggcgtagaa
     2881 atggaagcag caggcattta tgcggtagcg gcagagtatg gagcaaaagc attagcaatt
     2941 tgcaccgttt cagaccatat ccgcaccggt gagcaaacta cctctgaaga acgtcaatta
     3001 acctttaatg gtatgattga aattgcgtta gagtcagtat taattggcga taagcagtaa
     3061 tttctaacct gacaagcggt gaaattttgc aaaaaatttg cagaatttga ccgcttgtta
     3121 tttttgattc ttcctttatc ttcctttcgg gctttcgcca aactgccttt tatattctcg
     3181 actaaactgg cttgggcttt catagccgac ttgataggcg gtttcactca cgcttactcc
     3241 tgcttgtagt ttttcccttg ccgcatttag tcttaacgat ttctgatatt gcagtggtgt
     3301 cattgaagtt acgcttttaa aatgagcgta aaaccccgcc acgctcattc ctacctcttc
     3361 agccagtgct gacatattta gtgtatcggc aaattttctt tctaagattt ttgttgcatt
     3421 ggcaatttta gcaatattgc catcttgaga gagtagagag cgtatatagc caccttgctc
     3481 agattgtaat aaatagaaat agagttcgtt taatatcaat ggtgcaagaa aatgaatatg
     3541 atttggagaa ttaagcaaat ttagtaagcg taacaaacag tctttaattt cattctccag
     3601 ctcccattta gtgcgttgat taatgttttc acttattttc tgtgggggaa gctgcgtgat
     3661 aattttggca ataagatcaa aatccaagcg tagtgaaatc gcaagataag gctgattttc
     3721 tgcaactgat aaggtttggg taacaatcgg caaattcaca gggcaaaaca tcatttcctg
     3781 attaccgaag acttggcaat gtccattggt acagatttgc cgctcgccta ataatactac
     3841 acataaactt gggtcttgga tataaccgtt aaatctaata gaacgatcgg cagcgtgaac
     3901 ggttagccct gaaatgggag tagcccaaag ttcatttttc ggagcgaggg caaaaataga
     3961 ttcaataata gctcgatgag tttgcataat aatctcactt ttagaaaatt aggcatattt
     4021 tatagaaaat caggcaagac gacaaatagc aaaaagcgga taatatcccc aatcaaataa
     4081 agcatttaat aggagcaaaa tatgaaaatt ttttatcaca catcagcaac tgcaaccggt
     4141 ggtcgtgacg gcagaaccca agtggatgac ggttctatcg gttttgattt agtcggtttc
     4201 caaaatgaaa gtggcaaagt aggcactaat ccggaacaat tattcgcaat gggctatgct
     4261 gcctgctttg acagtgcaat gaatcacgtt gccccaacct taggcattaa gccaaccaaa
     4321 tccagtacca ctgttgcagt tgggattggt caaaaagctg atggtgcttt cggcttagat
     4381 ttagatatta ccattactgt ggaaggcatt agtgcggaag aagcgaaaac tttaattagc
     4441 aaggcacacg aagtttgccc atattcaaat gcaacccgtg gtaatgtaga tgtgcgttta
     4501 cacgttaaag tcgtgagtga atttgactta taaaaaataa ataggggaaa tgaaaatgtt
     4561 agattttgaa accacggtaa aaacacgaca atcaatccgc aagtttttac cgcttccgat
     4621 gacggaaact gaaatcaaac aaattcttgc cgatgcacaa aatgcccctt cggcttgtaa
     4681 tacacagccg tggttagtac acgttgcctc tggtgaaacg ctagcacgct taaaagctcg
     4741 ttttaaaaag acttttgaag cgggcattaa tgatgcggat tttgagttca atcaaacgaa
     4801 atttaccggt gactacgaac cacgttggcg taaccaatac agccacgttt ttaccaccag
     4861 ctatggcatt ccccgtgaag ataaagccgg tcgccaagtg gtgtatgatg acaatattgt
     4921 cggttatggt gcgccacata tggcattcct atttatgcca agcattgacg gacacgatgt
     4981 caatattgcc agcgatgttg gtatgtattc gcaaactttc ttgctctcac tcaccgctcg
     5041 tggttttggc ggcattccgc agcttgccct tgcgatgttt gctaaagatg tgcgtgaaga
     5101 gttaggcatt cctgaacatt acaagctcct ccacgccatt tctttcggac acccagattg
     5161 ggacgctatg cagaacaaac accatttagg gcgtgtgccg gtggaagaga gtgcgacctt
     5221 acattggtaa taaacaagcg gttgattttt cgcaaaaatt tgcgaaatca accgtatttt
     5281 ttaggagaat acgatgataa atttatttac cccttatact ctgaataacg atgtagaaat
     5341 taaaaaccgc cttgtagttg ccccaatgac ccattccgcc tccaacgatg acggcacaat
     5401 tagcgagcaa gaacgccatt tcttgcataa tcgtgcagca gatatgggct tatttatcac
     5461 cgcagcaacc ggggtaatgc gtaacggcaa agcgttttac ggtcagccgg aagctcttga
     5521 tgaacgctat ttggcaagcc tgacagaaac tgccaatatc ttgaaaaatc aaggcaccaa
     5581 agcgattttg caaattttcc acggtggggc acaagctatt cctgccttgc ttgacggggc
     5641 gaatgcagtt gcgccgtccg agcacgccaa cggtgctaaa gccctaacgg cggacgaagt
     5701 gaagcaaatg atccaagctt tcgctaatgc caccgattta gctattcaag ccggttttga
     5761 tggtgtggaa attcacgggg cgaacggcta tttaattcaa caatttttct ctactgaatt
     5821 taatcaacgc actgatgaat ggggcggtag ccttgaaaaa cgcttgcgtt tcccgttagc
     5881 ggtggttgag gcagcggtca gcgtgcgtga aaaacaccaa cgccctgatt ttattatcgg
     5941 ctaccgtttc tcaccggaag agccgggcga acacggctta accatgaccg atacctacgc
     6001 cctgattgat gctttgtgcg aaaagccgtt gcaatacttg cacatttcac tctgggattt
     6061 cggcaaaaaa gcccgccgtg gtgcagatac caacttcacc cgtatgcaac tggtacacca
     6121 acacatcaac gggcgtttac cgctgatcgg cgtgggtaac ttattaaccg gtgaacagat
     6181 ccgtaaagct tatgcaacag gctgggcaga atttatcgcc ttaggtaaag cggtaatgct
     6241 caacccgaac gtggcgacct tgttgaaaga taatcgagac agtgagattg tcagcgaact
     6301 cgacccgcaa caagccgacc actacggcat tcccgataga ctatggggct actgcgtgca
     6361 aggtggtgct tggttaccgc cgcttaaagg gcaaaacggg caaccaatgg atatttaatc
     6421 attacagaca agcggtcatt tttttgtaaa aatttgcaat taagtgaccg cattttttca
     6481 attatctgaa ggagaaaaat tatgattacg ttacattacc tgaacagttc ctgctctcac
     6541 cgcatcgttt ggttgttgga agccttaggc ttggattacg agctaaaaat ctaccatcgt
     6601 gaagagagcg gttttgcacc tatggaggta aaagccttgc acccacttgg caaagcccct
     6661 gtgttgcaag atggcgattt ggtgcttgcc gaaggcaatg cgattatcca gcatctgatt
     6721 gcccgttacg ccgaagggaa atttgagccg actcgtggta gcgaagccta ttcaatgtat
     6781 cactactggt tgtcgatttc tgcttcatta ttctcggcaa accttgtgac aatgttggct
     6841 aatcgctctg atctcggagc ttttggcgac tacgcaaaag cgcagactcc gctgtatttt
     6901 aaccatattg agcaaacact ttcagataaa aaatggattg tgggtgatga actaaccggt
     6961 gcggattttt ctctctgttt cccactacaa tgggggcgta attttgttga aatggcaaat
     7021 tacccgaata ttgcacgcta tttagaacag gtggaaaatc accctgctta ccaaaaagcg
     7081 aatcaaaaag ccctgaagct
//
LOCUS       DANLXF010000125         7021 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.125, whole genome shotgun sequence.
ACCESSION   DANLXF010000125 DANLXF010000000
VERSION     DANLXF010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7021
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.125"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..767)
                     /gene="znuB"
                     /locus_tag="PXD66_001657"
     CDS             complement(<1..767)
                     /gene="znuB"
                     /locus_tag="PXD66_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HDL6319941.1"
                     /translation="MFDILFPAWLAGVLLAFITAPLGAFVVWRKMAYFGDTLSHSALL
                     GVALGIFLELDPYLAVIGMTILLALLLVWLEQKTNYSVDTVLGIIAHTSLSLGVITIS
                     LLDNVRVDLMSYLFGDLLAINFSDVVFIGIGATLICLILMLFWKRLLSITISPELAQI
                     EGLNVARLRLLLMILTALTIALSMKFVGALIITSLLIIPSATARRFAKTPEKMVLIAI
                     FFSIIAVSGGLLLSSWKDTPAGPSVVICAGLIFLLSL"
     gene            complement(854..2191)
                     /locus_tag="PXD66_001658"
     CDS             complement(854..2191)
                     /locus_tag="PXD66_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDL6319942.1"
                     /translation="MSSLSFDFSEDFRPLAAKMRPRNLAEYVGQSHLIGEGKPLRRAI
                     EAGHSHSMIFWGPPGTGKTTLAEIIAHHLDAEVERISAVTSGVKEIREVIEQAKLNRQ
                     AGRRTLLFVDEVHRFNKSQQDAFLPHIEDGTIIFIGATTENPSFELNNALLSRARIYI
                     LKPLQAVEILKILQMAIADTERGLGNETLVLEDDVLALLADYVNGDARFALNCLELMV
                     DMAQDSAKGKVLNKSLLTEVLGERQARFDKGGDRYYDLISALHKSIRGSSADGALYWY
                     ARILTAGGDPLYVARRLLAIASEDIGNADPRAMQIAIVAWDCFTRVGAYEGERAIAQA
                     IIYLAVAPKSNAVYQAFNEAKRLAKEAKDYDVPAHLRNAPTQLMKSLGYGEEYRYAHN
                     EPNAYAAGENYFPPELKDTEFYFPSERGMEKQIKEKMAWLKAQDQASPIQRYK"
     gene            complement(2201..2689)
                     /gene="mreD"
                     /locus_tag="PXD66_001659"
     CDS             complement(2201..2689)
                     /gene="mreD"
                     /locus_tag="PXD66_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDL6319943.1"
                     /translation="MRTNPIFQILVIIAIFVVAFVLEIMPWPVGFQGLRPTWVVLVLI
                     YWALALPDKMSVGTAFIAGIVWDLILGSILGIHALVLSVAIYFVAKYHLILRNLSLWL
                     QSLLVMAYIVLIRFFIFVIEYILHSAEFNSQELLGAVISGVLWSWIFLLMRHIRRQLR
                     LR"
     gene            complement(2689..3744)
                     /gene="mreC"
                     /locus_tag="PXD66_001660"
     CDS             complement(2689..3744)
                     /gene="mreC"
                     /locus_tag="PXD66_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDL6319944.1"
                     /translation="MKPIFAKAPSLGMRLIIAVIFSIMLIISDGRSSLMIQARNLLET
                     AVSGLYYFANTPRSILDGVSGNFIDNNKLQLENNLLKEQLREKSADLLLLDQLKVENQ
                     RLRLLLSSPLRQDEYKKIAEVLAAEMDAYRQQVVINQGRLDGAFVGQPIIDERGVVGQ
                     VISVGEKSSRVLLLSDITHAIPVQVLRNDVRGIASGTGVNNELVIDNLPRSIDVVKGD
                     VLVTSGLGGRFPEGYPVAIVETVENDTKSQFARIVARPLASFDRLRYLLLLWPTAEEK
                     NYSQSLSPAQVREVVEERRNSVNPFERLKQMSNKKTEVQLEAKEEQIENDEINENPAA
                     QPEAENEQNYHTEEGAE"
     gene            complement(3846..4898)
                     /locus_tag="PXD66_001661"
     CDS             complement(3846..4898)
                     /locus_tag="PXD66_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HDL6319945.1"
                     /translation="MFKKFLGLFSNDLSIDLGTANTLVYVKGQGIVLDEPSVVAVRQD
                     RMGSLKSIAAVGSHAKLMLGRTPKSIMAIRPMKDGVIADFFVTEKMLQHFIKQVHSNN
                     FMRPSPRVLVCVPAGATQVERRAIKESAIGAGAREVYLIEEPMAAAIGAGLPVHEAMG
                     SMVIDIGGGTTEVAVLSLNGIVYSTSVRIGGDKLDEAIIAYVRRHFGSAIGEATAERI
                     KQEIASAKIESDDDVKTMEVHGHNLAEGAPRTFELNSRHVLEAIEQPLNGIVEAVKSV
                     LEQCPPELAADIFERGMVLTGGGALLHNLDRLLSDSTGVQVVVAEDPLTCVARGGGKA
                     LEMIDMHGGDVFSIDD"
     gene            complement(5013..5642)
                     /locus_tag="PXD66_001662"
     CDS             complement(5013..5642)
                     /locus_tag="PXD66_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HDL6319946.1"
                     /translation="MDIFIQGFIVCFGLIVSIGAQNAFLLKQGILKQHIFWIALLCFL
                     GDVFLMTIGVLGLGTLIAELPVVSFIISLLGAIFLLSYGSRSFISVFKSGEYLVASGE
                     NATSLKKALLITFAITFLNPHVYIDTVVIVGSIGGKLDFNGKIYFLAGALLCSFIWFF
                     GIGYGASLLSPYFAKRRTWQILDAVTGLIMYFIAFSLLIYTYQLALQIF"
     gene            5746..6393
                     /gene="udk"
                     /locus_tag="PXD66_001663"
     CDS             5746..6393
                     /gene="udk"
                     /locus_tag="PXD66_001663"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDL6319947.1"
                     /translation="MTDTSQTSSCVVIAIAGASASGKSLIASTIYKELKNELGTDNIG
                     IISEDAYYRDQSHLTMEERIKTNYDHPNSMDHHLLVEHLRQLKQGNAIEIPEYDYTEH
                     NRKTTTKHFEPKKIIILEGILLLTDENIRNEINVSIFVDAPLDICFIRRLQRDMVERG
                     RSMESVISQYRKTVRPMFLQFIEPSKQYADIIVPKGGKNRIAIDILKAQIKQLLK"
     gene            6408..6989
                     /gene="dcd"
                     /locus_tag="PXD66_001664"
     CDS             6408..6989
                     /gene="dcd"
                     /locus_tag="PXD66_001664"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HDL6319948.1"
                     /translation="MRLCDTDIKRYLDEGIIAITPRPSDDKISGATADVRLGNSFRVF
                     REHKTPYIDLSGPREEVAAQLNKVMSDEIIIGDDEAFFLHPGDLALATTLESVSLPAN
                     IVGWLDGRSSLARLGLMVHVTAHRIDPGWEGKIVLEFFNAGKLPLALRPNMAIGALSF
                     EILSGDAAKPYNARKDAKYKNQQSAISSRINQD"
BASE COUNT         2266 a         1575 c         1228 g         1952 t
ORIGIN      
        1 aatagcgata atagaaaaat caagccggca caaatgacta ccgaaggccc tgctggtgta
       61 tctttccaac tggataacaa taaaccgcca ctgactgcaa taatactgaa gaaaatcgca
      121 atcaacacca ttttttcagg cgttttagca aagcgtcttg ccgtagcaga cggaataatc
      181 agcagtgagg taataattaa agcaccgaca aatttcatac ttaatgcgat agttaatgcg
      241 gttaaaatca tcagcaataa acgtaagcga gccacattta atccttcaat ttgagcaagt
      301 tccgggctga tggtaatcga caacaaccgc ttccaaaaca gcattaaaat caaacaaatt
      361 agggttgctc cgattccgat aaacaccaca tcagaaaaat taattgccag taaatcgccg
      421 aataagtaag acatcaaatc cactcgtaca ttatccaaca aactaatggt aattaccccg
      481 agagaaaggc tagtatgggc gataatcccc aacacggtat ccaccgaata attggttttt
      541 tgctccaacc aaaccagcaa taatgcaaga agaatagtca ttccaattac agcaagatac
      601 ggatcgagtt ctaaaaaaat ccctaatgcc acgccgagca atgcagagtg cgaaagtgtg
      661 tcgccgaaat acgccatttt tcgccacacc acaaaagccc ctagaggtgc ggtaataaag
      721 gctaacaaaa caccggcaag ccaagccgga aataaaatat caaacatatt ggttctcaaa
      781 ggcaagtaaa acaagcggtt attttttcgg aaaaatttgc aaattttttg ctgatttaga
      841 ccgcttgtca tatttattta taacgttgaa tcgggctggc ttgatcctgt gctttcagcc
      901 acgccatttt ttctttaatc tgtttttcca ttccccgctc acttgggaag tagaattcgg
      961 tatctttcag ctcaggcggg aaataattct ccccggcggc ataagcgttt ggctcattgt
     1021 gggcatagcg atactcttcg ccatagccta aagatttcat taattgagtg ggagcattac
     1081 gcaaatgcgc cggcacatcg tagtcttttg cctctttagc gaggcgcttt gcttcattaa
     1141 aagcttgata gaccgcattg ctctttgggg caactgccaa ataaatgatc gcttgggcaa
     1201 tcgctctttc accttcataa gcccccactc gggtaaagca atcccaagcc acaatcgcaa
     1261 tttgcattgc tcgagggtcg gcattaccaa tatcttcaga agcaatcgca agcagccttc
     1321 ttgccacata aagcggatca ccaccggcgg ttaaaattcg ggcataccag tataatgctc
     1381 catcagccga tgagcctcga atggatttat gtaacgcaga aatgagatca tagtaacgat
     1441 caccgccttt atcaaaacga gcttgtcgtt cgcctaacac ttcggtcagc aaacttttat
     1501 tcagcacttt ccctttagct gaatcttgtg ccatatctac cattaattcc aaacagttca
     1561 aggcaaaacg ggcatcacca ttaacataat cagcaagcaa tgccagtaca tcatcttcaa
     1621 gtaccaaagt ttcattgcct aaaccacgtt cggtatcggc tatcgccatt tgcaaaattt
     1681 ttagaatttc gaccgcttgt aacggcttta aaatataaat tctggctcgg gaaagcagtg
     1741 cgttatttag ctcaaaagag ggattttcgg tagtcgcacc aataaaaata atagtgccgt
     1801 cctcaatatg cggtaaaaac gcatcttgct ggcttttgtt aaagcggtga acttcatcca
     1861 caaaaagcaa cgttcgtctg ccggcttggc ggtttaattt ggcttgttca atcacctctc
     1921 ggatctcttt tacaccgctg gtcacagcgg aaatacgttc aacttccgca tcaagatgat
     1981 gggcgataat ttcagctagg gtcgtttttc cggtgccggg cggtccccaa aaaatcatag
     2041 aatgactatg tccggcttca atcgctctgc gtaacggttt gccttcgcca attaaatgag
     2101 attgcccgac atattcagcc aagtttcgag gtcgcatttt ggctgcaagc ggtcgaaaat
     2161 cttcactaaa atcaaaagac aaactactca ttttaacctc ttaacgtaac cgcagttggc
     2221 gacgaatatg acgcattaat aagaaaatcc aagaccaaag cacaccactg attacagcac
     2281 ctaacaattc ctgtgaatta aattcggcac tgtgtaaaat gtattcaatc acgaaaataa
     2341 agaaacggat cagcacaata tatgccatta ctaataggct ttgtagccat agcgataagt
     2401 ttcgcagaat cagatggtat ttcgcaacaa aataaatcgc caccgataac actaaggcgt
     2461 ggatgcctaa aatcgagcca agaatcagat cccagacgat gccggcaata aaagccgtcc
     2521 ccacactcat tttatcaggt aatgccaatg cccaataaat taacaccaaa acgacccaag
     2581 tagggcgtag cccttggaat cctaccggcc aaggcataat ctcaagtaca aaagcaacca
     2641 caaaaatcgc aattatcaca aggatctgaa aaatcgggtt tgttctcatt attccgctcc
     2701 ttcttccgta tgatagtttt gttcattttc cgcttctggt tgtgctgccg gattctcatt
     2761 tatctcatca ttctctattt gttcttcttt tgcctctagc tgcacttcag ttttcttgtt
     2821 agacatctgt tttaaacgtt caaatggatt cacgctatta cgtctttctt caacaacttc
     2881 acgtacttgc gctggcgaca agctttggga gtagtttttc tcttccgccg tcggccaaag
     2941 cagtaataag taacgtaagc gatcaaatga ggctaacgga cgagcaacaa ttctggcaaa
     3001 ttgacttttg gtatcatttt caaccgtttc tacaatcgcc accggatatc cctcagggaa
     3061 acgtccacct aagcctgaag tcaccagcac atcacctttt accacatcaa tagaacgagg
     3121 caagttatca atgaccaatt cattattcac ccctgtacca ctggcaatgc cacgtacatc
     3181 attgcgtagc acttgaacag gaatagcatg agtaatatca gagagcaata aaacacggct
     3241 agatttttcc cctacagaaa taacttgtcc aacaacacct ctttcatcaa taataggttg
     3301 cccgacaaaa gcaccgtcta aacgcccttg attaatcact acttgctgac gataagcatc
     3361 catctcagca gctaatactt cagcaatttt tttatactca tcttgccgaa gcggagaact
     3421 caataacaat cttaaacgct gattttcaac ctttagctga tcaagcaata ataaatcggc
     3481 acttttttct cttagctgct cttttaataa attattctca agctgtaatt tattattatc
     3541 aataaaattg ccacttaccc catctaaaat tgaacggggg gtattagcaa aataatataa
     3601 tccacttacc gcggtttcta atagattacg cgcttgaatc attaaagagc tacgcccatc
     3661 ggaaataatc agcataatag aaaatatcac tgcgataatc aggcgcatcc ctaaagaggg
     3721 agctttagca aaaataggtt tcattagaga aatatatgtg aatttattgg gaatctataa
     3781 cgaaaattga caatagaatc aatccattgc caacctccat ttaaacagct gctaagagaa
     3841 atgtattaat catcaatact aaatacatca ccaccgtgca tatcgatcat ttctaatgct
     3901 ttaccaccac cacgagctac acaagttaat ggatcttctg ctactacgac ttgcacgcca
     3961 gttgaatcag aaagtaaacg atctaaatta tgtaataatg caccacctcc tgttaacacc
     4021 ataccgcgct caaaaatgtc tgctgccaat tcaggtgggc attgctctaa gactgattta
     4081 actgcctcta caataccatt taatggttgt tcaattgctt ctaatacatg tcttgagtta
     4141 agctcgaaag tacgaggagc gccttcagct aaattgtgtc cgtgtacttc cattgtttta
     4201 acatcatcat cggattcaat cttagcacta gcaatttctt gtttaattcg ctctgccgtt
     4261 gcttcaccaa tagcagaacc aaaatgacga cgtacataag caataatagc ttcatctaac
     4321 ttatcaccac caatacgcac agaagttgaa tacacaatgc cgtttaaaga aagcactgcc
     4381 acttcggttg taccgccacc aatgtcaatt accattgacc ccattgcttc gtgaaccggc
     4441 aaacctgcac cgattgcagc cgccattggc tcttcaatta aatagacttc acgcgctcct
     4501 gctccaattg cagattcttt aattgcacgg cgttccactt gtgttgcacc tgctggcacg
     4561 cacactaata cacgagggct tgggcgcata aagttattac tatgtacttg tttaataaag
     4621 tgttgcaaca ttttttctgt tacaaaaaaa tccgcaatga caccatcttt cattggacga
     4681 atcgccatta tgctttttgg tgtacggcct agcattaatt ttgcatgtga gcctaccgcc
     4741 gcaatacttt ttagcgatcc catacgatct tgacgtacag caaccactga gggttcatct
     4801 agcacaatac cttgaccttt aacataaacc agtgtatttg ctgtcccgag atcgatagaa
     4861 agatcgtttg aaaataatcc taaaaatttt ttaaacataa aagaatccgt taaaataaaa
     4921 ttattcaaaa ataagcacat tttatagtgc gaacattgct actaatatag caaaaaagcc
     4981 cgtcattcat caatgaaaaa atttaaactc cactagaaaa tttgcagagc aagttgataa
     5041 gtataaatca gtaaactaaa agcgataaag tacataatta gcccggtaac agcatctaaa
     5101 atttgccaag ttcttcgctt tgcaaaataa ggggaaagta agcttgctcc ataacctatg
     5161 ccaaaaaacc aaataaatga gcaaagtaat gcacctgcta aaaagtatat tttaccgtta
     5221 aaatctaatt tcccaccgat actgcctaca atcacaaccg tatcaatata aacgtgagga
     5281 tttaagaatg taatcgcaaa ggtaatcagt aaagcctttt ttaagctcgt tgcattctct
     5341 ccacttgcca caaggtattc accactttta aatacgctga taaatgaacg gctaccgtag
     5401 cttaatagaa aaatggcccc taaaagcgaa ataatgaaac ttacaacagg caattccgca
     5461 attaacgtgc ctaagcctag cacacctatc gtcattaaaa atacatcacc taaaaaacag
     5521 agcaatgcaa tccaaaatat gtgttgtttt aaaatccctt gctttaataa aaaggcattt
     5581 tgtgcgccaa tagagacaat taagccaaaa cacactataa atccttgaat gaaaatatcc
     5641 attttttctc caaaatcgca taacaagcgg tcaattatac aaaaaatttt gcaaaacttc
     5701 ggtataattt atcgaatact cttttgactg aataggaaat tttttatgac tgatactagc
     5761 cagacttcat cttgcgttgt cattgcgatt gccggcgcat ctgcctcagg taagagcctg
     5821 attgcctcaa ccatctataa agaactcaaa aatgaattgg gaacagataa tatcggtatt
     5881 atttccgaag atgcttatta tagagatcaa tctcatctaa caatggaaga acggattaaa
     5941 accaattacg atcaccctaa ttcaatggat catcatttat tggttgaaca tttacgccaa
     6001 ttaaaacaag gtaatgcgat tgaaattcca gaatatgact ataccgaaca taatcgtaaa
     6061 acgaccacga agcactttga accaaagaaa atcattattt tagaaggtat tttattgctg
     6121 acagatgaga atattcgcaa tgaaattaat gtttctattt tcgtagatgc tcctctggac
     6181 atctgcttta tccgccgctt acaacgtgat atggtggaac gtggacgttc gatggaatca
     6241 gtgattagtc aatatcgcaa aaccgttcgc ccaatgttct tacaatttat cgaaccctct
     6301 aaacaatatg ctgatattat tgtgccaaaa ggtggcaaaa accgtattgc cattgatatt
     6361 cttaaagcac aaattaagca attactaaaa taaaatagga gctaaatatg cgtttatgtg
     6421 atacagatat taaacgttac ttagatgaag gtattattgc cataacgcct cgaccttcag
     6481 acgataaaat ttctggcgcc actgcagatg tgcgtttagg taattcgttt cgtgtatttc
     6541 gtgaacataa aacgccctat attgatttaa gtggccctag agaagaagtt gccgctcagt
     6601 taaataaagt gatgagtgat gaaattatca tcggtgatga tgaagcattt ttccttcatc
     6661 ccggtgacct tgcactcgca acaacccttg aatcggtttc cttaccagcc aatatcgttg
     6721 gatggttaga tggtcgttcg tcacttgcta gattaggctt aatggtacac gttaccgccc
     6781 acagaatcga tccgggttgg gaaggcaaaa ttgtattgga attttttaat gctggaaaat
     6841 tacccctagc tttacgccct aatatggcaa ttggcgcatt aagctttgag attttaagtg
     6901 gtgatgctgc aaaaccctat aatgcgcgaa aagatgcaaa atataagaac cagcaaagtg
     6961 ccatttcaag tcggattaat caagattaat atagtacatg ccttgcaatc atacattaac
     7021 t
//
LOCUS       DANLXF010000126         6989 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.126, whole genome shotgun sequence.
ACCESSION   DANLXF010000126 DANLXF010000000
VERSION     DANLXF010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6989
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.126"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            342..2201
                     /locus_tag="PXD66_001665"
     CDS             342..2201
                     /locus_tag="PXD66_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDL6319949.1"
                     /translation="MSNLKVKVQGFGRFLSNMVMPNIGAFIAWGFITALFIPTGWLPN
                     ETFAKLVGPMITYLLPLLIGYSGGKLVGGERGAVVGAITTMGIIVGSEIPMFLGAMIV
                     GPLGGWAIKTFDKAIDGKVKSGFEMLVNNFSVGIIGMILAMLSFAFIGGIVTQISAAL
                     GAGVGALVEAGLLPVTSIIVEPAKILFLNNAINHGVFTPLGTAQAQEVGKSILFLIEA
                     NPGPGFGILLAYMFFGRGTAKQTAGSASIIHFLGGIHEIYFPYVLMSPRLILAVIAGG
                     ATGVLTNSLLGGGLIAPASPGSIFAILAMTAPGAHFAVVASVILSCAVTFAIASILLK
                     TQKGEASLEDAQAQTQAMKAESKGVVNGNINKIYVACDAGMGSSAMGASMLRKKVQAA
                     GLNIEVQNLAINDLPQDAQLVITHKDLTARATQRVATAQHLSLTNFLDSAFYDSLVAK
                     LANHQPQQAVQNTQNFANPVEEPQAEGLKLQAAQVFLGLQANSKEEAIRFAGEQLVKA
                     GFVQPSYVDAMFAREQMVSTYLGEGLAVPHGTSDAKDSVLKTGIVVCQYPNGVRFTDE
                     EDGVAKLVVGIAAKGNDHIQILSAITNALDDEQAMKTLTSTNDVEKVLALLSK"
     gene            2305..3444
                     /locus_tag="PXD66_001666"
     CDS             2305..3444
                     /locus_tag="PXD66_001666"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005557909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDL6319950.1"
                     /translation="MNALHFGAGNIGRGFIGKLLADARIFVTFADINQTQIDQINQNK
                     QYGVKIVGDDSRVEIVKNIAAINSKDENAVIEQVKNTDLITTAVGPNVLGFIAPLFAK
                     ALVARVESGNTQPLNIIACENMVRGTTFFKGKIFEHLTAEQQAEIEKVVGFVDSAVDR
                     IVPPAEPNPADPLEVTVEEFSEWIVDQTQFKGDIPNIKGMELTDNLMAFVERKLFTLN
                     TGHLICAYLGKQAGVKWIKEAIAIEEIKTQVKATMEESGAVLIKRYGFDPQAHSAYIE
                     KILKRFANPYLNDDVNRVGREPIRKLSENDRLIKPLRGTLEYGLPYQNLVKGVVMALQ
                     YRNEEDPQAVELAEFIANHGVAAAVEKYTGLTDQAVIAQVVELYQ"
     gene            3456..3971
                     /locus_tag="PXD66_001667"
     CDS             3456..3971
                     /locus_tag="PXD66_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MltR family transcriptional regulator"
                     /protein_id="HDL6319951.1"
                     /translation="MMDYIDKLSEETTLRGFLSIANQRFAKAVEQLIERVFRKTDFAL
                     KSVVDSLFEHQGPLADIEVRLKVLLGLGVISPKVFEDISLFLEVKQHLNEEEDEFAFS
                     HPAVVKFAKDLHHIDFLPINELLNMKASAANKDSMLFQMQQMRLEKMVRSSLILAVTE
                     IDEQLNVESPL"
     gene            4111..5976
                     /locus_tag="PXD66_001668"
     CDS             4111..5976
                     /locus_tag="PXD66_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDL6319952.1"
                     /translation="MQSLLSFVPDFLKQELFWVMALLVFAIWQFIQNKLRMDVVALIV
                     MLFFSLTNILSVKEVLAGLSDPNVVLIALLFIVGEGLVRTGVANQVSDWLMRVSGASE
                     TKVLVLLMLCIAGLGSFMSSTGIVAIFIPVVLAICSGMGISPRRLMMPLSVAGLISGM
                     MTLIATPPNLVAHSELVKSGFEGFNFFSFTPIGLIILVMGIGYMLIARRWLDNGEQSN
                     THSQAQKFSMSQLIEEYQLKERAKMVLVEADSDCVGKSIGQLNLRIEQGLNIIAIQRT
                     KRFRKITVSASVNEIFQPKDILLLDTALDDETYQQRCEQLKLRPIELKGEYFSSQYRS
                     VGMAEISVMPEAECIGKTIRELKFRSRYHLSIVGLKRGDEIIQEELLDTPLKFGDILL
                     VMGVWQQIHRMDDDHRDFFLLRDPAESKNAPPALSQAPHALFSVLTMVLLMITGIVPN
                     VMAALIACLMLGKFRCVDMKSAYDSIHMPSIVLIVGMMPFSIALQKTGGVDLTVRLLL
                     DAMQGLDVHFVLIVLFVVTAIISAFISNTATAILVMPIAIAIANQLGYSPAPFVMTVA
                     VSASAAFMTPVSSPVNTMVLAPAGYKFIDFVKVGVPFTVLVMLVSVFVIPMLFPL"
     gene            complement(6266..6517)
                     /locus_tag="PXD66_001669"
     CDS             complement(6266..6517)
                     /locus_tag="PXD66_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6319953.1"
                     /translation="MDKQAILKLFRDNSTAGNSQPSQEPIQVFNNSNVIIQNGDNATV
                     IAGCTVDNNTNLTCADKQRVHDLIHSLEQRNRASRDEGA"
     gene            complement(6529..>6989)
                     /locus_tag="PXD66_001670"
     CDS             complement(6529..>6989)
                     /locus_tag="PXD66_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL6319954.1"
                     /translation="IGFGNRIEQSRKNAKLSRSEVCDALGGIAISTLQAWEADTREPP
                     ISKLMALSQILNVEPCYLLTGERHSQSLNVTREPQPVYATKPQTEEGEVVVIKAKLQE
                     AIETLEEVLEITQREMKPAGKAQMVWAFYELLTQEKSEKEKMVGLLKLVA"
BASE COUNT         1848 a         1447 c         1657 g         2037 t
ORIGIN      
        1 aggctccgct cttctcataa aacttatatt ttctactcat tatgaacctc taaagtgcag
       61 tttatgctga tagtaatata ggtgaacagt tagggttagc aattcatttt attttttcga
      121 tgtagatcgc aaaaatagaa ttatgcttgg cacaagcgag gacgcttacg ccatctttac
      181 tccaaaaatg tgagatagat cacattttca aatctgccca aaaaaattta atgtgatcta
      241 cttcacattt ctgagagaaa catttttgta acttttgaaa gtggtgctaa tttaagcacg
      301 attttttcaa ccctaatttt ctctcctttt ttgaggtttc tatgtctaat ttaaaagtga
      361 aagtccaagg ttttgggcgt ttcttatcga atatggtgat gccgaatatc ggtgccttta
      421 ttgcgtgggg ctttatcacc gccctgttta ttccaaccgg ttggttgccg aacgagactt
      481 ttgctaaact ggtcggtccg atgattactt acttgctgcc actgttaatc ggttacagcg
      541 gtggtaaatt agtcggtggt gagcgtggtg cggttgtagg tgcgattact acaatgggta
      601 ttatcgtggg ttctgaaatc ccgatgttct taggtgcgat gattgtaggt ccattaggcg
      661 gctgggcgat caaaaccttc gataaagcga ttgacggcaa agtaaaaagc ggttttgaga
      721 tgttggtaaa caacttctcg gtcgggatca tcggtatgat tttggcaatg ctttcatttg
      781 ctttcattgg aggtatcgtc acccaaatct ctgcggcatt aggtgcaggt gtgggggcgt
      841 tggttgaagc tggtcttctg ccagtaacct ctatcatcgt tgaaccggcg aaaatcctat
      901 tcttaaacaa tgcgatcaat cacggtgtgt tcacgccatt gggtacagca caagcacaag
      961 aagtgggcaa atctattctg ttcttgattg aagcgaaccc gggtccgggc tttggtatct
     1021 tgcttgccta tatgttcttt ggtcgtggca cagcaaaaca aaccgctggt agtgcgtcaa
     1081 tcattcactt cttaggcggt attcacgaaa tttatttccc gtatgtgtta atgagcccac
     1141 gcttaattct tgcagtgatt gcaggcggtg caaccggcgt tttaaccaac tcattattag
     1201 gtggcggttt aatcgcaccg gcttccccgg gttctatttt tgcgatttta gcaatgaccg
     1261 caccgggtgc tcactttgca gttgttgcct ccgtgattct ttcttgtgcc gtgacttttg
     1321 cgattgcgtc aatcttactc aaaacccaaa aaggtgaagc ctcattggaa gatgcacaag
     1381 cacaaactca agcgatgaaa gcagaaagca aaggcgtagt aaatggtaac atcaacaaaa
     1441 tctatgtagc gtgtgatgcc ggtatgggtt caagtgcgat gggtgcgagt atgttacgca
     1501 aaaaagtgca agcggcaggt ttaaacattg aagtacaaaa cttagcgatc aacgacttac
     1561 cgcaagacgc acaactggtg attacccaca aagacttaac cgctcgtgca acacaacgtg
     1621 tggcaacggc tcaacactta tcgctcacta acttcttaga cagtgcgttc tatgacagct
     1681 tagtggcaaa actggctaac caccagccac aacaagcggt gcaaaataca caaaattttg
     1741 caaacccggt ggaagaacct caagccgaag ggttaaaatt gcaagcagcc caagtctttt
     1801 taggcttaca agctaacagt aaagaagaag cgatccgctt tgccggtgag cagttagtta
     1861 aagcgggctt tgttcaacca agctatgtag atgcaatgtt cgcccgtgag caaatggttt
     1921 caacttactt aggcgaaggc ttagctgtgc cacacggtac atctgatgcc aaagacagcg
     1981 tactgaaaac cggtatcgtg gtctgccaat acccgaacgg cgttcgcttc accgatgaag
     2041 aagacggcgt ggcgaaactg gtggtcggta ttgcagcaaa aggcaacgac catatccaaa
     2101 ttttaagtgc aattaccaac gcactggacg atgaacaagc aatgaaaaca ctgacctcaa
     2161 caaatgacgt ggaaaaagtg ttagcattat tatctaaata atcaccctgt tgtgtagggg
     2221 cgggtcctgt gcccgcccat tatcctacaa aatttagggc gggcacagga cccgccccta
     2281 cgttttatag aggaacaatc atcaatgaac gcactccatt ttggtgccgg taatatcggg
     2341 cgtggcttta tcggcaagtt gcttgctgat gctcgcattt tcgtgacttt tgccgatatt
     2401 aaccaaactc aaatcgacca aatcaaccaa aacaagcaat acggcgtgaa aatcgtgggc
     2461 gatgacagcc gtgtggaaat tgtcaaaaac attgcggcaa ttaactcgaa agacgaaaat
     2521 gccgtgattg agcaagtgaa aaacaccgat ttaattacca ccgcagtcgg cccgaatgtg
     2581 cttggcttta ttgccccact gttcgccaaa gcgttagtgg ctcgtgtgga aagtggcaac
     2641 acccaaccgc tgaacattat cgcctgtgaa aatatggtgc gtggcacaac tttctttaaa
     2701 ggtaaaattt ttgagcattt aactgccgag cagcaagcag aaattgaaaa agtggttggt
     2761 tttgttgata gtgcggtgga cagaattgta ccacctgccg agccgaaccc tgctgatccg
     2821 ttggaagtca cggtggaaga gttcagcgag tggattgtgg atcaaaccca gttcaaaggt
     2881 gacattccaa acatcaaagg aatggaatta accgataacc tgatggcgtt tgtggaacgc
     2941 aaactgttca ccctgaacac cgggcattta atctgtgcct acttgggcaa acaagcgggc
     3001 gtgaaatgga ttaaagaggc gattgcgatt gaggaaatca aaactcaggt gaaagccaca
     3061 atggaagaga gcggtgctgt gctgattaaa cgttacggct tcgacccaca agcccattca
     3121 gcgtatatcg aaaaaatcct caaacgtttt gccaacccgt acttaaacga tgacgtaaac
     3181 cgtgtagggc gtgagccaat ccgcaaactg agcgaaaacg accgcttgat caaaccgttg
     3241 cgtggcacgt tggaatatgg cttaccgtat caaaacttgg tcaaaggcgt ggtgatggca
     3301 ttgcaatatc gcaatgagga agacccacaa gcggtcgaac ttgccgaatt tattgcaaat
     3361 cacggtgtgg cggcagcggt ggaaaaatac accggtttaa ccgaccaagc ggtcattgct
     3421 caagtggtgg aattatatca ataattcttg acactatgat ggattacatc gacaaactca
     3481 gtgaggaaac caccctgcgt ggtttcctta gcattgccaa tcaacgcttt gccaaagcgg
     3541 ttgaacagtt gattgaacga gtgtttcgca aaacggactt tgcgttaaaa tccgtggtgg
     3601 attccttgtt tgaacatcaa ggaccgctgg ctgacattga agtacgttta aaagtgttgc
     3661 ttgggttggg ggtgatctca cctaaagtgt ttgaagatat ctcgcttttc cttgaagtca
     3721 aacagcattt gaatgaggaa gaagacgagt tcgcattttc tcacccggcg gtagtgaaat
     3781 ttgccaaaga tttgcaccat attgattttt tgcctatcaa tgaattgctg aatatgaaag
     3841 cgagtgcagc gaataaagat tcgatgctct ttcaaatgca gcaaatgcgg ttggaaaaaa
     3901 tggtacgttc aagcctgatt ttggcggtaa ctgaaattga cgaacaactg aatgtggaaa
     3961 gcccattgta aaaatttcgt caaaactaac cgcttgtact gagcattcac gccgtacgtg
     4021 attgcttttt cctttctttt tgatcttatc ggcttttttc tttgcttaaa gcggtgtaat
     4081 ctatccctaa ctattttgga ggatatggtt atgcaatcgt tgctctcttt tgtgccggat
     4141 tttcttaagc aagaactgtt ttgggtaatg gcgttattgg tatttgcgat ttggcaattt
     4201 atccaaaaca agttgcgtat ggacgtggtt gccttaattg taatgctgtt ttttagcctg
     4261 actaatattt tatcagtcaa agaagtgttg gcaggcttaa gtgacccaaa cgtggtgctg
     4321 attgcgttgc tctttattgt cggagaaggt ttggtgcgaa ccggcgtggc gaaccaagtc
     4381 agcgattggt tgatgcgggt gtcgggagcg agcgaaacca aagtgctggt gctgctaatg
     4441 ctctgtattg ccgggcttgg atcttttatg agttccaccg gcattgtggc gattttcatt
     4501 ccggtggtgc ttgccatttg ctcgggaatg ggaatttctc ctcgccggtt aatgatgccg
     4561 ttaagtgttg ccggacttat cagcggtatg atgaccttaa ttgccacacc acccaacctt
     4621 gttgctcatt ccgaattagt aaaatcaggt tttgaaggct tcaatttctt cagttttact
     4681 ccgattggat taattatttt agtgatgggc atcggctata tgctgattgc ccgccgttgg
     4741 ttggataatg gtgagcaaag caatactcac agtcaggctc aaaaattttc aatgtcgcag
     4801 ttaattgaag agtatcaact taaagagcga gccaaaatgg tgctggtgga agcagattca
     4861 gactgtgtcg gcaaatctat cgggcaactg aatttacgga ttgagcaagg gctaaacatc
     4921 attgcaattc aacgcactaa gcggtttaga aaaattaccg tcagtgcctc ggtaaacgaa
     4981 attttccagc cgaaagatat tttactgctt gatactgccc tcgatgatga aacttaccaa
     5041 caacgctgtg aacagctcaa acttcgcccg attgagctaa aaggcgaata cttttcctca
     5101 caatatcgct cggtgggtat ggcggaaatt tcggttatgc ctgaagcaga atgtatcggt
     5161 aaaactattc gggaactcaa attccgctca agataccatt taagcattgt cggcttaaaa
     5221 cgtggtgatg aaatcataca agaggaactg ctcgatacgc cactcaaatt cggcgatatt
     5281 ttattggtga tgggcgtatg gcaacaaatt caccgtatgg acgacgatca tcgagatttc
     5341 ttcttgctta gagatccggc agaaagtaaa aacgcccctc cggcgttaag ccaagcccct
     5401 cacgctctgt tctcagtgct gactatggtg ttgctgatga ttaccggcat tgtgccaaac
     5461 gtgatggcag cgttgattgc ctgcttaatg ttaggcaagt tccgctgtgt ggatatgaaa
     5521 agtgcttatg attcgattca tatgccaagc attgtgttga ttgtggggat gatgccgttt
     5581 tctattgcct tacaaaaaac cggtggtgtg gatttaaccg ttaggcttct acttgatgca
     5641 atgcaaggct tagatgtaca ttttgttcta atagtattat ttgtagtaac agcgattatc
     5701 agtgctttta tttccaacac agcgactgca attttggtta tgccgatagc cattgcgatt
     5761 gctaaccaac ttggctactc accggcacct tttgtgatga cggtcgctgt ttcagcctcc
     5821 gccgctttta tgacgccggt ttcttcaccg gtaaatacga tggtattagc accggcaggc
     5881 tataaattta ttgatttcgt taaagttggt gtgccgttta ctgtactggt gatgctggtg
     5941 tcggtgtttg tgattccgat gttatttccg ctctaaacac tcctctaaat tacaagcggt
     6001 catttttggt aatctgacgt tgacggtaaa attgttgagt atgtctggca acatagtgtg
     6061 gggtactgat cttttttgca tagagagaaa gaaaaaaatt tgggcggaaa aataacggcg
     6121 aggctaggaa atacaaggct tcgccgtttt tgtttggggt gagttatgca aaaaacgaaa
     6181 atgagcaaaa atagaaaaaa atgggctatt tttacgttat tttttgcata ggtctgagca
     6241 ggtttaaacg gattttaaaa caattttaag cgccctcatc tcggctggct cggttgcgtt
     6301 gctctaagct gtgaatgagg tcgtgtacac gttgtttatc ggcacaggtt aggttggtgt
     6361 tattatccac cgtgcagccg gcaatcaccg tggcgttatc gccgttttga atgatgacgt
     6421 tgctgttatt aaacacctgt ataggctctt ggcttggctg gctgttgccg gcagtgctat
     6481 tgtctctgaa taatttgaga atggcttgtt tgtccatatt gtcccctgtt atgccaccag
     6541 tttgagtaat cccaccattt tttctttttc gcttttttct tgcgtgagaa gctcgtagaa
     6601 agcccacacc atttgggctt ttccggcagg cttcatttcc cgttgggtaa tttccagcac
     6661 ctcttccaac gtttctattg cctcttgtaa cttagctttg ataacaacta cttccccttc
     6721 ttctgtctgc ggttttgtgg catacacagg ctgtggctct cttgtgacat taaggctttg
     6781 cgagtgtcgc tcacctgtta ataggtagca aggctcaacg ttaagtattt gtgacaaagc
     6841 cataagtttt gatataggag gttctcttgt atcagcctcc cacgcttgta atgtagatat
     6901 tgcaatacct cctagggcat cacaaacctc agatctactt aattttgcat tttttctgct
     6961 ctgttctatt cggttaccga aaccaataa
//
LOCUS       DANLXF010000127         6978 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.127, whole genome shotgun sequence.
ACCESSION   DANLXF010000127 DANLXF010000000
VERSION     DANLXF010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6978
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.127"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(95..964)
                     /locus_tag="PXD66_001671"
     CDS             complement(95..964)
                     /locus_tag="PXD66_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL6319955.1"
                     /translation="MFKKYFGEIILFLVTFIAAAGWFFSKHALVGLPPIGFMGGRFIL
                     ASLILLPFSYRELYRLDKVQVKYSVITGLTYGIYTIFWVLGLAFSTVFGEGAFLVSLA
                     MLIAPLLSWLIFKHTPPKIFWLALPIALSGLYFLSAEKGIFHFSLGSTIFLVSSVFGA
                     LYFVLNNQYAKQVSLFPLITIQLAIVGAVCSLYSYFMESWHFPILTEVWLWFAASVLI
                     ATNLRFLMQTLGQKYCHIGTSALIMLLEPVWTLLLSVMLLGEQLSATKWLGCSLILIA
                     LIIYRLPILRLKK"
     gene            complement(1015..2199)
                     /gene="uxuA"
                     /locus_tag="PXD66_001672"
     CDS             complement(1015..2199)
                     /gene="uxuA"
                     /locus_tag="PXD66_001672"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012341261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL6319956.1"
                     /translation="MEQAWRWYGPNDPVSLDDVRQAGATGIVTALHHIPNGQVWSVEE
                     INKRKALLAEKGLVWSVVESVPVHEEIKTQTGNYQQWIDNYKQTLRNLASCGIDTVCY
                     NFMPVLDWTRTDLEYEMPDGSKALRFDQVAFAAFELYILKRPGAEATYSSAEQAEAKA
                     YFEQMSDADIAKLTKNIIAGLPGSEEGYTLEEFQAQLDRYQGLSAEKFRTHLAYFLNE
                     IVPVAQEVGIRMAIHPDDPPRPILGLPRIVSTIEDMQWFVETQPLAANGFTFCTGSYG
                     VLAENDLVKMAEQFADRIYFAHLRATQREENPQSFHEADHLAGDVDMFGVVKALLTEE
                     YRRKANGDHRLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLRGLAEFRGLELALKKVYF
                     ND"
     gene            complement(2229..2990)
                     /locus_tag="PXD66_001673"
     CDS             complement(2229..2990)
                     /locus_tag="PXD66_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HDL6319957.1"
                     /translation="MQNSSRTYTKIGKILKEEIAQGKYRVGDKLPTERDISERFEVSR
                     TIVREAIVMLEVEKLIEVKKGSGVYVIRVPELFPEDFPEEKDNELRDVGPFELLQARQ
                     LLESSIAEFAAQQATKQDIQVLRNILESEKKLLLEKMDDYSADKEFHLALAHMTGNDV
                     LIQLQEQLWRYRFNSAMWAQLHSRILQHDHHHLWIEDHERILNAIQRKNPALARKAMW
                     QHLENVKTKLLELSDVEDPKFDGYLFNVNPVVVGI"
     gene            3154..4179
                     /locus_tag="PXD66_001674"
     CDS             3154..4179
                     /locus_tag="PXD66_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDL6319958.1"
                     /translation="MEKPLKSVCLVEPNKVGIQTVSYPVKGENDVLIKVEGMGICGSD
                     IGAFRGTNPLVTYPRILGHEIVGTVIESGKGMPNNINVGDRVIIEPYIYCGHCYPCSI
                     GRTNCCESLKVLGVHIDGAMQEVVRHPAHMIIKTPDMPIHELALAEPLTISLHAIHRT
                     KVKAGEHVAIIGAGAIGLMAALVAKAYGATPILIDILDKRLDYAKSIGIPHTINPTKE
                     NDLEAIKSITNGRMAEVVIEASGANVAVQNTLKYTSFAGRIALTGWPKTETPLPTNLI
                     TFKELNIYGARTSKGAFEEALELLASRRIEPKDIISKVITFDEIPHYIEELSANPDDY
                     LKIIAVF"
     gene            4208..5191
                     /locus_tag="PXD66_001675"
     CDS             4208..5191
                     /locus_tag="PXD66_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDL6319959.1"
                     /translation="MKLKTFTKALLISSLMFTGSAFAKTIIKIGHYNSDVHPSNIALQ
                     EYFKKTIEEKTEGRYEIRIFPNNQLGGEDQIVNGLRNGTIEGGITGLLLQNVDPIFGV
                     WEWPYLFKDNAEAKKVLESPIAQEIGEKMEKYGIKLLAYGMNGFRVISSNKKVENFDG
                     FKGLRLRVPLNSLFVDWAKAMHINPQSMPLSEVFTALEQKVIDGQENPYMLIKDSGLY
                     EVQKYIIESNHIFSPGLLQLSLKTWNKMSKEDQTIFLEAAKLYQEKEWELAMKMEQDV
                     KDFFHKNGKEVIVPSEQFKADMLKASETLYQNFYQKYDWAKDVISKIHAAK"
     gene            5252..5731
                     /locus_tag="PXD66_001676"
     CDS             5252..5731
                     /locus_tag="PXD66_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDL6319960.1"
                     /translation="MQISTALDRTLKLLAIIALSTMIILVFFNAVLRYFFDSGIAWSE
                     EFARICFVYMIFLGIILVAKERGHLTVDIVVSALPAKVQMYLLFFADILVLVALSFIT
                     YGAYQLMLLTYTQKMPATEISSAFLYFAAVISSISYFAIVFTNLWKHIRLAFIKEEK"
     gene            5734..>6978
                     /locus_tag="PXD66_001677"
     CDS             5734..>6978
                     /locus_tag="PXD66_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HDL6319961.1"
                     /translation="MLWLFLGSLFLFLFLGVPVALSMLLSASFMLWKMDMFDAQLITE
                     NFVMGTNNFPLMAIPFFMLTGEIMKHGGISERIINFAMSMVGHIKGGLGYVVIISGLI
                     FAGLSGSAVADTAALGAILIPMMVARKYDVARSTGLICAAGIISVVIPPSIPMIVYGI
                     TAGASITKLFMGGMVPGFLMVLGLWLVWKFLYRNEEQTVEPKKTGKERWAAFKSAFLP
                     LLLPIIIIVGLRGGIFTPTEAGVVAAIYAAIISIILKGLTLDKIQDVFLGTIKTTAMV
                     MFVAAAAMISAFAVTVAQIPMELVEFIKGITDSPTVLMFVIMIFLLLVGCVMDLIPAV
                     LIFVPVLLPLLRAYNIDIAYFGIMMVINLSIGLITPPVGTVLYVGSGIAKIGIARLTK
                     GIYPFLLVYAAILFLLILFPEII"
BASE COUNT         2173 a         1384 c         1305 g         2116 t
ORIGIN      
        1 tttttgcaag attttttgca aaaatgaccg cttgttactt actctaaaat gcctaactgc
       61 ttccaatact atggcatatt tgcttttgtt ttacctattt tttgagtcgt agaataggga
      121 gtcgataaat aatcaatgct atcaaaatca agctgcaacc tagccatttt gtcgcactaa
      181 gttgttcccc caataacatc acgctcaata acaatgtcca aaccggctca agtaacataa
      241 tgagtgcaga agtaccaata tggcaatatt tttgccccag tgtttgcatt aaaaaacgta
      301 aatttgtcgc aatcaataca cttgcggcaa accagagcca cacttcggtg agaatcggga
      361 aatgccaact ttccataaaa taggaataca agctacagac cgcaccgact atcgccaatt
      421 ggatagtaat taatggaaac aatgagactt gtttggcgta ttgattattt aacacaaaat
      481 ataacgcccc aaacactgat gacaccagaa aaatcgtact gcctaaggaa aaatgaaaga
      541 tgcctttctc cgcagaaaga aagtaaagcc cacttaatgc aatgggtaag gctaaccaaa
      601 aaatcttcgg tggcgtatgt ttgaatatca accaagagag caaaggagca atcagcatcg
      661 caaggctcac caaaaaagcc ccttcaccaa acactgtact aaaagccagc cccaataccc
      721 aaaaaatagt ataaattccg taagtaagac cggtaatcac cgaatattta acttgaacct
      781 tatctaaacg atacagctct cggtaagaaa aaggcagaag aatcaaagag gctaatataa
      841 aacgccctcc cataaagcca ataggcggta atccaactaa agcgtgttta gagaaaaacc
      901 accccgctgc agcaataaag gtaacgagaa aaaggattat ttcgccgaaa tattttttga
      961 acatatcaaa ctataaaatg ccctctacag tagagggcat tataataaat aaggctaatc
     1021 gttaaaatag actttcttaa gtgccaactc caatccacgg aactccgcta atcctcttaa
     1081 acgaccaatc gcagaatagc ctgggttggt ttttttcttc agatcatcga gcatttgatg
     1141 gccgtgatcc ggacgcattg gaattaatcg gtgatcgcca tttgccttgc ggcgatactc
     1201 ttcggtaagt aatgctttca ccacgccaaa catatcgaca tcaccggcaa gatgatccgc
     1261 ttcgtggaaa ctttgtggat tttcttcacg ttgggtagct cgtaagtgag caaaataaat
     1321 gcgatctgca aactgctctg ccattttgac caaatcattc tcggctaata cgccgtatga
     1381 accggtacag aacgtgaagc cattggctgc aagcggttga gtttccacaa accattgcat
     1441 atcttctatg gttgaaacaa tgcgtggcaa gcctaaaatc gggcgaggcg gatcgtcagg
     1501 gtgaatcgcc atacgaatgc ccacctcttg tgcaaccggc acaatctcat ttaagaaata
     1561 ggctaaatgg gtacggaatt tttcagccga gagcccttga tagcggtcaa gctgtgcttg
     1621 gaattcttcg agcgtatagc cttcctcaga ccccggcaaa ccggcaatga tatttttggt
     1681 taattttgca atatcagcgt cactcatctg ctcaaaatag gctttggctt cggcttgttc
     1741 tgctgatgag taagtggctt ccgctcccgg gcgttttaag atatataact caaacgccgc
     1801 aaacgccact tggtcaaaac gcaatgcttt tgagccatca ggcatttcat actccagatc
     1861 ggtacgagtc caatctagca ccggcataaa gttatagcac accgtatcaa tcccacagct
     1921 tgccaaatta cgcaaggttt gcttatagtt atcaatccat tgctgatagt tgcctgtttg
     1981 agttttgatt tcttcgtgaa caggcacact ttctaccacc gaccaaacca gccctttttc
     2041 agctaataat gccttacgtt tattaatttc ctcaaccgac cacacctgcc cgttgggaat
     2101 atggtgcaac gctgtcacaa tccccgttgc ccctgcttgt cttacatcat ctaaagatac
     2161 cggatcgttt ggtccatacc aacgccatgc ttgttccata gtaaactcct cattgtgtag
     2221 tttaggtttt agatacctac tacaacgggg tttacattga atagatatcc atcaaattta
     2281 ggatcctcta catcagacaa ttcaagcaat ttagttttaa cattttctag atgttgccac
     2341 attgctttac gagctaatgc aggatttttt ctctgtattg catttaaaat tcgctcatga
     2401 tcctctatcc acaagtggtg atgatcatgc tgtaaaattc tggagtgtag ctgagcccac
     2461 atcgcactat taaagcggta tcgccaaagc tgttcttgta attgaatcag aacatcgtta
     2521 cccgtcatat gagctaatgc aagatgaaat tccttatctg cactgtagtc atccatcttc
     2581 tctaacaaaa gtttcttctc actttctaat atgttacgaa gtacttgtat atcttgtttg
     2641 gtagcctgct gtgctgcaaa ctcagcaata ctgctttcta ataattgtcg tgcttgaagt
     2701 agttcaaatg gcccaacatc ccttagctca ttatctttct cttctggaaa atcttctgga
     2761 aaaagctccg ggactctaat gacatacacg cccgatcctt tttttacctc aataagcttt
     2821 tctacttcta gcatcactat cgcctccctt acgatagttc tactcacttc aaatctttca
     2881 gaaatatctc tctcagtagg taacttatct cctactctgt attttccttg agcaatttcc
     2941 tctttgagaa tttttcctat tttagtgtaa gttctggaac tattttgcat aaatcctcac
     3001 gaaattggaa ttccaaaatt tcatttttgt gatcaggttc acaaaatcac aaaaattgga
     3061 atgccaaact tgttttttgg tgttagtatc tgtgcataaa ataccattga cactacaaaa
     3121 tgtcgattaa ctttatcaaa aaaggacaaa atcatggaaa aaccactcaa atcagtatgt
     3181 cttgtagaac ctaataaagt tggtatacaa accgtttctt atcctgttaa aggtgaaaat
     3241 gatgtgctaa tcaaagtaga aggtatgggg atatgtggct ctgatattgg agcatttaga
     3301 ggcactaatc cacttgttac ttatcctaga attttagggc atgaaatcgt tggcacagta
     3361 attgagtctg gtaaaggtat gccaaataat attaatgtag gagatcgtgt catcattgaa
     3421 ccttacatct attgtggaca ttgctatccc tgctccattg gtagaacaaa ttgttgcgaa
     3481 tctctaaaag tattgggggt acatattgat ggggcaatgc aagaggttgt tagacatcca
     3541 gcccatatga taatcaaaac acctgatatg ccaatacacg aattagcgtt agcagaaccc
     3601 ttaactattt ccttacacgc aattcataga acaaaagtca aagctggtga acatgttgct
     3661 atcattggag caggggcaat tgggctaatg gctgcattgg ttgccaaagc ttatggagcg
     3721 acgccgattt taattgacat tttagataag cgattggatt acgcaaaatc tattggtatt
     3781 ccacacacaa ttaatccaac aaaagaaaac gatcttgaag ctattaaatc aattaccaat
     3841 ggaagaatgg cagaggtggt tattgaagcg tcaggagcaa atgtcgcagt acaaaatacg
     3901 cttaaataca cctcttttgc tggacgtatt gctttaaccg gatggccgaa aacagaaaca
     3961 ccattaccaa ctaatttaat caccttcaaa gaacttaata tttatggggc aagaacaagc
     4021 aagggggcat ttgaagaagc attagagctt ctggcatcaa gaagaattga accaaaagat
     4081 atcattagta aggtaattac atttgatgaa atacctcact atatcgaaga actctcagct
     4141 aatcctgatg attacttaaa aatcatcgct gtattttaaa tactaactca cctacgaggt
     4201 aataactatg aaactcaaaa ctttcactaa agctctatta atttctagcc tgatgttcac
     4261 cggttcggca tttgcgaaga ctatcattaa gattggtcac tataattcag atgttcatcc
     4321 atcaaatatc gcattgcagg aatacttcaa aaaaaccatt gaagaaaaaa cagaaggacg
     4381 ctacgaaatc cgtattttcc caaataacca gttgggagga gaagatcaaa ttgtaaatgg
     4441 tttgcgtaat ggcacgattg aaggtggcat tacaggttta ttactacaaa atgttgatcc
     4501 aatttttggc gtatgggagt ggccatatct atttaaagat aatgccgaag ctaagaaagt
     4561 tctagaatct cctattgctc aagaaatcgg ggaaaaaatg gagaagtacg gcattaaatt
     4621 actagcgtat ggtatgaatg gcttccgtgt tatctcttcc aataaaaaag ttgagaattt
     4681 tgatggattt aaaggtttgc gattaagggt tccactaaat tcgttgtttg tggactgggc
     4741 aaaagcaatg catattaacc ctcaaagtat gcctttaagt gaagtattta ccgcactaga
     4801 acaaaaagtc attgatgggc aagaaaatcc atatatgtta attaaagatt ctggtttata
     4861 cgaggtgcaa aaatatatta ttgaatctaa tcatattttc tcacctggct tactacaact
     4921 tagtttgaaa acttggaata aaatgtctaa agaagatcag acaatcttct tagaggcagc
     4981 aaaactctac caagaaaaag agtgggaatt agcgatgaag atggaacaag atgtaaaaga
     5041 tttcttccac aaaaatggca aagaagtcat tgttccgagt gaacaattta aagctgatat
     5101 gttaaaagca tccgaaacgc tttatcaaaa tttctatcag aaatatgatt gggctaaaga
     5161 tgttatttca aaaatccacg cagccaaata agcaataagc gaggcgaccg gataaaagag
     5221 gtcgcccatc taaatgaaga aggagaaatt tatgcaaatt tctacagcat tagatagaac
     5281 tctcaaactc cttgcgataa ttgctctatc tacaatgatt atcctagtct tttttaatgc
     5341 ggttttacga tatttttttg actccggaat agcttggtct gaggaattcg ccagaatctg
     5401 ctttgtctat atgatctttc taggaataat cctcgttgca aaggaaaggg gacacctcac
     5461 cgttgatatt gttgtttctg cactaccggc aaaagtacaa atgtacttac tgttctttgc
     5521 tgatatttta gtcttagttg cactgtcatt tattacttac ggtgcatacc agttaatgtt
     5581 attgacatac acacaaaaaa tgccagcaac tgaaatttca tctgcgtttc tatattttgc
     5641 agcagttatt tcatctatct cctatttcgc tattgtattc acaaatttat ggaaacatat
     5701 tcggctagct ttcattaaag aggagaaata aatatgttat ggctattttt aggaagtcta
     5761 ttcctctttc tctttttagg agtgcccgtt gctctctcta tgctacttag tgccagtttt
     5821 atgctatgga aaatggatat gtttgatgcc caattaatca cggagaattt cgtaatgggt
     5881 acaaataatt ttccgctgat ggctatcccg ttttttatgc tgaccggtga aattatgaaa
     5941 cacggcggta tttccgaacg tattatcaat tttgcaatga gcatggtcgg ccatattaaa
     6001 ggcggattgg gctatgtagt gattatttca ggcttaattt tcgcaggttt atccggctcc
     6061 gctgttgctg ataccgcggc tctcggtgca attcttattc cgatgatggt tgcaagaaaa
     6121 tatgatgttg cacgttcaac aggcttaatc tgtgcagcag gtattatttc tgtagttatc
     6181 ccaccaagca ttccaatgat tgtctatggt attacagccg gtgcatcaat taccaaatta
     6241 tttatgggag gaatggttcc aggcttttta atggttttag gtttatggtt agtttggaaa
     6301 ttcttatacc gaaacgaaga acaaaccgtt gaaccgaaaa aaacaggtaa agaacgttgg
     6361 gctgcattta aatcagcatt cttaccattg ctacttccaa ttattattat cgtaggttta
     6421 agaggcggca tctttacacc aacagaagcg ggagttgtag cagcaattta tgcagcaata
     6481 atcagcatta tcttaaaagg gttaacctta gataaaatcc aagatgtttt cttaggcact
     6541 atcaaaacaa ctgcaatggt tatgtttgtt gcagcggcgg caatgatatc tgcatttgca
     6601 gttactgttg ctcaaatacc tatggagtta gttgagttta tcaaaggtat taccgatagc
     6661 ccgactgtac taatgttcgt cattatgatt ttcttattat tagtcggctg tgtaatggac
     6721 ttaatcccag cagtattaat ttttgtacca gtactattac cattactaag agcgtataac
     6781 atcgatattg cttactttgg tattatgatg gtaattaacc tatctatcgg tttaattacg
     6841 cctcctgttg gaacggtatt atatgttggt tctggtatag caaaaattgg tattgctaga
     6901 ttaaccaaag gtatctatcc attcttgcta gtctatgctg caatactgtt cttattaata
     6961 cttttccctg aaattatt
//
LOCUS       DANLXF010000128         6955 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.128, whole genome shotgun sequence.
ACCESSION   DANLXF010000128 DANLXF010000000
VERSION     DANLXF010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6955
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.128"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..286
                     /locus_tag="PXD66_001678"
     CDS             <1..286
                     /locus_tag="PXD66_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HDL6319962.1"
                     /translation="SSITQDPTEFVIYFKGLGTTIEFNINDILKKIFAKHQLSITQQH
                     KNEVLEKIKGDLLKMDLGNSVLGLKHIELRFEDNIGYIVESISTQYIMLK"
     gene            complement(329..3358)
                     /locus_tag="PXD66_001679"
     CDS             complement(329..3358)
                     /locus_tag="PXD66_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4981 domain-containing protein"
                     /protein_id="HDL6319963.1"
                     /translation="MSLPNYYQDPSVLHLNTTLHHAYFIPFAKGQTPNQLEREQSELF
                     TLLNGDWKFNFYPSPHDLPEDFLNTKFTHQIPVPSNWQTQGFDAHQYTNINYPIPFDP
                     PYVPHQNPCGVYQREITFNPKPNKRYLLNFEGVDSCLFVYVNGEFVGYGQISHSTNEF
                     DITKQLKQGKNSLVVVVLKWCDGSYLEDQDKFRMSGIFRDVYILEREQNYLQDFFIRY
                     QLNADLSKAELKVETQFSTYTEAVHFQLFDPENRLVCEQTSGDFCQEICNLCLWNAED
                     PTLYTLCLSYQNEIIEQKIGFRQIRVENGVMLFNNAPIKFKGVNRHDSDPKTGYSISR
                     EQALVDLRLMKAHNINAIRTAHYPNAPWFSELCDQLGFYLIAESDLESHGSSAVYIES
                     PENSIFLNVPNPNKQEEIRQQAVDNFCYFARDPLFKQAILDRNIANVERDKNRTSVII
                     WSLGNESGFGENFEVAARWVKQRDPSRLTHYEGSIYQHSAHQNDLSNLDLYSEMYPST
                     ERLEEYFSDSANHKPFILCEYSHAMGNSSGDMEDYWQTFQRYSGACGGFVWEWCDHAP
                     LLPNSELSAEQKTEKYGYGGDFGETLHDGNFCMDGLVSQQRVPHSNLLEVKNVNRPVR
                     AELKAGKIWLKNQLDFSDLADYLTVHYCFSENGETTLQGQCLVNCQPNQTACLPLTLP
                     EPNGKLQLLTLDYSLNESTALLEKGHHLGFDQITLHQAMVTPALNPRPQAVIFVQDFA
                     NEWRIQQGDYRYTFDKLKGIFSRIEKNGEAVIEQPLDFNIWRAPTDNDRLIREFWKKA
                     GYDNCYTRAYHTELMQDKNGVNITAECGIVATAQSRILTLKVNYHLAPNGEMTIQVQA
                     TRPAHLPFLPRFGLRFFLNKRNNQVSYFGYGEQESYIDKYHLAKLGAYQTSARENHTD
                     YLKPQENGSHFGTHYVQANGITVTSPQPFSFNLSPYTQEELTTKSHSYDLVESPSTVL
                     CVDYKMSGIGSNSCGPNLKPQYRLNETNFEWLLKLSF"
     gene            3536..4393
                     /gene="menB"
                     /locus_tag="PXD66_001680"
     CDS             3536..4393
                     /gene="menB"
                     /locus_tag="PXD66_001680"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005723289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HDL6319964.1"
                     /translation="MLYPSEEFLYAPVEWVDHSEGYTDIRYHKSKDGIAKITINRPEV
                     RNAFRPQTVKEMIEAFSDARFDEKIGVIVLTGEGEYAFCAGGDQKVRGDYGGYKDESG
                     VHHLNVLDFQRDIRTCPKPVVAMVAGYAIGGGHVLHMLCDLTIAADNAKFGQTGPKVG
                     SFDGGWGASYMARLVGQKKAREIWFLCRQYNAQEALDMGLINTVVPYADLEKETVRWC
                     REMLQNSPIALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNEKREP
                     DFSKFRRNP"
     gene            4476..6878
                     /gene="mrcB"
                     /locus_tag="PXD66_001681"
     CDS             4476..6878
                     /gene="mrcB"
                     /locus_tag="PXD66_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1B"
                     /protein_id="HDL6319965.1"
                     /translation="MSDVELNEQSEQEPEQKPKKRNYFLSTLFKIGVIGACSMAFYGI
                     YLDGKIRSKMDGQIWELPAEVYARIESIKIEDNLTLEQTKQALLDNGYRQVSQVATPG
                     DFKIEENQLVLLRRAFPFPETPESQRVLRLTFKQDKLTYIEDLVQGKLIEEFRLDPKL
                     LALLHSDSDEERQALRLQQYPRLLIESLILTEDRRFYQHDGISPIGIARALITNYQAG
                     RTVQGGSTLTQQLVKNLFLSNERSLVRKVNEALMSVILDFRYDKNRILETYLNEIYLG
                     QNGSYQIHGFALASQFYFGRPIQEISLDQIALLVGMVKGPSLYNPWRNPQGAVERRNV
                     VLRLLLDHQVINQELYALLSSRPLNIREKGTIYREQPAFMQALTIDLKNELGEIKSAQ
                     LSGTKIFTTLDRKQQRSAEQAVITGLEDLESKNKKVSDLQSAIVVAEYRTGKVRAIVG
                     DRLTQFAGFNRAIQSKRQIGSLVKPSVYAIALAQPELFRLNTPIQNKQISIKVGSTYW
                     TPRNYDRSFSGSVMLMDALVRSLNIPTVNIGMKVGLKKVIAKQKEMGWDNAEIPPYPS
                     TLLGSYSISPYDVTRSYQVIANEGLKTPLTTIEAIISHNGEMFYQRDSGKVSEQVLPP
                     EAAVQTMYAMQQVVERGTARSLQNDFAKLKLAGKTGTTNNARDTWFVGIDGEHVTTVW
                     LGKDNNGDTNLTGSSGALYVYKQYLERALPQAYRLPKLNTMQWVGINSYGSWNCDSGR
                     QIPVWRDKGQRYCTSAGNVVATEKPSVWDALSLKKENIEAKAEEAEGKQPIPTEEVAP
                     IE"
BASE COUNT         2022 a         1421 c         1591 g         1921 t
ORIGIN      
        1 ctcttcaata acacaagatc ctactgaatt tgttatctac tttaaaggac ttgggactac
       61 tattgaattt aacattaacg atattttgaa aaaaatcttt gctaaacatc agttatctat
      121 tacccagcaa cacaaaaatg aggttttaga aaagattaaa ggcgatttac tcaaaatgga
      181 tttaggaaat tcagtacttg ggcttaagca tattgagtta agatttgaag acaatatcgg
      241 ctatatagta gaaagtatta gtacacaata tattatgctg aaataaaagg agatgctcgt
      301 tcaattaaat ttgaacgagc atttttcgtt aaaaactcaa tttaagcagc cactcaaaat
      361 tagtctcatt taaccgatat tgcggtttca aattcgggcc gcaagagttt gagccaatgc
      421 cgctcatttt gtaatcgaca cacagcacgg tggaagggct ttctaccaaa tcataactgt
      481 ggcttttagt ggttagctct tcctgtgtgt aaggcgaaag attaaaacta aacggctgtg
      541 gagaagtaac agttatccca tttgcttgca cataatgcgt accgaaatgg ctgccgtttt
      601 cctgcggttt gagataatcg gtatgatttt ctcgggcgct ggtttgataa gctccgagtt
      661 tcgccaaatg gtatttgtcg atataactct cctgctcgcc atagccaaaa tagctcactt
      721 ggttattccg tttattcaag aaaaaccgca agccaaagcg aggtaaaaac ggcaaatgag
      781 cggggcgggt tgcctgaacc tgaatcgtca tttcgccatt cggggcaaga tgataattca
      841 ctttcagggt taaaatccga ctttgagcgg ttgccacaat gccacattcg gcagtaatgt
      901 ttacgccgtt tttatcttgc atcagttcgg tatgatacgc cctggtgtaa cagttatcgt
      961 agccggcttt tttccaaaac tcacgaatta agcggtcgtt atcggttggg gctcgccaaa
     1021 tattgaaatc caacggttgc tcaattactg cctcgccgtt tttctcaatc cggctgaaaa
     1081 tgcctttgag tttatcgaaa gtgtagcgat agtcgccctg ttgaatccgc cattcatttg
     1141 caaaatcttg cacaaaaatg accgcttgcg ggcgtggatt aagtgctgga gtgaccatcg
     1201 cttgatgcag cgtgatttgg tcaaagccta agtgatgccc tttttccaac agagccgtgc
     1261 tttcatttaa agaataatca agggttaaaa gttgcaattt gccgtttggt tcgggcagcg
     1321 ttagcggcaa acaagcggtc tgatttggct gacaatttac caaacattgt ccctgcaggg
     1381 tggtttcgcc gttttcgctg aaacagtaat gcacggtcag ataatcagct aagtcgctga
     1441 aatccaactg atttttcagc caaattttgc ccgcttttaa ctcagctcgc accgggcgat
     1501 tcacattttt cacttctaac aaattagagt gtggcacacg ctgttgggaa accaagccgt
     1561 ccatacagaa attgccatcg tgcaaggttt cgccgaaatc accgccatag ccgtattttt
     1621 cggtcttctg ttcggcactt agctcagagt tggggagcag aggagcatgg tcgcaccatt
     1681 cccacacaaa gccaccgcac gcaccgctgt agcgttggaa ggtttgccaa taatcttcca
     1741 tatcaccact cgaattgccc atcgcgtgag aatattcaca caaaataaaa ggtttgtggt
     1801 tggcagagtc ggaaaaatat tcctctaacc gctcggtgct tgggtacatc tcgctgtata
     1861 aatcgaggtt tgaaagatcg ttttgatgtg ccgaatgttg gtaaatcgaa ccttcgtagt
     1921 gagttaaacg gcttggatcc cgttgtttta cccaacgggc tgcgacttca aaattttcac
     1981 caaagccgga ttcgttacca agtgaccaaa taatgaccga agtgcggttt ttgtctcgct
     2041 ccacattggc aatgttgcga tctaaaatcg cctgtttgaa cagcggatct cttgcaaaat
     2101 aacagaaatt atcgaccgct tgttggcgaa tttcttcctg tttgttcggg tttggcacat
     2161 tcaggaaaat gctgttttcc ggactttcaa tatacactgc actggagccg tgagattcaa
     2221 gatcactttc ggcaatcaga taaaaaccga gctgatcgca caattcacta aaccacggag
     2281 cgttcgggta atgggcggtg cgaatagcgt tgatgttatg ggctttcatt aagcgtaaat
     2341 ccaccaacgc ttgctcacgg ctaatgctgt agccggtttt cgggtcggaa tcgtggcggt
     2401 ttaccccttt aaatttaatc ggggcgttgt tgaatagcat cacgccattt tccacacgaa
     2461 tctgacgaaa gccgattttt tgctcaataa tttcgttttg gtagctcaag catagcgtgt
     2521 agagtgtcgg atcttccgca ttccacaaac acaaattgca aatttcttgg caaaaatcac
     2581 cgcttgtttg ctcgcagact aagcggtttt cagggtcgaa aagttgaaaa tgcacagctt
     2641 cggtgtaggt ggaaaattgt gtttccacct tgagttctgc ctttgaaaga tcagcgttta
     2701 actggtagcg gataaagaaa tcttgcaggt agttttgctc acgttccaaa atataaacat
     2761 cacggaaaat gcctgacatt cggaatttgt cttgatcttc caaataactg ccgtcacacc
     2821 attttagcac caccacgacg aggctgtttt tgccctgttt aagctgtttg gtgatgtcaa
     2881 attcattggt gctgtggctg atttgcccgt agccgacaaa ttcgccgttt acataaacaa
     2941 acaggcacga atctacgccc tcaaaattta gcaaatagcg tttgttaggc ttggggttaa
     3001 acgtgatttc acgctgatac accccgcaag ggttttggtg tggcacataa ggcggatcaa
     3061 acggaatcgg gtagttgatg ttagtgtatt gatgggcatc aaagccttga gtttgccagt
     3121 tagacggcac ggggatttgg tgggtaaatt tggtgttgag gaaatcctcc ggcagatcgt
     3181 gcggacttgg atagaaatta aacttccaat cgccatttag caaagtaaat aactcagatt
     3241 gttcccgttc caactgattc ggcgtttgcc ctttagcaaa aggaataaaa taagcgtggt
     3301 gtagcgtggt attaaggtgc aacacactcg ggtcttggta gtaattcggt aacgacataa
     3361 aagccctatg atcaaaaaga tataaatacg aatacacttt aattctctta taaaataggg
     3421 caatcaacta tgtgaaaaat tgtgaggcgg atcacaaaat aatttcctca cctttttcct
     3481 tcgattccgt tataatcaca agaatttttt atgtcatttt tcaaggaatt taccaatgtt
     3541 atatccaagc gaagaatttt tatatgcacc ggttgagtgg gttgatcaca gcgaaggtta
     3601 caccgatatt cgttaccata aatcaaaaga cggtatcgct aaaatcacca ttaatcgtcc
     3661 ggaagtgcgt aatgctttcc gtccgcaaac ggtaaaagag atgattgaag ccttttctga
     3721 tgcccgtttt gatgaaaaaa tcggcgtcat cgtattaacc ggtgaaggcg aatatgcgtt
     3781 ctgtgccggc ggcgaccaaa aagtgcgtgg cgattacggc ggctacaaag atgaaagcgg
     3841 tgtgcatcac ttaaatgtgt tggatttcca acgtgatatt cgtacctgcc cgaaaccggt
     3901 ggtggcaatg gttgccggct atgcgattgg tggtggacac gttttacata tgttatgtga
     3961 tttaaccatt gccgctgaca atgcaaaatt cggtcaaacc ggcccgaaag tggggtcatt
     4021 tgacggcggt tggggtgcaa gctatatggc acgtttagtc ggtcagaaaa aagcgcgtga
     4081 aatttggttc ttatgccgtc aatataatgc acaagaagcg ttagatatgg gcttaatcaa
     4141 taccgttgtg ccttatgctg atttagaaaa agagactgtg cgttggtgtc gcgaaatgct
     4201 acaaaacagc ccgattgcct tacgttgctt aaaagcagca ttgaatgcag attgtgacgg
     4261 tcaggctggc ctgcaagagc ttgctggtaa tgcaacaatg ttgttctata tgacagaaga
     4321 agggcaagaa ggacgcaatg cgttcaacga gaaacgtgag ccggacttca gcaaattccg
     4381 ccgcaatcca taattgacgg caaatacaag cggttcgatt ttgcaaaaaa tttgcaaaaa
     4441 tcgaccgctt accttttcta atttaagtta attttatgtc agatgttgag ttaaacgagc
     4501 agagcgagca agagcctgag caaaaaccga aaaagcgaaa ctatttcctt tcaaccttat
     4561 tcaaaatcgg ggtgatagga gcttgcagta tggcttttta cggcatttat ttagacggta
     4621 aaatccgtag caaaatggat gggcagatat gggaattacc agctgaggtg tatgctcgta
     4681 ttgaaagcat taaaattgaa gacaacttaa cgcttgagca aaccaaacaa gcgttgctgg
     4741 ataacggcta tcgtcaagtt tctcaagttg cgacaccagg cgatttcaaa attgaagaaa
     4801 atcagctggt gttgctacgc cgagctttcc cgtttccaga aacaccggaa tcgcaacgag
     4861 tgcttcgttt aactttcaaa caagataaat taacttatat tgaagatttg gtgcagggta
     4921 agttgatcga ggagttccgc ttggatccga aattactggc gttactgcat tccgatagcg
     4981 atgaagaacg tcaagcatta cgcttacaac aataccctcg cttgttaatt gagtcactga
     5041 ttttaaccga agatcgccgc ttttatcagc acgatgggat tagcccaatc ggtattgccc
     5101 gtgctttaat cactaactac caagctggca gaacagtgca gggtggcagt accttaacgc
     5161 aacagttagt gaaaaacttg ttcttaagta atgaaagatc gttggtgcgt aaggtaaatg
     5221 aagcgttaat gtcggtgatt ttagacttcc gttacgataa aaaccgcatt ttagaaacct
     5281 atctcaacga aatttattta gggcagaacg gtagctacca aattcacggt tttgcattgg
     5341 caagccaatt ctattttggt cgtccgatcc aagagattag cttagaccaa attgcgttat
     5401 tggtgggaat ggtaaaaggt ccatcactgt ataacccgtg gcgaaatccg caaggtgcgg
     5461 tagagcgacg taatgtggtg ttgcgattat tgctggatca tcaagtgatc aatcaagaac
     5521 tttacgctct gttaagctcc cgcccgctta atattcgtga aaaaggcacg atttaccgtg
     5581 agcaacctgc ctttatgcag gcattaacca ttgatttgaa aaatgagtta ggcgaaatta
     5641 agtcagctca actttccggt actaaaattt ttacgaccct tgaccgtaaa cagcaacgtt
     5701 cagccgaaca agcagtgatt accggtttgg aagatctgga aagtaaaaat aaaaaagtca
     5761 gcgatttgca atcggctatt gtcgtggcgg aataccgcac aggtaaagtg agggcgatag
     5821 taggagaccg cttaacccaa tttgccggct ttaaccgtgc gattcagtca aaacgccaaa
     5881 tcggttcttt agtcaaacca tcggtttatg cgattgcctt ggcacagcct gaattattcc
     5941 gtttaaatac accgatccaa aataaacaaa tttcaattaa ggttggttca acctactgga
     6001 caccccgtaa ctacgaccgc agttttagtg gctcggtgat gttaatggac gccttagtcc
     6061 gctctctgaa tattccaacg gtgaatattg gtatgaaagt tggcttaaaa aaagtcattg
     6121 ccaaacaaaa agagatgggc tgggataatg cggaaattcc gccttaccca tcaaccttgc
     6181 taggttcata ttcgatctcg ccttacgatg taacccgctc ttatcaagta attgccaacg
     6241 aagggctgaa aacaccatta accaccattg aggcgattat ttcgcacaat ggcgaaatgt
     6301 tctatcagcg agatagcggc aaagtgagcg agcaggtgct tccaccggaa gctgcggttc
     6361 aaacaatgta tgcgatgcaa caggttgtgg aacgaggtac ggctcgtagc ttacaaaacg
     6421 attttgccaa actgaaatta gccggcaaaa ccggtacaac caataacgct cgagatactt
     6481 ggtttgtggg aattgatggc gaacacgtca caaccgtctg gctgggtaaa gataacaatg
     6541 gggataccaa cctcaccggt tcaagtggtg cattgtatgt gtataagcaa tatcttgaga
     6601 gagccttgcc tcaggcgtat agattaccga aacttaatac gatgcaatgg gtggggatta
     6661 atagctatgg tagctggaat tgcgatagtg gcagacaaat tccagtttgg cgagataaag
     6721 ggcaacgtta ttgtaccagt gcaggtaatg tagtggctac agaaaaacca agtgtttggg
     6781 atgcactttc gctgaaaaaa gaaaatatcg aggctaaagc agaagaagct gaagggaagc
     6841 aaccaatccc tactgaagaa gttgcaccaa ttgaataaaa ataaatttga tagccattat
     6901 cattcaagat acacaagctg atagattcta cctataaaaa gtatatatta taact
//
LOCUS       DANLXF010000129         6833 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.129, whole genome shotgun sequence.
ACCESSION   DANLXF010000129 DANLXF010000000
VERSION     DANLXF010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6833
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.129"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            41..310
                     /locus_tag="PXD66_001682"
     CDS             41..310
                     /locus_tag="PXD66_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005540356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL6319966.1"
                     /translation="MKIMAVCGSGLGSSFMMEMNIKKVLAKLGVEAEVSHTDLSSVVA
                     DDADLFVMAKDIAASSNVPKEKLIVVKNIVGLAEFEEKLTAYFNQ"
     gene            323..1673
                     /locus_tag="PXD66_001683"
                     /pseudo
     CDS             323..1673
                     /locus_tag="PXD66_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013745980.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
     gene            complement(1768..2916)
                     /gene="dprA"
                     /locus_tag="PXD66_001684"
     CDS             complement(1768..2916)
                     /gene="dprA"
                     /locus_tag="PXD66_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDL6319967.1"
                     /translation="MAYHYDLLRLLQVPQMGAQRIGRLLAELNFAEFCHYDKLQLKQI
                     GWNEKQIQRWFNPDQKWIDQAMTWSELPNQQIVTLFDENYPFLLKQISTAPPLIYVKG
                     NVESLSLPQIAIVGSRDYSHYGEYWAGQFAAELARHNIIITSGLAIGIDGFAHQKIVM
                     EQGITIAVLGSGLNQVYPARHKKLAEQIVDLGGALVSEFSPNQPPVAENFPRRNRIIS
                     GLSLGTLVIEATLNSGSLITARYALEQGREVFALPNAVQNPYAQGCHKLIKEGALLVE
                     GVEDILEVVASQYQPQQIPLFDESKPTVSTSLQAVKNSEKFAKNLPSLTACQQQIYQH
                     ISLEPMAIDDLAKVTELTIEILLVELLGLELAGVIKQVSGGYVMDNGY"
     gene            3005..3517
                     /gene="def"
                     /locus_tag="PXD66_001685"
     CDS             3005..3517
                     /gene="def"
                     /locus_tag="PXD66_001685"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005621146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDL6319968.1"
                     /translation="MAILDVVLYPDEKLATVCEPVEQVDEALNRFIDDMFETMYEHEG
                     IGLAAPQVGVLKRVITIDIEGDKTNQVVLINPEILESSGETGIEEGCLSIPGHRALVP
                     RKEKVKVKALNRQGEEIIIDADDLFAICIQHEIDHLNGVLFVDHISNLKRQRIKEKMQ
                     KLKKQIARAK"
     gene            3611..4537
                     /gene="rfaD"
                     /locus_tag="PXD66_001686"
     CDS             3611..4537
                     /gene="rfaD"
                     /locus_tag="PXD66_001686"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HDL6319969.1"
                     /translation="MIIVTGGAGMIGSNIVKALNEIGRKDILVVDNLKNGEKFINLVD
                     LDIADYCDKEDFIASIIAGDDFGEIDAVFHQGACSATTEWDGKYLMQNNYEYSKELLH
                     FCLDRQIPFFYASSAATYGGRSDNFIEKREFEGPLNAYGYSKFLFDEYVRRILPEAES
                     PVCGFKYFNVYGPREQHKGSMASVAFHLNNQMLKGENPKLFAGSEQFLRDFVYVEDVA
                     KVNLWAWQNSISGIFNLGTGKAESFQEVANAVIKYHGKGEIETIPFPDHLKSRYQTFT
                     QADLTNLRAAGYTGTFKTVAEGTAEYMAWLNR"
     gene            complement(4605..5135)
                     /gene="fabA"
                     /locus_tag="PXD66_001687"
     CDS             complement(4605..5135)
                     /gene="fabA"
                     /locus_tag="PXD66_001687"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017807287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HDL6319970.1"
                     /translation="MNSCTPIIKPSYTYEELLLSGKGELFGEKGPQLPAPPMLMMDRI
                     IEMNQEKGNFEKGYIEAEFDIKPDLWFFNCHFINDPVMPGCLGLDAMWQLVGFYLGWI
                     GGEGKGRALGVGEVKFTGQILPTAKKVTYRIHMKRVVNRKLVMGVADGEVEVDGRVIY
                     TATDLKVGLFQDTTAF"
     gene            complement(5248..>6833)
                     /locus_tag="PXD66_001688"
     CDS             complement(5248..>6833)
                     /locus_tag="PXD66_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL6319971.1"
                     /translation="RLDEKLNFLDFQPQAKQALNQFLQSQFGALLVLKSELFPEFINE
                     ITEYLKKYSVDLPIISKMDFNKNSLFGYSVFLERELKTEYVKGAIHEADKGILIVNIR
                     SLLLDITQWDKLKQALLFGEYEPNSVNHSPYLSAKEKSTFKLILVGEREDISTLSAYD
                     DGLYQFGQYTEIKSYLSLSDNVEQWGGYVQNYAENLINKRLTSTALNQFLQAYIRESE
                     SRELVSISPTLLRKHLLGIQNFYPNVTEIDEVKGYFDYLEQQSSILNQYTVGDILTNQ
                     LYIETEGEEIGQINGLSVIEFEGIPHSFGEPLRMSCNVQYGDGEITDIERKVELGGNI
                     HSKGIIIAQSCLANLLELPTQLPFSASIAFEQSYGEIDGDSSSLAIFNVLVSALAKVG
                     LPQSIAVTGAIDQFGNVLSVGGVNQKIEGFFNICHARQLTGKQGVIIPAACVSHLSLS
                     SEVVEAVEQGLFKIWTVENVFDAIAILFERDFYDDEKAPNSAQTALFTLIHQNLDERE
                     SEETSGSFLSKICKKIKLD"
BASE COUNT         2016 a         1450 c         1315 g         2052 t
ORIGIN      
        1 attcagacaa ctattcattc acctttaatg gagatttact atgaaaatta tggcagtatg
       61 tggttccggc ttaggtagca gctttatgat ggaaatgaac attaaaaaag tattggcaaa
      121 actcggtgtc gaagcggaag tgagtcatac cgacctttcc tctgttgttg ctgatgatgc
      181 tgatttattt gtgatggcaa aagatattgc cgcaagcagc aatgtgccga aagaaaaact
      241 catcgtggta aaaaacattg ttggattagc agaattcgaa gaaaaattga ccgcttactt
      301 taaccaataa cggagatgtt atatggaatc gctactcttt tttatattag atattctcaa
      361 agtgccttcc gttctagtgg gtttaattgc tctggtcggt ttagttgcac agaaaaaatc
      421 ggtatccgat attgttaaag gaacaatcaa aactatttta ggcttcttag tgttaagcgg
      481 tggtgcaaca gtacttttaa gctccctgac tcctttaggc ggtatgtttg aacacgcctt
      541 caacgtgcaa ggcattatcc ctaataacga agcgattgtg tctatggcaa tcgaaaaata
      601 tggtacggcg actgcgttaa ttatggcatt cggtatggta gccaatattt taattgcacg
      661 ttttacccgc ctaaaattca ttttcttaac cggacaccat accttctata tggcgtgtat
      721 gatcggcgta atcctaaccg ttgccggctt tgaaggtatt cagctcgtct ttgtcggggc
      781 attaacctta gggttaatta tgtttcccga ctatcgctca cttctatatg aaaaaagtga
      841 ccgctagcaa tgatgttgcc ttaggtcatt tcggcacatt gggctacgtg cttgctggtt
      901 caatcgggca actggtagga aaaggctcta aatctaccga agaaatggat ttacctaaaa
      961 acctcggctt cttacgtgat agctccattt ctatttcact cacgatgatg gcgatttact
     1021 atattctcgc ccttgcttcg ggcagtgaat acgtttctac attaagcggc ggacaacatt
     1081 atttagtgta tgccaccatt caagccatca cttttgcagc cggtgtttat gtcattttgc
     1141 aaggggttcg tttaatttta gccgagatcg tcccggcatt taccggcttc tccgaaaaat
     1201 tagtacctga tgccaaaccg gctctcgact gcccgattgt tttcccttac gcccccaatg
     1261 cggtattagt tggatttctt gccagtttct taggtgggat tttaagctta gccattctag
     1321 ggcaacttaa ttgggtgtta attttaccgg gcgtagtgcc acacttcttc tgtggggcaa
     1381 ctgccggcgt atttgggaac gccaccggcg ggcgtcgtgg tgcaattatc ggtgctttta
     1441 ttcacggcat attaattacc ttcttgcctg tattcttatt acctgtactg ggttcattag
     1501 gctttgctaa caccactttc tctgactcag acttcggcgg tattgggatt gtgttaggct
     1561 atatggctca atacttcgac aagaatatca ttacgctcat tattgtcggc ttattcgctc
     1621 tgttggtagc ctataattat ttggcgaaaa aaccggcaaa actgaatacc taattaaaat
     1681 caggtgttat ttgctgcctg cgaggaatct tccctgccat ttcttgatgg tagggaattt
     1741 tgtttagtta aacgacaacg ctcttgttca atacccatta tccatcacat accccccact
     1801 cacttgttta atcacaccgg ctaattccag acccagtaat tcaaccagca atatttcaat
     1861 cgtcaactcc gtaactttgg ctaaatcatc aattgccatc ggctctaagc tgatatgctg
     1921 ataaatttgc tgctggcaag cggtcagact tggcagattt tttgcgaatt tttcgctgtt
     1981 tttgaccgct tgtaaagatg tgcttactgt tggcttagac tcatcaaaca aagggatttg
     2041 ctgaggttga tattgcgagg cgaccacctc taaaatatct tccacacctt ccacgagcaa
     2101 tgccccttct ttaattagct tgtggcaacc ttgtgcgtaa gggttttgta ccgcattcgg
     2161 taacgcaaac acttccctac cttgttcaag ggcataacga gcggtaatca gtgaaccact
     2221 attaagcgtt gcttcaataa ctaaagtgcc gagcgataag ccactaataa ttcgattacg
     2281 acgaggaaaa ttttctgcca ccggcggctg attaggcgag aactccgaca ccaatgcacc
     2341 acctaaatca acaatttgtt cggctaattt tttatggcga gcaggataga cttgatttaa
     2401 tccactccct aacacggcaa tcgttatgcc ttgttccata acgatttttt gatgggcaaa
     2461 accgtcaata ccaatggcta aaccgctggt aatgataata ttatggcgag ctaattcggc
     2521 agcaaattgc ccagcccaat attcaccata atgagaatag tctcggctgc caacaatcgc
     2581 aatttgtggc agagaaaggc tttccacatt tccctttaca taaattaacg gtggcgcagt
     2641 cgaaatttgt tttaagagaa acggataatt ttcatcaaag agtgtcacaa tttgctgatt
     2701 tggcaattct gaccaagtca tcgcctgatc aatccacttt tgatcaggat taaaccaacg
     2761 ttgaatttgc ttttcattcc aacctatttg tttgagttga agtttgtcat agtggcaaaa
     2821 ttcagcaaaa ttgagttccg ccaataatcg ccctatacgt tgagccccca tttgcggcac
     2881 ttgtaataat cgtaataaat cgtagtgata tgccatattt tcatcctcaa aatttcccta
     2941 ataataagaa aaggtttaaa atagccaaac gaatttttta aacttttcga gagagatcac
     3001 aaaaatggca atattagatg ttgtgcttta ccccgatgag aaactggcaa ccgtttgtga
     3061 accggtggaa caggtggacg aagcactgaa tcgttttatc gatgatatgt ttgagacaat
     3121 gtacgaacac gaaggtattg gcttagctgc accgcaagtg ggcgtactta agcgtgttat
     3181 tactattgat attgagggcg acaaaaccaa tcaagtggtg ttaattaacc ctgaaatttt
     3241 agaaagcagc ggtgaaaccg gcattgaaga gggctgtctc tccattcccg ggcatcgtgc
     3301 cttagtgcca cgcaaagaga aagtgaaagt caaagccctt aaccgccaag gcgaagaaat
     3361 tatcatcgat gccgatgatt tatttgcgat ttgtattcaa cacgaaatcg accatttaaa
     3421 cggcgtgctt tttgttgacc acatatccaa tttaaaacgc caacgcatca aagaaaaaat
     3481 gcaaaaattg aaaaagcaaa ttgcacgagc gaaataacca ggcacaagcg gttaaaatgg
     3541 cttaaaaatt tgcaaattaa gtgtgatgac acgataataa ccacagttca actttagaaa
     3601 ggataaaaat atgattatcg tcaccggcgg cgcaggtatg attggcagta acattgttaa
     3661 agccttaaat gaaatcggtc gcaaagatat tttagtagta gataacctca aaaacgggga
     3721 aaaatttatc aacttagtgg acttagatat tgcggattat tgcgataaag aggatttcat
     3781 cgcctctatt atcgccggcg atgattttgg tgagattgat gcggttttcc atcaaggggc
     3841 ttgctcggca accaccgaat gggacggcaa atacttaatg caaaataact acgaatattc
     3901 aaaagagtta ttgcattttt gcttagatcg ccaaattcct ttcttctatg cctcaagtgc
     3961 ggcaacctat ggcggacgtt cggataattt tatcgagaaa cgggagtttg aaggtccatt
     4021 aaatgcttat ggctactcca aattcctgtt tgatgaatat gtccgccgta ttttgcctga
     4081 ggcagaatca ccggtctgtg gtttcaaata tttcaacgta tacgggcctc gtgagcaaca
     4141 caaaggttca atggcaagcg tggcattcca cttaaacaac cagatgttaa aaggggaaaa
     4201 tccaaaacta tttgccggct cagagcagtt cttgcgtgat tttgtttatg tggaagatgt
     4261 ggcaaaagtg aatctttggg cgtggcaaaa cagcatttct ggcattttca acctcggcac
     4321 cggaaaagcg gaatctttcc aagaagttgc gaatgcggtt atcaaatacc acggcaaagg
     4381 cgaaattgaa accatcccat tcccggatca tttaaaatcc cgctaccaaa cgttcactca
     4441 agcggactta accaacctaa gagccgctgg ctacacaggc acatttaaaa ccgttgctga
     4501 aggaacagcg gaatatatgg cgtggttgaa tcgctaatca aacgttgtta aaaaagcaga
     4561 aagagctacg aaatgtagct ctttttgtta taaggttata aggattagaa tgctgtcgta
     4621 tcttggaata agcctacttt taaatcagta gcagtataga tcactcgccc gtccacttcg
     4681 acttcaccat cagcaacccc catcactaac ttacgattga ccacacgttt catatggata
     4741 cggtaagtaa cttttttcgc tgtcggtaaa atttgtccgg tgaattttac ttcacctacg
     4801 cccaatgcac gccctttgcc ttcgccacca atccagccga gataaaaacc aaccagttgc
     4861 cacatcgcat ctaagcctaa gcaacccggc attacaggat cgttaataaa atggcagtta
     4921 aaaaaccata aatccggttt aatatcaaac tcagcttcaa tatacccttt ttcaaaattg
     4981 cctttttctt ggttcatttc aatgatgcga tccatcatta acatcggtgg agccggcaat
     5041 tgaggccctt tttcaccgaa taattctcct tttcctgata gcaaaagctc ttcgtaagta
     5101 tagcttggtt tgataattgg tgtacaactg ttcattattt ttaaaatcct gtaaagtgaa
     5161 aaaagcctgt tatatcaata actgataaat caggctgata gtaacagact tttaattcag
     5221 tgaacagttg taagctgact tgtcggatca gtctagcttt atctttttgc aaattttgct
     5281 taaaaatgaa ccgcttgttt cttcactttc ccgctcatct agattctggt gaatcaaggt
     5341 aaataatgct gtttgggctg agttaggggc tttctcatca tcataaaaat cccgctcaaa
     5401 cagaatcgct atggcatcaa acacattttc caccgtccaa attttaaata gaccttgctc
     5461 cactgcctca acaacttctg aacttaaact caaatggcta acacaggccg ccggaataat
     5521 cacaccttgt ttccccgtta attggcgagc gtgacaaata ttaaagaagc cttcaatttt
     5581 ctgatttacc ccgcccacgc ttagcacatt cccaaattga tcgatagcac ctgtcaccgc
     5641 aatagattgt ggcaagccaa ctttagctaa ggcactcact agtacattga aaatcgcaag
     5701 agaagaacta tccccatcaa tttcaccata ggattgttca aaagcaatcg aagctgaaaa
     5761 cggtaactgg gttggtagtt ctaacagatt cgctaaacaa gattgagcga taataatacc
     5821 tttggagtgg atatttcccc ctagctccac tttacgctca atatctgtaa tttcgccatc
     5881 accatattgt acattacaac tcatgcgtaa gggctcgcca aaactatgcg gaatgccttc
     5941 aaactcaatg accgataagc cattgatctg cccaatttcc tctccttcgg tttcaatata
     6001 aagttgatta gtcagaatat cacccaccgt atattgattt aatatcgatg attgctgttc
     6061 taaataatca aaataacctt ttacctcgtc aatttcggtc acatttgggt aaaaattttg
     6121 aattcctaat aaatgttttc tcaataatgt aggggaaata gacaccaact ctcggctttc
     6181 actttcacgt atataggcct gtaaaaattg attaagcgct gtggaagtta atcgcttatt
     6241 gatgagattc tcagcataat tctgaacata acctccccac tgctccacat tatccgataa
     6301 tgataagtag gattttattt cggtatattg cccaaattgg tacaacccat catcataagc
     6361 agataaagta gaaatatctt ctctttcccc tactaaaatc aatttaaatg ttgatttctc
     6421 tttagccgat aaatagggtg agtgattaac agaatttggc tcatattcac caaataataa
     6481 tgcctgtttt agcttatccc attgggtaat atccaataag agcgaacgga tattaacaat
     6541 taaaattccc ttatctgcct cgtgaattgc acctttaaca tattctgttt tcagctctct
     6601 ttccaaaaac acagaataac caaatagact atttttattg aaatccattt tgctaataat
     6661 cggcagatct acactatatt ttttcagata ttccgtgatt tcattgataa attccggaaa
     6721 aagctcagat tttaagacta ataaagcacc aaactgagat tgtaaaaatt gatttaacgc
     6781 ttgttttgct tgaggttgaa aatctaaaaa attcaatttc tcatctaaac gag
//
LOCUS       DANLXF010000130         6641 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.130, whole genome shotgun sequence.
ACCESSION   DANLXF010000130 DANLXF010000000
VERSION     DANLXF010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6641
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.130"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..1995
                     /locus_tag="PXD66_001689"
     CDS             <1..1995
                     /locus_tag="PXD66_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL6319972.1"
                     /translation="EIEQTRAAYDRLKRSGVASQNELRRASEATKQRVRELNAELGKT
                     SFGDKAGSFGRGLMGAGVGIAAGAAMARQPARNQMEFDRQLAMVANTAFSDRDVKGRI
                     DGKRELLDAVQKAVSVGGGSKEEALSSLDALLASGAVSTETALNLLPTLQKGAVATGA
                     SANDMAQIAISSMQQFGIKEEDIGRVLDMAVAAGQAGSFELADMASWLPQQMAAAKQA
                     GLSGIEGFERLLIANQQARVTAGTSNEAGNNLVNLLGKITAKETNERFKNIEYKDPKT
                     GKTKAIDFAKSMEHYKGKGQDSLQAFMSIMDDVVGSDKRYQELQAKLKTAKGEEQKRL
                     FNELTNLVEGTAIGEIISDRQALMALLGIKNNVQLGEKVQKDIQNSEGAVETSHAVIR
                     DTNAHKVESLKNTTEFAQMKNFEKVNGVLGTLAELLNKYANEYPNLTQFLSGATDAVK
                     IFGAALAASSVLDLLTGKKDKGGLIGDVLDKSSKASGGKGKVGKLLSVGGSFASVAGV
                     ATMLHGDQAPMSEERKAELRDPNYQARKMYEYKKEILGSRFHSFFRSNETLEQYRKDV
                     SAYEAKQKNSNTMGAVQGVLSTIPSLQELRPKVDSVTQTLSTYQADFQAFGATISAGI
                     EAGLASQSHTIANQITVELDGSVVAEKVAEYQFNFNKRMA"
     gene            1995..3287
                     /locus_tag="PXD66_001690"
     CDS             1995..3287
                     /locus_tag="PXD66_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HDL6319973.1"
                     /translation="MSGWTMPIQQASFKGVRFDVLAVDDSFERAVVEHAYPFVNGADL
                     EDMGLNTQTIRLQAVFFGSSYYTDYIRLLSVLQQKGADVLVHPIRGRLPNMLLVSANL
                     HTDAENINYVALDLTFKEATEMQPIFAFEDSLISKIDKLLLDLENLFDDGLRFWDSVG
                     VFDLKSRLLGLWGALFASFEAVRNLFDLDKKKYSIASSTSQRTYLRDGSGALNQLKEM
                     VDSGIQGRADLTTLSFKSQLLNITDAIDNTQAIVSKVARDEGFNRSKTVKLLGSDVVE
                     LGIMLDLIATTKLTKVLTELVEEREEELLIGDLELINQIVREHWLNIINKLREQQKNT
                     QKGVPSDKTKVIYETAEKLINNIRNVAHQFTALVLAVINKKPPLTVRMVMDSGTIHKV
                     AHAFYGDYERADELLRLNPHIRQPNFIERGTLLNCYWE"
     gene            3290..4417
                     /locus_tag="PXD66_001691"
     CDS             3290..4417
                     /locus_tag="PXD66_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6319974.1"
                     /translation="MENEIVVEIDGSQHKNWKSYDIDSDFLIPADSFNFELGKSSNIE
                     VLPNYSGKTAIVKINGKTVLTGIVDTTHHTISKSGRGFSLNGRDKAAILVDCSAPITN
                     VQGLSLLDAVKKIVTPLGIKEVQLKAEKNPTLSKVDIDVGESAWEAIMRCANSAGLHC
                     WFDPKGVLIVGGADYAQPPVATLYCVKESGDSRKNNFVDATLSYDVSQSYSEVTFLAQ
                     KHGKDSDNAKHDFKWVYKNEEMELYKPKTIVLGDVENLEALMKQAKKQVSDWQLESFN
                     LTIIVPDHKTKDGTLWQAGQRVHVVCEEYDLDGIFFLMGRRFRLSRTGGTTTELRLKL
                     DGVWTPDAYQAKAQEARKRKGKKGRKKKEPVEYVGSWELGK"
     gene            4419..5081
                     /locus_tag="PXD66_001692"
     CDS             4419..5081
                     /locus_tag="PXD66_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HDL6319975.1"
                     /translation="MMRKLAKQTKEVAKGVQDSVRAAFRGVLNLVKSGSDIQQVQVSA
                     LADETIQDLELMQHFGFTSVPPAGTQAVVIPLGGKTTHGIIVATENGSFRVKNLKNGE
                     TAIYDSSGSTIILKNGRVIDIECDSFNIKCKQYSVTASGGANFNTPKLETDQQFVSQG
                     QISGNGGMVVQGGSGASFTGSLTQQGGSISTDGDVVASGKSLVTHQHNEQGDGRPTSA
                     PI"
     gene            5200..5550
                     /locus_tag="PXD66_001693"
     CDS             5200..5550
                     /locus_tag="PXD66_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015702386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HDL6319976.1"
                     /translation="MDREISPLTRDYTSENITTLQNAVYIRLTTPKGSWWADGRVGSL
                     LHLIQREKDLSRVGLLAQQYAEEALQPIIDDGRAKSITVTHEQPKDGSLNLLISVIDN
                     RGVKFEFKHPVKLV"
     gene            5563..6624
                     /locus_tag="PXD66_001694"
     CDS             5563..6624
                     /locus_tag="PXD66_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDL6319977.1"
                     /translation="MYQIPTLEDVRREILRDIQSLEPTADIEVDSDYFVRASALASCV
                     VGLYAHQNWIVRQYFPDTADSEYLEMHAKLRNLYRKNATYASGSLKVFGTAGSVINEG
                     LQVKFGERFYVTKSSDVIGDDGSATVRVISMATGASSNVKSESKAQLTAAPAGVSSDC
                     LLLPDVTGGTEVESDAALLARLLEQIRRPPSGGNKNDFRQWAESVDGVTSAFVYPLRR
                     GEGTVDIAITSGDTVPSDEIVRATQAYIDEVRPVTAKSVYVLKPVEKRVNFNIKVQLD
                     TDTTLDGVKAEIENALENYFLSLKPADTLIISQIEAVISDLVGVVDREIVQPTRNQTI
                     DAYREMGWFRLGDVTVELM"
BASE COUNT         1955 a         1228 c         1680 g         1778 t
ORIGIN      
        1 gaaattgaac aaactcgtgc agcctatgac cgcttaaaac gctcaggtgt tgcctcgcaa
       61 aatgagctac gccgtgcctc agaagcgact aagcaacgag tccgagagtt aaatgctgaa
      121 ttgggtaaaa ccagttttgg ggataaggca ggaagttttg ggcgtgggtt gatgggtgca
      181 ggggttggaa ttgctgcagg tgcagcaatg gctcgccagc ctgcaagaaa tcaaatggaa
      241 tttgatagac agcttgcaat ggtagcgaat accgcctttt cagatcgtga tgtgaaaggg
      301 cgtattgatg ggaaaagaga gttacttgat gccgtacaga aagcagtaag tgtaggcggt
      361 ggctcaaaag aagaagcgtt gagctcacta gacgctttac ttgcctcagg tgctgtttct
      421 accgaaaccg ctctcaattt actcccaacg ttgcagaaag gtgcggtcgc aacaggggca
      481 agtgcaaatg atatggctca aattgctatt tcctcaatgc aacaatttgg gattaaagaa
      541 gaggatattg gtcgagtctt agatatggca gttgccgctg ggcaagccgg tagctttgag
      601 cttgcagata tggcaagttg gttgcctcaa caaatggccg ctgcaaaaca agctggctta
      661 agcggcattg agggatttga gcgactttta attgcaaacc aacaggctcg tgtcactgcc
      721 ggtacaagta acgaggctgg taataattta gtgaatctgt tgggtaagat tactgctaaa
      781 gaaactaatg agcgttttaa gaatattgaa tataaagatc cgaaaactgg caaaaccaaa
      841 gcgattgatt ttgcaaaatc tatggagcat tacaagggaa aagggcaaga ttcattgcag
      901 gcttttatgt ctattatgga tgacgttgtc gggagtgata aacgctatca agaactacaa
      961 gctaaactta aaactgctaa aggcgaagag cagaaacgct tattcaatga attaacaaac
     1021 ttagttgaag gtacggcaat tggagaaatt atttctgatc gccaagcatt aatggctcta
     1081 ttagggataa agaataatgt tcaattaggt gaaaaagtac agaaagatat tcagaatagt
     1141 gaaggagctg ttgaaacctc tcacgctgtt attcgggata ctaatgctca taaagttgaa
     1201 tcattaaaaa atacaactga atttgcacaa atgaaaaact ttgaaaaagt aaatggagtg
     1261 ttaggcacat tagctgaatt actgaataaa tacgccaatg aataccctaa cttaactcaa
     1321 tttttaagtg gtgcaacaga tgccgttaaa atttttggag cagctcttgc tgcatcaagt
     1381 gtgcttgatt tattaaccgg taaaaaagac aagggcggac taattggtga tgttttggat
     1441 aaatcatcaa aagctagtgg aggtaaaggt aaggtaggta aattgctttc ggttggtggc
     1501 tcttttgcta gtgttgcagg cgttgccaca atgctacacg gcgaccaagc cccgatgagt
     1561 gaggagcgaa aagcagagtt aagagatcct aattatcaag ctcgtaaaat gtacgagtac
     1621 aaaaaagaga ttttaggtag ccgtttccac tcgttttttc gtagtaatga aacccttgag
     1681 caatatcgga aagatgtgag tgcttatgag gctaagcaaa agaatagcaa tactatggga
     1741 gctgtacaag gggttttaag taccattccg agcttacaag agttacgccc taaagttgat
     1801 tcggttactc aaaccctatc cacctatcaa gccgactttc aggcttttgg ggcaacgatt
     1861 tcagccggta ttgaggcagg gcttgcctcg caatctcata cgattgcaaa tcaaatcacc
     1921 gtggagctgg acggttcggt ggtggctgaa aaagtggctg aatatcagtt taattttaac
     1981 aagaggatgg cgtaatgagc ggttggacaa tgcctattca acaggcgagt tttaaaggtg
     2041 tgcgttttga cgtgcttgca gtggatgata gctttgagcg agcggtagtt gaacacgcct
     2101 acccttttgt aaatggggct gatttagagg atatggggct aaacacccaa actattcggc
     2161 tgcaagcggt cttttttggc tcaagctatt acacggacta tatccgctta ctttcagttt
     2221 tgcagcaaaa aggggctgat gtattagtcc accctattcg tgggcgttta ccgaatatgt
     2281 tactcgtttc ggcgaatcta cataccgatg ccgaaaatat caattatgtt gccttagatt
     2341 tgacctttaa agaggcaacc gaaatgcagc cgatttttgc ctttgaagat tcgctgattt
     2401 cgaaaatcga caagctgcta cttgatctcg aaaacctgtt tgatgatggc ttgcgttttt
     2461 gggatagcgt tggggtgttt gatctgaaat ctcgtttgct tgggctttgg ggggcgttgt
     2521 ttgcctcgtt tgaggcggtg cgtaacctgt ttgacttgga caagaaaaaa tacagcattg
     2581 cctcatcaac ctcacaacgc acctatttaa gagacggttc gggggcattg aatcagttaa
     2641 aagaaatggt ggatagcggt attcaggggc gtgctgattt aacgacactc tcgtttaaat
     2701 ctcagttact caatattact gatgcgatag ataacactca ggcgattgtg agcaaagtgg
     2761 ctagagatga gggttttaac cgctcaaaaa cggttaagtt acttggctct gatgtggttg
     2821 agcttggcat tatgcttgac cttattgcca cgactaagct caccaaagtc ttgactgagc
     2881 tggtagaaga gcgtgaggaa gagctgctaa ttggtgattt agagttaatt aaccaaattg
     2941 tgcgtgagca ttggctgaat attatcaaca aactgcgtga gcaacagaag aacactcaaa
     3001 aaggcgtgcc gagtgataag acaaaagtta tctacgagac cgcagaaaag ctgatcaaca
     3061 atatccgtaa tgttgcccat cagtttactg cacttgtgct tgcggtaatt aataaaaaac
     3121 cgcctttgac cgtgcgaatg gtaatggata gcggcacaat ccataaagta gctcacgcct
     3181 tttatgggga ttatgagcga gcggatgagc tattacgtct taatccgcat attcgccagc
     3241 caaactttat tgagcgaggc acgctgttaa attgttattg ggagtaaaaa tggaaaatga
     3301 aattgtggtg gagattgacg gctcgcagca taaaaactgg aaaagttatg atattgacag
     3361 cgatttttta attcctgctg atagttttaa ttttgagttg ggtaaatcta gcaatataga
     3421 ggttttacct aattattcgg gcaaaacggc aatagttaaa atcaatggca aaacggtgct
     3481 cactggcatt gtggatacca cacatcacac cattagcaaa agcgggcgtg ggtttagcct
     3541 aaatggacgt gataaagccg ctattttggt ggattgctct gccccgatta ccaatgtgca
     3601 agggctctct ttgcttgatg ctgttaaaaa aattgtaacc cctcttggga ttaaagaggt
     3661 gcaactgaaa gccgagaaaa acccgacttt aagcaaggtg gatattgatg tgggtgaatc
     3721 ggcgtgggag gcgattatgc ggtgtgccaa ctcggcaggt ttgcattgtt ggtttgaccc
     3781 caaaggcgtg ttaattgtcg gcggtgcgga ttacgctcaa ccgcccgttg ccacgttata
     3841 ttgcgtgaaa gagtcggggg atagccgtaa aaataacttt gttgatgcaa cactttctta
     3901 tgatgtgtcg caaagctatt cggaggtcac ttttcttgct caaaaacacg gcaaagattc
     3961 tgataatgcc aagcacgatt ttaaatgggt gtataaaaat gaggaaatgg agctttataa
     4021 accgaaaact atcgtgctgg gcgatgtgga gaacctcgaa gccttgatga aacaggcgaa
     4081 aaaacaggta tcagattggc agttggagtc atttaacctc acgattattg tacctgacca
     4141 taaaaccaaa gacggcacgt tgtggcaagc tgggcaacgt gtgcacgtgg tatgtgagga
     4201 gtatgattta gatggtattt tctttttaat ggggcgacgt tttcgactct ctcgcacggg
     4261 tggcacaacc accgaattgc ggttaaaact ggatggcgtg tggacacctg atgcttatca
     4321 agctaaagca caagaggctc gcaagcgtaa aggcaaaaaa ggacgtaaga aaaaagagcc
     4381 tgtagaatat gtaggctcgt gggagttggg aaaataatat gatgagaaaa ttagcaaaac
     4441 agactaaaga ggtggcaaaa ggtgtgcaag atagcgtgcg tgctgctttt agaggggtat
     4501 taaatttagt caagagtggc agtgatattc agcaggtaca ggtgtccgct ttagcggacg
     4561 aaactatcca agatttagag ctaatgcagc attttggttt cacttccgtg ccacctgccg
     4621 gcacgcaagc ggttgttatc cccttaggtg gtaaaaccac gcacggtatt attgtggcga
     4681 ctgaaaacgg ctcgtttagg gttaaaaatc taaaaaatgg tgagacggct atttatgatt
     4741 caagcggctc aactatcata ttaaaaaacg ggcgagtgat tgatattgag tgcgatagct
     4801 ttaacattaa atgcaagcaa tattccgtca ccgcaagcgg tggagcaaat tttaacaccc
     4861 cgaaactaga aaccgaccag cagtttgtgt ctcaagggca gatcagcggc aacggtggaa
     4921 tggtagttca aggcggaagt ggtgcaagtt tcactggctc attaacacaa caaggaggca
     4981 gtatctcaac tgatggtgat gtggtggcat cgggtaaatc actggttaca catcaacaca
     5041 acgaacaagg cgatggcaga cctacctctg ctccaattta acttacaagc taatttacaa
     5101 gcggtctaat tttgcaaatt ttttgtagaa aaagaccgct tgccatttgt ggaagtctct
     5161 ccccgttact cctgtcttta atgtctctac catagcaata tggacagaga aatcagcccg
     5221 ctcactcggg actatacaag tgaaaatata actacactgc aaaatgccgt gtatatcaga
     5281 ctaactactc cgaaaggctc gtggtgggca gatgggcgtg taggttctct gctccatctt
     5341 attcaacgtg agaaagattt aagccgtgtg gggctattag ctcaacagta cgccgaagag
     5401 gctttgcagc cgattattga tgacggacga gccaaaagca ttacggtgac acacgagcag
     5461 ccaaaagacg gctcgttaaa tcttttaatc tccgttattg ataatcgtgg ggttaaattt
     5521 gagtttaaac accctgtaaa actggtttag gtggttttta aaatgtatca aatcccaacg
     5581 cttgaagatg ttagaagaga aattctgcga gatattcaat ctttagagcc aactgctgat
     5641 attgaggtgg attcggatta ttttgttcgt gcctccgctt tggcaagctg tgtggtgggg
     5701 ctgtatgctc accaaaactg gattgttcgt cagtatttcc cagatacggc agactccgaa
     5761 tacctcgaaa tgcacgctaa attgcgaaat ctttaccgta aaaatgcgac ctatgcgagt
     5821 ggttcgttga aagtttttgg tactgctggc tcagtaatca atgagggctt gcaagttaaa
     5881 tttggcgagc gtttttatgt aaccaaaagc tcggacgtta ttggtgatga tggctctgca
     5941 acggtgcgag tgatttcaat ggcaacaggg gcatcaagca atgttaaatc tgaaagtaaa
     6001 gcacaactga ctgctgctcc tgctggcgta tcaagcgatt gcttgttatt gcctgatgtg
     6061 acaggcggga cggaagtgga aagcgatgcg gcattgctgg ctcggttatt agagcagatt
     6121 cgccgaccac cgtcaggcgg gaataaaaat gacttccgcc aatgggcaga aagcgttgat
     6181 ggcgtaacct ctgcgtttgt ttatccatta cggcgtggcg agggtacggt agatattgca
     6241 atcacctcag gtgataccgt gccaagtgat gaaatcgtgc gagcaactca agcttatatt
     6301 gatgaagtcc gccctgttac ggctaaatcg gtttatgtac ttaagccagt tgaaaaacgg
     6361 gtcaatttta acatcaaagt gcaacttgat acggatacga cgcttgatgg tgtaaaagcg
     6421 gaaatcgaaa acgctttaga gaattatttt ttatcactca aacctgctga caccttaatt
     6481 atttcgcaaa ttgaggcagt aattagtgat ttagtgggcg tagtagatcg tgagattgta
     6541 cagccaacca gaaaccaaac gattgatgcc taccgtgaaa tgggctggtt cagattgggt
     6601 gatgtaactg tggagttgat gtgatggtaa cgattaactc t
//
LOCUS       DANLXF010000131         6470 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.131, whole genome shotgun sequence.
ACCESSION   DANLXF010000131 DANLXF010000000
VERSION     DANLXF010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6470
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.131"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..1828
                     /locus_tag="PXD66_001695"
     CDS             <1..1828
                     /locus_tag="PXD66_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HDL6319978.1"
                     /translation="IVATDSQWNTPLAIFWTLVLCLPSTLMFLAGFVAITYISFENPQ
                     EVWTWGLKMAIYWLYFAFLVAMLRPHGIGHIHFNMPQKSNEIFRNILKRSVWIIALLL
                     NTSIFTNLEMGISYDMLGQTMTIFVLILMIWMIVPAFRQAISTYENVTTEEKKESKSI
                     LLTIVKLVLLLAPIILIILIALGYYYTALVIIEHLVDSYFAIITWIIIRNILYRAFSI
                     SSRRLAYRRLQEKREAAEAKTRAKAEQNDENPDGVELPFELKEDSIAVSEVKNQMLKL
                     TDFVLWIGLFVLLYWVWSDLITVALYLDGVTLWQQSVTTEAGIVMESITLLNLLTAIL
                     IIVVMVVLIRNLSGLLEALIFSHFKLSQGTPYTITTLLTYLIIALGAIFAFGTLGMSW
                     SKLQWLLTALSVGLGFGMQEIFANFVSGIIILFERPMRIGDTITIGTYSGTVSKIRIR
                     ATTLVDFDGKEVIVPNKAFVTERLVNWALSSTATRIIVQVGVAYGSDLELVKRLLLQV
                     AEENPKVLKDPQPRAYFLTFGASTLDHELRAYVNDLSDRNPTIDALNTRIHQLFNEHN
                     IDIAFNQLDVYIKNNDTQDEIKVDSKRFEFERPNNITAKEKE"
     gene            1831..2913
                     /gene="aroC"
                     /locus_tag="PXD66_001696"
     CDS             1831..2913
                     /gene="aroC"
                     /locus_tag="PXD66_001696"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012072711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDL6319979.1"
                     /translation="MAGNSIGQLFKVTTFGESHGIALGCIVDGVPPNMALSEADLQPD
                     LDRRKPGTSRYTTPRREDDEVQILSGVFEGKTTGTSIGLIIKNGDQRSQDYGDIAEKF
                     RPGHADYTYQQKYGIRDYRGGGRSSARETAMRVAAGAIAKKYLREQFGIEVRGYLSQI
                     GNIKIDPKTVENIEQIDWHAVNSNPFFCPDPVAIEGFDALIRELKKEGDSVGAKLTVI
                     AERVPVGLGEPVFDRLDADLAHALMSINAVKAVEIGDGFAVVEQRGSQHRDEMTPQGF
                     LSNHAGGILGGISSGQPIIAHIALKPTSSITIPGRSVNKNNEAVEVITKGRHDPCVGI
                     RAVPIAEAMMAIVLLDHLLRFKAQCR"
     gene            2932..3759
                     /gene="mepA"
                     /locus_tag="PXD66_001697"
     CDS             2932..3759
                     /gene="mepA"
                     /locus_tag="PXD66_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HDL6319980.1"
                     /translation="MKLLKTLLVSSLAFTATALNATQWEKIKTPVQGKAQSIGGYSNG
                     CIIGAQPLALKGEGYQVIRSARNRYYGHPQLLDYLTHLGKKTKAAGVPTILIGDMGMP
                     AGGRFASGHASHQTGLDADIWFKFGPMDDETARNPAGLATIMVNRSTERVDDSLWNQH
                     HTNLVKLAATDPRVDRIFVNPAIKVKLCQTAGNDRAWLRKIRPWYGHDSHFHVRLTCP
                     ADAASCENQALVPAGDGCGEELYSWFEPKEASGTPKAKVLPTAPVQCQMILSEQGLN"
     gene            3832..5751
                     /locus_tag="PXD66_001698"
     CDS             3832..5751
                     /locus_tag="PXD66_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL6319981.1"
                     /translation="MRLFVAEKPSLARAIADVLPKPHTKGDGFIQCGEHDTVTWCIGH
                     LLEQAEPDVYDERFKMWRMEHLPIVPEQWKLIPKKETLKQFKVVEKLIKKADVLVNAG
                     DPDREGQLLVDEVFGYLNLSAERKSQIQRCLVSDLNPNAVSRAIEKLQKNSDFIPLAT
                     SALARARADWLYGINMTRAYTLQGRWAGYKGVLSVGRVQTPVLGLIVRRDLEIEHFVP
                     KDYFEVLAQIVVPETQERFKAQWQPSKACEDYQDEDGRVLSRPLAENVVKRITDQPAT
                     VTEYQNKIEKETAPLPYSLSVLQMEAAGRYGLSAQAVLDICQKLYETHKLITYPRSDN
                     RYLPTEHFNDRFKVMAAIAHHLPEYQQKPEVIDATIRNRCWNDKEVEAHHAIIPTARQ
                     GTVHLTENEKRVYQLIARQYLMQFCPDAEYRKSKIGLEIAGGSFVAQARNLVIAGWRA
                     LLGKEDSLDDAEPLLPIVKKGQILHCEKGEIVSKKTQPPRHFTDRSLLSAMTGIARFV
                     QDKELKKILRETDGLGTEATRAGIIELLFKRGFLIRKGKNIHSTEAGRILIQALPDSA
                     TQPDMTAHWEMQLNQISKKETSYQQFMFDLNQQLPHLLQSPNRQVLQNLAKITPLASG
                     NKPRYFKKRTQTAEN"
     gene            5857..6183
                     /gene="secG"
                     /locus_tag="PXD66_001699"
     CDS             5857..6183
                     /gene="secG"
                     /locus_tag="PXD66_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDL6319982.1"
                     /translation="MLINVLTIVYLVVAVLLIAFVLMQQGKGADAGASFGAGASATVF
                     GSAGSANFLSRTTALLATIFFAISLVIGNLSSHNVAPKSQFEDLSQVEQKQVEPVKIE
                     NSDIPQ"
     gene            6208..6293
                     /locus_tag="PXD66_001700"
     tRNA            6208..6293
                     /locus_tag="PXD66_001700"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6242..6244,aa:Leu,seq:gag)
BASE COUNT         1917 a         1412 c         1417 g         1724 t
ORIGIN      
        1 aattgtcgcc acagatagcc aatggaacac ccctcttgct attttttgga cattagtgct
       61 ttgcctgcca agtacactaa tgttcttagc cggatttgtt gctatcactt atatttcatt
      121 tgaaaatcct caggaagtct ggacttgggg attaaaaatg gcaatttatt ggctgtattt
      181 tgccttttta gtggcgatgc tacgcccgca cggcatagga catattcact ttaatatgcc
      241 acaaaaaagt aatgaaatct tccgtaatat tttaaaacgt tcggtatgga tcattgcttt
      301 attactgaac acctctattt ttacaaactt agagatggga atatcttacg acatgcttgg
      361 acaaacaatg actatttttg tccttatatt aatgatttgg atgattgtac cggcatttag
      421 gcaagctatt tctacctatg agaatgtcac aacagaagag aaaaaagaat ctaaaagtat
      481 tttactcact attgtcaaat tagtgctact gcttgcccct atcattctta tcattctgat
      541 tgcattgggc tattactata ctgcactggt gatcattgag catttagtag attcttattt
      601 tgcgatcatt acttggatta ttattcgtaa tattctctat cgagcattct ctatttcatc
      661 acgccgtctg gcttacagac gtttacaaga aaaaagagaa gcagctgaag ccaaaacacg
      721 agctaaagca gaacaaaatg atgaaaaccc ggacggtgtc gagcttcctt ttgagctaaa
      781 agaagactct attgccgtaa gcgaagtcaa gaaccaaatg ttgaaattaa ctgattttgt
      841 gctttggatt ggcttatttg tgctgcttta ctgggtttgg tcggacttaa ttactgttgc
      901 actctaccta gacggcgtta ccttatggca acaaagtgtt acaacagaag ccgggattgt
      961 aatggaatcc attaccctgc tgaatttact cactgctatt ctgattattg tggtaatggt
     1021 ggtactcatt cggaacttat ccggtttact tgaagccctg attttctcgc acttcaagtt
     1081 atcgcaaggt acaccataca caattaccac actcttaacc tatttgatta ttgcgttagg
     1141 ggcaatcttt gcgttcggta cattagggat gtcttggagc aagctccaat ggctactaac
     1201 tgcactttct gtcggtctgg gttttggtat gcaagagatc tttgcaaact tcgtttcagg
     1261 gattatcatc ttgtttgaac gtccaatgcg gattggcgac accattacca tcggcactta
     1321 cagcggtacg gtttccaaaa ttcgtattcg tgccacaacc ttagtcgatt ttgacggcaa
     1381 agaagtgatt gtaccaaaca aagcattcgt aaccgaacgc ttggtgaact gggcattaag
     1441 cagcaccgca acccgcatta ttgttcaagt cggcgtggct tacgggtctg atttagaatt
     1501 agtcaaacgg ctgttattac aagtggcgga agaaaatccg aaagtcttga aagatccaca
     1561 accaagggct tatttcctta cctttggtgc aagcacttta gatcacgaat taagagccta
     1621 tgtgaacgat ttatctgatc gtaacccaac tattgatgca ctaaatacac gcatccacca
     1681 gctctttaat gaacataata ttgatattgc atttaaccaa ttagatgttt atatcaaaaa
     1741 caacgataca caagatgaaa ttaaagtaga ttcaaaacgc tttgagtttg aacgaccaaa
     1801 taacataaca gcaaaagaaa aggaataaca atggcaggca acagcatcgg acaattattt
     1861 aaagtgacca ctttcggcga atctcacggt attgcattag gctgtatcgt agatggcgta
     1921 ccgccgaata tggctttaag cgaagcagat ttacagccgg atttagaccg ccgtaaaccc
     1981 ggtacttcac gctacaccac gcctcgccgt gaagatgatg aagtgcaaat tttatcggga
     2041 gtctttgaag gcaaaacgac gggaacaagc attggtttaa tcattaaaaa cggtgaccaa
     2101 cgctcacaag actatggcga cattgccgaa aaattccgcc cggggcacgc agattatacc
     2161 tatcagcaaa aatacggcat tcgagattac cgtggcggtg ggcgttcttc tgctcgggaa
     2221 accgcaatgc gggttgccgc cggggcgatt gccaaaaaat atttgcgtga gcaatttggc
     2281 attgaagtgc gaggctacct ctcacaaatc ggcaatatca aaattgaccc gaaaaccgtt
     2341 gaaaatattg aacaaattga ttggcacgca gtcaatagta atcctttctt ctgccccgat
     2401 cccgttgcga ttgaaggctt tgatgcctta attcgtgagc tgaaaaaaga gggcgattcg
     2461 gtgggggcaa aattaaccgt gattgcagag cgtgtacctg tagggttagg cgaacctgtg
     2521 tttgaccgcc tagatgccga ccttgcccac gccttaatgt cgatcaacgc cgtgaaagcg
     2581 gtggaaattg gcgatggttt tgcagttgtg gaacaacgtg gtagccagca ccgagatgag
     2641 atgacgccgc aaggctttct ctctaatcac gccggcggaa ttttaggtgg tattagctcc
     2701 ggtcagccga ttatcgctca tattgcgtta aagccgacct caagcattac tattcccggc
     2761 agaagcgtga acaaaaacaa cgaagccgtt gaagtgatca ctaaaggcag acacgacccc
     2821 tgtgtaggta ttcgtgctgt gccgattgca gaagcgatga tggcgattgt actactcgac
     2881 catctgttaa gatttaaggc tcaatgccgt taaacacata aaattggaaa aatgaaatta
     2941 ttaaaaacac ttttagtctc aagccttgct tttacagcta ccgcactgaa tgcaacgcaa
     3001 tgggagaaaa tcaaaactcc ggttcaagga aaagcacaat ctatcggcgg ttatagtaac
     3061 ggctgtatta ttggggcaca gccccttgca ttaaaaggcg aaggctacca agtgatccgc
     3121 tcggctcgta accgctatta cggacaccca caactgctcg attatctcac tcacttaggc
     3181 aaaaaaacca aagctgccgg tgtgccgacc attttaatcg gcgatatggg aatgcctgcc
     3241 ggtgggcgtt ttgcctccgg tcacgcctcc catcaaaccg ggctggatgc tgatatttgg
     3301 tttaagttcg gaccgatgga cgatgaaacc gcccgcaatc cagcagggct tgccaccatt
     3361 atggtaaacc gtagtaccga gcgggttgat gattcactct ggaatcaaca ccatactaat
     3421 ttagtaaaac ttgctgcaac cgatcctcga gtggatcgca tttttgtcaa tcctgccatt
     3481 aaagtgaaac tctgccaaac cgcaggcaat gatcgtgctt ggttacgcaa aattcgcccg
     3541 tggtacggac acgactccca tttccacgtc cgtttaacct gcccggctga tgccgcaagt
     3601 tgtgaaaatc aagcccttgt gcctgccggt gacggctgtg gtgaagagct ttactcgtgg
     3661 tttgagccga aagaagcctc cggcacacca aaagccaaag tgctacccac agcgcctgtt
     3721 caatgtcaga tgattttatc tgaacaaggc ttgaattaat tgcaaaataa caaccttttt
     3781 tgaccgcttg tcagcccaca agcggttcat tttcttaaat aatttacgat tatgcgttta
     3841 tttgttgccg aaaaacccag ccttgcccga gcgattgccg atgtgttacc aaaaccccat
     3901 accaaaggag atggctttat ccaatgcggc gaacacgaca ctgttacttg gtgtatcggt
     3961 catttattgg aacaggctga acccgatgtc tatgatgagc gtttcaaaat gtggcgaatg
     4021 gaacatttgc cgattgtgcc tgaacaatgg aaattgattc ccaaaaaaga gacgctcaag
     4081 cagtttaaag tggtggaaaa gctgattaaa aaagccgatg ttttagtcaa tgccggagac
     4141 ccggatcgag aagggcagct gttggtggac gaagtttttg gctatttgaa tttatctgct
     4201 gaacgcaaaa gccaaattca gcgttgtttg gtaagcgatc tcaacccgaa tgcggtgagc
     4261 cgagccattg aaaaattgca aaaaaacagc gattttatac cgcttgcaac ctctgctctc
     4321 gcccgagcca gagcggattg gctgtatggt atcaatatga cccgagccta taccctacag
     4381 gggcgttggg caggctataa aggcgtgtta tcagtcggac gtgtgcaaac gccggtatta
     4441 gggctgattg ttcgccgaga tctggagatc gagcatttcg tgccaaagga ttattttgag
     4501 gtgttggcac agattgtcgt gccggaaacc caagagcgat ttaaggcgca gtggcaaccg
     4561 agcaaagcct gtgaagatta tcaagatgaa gatgggcggg tgctgtctcg tccattggca
     4621 gaaaatgtgg taaaacggat taccgaccag ccggcaaccg tcaccgaata tcaaaataaa
     4681 attgaaaaag aaactgcacc gctgccttat tctctttcgg tattacaaat ggaagccgcc
     4741 ggcagatatg ggctatccgc ccaagcggtg ttggatattt gccaaaagct gtatgaaacc
     4801 cataaactaa ttacctaccc tcgttctgat aatcggtatt tgccgaccga acatttcaac
     4861 gacagattta aagtgatggc ggcgattgct catcatttac ctgaatatca acaaaaaccg
     4921 gaagtgattg atgccactat tcgcaatcgt tgctggaatg ataaagaggt ggaagcccac
     4981 cacgccatta ttcccactgc acggcaaggg acggtacatt taaccgaaaa tgaaaaaaga
     5041 gtttatcaac tcattgcccg ccaatatcta atgcaattct gccctgatgc ggaatatcgc
     5101 aaaagtaaaa tcgggcttga aattgcaggt ggtagttttg tcgctcaagc acgcaacctt
     5161 gtaattgccg gctggcgagc gttactgggt aaagaagata gccttgacga tgcagagcca
     5221 ctactaccga ttgtcaaaaa agggcaaatc ctccattgtg aaaaagggga aattgtcagc
     5281 aaaaaaacac aaccgccccg ccactttact gatcgcagtt tgctttcagc aatgaccggg
     5341 atcgcccgtt ttgtacaaga taaagaattg aaaaaaatct tgcgtgaaac cgatggttta
     5401 ggcacggagg caacccgagc cggtattatc gaattgctgt ttaaacgtgg ttttttaatc
     5461 cgtaaaggga aaaatatcca cagtaccgaa gccggcagaa tattgattca agcacttccc
     5521 gactctgcta cccagccgga tatgaccgct cattgggaaa tgcagctgaa tcaaatcagc
     5581 aaaaaagaga ccagctatca gcaatttatg tttgatctaa accaacagct cccacattta
     5641 ttacaaagcc caaaccgcca agtgttgcaa aatttagcga aaatcacacc gcttgcaagc
     5701 ggcaataaac ctcgttattt taaaaagcga acacaaactg ctgaaaatta aatcaagcag
     5761 gtaaaaatta ctgtttagca cttgtcaaac gggcgtaaat tctatattat gcacgccatt
     5821 gcaaatttcg cataaagaat acaagagaat aaattaatgt taattaatgt actcactatt
     5881 gtttatcttg tagttgccgt attactgatc gcatttgtat taatgcaaca aggtaaaggc
     5941 gctgatgcag gtgcttcttt cggggcgggt gcttcagcaa cagttttcgg ctcagcagga
     6001 tcagcaaatt ttttatcaag aacaactgct ctacttgcaa cgattttctt cgcaattagt
     6061 ttagttatcg gtaatttaag ctcacacaat gtagcaccga aaagccaatt tgaagactta
     6121 agccaagttg aacaaaagca agtagaacct gtaaaaatag aaaatagcga tatccctcaa
     6181 taatcgctta acattaaaaa gaataaagct ctggtggtgg aattggtaga cacgccatct
     6241 tgagggggtg gtggacacag tccgtgcgag ttcaagtctc gcccagagca ccattcaact
     6301 aaatttggct caacggagct aaactaaaaa agccttagaa agcctgtaaa atcaacattt
     6361 acaggctttt ttactatctt aactcaacgg ctctaaacgt ttcctatcac aaattttagt
     6421 tacacgttta gttacacgtt ttaaatcgtg ttactatact ttcaataatc
//
LOCUS       DANLXF010000132         6463 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.132, whole genome shotgun sequence.
ACCESSION   DANLXF010000132 DANLXF010000000
VERSION     DANLXF010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6463
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.132"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..391)
                     /locus_tag="PXD66_001701"
     CDS             complement(<1..391)
                     /locus_tag="PXD66_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL6319983.1"
                     /translation="MSINISPLASKNLTEWKLLWQQYLDFYQVNLADDIIQSTWQKVS
                     ESENVKGFGAFVDNRMVGFVHIVIHPNTWNLTECCYLEDLFVLAEYRNQGIGKELIEY
                     IYRYAQEKGYNRVYWVTDKNNHQAQQLY"
     gene            complement(458..835)
                     /locus_tag="PXD66_001702"
     CDS             complement(458..835)
                     /locus_tag="PXD66_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HDL6319984.1"
                     /translation="MKNPIEIYQSQDGKTQVSVQFDKETVWLSLQQMADIFGRDKSVI
                     SRHLRNIYQEGELEREATVAKNATVQIEGAREVVRLKDYLVKGYALNERRLQERGIEF
                     EQVIGLLSQYTGCLSLVGGRKFA"
     misc_feature    complement(857..979)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            complement(1109..2098)
                     /gene="rsmC"
                     /locus_tag="PXD66_001703"
     CDS             complement(1109..2098)
                     /gene="rsmC"
                     /locus_tag="PXD66_001703"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HDL6319985.1"
                     /translation="MLSLESEVLERHLALFENKSVLLFGDVRDDFARQLQSRAEKVAV
                     FSSYFDYARHHSEVEFGLECGLSAELAVFYWTKNKQECQFQLLQWLSQCQAGQEMLII
                     GENRAGVRSVEKLLEPFGNIAKIDSARRCGLYHFELQTVPTFDCKKFWKSYRLQDLDV
                     FDLPAVFSSAELDNGTKLLLSTFDKADRLKGKVLDLGCGAGVIGATLKQQFPKIKLTM
                     SDIHAMALESGRRTLAENQLEGEVVASDVFSHINERFDLIVSNPPFHDGVDTAYRAVE
                     ELIFQAKNHLTKGGELRIVANAFLPYPDLLDKAFGKHDVLAKSTKFKVYSART"
     gene            2228..2650
                     /locus_tag="PXD66_001704"
     CDS             2228..2650
                     /locus_tag="PXD66_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HDL6319986.1"
                     /translation="MNRRDLLLQEMGISQWVLTNPQVLKGDAQIRLNNAVKLVVVCEE
                     DHQHSRLFADILRALKLKNSEYQWLTGEQSQRLVFEHSPIFWLIQAEEQAVKMAKSFT
                     NQTAWKNESWQDLAKSTTKRQLWQQIDTFSAQFGEQRD"
     gene            2643..3080
                     /gene="rimI"
                     /locus_tag="PXD66_001705"
     CDS             2643..3080
                     /gene="rimI"
                     /locus_tag="PXD66_001705"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDL6319987.1"
                     /translation="MIEQIQAEDFEQLFEIEQQAHLVPWSKGTLLNNQGEKYLNLKVV
                     EQNKIVAFAISHIVLDEATLFNIAVAPDFQGKGIGKRLLSELILQLQQRKLTTLWLEV
                     RESNHSARKLYDSLGFNEVTTRKNYYPTPNGSKENAVVMALYL"
     gene            complement(3129..4976)
                     /gene="typA"
                     /locus_tag="PXD66_001706"
     CDS             complement(3129..4976)
                     /gene="typA"
                     /locus_tag="PXD66_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDL6319988.1"
                     /translation="MQNVDISKLRNIAIIAHVDHGKTTLVDKLLQQSGTFGETRGDVD
                     ERVMDSNDLEKERGITILAKNTAINWNGYHINIVDTPGHADFGGEVERVLSMVDSVLL
                     VVDAFDGPMPQTRFVTQKAFAHGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLGASD
                     EQLDFPIIYASALNGVAGLEHEDLAADMTPLYEAIVKHVQPPQVELDAPFQMQISQLD
                     YNNYVGVIGIGRIKRGSVKPNQAVTIVDSEGKTRQGRIGQVLGHLGLQRFEATEAFAG
                     DIVAITGLGELNISDTICDINAVEALPALSVDEPTVTMFFCVNTSPFCGQEGKFVTSR
                     QILERLNKELVHNVALRVEETSNPDEFRVSGRGELHLSVLIENMRREGYELAVSRPKV
                     IYREEDGKKQEPFEQVTIDIEEQHQGSVMEALGIRKGEVRDMIPDGKGRTRLEYVIPS
                     RGLIGFRNEFMTMTSGTGLLYSTFSHYDDVKPGEIGQRKNGVLISNATGKALAYALFG
                     LQERGKLMIDHGVDVYEGQIIGIHSRSNDLTVNCLQGKKLTNMRASGKDDAIVLTTPV
                     RFTLEQALEFIDDDELVEVTPQSVRIRKRLLTENDRKRASRTTTSTSTH"
     gene            complement(5161..5838)
                     /locus_tag="PXD66_001707"
     CDS             complement(5161..5838)
                     /locus_tag="PXD66_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDL6319989.1"
                     /translation="MSIAENLSQIHQQIQQISEQYQRDNVRLLAVSKTKPVEAIQAAI
                     EAGQRAFGENYVQEAVEKIEFFANRPELEWHFIGPLQSNKTKLVAAYFDWIQTVDRLK
                     IAERLNEQRPADKAPLNVLIQINISDEASKSGIEPDEMLPLAQAISQLPNLKLRGLMA
                     IPKPESEPEQQKVAFRKMQLLFNRLQTEFDDIDTLSMGMSDDMQAAIECGSTMVRIGT
                     AIFGARR"
     gene            5909..6448
                     /locus_tag="PXD66_001708"
     CDS             5909..6448
                     /locus_tag="PXD66_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HDL6319990.1"
                     /translation="MLDLTKIKLVITDVDGVLTDGGMYYTDQGEVMKRFHVHDGLGVK
                     MLQSCGIKVAVLSGGDTALLRKRLEVLKIDLALLGKMEKRSACFELMQQAGVTPEQTA
                     YIGDDTLDLPAFEVCGIAIATRNALDYIKEKADWVLQKAGGEGAFREVSDQILEAQGF
                     GEIFKTADGFLTVAGKMAQ"
BASE COUNT         1808 a         1425 c         1321 g         1909 t
ORIGIN      
        1 tataaagctg ttgtgcttga tgattgtttt tatctgttac ccaatatact cggttatatc
       61 ctttttcctg tgcatatcga taaatatatt caattaattc ttttcctatg ccttggttgc
      121 gatattcagc taaaacaaat aaatcttcca aataacagca ttcagttaaa ttccaagtat
      181 ttgggtgaat cacaatatga acaaaaccaa ccattctgtt atcaacaaaa gccccaaatc
      241 cctttacatt ttcactctct gaaactttct gccaagttga ttgaataata tcatctgcta
      301 aattcacttg ataaaaatct aaatattgtt gccacaatag tttccattca gtgagatttt
      361 tggaggctaa cggcgaaata ttaatactca tattttttcc tgttatgctt ttaataaaat
      421 aaagtgttca atcagtttaa tcatcagttc tttttgttca ggcaaacttt ctgccaccaa
      481 caaggctaag acaaccagtg tattgactga gcaaaccaat cacctgctca aactcgatac
      541 ctcgttcctg caaacggcgt tcgttaaggg catagccttt caccagatag tcttttaggc
      601 gaacgacttc tctagcacct tcaatttgaa ctgttgcatt ttttgcaaca gttgcctcac
      661 gctccagttc accttcttgg tagatatttc gtaagtgccg agaaataaca gacttatctc
      721 gtccaaaaat atctgccatt tgctgtaaac ttagccacac cgtttcctta tcaaactgca
      781 cggaaacttg ggttttgccg tcttggcttt ggtaaatttc aatcgggttt ttcatattat
      841 tcccaaattt tatctataaa aaaatctctc gaaagttgcc ttccgagaga tctttattga
      901 tttgctacac ttgctcggaa gagttatctt ccgcacacac caaatgcccg aaagatatca
      961 ggttaaagtg tggtggtgat gtaaagtgta gtttaaattc attgcattac tccaaaacag
     1021 tatgaacaga atttacgtca agccggcaga aatatcaagc acttttttaa gcaaataccg
     1081 gcttttttat aaccgtttgt tctgcctatt atgttcttgc ggaatacacc ttaaacttgg
     1141 tggattttgc cagtacgtcg tgtttgccga aggctttatc gagcaaatct gggtaaggca
     1201 agaatgcgtt tgcgacaatt cgcagctcac cgcctttggt taggtgattt ttagcttgga
     1261 aaatcaactc ctccaccgct cggtaggcgg tatcgacccc gtcgtggaat ggtgggttgg
     1321 agacaattaa atcaaaccgt tcattaatgt gagagaacac atcacttgct accacttccc
     1381 cctctaactg attttccgcc aaagtacgtc tgccggattc caacgccatt gcgtgaatat
     1441 cgctcatcgt cagtttgatt ttcggaaatt gttgtttgag cgttgcccca atcacgccag
     1501 ccccacagcc taaatctaac actttgcctt ttagcctgtc tgctttgtcg aaggttgaaa
     1561 gcaataattt ggtgccgtta tccagctccg ccgagctaaa aaccgctggt agatcgaaca
     1621 catctaagtc ttgcaagcgg taggatttcc agaatttttt gcaatcgaat gtcggcacgg
     1681 tctgtagctc aaaatggtat aaaccacaac ggcgtgcaga gtcgattttg gcaatattgc
     1741 cgaacggttc gagcagtttt tctaccgaac gaacaccagc tcggttttcg ccgataatca
     1801 acatttcttg ccctgcttgg cattgcgaca gccattgtag cagttggaat tggcattcct
     1861 gcttgttctt cgtccaataa aacaccgcta actcggcact caaaccacat tctaagccaa
     1921 attcaacctc actatggtgg cgtgcgtaat caaaatagct actgaatacc gcaacctttt
     1981 ccgcacgaga ttgcagctgg cgggcaaaat catctcgcac atcgccaaat aataacaccg
     2041 acttgttttc aaataacgcc aaatggcgtt caagtacttc gctttctaaa gagagcattt
     2101 tcttacctta tctaaaaaat ttagcgtgaa tataccgaat ttaagtacag aaatcttgaa
     2161 ttttttgaaa aaagtttctg ttcaagcgga gttttgatac tatttgcaaa atttgatgag
     2221 gttttaaatg aatcgtcgtg atttactgtt acaggaaatg ggtatttcgc aatgggtgct
     2281 aaccaatccc caagtgctaa aaggcgatgc tcaaatccgc ctaaacaatg cggtaaaatt
     2341 ggtggtagtg tgtgaggaag atcaccaaca cagccggctc tttgccgata ttctgcgtgc
     2401 tttaaagtta aaaaacagcg aatatcaatg gctgactggc gaacaatcac aacgtttggt
     2461 gtttgagcat tcgccgatct tttggctgat tcaagcggaa gaacaagcgg tcaaaatggc
     2521 aaaaagtttt actaatcaga ccgcttggaa aaacgagtct tggcaagatt tagccaaatc
     2581 tacgaccaaa cgccaacttt ggcaacaaat tgatactttt tcggcacaat ttggagaaca
     2641 gcgtgattga gcaaattcaa gcggaagatt tcgagcagtt atttgaaatt gagcaacaag
     2701 cccatttagt gccgtggtca aaggggacgt tattaaataa tcagggtgaa aaatacctga
     2761 atttaaaagt ggtagagcaa aacaaaattg tggcttttgc cattagtcat atcgtgctag
     2821 atgaagcaac cttatttaac attgcagttg ctcctgattt tcaaggcaaa ggcattggca
     2881 agcggttact ttccgaatta attttgcaac tgcaacaacg gaaactcacc accctgtggt
     2941 tggaagtgcg agaatccaac cattctgcca gaaaactcta cgattcactc ggttttaatg
     3001 aagttaccac ccgcaaaaac tattacccca cgccaaacgg tagcaaagaa aatgcggtgg
     3061 taatggcgtt atatttgtag ggagaattta aaaccaaaag agcgacctca gtcgctcttt
     3121 tttatcagtt agtgcgtgct ggtactggtt gtagtacggc ttgcacgttt ccggtcgttt
     3181 tcagttaata aacgtttacg aatacgcacc gattgtggtg ttacttccac taattcgtca
     3241 tcgtcaataa actctaacgc ttgttctaga gtgaaacgca ccggtgtggt taaaacgatg
     3301 gcatcgtctt tacctgatgc acgcatattg gttaatttct taccctgtaa gcagtttacg
     3361 gttaaatcgt ttgaacggct gtgaatcccg ataatttgcc cttcatatac atcaacaccg
     3421 tggtcgatca ttaattttcc acgctcttgt aagccaaata atgcgtatgc taatgcctta
     3481 cccgttgcgt tagaaattaa cacaccgttt ttacgctgac cgatttcgcc cggttttacg
     3541 tcatcatagt ggctgaatgt agagtaaagt aaaccagtac ctgaggtcat cgtcataaat
     3601 tcgttacgga aaccgattaa gccacggctt gggatcacat actctaaacg ggtacgacct
     3661 ttgccgtccg gaatcatatc acgcacttca cctttacgga taccaagtgc ttccatcact
     3721 gaaccttggt gttgctcttc gatatcaatc gtcacttgct caaacggctc ttgttttttg
     3781 ccgtcttctt cgcggtagat tactttcggg cgagacactg ctaactcgta accttcacga
     3841 cgcatatttt cgattaatac cgataagtgt aattcgccac gacctgacac acggaattcg
     3901 tctgggttcg aagtttcttc cacacgcaat gccacgttgt ggactaattc tttgtttaaa
     3961 cgctcaagaa tttggcgtga agtcacaaat ttaccttctt gaccgcagaa tggcgaggtg
     4021 tttacgcaga agaacatcgt taccgttggt tcatcaacgc ttaatgctgg taaagcctct
     4081 actgcgttaa tatcacaaat agtatctgaa atatttaatt cgcctaaacc agtaatcgca
     4141 acaatatcgc cagcaaaggc ttctgttgct tcaaaacgtt gtaaacctaa atgccccaac
     4201 acctgaccga tacgaccttg gcgagttttg ccttcgctat ctacaatcgt taccgcttgg
     4261 ttaggtttca ccgaaccacg tttgatacgt ccaataccga taacgcccac atagttgttg
     4321 tagtctaatt gcgaaatctg catttggaat ggtgcatcta attccacttg tggtggttga
     4381 acgtgtttga caatcgcttc ataaagtggg gtcatatctg ccgctaggtc ttcgtgttct
     4441 aagcctgcta caccatttaa ggctgaagcg tagataattg ggaaatctaa ctgctcatca
     4501 cttgcaccta aatttacaaa caagtcgaac acttgatcca ccacccaatc agggcgagca
     4561 cctgggcggt caactttatt gatcactacg attggtttta aaccgtgagc gaacgctttt
     4621 tgcgtgacga aacgggtttg cggcatcggg ccatcaaagg catcaaccac taacagaaca
     4681 gaatctacca ttgaaagtac acgctcaacc tcaccaccga agtcagcgtg ccctggggta
     4741 tctacgatat tgatgtgata accattccag ttaattgccg tattttttgc aagaatggta
     4801 atgccacgct ctttttcaag atcgttggaa tccattacac gctcatcaac gtcaccacga
     4861 gtttcaccaa aggtgcctga ttgttgtaaa agtttatcaa ccaaggtggt tttaccgtgg
     4921 tcaacgtggg caataattgc aatgttacgc aattttgaaa tatctacatt ttgcattgga
     4981 atatctttaa gttaagtcaa aataaaaact ctcgcccatc aaaactggcg agagtcaaga
     5041 aattaggagg cgtaattata cagacttttt ccgctgttgg ctacaaaaca agcggtggaa
     5101 tttgcaaagt ttttggcgaa tttgaccgct tgtatcagct ttatgtaaaa actcagctgg
     5161 ctaacgtctc gccccaaaaa tcgcagtccc aatccgcacc attgtcgagc cacattcaat
     5221 cgccgcttgc atatcgtccg acatccccat tgaaagggta tcaatgtcgt caaattctgt
     5281 ttgcaagcgg ttaaaaagta gctgcatttt gcgaaaggca actttttgtt gctctggctc
     5341 gctttctggt ttgggaatgg ccattaagcc ccgcagtttc aaattcggta attgagaaat
     5401 cgcttgggca agtggtagca tttcatctgg ttcaatgccc gatttcgagg cttcatcgct
     5461 gatattaatt tgaatcagca cgttgagcgg ggctttatcc gctgggcgtt gttcgtttaa
     5521 acgctcggca atttttaatc tatccaccgt ttgaatccaa tcaaaatagg cagcgaccaa
     5581 tttggtttta tttgattgaa gtggcccaat aaaatgccat tctaattcgg ggcgatttgc
     5641 aaaaaattcg attttttcga ccgcttcctg cacataattt tcgccaaatg cacgctgccc
     5701 tgcctcaatc gccgcttgaa tcgcctcaac tggtttagtt ttggagacgg ctaacaaacg
     5761 cacgttatct cgttgatatt gttcggaaat ttgctgaatt tgttgatgga tttgcgataa
     5821 attctcggca atcgacattt cggttccttt ggtaaacgga ggtaaaaaag ctataatggt
     5881 aaaatcatac catatttgag gcaaaaaaat gttagatctc accaagatta aattagtgat
     5941 taccgatgtg gacggcgtgc taaccgatgg cggaatgtac tacaccgacc aaggcgaagt
     6001 aatgaaacgt ttccacgttc acgatggttt aggcgttaag atgttacaaa gttgtggcat
     6061 taaagtggca gtactctcgg gcggcgatac ggctttgctc cgcaaacggt tagaggtgct
     6121 gaaaattgat ctcgctttgt taggcaaaat ggaaaaacgc tccgcctgct ttgagctaat
     6181 gcagcaagcc ggtgttaccc ctgagcaaac ggcttacatt ggtgatgaca ccttagattt
     6241 accggctttt gaagtgtgtg gcattgcgat tgcaacccgt aacgccctcg attacatcaa
     6301 agaaaaagca gattgggtat tgcaaaaagc cggtggtgaa ggggcgtttc gtgaagtctc
     6361 cgaccaaatt ttggaggctc aaggattcgg tgaaattttt aaaaccgcag atggtttttt
     6421 aacggtagcg gggaaaatgg ctcagtagac tcttcaagca gtt
//
LOCUS       DANLXF010000133         6457 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.133, whole genome shotgun sequence.
ACCESSION   DANLXF010000133 DANLXF010000000
VERSION     DANLXF010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6457
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.133"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(5..916)
                     /locus_tag="PXD66_001709"
     CDS             complement(5..916)
                     /locus_tag="PXD66_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HDL6319991.1"
                     /translation="MKWLMLCREPRLYSCCRIKAACEAKGIELDILDQNRMLLGLENG
                     EFKIYYQAGESYDNNRPEPVLLPNYDGILPRFGTGSTEMGCSVLRHFEAKGIPVLNHS
                     AAFALARDKWRTLQKLAKHNLPVPNTNFAGHLVSVKSQLNQFAFPLISKVLNGSQGNG
                     VMLFEGKNNAEAVLATFRQVNEPYLCQQFVGDAKGQDIRAFVIGNEVVAAMSRTSVTG
                     DFRANIHQGGTAQPIELTQMERALAIKATKTIGLDIAGVDFLRTEKGVVILEVNASPG
                     FEGIERVNDVDIASEMVSYFINKMKNA"
     gene            complement(913..1632)
                     /gene="nfsA"
                     /locus_tag="PXD66_001710"
     CDS             complement(913..1632)
                     /gene="nfsA"
                     /locus_tag="PXD66_001710"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NADPH nitroreductase"
                     /protein_id="HDL6319992.1"
                     /translation="MQSKPTLETILSHRSIRKFTEQPISEEIFQILIEAGKAASSSNH
                     LQCVTVIRVTDRQIRETLKAVSGMQYVVDCAEFLVFCIDFNKHKQLVPESQLEWAEVS
                     LIGAVDTGILAQNVFLAAESLGLGAVYIGALRNDIERVSKALNLPEYCVPLVGMCLGY
                     PAQEPAIKPRLPNSLVCYNNQYQDFDWTEFAKYNENLTAYYQARIGEPQQWQAAIHKT
                     LDKPVRPHILPFFNQKGLLKK"
     gene            complement(1644..2429)
                     /gene="mtgA"
                     /locus_tag="PXD66_001711"
     CDS             complement(1644..2429)
                     /gene="mtgA"
                     /locus_tag="PXD66_001711"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HDL6319993.1"
                     /translation="MEQSMAKRNKRFLSSLLKKIGLFFIPRKIKSKLGWLWFGSSRLL
                     TFGGTFFLIPILIFSVLPVPYSAYMVQKKVENLVQGKSYPIKKEWVSLNEISWQMQMA
                     VIASEDQKFESHFGIDLQAIETALKLNAKSKKVRGGSTISQQTVKNMFLWHGQSWLRK
                     GIEVPLTLLVENVWSKDRILEVYLNIAEFGNGIFGVEAAAKHFFKKSAKQLTLQESAL
                     LAASLPNPLVFRVDKPGPSMRKRQAWIMRQVSLLDGKNYLDKL"
     gene            complement(2404..2724)
                     /gene="trpR"
                     /locus_tag="PXD66_001712"
     CDS             complement(2404..2724)
                     /gene="trpR"
                     /locus_tag="PXD66_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HDL6319994.1"
                     /translation="MKTLYNQRDPNEWTQFLALLEQAVAEDKLEPFFSMFLTADERGS
                     LGLRLQIVQALLKGEDSQREIQQNLNTSAATITRGSNMLKTLDQDFLQWLNGKVNGTV
                     NGKA"
     gene            complement(2804..3445)
                     /gene="lolA"
                     /locus_tag="PXD66_001713"
     CDS             complement(2804..3445)
                     /gene="lolA"
                     /locus_tag="PXD66_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HDL6319995.1"
                     /translation="MKTLFKKSVFGLALFGLANVALADAQSVAELQRRLEQVSQYSAD
                     FDQTVRSSKGKQIQQGKGKFQVKRPNLFRMDTKSPQENLIVSDGKDLWFYDPFVSQVT
                     VNTVQDAVNNTPFVLLTSGDKSHWDQYDVSQSADTFVLKPKSKKSNLKQFDVRIDQSG
                     LLKGFSTIERDGQSNLYVLRNITTGGVSSDLFKFSVPKGAELDDQRGGKKSKK"
     gene            complement(3556..6414)
                     /gene="polA"
                     /locus_tag="PXD66_001714"
     CDS             complement(3556..6414)
                     /gene="polA"
                     /locus_tag="PXD66_001714"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDL6319996.1"
                     /translation="MATIAKNPLVLVDGSSYLYRAFHAFPPLTNRLGEPTGAMYGVLN
                     MLKSLISQVNPSHIAVVFDAKGKTFRDELFKQYKSHRPPMPDDLRPQIEPLHRIIKAL
                     GIPLISIEGVEADDVIGTLAVQAAKDGKDVLISTGDKDMAQLVNDHIMLINTMNNTLL
                     DREGVIEKYGIPPELIIDFLALMGDSADNIPGVKGVGEKTALALLQGIGSLEQIYANL
                     DQIATLSFRGAKNFAPKLEAEKANADLSYLLATIKTDVELDVTHDQLLTQPQDRAELA
                     QLFEHYEFKRWLNEVNQNANPVTQTSAEKVPNNYQVTQAVRNEENVANVVEIDRSKYE
                     TVDTEQKLSAWIEKLTAARLIAVDTETDSLDAMSANLVGISFALENGEACYIPLGHKQ
                     KAQPQQADMFGDEERSESPSSDELVKNQLNLTACLSKLKPILLNPEIKKIGQNIKYDL
                     TIFANYGIELQGVAFDTMIESYTQNSTGRHNMDDLAARYLGHKTIPFEELAGKGKNQL
                     TFDKIEIAKAAEYAAEDADVTMKLHQVLWEKLQQEPELVKLFDEIEMPLVGVLSRIER
                     NGVLIDPKKLLTQSAEIEKRLEEVETLVHAEAGQTFNLASTKQLQEILFEKLGLPVLK
                     KTPKGAPSTNEEVLDELAQQGHLVPKLLMEHRGLSKLKSTYTDKLPQLINKKTGRVHT
                     SYNQAVTATGRLSSSDPNLQNIPIRNEEGRRIRQAFVAREGYKIVAADYSQIELRIMA
                     HLSNDEGMINAFAEGKDIHRATAAEIFGVELGEVTSEQRRSAKAINFGLIYGMSSFGL
                     SNQLGIGRAEAQKYMDLYFQRYPAVQQFMTDIREVAVEKGYVETLFGRRLYLPDIKSG
                     NAILRKAAERVAINAPMQGTAADIIKVAMIGIDNAIRDNDEIKMIMQVHDELVFEVKA
                     DKIDHYSQLIKTEMEKAISLKVPLIAEVGVGENWDEAH"
BASE COUNT         1759 a         1504 c         1219 g         1975 t
ORIGIN      
        1 caaatcaagc attcttcatc ttattgataa aataagaaac catttcagaa gctatatcaa
       61 catcatttac tctctctatg ccttcaaatc ctgggctggc attgacttct aaaataacta
      121 cgcctttctc tgtccgcaga aaatcaacgc ctgcaatatc aagccctatc gttttagttg
      181 ctttaattgc taacgcacgt tccatctgag ttaattcaat cggttgagcc gttccacctt
      241 gatgaatatt ggctctaaaa tcgcctgtta cgctggttcg gctcatggca gcaaccactt
      301 cattgccaat cacaaacgcc cgaatatctt gccctttagc gtcgcccaca aattgttggc
      361 atagataagg ctcatttact tgcctaaagg tcgctaacac ggcctcggca ttatttttgc
      421 cttcaaatag cattacgcca ttcccctgtg aaccatttag cacttttgaa ataagtggaa
      481 aagcaaattg attgagttgt gatttcacag aaaccaaatg ccctgcaaaa ttcgtattag
      541 gaaccggcaa attgtgtttg gctaattttt gtaatgttcg ccatttgtct cgggctaacg
      601 caaatgccgc tgaatgattt agaacaggaa tacctttcgc ctcaaaatgg cgtaacaccg
      661 aacagcccat ttcggtactg cctgtgccaa aacggggtaa aatgccgtca taattcggca
      721 acaataccgg ctccggacgg ttgttatcat agctctcgcc agcttgatag tagatcttaa
      781 attcgccatt ttctaacccg agtagcattc tattctggtc taaaatatcc agctcaatcc
      841 cctttgcctc acaagcggct tttattcgac agcagctata taaacgaggc tctcgacata
      901 gcattagcca cttcattttt tcagcaatcc tttctgattg aaaaacggca aaatatgcgg
      961 acgaacaggc ttatccaatg ttttatgaat agctgcttgc cattgttgtg gctcgccgat
     1021 tctggcttgg tagtaagcgg tcaaattttc attatatttt gcaaattcag tccaatcaaa
     1081 atcctgatat tgattgttat aacaaaccaa actattcggc aaacgaggtt ttattgccgg
     1141 ctcttgtgcc ggatacccca aacacatacc caccagtgga acacaatatt ccggcaagtt
     1201 aagggcttta gaaacccgct caatatcatt acgcaatgct ccaatataca ccgctcccag
     1261 ccctagtgat tcggcggcta aaaatacatt ctgggccaaa ataccggtat ctaccgcccc
     1321 aatcaacgac acctccgccc attccaactg cgattcaggg acaagttgtt tgtgtttatt
     1381 aaaatcaatg cagaacacca aaaattcagc acaatctact acatattgca tacctgaaac
     1441 ggcttttaat gtttctcgaa tctgccgatc ggttacacga atcaccgtca cacattgcag
     1501 atgatttgaa cttgaagccg ctttacctgc ctcaattaag atttggaaaa tctcttcaga
     1561 aataggctgt tcggtaaatt tacgaataga acggtgagat aaaatcgtct caagagtcgg
     1621 tttactttgc atatttttct cgtctaaagt ttatctaaat aatttttgcc atcaagcaaa
     1681 cttacttgtc gcataatcca cgcttggcgt tttcgcatac tcgggccggg tttatctacc
     1741 ctaaacacca acgggttagg caaagaggct gccaatagtg cggattcttg caaggttagc
     1801 tgtttggctg attttttgaa aaaatgcttt gcggctgctt ccaccccgaa aatgccgtta
     1861 ccaaattccg caatattgag gtacacctct aaaatccgat ccttactcca cacattttcg
     1921 actaacaggg ttaaaggcac ttcaatccct ttgcgtaacc agctttgccc gtgccacaaa
     1981 aacatatttt ttaccgtttg ttgcgaaatg gttgaacctc cacgcacttt tttggattta
     2041 gcattcagtt tcaaggcagt ttctatcgct tgtaagtcaa tgccaaaatg gctttcaaac
     2101 ttttgatctt ctgaggcaat cacagccatt tgcatttgcc acgaaatctc attcaggctc
     2161 acccattctt ttttgatcgg gtaagatttc ccctgaacca gattctccac ctttttttgc
     2221 accatatacg cagaataagg caccggtaac accgaaaaaa tcagtatcgg aatcaggaaa
     2281 aatgtgccac caaaggttaa taagcgacta gaaccaaacc aaagccagcc cagtttactt
     2341 tttattttgc gaggaataaa aaaaagccct attttcttca aaagagagct taaaaatcgc
     2401 ttgttacgct ttgccattga ctgttccatt aaccttgccg ttcagccatt gcagaaaatc
     2461 ttggtcgagt gttttaagca tatttgaacc acgagtgatg gttgccgcac tggtatttaa
     2521 attttgttgg atttcccgtt gcgaatcttc accttttagc aaagcttgca caatttgtaa
     2581 acgcaagcct aatgaacctc gctcatcggc ggtgagaaac attgagaaaa acggctcaag
     2641 tttatcttcc gcaaccgctt gttcaagcag agccaaaaac tgcgtccact cgtttggatc
     2701 tcgctggtta tagagtgttt tcatactgtc ccttgaagta aaaacggtga caagcggtca
     2761 gatttttaca aaattttgca gaaaaacgac cgcttgctct ccattatttt ttcgattttt
     2821 taccgccacg ttggtcgtct aactctgccc ctttcggtac gctgaattta aacaaatcgc
     2881 ttgagacacc gccggtagta atattgcgta acacatataa gttactttgc ccgtcacgct
     2941 caatggtact gaaacctttt aataaaccgc tttggtcaat ccgcacatca aactgtttta
     3001 aattgctttt tttcgatttt ggcttaagca cgaaagtatc agcactctgg ctgacatcat
     3061 attgatccca gtggctttta tcaccgctgg ttaataagac gaatggcgta ttattaactg
     3121 catcttgcac ggtatttacc gtaacttgtg aaacgaaagg atcgtaaaac cataaatctt
     3181 tgccgtcaga tacaatcaaa ttttcttgtg gtgatttggt atccattcta aataagttcg
     3241 ggcgttttac ttggaatttg cctttgcctt gctgaatttg tttacctttg ctagaacgca
     3301 cggtttggtc gaaatccgca ctatattggc ttacttgttc caaacggcgt tgtaattctg
     3361 caacagattg agcatcagcc aatgccacat ttgccaagcc aaataacgct aaaccgaata
     3421 ccgatttttt aaataatgtt ttcatttgtt ttccttttta ccaacaagaa ttttcttgtt
     3481 tgacaataaa atcatcaaaa gtttcatatt aatgaactgt tacaacaata caataaaagt
     3541 cgtgaattgt atagtttaat gagcttcgtc ccagttttcg cctacgccaa cttcggcaat
     3601 cagtggcact ttgaggctaa tcgctttttc catttcggtt ttgattaatt ggctgtaatg
     3661 gtcgatttta tcggctttga cttcaaacac caattcatcg tgaacctgca taatcatttt
     3721 gatttcatca ttatcacgaa tcgcattatc aataccaatc atcgccacct taataatatc
     3781 ggcagccgtg ccttgcattg gggcgttgat tgccacccgt tctgctgctt tacgcaaaat
     3841 agcattgccg gatttaatat caggcaagta caaacggcga ccaaataggg tttcaacata
     3901 gcctttttca actgcaactt cacgaatatc ggtcataaat tgttgaaccg ccgggtaacg
     3961 ttggaagtag agatccatat atttttgagc ctctgcacgc ccaatcccta attggtttga
     4021 aagcccaaat gacgacattc cgtaaatcag cccgaagtta attgctttcg cactgcggcg
     4081 ttgttcggag gtgacttcac ctaactccac cccgaaaatt tcggcagcgg tagcacggtg
     4141 aatatctttg ccctccgcaa aggcgttaat cattccctca tcattcgata aatgagccat
     4201 aatccgtagc tcaatttgtg aatagtcggc agcgacaatt ttataacctt ctctcgcgac
     4261 aaaagcttgg cgaatccgtc tgccctcttc attgcggatc ggaatgttct gcaaattcgg
     4321 atcgctggaa gaaaggcgtc cggttgcggt gactgcttga ttgtaagagg tatgcacccg
     4381 ccctgttttt ttgttaatca gttgtggtaa tttatcggtg taggtagatt tcaacttact
     4441 taacccacga tgctccatta ataacttcgg cactaaatgc ccttgttgag ccaactcgtc
     4501 taacacttct tcgttggttg agggtgcacc ttttggagtt ttcttcaaca ccggcaagcc
     4561 taatttttca aacaaaatct cttgtaattg tttagttgag gcaagattga aggtttgccc
     4621 tgcttctgcg tgtactaatg tttcaacttc ctccaaacgt ttttcaattt cagccgattg
     4681 ggtcaataat tttttcgggt caatcaacac gccattgcgt tcaattcgag aaagtacgcc
     4741 cacaagtggc atttcaattt catcgaataa cttcaccaat tcaggctctt gctgtagttt
     4801 ttcccacaat acttggtgga gtttcatcgt cacatcggca tcttctgccg catattcagc
     4861 cgcttttgct atctcgattt tatcgaaggt cagttgattt ttccccttac cagcaagctc
     4921 ttcaaacgga atcgttttat gccccaaata acgggcggct aaatcgtcca tattatgacg
     4981 accggtgctg ttttgggtgt aagattcaat catcgtatca aaagcaacac cttgtagctc
     5041 aatgccgtag ttcgcaaaaa tggttaaatc atatttaata ttctgcccga tctttttaat
     5101 ctccgggtta agcaaaatcg gcttgagttt acttaaacaa gcggtcaaat ttagctgatt
     5161 ttttaccaat tcatcggaag acgggctttc tgaacgctcc tcatcaccga acatatcggc
     5221 ttgttgcggt tgggcttttt gcttatgccc aagcggaata tagcacgcct cgccattttc
     5281 cagtgcaaag gaaataccga ccaaattagc cgacattgcg tcaaggctgt cggtttcggt
     5341 atccaccgca atcaaacgag ctgcggtgag tttttcgatc caggctgata atttctgttc
     5401 agtatcaacg gtctcatatt tgctacgatc gatctcgacc acatttgcaa cattttcctc
     5461 atttctgacc gcttgtgtta cttggtaatt attcggtact ttttcagccg aagtttgagt
     5521 gaccggattg gcattttgat tcacttcgtt cagccagcgt ttaaattcat aatgctcaaa
     5581 taattgagca agctcagcac ggtcttgcgg ctgtgttaaa agttgatcgt gagtaacatc
     5641 aagttccaca tcggttttga tggtagcaag caaataggat aaatcggcat tcgctttttc
     5701 cgcttccaat tttggggcga aattttttgc cccacggaaa gaaagggtgg caatttgatc
     5761 caaattagca taaatctgtt caagtgaacc aatgccttgc aataaagcta atgcggtctt
     5821 ttcccccaca ccttttacgc ccggaatgtt atcggcggaa tcgcccatta atgccagaaa
     5881 gtcgataatc aactcaggcg gaatgccata tttttcaatc acgccttcac gatctaacaa
     5941 agtattgttc atcgtgttaa tcaacataat gtgatcgttc accaattgtg ccatatcttt
     6001 atcgccggtg ctgatcaaca catctttgcc gtctttagcg gcttgcacag caagcgtgcc
     6061 gatcacatca tcagcttcca caccttcaat cgaaatcagt ggaatgccta aggctttgat
     6121 aatgcgatgc aaaggctcaa tttgagggcg taaatcatcg ggcattggtg ggcggtgcga
     6181 tttatattgc ttaaacaact catcacggaa ggttttccct ttggcatcaa acaccacagc
     6241 aatatggctt ggattcactt gtgaaatcag gctttttaac atattcagca cgccatacat
     6301 tgcccctgtc ggttcgccta agcgattagt gagcggaggg aaagcgtgaa atgctcgata
     6361 taaataggaa gaaccatcca ccaataccag tggatttttt gcgatagttg ccataaaaat
     6421 ctctttataa tcaaataaat attggcgtta ttataca
//
LOCUS       DANLXF010000134         6440 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.134, whole genome shotgun sequence.
ACCESSION   DANLXF010000134 DANLXF010000000
VERSION     DANLXF010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6440
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.134"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            121..1038
                     /locus_tag="PXD66_001715"
                     /pseudo
     CDS             121..1038
                     /locus_tag="PXD66_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167835.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(1040..1708)
                     /locus_tag="PXD66_001716"
     CDS             complement(1040..1708)
                     /locus_tag="PXD66_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HDL6319997.1"
                     /translation="MKYLYALIFSLFFSSVYAAERQLQCKVVGISDGDTLTCLVNKTP
                     LKIRLQHIDAPEQAQPYGNKAKQALAQFVFQQNITAIVSGYDRYQRLLATLYLNDKNI
                     NLALVENGMAWAYTTKREYIEAQDQAQQAKIGLWQDKFPINPSQWRKTHTSTLPQAVK
                     KPENIANQPPTIDCQIKLSCNQIGNYAQAKRYFQQCGWKELDGNNDSIPCNKLYRQEQ
                     KNRN"
     gene            complement(1752..3119)
                     /locus_tag="PXD66_001717"
     CDS             complement(1752..3119)
                     /locus_tag="PXD66_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HDL6319998.1"
                     /translation="MSIDTILNQINSFVWGPPLLILISGVGLYLTFSLKGIQLSQLGQ
                     AFRYMFKPEKGKGQSGDISAFAALSTALAATIGTGNIVGVATAIHSGGPGALFWMWLI
                     ALFGMATKYAEGLLAIKYRTKDRAGFVAGGPMYYIELGMGKKWRWLAKAFAFFGVLVA
                     LLGIGTFPQVNGITHALESTFDVPIIFSAIILTLIVTAIILGGVQRISKIATIVVPFM
                     AIGYVIAALSVILLNMEKVPQAIITIVHAAFNPQAALGGAVGFTVMQAIQLGVARGIF
                     SNESGLGSAPIAAAAAQTKEPVRQGLISMTGTFLDTIIVCTMTGLVIVISGAWAGDVK
                     GAELTTTAFTQGLNSSFGAIIVTVGLVFFAFTTILGWCYYGERCFVYLTRGRTQYIKL
                     YRLLFIALIASAPFLQLDTIWTLADIVNGLMAFPNLIALIALRKVIISETKDFFARLK
                     TGKAE"
     regulatory      complement(3238..3333)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(3504..4556)
                     /locus_tag="PXD66_001718"
     CDS             complement(3504..4556)
                     /locus_tag="PXD66_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL6319999.1"
                     /translation="MTHSTKTLVAIASIIIILAGIKLSNEIVVPFLLSLFIAIICSPM
                     IKFMTKRKVPLWLAITILLIVFILGFSFLAGIINNTLQEFTNAIPQYKIQLSSRIQVI
                     LELAHRWHIPLTLSQEEIMSKFDPSSIMNLVSKLLMGLSGALSNIFVIFLVVIFMLLE
                     MPVAKYKLALALSPDDDLSNEKEYINQVLEGVIGYLGVKTFVSLLTGGLVWILLEILG
                     VEYAILWAILTFLLNYVPNIGSILAGIPIVIQALVLNGFSIGLGTLIGIVVINMVVGN
                     ALEPKLMGKKLGLSTLVVFLSLLFWGWLLGTVGMLLSVPLTMACKVALESNKATQKYA
                     VLFSDLEEVPKEQKYR"
     gene            complement(4603..5028)
                     /gene="ruvX"
                     /locus_tag="PXD66_001719"
     CDS             complement(4603..5028)
                     /gene="ruvX"
                     /locus_tag="PXD66_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDL6320000.1"
                     /translation="MTETTTKTVIAFDFGTNSIGCAVGQSITGTAQGLTTFKAQDGIP
                     NWEEIGKLLQDWKPDMLVVGLPLNMDGTEQPLTQRARKFANRLNGRFNLPVELQDERL
                     TTVEAKAEIFSRGGYKALKKDKVDSISACLILESWFENH"
     gene            complement(5028..5588)
                     /locus_tag="PXD66_001720"
     CDS             complement(5028..5588)
                     /locus_tag="PXD66_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HDL6320001.1"
                     /translation="MLENLQGKFLIATPEMDDDYFERTVVYICEHNPQGAMGVVINTP
                     TDLSVLELITRMDFQMANQRDYKKDQMVLSGGPVNQDRGFIIHTKTANDFIHTYPITD
                     KITLTTSGDVLDSFGTLASPEKFIVCLGCSTWRGSQLEQEIAQNYWIVSDANEKTLFE
                     TGYLERWAEAYEMMGLSSIPSRAGRA"
     gene            complement(5588..6319)
                     /gene="rsmE"
                     /locus_tag="PXD66_001721"
     CDS             complement(5588..6319)
                     /gene="rsmE"
                     /locus_tag="PXD66_001721"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007524366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDL6320002.1"
                     /translation="MRIPRIYQPQPLAGLQSCVLSEDAANHVGRVLRMKQGEQIILFD
                     GSNHVFHATLQAVEKKQIIAKIDSSELDDRESNLPIHLGQVISRGDRMEFTIQKSVEL
                     GVKTITPLWSERCGVKLNDERQDKKLQQWQKIAIAACEQCGRNEIPEIRPIMKLTDWC
                     KEQDDMLKLNLHPRAKYTIRELPNVPKAGVRLLIGSEGGLSAEEIAMTESEGFTEVLL
                     GKRVLRTETASLAAITALQVCFGDI"
BASE COUNT         1890 a         1291 c         1324 g         1935 t
ORIGIN      
        1 gaaactttat atggtattac aacgttaaaa tcattaggat tggaaaataa acgacaagaa
       61 cagtggatgt cgctcaatgc ggatgctttt aacacatcaa ttaaggtaac taaatttgat
      121 atgctgtttt ccggtattca tacctttatt tctaccatcg aacagatttt aattctttgg
      181 attggggcga caatgatgat tgaaaatgtg atgacattag gaatgtttgt cgcttttaat
      241 gcctatcgag gctctttctc ttcacgaatg gggaatttaa ttgatattat ttttagccta
      301 aaaattcttt cattacatag agagcggatt gcagacattg ctttaaatga agtcgaagat
      361 gaaagtaaat atcaagaact cagatttgaa cataaccaaa cagatataga agttaaagac
      421 ctttgtttca aatacgatcc tttttctaaa aatgtgcttg aaaatgtcaa tttagtgatc
      481 agaagtgggg aaagtgttgc tattaccgca ccatcaggtt atggcaaaac aacgttatta
      541 aaattaattg caggtttatt aaaaccaacc tcaggttctg tgtattttaa cggcattgat
      601 atttatcagt taggattaag tcaatatcgg agccaaattg cttgcgtatt acaggaagat
      661 aaattctttt ctggttccat tttagagaat attgttagtt ttgaaagtaa ttatgatcga
      721 gaatgggcta tcgaatgtag caaacttgcg aatattcaca atgatattat ggcaatgccg
      781 atgaattatg aaaccttatt aggagaatta ggaaataatt tgtctggcgg acaaagataa
      841 cgattgttta ttgctcgagc tttatataaa aagcctaaaa ttcttttgat ggatgaggcg
      901 actagccatt tagatgaaga aaatgagaag cttgtcaatc aagctatttc acgaatgaag
      961 ataacaagga ttattattgc tcatagacaa tcgactattc agtcggcgga tagaattatt
     1021 gccctgaatg acaggtaaat taattcctat ttttctgttc ttgccgataa agtttattgc
     1081 acggaatgct gtcgttgttg ccgtctagct ctttccagcc gcattgttga aagtagcgtt
     1141 ttgcctgtgc gtaattgccg atttggttgc agcttaattt aatttggcaa tcaatcgttg
     1201 gtggctggtt tgcaatattt tccggttttt tgaccgcttg tggtaaggtt gaggtgtgtg
     1261 tttttcgcca ttggctcggg ttaatcggga atttgtcctg ccataagccg attttagctt
     1321 gctgtgcttg atcttgtgct tcgatatatt cccgctttgt ggtgtaagcc cacgccatac
     1381 cgttttccac taaggctaag ttgatgtttt tatcgtttaa gtagagggtg gcaagtaatc
     1441 gttggtaacg atcatagcct gaaactatgg cagtaatgtt ttgttgaaac acgaattgag
     1501 ccagagcttg tttggctttg ttgccgtaag gctgcgcttg ttcgggggca tcaatatgtt
     1561 gtagccttat ttttagtggt gttttattga ccaaacaagt gagggtgtcg ccatcgctaa
     1621 tgccaacgac tttgcattgt agttggcgtt cggcggcata taccgatgag aaaaataagc
     1681 taaaaatcag ggcgtaaaga tatttcatta ttaaaaaaga gagggctaat aagccctcat
     1741 tctatcattt tttattcggc tttacctgtt ttaaggcgag cgaagaaatc ttttgtttca
     1801 ctaataatca ctttgcgtaa cgcaatcaat gcgattaaat tcgggaatgc cattaagccg
     1861 ttcacgatgt ctgccagcgt ccaaattgta tcgagttgta aaaatggagc agaggcgatg
     1921 agtgcaataa acagcaagcg gtataattta atgtattggg tgcgacctcg agtgagatag
     1981 acaaaacagc gttcaccgta gtagcaccag cctaaaatgg tggtaaaggc aaagaagact
     2041 aaaccaactg tcacaattat cgcaccgaat gagctgttta aaccttgagt aaaagcggtt
     2101 gtggtgagtt ctgcgccttt tacatcgcct gcccaagccc ctgaaatcac aattactaag
     2161 ccggtcattg tgcagacgat aatagtgtct aagaacgtgc cggtcattga aattaggcct
     2221 tgacgtaccg gctctttggt ttgggcggca gcggcggcga tgggggcaga gcctaatcct
     2281 gattcattcg agaaaatacc acgtgcaacc cctaattgaa tcgcttgcat gacggtgaat
     2341 ccgactgctc cgcctaatgc cgcttgtggg ttgaaagctg cgtgtacaat agtaataatt
     2401 gcttgtggga ctttttccat attcagtaga atgacggata aagcggcgat gacataccca
     2461 atagccataa acggtacaac aatggttgca attttagaaa tccgctgcac gccacctaaa
     2521 ataatagcgg taacgataag ggttaaaatg atcgcactga agataatcgg cacatcaaag
     2581 gtgctttcta aggcgtgggt aatgccattc acttggggga acgtgccaat accaagcaaa
     2641 gcgaccaata cgccaaaaaa cgcaaaggct ttagcaagcc aacgccattt ttttcccatt
     2701 cccagttcga tatagtacat cggaccacca gccacaaatc cggctctatc tttagttcga
     2761 tatttaattg ccaataagcc ttcggcatat ttcgtagcca taccgaataa ggcaatcagc
     2821 cacatccaaa ataatgctcc aggcccgccc gaatgaatag cggttgccac gccgacaata
     2881 ttccccgtgc cgatagtagc tgctagggca gtggaaaggg ctgcaaaagc ggaaatatcg
     2941 cccgattgcc ctttgccttt ttccggtttg aacatatagc gaaaggcttg ccctagttgg
     3001 cttaattgaa tacctttgag tgaaaaggtt aaatataacc ctacgcctga aattaaaatc
     3061 aggagtggcg ggccccaaac aaaactgttg atttgattaa gaatagtgtc gatagacatt
     3121 tgatgttcct cgtaaaaact gcttacaagg aaagaaaagg aatgcaagag acaagcggtc
     3181 aaaaaacgga aattttttgc aaagaattag caaaaaaaca ccgcttgtat cgtataactt
     3241 ttctcccctg tccttttgcc tgagcgtttc acacattgct gtattttcgc cttcggcggc
     3301 ggattttaat ccgctctctc caagggttcg tccagtaaca gtccccgcag tgttcactgc
     3361 acctgaaaga gtttacctcg tcggtgaaat gttaattcat ttctctccag ctaccttcat
     3421 ccgaacaatc taatttattt tataatgtaa aataaaaaag atttgccaaa ttctagcaac
     3481 tcttttgctt ccgtcaagtg aatttaacga tatttttgtt ctttcggtac ttcttctaaa
     3541 tcgctaaata atacggcata tttttgggtg gctttattgg attctaatgc cactttacac
     3601 gccatcgtaa gtggaacaga aagtaacatt cctacggtgc caagtaacca cccccaaaat
     3661 agaagtgaga ggaacactac aagggtagaa agcccgagtt tcttgcccat taatttaggt
     3721 tcaagcgcat tacccacaac catattaatc acgacaatgc cgattaaagt gcctaatcct
     3781 atagaaaagc cattgagtac caaggcttga atcacaattg gaattcccgc caaaatagag
     3841 ccgatattcg ggacgtagtt aagcaggaag gttagaattg cccataaaat cgcatattct
     3901 acgcctagaa tttctagtaa gatccaaact aaccctccgg tgagtaggct cacaaaggtt
     3961 ttaactccaa ggtaaccaat caccccttca agtacttggt tgatatactc tttttcgttg
     4021 cttaaatcat catctgggct aagtgcgagg gctaatttat atttggcaac cggcatttcc
     4081 aatagcataa aaatgacgac taagaaaatc acaaaaatat tcgataatgc accggatagc
     4141 cccattagca gtttgctgac caaattcatt attgagcttg ggtcaaattt gctcataatc
     4201 tcttcttgtg agagggtaag tggaatatgc catctatggg cgagttctag aatcacctgg
     4261 attctagatg aaagctgaat tttgtattgc ggaatagcat tagtaaattc ttgcaaggtg
     4321 ttattaataa tgcctgctaa gaaactgaat cctaagataa aaacaataag taaaatggta
     4381 atggctaacc ataatggcac tttacgctta gtcataaatt tgatcattgg tgaacaaata
     4441 atggcaataa atagcgaaag taaaaatggc acaacaattt cattggataa tttaatccct
     4501 gccagaataa tgataatgga ggctatcgcc acgagagttt tggtggaatg tgtcataatt
     4561 tttcctttta caagcggtta aaaatcagca attttttaca aattagtgat tctcaaacca
     4621 actttctaaa attaaacaag cggaaatgga atccacttta tcctttttta atgctttgta
     4681 accaccacga gaaaaaattt ccgcttttgc ttcaacagta gtgaggcgtt catcttgtag
     4741 ctcaaccggc agattaaagc gtccgtttaa gcgattggca aattttctgg cacgctgagt
     4801 taaaggctgc tctgtgccgt ccatattcaa gggtagccca acaaccagca tatctggttt
     4861 ccaatcttgc aagagttttc cgatctcttc ccagtttgga ataccgtctt gggctttaaa
     4921 tgtagttaaa ccttgagctg tgccggtaat actttgtcca actgcacagc cgatgctatt
     4981 tgtgccaaaa tcaaaggcaa tcacggtttt tgttgtggtt tctgtcatta agctctccca
     5041 gctcttgacg gtatgcttga aagccccatc atttcatagg cttctgccca acgctctaaa
     5101 taaccggttt caaacagcgt tttttcattc gcatcagaca caatccaata attttgagca
     5161 atttcttgct caagctggct gccacgccaa gtggaacaac ctaaacacac aataaatttt
     5221 tcaggtgaag ccagtgtgcc aaaggagtct aacacatcac cggaggtagt gagcgtaatt
     5281 ttatcggtga tcggataagt gtgaataaaa tcattggcgg ttttggtgtg aataataaag
     5341 ccacgatctt gatttaccgg cccaccgctt aacaccattt ggtctttttt gtaatcacgc
     5401 tggttcgcca tttggaaatc cattcgggta atcagctcta gaaccgacag atcggtagga
     5461 gtgttaatta ctactcccat tgcaccttgc ggattatgtt cgcaaatata gaccaccgta
     5521 cgctcgaaat agtcatcgtc catttccggc gtggcaatta aaaatttacc ttgtaaattc
     5581 tctaacatta aatatctcca aaacaaactt gtaacgcagt aattgccgca agtgaggcgg
     5641 tttctgtgcg taacactcgt ttacccagta atacttcagt aaaaccttcg ctctcggtca
     5701 tggcgatttc ttcggcagat aatccgccct cagagccaat cagtaaacga acaccagctt
     5761 tcggcacatt cggcagttca cgaatggtat atttggctct tgggtgcaaa ttcagtttca
     5821 acatatcgtc ttgttctttg caccagtcgg tgagtttcat aatcgggcga atttcaggga
     5881 tttcatttcg tccgcattgt tcacaggcgg caatcgcaat tttctgccat tgctgtaatt
     5941 ttttatcttg ccgttcatca ttgagtttca cgccacaacg ttccgaccaa agtggggtaa
     6001 tagtttttac ccccagctcc accgattttt gaatggtaaa ttccattcta tcgcctcggg
     6061 aaatcacttg ccctaaatgg atcggcaggt tggattctcg gtcatctaat tcacttgaat
     6121 caatttttgc aataatttgc tttttttcga ccgcttgtaa cgtagcgtgg aaaacgtggt
     6181 ttgaaccatc aaataaaatg atttgctctc cttgtttcat tcgtagcact cgcccaacgt
     6241 ggttagcggc atcttcactt aacacgcaac tttgaagacc agctaacggc tgtggttggt
     6301 aaattcttgg gattctcata attatctagt tataaattaa aggcagacga ttgtctgcca
     6361 taggtaagtt aaaacgggtt aaggataaag tcttgaacaa tgcacaatag taataatcga
     6421 aatttcattc tctttaactt
//
LOCUS       DANLXF010000135         6342 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.135, whole genome shotgun sequence.
ACCESSION   DANLXF010000135 DANLXF010000000
VERSION     DANLXF010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6342
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.135"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            11..1828
                     /locus_tag="PXD66_001722"
     CDS             11..1828
                     /locus_tag="PXD66_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HDL6320003.1"
                     /translation="MIIKDTHWTSQQASPNAFPERQALMPIWGGKNIERNQWQSLWLE
                     QAPNALLQDGLAYFHIPFCASHCIFCGFYRNAWKENYSQIYTDRVISELRYESSLWRG
                     QGKIHAVYFGGGTPTALHTEDLVRLIRACYRYLPLAEDCEFTIEGRISHFDIDKAKAC
                     IEAGVNRISIGIQTFNSEIRKRLGRKHSGEEAQTYLKALSELNAVIVADLIFGLPNQT
                     DDIWQNDIEIATSLPISGLDIYAFNNYPFLPINRLIEHGKLPAPAGFDTQSLHYAYAV
                     EKLKAQGWQQVSNNHFAFPNRGERNRYNTLVKSNMPCLAFGSGAGGNFGGLSYQVQSD
                     LKTYLDSPLDQKSLSFLSKHGEHKALLGVIQHSMELGYLDTTPFEQNAKAEKLLQQWE
                     ALDLLKREKNQVYLNTSGRYWSPTLIRKLMLTLPQSSKDNNMITPLTKELENKLRTNL
                     KENPGQILEMLAGMNQCSLESVINCLPKEMVMQTGGERFVEIMQAIAGWNEAVTFIIH
                     TPDAIAEVTAKLPQGKIGRGFYNFEHGVDGGIHGHLRYENCAAIYLLDRPFMGKRTVA
                     LTFINQSGEAMFKIFVGRDEQGQLRENQIQAMRKLIGGC"
     gene            1831..2448
                     /locus_tag="PXD66_001723"
     CDS             1831..2448
                     /locus_tag="PXD66_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL6320004.1"
                     /translation="MILVCGANGLLGRAICDKLPREKTIAVLRKDAMDTFFSERNIAV
                     EIADLLDLESCNALLQKTQPEILITVVGGKNEQGIRSDKTANINLIKAVEKYSPTTKV
                     VLVTSVGCAEQWDLISPLAQQALGEALKAKTEAEAYLQQTALNWLIIRPGGLNNDEVN
                     DEYQIVRDLPNERKMYVSRKSVATGIVRLLDSTENHQIFSIINRG"
     gene            complement(2532..3143)
                     /locus_tag="PXD66_001724"
     CDS             complement(2532..3143)
                     /locus_tag="PXD66_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentahemic C cytochrome"
                     /protein_id="HDL6320005.1"
                     /translation="MKSLSKIAFCFLAFIMPTFCFAGSNSYTTETIPLFADENLTQKI
                     GQLEAGAEIKLVQTTAKAAEIELNLWRKSKGFGRIWYHSFSKNITDAVFEKSFISNNP
                     LLEIVESKEDPNTGLVWQKVKMNVWVENAEFTQDLTNFWANTKNTYKTECSVCHKQRD
                     PKMHDANEWIAVFNGMVGFTDMDEATQKKVLRYLQMNAASSSQ"
     gene            3338..4246
                     /gene="glyQ"
                     /locus_tag="PXD66_001725"
     CDS             3338..4246
                     /gene="glyQ"
                     /locus_tag="PXD66_001725"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017806840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDL6320006.1"
                     /translation="MSTKFNVKTFQGMILALQDYWASKGCTIVQPFDLEVGAGTSHPM
                     TCLRALGPEPMAFAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLDS
                     LKMLGFDPTQNDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPV
                     TGEITYGLERLAMYIQGVDSVYDLVWSDGELGKTTYGDVFHQNEVEQSTYNFEYADVD
                     FLFQCFEQYEKEAQYLLGLEKPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILR
                     IRALTKGVAEAYYASREALGFPGCKK"
     gene            4326..4586
                     /locus_tag="PXD66_001726"
     CDS             4326..4586
                     /locus_tag="PXD66_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320007.1"
                     /translation="MKPILYFADWCPDTAPFVAELDRLGIEYEPCDITKGGSTLKPFL
                     KLRDTHSAFDHAKANGYIGIPALLIEGEKVVLDLAELEGIFG"
     gene            4629..5663
                     /locus_tag="PXD66_001727"
     CDS             4629..5663
                     /locus_tag="PXD66_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HDL6320008.1"
                     /translation="MKTVIADNSAEFVNEISQLIQSSKQRMTVAVNAELTLLYWHIGK
                     RINDYILQGERAEYGQEVVKNLAQSLTEQFGKGWSKRHLNYTMQFATTFPNLEIVHAL
                     RAQLSWTHFKQIIHIDDPTKRGFYATMAAQERWSTRTLEDRIGSLLFERTAISKKPDE
                     TIIAELSLLREKGEYNQSMVLKDPYILDFLDLNDRYMERDLEDAILREIEQFLLELGA
                     GFTFVARQKRIQIDNDDFYIDLLFYNRKLKRLIAIELKTESFKHSHKSQMELYLAWLA
                     KYEQEADENPPLGMILCTGKKQEQVELLDLKNSDIHIAEYMTVLPSKAVLEQRLHLAV
                     ERAKERFLLK"
     gene            6041..>6342
                     /locus_tag="PXD66_001728"
     CDS             6041..>6342
                     /locus_tag="PXD66_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320009.1"
                     /translation="MSIKKLLSTTIDQLENIIQVSGESFEKITSDLAEKAKELNSEYN
                     IKCEKLGYAITLFEKNSEIEKRYISLLSDFDMDTSIKGFEKLSLLMNGKNDNARKEA"
BASE COUNT         2008 a         1162 c         1386 g         1786 t
ORIGIN      
        1 tgtttcgata atgattataa aagatacaca ctggacatct caacaagcct ctccaaatgc
       61 tttcccagaa cgacaggcac ttatgcctat ttggggtggg aaaaatattg aacgcaatca
      121 atggcaatct ttatggctgg aacaagcacc aaatgcgtta ttacaggatg gtttagctta
      181 tttccatatt ccgttttgcg ctagtcattg tattttttgt ggtttttatc gcaatgcttg
      241 gaaggaaaat tatagccaga tttatactga tcgtgtgatt agtgaacttc gatatgaatc
      301 ttcactatgg cgaggacaag gtaagattca tgccgtttat tttggtggag gaacgccaac
      361 agcgttacat acagaagatt tagtacgatt gattcgagct tgttatcgtt atttgccact
      421 cgctgaagac tgtgaattta ctattgaggg tagaattagc cattttgata tagataaggc
      481 taaggcttgt attgaagcag gcgtgaaccg tatttctatt ggtattcaga cttttaactc
      541 cgaaattcgc aaacgtttag ggcgcaaaca tagtggcgaa gaggctcaaa cttatttaaa
      601 agccttatcg gaattaaatg ctgtgattgt tgctgattta atttttggct tgccaaacca
      661 aacagatgat atctggcaaa acgatattga aattgcgact agtttaccga tctcaggctt
      721 agatatttac gcctttaata attatccttt cctgcctatt aatcgcttaa ttgaacatgg
      781 aaaattacct gcgcccgcag ggtttgacac ccaaagctta cactatgctt atgcagtaga
      841 gaagttaaaa gcacaaggtt ggcagcaagt gagcaataac cactttgctt ttccaaatcg
      901 aggtgaacgc aatcgctata atactttggt taaatcgaat atgccttgtt tggcatttgg
      961 ctccggtgcg gggggaaatt ttggtgggct gagttaccaa gtacaatctg atttaaagac
     1021 ctacttagat tcgcctcttg atcagaaaag cctttctttt ttgagtaaac atggtgaaca
     1081 taaagcactg cttggtgtga tacaacatag catggaattg ggttacttag atacaacgcc
     1141 ttttgaacaa aatgcgaaag ctgaaaaatt attgcaacaa tgggaagcgc ttgatttact
     1201 gaagcgtgaa aaaaatcaag tatatctcaa taccagtggt cgttactggt caccaacatt
     1261 aattcgtaaa ctaatgttaa cacttcctca atcaagtaag gataacaaca tgataacgcc
     1321 attaactaaa gaattagaaa ataaattgcg gacaaattta aaagaaaacc cagggcaaat
     1381 tttagaaatg ctggcaggaa tgaatcaatg tagcttggaa tcggtaatta actgtctgcc
     1441 caaagaaatg gtaatgcaaa caggtggtga gcgttttgtg gaaattatgc aggcaattgc
     1501 tggttggaat gaagctgtga cttttattat tcatacgcct gatgcgattg cagaggtaac
     1561 tgctaaattg ccacaaggta aaattgggcg aggtttttat aattttgaac atggtgtaga
     1621 tggaggaatt cacggtcatt tacgctatga aaattgtgct gctatttact tacttgatcg
     1681 cccatttatg ggaaaaagaa cggtggcgtt aacttttatc aatcaaagcg gtgaggcgat
     1741 gtttaagatt tttgttgggc gtgatgaaca aggtcaatta cgagaaaatc agattcaagc
     1801 catgcggaaa ttaattggag gatgctagtc atgattttgg tttgtggggc aaatgggttg
     1861 ttaggacgag caatctgtga caaattacct agagagaaaa cgatagccgt cttacgaaaa
     1921 gatgctatgg atacgttttt cagcgaacga aatatagcag tagaaatagc agatttatta
     1981 gatttggaat cctgcaacgc cttgttacaa aaaacacaac cggaaatctt gattacggta
     2041 gtaggaggaa aaaatgaaca aggtattcgt agcgacaaaa cagcaaatat taatttgata
     2101 aaagcagttg aaaaatattc accaactacg aaagtagttt tagttactag tgttgggtgt
     2161 gcagaacaat gggatttaat tagcccatta gcacagcaag ctttaggtga agcactaaaa
     2221 gccaagacgg aagctgaagc ttatttacag caaacagcat taaattggct gattattcga
     2281 cccggtgggt taaataatga tgaagtaaat gatgagtatc agattgtgcg tgatttacct
     2341 aatgaacgga aaatgtatgt aagccgtaaa tctgtcgcaa caggcatagt gcgtttactg
     2401 gatagtacgg aaaatcatca aatcttctct attattaata ggggatagta tatttcaacg
     2461 aaggcaagcg ggcatatttt atggttattt tgcaactata aaacagaccg cttgtgagat
     2521 ctttttctat tttattgaga actggatgcc gcattcatct gcaagtagcg aagcaccttt
     2581 ttctgggtag cttcgtccat atcggtaaaa ccgaccattc cgttaaatac tgcaatccat
     2641 tcgtttgcat catgcatttt aggatcacgt tgtttatggc acacgctgca ttctgtttta
     2701 taggtatttt tggtgttcgc ccaaaagtta gtgagatctt gggtaaattc ggcattttcc
     2761 acccacacgt tcattttcac tttttgccat accaagccgg tgttcggatc ttccttgctc
     2821 tctacaatct ctaacagtgg gttgttgctg ataaaagatt tctcaaatac cgcatcggta
     2881 atatttttac tgaaagaatg ataccaaatc cgcccgaaac ctttgctttt tcgccataaa
     2941 ttcagctcaa tttcagcggc ttttgcggtt gtttgcacca atttaatttc tgctccggct
     3001 tctagctgcc cgattttctg tgtcaaattt tcatcggcaa ataaagggat tgtttccgtg
     3061 gtgtaactgt ttgaaccggc aaaacaaaat gttggcatga tgaatgccaa gaaacagaaa
     3121 gcgattttcg ataatgattt catcataaac cccacaaatt agatgagaaa ttaggttata
     3181 tgttacacaa attgagcaaa aaagcgttga tttacatcat aaaactgatt ttatgtcgat
     3241 ttcttaattt tttgcaaaaa aatcccaaaa aatgactgct tacaagctat aataaccagt
     3301 atttttcgac catttttcaa attcaagaga aaataaaatg agtacaaaat tcaacgtaaa
     3361 aacctttcaa ggaatgattt tagccctgca agactactgg gcaagcaaag gttgcaccat
     3421 tgtgcagccg ttcgatttag aagtgggggc aggcacctcg cacccaatga cctgcctgcg
     3481 tgcgcttggc cctgagccaa tggcgtttgc ctatgtgcaa ccatctcgcc gcccgacaga
     3541 cggtcgctac ggcgaaaatc cgaaccgttt acagcactac taccagttcc aagtggtgat
     3601 taaaccttcg ccggataaca ttcaagaact ttacctcgac agcctgaaaa tgctcggttt
     3661 cgacccaacc caaaacgaca ttcgttttgt ggaagacaac tgggaaaacc caacgcttgg
     3721 ggcgtggggc ttaggttggg aagtgtggct gaacggaatg gaagtgaccc aattcaccta
     3781 cttccagcaa gtcggcggct tggaatgtaa accggtgaca ggcgaaatca cctacggctt
     3841 agagcgtttg gcgatgtaca ttcaaggcgt ggacagcgtg tacgacctcg tgtggtctga
     3901 cggcgaactc ggtaaaacca cctacggcga cgtattccac caaaacgaag tggagcaatc
     3961 cacctacaac tttgaatacg ctgatgtgga cttcctgttc caatgcttcg agcagtacga
     4021 aaaagaagcc caatacctgt taggcctaga aaaaccattg ccattgccgg catacgaacg
     4081 cattttaaaa gccgctcaca gctttaactt attagatgct cgtaaagcga tctcggtaac
     4141 tgaacgccaa cgctacattc tacgcattcg tgccttaacc aaaggcgttg ctgaagcgta
     4201 ctatgccagc cgtgaagcgt tggggttccc ggggtgtaaa aaataatcgt agggacgcca
     4261 cgcttggcgt ccgtcaataa attacaaaac ggacacgaag catcgtgtcc ctacaaggat
     4321 ttacgatgaa accgatttta tacttcgccg attggtgtcc ggataccgcc ccatttgtgg
     4381 cagaactaga ccgcttgggc attgaatatg agccgtgcga catcaccaaa ggaggctcaa
     4441 cacttaaacc atttttgaaa ctgcgagaca cgcattctgc ctttgaccac gccaaagcca
     4501 acggctatat tggcattccg gcattgctga tcgaaggcga aaaagtcgtg ctggatttgg
     4561 ctgaattaga gggtattttc ggctaacaag cggttgaatt tggcggaaat gttgcaaagg
     4621 agccaataat gaagacagta atagcagata attcggctga atttgtgaat gaaatttcac
     4681 aacttattca atcgagtaag caacgaatga ccgttgcggt taatgcagaa ttaaccttgc
     4741 tttattggca tatcggaaaa cggatcaatg actatatttt gcagggtgaa cgtgcggaat
     4801 atgggcaaga agtggtgaaa aatcttgctc aaagtctgac ggagcaattt ggtaaaggtt
     4861 ggagtaaacg ccatttaaat tatacaatgc aatttgctac aactttccct aatttggaaa
     4921 ttgtgcacgc actgcgtgca caattaagct ggacacattt taagcagatt attcatattg
     4981 atgatccaac aaagcgaggg ttctatgcaa caatggctgc acaggagcgt tggagtacaa
     5041 ggacattaga agatcgcatt ggctcattat tgtttgaacg cacggcaatt tccaaaaagc
     5101 ccgatgaaac cattattgct gaattgagtt tgctacgtga aaaaggcgaa tataatcagt
     5161 caatggtgtt gaaagatccc tatattttgg attttctcga ccttaacgac cgttatatgg
     5221 aacgtgatct cgaagatgcg attctacgag agatcgaaca gtttttactg gaacttgggg
     5281 cgggttttac ctttgtcgcc cgccaaaaaa ggattcagat tgataatgat gatttctata
     5341 tcgatctgct gttttataac cgtaaactca aacgcctgat tgcgattgaa ctaaaaaccg
     5401 aaagcttcaa acattcccac aaaagtcaaa tggaactcta tttggcgtgg ctggcgaaat
     5461 acgagcaaga agcagacgaa aacccaccgc ttggaatgat tctctgcacc ggtaaaaagc
     5521 aggagcaagt ggaattgttg gatttgaaaa attccgatat tcatattgcg gaatatatga
     5581 ccgtgttgcc gagtaaagcg gtattggaac aacggttgca tttggcggtg gaaagggcga
     5641 aagagcggtt tttactaaaa tagcaagcgg ttaaaatcag cggaaatgtt gcaaaatgaa
     5701 agtatgatgg aaacgatttg gttgcatatt ccgttggtgg gtggttagag attttcaaat
     5761 ctcccccctc cccctctttg gctaaagagg gggcgatcat ttagtgagta attttaattc
     5821 atctgactat ttacaatctt aatgaagaaa gatgaattat aaatttacga agatcaaatt
     5881 ccccttttta gcaaagaggg gctaggggag atttggcaga agtgaaaacg tagtgagaga
     5941 ttgattagtc tagtactttt ttgcaagtta atcataaagt tgctctcatt tcttgataag
     6001 tacaataaaa gcaaagaaaa ggatataata aaggaaatat atgtcaatta aaaaattatt
     6061 atctacaact atagatcaac ttgagaatat aatacaagta tcgggcgaaa gtttcgaaaa
     6121 aattacaagt gacttagcag aaaaagcaaa agaattaaat tcagaatata atattaaatg
     6181 tgaaaaattg ggatacgcca taacattatt tgaaaaaaac tcggaaatag aaaaacgcta
     6241 catatcatta ttatctgact ttgatatgga tacaagtata aaaggatttg aaaaattgtc
     6301 tctactaatg aatggcaaga atgataatgc tagaaaagaa gc
//
LOCUS       DANLXF010000136         6260 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.136, whole genome shotgun sequence.
ACCESSION   DANLXF010000136 DANLXF010000000
VERSION     DANLXF010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6260
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.136"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            97..1212
                     /gene="asd"
                     /locus_tag="PXD66_001729"
     CDS             97..1212
                     /gene="asd"
                     /locus_tag="PXD66_001729"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDL6320010.1"
                     /translation="MQNVGFIGWRGMVGSVLMDRMVEENNFANINPVFFTTSQAGQKA
                     PVFGGKDAGELKNAFDIEELKKLDIIVTCQGGDYTNEVYPKLKETGWNGYWIDAASAL
                     RMKDDAIIVLDPVNQHVISEGLKNGIKTFVGGNCTVSLMLMAIGGLFEKDLVEWVSVA
                     TYQAASGAGAKNMRELLSQMGELEDSVKAELANPASSILDIERKVTAKMRAEDFPTEN
                     FGAALGGSLIPWIDKLLPETGQTKEEWKGYAETNKILGLSDNPIPVDGLCVRIGALRC
                     HSQAFTIKLKKDIPLAEIEQIIAAHNEWVKVIPNDKETTLRELTPAKVTGTLSVPVGR
                     LRKLAMGGEYLAAFTVGDQLLWGAAEPVRRILVQLTQ"
     gene            1322..2248
                     /locus_tag="PXD66_001730"
     CDS             1322..2248
                     /locus_tag="PXD66_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL6320011.1"
                     /translation="MKPIFLEIRHLRTLIALKESGSVSLAAKRVHLTQSALSHQIKLL
                     EEQYDLTLFERKSSPIRFTPAGERLIKLAYDILPKVIEAELDLARVKQGEVGEVRVAV
                     ECHTCFDWLMPAMDTFRQHWPLVELDIVSGFHTDTVGLLLSHRADLAIVAEEEDSAGI
                     IYKPLFSYEMVAICSKDHPLATKDVWEAEDFKDETLITYPVPDDMLDLLRKILGPAGI
                     KVTRRNSELTIAIIQLVASRRGIAALPYWAVKPYLDRGYVVARKITQQGLYSTLYGAI
                     READATLAYLDDFHDTVKSQSFATLPGLMVLE"
     gene            complement(2315..3760)
                     /gene="pntB"
                     /locus_tag="PXD66_001731"
     CDS             complement(2315..3760)
                     /gene="pntB"
                     /locus_tag="PXD66_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HDL6320012.1"
                     /translation="MSFGFVTAAYIIAAILFIMSLAGLSKHETAKAGCWYGIVGMAIA
                     LVATIFGPQSHGTIWILIAMAIGGFIGVKKALKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYGLHTNALMPANLDETAQAAFLAEQATLANIHNVEVFLGIFIGAVTFSGSLVAFG
                     KLSGKLFGRKVSSAALNIPHKHKWNLAAIIVSVFLMIVFLNNPHNIFPVLIMTIIALV
                     FGWHLVSSIGGADMPVVVSMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYI
                     MCKAMNRSFISVIAGGFGNEVQSGKGDEEYGEHRETKAEEVAEMLKNASSVIITPGYG
                     MAVAQAQYPVAEITAKLREKGVNVRFGIHPVAGRLPGHMNVLLAEAKVPYDVVLEMDE
                     INDDFEETDVVLVIGANDTVNPAALDDPSSPIAGMPVLEVWKAQNVIVFKRSMAVGYA
                     GVQNPLFFKENTQMLFGDAKERVDDILRALN"
     gene            complement(3775..5313)
                     /gene="pntA"
                     /locus_tag="PXD66_001732"
     CDS             complement(3775..5313)
                     /gene="pntA"
                     /locus_tag="PXD66_001732"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007525314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HDL6320013.1"
                     /translation="MLIGVPRELLDGETRVAATPKTVEQIKKLGFDVLVEQDAGFKAS
                     FEDNTFLNAGANIGTQQEVWNADIIFKVNAPTEAEIELIKEGVTLVSFIWPAQNPQLM
                     EKLQSKKINVLAMDAVPRISRAQALDALSSMANISGYRAVIEAANAFGSFFTGQITAA
                     GKVPPAKVLVIGAGVAGLAAIGAANSLGAIVRAFDSRPEVKEQVKSMGADFLEIDFEE
                     EGGSGDGYAKVMSEEFNRRAMALYAEQAKEVDIIITTAAIPGKPAPRLITKEMVDSMK
                     PGSVIVDLAAATGGNCEYTKAGEIVVTDNQVKVIGYTDFPARLPTQSSQLYGTNLVNL
                     LKLLAPNKDGQIEINFDDVVLRGVTVVRDGELTWPAPPIQVSAQLQKQAAAPQAVKKE
                     EKPADPRVKYGVMAAAGVLFLWLASVAPAAFLSHFTVFVLACVVGYYVVWNVSHALHT
                     PLMAVTNAISGIIIVGAVLQIAQPTGNFFIDILAFIAILVASINIFGGFKVTQRMLAM
                     FRKG"
     gene            5493..6209
                     /gene="trmJ"
                     /locus_tag="PXD66_001733"
     CDS             5493..6209
                     /gene="trmJ"
                     /locus_tag="PXD66_001733"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HDL6320014.1"
                     /translation="MQILNQIQIILVETSLPANIGSAARAMKTMGLTHLRLVAPKQPL
                     DEQAEALAAGAKDVLDNAQCYATFDEAIADCQLVIGTSARLRHLQNTLIEPRDCGELV
                     VNRTNLGKVAIVFGRERVGLTNEELLKCQYHLNFPTNPDYGSLNLAMAVQLASYEIRM
                     AYLQNSGEKQPLAADKDYPTAQALEHFFAHTERLYTELGFIRNDSVMLKLRRLYQRAE
                     LETNELNLLRGMLTAVEKAN"
BASE COUNT         1868 a         1388 c         1260 g         1744 t
ORIGIN      
        1 tcaaaacttt gcaaatcaat aaaaaacatt gatttttgca ctaaaatcct gtatttattc
       61 acaatcttta taacatcatt tttaggagca aacacaatgc aaaatgtagg ttttatcggt
      121 tggcgtggta tggtcggttc ggtcttaatg gatcgtatgg ttgaagaaaa taacttcgcc
      181 aatatcaacc ctgttttttt cactacttca caagcgggtc aaaaagcccc tgtttttggc
      241 ggaaaagatg ccggcgaatt aaaaaacgcc tttgacattg aagaattaaa aaaattagac
      301 attatcgtaa cttgccaagg tggtgattat acaaatgaag tctatccaaa attaaaagaa
      361 accggttgga acggctattg gattgacgcg gcttctgcat tacgtatgaa agacgatgct
      421 attatcgtat tagacccggt gaaccaacat gtgatttcag aaggcttaaa aaacggcatt
      481 aaaacatttg taggcggaaa ctgtaccgta agcctaatgc taatggctat cggtggttta
      541 tttgagaaag atttagtcga gtgggtttct gtagcaactt accaagccgc ttccggtgcc
      601 ggtgcgaaaa atatgcgtga attgctttct caaatgggtg aattagaaga tagcgtaaaa
      661 gctgaattag caaatcctgc ctcttctatt ttagatattg aacgtaaagt tacggctaaa
      721 atgcgtgcgg aagacttccc aaccgagaac ttcggtgcgg cattgggcgg tagcttaatt
      781 ccttggattg ataaattatt accggaaacc gggcaaacta aagaagagtg gaaaggttat
      841 gcagaaacta ataaaatttt aggtttaagc gataacccaa ttccagttga tggtttatgc
      901 gtgcgtattg gagcattacg ctgccacagc caagcattca caattaagct gaaaaaagat
      961 attccgttag cggaaatcga gcaaattatt gctgcccaca atgaatgggt gaaagtaatt
     1021 ccaaacgaca aagaaaccac attgcgtgaa ttgaccccgg cgaaagtgac cggtacattg
     1081 agcgtgccgg ttggtcgctt gcgtaaatta gcgatgggtg gagaatactt agctgcattt
     1141 accgtaggtg accaattatt atggggtgca gcagagcctg ttcgccgcat tttagttcaa
     1201 ttaacccaat aattcgccac tgctctatac aagcggttgg attttgcaaa ttttttgcag
     1261 aatttgaccg cttgtttgtt ataattattt tcatcttttt tcacttttag atgaataaat
     1321 tatgaaaccg atttttcttg aaatccgcca tttgcggaca ttaatcgcat taaaagagag
     1381 cggtagcgta tctcttgcag caaagcgtgt ccatttaaca caatccgcac tttcacacca
     1441 aataaaatta ctggaagaac agtatgatct tacgctattt gagcgaaaaa gctcccctat
     1501 tcgctttaca ccagctggcg aacgtttaat taagttggcc tacgatattt tgcccaaagt
     1561 cattgaggct gaattagatt tagcccgagt gaaacaagga gaagtcggag aggtacgtgt
     1621 tgcagtagaa tgccatacct gttttgattg gctaatgccg gcaatggata ctttccgtca
     1681 acattggcct ctagttgaat tagatatcgt atcgggtttc catactgata cggttggttt
     1741 gctattaagc catcgtgcag atctggcaat tgtcgctgaa gaggaagata gtgctggtat
     1801 tatatacaaa cctctctttt catatgaaat ggtagctatt tgctctaaag atcaccctct
     1861 tgccacaaaa gatgtatggg aagctgaaga ttttaaagat gagacattaa ttacctatcc
     1921 agtacctgat gatatgctag atttactgcg aaaaatatta ggccctgctg gcatcaaagt
     1981 tactcggcgt aatagtgagt taactattgc tattattcag ctagtagcaa gtcggcgagg
     2041 cattgctgca ctaccttatt gggcggttaa accatattta gatagggggt atgtggtggc
     2101 aagaaaaata actcagcaag gactatatag caccctttat ggcgctattc gagaagccga
     2161 tgctacccta gcctacttag atgatttcca tgataccgta aaatcgcaga gttttgcgac
     2221 tcttcccgga ttgatggtat tagagtaaaa aattaacaat ttatgttagc ctaagcacaa
     2281 aaaagcgaac ttatgttcgc ttttttattt aaatttaatt taacgcccgt aaaatatcat
     2341 caacacgttc ttttgcatca ccaaatagca tttgggtatt ttctttaaag aacagtgggt
     2401 tttgcacacc ggcgtagcct actgccattg aacgtttgaa cacgataaca ttttgtgctt
     2461 tccatacttc aagcaccggc attccagcaa tagggcttga tgggtcgtct aatgcagcag
     2521 ggtttacggt atcgtttgca ccgattacca ataccacatc ggtttcttca aaatcgtcat
     2581 tgatttcgtc catctccagc actacatcat aaggtacttt cgcttccgcc aataatacgt
     2641 tcatatgccc cggtaaacgc cctgccacag ggtgaatacc aaaacgcaca ttcacgcctt
     2701 tttcacgcaa ttttgcggtg atttccgcta ccggatattg tgcttgagcc actgccatac
     2761 cataacccgg cgtgatgatc acagagcttg catttttcag catttctgcc acttcttcgg
     2821 ctttcgtttc acggtgttca ccgtattctt catcaccttt gccagactga acttcattgc
     2881 caaaaccacc ggcaatcacg ctaatgaatg agcggttcat tgctttacac ataatataag
     2941 aaaggatagc acctgatgat cccactaatg caccggttac gattaataaa tcgttgctta
     3001 gcataaagcc tgccgctgcg gctgcccaac ctgaataaga attcagcatt gaaactacca
     3061 ccggcatatc cgccccacca atagaagaaa ctaagtgcca gccgaagact aacgcaataa
     3121 ttgtcataat cagcacaggg aagatattgt gcggattatt taaaaacacg atcatcaaaa
     3181 aaacagaaac gataatggct gccaaattcc atttgtgttt atgtggaata ttcagtgctg
     3241 cagaagagac tttacgacca aataatttac cgcttagttt accaaaggca accaatgaac
     3301 cggagaaggt taccgcacca atgaaaatac ctaagaacac ttccacattg tggatattgg
     3361 caagagtggc ttgttccgct aaaaatgccg cttgtgcagt ttcatctaaa tttgcaggca
     3421 ttaatgcatt ggtatgaagt ccgtagctgt taaaaccaac caataccgct gctaaaccga
     3481 caaagctgtg tagaattgcc actaattcag gcatttcggt catttctact tttagggctt
     3541 ttttcacccc gatgaatcca ccgattgcca ttgcgattaa gatccaaatc gtgccatgtg
     3601 attgcgggcc gaaaatggtt gccactaaag caatcgccat acccacaatc ccataccaac
     3661 aacctgcttt ggcggtttcg tgtttagaaa gaccggctaa gctcataata aagagaatag
     3721 ctgcaataat gtacgcagca gtcacaaatc caaaagacat agtttcgttt ctccttaacc
     3781 ttttctaaac attgcaagca tacgttgcgt tactttaaag ccaccaaaaa tattgatgct
     3841 ggcaaccaaa atcgcaataa atgccaaaat atcaataaag aaattaccgg tcggttgggc
     3901 aatttgtaat accgcaccta cgatgataat ccctgaaatc gcatttgtta ccgccattaa
     3961 tggggtatgt agtgcgtggc tgacgttcca aaccacataa taccctacca cacaagcgag
     4021 aacgaatacg gtaaaatgcg ataaaaacgc tgccggagcc acagatgcaa gccataggaa
     4081 cagcacacct gctgctgcca tcacgccata tttcactcga gggtctgcag gtttttcctc
     4141 ttttttgacc gcttgtggtg cggctgcttg tttttgcagt tgggcagata cttgaatcgg
     4201 cggagccggc caagttaatt cgccgtcacg caccaccgtt acgccacgca acactacatc
     4261 atcaaaatta atctcaattt gaccatcttt atttggtgct aacagtttga gtaaattcac
     4321 caagttagta ccataaagtt gcgaagattg cgttggtaaa cgtgcaggaa aatcggtata
     4381 gccaatcact ttcacttgat tatcggtcac cacaatctcg ccggctttgg tgtattcaca
     4441 gttaccaccg gttgcagcgg ctaaatcaac aatcactgaa cccggtttca ttgaatcaac
     4501 catctcttta gtgattaaac gaggagcagg tttgcccgga atggcagcgg tggtaataat
     4561 aatatccacc tctttggctt gctcggcata taacgccatt gcacgacggt taaactcttc
     4621 cgacatcact ttcgcatagc cgtcacctga gccaccttct tcttcaaaat caatttcgag
     4681 gaaatcagcc cccattgatt taacttgctc ttttacttca ggacgagagt caaaggcacg
     4741 cacaatcgcc cccaggctat ttgccgcccc gattgcagcc aagccagcaa caccggctcc
     4801 aattaccaac acttttgccg gtggtacttt acctgctgcg gtaatttgcc cagtaaagaa
     4861 gctaccaaaa gcgtttgccg cttcgatcac cgcacggtaa cccgaaatat ttgccatcga
     4921 acttaaagca tcgagagctt gggcacggga aatacgaggc acagcgtcca ttgccaacac
     4981 attaattttc ttagactgca atttttccat caattgtgga ttttgtgccg gccaaataaa
     5041 gctgactaag gttacccctt ctttgattaa ctcaatttca gcttctgtcg gcgcattcac
     5101 tttaaaaata atgtctgcat tccaaacttc ttgttgtgtg ccgatatttg cacctgcatt
     5161 taaaaatgta ttatcttcaa aactggcttt aaaacctgcg tcttgttcca caagtacgtc
     5221 aaagccaagt tttttgatct gctcaacggt ttttggcgtt gccgccactc gagtttcacc
     5281 gtctaacagc tctcttggta caccaataag cataaagact cctgtatggt tgttgtgatt
     5341 aaaaacgctc taaatgtacc actaaataaa acaaataggt aaaaaattat gcccaattaa
     5401 accgattatt tcccattttt tcaaatttta agcaggctaa atttttgcta cactattaac
     5461 aggctaaatt taacaaaaaa gaaacaaaac caatgcagat tctcaaccaa attcagatca
     5521 ttcttgttga aacttcactc ccggcaaata tcggttccgc agcccgagca atgaaaacga
     5581 tgggcttaac ccatttgcga ttagtcgccc caaaacaacc gcttgatgaa caagccgaag
     5641 cccttgctgc tggggcaaaa gatgtgttgg ataacgctca atgctacgcc actttcgatg
     5701 aagccattgc cgattgccaa ctggtgatcg gcaccagtgc cagacttcgc catttgcaaa
     5761 ataccctgat tgaaccaaga gactgtggtg aattagtggt aaatcgaacc aatcttggca
     5821 aagtggcgat tgtatttgga cgtgagcgag tggggctaac caacgaagaa ttattgaaat
     5881 gccagtacca tttgaatttc ccaaccaatc ctgattatgg ttctctgaat ttggcaatgg
     5941 cagtacagct cgcaagttat gaaatccgaa tggcttattt gcaaaattct ggcgaaaaac
     6001 aaccgcttgc agcagataag gattacccaa ccgctcaagc cttagagcat ttctttgctc
     6061 acaccgaacg cttatatacc gaattaggct ttatccgcaa tgattcagtg atgctaaaac
     6121 tccgccgttt ataccaacgt gccgaactgg aaactaatga gctgaattta ctgaggggaa
     6181 tgctcacggc ggtggagaag gcaaattgac aagcggttaa tttttagtag aaatttgcaa
     6241 aatccctgac taaaacaaaa
//
LOCUS       DANLXF010000137         6178 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.137, whole genome shotgun sequence.
ACCESSION   DANLXF010000137 DANLXF010000000
VERSION     DANLXF010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6178
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.137"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(29..427)
                     /gene="mutT"
                     /locus_tag="PXD66_001734"
     CDS             complement(29..427)
                     /gene="mutT"
                     /locus_tag="PXD66_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HDL6320015.1"
                     /translation="MSKPIIQVSAGIIRNEFGQIYLTQRLEGQDFAQSLEFPGGKVDP
                     NETPEEALKRELEEEIGIQVLSAFPYENFCFEYPTKIIEFFFYLVEEWVNEPYGREGQ
                     EGFWIAQSDLDEGAFPPANAQLIKRLKEHA"
     gene            complement(514..3240)
                     /gene="secA"
                     /locus_tag="PXD66_001735"
     CDS             complement(514..3240)
                     /gene="secA"
                     /locus_tag="PXD66_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDL6320016.1"
                     /translation="MISKLLTSIFGSSNDRTLKRLRKRVAQINKLEPTFEQLSDEALK
                     DKTAEFKQRLADGVSLDSLLPEAFATVREASKRVLGMRPFDVQLIGGMVLTGRNIAEM
                     RTGEGKTLTATLSCYLNALTGKGVHVVTVNDYLARRDAETNRPLFEFLGLTVGVNIPG
                     LPSDAKRAAYQADITYSTNSELGFDYLRDNLAHDKNERFQRELHYALVDEVDSILIDE
                     ARTPLIISGPAEDATQIYQAVDQIIPHLIAQDKEDSEEYTGEGDFTLDLKNKQAHLTE
                     RGMVKVEDFLTQMGLMEEGESLYHPARISLLHHVSAALRAHKLFERNVDYIVKDGEIV
                     IIDEHTGRTMAGRRWSDGLHQAIEAKERVAIQGENQTVASITYQNYFRLYEKLAGMTG
                     TADTEAFEFQQIYGLNTVVIPTNRPVIRDDRTDLMFKSEAEKFAAIITEIRDCIERKQ
                     PVLVGTASVEKSELLSAELTKAGIAHNVLNAKFHAQEAEIVAEAGAPGAVTIATNMAG
                     RGTDIVLGGNWKMEVAKLENPTQEQIDAIKAAWQERYDIVMQAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDPGSSRFYLSLDDALMRIYLNEGKLNMMRKAFTEEGEAMESKLLT
                     KVIASAQAKVEAHNFDGRKHLLQYDDVANEQRKVIYEQRNHLLETDNIASMIESIRQD
                     VFESTINQYIPPQSIEEMWNVPGLEERLRREFGLDLPISHWLETEKDLHEETLRERIL
                     NIAIAEYNAKEEIVGAEVMRNFEKGVMLQNLDELWKEHLASMDYLRKGIHLRGYAQKD
                     PKQEYKKESFAMFTNMLDTLKLNVISILSRIQVRSKEEVEEAQRQQAEMDAASTPVTQ
                     ATDSAAGSREENSAESLSEEELANLNIGRNDPCPCGSGKKYKHCHGNKARYS"
     gene            complement(3299..3619)
                     /locus_tag="PXD66_001736"
     CDS             complement(3299..3619)
                     /locus_tag="PXD66_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2547 family protein"
                     /protein_id="HDL6320017.1"
                     /translation="MSLFLKHFHRTPFWSQLFLGMVAILALPEIQVAYANESEQETVL
                     NQSFLQYAQSANDIEQQSLFIAELIHKIVEIKPQAVHFCEFFANSYRLDRQNAYPIRA
                     GPQT"
     gene            3740..4042
                     /locus_tag="PXD66_001737"
     CDS             3740..4042
                     /locus_tag="PXD66_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HDL6320018.1"
                     /translation="MKNSAPKKINDILQNSSLSRIVEKANFINDLNQKIQKRWPESYR
                     GLYRVVNLVDNQLIVSVKNATVRQGLILQQNMLLKQIQTDLPEITQLEFRVNPSVT"
     gene            4067..4441
                     /locus_tag="PXD66_001738"
     CDS             4067..4441
                     /locus_tag="PXD66_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein NlpE N-terminal
                     domain-containing protein"
                     /protein_id="HDL6320019.1"
                     /translation="MKKIALIGFTTAMLGACSLLPQKSVSGTYAGELPCADCEKIQAQ
                     LTLNADKTYQYDTVYFKDKKEYTYRDKGTYSWEPNKPNVIRLEKASGNLAFQVSEQHV
                     ELCDPNGNTVKNSNNYKLGKVR"
     gene            complement(4617..4706)
                     /locus_tag="PXD66_001739"
     tRNA            complement(4617..4706)
                     /locus_tag="PXD66_001739"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4670..4672),aa:Ser,seq:gga)
     gene            complement(4798..5109)
                     /locus_tag="PXD66_001740"
     CDS             complement(4798..5109)
                     /locus_tag="PXD66_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HDL6320020.1"
                     /translation="MSVEQTIIQKIKTEFQPIFFNVENESYMHSSGRGAESHFKLTLV
                     SDKFEGQRTVARHRAVYACLAEELANGVHALALHLYTQGEWEATGGLVPKSLNCLGVG
                     Q"
     gene            5201..5788
                     /locus_tag="PXD66_001741"
     CDS             5201..5788
                     /locus_tag="PXD66_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320021.1"
                     /translation="MKRSKKTLVLATTFVAALLAGCQSQSNTLTFSTPTPTAVFNTNN
                     QTAAVNVMTQDLRTSREIASYTKNGEVIRLTAVPEVAAMFQQAVQQDLNAKGFQIVQG
                     AANANVVVNIRKFFATVEQGNLRYKADAQVSVKVAVQGSKGNFSKNFNATRGYEGAFG
                     ASNDEIKKVLDQAYSDVVKAIYNDNEIANAIHQFK"
     gene            5960..6133
                     /locus_tag="PXD66_001742"
     CDS             5960..6133
                     /locus_tag="PXD66_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320022.1"
                     /translation="MNNPYHNVSQEYLDYLAKKVKQGKEDIKNGRIYSPEEVRKRVIA
                     AAFRGAENAKKVA"
BASE COUNT         1904 a         1315 c         1128 g         1831 t
ORIGIN      
        1 ttactttaga tacagttctc aatacaaatt atgcgtgttc ttttaagcgt ttaattaatt
       61 gggcatttgc cggtgggaaa gctccctcat ctaaatccga ttgtgcgatc caaaaacctt
      121 cttgcccttc tcttccgtaa ggttcattta cccactcttc caccaaataa aagaaaaatt
      181 caatgatttt tgttggatat tcaaagcaaa aattttcata cggaaaagcg cttaacactt
      241 gaataccaat ttcttcttct aattctcgtt ttagtgcttc ttcaggggtt tcattgggat
      301 cgactttccc cccgggaaat tcaagagact gagcaaaatc ttgcccttct aaacgttggg
      361 ttaagtaaat ttgcccaaat tcgttacgaa taatccctgc tgagacttga ataatcggtt
      421 ttgacatagt ttccttcact ctttctataa attcaagcgg tcatattaag cagaaaattt
      481 gcaaattcct gactgaatat gaccgcttgt taattacgaa tatctcgctt tgttaccatg
      541 gcaatgctta tattttttgc ccgaaccaca agggcaaggg tcatttcgac caatatttaa
      601 gtttgccaac tcttcttcac ttaagctctc tgcgctattt tcttctctac taccagcagc
      661 agaatcggta gcttgtgtaa ctggcgtact tgcagcgtcc atttcagctt gctggcgttg
      721 cgcttcttcc acctcttctt tgctacgcac ttgaatacgg cttaaaatgc taattacatt
      781 caattttaag gtatcaagca tattggtaaa catcgcaaaa gattcctttt tatactcttg
      841 cttcggatct ttttgtgcat aaccacgcaa gtgaatacct ttgcgtaaat aatccattga
      901 ggctagatgc tctttccaaa gctcatctaa gttttgtagc attacacctt tttcaaaatt
      961 acgcattacc tctgcgccta ctatttcttc cttcgcattg tactctgcaa tcgcaatatt
     1021 taagatacgt tcacgcaaag tctcttcgtg caagtctttt tcagtttcaa gccagtgaga
     1081 aatcggtaaa tccaacccaa attcacggcg taaacgctct tctaagcccg gcacattcca
     1141 catttcctcg atagattgag gcggaatata ttggttaatc gtgctttcaa atacatcttg
     1201 gcgaatgctt tcgatcattg aggcaatatt atctgtttct aatagatgat tgcgttgttc
     1261 gtaaattact ttacgctgtt cattggcaac atcatcatat tgtaataaat gcttacgccc
     1321 atcaaagtta tgcgcttcta ctttcgcctg tgcagaggca atcactttgg ttaacagttt
     1381 agactccatc gcttcacctt cttcggtaaa ggctttacgc atcatattta atttaccttc
     1441 attgaggtaa attctcatta gggcatcatc taaagagaga tagaaacgtg atgaccccgg
     1501 gtcaccttga cgaccagaac gacctcgcaa ctggttgtcg atacggcggg attcgtggcg
     1561 ttctgtacca ataatatgta aaccaccggc ttgcattacg atgtcataac gctcttgcca
     1621 cgcagcttta atcgcatcaa tttgttcttg tgtcgggttc tcaagcttgg ctacttccat
     1681 tttccagtta ccgccaagca caatatccgt accacgacca gccatattgg ttgcgatagt
     1741 aaccgccccc ggagctcccg cttccgctac aatttcagcc tcttgagcgt ggaatttcgc
     1801 atttaatacg ttatgagcaa taccggcttt ggttaattct gccgataata actctgattt
     1861 ttccaccgat gctgtgccaa ccaaaacagg ctgtttacgc tcgatacagt ctctaatttc
     1921 agtaataatc gccgcaaatt tctccgcttc gcttttaaac attaagtctg tacggtcatc
     1981 acggatcacc ggtctgttgg ttggaatcac caccgtattt aagccataaa tttgctggaa
     2041 ttcaaaggct tcggtgtctg ccgtaccagt cataccggct aatttttcgt acaaacggaa
     2101 atagttttgg taagtaattg aggcaacagt ttggttttcg ccctgaatag ccacacgctc
     2161 tttagcttca atcgcttggt gtaagccgtc agaccaacga cgtccagcca ttgtacgacc
     2221 ggtgtgctca tcaataatca caatttcacc gtcttttaca atgtaatcta cgttgcgttc
     2281 aaataattta tgggcacgca atgctgcact cacatggtgt aacagcgaaa tacgagcagg
     2341 gtgatataaa ctttcgccct cttccattaa ccccatttga gttaagaaat cctctacttt
     2401 caccatacca cgctcggtta aatgagcctg tttgtttttc agatctaagg taaaatcgcc
     2461 ctcaccggtg tactcttcac tgtcttcttt atcttgagca atcaaatgcg gaatgatttg
     2521 atctaccgct tgataaattt gggtcgcatc ttccgcaggc cctgaaataa ttaacggcgt
     2581 acgagcttca tcaattaaaa tagagtctac ttcatcaact aatgcgtagt gtaactcacg
     2641 ttggaaacgt tcgtttttat cgtgagctaa gttatcacgc aagtaatcaa aacctaattc
     2701 gctgttagtc gaataggtaa tatctgcttg gtatgcagca cgttttgcat cggaaggtaa
     2761 ccccggaata ttcactccaa cggttaagcc taaaaactca aataacggac ggttggtttc
     2821 cgcatcacgg cgggcgaggt aatcattcac cgtaacaaca tgaacacctt taccggtcaa
     2881 agcattcaaa taacaagata aagttgcagt tagggtttta ccttccccag tacgcatttc
     2941 tgcaatatta cgccccgtta gcaccatacc gccgatcagc tgtacatcaa atggtcgcat
     3001 acctaatact cgtttgcttg cttctctcac tgtcgcaaaa gcttcaggta ataggctatc
     3061 tagcgataca ccatctgcaa ggcgttgctt aaactccgct gttttatcct ttaacgcctc
     3121 atcagataat tgctcgaagg ttggttcaag cttattaatt tgtgccactc gctttcttaa
     3181 acgttttaaa gtgcgatcgt tgcttgagcc aaaaatagaa gtcagtaatt ttgaaatcat
     3241 tgtgttattc ctaaatataa aaatgggtta atcaattaat ggtaaaatta aattatattt
     3301 aggtttgtgg cccggctcga atggggtaag cattttgtct atccaagcgg taggagtttg
     3361 caaaaaattc gcaaaaatgc accgcttgtg gtttaatttc cacaatttta tggattaact
     3421 ctgcaataaa aagcgattgt tgctcaatat cattagcgga ttgagcatat tgaagaaaag
     3481 attgattaag aacggtttcc tgttcacttt cattggcgta cgcaacctga atttcaggta
     3541 aagcaagtat cgcaaccatt cccaaaaaga gttgcgacca aaaaggcgtt cgatgaaagt
     3601 gttttaaaaa taagctcatt aaattaagat aaatgaaaaa attgggctaa ttatacgcta
     3661 aagtaaagcc gaatgagtaa attattgggt caagtatcgt aaaatcaagc ctaaaataag
     3721 acgaaatgta aagaaacaga tgaaaaattc agcacctaaa aagattaatg acattttaca
     3781 gaactcaagc ttaagccgta ttgtcgaaaa agcaaacttc attaacgatt taaaccaaaa
     3841 aattcagaaa agatggccag aatcttatcg tgggctttac cgagtagtga atttagttga
     3901 taatcagctt attgtgagcg ttaagaatgc aaccgtacgg caaggtttaa tcttgcaaca
     3961 aaatatgtta ctaaaacaga tacaaaccga tttacctgaa ataacccaac ttgaatttag
     4021 agtaaatccg agtgttacat aatttaattt tgaagaggaa aatagtatga aaaaaatcgc
     4081 actcattggt tttaccaccg caatgctagg ggcttgctca ttgctaccac aaaaaagcgt
     4141 tagcggtact tatgcaggtg aattaccgtg tgctgactgt gagaaaatcc aagcccagct
     4201 aaccttaaat gcagataaaa cctaccaata tgatacggta tattttaaag ataaaaaaga
     4261 atacacttac cgagacaaag gcacatacag ttgggaaccc aataagccaa atgtgattcg
     4321 tctggaaaaa gcatcaggca atcttgcctt ccaagtgtct gaacaacacg ttgaactttg
     4381 cgacccaaat ggcaacacgg taaaaaacag caataactat aaactaggta aagtacgtta
     4441 aaagcagata aaaaggattc ttaggaatcc tttttctttg taatctatcg ccgatcgcat
     4501 aaaaaaaccc ccaagcaatg tttgctcggg gataacctaa aaatgaaaaa gtattatcta
     4561 ctttaactta accaaggaaa tatctatgaa aacagtaagg aatctttcag tatgaatggc
     4621 ggtgagaggg ggattcgaac ccccgataca gtttcccata tacacgcttt ccaggcgtgc
     4681 tccttcaacc actcggacat ctcaccgaat caacaaaaag atggcgtaaa tatagcaatt
     4741 ttacacggct ctttcaagta ttttcttcac aaaataaaat aaatcatttt tatccactta
     4801 ttgcccaaca cctaagcaat ttagcgattt aggtacaagt ccgccagtcg cttcccattc
     4861 accttgggta tataggtgca aagcaagtgc gtgaacgcca ttagctaatt cctcagctaa
     4921 gcaagcatag acagcacgat gacgtgcaac tgttctctgc ccttcaaatt tatctgacac
     4981 caaagttaat ttaaaatgcg attctgctcc tctacctgag ctgtgcatat agctttcgtt
     5041 ttcgacatta aagaaaatcg gctgaaattc tgtcttaatt ttttgaataa ttgtctgttc
     5101 taccgacatt ttttgctctc gttcattgaa aatcaaaacg aaataatact atactaagca
     5161 gaaattttct cgaaatttat tcaatataga aggatttact atgaaacgct ctaaaaaaac
     5221 gttagtgtta gccactactt ttgttgccgc attactagcc ggctgccaaa gccaatccaa
     5281 cacattaaca ttctctaccc caacgccaac tgctgtattt aacacaaaca atcaaacggc
     5341 tgcggtgaat gtaatgacac aagatttacg cacctcgcgt gaaatcgcaa gctacactaa
     5401 aaacggagag gtcattcgtt taaccgcagt gcctgaagtt gctgcaatgt tccaacaagc
     5461 cgtccaacaa gacctgaatg caaaaggttt ccaaattgta caaggtgcag cgaatgccaa
     5521 tgtggtagtg aatattcgta aattctttgc cacagtagaa caaggaaact tacgctacaa
     5581 agccgatgca caagtgagtg taaaagtggc agtgcaaggt tctaaaggaa actttagcaa
     5641 aaacttcaac gctacccgtg gttacgaagg tgcatttggt gcaagcaatg atgaaatcaa
     5701 gaaagtgtta gaccaagcct attctgatgt ggtgaaagct atttacaacg ataatgaaat
     5761 cgccaatgca attcaccagt tcaaataatt aattttttct ggcaagcggt catctttctt
     5821 cgattttttg caaaaaacgt ggaaaaatga ccgcttgttg ttttctcgtt ttgtgatgtg
     5881 gctcgaaaaa taagataaat taacttgatt aaacgatcag tagcccccac aatagagact
     5941 catcgcaggg aggaaaaata tgaataatcc ttatcataat gttagccaag aatatcttga
     6001 ttatcttgct aaaaaagtaa agcaaggaaa ggaagatatt aaaaacggtc ggatttattc
     6061 acccgaagag gtcagaaaaa gagttattgc tgccgctttt agaggtgctg aaaatgccaa
     6121 aaaagttgca taatatttat tatactgaag aggccttaat agatttagag aatattgc
//
LOCUS       DANLXF010000138         6159 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.138, whole genome shotgun sequence.
ACCESSION   DANLXF010000138 DANLXF010000000
VERSION     DANLXF010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6159
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.138"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            24..1940
                     /gene="iolC"
                     /locus_tag="PXD66_001743"
     CDS             24..1940
                     /gene="iolC"
                     /locus_tag="PXD66_001743"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HDL6320023.1"
                     /translation="MATVNHQDRPLDLICLGRVAVDFYGQQIGSRLEEMTTFSKYLGG
                     SSGNVAYGTAVQGLRSSMLARVGDEHMGRFLREELQSVGVDTSHLITDKERLTALVIL
                     GIKDKETFPLIFYRDNCADMAITKDDFSEAYIASSRCLAITGTHLSNPKTREAVLTAL
                     EYAGRNEVKRALDIDYRPVLWGLTSLGDGETRYIDSAEVTKSLQEVLHHFDLIVGTEE
                     EFHIAGGSTDTLTALKNVRKVSNAVLVCKRGALGCSVFEGELPNDLDGGLNVYGVRVE
                     VLNVLGAGDAFMSGLLRGYLNNEGWEQACHYANACGALVVSRHGCSPAMPTKRELDDY
                     LTRAESVPRPDLDERLNHLHRVTTRKKQWENLCIFAFDHRKQLVDMANKVGADLARIP
                     KLKQLLLKAMEQTATQSGLPQNQAGILADTTFGQDVLNEITGKDYWIARPIEEPSSRP
                     LELEYGDLGSQLASFPRDHVIKCLAFYHPNDESGLKARQDRKLKEVYQACCRTGHELL
                     LEIILPADMPQSEAFYNQAIAHFYQLGIKPEWWKLPDVSRAQWQAISATIEANDPDCR
                     GILILGLDAPLEVFKDTFEQAKGCEKVKGFAVGRTIFGEPSEKWLAGELDDNTLISAV
                     SEKYRTLIDLWQQR"
     gene            2229..3569
                     /locus_tag="PXD66_001744"
     CDS             2229..3569
                     /locus_tag="PXD66_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HDL6320024.1"
                     /translation="MSCNTSPKRFHASWESIKQHQVPEWYEDAKFGIFIHWGIYSVPA
                     FAPPTCQLGEIDIDEQWFCNNPYAEWYFNSINVKKGPTYEHHIKTYGESFAYNDFLPL
                     WKAEKWQPAEWAKLFAKAGAKYVVLTTKHHDGFCLYPSKYTDFNCMQAGPKRDLMGEL
                     TENVREQGLKMGAYYSGIIDWTYSSAPIFTESQNFSNACPTYEYADYAYKQVVELIDR
                     YKPDVLWNDIGWPKVGEHMLPHLFAHYYNSVQEGVVDDRWNKLWCDFTSKEYKHGVAS
                     RDKKWEMCRGMGLSFGYNQVEDESHLISVKDLISLLVETVADNGNLLLNIGPKADGTI
                     PQEQVERLLALGSWLEINGEGIYKSRCSQHQTEQIGDVTYRYTKVGADLYLYIDGLTE
                     AQISLPLSVYGELQALNTELKFTIENTAQGRVLHIQNYQPDWYVLGFKIADGEK"
     gene            3572..4912
                     /locus_tag="PXD66_001745"
     CDS             3572..4912
                     /locus_tag="PXD66_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL6320025.1"
                     /translation="MSVSQRFIDFMNAHVAPVARRMENQPHISAIRDGFIVVLPFLIV
                     GSFIMILLIPPFDENTQNAFGQAWWRFANWASPYGWNFFQMSFNAISLFTSASIAYNL
                     AKAYKREPLPAAFLSVMAFLLVAAPVKDGMMDIKFFGGIGLFSAIFIAIYSVEMTRLL
                     EFLKIKIRLPKEVPHAVAESLNIVIPILAILLTLYPFAIWVESATGRNIPQLIMDFMA
                     PLIAVSDSLGAICLFVIATHLLWFLGINGSLVLMQLWTPFLLQNMAANLAAFQAGEPL
                     PFIITNSFWDFYIVHGASGGVIALAFLLVRSKSAHLRSIGKIGLVPSFFSIGEPIVYG
                     VPMVVNPLFFIPLIFAPLANAVIAYLILDFNLIHRVYLMAPWTTPAPIGAYLVSAGDI
                     WAPILSIALIILDIVIYYPFFKMYEKVCVEKEKAQNLDEKAQQELLEKARMAAQ"
     gene            5050..5874
                     /locus_tag="PXD66_001746"
     CDS             5050..5874
                     /locus_tag="PXD66_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL6320026.1"
                     /translation="MNGPTQLAHFQEQIRKEYDGLSKRLKQVAQYVLDNGNSVVFDTV
                     STIAERADVPPSTLIRFANAFGFSGFNEIKQLYRQDMMEGTSRYTERVQLFRQIEPLD
                     EQNSELNHILDVFVQGNTQALQQLSTQVSEEQLAQTVDILHNAKRIFIVGLKRSFSIA
                     NYLNYALHHLDYDVFMIDGSGGMFDEQLSRIREGDAVIAVSFSPYANETLNVVTATAR
                     PGVKHIAITDSQLSPLLSFSDVSFIIKEAQVSGFRSQCATMTLAQTIAVSLALKKA"
     gene            complement(5918..6145)
                     /locus_tag="PXD66_001747"
     CDS             complement(5918..6145)
                     /locus_tag="PXD66_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HDL6320027.1"
                     /translation="MILEIINALGNKENIKQVEACLTRLRVVLHNNGLLNKSALKKLG
                     AVDVIKVGDTQQIIFGAKAGQYRDEINALIG"
BASE COUNT         1741 a         1314 c         1385 g         1719 t
ORIGIN      
        1 tttacttgtt gtaaggagcg gttatggcaa cagttaatca tcaagatcgt ccattggatc
       61 ttatctgttt agggcgtgtg gcggtggact tttatgggca acaaatcggt tcccgtttgg
      121 aagagatgac cacattttca aaatacctcg gcggctcgtc aggcaatgtg gcttacggta
      181 cggcggtgca aggtttacgt tcttcaatgc tggcaagggt cggtgacgaa catatggggc
      241 gttttctacg tgaagagcta caaagcgtag gcgtggacac cagccattta attaccgata
      301 aagaacgctt aactgcactg gttattctcg gcattaaaga taaagagact tttccgctga
      361 ttttctatcg tgataactgt gcggatatgg caatcaccaa agacgatttt agcgaagcct
      421 atattgcttc cagccgttgc ttagcaatta ccggcacgca cctgtcaaac cctaaaaccc
      481 gtgaagctgt gctgaccgca ctcgaatatg cgggtagaaa tgaggtaaaa cgagcgttag
      541 atattgatta tcgccctgtg ttatggggat taacctcact cggtgatggc gaaacccgtt
      601 atatagacag tgccgaagtc accaaatcgc tacaagaagt attgcaccat ttcgatctga
      661 ttgtcggcac agaggaagaa tttcatatcg caggcggttc gaccgatact ctcactgcac
      721 tcaaaaatgt gcgtaaagtc tctaatgccg tattagtgtg caaacgtggg gcattagggt
      781 gttcagtatt tgaaggcgag ctgccgaatg atttagacgg tggcttgaat gtgtacggcg
      841 tgcgtgtaga agtgctgaat gtgctgggtg caggcgatgc ttttatgtcg ggcttactgc
      901 gtggctactt aaataacgaa ggttgggaac aagcgtgcca ctatgccaat gcgtgcggag
      961 cgttagtggt atctcgtcac ggctgttcac cggcaatgcc gaccaaacgt gaacttgacg
     1021 attatttaac ccgtgcagag agtgtaccac gtccggattt agatgagcgt ttaaaccact
     1081 tacaccgtgt caccacccgc aaaaaacagt gggaaaatct ctgtattttt gcttttgatc
     1141 accgcaaaca gttggtggat atggcaaata aagtcggtgc agatcttgct cgcataccga
     1201 aactgaaaca gctgctatta aaagcgatgg aacaaaccgc aactcaatcc ggcttaccac
     1261 aaaatcaagc agggatccta gccgatacca ctttcgggca agatgtactt aatgagatta
     1321 ccggcaaaga ttactggatt gcccgcccga ttgaagagcc gtcttcccgc ccgctggaat
     1381 tagagtatgg agatttaggc tctcagctag catcattccc acgcgatcac gtcatcaaat
     1441 gcttggcatt ttaccacccg aatgatgaaa gcggtttaaa agcccgccaa gatcgcaagt
     1501 taaaagaggt gtatcaagcc tgttgccgta ccggtcacga gctattgttg gaaattattc
     1561 tgcctgctga tatgccacag agtgaggctt tctataacca agcgatagca catttctacc
     1621 aacttggcat taagccggaa tggtggaaat tgccggatgt ttctcgcgca caatggcaag
     1681 cgatttccgc caccattgag gcaaacgatc cggactgtcg tggtatttta attttaggtt
     1741 tagatgcccc gttagaggtg tttaaagaca cctttgaaca ggcaaaaggc tgtgagaaag
     1801 tgaaaggctt tgcagtcggc cgcaccattt tcggtgaacc gtccgaaaaa tggcttgccg
     1861 gtgaattgga cgacaatacc ttgatttctg cggtatcgga gaaatatcgc actttaatcg
     1921 atctctggca acaacgttaa tcatattgct ttaagtgaaa accccttagc ttatgatatt
     1981 aagcaagggg ttttgttttg caaaaaatcc ggctaaacag accgcttgct agccgtaaaa
     2041 atgcccgaat tttcctaaaa aagcgaatat cgggcattcc tcaatctgct tatcacaaaa
     2101 taagtgtgat gaagttcaca tttcatacat aaaccccccc ctttttatcg aaacaaattg
     2161 ttttattttt atcaaaatgg aaaatgtatt tcagtttagt tctatttaga ttcaatagga
     2221 gtctgtgtat gagttgcaac acttcaccaa aacgtttcca cgccagttgg gagtcgatca
     2281 aacagcacca agtaccagag tggtatgaag atgctaagtt tgggattttt attcactggg
     2341 ggatttactc cgtacccgcc tttgccccac cgacctgtca gttaggtgaa attgatattg
     2401 atgaacagtg gttctgtaat aacccttatg cagaatggta ttttaattcc atcaatgtca
     2461 aaaaaggccc gacctacgaa catcatatca aaacttatgg cgaatccttt gcctataacg
     2521 acttcctgcc actatggaaa gcggaaaaat ggcaaccagc agaatgggcg aaactgtttg
     2581 ccaaagctgg cgccaaatat gtggtattaa ccaccaaaca tcacgatggt ttctgtctct
     2641 atccaagtaa atataccgat ttcaactgta tgcaagccgg accgaagcgg gatttaatgg
     2701 gcgagctgac tgaaaatgtg cgggagcaag gcttaaaaat gggcgcttac tactcgggca
     2761 ttatcgattg gacttattcc tctgccccga ttttcaccga aagtcaaaac ttctcaaacg
     2821 cttgcccgac ttacgaatac gctgactacg cctataaaca agtggtagag ctgatcgacc
     2881 gctataaacc agatgtattg tggaatgata ttggctggcc aaaagtgggc gagcatatgt
     2941 taccacacct atttgctcat tattacaaca gcgttcaaga gggcgtagtg gacgaccgct
     3001 ggaacaaact atggtgcgat ttcacctcca aagaatacaa acacggcgtg gcttcccgtg
     3061 ataagaaatg ggaaatgtgc cgtggtatgg gcttatcgtt cggctataac caggtagaag
     3121 acgaaagcca tttgatttcg gtgaaagact tgatttccct attggtagaa accgtagccg
     3181 acaacggcaa tttattgctg aatatcggcc cgaaagcaga tggcactatt ccgcaagagc
     3241 aagttgaacg cttattagca ctggggagct ggctggaaat aaatggagag ggaatctata
     3301 aatcccgttg tagccaacac caaaccgagc aaatcggtga tgtaacctat cgttatacca
     3361 aagttggggc ggatctctat ctttacattg atggcttaac cgaagcccaa atcagcttgc
     3421 cactgtcagt ttatggtgag ctacaagcac tcaataccga gctgaaattt accattgaaa
     3481 acaccgcaca aggcagagtg ttacatattc aaaactacca gccggattgg tatgtattag
     3541 gctttaaaat tgcagatggg gagaaatagg tatgtccgtt tcacaacgtt ttattgactt
     3601 tatgaatgcc cacgttgctc cagtagcacg tcgaatggaa aatcaaccgc atatttccgc
     3661 tattcgagac ggttttattg tagtgttacc attcttaatt gtcggcagct ttattatgat
     3721 tttgctaatt ccgccatttg atgaaaatac ccaaaatgct ttcgggcaag cttggtggcg
     3781 ttttgcgaat tgggcaagcc cttacggttg gaatttcttc caaatgtcgt ttaatgcgat
     3841 ttcattattt acctctgcca gtatcgccta caaccttgcc aaagcctata aaagagagcc
     3901 cctgccagcg gcatttctct cggtaatggc atttttgtta gtggctgcac cggtcaaaga
     3961 cggaatgatg gatattaaat tctttggcgg tattgggcta ttctctgcga tttttattgc
     4021 catttattcg gtagaaatga cccgcttgtt ggagttctta aaaattaaaa tccgcttacc
     4081 gaaagaagtg ccgcacgctg tagcagaatc tctgaatatc gtgattccaa ttttagcgat
     4141 tttactgact ctctatccgt ttgccatttg ggtggaaagt gccacaggtc gtaatattcc
     4201 acaactaatt atggacttta tggcgccatt gattgctgtg agtgactcgc taggggcaat
     4261 ttgcttgttc gttattgcta cccatttgct ctggttctta ggcattaacg gctcattggt
     4321 actaatgcaa ctttggacac cattcctact gcaaaatatg gcggcaaact tagcagcatt
     4381 ccaagctggt gaaccgctcc cctttatcat caccaattcc ttctgggatt tctatatcgt
     4441 acacggggca agtggtggtg taatagcctt ggcattcctg ctggtacgca gtaaatcggc
     4501 acacttacgc tctatcggta aaatcggctt ggttccgtcc ttcttctcta tcggtgaacc
     4561 aattgtgtat ggtgtgccaa tggtggttaa tccgctgttc tttatcccgc tgattttcgc
     4621 accactagcg aatgcggtta ttgcctacct gattttagat tttaacctta ttcaccgtgt
     4681 ttacttgatg gcaccgtgga caacaccggc accgattggg gcatatctgg tttctgccgg
     4741 tgatatttgg gcaccaattt taagtattgc actgattatt ttggatattg tgatctacta
     4801 tccattcttc aaaatgtatg aaaaagtctg cgtcgagaaa gaaaaagcac aaaatctaga
     4861 tgaaaaagca caacaggagt tgctagaaaa agcacgaatg gcagcacaat aacacttatt
     4921 tctagcgtaa ttgagagggg ctaatcgaaa gattagccct tatttttgca aaaaattgac
     4981 aaaatttatc cgcttgtaaa caagaaaagc gtataatgaa ataaaaaatt caagcagaga
     5041 taatttgaaa tgaatggacc tactcaatta gcacattttc aagaacaaat ccgaaaagag
     5101 tatgacggac taagtaaacg tttaaaacaa gtggcacagt atgtgctgga taatggcaac
     5161 agcgtggtgt ttgatacggt ttctaccatt gctgaacgag ctgatgttcc cccttcaacc
     5221 ttaatccgtt ttgcgaatgc gtttggattt agcggtttta atgaaattaa acagctttat
     5281 cgccaagata tgatggaagg cacatcacgt tacaccgaac gtgtgcaact ttttcgtcaa
     5341 attgaaccgc ttgatgagca aaacagtgag ctcaatcata ttttagatgt gttcgtacag
     5401 ggtaatacgc aggcactgca acagttatcg acacaagtca gcgaagagca gttagcacaa
     5461 acggtagata ttttacacaa tgccaaacgc atttttattg tgggtttaaa acgctcattc
     5521 agcattgcga actacctaaa ctatgcgttg caccatttgg actatgatgt ctttatgatc
     5581 gatggttcgg gcggtatgtt tgacgagcaa ttaagccgaa tccgtgaagg tgatgcggtg
     5641 attgccgtga gcttttcccc gtatgcgaat gaaacactca acgtggtgac agcaactgcc
     5701 agaccggggg taaaacatat tgcgatcact gatagccaac ttagcccact cctttcattt
     5761 agcgatgtgt cgtttattat taaagaagca caggtgagtg gttttcgatc ccagtgtgcc
     5821 acaatgacgc tcgcacaaac gattgccgtt tcactggcgt tgaaaaaagc ctagattaag
     5881 aaagtgaaag acctgcgtaa atacaggtct ttgtctatta accgattaaa gcgttaattt
     5941 catcacgata ctgcccggct tttgcaccaa aaataatttg ttgcgtatca ccgactttaa
     6001 tgacatcaac cgccccaagc tttttcagtg ccgacttatt taataaaccg ttgttatgta
     6061 acaccacacg taaacgggtt aaacaagctt caacttgctt gatattctct ttgtttccta
     6121 aagcattaat aatttctaga atgatagatt tatcagaca
//
LOCUS       DANLXF010000139         6141 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.139, whole genome shotgun sequence.
ACCESSION   DANLXF010000139 DANLXF010000000
VERSION     DANLXF010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.139"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            39..209
                     /locus_tag="PXD66_001748"
     CDS             39..209
                     /locus_tag="PXD66_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320028.1"
                     /translation="MKNQRNDTQKSSLWQEFEYRKRLKNLIESGLSVAEIETRSQQIY
                     EELKSECTTEAN"
     gene            184..1053
                     /locus_tag="PXD66_001749"
     CDS             184..1053
                     /locus_tag="PXD66_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDL6320029.1"
                     /translation="MNAQPKQINPILKLHPKQSEAKKVSVDDGYTQIPNELLKAILRS
                     GVLGWKGSYLLATVLKTLSWHKESDWFTHSQVCEMMNIEPTKYHINQLSAARKELIRE
                     NILFEDGKETGVNLSVFEWKMVYPEKVGSSRNNRELIPEKVGNGYPEKVGNTKETITK
                     EKINNEFTNVNSVQQPETSLPKKTKSEPVDYQGVMEEFNRAVEDTPIPQIRAMSDERK
                     RSVHALAKILRKEFGGYTRQHFADYFNDFVRQASSRKDRFYFGGLDGSAWVANFGYII
                     RSKTFYKTWEDSL"
     gene            1050..2411
                     /locus_tag="PXD66_001750"
     CDS             1050..2411
                     /locus_tag="PXD66_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HDL6320030.1"
                     /translation="MSQNLKNITYEVEYLLVGALLKSGLNSKARDVLSWLEPEMFATF
                     QLGVIYEAIRKQALKDNVIDMLLLNTDYGQDFATLAEIMKNTISGANLDGYAEKVRQY
                     HQRREAQRVFLDVAGELQNARDEQLDAITSTGLAHLSKLLQRGGKVKPIDMNDLLEGY
                     LELFQERAKPSFKERLLFTGIEALDGKLGGINDTDICIVAGRAGNGKTETAITFTKNI
                     IENKGSVLFFSLEMSKEQIMDRLIASASGVNSVKLRNPEMMNDEDFARMGTAIQPLQN
                     QQLYIVDKSGLTAEEIVAIAESHIQEYGKVSAVVIDYIGLVRHGKLDGKINRTYQIGE
                     SMERFKTFCKNNHTPMILLAQLNRNADGSRPTNADLRDSGSLEQDASQIIMVHNQRNK
                     DGEPAPYTEWIVTKNRFGANGTVYMQFQQGRFVECDQAEAREYFQPKETKTSSNKRYG
                     AMQ"
     gene            2408..2944
                     /locus_tag="PXD66_001751"
     CDS             2408..2944
                     /locus_tag="PXD66_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HDL6320031.1"
                     /translation="MTDFDKNIKYKIIYADPPWKYKDSGCNGSAENHYTTMNIKEICD
                     LPVQDISDKDSILFLWVTYPMLPEGLKLIEAWGFNYKTIGFQWVKTNKKNKNSFFFGL
                     GRWTRGNTECCLIATKGKPSRVSNKVSQLIIEPIQHHSKKPDIVREKIVELMGDLPRI
                     ELFARNKTQGWDVWGDEV"
     gene            3035..3250
                     /locus_tag="PXD66_001752"
     CDS             3035..3250
                     /locus_tag="PXD66_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320032.1"
                     /translation="MITYKCPKCNGELEDLSINDEWGWFLDEPYRCNGHYTGRFPNIS
                     RDSTLNRTKSCGYFSKEEVKKVNGTQR"
     gene            3234..3740
                     /locus_tag="PXD66_001753"
     CDS             3234..3740
                     /locus_tag="PXD66_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HDL6320033.1"
                     /translation="MAHSVNDIVKAHGRRYKARLKIQMIKMQGGVLVPLNEREAEALV
                     SLKNGEQYEIEVIRTRNPAFHRKVFAFLNFCFEYWSADKTEWEFFDERKQFDTFRKNL
                     TVLAGFKEITYTLDGRMRVEAQSLSYGNMEQDEFEQVYKALINAAIKHVFNNTKDENR
                     INQLYSFF"
     gene            3790..4002
                     /locus_tag="PXD66_001754"
     CDS             3790..4002
                     /locus_tag="PXD66_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320034.1"
                     /translation="MKKNVIRYWLSVPFRIIAVLFFLVILTVRYVGYLLIYPLAFFAA
                     VGSFIVFCQWTYQEWLYYFKRGFAPI"
     gene            4048..4242
                     /locus_tag="PXD66_001755"
     CDS             4048..4242
                     /locus_tag="PXD66_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320035.1"
                     /translation="MTKFFETIAFVMLFGIIPSCLLAINTTLMNMNIKNAPWFMVIIQ
                     LGFNITCYWLWFISVMRNVI"
     gene            4370..4939
                     /locus_tag="PXD66_001756"
     CDS             4370..4939
                     /locus_tag="PXD66_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HDL6320036.1"
                     /translation="MANKPPKQHKCKECGSYYIKFQSTQQVCSVKCAMAMGKRKTEAK
                     RKKSAKIDRLEQRKRLEKLKSRRDFEKDLQKIFNKFIRLRDKDLPCISCGRYHQGKYD
                     AGHYKTVGGNPELRFNEDNCHSQCVPCNRHLHGNIVNYRVNLIEKIGLERVEFLERKD
                     HPPLKLTIEQIKDLIKVYKAKCKELERVT"
     gene            4929..5402
                     /locus_tag="PXD66_001757"
     CDS             4929..5402
                     /locus_tag="PXD66_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320037.1"
                     /translation="MLPRKIKNKWLEPEKQKWIEELLRLWGAWEFGGLDLESRVNMIY
                     RLMKSAEGVKSLSSREVCNDSLGTLINEIFTVVTKRDVMLADILKQKYWFGRSERQIA
                     IYYQLRDSEGRKERRWQEIIQDKCKKAERIIADILESKISSSNSTDKLKKYQFSP"
     gene            5630..5705
                     /locus_tag="PXD66_001758"
     tRNA            5630..5705
                     /locus_tag="PXD66_001758"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5663..5665,aa:Trp,seq:cca)
     gene            5722..5907
                     /locus_tag="PXD66_001759"
     CDS             5722..5907
                     /locus_tag="PXD66_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320038.1"
                     /translation="MYNLDDAIQECREKFGEDKPYFSEAGTLTFAGCKALSRLRDEKL
                     GIKAPPNFPFPRVEDLS"
     gene            5933..6103
                     /locus_tag="PXD66_001760"
     CDS             5933..6103
                     /locus_tag="PXD66_001760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320039.1"
                     /translation="MTEQLNTQSKSGEEDQEKSQDRYAVGWPSRRISDCVQHFRSRKI
                     YPFASNNDVKQG"
BASE COUNT         2014 a         1006 c         1438 g         1683 t
ORIGIN      
        1 caccgctgga acggtggtaa tttacaagga gttacctttt gaaaaatcaa cgcaatgata
       61 cacaaaaaag ctccttatgg caagagtttg aatatcgaaa acggttaaaa aatctcattg
      121 aaagcggttt atccgtagcc gaaattgaaa cccgttcaca acaaatttat gaggagctga
      181 aaagtgaatg cacaaccgaa gcaaattaac ccaattttaa aattgcaccc taaacaatca
      241 gaggctaaga aagtgagtgt tgatgatggt tatacacaaa ttccgaatga gttattaaaa
      301 gcaattttgc gatcgggtgt tttaggttgg aaaggctctt acttactggc taccgtctta
      361 aaaacattgt cttggcataa agaaagtgat tggttcacgc attcccaagt gtgtgaaatg
      421 atgaatattg agccgacaaa ataccatatt aatcaacttt ccgctgctcg aaaagaactt
      481 atccgtgaaa atatcttgtt tgaagatggt aaggaaacag gggtaaatct cagtgttttt
      541 gaatggaaaa tggtatatcc cgaaaaagta gggagttccc gaaataatag ggaattaatt
      601 cccgaaaaag tagggaatgg gtatcccgaa aaagtaggga acacaaaaga aactattaca
      661 aaagaaaaaa taaataatga atttactaac gtaaattctg tgcaacagcc cgagacctct
      721 ttgccgaaaa aaacaaaatc tgagccggtc gattatcaag gagtgatgga agaatttaat
      781 cgtgctgtgg aagacacacc gattccgcaa attcgggcga tgagtgatga gcgaaagcga
      841 tcggttcacg cattagcaaa aatcttgcga aaagaatttg gtggctacac cagacaacac
      901 tttgccgatt acttcaatga ttttgtccga caggcaagta gccggaaaga cagattttat
      961 tttggcgggc ttgatggctc ggcttgggta gcaaatttcg gctacatcat tcgttcgaaa
     1021 acgttttata agacttggga agattcgcta tgagccaaaa tttgaaaaat atcacttacg
     1081 aggttgaata tttgctggtt ggagcattgc tgaaatccgg tcttaactcg aaagctaggg
     1141 acgtattgag ctggttagag cctgaaatgt tcgctacctt tcaacttggg gtgatttatg
     1201 aggctatccg caagcaggcg ttgaaagata acgtgatcga tatgctgtta ctcaataccg
     1261 actacggaca agattttgcc acgttggcag agataatgaa aaacacgatc agcggtgcaa
     1321 acttagacgg ctatgcagaa aaagtccggc aatatcatca acgccgagaa gcccagcgag
     1381 tgtttttaga cgttgcaggt gagttgcaaa atgcccgaga cgagcagtta gatgctatca
     1441 cttcaaccgg gttagctcac ctaagcaaat tattacaacg tggcggaaaa gtgaaaccga
     1501 ttgatatgaa tgatttgctc gaaggttatt tggaattgtt ccaagaacgt gcaaaaccaa
     1561 gttttaaaga gcgtttgttg tttaccggta tagaagcatt agatgggaaa ctaggcggta
     1621 ttaacgatac cgatatttgc attgtagcag gacgtgccgg taacggtaaa accgaaacag
     1681 ccatcacgtt tactaaaaac attatcgaga ataaaggttc ggtgctgttc ttctcgctgg
     1741 agatgagcaa agagcagatt atggataggc tgattgccag tgcaagcggt gtaaattcag
     1801 taaaacttcg caatccggaa atgatgaatg atgaagattt tgcccgaatg ggaacggcga
     1861 ttcagccttt acaaaatcag cagttgtaca ttgttgataa aagcggatta accgcagaag
     1921 agattgtggc gattgcagag agccatattc aagagtacgg caaagtgagt gcggtagtga
     1981 ttgattatat cggtttggtt cgacacggca aactagacgg caaaattaac cgaacctatc
     2041 aaatcggtga aagtatggag cggtttaaaa ctttctgcaa aaacaatcac acaccgatga
     2101 ttttattggc acagcttaac cgcaatgcag atggcagtcg cccaaccaac gcagatttgc
     2161 gagacagtgg cagtcttgaa caagatgcaa gccaaatcat tatggtgcat aaccaacgca
     2221 acaaagacgg cgagccagcc ccctatacag aatggattgt tactaaaaat cgctttggag
     2281 caaatggcac ggtttatatg caattccagc agggtaggtt cgtagaatgc gaccaggcag
     2341 aagcgcggga gtatttccag ccgaaagaaa ctaaaacatc atcaaacaaa cgctatggag
     2401 caatgcaatg acagattttg ataaaaatat aaaatacaaa attatttacg cagatccgcc
     2461 ttggaagtat aaggatagtg gctgtaatgg gagtgcagaa aaccattaca caacaatgaa
     2521 tatcaaagaa atatgtgatt tacccgtcca agatatttca gacaaagatt ctattctgtt
     2581 tttgtgggtt acttacccta tgttacctga gggcttaaag ttgattgagg cgtgggggtt
     2641 caactataaa acaatagggt ttcaatgggt gaagacaaat aagaagaata agaattcttt
     2701 tttctttggc ttgggacgat ggactagagg taataccgaa tgctgtttaa ttgcaacaaa
     2761 aggaaagcca tcgagggtaa gcaataaagt gagccaatta attattgagc caatacaaca
     2821 tcatagtaaa aagcctgaca ttgtcagaga aaagatagtt gagttgatgg gtgacttgcc
     2881 aagaatagag ttgttcgcca gaaataaaac ccaaggttgg gatgtttggg gggatgaggt
     2941 gtgattgttt caagctattg ttacccaaag tgcggtaggg ttttattaaa aaggttggcg
     3001 gttgtgaagg ataacgaagg ttgggacggt ttagatgatt acttataaat gccccaaatg
     3061 caacggagag cttgaagatt taagtattaa tgatgaatgg ggttggtttt tagatgagcc
     3121 gtatcgttgc aacggacact atacagggcg gtttccaaac attagccgag atagcacgtt
     3181 aaatcgcaca aaatcctgtg ggtactttag caaggaagag gttaagaaag taaatggcac
     3241 acagcgttaa tgacatcgta aaagcccacg gcaggcgtta taaagcaaga ttgaaaatac
     3301 agatgataaa aatgcaagga ggcgttttag tcccccttaa tgagcgtgaa gctgaagcat
     3361 tggtatcact taaaaatggc gagcaatatg aaattgaggt tattcgcact cgcaatccag
     3421 cctttcatcg gaaggtcttc gcctttttaa atttttgctt tgaatattgg tcggcagaca
     3481 aaaccgaatg ggagtttttt gatgagagga aacagtttga tactttccga aaaaatctga
     3541 ctgttctggc aggatttaaa gaaatcacat acaccctaga tgggcgtatg cgtgtagaag
     3601 ctcaaagtct tagttacgga aatatggaac aagacgagtt cgagcaggtt tacaaagcat
     3661 tgattaatgc agctatcaag cacgttttca acaataccaa agatgagaat aggataaatc
     3721 agttgtattc gtttttttaa ttaaggcgaa atcgccatat ttaattaggt ttaattgcag
     3781 gagattaaaa tgaaaaagaa tgttattaga tattggctta gcgtgccatt cagaattatt
     3841 gcagtgcttt ttttcttagt cattcttact gttagatatg taggttattt attgatttat
     3901 ccattagcat tctttgcagc agtcggtagt tttattgttt tctgtcaatg gacatatcaa
     3961 gaatggcttt attattttaa gcgcggtttt gctccgattt agtgtgtgtt tattaattcc
     4021 ctcgaattcg ggggaaggag aagtaagatg acaaaatttt ttgaaactat cgcattcgtg
     4081 atgttatttg gcattattcc aagctgtttg ttggcgatta atactacttt aatgaatatg
     4141 aatattaaaa acgcaccgtg gtttatggtg attattcagt taggttttaa tatcacttgt
     4201 tattggttgt ggtttatttc tgttatgcgg aatgttattt aaggagatta ataagtgagc
     4261 cagtgcaatg aactgactgt aattttaatc gccatcgcta ttcttagtgt atgtgccggt
     4321 tcactctttt ttattgagtg gtttgataag agagatggtg agggttggaa tggctaacaa
     4381 accacctaaa caacacaaat gcaaagagtg cggcagttat tacatcaaat ttcaaagcac
     4441 gcagcaggtc tgctctgtta agtgtgctat ggcaatgggt aagcgtaaga cagaggcgaa
     4501 acgcaaaaaa agtgcaaaaa tcgaccgctt ggagcaacgg aagcggctgg aaaaattgaa
     4561 aagccgtaga gattttgaga aagatttaca aaagatattt aataaattca ttcgcttgcg
     4621 tgataaagat ttaccctgta tttcctgtgg ccgctatcat caggggaaat atgatgcagg
     4681 gcattataaa accgttggtg gtaatcctga gctacgtttt aatgaagata attgccacag
     4741 tcaatgtgtg ccgtgtaacc gacatctgca cggcaatatt gtgaattatc gagtaaatct
     4801 gattgagaaa atcgggcttg agcgtgtgga atttttagag cgtaaagacc acccaccatt
     4861 aaaacttact atcgaacaaa tcaaagattt aatcaaagtc tataaagcga agtgcaagga
     4921 gcttgagcgt gttacctaga aaaattaaaa acaagtggtt agagcctgaa aagcagaaat
     4981 ggatcgaaga gttgctaagg ttatgggggg cgtgggaatt tggcggattg gatttagaaa
     5041 gtcgggtaaa tatgatttac cggctgatga aatctgcgga gggggttaaa agtctttcat
     5101 ctagagaggt ttgtaacgat agcctcggca cgctgatcaa tgagattttt acggtagtca
     5161 ccaaaagaga cgtaatgtta gccgatatac tcaaacaaaa atactggttc gggcgttcgg
     5221 agcggcaaat cgctatttat tatcaactaa gggacagcga gggtagaaaa gaaagacgat
     5281 ggcaagaaat tattcaagat aaatgcaaaa aagcggaaag aattattgca gatattcttg
     5341 aaagcaagat ttcatccagt aattctacag ataaactgaa aaaatatcaa ttttcccctt
     5401 gattttagtg cgtaacaagt gtatattttg tgctaatggt ggacgtagta taaatgatgt
     5461 ttaccaatgt tagttaaaga cgcagcctga agtaatattc agttcggagg agaataacta
     5521 gaaacgacgt cgagtatagt tatttcagcg tttgcgtggc agtatgccga ccgaattagt
     5581 ttaacagccc tgagcagaaa tgcttggggc ttttttgttt acggatttta agggtgtagc
     5641 tcagttggta gagcagcggt ctccaaaatc gttggttgtt ggtttgagtc caaccaccct
     5701 ttccaaatgc gagtaaatgt tatgtataac cttgatgatg caattcaaga atgcagagaa
     5761 aaatttggtg aagataagcc ttatttttct gaagctggaa cacttacttt tgctggttgc
     5821 aaagccctct ctcgattaag agatgaaaag ttaggaatta aggctccgcc aaatttccct
     5881 ttcccaaggg ttgaagatct atcttagcta caaaaatcaa tgagggattg ctatgacgga
     5941 acaactaaat actcagtcaa aaagcggtga agaagatcaa gaaaaatctc aagatcgcta
     6001 tgccgtgggc tggccatctc gacgtatatc agattgcgtt cagcacttcc gaagtaggaa
     6061 aatttatcca tttgccagta ataatgacgt gaagcaaggt taaatgcttc tggtggtaca
     6121 gagtaaattc aatcattttt a
//
LOCUS       DANLXF010000140         6124 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.140, whole genome shotgun sequence.
ACCESSION   DANLXF010000140 DANLXF010000000
VERSION     DANLXF010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.140"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            4..216
                     /locus_tag="PXD66_001761"
     CDS             4..216
                     /locus_tag="PXD66_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL6320040.1"
                     /translation="MTNKKIQKAIELVGTQTKLAEACGVSQPTVLKWLNGGGISAEYL
                     LKIEAATNGQVTIREICEELGNATAK"
     gene            complement(315..560)
                     /locus_tag="PXD66_001762"
     CDS             complement(315..560)
                     /locus_tag="PXD66_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320041.1"
                     /translation="MNTEDQIIELTVEVENLRHQLNAKNIILDDLKGRLTDYQRKRLD
                     RLFERSRRQYLNDLDLEEEQAEALEAICNELESFLAN"
     gene            693..965
                     /locus_tag="PXD66_001763"
     CDS             693..965
                     /locus_tag="PXD66_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320042.1"
                     /translation="MNLDHTCTNCKSKNLRVRTSEKIGLLTIQAVTYCNNCGTEQLVQ
                     SQIIRVRTPIYKERPEAMRINKCLMEADLNTPDLFDGVVADQPKQD"
     gene            1048..1380
                     /locus_tag="PXD66_001764"
     CDS             1048..1380
                     /locus_tag="PXD66_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320043.1"
                     /translation="MSKQLYYRHKSRWNRLNRLKKMNKRRLNLFLQEKRVANLESRLA
                     DEVELAKLNSEQIGDLLQALELKVNRLIRQNEKLKARVIKLEKVKQTQKQGLFAMFKK
                     VLGSKGNP"
     gene            1393..1686
                     /locus_tag="PXD66_001765"
     CDS             1393..1686
                     /locus_tag="PXD66_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320044.1"
                     /translation="MDFVATYQKIRINTNHKQFTCNHLGEIWGKENDDFKRFLLRVAF
                     LQDNPEPKPWGAYSPQEKRLLHSFSLWLEQIADFQRVMKRESKLREKHINAPD"
     gene            1838..2080
                     /locus_tag="PXD66_001766"
     CDS             1838..2080
                     /locus_tag="PXD66_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320045.1"
                     /translation="MSLITVNDYKIKKDILDADGKPCYQLYQGEELLRDTTNLSEIIA
                     YMFIRAEDKPFDELYNHIINQEAGAKTDIKFTVTVR"
     gene            2077..2409
                     /locus_tag="PXD66_001767"
     CDS             2077..2409
                     /locus_tag="PXD66_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320046.1"
                     /translation="MSQITKSAYTHIHPNWQAPRGNTYRIERYELKRRYALFCNGERV
                     VLYKDPTHPNKNVESHIESDVLLLWLLKNELGWSGNIKEPLSPQDVENIRLLEQAFCL
                     TYNGTFIK"
     gene            2406..4766
                     /locus_tag="PXD66_001768"
     CDS             2406..4766
                     /locus_tag="PXD66_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HDL6320047.1"
                     /translation="MNYQNEILSATEAFAIGVSLPPVAGASACALELHSDRLYQAFKQ
                     PYTSMVQQELFSKDPIHEPLRQSYFERLPRALAEHFGRQYKQKLHYQSQYDTADWFKA
                     EMARKMPRIEAVISQYCDVFDFLKQSHQDLDFLAELDSNVYFGAGVDTQQAVQKIKQD
                     AEVKGIQLPMHLCRLENSSRYLKAHGIRPLAYQTEEQIKQLALGIAYRVRQIQQDNIE
                     QNMHKATDGDTAYSVLLECYRAMMGEVNKLKIDSPYQKKARKGRLTEEEITTGFLKMT
                     CEKWWTRKLSKIAEQMKEHLAIACGMVNMLSPYCSNARLKAFEAQRKANIDYLKSMII
                     ANIAEPEEQLSLFETWLKSASNPKIKRLELLTRMNGFERYADKQGHEGWFITLTAPSK
                     YHAMLSRTSSVNPKWNGASPAETQVYLVNTWAKIRAKLNREGVMAYGFRVAEPHADAT
                     PHWHLILFTRPEDMEKLRRVFLSYALEVDGTEAGAKKYRCKFKRIEKEKGSATGYLVK
                     YVSKNIDGFGMDGELSDEANIQAKENAARVGAWSSVWCIRQFQQLGNIPISLWRELRR
                     LGSVEQEDETLEKLRVIADSGEWDVFTEELGGALVKRADLVARITYTERKSETGEALY
                     TMYQEPSLKVSGIINIKNGVQINTRPKEWSIQLKPKNWEEQQLQKKLENATETERAEV
                     KRKYYEKLGFNEEAIALLGELAPPWTCVSNCTGSKNNQISEEARNCLRNELITMRGRV
                     TEHQIDDLLNGKRLKIWGNSQKTMFVSYSRGRLIEHIVDNYESFLN"
     gene            4779..5111
                     /locus_tag="PXD66_001769"
     CDS             4779..5111
                     /locus_tag="PXD66_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320048.1"
                     /translation="MVKFIRLNLINYFIEKDEYGEPVRCRAFRYDEIIQTKFIEGFRE
                     GEIPADDYGFEDWNGSVLILNEEGRKYFENYLFDVKKNYCRARDNYVYVLETLDEIEM
                     KLGLLEAK"
     gene            5111..5563
                     /gene="ssb"
                     /locus_tag="PXD66_001770"
     CDS             5111..5563
                     /gene="ssb"
                     /locus_tag="PXD66_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL6320049.1"
                     /translation="MNLVTLVGRLGQDPDIRTMQNGEKAAALSVATSEKWTDKQTGVK
                     KESTEWHRVVLYRRLAEIAELYVKKGHLVSIIGKIKTRKWTDSNGVERSITEIIAEQM
                     QMLSSGEKNTPNTPNKAENKPQPKQKNQDVMTADEQKDIPQFDDDIPF"
     gene            5574..5906
                     /locus_tag="PXD66_001771"
     CDS             5574..5906
                     /locus_tag="PXD66_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320050.1"
                     /translation="MREWEKQMPFSANLHIRPTGKFEVKNVNIEQIIGERGKNYGNFD
                     DVATMSQQLKAVLLDTNTQLKPHQKEAGEMICLKLARIFAGCNPDYEDNWRDIAGYAV
                     LGGKLNEK"
     gene            5896..>6124
                     /locus_tag="PXD66_001772"
     CDS             5896..>6124
                     /locus_tag="PXD66_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320051.1"
                     /translation="MKNKMGSKFIRVRVEHPQRKKEDIELVQDVVAYHIFNLFKFEES
                     GLRGFSQYDLEIDVKVKPVLEQKEMPVDVWID"
BASE COUNT         2013 a         1027 c         1458 g         1626 t
ORIGIN      
        1 tttatgacta ataagaaaat tcagaaagct attgaacttg taggaacaca aacaaaatta
       61 gctgaagctt gtggagttag tcagcctacg gtcttgaaat ggctaaacgg aggcggaatt
      121 tccgctgaat atctactaaa aatcgaagct gccacaaacg ggcaggtaac aattcgtgaa
      181 atttgtgagg agttgggaaa tgcaacagca aaataaaagc cctgaacagg cagggctagc
      241 gagtgggaaa tttactgtaa gacgtaatca agacggtaaa tggcgtttag atttgacctt
      301 gttggatttt ggtattagtt cgccagaaac gactcaagct cgttgcaaat tgcttcaaga
      361 gcttcagctt gctcttcttc aaggtcaagg tcatttaaat actgtcggcg tgaacgttcg
      421 aaaagccggt ccagtcgttt gcgttggtag tctgttaagc gaccttttaa atcatcaaga
      481 ataatgtttt tggcgtttag ttgatggcgt aagttttcaa cttcaacagt cagttcaata
      541 atttgatctt cagtattcat aaaatttcct tttggagggt taaacaatat ctaaaacata
      601 accaaaattc gactgcttgt ctatggggat agataagccg aaatcaacgt aaagaatgtt
      661 aatagagcct tggggaaggc gtaggggaaa taatgaattt agaccacacc tgtacaaatt
      721 gcaaaagtaa aaatttacgg gttcgtacat ctgaaaaaat cggtttgctg actattcagg
      781 ctgttactta ttgtaataac tgcggcacag agcaacttgt gcaaagtcaa attatccgtg
      841 ttcgcacacc gatttataaa gagcgaccgg aagcaatgcg tattaataaa tgcttgatgg
      901 aagccgattt gaacacgccg gatttgttcg atggcgttgt ggcagaccaa cctaagcaag
      961 actaatccca atttttaaac aatcgtcaaa ataagcctta tttgcatagg gcgagatttt
     1021 tgcaccctaa attcaggaat ttgaacaatg agtaagcaat tatattaccg acacaaatca
     1081 cgttggaaca gacttaaccg ccttaagaag atgaataaac gtcgccttaa tctgtttttg
     1141 caagaaaagc gggtggcgaa tttagaaagc cggttagctg atgaagtaga gctggcaaag
     1201 ctgaatagtg agcagattgg cgatttactg caagcgttgg agttaaaagt gaatcggtta
     1261 attcgccaaa atgaaaagct gaaagcccgt gtgattaagc tggaaaaagt caaacagacc
     1321 caaaagcaag gtttgtttgc aatgtttaaa aaggtgttgg gtagcaaggg taatccttag
     1381 taagaggtag atatggattt cgtggcaacg tatcaaaaga tacgcattaa cactaatcac
     1441 aagcaattta cttgcaatca cttaggcgag atttggggga aggaaaacga cgattttaaa
     1501 cgtttcttac tacgagtcgc attcttgcag gataaccccg agccgaaacc ttggggagcg
     1561 tattccccac aagaaaaacg ccttttacat agttttagct tgtggttaga gcagatagca
     1621 gattttcagc gagtaatgaa aagagaatca aaactgcgtg aaaaacatat taacgcaccg
     1681 gattagaagg agtaaatatg agccgtaacc gtgaattgtg tgagtttttt cacaaactgt
     1741 ggtgcaaaac cggtaatcgc tatgcttatc gccggtatca agtgtttttt cagcgttatc
     1801 aaaacgaatg tttagtgaat gagtagaggg tagaaaaatg tcattaatta ctgtgaacga
     1861 ttacaaaatc aagaaagata ttttagatgc agacggcaag ccgtgttatc agttgtatca
     1921 aggcgaagag ctattgcgtg ataccaccaa tttaagcgaa atcatcgctt atatgtttat
     1981 tcgtgccgaa gataaaccgt ttgatgagtt gtataaccat atcatcaacc aagaagcagg
     2041 agctaaaaca gatattaaat ttacggtaac ggtgagatga gccagattac taaatccgct
     2101 tacacacata ttcacccaaa ctggcaagcc ccgagaggta acacttaccg gattgagcga
     2161 tatgagctaa aacggcggta tgccttattt tgtaatggtg agagggtagt tttatataaa
     2221 gacccaacac accccaataa gaacgtagag agccatatag aaagtgatgt tttgttgtta
     2281 tggctactga aaaatgaatt agggtggagc ggtaatatta aagagccgtt aagccctcaa
     2341 gatgttgaga atatccgctt gttagagcaa gcattttgtt taacttataa cggaactttc
     2401 attaaatgaa ttatcagaat gagattctat ctgctacaga ggctttcgca ataggtgttt
     2461 cactcccacc tgttgccggt gcttctgctt gtgcattaga gcttcattct gatcgtttat
     2521 atcaagcgtt caaacagcct tacacttcaa tggtccagca agaattattc tcgaaagacc
     2581 ctattcacga accgttacgc caatcttatt ttgagcgttt gcctcgtgcc ttagcagagc
     2641 atttcggcag acagtataag caaaagctac attaccaaag ccaatacgac acagcagact
     2701 ggttcaaagc ggaaatggca cgcaaaatgc cacgcattga ggcagttatt agccagtatt
     2761 gcgatgtatt cgattttctg aagcaatccc atcaagattt agatttttta gctgaattag
     2821 atagtaatgt ttatttcggt gccggtgttg atacgcaaca agcggtacaa aaaattaaac
     2881 aagatgccga agtgaaaggt attcaactgc ctatgcacct ttgccgttta gagaatagct
     2941 ctcgctattt aaaagctcac ggcattcgcc cgcttgctta ccaaaccgaa gagcaaatca
     3001 aacagcttgc cttaggtatt gcttatcgtg ttcgccaaat tcagcaagat aacatcgagc
     3061 agaatatgca caaagcgaca gatggcgaca cagcctatag tgtgttgctt gaatgctatc
     3121 gggcaatgat gggagaggta aataagctca agattgattc gccttatcag aaaaaagcga
     3181 gaaagggtag attaactgaa gaggaaatta ccacaggctt tttaaaaatg acctgtgaaa
     3241 aatggtggac tagaaagtta tctaaaattg ccgaacagat gaaagagcat ttagctattg
     3301 cgtgcggtat ggtgaatatg ctttcgcctt actgttcaaa tgctcgcttg aaagcgtttg
     3361 aggctcaacg caaggcgaat attgattatt tgaaatcaat gatcattgcc aatatcgccg
     3421 agccggaaga gcaactttca ttatttgaaa cgtggttgaa gtctgcctct aacccgaaaa
     3481 ttaaacgcct tgaattactc actcgtatga atggatttga gcgttatgca gataagcaag
     3541 ggcacgaggg gtggtttatt accttgactg ctccgtctaa gtatcacgca atgctttcaa
     3601 gaaccagtag cgtgaacccg aaatggaatg gagcaagccc tgcggaaaca caggtttacc
     3661 ttgtgaatac ttgggcgaaa atccgtgcca aactcaaccg tgagggcgta atggcttacg
     3721 gttttcgagt ggctgaacct cacgctgacg ctaccccaca ctggcattta attctattca
     3781 ctcgtccgga agatatggaa aagcttcgcc gcgtgttctt gagttatgcc ttagaggtcg
     3841 atggcacaga agccggtgcg aaaaaatacc gttgcaaatt caagcgtatt gagaaagaga
     3901 aaggctcggc aacgggctat ttagtgaaat atgtttctaa aaacattgac ggttttggaa
     3961 tggacggcga gctttcagac gaggcaaata ttcaggcgaa agagaatgcc gctcgtgtag
     4021 gagcgtggtc tagcgtttgg tgtattcgac agttccagca gttaggtaat attccgatca
     4081 gcttatggcg tgagttacgc cgtttgggga gtgtagagca agaagatgaa actttagaga
     4141 aactccgtgt gattgcagat agcggtgagt gggacgtttt caccgaagaa ttaggcggtg
     4201 cgttggttaa acgtgccgat ttagttgcac gtattactta taccgaacgc aaaagcgaaa
     4261 ccggtgaggc gttatatacg atgtatcaag agccatcatt aaaagtgagc ggcattatta
     4321 acattaaaaa cggtgtgcag attaatacac gcccgaaaga gtggagcatt caacttaaac
     4381 ctaaaaactg ggaggagcaa caattacaga aaaaattaga aaatgcaacc gaaacggaac
     4441 gagcggaggt taaacgtaaa tactacgaga agttagggtt taacgaagag gctatcgctt
     4501 tattagggga gcttgctccc ccttggactt gtgtcagtaa ctgtacgggg tcaaaaaata
     4561 atcagattag tgaagaggct cgaaattgcc taagaaatga actgattaca atgcgaggac
     4621 gggttactga acatcaaata gacgatttac taaacggcaa gcggttaaaa atttggggaa
     4681 attcgcaaaa aacaatgttt gtgagttata gccgtgggcg tttaattgag catattgttg
     4741 ataattatga gagttttttg aattaggata ttaagaaaat ggttaaattt attcgtttaa
     4801 atttgattaa ttatttcatt gagaaagatg aatatggaga accggtgcgt tgcagagcgt
     4861 ttcgttatga tgagattatt caaactaagt ttattgaagg atttcgagag ggagagatac
     4921 cagctgatga ttatggattt gaagattgga atggttcggt tctgatttta aatgaagaag
     4981 gacgtaaata ttttgagaac tatctttttg atgtaaagaa gaattattgt agagcaaggg
     5041 ataactatgt atatgtcttg gaaactttag acgaaattga aatgaagtta ggattattgg
     5101 aggcgaaata atgaacttag tcacattagt cggacgtttg gggcaagacc ctgatattag
     5161 aacaatgcaa aatggtgaga aagctgcggc tttatcggta gcgacctctg aaaagtggac
     5221 agataagcaa acaggtgtga agaaagaaag caccgaatgg catagggtgg tgctttatcg
     5281 ccgattagct gaaatcgccg aattgtatgt aaaaaaaggg catttggtat caattatcgg
     5341 gaaaattaaa acccgaaaat ggacggatag caacggtgtt gagcggagta ttacagagat
     5401 tattgccgag cagatgcaaa tgctcagtag tggtgagaaa aatacgccaa atacgccaaa
     5461 taaggcagaa aataaaccgc aaccaaagca gaaaaatcaa gacgtgatga ctgcggacga
     5521 gcagaaagat attccgcagt ttgatgatga tattccgttt tagggggtga ataatgagag
     5581 aatgggaaaa acaaatgcca tttagtgcaa atttacatat aagacctaca ggaaaatttg
     5641 aagtgaaaaa tgtaaacata gaacaaataa tcggagaacg tggcaaaaat tacggcaact
     5701 ttgatgatgt tgccacaatg agccagcagt taaaagcagt gttgctggat accaatactc
     5761 aacttaaacc acatcaaaaa gaggcaggcg agatgatctg cttgaaactg gctcgcattt
     5821 ttgcaggttg taatccggac tatgaagata actggcgaga tattgcgggt tatgcggtgc
     5881 tgggagggaa attaaatgaa aaataaaatg gggagtaaat ttatacgagt cagagttgag
     5941 catccacagc ggaaaaaaga agatattgag cttgtgcagg atgtggttgc ttatcatatt
     6001 tttaatcttt ttaaatttga ggaaagtggg ttacgggggt ttagtcagta tgatttggaa
     6061 attgatgtga aagttaagcc agtgttagaa caaaaagaaa tgcctgtaga tgtttggatt
     6121 gatg
//
LOCUS       DANLXF010000141         6046 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.141, whole genome shotgun sequence.
ACCESSION   DANLXF010000141 DANLXF010000000
VERSION     DANLXF010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6046
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.141"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..140
                     /gene="zapB"
                     /locus_tag="PXD66_001773"
     CDS             <1..140
                     /gene="zapB"
                     /locus_tag="PXD66_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011201404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HDL6320052.1"
                     /translation="EELKGKNDAAKQENDTLRSEYEQLKVEQQNFQDRLRSLLGQIDN
                     V"
     gene            196..777
                     /locus_tag="PXD66_001774"
     CDS             196..777
                     /locus_tag="PXD66_001774"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014064645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDL6320053.1"
                     /translation="MAKLYFYYSSMNAGKSTTLLQSSYNYQERGMNTLVYTAAIDDRF
                     GVGKVNSRIGISQEANLFTADTNLFAEIKALHSNKALHCILIDEAQFLTKTQVYQLSD
                     VVDKLKIPVLCYGLRTDFQAELFEGSKYLLAWADELEELKTICDCGKKAHFVVRLNEK
                     GEAIKDGEQIQIGGNDRYLSVCRYHYKQKLGKL"
     gene            complement(868..1986)
                     /locus_tag="PXD66_001775"
     CDS             complement(868..1986)
                     /locus_tag="PXD66_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HDL6320054.1"
                     /translation="MTTTYFHLMAKPSSFHCNIQCEYCFYLSKEQTIPPEKSQFMNDE
                     TLQNYIRHYIEANQSQRVDFVWQGGEPTLLGLDFYKQAVKYQQKFANGKQITNAIQTN
                     GIALNRQWASFFKENHFLVGLSIDGLSDIHDRYRISTNGKPTFERVKSAVDLLKEVGV
                     EFNTLTVVNDRNWQRGRETYQALKALGSRYMQFIPVVESHKGELQPFSVPPQGYGQFL
                     VDVFHEWLPDRGQIFVIEFDNLLGQWLGYPSNNCVFRPTCGSSLVVEACGDVYSCDHF
                     VYPDYHIGNLNQQQLAEIVSSAAQRQFGQNKQAKLSQACLNCKFRILCHGGCPKHRIV
                     PQANSENHNYLCPSYQQFFAETSSVMRQMSEQIRFAGK"
     gene            complement(2076..3524)
                     /locus_tag="PXD66_001776"
     CDS             complement(2076..3524)
                     /locus_tag="PXD66_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDL6320055.1"
                     /translation="MNNIIYILLDQVRKDMLGTYGHQVVKTPNLDRLAKDGIRFNNAF
                     TPASVCGPARTSLFTGLMPSSHGIIKNGEKGGTGEIPQQNPNIAGVEGYNSFVIGKWH
                     VGTKSVPEDYGIAGHNFDGYGYPGSQVYKNLVFNQPPTHSNRYREWLAEKGYDAPEVS
                     RAYFGDNPHLRVQELCGLLSGSREQTIEWFIIDEAKKYIQASLDEKKPFFAWINFWGP
                     HTPCVVPEPYYSMYDPSQVVLDESFFKPLEGKPNHFRTISQMWGMWEASEDRWKEVIS
                     KFWGYITLIDDAIGELFRFLEENNLYENTFLVATADHGDAMGAHKMIEKGEFMFETTY
                     NIPLIIKDPHSTRVNEVDDNLVYLHDLTSTVYDVANQPIPTAFQGESILPITRQSQNN
                     DRKGLLCQLAGHFVYFEQRMWRRKDYKLVFNATDRCELYDVRKDPEEMHNLFYDEAYQ
                     AVKKEMLEEMRQEMKRLNDPLENWVYRIIDEI"
     gene            complement(3671..5368)
                     /locus_tag="PXD66_001777"
     CDS             complement(3671..5368)
                     /locus_tag="PXD66_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HDL6320056.1"
                     /translation="MLLTIITFLVVTGLVGYISWLKTRNDDLSTSKGYFLAGRGLSAT
                     VIGCSMVLTSLSTEQLIGVNANSYAGNFSIIAWTVPAVIPLCFLALYLLPKYIRNGYT
                     TIPEFFENRFDRQTRLIMSGLFLVFYLFIVIPTALYTGAIAFNKIFNLETAFGLSYGA
                     AITYTVIAIGIVGAIYAIFGGLKAVAVSDTWNAVLLVIGALLVPVFALIYLGNGSMAE
                     GVKIITTTHVEKWNAVGSATDAVPWPTIFTGIMVVHFFYWTTNQAIVQRALGARDLKA
                     GQKGILIAALFLLLLPLILNLPGLLSFHILGEGLKPIDVSYPELVNKTFPTVLQGFFI
                     AALFGAILSTFNSFLNSAATIYCKDLLPSISKKQFTDAELISYAKKVSAVMAVITMIF
                     GPLLMFGTDGIFLLTKRFAGFVNIPIVALFVIGLFNKTVSGKAARIALLAHVILYFCI
                     VWVFDVKINFVYVQAGLFVFDVALMLILGIFLKRATPYEDNKINHANVDLTSWEYAPL
                     AVASIVLGLFTLYAFLSPLGLASPDGNPAMVLAVYAVLQAVVCVVFFAKCKGKKAVN"
     gene            complement(5512..6033)
                     /locus_tag="PXD66_001778"
     CDS             complement(5512..6033)
                     /locus_tag="PXD66_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL6320057.1"
                     /translation="MSCQLSVMSDLEKGVVIYSQGYHAREFYFLKSGLIGLYHMLENG
                     KESLVRVYKANDYFGFRTFFGDNRYHCSARVLQPAELIRITPLNFIEFWSNNSALIIS
                     LLKQLSTELQDAEHRLSMVSYQKTQERVMNSIHYLHQRYPNYHWTNREIAEFAGCETE
                     TAIRLRRELKKIS"
BASE COUNT         1670 a         1358 c         1355 g         1663 t
ORIGIN      
        1 ttgaagaatt aaaaggtaaa aacgatgcag cgaaacaaga aaacgacacc ttgcgcagcg
       61 aatatgaaca attaaaagtg gaacaacaaa acttccaaga tcgtttacgt tcacttttag
      121 gtcaaatcga caacgtttaa ttctattttc agcacaaacg gctaaatttt tagccgtttt
      181 ttatttgtta cagctatggc aaaactctat ttttactact cctcaatgaa tgcaggcaaa
      241 tctactactc tgcttcaatc ttcttataat taccaagaac gtggaatgaa cactttagtt
      301 tataccgccg ctattgatga ccgtttcggt gtggggaaag tcaactctcg tatcggtatt
      361 tcacaagaag ccaatttatt taccgctgat accaatctat ttgcggaaat taaggcatta
      421 cactcaaaca aagcgttaca ctgtatcttg attgatgagg ctcagttttt aactaaaaca
      481 caggtttatc aattaagtga tgtggtcgat aaactcaaaa ttccggtgct ttgctacggg
      541 ctacgcaccg atttccaagc tgaattattt gaaggtagca aatatttgtt ggcttgggca
      601 gatgagctgg aagaactcaa aaccatctgt gattgtggca aaaaagccca ttttgtcgtt
      661 cgattaaatg aaaaaggcga agccattaaa gacggtgaac aaatacaaat cggcggcaat
      721 gatagatatc tctctgtttg ccgttatcat tacaaacaaa agttaggtaa gctataagct
      781 acaagaagca gcaagcggtt aaattttcgg gaatttttgc aaattttcag cgaaatttaa
      841 ccgcttgttt gtgttattta cctctcttta ctttcctgca aaccgaattt gctcactcat
      901 ttgtctcatc acactcgaag tctcggcaaa aaactgttgg taagaggggc aaaggtagtt
      961 gtggttttcg ctgtttgctt gggggacgat gcggtgtttg gggcagccgc cgtggcaaag
     1021 tatgcggaat ttgcagttta ggcaggcttg cgagagtttt gcctgtttat tctgcccgaa
     1081 ttggcgttgg gcggctgatg agacgatctc tgccagctgc tgttggttga ggttgccgat
     1141 gtggtaatcg ggatagacga aatggtcgca actgtacaca tcgccacagg cttccaccac
     1201 cagcgagctg ccacaggtcg ggcggaatac acagttgttg gagggatagc caagccattg
     1261 cccgagcagg ttatcaaact cgatcacaaa aatctgccca cggtcaggca gccattcgtg
     1321 aaaaacatcc actaaaaatt gcccgtagcc ttggggcgga acggaaaagg gttgtaattc
     1381 ccccttgtgg ctttccacca ccggaatgaa ctgcatataa cggctaccga gggctttgag
     1441 ggcttgatag gtttctctgc cccgttgcca gttgcggtcg ttcaccaccg tgagggtgtt
     1501 aaattccacg ccaacctctt tcaataaatc gaccgcactt ttcacccgtt caaaggtcgg
     1561 tttaccgttg gtggaaatgc ggtagcggtc gtgaatgtcg ctcagtccgt caatggataa
     1621 gcccaccaga aaatgattct ctttgaaaaa gctcgcccac tggcggttta gggcaatgcc
     1681 gttggtttgg attgcgttgg tgatctgctt gccgtttgca aatttttgtt gatatttgac
     1741 cgcttgtttg tagaaatcta agcccagcag ggtcggctcg cccccctgcc acacaaaatc
     1801 cacccgttgg ctttggttgg cttcaatgta atgacgaatg tagttttgca gcgtttcatc
     1861 gttcataaac tgcgattttt ctggcggaat ggtctgttcc ttgctcagat aaaaacagta
     1921 ctcgcactga atattacagt gaaaactgct gggttttgcc attaggtgga agtaggtggt
     1981 ggtcattgta tctctaaatt gtaggggcgg actttatgtt cgcccattgt ttacattaat
     2041 tttcgggcgg gtataaaacc cgcctctaca aacaattata tctcatcaat aatccgatac
     2101 acccaatttt ccagtgggtc gttgaggcgt ttcatttcct gtcgcatttc ttccaacatc
     2161 tcttttttca cggcttggta ggcttcgtca taaaagaggt tgtgcatctc ttcggggtct
     2221 tttctgacat cgtagagttc gcagcggtcg gtggcgttga agaccaattt gtaatcctta
     2281 cgccgccaca ttcgctgttc aaaatagaca aaatgccctg ccagttggca caacaagcct
     2341 ttgcggtcgt tgttttggct ttgtctggtt atcggcagaa tactctcgcc ttggaaagcg
     2401 gtcgggatcg gttgatttgc cacatcatac acggtggaag tgagatcgtg caaataaacg
     2461 aggttgtcat cgacttcgtt cacccttgta gagtgcggat ctttaataat cagcggaatg
     2521 ttgtaggtgg tttcaaacat aaactcgcct ttttcgatca tcttatgcgc ccccatcgca
     2581 tcgccgtggt cggcggtagc aacgaggaag gtattttcat agagattgtt ctcttccaag
     2641 aaacggaaca gctcgccaat ggcatcgtca atcagcgtga tgtagcccca gaatttagaa
     2701 atcacctctt tccaacgatc ttcgctcgcc tcccacattc cccacatttg tgaaatggtg
     2761 cggaagtggt tcggtttgcc ctcaagcggt ttgaagaaac tctcgtccaa taccacttgg
     2821 cttgggtcat acattgaata atacggctcc ggcactacac acggagtgtg cggcccccag
     2881 aagttgatcc acgcaaagaa cggttttttc tcatcaaggg aggcttgaat gtattttttc
     2941 gcctcgtcaa tgataaacca ctctattgtc tgctcgcgac tgccggaaag caaaccacag
     3001 agttcctgca cacgcaggtg tggattgtcg ccaaaatagg ctcgactgac ttcgggtgcg
     3061 tcatagccct tttccgccag ccattcacga taacggttgg agtgtgttgg cggctgatta
     3121 aacacgaggt ttttatacac ttggctgccc gggtagccat agccgtcaaa gttatgaccg
     3181 gcgatgccat aatcttcggg aacggatttt gtaccaacgt gccatttgcc gatcacaaag
     3241 gagttatagc cctccacgcc ggcaatatta gggttttgtt gtggaatttc gcctgtgccg
     3301 cctttttcgc cgtttttgat aatcccgtgg ctggacggca ttaagccggt aaacagcgag
     3361 gtgcgagccg gcccgcagac cgaagccggc gtaaaggcgt tgttaaaccg aatgccgtct
     3421 ttcgccaagc gatccaaatt cggggttttt accacctgat gcccgtaagt gccgagcata
     3481 tctttacgca cttgatctaa caaaatatag atgatgttat tcataatgac ctctttaagg
     3541 cttgttaata accgataaat tatcaagttt cggagatctg ttcccctctt tagcaaagag
     3601 gggatttttg atctcgagcc ttttccgttc ttggtatttt tatactaatg aggagaaggc
     3661 tcacattcat ttaattcacc gcctttttgc ctttacattt tgcaaaaaac acgacacaaa
     3721 ccaccgcttg tagcacagca tacaccgcca gcaccattgc cggattgccg tctggtgagg
     3781 ctaaaccgag cggagagagg aaagcgtata gcgtgaataa gcccaacacg atagaagcca
     3841 ctgccagtgg ggcatattcc cagctggtta agtccacatt ggcgtggttg attttgttgt
     3901 cttcgtatgg agtcgcacgt tttaggaaaa ttcctaaaat cagcattaac gccacatcga
     3961 acacgaacaa gccagcttgc acatacacga agttgatttt tacatcaaac acccaaacga
     4021 tgcagaagta gagaataacg tgtgcgagta aggcaatgcg tgcggctttg cctgagacgg
     4081 ttttgttgaa taagccgatg acaaacagtg ccacaatcgg aatgttcaca aaaccggcga
     4141 aacgtttggt cagcaagaaa ataccgtctg tgccgaacat cagtaacggc ccaaagatca
     4201 tcgtaatcac cgccattacc gcagagactt ttttcgcata acttatcagt tccgcatctg
     4261 tgaactgttt tttgctgata gacggcagta agtctttaca gtaaatggtt gccgctgagt
     4321 ttaagaacga gttgaaggta ctcaaaatcg ccccgaacag tgcggcaata aagaagcctt
     4381 gtagcacggt tgggaaagtt ttattcacta attcagggta agacacatca atcggtttca
     4441 agccctcacc taaaatatgg aaactgagta agcccggtaa gtttaagata agcggtagca
     4501 ataataagaa cagtgccgca attaagatcc ctttttgacc ggcttttaag tctctcgccc
     4561 ccaaggcacg ttgcacaatg gcttggttgg tcgtccagta gaagaaatgc accaccataa
     4621 taccggtgaa aatggtcggc caaggcactg catcggtggc actacccacc gcattccatt
     4681 tttcaacgtg agtagtggtg atgattttca ccccttctgc catactgcca ttacctaaat
     4741 agataagtgc aaacaccggc actaataatg ccccaatgac taacaagacc gcattccaag
     4801 tgtctgacac cgccaccgct tttaagccac cgaaaatggc ataaatcgcc ccaacaatac
     4861 caatcgcaat caccgtatag gtaatcgctg ccccatagct taagccgaag gcagtttcaa
     4921 ggttgaagat tttattgaag gcaatcgcac cggtgtaaag tgcagtcgga atgacgatga
     4981 aaagatagaa caccaaaaac aagcccgaca taatcaaacg ggtttggcgg tcgaaacggt
     5041 tttcaaagaa ctccggaatg gtggtataac cgttgcggat atatttcggc aacagataaa
     5101 gtgccaagaa gcaaagcgga attaccgccg gcacagtcca cgcaataatt gagaaattac
     5161 cggcataaga gttggcattc acgccgataa gttgctcggt agaaagggag gttaagacca
     5221 tcgaacagcc gataaccgta gcactcaagc cacgccctgc caagaaataa cctttagatg
     5281 tcgaaagatc gtcgtttctg gtttttagcc aagagatata cccgactaat ccagttacaa
     5341 ccagaaaggt aattattgtt aataacatat tcttcacctc aatatgacta tataaatgat
     5401 atatcccatt agagacggca atagcataaa tcttattttt aacgatagaa tatgatgttt
     5461 gtcataatag atatgtgatg gtgatcacaa ctatgaataa tttatgacaa tttaactgat
     5521 tttctttaat tctcgcctta atcgaatggc tgtttcggtt tcacaacctg caaattcggc
     5581 gatttcacgg ttagtccagt gataattggg ataacgttgg tgtaaataat gaatactgtt
     5641 cattacccgc tcttgcgttt tttgatagga aaccattgat aaacgatgct ctgcgtcttg
     5701 taattctgtc gagagctgtt ttaacagaga gataattaag gcggaattat tcgaccaaaa
     5761 ctcaatgaaa tttaaggggg taatacgtat caattctgcc ggctgtaaca cccgagccga
     5821 gcaatggtag cgattatcgc caaaaaaggt tctaaaccca aaataatcgt tggctttata
     5881 taccctcacc aagctctctt taccattttc aagcatatgg tacagcccta ttaatccgct
     5941 ttttaaaaaa taaaattctc tggcgtgata accctgagaa taaatgacga ctcctttttc
     6001 tagatcagac attacactta attggcaaga catcaattgt tcgggg
//
LOCUS       DANLXF010000142         6013 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.142, whole genome shotgun sequence.
ACCESSION   DANLXF010000142 DANLXF010000000
VERSION     DANLXF010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6013
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.142"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(52..1317)
                     /gene="wecC"
                     /locus_tag="PXD66_001779"
     CDS             complement(52..1317)
                     /gene="wecC"
                     /locus_tag="PXD66_001779"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HDL6320058.1"
                     /translation="MSNFNTISVVGLGYIGLPTATVFAQHGLNVIGVDVNQLAVDTIN
                     QGKIHIVEPDLDVAVHQCVSSGKLKATLTPEPAEAFLIAVPTPFKGEDYEPDLSYIQA
                     ACKAIAPVLEKDNLVILESTSPVGATEQMAEWLAEYRPELSFPQQAGEDSDIRIAHCP
                     ERVLPGQVIRELIENDRIVGGMTNKCSKQAVDLYKTFVKGDCIVTNARTAEMCKLTEN
                     SFRDVNIAFANELSIICDRLDINVWELIALANRHPRVNILQPGCGVGGHCIAVDPWFI
                     VNKTPDLAKIIRTAREVNDYKPEWVISKVNEAVIEALQKTGKSIDQIKIACLGLAFKP
                     DIDDLRESPALKITEKLAEKYVGQILAVEPNVEELPKKLAKKNISLVTVDEALKLADV
                     VVVLVDHREFRELAAIPSHITVIDTKGIY"
     gene            complement(1332..2444)
                     /gene="wecB"
                     /locus_tag="PXD66_001780"
     CDS             complement(1332..2444)
                     /gene="wecB"
                     /locus_tag="PXD66_001780"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDL6320059.1"
                     /translation="MRYLIVFGTRPEAIKMAPLVEGFKKNGLNFKVCVTAQHRQMLDQ
                     VLELFEITPDYDLDIMSNKQTLSTVTSAILEKIQPVIAEYKPTTIFVHGDTATTLATS
                     LAAYYNQIDIAHIEAGLRTHNIYSPWPEEGNRKLTGALAKYHFAPTQSTKKNLLNENI
                     TEDTIYVTGNTVIDALFFACKKLDENVDLLSSLQEKFSYIKSKRTILITGHRRENFGN
                     GFENICKAVSTLASDFPDVQFIYPVHLNPNVREPVNRLLADKSNVHLIEPCDYLSFVY
                     LMRESYLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVEAGTVKLVGTAQSEIIKQV
                     TELLNDQAAYTKMSEAHNPYGDGTAVKQILSVFQSK"
     gene            2677..4767
                     /locus_tag="PXD66_001781"
     CDS             2677..4767
                     /locus_tag="PXD66_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HDL6320060.1"
                     /translation="MLSYLVFFSKIIKIPNLKGFLPHCEIRRRPLVGKTFIAQKRPNQ
                     IIGWGLRPSAVGARAYAQKHQLPYIALEDGFLRSLGLGVKGYPPFSLVYDDIGIYYDT
                     TCPSRLEKLLLEKPLQDQEQIKLAERALSQILTYGLSKYNHALDFSEIPSAKEKIVLV
                     IDQTFGDMAVKYGQASEVDFNKMLETAISENPEAQIWVKTHPDVLSGKKKGYLTDLPQ
                     YTDRVRIITEDVNPASLIKLCEKVYCVTSQMGFEALLLGKKVVTFGVPWFAGWGVTDD
                     RNEAVQLLVQQNRRKDRSLLQLFYAAYFDYSRYINPNTGEKGSIFDVIEYLHKATILN
                     RRLSGNVYCIGMSLWKQAVIKPFFNLPQCRLHFVRSLNSLKKRPLADNAKILVWSNGK
                     ADVLQFAQQNNIPLLRMEDGFIRSVGLGANLVAPLSLVVDDLGIYFNAENTSRLEQIL
                     LSAEHFSETDLSYAETLRQKLVASHIGKYNVGGGDVKFNAQGKKIILVPGQVEDDASI
                     KSGSPQIKRNLDLLKKVRELNPSAYIIYKPHPDVVSGNRVGQIPTAETRRFADQMVDN
                     ANILDCILIADEVHTMTSLAGFEALLRGKKVHCYGLPFYAGWGLTEDRLKSARRGKSL
                     TLSQLIAATLIYYPLYVDPISRQFIDANQAIHILSQQKRNLTNQRIKRSWVSKQYGKL
                     QHLYRAFKYASHTK"
     gene            4790..>6013
                     /locus_tag="PXD66_001782"
     CDS             4790..>6013
                     /locus_tag="PXD66_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HDL6320061.1"
                     /translation="MEHYLDELIINSQRILLLQGPIGSFFTEFARWLESEQKEVFKLN
                     FNGGDEYFYPNNSPNTFPYYDSLDNFEAYLIQFCQTHQIDNILCFGDNRYYHKVAKNV
                     CLKLKLVFWAFEEGYFRPEYITLEKWGVNAFSPIPKNHTFFLPYAELPVVSEPEKVSK
                     GFRCMAGKAIPYYWNAYKKRKAYAKYQHHRFLNLKYYLNLWTRSGIKRICYWLHDKGF
                     AKRVEKGEFGEFFIVPLQVYDDTQVRIHSEQKSVERFLREVLNSFAKFAPAHLNLIIK
                     HHPMDRGFIDYKMVIYEFLEQYPQLNGRVFYIHDVPLPVFLRYGKGMVTLNSTSGLSA
                     LLHNMPVKTLGRANYDFEGLTDKQPLDTFWANPQPPNSEVFKGYRQFHLHKTHINGNF
                     YHKSILRYPYNWENEA"
BASE COUNT         1856 a         1040 c         1189 g         1928 t
ORIGIN      
        1 tctcgtgtta cacaacttcc aaaaatcaat attttcattt tatttcctta attaatagat
       61 acccttagta tcaataacag taatatgtga tggaatcgca gcaagttctc taaattctct
      121 atgatccacc aacacaacca ctacatcagc cagttttagt gcttcatcaa cagtaactaa
      181 agagatattt ttcttagcca attttttcgg tagctcttca acatttggct caactgctaa
      241 gatttgacct acatattttt ctgccaattt ttcggtaatt tttaaggccg ggctttcacg
      301 taaatcatca atatcaggtt taaaagccaa acctaggcaa gcaattttga tttggtcgat
      361 agattttcct gttttttgca aagcttcaat tactgcttca tttacttttg aaataaccca
      421 ttcaggttta tagtcattta cttcacgagc agtacgaata atttttgcca agtcaggagt
      481 tttattcaca ataaaccaag gatctacagc aatgcagtgt ccacccacac cacaacccgg
      541 ttgtaagata ttgactcgtg ggtgtctgtt agccaaagca attagttccc atacattaat
      601 atccaggcga tcacaaataa tagaaagctc atttgcaaaa gcaatattga catcacggaa
      661 actattttcg gtcaatttgc acatttctgc ggtacgagca ttggtaacta tacaatcgcc
      721 ttttacaaaa gttttgtata aatcgaccgc ttgtttagag catttatttg tcataccacc
      781 cacaatacgg tcattttcaa tcaattcacg tatcacttgc cctggtagca ctcgttctgg
      841 gcaatgggcg atacgaatat cagaatcttc acctgcttgc tgagggaagc ttagctctgg
      901 acggtactcg gcaagccact cagccatctg ttctgttgcg ccaaccggag aggtggattc
      961 taaaatgact aaattatctt tttccagcac aggtgcaata gctttacaag cagcttgaat
     1021 ataacttaaa tcaggctcat aatcctcgcc tttaaaaggc gttggtacag caattaaaaa
     1081 tgcttctgct ggttcaggtg tcagtgtggc ttttaatttt ccgctagata cacattggtg
     1141 aacagcaaca tctaaatcag gctcgacaat gtgaattttc ccttgattga tagtgtctac
     1201 ggcgagctga ttaacatcta cgccaattac atttagaccg tgttgggcaa atacagtggc
     1261 ggttggcaag ccaatatagc ctaagcctac tactgaaatg gtattaaagt tcgacataca
     1321 tttattcctt attattttga ttgaaatact gagagaattt gtttcacagc tgtgccatcg
     1381 ccataagggt tgtgagcctc agacattttt gtataagcag cttgatcatt gagtaactca
     1441 gtaacttgtt taataatttc agattgtgca gttccaacaa gttttactgt tccggcttct
     1501 acagcttccg gtctttctgt tgtgtctctc attactaata ccggttttcc taatgatggg
     1561 gcttcttctt gaatacctcc ggagtcagtt aagataaggt aagactctct cattaaataa
     1621 acaaaggaga gatagtcgca aggctcaatg agatgcacat tggatttatc cgctaatagg
     1681 cgatttaccg gttctcttac atttgggttg agatgcacag ggtagataaa ttgaacgtca
     1741 gggaaatcag acgctaatgt tgaaacggct ttacagatgt tctcaaaacc attaccaaaa
     1801 ttttcgcggc gatgtccagt aatcaaaata gtacgcttac tttttatgta agaaaatttc
     1861 tcttggagtg aacttaataa atcaacattt tcatctaatt ttttacaagc gaagaataag
     1921 gcatcaatta cagtattacc cgttacataa atggtgtcct cagtaatatt ttcgttgagt
     1981 agatttttct tggtgctttg tgttggtgca aaatggtatt ttgccaaagc ccctgttagc
     2041 ttgcgattac cttcttctgg ccaaggggag tagatattat gcgttcttaa acccgcctca
     2101 atatgagcga tatcaatttg gttgtagtat gcagccagtg aagtggcaag ggttgttgca
     2161 gtatcgccat gaacaaaaat agtagttggt ttgtattcgg caataacagg ttgaattttt
     2221 tctaaaatag cagaagttac agtagaaagt gtttgcttat tgctcataat atccaggtca
     2281 taatctggag taatttcgaa taattctaaa acttggtcca gcatttggcg gtgctgagca
     2341 gtaacacaga ctttaaaatt tagaccgttt ttcttaaagc cctcgactaa gggagccatt
     2401 ttgattgcct caggacgagt accgaagacg atgagatagc gcatttatta ctcctttaaa
     2461 aatatagata aaactaaaat acactagatg taagtaattt tgttaaatta gttataaaga
     2521 tcgtgttcat tataatgttt ttatggagtt aggtgtataa aaacttttac tttgtagata
     2581 ttttatgtaa attcttgtaa gtatccggta ggttagaaat tatcatgtta tctgctatgg
     2641 tacaatacat aaatacgaat atgagattaa ttttttatgc ttagctattt agtgtttttt
     2701 agtaaaatta taaaaatccc taatttaaaa ggttttttac ctcattgtga aattaggcga
     2761 agacctcttg tgggaaaaac ctttattgct caaaaaagac cgaaccaaat catcggctgg
     2821 gggcttcgtc cttcagcggt gggagcaaga gcttatgcac aaaaacatca acttccctat
     2881 attgcattag aagacggctt tttacgctca ctagggctgg gtgtaaaagg ctatccgcca
     2941 ttttctttag tttatgatga tataggtatt tattacgata ccacctgtcc ttctcgttta
     3001 gaaaagttat tgcttgaaaa acctttacag gatcaagagc aaatcaagct tgcagagaga
     3061 gcattatctc aaatcttaac ttacggactt tctaaatata atcacgcact agattttagt
     3121 gaaatcccct ctgccaaaga aaaaatcgtg ttagttattg accaaacctt tggtgatatg
     3181 gcggtgaaat atggtcaagc gagtgaagtg gatttcaata aaatgttgga aacggcaatc
     3241 agtgagaatc cagaggctca aatttgggta aaaactcacc ccgatgtatt aagtggcaag
     3301 aagaaaggct atttgaccga tttacctcaa tatactgata gggtcagaat tattactgaa
     3361 gatgtgaatc cggcaagttt gattaaattg tgtgagaaag tctattgcgt aacttcgcaa
     3421 atggggtttg aggcattatt attaggtaaa aaagtggtta cctttggtgt accgtggttt
     3481 gccggttggg gggtaacaga tgatcgtaac gaagcggttc aactattagt tcaacaaaat
     3541 agacggaaag atcgttcatt actgcaactt ttttatgctg cgtattttga ttacagtcgt
     3601 tatattaatc cgaatacggg tgaaaaaggc tctatttttg atgtgattga gtatctgcac
     3661 aaggcgacaa tactcaaccg gcgtttaagc ggaaatgtat attgtatagg tatgtcatta
     3721 tggaaacagg ctgtcattaa gccgtttttt aatttgcctc aatgccgttt acattttgtg
     3781 cgttcgctaa attctttaaa aaaacgaccg cttgcagata atgcaaaaat tttagtttgg
     3841 agcaatggca aagcggatgt cttgcaattt gcacagcaga ataatattcc attgttaaga
     3901 atggaagatg gattcattcg ctctgtcgga ctaggcgcta atttagttgc accgctttct
     3961 ttagtagtcg atgatttagg catttatttt aatgcagaga acacttcacg attagaacaa
     4021 attttattga gtgcagagca tttttctgaa actgatttgt cttatgctga aacattaagg
     4081 cagaaattgg tggctagcca tattggaaaa tataatgtgg gcggtggcga tgtaaaattt
     4141 aatgctcaag gtaaaaaaat aattcttgta ccggggcagg tagaagatga cgcttcgatc
     4201 aaatcaggtt ctccacaaat taaaagaaat ctggatttac tgaaaaaagt gagggaatta
     4261 aaccctagtg cctatattat ttataagcct caccctgatg tggtaagtgg taatcgagtc
     4321 ggtcagattc ctacggcaga aacacgcagg tttgcggatc agatggtgga taatgccaat
     4381 attttagatt gtattttaat tgcagatgaa gtgcatacaa tgacatcttt agccggtttc
     4441 gaagcattat taagaggtaa aaaagtgcat tgctacggat taccttttta tgctggttgg
     4501 ggattgacgg aagatagatt gaagtcggca cgcagaggta aatctttgac attatctcag
     4561 ttaattgccg caacattgat atattaccct ctgtatgttg atcctataag caggcagttt
     4621 attgatgcca atcaagcgat tcatatattg tcacaacaga aacgaaacct aaccaatcaa
     4681 aggattaaac gttcttgggt gagtaaacaa tatggaaaat tacagcatct ttatcgtgcg
     4741 tttaaatatg cttcccacac taaataagcg tagagggtag gaaattaaga tggaacatta
     4801 tttagatgag ctaatcatca atagccagag aattttattg ttacaaggcc cgataggctc
     4861 ttttttcact gagtttgccc gttggttaga aagtgagcaa aaagaggtct tcaagttaaa
     4921 ttttaatggc ggagatgaat atttctatcc aaataatagc ccgaatactt ttccttacta
     4981 tgatagctta gataattttg aagcctattt aattcagttt tgccaaactc atcaaataga
     5041 taatatctta tgttttggcg ataatcgtta ttatcataaa gtggctaaaa acgtttgctt
     5101 gaagttaaag ttggtctttt gggcgtttga agaagggtac tttaggccag aatatattac
     5161 gttggaaaaa tggggtgtaa atgctttttc tccaatccct aaaaatcaca ctttcttttt
     5221 gccttatgcc gagttaccag tagtcagtga accagaaaaa gtatctaaag gctttagatg
     5281 tatggcggga aaagcaatcc cgtactattg gaatgcctat aaaaaacgta aagcatacgc
     5341 aaaatatcag catcatcgtt ttctgaatct caaatactac cttaatttat ggacaaggtc
     5401 gggaattaag cgaatatgtt attggttaca tgataaaggc tttgccaagc gggtagaaaa
     5461 aggagagttt ggtgaatttt tcattgtgcc tctacaagtt tatgatgaca ctcaagtgag
     5521 gattcatagt gaacaaaaaa gtgtagagcg ttttttaagg gaagttctga attcctttgc
     5581 caaatttgca ccggcacatc tcaatttaat tattaagcat cacccgatgg atagaggatt
     5641 tatagattat aaaatggtga tttatgaatt cttagagcaa tatccccaat taaacggtag
     5701 agttttttat attcacgatg tgccgttacc tgtcttttta cgttatggga aaggaatggt
     5761 gactttaaat tcaaccagtg ggctttctgc attgctccat aatatgccgg tcaaaacctt
     5821 aggtagagcc aattatgatt ttgagggatt aaccgataaa cagccattag atactttttg
     5881 ggctaatcct cagccaccta attcggaagt atttaaaggc tacagacaat ttcatttaca
     5941 taaaacgcat attaacggta atttttacca taaatcgatt ttgcgttatc cttataattg
     6001 ggaaaatgaa gca
//
LOCUS       DANLXF010000143         5955 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.143, whole genome shotgun sequence.
ACCESSION   DANLXF010000143 DANLXF010000000
VERSION     DANLXF010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5955
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.143"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(157..1443)
                     /gene="purD"
                     /locus_tag="PXD66_001783"
     CDS             complement(157..1443)
                     /gene="purD"
                     /locus_tag="PXD66_001783"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012771675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDL6320062.1"
                     /translation="MNILIIGNGGREHALAWKVRQSPLADKVFVAPGNAGTAMENGIE
                     NVAISATDVPALVTFAKQNSVGLTIVGPEAPLVVGVVDAFRAHGLTIFGPTQAAAQLE
                     GSKAFTKDFLARHQIPTAEYQNFTEIEPALAYLKQKGAPIVIKADGLAAGKGVIVAMT
                     LQEAEDAVRDMLSGNAFGEAGSRVVIEEFLDGEEASFIVMVDGKNVEPMATSQDHKRV
                     GEGDSGLNTGGMGAYSPAPVVTKAIHQRVMDEIIYPTVQGMASENTPYTGFLYAGLMI
                     MPNGQPKVIEFNCRFGDPETQPIMMRLESDLVELCLKACEGKLDSVQSKWCEQAALGI
                     VLAAAGYPGDYRKGDEIIGIPTSTSNQKVFLAGVEQKEGKLLTNGGRVLCATALGNSV
                     FEAQQQALQLAEQIQWQGKFYRRDIGYRAVARKQAK"
     gene            1677..2051
                     /gene="rpsF"
                     /locus_tag="PXD66_001784"
     CDS             1677..2051
                     /gene="rpsF"
                     /locus_tag="PXD66_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDL6320063.1"
                     /translation="MRHYEIVFMVHPDQSEQVPAMIERYTASVKEAGGQVHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQSVIDELETNFRYNDAVLRNLIVHTKAAVTEASPM
                     VKAKESKVAEAVAEVESEEAGE"
     gene            2026..2400
                     /gene="priB"
                     /locus_tag="PXD66_001785"
     CDS             2026..2400
                     /gene="priB"
                     /locus_tag="PXD66_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HDL6320064.1"
                     /translation="MNQRKQASNLPIDNCLILSGTVSSLVKQSLNPLGVPHYSFWLEH
                     RSIQTEVNLERQAWCKIQVVLAGNQFSYLTQQIKLGESVRIKGFIHTHKDFNGLNQLV
                     LHTEQIEFIDQEKPNGTLFPSS"
     gene            2375..2602
                     /gene="rpsR"
                     /locus_tag="PXD66_001786"
     CDS             2375..2602
                     /gene="rpsR"
                     /locus_tag="PXD66_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDL6320065.1"
                     /translation="MARYFRRRKFCRFTAENVVEIDYKDIATLKNYISESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLALLPYTDNHQ"
     gene            2618..3067
                     /gene="rplI"
                     /locus_tag="PXD66_001787"
     CDS             2618..3067
                     /gene="rplI"
                     /locus_tag="PXD66_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDL6320066.1"
                     /translation="MQVILLDKVAHLGSVGDQVTVKSGFARNFLIPQGKAVMATAANI
                     AHFEARRAELEAKAAAALATAQERAAKIAALAAVSVSATAGDDGRLFGSVSAKDIAEA
                     LTAAGVGVAKSEVRLGEGPLRTTGEHQVKIHLHPEVNATVAVNVVAE"
     gene            complement(3131..3907)
                     /gene="dnaQ"
                     /locus_tag="PXD66_001788"
     CDS             complement(3131..3907)
                     /gene="dnaQ"
                     /locus_tag="PXD66_001788"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HDL6320067.1"
                     /translation="MTEEKIIRQVVLDTETTGMNMAGGPPQIGHNIIEIGAVEVINRR
                     LTGRTYHVYIKPPREVDEEAIAVHGITNEFLQDKPVFAEIAEEFLDFIKGAELIIHNA
                     PFDVAFMDQEFSYLPNPPAKTAEMCTVTDSLQMARRMYPGKRNNLDALCDRLGIDNSK
                     RVLHGALLDAEILADVFLMMTGGQISLLGEEETEVVSEVIDIEEEFSAIVLNADEAII
                     LKANDEEEESHLALLKLIEKKSKGNCLWTKALTDTAEISH"
     gene            complement(3995..5665)
                     /gene="ettA"
                     /locus_tag="PXD66_001789"
     CDS             complement(3995..5665)
                     /gene="ettA"
                     /locus_tag="PXD66_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HDL6320068.1"
                     /translation="MSTQFVYTMHRVGKVVPPKRHILKDISLSFFPGAKIGVLGLNGA
                     GKSTLLRIMAGVDKEFEGEARPQPGIKIGYLPQEPKLAPQQTVREAVEEAVAEVKNAL
                     TQLDEVYAMYADENADFDKLAAKQAELEAIIQAHDGHNLQNQLERAADALRLPDWDAK
                     IEHLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDYEGTVVAITH
                     DRYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKEKRLAQEQAAESARQKSIEKELEW
                     VRQNPKGRQAKSKARMARFEELNSGEYQKRNETNELFIPPGPRLGDKVIEVQNLTKSY
                     GDRTLIDDLSFAIPKGAIVGIIGANGAGKSTLFRMLSGKEQPDSGSIVLGETVVMASV
                     DQFRDAMDDKKTVWEEVSNGQDILTIGNFEIPSRAYVGRFNFKGVDQQKRVGELSGGE
                     RGRLHLAKLLQRGGNVLLLDEPTNDLDVETLRALENAILEFPGCAMVISHDRWFLDRI
                     ATHILDYGDEGKVTFYEGNFSDYEEWKKKTFGEAATQPHRVKYKRIAK"
BASE COUNT         1566 a         1384 c         1268 g         1737 t
ORIGIN      
        1 tccacatata gggcacgcag agtcatttag aaaagagttt actgcatcgt ttaatgatag
       61 tgatttaatt tcggggcctg catcgagttc agtgatatta atttcattaa gtgttgtact
      121 cataataaat ttttccttgt tttaagagtt tataaattat ttcgcttgtt ttcttgccac
      181 tgctcggtag ccaatatctc ggcgatagaa tttgccttgc cattgaattt gttcggcaag
      241 ttgtaaggct tgttgttgag cttcaaatac actgttgcca agtgcggtgg cacaaagtac
      301 acgtccaccg ttggtgagca gttttccttc tttttgctca acccctgcta agaaaacttt
      361 ttgattggag gtagaggtcg gaatgcctat gatttcatcg cctttacggt aatcgcccgg
      421 gtagcctgcg gcagctaaaa caatgcctaa tgcggcttgt tcacaccatt ttgattgcac
      481 gctatccaat ttgccttcgc acgcttttaa gcacaattca acgaggtctg attccaaacg
      541 catcataatc ggctgagttt cagggtctcc aaaacggcag ttaaattcaa tcactttcgg
      601 ctgaccgttt ggcataatca ttaagccggc atagagaaaa ccggtgtaag gggtattttc
      661 gcttgccatt ccttgcacgg tcgggtaaat aatttcgtcc attacacgtt ggtggattgc
      721 ctttgttacc accggagcag gggagtaagc ccccattccg ccggtattta agccgctatc
      781 gccctcgcca acacgtttgt ggtcttgtga ggttgccatt ggttcgacat ttttgccgtc
      841 caccatcaca ataaaactgg cctcttctcc gtctaaaaat tcctcgatca ccacacggct
      901 gccggcttca ccaaaggcgt tgcctgacag catatcacgc actgcatctt ccgcctcttg
      961 taaagtcatt gcaacaatta cgcctttgcc tgcggctaaa ccgtctgctt tgattacaat
     1021 cggtgcacct ttttgtttta agtaggcaag tgccggttca atttcggtga aattttggta
     1081 ttctgcggtt ggaatctgat ggcgagccaa gaaatctttg gtaaatgctt tagaaccttc
     1141 taattgagca gcggcttggg tcgggccgaa aatggttaaa ccgtgggctc gaaaagcatc
     1201 aaccacgcca actactaacg gtgcttcagg gccgacaatc gtcaaaccta cgctattttg
     1261 tttagcgaat gtaactaagg caggcacgtc agtggcggaa atagctacat tttcaatgcc
     1321 gttttccatc gctgtgccgg cattgccggg ggcgacaaag actttgtctg cgagcggcga
     1381 ttgacgaact ttccaagcaa gagcgtgttc acgcccaccg ttgccgataa ttaaaatatt
     1441 cattgggatt ccttgcaaaa atgataaagc aaacgtttgc gtattgtact tgatttgttt
     1501 atctgctgac aagcagtcgt ttttctcgga ttttttgcaa aatacgaaat tgttgcttgc
     1561 aaaataggct tttttcagta taatcctgcc gttcagtcac attcgttttt taatccttgc
     1621 ttccacaagt tggtgactgg ctaaagttgg aggctgctaa cccgtaagga tagtcaatgc
     1681 gtcactacga aatcgttttt atggttcacc cggaccaaag cgaacaagta ccagcaatga
     1741 ttgagcgcta cacagcgtct gtaaaagaag ctggcggtca agttcatcgc ttagaagatt
     1801 ggggtcgtcg tcaattagca tacccaatca acaaacttca caaagcacac tatgtgttaa
     1861 tgaatgtaga agcacctcaa agtgtaatcg acgagttaga aactaacttc cgttataacg
     1921 atgcagttct tcgtaactta atcgttcaca ctaaagcggc agtaactgaa gcgtcaccaa
     1981 tggtgaaagc gaaagaaagc aaagttgctg aagcggtagc tgaagttgaa tcagaggaag
     2041 caggcgagta atttgccgat tgataattgt ttaatcttaa gcggaacggt ttccagcttg
     2101 gttaaacaaa gcctaaaccc ccttggtgtg cctcattaca gtttttggtt agaacatcgt
     2161 tcaatccaaa ccgaagtgaa tttagaacgc caagcgtggt gcaaaatcca agtggttttg
     2221 gcaggcaatc aatttagtta tttaacccaa caaattaagc tcggtgagag cgtgagaatt
     2281 aaaggtttta tccatactca caaagacttt aacggtttaa atcagttagt cttacacacc
     2341 gaacaaattg aatttataga tcaggagaag ccaaatggca cgttatttcc gtcgtcgtaa
     2401 gttctgccgt ttcacagcgg aaaatgttgt tgaaatcgat tacaaagata tcgctacatt
     2461 aaagaactac atttctgaaa gcggcaagat tgttcctagc cgtattactg gtactcgtgc
     2521 gaagtatcaa cgtcaattag ctcgtgcaat taaacgcgct cgctaccttg cgttacttcc
     2581 atatactgat aaccaccaat aatcaggaga gagtacaatg caagttattt tattagacaa
     2641 agttgctcac ttaggttctg tgggcgacca agtaaccgtt aaatctggtt ttgctcgtaa
     2701 cttcttaatc ccacaaggta aagcggttat ggcaactgcg gcaaacattg ctcactttga
     2761 agcacgtcgt gcagaattag aagcaaaagc tgcagcagca ttagcaactg cacaagaacg
     2821 tgcagcgaaa attgcagcat tagcagcagt atcagtatct gcaactgcag gtgatgatgg
     2881 tcgtttattc ggttctgtat cagctaaaga catcgctgaa gcattaactg cagcaggtgt
     2941 tggagttgct aaatctgaag ttcgtttagg tgaaggtcca cttcgtacaa ccggcgaaca
     3001 ccaagttaaa attcacttac accctgaagt gaatgcaact gtagcagtaa acgttgttgc
     3061 tgaataatca gtagaaatag aaaacccagc gaaagctggg ttttttgttt gctttattta
     3121 ttggttagaa tcagtgagat atttctgctg tgtcggttaa tgcttttgtc cataggcaat
     3181 ttcctttgga ttttttctca atcagtttca gaagtgctaa atgcgactct tcctcttcat
     3241 cgtttgcttt aagaataatt gcctcatcgg catttaacac aattgcagaa aattcttctt
     3301 caatatcaat aacttcactt accacttcag tttcttcttc gcctaataat gaaatctgac
     3361 cgccggtcat cattaggaat acatcagcta agatctccgc atccagtaac gccccgtgta
     3421 gtacacgctt gctattatca atgccgagcc tgtcgcaaag tgcatctaaa ttattccgtt
     3481 tgccggggta cattcttcgt gccatttgca ggctatcggt tacggtacac atttctgcgg
     3541 tttttgccgg tggattaggc agataagaaa attcttgatc cataaacgcc acatcgaagg
     3601 gggcattgtg aataattaat tccgcacctt tgatgaaatc caaaaactcc tcggcaattt
     3661 cagcaaaaac aggtttatct tgcaaaaatt cattggtgat gccgtgaact gcaatcgcct
     3721 cttcatctac ctcacgcggt ggcttgatat aaacgtgata agttctgccg gttaaacggc
     3781 ggttaatgac ttcaaccgca ccgatctcaa taatattatg cccaatctgt ggcgggccac
     3841 ctgccatatt cattcctgtg gtttcagtat ccagcactac ttggcggata attttttctt
     3901 ctgtcatatt caatttaaaa ttgtaggggt gaaatatttt tatcccttaa ttctgcattt
     3961 gattaggggc gaaacatatt tcgcccctac attgttactt cgcaatccgt ttatatttca
     4021 ctcggtgcgg ttgggtggcg gcttcaccga aggttttctt cttccactct tcatagtcgg
     4081 agaagttacc ctcgtagaac gtcactttgc cttcatcgcc gtagtctaaa atatgggtgg
     4141 cgatacggtc taagaaccag cggtcgtgcg aaattaccat tgcacagccc gggaactcta
     4201 agatcgcatt ttccaacgca cgcagggttt ctacgtctaa gtcgttggtc ggctcgtcca
     4261 gtaacaacac gttaccgcca cgttgtagta gcttcgccaa gtgtaaacga ccacgttcac
     4321 cgcctgaaag ctcgcccaca cgtttttgct gatctacgcc tttgaagttg aaacgcccca
     4381 cataagcacg gcttgggatt tcaaagttac caatcgtgag aatatcctgc ccgttcgaca
     4441 cctcttccca cacggttttc ttatcgtcca tcgcatcacg gaactgatcg accgatgcca
     4501 ttaccaccgt ttcaccaagc acgattgaac cagaatccgg ctgttcttta ccagaaagca
     4561 tacggaacaa ggttgattta cccgcaccat ttgccccgat aatcccgaca atcgccccct
     4621 tcggaatagc gaacgacaag tcgtcaatca aagtgcgatc gccgtaagat ttagtcaggt
     4681 tttgtacctc gatcacttta tcacctaagc gtggacccgg tggaataaag agttcgttag
     4741 tctcgttacg tttttgatat tcgcctgagt taagctcttc aaagcgtgcc atacgagcct
     4801 tacttttcgc ttggcgacct tttggatttt ggcgtaccca ttccaactct ttctcaatcg
     4861 atttttgacg agccgattcc gccgcttgct cttgtgccaa gcgtttttct ttctgctcca
     4921 accaagaaga gtagttacct tcccaaggaa tgccttcacc acggtcaagc tctaagatcc
     4981 agcccgcaac gttgtctaag aagtaacggt cgtgggtaat tgccactacg gtgccttcgt
     5041 agtcgtggag gaaacgctcc aaccacgcca ccgattccgc atctaagtgg ttggtcggct
     5101 catctaacaa taacatatcc ggtttttcca gcaacagacg gcaaagtgcc acacggcggc
     5161 gttcaccccc tgataaatgc tcgatttttg cgtcccaatc cggcagacgt aacgcatcgg
     5221 cagcacgctc taactggttt tgaaggttat ggccatcgtg ggcttgaata atcgcctcta
     5281 attccgcctg ttttgccgct aatttgtcga aatcggcatt ctcatcagca tacatcgcat
     5341 agacttcatc aagctgggtt aaggcgtttt tcacttccgc aaccgcttct tccaccgctt
     5401 cacgcacggt ttgctgtggg gcaagtttcg gttcttgtgg aagatagccg attttaattc
     5461 ccggttgtgg acgagcctcg ccctcgaact ctttatctac gcccgccata atgcgtaaaa
     5521 gcgttgattt acccgccccg tttaagccga gcaccccgat tttcgccccc gggaaaaagc
     5581 tcagggaaat atctttaaga atgtgacgct tcggcggcac gactttgccg acacggtgca
     5641 tcgtatatac aaattgagtt gacattggtt gtattcctca caaaaaaatt cgccttattt
     5701 tacttgaatt tacagccaaa atatagggtg gttttggctt ttggtaaagt aaccttttcc
     5761 aatagagatg ttatttgagg ctgcatagta ctatgcattt ctcttacctt gacaggtaag
     5821 agaaacaaat cctgaaggaa aaaaatttta aggaagttat tctatgcaat atgtatgtcc
     5881 aaaaacactc aaattattca acagcattgt tggttgttat aagggagaag attaattctc
     5941 tacctcatca aggct
//
LOCUS       DANLXF010000144         5924 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.144, whole genome shotgun sequence.
ACCESSION   DANLXF010000144 DANLXF010000000
VERSION     DANLXF010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5924
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.144"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            11..781
                     /locus_tag="PXD66_001790"
     CDS             11..781
                     /locus_tag="PXD66_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EfeM/EfeO family lipoprotein"
                     /protein_id="HDL6320069.1"
                     /translation="MATQAAALDLTKETEAYKQFVVEQIDQLVTDTEKFVGYLNKGDV
                     QKAKKIYPLARMYFERSEPIAESFGDLDPRIDARLADLAEEGKTEKDWSGFHKIEKVL
                     WQKNTTKGTKATAEQLLKDVKELRAKIPTAEVTPEPMITGAVDLLNEVSTTKVTGEEE
                     IFSKTDLYDFKANIEGAEKIYEIFKTQLEQIDAKLSTEIGSRFAAVNALLAKHNKSKT
                     GYDYVPYNKLSKADIKALAEAVNQLGEPLAQLGVLLNK"
     gene            792..1988
                     /gene="efeB"
                     /locus_tag="PXD66_001791"
     CDS             792..1988
                     /gene="efeB"
                     /locus_tag="PXD66_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HDL6320070.1"
                     /translation="MMAETTSRRDFLKHTALVGAGLLSAPAFALESRQISEKSQNQYD
                     FYGKHQAGIVSPAQKHIYFLVLDLDTTDIAKVKTLFQNWTEYSRNLTLGKNVKPYSAN
                     AYVPPADTGEADSLSAQNLTLTFGVSANFFTKLGIEKHKPKELNDLPHFPRDQLQAEY
                     TGGDICIQACADDPQVAFHAVRNLVRVARGEIKMKWSQMGFNSFANQDTPRNLFGFKD
                     GTANAESLKDQENTVWVQQEGWLKGGSYLAVRRVKMFLETWDRTHLQSQEETFGRHRD
                     SGAGIGHQHEFDKLDLAKKDEKGNAVIPEVSHAHLANKTGLKILRRSFSYSSGIDDKG
                     QFDSGLLFVSFQQSPEQFIKIQNALGNVDKMNEYITHIGSGLFACFAGVKDENDYLGK
                     ALFDLI"
     gene            1999..2618
                     /locus_tag="PXD66_001792"
                     /pseudo
     CDS             1999..2618
                     /locus_tag="PXD66_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596976.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+/Pb2+ permease"
     gene            complement(2654..3472)
                     /locus_tag="PXD66_001793"
     CDS             complement(2654..3472)
                     /locus_tag="PXD66_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDL6320071.1"
                     /translation="MKYDLHAHSTASDGVLSPTELVQRAVEQGVTMLALTDHDTISGI
                     QEAKNFAQNQPLELISGVEISILWQEKSIHLAALNIDENNEELVKFLDNQAQLREERA
                     VEIGEKLAKVGIANAYEGAKALATGEVTRAHYGRFLYEQGYVRNIEHAFKRYLGMGKP
                     AYVKPRWSSLEDAVNVTHTSGGVISIAHPLRYKMTARWIRRLIADFKQAGGDGIEVSG
                     CGQTPDQRQLLARWAKEFDLYGSVGSDFHYPTGWIELGKSLELPQECKPIWTLF"
     gene            complement(3484..4083)
                     /locus_tag="PXD66_001794"
     CDS             complement(3484..4083)
                     /locus_tag="PXD66_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HDL6320072.1"
                     /translation="MTMDNLPLEQHPFDPILPEQATVVMVGTFPPTSEKRCMEFHYPN
                     FQNDMWRIMGNIFYADPNYFRVGEEKRFDPIRIEAFLREKGVALCSSAKTAIRLKGNA
                     SDKDLKIVDPIDMDELLLKLPKLKWLFTTGGLATDTLLGLLPEKHKAPKTNEWVVYPY
                     SATRELYLYRLPSTSRAYPLSLANKTEAYRQFFRTAGLI"
     gene            complement(4080..5627)
                     /gene="sbcB"
                     /locus_tag="PXD66_001795"
     CDS             complement(4080..5627)
                     /gene="sbcB"
                     /locus_tag="PXD66_001795"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HDL6320073.1"
                     /translation="MSDKFSFFFYDYESFGVSPALDRPAQFAGIRTDADFNIIGEPVM
                     FFCKQTPDYLPSPEAVMVTGITPQQCNAEGVSEPEFAARIHAEFSRANTCTIGYNNIR
                     YDDEMTRYTFFRNFYDPYEYSWKNGNSRWDLLDLVRACYALRPDGIEWPTDETGMPSF
                     KLENLTKANGIAHENAHDAMSDVYATIAMAKLIKQKQPKLFDFFFNLRSKKEVEKLID
                     TGEMTPLVHVSGMLGNYRGNTAWVVPLAWHSTNQNAVIVCDLSGDMNGLLTENAEVLR
                     QRLYTKRDELAENELPVPLKLVHINKCPILAPAKTLLPENAERLGIDRALCLENLKTL
                     KAQKSLIREKVIEIFNEERTFEPSTNVETTLYDGFFSPADKNNMAILRTLPPEDLANH
                     GLKFEDPRVEALLFHYRARHYPQTLSRAEQIKWQKHCNQQIEAKAAQFAQSIDDLFQQ
                     HHDNPEKVKLLENLTAYAEQISQQQAVIYRQNVAKDEKLLSELNRVAEQPLDKTEKLK
                     MLKELIK"
BASE COUNT         1783 a         1251 c         1220 g         1670 t
ORIGIN      
        1 tggctttttt gtggctactc aagcggcagc tttagactta actaaggaaa ccgaggctta
       61 taaacaattt gtggtagaac agattgacca actggttacc gatacggaaa aatttgtcgg
      121 ctacttaaac aaaggcgatg tgcaaaaagc gaagaaaatt tacccgcttg caagaatgta
      181 ttttgaacgt tctgaaccga ttgcagaaag ttttggcgat ttagaccctc gtattgatgc
      241 ccgtctggca gatttagccg aagaaggtaa aaccgagaaa gattggtcag gtttccataa
      301 aatcgagaaa gtattgtggc agaaaaacac cactaaaggc acaaaagcaa cggcagaaca
      361 attattaaaa gatgtaaaag agctgcgtgc gaaaattccc actgcagaag ttacccctga
      421 gccgatgatt accggtgcgg tagatttatt aaacgaagtt tctactacta aagtaaccgg
      481 tgaagaagaa attttctcta aaaccgacct ttacgatttt aaagccaata ttgaaggtgc
      541 agaaaaaatc tatgagattt ttaaaacgca attagagcaa attgatgcca aactctcaac
      601 agaaatcggc tctcgttttg cggctgtcaa tgcgctgtta gcaaaacaca ataaatctaa
      661 aaccggttat gattatgtgc cttataacaa actgtcaaaa gcggatatta aagcgttagc
      721 agaggctgta aaccaattag gcgagccgtt agctcaactt ggcgtacttt taaataaata
      781 gtggagcaaa aatgatggca gaaaccactt ctcgccgtga ttttttaaaa cataccgcat
      841 tagtaggagc aggcttattg tctgctcctg ctttcgcttt ggaatcacga caaatatctg
      901 aaaaatctca aaatcaatac gacttttacg gcaaacatca agctggtatt gttagccctg
      961 cacaaaaaca tatctacttt ttagtgctgg atttagacac cactgacatt gccaaagtaa
     1021 aaacattgtt tcaaaactgg acggaatata gccgtaacct cacgcttggc aaaaatgtga
     1081 aaccttatag tgctaatgct tatgttccac cggcggatac cggtgaggca gatagccttt
     1141 cggcacagaa tctcactctt acttttggcg tcagtgccaa tttctttaca aagctcggca
     1201 ttgaaaaaca taagccgaaa gaactgaatg atttaccgca tttccctcgt gatcaactac
     1261 aagcagaata caccggtggc gatatttgca ttcaagcctg tgcagacgat ccgcaagtgg
     1321 ctttccacgc tgtgcgtaat ttggtgcgtg ttgcccgtgg tgaaatcaaa atgaaatggt
     1381 cgcaaatggg gtttaactcc tttgccaatc aagacacacc tcgtaactta ttcggtttta
     1441 aagacggcac tgccaacgca gaaagtttaa aagatcagga aaatacggta tgggtgcaac
     1501 aagagggctg gctcaaaggc ggttcttatt tagccgttcg ccgtgttaaa atgttcttgg
     1561 aaacttggga tcgcacccat ctccaatccc aagaagaaac cttcggtcgt caccgtgatt
     1621 ccggagccgg aatcgggcat cagcacgaat ttgataaatt agatttagcc aaaaaagacg
     1681 aaaaaggcaa tgcagtaatt ccggaagttt cacacgctca tcttgccaat aaaacggggc
     1741 tgaaaatcct acgccgttca ttctcctatt caagcggtat tgatgataaa gggcaatttg
     1801 attcagggtt gttgtttgtc tctttccaac aaagtccgga gcaatttatc aaaatccaga
     1861 acgctctcgg caatgtggat aaaatgaatg aatacatcac tcatatcggc agtggtttat
     1921 ttgcctgttt tgccggtgtg aaagatgaaa atgactacct tggcaaagcg ttgtttgatt
     1981 taatttaggg aaaaggaaat gaaattaaaa gcattagtgc taattccgtt actcttttcg
     2041 agtacaggat tgcttgccaa agtgaatgtc agccccttat ttgttcagct ttccgatgca
     2101 atggcagaga gtaaaaaagg ggaatttgca aaatccagcc aaaatttgac cgctttacag
     2161 cacgattttg agcagattag ccagcacgat tccctcgccg gagttgaagt acagaccgct
     2221 ctgaaaaaag cgattgaaac acccaatgct gaaaacttag agagcctttc taaaaactct
     2281 atgcgtttga aaaagaacaa aaccctagcg gttatgctga aaaacatcag gattttgtgg
     2341 caaaaatgac gccgctttat gcagaacttg ctcaaaccgt gccaactcaa gaaatcgcca
     2401 aaatcaaatg ggcggcttat cagtttggta agaattgggt aaaacaggaa aaagcagtac
     2461 gggaaatcag tcttccccat tatggcaaat ttgagcgtat tcttggttta atgcgaatta
     2521 atctgaacgc tgaaaagccg gatatggcaa aaatcagtga actggtttcc gaactaggtg
     2581 tggtaatggc agactttaag caagttaaag tgaaatagta aaaaatcggg gcaaattatt
     2641 tgccccgata acactaaaat aacgtccaaa tcggtttaca ttcttggggt aattccaaac
     2701 ttttaccgag ctcaatccag ccagttgggt agtggaaatc agaaccgacc gagccatata
     2761 aatcaaactc ttttgcccaa cgggcaagta attgccgttg atcaggtgtt tgtccgcagc
     2821 ctgatacttc aatgccatcg cctccggctt gtttgaaatc tgcgattaaa cggcgaatcc
     2881 aacgagcagt cattttatag cgtagcgggt gagcaatcga aatcacaccg cctgaggtgt
     2941 gggtaacatt caccgcgtct tctaaactac tccatctcgg tttcacatag gcaggtttcc
     3001 ccattcccag ataacgctta aaggcgtgtt caatattgcg gacatagcct tgttcataca
     3061 aaaaacgccc ataatgtgcc cgagttactt cccctgttgc caacgctttt gccccctcat
     3121 aggcatttgc aatcccgact tttgctaatt tttcaccaat ttcgaccgct ctctcttccc
     3181 ttaattgggc ttggttatcc aaaaacttca ccagctcctc gttgttttca tcaatattca
     3241 acgccgcaag atgaatactt ttttcttgcc ataaaatcga aatttccacc ccagagataa
     3301 gctcaagcgg ttgattttgg gcaaaatttt tcgcttcttg aatgcccgaa atcgtatcgt
     3361 gatcagttaa agctagcatt gttacccctt gctctaccgc acgttgtacc aattcagtgg
     3421 gagaaagtac gccatcagag gcggtactgt gggcgtgtaa atcatatttc attttattta
     3481 tccttaaatt aagccggcag tcctgaaaaa ttggcgatag gcttcggttt tgttggctaa
     3541 gctgagtgga taagccctcg acgtcgaagg caggcgatat aaataaagtt ctcttgtggc
     3601 ggaataagga tagaccaccc attcgttggt tttcggagct ttgtgttttt ccggcaataa
     3661 accgagcaac gtatcggttg ccaaaccacc tgtggtaaat agccatttca gtttgggtaa
     3721 tttgagcaac agttcgtcca tatcgatagg atcaacaatt tttaaatctt tatctgaagc
     3781 gtttcctttt aaacgaatcg ctgtttttgc cgaagaacag agcgcaacgc ccttttctcg
     3841 caaaaacgcc tcaatccgaa tcggatcaaa gcgtttttct tcaccaactc gaaaatagtt
     3901 agggtcagca taaaaaatat tgcccataat tcgccacata tcattttgaa aattcgggta
     3961 atgaaattcc atacaacgct tttccgatgt aggtggaaaa gtgccgacca ttaccaccgt
     4021 tgcttgttca ggtaaaatag ggtcaaacgg gtgctgttct agcggtaaat tatccattgt
     4081 catttaatca actcttttaa catcttgagc ttttcggttt tatctagggg ctgttccgct
     4141 actctgttaa gctcgctcag caatttttca tcttttgcaa cattttgacg ataaatgacc
     4201 gcttgttgtt gcgaaatctg ctcggcgtaa gcggtcaaat tttctaataa ttttaccttt
     4261 tccgggttgt cgtgatgttg ttggaacaag tcgtcaatcg attgggcgaa ctgcgctgct
     4321 tttgcctcaa tttgctggtt gcaatgtttc tgccatttga tctgctcggc tcgactgagt
     4381 gtttgtggat agtgccttgc tcggtaatgg aatagtaacg cctctactcg agggtcttca
     4441 aatttcaagc cgtggttggc taaatcttca ggcggtagcg tgcgtaaaat cgccatatta
     4501 tttttatccg caggcgagaa aaagccgtca tacaaagtgg tttctacatt ggtcgaaggc
     4561 tcaaacgtgc gttcttcatt aaaaatttca atcacttttt cccgaatcag cgatttctgt
     4621 gcttttaaag ttttcaaatt ttccaaacat aaagctctgt ctatgcctaa acgttctgca
     4681 ttttcaggca gtaaggtttt tgccggtgct aaaatcgggc atttattaat atgcactaat
     4741 tttaacggca caggtaactc attttcggct aattcatctc gtttggtgta taaacgctga
     4801 cgtaaaacct ctgcattttc agtcaataag ccattcatat cgcccgataa atcacacaca
     4861 attacggcat tttgattagt tgagtgccaa gccagtggca caacccaagc ggtatttcct
     4921 cgatagttgc ccagcatacc ggaaacgtgc acaagcggtg tcatttcacc agtgtcaatt
     4981 aacttctcta cttctttttt actgcgtaaa ttaaagaaaa aatcgaacaa ctttggctgt
     5041 ttttgcttaa ttaattttgc cattgcgatg gtggcataca catcagacat cgcatcgtgt
     5101 gcattttcat gggcaatgcc attggctttg gtgaggtttt ccaatttaaa acttggcata
     5161 ccggtttcat ctgttggcca ttcaatacca tcaggtctta acgcataaca ggcacggact
     5221 aaatctaaca agtcccaacg agaatttccg tttttccagc tatattcgta aggatcgtaa
     5281 aaattgcgga agaaggtata acgggtcatc tcatcatcat aacgaatatt gttataaccg
     5341 atagtacaag tattcgctcg gctaaattca gcgtgaattc gggctgcaaa ttccggctca
     5401 gatacacctt cagcgttaca ttgttgcggt gtaatcccgg taaccatcac cgcttcgggg
     5461 ctgggtaaat aatccggcgt ttgcttacaa aaaaacatta ccggttcacc gataatatta
     5521 aaatccgcat cggttcgaat accggcaaat tgtgccggac gatctaacgc cggactcacg
     5581 ccgaaacttt cataatcgta aaagaaaaaa gagaatttat cgctcattcg ttcaatttgt
     5641 tagaaaaaat tgggctgatt gtagcacagt aaggcagaaa ggagggaata caagcggtca
     5701 atttccgtta gaaatttgca aatgctaaaa taaaaaaagc ccctaatcaa aaaagcgaaa
     5761 aggggcggag gtcttaccta aggaaatatg aaaaaaactt gcataaatct gttacatata
     5821 ctcagactgc ctaactgaac gaaagttcat taaaaaataa aaaatttttt aacaccaagc
     5881 aaaatcagat ttaactactt gcaatgctta tatagactca caaa
//
LOCUS       DANLXF010000145         5899 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.145, whole genome shotgun sequence.
ACCESSION   DANLXF010000145 DANLXF010000000
VERSION     DANLXF010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5899
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.145"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            18..875
                     /gene="djlA"
                     /locus_tag="PXD66_001796"
     CDS             18..875
                     /gene="djlA"
                     /locus_tag="PXD66_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HDL6320074.1"
                     /translation="MQFIGKIVGFFLGYQLFHGLFGGILGAFIGHLADKKLYELGSVR
                     SSLFGKNLTRQSLFTQTTFAVLGHIAKAKGRVTEADIELARALMARLKLDSAAQQLAQ
                     NAFTLGKEPDFPLRQVIQEFREACGARTDLLRFFVEVQMQAALQDGQLDVNEQQILFT
                     IAETMGMSRFQFEQMIAMIMAAQQFRNGGYYQQNGSYHQQSQGGYQGSYQQPAQPSIE
                     AAYTVLGVTAQDDQNTVKRAYRKLMNEHHPDKLAAKGLPDEMMELAKEKAQQIQAAYD
                     LICKVKEWK"
     gene            875..1501
                     /locus_tag="PXD66_001797"
     CDS             875..1501
                     /locus_tag="PXD66_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HDL6320075.1"
                     /translation="MDSRSHIIPLFLAIVITVIAVWSGLNPTDRAVWYAEVIPIFIVF
                     ALLIITYPKFQFSGLAYFLMSLWLIMHLIGAKYTFANVPFEWANQFLTPILGEDRNHF
                     DRVAHYIIGFYSFPMAEWLLRKRKCGMGVAFFFSLFFIMSVAAAYEIIEWQYAVIEGG
                     NAGVEFLGSQGDIWDAQKDMLADTLGALTALMIYLIARPDLRIHDRHF"
     gene            1579..2532
                     /gene="dusC"
                     /locus_tag="PXD66_001798"
     CDS             1579..2532
                     /gene="dusC"
                     /locus_tag="PXD66_001798"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HDL6320076.1"
                     /translation="MNPIPNVILAPMQGVLDPFVRNLLTSVNDYDLCISEFVRVVDQR
                     LPKKTFYRLAPELYQGGLTDSGTPVRVQLLGQHPQWLAENAALAIELGSHGVDLNCGC
                     PSKTVNGSNGGASLLKDPELIYRATKAMREAVPSDKIVSVKVRLGWDSVSQCFEIADA
                     VEQGGANEITVHGRTKVDGYRAERINWQAIGEIQQRLSIPVIANGEIWDFESAKNCQK
                     ITACKALMMGRGALNTPNLSKVVKYNVPKMAWNEVLQLLFKYVQMENQFDTGFYHVAR
                     IKQWLRYLDKEYPQAVELFDILKTEHGYDGLKAHIEKAVEE"
     gene            2532..3578
                     /locus_tag="PXD66_001799"
     CDS             2532..3578
                     /locus_tag="PXD66_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HDL6320077.1"
                     /translation="MSKQIFNEEELKTTEHFEPKQEFDLENAIIEEEEKAVEAELIIE
                     ESLKPSRFWIRVLLATLALFGVAVIAQSIQWLIDTFQQREWIYFAFSIVFFIVSLLGV
                     GAIISEWRKLVYLRNHQKNQQMSNQLLLEQLPSNDGEQAVKFCENIANQLNHLPSIAQ
                     AEQLWKSELNEAYNAKEVLYLFSENVLKPIDKQVKQMISRNAAENAVIVAVSPLAIVD
                     VLLMAARNIALVNKITKAYGMELGYISRLKLFKMVLKNMVFAGATEIATDVGMDFFSQ
                     NLTAKLSLRAAQGIGVGLLTARLGIKAMEFCRPVAFQANEKPRISAIRQELLTSVKNT
                     VFAKTETKEKVFSD"
     gene            complement(3615..4274)
                     /locus_tag="PXD66_001800"
     CDS             complement(3615..4274)
                     /locus_tag="PXD66_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL6320078.1"
                     /translation="MAVTIYLVRHGRTVWNLEGRLQGSGDSALVEEGIIGAKKTGIAL
                     KHIPFTAAYSSMQKRAQDTANYILAENERSNIPHFHHKGLNEFDFGSWEGMKSVDLQE
                     NDEYWVMKRTPAEYLAKANGGERFEQLYQRVTQAFNQIAELHQNDGKVLIVSHGMTLT
                     LLTAVLKGIAWQDFRNEEKHSFVFNTAITKVEVENGKVTVLEFNNTDHLDNVPTSQYK
                     R"
     gene            4323..4976
                     /locus_tag="PXD66_001801"
     CDS             4323..4976
                     /locus_tag="PXD66_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HDL6320079.1"
                     /translation="MTPTLPICYQHRDFIIINKPEGLSVHKDEAEIGLTELVARQLNV
                     KQVWLVHRLDKITSGLLILALNKEAAARFYHLFEQHKIYKTYWALSDKKPKKKQGRIV
                     GDMQKSRNGAWKLCHSKENPAVTQFISHSVEDSLRHFILQPKTGKTHQLRVAMKSLGS
                     PILGDKLYSGSQADRVYLHAYQLDFEYENEKISVQALPTSGHFWQKICKSNHIKHGE"
     gene            4978..5598
                     /locus_tag="PXD66_001802"
     CDS             4978..5598
                     /locus_tag="PXD66_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HDL6320080.1"
                     /translation="MKLWYSTTSPFARKALAVIKHHQLDDKVEMLRVTKAMDPNSPHN
                     QDNPLGRIPALQRNCGRWLFGSLLISEYLDQKGNNTPLLPKEGKPRWAVLALHNLADG
                     IMENTMPTLLQERMARPENEWWVSRHEQLMERNIRSFRQLEEALQEFGTELNLGTLTA
                     VALIDWWAFRLDKIGYDLAKNYPNLTAWAEEMNNKYAILAETKPSM"
     gene            complement(5645..5863)
                     /locus_tag="PXD66_001803"
     CDS             complement(5645..5863)
                     /locus_tag="PXD66_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015702330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL6320081.1"
                     /translation="MWLRELFLKRRLELGLTQRTLGEKMGVLYSFIGKVETGDRRLDI
                     FEFIAYCKGLDLDPLSVLQEIEQQFPHK"
BASE COUNT         1736 a         1156 c         1321 g         1686 t
ORIGIN      
        1 tataaaagga taaaaaaatg caatttatcg gaaaaatagt cggttttttc ttgggctatc
       61 agctttttca cggtttattt ggtgggatac ttggcgcttt tatcgggcat ttagccgata
      121 aaaaactcta tgaattaggt tcagttcgtt caagtctttt cggtaaaaat ttaactcgcc
      181 aatcgctttt cacccaaacc acttttgcgg tgttggggca tattgcgaaa gcaaaagggc
      241 gtgttactga agcggatatt gaattagccc gagcgttaat ggctcgctta aaattagata
      301 gtgcagccca gcaattagcg caaaatgcct ttaccttagg taaagagcct gacttcccgt
      361 tacgccaagt cattcaagag ttcagagaag cctgtggggc aagaactgac ttattacgtt
      421 tctttgtcga agtgcaaatg caggcagcct tgcaagatgg gcaacttgat gtgaacgagc
      481 aacaaattct gtttacgatt gccgaaacaa tgggaatgtc tcgcttccaa tttgagcaga
      541 tgatcgcaat gattatggca gctcaacaat tccgcaatgg tggctattat cagcaaaatg
      601 ggagttatca tcagcaatct caaggtggct atcagggcag ttatcagcag cctgcacaac
      661 caagcattga ggcagcctat accgtattag gcgtaaccgc acaagacgat caaaatacag
      721 tgaaacgggc ttatcgtaag ttaatgaacg aacatcaccc ggataaatta gccgctaaag
      781 gtttgcctga tgagatgatg gagttggcaa aagagaaagc tcaacaaatt caagcggcgt
      841 acgatttaat ttgtaaagtg aaagagtgga aataatggat tcacgctcgc atatcatccc
      901 tctattttta gcgattgtta ttaccgtgat tgcggtttgg tcagggttaa atccgacaga
      961 ccgcgcagtt tggtatgcgg aagtgatacc gatttttatc gtttttgccc ttttaattat
     1021 cacttaccct aaatttcagt ttagtgggtt agcttatttt ttgatgtcgc tttggctgat
     1081 tatgcacttg atcggggcaa aatatacctt tgccaatgtg ccgtttgagt gggcaaatca
     1141 atttctaacc ccgattttag gtgaagatcg taaccatttt gatcgagtag cacattacat
     1201 tatcggtttt tacagcttcc cgatggcaga atggttactg cgtaaacgca agtgcggaat
     1261 gggcgtagca tttttcttct cgctgttttt cattatgtcg gtggcggcag cttacgaaat
     1321 tattgaatgg caatatgccg tgattgaagg tggcaatgcc ggcgtggaat ttctaggctc
     1381 gcaaggggat atttgggacg ctcaaaaaga tatgcttgcg gatactcttg gggcattaac
     1441 cgcactgatg atttacctga tcgctcgccc cgatttgcgc attcacgata gacattttta
     1501 accttcaagc ggttgtttct tgccaatttt ttgcaaaatg ttgagaaaaa ataaccgctt
     1561 gtatttttta ttttaccaat gaatccaatt cctaacgtta ttcttgcccc gatgcaaggg
     1621 gttttagacc cttttgtccg caacctttta acttcagtga atgactacga tctctgtatt
     1681 tccgaatttg tgcgagtggt cgatcaacgt ttaccgaaga aaacctttta tcgcctagcc
     1741 cctgagctat atcaaggcgg attaaccgat tccggcacac cagtgcgggt acagctttta
     1801 gggcaacacc cacagtggct ggcagaaaat gcagctcttg ccattgagct tggttcacac
     1861 ggggtagatc tcaactgtgg ctgcccctct aaaacggtaa atggcagcaa tggtggggct
     1921 tcgttattaa aagatccgga actgatttat cgtgcaacca aagcgatgcg tgaagctgtg
     1981 ccaagcgata aaatcgtttc cgtaaaagtg cgattaggtt gggattccgt tagccaatgt
     2041 tttgaaattg ccgatgcggt agaacagggc ggagcgaatg aaattaccgt tcacgggcga
     2101 accaaagttg atggttatcg agcagaaaga attaattggc aggcgattgg cgaaattcaa
     2161 caacggctgt ctattcctgt tatcgccaac ggcgaaattt gggattttga atcggcgaaa
     2221 aattgccaaa aaataaccgc ttgcaaggca ttaatgatgg ggcgtggggc gttaaacaca
     2281 ccgaatttaa gcaaagtggt gaaatataat gttccgaaaa tggcgtggaa tgaagtgcta
     2341 cagctattgt ttaagtacgt tcagatggaa aatcagtttg ataccggttt ttaccacgtt
     2401 gctcgcatca aacaatggct acgttatttg gataaagaat atcctcaagc ggtagaatta
     2461 tttgatatct tgaaaaccga acacggctat gatggtctaa aagcacatat tgaaaaggca
     2521 gtggaggaat aatgagtaag caaattttta atgaagaaga gctaaaaaca accgagcatt
     2581 ttgaacctaa acaagagttt gatctcgaaa atgcgattat tgaagaagag gaaaaagcag
     2641 ttgaggcgga gctgattatt gaagaaagcc ttaagccttc ccgcttttgg attcgagtac
     2701 tacttgccac acttgccttg tttggcgtgg cggtaattgc tcaaagtatt cagtggttga
     2761 ttgatacgtt ccagcaaaga gagtggatct attttgcgtt ctcgatagtc ttttttattg
     2821 tcagcttgtt gggcgttggg gcgattattt ccgaatggcg aaagttggtg tatttgcgaa
     2881 atcatcagaa aaatcaacaa atgagtaacc aattattgct ggaacagctg ccttccaatg
     2941 atggcgaaca agcggtcaaa ttttgcgaaa atattgcaaa tcagcttaat cacttgccga
     3001 gcattgctca agccgagcaa ctttggaaaa gcgaactgaa cgaggcttat aatgccaaag
     3061 aggtactata tctgtttagc gaaaatgtgc taaagccgat agataaacag gttaagcaga
     3121 tgatttcccg caatgcggca gaaaatgcgg tgattgtggc ggttagcccg ctggcaatag
     3181 ttgatgtgtt gttaatggcg gcaagaaata tcgctttagt gaataaaatt accaaagcct
     3241 atggaatgga attgggttat atcagccgct taaaactgtt caaaatggtg ctgaaaaata
     3301 tggtgttcgc cggtgcaacc gaaatcgcta ctgatgttgg aatggatttc ttctcgcaaa
     3361 accttacggc gaaattatcg ctccgtgccg cacaaggtat tggtgtgggt ttactcactg
     3421 ctcgccttgg cattaaagca atggaatttt gtcgtcctgt tgcttttcaa gcgaatgaga
     3481 agccaagaat ttcagctatt cgtcaggagt tgcttacttc ggtcaaaaat acggtatttg
     3541 ctaaaacaga aacgaaagaa aaagtgttct ctgattagaa aatgctatgg gcggacaatg
     3601 ttccgccctt aatattatcg tttgtattgg cttgttggca cattatccaa atggtcggta
     3661 ttattaaatt ccagcaccgt tactttgcca ttctctactt ctactttcgt aatggcagta
     3721 ttgaacacaa agctatgttt ttcttcattg cggaaatctt gccacgcaat gccttttaac
     3781 acagccgtga gtaaggttaa ggtcatacca tgtgaaacaa tcagcacttt gccgtcattt
     3841 tggtgtagct cggcgatttg attgaacgcc tgagtcaccc gttgataaag ttgctcaaat
     3901 cgctcgccac cgtttgcttt tgccagatat tctgccggtg tacgtttcat cacccaatat
     3961 tcatcatttt cttgcaaatc gacagatttc attccttccc agctgccaaa atcaaattca
     4021 ttcaagcctt tatgatgaaa atgcggaata ttactgcgtt cattttctgc taaaatgtag
     4081 tttgcggtat cttgagcccg tttctgcata ctggaatagg cggcggtaaa cggaatatgt
     4141 tttaacgcaa tgccggtttt cttcgctccg ataatccctt cttctactaa agcggaatcg
     4201 cccgagcctt gtaatctgcc ttccaaattc cacacggttc tgccgtgtcg tactaaataa
     4261 atggtaacag ccattgtaat ttccttgcta aaatagtcaa aaattggaag gattatagca
     4321 aaatgacccc aacattgcct atttgttatc aacatcgtga ttttattatt atcaataagc
     4381 cggaaggctt gagtgtgcat aaagatgagg ctgaaatcgg cttaacggaa ttagtggctc
     4441 gccaactgaa tgtaaagcag gtttggctgg ttcaccgtct ggataaaatc acctcaggtt
     4501 tactgatttt agcgttgaat aaagaggcgg cagcaaggtt ttaccattta tttgaacagc
     4561 ataaaatcta caaaacctat tgggcattaa gcgataaaaa gccgaagaaa aagcagggta
     4621 gaattgttgg cgatatgcaa aaaagcagaa atggagcgtg gaaattatgc cacagtaagg
     4681 aaaacccggc agtaacgcaa tttatttctc attctgttga agactcgctc aggcatttta
     4741 ttttgcaacc caaaacaggc aaaactcacc aattaagagt ggcaatgaaa agtttgggca
     4801 gcccgatttt aggtgataaa ctctattcag gtagccaagc cgaccgtgtt tatttgcacg
     4861 cttatcagct tgattttgaa tacgaaaatg agaaaatctc agtgcaagcc ttgccgacaa
     4921 gcggtcattt ttggcaaaaa atttgcaaat ccaatcacat caaacacgga gaatagtatg
     4981 aaactttggt actcaaccac cagccctttc gcccgcaaag cccttgcggt gattaaacac
     5041 caccaattag acgataaagt cgaaatgttg cgggtgacaa aagcaatgga tccaaattcg
     5101 ccacataatc aagataaccc gttaggacgt attcctgccc tacaacgcaa ctgcggacgt
     5161 tggcttttcg gtagtttgtt aattagcgaa tatcttgacc aaaaaggcaa taacactcca
     5221 ctgttaccta aagagggtaa acctcgctgg gcagtgttgg cattacataa tttggcggac
     5281 ggcattatgg aaaatacgat gccaacttta ttacaagaac gtatggctcg ccccgagaat
     5341 gaatggtggg tcagccgcca cgagcaatta atggaacgca atatccgttc gttcagacaa
     5401 ttagaagagg cattgcaaga gtttggcaca gagctgaatt taggcacatt aaccgcagta
     5461 gcattaattg actggtgggc attccgctta gataagatag gctacgatct tgcaaaaaat
     5521 tacccaaatt taaccgcttg ggcagaggaa atgaataata aatacgccat tctggcagag
     5581 actaagccga gcatgtagag atttgacata gaaaataaag gcattgagtt ataactcaat
     5641 gcctttattt atgaggaaat tgttgttcaa tttcttgcaa tacagataaa ggatctaaat
     5701 ctaacccttt gcaataagcg ataaattcaa aaatatctaa tctgcgatct cctgtttcta
     5761 cttttccgat aaatgagtaa agaacgccca ttttttctcc taatgtgcgt tgtgttagcc
     5821 ctagttccaa acggcgtttt aagaatagtt ctcttaacca aatatgctcg ctggaatgta
     5881 ttgataatct aagattttt
//
LOCUS       DANLXF010000146         5868 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.146, whole genome shotgun sequence.
ACCESSION   DANLXF010000146 DANLXF010000000
VERSION     DANLXF010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5868
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.146"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            132..362
                     /locus_tag="PXD66_001804"
     CDS             132..362
                     /locus_tag="PXD66_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320082.1"
                     /translation="MNAQATLNNHKDYILCGRKEKRTSDFINVFEVFENEATQEFVIE
                     RAMFRNGKLIDWNQSDKMNAEQAQQLWQAYIH"
     gene            744..980
                     /locus_tag="PXD66_001805"
     CDS             744..980
                     /locus_tag="PXD66_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320083.1"
                     /translation="MSNQEFVKLGSVEPSTIDELQKEDIRQAILKSVSITPYYVGLEI
                     AVCQAISAINKFSDGCITEELLDTKNGDKYRKNN"
     gene            1287..1362
                     /locus_tag="PXD66_001806"
     tRNA            1287..1362
                     /locus_tag="PXD66_001806"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1320..1322,aa:Arg,seq:tct)
     gene            1549..2055
                     /locus_tag="PXD66_001807"
     CDS             1549..2055
                     /locus_tag="PXD66_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320084.1"
                     /translation="MKTKYKIIIERAKQQGLTHIELTQKHCLYLATSVGKLCYGVANN
                     AEQHIKLAAGEIFTLLTIINHQIQFDEDLELHTWKASGTTPPEQRAIRMLHHTSQFAW
                     FAPWLVDSNEKEQNQNTIRINLFNKCSGILIELNGILKEYELTLDECLDEFLKNKHCL
                     NEFSIHKE"
     gene            2059..2418
                     /locus_tag="PXD66_001808"
     CDS             2059..2418
                     /locus_tag="PXD66_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320085.1"
                     /translation="MTTFSIYTSVWHDSSTKGFRHNIKHKRHDCWRADIRIKQKINDV
                     WVQISRIRKRFKCPEQAKSFVEQWKQIDSPAFMQHQINRQALREELSNLEQRKQKQDG
                     ILEVIEYRISQIKELLK"
     gene            2415..2894
                     /locus_tag="PXD66_001809"
     CDS             2415..2894
                     /locus_tag="PXD66_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320086.1"
                     /translation="MKRNKVESYTIRLLKNNHTKRYFGEVILNGIKEISTAEMMSEIN
                     AIKAVNKRIEALNKTKAVNLPNYPEIRGHQPIMYNYNFEQIEEDPEQVKPKPSKPKRK
                     PFIPYGLNGYFVDSNGNIRLHLDRRASAETITLETDFFNVLAEMVKRTQEANNAKHA"
     gene            3028..3240
                     /locus_tag="PXD66_001810"
     CDS             3028..3240
                     /locus_tag="PXD66_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320087.1"
                     /translation="MIGYEDYTDASLQPEEDPSEEAFILAEENIIELLENNGFETIAT
                     ILAGSDGWRNAINAYLAEQNKHRNEF"
     gene            3253..4203
                     /locus_tag="PXD66_001811"
     CDS             3253..4203
                     /locus_tag="PXD66_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320088.1"
                     /translation="MTTANQQFELILKTESRVVSTNLQTFEEQANQYLATLTTTFETD
                     DDFAKAKEEVKELESIEKKIRDAIKQTQTGEIAELVLTAEQIAERFRQERLTRDKLVK
                     SKETEIKKQIADKAIEEITDTRNKLVKVSDISLALEITMPKHGIANRIAEAQKNKRTI
                     DSLTKAVNAEKALIISEMSVEIGRLTERLEQLAAKSAYLFPDAVKLIATQDDLAPIIQ
                     QRLAEEAEREAAIKAKAEADKVQAEAKAITDKMERKQAVENPQNVAKMNIEEPAQTDG
                     PLGDFIITIRLNQTIQTNAVSIARELKAKFGDSVSLNKAK"
     gene            4244..5038
                     /gene="bet"
                     /locus_tag="PXD66_001812"
     CDS             4244..5038
                     /gene="bet"
                     /locus_tag="PXD66_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HDL6320089.1"
                     /translation="MTTALQTLTNKLAERFEMGDGSGLVETLKSSAFAGATVSDAQMI
                     ALLVIANQYQLNPWTKEIYAFPGGNGGLTPIVGVDGWVRIINREPQYDGMEFHFTDDY
                     SACTCTIYRKDRSKPIVVTEFMGECKKSSPAWNSHPKRMLRHKAMIQCARLAFGFTGI
                     YDQDEAERIAENEKPPKNITPQNNVVETTAVELISEEQLSQIRQLMQVTGTEEAKILA
                     YIGVQALNQIPKSQAEAVIKKLNLTLDKQNAEKADNGESVGEEIPL"
     gene            5035..5670
                     /locus_tag="PXD66_001813"
     CDS             5035..5670
                     /locus_tag="PXD66_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HDL6320090.1"
                     /translation="MTGDLIILDCEQGSEEWLNARLGIPTATGIENIVTPSGKKSSAW
                     NSYLAELVAESIEGAKDGFKSSDMARGNELEPLARMAYEFETDTEVVQVGGVYLNAEK
                     QLMVSPDGLIPNLKKGLEIKCPKMKTHIKYLLEGGVPNEYLIQVQSALWVTGYETWDF
                     VSYCPEYQKQPLYIYTAERDPVLMKAFDQYIPQFIKSLYALKGVTAPKGAF"
     gene            5683..5859
                     /locus_tag="PXD66_001814"
     CDS             5683..5859
                     /locus_tag="PXD66_001814"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320091.1"
                     /translation="MTTQKKYELLQNDTINHNGRTLYRIKAICHFRRTAEYSARATHA
                     TAAECYGGIACTST"
BASE COUNT         2059 a         1215 c         1222 g         1372 t
ORIGIN      
        1 aatcaattat ttaatcacga attgaaattt gtttggctaa aataattaca aattgaattg
       61 acttaatgat ttcaatttgt gataataaac ccatcaaaac aaacaacgcc acagacaaag
      121 aggaaaccaa aatgaacgca caagcaacgc taaataacca caaagactac attttatgcg
      181 gacgcaaaga aaaacgcacc agtgatttca tcaatgtgtt tgaggtcttt gaaaatgaag
      241 ccacgcaaga gtttgtgatt gaaagagcaa tgttcagaaa tggcaagtta atcgactgga
      301 accaaagcga caaaatgaac gccgagcaag ctcaacaact ttggcaagcc tacattcact
      361 aagaattttt atcaaagccc ttcacggagg gcttgaataa aggttctaaa cctcgccgaa
      421 aggcattgtt ctttaaaaat taaaccaaaa acacatcacg acaagcggat tacttagcaa
      481 tgtaatgaca ttggagagac agaaccaatc tttgcataaa gcggtaaaac gtatgcggta
      541 aatgaactta taggcaagtt gtgaaactct ctaagctact cggactgaaa gaatagtacg
      601 gtagttgtgg cgaatttact ggtgatgtgc gaactagcgg aaaggttaag cactggcagt
      661 gaatcgggaa gcacaaacgc tggttcaatt ccaaagcata tttgagaaag tgtgctttgg
      721 aatattaata acaggagaac aaaatgagta atcaggaatt tgttaaatta ggcagcgttg
      781 aaccttcaac gattgatgaa ttacagaaag aagatattag gcaggcaata ttaaaatccg
      841 tctctataac gccatattat gtggggctgg aaatagcggt ttgccaagca atatcagcca
      901 tcaataaatt tagtgatggc tgtattacag aagagctttt agatacgaaa aatggtgata
      961 aataccgaaa aaataactaa tcaataacac caaagtgatt tcattttttc ggcattagcc
     1021 ctagatgttg cccaagtatt atattcctcg aagttgtggc aaacaagagg acttggcccg
     1081 gtggctcaca agtataaaaa agagccacca tctctaacag ctctcaacag agagtgagac
     1141 aaataacccc gttttagttt taatgttttc taaagtcggc gttaggcagc gaaacgaatg
     1201 tactagagag ttgttagaga tggttagtcc atcacattcc ttagattgaa gtaagccctg
     1261 ccgttggggt taagcaacgg tactttgacc gcatagttca gtggatagag cagctgcctt
     1321 ctaagcagtg ggtcgagagt tcgaatctct ctgcggtcgc catccaaaac cgctttacgc
     1381 acatcatcga tgacgagcct acgaagtgga aacctaccgt gtagagcggt tttgaatggc
     1441 taagttgtgt aactgctaca acttaaaaag agcggttgat aataaacata atgactccgt
     1501 ttccccgcct cgtgcggggc tttttttatc cacataaaag gattcaaaat gaaaactaaa
     1561 tacaaaatta ttattgagcg agcaaaacaa cagggtttga cccatattga gcttactcaa
     1621 aaacattgtc tgtatctcgc tacatcagta gggaaactgt gctacggggt agctaataat
     1681 gcagaacaac atattaaact tgctgctgga gagattttta cactactaac gatcattaac
     1741 catcaaatcc agttcgatga agacttagag cttcacacgt ggaaagctag tggaacaacc
     1801 ccacccgagc aaagagcgat acgtatgtta catcatacct cgcaatttgc ttggtttgct
     1861 ccttggttag tggacagcaa tgaaaaagaa caaaaccaaa atacaatcag gattaattta
     1921 tttaacaaat gctctgggat tctgattgaa ctaaatggca ttcttaaaga atacgagcta
     1981 actcttgatg agtgcttaga tgaattcttg aaaaataagc attgtttgaa tgaatttagt
     2041 attcacaagg agtaaaaaat gacgacgttt agtatttata ccagtgtatg gcacgacagc
     2101 tccacgaaag gctttcgcca caacattaag cataaacgcc acgattgctg gagagcggat
     2161 atccgcataa agcaaaaaat caatgatgtg tgggtgcaga tttcaaggat acgcaaacgc
     2221 ttcaagtgcc ctgaacaggc aaaatccttt gtagaacaat ggaaacaaat tgattcacct
     2281 gcatttatgc aacaccaaat caaccgccaa gcattaagag aagaattatc aaaccttgag
     2341 caacgcaaac agaagcaaga cggaatactt gaggtgattg agtatcgtat tagccaaatc
     2401 aaggagttgc tgaaatgaaa cgcaataaag tagaaagcta tactatccgc ttattaaaaa
     2461 ataatcatac aaaacgctac ttcggagaag ttatattaaa cggcataaaa gaaatcagca
     2521 cggcagagat gatgtctgaa attaacgcta ttaaggcagt aaataagcgc attgaagctc
     2581 tgaataaaac caaagccgtt aatttgccta attatcctga aatccggggt catcagccta
     2641 ttatgtacaa ttacaatttt gaacaaattg aggaagatcc tgaacaggtt aagcctaaac
     2701 catcaaaacc aaaacgcaag ccattcatac cttatgggct aaacggctac tttgtggata
     2761 gcaacggtaa tattcgctta catctagaca gacgagcaag tgccgaaaca attacccttg
     2821 aaactgattt cttcaacgtt cttgccgaaa tggtaaaacg tacacaagag gcaaacaatg
     2881 ctaaacatgc ttagacaatg gattaaatac ggcttatacg ccctgctttt tatggttgcc
     2941 atcaccctcc taatgcaatg aagagcttat ccctgaaagc acctgcaagc ctgaaacttg
     3001 cgaatttaaa gtaacggaga aagtgtgatg ataggatacg aagactacac agatgcttca
     3061 cttcaaccag aagaagaccc tagcgaagag gctttcatct tggctgaaga aaacatcatc
     3121 gagctattag aaaacaacgg ctttgaaacc attgccacca tcctagccgg ttctgatggc
     3181 tggcgtaacg caatcaacgc ctacctagcc gaacagaaca aacacagaaa tgaattttag
     3241 aggaataaga aaatgacaac tgcaaatcaa caatttgaac ttattttaaa aaccgaaagc
     3301 cgagtggttt ctaccaactt gcaaactttt gaagagcaag caaatcaata ccttgccacg
     3361 ctcaccacca cctttgaaac cgatgatgat tttgcaaaag caaaagaaga agtgaaggag
     3421 cttgaaagca ttgagaaaaa aattcgtgac gcaattaaac aaacgcaaac tggcgagatt
     3481 gcggaattgg tattaacagc agaacaaatc gctgaacgtt tccgccaaga gcgattaacc
     3541 cgagacaagc tcgttaagag caaagaaacg gaaatcaaaa aacagattgc cgataaagcc
     3601 attgaagaaa tcactgatac cagaaacaag ctggttaaag taagtgatat ttcccttgcc
     3661 ttagaaatca caatgccaaa acacggcatt gctaatcgta ttgccgaggc acagaaaaat
     3721 aaacgcacga ttgacagctt aaccaaagcc gtgaacgccg aaaaagcgct gattatcagc
     3781 gaaatgtcgg tagaaatcgg gcgcttgact gagcgattag aacagcttgc tgcaaaatca
     3841 gcttatcttt ttcctgatgc agtgaaattg atcgcaaccc aagacgacct cgccccaatt
     3901 attcaacaac gccttgccga agaagccgag cgtgaggcgg cgattaaagc caaagcagaa
     3961 gccgacaaag tacaagccga agccaaagcg attactgata aaatggagcg caaacaagcg
     4021 gtcgaaaatc cgcaaaatgt ggcaaaaatg aacatcgaag aaccggcaca aaccgatggg
     4081 ccattaggcg attttatcat tactattcgc ttaaaccaaa caatccaaac caacgcagtc
     4141 agtattgctc gtgagctgaa agcgaaattt ggggatagcg tttcgctcaa taaggctaaa
     4201 tgatttgata ccgcacaaat tcaacctaaa taaggatcat attatgacaa ctgcattaca
     4261 aacactaacc aacaaacttg ccgagcgttt tgaaatggga gatggctcag gattagttga
     4321 gacacttaaa tcgtctgctt tcgcaggggc aaccgtaagc gatgctcaaa tgattgcact
     4381 attggttatt gctaaccaat accaattaaa cccttggaca aaagaaattt atgcattccc
     4441 cggtgggaac ggtggattaa cgcctattgt cggcgttgat ggttgggttc gcattattaa
     4501 ccgtgagcct caatatgatg gaatggaatt tcattttacc gatgactatt ccgcctgtac
     4561 ttgtactatc taccgtaaag atagaagtaa accgattgtg gtgacggaat ttatggggga
     4621 atgtaaaaag agctctccag cttggaactc tcacccaaaa agaatgttac gccataaagc
     4681 aatgatccaa tgtgcaagat tagcctttgg ctttacgggt atttacgacc aagacgaagc
     4741 agagcgtatt gcggaaaatg aaaaaccacc taaaaacatc acaccacaaa ataacgtggt
     4801 agaaaccact gccgttgaac tcatttcgga agaacaactg tcgcaaatcc ggcagttaat
     4861 gcaagtaacc ggtacggagg aggcgaaaat tctcgcctat attggcgttc aagcactgaa
     4921 ccaaatccct aaatcgcagg cagaggcagt gattaaaaag ctcaatctta cgctagacaa
     4981 gcaaaacgct gaaaaggcgg ataatggcga aagtgtcggt gaggaaatcc cactatgaca
     5041 ggcgatttaa tcattttaga ttgcgaacaa ggatcagaag aatggctcaa tgccagattg
     5101 ggcattccca ccgccacagg cattgaaaat atcgttacgc caagcggtaa aaaatcgtcc
     5161 gcttggaata gctatcttgc cgaactggtt gccgaaagca ttgagggagc aaaagacggt
     5221 tttaagtcgt ctgatatggc acgaggcaac gagcttgaac cgcttgcacg aatggcttac
     5281 gagtttgaaa ccgacaccga agtcgtgcaa gtcggcggtg tgtaccttaa cgccgaaaaa
     5341 cagttaatgg taagtcctga cggcttaatt cccaatctaa aaaaagggtt ggaaatcaaa
     5401 tgcccgaaaa tgaaaaccca catcaaatac ctgctagaag gcggtgtgcc gaatgaatat
     5461 cttattcaag tgcaatctgc cctttgggta acaggctacg aaacgtggga ctttgtcagc
     5521 tactgccccg aataccaaaa acaaccgctt tacatctaca cagccgaacg tgatccggta
     5581 ttgatgaaag cctttgatca atatatcccg caatttatca aatcccttta tgcattgaag
     5641 ggggtaaccg ccccgaaagg ggctttttaa taggtagcca atatgacaac ccaaaagaaa
     5701 tacgaactac tccaaaacga cacaatcaac cacaacggca gaacactcta ccgcatcaaa
     5761 gccatatgcc actttaggcg gacggctgaa tattccgcaa gagccactca cgccacagcc
     5821 gctgaatgct atggtggaat tgcctgtaca tcaacataac cacaaaca
//
LOCUS       DANLXF010000147         5835 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.147, whole genome shotgun sequence.
ACCESSION   DANLXF010000147 DANLXF010000000
VERSION     DANLXF010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5835
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.147"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(29..105)
                     /locus_tag="PXD66_001815"
     tRNA            complement(29..105)
                     /locus_tag="PXD66_001815"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(69..71),aa:Pro,seq:tgg)
     gene            275..1135
                     /gene="folD"
                     /locus_tag="PXD66_001816"
     CDS             275..1135
                     /gene="folD"
                     /locus_tag="PXD66_001816"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HDL6320092.1"
                     /translation="MTAQIISGSEVSSQIKKKVSEKIAHYLEQGKRNPGLAVILVGND
                     PASQVYVGSKRKSCAEIGIASKSYDLPEETTEAELLALIEKLNQDDSVDGILVQLPLP
                     KQIDSTKVIEAILPHKDVDGFHPYNVGRLSQRIPTLRPCTPYGVMKLLETTGVDLYGQ
                     HAVIVGASNIVGRPMALELLLGGCTVTVTHRFTKDLEHHVRQADILVVAVGKPEFIPG
                     EWVKDGAIVIDVGINRGADGKLKGDVEYSVASQKAAFITPVPGGVGPMTVAMLMQNTL
                     QTYENHLQAV"
     gene            1249..2700
                     /gene="thiI"
                     /locus_tag="PXD66_001817"
     CDS             1249..2700
                     /gene="thiI"
                     /locus_tag="PXD66_001817"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDL6320093.1"
                     /translation="MKFIIKLFPEIMIKSDSVRKRFIKILTSNIRNVLLKETENVSVV
                     RHWDFIEVRAKVDEETALVLDLLKRTPGIHHILEVEETPFTDMHDIFEQTYAAVKAEL
                     EGKTFSVRVRRKGKHDFRSLDVERYVGGGLNQRIETAKVKLTNPDVTVRIDIEHDKML
                     LIKARHEGLGGYPIGTQEDVLSLISGGFDSGVSSYMLIRRGSRVHYCFFNLGGAAHEI
                     GVKQMAYHIWSRYSTSHKVRFIAINFENVVGEILEKVDNGQMGVVLKRMMVRAASRIA
                     ERFDIQAIVTGEALGQVSSQTLTNLRLIDKASDTLVLRPLITHDKEQIIAMAKAIGTD
                     DIAKSMPEFCGVISKNPTVKAIETKILEEEGNFNFEVLEQAVQTASYLDIREIAQQTE
                     KEVVSVETTTELSKNDVVLDIRSPEEVDEKPFRLDGVEVKELPFYKLSSQFSTLDQSK
                     TYLLYCQRGVMSKLQALYLKENGYSNVKVFRVK"
     gene            complement(2745..3452)
                     /locus_tag="PXD66_001818"
     CDS             complement(2745..3452)
                     /locus_tag="PXD66_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiC-like family protein"
                     /protein_id="HDL6320094.1"
                     /translation="MFKEKPVISNQHGALAMAMLPFLYAVIESGFSTLHIFFGLSWLF
                     LYFFSYPFLSLFSKKPTERNKKWAKIYFGLSILFALPLIYAQPSILQFLPIILPLGLV
                     QVHYAKQKDERNLWNDIAGILTFGIMGMAAYYLVTKQYQFEILLHPTLFFIATTFYIK
                     SMVRERRNPIYMEISIAIHLVLAMLYILCSLQAVFFAYLIATARAIIVPSFGWNVKQV
                     GMLEFPVILLFLASLIW"
     gene            complement(3520..4494)
                     /gene="pfkA"
                     /locus_tag="PXD66_001819"
     CDS             complement(3520..4494)
                     /gene="pfkA"
                     /locus_tag="PXD66_001819"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDL6320095.1"
                     /translation="MSKKIKKIAVLTSGGDAPGMNAAIRGVVRTALNEGLEVCGIQDG
                     YYGLYHDKVIPLDRRSVSETINRGGTFLGSARFPDFKRPEVRKHCVETLKKYSIDALV
                     VIGGDGSYMGAKLLTEEFGYPCIGIPGTIDNDIVGTDYTIGYQTALETALDAIDRLRD
                     TSSSHQRISIVEIMGRHCGDLTLSAALAGGCEYVIVPEKGLDKDALMRSIEEGFHKGK
                     RHSIIAITELMTDVHALAKEIEARFGNETRATVLGHIQRGGSPCAFDRILASRMGVYA
                     VELLLQGFGGHCVGIQNEQLVHHDIIDAINNMRRPFKEELFEASRKLF"
     gene            complement(4601..5797)
                     /locus_tag="PXD66_001820"
     CDS             complement(4601..5797)
                     /locus_tag="PXD66_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL6320096.1"
                     /translation="MLNTQFRTHFPFFEKSDGWTYLDSAATTLKPDVLIEATSEFYAS
                     AGSVHRSQYDLPQTEQYELARDLVAKRFNVETREAVIWTSGTTQSINTVAYGLEHQIA
                     AGDEIVISVAEHHANFIPWQQLAQRTRAKLVVLPLNDKYEIELETLKNALSEKTKIVA
                     FNLVSNVTGVRQPAEQIIPLIRQSCSAKIVLDIAQAVCSEQVDVQKLDADFYAFSAHK
                     MYGPNGVGVLTGKLQSLEQIQPLVFGGKMLKNITESELTVAELPYRLEAGTPNIAGII
                     GFGQVLAWLEKWDFVALNHQLYDLAEYTCKRLNSYQNLQIIGQQNTPTISFVVKNQHP
                     ADIAAFLTQSKIAIRQGEHCAKPYLRHLEEVGTVRLSLAHYNNQQDVEHFFNALDFTL
                     EILQES"
BASE COUNT         1738 a         1133 c         1292 g         1672 t
ORIGIN      
        1 gcgcagatga agataatata aaaagaagtg gtcggcgaga taggatttga acctacgacc
       61 cactggtccc aaaccagttg cgctaccaag ctgcgctact cgccgacata agatgttgtt
      121 gattatagat ttctttgcac aacagtcaat ctttttttgt gaatgtcggc ttaaatgatt
      181 gaagtttatc cgttattcaa aattagctta tcaaaaaggc tattttttga gagaatgacg
      241 aatgttttgc caagtgatat ttcgggaggt agtaatgact gcacaaatta tttcggggag
      301 tgaggtttct tcacaaatta aaaaaaaggt gagtgagaaa atcgctcatt atcttgaaca
      361 gggtaaacgt aaccctggtc ttgcggtcat tttagtgggg aatgacccag cctctcaagt
      421 gtatgtgggg agtaaacgca aaagctgtgc cgaaattggg attgcgtcga aatcttatga
      481 tttacccgaa gaaaccacag aggctgaatt attagcactg attgaaaagt tgaatcaaga
      541 tgattcggtc gatgggattt tagtccaatt accgttacct aaacagatcg attcgactaa
      601 agtgattgaa gccatcttac cacataaaga tgttgatgga ttccaccctt ataatgttgg
      661 gcgtttatct cagcgtattc ccactttacg cccttgcacg ccatacggtg tgatgaagtt
      721 gcttgaaaca actggggtag atttatatgg tcaacacgca gtgattgtag gtgcgtctaa
      781 tattgttggt cgcccgatgg cgttagaatt gttgcttggt ggttgtacag tcacagttac
      841 tcatcgcttt accaaagatt tagagcatca cgttcgccaa gcagatattt tagttgtggc
      901 agtcggcaag cctgaattta ttcccgggga gtgggttaaa gacggtgcta tcgtgattga
      961 tgtagggatt aaccgtggag cagacggcaa actaaaaggt gatgtagagt attcagttgc
     1021 ctcacaaaaa gcggcattta tcacccctgt ccccggtggg gtaggcccga tgacggtggc
     1081 aatgttaatg caaaatacct tgcagacgta tgaaaaccat ttacaagcgg tctaatttta
     1141 cggatatttt gcaaatcctc ggcattggtc gaggatttct ttttctgggc tttttcagca
     1201 gaaaaggcta aaatacttgc catttttatt acacgatcct tattaaatat gaaatttatc
     1261 atcaaacttt tccctgaaat tatgattaaa agcgactctg ttcggaaacg ctttatcaaa
     1321 attttaacct caaatattcg caacgtatta ttaaaagaga ctgaaaatgt gtctgtggtt
     1381 cgccattggg actttattga agttcgggcg aaagtggacg aagaaacggc attagtgctg
     1441 gatttgctta aacgcacgcc gggtattcac catattttag aagtggagga aacccctttt
     1501 acggatatgc acgatatttt tgagcagact tatgccgcag tgaaagctga actagaaggc
     1561 aaaacgttta gcgtgcgggt tcgccgtaaa ggaaagcacg atttccgttc tcttgatgta
     1621 gagcgttatg tcggtggtgg gctgaatcag cggattgaaa cggcgaaagt gaaattaacc
     1681 aacccagatg ttacggtcag aattgatatt gaacatgata aaatgttgct gattaaagct
     1741 cgccacgaag ggttaggcgg gtatccaatc ggcacacaag aagatgtgtt gtcattgatt
     1801 tcgggtggtt ttgattcggg tgtatcgagc tatatgctga ttcgcagagg ttctcgtgtg
     1861 cattactgtt ttttcaattt aggtggtgcc gcacacgaaa ttggcgtgaa acagatggca
     1921 tatcatattt ggagccgtta tagtacctcg cacaaagtcc gttttatcgc cattaatttt
     1981 gaaaatgtag tgggtgagat tttagaaaaa gttgataacg gacaaatggg cgtggtatta
     2041 aaacgtatga tggtgcgagc ggcaagccgt attgccgagc gttttgatat tcaagcgatt
     2101 gtgaccggtg aggcgttggg gcaggtctct agccaaacac tgactaattt acgtttaatt
     2161 gataaggcat cagatacgct ggtattacgt ccgctcatca ctcacgataa agagcagatt
     2221 attgcaatgg caaaagcgat aggcactgac gatattgcta aatcaatgcc ggagttctgt
     2281 ggagtaattt ctaaaaatcc gacggttaag gcaatcgaaa ctaaaatttt ggaagaagaa
     2341 ggcaacttta attttgaggt actagaacaa gcggttcaaa cggcgagtta tttagatatt
     2401 cgtgagatcg ctcagcaaac cgaaaaagag gttgtttcgg ttgaaaccac aacagaattg
     2461 agtaaaaacg atgttgtgct agatattcgc agcccggaag aggttgatga aaaaccattc
     2521 cgtttagacg gggttgaagt aaaagaactc ccgttttata aactttccag ccagtttagt
     2581 acgcttgacc aaagcaaaac ctacttgctc tattgccaac gtggggtaat gagtaaactg
     2641 caggctcttt atttgaaaga gaatggctac agcaatgtaa aagtgtttcg agtgaaataa
     2701 tgcctctttg attgcacctt aatgattgat taaggtgcaa ttttttacca aataaggctt
     2761 gctaaaaaga gtaagatcac aggaaattca agcattccaa cttgtttaac attccagcca
     2821 aagcttggca caataatggc tcgggcagtt gcaattaaat aagcaaaaaa gaccgcttgt
     2881 agagagcaga gtatgtaaag cattgctaaa accaaatgta ttgcaatact gatttccata
     2941 taaattgggt ttcttcgctc tcgcaccata ctctttatat agaaagtcgt agcaataaaa
     3001 aatagggtag ggtggagcag aatctcaaac tgatattgtt tcgttaccag ataataagct
     3061 gccattccca ttatgccaaa ggttagaatg ccggcaatat cattccataa attgcgttca
     3121 tctttttgtt tggcgtagtg aacttgcact aacccaagcg gtagaattat cggtaaaaat
     3181 tgcaaaatcg aaggctgagc gtagatcaaa ggtaatgcaa atagaatgct taagccaaag
     3241 tagattttcg cccatttttt attgcgttca gttggctttt tactgaaaag tgagagaaaa
     3301 gggtaggaaa agaagtagag aaacagccac gacagcccaa agaaaatatg caaagtgcta
     3361 aaaccacttt caataacggc gtagagaaac ggcaacattg ccattgctaa tgcaccgtgt
     3421 tggttggata taacgggttt ttctttgaac attcaatttc cctcttaatg tggcaaataa
     3481 aaaggcgact aaacagccgc ctttaaattg taagaaaagt tagaataatt ttctagaggc
     3541 ttcaaataat tcttctttga acggacgacg catattattg atcgcatcaa taatatcgtg
     3601 gtgaaccaac tgctcgtttt gaataccgac acagtgaccg ccaaagcctt gcagtaataa
     3661 ttccaccgca tagacgccca tacgggaggc caaaatacgg tcgaaggcac aaggcgaacc
     3721 accacgttga atatgaccta atacggttgc acgggtttca ttgccgaaac gtgcttcaat
     3781 ttctttcgct aatgcgtgaa catcggtcat caattctgta attgcaataa ttgaatggcg
     3841 tttacctttg tggaaacctt cttcaatgct tcgcattaat gcatctttat ctaagccttt
     3901 ttccggtacg atcacatatt cgcaaccacc ggcgagggca gcacttaggg ttaaatcacc
     3961 acagtggcga cccataattt ccacaatcga aatacgttgg tgtgaacttg aagtatcacg
     4021 caggcggtca atcgcatcta atgcggtttc aagggcagtt tgatagccga tggtgtaatc
     4081 cgttcccaca atatcgttat caatcgtacc cggaataccg atacaagggt aaccaaattc
     4141 ttcggttaat aatttagccc ccatataaga accgtcgcca ccaataacca ctaatgcatc
     4201 aatgctgtat ttttttaagg tttcgacaca atgtttgcga acttctggtc ttttaaaatc
     4261 agggaagcgg gcagaaccta agaatgtacc gcctcggttg atggtttcag acacggaacg
     4321 gcggtcaagt gggatcactt tatcgtggta aagcccatag tagccgtctt gaataccaca
     4381 aacctctaaa ccttcattaa gtgcggtacg aaccacacca cggattgccg cgttcattcc
     4441 tggtgcatca ccaccactgg ttaatactgc aatttttttg atttttttac tcatttaaaa
     4501 acaccttgtt gataaattaa agttaaattg gaaactggtg caaggttaca atattcagcc
     4561 ttttcgttct actaaaaaat acgtattttt ttgatttagc ttagctttct tgcaaaattt
     4621 caagggtaaa atctagagcg ttgaaaaaat gctcaacatc ttgttgatta ttgtaatggg
     4681 caagtgaaag tcgaactgtt ccgacctctt ctaaatggcg taaataaggc ttggcacaat
     4741 gctccccttg gcggatcgca attttacttt gggttaagaa agcggcaata tcggcagggt
     4801 gctgattttt caccacaaag ctaatagtcg gtgtattttg ttgcccgata atttgcaaat
     4861 tttgataaga atttaaccgc ttgcaggtgt attcagccaa gtcataaagc tgatgattaa
     4921 gagcgacaaa atcccatttt tcgagccaag ctagcacttg cccaaagccg ataatgccgg
     4981 cgatattcgg cgtgccagct tccaagcgat aaggtaactc tgccacagtt aattcacttt
     5041 ccgtaatgtt tttcagcatt ttgccgccaa atacaagcgg ttgaatttgc tccaaacttt
     5101 gcaatttgcc ggtaagcacg cccacaccgt ttggaccata cattttatgg gcggaaaaag
     5161 cgtagaaatc tgcatctaat ttctgaacgt ccacttgctc tgagcaaact gcttgagcaa
     5221 tatctaacac aattttagca gagcaagatt ggcggataag cgggattatt tgttccgctg
     5281 gctggcgaac tcctgttacg ttagaaacta aattaaaggc aacaattttg gttttttccg
     5341 ataacgcatt tttaagggtt tcaagctcaa tttcatactt atcattaagt ggcaacacca
     5401 cgagtttcgc ccttgtacgc tgtgccaact gctgccaagg aataaaatta gcgtgatgtt
     5461 ccgccactga aattacaatt tcatcgcctg ctgcaatttg atgctccaag ccataagcca
     5521 cggtgttaat actttgggtt gtaccgcttg tccaaatcac cgcttcacga gtttccacat
     5581 tgaaacgttt tgcgactaaa tctcgagcta attcatattg ttcggtttgt ggcaaatcgt
     5641 actgactgcg atgcaccgac cccgcagaag cgtaaaactc acttgttgct tcaatgagta
     5701 catcaggttt cagcgttgta gcggcagaat ctaaatacgt ccatccgtca gatttttcaa
     5761 aaaaagggaa atgggtgcga aattgggtgt taagcataag ttaattccta agagttgtgg
     5821 aaaattgagt tacca
//
LOCUS       DANLXF010000148         5820 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.148, whole genome shotgun sequence.
ACCESSION   DANLXF010000148 DANLXF010000000
VERSION     DANLXF010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5820
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.148"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(137..1324)
                     /locus_tag="PXD66_001821"
     CDS             complement(137..1324)
                     /locus_tag="PXD66_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HDL6320097.1"
                     /translation="MSNNNRSALLGAAFLMATSAIGPGFLTQTATFTQTLLASFGFVI
                     LLSILLDIGAQLNIWRIVAVSEKKAQDIANAVLPGAGYFLALLIVMGGLAFNIGNVGG
                     AGLGLNILTGLSPEMGAVVSGAIAVGVFLFHEAGKVMDRFAQIMGFVMIALTIFVAVK
                     ANLPIDDAVVHTFVPEKLDVIAIVTLVGGTVGGYITFAGAHRLLDAGIKGKENLKEVS
                     KSSVSAILIASIMRVVLFLAVLGVVSMGVQLDPKNPAATPFAHVAGDVGLMIFGVVIW
                     AASITSVIGAAYTSVSFITSFSPFIEKHKNCFIVAFIVVSTAVLATIGRPAQVLVFVG
                     TLNGLILPISLGLILLAAHKTKIVGDYKHPVWMTVSGAIVVILMAVLSAMTLSKYISG
                     LMA"
     gene            complement(1599..2336)
                     /gene="pxpA"
                     /locus_tag="PXD66_001822"
     CDS             complement(1599..2336)
                     /gene="pxpA"
                     /locus_tag="PXD66_001822"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HDL6320098.1"
                     /translation="MNHVDLNVDLAEGCGNDHSLLQLVTSANVCCGLHAGDYNEMRKA
                     INWAKENSVRVGAHPSFPDRENFGRTNMQLPDDELKACLLYQLGAAKALCDAENMPMS
                     YVKPHGALYNMAAKDPHLATLIANTVKQFDPNLKLMGLSGSLMLSIAEQHGLETISEV
                     FADRHYLADGSLVPRTRDDAMVESDDEAISQVLQMVLDGTVPTVDGGNVAIKADSICL
                     HGDGEHAVVFAERIKKELQAKGILVKA"
     gene            complement(2323..3236)
                     /locus_tag="PXD66_001823"
                     /pseudo
     CDS             complement(2323..3236)
                     /locus_tag="PXD66_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250478.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
     gene            complement(3233..3880)
                     /gene="pxpB"
                     /locus_tag="PXD66_001824"
     CDS             complement(3233..3880)
                     /gene="pxpB"
                     /locus_tag="PXD66_001824"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDL6320099.1"
                     /translation="MLTIHHTSEHSLLCLAEPPAELAKQQQIWRVAEQARALDGVLEV
                     VVGMNNFTLFHAIGTDSTRLIQAVQQLWENDQAQAAQSPRLIEIPVHYGGEFGEDLFE
                     VAQFHNTTPEEIIRRHTEPTYTVFMIGFQPGFPYLNGLPQNLHTPRRNVPRTKVPAGS
                     VGIGGRQTGIYPFTSPGGWQLLGRTELPLFDIQHNPPTLLTAGDNVRFVAESITL"
     gene            complement(3974..4798)
                     /locus_tag="PXD66_001825"
     CDS             complement(3974..4798)
                     /locus_tag="PXD66_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HDL6320100.1"
                     /translation="MKKLLLFLLFIGNPIFAKSPELMLVEQYQDQDISGWVMSEKLDG
                     VRGFWDGQQLISRQGYPLNPPDYFIQDFPPFAIDGELFSERGKFEEISSIVRSAQPKG
                     WHKLKLQVFDVPNAQGNLIERLATLENYLKQHPTLHIAIIEQIPIKDKAHLQQFYQSV
                     LEQGGEGVIVRNPNAGYIQGRSAQILKIKPVLDEECTVVAHHKGKGKYTDKLGAITCE
                     NQRGRFRIGSGFKDKERENPPPIGSIITYKYRGVTESGKPRFATFWRIRNDQISEN"
     gene            complement(4932..5756)
                     /locus_tag="PXD66_001826"
     CDS             complement(4932..5756)
                     /locus_tag="PXD66_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320101.1"
                     /translation="MEHFIGLGVAGNFAGHLEQAGEEADFAKVKTVEAVQPKAIFPFY
                     VPAENLGDYQFLSTYPLSNTAINFPSDADNLQIEPEVALICEIGYQDQQVVSLTPTHF
                     AAYNDCSIRRPNANKICEKKNWGASSKGLSAIHLPLDNFNKGGTLDNYNIACFHKRNG
                     ELNTYGIDSPAVEYSYFHQKLLDWIVERMNNQPDEGPMNNISEMLKQANYPTKAIISI
                     GATRYTPFGESNLLQVGDVSMVVVYNVKKYSHSQIEEMAKLETFSEDISALIQTVR"
BASE COUNT         1526 a         1385 c         1329 g         1580 t
ORIGIN      
        1 ttatttcaaa ttatttagcc tcaaaatgag tggtagatca caaactttaa acgcaagcgg
       61 tcagatttta caaatttttt acaagagtag ttaaaatttg caaaacctga ccttaggtta
      121 gcttgaagct aagtcgttat gccattagcc ccgaaatata ttttgaaagg gtcattgcac
      181 ttagcaccgc cattagaatc accacaatcg cccctgaaac ggtcatccac acagggtgtt
      241 tgtagtcgcc cacaatcttg gttttgtgag ctgccagcag aattaacccg agggaaatcg
      301 gtagaattaa gccgttaagc gtccctacaa acaccaagac ttgtgccggt ctgccgatgg
      361 ttgccagcac ggcggttgaa accacaataa aggcgacaat gaaacagttt ttgtgttttt
      421 cgataaatgg cgagaatgaa gtaatgaagg aaaccgaggt ataagccgcc ccaatcacag
      481 aggtaatgga ggctgcccaa atcaccacgc cgaagatcat caaaccgaca tcgccggcaa
      541 cgtgggcaaa tggggtagcc gcagggtttt tcggatcaag ttgcacgccc attgacacca
      601 cgcccagcac cgctaaaaac agtaccacac gcataattga ggcgattaaa atggctgaca
      661 ccgagctttt gctcacctct tttaaattct ctttgccttt aatgcctgca tcaagcaaac
      721 ggtgcgcacc agcaaaggtg atgtatccac caacagttcc gcccactagc gtgacaatcg
      781 caatcacgtc taatttttcc ggcacaaagg tatgcaccac cgcatcgtcg attggcagat
      841 tggcttttac ggcaacgaag atggttaaag caatcatcac aaagcccata atttgtgcaa
      901 atctgtccat cactttacca gcttcatgga acaggaacac gcccacggca atcgcaccgc
      961 tgaccaccgc acccatttca ggggaaagtc cggtgagaat gtttagccct aaacctgcac
     1021 cgcccacgtt gccgatatta aaggctaaac cgcccatcac gataagcaga gccagaaagt
     1081 aacctgcccc cggcaatacg gcattggcga tgtcttgagc tttcttttcc gatactgcta
     1141 caattcgcca aatattcagt tgagcaccga tatcaagcaa gatcgagagt aagatcacaa
     1201 agccgaagct ggcaagtagg gtttgggtga aggtggctgt ttgggttaag aaccccggcc
     1261 cgatggcgga ggtcgccatt aaaaatgctg ccccgagtag ggcgctacgg ttgttgtttg
     1321 acattgtgtt ggttcctttt ttgttgttaa tgagtcatta ttcgtggatt tatcatggta
     1381 atattcccct ctttagcaaa gaggggttag gggagatttg gcagtagaac tcatcgaagt
     1441 gagagagtta ataatgctca atttaacctc ttgaaattac ttattttctc cgtaaatcgc
     1501 ttctgtcaaa tctcccccag cccctctttt ctaaagaggg gagttcgatc tccaagagtt
     1561 ttatttctgg ttttccgtcc attttgacta aaacaggatc aggctttcac caaaatcccc
     1621 tttgcctgta actctttttt aattcgctcg gcaaatacca cggcgtgttc gccgtcaccg
     1681 tgtaagcaaa tgctgtcggc tttaatcgcc acattaccac catcaacggt tggtacagtg
     1741 ccgtctaaca ccatttgcaa gacttggctg atcgcttcat catcgctttc taccattgca
     1801 tcatcacggg tgcgaggcac gagcgagccg tcagctaaat agtggcggtc ggcaaagact
     1861 tcggaaatgg tttccaaacc gtgctgctcg gcaatgctca acatcaggct gccggataag
     1921 cccatcagtt tgaggttggg atcgaactgt ttgaccgtat tggcgataag ggtggcaagg
     1981 tgagggtctt tggcggccat attgtataaa gcgccgtgcg gtttaacgta gctcatcggc
     2041 atattttccg catcacataa ggctttggcg gctcccagtt ggtagagcag acaggcttta
     2101 agttcatcat cgggcagttg catattggta cgcccaaagt tttccctatc tggaaaactc
     2161 gggtgagcgc caactcgcac gctgttttcc ttcgcccagt taatcgcttt acgcatttca
     2221 ttgtaatcac cggcgtgcaa gccacagcag acattcgccg aagtaacgag ctgtaataat
     2281 gaatgatcgt tgccacaacc ttcggcaaga tcgacattta aatcaacgtg attcattcgc
     2341 ttttctccga attgtttcca aataattttg atctttttga taaatttcga ccgcttgttc
     2401 acggcttact tgctcaaatt gcaccgttga gccgaacgga ttttgtgcca accgcccgag
     2461 atcggcctga ataatgcagg caattttcgg ataaccaccg gtggtttggg catctgccat
     2521 cagcacaatc ggctggccgt ccggcggcac ttgcaccgta ccactcgggg ctgcgtagga
     2581 gagcatttca agggaagcgg tcaattccaa cgctttttct gcaaaacggt agcccatacg
     2641 gttgctgttg ttttgcagtt gccacccttg ttgccaaaag cgttcacggc tatctgcggt
     2701 aaaggcttca tattcgctcg aagcggttgc ccgaatgcgt gaggtgaaat caatcggctc
     2761 aatcgacatc aaagaaagtc ggctgtctct gcggcctgtg gcgatgttat cgccggcttt
     2821 taacattctg ccttgaaaac cgccaaattg agctttgaga gcggtacttg ccgaacctaa
     2881 cacctgcggc accagaaaac cgcctgccac gcataaatag gcgtaattgc cgagaacaat
     2941 ccgttttatc gtcagcgttt gacgggcgtt ggcagtgtag cggtaatagg aaaaaaccgg
     3001 cgtgccgtcc agctccgcct ccaccaatgc accggtgagg caaaatggcg tgtcgcaatc
     3061 acgccaaggg taagttcaat cgcaggtgca ttatttgcat tttttaacaa taaattaccc
     3121 gcttgtaagg caaggctgtc catcgcaccg ccgtggctca cgcctaaatg gcgataacca
     3181 taacgcccta aatcttgaat gtgggcaaaa ccctgtatta tttcgatata aatcatagcg
     3241 ttatactctc cgccacaaat ctaacattat cgccggctgt aagtagcgtt ggcggattgt
     3301 gttgaatatc aaacaacggt aactcggttc gtcctaacag ttgccagcca cctggtgagg
     3361 taaagggata aatgcctgtt tgcctgccgc cgatgccgac cgaaccggca gggactttgg
     3421 tgcgaggcac gtttcggcgt ggggtgtgta aattttgcgg taagccgttg aggtaaggaa
     3481 agccgggttg aaagccgatc ataaacacgg tgtaagtcgg ttcggtatga cggcggataa
     3541 tctcttcggg agtggtattg tggaattggg cgacttcaaa cagatcttcg ccaaattcac
     3601 cgccgtaatg gacagggatt tcaatcaggc gtggggattg tgcggcttgg gcttggtcgt
     3661 tttcccagag ttgttgaacc gcttggataa ggcgtgtgct atcggtgcca atggcgtgaa
     3721 atagggtaaa attgttcatt cctaccacca cttccaacac gccatctaag gctcttgcct
     3781 gttctgccac ccgccaaatc tgttgctgtt tggcaagctc tgcaggcggt tcggctaagc
     3841 agagcaggga atgttcgctg gtatgatgaa tcgtcagcat aattgccctc aaattgttac
     3901 aaaaaggtaa caacttttta cgctgatata tccagactgt caattttatt ttgttgaaaa
     3961 tgtgaaatag ctcttaattt tcagaaattt gatcatttct tattcgccaa aacgttgcaa
     4021 atctgggctt gcctgattct gtgacacctc gatatttata ggtaatgatt gagccaatcg
     4081 gtggcggatt ttctcgttct ttatctttaa aaccgctgcc aatccgaaaa cgtccacgtt
     4141 gattttcaca agtgattgcc ccgagtttat cggtatattt ccctttgcct ttatgatggg
     4201 caaccacagt gcattcctca tctaaaaccg gtttgatttt caaaatttgg gcagaacgac
     4261 cttgaatata gcctgcattc gggttgcgaa cgataacccc ttcgccacct tgttccagca
     4321 cagattggta aaattgttga aggtgggctt tatctttaat cggaatttgc tcaataatag
     4381 cgatatggag cgttggatgt tgcttcagat aattctctaa ggtcgccagc cgttcaatca
     4441 aattcccttg tgcatttggc acatcgaaca cctgtaattt cagcttgtgc caacctttgg
     4501 gctgagcgga acgcacaatg cttgaaattt cctcaaattt gccccgttcg ctaaacagtt
     4561 cgccgtcaat cgcaaaagga gggaaatctt gaataaaata gtcgggtgga ttgagcggat
     4621 aaccttggcg gctgatgagc tgttgaccgt cccaaaagcc tcgcacgcca tcgagttttt
     4681 ctgacattac ccagccactg atgtcttgat cttgatattg ctcaacgagc attaactcgg
     4741 gcgatttggc aaaaataggg ttaccaatga agagtaggaa taagagcagt tttttcatag
     4801 tctctccgaa atagtgcctc cgaatatgca aggcactata aaggaaagcg tgcaaacagt
     4861 cagaaatgaa tctcaatttt tcgacgcaga tcgcaaaatt ttccgaaaaa ctgatcgctt
     4921 gagtgagaaa tttaacgtac tgtttgaatt aaagcagaaa tatcctcgct aaaggtttcc
     4981 aacttcgcca tttcttcaat ttggctatga gaatatttct tcacattgta aacgaccacc
     5041 atacttacat cacctacttg caataaatta ctttcgccaa aaggcgtgta gcgtgttgcc
     5101 ccaatgctga taatcgcttt agttgggtag tttgcctgtt ttaacatttc ggaaatgttg
     5161 ttcatcggac cttcatcggg ctgattattc attctctcta caatccaatc gagcaatttt
     5221 tgatggaaat agctgtactc caccgcaggg ctgtcgatgc cgtaggtatt cagctcgcca
     5281 ttacgtttat ggaaacaagc gatgttgtaa ttatccagcg tgccgccttt gttgaagtta
     5341 tctaacggaa gatgaattgc cgaaagccct ttagacgaag ccccccagtt tttcttttca
     5401 cagattttat tcgcattcgg acggcggata gagcagtcat tgtaagccgc aaaatgggtt
     5461 ggcgtgagcg aaaccacttg ctgatcttgg tagccaattt cacaaattaa cgccacttca
     5521 ggctcaattt gcagattatc agcatcactc gggaaattaa tcgcagtatt tgaaagcgga
     5581 taggtggata aaaactgata atcccccaaa ttttccgccg gcacataaaa cgggaaaatc
     5641 gcctttggct gcactgcttc cactgttttc actttcgcaa aatccgcctc ctctccggct
     5701 tgttctaaat gtccagcaaa attgccggcc acgcctaaac cgataaagtg ttccataatc
     5761 tactccttaa ttggtaaaag ttggataaaa ttttaccgct tgtaaccaaa aattgcgaat
//
LOCUS       DANLXF010000149         5765 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.149, whole genome shotgun sequence.
ACCESSION   DANLXF010000149 DANLXF010000000
VERSION     DANLXF010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5765
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.149"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            95..3166
                     /gene="fdnG"
                     /locus_tag="PXD66_001827"
     CDS             95..3166
                     /gene="fdnG"
                     /locus_tag="PXD66_001827"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014550636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:680..682,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HDL6320102.1"
                     /translation="MQISRRKFFKVCAGGMAGTSAAMLGFAPATALAAPREYKLLRAK
                     ETRQTCTYCAVGCGMIMYSLGGEAMNSKGKLIHIEGDSDHPVSRGALCPKGAGALDYV
                     NSDRRVHYPEVREAGSSEWKRISWHEAIERIARHMKNDRDENFVTHNEAGLEVNRWMT
                     AGFLAGSACSNETGIVTQKFIRSLGMIFTDNQASIUHGPTVASLAPSFGRGAMTNHWV
                     DIKNSDLVIVMGGNAAEAHPVGFRWAVEAKKQNGAKLMVVDPRFNRSAAVADIYIPLR
                     PGTDIAFLLGAVNYLLKNDKIQHEYVKHYTNATFLLNEGFKFEDGLFTGYDEATRTYK
                     DKSTWAYQLDEQGQPKRDMTMQDPRCVINMLKDHVSRYTPEMVERITGTPQKDFLIFC
                     EEIAKTSAPDKAATFLYALGWTQHTVGAQNIRSMAIIQLLCGNIGVSGGGVNALRGHS
                     NVQGITDLGLFPHMLPGYIRLPTEADATLESYLNAIAPKTLVAGQVNYGQNNPKFAVS
                     MLKTFYGDKATKENEFGYHYLPKYPKGGTDHFRFIEQMYQGKVNGYFCQGMNPIASYP
                     NSQKLIKALSNLKYLVIFDPLITDTSEFWKNHGEFNNVNPSEIQTEVFRLPTTCFAEE
                     DGSIANSGRWLQWHWKAADAPHEAKPDVDILSEIRAELIHLYHKEGGKAPLEPLEAMT
                     WNYMNPLEPKPEEIAKENNGYALEDLKDADGNIIVKKGELLSSFTQMRDDGTTSGGCW
                     IYCGQWTPKGNQMANRDNSDPSGLGNTLGWAFSWPVNRRILYNRASADLSGKPWNPKR
                     QLVKWNGKNWNYVDVADYGTAPPNSPVTPFIMQPEGVSGLFVRERMQDGPFPEHYEPF
                     ETPIGTNPLHPNVISSPVARILPSDKEDLGTSDEFPYVGTTYRLTEHFHYWTKNALIN
                     VIAQPEQFVEIGEKLAEEKGIKNGDKVRVWSKRGEIKAVAVVTKRIRSLVSDGKPIHT
                     IGVPIHWGFAATTGAKKGYFANNLTVRTGEPNSNTPESKCMLLNIEKIVGA"
     gene            3169..4083
                     /gene="fdxH"
                     /locus_tag="PXD66_001828"
     CDS             3169..4083
                     /gene="fdxH"
                     /locus_tag="PXD66_001828"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HDL6320103.1"
                     /translation="MSTVQTQNIIKISATSALTPAPQARSHQVEVAKLIDVTTCIGCK
                     ACQVGCSEWNDIRSDVNANCVGVYDNPADLNAKAWTVMRFNEVEENDRLEWLIRKDGC
                     MHCAEPGCLKSCPSPGAIIQYANGIVDFQSDKCIGCGYCIAGCPFNIPRMNPDDNRVY
                     KCTLCVDRVNVGQEPACVKTCPTGAIRFGSKEEMKFYAEQRIADLKSRGYENAGLYDP
                     EGVGGTHVMYVLHHADKPELYSGLPKDPKIDTTVTLWKDVLKPVAAVALGGLALAEVA
                     HYITVGPNVEEDLEEHHHSENAEGGKHE"
     gene            4076..4753
                     /locus_tag="PXD66_001829"
     CDS             4076..4753
                     /locus_tag="PXD66_001829"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HDL6320104.1"
                     /translation="MSKKFEITNDTKIVRHKPLARVSHWFLVISFFMTMFTGVAFFFP
                     DFAWLTEILGTPQIARAVHPFTGIIMFIAFIIMALIYWHHNIPEKNDIRWAKGIVEVL
                     KGNEHKVSDNGKYNLGQKLLFWTLILAMFTLLATGIIMWRQFFSHYFSIPMLRIAILL
                     HSASAFMLFTGILVHMYMAFWVKGSIRGMVEGWVTVRWAKKHHPRWYREEVLPELERQ
                     VAEQNNK"
     gene            4809..5726
                     /gene="fdhE"
                     /locus_tag="PXD66_001830"
     CDS             4809..5726
                     /gene="fdhE"
                     /locus_tag="PXD66_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HDL6320105.1"
                     /translation="MSIKILPQDDIKQAASSFQQPELLFANPKNLYLRRAKRLRELAK
                     QNPFGEYMEFSANLVDIQLALLESQPIADYSQKITACVERTQGETPLNAQNFQRTDEW
                     RTLLLAIIEKFKPYANDTVLATIEVLEKAAKSELDTLADNLLNERYELVGADKAVFIW
                     AALSLYWVQLAQQLPRNSRAEIGHKHLCPVCNSAPVSSVIHFGEAQGLRYLHCSLCES
                     EWHVVRTKCTNCDETGKLDYWSLDTVEAAVKAESCGDCHSYLKVLYQDKDVNVEPVAD
                     DLATLFLDSEMEQKGLSRSGINPFLFQVE"
BASE COUNT         1689 a         1249 c         1344 g         1483 t
ORIGIN      
        1 tagggggaaa atgatacacc tcttagatca atttattaaa ttaattttga tcaaattttg
       61 ttttatttct cacattatgt gatttggagt gattatgcag atctcaagac gtaagttctt
      121 caaggtctgt gcaggtggta tggcgggaac ttctgctgca atgttgggtt ttgctccagc
      181 aacagcatta gctgcaccgc gtgagtataa gctcttaaga gcaaaagaaa ctcgacaaac
      241 ctgtacttac tgcgcggtag gctgtggcat gattatgtac agcttaggcg gcgaagcaat
      301 gaacagcaaa ggaaagttaa ttcatattga gggtgattct gaccatccag taagccgtgg
      361 ggcgttatgt ccgaaaggtg caggggcgtt ggactatgtt aatagtgacc gccgtgtgca
      421 ttatccggaa gtgcgtgaag ccggttcaag tgagtggaaa cgcatttctt ggcacgaggc
      481 aattgaacgt attgcccgcc atatgaaaaa tgaccgtgat gagaacttcg tcacccacaa
      541 tgaagcaggg ctggaagtaa accgttggat gacggcaggt ttcttggcag gctcggcgtg
      601 tagtaacgaa accggtatcg tcacccagaa gtttatccgc tctctcggta tgatttttac
      661 cgacaaccaa gcgagtatct gacacggacc aacggtagca agtcttgctc catcatttgg
      721 tcgcggtgcg atgaccaacc actgggttga tattaaaaac tccgatttag tgatcgttat
      781 gggcggtaac gcagccgaag ctcaccctgt gggtttccgt tgggcagttg aagcgaaaaa
      841 acagaacggt gcgaagttaa tggtggtaga tccacgcttt aaccgtagtg ctgcggtggc
      901 ggatatttat ataccattgc gtccgggtac ggacattgcg ttcttgttag gtgcggtaaa
      961 ctacctgctt aaaaacgaca aaattcaaca cgagtatgtg aagcattaca ccaatgccac
     1021 attcttactc aacgaagggt ttaaattcga agacgggctt ttcaccggct acgatgaagc
     1081 cactcgtacc tataaagaca aatccacttg ggcttatcag ttagatgaac agggtcagcc
     1141 gaaacgggat atgacaatgc aagatccacg ttgtgtgatc aatatgctca aagaccacgt
     1201 ttcccgctat acgcctgaaa tggtagaacg cattaccggt acgccacaaa aagatttcct
     1261 gattttctgt gaagagattg cgaaaacctc agcgccggat aaagccgcca ccttcctcta
     1321 tgccttaggt tggacacaac acaccgtggg cgcacaaaac attcgctcaa tggcgatcat
     1381 tcagttgctg tgcggtaaca ttggtgtctc cggcggtggg gtaaacgcac tgcgtggtca
     1441 ctctaatgta caagggatta ccgacctagg cttgttccca catatgttgc cgggctatat
     1501 ccgcctgcca accgaagcgg acgcaacctt agaaagctac ttaaatgcga ttgcaccgaa
     1561 aaccttagtg gcagggcaag taaactacgg gcaaaacaac ccgaaattcg ctgtgagtat
     1621 gctcaaaacc ttctacggcg ataaggcaac caaagagaac gagttcggct atcactattt
     1681 accgaaatac ccgaaaggcg gtacagatca cttccgcttt atcgaacaga tgtatcaagg
     1741 caaagtgaac ggctatttct gccaaggtat gaacccgatt gcctcttacc ctaactcgca
     1801 gaagttaatt aaagcgttga gcaatttgaa atatttagtc atttttgacc cgcttattac
     1861 cgatacttcc gagttctgga aaaatcacgg tgagttcaac aatgtgaacc cgtctgaaat
     1921 tcaaaccgaa gtgttccgct tgccgaccac ttgctttgcg gaagaagatg gttcaattgc
     1981 gaactccggt cgctggttac aatggcactg gaaagcagca gacgcaccac acgaggcaaa
     2041 accggacgtg gatattctgt ctgaaatccg tgccgaactg atccaccttt atcataaaga
     2101 aggcggaaaa gcaccgcttg aaccgttaga agcgatgacg tggaactata tgaacccgtt
     2161 agaaccgaag ccggaagaga tcgccaaaga gaacaacggt tatgcattgg aagatctgaa
     2221 agatgcagac ggcaacatca tcgtgaaaaa aggcgaacta ttatccagct ttacacaaat
     2281 gcgtgatgac ggtacaacct ccggtggttg ttggatctac tgcggtcaat ggactccgaa
     2341 aggtaaccaa atggcgaacc gtgataactc cgacccatca ggcttaggta acaccttagg
     2401 ctgggcattc tcgtggccgg taaaccgccg tattctatac aaccgtgcat cggcagattt
     2461 aagcggtaaa ccgtggaacc cgaaacgcca gctagttaaa tggaacggta aaaactggaa
     2521 ctatgtggac gttgccgatt acggtacagc accaccaaat agccctgtga caccatttat
     2581 tatgcaaccg gaaggggtat ccggcttgtt cgtgcgtgaa cgtatgcaag acggtccatt
     2641 ccctgagcat tatgaaccgt ttgaaacgcc aatcggcacg aacccgttac acccgaatgt
     2701 aatcagcagt ccggtggcac gcattctgcc gagcgataaa gaggatttag gcaccagcga
     2761 tgagttccca tatgtcggta caacttaccg cttaaccgaa cacttccact actggacgaa
     2821 aaatgccctg attaacgtca ttgctcaacc ggagcaattc gtggaaatcg gcgagaaact
     2881 tgccgaagag aaaggcatta aaaatggcga caaagtacgt gtttggtcta aacgtggcga
     2941 aattaaagcc gttgccgtgg tgactaaacg tatccgttca ttggtctctg acggcaaacc
     3001 aatccacact atcggtgtgc cgatccactg gggctttgcg gctacaaccg gtgcgaaaaa
     3061 aggctatttt gccaataact taaccgtgcg aacaggtgaa ccaaactcaa atacacctga
     3121 atcgaaatgt atgttattaa acattgagaa aattgtgggg gcttaacaat gagtacagtt
     3181 caaactcaaa acattattaa aatctccgca acctcggcgc tgacaccggc gccgcaggct
     3241 cgttcgcacc aagtggaagt agcgaaactg atcgatgtca ccacttgtat cggctgtaaa
     3301 gcctgtcaag tgggctgttc agaatggaat gacattcgct ccgatgtaaa tgcaaactgt
     3361 gtcggcgtgt acgacaaccc tgcggatcta aatgcgaaag cgtggacagt aatgcgcttt
     3421 aacgaagtag aagaaaacga ccgtttagaa tggttgatcc gtaaagacgg ctgtatgcac
     3481 tgtgccgagc cgggttgctt gaaatcttgc ccgtcaccgg gtgcgattat ccaatacgcc
     3541 aacggtattg ttgatttcca atccgacaaa tgtatcggtt gtggctactg tatcgcaggt
     3601 tgtccgttca acattcctcg tatgaatccg gacgataacc gtgtttacaa atgcaccctg
     3661 tgtgtggatc gtgtcaatgt tggtcaagaa ccggcttgcg tgaaaacctg cccgaccggt
     3721 gcaatccgct tcggtagcaa agaagagatg aagttctatg cagaacaacg cattgctgat
     3781 ctgaaatctc gtggctatga aaatgccggc ttgtacgacc ctgaaggcgt tggcggtacg
     3841 cacgtaatgt atgtattaca tcatgccgac aaaccggagc tttattcagg cttaccgaaa
     3901 gatccgaaga ttgataccac agtaacctta tggaaagacg ttttaaaacc tgtggcggca
     3961 gtagcgttgg gtggtttagc tttagcagaa gttgcacact atattaccgt tgggccaaac
     4021 gttgaggaag atcttgaaga acaccaccat agcgaaaatg cagaaggagg caaacatgag
     4081 taagaaattt gaaatcacga acgataccaa aatcgttcgt cataagccct tagctcgtgt
     4141 tagccactgg ttcttagtaa tctctttttt tatgacgatg tttaccggtg ttgccttctt
     4201 cttccctgac tttgcttggc ttaccgagat tttaggtact ccacagattg cccgtgcagt
     4261 ccacccattt accgggatta taatgtttat tgcatttatt attatggcgt taatatattg
     4321 gcaccataat atccctgaga aaaatgatat tcgttgggca aaaggcattg ttgaagtact
     4381 aaaaggtaat gagcataagg tttccgataa tggaaaatac aatttgggtc aaaaattact
     4441 cttttggact cttattcttg cgatgttcac tcttttagcc acagggatta ttatgtggag
     4501 acaatttttc tctcactact tctcgattcc tatgctaaga attgcaattt tacttcattc
     4561 agcaagtgca tttatgctat ttaccggaat tttagtacat atgtatatgg cattctgggt
     4621 aaaaggctca atcagaggta tggttgaggg ttgggttacc gttcgctggg caaaaaaaca
     4681 ccacccaaga tggtaccgtg aagaagtgct acctgaatta gaaagacaag tagctgagca
     4741 aaataataaa taacctcaag tgcggtatta ttaccgcact tttattttat tggtttaaaa
     4801 agaatattat gagtatcaaa atactacctc aagatgatat caaacaagca gctagttcat
     4861 ttcagcaacc cgagctctta tttgctaatc ccaaaaatct gtatttacgc cgcgctaagc
     4921 ggctacgtga attagccaag caaaacccat tcggtgaata tatggagttt tctgctaacc
     4981 tcgtggacat tcaattagca ttacttgaaa gccagccaat tgcagattat tctcaaaaaa
     5041 taaccgcttg tgtagagcgc acgcaaggag aaacaccgct aaatgcacag aattttcagc
     5101 gtactgatga atggcggaca ctactcttag ccattattga gaaattcaag ccttatgcaa
     5161 atgatactgt tcttgcaaca attgaagtat tggaaaaggc agctaagtca gagctagaca
     5221 cacttgctga taatttgcta aatgaacgat acgaacttgt gggcgcagat aaagctgtct
     5281 ttatttgggc tgctctctca ctttactggg tacaattagc tcaacaatta ccacgtaatt
     5341 ctcgtgcaga aataggccat aaacatttat gtccggtatg taactcagca cctgtaagca
     5401 gtgtgattca tttcggtgaa gctcaagggt tacgttatct acactgttct ttatgtgaaa
     5461 gtgaatggca tgtagtacgt acaaagtgta caaactgtga tgaaaccggc aaactggatt
     5521 attggagctt agatacagta gaagcagcag ttaaagctga aagttgtggc gactgccatt
     5581 cctacttaaa agtactctat caagataaag acgtgaatgt ggaacctgtt gctgatgatt
     5641 tagcaactct attcttagat tccgagatgg aacaaaaagg tctttcaaga agtgggataa
     5701 atccattttt atttcaagta gagtaactca tttacaacaa aaacggagca taacgctccg
     5761 ttttt
//
LOCUS       DANLXF010000150         5731 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.150, whole genome shotgun sequence.
ACCESSION   DANLXF010000150 DANLXF010000000
VERSION     DANLXF010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5731
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.150"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            80..1951
                     /gene="rpoD"
                     /locus_tag="PXD66_001831"
     CDS             80..1951
                     /gene="rpoD"
                     /locus_tag="PXD66_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDL6320106.1"
                     /translation="MENQADQQLEQQSQLELLISQGREQGYLTLSEVHDHLPEELVDA
                     EQIEDIIQMINDMGIQVLEAAPDADDLLLAENIADEDVVEEATKVLSTVESELGRTTD
                     PVRMYMREMGTVELLTREGEIDIAKRIEEGINEVQCAIAEYPEALSGLLACYAQVETG
                     EMRLSDLVTAFVDPNILEEAPLEDIEAALGEDEESSDVPDVDDENEDENGDSSSDSSD
                     DSSIDPELAREKFEALRSQHEKVLLVIQKHGRTSKKSKEQIEILSHIFREFRLVPKQF
                     DLLVASMQKLMKQVRTEERQIQRYAVEYAGMKKADFLKAFQGHELSEAWIEKAINAKK
                     NAAPKLANYVDNIRVNIANLQNLEQTSGLTIAQIREIGGRISNGELKARRAKKEMVEA
                     NLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAIT
                     RSIADQARTIRIPVHMIETINKLNRISRQCLQEMGREATPEELAERMGMPEDKIRKVL
                     KIAKEPISMETPIGDDDDSHLGDFIQDESLELPLDSATAESLRMATNEVLEGLTPREA
                     KVLRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSETLRSFLDD"
     gene            2045..3031
                     /locus_tag="PXD66_001832"
     CDS             2045..3031
                     /locus_tag="PXD66_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDL6320107.1"
                     /translation="MKKSLLALLIGSVFLISACEDGKMTQTVLEAEKQIVQLGTELKN
                     AQADLAKKEAEVAELSGVKAENEKLKSELENAQQNTALNVEIVKIFDKKEVIKHQVDP
                     KQEFAIEESTVSAFVSLPKTNFDWLNQLLINQAYDYNEEQGRKLKNPTEEEFKKHREA
                     VYQGLVESVKTEPAIGYDDHISSAFIGQRNNIATFTMMHYTYMGGAHGMHYTQYINVD
                     LAKKAIIGLNDLISPKNQVQLKDILWENYSSVRVDENGNYNGFADKKEFRISDQFYFT
                     PHGIAFVYPPYELGAYAEGDIEVEADWYIINKLLNSEYQRGEKDGFFEELDN"
     gene            complement(3107..4015)
                     /locus_tag="PXD66_001833"
     CDS             complement(3107..4015)
                     /locus_tag="PXD66_001833"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDL6320108.1"
                     /translation="MNKNSLFSIWFTTARPKTLPLALASIIVGSALAHWAGKFDVVTT
                     LLAFITTILLQILSNFANDYGDHVKGSDTKERIGPLRAIQHGVIGGEQLKKAVIILSL
                     LSFVSGVALSAYAFETVQDLFFFIGLGIVSIIAAITYTVGKKAYGYLGLGDLFVLIFF
                     GFVAVIGVFYLQAHSLPIMILLPAFGCGLLSVAVLNINNLRDINQDRQAGKNTLIVRI
                     GSQNGRKYHVALLVLAVISYLIFAISEFEHWYSFIFLLSVPLLAKHARFVYQHKDPKE
                     LYPILGQMAGLALLTNLLFSLGIVLG"
     gene            4195..4932
                     /gene="tsaA"
                     /locus_tag="PXD66_001834"
     CDS             4195..4932
                     /gene="tsaA"
                     /locus_tag="PXD66_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HDL6320109.1"
                     /translation="MNSTSNQLTPIQLNPIGFVESPYDEKFSVPRQPNLVAEGKGILR
                     LIPPYNTPDAVRGLEKFSHIWLIFQFHHIPEREWHATVRPPRLGGNERVGVFASRATH
                     RPNPLGLSKVKLEAVVAGGSEVYLKLGSVDLVNGTPIFDIKPYIAFADSEPQAVSGFA
                     QQKPQARLAVRFSEEAKQAVEFCRNFAKFGIEQPLSFLADVIAQDPRPAYQQGKPSER
                     VYGMNLAGYNIRWQIENDTQAIVLSIE"
     gene            4990..5709
                     /locus_tag="PXD66_001835"
     CDS             4990..5709
                     /locus_tag="PXD66_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253715.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HDL6320110.1"
                     /translation="MKLKNFLTILPLAVATAVTSAETHTVEHKDKPASFNFSTEVSRT
                     VEKDVMQASVYSQKSGNSLPELKKFVSENLNRVLAEAKKHPSIEVQAEGVTNHVNYNN
                     KGNIDGWDTSGSIQLKSKDFDAMAAVLENLGKDVAIRYIDFSVSPEKMASLEDEMTLE
                     IISKFQHKADVIQKALGAKTYKLVNINIQTPNDGGRPYYEHRAMYAVSSMAQSKSMDE
                     IPLEAGKTTISATASGSVEFE"
BASE COUNT         1856 a         1082 c         1295 g         1498 t
ORIGIN      
        1 tttgtgaatt tatgaagtcg aaccaaattt agtggtataa tccactacta tttttgctcg
       61 cataataaac ggatagacta tggaaaacca agcagatcaa caattagaac agcaatctca
      121 attagaactt ctgatcagtc aagggcgtga acagggatat ttaacgcttt ctgaagtgca
      181 cgaccactta ccggaagagt tggtggatgc ggagcagatc gaagatatta ttcagatgat
      241 caacgatatg ggtatccaag ttttagaggc tgcccctgat gcggatgatt tactactcgc
      301 tgaaaatatc gccgatgaag atgtggtaga agaagccacc aaagtgcttt caaccgtaga
      361 atcagagtta ggtagaacta ctgaccctgt gcgtatgtat atgcgtgaga tggggacagt
      421 tgagctttta acccgtgaag gtgagattga tatcgctaaa cgcattgaag aagggattaa
      481 cgaagttcaa tgtgcgattg cggaatatcc ggaagcctta agtggcctgt tagcctgcta
      541 tgctcaagtc gaaaccggtg aaatgcgcct ttctgattta gtgacagcat ttgtcgatcc
      601 taatatttta gaagaagcac cgttagagga tattgaggct gctttaggtg aagatgaaga
      661 atcttctgat gtgcctgatg tagatgacga aaacgaagat gaaaacggtg atagcagttc
      721 agacagcagt gatgacagct caattgaccc ggaacttgcc cgtgaaaaat ttgaagccct
      781 acgcagccaa catgaaaaag tgttattggt cattcaaaaa cacgggcgaa ccagtaaaaa
      841 atcgaaagag caaattgaaa ttttatctca tattttccgt gaattccgtt tggtgccgaa
      901 gcaatttgac ttattggttg cttctatgca aaaactgatg aagcaggttc gcactgaaga
      961 acgtcaaatt caacgctatg cagtggaata tgccggcatg aaaaaagcgg atttcttgaa
     1021 agcattccaa gggcacgaat taagcgaagc gtggattgaa aaagcgatta atgccaagaa
     1081 aaatgcagca ccgaaattag ctaattatgt ggataatatc cgtgtcaata ttgctaactt
     1141 acagaattta gagcaaacat caggcttaac cattgctcaa attcgtgaaa ttggcgggcg
     1201 tatttccaat ggtgagttaa aagcccgccg tgcgaaaaaa gagatggtgg aagctaactt
     1261 acgtttggtg atttcgattg cgaaaaaata cactaaccgt ggcttgcagt tcttagattt
     1321 aatccaagaa ggaaacatcg gtttaatgaa agcggtggat aaatttgaat accgccgtgg
     1381 ttataaattc tcgacttatg caacttggtg gattcgtcaa gcgattaccc gttcgattgc
     1441 tgaccaagcc cgtacaattc gtattccggt gcatatgatt gaaactatca acaaactcaa
     1501 ccgtatttca cgccaatgtt tacaagaaat ggggcgtgag gcaacgccgg aagaactggc
     1561 agaacgtatg ggaatgccgg aagataaaat ccgcaaagta ttgaaaattg ccaaagagcc
     1621 gatttcaatg gaaacaccaa tcggtgacga tgacgattca catttaggtg atttcattca
     1681 agacgaaagc cttgaattac cattggattc cgccactgcg gagagcctgc gtatggcaac
     1741 gaacgaagtg ttagagggct taaccccgcg tgaagcgaaa gtgttgcgta tgcgttttgg
     1801 tattgatatg aacacagacc acacactcga agaagttggt aaacaatttg acgtaactcg
     1861 tgaacgtatc cgccaaattg aggcgaaagc actgcgcaaa cttcgccacc cgagccgttc
     1921 ggaaacctta cgcagtttct tagacgacta atcatttccc ctatgcctga tggtataggg
     1981 gattttgttt taatctgtat aacaagcggt gatatttacc gtatttttta caaaagaggg
     2041 agagatgaaa aaatcactat tagcactttt aattggctca gtatttctga tttcagcttg
     2101 tgaagacggc aaaatgaccc aaacggtatt agaagccgaa aaacaaattg ttcagttagg
     2161 cacagaatta aaaaatgctc aggctgattt agcaaaaaaa gaggctgaag ttgcagaact
     2221 ttcaggggtg aaagcagaaa atgaaaaatt aaaatcagag ttagagaacg ctcagcaaaa
     2281 cacagcgtta aatgttgaaa ttgtgaaaat ctttgataag aaagaggtga ttaagcacca
     2341 agttgatccg aaacaagaat ttgcaataga agaaagtaca gtaagtgcgt ttgtgtctct
     2401 accaaaaacc aattttgatt ggcttaacca gcttttgatt aatcaggcct atgattacaa
     2461 tgaagaacaa ggcagaaagc tgaaaaatcc aaccgaagaa gagtttaaaa agcatcgtga
     2521 agcggtttat caagggttgg tagaaagtgt gaaaaccgag ccagctattg gctatgatga
     2581 tcatatttcc agtgctttta tcgggcaacg caataatatt gctacattca ctatgatgca
     2641 ttacacctat atgggtgggg ctcacggtat gcactatacg caatatatca atgtagattt
     2701 agctaaaaaa gcaatcattg gcttaaatga tttaatcagc ccaaaaaatc aagttcaact
     2761 gaaagacatt ctttgggaaa attacagtag cgtgcgtgtg gatgaaaatg gcaactataa
     2821 tggatttgct gataaaaaag agttccgtat ttctgaccaa ttctacttta cccctcacgg
     2881 cattgctttt gtttatccgc catacgaatt aggtgcttat gcagaaggcg atatagaggt
     2941 tgaagcggat tggtacatta ttaataagtt gctcaattcg gagtatcaac gtggcgaaaa
     3001 agatggattc tttgaagaac tagataacta attgtaaatt tagataaaaa acaaccgctt
     3061 gtaatccttt attacaagcg gtttagtttt gttaaaattt tgcaacttac cctaacacaa
     3121 tgcccaaact aaataataga ttagtgagca acgccagccc tgccatttgc cctagaattg
     3181 ggtagagttc ttttgggtct ttatgctgat agacaaaacg agcgtgtttt gccagtaacg
     3241 gtacagacag caaaaaaatg aagctatacc aatgttcaaa ttcagaaatt gcaaaaatca
     3301 gataagaaat gaccgctagc actaacaatg caacgtggta ttttcgcccg ttttggctac
     3361 cgattcgtac aatgagagtg tttttcccag cttgtcggtc ttggttaata tcacgcaagt
     3421 tattgatatt taacactgct acagacagca atccgcaacc gaaagcaggg agcagaatca
     3481 ttataggtag gctatgagcc tgcaagtaga acacgccgat taccgctaca aagccaaaga
     3541 aaatcagcac aaatagatcg cccagcccca aatagccgta ggcttttttg ccgacggtgt
     3601 aagtaatggc ggcaataatt gagacgatgc caagcccgat aaagaaaaac agatcctgca
     3661 ctgtttcaaa agcataggca cttaaggcaa caccggaaac aaacgagagt aggcttaaga
     3721 taatcacggc tttcttaagt tgttcaccgc caataacacc gtgttgaatc gcccgtagcg
     3781 gtccgattcg ttctttggtg tcagagcctt tgacgtggtc gccgtaatca ttggcgaagt
     3841 tagacaggat ctgcaacaaa atggtggtga taaatgcgag taaggtggta acaacatcaa
     3901 atttccctgc ccaatgagca agtgctgaac ccacaataat tgaagctaac gccagcggta
     3961 aagttttcgg gcgagcggtg gtaaaccaaa tcgaaaagag agaatttttg ttcatagaga
     4021 ataatcttag cgtaatttca tttacaatga tgttaaggat ttaagcgata gtgcaagcgt
     4081 ccactcttgt gttaaatggt aaaacgaatt ggtacaagcg tggacgcttg caccatcctc
     4141 aatttcatat cattatgtcc aatattttat attattttaa cagaaaaaag cgtaatgaac
     4201 tctacctcaa atcaactaac cccgattcaa ttaaacccta tcggttttgt ggaaagcccc
     4261 tatgatgaaa aattttccgt gcctcgccag ccgaatttgg tagcggaggg gaaaggcatt
     4321 ctacgtttaa ttccgcctta caataccccc gatgcagtca gaggcttgga gaaatttagt
     4381 catatttggt tgattttcca attccatcac attcccgaaa gagagtggca cgccaccgtc
     4441 agaccgcctc gcttgggcgg gaacgaacgg gtcggggtct ttgccagccg tgccacccat
     4501 cgtccgaatc cgcttggctt atcgaaagtg aagttagaag cggtagtggc gggggggagt
     4561 gaagtttatt tgaaattagg cagcgtggat ttagttaacg gcacaccaat ttttgacatc
     4621 aagccttaca ttgcatttgc agatagcgag ccgcaagcgg tttccggttt tgctcagcaa
     4681 aaaccacaag caagattagc ggtgcgtttt agcgaggagg caaaacaagc tgtcgaattt
     4741 tgccgaaatt ttgcaaaatt tggcatcgaa cagccgctta gttttcttgc cgatgtcatc
     4801 gcccaagacc ctcgcccggc gtatcaacaa gggaaaccta gtgagcgagt ttatggtatg
     4861 aaccttgccg gctacaatat tcgctggcaa attgaaaatg atacacaggc gattgtgctt
     4921 tcgattgaat gaactttcct acgcaagtga gatctaagaa atgattttta acccttttaa
     4981 ggactgatta tgaaattgaa aaattttctt accattctac cgcttgcagt cgcaacagcg
     5041 gtaacttcag cggaaactca taccgtggaa cataaagata aaccggcttc ttttaatttc
     5101 tcaaccgaag tctctcgcac ggtggaaaag gacgtgatgc aggcaagtgt gtatagccaa
     5161 aaatcaggta actccttgcc ggagctgaaa aaatttgtat ctgaaaacct gaatcgtgta
     5221 ttggctgagg caaaaaaaca tccatcgatt gaagtacaag ctgaaggtgt gactaatcac
     5281 gttaattata acaacaaagg gaatattgat ggttgggata cctctggtag cattcagttg
     5341 aaaagcaaag attttgatgc aatggcagcg gtgttggaaa atttaggtaa agatgtggcg
     5401 attcgttaca ttgattttag tgtatcgcca gagaaaatgg cgtcccttga agatgaaatg
     5461 acgctggaaa ttatcagcaa atttcagcat aaagccgatg tgattcaaaa agccttaggg
     5521 gcgaaaacat ataagctagt taatatcaat attcaaactc caaatgatgg tggtagacca
     5581 tattatgaac acagagcaat gtatgcagtt tcatcaatgg cacaaagcaa atctatggat
     5641 gaaatcccgt tagaagcagg taaaaccaca atttcagcta ccgcttcagg aagtgtggaa
     5701 tttgaataga aatgtataga tttgtataga g
//
LOCUS       DANLXF010000151         5628 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.151, whole genome shotgun sequence.
ACCESSION   DANLXF010000151 DANLXF010000000
VERSION     DANLXF010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5628
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.151"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            54..323
                     /gene="rpsO"
                     /locus_tag="PXD66_001836"
     CDS             54..323
                     /gene="rpsO"
                     /locus_tag="PXD66_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDL6320111.1"
                     /translation="MSLSVEAKAKIVAEFGRDAKDTGSSEVQIALLTAQINHLQAHFA
                     EHKKDHHGRRGLLRMVSRRRKLLDYLKRTDLAKYSETIARLGLRR"
     gene            395..1264
                     /locus_tag="PXD66_001837"
     CDS             395..1264
                     /locus_tag="PXD66_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HDL6320112.1"
                     /translation="MAFASLFTLLDDIAAVLDDVSIMTKVAAKKTAGVIGDDLALNAN
                     QVSGKNVVPERELPIVWAVAKGSLINKVILIPLALLLSVFLPQAIIPLLMIGGAYLCF
                     EGVEKLLHRLLHKNNQAHSDETETLSEKDKIKGAIRTDFILSAEIIIIALGVLQDSST
                     LTQILSLSAIGVGITLFVYGLVGLIVKLDDIGLWLMQKKSTFSQAVGKGLLFAMPWFM
                     KGLSVIGTIAMFLVGGGIFTHNLPMIHHFVEQFGISPHFSTLVDFAVGIVVGIIACLI
                     ILPLMKLFSKKAH"
     gene            complement(1354..2619)
                     /locus_tag="PXD66_001838"
     CDS             complement(1354..2619)
                     /locus_tag="PXD66_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HDL6320113.1"
                     /translation="MFRTLFLMLLLLVGLISGPYLAGNQGYVRIETATTVIEMSIVML
                     VVFFVLAMAVVYLLEWIVTKICRMSNGAYNWFSTRKHKKAQQETLEGLMRMSEGNYSK
                     AEKLFSKNAKHADEPVLNLIKAAEAAQQNGDDLAANKYLIKATEIAGPNNVAVELVRT
                     RILLQQGKLPAARTAIDSLLELAPHSDEVLRLAIQIYKESRAYVALDRLLVEIGQRNF
                     LTAEEYAELEYFVDDGLQDERLQEEGQEGLLSWWENQPNRRRKSVYSRVGVIKRLIDS
                     DDHQSAQEIALETLKKFEDEQLQGLFVQLTRLQIDSDSKLLKVLAKRADKANVKYTDD
                     YARALGYIYTRDGQYQKAKSQFELLLAHDECVADDRIMALHVAEQMGDTLMANRIREE
                     NLKQVNMATQSKVNEPLALPQTATESNSL"
     gene            complement(2638..3948)
                     /locus_tag="PXD66_001839"
     CDS             complement(2638..3948)
                     /locus_tag="PXD66_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDL6320114.1"
                     /translation="MSRSKKVTEHLEHSAEKVVEEAQQAVDSAGSFAKKEIEEDVVNE
                     AIEKDIQTEMQKAPKEQVVVQKSGGKGLALVALLVALAVGGAGHYMANKKFAEVESQI
                     QAVSAKANQPTTQTMVEMPNFENEKAQIAELVTEYQKALERIKALETAQNGYTQQISS
                     LQLQLQKLNNTSGSDNTFWLLSEADFLLNNAARKVVLDNDIETAKNLLLEADQVLSKA
                     PNTIHIREAIKTDLNTLTALHQIDQNALMQRVANLANKLDDLPILESEQASNSKQDGQ
                     VSDSLADWEKNLEKSAASFLDHFIRVSKRNAADEKAFVAPNQEIYLRENIRLRLQIAI
                     LAVPRQQSELYKKSLQTVGSWVRSYFETSNENVQHFLKELDELAEQTIYVDAPTSLQS
                     LKVIAQQLNKTPEKIEKVEIKAEKELEQAEPVKAEQDAMPLSAQ"
     gene            complement(3960..4724)
                     /locus_tag="PXD66_001840"
     CDS             complement(3960..4724)
                     /locus_tag="PXD66_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HDL6320115.1"
                     /translation="MNVLVTRLDSRGQELVDMLNEQQIFAIHQPLFTIEAGKELPLLP
                     SAISRLKSDDYVFVVSPNAVDYAAKTLVDTGFHFRSDLKYFAVGQRTAKYLTEKAEQA
                     VIYPIEFENSEGVLALPEMQNLTDKTILILRADSGRELLAETAVLRGATIQYLECYRR
                     EPVTDDIPEKISLCKRLGVDTIVITSSEILRSLYEQAKADCRAWLFECDLVVVSRRIA
                     KIAKQMGWQEDKIRVSDKADNVTLMNTLLFKVGKSN"
     gene            complement(4724..>5628)
                     /gene="hemC"
                     /locus_tag="PXD66_001841"
     CDS             complement(4724..>5628)
                     /gene="hemC"
                     /locus_tag="PXD66_001841"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDL6320116.1"
                     /translation="SPLALWQANFVKSELEKHFPQLSVELVTMVTKGDIILDTPLAKI
                     GGKGLFVKELELALLENRADIAVHSMKDVPMSFPEGLGLAVICEREDPRDAFVSNNYH
                     SLEDLPKGAVVGTSSLRRQCQLMAKYPHLEVKSLRGNVGTRLSKLDNGEYDAIILASA
                     GLIRLGLQERIRSYISVEQSLPAAGQGAVGIETRANDERILNYIAKLNHSSTACCVMA
                     ERAMNTRLQGGCQVPIGGYATLEGDELTLNALVGSLDGSQIIRASAKGHKQEAEQLGI
                     LVAEQLLAQGADKILAAVYNENVQ"
BASE COUNT         1589 a         1233 c          956 g         1850 t
ORIGIN      
        1 agttagagat cggctattga tgtattatca acccttaaca aaaggaaatt aaaatgtctc
       61 taagcgtaga agcaaaagca aaaatcgttg ctgaatttgg tcgtgatgca aaagacactg
      121 gttcttctga agttcaaatt gcattattaa ctgcacaaat caaccactta caagcacact
      181 ttgctgagca taaaaaagat caccacggtc gtcgtggttt attacgtatg gtttcacgcc
      241 gtcgtaaatt attagactac ttaaaacgta ctgatcttgc taaatattcg gaaactatcg
      301 cacgtttagg tttacgtcgc taatttcaaa tattagatat gagagccgag ctgaaaagtt
      361 cggctttttt ataaatttta ttcgaggaaa aaatatggct tttgcgtcat tatttacatt
      421 attagacgat attgctgccg tgcttgatga tgtatcgatc atgactaaag tggctgctaa
      481 gaaaacagca ggcgtaatcg gtgatgatct tgccttaaat gctaatcaag tcagtggaaa
      541 gaatgtggtg ccagaacgag aactaccgat tgtttgggct gtagcgaaag gctcattaat
      601 caataaagtg atcttgattc cactagcgtt gctgctatcg gtctttttac cgcaggctat
      661 tatcccgcta ttaatgatag gcggtgcata tttatgtttt gaaggcgtag agaaattact
      721 ccatcgtcta ttacataaaa acaatcaagc tcattctgat gaaaccgaaa ccctttctga
      781 aaaagataaa attaaaggtg caattcgcac cgattttatt ctatcagcag agattatcat
      841 tattgcgtta ggtgttttgc aagactcttc aaccctaacc caaattttat ctctctccgc
      901 tatcggggtc ggaattaccc tgtttgttta tggcttagtt ggattaattg taaaactaga
      961 tgatatcggt ttatggttaa tgcagaaaaa aagcacattc tcacaagcgg ttggaaaagg
     1021 cttattattt gcaatgccgt ggtttatgaa aggactttct gtgattggta ctattgcgat
     1081 gttcttagtc ggtggtggta tttttaccca taatttacca atgattcatc acttcgtaga
     1141 acaattcggt atctctcccc atttttctac tctggttgat tttgcagtgg gtattgtggt
     1201 tggtattatt gcatgcttaa ttattttacc tttgatgaaa ttattttcta aaaaggcgca
     1261 ctaatcccca aaagcgtagc tttggttaaa gctagctttt tatctcttgc tataaaaaaa
     1321 tagcaggttt caagcctgct atttttttaa ttattataat gagttagact cagtggctgt
     1381 ttgcggtaag gctaatggct cattaacttt agactgagta gccatattca cctgtttgag
     1441 attttcctca cgaatacggt ttgccattaa tgtatccccc atctgctctg ccacgtgcag
     1501 tgccataata cgatcatcag caacacattc atcatgagcc aataacaatt caaactgaga
     1561 cttcgctttt tggtattgcc catctcgtgt ataaatatag ccaagcgctc tagcataatc
     1621 atcggtatat ttaacattcg ctttatctgc ccgttttgct aatactttta gtagcttact
     1681 gtcagaatca atttgtaaac gggttaattg cacaaataaa ccttgtaatt gctcatcttc
     1741 aaatttcttc aacgtttcta aagcaatttc ttgtgcagat tgatgatcgt cactatcaat
     1801 taaacgcttg atgacaccga cacgactata caccgattta cgacgacgat ttggttggtt
     1861 ttcccaccaa gaaagcaagc cttcctgccc ttcttcctgt aaacgctcat cttgtaaacc
     1921 atcatcaacg aaatactcta actcagcata ctcttctgca gttaagaaat tacgttggcc
     1981 gatttcaact aataatctat ctaacgcaac ataagctctg gattctttat aaatttgaat
     2041 cgctaagcgt aatacctcat cgctatgagg tgcaagctct agtaggctat caatcgcagt
     2101 tcgggcagcc ggtaattttc cttgttgtaa taaaattcgg gttcgtacca actccactgc
     2161 aacattattc ggtccagcta tttcagttgc cttaattaaa tatttatttg cagctaaatc
     2221 atcaccattt tgctgagcag cttccgccgc tttaattaaa ttcagtacag gctcatctgc
     2281 atgttttgca tttttactaa agagtttttc cgcttttgag taattccctt cactcatacg
     2341 cattaaacct tccagtgttt cttgctgagc ttttttatgc ttacgagttg agaaccagtt
     2401 ataagctcca ttactcatac ggcatatttt agtgacaatc cattcaagta aataaactac
     2461 cgccattgcc aacacaaaaa aaaccacaag cattacaatg ctcatttcaa tcacggtagt
     2521 tgctgtttca attctgacat aaccttgatt tccggctaaa taaggcccag agattagtcc
     2581 aactaataat aaaagcatca aaaagagcgt tctaaacata atctattctc ctttttatta
     2641 ttgtgctgaa agaggcatcg catcttgctc tgcttttaca ggctctgctt gctctaattc
     2701 tttttcagct tttatttcga ctttctctat tttttcaggg gttttattaa gttgctgagc
     2761 aatcaccttt aagctttgta atgaagttgg agcatccaca taaatagttt gttctgccaa
     2821 ctcatctagc tctttcaaga aatgttgcac attttcattt gaagtttcaa aataacttct
     2881 tacccaagag cctaccgttt gtaacgattt tttataaagt tcgctttgct ggcgaggaac
     2941 agctaaaatc gcaatttgta aacgcaagcg aatgttttca cgcaaataaa tttcttgatt
     3001 cggtgcaaca aaggctttct catctgctgc attacgtttc gatacacgaa taaaatgatc
     3061 taaaaatgaa gcagcacttt tttctaaatt tttctcccaa tccgcaagag aatcacttac
     3121 ttgcccatct tgtttgctat ttgaagcttg ttcactttcc aaaatcggca aatcatctaa
     3181 tttattcgct aaatttgcga cacgctgcat taaggcattt tgatcgatct gatgcaatgc
     3241 ggttaatgta tttaaatcag ttttgattgc ctcacgaatg tgaatcgtat tcggtgcttt
     3301 tgataaaact tgatctgcct ctaacaataa atttttcgct gtttcaatgt cattatctaa
     3361 caccacttta cgagccgcat tattcaatag gaaatctgcc tcagataata gccagaatgt
     3421 gttatccgaa ccagaggtat tatttagctt ttgcagttga agctgcaaac tactaatttg
     3481 ctgggtataa ccattttggg ctgtttctaa agctttaatc cgttctaaag ctttttgata
     3541 ttccgtgacc aattcggcaa tttgtgcttt ttcattttcg aaatttggca tctcaaccat
     3601 agtttgagta gtaggctgat ttgctttagc agatactgcc tgaatttgag attcaacctc
     3661 tgcaaacttt ttatttgcca tataatgacc agctccgcca accgctaaag ccactaatag
     3721 tgcaactaat gccaagccct ttccgcccga tttttgaaca accacttgtt cttttggcgc
     3781 tttttgcatt tctgtttgaa tatctttctc tatagcttcg ttcactacat cttcctcaat
     3841 ctcttttttt gcaaaagatc ccgctgaatc gaccgcttgt tgtgcctctt ctaccacctt
     3901 ttcagctgaa tgctctaaat gttcagttac ttttttagat cttgacatac tcgccacctc
     3961 tagttagatt tgcccacttt aaataataat gtattcatca aagttacatt atcggcttta
     4021 tccgataccc taatcttatc ttcttgccag cccatttgtt tagctatctt tgcaatccgc
     4081 cggctcacta caaccagatc acattcaaat aaccatgctc tacaatctgc tttcgcttgt
     4141 tcataaagtg atcttaaaat ttcactactg gtgatcacaa tagtatctac tcccaagcgt
     4201 ttacataagc tgattttttc gggaatatca tcggtaaccg gctctctacg ataacactcc
     4261 aaatattgaa tagttgcccc acgcaagact gcagtttcag caagtagctc acgcccagaa
     4321 tcggctctta gaattaaaat agttttatca gttagattct gcatttcagg taatgccaaa
     4381 accccctcgc tattttcaaa ttctatcgga taaatgaccg cttgttcagc tttttctgtt
     4441 aaatatttcg cagtccgttg ccctaccgca aaatatttca aatctgaacg aaaatggaac
     4501 ccggtatcta ctaatgtttt tgcagcatag tccactgcat tcggcgaaac aacaaataca
     4561 taatcgtctg attttagccg agaaatagcc gaaggaagca aaggtaattc tttaccggct
     4621 tcaatcgtaa atagcggttg atgaatagca aaaatttgct gttcattcaa catatctacc
     4681 aattcttgcc cccgactatc caggcgtgtg accaacacat tcattattgt acgttttcgt
     4741 tataaaccgc agctaaaatt ttatctgcac cttgagcaag caactgttcc gccaccaaaa
     4801 tgcctaattg ctccgcctct tgcttatgtc cttttgccga agcccgaata atttgtgagc
     4861 catctaacga tccaactaac gcattaagcg ttaattcatc gccctctaat gtagcataac
     4921 caccaatcgg cacttggcaa ccgccttgca aacgggtatt cattgcacgt tctgccatca
     4981 cgcaacaagc ggtcgaacta tggtttaatt ttgcaatgta gttcaaaatc cgttcatcat
     5041 tggctcgagt ctcaatccct actgctcctt gaccagctgc gggtaatgat tgttccacag
     5101 aaatatagct acggattcgc tcttgcaagc ctaaacgaat taagccggca gaggctaaaa
     5161 taatcgcatc atactcgcca ttatctaatt tgcttaagcg agtgccgaca ttgcctcgca
     5221 atgatttaac ctctaaatgc ggatatttcg ccattaattg gcactgacgg cgtaagctcg
     5281 atgtaccgac cacagcacct ttcggcaaat cttctaacga atggtagttg ttcgacacaa
     5341 acgcatcacg gggatcttca cgttcacaaa tcaccgcaag ccccaagcct tcgggaaagc
     5401 tcatcggcac atctttcatt gaatgcaccg caatatcagc tcgattttct aaaagggcta
     5461 attctaactc tttcacaaac aagccttttc cgccaatttt ggctaatggg gtgtctaaaa
     5521 taatatcgcc tttcgttacc atcgtaacca gttcgacaga taattgcgga aaatgttttt
     5581 ctaattcgct ttttacaaaa tttgcctgcc ataaggctaa cgggcttt
//
LOCUS       DANLXF010000152         5628 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.152, whole genome shotgun sequence.
ACCESSION   DANLXF010000152 DANLXF010000000
VERSION     DANLXF010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5628
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.152"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            33..722
                     /locus_tag="PXD66_001842"
     CDS             33..722
                     /locus_tag="PXD66_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDL6320117.1"
                     /translation="MSTLSRDSILSQIQNGLIASCQPVDDGPMDKPEIVAAMAAASVI
                     GGAKGIRIEGVDNLKAARAAVNVPIIGIVKRDLPDSPVRITPFLEDIDALAEAGADII
                     AVDGTFRTRPVSIEEAVKRIHEKGCLAMADCSTLEEGLHCQQLGFDIVGSTMSGYTGG
                     EMPDEPDYQLVKDLKAAGCNVMAEGRYNTPELAKTAIEIGADYVTVGSALTRLEHIVS
                     WFADSVKSAQK"
     gene            732..1634
                     /locus_tag="PXD66_001843"
     CDS             732..1634
                     /locus_tag="PXD66_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HDL6320118.1"
                     /translation="MRCLAIDIGGTKIATAIVQHNQVSQRQQIQTPTDKPQADQAGSL
                     HQAIGEIMQHYQGQFDFVAVASTGIINKGVLTALNPKNLGGLAEFPLKQSIEKHTDKP
                     IALLNDVQAAVCAEYLNEDQQAVKNFVFITVSTGVGGGIILNGELQIGSNGIAGHIGH
                     TLADPNGPLCGCGRVGCVEAVAAGRAIEAVSSQWEKPCSPKEVFERFRQGNPQAVELV
                     QKSAKAIANLIADLKISLDIQKVVLGGSVGLAEGYLPQVSNYLAEMPSVYHCVLENAL
                     SGQDAGLIGAAWWAENQLNQGFTL"
     gene            1659..2537
                     /gene="nanA"
                     /locus_tag="PXD66_001844"
     CDS             1659..2537
                     /gene="nanA"
                     /locus_tag="PXD66_001844"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005718701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDL6320119.1"
                     /translation="MKNLKGIFSALLVSFNEDGTINEKGLRQIIRHNIDKMKVDGLYV
                     GGSTGENFMLSTEEKKEIFRIAKDEAKDEIALIAQVGSVNLKEAVELGKYATELGYDS
                     LSAVTPFYYKFSFPEIKNFYETIIRETGNKMIVYSIPFLTGVNIGVSQFAELFENEKI
                     IGVKFTAGDFYLLERLRKAFPNHLIYAGFDEMMLPATVLGVDGAIGSTFNVNGIRARQ
                     IFELAQQGKIKEAFEIQNVTNDLIEGILGNGLYQTIKGLLELEGVQAGYCREPMTKEQ
                     TPAQKEVTKQLKAKFL"
     gene            complement(2598..2804)
                     /locus_tag="PXD66_001845"
     CDS             complement(2598..2804)
                     /locus_tag="PXD66_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320120.1"
                     /translation="MDNKQTNGFNDIIVKADITQNIWSTGFYDIGRELKSENTRQEKV
                     RLKYNGDTLHRGRKSRMASLIASR"
     gene            complement(2762..3400)
                     /locus_tag="PXD66_001846"
     CDS             complement(2762..3400)
                     /locus_tag="PXD66_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320121.1"
                     /translation="MKKILLLVLLLTLATQADEGNNTQVCAEQQHQIIAHYFKISQEK
                     LSARTLASACKTAPNNSTETLVSYAVIEPKYSKENYQNDENTPKWTLFVARVNDNAIL
                     QSWEQDMEIDASIDVNADSLKIDTARYILNDKVRAFGVRFNNAARGPSAPEYSANDHL
                     MLFVPEKEKLKLVFDYPMTFWSILDDKETKTETQTWYCKWITNRPTASMILL"
     gene            complement(3642..4361)
                     /locus_tag="PXD66_001847"
     CDS             complement(3642..4361)
                     /locus_tag="PXD66_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL6320122.1"
                     /translation="MSKPNIYDESFSERMKWIAKNSFKDNYSEFARAVGVAQASLSRW
                     VKGEADPSRSNLVKIAEVSNVSLEWLATGKESKPQSENTSIVAREFERLEKMADDIVS
                     MINSFRTINVSAGFGSFNEGVTKPDGQEPYADSLLQSLGVKAHNCGVFWANGTSMHPT
                     IADSDQMLVDFSKREPRGDDKIYLVQNGESVWVKRIRKEWDYIELISDNESYRPIQIT
                     AEEAQNLQIIGQVVHIGHSLV"
     gene            4538..4816
                     /locus_tag="PXD66_001848"
     CDS             4538..4816
                     /locus_tag="PXD66_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL6320123.1"
                     /translation="MSVLNDVKKTAQRDWHKADILAELRKKGWSLRSLAKAGNVSHNT
                     LKSVLDKPYPKMERLVANAIGVAPEVIWAGRTAERIERNRRPTLTNKF"
     gene            4861..>5628
                     /locus_tag="PXD66_001849"
     CDS             4861..>5628
                     /locus_tag="PXD66_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL6320124.1"
                     /translation="MSELSLKTHYSVAELLSFKLSSLPSAHKNVLEKAIRENWQSQKR
                     KGRGGGVEYELISLPTEVQQEIRTKLLKLLPAEISKGELSVVRQNIDLEQITDKQLST
                     ADARIMVVRWFLMQEVQLGLSRTKTLDQVIAAVASSEIPAEICKAIMAGNSKAGGKLK
                     LSKRTLHSWVLAYEAGENSAERLKQMIPLKTQKRAVPERCGWLQAFLPFYQTFSNVAL
                     TQAYAQFAMQYEGEDLPTESQVRYALKQLPDYVVQQGR"
BASE COUNT         1678 a         1143 c         1236 g         1571 t
ORIGIN      
        1 gactaccttt tgaacataaa aaaggaactg ttatgtcaac actatctcgt gattctattt
       61 taagtcaaat tcaaaacggg ctgattgcct cctgccaacc ggttgatgac ggccccatgg
      121 ataaacctga aattgtcgcc gcaatggcag cagcctctgt gattggtggt gctaaaggca
      181 ttcgcattga aggtgtcgat aatttaaaag ccgctcgtgc ggcagtgaat gtgccgatta
      241 tcggcattgt aaaacgtgat ttgccagatt cccctgtgcg tattacgcca tttttagaag
      301 atattgatgc cttagccgaa gccggtgccg atattattgc ggtggacggc acattccgca
      361 cccgcccggt cagcattgaa gaggcggtaa aacgcattca cgaaaaaggt tgcttggcaa
      421 tggcggattg ctccaccctt gaagaaggtt tacattgcca acaattaggc tttgatattg
      481 tgggtagtac aatgtctggc tacaccggtg gcgaaatgcc tgatgagccg gactatcaat
      541 tagtgaaaga tttaaaagct gccggctgta atgttatggc ggaaggtcgc tacaacacgc
      601 ctgaactggc aaaaacggcg attgaaatcg gggcggatta cgttaccgtt ggatcggcat
      661 tgacccgctt agagcatatt gtgagctggt ttgccgactc tgtaaaatcg gctcaaaaat
      721 aggagctgct tatgcgttgt ttagcaatag acatcggtgg cacgaaaatt gccacggcaa
      781 ttgtgcaaca caatcaagtg tcacaacgcc aacaaattca gaccccgact gataaaccac
      841 aagctgatca ggcaggcagt ttgcatcaag cgattggtga gataatgcaa cattatcaag
      901 ggcaatttga ttttgtggca gttgcctcaa caggcattat caataaaggg gtgcttaccg
      961 cattaaatcc taaaaattta ggcggattgg cagaatttcc gttgaaacaa agcattgaaa
     1021 aacataccga taagccgatt gcattattaa atgacgtgca agcagcggtg tgtgccgaat
     1081 atttaaatga agaccaacaa gcggtcaaaa attttgtatt tattaccgtt tccaccggcg
     1141 taggcggcgg cattatttta aacggcgaac tgcaaatcgg cagtaacggc attgcaggac
     1201 atatcgggca tactctagcc gatccgaacg ggcctctctg tggttgtgga cgagtcggct
     1261 gtgtcgaagc agtcgcagca gggcgtgcaa tagaggcagt ttccagccaa tgggaaaagc
     1321 cttgttcacc gaaagaggtg ttcgagcgat tccgccaagg caacccacaa gcggtcgaat
     1381 tagtacaaaa atctgcaaaa gcgattgcta atttaattgc agatttaaaa atcagtttag
     1441 atatacaaaa agtggtgtta ggtggcagtg tgggcttagc ggaagggtat ttaccgcagg
     1501 tgtccaacta tttagctgaa atgccgagcg tatatcattg cgtattggaa aatgctctat
     1561 ccgggcaaga tgccggctta atcggtgcgg cttggtgggc ggaaaaccaa cttaatcaag
     1621 gttttactct ttaacaaatt ctaactccgg agatccaaat gaaaaactta aaaggtattt
     1681 ttagtgcgtt actcgtctct ttcaacgaag acggcaccat caacgaaaaa ggcttacgcc
     1741 aaattatccg ccacaacatc gacaaaatga aagtggacgg tttatatgtc ggcggttcaa
     1801 ccggtgaaaa ctttatgctt tctaccgaag agaaaaaaga aattttccgc attgccaaag
     1861 acgaagcgaa agatgaaatc gcccttatcg cccaagtcgg tagcgtaaac ttaaaagagg
     1921 cggtagaact gggtaaatat gccacagaat taggctacga cagcttatct gcggtcacac
     1981 cgttctacta caaattcagc ttcccggaaa tcaaaaattt ctacgaaacc attattcgtg
     2041 aaaccggcaa caaaatgatt gtttactcca ttccgttctt aaccggcgta aacatcggtg
     2101 tgagccaatt cgctgaatta tttgaaaacg aaaaaatcat cggtgtaaaa ttcactgccg
     2161 gcgatttcta cctgttagag cgtttacgca aagcgttccc gaaccactta atctacgcag
     2221 gtttcgatga aatgatgcta ccggcaacgg tattaggtgt ggacggtgca atcggctcaa
     2281 ccttcaacgt aaacggtatt cgtgcaagac aaatctttga attagcccaa caaggcaaaa
     2341 tcaaagaagc atttgaaatc caaaatgtaa ccaacgattt aatcgaaggc attttaggca
     2401 acggtttata ccaaaccatc aaaggcttat tggagttaga aggtgtgcaa gcaggttact
     2461 gccgtgagcc gatgacgaaa gaacaaactc cggcacaaaa agaagtgacc aaacagttaa
     2521 aagctaaatt cttgtaattt aagttgcaaa aggctaaacg aaaatcaccg cttgtagtaa
     2581 caagcggtga ttttttctca acgacttgca attaatgaag ccattctgct ttttctacca
     2641 cggtgtaacg tgtcaccatt atattttaat cgcacttttt cttggcgagt attttctgat
     2701 ttcaactctc tacctatatc ataaaaccct gtactccaga tattttgagt aatatccgct
     2761 tttacaataa tatcattgaa gccgttggtc tgtttgttat ccatttgcaa taccaagttt
     2821 gtgtttccgt tttggtttct ttatcatcta aaatagacca aaaagtcatc gggtaatcaa
     2881 aaacaagttt taacttttct ttttccggca caaataacat caaatgatca tttgcagaat
     2941 actcaggagc acttggacca cgagcagcat tattaaacct cacgccgaaa gctcttactt
     3001 tgtcgtttag aatataacgg gcggtatcaa ttttaagact atctgcattc acatcgatag
     3061 aggcatcgat ctccatatcc tgctcccacg attgcaatat cgcattatca ttcacacgag
     3121 caacaaaaag cgtccactta ggtgtgtttt cgtcgttttg ataattttct ttgctgtatt
     3181 taggctctat taccgcataa ctcactaagg tttccgttga attgttagga gctgttttac
     3241 aggcagaagc taaagtacga gcagaaagtt tctcttgaga aatcttaaag taatgagcaa
     3301 taatttgatg ttgttgctca gcacaaacct gtgtgttatt accttcatct gcttgagttg
     3361 caagcgtaag taaaagaact aagaggagta tctttttcat atagatttat ccgtatagaa
     3421 agataactca ttctatgggg tactgactct ttttgcatag agagaaagaa aaaaatttgg
     3481 tcggaaaaat aacggcgagc ctagcgaaaa caagggttcg ccgtaatttt atgggggaag
     3541 ctatgtaaaa aacaaaatga gcaggattag aaaaaaatga gcagtttttg cgatattttt
     3601 tgcataggtt tgatcggttt taaaactatt ttaaatctgt tttaaactaa agagtgtccg
     3661 atgtggacta cctgcccgat gatttggagg ttttgagctt cttcggcggt aatctgtatt
     3721 gggcggtagg actcgttatc gctaattaat tcaatatagt cccattcttt tctaatccgt
     3781 ttaacccata cgctttcccc attctgcaca agataaatct tatcatcacc acgaggctct
     3841 cgtttgctga aatctaccag catttggtca ctgtctgcaa tggtagggtg cattgatgtt
     3901 ccatttgccc aaaacacgcc gcagttgtgg gcttttacgc cgaggctttg cagcaggctg
     3961 tcggcgtaag gttcttgccc gtctggcttc gttacgccct cgttaaagct gccaaagcct
     4021 gctgaaacgt tgattgttct gaagctgtta atcattgaaa caatatcatc agccattttt
     4081 tctagtcgct caaactctct tgccacaata ctggtattct ctgattgtgg tttgctttcc
     4141 ttacctgttg ccagccattc aaggctcaca tttgacacct ctgctatttt tacaagattt
     4201 gaacgagatg gatctgcctc tccttttacc cacctagata gtgatgcttg ggctaccccc
     4261 actgctcttg caaactcact gtaattgtcc ttaaagctat tttttgcaat ccatttcatt
     4321 ctttctgaga agctctcatc gtaaatgttt ggtttactca tcttttacgt ttccattaat
     4381 aaagttttac tcgtaaaaca tactcataaa ccattgattt ttaagttatt tatgaataaa
     4441 gcaaaaatat tttagatgtt tttatgtttt acggttgatt tgtttaaagt tttacggtta
     4501 aatacacgca tcaaatagat aaaaagaggt gtatcgaatg agtgtattaa acgatgttaa
     4561 aaaaaccgca cagagagatt ggcataaagc cgatattctt gcggaacttc gtaaaaaagg
     4621 ttggtcactt cgttctttgg ctaaagcagg caatgttagc cataacacgc tgaagtctgt
     4681 tttagacaag ccttacccca aaatggaacg cttagttgca aatgccatag gggttgcccc
     4741 tgaagtgatt tgggctgggc gaacagcaga acgcattgaa cgtaatcggc gaccaacttt
     4801 aactaataag ttttaatcat aaatgatctt taacgtaaaa gatataggag aagaacattt
     4861 atgagcgaat taagtttaaa aacgcactat tcagtggctg aattgttaag ttttaagtta
     4921 tctagcctac caagtgctca taaaaatgtg cttgagaaag caataaggga aaactggcaa
     4981 tctcaaaaac gcaaaggcag aggcggcggg gttgaatatg aattaatcag tttacccacc
     5041 gaagtccaac aggaaatccg caccaagtta ctcaaacttc ttcctgctga aatcagcaaa
     5101 ggcgaattga gtgtagtgcg acaaaacatt gatttggagc agataaccga taaacaactt
     5161 tctacggcag atgctcgcat tatggtggtg cggtggttct tgatgcagga agtgcagttg
     5221 gggctaagcc gcacaaaaac gctagaccaa gtcattgcag cggtggcaag cagtgaaatt
     5281 cctgctgaaa tttgcaaagc gattatggcg ggcaatagta aagccggtgg aaagttaaag
     5341 ctatctaaac gcaccttgca tagttgggtg ctggcgtatg aggcaggcga aaattcagcg
     5401 gaacggttaa aacagatgat accgctgaaa acacaaaaac gtgccgtgcc cgagcggtgt
     5461 ggttggttgc aagcctttct gccgttttat caaaccttta gcaatgtggc actcacacag
     5521 gcttatgctc aatttgcaat gcagtatgag ggcgaagatt tacctacaga gagccaagtt
     5581 cggtatgcct tgaagcagtt gcccgattat gtggtgcaac aagggcgg
//
LOCUS       DANLXF010000153         5603 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.153, whole genome shotgun sequence.
ACCESSION   DANLXF010000153 DANLXF010000000
VERSION     DANLXF010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5603
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.153"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            134..1786
                     /locus_tag="PXD66_001850"
     CDS             134..1786
                     /locus_tag="PXD66_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDL6320125.1"
                     /translation="MTIFQVAQNWLAQDPDAETRAELEQLIQAAESDEKAKAELTARF
                     DGRLQFGTAGLRGRLQAGSMGMNRVLVAQAASGLAGFIKGYDKEPSIVIGYDGRKNSD
                     VFARDTAEIMAAAGIKTYLLPRKLPTPVLAYAIQYFDTTAGVMVTASHNPPEDNGYKV
                     YLGKANGGGQIVSPADKEIAALIDKVAAGDIRDLPRSQDFTVLDDEVVNAYIEKTASL
                     AKRPKAEINYVYTAMHGVGYEVLSKTLEKAGLPQPYLVSEQIQPDGSFPTVNFPNPEE
                     KGALDLAIKLAKEKNAEFIIANDPDADRLAVAVPDAQGNWKPLHGNVIGCFLGWYLAK
                     QFHAQGKQGVLACSLVSSPALAEIAKKYGLSSEETLTGFKYIGKVENLLFGFEEALGY
                     LVDPDKVRDKDGISAAIMFLDLVCSLKQEGKTLADYTTEFVQEFGADVSGQISIRVCD
                     LAEIGKLMTALRNNPPSEIGGFKVAEFIDHTKTTRQNDILVFVLENGSRLIARPSGTE
                     PKIKFYLDARGTDAENAEQVLAQFDESVRSLLRQEEYGKQDC"
     gene            1839..2773
                     /locus_tag="PXD66_001851"
                     /pseudo
     CDS             1839..2773
                     /locus_tag="PXD66_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250489.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
     gene            complement(2795..4876)
                     /gene="recG"
                     /locus_tag="PXD66_001852"
     CDS             complement(2795..4876)
                     /gene="recG"
                     /locus_tag="PXD66_001852"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDL6320126.1"
                     /translation="MSEQLLDGVPLTALSGVGAAISEKLSRIGINNVQDLLFHLPMRY
                     EDRTRITPIADVRPESFATIEGYVQLTEVQFGKRPILSVTLSDSTSKIMLKFFNFNAG
                     MKNSLATGVRVKAFGEIKRGRFMAEIHHPEYQIIRDNQPLVLAETLTPIYSTTEGLKQ
                     ASLRKLTEQALALLEKVKVAELLPDEYNPHKYSLKEALQLLHRPPPTISAEVLEKGDH
                     PAQKRLIFEELLAHNLAMQQLRLGVKQQYAEALTYQTDIKQRFLDSLPFKPTHAQSRV
                     TADIEQDLAKPHPMMRLVQGDVGSGKTLVAALAALLAIDNGKQVALMAPTEILAEQHA
                     NNFANWLNPFGIEVGWLAGKVKGKARTAQLEAIKNGDVQMIIGTHALFQDNVEFHHLA
                     LVIIDEQHRFGVHQRLTLREKGAKENNYPHQLIMTATPIPRTLAMTVYADLDTSIIDE
                     LPPGRTPITTVVISEDRRAEIVQRVYQVCKQEHRQAYWVCTLIDESEVLEAQAAAAIA
                     EDLQRALPDLRIGLVHGRLKPQEKQAIMAEFKAANLDLLVATTVIEVGVDVPNASLMI
                     IENSERLGLAQLHQLRGRVGRGATASHCVLMYKPPLGKISSKRLQVMRDSQDGFYIAE
                     KDLEIRGTGEILGTKQTGMAEFKVADLMRDRKMIPLVQRYAKQIISENQPLAEALIKR
                     WLDNKAEYTNA"
     gene            complement(4880..5308)
                     /locus_tag="PXD66_001853"
     CDS             complement(4880..5308)
                     /locus_tag="PXD66_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HDL6320127.1"
                     /translation="MYRYLFDTNIVSELYKLRNDKMDVNVRQWLRGVNPSQTNISCIT
                     LSEIKTGILLKARKDKEQSDRLNDWFENNVLKAYQTKAFVVNNDIALLASEYHIPNKM
                     DLNDAYIAATAKYHNLILVTRNTKDFIRCDVRLFNPFETA"
     gene            complement(5308..5595)
                     /locus_tag="PXD66_001854"
     CDS             complement(5308..5595)
                     /locus_tag="PXD66_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HDL6320128.1"
                     /translation="MAITITSREFNQNASKVLKMAEKEPVFITKWGKIVNVVSSYMDY
                     QQKKPSEPSFEELFASSENEDVSDEFWQEFEQTLVEIRKSNKPRPVDFGDE"
BASE COUNT         1471 a         1222 c         1321 g         1589 t
ORIGIN      
        1 aagtacgcta ttttttattt ctgacaaatc tttttatttg atagctcgat cacaaaaaaa
       61 gaaatatctc cttttaaata aaaggacttt gggctaatct aaacagagtt tttaaattac
      121 ataggagtaa aaaatgacta ttttccaagt tgcacaaaat tggttagccc aagacccgga
      181 tgcagagacc cgtgcggaat tagagcagtt aattcaagcg gcagaatcgg acgagaaagc
      241 aaaagcagaa ttaacagccc gttttgacgg acgtttacag tttggtacgg ctggtttgcg
      301 tggtcgctta caagcgggtt caatgggtat gaaccgtgtt ttggtggcac aagctgccag
      361 cggtttagct gggtttatta aaggttatga taaagagccg tcaattgtta ttggttatga
      421 cggtcgtaaa aattccgatg tgtttgcccg tgacaccgct gaaattatgg cagcggcagg
      481 cattaaaacg tacctgcttc ctcgcaaatt gccaacacca gtgcttgcct atgcgattca
      541 atattttgat accacagccg gtgtgatggt aactgccagc cacaacccac cggaagataa
      601 cggctataaa gtttatttag gtaaagccaa cggtggcgga caaattgttt cgccggcaga
      661 taaagaaatt gctgctctga ttgataaagt agcagcaggc gatattcgtg atttacctcg
      721 tagccaagat ttcacggtgt tagacgatga agtggtaaat gcttatattg agaaaaccgc
      781 ctcactagcc aaacggccaa aagccgaaat aaactacgtt tacactgcaa tgcacggtgt
      841 gggctatgaa gtgttaagca aaacattaga aaaagccggc ttaccgcaac cgtatttagt
      901 tagtgagcag attcagccgg acggttcgtt cccaacggtt aatttcccga acccggaaga
      961 gaaaggggcg ttagatttag cgattaaatt ggcgaaagag aaaaatgccg aatttattat
     1021 cgccaacgac ccggatgctg accgtttagc ggtagccgtg cctgatgctc aaggcaactg
     1081 gaaaccactg catggcaatg tgatcggctg tttcttaggc tggtatttag ccaaacaatt
     1141 ccacgcacaa ggtaaacaag gcgtgttggc ttgctcgtta gtatcctctc cggcgttagc
     1201 tgaaattgcg aaaaaatatg gtttaagctc ggaagaaacc ttaaccggct ttaaatatat
     1261 cggaaaagtt gaaaacctat tatttggctt tgaagaagcc ttaggttatt tggttgaccc
     1321 agacaaagtg cgtgataaag acggcatttc cgctgccatt atgttcttag atttagtgtg
     1381 cagcctcaaa caagaaggta aaacccttgc ggattacacc accgagtttg tacaagagtt
     1441 cggggctgat gtaagcggtc aaatttcgat cagagtttgt gatttagccg aaatcggcaa
     1501 attaatgacg gcgttacgca acaatcctcc aagcgaaatt ggtggcttta aagtggctga
     1561 gtttattgac cataccaaaa ccacacgcca aaatgacatt ttagtgtttg tgttggaaaa
     1621 tggcagccgt ttgattgctc gcccttcagg tactgagccg aaaattaaat tctatttaga
     1681 tgctcgtggc acagacgctg aaaacgcaga gcaagtgctg gctcagtttg acgaaagtgt
     1741 gcgttcgctg cttcgccaag aagaatacgg caaacaagat tgctaatggc attaattgag
     1801 ggggagctta ggctctccct tttttaaagg agacaataat ggctcaaaaa attattttag
     1861 attgtgatcc ggggcatgac gatgcgattg cgatgatgct cgcttggggg aatccgaata
     1921 ttgatttgtt ggcagtgact acggtggtag gtaatcaaac cttagaaaaa gtatcgcgta
     1981 atgcgttggc ggttgcggaa attgctcata tttgcaacgt tccttttgcc aaaggttgtg
     2041 ccagaccgct ggtgcgtgaa gtggaaaatg ccccggatat tcacggtgat tcgggaatgg
     2101 atgggcctgt actccctgaa tctacccgac aatttgagaa aaaacacgcc gctcaattaa
     2161 ttattgattt gataatgtcg cacccggaga aaagcattac cttagtgccg accggtgggc
     2221 aataaccaat attgctctag ctgcccgcct tgagccccgt attattgaaa gagtgaaaga
     2281 agtggtgctg atgggcggag gttatcacac cggcaactgg agtgcggtgg cagaatttaa
     2341 tattaaaatt gacccggaag ctgctcatat tgtgtttaat gccggttgga aggtgacgat
     2401 ggtcggctta gacttaactc accaagcact tgcgaccccc gatgtggttg aacgctttaa
     2461 ggcattaaac agcaaatcgg ggcagtttgt ggtggaattg ctggcgttct tcgccaaaat
     2521 gtataaacag gctcaaggct ttgatgctcc accggttcac gatgcctgtg cggtggcgta
     2581 tgtgattgac ccaagcctga ttgaagttca gcaagtgccg gtcaatattg agctaacagg
     2641 cacgcataca ctgggtatga cggtggcgga tttccgctac ccgccaaaac agtgtaatac
     2701 ttgggtagca aaaaaattag acaaatcccg cttttgggat ttggtaattg aatcggtgaa
     2761 aaacctgccg taatctaaat aacccgcttg ccggttaagc gttggtatat tccgccttgt
     2821 tgtctaacca acgcttaatt aacgcctctg caagcggttg attttcgcta ataatttgct
     2881 tggcgtaacg ttgcaccagc ggaatcattt tgcggtctcg cattaaatcc gccaccttaa
     2941 attctgccat tccggtctgt tttgtgccga gaatttcgcc tgtgcctcga atctccaaat
     3001 ctttttcggc aatgtagaaa ccgtcttggc tatcacgcat cacttgtaac cgcttgctgg
     3061 aaattttccc gagcggcggt ttgtacatta agacgcaatg cgaggcggtt gcccctcgcc
     3121 caacacgtcc acgcaactgg tggagttgag ccagcccgag tcgttcggaa ttttcgataa
     3181 tcattaggct agcattcggc acgtccactc ccacttcaat tacggtggtc gcaaccagca
     3241 aatctaaatt cgctgcctta aattccgcca taatcgcttg tttttcctgt ggtttcagcc
     3301 gtccgtgtac taacccgatg cgtaaatccg gcaaggctcg ctgtaaatct tcggcaatcg
     3361 ctgccgcggc ttgagcctcc aacacttcgc tttcatcaat cagggtgcag acccaatagg
     3421 cttggcggtg ttcttgctta cagacttggt acacccgttg gacgatttct gctcgccgat
     3481 cttcagaaat caccacagtc gtgattggcg ttctacccgg tggcagttcg tcaatgattg
     3541 aggtgtcgag gtcagcatag accgtcattg ccagcgttct cggaatgggg gtcgccgtca
     3601 taatgagctg atgcggatag ttattttctt tcgctccttt ttcccgcaag gtcagccgtt
     3661 ggtgtacgcc aaaacggtgt tgttcatcaa taatcaccaa cgctaaatgg tgaaattcca
     3721 cattgtcttg gaacagggcg tgagtgccga taatcatctg cacatcaccg tttttgattg
     3781 cctcaagttg tgcggttctc gccttgcctt tcactttacc ggcaagccag ccaacctcaa
     3841 tcccaaaggg attaagccag ttggcaaaat tattggcgtg ctgttcggca agaatttcgg
     3901 tcggtgccat taatgccacc tgcttgccgt tatcaatcgc taatagtgct gccaaggcgg
     3961 ctactaaggt tttacctgaa ccaacatcgc cttgtaccag tcgcatcatc gggtgtggtt
     4021 tggcaagatc ttgctcaata tctgcggtta ctcggctttg tgcgtgagta ggcttaaaag
     4081 gcaaactgtc gagaaaacgc tgttttatat cggtttggta agtaagtgcc tctgcatatt
     4141 gctgttttac ccctaaacgg agttgctgca tcgctaaatt gtgtgccagc agttcttcaa
     4201 aaatcagccg tttttgagca gggtggtcac ctttttccaa gacttcggca gaaatcgtcg
     4261 gcggtgggcg gtgcaataac tgcaacgctt cttttaggct gtatttgtgc ggattgtatt
     4321 catcaggcaa cagctcggcg actttcactt tctctaataa agcgagggct tgctcggtta
     4381 atttccgcag cgaggcttgt tttaacccct cagtggtgga gtaaatcggg gtcagggttt
     4441 cagccagtac aagcggttga ttatcccgaa taatttgata ttcagggtgg tgaatttccg
     4501 ccataaatcg cccacgctta atttccccaa aggcttttac ccgaacgccg gtggcaaggc
     4561 tatttttcat tccagcattg aaattgaaga atttaagcat aattttgctg gtgctgtcgg
     4621 agagtgtaac cgataaaatc gggcgtttac caaactggac ttcggtaagt tgtacatagc
     4681 cttcaatggt ggcaaagctt tccgggcgaa catcagcaat cggggtaata cgggtgcgat
     4741 cttcataccg cattggcaga tgaaacagta aatcctgcac attattaatg ccgattcgac
     4801 ttaacttttc ggaaattgcc gctcctacgc cggaaagggc ggttaatggc acgccgtcta
     4861 ataattgttc actcattatt tatgcggttt caaatgggtt aaataaacgc acatcacaac
     4921 ggataaaatc tttggtgttg cgggtgacta aaatcagatt gtggtatttg gcggttgccg
     4981 caatataagc atcatttaaa tccattttat tgggaatatg gtactctgat gccaatagag
     5041 caatgtcgtt attcaccaca aacgcttttg tttgataagc ctttaataca ttattttcaa
     5101 accaatcatt taatcggtct gattgttctt tgtctttgcg ggctttcagc aaaatacctg
     5161 ttttgatttc agataacgta atacagctga tattggtctg gctaggatta acccctctca
     5221 gccactggcg aacattgaca tccatcttgt catttcttaa cttataaagc tcggaaacta
     5281 tattggtatc aaaaaggtag cgatacatta ctcatctcca aaatcaacag gtcttggttt
     5341 attgcttttg cggatttcaa ccaatgtttg ttcaaactct tgccaaaatt catcgctcac
     5401 atcctcattt tcggaagagg cgaatagctc ttcaaagctt ggctcgctag gctttttctg
     5461 ctgataatcc atataactag acaccacatt gacgattttg ccccatttgg taataaaaac
     5521 cggctctttt tctgccattt ttaacacttt gctggcattt tgattaaatt ctctggaagt
     5581 aatggttatt gccatatcgc tct
//
LOCUS       DANLXF010000154         5564 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.154, whole genome shotgun sequence.
ACCESSION   DANLXF010000154 DANLXF010000000
VERSION     DANLXF010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5564
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.154"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            32..2254
                     /gene="glgB"
                     /locus_tag="PXD66_001855"
     CDS             32..2254
                     /gene="glgB"
                     /locus_tag="PXD66_001855"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HDL6320129.1"
                     /translation="MQDYTYSEKDAFFVERLSSGSCNDPFSYLGIHPSNNGVIIRVFL
                     PSAIACQVIDLNGKPVSSMLKIDDRGFFIAELIGKKTDFSYRLLVEYPDSTVDIEDPY
                     RFKTHIQELDNWLFAEGKQLRPYEKLGAHLVTQNEVGGVHFSVWAPNAQRVSVVGDFN
                     HWNGLVHSMRFHPDSGIWDIFLPNVKKGECYKFEVLDRNGVLRLKSDPYAFASQFRPE
                     TASIVSGLPSIVPAEKTCRNANAPDKPISIYEVHLGSWRRNIENNYWLSYEELADELI
                     PYVKDMGFTHIELLPISEFPFDGSWGYQTTGIYSPTSRFGTPEGLSYFIQKAHDKGIG
                     VILDWVVGHFPVDEHGLVYFDGTHLYEHSDPKEGYHKDWNTLIFSYGRNEVSNYLSGN
                     ALYWIERFGIDALRVDAVASMIYRDYSRSDGEWIPNKYGGRENLEAIDFLRHTNNILR
                     DESQGGASIAEESTSFAGVSHSTERGGLGFNYKWNMGWMNDTLRYMQKDPIYRRYHHN
                     LMTFGMTYQYSEQFVLPLSHDEVVHGKGSLLNKMPGDCWQKFANLRAYYGYMWGYPGK
                     KLLFMGNEFAQGKEWNFAESLDWYLLGEEGGEWHKGVLGWVKALNHLYTQTPALFEQD
                     YNPEGFEWLVVDDHDNSVFAFERKAKNGDSVIVVSNFTPIVRENYRFGVNELAEYQEI
                     LNSDLACYCGSNVSNANRFASELIASHGKAYSLSITLPPLSTIFLKRAADKKTKQNKT
                     "
     gene            2278..4290
                     /gene="glgX"
                     /locus_tag="PXD66_001856"
     CDS             2278..4290
                     /gene="glgX"
                     /locus_tag="PXD66_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HDL6320130.1"
                     /translation="MHYFRGKPFPLGNKLDRQAGEFGTNFAIVSQNATAIELCLFDEN
                     HHETRIPMFSSGDTWHIFVAGVQAGTKYGYRVKGIQDEQAGLLFNPQKLLTDPYAKAI
                     EGKPDLSSAEAAAWYKWNDERDNASLAPKSIVIDEHFDWENDSQLFTPWAETIIYEMQ
                     VKGFSQRNFQIPSDIRGTFAGLAHPVSIDYLKTLGITAVELLPVTYHIDETHLQKQGL
                     VNYWGYNVLGNFAIDPNLAADKQNPLKEFKQLVKTLHQAHIEVIMDIVFNHTAESDKN
                     GPMLSFRGIDNTAYWLDKQGEYENWTGCGNTLNLSNPYILCWAIDCLIYWVEECHIDG
                     FRFDLASILGRTPSFSEKADFFTALKSHSKLTHIKLIAEPWDIGVEGYQLGQFPEPFY
                     QWNDCYRDDIRRFWLQNQGDWGLLARRLAGSDDVFTKYSVSKSINFITAHDGFTLQDL
                     TSYQQKHNWANGEQNRDGHNSNYSNNHGVEGQTADELVKKQREITACSLLATLFLSAG
                     VPMLLAGDEIGHSQQGNNNSYCQDNEITWLDWENMNHTRLDYVRSLIRLRKKIALLSK
                     QNNWWQEDSVQWLNPSAQLMNIDDWHNQHNGLVVLLQEEWLLLFNANRQPQPFLLPQG
                     NWKVVLEQAQGILMTDTQAVTLNNMGVCVLQKSDVMFANKLETQYA"
     gene            4283..>5564
                     /gene="glgC"
                     /locus_tag="PXD66_001857"
     CDS             4283..>5564
                     /gene="glgC"
                     /locus_tag="PXD66_001857"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HDL6320131.1"
                     /translation="MLNQESTALVARQDLTNDTLVLILAGGRGSRLYELTDKRAKPAV
                     YFGGSRRIIDFALSNCINSNLLKIGVITQYAAHSLLRHLQRGWSFLPYERNQYIDMLP
                     ARQQLDENTWYRGTADAVYQNMAMMKSHYRPKYVVILAGDHIYKMDYTQMLKDHVDQG
                     AKCTVGCIEVPRSQATEFGVMAVNEKLKVKEFVEKPADPPAMPGKPDSSLASMGIYVF
                     NADYLYEMLEKEASTPGTSHDFGKDIIPKAVEQGVLYAHPFERSCKGRNASGAIYWRD
                     VGTIDSYWAAHMDLVSEDPELDLYDESWPIHGRPQQTAPARFFYNRARARTLDNSLIA
                     GGCVITDAEISNSVLFNRVHVEAESVIEHTVILPQVTIGKNCVIKRAVIDRHCVIPDG
                     LEIGVNAEEDAKRFRVSKNGIVLVTQSMLDKLAGK"
BASE COUNT         1631 a         1033 c         1297 g         1603 t
ORIGIN      
        1 aagtattaaa cgctttaagc aaggagtgcc gatgcaagac tatacttatt ctgaaaaaga
       61 tgcttttttt gttgagcgac tttcttcggg ttcttgtaat gatcctttct cctacctagg
      121 tattcaccct agcaataacg gcgttattat tagggtattt cttccttctg ccatagcttg
      181 ccaagtgatc gatttaaacg gaaagcctgt gtcatcaatg ctaaaaattg atgatagagg
      241 cttttttatt gcagagctga ttggtaaaaa aacagatttt tcttaccgct tgttggtgga
      301 ataccctgat tcaacggtgg atatagaaga cccttaccgt tttaaaaccc atattcaaga
      361 gcttgataat tggttgtttg cggaaggaaa acaacttcgc ccgtacgaaa aattaggcgc
      421 acatttagtc acacaaaatg aagttggggg tgtgcatttt agcgtctggg cgccgaacgc
      481 tcagcgggtt tccgtggttg gggatttcaa ccactggaac ggcttggtac attcaatgcg
      541 attccacccc gattccggta tttgggatat tttcctgccg aatgtgaaaa aaggggagtg
      601 ctataaattt gaagtgttgg atcgaaacgg cgtgctacgg ttgaaatccg acccttacgc
      661 ctttgcctcc caattccgcc cagaaaccgc atctattgtt tccggcttgc caagcattgt
      721 gccagccgag aaaacctgcc gtaatgcgaa tgctcccgat aaaccgattt ccatttatga
      781 agtgcattta ggctcttggc gacgcaacat tgaaaacaat tattggctta gctatgaaga
      841 gcttgccgat gaactcattc cttatgtgaa agatatgggc tttacccata ttgaattgct
      901 gcctatttcc gaatttccgt ttgatggctc ttggggttat caaaccaccg gtatttattc
      961 gccaaccagc cgttttggta cgcctgaggg cttgagttac tttatccaaa aagctcacga
     1021 taaaggcatt ggtgtgattc ttgactgggt agtggggcat tttccggtag atgaacacgg
     1081 cttggtttat tttgatggaa cgcatttata cgaacattcc gatccgaaag agggctacca
     1141 caaagattgg aataccttaa tttttagcta cggcagaaat gaggtgagca attatttgtc
     1201 tggtaatgca ctttattgga ttgagcgttt tgggattgat gcgttgcggg tggatgcggt
     1261 ggcgtcaatg atttatcgtg attactcacg ttcagacggc gagtggattc caaataaata
     1321 tggcggacgt gagaacttag aggcaatcga cttcttacgc cacaccaata atattttgag
     1381 agacgaaagc caaggcggtg cgagcattgc agaggagtcc acttcttttg ccggagtgtc
     1441 tcactcaact gaaagaggcg gtttgggctt taattataaa tggaatatgg gctggatgaa
     1501 cgacacttta cgctatatgc aaaaagatcc aatttaccgc cgttatcacc acaatttaat
     1561 gacttttggt atgacctacc aatacagcga acagtttgtg ttaccgcttt cacacgatga
     1621 agttgttcac ggcaaaggtt cattgctgaa taaaatgccg ggggattgct ggcaaaaatt
     1681 tgcgaactta cgagcctatt atggctatat gtggggctat ccgggcaaaa aattgctctt
     1741 tatgggcaac gagtttgctc aaggtaaaga gtggaatttt gccgagagct tagattggta
     1801 tttactcggg gaagaagggg gcgaatggca taagggcgta ttgggttggg taaaagccct
     1861 taaccattta tacacccaaa cccctgcact gtttgagcaa gattacaatc ctgaaggttt
     1921 tgagtggtta gtggtggacg atcacgataa ttcggtgttt gcttttgagc gtaaagccaa
     1981 aaatggcgat tcggtgattg tggtgagtaa tttcaccccg attgtgcgtg aaaattaccg
     2041 ttttggggtg aatgaattgg cagaatatca ggaaattctc aattctgatt tagcttgtta
     2101 ttgcggtagt aatgtcagca atgcaaaccg atttgccagc gagttaattg catcgcacgg
     2161 taaagcctac tccttgagca ttactttgcc accgctatcg actatcttcc tcaaaagagc
     2221 cgcagacaaa aaaacaaaac aaaacaaaac ataacttaaa gaaacataaa aaataatatg
     2281 cactatttta gaggtaagcc ttttccttta ggcaataaac ttgatagaca agcgggtgaa
     2341 tttggcacaa attttgccat tgtttcgcaa aatgcaaccg ctattgagtt atgtcttttt
     2401 gatgaaaatc accacgaaac ccgtattccg atgttttctt ccggagatac ttggcacatt
     2461 tttgtagcag gtgtacaagc agggacaaaa tatggctaca gggtgaaagg catacaagat
     2521 gaacaagccg gtttgttatt taatccgcaa aaattattga ctgatcctta cgctaaagcg
     2581 attgaaggga agccggattt aagtagtgca gaggcagccg cttggtataa atggaacgat
     2641 gaacgggata atgcctctct tgcacctaag tctatcgtga ttgatgagca ttttgattgg
     2701 gaaaatgata gccagttatt cactccttgg gcagagacta ttatttatga aatgcaggtg
     2761 aaaggtttta gtcagcggaa ctttcagatt ccaagcgaca ttcgaggcac ttttgccggt
     2821 ttggctcacc ctgtttcgat agactattta aaaaccttag gaatcaccgc agtagaactt
     2881 ctacccgtta cttaccatat tgatgaaacc catttgcaga aacaagggct tgtgaattat
     2941 tggggctata atgtattagg taattttgct attgatccga atttagcagc agataagcaa
     3001 aatcctctta aagagtttaa acagctcgta aaaacattac atcaagcaca tatcgaagtg
     3061 attatggata ttgtgtttaa ccacacagct gagagtgata aaaacgggcc gatgctaagt
     3121 tttagaggca ttgacaatac agcttattgg ctagataaac agggcgaata cgaaaattgg
     3181 actggctgtg gtaatacctt aaatttgagc aatccttata tactctgctg ggcaatcgat
     3241 tgcttaatct attgggtgga ggagtgccat attgatggat tccgctttga tcttgcctca
     3301 attctaggta gaacaccgag tttttctgaa aaagcagatt ttttcacagc acttaaaagc
     3361 cattctaaat taacacatat taagttgatt gctgagcctt gggatattgg tgtagaaggt
     3421 tatcaattag ggcaattccc tgagcctttt tatcagtgga atgattgtta tcgtgatgat
     3481 atacgccgtt tttggttaca aaaccaaggt gattgggggc ttttggcacg gcgattggca
     3541 ggaagtgatg atgtgtttac taaatattcc gtttcaaaaa gtatcaattt tattacggca
     3601 cacgatgggt ttacgttaca agatctcacc agctatcagc aaaaacataa ttgggcaaat
     3661 ggtgaacaaa accgagacgg gcataatagt aactacagca ataaccacgg tgttgaaggg
     3721 cagacagcag atgaattggt aaaaaaacag agagaaatca ccgcttgtag tttgcttgct
     3781 acgctctttt tatcggctgg tgttccaatg cttttagccg gtgatgaaat cggacatagt
     3841 caacaaggta acaataatag ttattgccaa gataatgaga tcacttggct agattgggaa
     3901 aatatgaatc acacacggct ggattatgtt cgtagtttaa tccggttgcg taaaaaaatc
     3961 gctttgcttt ccaagcagaa taattggtgg caagaggatt cggtacaatg gttgaatcct
     4021 tctgctcagc taatgaatat tgacgattgg cataatcaac ataatgggct tgtcgtttta
     4081 ttacaggaag aatggctgct tttattcaat gctaatcgac aacctcaacc gtttttactg
     4141 ccacaaggca actggaaagt tgtgttggag caggctcaag gtattttgat gacagacacg
     4201 caagcggtta cgctcaataa tatgggtgta tgcgtacttc aaaaaagtga tgtaatgttc
     4261 gctaataaat tggagacaca atatgcttaa ccaagaatca acggctcttg ttgcaagaca
     4321 agatttaacc aacgatacct tagtattaat tcttgcagga ggacgaggtt ctcgcctata
     4381 cgaattaacc gataaacgtg caaaaccggc agtctatttc ggtggtagcc gccgtattat
     4441 tgattttgcg ttatcaaatt gtattaactc gaatttatta aaaatcgggg tgattaccca
     4501 atatgcagca cactctttgt tgcgccattt acaacgtggt tggtcatttt taccttatga
     4561 acgtaaccaa tatattgata tgttaccggc acgccaacag ctagatgaaa acacttggta
     4621 tcgtggcaca gcggatgcgg tgtatcaaaa tatggcgatg atgaaatcgc actatagacc
     4681 aaaatatgtg gtgattttag caggggatca tatctataaa atggactaca ctcaaatgct
     4741 gaaagatcac gtcgatcaag gtgcaaaatg tactgtaggt tgtatcgaag tgcctcgctc
     4801 gcaagcaaca gagtttgggg taatggcggt gaatgaaaag ctgaaagtga aagagttcgt
     4861 cgaaaaacca gctgatccac ctgcaatgcc gggaaaaccg gattcctcgc tagcatcaat
     4921 ggggatttat gtatttaatg cagattatct atatgaaatg ctggaaaaag aagcctctac
     4981 accgggaacc tcacatgatt tcggtaaaga tattattcca aaagcagtag agcaaggtgt
     5041 gctatatgcc cacccatttg aacgttcttg taaagggcgt aatgccagtg gggcaattta
     5101 ctggcgagat gtcggtacga ttgacagcta ctgggctgcc catatggatt tagtttctga
     5161 agatccggaa ttagatttat atgatgaatc ttggccgatt cacggtcgtc cgcaacaaac
     5221 tgcaccggca cgttttttct ataatcgtgc gcgtgccaga accctagata actcactgat
     5281 tgcgggtggt tgtgtgatta ccgatgcgga aatcagtaat tcggtcttat ttaaccgtgt
     5341 tcacgttgaa gcggagtcgg tgattgaaca cacagtgatt ttaccgcagg tgactatcgg
     5401 taaaaattgc gtaattaagc gcgctgtgat tgacagacat tgtgtaattc ctgatggctt
     5461 agaaattggt gtaaatgccg aagaagatgc gaaacgtttt cgtgtcagca aaaatggcat
     5521 tgtattagtg acacagtcaa tgttagataa attagcggga aaag
//
LOCUS       DANLXF010000155         5533 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.155, whole genome shotgun sequence.
ACCESSION   DANLXF010000155 DANLXF010000000
VERSION     DANLXF010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5533
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.155"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..493)
                     /gene="hpt"
                     /locus_tag="PXD66_001858"
     CDS             complement(<1..493)
                     /gene="hpt"
                     /locus_tag="PXD66_001858"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDL6320132.1"
                     /translation="MEKHHIETLHSKEEVLNRINELAKEINQHYHQKQCQNLVVVGLL
                     RGSFMFMADLVRKLELPVEVDFLTASSYGSSTESSRDVKILKDLDGEIQGKDVLIVED
                     IIDTGFTLSKVGEILKLREPNSLAICTLLDKPCRREVDVPVKWVGFEIPDEFVVGYGI
                     DYAQ"
     gene            complement(532..1881)
                     /gene="pmbA"
                     /locus_tag="PXD66_001859"
     CDS             complement(532..1881)
                     /gene="pmbA"
                     /locus_tag="PXD66_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HDL6320133.1"
                     /translation="MSITDKQTLLQQEQELRQAVEFALNFAKQAGAEAEVGVTKVAGL
                     SVSSRLEQVENIEFNNDGSLGISVYLGKRKGNASTSDLQPESIQKTVEAALAIAKYTS
                     EDACTGLADKELLAFEAEDLELYHQADISVDQAVELAIEAEHYALNADEKIVNSDGAT
                     FNSHSGVRVYGNTHGMLQSYLSSRYSLSCSVISAYEDQLERDYEYTIFREFDKLQAPK
                     WVGEQAAIKAVDRLNPQRIKTGEMSVIFYNDVATGLVGHLAGAISGGALYRKSSFLLD
                     KLGEQILPEWFAISERPHLLRQLASSAFDSEGVITQNREIITDGVLQTYLMTSYSGRK
                     MGLKSTGHAGGIHNWLVKPNRQGGLNTLLKEMGTGLLVTEMLGSAINSVTGEYSRGAA
                     GFWVENGEIQYPVAEITIAGQLQDMYKNLVAVGDDIEHRSNIQTGSILLEKMMVSGE"
     gene            1980..2510
                     /locus_tag="PXD66_001860"
     CDS             1980..2510
                     /locus_tag="PXD66_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HDL6320134.1"
                     /translation="MAKKRSKNEIDWTDEEEEIIWVSKSEIKRDSEHLKKIGAELIEL
                     TPQNLEKIPLDDDLKEAIRQAQGFKMEARRRQIQFIGKLLRNRDQEPIQEALDKVKNR
                     HNQQQALLHKLELVRNQLIAMGDASLDNLLTEYPQLDRQHLRNLIRGAIKEREANKPA
                     KNYREIYQYLKTEIVE"
     gene            2516..2887
                     /locus_tag="PXD66_001861"
     CDS             2516..2887
                     /locus_tag="PXD66_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HDL6320135.1"
                     /translation="MEYLSTILKAHIGLAYISLILLLTRGFLSSKMVDWRQYKILRIA
                     PHAVDTFLLITGIAAVAIFLAYDFFTLAQFSWLLPKLLFLVLYIVFATKAFKKGQPFS
                     LKFYLLAVVSFLLMMLTATFH"
     gene            2900..3613
                     /locus_tag="PXD66_001862"
     CDS             2900..3613
                     /locus_tag="PXD66_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDL6320136.1"
                     /translation="MIGPFVNAGAIILGGVLGACLGSHLPERIRTTLPLVFGLCCFGL
                     GMMLTASVKYMSAVALALILGTIIGELIYLENRIGKVAGLARGLIDRLFPPVHSLSQE
                     EFLEKFIALLVLFCVSGTGIFGAIKEGMTGDPSILYIKSILDFFTAMIFAASLGYAIA
                     AIAVPLIIVQGILILLATVIMPLTTANMMADFSATGGLLLFATGFRICGIKNFPVANM
                     LPALLLAMPMSYFWEWLVH"
     gene            3616..4179
                     /locus_tag="PXD66_001863"
     CDS             3616..4179
                     /locus_tag="PXD66_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HDL6320137.1"
                     /translation="MKKRIIVGISGASGFQYGYKVLELLKPFSEIETHLVISKGAELT
                     RTLETDYTREALHDLADQIHSLNNVGASIASGSFKTEGMVIAPCSMRTLAAIANGLSD
                     NLLTRAADVVLKERRKLVLMVREAPLNLAHIDNMRKVTEMGGIIFPPIPAFYQKPTSL
                     DEMVTHSVSHALSLLDIDIPNTPHWGE"
     gene            4242..4682
                     /locus_tag="PXD66_001864"
     CDS             4242..4682
                     /locus_tag="PXD66_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDL6320138.1"
                     /translation="MHNPIPTHILDFIQTNHVVNIAAHTEQDFWAANCFYAFDQENVR
                     LIILTAKKTRHAGLMLENPHIVGTICGQIEKINEIEGVQFSATAECLEDKQAEKAALQ
                     IYYQKHPLARLKPSDVWVLSFNTIKHTGNKLMFAKKTIWERNII"
     gene            complement(4686..>5533)
                     /locus_tag="PXD66_001865"
     CDS             complement(4686..>5533)
                     /locus_tag="PXD66_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL6320139.1"
                     /translation="NQIKGFSHLYRLRSDLMLKYTEISVTETVEQEPAEFEAGLRLVI
                     LLSGRSEIDFNSEVFELDASLKPQAAFLPLNRAEMGRKIFRKGQLQKELVIFLYPKWL
                     IESQLNLASYHHFKQHLKAHQIILTQSMLKLIHDIVSEQEMQPFGLLEKEGNLLALLA
                     SAFSQLTQAPRLKKDRVQQLTELLESGKFDGWSLKQIAIYFHTNVTTLQNEFQHYHKT
                     SIFAYLRRSKLEQAYKLLLQGQSITQAAEFAGYANSENFSTAFKRTFGITPSQIKRDK
                     AKGII"
BASE COUNT         1590 a         1177 c         1047 g         1719 t
ORIGIN      
        1 tttgggcata atcaatccca taacccacca caaattcatc ggggatctca aatcccaccc
       61 attttacggg cacatcaact tcgcggcggc aaggtttatc taataaagta caaatagcaa
      121 gtgaatttgg ctcacgtaac ttgagaatct cccctacctt gcttaaggtg aagccagtat
      181 caataatatc ttctacaatc aatacatctt taccttgaat ttcgccatct aaatctttta
      241 gaattttgac atcacggctg gattccgtgc tgctgccata gcttgatgcg gttagaaaat
      301 ccacttctac aggtaactct agctttctta ctaaatctgc cataaacata aacgagcctc
      361 gcaataaacc aaccactaca aggttttggc actgtttttg gtgatagtgt tgattgattt
      421 cttttgctaa ttcattgatt cggtttaata cttcttcttt tgaatgtagc gtttcgatat
      481 gatgtttttc cattgatttc tccaaataaa attgattaca agcggccgtt tttactcgcc
      541 tgaaaccatc attttttcta ataagattga acctgtttgg atatttgagc ggtgttcaat
      601 atcatcgcct accgccacta aatttttata catatcttgc agttgccctg caatggtaat
      661 ttccgctaca gggtattgaa tttcaccgtt ttctacccaa aatcctgctg cacctcgaga
      721 gtattcccct gttacgctat taatcgcaga acctagcatt tccgttacta ataatcctgt
      781 tcccatttcc ttaagtaacg tatttaaacc accttgacga tttggtttaa ctaaccagtt
      841 atgaatacca cctgcgtgtc ctgtactttt taaccccatt ttacgccctg aatagctagt
      901 cattaaatac gtttgtagca cgccatcagt aataatttca cggttttggg taattacacc
      961 ttcactatca aacgcagaag aggctaattg acgcaacaaa tgcggacgct ctgaaatcgc
     1021 aaaccattca ggcagaatct gttcgcctaa tttatctaat aagaaacttg atttgcggta
     1081 taatgcaccg ccactaatag ctccggccaa atgccccact aaaccggttg ccacatcgtt
     1141 atagaaaatc acagacattt cgccggtctt aattcgttgc ggattcaatc tatctaccgc
     1201 tttaattgca gcttgctccc ctacccattt cggtgcttgt aatttatcaa actcacggaa
     1261 aatggtgtat tcataatcac gctcaagctg atcttcataa gctgaaatca cgctacaaga
     1321 gagtgaatat cggctcgaca ggtaactctg caacattccg tgcgtattgc cataaacacg
     1381 cacgccactg tgagaattga aagttgcccc gtcactattc acaatttttt cgtctgcatt
     1441 taaggcataa tgctccgctt caatcgccaa ttcgactgct tgatcaactg aaatatccgc
     1501 ttggtggtac aattccaaat cttctgcctc aaaggcaagc aattctttat cagcaagccc
     1561 ggtacaagca tcttctgagg tatattttgc aatcgcaaga gctgcttcaa ccgttttttg
     1621 aatcgactct ggctgaagat cggaggtgga agcattacct ttgcgtttgc ctaaataaac
     1681 cgaaataccg agagaaccat cgttattgaa ctcgatgttt tcaacttgct ctaaacggct
     1741 tgaaaccgaa agcccggcaa ctttagtcac gcctacttcc gcctctgccc cagcctgttt
     1801 tgcaaaattt aaggcaaatt cgaccgcttg tcgtagctcc tgttcttgtt gtaacaaggt
     1861 ttgtttatct gtaattgaca ttttgttcca ttgattgatt gcattgatga atatattcta
     1921 actcaaattt ctgttagaat acggcatttt ttagagttta tgtaatgaaa ggataaagaa
     1981 tggctaaaaa acggagcaaa aacgaaattg actggacaga tgaggaagaa gaaatcatct
     2041 gggtcagcaa aagtgagata aaacgagatt ctgaacactt aaaaaaaatt ggggctgaat
     2101 tgatcgaatt gaccccacaa aatttggaaa aaattccact agacgatgat ttaaaagagg
     2161 caattcgcca agctcaaggc tttaaaatgg aggctcgccg ccgtcaaatt caatttatcg
     2221 gcaaattatt acgcaaccgt gaccaggagc cgattcaaga agccttagat aaagtgaaaa
     2281 atcgccataa tcagcaacaa gccctattac ataaattgga attagtgcgt aatcaactga
     2341 ttgcaatggg cgatgcatca ttagataatt tattgacgga atatcctcaa ttagaccgcc
     2401 aacatttgcg taatttaatt cgtggggcaa ttaaagagcg tgaagccaat aaaccggcaa
     2461 aaaattaccg tgaaatttat caatatttaa aaactgaaat cgtggagtga ttattatgga
     2521 gtacctatca accattctta aagctcatat cggcttagct tatattagtt taattttatt
     2581 attaacccgt ggatttttat cgtcaaagat ggtggattgg cgacaatata aaattttgcg
     2641 cattgcccca cacgcagttg ataccttctt acttatcacc ggtattgctg ctgtcgctat
     2701 tttcttagct tatgattttt tcaccttagc acaattcagt tggttgctac caaagttact
     2761 cttcttagta ctttatattg tatttgcgac aaaagcgttc aaaaaaggac aaccgttttc
     2821 gctcaaattt tatctgttag ccgtagtctc attcttatta atgatgctaa ctgccacttt
     2881 tcactaaata agggaaacta tgatcgggcc ttttgtgaat gccggtgcaa ttattttagg
     2941 gggcgtgctg ggtgcttgtt tagggagtca cctgcctgaa cgtattcgca ctacgttgcc
     3001 gctggtattc ggcttatgct gtttcggctt aggtatgatg ctgactgcct cagtaaaata
     3061 tatgtctgcg gttgccttag cattgatttt aggcacgatc atcggtgaac tgatttattt
     3121 agaaaacaga atcggtaaag ttgccggttt agctcgtggt ttaattgacc gcctattccc
     3181 accggtgcat agtctttccc aagaagagtt tttagagaaa tttattgctc tgcttgtact
     3241 gttttgtgtg agcggtaccg gcatttttgg tgcaataaag gaaggaatga caggcgatcc
     3301 aagtattctt tacattaaat cgattctaga tttcttcacc gcaatgattt tcgctgcttc
     3361 gttaggctat gccattgcgg cgattgcagt gccgttgatt attgtgcaag ggattttaat
     3421 tctactggca accgtgatta tgcctttaac cactgccaat atgatggcag atttctccgc
     3481 aaccggcggt ttactgctat ttgcaaccgg ctttcgcatt tgtggaatta aaaactttcc
     3541 ggtcgcaaat atgctaccag cactactcct tgcgatgccg atgtcttatt tctgggaatg
     3601 gttggtacat taatgatgaa aaaacgtatt attgtcggca ttagtggggc aagtggtttt
     3661 caatatggct ataaagtgct tgaactgctg aaaccgtttt cagaaattga aacccacctt
     3721 gtaatcagca aaggagctga actcactcgt actctcgaaa cggattatac ccgagaagct
     3781 cttcatgatt tagctgatca aatacattcg ctaaacaatg tcggagcaag cattgccagt
     3841 ggttcattta aaaccgaagg tatggtcatc gccccgtgtt ctatgcgaac ccttgccgct
     3901 attgccaacg gtttgagcga taatttgctg acccgtgcgg cagatgtggt gttgaaagaa
     3961 cggcgtaaat tggtattaat ggtgagagaa gctccgctca accttgctca tattgacaat
     4021 atgcgaaaag tcacagaaat gggcggcatt attttcccac ccattccggc gttttaccaa
     4081 aagccaacca gccttgacga gatggtcacc cacagcgtaa gccatgccct ttccctgttg
     4141 gatattgaca ttccaaatac cccacattgg ggagaataat tcggcttatc ttttgctttt
     4201 tgaaatgcta gaatacctga taaaaactat caggtatttt tatgcacaat ccaatcccga
     4261 cccatatttt agatttcatt caaactaacc acgttgtcaa tattgcagct cataccgaac
     4321 aggatttttg ggcagcaaat tgcttttatg cctttgacca agaaaatgtt cgcttgatta
     4381 tcttaaccgc caagaaaacc agacacgctg gcttgatgct tgaaaaccca catattgtcg
     4441 gcacaatttg tgggcaaatt gagaagatca acgaaattga aggtgtccaa ttctccgcta
     4501 ccgccgaatg tttagaagat aaacaagccg aaaaagccgc tttgcaaatt tattaccaaa
     4561 aacacccgct tgcacggctt aagcctagcg atgtttgggt gttatcattc aataccatca
     4621 agcacaccgg taataaactt atgtttgcga aaaagacgat ttgggagaga aatataattt
     4681 gaagctcaaa taatgccttt agccttatcc cgtttgattt ggcttggtgt tatcccaaaa
     4741 gttcgcttaa atgccgttga aaaattctcc gaattggcat atccggcaaa ttcagctgct
     4801 tgtgtaatac tctgcccttg taatagtaat ttataggctt gttctaattt agaacgcctt
     4861 aaatacgcaa aaatagaggt tttgtggtaa tgctgaaatt cattttgtaa tgtggtcaca
     4921 ttggtatgga aatatattgc aatctgcttc aaactccagc cgtcaaattt accgctctca
     4981 agcaattctg ttaactgttg cacccgatct ttttttaacc ggggagcctg tgtcagctga
     5041 gaaaaagcac ttgctaataa agctaataga ttaccttctt tctcaagtaa cccaaatggt
     5101 tgcatttcct gctctgacac aatatcgtga ataagtttta acatactctg agtcaaaata
     5161 atttggtggg cttttaaatg ctgtttaaag tgatgatagc ttgccaaatt taactggctt
     5221 tctattagcc atttagggta gagaaagata acaagctctt tttgaagttg ccctttccta
     5281 aaaattttac gccccatttc agctctattt agcggtaaaa atgcggcttg aggttttaaa
     5341 cttgcatcta attcaaatac ttccgaatta aaatcaattt ccgatcgacc actcaacaat
     5401 ataaccaatc ttagtccggc ttcaaattcc gcaggttctt gctctacggt ttcagtaact
     5461 gaaatctctg tatatttcag cataagatct gaacgtagtc ggtataaatg tgaaaaacct
     5521 tttatttggt tag
//
LOCUS       DANLXF010000156         5519 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.156, whole genome shotgun sequence.
ACCESSION   DANLXF010000156 DANLXF010000000
VERSION     DANLXF010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5519
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.156"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            111..2177
                     /gene="glyS"
                     /locus_tag="PXD66_001866"
     CDS             111..2177
                     /gene="glyS"
                     /locus_tag="PXD66_001866"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDL6320140.1"
                     /translation="MTTQNFLAEIGTEELPPKALKKLATAFAENVENELNQAGLSFEK
                     VEWFAAPRRLAVKVLSLAESQPSKEVEKRGPAVSAAFDAEGKPTKAAEGWAKGCGITV
                     EQAERIATDKGEWLVHRAVIEGQPTKNLLVDIIAKSLANLPIPKTMRWGDKTEQFVRP
                     VHTVTLLFGAELIEGEILGVKSGRTIRGHRFLGEREFQIENADQYPQLLKDRGSVVAD
                     FNERKALILAKSQEKATALGGVADIEEDLLDEVTSLVEYPNVLTAKFEERFLAVPAEA
                     LVYTMKGDQKYFPIYSKDGKLLPHFIFVSNINPEDPSKIIEGNEKVVRPRLTDAEFFF
                     KTDLKQKLEDQLPRLETVLFQQQLGTLRDKTARIEALAGEIAKQIGADETKAKRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEDEEVAVALNEQYMPRFAGDNLPNSLVAC
                     SVALADKFDTLTGIFGIGQAPKGSADPFALRRAALGSLRIIVEKNLPLDLTDLVAKSA
                     QLFGDKLTNKDVVEDVVDFMLGRFRAWYESEGIAVDVIQAVLARRPTKPADFDARVRA
                     VSHFRTLDSAEALAAANKRVSNILAKADIAIGDIDVSACVEPAEKALAEAVIALKAEV
                     QPLIAQGDYTAVLDKLANLRQPVDAFFDGVMVNAEDQKLRQNRLAILSTLQGLFLQVA
                     DISLLQ"
     gene            complement(2282..3583)
                     /locus_tag="PXD66_001867"
     CDS             complement(2282..3583)
                     /locus_tag="PXD66_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HDL6320141.1"
                     /translation="MNIHCEKLVQSILHAVNHEVVPALGCTEPISLALASAIARQYLG
                     HLPERIEAKVSPNLMKNGMGVAVPGTATVGLPMAAAIGAIGGEPDGGLEVLKNITPEQ
                     VKQAKAMLNTDKVSVSIFETEHILYSEATLFHQANRVCVRIAEHHTNVIYIEKNGQTI
                     LSKPCAVNNDNTTEIFASLSAKDIFNFSMQIELEKIRFIEQAAQLNSALSQEGLRADY
                     GLHIGRTLQKQIGLGLISDDLLNRIVIETTSASDARMGGASLPAMSNSGSGNQGITAT
                     MPIVVVARHLNVTDEQRIRALFLSHLMAIYIHSKLPKLSALCAVTTAAMGSCAGIAWL
                     LTGKFENISMAISSMIGDISGIICDGASNSCAMKVSTSVTSSYKSILMSMDNSQVTGN
                     EGIVEHDIDRSINNLCSIASRSMQYTDRQVIEIMVNKPKLK"
     gene            complement(3597..4874)
                     /locus_tag="PXD66_001868"
     CDS             complement(3597..4874)
                     /locus_tag="PXD66_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320142.1"
                     /translation="MDTQKLSPAEFKQATKFDSTDFGWIIMSIGMAIGAGIVFLPVQV
                     GLMGMWVFLLSSIIGYPAMYLFQRLFINTLAESPECKDYPTVISGYLGKNWGIFLGVL
                     YFIMLVIWMFVYSTAITNDSASYLLTFGITNTLLSDNPFYGLVLICILVALSSRGEKL
                     LFKISTFMVLTKLFVVAALGLAMIGMWQLHNIGAMPPFGKLIKDAIITLPFTLTSILF
                     LQTLSPMVISYRAKEQSREVARYKALRAMNIAFAILFITVFFYAVSFTLAMGREEALK
                     AYEQNVSALAIAAQFFPGSWATYVGVALNIFAVMTAFFGVYLGFREATQGIVMNLLLR
                     KFPAEKINPNYVQKGIMLFSILLAWSAIVLNAPVLSFTSICSPIFGLVGCLIPAYLVY
                     KVPALFKYKGLALNIIIITGILLCISPFLTFLE"
     gene            complement(4903..>5519)
                     /locus_tag="PXD66_001869"
     CDS             complement(4903..>5519)
                     /locus_tag="PXD66_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDL6320143.1"
                     /translation="YFSLSEAIAGLLGEFCEVVIHSLNAYEKSVIKIINGHHTNRKIG
                     SPITDKGLQLLKCYQKDQSNTELVYFSRLNNGNLVKSTTHIIIGHEGKPIGLFCINLN
                     LSTPLDKTVSSFMNYDPIPPHYKSENFITHPKELIEETFLKAVTHIEQHTTKHSKLYK
                     KSIIFYLFENGIFELKEAVPIVAEKLSLTSHAIYKHLREFKNNT"
BASE COUNT         1651 a         1103 c         1248 g         1517 t
ORIGIN      
        1 ttagacatca aaattaggaa attagctaga gagctttata atgaaaatat agcacaatta
       61 gaaatagaag aaatcaaaga acatttatat aaaaattggt aatttaaaca atgacaacac
      121 aaaacttcct tgccgagatc ggcacagaag agctgccacc gaaggcactc aaaaaattag
      181 cgaccgcatt tgcggagaat gtagaaaacg aattaaacca agcaggtctt tcgtttgaga
      241 aagtggaatg gtttgctgct cctcgccgtt tggcggtgaa ggtgttgagc cttgcggaaa
      301 gccagccgag taaagaggtt gagaaacgag ggccggcggt gtcggcagcg tttgatgccg
      361 aaggcaagcc gactaaggca gcggaaggct gggcgaaggg ctgcgggatt accgtagagc
      421 aagctgagcg tattgccacc gacaaaggcg aatggttggt gcatcgtgcc gtgattgaag
      481 gtcagccgac taaaaatctg ttggtcgata ttatcgccaa atcgctggcg aacttgccga
      541 ttccaaaaac gatgcgttgg ggcgataaaa ccgagcagtt cgtccgccct gtgcataccg
      601 tgacactttt attcggggcg gagttgatcg aaggtgagat cttaggcgtg aaaagcggcc
      661 gtactattcg tggtcaccgt ttcttaggtg aacgtgagtt ccaaattgag aacgccgatc
      721 aatatccgca actgttgaaa gatcgtggat ctgtggtagc agatttcaat gagcgtaaag
      781 ccttaattct tgcaaaatca caggaaaaag caaccgcttt gggtggcgtt gccgatattg
      841 aagaagattt gctggacgaa gtgacctcgt tggtggaata cccgaacgta ctcaccgcca
      901 agtttgaaga gcgtttcctt gctgtgccgg cggaagcctt agtttacacc atgaaaggcg
      961 accaaaaata cttcccgatt tattcaaaag acggcaaatt gttaccgcac tttatttttg
     1021 tgtcgaacat caatccggaa gacccaagca aaatcatcga gggtaacgaa aaagtggttc
     1081 gcccacgttt gaccgatgcg gagttcttct tcaaaaccga cttaaaacag aagttggaag
     1141 accaactgcc acgccttgaa accgtgttgt tccagcaaca gcttggcaca ttgcgtgata
     1201 aaaccgctcg cattgaagcg ttggcaggtg aaattgcgaa gcaaatcggg gcagatgaaa
     1261 ccaaagcgaa acgtgcaggt ctcctctcaa aatgtgacct gatgaccaat atggtgtttg
     1321 aatttactga tacccaaggc gtgatgggta tgcactatgc ccgccacgac ggtgaagatg
     1381 aagaagtggc ggtggcgtta aacgagcagt atatgccacg ttttgctggc gataacttgc
     1441 caaattcatt ggtggcttgc tcggtggcgt tggcggacaa atttgacacg ctaacaggga
     1501 ttttcggcat tggtcaagcc ccgaaaggca gtgccgaccc gttcgcccta cgccgtgcgg
     1561 cacttggctc gttgcggatt attgtggaga aaaatttacc gcttgattta accgatttag
     1621 tggcgaaatc tgctcaatta tttggcgata aactcaccaa caaagacgtg gttgaggacg
     1681 tggtggactt tatgctcggc cgcttccgtg cgtggtatga gagcgaaggc attgcggtgg
     1741 acgtgatcca agccgttctc gcccgccgtc cgaccaagcc tgccgacttc gatgcccgtg
     1801 tgcgtgccgt gtctcacttc cgcacccttg acagtgccga agccttagcc gcagcgaata
     1861 aacgggtgag taacattctt gccaaagccg atattgcaat tggcgacatt gatgtttcag
     1921 cttgcgtaga gccagcagaa aaagcattag ccgaggcggt tattgcgttg aaagccgaag
     1981 ttcaaccgct gattgcacag ggcgactaca ccgctgtgct ggataaatta gcgaacctcc
     2041 gccaaccggt tgatgccttc tttgacggag taatggtaaa tgcagaagac caaaaactgc
     2101 gtcaaaaccg cctcgccatt ttaagcacgc tacaaggctt gttcctgcaa gtggcggata
     2161 tttcgctgtt gcaataacaa taagtttgca ataattgcaa aattttaatg aaaatagacc
     2221 gcttgcagta attgtgatct gcaagcggtc ttttttgctt gaaattttac caaaggcggg
     2281 attatttaag ttttggttta ttcaccataa tctcgatgac ttggcggtct gtgtattgca
     2341 tactacgaga ggcaatgctg cataagttat taattgaacg atcaatatcg tgttcgacaa
     2401 ttccttcgtt gcctgttact tggctattat ccatagacat cagaatggat ttatagcttg
     2461 atgttacgct ggtggagact ttcatcgcac aactgtttga agcaccatca cagataatac
     2521 cgctaatatc gccgatcata ctactaattg ccatactgat attctcaaat ttccctgtga
     2581 gcagccacgc tatgccggca cagcttccca tagccgcggt tgttacggca cagagtgcag
     2641 ataatttcgg taacttgctg tgaatataaa ttgccatgag gtgtgataaa aagagtgctc
     2701 taatgcgttg ctcatcagtg acatttaaat ggcgggcaac gacaacaatc ggcatagttg
     2761 ctgtaattcc ttgattacct gaacccgaat tgctcatagc aggtaaactt gcaccgccca
     2821 ttctagcatc agaggcagag gtggtttcta taacaatgcg attaagcaaa tcatcactga
     2881 ttagacctag accaatctgt ttttgcaatg tccttccgat atgtagccca taatcggcac
     2941 gtaatccttc ttgtgagagt gcggaattta attgtgctgc ttgctcaata aagcgaattt
     3001 tttccaattc gatttgcata gaaaaattaa atatatcttt tgccgaaagt gaagcaaaga
     3061 tctcagtagt attatcgtta ttgacggcac aaggtttgct taaaatagtt tgcccgtttt
     3121 tttcaatgta aatgacattg gtatggtgtt cagcaatacg aacacatact cgattcgctt
     3181 ggtgaaatag tgttgcttca gaatatagaa tatgttctgt ttcgaaaata gagacagaca
     3241 ctttatcggt atttaacata gctttagctt gcttaacctg ttctggcgta atatttttta
     3301 atacttctaa tccaccatca ggctcccctc cgattgctcc aatagctgcc gccataggca
     3361 atccgacggt tgcagttccc ggtactgcta ctcccatccc atttttcatc aagttagggg
     3421 aaacttttgc ctcaatacgt tctggtaaat gacctaaata ttggcgtgca atggcggatg
     3481 caagggcgag agaaataggt tctgtacagc ctaatgcagg gacaacttca tggttgactg
     3541 catgtaaaat ggattgtact aatttttcgc agtgaatatt catagataaa accttattac
     3601 tctaaaaatg tgaggaatgg agaaatacat aataaaatgc cggtaatgat aataatattg
     3661 agtgctagcc ctttatattt gaataatgca ggcactttat agactaaata ggccggaatc
     3721 aagcacccaa ctaagccaaa aataggacta cagattgagg taaagcttaa aaccggtgca
     3781 tttaatacaa tcgcactcca agccagcaga atggaaaata acataatccc tttctgcaca
     3841 taattaggat tgattttttc agccgggaat tttcttaata ataggttcat cacaatgcct
     3901 tgagtggctt ctctaaaacc taaatatacc ccgaaaaagg ctgtcataac ggcaaaaata
     3961 tttaatgcaa cacctacata ggttgcccaa ctaccaggga aaaattgggc tgcaattgct
     4021 aatgcggaaa cattttgctc gtatgctttg agcgcttctt ctctccccat tgcaagtgta
     4081 aaagagacag cgtaaaaaaa aacggtaata aataagatag caaatgcaat attcatcgct
     4141 cgtagggctt tatagcgcgc aacttcacga gactgttctt tagcacggta agaaattacc
     4201 attgggctga gagtttgcaa gaagagtata gaggttaggg tgaaaggaag ggtaatgatg
     4261 gcatctttta taagtttgcc gaaaggaggc attgcaccga tattatggag ttgccacatt
     4321 ccaatcattg caagccctaa ggcggcaacg acaaatagct tagttaatac cataaatgta
     4381 gatattttaa acagtagctt ttcgcctcga gaagataagg caactaatat acagattagc
     4441 actaagccat aaaatggatt atcagagagt agtgtattgg taatgccgaa ggtaagaaga
     4501 taggaggcac tgtcgtttgt aattgcggta gaataaacaa acatccaaat cactaacata
     4561 ataaaataaa gtacacctaa aaagattccc cagtttttgc ccaggtagcc actaataacg
     4621 gtggggtaat ctttgcattc cggagattct gctaaagtat taatgaacag acgctgaaaa
     4681 aggtacatcg ccgggtatcc gataatagaa gaaagtagaa aaacccacat gcccataagt
     4741 cctacttgta ccggtaaaaa gacgatgcct gccccgatag ccataccaat actcataatg
     4801 atccagccga agtcggtaga atcaaatttt gttgcttgtt taaattcggc aggcgagagt
     4861 ttttgagtat ccatagagtc tccttaaatc gtaacagtat aattaagtgt tatttttgaa
     4921 ttctcttaaa tgtttataaa ttgcgtggct ggttagggag agtttttctg ccacaatagg
     4981 tactgcctct tttagctcaa agatgccatt ttcaaacaaa tagaagataa tggatttctt
     5041 atagagtttt gaatgtttgg tagtgtgttg ctcaatgtga gtaactgctt ttagaaaggt
     5101 ttcttcaata agttcttttg ggtgagtaat aaagttttca cttttataat gaggcggaat
     5161 aggatcgtaa ttcataaaag agctaacggt tttatctaat ggtgtcgata aatttaaatt
     5221 aatacaaaat aatccgatag gtttgccctc atgcccgata ataatatgcg tggtggactt
     5281 aactaagtta ccgttattta accgtgaaaa gtaaaccagc tcagtattag attgatcttt
     5341 ttggtaacat tttagtaact gtaatccttt atctgtaata ggcgaaccta tttttcggtt
     5401 agtatgatgt ccgttaatga ttttaataac ggatttttca taggcattca atgaatgaat
     5461 aaccacctca cagaattctc ctaataaccc tgctattgct tcacttaatg agaaataag
//
LOCUS       DANLXF010000157         5510 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.157, whole genome shotgun sequence.
ACCESSION   DANLXF010000157 DANLXF010000000
VERSION     DANLXF010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5510
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.157"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            376..522
                     /locus_tag="PXD66_001870"
     CDS             376..522
                     /locus_tag="PXD66_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320144.1"
                     /translation="MLAEKPKKRWRFWYAFVNGLFFDKKHCYAAYKAEVYRKQYPPEF
                     TEIT"
     gene            629..1810
                     /locus_tag="PXD66_001871"
     CDS             629..1810
                     /locus_tag="PXD66_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HDL6320145.1"
                     /translation="MSILDTYLHGVEVVEVNAGGVTISTAATSVIGVVCTGDQADAET
                     FPLNTPVLITNPLNYLEKAGSTGTLRRTLNSIGSIVKTPTVIVRVAESDDSDTLTANI
                     VGTQENGKFTGIKALLTAQSTVFVKPKLLCVPQHDNQAVATELLSVAKKLNAFAFISD
                     NGATTKEQAYTYRQNFSQREGMMIFGDWKSYNTDKKAYDTDYAVARACALQAYIDKTV
                     GWHKNISNVELDGVTGITKAVEFDINESSTEANYLNEKGITICLNHNGFRYWGSRTLA
                     TDTRWAFQQSVRTAQIIKETIGAGLAWAVDMPLTPLRVKTILEAINNKLRSWASGDDP
                     RILGARVWVAEEITADIIKSGKFVIKYDYHWIPSLESLGLEQRVNDEYVVDLVNTLKA
                     L"
     gene            1819..2325
                     /locus_tag="PXD66_001872"
     CDS             1819..2325
                     /locus_tag="PXD66_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HDL6320146.1"
                     /translation="MGLPAKLKNFNFSVDGTSYLGETTEVTQPKLAMQLEDYRAGGMI
                     APVGVNMGLEKLELEFKIGGHEEDLLKLFGGSISGNAFRFNGAYQHDDDDSVDAVELV
                     CRGRIAEIDEGSSKAGDDTEHSYKASLTYYKKTVNGVDIIEIDTLNQIYIVDGKDRLA
                     EIRKAMGL"
     gene            2404..2718
                     /locus_tag="PXD66_001873"
     CDS             2404..2718
                     /locus_tag="PXD66_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HDL6320147.1"
                     /translation="MKKVTLKQGILRGEKRITEIEVRKPLTKQLRGTNLTSLMQLSVD
                     EWCIVLPRITTPKLDKADFATMSAADLLKLSGKALDLMSEDFDEADEEDSEEQGKGEI
                     LA"
     gene            2742..2858
                     /locus_tag="PXD66_001874"
     CDS             2742..2858
                     /locus_tag="PXD66_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HDL6320148.1"
                     /translation="MADIATVFHWTPNTFDEMTIVELGQWREKARLRNGVES"
     gene            2922..3269
                     /locus_tag="PXD66_001875"
     CDS             2922..3269
                     /locus_tag="PXD66_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320149.1"
                     /translation="MEIKTVTFSKVITAFIFHIITLFVFTQLNEQAQATFMFFWWFFI
                     SLRFILVVGIMCVGIEELKIQEYRQTKAYACFSFISNFALCLQFVYFEHLIAAILFLI
                     SYIIYHSQTVTIK"
     gene            3271..3708
                     /locus_tag="PXD66_001876"
     CDS             3271..3708
                     /locus_tag="PXD66_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6320150.1"
                     /translation="MLQQFAMMSLGYFVFMRLTIPYQDTSREMNWSHVQNDVVGAMPK
                     SQFTGKAGETMEISAELRPEITGGKMSILALEMMAEQGSAYPLISGSTFMVLGWFVID
                     RISEQETTFFADGTPRAISFSMSLKRVDDSLLANIIDEVAGFI"
     gene            3708..4946
                     /locus_tag="PXD66_001877"
     CDS             3708..4946
                     /locus_tag="PXD66_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HDL6320151.1"
                     /translation="MDFLTELTNHNHRTPAVSVTVRPKPSKDNEGEKAKDISTLITHR
                     LIQLTLTDNRGFEADQLDLELDDTDGLLALPSRGAILSVGLGWQNSPLTYKGEYTVDE
                     LTHDGPPDKVTIRARSADLRGTLTNRHERSFHRTTIGKIVKQIAEENKLKPMVGKEFE
                     NEEVKHIDQTNESSINLLQRLAEQFDAIATVKNGNLIFIKAGNATTASGKLLPLFRIT
                     RSSGDSHSFSIAEGDNYKAVKAYWHNTQTGKRGEVTWDENSQVKKVTKPTMRKKTKVK
                     RGADGKPIKGKDGKSIKETVFVKGKGRQVNAVVQSKPIESDSEAIKTLPHTYATAQSA
                     INACKRNFEKLQRGVATFSLTLAEGNAELIPELPVQVSGFKAEIDSNEWIISQVTHSL
                     NKGGGFTTALEMELKPKSED"
     gene            complement(5129..>5510)
                     /locus_tag="PXD66_001878"
     CDS             complement(5129..>5510)
                     /locus_tag="PXD66_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320152.1"
                     /translation="KADEAMHIIANNIIRKKTAVANAILALNEVDSRKSSGVQLCDLL
                     LGAILSSYQNDITSERKKELTKWIALHLGWDKLTYDTLQEEKKFNIWYFYDPTKGPRI
                     VRTRETKLKYPLLKKKEQTFQPAR"
BASE COUNT         1655 a         1158 c         1256 g         1441 t
ORIGIN      
        1 ttaagtgact aaaaaacact actgcacagg ctggcgtagc gcaccagcca atatcaacag
       61 ttgctgtcat caacatgacc gagactacgg cattcgtggc acagtaacac gagcagatgc
      121 tgataaacgc ttacgtgagt gtatgattaa aaatggcaat ccaattaggg cttggttatt
      181 ttggctagtt gtgagagctt ttggttggcg tttttataaa aataaggaga ttaagattga
      241 gaaataaaga taatactgca agcaagcaag caagcaagca agcaagcaag caagcaagca
      301 agcaagcaag caaagtgtag cgcaatttaa acaagctccg ctaccttttc cagcagccgt
      361 tgttaccgtg gtgcaatgct tgcggaaaag ccgaaaaagc gttggcgttt ttggtatgcg
      421 tttgtgaatg gcttgttttt tgataaaaag cattgctatg cagcctataa agcggaggtg
      481 tatcgaaaac agtatccacc ggagtttacc gaaatcactt aatcctgtta aaccccattt
      541 cacagcccga accgctcgca acccgagcgg ttatttttta aagtttaccc caacattcaa
      601 ccctaacctt tcccaattaa ggaatactat gtcaatttta gatacctatc tacacggtgt
      661 tgaggtggtt gaggtcaatg caggtggtgt caccatttcc accgctgcta cttcggttat
      721 cggagtagtt tgcaccggag accaagccga tgccgagact tttccgctga atacgcccgt
      781 tttaattact aacccgttaa attaccttga aaaagcaggt agcaccggca cgttacgccg
      841 cacacttaat tcgattggct caatcgttaa aacgccaacg gtgatcgtgc gagttgccga
      901 gtcggacgat agcgacacct taactgcaaa tattgtcggc acgcaggaaa acggcaaatt
      961 taccggtatt aaggcgttat taaccgctca atcaaccgtg tttgttaagc cgaaattgct
     1021 ttgtgtacca cagcacgata atcaagccgt tgcgaccgaa ctattaagcg tggctaaaaa
     1081 gctgaatgcg tttgcgttta tttcagacaa cggtgcaacc accaaagaac aggcatatac
     1141 ctatcgccag aatttctctc agcgtgaagg aatgatgatc ttcggtgact ggaaatctta
     1201 taacactgat aaaaaagcct atgataccga ttatgcagtg gcccgagctt gtgcgttgca
     1261 agcctatatc gacaaaactg tcggctggca taaaaacatc tcaaacgtag agctagacgg
     1321 tgtaaccggt atcactaaag cggtagagtt tgatattaac gagagctcaa ccgaggcaaa
     1381 ctacctgaac gaaaaaggca ttaccatttg cttaaaccat aacggtttcc gctattgggg
     1441 ttcccgcact cttgccaccg atacccgctg ggcgtttcag cagtcggtgc ggacggctca
     1501 aatcattaaa gagacaatcg gagcaggctt agcttgggcg gtggatatgc cactcacacc
     1561 gttgcgtgtg aaaacaatac tagaggcgat taacaacaag ctacgctctt gggcatcggg
     1621 tgatgaccct cgcattttag gtgctcgtgt gtgggtagct gaagagatca ccgcagatat
     1681 tatcaaatca ggcaaatttg ttattaaata cgattaccat tggattccgt ccctcgaaag
     1741 cctaggctta gagcaacggg tcaatgatga atatgtagtg gatttagtca atacacttaa
     1801 agcgttatag aggtaaaaat gggattaccg gcaaaactta agaattttaa tttttcggtg
     1861 gacggcacaa gctatttggg cgaaaccacc gaagtcacac agccgaaact ggctatgcaa
     1921 cttgaagact accgagcagg cggaatgatt gcccctgtgg gtgtcaatat gggtttagag
     1981 aaactggagc ttgagttcaa aatcggcgga cacgaagagg atttgctcaa gctatttggt
     2041 ggctcaatca gtggtaatgc gttccgtttc aacggtgcgt accagcacga cgatgacgat
     2101 tcagtcgatg cggtagagtt agtctgccgt gggcgaatcg ctgaaattga cgaaggctca
     2161 agcaaagccg gtgatgacac cgagcatagc tataaagcct cgctaaccta ttacaaaaaa
     2221 acggtgaacg gtgtcgatat tatcgaaatc gacaccctca accaaattta cattgtcgat
     2281 ggcaaagacc gcttagcgga aattcgtaag gcaatgggtt tatagatttt cccctaaatt
     2341 ttcccaaagc cccttcccct tttttcaagg ggcttttaat gataaataat taaggattaa
     2401 acaatgaaaa aagtaacatt aaaacaaggc attttgcgtg gtgaaaaacg tattaccgag
     2461 attgaggtgc gtaagccatt aaccaaacaa ctgcgtggca caaatcttac ctcattaatg
     2521 cagttaagcg ttgatgagtg gtgtattgta ctgccacgca ttaccacgcc aaaattagac
     2581 aaagccgatt ttgccacaat gtcggcagcg gatttgctaa aactctccgg caaagcctta
     2641 gatttaatga gcgaagactt cgatgaggca gacgaagagg acagcgaaga acagggaaag
     2701 ggcgagattt tagcctaatt ccacagtttg ttgatgacgc tattgctgat attgctaccg
     2761 tgtttcattg gacacccaac acctttgatg aaatgacgat tgtcgagcta gggcaatggc
     2821 gagaaaaggc ccgcttacga aatggggtgg aaagttaaaa caagcggtcg tttttcgcaa
     2881 aaaatttgca aaactcgacc gcttgctata aggattttaa aatggaaatt aaaacagtta
     2941 cattcagtaa agtgattaca gcattcattt ttcatatcat tacgttattt gtttttactc
     3001 aacttaatga gcaagctcaa gctaccttta tgttcttttg gtggttcttc atttctctgc
     3061 gattcatcct tgtagttgga ataatgtgtg ttggtattga agaacttaaa attcaggaat
     3121 accgacaaac aaaagcttat gcttgtttta gtttcatatc caattttgcg ttatgcttgc
     3181 agtttgttta ctttgagcat cttattgcgg caattttatt tttgatttct tacatcatat
     3241 atcatagcca aactgtaacg attaaataat atgctccaac aattcgcaat gatgtcgcta
     3301 ggctattttg tgtttatgcg attgacaatc ccttatcaag acaccagccg agaaatgaac
     3361 tggtcgcacg ttcaaaatga tgtggttggg gcaatgccaa aaagccaatt taccggcaag
     3421 gcaggggaga caatggagat ttcagccgag ctacgccctg aaattaccgg gggcaaaatg
     3481 tcaatccttg cccttgagat gatggcagag caaggctcgg cttatccgtt gattagtggt
     3541 tcaaccttta tggtgctagg ctggtttgtg attgatcgaa tcagcgagca ggaaaccacc
     3601 ttttttgcag acggcacgcc ccgggcaatt agcttttcga tgtcgcttaa acgggttgat
     3661 gatagtctgc tcgccaatat tattgatgag gtcgcagggt ttatttaatg gattttttaa
     3721 cagagctaac caatcataac caccgcacac cggctgtttc ggtgacggtt cgcccaaagc
     3781 caagcaaaga taatgagggc gaaaaagcca aagatatttc caccctgatc acgcaccgct
     3841 taattcaact caccttaacc gacaaccgag gctttgaggc agaccagctg gatttagagc
     3901 tggacgacac tgatggctta ctcgctttgc caagtcgagg agcgatttta tcggtcgggt
     3961 taggctggca aaacagcccc ctgacctaca agggcgaata cacggtggac gagctaaccc
     4021 acgatggccc acctgataaa gtcacgattc gggcaagaag tgctgatttg cgaggcacgc
     4081 tgaccaatcg ccacgagcgg agctttcacc gcaccaccat tggtaaaatt gtgaaacaga
     4141 tcgccgaaga gaacaaactc aagccgatgg tgggcaagga gtttgaaaac gaagaggtga
     4201 aacacattga ccaaacaaac gaaagctcta ttaatctctt gcaacgcctc gcagaacaat
     4261 ttgatgccat tgctaccgtg aaaaacggca atttgatttt cattaaagcc ggaaacgcca
     4321 caaccgcaag cggtaaactg ttacccctgt ttcgcattac ccgcagttcg ggggattccc
     4381 atagcttttc gattgccgaa ggggataact acaaagcggt taaagcctat tggcacaaca
     4441 cgcaaaccgg caagcgaggc gaggtgacgt gggacgaaaa cagccaagtt aaaaaagtga
     4501 ctaagcccac aatgcggaaa aagactaaag taaaacgtgg ggcagacggc aagccgataa
     4561 aaggcaaaga cggcaagagc attaaagaga ccgtctttgt aaaaggcaaa ggcaggcaag
     4621 ttaatgcagt ggtgcaaagt aaaccgattg aaagtgatag cgaggcaatt aaaaccctac
     4681 cccatactta cgctaccgca caatcagcga ttaatgcttg taaacggaat tttgaaaaac
     4741 tacaacgagg tgtggcaact tttagcctaa ctcttgccga gggcaatgcg gagcttatcc
     4801 ccgaattacc ggttcaggtt tcaggcttta aggcggagat tgatagcaac gagtggataa
     4861 tttcgcaggt tacgcacagt ttaaataaag gtggtgggtt tacgacagcg ttagagatgg
     4921 agttgaagcc gaagtctgaa gactaacgct atttttataa aatcactata aaaaagcctt
     4981 gcatctctga tcttgaacaa gtaatattaa tcaaagttac agatttattt acataaaaca
     5041 caaagaatca taggggcggc tactttgtgt gattccgccc agtgaggaaa ttgggcgttg
     5101 ttttacacat ataagtcctt gaggacactc accgtgcagg ctggaaggtc tgctctttct
     5161 tttttaatag tgggtattta agttttgttt ctctcgttct tactattcta ggtccttttg
     5221 tagggtcgta aaaataccaa atattaaatt tcttttcctc ctgtaaagta tcataagtta
     5281 atttatccca acctaggtgc aaagcaatcc attttgttag ttctttttta cgttccgatg
     5341 taatatcatt ttgatagcta cttaatattg ctcctaataa taaatcgcat agttgaacgc
     5401 cagatgattt acgagaatct acttcgttta atgctagaat agcatttgct acggcagtct
     5461 ttttacgaat tatattatta gcgataatgt gcattgcctc atcagcttta
//
LOCUS       DANLXF010000158         5504 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.158, whole genome shotgun sequence.
ACCESSION   DANLXF010000158 DANLXF010000000
VERSION     DANLXF010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5504
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.158"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..2569
                     /gene="topA"
                     /locus_tag="PXD66_001879"
     CDS             <1..2569
                     /gene="topA"
                     /locus_tag="PXD66_001879"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDL6320153.1"
                     /translation="AKKINQYLGSNYVVKSSVGHIRDLPKSGSDEKTEKAKPISTKGL
                     TAAEKAKIKAEKERVALVKRMGIDPYNGWKANYQILPDKEKVVSELKSLAKKADHIYL
                     ATDLDREGEAIAWHLREIIGGDDSRFSRVVFNEITKDAIKKAFDDPQPLNIDRVNSQQ
                     TRRFLDRVVGFMVSPLLWKKVARGLSAGRVQSVAVKLLVEREREIKAFQSEEFWTILA
                     NTQINRKKLPLELTTYKGKKFSASNEAEANVAVRALEQSDFIVSNIEAKETSSRAAAP
                     FITSSLQRAASTRLGFGVKKTMMLAQRLYEAGYITYMRTDSTNLSREALAMARGYIQD
                     NFGANYLPEKANVYRSKDNAQEAHEAIRPSDVNVKMADLKGMDKDAERLYDLIWRRFL
                     ACQMLPARYDSTAVTVSAGDYELKANGRVLRFDGWTKVLPVQGKNPEDQELPAVELHQ
                     KLTLDEIVPSQHFTKPPARFNEASLVAELEERKIGRPSTYASIISTIQERGYVRTENK
                     RFYVEKIGEVVTDRLNESFKDLMNYDFTANMEEVLDQIAEGSKNWKEELNTFFKHFSE
                     ELSQAELDELEGGMKPNSLVPTDIDCPTCGRKMAIRTASTGVFLGCTGYALPPKERCK
                     TTMNLIPEAEFFNVLDSDSETKALMARKRCPKCDSAMDSYVIDAERKLHVCGNNPNCD
                     GHIIEQGSFKIKGYDGPVVECDKCGSDMHLKLGRFGKYMGCTSCDNTRKILKNGEVAP
                     PKEDPIAFPELKCEKADAYFVLRDGASGVFMSAHNFPKVRESRAPKVVELAQYRERLP
                     EKLQYLADAPQQDPEGNEAIIRFSRKEKRQYVTSEKNGKATKWIVDYLDGKWVERKK"
     gene            complement(2619..3557)
                     /gene="yvcK"
                     /locus_tag="PXD66_001880"
     CDS             complement(2619..3557)
                     /gene="yvcK"
                     /locus_tag="PXD66_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HDL6320154.1"
                     /translation="MENTDIIPFKPTRHHYLNQLNAVVALGGGHGLGRLLSALSFLKE
                     RLTGIVATTDNGGSTGRIRSQQGVIAWGDLRNCLNQIIVKPTIASRLFEYRFAGTGEL
                     AGHNLGNLILTALENMQIRPLEGINLVRELLHVRSQLIPMSEIPVHLIAQLHSGESVF
                     GEVQIDSLAETPQHLALDTMVVATPEAISAIQSAELILLGPGSFFTSIMPNLLVAGIA
                     NSIAQSKARVVFIDNIGQEHGPAGQLSLNQRIEWMENCIGQARINAVITTDDAGSELP
                     EHIQILRKNLSANDVYYRHDRNKLSKAIDELLAQNI"
     gene            complement(3571..4647)
                     /locus_tag="PXD66_001881"
     CDS             complement(3571..4647)
                     /locus_tag="PXD66_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL6320155.1"
                     /translation="MFQMLQDWYRKKFTDPQIVVLFSILLIGFGIIYFFSDLLMPLLV
                     ALVFAYLLEWPIRFLSSKLKLPRTLSVILVLGGFIALLSFLGVVLLPSLWNQAVTFIQ
                     DLPSMFNLLNAWLQALPEHYPELVDYATLDSIVNTAKSNILSMGESLLTLSINSIISL
                     VGLGIYTFLVPLMVFFLLKDKIVLMRSFSKVLPQNRRLATRVWFEMQQQIANYIRGKF
                     LEILIVGVVTYIIFLFFDLRYPLLLSVAVGISVLVPYIGAVLVSIPVMLIALFQFGLS
                     PDFYYLMLAFIISQLLDGNLLVPFLFSEAVNLHPLTIIIAVLIFGGLWGFWGVFFAIP
                     LATLVKAVLQALPSAQSDAIILEK"
     gene            complement(4678..5433)
                     /locus_tag="PXD66_001882"
     CDS             complement(4678..5433)
                     /locus_tag="PXD66_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL6320156.1"
                     /translation="MKKEEVGHHFLARLGKTRLRPGGKLATDWLIANGDFDKNKKVLE
                     VACNMGTTAIQLAQQFECQIVGIDLDEDALEKARKNIKENHVEHLIQVQRANATKLPF
                     DDESFDIVINEAMLTMLPLEAKEKAIREYFRVLKPNGFLLTHDIVIQDKDSDTLLAEL
                     RDAINITVSPLAKADWKSLFQRCGFRNVDTFSGEMSLLSPKGLIHDEGVLGAMKIIGN
                     ALKPENRETFNKMYKLFNDPDKKLGFIAVCSQK"
BASE COUNT         1730 a         1041 c         1199 g         1534 t
ORIGIN      
        1 ggcaaaaaaa attaaccaat atcttggtag taattatgtg gtgaaatcga gtgtggggca
       61 tattcgtgat ttaccgaaaa gtggtagtga tgaaaaaacg gaaaaagcga aaccgatttc
      121 aaccaaaggg ctgaccgctg ccgaaaaagc taaaatcaaa gcagaaaaag agcgtgttgc
      181 attggttaaa cgtatgggta tcgaccccta taatggctgg aaagctaact atcagatttt
      241 gccggataaa gagaaagtgg tttccgagct aaaatcgttg gcaaagaaag cagatcatat
      301 ctatctggca accgacttgg atagagaagg ggaggcgatt gcgtggcatt tgcgtgaaat
      361 cattggtggt gatgacagcc gttttagccg agtggtattt aatgaaatca ccaaagatgc
      421 gatcaaaaag gcttttgacg atccgcaacc gctcaatatt gaccgtgtaa attcccagca
      481 aacccgccgc tttttagacc gtgtagtggg ctttatggtg tcgccattat tatggaaaaa
      541 agtagctcgt ggtttatcgg cagggcgtgt gcaatcggtt gccgtaaaat tactggttga
      601 gcgtgagcgt gaaatcaagg cgttccaaag cgaggagttt tggactattc ttgccaatac
      661 gcaaattaat cgtaaaaaat taccgcttga attaactact tataaaggta aaaaattctc
      721 ggcaagcaat gaggctgaag caaatgttgc agtgcgtgct ttggagcaga gcgattttat
      781 tgtttctaat attgaagcca aagaaaccag ctctcgagct gctgcaccat ttattacctc
      841 atcgttacaa cgtgccgcta gcactcgttt aggttttggg gttaaaaaga cgatgatgtt
      901 agcccaacgg ttatatgaag cgggttacat cacctatatg cgtactgatt caaccaacct
      961 tagccgtgaa gcattagcaa tggctcgagg ttatattcag gataatttcg gggcgaatta
     1021 tttgccggaa aaagcaaatg tgtatcgcag taaagataat gcccaagaag ctcacgaagc
     1081 cattcgcccg tcggatgtga atgtcaaaat ggcggattta aaaggaatgg ataaagatgc
     1141 cgagcgttta tacgatttaa tttggcgtcg tttcttggcg tgtcaaatgt tgcctgctcg
     1201 ttatgattct accgcagtca cagtgtctgc cggagactat gaactcaaag caaacgggcg
     1261 tgtactgcgt tttgacggct ggacgaaagt actgccggtg caaggcaaaa atccggaaga
     1321 tcaggaatta ccggcggttg aactgcatca aaaattaacg cttgatgaaa ttgtgccaag
     1381 tcagcatttt actaaaccgc ctgcacgttt taatgaggcg tctttggttg ctgagttaga
     1441 agagcgaaaa attggccgcc cttcaaccta tgcttcaatt atttcgacga ttcaagaacg
     1501 tggctatgtt cgcaccgaaa acaagcgttt ttatgtggaa aaaatcggtg aagtagtgac
     1561 ggatcgcctc aacgaatcat tcaaagattt aatgaattat gatttcactg ccaatatgga
     1621 agaggtgctc gaccaaattg cagaggggtc taaaaattgg aaagaagagt tgaatacttt
     1681 ctttaaacat ttctctgaag agttgagcca agcagaatta gatgaacttg aaggcggaat
     1741 gaaaccaaac agccttgtgc caaccgatat tgattgcccg acttgtggac gaaaaatggc
     1801 aattcgcacc gcttccacag gggtattttt aggttgcacc ggctatgctt taccgccaaa
     1861 agagcgttgc aaaacgacaa tgaatttaat tccagaggcg gaatttttca atgtgttaga
     1921 ttcggattct gaaaccaaag ccctaatggc tcgcaagcgt tgcccgaaat gcgattcggc
     1981 aatggatagt tatgtgattg atgctgaacg taaattacac gtttgtggta ataatccaaa
     2041 ttgtgatggt catattattg agcaaggttc cttcaaaatt aaaggctatg acggacctgt
     2101 agtggaatgc gataaatgcg gttcagatat gcacttaaaa ctgggtcgtt tcggcaaata
     2161 tatgggctgc acaagctgcg ataacacccg taaaattctg aaaaacgggg aagttgctcc
     2221 gccaaaagaa gatcctattg cttttcctga attaaaatgt gaaaaagccg atgcctattt
     2281 tgtattaaga gacggagcaa gtggggtgtt tatgtcagct cataacttcc cgaaagtgcg
     2341 tgaaagccgt gcaccaaaag tggtagagtt agctcaatat cgtgaacgtt tgccggagaa
     2401 attgcagtat cttgccgatg ctccgcagca agatccggaa ggaaacgagg caattatccg
     2461 ttttagtcgt aaagagaaac gccaatatgt gacttctgag aaaaacggca aagccaccaa
     2521 atggattgtt gattatctag acggtaaatg ggttgagcgt aagaaataaa gcttaaaaga
     2581 caagcggtaa aattttctaa aaattttacc gcttgctttt agatgttctg agcgagtagt
     2641 tcatcaattg ctttacttaa tttattgcga tcgtgtcggt aataaacgtc attcgcagat
     2701 aagttttttc gcaagatttg aatatgttcg ggaagttcag aaccggcatc atcagtcgta
     2761 attaccgcat taattctagc ctgtccgata cagttctcca tccattcaat tcgctgatta
     2821 agtgatagtt gcccagctgg gccatgttct tgtccgatat tgtcaataaa tactactcgg
     2881 gctttgcttt gagcaataga gtttgctatg cctgccacca gcaaattcgg cataatactg
     2941 gtaaaaaagc tacctgggcc tagcagtatt aactcagcac tttgaatagc tgaaatggct
     3001 tctggtgtag caacaaccat agtatctaac gctaaatgtt gtggtgtttc tgctaaagaa
     3061 tcaatttgca cttcaccaaa tactgattcg cctgaatgca actgtgcaat taaatgcacc
     3121 ggtatttcag acattgggat aagttgcgat cttacgtgca ataattctcg caccaaatta
     3181 atcccttcta gcggtcggat ttgcatattt tcaagggcgg tgagaattaa attgcctaga
     3241 ttatgtccag cgagttcgcc tgtgccagca aaacgatact caaacaaccg agaagctatc
     3301 gttggtttaa cgataatttg atttaaacag ttgcgtaaat ctccccaagc aatcacccct
     3361 tgttgtgaac gtattctacc ggttgagcct ccattatcgg tagtggcaac aatgccggta
     3421 agccgttcct ttaaaaaaga gagcgcggag agcaatcgac ctaagccgtg gcccccacct
     3481 agtgcaacaa ctgcattgag ttggtttaaa tagtgatgtc gggtaggttt aaaaggaata
     3541 atatctgtat tttccatctt gggatttcgt ttatttttct aaaataatgg catctgactg
     3601 ggctgaaggc aatgcttgta atacagcttt aaccaaagtc gctaaaggaa tcgcaaaaaa
     3661 cactccccaa aagccccata aaccaccaaa gatgagtacg gcaataataa ttgtcagagg
     3721 gtgtagattt actgcttcag aaaataaaaa gggtacgagt aaatttccgt ctagcaactg
     3781 actaataata aatgcgagca ttaaatagta gaaatccggc gataacccga actggaatag
     3841 agcaattaac attaccggaa tgctgactaa aaccgcaccg atgtagggaa ctaacactga
     3901 aatccctacc gctacagaaa gtagtaacgg atagcgtaaa tcaaaaaaga ggaaaataat
     3961 ataagtgaca accccaacaa ttagaatttc taaaaattta cctcgaatat agttggcaat
     4021 ttgttgttgc atttcaaacc atactcgtgt tgcaagtcga cggttttgcg gtaatacttt
     4081 actaaaagaa cgcattaaaa cgattttatc tttcagtaag aaaaagacca tgagtgggac
     4141 taagaaggtg taaatgccta agccaactaa gctgatgata gagttaatcg agagtgttag
     4201 aagtgattct cccatactca aaatattact tttagcggta ttcacaatgg aatctagggt
     4261 cgcataatct actaattcag ggtaatgctc cggtagggct tgtaaccaag cattgagcag
     4321 attaaacatc gaaggtaaat cttgaataaa ggtaactgct tgattccaaa ggctgggtag
     4381 tagtactacg cctaaaaaag ataataaagc gatgaaacct cctagtacta gaatgacact
     4441 caaagttcga ggtaatttga gtttgctcga caaaaaacgg attggccatt ccaataaata
     4501 ggcaaacaca agtgcaacca gaaggggcat aagcagatcg ctaaaaaaat agataatacc
     4561 gaaaccgatc aatagaatac taaacagcac aactatttgc gggtcggtaa atttttttcg
     4621 ataccaatct tgcaacattt gaaacatatg gattgccttt aaattatgcg atgaaaatta
     4681 tttctggcta cagactgcaa tgaaccctag ctttttgtcc gggtcattaa atagtttgta
     4741 cattttatta aaggtttcgc gattttctgg ttttaatgca ttgccgataa ttttcatggc
     4801 gcctaaaaca ccttcatcat gaatcagacc tttaggggaa agtagagaca tttctccgga
     4861 aaaggtatca acattacgga aaccacaacg ttggaataat gatttccagt ctgctttagc
     4921 aagtggagag acagtgatat tgattgcatc acgcaactcg gctaataaag tatcggagtc
     4981 tttatcttgg ataacaatat cgtgcgttaa taaaaagcca tttggtttta atacacgaaa
     5041 atattcacgt attgcctttt ctttcgcttc tagaggaagc attgtcagca tggcttcatt
     5101 aattacaata tcaaaacttt cgtcgtcaaa tggcagtttt gttgcgttgg cgcgctgtac
     5161 ttgaattaaa tgctcaacat ggttttcttt gatattttta cgtgcttttt caagcgcatc
     5221 ttcgtctaaa tcaataccaa caatttggca ttcaaattgt tgagctaatt ggattgcagt
     5281 agtccccatg ttacaggcaa cttcgagcac ttttttattt ttatcaaaat ccccgtttgc
     5341 aattaaccaa tcagttgcta atttcccccc ggggcgtaaa cgagttttgc ctaaacgagc
     5401 taaaaaatga tgaccaactt cttctttttt cataaaaacc tctggatttg caaaatacgt
     5461 taaatatatg accgcttgta ttgtgatcta tgttcgttat tata
//
LOCUS       DANLXF010000159         5477 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.159, whole genome shotgun sequence.
ACCESSION   DANLXF010000159 DANLXF010000000
VERSION     DANLXF010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5477
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.159"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(417..1010)
                     /locus_tag="PXD66_001883"
     CDS             complement(417..1010)
                     /locus_tag="PXD66_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320157.1"
                     /translation="MHNNLAIFYCGTNGAGKSTLRSFNQDKVEIVIDSDNIAMQINPL
                     NPRLADIEAGRKAIELFQFAVKNQVAFSMESTLSGKSILKRMENAKAGYFKTRLNYIG
                     VDSYKINIARVKARVKAGGHFIDEETIRKRYHISRENLIEAIFINDETLIYDNSETKP
                     NLILQIADKKVIKISDKIPNWCNQLCDELVELGFKTT"
     gene            complement(1000..1170)
                     /locus_tag="PXD66_001884"
     CDS             complement(1000..1170)
                     /locus_tag="PXD66_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320158.1"
                     /translation="MTSREELLKKQRELDILFTAWFEEKKKHEVLTYRRENGDLIQHY
                     PDGTEKVIEYAQ"
     gene            complement(1172..1914)
                     /locus_tag="PXD66_001885"
                     /pseudo
     CDS             complement(1172..1914)
                     /locus_tag="PXD66_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
     gene            complement(1911..2354)
                     /locus_tag="PXD66_001886"
     CDS             complement(1911..2354)
                     /locus_tag="PXD66_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL6320159.1"
                     /translation="MLKNLSWRAKRFDELSTQELFEIYHARTAVFVVEQNCPYQEVDN
                     KDLQAVHFFTKNAKNLTAYCRLIPANDGVHIGRVLVVKENRGSGLARELVQKAMDYCG
                     EHFPAQPIHAQAQSYLQGFYESFGFKAVSEVYLEDGIAHLDMVLE"
     gene            complement(2348..2785)
                     /locus_tag="PXD66_001887"
     CDS             complement(2348..2785)
                     /locus_tag="PXD66_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HDL6320160.1"
                     /translation="MKQVQFYLLSQADAAELSAAEMQACELAAQAWRLGKRVLIACET
                     EQQALNIDEALWARDPDSFVPHNLSGEITTYPTPIEISWKGKRNAQRRDLLISLQTQL
                     PEFIGSFTQIIDFVPVEETAKAQARDRYKQYRQLGWQLETIQC"
     gene            3024..3467
                     /gene="lysM"
                     /locus_tag="PXD66_001888"
     CDS             3024..3467
                     /gene="lysM"
                     /locus_tag="PXD66_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HDL6320161.1"
                     /translation="MGLFDFVGDIGRKLFNKEEDASKAVTEHLAEDNPGVENVNVTVE
                     NGVAKISGIASTAAAVEKAVLMAGNVAGITEVNIDEVTLPDDVQLASDDELYVIQKGD
                     TLWEIATKAYGNGAKYKAIVEANKEVIKDENKIFPGQKIRIPKGL"
     gene            complement(3521..4372)
                     /locus_tag="PXD66_001889"
     CDS             complement(3521..4372)
                     /locus_tag="PXD66_001889"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005701564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDL6320162.1"
                     /translation="MKQYLDLCNRIVSEGKWVANERTGKHCLTVINADLTYDVEKGEF
                     PLVTTRRSYWKAAIAELLGYIRGYSNAADFRQLGTKSWDANANENAQWLANPYRKGED
                     DMGLVYGAVGRNFPKPDGGSVDLLRQIVDDLKKGIDNRGEIYTFYHPGAFHMGCLRPC
                     LHSHHFSLLDGTLYLNSTQRSADVPLGLNWNMIQCYTFLALMAQITGHKAGQAFHKIV
                     NAHIYEDQLELMRDVQLKREPLNAPKLIINPDIKSLEDLETWVSLDDFKVEGYEYHPA
                     IQYPFSV"
     gene            4465..5016
                     /locus_tag="PXD66_001890"
     CDS             4465..5016
                     /locus_tag="PXD66_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320163.1"
                     /translation="MKGIMKSILVFSTAVFLAACSSNKSGWTRLNDNDVDQKSYAIAY
                     GATAQTYADRVNESYDINSFMNGVNDWYTKKVTLPIEQIRAITINRMLDHNVYAYYSG
                     VIYAADLQANFNHLDPNCWGLVQPASMTQGIHDAMLDLQKNKVRGDEYIKNGVEQILH
                     LCVKTIAEDENESQSKGKTKKHK"
     gene            5225..5319
                     /locus_tag="PXD66_001891"
     tRNA            5225..5319
                     /locus_tag="PXD66_001891"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5260..5262,aa:Ser,seq:gct)
     gene            5333..5409
                     /locus_tag="PXD66_001892"
     tRNA            5333..5409
                     /locus_tag="PXD66_001892"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5367..5369,aa:Arg,seq:acg)
BASE COUNT         1608 a         1041 c         1074 g         1754 t
ORIGIN      
        1 tccaaataat aggcatgttt gcgatgcgtt ctaaaatatc agttgtgaag atactccata
       61 tggcagatat aagaataata ggaattttgt tattttttat gaactgtttt aagccattct
      121 cttttttatg agtaccaata acttttttag actttttcct attctgtttc atctttgtgt
      181 ttttgttcca atatccccga tggtgatcgt cttcgacgag agcttattta tttaagaacc
      241 tatcagagat aggcataagc ggtctgtttc gagaagtttt ttgcaaagtt tttctttaat
      301 ccgacagctt gtcacccaca cacaagccgt gtggggttac gcatagctga gccactttgt
      361 ggctctaagt tacagctcca aaggagctgg gattgtcgag cctagcacgg ctcgtgctag
      421 gtggttttaa accctaattc cactaattca tcacaaagct gattacacca attcggtatt
      481 ttgtctgaaa tttttatcac tttcttatca gcaatctgca aaatcaaatt cggcttggtt
      541 tctgaattat cataaatcag tgtttcatca ttgataaaaa tcgcttcaat taaattttca
      601 cggctaatgt gatagcgttt tctaatggtt tcttcatcaa taaaatgtcc gccggctttc
      661 actcttgctt ttacccgtgc gatgttaatt ttatagctat cgacaccaat ataatttaat
      721 cgagttttga aatatcctgc tttggcattt tccattcgtt ttagaataga tttacccgag
      781 agggttgatt ccattgaaaa tgcaacctga ttttttactg caaactgaaa tagttcgatt
      841 gcttttctgc ccgcctcaat atcggccaat cttggattga gtgggttaat ctgcatcgca
      901 atattatcgg aatcaatcac aatctcgact ttatcttgat tgaaagaacg taaagtagat
      961 ttgccggctc cgtttgtgcc acagtaaaaa attgctaggt tattgtgcat attcaatcac
     1021 tttttccgta ccgtccggat aatgctgaat taaatcccca ttttctcggc gatacgtcag
     1081 cacttcgtgc tttttctttt cttcaaacca tgctgtaaag agaatatcca gttcacgttg
     1141 ttttttcagt aattcttcac gacttgtcat tttatgtcct ctgtttcaga atcgccaaat
     1201 acctttccaa ctcagctaat tcatactgac taatcaattc aaccaaacta ctaatatctt
     1261 gttgattttt atggaaatca tcaaaacatt ggtaatattg ggctcgattg gtaaatttaa
     1321 tatcaatcgg taaatagccc tgttttaata attcaaaatt aatcagtaat cgccctgtga
     1381 gcccgtttcc atcaataaaa gggtgaatgc tttcaaaggc taaatgcagc caactgattg
     1441 cctctatggg atgaagtgcg gtcagttttt gttgatattt ttcaatcaat aattgcattt
     1501 cttcggcaat taaataaggg ggggggcatt ttctgcccct aaaattcgta ctggaatttt
     1561 gcgataaacc cctcggcttt ccgcattgcc cattaatacc agagaatgaa tatctttaat
     1621 cgctcgttcg cttaatggtt ctttattggc gactaattca taaatatagt tgaatgcatc
     1681 cttaaaaccg ataatatcta aatgctcccg aatcggtttt tcggcaatgg tcattccctc
     1741 ttttaaaatg agcaccgttt cacgcaaagt aatggtattg ccttcaattg cattggaatt
     1801 atagctgctt tcaataataa actcttcccg cagtcgttga acttcgcttt cagataatgg
     1861 acggcgttgt gaaagtgctg tttttaattg ttcgatttta gctaatttat tcattctaac
     1921 accatatcca aatgcgcaat cccatcttcc aaatacactt ctgaaaccgc tttaaaccca
     1981 aaactttcat aaaacccttg caggtaactt tgggcttggg cgtgaatggg ttgagcagga
     2041 aaatgttcgc cacaataatc catcgctttt tgtaccaatt cccttgctaa gcctgagcca
     2101 cgattttctt tcaccaccaa cactctacct atatgcacac catcattggc tggaattaaa
     2161 cggcagtaag cggtcagatt ttttgcattt tttgtaaaga aatgcaccgc ttgcaggtct
     2221 ttattatcga cttcttggta agggcagttt tgctcaacca caaacaccgc tgtacgtgcg
     2281 tggtaaatct caaacaactc ttgggtggag agttcatcaa agcgttttgc gcgccaagag
     2341 aggtttttta gcattgtatc gtttccagtt gccaaccaag ttggcggtat tgtttatagc
     2401 ggtcacgagc ctgtgctttg gcggtttctt ccaccggcac gaaatcaata atctgggtaa
     2461 aactgccgat aaattcgggg agttgggttt ggagtgaaat caacaaatcc cgacgttgag
     2521 cattgcgttt acctttccac gaaatttcga taggcgttgg gtaagtggtg atttctcccg
     2581 ataaattgtg cggcacgaac gaatccggat ctcttgccca aagtgcttcg tcaatattta
     2641 acgcttgttg ctcggtttcg catgcaatca gtacccgttt acctaatcgc cacgcttgag
     2701 ccgcaagttc gcaggcttgc atttcagcag cggaaagctc ggctgcatcg gcttggctta
     2761 atagataaaa ttgtacttgt ttcacagaat gttcccagaa attcaatcag atatttaaac
     2821 ttaatattct agctgaaaat gagtaaaata taaaatctaa gcgtttgttt ttgcaaaaaa
     2881 ttgagaaaaa atgaccactt gtgttgcgtt gccttgagga actaattagc ttttagacgt
     2941 tctaagagaa caatgcacta tttcaatagt tggaaattgt gtagaatacc cacttcattt
     3001 agctatttta taaggaactt actatgggct tatttgattt tgttggcgac attggtcgta
     3061 aattatttaa taaagaagaa gatgcttcaa aagcggttac agagcatttg gcggaagaca
     3121 atccgggcgt tgaaaatgta aacgtaacgg ttgaaaacgg ggtagcgaaa atttcaggca
     3181 ttgcttcaac cgcagcagct gttgagaaag cggtattgat ggcgggtaat gtggctggta
     3241 tcactgaggt aaatattgat gaagtgacct tgcctgatga cgtgcaactt gcaagtgatg
     3301 atgagcttta tgttatccaa aaaggggata cgttgtggga aatcgccaca aaagcttatg
     3361 gtaacggtgc aaaatataaa gcgattgtag aagcaaataa agaagttatt aaagacgaaa
     3421 ataaaatctt cccagggcaa aaaattcgta tcccgaaagg tttataatca aattgaaaga
     3481 gctaagctgg tcgaaagttt agctcttttt gtaggtggtg ttagactgaa aatggatatt
     3541 gaatggcagg gtggtattca taaccttcaa ctttgaaatc atctaagctc acccaagttt
     3601 ctaaatcttc taaggatttt atatcaggat taatgattaa tttaggtgca ttgagtggtt
     3661 ctcgcttaag ttgaacatca cgcattaatt ctagttgatc ttcataaata tgcgcattga
     3721 caattttatg gaacgcttgt cctgccttat gccctgtaat ttgagccatt aaagctaaaa
     3781 aggtatagca ttgaatcata ttccaattta acccaagcgg tacatctgcc gaacgctgag
     3841 tactattcag gtaaagcgtg ccatcaagta gagaaaaatg atggctgtgt aaacaaggac
     3901 gtaagcagcc catatggaaa gcgccggggt ggtagaaggt ataaatttca ccacggttat
     3961 caattccttt tttcaaatcg tccacaattt gtcgcaataa atcgactgag ccaccgtctg
     4021 gtttcgggaa attacgccct actgcgccgt aaaccaagcc catatcatct tccccttttc
     4081 gatagggatt agcaagccat tgtgcatttt catttgcatt agcgtcccac gattttgtgc
     4141 ctaattgacg gaaatcagca gcattactat agcctcgaat atagcctaaa agttcggcaa
     4201 tcgcagcttt ccaataacta cggcgagtgg taactaaagg aaattctcct ttttctacgt
     4261 cataagttaa gtcagcatta atgactgtga ggcagtgttt gcctgtacgt tcatttgcaa
     4321 cccatttgcc ttcgctaacg atgcggttac ataagtctaa atactgtttc attatttacc
     4381 tcataaaaaa ttttaggtaa ttttgccatt tattcggtaa aatagctaaa actttatgac
     4441 acaacacaat aaggagttat ggatatgaaa ggtataatga aatccattct ggttttttcc
     4501 accgctgttt ttttagcagc ttgttcatcg aataaaagcg gttggacacg tttaaatgat
     4561 aatgatgttg atcagaaatc ttatgctatt gcttatggtg caacggctca gacttatgcg
     4621 gatagagtaa atgaaagcta tgatatcaat tcctttatga atggggtaaa tgattggtac
     4681 actaaaaagg ttacattgcc aattgagcaa attagagcaa taacgattaa tcgaatgtta
     4741 gaccataatg tgtatgctta ttatagtggt gtgatttatg ccgcagattt gcaggcaaat
     4801 ttcaatcatt tagatcctaa ttgttggggg cttgttcaac cagcgagtat gacccagggc
     4861 attcacgatg cgatgttaga tttacagaag aacaaagttc gtggagatga gtatatcaaa
     4921 aatggggttg agcaaatatt gcacctttgt gtaaaaacga ttgctgaaga tgaaaatgaa
     4981 tcgcaatcta aaggtaaaac caaaaagcat aaataagtta agctatgaca agcggtaaaa
     5041 tttttaaaaa attttaccgc ttttttataa ccgtatgaaa tgtttaatat ttattctatg
     5101 aaacttaaaa aaacagcgtt ttgtttattc aataggcaaa cactcgggat ttttcaattt
     5161 tcaggaaaaa gaatttgaca tctttttaaa ttctagtaat atggcgccca cagacacagt
     5221 gagtggtgag atggccgagc aggctgaagg cgctcccctg ctaagggagt atagggttaa
     5281 aaagctctat cgagggttcg aatccctctc tcaccgccat ttactttatc acgcacccgt
     5341 agctcagctg gatagagtac tcggctacga accgagcggt cagaggttcg aatcctctcg
     5401 ggtgcgccat tttaattttt actctattca aattaaaatg gttgctgata aaattaaata
     5461 aacttacaat tcaaata
//
LOCUS       DANLXF010000160         5462 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.160, whole genome shotgun sequence.
ACCESSION   DANLXF010000160 DANLXF010000000
VERSION     DANLXF010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5462
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.160"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..210)
                     /locus_tag="PXD66_001893"
     CDS             complement(<1..210)
                     /locus_tag="PXD66_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320164.1"
                     /translation="MSLLKKAQTFISIFNEVRAKNEAQRQSSNNLNVALKNAAEMRAE
                     MNGFSLKPQKRSTYFADHYGCGIHAV"
     gene            complement(331..648)
                     /locus_tag="PXD66_001894"
     CDS             complement(331..648)
                     /locus_tag="PXD66_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL6320165.1"
                     /translation="MLSRKEVLMTRRASMNLQQIIRNVVEYTYSYESGDKLSAEIHNT
                     LDNLSYFPRIGVIGSVENTRECFCRGYRIIYRETENQVQILAIIHCRQQYPTDYYRSA
                     FKA"
     gene            complement(641..799)
                     /locus_tag="PXD66_001895"
     CDS             complement(641..799)
                     /locus_tag="PXD66_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320166.1"
                     /translation="MTEEYQNFLIEQVKQGLDDIENGRVISLEESRQLCQKTIYETLQ
                     ELQGAVYA"
     gene            complement(853..1008)
                     /locus_tag="PXD66_001896"
     CDS             complement(853..1008)
                     /locus_tag="PXD66_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320167.1"
                     /translation="MLFKEKGYDEFLTQKIAKGRAELESGKGIPLEQATLLVQSTIEE
                     TAQELDD"
     gene            complement(1146..1403)
                     /locus_tag="PXD66_001897"
     CDS             complement(1146..1403)
                     /locus_tag="PXD66_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320168.1"
                     /translation="MICHADPEYVKYNYAGLWEKGSDGTFFYNYQQFMPEVEAKESLR
                     EQEPELDDGYNEEEYSEEEYSDENDASDENDNYDETYDDNF"
     gene            1417..1593
                     /locus_tag="PXD66_001898"
     CDS             1417..1593
                     /locus_tag="PXD66_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320169.1"
                     /translation="MAYVRIYQGEAYGWCAEKGEANTECPEALLVSLTGDIFEAVGGD
                     KLKGAKKWQPYKKE"
     gene            1597..1743
                     /locus_tag="PXD66_001899"
     CDS             1597..1743
                     /locus_tag="PXD66_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320170.1"
                     /translation="MDTTQKRVALFIIGLSVVMWLRKHNCCTVIFVLLLAALAFSYTM
                     EHLY"
     gene            1759..1965
                     /locus_tag="PXD66_001900"
     CDS             1759..1965
                     /locus_tag="PXD66_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320171.1"
                     /translation="MVKNVVHEEILHNRAELQQAVEKEEFCAKCDNSVVFHLRDNYHE
                     FTLGLDTILQCLHFAEEKGAIRIP"
     gene            1962..2738
                     /locus_tag="PXD66_001901"
     CDS             1962..2738
                     /locus_tag="PXD66_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320172.1"
                     /translation="MNIEYSLNHCQGDGVAFYGRLGTDDLIQLFNHLNPQKRKQKMFA
                     NLLTHIESWETHDDIGFEIIRNRFGYRYSHWNTMELYAKTADQLVFFNDSDARRNWYF
                     PTSKVYKYKGLWDEFIQDLEQYIKGTSREMESVGYQIIEASPYQTETVFKFNTENYLV
                     ELKRKPSEFYSELVDWAFGDMCDFEATIEQICQGQLCYADFEATVTDKKSEIQLGLAR
                     LSGISYSPNDRSFSGYRQELIGEAIASARHNAARFAYLHA"
     gene            2853..3265
                     /locus_tag="PXD66_001902"
                     /pseudo
     CDS             2853..3265
                     /locus_tag="PXD66_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484619.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
     gene            3279..3434
                     /locus_tag="PXD66_001903"
     CDS             3279..3434
                     /locus_tag="PXD66_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320173.1"
                     /translation="METRIVKVIYDDGDYFFTRINADEQAIRNYYAIGSSIGFDDIRK
                     IIAVEIL"
     gene            3514..4488
                     /locus_tag="PXD66_001904"
     CDS             3514..4488
                     /locus_tag="PXD66_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1738 domain-containing protein"
                     /protein_id="HDL6320174.1"
                     /translation="MNTQPKFDLYQHITDRIVVALEQGTAPWLKPWSHPECNMALPRN
                     AVSNRLYSGINTLLLWIATAEHGYKQCKWITAKKANELGGYIRKGEKATVVMNYRPDE
                     REKLDEEGNIVYDEEGNPEMERHFFINKHNLFNLDQCENLPVELYDPVSEPKQPKSEP
                     NQYDIFAEIRQIIKGMDLQVEMKPSNRAFYQPGEDKVVMPEMKQFANAQAFHSVLLHE
                     MTHATGANNRLNREGITSGKAKFGNKVYAFEELIAEMGGAFLCAYLGFDTVPQNAAYI
                     ESWIRVLKEDKRAIVRASGKAREACQYMLDALNVAQLADKYDVFEEAA"
     gene            4575..4772
                     /locus_tag="PXD66_001905"
     CDS             4575..4772
                     /locus_tag="PXD66_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320175.1"
                     /translation="MTKSTEIPRGHDNWVITGRDNDGKWHKERFLYYSRREAIQQFRR
                     KHNCVGKHSVIESITPQFGWY"
     gene            complement(5251..5439)
                     /locus_tag="PXD66_001906"
     CDS             complement(5251..5439)
                     /locus_tag="PXD66_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320176.1"
                     /translation="MLERGYVRIYQGKAYGWCAEQEDANTECPTALIVSLSNEIFEAT
                     GGDDISGAEKWEKYNSNF"
BASE COUNT         1629 a          977 c         1125 g         1731 t
ORIGIN      
        1 tactgcatga ataccacaac cataatggtc agcaaagtaa gtagaacgct tttgaggctt
       61 aagggagaac ccattcatct cagctctcat ttcagcagca tttttcaagg caacatttaa
      121 attgtttgaa gactggcgtt gagcttcatt tttagctcgc acttcattaa aaatagagat
      181 aaacgtttgt gcttttttaa gtagagacat tgtggttctc ctgttttttg cacctaatat
      241 tattgaacta tggtaggctt ggcgagcatt gccttgagtt ttaattcggc tacggtcatt
      301 tttcttttta tttccatttt atagctcctg ttatgctttg aatgcagagc gataatagtc
      361 ggttgggtat tgttgacgac aatggataat ggctaaaatt tgcacttgat tttctgtttc
      421 tcgatatata attctataac ctcggcaaaa gcattctcta gtattttcta ctgaacctat
      481 aacaccaatt cgtggaaaat aagaaagatt atccaaagtg ttatgaattt ccgcagataa
      541 tttatcacca ctttcatagc tataagtata ttccacaaca ttacgaatga tttgttgtaa
      601 attcatactt gctctgcgag tcattaacac ctcttttcga ctaagcatac acggctccct
      661 gtagctcttg caaggtttca tatatggttt tttgacacag ttgacggctt tcttctaatg
      721 aaataacacg tccgttttct atatcatcaa gtccttgttt tacttgctct attaagaagt
      781 tttggtattc ttctgtcatg gataactcct atcattagtt gtatatagat atattagcaa
      841 tgttacaaat tgttaatcgt ccagttcttg agcagtttct tcaatagttg attgaactaa
      901 caatgtggct tgctcaagag gaataccttt tccggattct aattcagctc tgccttttgc
      961 aattttttga gtaaggaact catcgtagcc tttttcttta aatagcattt tatgttcctt
     1021 tcacttatta caaggctttc aggcataggg attatgttct cctaaatgcg aaatattgga
     1081 ataatttacg cttaaagcaa tagagtgtca agagcagggt ttttggaagt gtggtttggg
     1141 aatatttaaa aattatcatc ataagtttca tcgtagttat cgttttcatc gctggcatca
     1201 ttttcatcgg aatattcttc ttcgctatat tcctcttcgt tatatccatc atcaagttca
     1261 ggttcttgtt cccttagtga ttctttagct tcaacttcag gcataaattg ctggtaattg
     1321 tagaaaaaag tgccatcgct gcctttttcc caaagtccgg cgtagttgta ttttacatat
     1381 tcaggatcag cgtggcatat catccctaat tgaggggtgg catatgttcg tatttaccaa
     1441 ggtgaggctt atggttggtg tgctgaaaaa ggagaggcaa atacagaatg ccctgaggcc
     1501 ttattggtga gtttaaccgg cgatattttt gaagcagtcg gcggagataa gctgaaaggt
     1561 gccaaaaaat ggcagcctta caagaaagaa tgacagatgg atacgacaca aaaacgtgtg
     1621 gctttattca tcattggatt atctgttgtg atgtggctaa gaaagcataa ctgctgtaca
     1681 gtgatatttg tattgttgtt ggcagcgtta gcatttagct acacgatgga acatttatat
     1741 tagctgggga actgaattat ggtaaagaat gtggttcatg aagagatatt acataaccgt
     1801 gcagaattac agcaagcggt tgaaaaagaa gaattttgtg caaaatgtga taactcagta
     1861 gtattccatt tacgtgataa ttatcacgaa tttacgcttg ggttagatac tatattgcag
     1921 tgtctgcatt ttgctgaaga gaagggagct atccggatac cttgaatatt gagtattcac
     1981 tcaatcattg ccaaggagat ggagtcgcat tttatggtcg acttggtaca gatgatctaa
     2041 tccagctttt caatcatctc aatccacaga aacgtaaaca gaaaatgttt gctaatttgc
     2101 taacgcacat tgagtcttgg gaaacccacg atgatattgg ttttgaaatt atacgaaatc
     2161 gctttgggta tcgttactcg cattggaata cgatggagct ttatgctaaa actgcagatc
     2221 agttagtgtt tttcaatgat agtgatgctc gaagaaactg gtattttccg acttcaaaag
     2281 tgtataaata caaaggcttg tgggatgagt ttattcagga tttagaacag tatattaagg
     2341 gtacttcccg tgaaatggaa tcggtcggtt atcaaattat tgaagcctcg ccgtatcaaa
     2401 cggaaaccgt tttcaaattt aatacggaaa actatctagt agaactgaaa cgtaaacctt
     2461 ctgagtttta ttcagagctt gttgattggg catttggtga tatgtgtgat tttgaagcaa
     2521 ctatagagca gatttgtcaa gggcaactct gttatgctga ttttgaagca actgttaccg
     2581 ataagaaaag tgaaattcag ttaggtcttg ctcgcttgag tggaataagt tactcgccga
     2641 atgaccgttc gtttagtggt tatcgacaag agcttattgg tgaggcgatt gcctctgctc
     2701 gccataatgc agcacgtttt gcctacttgc acgcctaatt tgcagatttg tacttcaatt
     2761 tgaccgcttg tagtggttaa ttttcacccc gtggggaaat attttcccta ttgggcaaga
     2821 tgtttctcca ctttttaatt tggagaaatg tgatgaaaaa tgaacacaat ttagagcaac
     2881 ttaaatatat ttgggataac cgcaaccttg aaaataaagc ctatcaatac ctgtttcaca
     2941 tttgcattga gccgatgaaa aaaggagatt ggaatcgtta tacgatttgg cttaattttc
     3001 acggcaactt aatcaacgtg tcagacgttg tatatcgagt tgtggaatct caaaagctca
     3061 aggatgacat caaaatgtgg tatgacaata ataccattgt cgtccgataa aagttgaaac
     3121 aacaggcttc atctcatatt tactgcacca gttctatcag gggatagcct atattttact
     3181 ggaacaggga atgattactg aaactgaagc agccgagaaa agttataact tcagagctgc
     3241 aaattgtttt caacgtttgc tttaatggag gtgagataat ggaaactcgc attgttaaag
     3301 ttatttacga tgatggtgat tatttcttta cccgaatcaa tgctgatgaa caggctatcc
     3361 gtaattacta cgccataggc agttcaatag gttttgatga tataagaaaa attatcgctg
     3421 ttgaaatttt ataaaccacc cataggggaa actatttccc catagggtga tagcgtttcc
     3481 ccatttttct acccgaactt tggagaaaca cttatgaaca ctcaacctaa atttgattta
     3541 taccaacaca tcactgaccg aattgttgta gcactggaac aaggtacagc cccgtggtta
     3601 aaaccgtgga gccatccgga atgcaatatg gcattacctc gcaatgcggt atctaaccgc
     3661 ttgtattccg gtatcaatac actcttactc tggattgcta ctgctgaaca tggttacaaa
     3721 cagtgcaagt ggattacagc aaaaaaagcc aatgaactcg gcggatatat ccgtaaaggg
     3781 gaaaaagcta ctgtggtgat gaactaccgt cctgatgaga gagagaaact cgatgaggaa
     3841 gggaatattg tgtatgacga ggaaggaaat ccggaaatgg aacgacactt tttcattaat
     3901 aagcacaatc tgttcaatct ggaccagtgt gaaaatttac cggtggaact gtacgatcct
     3961 gtatcagaac cgaaacaacc taaatctgag ccaaaccaat acgatatttt tgctgagatt
     4021 cggcagatta tcaaaggaat ggatttgcag gtggaaatga agcctagcaa tcgggcattt
     4081 tatcaaccag gtgaagataa ggtcgtaatg ccggagatga agcagtttgc aaatgctcaa
     4141 gcgtttcata gtgtattgtt acacgaaatg actcacgcaa cgggggcaaa taatcggctt
     4201 aatcgggaag gtattacttc cggaaaggct aaatttggaa acaaagtcta tgcttttgag
     4261 gagttgattg ctgaaatggg cggtgcattt ttatgtgcct accttggttt tgatactgtg
     4321 cctcaaaatg cggcgtatat tgaaagttgg attcgagtat tgaaagaaga taaacgggcg
     4381 attgtccgtg cttccggaaa agctcgtgaa gcctgccaat acatgttaga cgcattaaac
     4441 gtcgcacagt tggctgacaa atacgatgta tttgaagaag ctgcttaaca aatttgccga
     4501 aaggcatatc tcaaggggag acctatccct ttgggcatag tgtttcccta ttttttatta
     4561 aatggagaaa agctatgaca aaatcaactg aaatacctcg tggacatgat aactgggtta
     4621 ttacaggcag agacaatgat gggaaatggc ataaagaacg ttttctttat tattctcgcc
     4681 gtgaagccat ccaacagttt cgtcgaaaac acaactgcgt aggtaaacat agtgttattg
     4741 aatcaataac acctcaattt ggctggtatt aaccggcttt cccgtgggga gatactctcc
     4801 cttcggagca ttgtgtctcc ctttttattc acttaactag aggacacaat gttcaatcta
     4861 aacttattta aacgccctat tgagctttca acgcttgaat cttggtctaa attctgtgat
     4921 gatgtcggta aagttgcatt tattgcgata cctccgatga tttatagtaa ttatgatcta
     4981 tgtatcaaaa taggaaacat aagtgcttta ttggcaatta cctatggatt cttattatca
     5041 ggcaggacta tccgtcaatt tattgagaag gagaaatcaa gatgggatta acaatcggct
     5101 tatggatcat tgcttttata ggctttgcac tggttttttg ggcaaatagc ttactcaaag
     5161 agcaagatga acgccttaaa cagctaaaaa aatagctcaa ctttacactc aatacggcac
     5221 atttggtgcc gtatttcatt cttatcctaa ttaaaaatta gaattatatt tttcccattt
     5281 ttcagcccca cttatatcat cacctccagt tgcctcaaaa atttcattag ataagctgac
     5341 aattaaggcg gtagggcatt ccgtgtttgc atcttcttgc tctgcacacc agccataggc
     5401 ttttccctga taaatacgaa cataaccacg ctctagcata aaagggtgag aatccttgtg
     5461 at
//
LOCUS       DANLXF010000161         5437 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.161, whole genome shotgun sequence.
ACCESSION   DANLXF010000161 DANLXF010000000
VERSION     DANLXF010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5437
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.161"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            153..1787
                     /locus_tag="PXD66_001907"
     CDS             153..1787
                     /locus_tag="PXD66_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005700110.1"
                     /note="is involved in the transport of the murein peptide
                     L-alanyl-gamma-D-glutamyl-meso-diaminopimelate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HDL6320177.1"
                     /translation="MQSKFSLSLLTTSLVLGLSANVFAAKVPEGTTLAEKQEITINNG
                     FEPSSFDPHKIEGVPESQVAYQLFEGLVTKDSDGNIQPGVAESWENTPDYKTWTFKLR
                     QDAKWSNGDAVTAHDFVFAWQRLVNPLTASPYSSYLTYLNVENAQDIIDGKKKPEELA
                     VKAIDDHTFQVQLSQPVPYAVELATHSSLLPVHKATVEKHGDAWVQKGNLVGNGAYTL
                     ADHVINEKIVFERNKNYWNDKETVINKATFLAIANAATDVSRYRAGDLDMTNYALPPE
                     QFATLKKEIPNEVFTARTLSTYYYEINHKRAPFDNVKVREALNLALDRTIITDKVLGQ
                     GQTPTYVFTPPYIHEGEKIQQPAYSKDSMADRKAKALKLLEEAGYSKANPLKFTILYN
                     TNDNHKKIAIAVSSLWKQGTDGIVDVKLENQEWKTFLDTRRNANYDVSRAGWSADYNQ
                     ATTFVNYFLSNSSNNTAFYKSQAYDDAVAESYKATDAAGRAAAYAKAEEQLAKDYAII
                     PIYNYVNPRLVKPYVKGYEGKDPQDDILLRNLYIVK"
     gene            1841..2113
                     /locus_tag="PXD66_001908"
     CDS             1841..2113
                     /locus_tag="PXD66_001908"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDL6320178.1"
                     /translation="MVAKLFSVYGRVQGVAFRFFTLQEANRLGIKGYVRNRDDGSVEV
                     VAEADEQTIAQLENWLQQGSPAAKVERVIVSDYLGSERFKRFEVRY"
     gene            complement(2173..4785)
                     /gene="pepN"
                     /locus_tag="PXD66_001909"
     CDS             complement(2173..4785)
                     /gene="pepN"
                     /locus_tag="PXD66_001909"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HDL6320179.1"
                     /translation="MQPKAKLRKDYRAPDFTITDIYLDFQLDPDRTFVTSKLTVVRKN
                     PEATTLRLDGHSFDFLSLKYNGEPFSAFQKDDESLTLNLADVPAERFELEIETALNPA
                     QNTSLQGLYKSGDGFCTQCEAEGFRQITYMLDRPDVLAKYTTKITASKSKYPYLLSNG
                     NHIAQGDLHDGRHWVEWQDPFFKPSYLFALVAGDFDLLQDKFITKSGREVTLEIYVDR
                     GNLDRAPWAMESLKRSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKF
                     VLAKPETATDTDYLDIEAVIAHEYFHNWTGNRITCRDWFQLSLKEGLTVFRDQEFTSD
                     LWSRSAKRIEDVRLLRTVQFAEDASPMAHPIRPEKVIEMNNFYTVTVYEKGAEIIRMI
                     HTLLGEEKFQQGMQLYVARHDGSAATCEDFVKAMEDASGVNLEQFRRWYSQSGTPELT
                     ISDEYDETRKAYRLHISQMTPATHDQMDKLNLHIPLKVALYGEQDGKRIPLQYELLTV
                     SDVLDVTAEHQTFEFHNVTQRPVPALLCDFSAPVRLDYNYTTEQLLTLLKHAENDFVR
                     WDAAQMLYTNELRENLSRYQQHQPLSFSEGLVNALTFVLDNSASLPELTALTLTLPKE
                     TEFAESFKTIDPQGIAVVREFMQHSIAHALQDKLLAVYHQNQCGEYQVVAEDIAKRAL
                     RNACLSYLAFTDLGNALVHKHYNNADNMTDTLAALQAATKAQLSCRDALLADFEQKWK
                     HDGLVMDKWFMLQATRPDDDVLSIVQNLMEHPSFNFNNPNRLRSLVGAFCGQNPKAFH
                     AIDGSGYRFLVDTLIKLNESNPQVAARLIEPLIKLSRYDNQRQTLMRRGLERLRELDN
                     LARDLFEKIEKALD"
     gene            complement(4935..>5437)
                     /gene="hutZ"
                     /locus_tag="PXD66_001910"
     CDS             complement(4935..>5437)
                     /gene="hutZ"
                     /locus_tag="PXD66_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heme utilization protein HutZ"
                     /protein_id="HDL6320180.1"
                     /translation="EVLQNRLGPEIEELKQQIKTIMLATIDKDGVPNVSYAPFVINNG
                     EYQVLITTIARHARNLQEVPKVSLMLIEDESKSRQIFARRRLTFDATVRMIERTDEEW
                     NNSIAALKARHGDLVDELSNMQDFKLFSFKPTAGLFVKGFGKAFDVGVDDLINVVHLD
                     QGHQTA"
BASE COUNT         1602 a         1085 c         1165 g         1585 t
ORIGIN      
        1 atttttttat gcaatttttg cattgaaagg tgatatctat cacatttttt aaaaaaattc
       61 cttgcaatca aaataataat gcctagactt aaaaaaatta attagaacaa atcggctggt
      121 tgcaagccaa tattcatcaa tggagatcaa atatgcagtc aaaattctcg ctttccttat
      181 taacaacctc attagtgtta ggcttatcgg caaatgtgtt tgcagccaaa gtgcctgaag
      241 gaacaacgct tgcagaaaaa caagaaatta cgattaataa tggttttgag ccatcaagtt
      301 ttgatccaca taaaattgag ggcgtgccgg aatcgcaagt tgcttatcag ttatttgaag
      361 gattggtaac gaaagattct gacggcaata ttcagccggg tgtggcagaa agttgggaaa
      421 atacaccgga ttacaaaacg tggacgttca aattacgcca agacgctaaa tggtcaaacg
      481 gtgatgccgt aactgctcac gatttcgtat ttgcttggca gcgtttggtt aatccactta
      541 cggcttcacc ttattcaagc tatttaactt atctcaatgt agaaaatgct caagatatta
      601 ttgacggcaa gaaaaaaccg gaggaactag cagttaaagc gattgatgat catactttcc
      661 aggtgcagtt aagccaacct gtcccgtatg cagttgagct ggcaacacac tcttctttat
      721 tacctgtaca caaagcaacc gttgagaaac acggcgatgc gtgggtgcaa aaaggcaatc
      781 tggttggaaa cggggcttat accttagccg atcacgttat taatgaaaaa atcgtgtttg
      841 agcgtaataa aaattattgg aatgataaag aaaccgtgat taataaagcg actttcttag
      901 ccattgctaa tgccgcaact gatgtttcac gttaccgtgc cggtgatttg gatatgacca
      961 actatgctct tccaccggag cagtttgcaa ccttgaaaaa agagattcca aacgaagtgt
     1021 ttacggcaag aaccctttct acttattact atgaaattaa ccataaacga gcaccgtttg
     1081 ataatgtgaa agtgcgtgag gcgttaaact tagctttaga tcgcaccatt attaccgata
     1141 aagtgctagg acaaggtcaa acaccaactt atgtatttac accgccttat attcacgagg
     1201 gtgaaaaaat ccagcaacct gcttattcca aagattcaat ggcagataga aaagccaaag
     1261 cgttgaaatt attggaagaa gccggttaca gtaaagcgaa cccgttaaaa tttactattc
     1321 tttataatac caacgacaac cacaagaaaa ttgcgattgc agtttcatca ttatggaaac
     1381 aaggcacaga cggcattgtt gatgtgaaat tagaaaacca agagtggaaa acattcttag
     1441 atacccgccg caatgcaaat tatgatgttt ctcgtgcagg ttggtcggca gactataacc
     1501 aagcaaccac ctttgtgaac tatttcttat cgaactccag caacaatact gcgttttata
     1561 agagccaagc ttatgatgat gcggtagcag aatcttacaa agcgacagac gcagctggtc
     1621 gtgcagctgc ttatgcaaaa gcagaggagc agttagccaa agattatgcg attattccga
     1681 tttataacta tgtaaaccca cgtttggtga aaccttacgt gaaaggctat gagggtaaag
     1741 atccgcaaga tgatatttta ctgcgtaacc tctacatcgt gaaataagac attatgggct
     1801 gtttattgaa aaataagcag cctattttta tgggggaaga atggtcgcaa aattattctc
     1861 ggtttacgga cgggttcaag gtgtcgcttt tcggtttttt accttgcagg aagcgaatcg
     1921 attaggcata aaaggctatg tcagaaatcg tgatgacggc tcggtagaag tggtagctga
     1981 agcggatgaa cagacaatcg ctcaactgga aaattggtta caacaaggct cacctgctgc
     2041 aaaggtggaa cgggtaattg tgagcgatta tcttggctca gaaaggttta aaaggtttga
     2101 ggtaaggtat taactaacaa gcggtgtcat ttgtaaaaaa atttgcaaac gacaccgctt
     2161 gtattgcttg gtttaatcta acgctttttc aattttctca aacagatctc gtgctaaatt
     2221 atccagctca cgcaggcgtt ctagaccacg acgcatcaag gtttggcgtt ggttatcgta
     2281 gcgtgagagt ttaattaacg gctcaataag tcgagcagca acttgtgggt tactttcgtt
     2341 aagtttaatc agcgtatcta ccaagaaacg atagcctgaa ccgtcaatgg cgtggaaggc
     2401 tttcgggttt tgaccgcaga atgcacctac taatgaacgt aaacggttcg ggttattaaa
     2461 gttaaaactt gggtgttcca ttaaattttg cacgatggat aacacatcgt cgtccggtcg
     2521 ggtggcttgt agcataaacc atttatccat cactaaacca tcatgtttcc atttttgctc
     2581 aaaatcggct aataatgcat cacggcaact tagctgggct ttggttgcgg cttgtaatgc
     2641 agcaagcgta tcagtcatat tatcggcatt attgtaatgc ttatgcacca acgcgttgcc
     2701 taaatcagta aaggctaaat agcttaagca agcattacgc aaggctcgtt tagcaatatc
     2761 ttcagccacg acttgatatt ccccgcattg gttttggtga tataccgcta ataacttatc
     2821 ttgcaaggcg tgagcaatgg aatgctgcat aaattcacgc acaaccgcaa taccttgtgg
     2881 gtcaatggtt ttgaacgatt ctgcaaactc ggtttctttt ggcaatgtga gggttaatgc
     2941 agttagttcc ggcaggcttg ctgagttatc caacacgaac gttagggcat tcaccaaccc
     3001 ttctgagaag ctgagtggtt gatgttgctg ataacggctt aaattttcac gcagttcatt
     3061 ggtatagagc atttgagctg catcccagcg tacaaaatca ttttcagcgt gtttgagtaa
     3121 ggttaaaagc tgctcggtgg tatagttgta atctaaccgt accggtgcag agaaatcaca
     3181 cagtaatgca ggcaccgggc gttgtgttac attatgaaac tcaaaggttt ggtgttctgc
     3241 ggttacatct aacacatcgc ttacggtcaa taattcatat tgtaacggaa tgcgtttacc
     3301 gtcctgttca ccatataacg ctactttaag cggaatatgc agattgagtt tatccatctg
     3361 atcgtgggtt gccggtgtca tttgtgaaat atgcaagcgg tatgccttgc gggtttcatc
     3421 atattcatcg gaaatggtga gctcaggcgt gccggattgg ctataccaac ggcggaattg
     3481 ttcaagatta acgcctgaag catcttccat tgctttaacg aaatcttcac aagttgccgc
     3541 agaaccatcg tgtcttgcca cataaagttg cataccttgc tggaattttt cttcgcctaa
     3601 cagggtgtga atcatacgaa taatttccgc ccctttttca tacacagtca cggtgtagaa
     3661 attattcatt tcaatcactt tttccgggcg aatcgggtga gccatcgggc tggcatcttc
     3721 ggcaaattgt acggtacgca gtaaacgcac atcttcaatc cgttttgccg aacgagacca
     3781 taaatcggag gtaaattcct gatcacggaa gacggttaag ccctctttta agcttagttg
     3841 gaaccaatca cggcaggtaa tgcggttgcc cgtccaatta tggaagtatt cgtgggcaat
     3901 caccgcttca atatctaaat aatccgtatc ggttgcggtt tccggttttg ccaatacaaa
     3961 tttggaattg aaaatattta accctttgtt ttccattgct cccatattga agaaatcaac
     4021 ggcaacaatc atataaatat ctaaatcgta ttccaaaccg aaacgctctt catcccattt
     4081 cattgaacgt tttaagctct ccatcgccca aggtgcacgg tctaaatttc cacgatcgac
     4141 ataaatttca agcgtgactt cacgtccgct tttggtgatg aatttatctt ggagtaagtc
     4201 aaaatccccc gcaaccaagg caaataaata gcttggttta aagaacggat cttgccattc
     4261 cacccagtgt ctgccatcgt gcaagtcgcc ttgggcaatg tggttgccgt tagaaagcag
     4321 atacgggtat ttgctcttac tagcggtgat ttttgtcgta tattttgcaa gtacatcagg
     4381 gcggtcgagc atataggtaa tctggcggaa accttcagcc tcacactggg tgcaaaaccc
     4441 gtcgcccgat ttatacaaac cttgtaacga ggtgttttgt gccggattta atgcggtttc
     4501 aatttcaagc tcgaaccgct cggcaggcac atcggctaaa tttaaggtta aagattcatc
     4561 atctttttga aaggcggaaa acggctcgcc gttatattta agcgaaagga aatcgaaact
     4621 atgaccgtct aaacgaagtg ttgtcgcctc aggattttta cgcacgacag ttagttttga
     4681 ggtgacaaaa gtgcgatctg ggtcaagttg gaagtcaaga taaatatcag taatagtgaa
     4741 atcgggagct cgataatctt ttctgagttt cgctttaggt tgcataggat ttcctttttt
     4801 tagttgcgtt tagctcatag aatagtgtcg ttattgtact aaaatagcgg taaattttcc
     4861 aaaaaatttg accgcttgtc gcaataaaaa cggctataaa taggattatt tatagccgtg
     4921 agtttatttc gcaattaggc agtttggtgg ccttggtcta aatgaacgac attaattaag
     4981 tcatcaacac caacatcaaa ggctttacca aatcctttta caaataagcc tgccgttggc
     5041 ttaaagctaa agagcttgaa atcttgcata tttgacagtt catcaactaa atcgccgtga
     5101 cgagctttaa gtgctgcaat gctattattc cattcctcat cagtacgttc aatcattcgc
     5161 actgttgcgt caaaggttaa gcgacgacgg gcaaaaattt gacggctctt actttcatcc
     5221 tcaattagca ttaaagagac ttttggcacc tcttgtaaat tacgagcgtg acgggcaata
     5281 gtagtgatta agacttgata ttcgccgtta ttaattacaa aaggggcata gctcacattc
     5341 ggtacaccat ctttatctat ggttgcaagc attatagttt tgatttgttg ttttaattct
     5401 tcaatttctg gacctaaacg gttttgtaag acctctt
//
LOCUS       DANLXF010000162         5370 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.162, whole genome shotgun sequence.
ACCESSION   DANLXF010000162 DANLXF010000000
VERSION     DANLXF010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5370
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.162"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            50..808
                     /gene="ftsN"
                     /locus_tag="PXD66_001911"
     CDS             50..808
                     /gene="ftsN"
                     /locus_tag="PXD66_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HDL6320181.1"
                     /translation="MLMRIIGVILFFGIGLWFLKSSAPVPTVPSAPQPANSQTKSELP
                     SRPEETWSYIRDLETRTVITDNSQTSQERLAQLTAEQKQQIEERRKQEEARLAAEQAQ
                     QKPATDVSATELAPKTEEVKSLTEEELAAKKAQEQALAQKQAEEKRRQAELKKQQEAK
                     KEQPAKIVASAATLADKAANQAGKFGLQCGAFKNKAQAENMQARLAMAGFNARIASSA
                     DWNRVFVGPIGDRAATSRAQSNARSVAECVIVAM"
     gene            complement(850..2031)
                     /locus_tag="PXD66_001912"
     CDS             complement(850..2031)
                     /locus_tag="PXD66_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HDL6320182.1"
                     /translation="MKTELDMIVIGGGMIGAATALGLAKQGLRLALLEKNPLPTFNPD
                     SDYDLRISAISMGSIQLLEKLGVWEQIVAMRSTPYTQLETWEIDGFNTLFSAADLGLE
                     KLGDMVENNVIQIALWEQLKQYPNCLQAVGFEQIFANKAGEYWEVTVDGNTFAAPLLI
                     AADGANSQVRQWAGIGLTAWQYRQHCLLATVTTEKPAPSGTWQQFYPTGPRAFLPLSG
                     NHGCVVWYDSPQKIAELKMLPAEKLGVAIEQHFPSRLNAELGNIQVQASGAFPLTRQH
                     AQHYVQNGVVLVGDAAHTINPLAGQGVNLGFKDVKTLLEVIEQAVKKGKNFASEQVLQ
                     RYEQKRKPDNLLMQTGMDVFYKAFKTDLLPLKIARNVGLVLADKVSPIKKQALKYAIG
                     I"
     gene            2138..2476
                     /locus_tag="PXD66_001913"
     CDS             2138..2476
                     /locus_tag="PXD66_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320183.1"
                     /translation="MSCKTHANHEHEHGEGCGHTAIKHNDHIDYLHDGHLHHNKHGDH
                     YDEHVLEVNETNPADCNAAHCCGDHVHGEVCGHEAVPHGDHIDYIVNGRLHHQHGDHC
                     DDHGPVEIVK"
     gene            2608..3477
                     /locus_tag="PXD66_001914"
     CDS             2608..3477
                     /locus_tag="PXD66_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HDL6320184.1"
                     /translation="MTLAIVYSRASIGVEAPLVTIEVHISNGQPKLTIVGLPEATVKE
                     AGDRVRSALLNANFIYPPQRITINLAPADLPKEGGRFDLPIAIGILAASGQIDSDLLT
                     QFEFLGELALTGHLRGVHGTIPAVISADKAKRQMILAKQNANEASLVSNATTYFAGSL
                     LEVVNMLNKRDKLPICQHISQHSAEIRPLVSRDLTDIIGQQHAKRALMIAAAGQHNLL
                     FLGPPGTGKTMLASRLADLLPEMTDEEAIETASVTSLVQNELNFQNWKQRPFRSPHHS
                     ASMVALVGGGCEN"
     gene            3569..>5370
                     /locus_tag="PXD66_001915"
     CDS             3569..>5370
                     /locus_tag="PXD66_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320185.1"
                     /translation="MFYLNNDNFKREPYRGYCPSHLEKLFTKPHLDIRGNGYYYPDNE
                     YVVAWYESDFIFKSRKAQAHAKVKLVNLTCPKYTGKNSAEYLSVLYISVPLNYLHYFT
                     IGSIWKGGVAKEQFALEEFYITVEAENQDGKRENLSIVSFGESEENGLEKPFDYTIYT
                     IPTEYHNYGDDLNRLLSISYQNQKFIIHPLHIFMMHYGYSTDIKRILATYPLDEVRER
                     LFIDKVVENFDVERYVVLPKYFVKKDAIFLYHFKYDEETTGTRVKKLVSQFRLNIHNQ
                     THPIEIDFWHTQKVELKIRGVRLGNAVLCAEIIGLNQPEGEDITLVLSQSKREKSGNK
                     TEEQNNEVNNVPITRVYTREPELDELPLTDNSPDNRTVEYNKRQFELLGRQRQIRALK
                     KAREESNQKMKALTPDEIDSLGVGESDGRNGKVGLAFCFLDDTPVGKSSKLYKLWQHA
                     KAVAEWNHGEAHWYTPNLGFRNDDELLPVSLETNKCFDYPEIAIIIRLQIYGETFFLI
                     DFSMKQRDISIRGLGYKPDPNEDFLYESDMSKSELSELLSAVHHHEHLPKEYIEKKNK
                     EKTKLTTFNHSEAESSNWAYNAVQKLTSRAITKPT"
BASE COUNT         1639 a         1173 c         1178 g         1380 t
ORIGIN      
        1 caacgagaaa aaaagtaaaa aaaatcaaaa agaaagtaat gttaaaaaga ttttaatgcg
       61 gattatcggg gtaattttat tcttcggtat tggtttatgg tttttgaaat ccagtgcacc
      121 tgtaccaaca gttccgtcag caccgcagcc tgctaattca cagactaaaa gcgaattacc
      181 aagtcgaccg gaagagacgt ggagctatat tcgtgattta gaaacccgta cggtaattac
      241 cgataatagc caaacttcac aagaacgttt ggcacaatta acggcggaac aaaagcagca
      301 aattgaagaa agacgcaaac aagaagaagc acgcttagcc gctgagcaag ctcaacaaaa
      361 gcctgcgacc gatgtatctg ctacagaatt ggctccgaaa acagaggaag ttaagtcgtt
      421 aacggaagaa gaattagcag caaaaaaagc acaggagcaa gctttagcac aaaagcaagc
      481 ggaagagaaa cgcagacaag cagagcttaa aaaacagcag gaagcgaaaa aagagcagcc
      541 ggctaaaatt gtagcaagtg ctgcaacact ggcagataaa gctgcaaatc aggcgggtaa
      601 atttgggtta cagtgcggtg catttaaaaa taaagcccaa gccgaaaata tgcaagcaag
      661 attggcaatg gcaggtttta acgcccgtat cgcaagcagt gccgattgga accgtgtttt
      721 tgtcggcccg ataggtgaca gagcggcaac ttccagagct caatccaatg cacgcagtgt
      781 agcggaatgt gtgattgtgg cgatgtaaaa ttgattgaaa tattgaagag agctataggc
      841 tctctttttt tatatcccaa tcgcatattt caaggcttgc tttttgatgg gtgacacttt
      901 atcagccaat actaaaccga cattgcgggc aattttgagc ggtaataaat cggtcttaaa
      961 cgctttataa aacacgtcca tgcctgtttg catcagtaaa ttatccggtt tacgtttttg
     1021 ctcatagcgt tgaagcacct gctcacttgc aaaatttttg ccttttttga ccgcttgttc
     1081 gatcacctca agcaaggttt taacatcttt aaagcctaaa tttacacctt gtccggcaag
     1141 cggattaatg gtgtgagccg catcgccgac taataccacc ccgttttgca catagtgctg
     1201 agcgtgctgg cgggttagcg ggaaagcccc tgaagcctgc acctgaatgt tgcctaattc
     1261 tgcgttgagc cggcttggga aatgttgctc aatcgcaact cccagctttt ctgctggaag
     1321 cattttcagt tcggcaattt tttgtggtga atcgtaccaa acaacgcagc cgtgattgcc
     1381 ggaaagcggt aaaaaggctc gagggccggt tggatagaat tgttgccaag tgccgcttgg
     1441 agcaggtttt tcggtggtaa cggtggcgag taaacagtgt tggcgatatt gccaagcggt
     1501 taaaccaatg cccgcccatt ggcgaacctg tgaatttgca ccgtctgcgg caatcagcag
     1561 tggggcagca aaggtgttgc catcaaccgt cacttcccaa tactcgcccg ctttatttgc
     1621 aaaaatttgc tcaaatccga ccgcttgtag gcagtttgga tattgcttta attgctccca
     1681 taacgcaatt tgaataacgt tgttttcgac catatcgccg agcttttcca aacccaaatc
     1741 agctgccgaa aaaagagtgt tgaatccatc aatttcccag gtttcgagct gggtataagg
     1801 ggtgcttctc attgcaacaa tttgctccca cacgcccaat ttttctaaca gttgaatcga
     1861 gcccatacta atggcggaaa ttcgcaaatc gtagtctgaa tcaggattaa aggtcggcag
     1921 agggtttttc tccaacaagg ctaaacgcag cccctgtttt gctaagccta gagctgttgc
     1981 tgccccaatc ataccgccac cgattacaat catatctaat tcagttttca ttttttcttc
     2041 ctttttagtt aggcaaaata ctgctaaaac gccttgaatt gtagtgcatt tcagattagg
     2101 atcaaaccga tttatctatt tatctaagga gcgttttatg tcttgcaaaa ctcatgcaaa
     2161 ccacgagcac gaacacggcg aaggctgtgg tcatactgct attaaacata acgaccatat
     2221 cgactattta cacgatgggc atttacacca caacaaacac ggcgatcact atgacgaaca
     2281 cgttttagaa gtgaatgaaa ccaacccggc agactgtaat gccgcccatt gctgcggcga
     2341 ccacgttcat ggtgaggttt gcggacacga agctgtgccg cacggcgatc atatcgacta
     2401 catcgtgaat gggcgtttgc accaccaaca cggcgaccac tgcgatgacc acgggccggt
     2461 tgaaattgtg aaataagatt tttgatggga ggttttagcc tcccattatt atttttgcga
     2521 tctcgctcga aaaattgaac attcctgcta cccctcttcc gattttcctt ttatgctctc
     2581 ggtattgatt tctaataccg aaatgtgatg accttagcga ttgtttacag ccgagcctcc
     2641 attggcgttg aagccccgtt ggtgacgatt gaagtgcata ttagcaacgg acaaccaaaa
     2701 ttaaccattg tcggcttgcc cgaagccaca gtgaaagagg ctggtgatcg tgtgcggagt
     2761 gcgttgctga acgctaattt tatctacccg ccacaacgga tcacgattaa tcttgctccg
     2821 gcagatttac ccaaagaagg cggacgtttt gacttgccga ttgcgattgg cattctggca
     2881 gcctcagggc aaattgattc tgatttatta acgcagttcg agtttttggg cgagttggca
     2941 ctgacagggc atttgcgagg agtgcacggc acgattccgg cagtgatttc tgccgataaa
     3001 gccaaacggc agatgatctt agccaagcaa aatgcgaatg aggcatcgct tgtttccaat
     3061 gccaccacct actttgccgg ctcgttgttg gaggtggtaa atatgctcaa caaacgggat
     3121 aaactgccga tttgtcagca catttcgcaa cattctgccg aaattcgacc gcttgtcagc
     3181 cgagatctta ctgatattat cgggcaacaa cacgccaagc gagccttaat gattgccgct
     3241 gccgggcaac ataatttgct gtttctcgga ccaccgggaa cgggtaaaac aatgttggca
     3301 agccggctgg cggatttact gccggaaatg accgatgagg aggcgattga aaccgcctcg
     3361 gtgaccagcc ttgtgcaaaa tgagctgaat tttcagaact ggaaacaacg cccgtttcgt
     3421 tcgccccatc acagtgcatc aatggtggca ttagtgggag gtggctgtga aaactagaaa
     3481 taaatgtcaa aaacaagaaa taaatgtcac aaatctttct ctaatttcgc tataattcga
     3541 tcatcaaaac tttaagggga aatccgttat gttctacctc aataacgaca atttcaaacg
     3601 agaaccttat cgtggatatt gtccgagtca ccttgaaaag ctttttacta aaccacacct
     3661 tgatattaga ggtaacggtt attattaccc tgacaatgaa tatgttgtag cttggtatga
     3721 atcggacttc attttcaaat caagaaaagc tcaagcacat gcaaaagtaa agcttgttaa
     3781 tttaacttgc cccaaataca ctggcaaaaa cagtgctgaa tatttaagcg ttttgtatat
     3841 ctccgttccc cttaattatc tacattattt cactatcggc agtatctgga aaggcggtgt
     3901 cgccaaagag cagtttgctc tcgaagaatt ttacattacg gttgaagctg aaaatcaaga
     3961 tggtaaacga gaaaacctct caatcgtttc attcggtgag agtgaggaaa acggtttaga
     4021 gaaaccattt gattacacaa tctacactat ccccacagaa tatcacaact acggtgatga
     4081 cctaaaccga ctactttcca tctcttacca aaatcaaaaa ttcattatcc atccgctaca
     4141 tatttttatg atgcactacg ggtactctac ggacatcaag cgtatcttgg caacataccc
     4201 acttgacgag gtaagagaac gcctatttat agataaggtc gttgagaatt ttgatgtaga
     4261 gcgttatgtc gttttaccga aatactttgt aaaaaaagat gcgattttcc tctatcactt
     4321 caaatatgac gaagagacta cgggaacaag ggtaaaaaag cttgtaagcc agtttaggtt
     4381 aaatattcac aatcaaacac atccaataga aatagatttt tggcacacgc aaaaggttga
     4441 attgaaaatc cgtggtgttc gacttggaaa tgctgtgtta tgcgcagaaa ttatcggtct
     4501 taatcagccc gaaggcgaag atattacgct agtgctatct caatcgaaaa gagaaaaatc
     4561 gggtaataaa accgaggaac aaaataacga ggtaaataac gttcctatta ccagagttta
     4621 tacacgagaa cctgaattgg atgagttacc actcaccgat aactccccag ataatcgaac
     4681 agtcgaatac aacaagcgac aatttgaact cttgggccgt caacgccaaa tcagagcgct
     4741 caagaaagca cgcgaagaga gtaatcagaa aatgaaagcc ttgactccag atgagataga
     4801 ctcactgggt gtaggtgaat cagatggccg aaacggtaag gttggactcg ctttctgttt
     4861 ccttgatgat acacctgttg gcaaaagctc caaactatac aaattatggc agcacgcgaa
     4921 ggctgttgcg gaatggaatc acggtgaggc gcactggtac acgcctaatc tcggttttag
     4981 aaatgacgat gaactccttc ccgtttcctt agaaaccaac aaatgtttcg actaccctga
     5041 aatcgcgata attatccgcc tccaaatata cggagaaaca tttttcctga ttgatttctc
     5101 aatgaagcaa agagatataa gcattcgtgg cttagggtat aaacccgatc caaacgaaga
     5161 ttttctctat gaaagtgata tgagcaaaag cgaattatcc gaattattgt ccgcagttca
     5221 tcatcatgaa catctgccaa aagagtacat agagaaaaag aacaaagaaa aaaccaaact
     5281 cactacattt aaccatagcg aagccgaaag tagtaactgg gcttataacg cggtgcaaaa
     5341 attaacttca agggcaatta ctaagccgac
//
LOCUS       DANLXF010000163         5362 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.163, whole genome shotgun sequence.
ACCESSION   DANLXF010000163 DANLXF010000000
VERSION     DANLXF010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5362
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.163"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            10..300
                     /locus_tag="PXD66_001916"
     CDS             10..300
                     /locus_tag="PXD66_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320186.1"
                     /translation="MEQLQAVSNEQVFAKLCEVERLLKTKGVNEHSRELWDLGDVAAY
                     FGYTKEHTSRSVVSSPHFPKPIALDGLRGKGRGAKKWVSGEVVKFCLMWKVK"
     gene            complement(570..752)
                     /locus_tag="PXD66_001917"
     CDS             complement(570..752)
                     /locus_tag="PXD66_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HDL6320187.1"
                     /translation="MAMSRNEIQAKSEAKRGIKQKSFKLPLEVIAEIEVLSQKLNIPQ
                     NQLIIQAIQQFKQNNP"
     gene            complement(1030..2028)
                     /locus_tag="PXD66_001918"
     CDS             complement(1030..2028)
                     /locus_tag="PXD66_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL6320188.1"
                     /translation="MATIIKNGNKYRAQVRKKGIYKTATFSTKTEANRWAYALEAQID
                     AGEYNTTPNILFAELIDKYLKEVTPTKRGKREERYRLLRIAGSNLGNIPLPALTKEHL
                     RQWQNQRLQEVQPVSVSRERATLSALFTKAMEWDYLKENPLKTLEKIKTPPARTRRYS
                     DEEIDKLIFVSGYSPNQPPLLMQQRAGAALLFAIETAMRAGEIAGLKWEHINLQARTA
                     FLPQTKNGYSRTVPLSTKAIAILNQLAQLEPNKQNSVFQLTSRSLDSNFRKLKQKANL
                     HNADLHFHDTRREALTRLSKYLTVMELAKVSGHRDLSILQNTYYNPDISELVSKLQ"
     gene            complement(2256..2332)
                     /locus_tag="PXD66_001919"
     tRNA            complement(2256..2332)
                     /locus_tag="PXD66_001919"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2296..2298),aa:Val,seq:gac)
     gene            complement(2396..3196)
                     /gene="apaH"
                     /locus_tag="PXD66_001920"
     CDS             complement(2396..3196)
                     /gene="apaH"
                     /locus_tag="PXD66_001920"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HDL6320189.1"
                     /translation="MATYIVGDLHGCFNEFQLLLEKVNYNPKQDELFLTGDLVARGED
                     SLACLRFVKDSANNAKTVLGNHDLHLLGIKRVKPNDKIDAIFEAKDRLDLQNWLRNQP
                     LVIHHPKHQFLLTHAGISPEWDLATTLACAKEAEKVLQSDNYADYIAQMYENKPERWS
                     EDLQGVERWRYIINVFTRMRFCYTDKRLDFDCKLPLEQAPQELLAWFDLDNPLFTQKN
                     IIFGHWASLMGKCTRPNIYALDTGCAWGSHLTMLRWEDKQIFTQKRLK"
     gene            complement(3288..5264)
                     /locus_tag="PXD66_001921"
     CDS             complement(3288..5264)
                     /locus_tag="PXD66_001921"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HDL6320190.1"
                     /translation="MFQNNPLLAQLKQQIEASKEYVAGVVKASDKAFGFLECDKKSYF
                     IPPAEMKKVMHGDSVKAVVKRDGDKEQVEIDSLLEPMLDRFIAQVRLNKEGKLQLAVD
                     HPSIKQTIPANTHKKATEKLENGDWVVAQLKTHPLRDDRFFFAQVTQFICKESDNFAP
                     WWVTLARHEQPREPVANEKNYELHDELAREDLSHLYFTTIDSPSTQDMDDALFIEPIK
                     QNGKRTGWRLVVAIADPTAYIPENSHIEKAARQRCFTNYLPGFNIPMLPRELSDDLCS
                     LVPNEKRPALVGFIETDLNGNLIKETTFTSAWVESKDRLAYDDVSDYLEGVENAWQPQ
                     SEETKQQIEWLHQFTQARIQWRAENALLFKEQGDYTFELNEDGSVKDIHVEYRRIANQ
                     MIEESMILANICCAKFLSQHAKTGVFNTHSGFDPKNYELVKSFLLQTLASDENRDELA
                     ERYSAERLATLEGYCQMQRDIQDYPEKFLELRLRRYLTFAEFKAEVAPHFGLGITDYA
                     TWTSPIRKYGDMVNHRLIKQVLLGKQSQAVEESVLSRLQEARRQNRLVERDIADWLYA
                     RYLAPMVEQAVEFECEIADVSRGGLRAKVIKNGAQIFVPFSSLHDNKEEMEYRQEELA
                     LYIKGEKAYQIGQAVKVKLTEVRLETRSIVGNII"
BASE COUNT         1421 a         1015 c         1196 g         1730 t
ORIGIN      
        1 gtggtaaaaa tggaacaatt acaagcggtc agtaatgagc aagtttttgc aaaactttgt
       61 gaggttgagc ggttattaaa aaccaaaggg gtgaatgagc atagccgtga attgtgggat
      121 ttgggcgatg tagcagcata tttcggctac actaaagaac acaccagccg aagtgttgta
      181 tctagtccac attttccgaa accgattgcc cttgacggct tacgaggcaa gggcaggggg
      241 gcgaagaagt gggtatccgg tgaagtggtg aaattttgtt tgatgtggaa ggtgaagtga
      301 tgataaaaaa gataatttta gatgcttgtt gcggtagccg aatgtttcac tttaataagc
      361 acaacccctc ggtattgttt gctgacaatc gggaatccct gtatcgaaaa ttttaaatgt
      421 tatcgaatac caacctatct atgggcataa aagcggtaaa cataacaaaa cgcattggct
      481 ggcatttgta aagatggata ataacgatta tttgtaaagt atttcaaaat acaaaagccg
      541 ataaatttca ttatttatcg gcttttgttt tatgggttgt tttgcttgaa ttgttgaata
      601 gcttgaataa tcagctggtt ttgtggaatg ttgagttttt ggctaagtac ttcgatttct
      661 gcgatcacct caagcggtag tttaaaggat ttttgcttta ttcctcgttt tgcctcgctt
      721 ttagcttgga tttcatttct tgacattgcc ataatttatc cctattataa attttgttga
      781 agagctggga gctttcaccc ccagcgagtt gcttcagatt agtaagctgg ggagctaagg
      841 actaataaga agacaactag gataatgtat ttaaacattg tgctatcctt cttcatttcg
      901 taaggtttaa gccttacgct cactttcaag ctgtctcttg aaagtgaggt tattataatg
      961 tagggtatcc tacattgcaa gtaataaata caaaaaagcc gataaatttt cgtttatcgg
     1021 cttttgtttt tattgcagtt tgctcacaag ttctgaaata tccgggttat agtaggtatt
     1081 ttgcaaaata cttaaatctc gatgccccga tactttggca agctccatta ccgtcagata
     1141 tttagacaaa cgtgtgagtg cctctcgccg tgtgtcgtga aaatgcagat cggcattgtg
     1201 tagattggct ttttgtttca gcttgcgaaa attcgaatca aggctgcgac tggtgagctg
     1261 gaaaacactg ttttgcttgt tcggttcaag ttgtgccaac tgatttaaaa ttgctattgc
     1321 tttggttgag agtggcacgg tgcgtgaata accgtttttg gtttgtggca aaaatgcggt
     1381 gcgtgcctgc aggtttatat gttcccattt taaaccagca atttcacccg ctcgcattgc
     1441 agtctcaatc gcaaataata gtgctgctcc ggctctttgt tgcattaata gcggtggctg
     1501 gttagggctg tagccggaga caaaaatcag tttgtctatc tcctcatcgc tatatcggcg
     1561 agttcttgct ggtggcgttt tgattttttc cagcgttttt agaggatttt ctttgagata
     1621 atcccattcc attgcttttg taaagagtgc cgataaagtg gctctttctc ttgagacgga
     1681 aaccggctgc acttcttgca agcgttggtt ttgccattgg cgtaaatgct ctttggtgag
     1741 tgccggtaat ggtatatttc ccaagttgct gccggcaatt cttagcaggc gatagcgttc
     1801 ttcccttttg ccacgtttgg tcggagtgac ctctttgagg tatttgtcga ttaattcggc
     1861 aaaaagaatg ttcggggtgg tgttatattc tccggcatca atttgagcct ctaaagcgta
     1921 agcccagcgg tttgcctctg ttttggtgct gaaagtggca gttttataaa tgcctttttt
     1981 gcggacttgt gctcggtatt tgttgccgtt cttaataata gtagccattt ttttattcct
     2041 ttgtgtatgt gtgcagttta agtgtgcaaa tgtgtgcaaa ggaatgttaa tagagatcat
     2101 aaacgatcaa taaatatcaa gttttcatat caaaaatgat gtttttattg atgttatgct
     2161 acacttgggg aagtgtttta aatcaataag ctattgattt ataaggggta tttttggggt
     2221 taagcctgaa tggggatatt caggcttaaa aataatggtg cgactagctg gactcgaacc
     2281 agtgaccccc accatgtcaa ggtggtgctc taaccaactg agctatagtc gcattaacgg
     2341 tgtgcattct attggaaaaa ggaacttagt gcaatatgct tttggtcgaa tgcttttatt
     2401 ttagacgctt ttgggtaaaa atttgtttat cttcccaacg gagcatggtt aaatggcttc
     2461 cccaagcaca gccggtatca agtgcataga tgtttggacg agtgcatttg cccattaggc
     2521 ttgcccagtg accaaagata atgtttttct gtgtaaataa cggattatct aagtcaaacc
     2581 aagccagcag ttcttgaggg gcttgttcaa gcggtaattt acagtcaaaa tctaaccgct
     2641 tgtcggtgta gcaaaaccgc atacgggtaa acacattgat gatataacgc caacgttcaa
     2701 caccttgcaa atcttcactc cagcgttcgg gcttattctc atacatttgg gcgatgtagt
     2761 ccgcataatt gtcactttgt agcacctttt ctgcctcttt ggcacaagct agggtagttg
     2821 ctaaatccca ctcagggctg attcctgcgt gagtcagcaa aaattgatgt ttggggtgat
     2881 gaattacaag cggttgattt ctgagccaat tttgcaaatc gagccgatct ttagcctcaa
     2941 aaatagcatc aatcttatca tttggtttta cccgtttgat gccaagcaag tgcaaatcgt
     3001 ggttgcctag cacggttttg gcattgtttg cagagtcttt cacaaaacgt aaacaggcta
     3061 aagagtcctc accacgggcg actaaatccc ctgttaaaaa gagttcgtct tgcttggggt
     3121 tataattgac tttttctaat aaaagctgaa attcgttaaa gcaaccgtgt aggtcgccta
     3181 caatataagt tgccattgtt tctcctttgt tcccctaaag ttataaacaa gcggttatat
     3241 tttagagaaa atttgcaaaa tctcatcaaa atataaccgc ttgaaattta aatgatattt
     3301 cctacgattg agcgggtttc taagcgtact tcggtgagtt tcactttcac cgcttgcccg
     3361 atctgatagg ctttttcacc tttaatataa agtgcaagtt cttcttggcg gtattccatt
     3421 tcctctttgt tgtcgtgcaa gctggagaat ggcacaaaaa tttgagcacc gttttttatg
     3481 actttcgcac gcaagccacc acgggaaaca tctgcaattt cgcactcaaa ttcgaccgct
     3541 tgttccacca ttggggcaag gtaacgagca taaagccagt cagcaatatc acgttctact
     3601 aaacggtttt gacggcgagc ttcttgtaaa cggcttaaaa ctgattcttc caccgcttgg
     3661 ctttgtttgc caagtaacac ttgcttgatt aaacggtggt ttaccatatc gccatattta
     3721 cggatgggtg atgtccaagt ggcgtaatcg gtaatgccta gtccgaaatg cggggcaact
     3781 tcggctttaa attcagcgaa ggttaaataa cgtcgtaaac gcaactctaa gaatttctct
     3841 gggtagtctt gaatatcacg ttgcatttgg caatagcctt ctaaggttgc taaacgctcg
     3901 gcggagtagc gttcggcaag ctcatcacga ttttcatcgc tggctaaagt ttggagtaaa
     3961 aatgatttta ccaattcata atttttcgga tcaaaacctg aatgggtatt aaatacaccg
     4021 gttttggcat gttggcttaa gaattttgca caacaaatat tggcaagaat cattgattct
     4081 tcgatcattt gattcgcaat acggcgatat tcaacgtgaa tatcttttac cgagccatct
     4141 tcatttaact caaaggtata atcgccttgt tctttgaata gcaacgcatt ctcggcacgc
     4201 cattgaatgc gggcttgagt aaattgatgt agccactcaa tttgttgttt tgtttcttca
     4261 gactgaggtt gccacgcatt ttccacgcct tcgagataat cggaaacatc gtcgtaagcc
     4321 aagcgatctt ttgattcgac ccaagcagag gtaaaggttg tctctttaat gagattgccg
     4381 tttaaatcgg tttcaataaa gccaacgagg gcagggcgtt tttcattcgg gacaagcgaa
     4441 cagagatcgt ctgagagttc tcgaggtaac attggaatgt taaagcccgg caaatagttg
     4501 gtgaaacaac gctggcgagc ggctttttca atatgggaat tttctgggat ataagcggtt
     4561 gggtcagcaa tcgctaccac taaacgccag ccggttcgct tgccattttg cttaattggt
     4621 tcgatgaaca gagcatcgtc catatcttgc gtgcttgggc tgtcaatagt ggtaaagtaa
     4681 agatgagata aatcttcacg agctaattca tcgtgtaatt catagttttt ttcatttgcg
     4741 accggttcac gaggctgttc gtggcgggca agtgtgaccc accaaggggc aaaattatca
     4801 gattctttac agataaattg ggtaacttga gcaaagaaaa aacggtcgtc acgcaatggg
     4861 tgcgttttta attgtgcaac cacccagtcg ccattttcta atttttcagt ggctttttta
     4921 tgggtatttg ccgggatagt ttgcttaatg cttgggtgat caaccgcaag ttgcaatttg
     4981 ccctctttat tcaagcgaac ttgggcaata aatctatcca gcattggctc aagtagtgaa
     5041 tcaatttcta cctgttcttt gtcgccatca cgtttgacta cggctttcac tgaatcaccg
     5101 tgcattactt ttttcatttc cgcaggtgga ataaaatagc tttttttatc gcattctaaa
     5161 aaaccgaatg ctttgtctga ggctttgaca acgcctgcga catattcttt gcttgcttca
     5221 atttgttgtt tgagttgggc taaaagggga ttgttttgaa acataatttc tcattttaaa
     5281 aataaaaaaa ggtgctatcg ttgcaccgaa aaaaacaatg gcattataac ggatttgaac
     5341 caagttcagc aaataaatgc tg
//
LOCUS       DANLXF010000164         5355 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.164, whole genome shotgun sequence.
ACCESSION   DANLXF010000164 DANLXF010000000
VERSION     DANLXF010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5355
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.164"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            277..1281
                     /locus_tag="PXD66_001922"
     CDS             277..1281
                     /locus_tag="PXD66_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL6320191.1"
                     /translation="MPGDANKIISNGTSAGGALSTLLGASADHYDYEPYLKEAGALNA
                     SDKIFAVSAYCPITNLENADMAYEWQFNGVNEYSRIDMSRLNAAEFNDRSKPKPKPKI
                     EGSLNEAEIKVSNELAERFPTYLNSLHLVDEKGNPLTLDPKGNGSFKDYLSEVVKTAA
                     NKAYRGLVQDSEEQKAFQQISWLSFEKGKVSSVDWFGYVFSDKRMKSPPAFDALNGSS
                     GENNLFGTDTENNRHFTLYSAERSANKDLNLADPQIVKRMNPMHYLDNPNAAEHWRIR
                     VGTADRDTSLAISAILAIKLQMAGKNVNYETPWNVPHSGDYDVNELFLWADELVKGKK
                     "
     gene            1336..3186
                     /locus_tag="PXD66_001923"
     CDS             1336..3186
                     /locus_tag="PXD66_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL6320192.1"
                     /translation="MQVKKFSQKYINWVLKLGRLKASLLGFFMVALSAILIQSSLTYL
                     FIGRVVVVDILRSIAFGLISAPFVLYFFTLLVEKLEISRLKLEKAVRDLEELREQDAY
                     LNAKLEKNSRDKSILMATISHELRTPLNGIIGLSQILLDDKTTEKQQEYLKTINMSAT
                     ALAHIFGDIIDLEKIDSSRIELVRKEVEFEQLIHDIANFGHFMAGNKQINFEVKYPPD
                     LPKYIYIDHARLSQILWNLISNAVKFTPASGRILLEIYQLDKEQFSFAVTDSGIGIPE
                     KDQRKIFDMFYQAENSKEKKAQGSGIGLSVSKRIAKLMGGDLVVASQENQGSTFTLTV
                     QAESTETKPIFAIQQHSLKVLLVEDIEMNVVVARAMLEKIGCVVDVAMSGEETFQHFA
                     QNSYDLILLDIQLPDTTGFEIAQQLRLQYENEEIDYLPLLVALTANVIQSRQEYQAQG
                     MDDVLHKPLSLDELMRCLNQHFGDDFLQKNEEKQPLVERGSENLPYSITILNELIGVM
                     GKENVLANMDLFADLMPTYLANLQRYLSDWQTTNSVESRKLTADEAHKIKGALASVGL
                     HQLQQTAQLAQSDNGEQWEQNIAHWVEMICSKWQMDLQLAKEYVRSKGDI"
     gene            complement(3282..3836)
                     /locus_tag="PXD66_001924"
     CDS             complement(3282..3836)
                     /locus_tag="PXD66_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDL6320193.1"
                     /translation="MKSQIYYDYYPSPIGQLLLLATEEGLIYIELGQEQQTTSLDNFL
                     SAQAASGQILEIFCKTKEILDRYFAGEPLDFQQLDFLAPQGTPFQQSVWKILREIPYG
                     QTTTYGEIATQLGKPSAMRAVGGAVGRNPISILVPCHRVLGKNQALTGFGGGLPNKRF
                     LLQLEDITYKDKGIEFVKPKTKKW"
     gene            complement(3903..5354)
                     /locus_tag="PXD66_001925"
     CDS             complement(3903..5354)
                     /locus_tag="PXD66_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014064876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HDL6320194.1"
                     /translation="MASLVAFSSAGASAAAFQLAEISTSGLGRAYAGNAAVADNASVV
                     ATNPALMTLFKQTEISAGGIFVDADVNIDGKLNATGADASHKNVIPTAIVPNAYIVSP
                     INERLSIGGGVNVNYGLISEFDPNYSAGFLGGRTSLSAVNVNFSAAYDLGYGFVLGAG
                     LNAVHAKAELGRFLGAGAARFAPAKQFLEDKKVQVQSAIQGLKALEAGIAAGNTALEA
                     QKTQVLAQVNGLLKSQNLNQTITNSAQLERLVNGIQNTNASSTIHKLKGDKWALGWNV
                     GLVYNINERNRLGFTYHSEIDLKFKGKYSNTVPVVIPKMVDTITGGAEIDGTLRLRLP
                     AFWEVSGYHKLTDKLAAQYSYKRTDWSKFQSLEAYDMQGNRLLHKTENFNDSQRIALG
                     LSYDVNEMLTLRTGVAFDESASVTHPSISIPDTDRAWYSVGATVRFTPNLSADVGYSH
                     LRGSNLTFSEDGLATFKSKARANLYGLNVNYKF"
BASE COUNT         1672 a          977 c         1151 g         1555 t
ORIGIN      
        1 agtcgatttt atgttcaaag agctgtaata agacggaata atgtcagacc aagatagata
       61 ctttcaagta aaatcaagca taaaaatgat tttcagactt tacaaatctg aaagaatgtg
      121 taagatcagg attgtccgaa attcggatgg aaaatcccag tggatgtatt ggctggatac
      181 atcataataa ctatgtattt gtgaatgtta tttgaatgat tctacgtagt tgattcaaaa
      241 tcagcagtgc ggtatctgca tgctaatgat gaaaaaatgc cgggcgatgc aaataaaatt
      301 atctctaacg gcaccagtgc cggaggagca ttatctactt tgttaggagc ttcagctgat
      361 cattacgact atgagcctta tttgaaagag gcgggggcat tgaatgccag tgacaaaatt
      421 tttgcagttt ctgcctattg cccaattacc aaccttgaaa atgcggatat ggcgtatgaa
      481 tggcaattta atggtgtcaa tgaatatagc cgaatcgata tgagtcgatt aaatgcagca
      541 gagtttaacg accgctcaaa accaaaacca aagccgaaaa ttgaaggctc gttaaatgag
      601 gcggaaatta aagtttctaa tgaattagca gaacgctttc cgacttattt gaacagtttg
      661 catttggttg atgagaaagg caatccttta acacttgatc caaaaggcaa cggctcgttt
      721 aaagattatt tatctgaagt ggtaaaaacg gctgcaaata aagcctatag aggtttagtt
      781 caagatagcg aagagcaaaa agcctttcaa caaattagtt ggctttcatt tgagaaaggc
      841 aaagtcagca gtgtggactg gtttggttat gtgttttcag ataaacggat gaaatcacca
      901 cctgcatttg atgcgttaaa tggaagttcg ggcgagaata atttatttgg cacagacacg
      961 gaaaataacc gccattttac gctttatagt gcggaacgtt ctgccaataa agatctcaat
     1021 ttagccgatc cgcaaatcgt gaaacggatg aacccgatgc actatttgga taacccaaat
     1081 gctgcggaac attggcggat tagggtggga acggcagata gagatacctc gctggcaatt
     1141 tctgcaatct tagcaataaa actgcaaatg gcaggcaaaa atgttaatta tgaaaccccg
     1201 tggaatgtac cccattcagg tgattatgat gtaaatgaat tattcctatg ggctgatgag
     1261 ctagtcaaag gcaaaaagta aaaaatagag taaaataaag cagatctgat ggtctgcttt
     1321 ttgttttgag gtcgtatgca agttaaaaaa tttagccaaa aatatattaa ttgggtatta
     1381 aagctagggc ggttaaaagc ttctctttta ggctttttta tggtcgctct ttcggcaatt
     1441 ttaattcaaa gtagcttaac ctatcttttt attgggcgag tagtcgtggt ggatatttta
     1501 cgctccatcg cttttggttt aatttctgcg ccttttgtgc tttatttctt cacgttattg
     1561 gtggaaaaac ttgaaatatc tcgcttgaaa ttagaaaaag cagtaagaga tttggaagaa
     1621 ctccgggaac aagatgccta tcttaacgct aaattagaaa aaaacagccg tgataaaagc
     1681 attttaatgg cgaccattag tcacgaactt cgtactcccc ttaatggaat tatcggcttg
     1741 agccaaattt tgttagatga taaaaccacc gaaaaacagc aagaatatct aaaaactatc
     1801 aatatgagtg ccactgcatt ggctcatatt tttggcgata ttattgattt agaaaaaatt
     1861 gatagtagcc gaattgaatt ggtaagaaaa gaggtggaat ttgaacaact cattcacgat
     1921 attgctaatt ttgggcattt tatggcgggc aataagcaga ttaattttga agtgaagtat
     1981 ccgcctgatt tgccgaagta tatctatatc gaccacgcac gcttgagcca aattttatgg
     2041 aatttgatca gcaatgcggt gaaatttacc cctgcaagcg gtcgaatttt gctggaaatt
     2101 taccaattag ataaagaaca gtttagcttt gcggtgacgg attcaggcat cggtattccg
     2161 gaaaaagatc aacgcaaaat ttttgatatg ttctaccaag cggaaaactc taaagagaaa
     2221 aaagcgcaag gtagcgggat tggcttatcg gtctcgaaac gcattgcgaa gctaatgggc
     2281 ggtgatttgg tggtggcaag ccaagaaaat caaggctcaa cttttaccct cactgtgcag
     2341 gcagaaagca cggaaaccaa gccgatattt gctattcagc aacattccct taaggtgcta
     2401 ttggtagaag acattgaaat gaatgtggtg gttgcccgag caatgttgga gaaaatcggt
     2461 tgcgtggttg atgtagcaat gagtggtgaa gaaacgttcc aacattttgc acagaacagc
     2521 tacgatctga ttttacttga tattcaactg ccggatacca ccggttttga aattgcacag
     2581 caattaagat tgcaatatga aaacgaggaa attgactatt taccgctgtt ggttgctttg
     2641 acggcaaatg tgatccaaag ccggcaggag taccaagcac aaggtatgga cgatgtgcta
     2701 cacaaaccgc tctctttaga tgaattaatg cgttgtctca accaacattt tggagatgat
     2761 tttttgcaaa aaaatgaaga aaaacagccg cttgtagaga gaggttctga gaatttacct
     2821 tatagcatta caattcttaa tgaattgatt ggggtaatgg ggaaagagaa tgtgcttgcc
     2881 aatatggatt tatttgctga tttaatgccc acttatttgg caaatttaca gcgttattta
     2941 agtgattggc aaaccacaaa ttctgtggaa agccgtaaat taacggcgga cgaggctcat
     3001 aagattaagg gagcattagc atcagtcgga ttacatcagc tacaacaaac tgctcagctt
     3061 gctcaatccg ataacggcga gcagtgggag caaaatattg cacactgggt ggaaatgatt
     3121 tgttccaaat ggcagatgga tttgcaatta gctaaggaat atgttagaag caagggggac
     3181 atttagtaaa tttgaaatgt tacgatggtg caagcgtcca cgcttgtacc aattttaata
     3241 tctgaagcac aagcgtggac gcttgtgcta tcaaaacgta tttaccattt cttggttttc
     3301 ggttttacaa attcaatgcc tttatcttta taggtaatat cttccagctg taatagaaaa
     3361 cgcttattcg gcaagccacc accgaatccg gttaaggctt gatttttccc taatacccta
     3421 tggcaaggca ctaaaatcga aatcgggttt cgcccaactg caccgccgac ggctcgcatc
     3481 gcacttggct tgcctagttg ggtggcaatt tcgccataag tagtagtttg cccgtaaggg
     3541 atttcacgca aaattttcca aactgattgc tggaatggag tgccttgcgg ggcaagaaaa
     3601 tccaactgct gaaaatccaa tggctcgccg gcaaaatagc ggtctaaaat ttctttggtt
     3661 ttgcaaaaaa tttctaaaat ctgaccactt gcagcttgag cggaaaggaa attatcaagc
     3721 gaggtggttt gttgctcttg ccctagctca atgtaaatca agccttcttc tgtggctaaa
     3781 agcaataatt gcccgatagg gcttgggtag taatcgtagt aaatttgaga tttcatgttt
     3841 ttatgccaat aaaaaaagag gtgtaattga ttttacacct ctttaaacct taattcatca
     3901 aattagaatt tgtagtttac gtttaaaccg taaagatttg cacgagcttt gctcttgaat
     3961 gtggctaaac cgtcttcaga gaaggtcagg ttgctaccac gaagatgtga gtaacctaca
     4021 tcagcagata ggtttggcgt gaaacgcacg gttgcaccta cagaatacca agcacgatca
     4081 gtgtccggaa ttgagattga tggatgagtt acacttgcac tttcatcaaa tgctacacct
     4141 gtacgcaatg ttagcatttc attcacatca taagacaagc ctaatgcaat acgttgagag
     4201 tcattaaagt tctcggtttt gtgcaatagg cggttacctt gcatatcata agcttctagg
     4261 ctttggaatt tgctccagtc tgtgcgttta tagctgtatt gtgcagcaag tttatcggtt
     4321 agtttgtggt aacctgacac ttcccagaat gccggtaggc gtaatcttag tgtaccatca
     4381 atctctgcac cgccggtaat ggtatctacc atcttaggaa taactacagg aacggtatta
     4441 gaatatttgc ctttaaattt aagatcaatt tcagagtggt aagtaaaacc taaacggttt
     4501 ctttcattga tattataaac caagccaaca ttccaaccca atgcccattt atcgccttta
     4561 agtttatgaa ttgttgaact tgcattggta ttttgaatgc cattaaccaa acgttctaat
     4621 tgagcagaat tcgtgatggt ttggtttaaa ttttgagatt ttaataaacc gtttacttga
     4681 gctaatactt gtgttttttg agcttcaaga gcggtattac ctgctgcgat acctgcctca
     4741 agagctttta aaccttgaat tgcactttgt acttgtactt tcttgtcttc caaaaattgt
     4801 ttagctggag caaaacgtgc cgcaccggca cctaagaaac gtcctaattc tgcttttgcg
     4861 tgtactgcat tcaaacctgc ccctaataca aaaccataac ctaaatcata agccgcacta
     4921 aaattaacat tcacagcact taatgatgta cgaccaccta agaaaccagc agaatagtta
     4981 gggtcaaatt cagatatcaa accataattt acatttacgc caccaccaat agaaaggcgc
     5041 tcattgatag gtgaaacgat ataagcattt ggaacaatag cggtaggaat gacattttta
     5101 tgtgaggcat ctgcacctgt agcattgagc tttccatcaa tatttacatc agcatctacg
     5161 aaaataccac cggcagaaat ttcggtttgt ttaaataacg tcattaacgc agggttggtt
     5221 gcaacaacag aagcgttatc ggctacagcg gcattacctg cgtaagctct acctaaaccg
     5281 gatgttgaaa tttcagctaa ttggaatgcg gcagcagatg caccggctga gctgaatgct
     5341 actaatgaag cgatt
//
LOCUS       DANLXF010000165         5336 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.165, whole genome shotgun sequence.
ACCESSION   DANLXF010000165 DANLXF010000000
VERSION     DANLXF010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.165"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..397
                     /locus_tag="PXD66_001926"
     CDS             <1..397
                     /locus_tag="PXD66_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HDL6320195.1"
                     /translation="GSYPAIKEIGEERAKKHQDILDLNRELYSAMVNRDFSALQTLLS
                     DDFELYSSTGEVIDKQEWIKLIQKRVLEFDKVDLLSTDFQGNQSSGLLRVNGEFFGIE
                     RDNVVVEMNIRTIVRNTERQIKCIVIKEV"
     gene            410..1303
                     /gene="murQ"
                     /locus_tag="PXD66_001927"
     CDS             410..1303
                     /gene="murQ"
                     /locus_tag="PXD66_001927"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDL6320196.1"
                     /translation="MNALGQMITEQRNPNSMNIDRLSALDIVQVINQEDKQVAIAVEQ
                     CLPQIAQAVEKIVQAFEKGGRLVYVGAGTSGRLGVLDASECPPTYGVKPEMVVGLIAG
                     GERALRFPIEGAEDNREQGKVDLQAVNFSEKDVLVGIAASGRTPYVLGAMEYAKQLGA
                     TTVSIASNPNSAMAQIADIAIETVVGPEVLTGSSRMKSGTAQKLVLNMLTTASMILIG
                     KCYQNLMVDVQATNEKLKARAIRIVMQATECEREVAEDTLKLAENNAKLAIMMILGNL
                     DKQKAEDLLSQNQGRLQKALK"
     gene            complement(1387..2331)
                     /gene="prmB"
                     /locus_tag="PXD66_001928"
     CDS             complement(1387..2331)
                     /gene="prmB"
                     /locus_tag="PXD66_001928"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDL6320197.1"
                     /translation="MFKYNTELLDEISALPIVNDLVTIQDMMRWGYSYFNASDLYYGH
                     GYDNAWDEAQQLVLAGLYLPFDVPETLYSSRLTRVEKERIINLICERLGSRKPVAYLT
                     NSAWFCGGEYYVDERVIVPRSPIGELIKKGFKGIIKNEPHRILDMCTGSGCIAIACAE
                     RFPEAEVDAVDLSLDALDVAQINIERHQVSHRVFPICSDLFNNLPQDKYDLIVTNPPY
                     VDEEDLGDMPEEFHHEPALALGSGKDGLDLTKRILAQAADYLNDDGVLVCEVGNSMVH
                     LIEQFPSVPFNWLEFEHGGLGVFSLTKEELVKHRHLFA"
     gene            2424..2969
                     /gene="smrB"
                     /locus_tag="PXD66_001929"
     CDS             2424..2969
                     /gene="smrB"
                     /locus_tag="PXD66_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HDL6320198.1"
                     /translation="MNDTLLDDEFALFREAAKGTKKLTQNTFVPKAEPRKKLQELREL
                     KEKADTEFYFSDEYEPLLKEENEKVKYLREDVDPYILKQLRRGDFQPELFLDLHGLTR
                     EKAKKELAALILACEREKVYCASVMTGFGTRTLKDQIPRWLVQHPKVMALHQAPKEWG
                     GDASILILVEQPENPEKMFGR"
     gene            3033..3518
                     /locus_tag="PXD66_001930"
     CDS             3033..3518
                     /locus_tag="PXD66_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDL6320199.1"
                     /translation="MSEQTFHQLTALLDSHRARYRVVGHPSAGKSEEVAKIRGTEIGQ
                     GAKALVCKVKGNGVKQAVLAILPADYQADLTKIAEHLGGLRASLASPAEVAELTDCVF
                     GAIPPFSFHPDLLLVAEPSLFERYAELAFNAGTLERSIILNTEDYQKIVNPVLVQFAK
                     S"
     gene            complement(3520..4269)
                     /locus_tag="PXD66_001931"
     CDS             complement(3520..4269)
                     /locus_tag="PXD66_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL6320200.1"
                     /translation="MEKRFEINEQGLSIRCKLFYEKELQQIENVVIVLHGFGSSKDLK
                     SNTKFGERLSSKYKGYAALAFDFPCHGDDARKKLSVEESITYLQLVIDYAKTSLNAKN
                     LYAYGTSFGGYLTLKYLAEKGNPFRKIALRAPAVQIYHSLMNRLSEEERQKLAKGREI
                     LWGFDRQMKISQAFFDELTDITQNDYLDYAEQILILHGTEDEMVALSTSQQFADNNVI
                     ELLPIEGADHAFSNPKLMDIAIGKIVEFFAP"
     gene            4441..5304
                     /locus_tag="PXD66_001932"
     CDS             4441..5304
                     /locus_tag="PXD66_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HDL6320201.1"
                     /translation="MLQLILAILCSVTVGVLIKFARQKGIRIDQSIAVNYIVTTTMTF
                     LLLKPDYQGQSITDIIANNPTSYIFVILSITLPTIFLVQAKALEFAGIIRTDAAQRLS
                     LFLPILAAFTIFGEQVTQNKLIALLLAFVALGCLLWKGNQGMEKGGKIAIISLALVWV
                     GYGVNDILFKQIAKSGSAFPVTLSIAFIGAGCFMFIYLLLKRTQWHVPSVLTGLLLGV
                     LNFGNILFYIQAHQAMKDDPTLVFTGMNLGVICLGTFVGAFFFKEKIHKVNYLGVAIA
                     IVAIICLFYWR"
BASE COUNT         1601 a         1026 c         1215 g         1494 t
ORIGIN      
        1 tggaagttat ccggcaataa aagagattgg cgaggagcgg gcaaaaaaac atcaagatat
       61 attagattta aatagagaac tttatagtgc aatggtgaac cgtgattttt cggctttgca
      121 aacattacta agcgatgatt ttgagcttta ttcatcaaca ggtgaagtga ttgataagca
      181 agaatggatc aaattaatcc aaaagagagt attggaattt gataaggttg atttgctatc
      241 taccgatttt caggggaatc aatcatctgg attattaaga gtaaatggtg agttttttgg
      301 gatagagcgg gataatgtgg tggtagaaat gaatattcga accattgtga gaaatacaga
      361 acgccaaatt aaatgtattg tgattaaaga ggtttaagtg tcgcaactat tgaatgcctt
      421 agggcagatg attaccgaac aacgtaatcc gaattcgatg aatattgacc gcctttcagc
      481 gttagatata gtccaagtca tcaaccaaga agacaaacaa gtagcgattg cggtggagca
      541 gtgcctgcct caaattgcac aagcggtgga aaaaattgtg caagcctttg agaaaggtgg
      601 acgattggtt tatgtcggtg ccggcacgag cggaagatta ggggtgcttg atgcttcgga
      661 atgtccgccc acttatggcg taaaacctga aatggtggtt ggattaattg ccggtggtga
      721 gcgggcatta cgtttcccga ttgaaggggc ggaagataat cgtgagcaag gcaaagtaga
      781 tttacaagcg gtcaatttta gcgaaaaaga tgtattggtc gggattgcag ccagtgggcg
      841 aacgccttat gtgttagggg cgatggaata tgctaagcaa cttggtgcga ccacggtgtc
      901 gattgccagt aacccaaatt cggcaatggc acaaattgcg gatattgcga ttgaaaccgt
      961 tgtcgggccg gaagtgctaa ccggttccag ccgtatgaaa tccggcacag ctcaaaaatt
     1021 ggtattgaat atgctcacta ctgcgtcgat gattttaatc ggtaaatgct accaaaattt
     1081 aatggtagat gtgcaagcaa ctaatgaaaa actcaaagct agagcaattc ggattgtgat
     1141 gcaagcgaca gaatgtgaac gagaagtggc tgaagataca ctaaaattag ccgaaaataa
     1201 cgctaaattg gcaattatga tgattttagg taacttagat aagcagaaag ccgaagattt
     1261 attaagccaa aatcaagggc gattacaaaa agcgttaaaa tagaagtatg tctgctctcc
     1321 ccttgtggga gagagacaga tcaatcattc attcagaatg attgatcaga gagaggggag
     1381 gcagatttaa gcaaataaat ggcgatgttt cactagttct tctttggtta aactgaacac
     1441 ccctaagcca ccgtgttcaa attccagcca gttaaacggc acactgggga attgttcgat
     1501 taaatgcacc atactgttgc cgacttcgca gactagcacg ccatcatcat ttaagtaatc
     1561 agcggcttga gcaagaatcc gcttggttaa atctaaccca tctttgcccg agccaagagc
     1621 taatgccggc tcgtgatgga actcttcggg catatcgcct aagtcttctt catcaacata
     1681 aggcggatta gtcacaatta agtcgtattt atcttgcggt aaattgttaa acagatcgga
     1741 acagatcggg aaaactcggt gagacacctg atgacgctca atattaattt gagcaacgtc
     1801 taaggcatca agggaaagat caaccgcatc gacttctgct tccggaaaac gctcggcaca
     1861 ggcaatcgca atacaaccgc tgccggtaca catatctaaa attcgatgtg gctcgttttt
     1921 gataatgcct ttaaaccctt ttttgatcag ctcaccaatc ggtgaacgtg gcacgatcac
     1981 acgctcatcg acataatatt caccgccaca aaaccaagcc gagttggtaa ggtatgctac
     2041 aggtttgcga gagcctaaac gctcacagat taaattgatg atacgttctt tctcaactcg
     2101 agttaagcga gaggaataaa gtgtttccgg tacatcaaac ggcaaataaa gccctgccaa
     2161 cactaattgt tgtgcctcgt cccacgcatt atcgtagccg tgcccgtaat aaagatcaga
     2221 ggcattaaaa tagctatacc cccaacgcat catatcttga atagtgacta aatcattcac
     2281 aatgggcagg gcggaaattt catcaagaag ttcggtatta tatttaaaca ttggcgtgcc
     2341 ttttttcgta ttgttaaaat ggctgtgtta taccacattt tgctttgtgt tagaatgtaa
     2401 aaatgaataa gaaggaaaag ataatgaacg atacgttatt agacgatgaa tttgcattat
     2461 tcagagaggc agcaaaaggc acgaaaaagc tgacacaaaa tacctttgtg ccgaaagcgg
     2521 aacctcgcaa gaaattgcaa gaattgcgag aattaaaaga aaaagctgat acagaatttt
     2581 atttctcgga tgaatatgag cctttgctga aagaagaaaa tgaaaaagtg aaatatctgc
     2641 gagaagatgt tgatccttat attctcaaac agttacggcg aggcgatttc caacccgagc
     2701 tgtttttaga tctgcatggt ttaactcgag aaaaagcgaa gaaagagttg gcagcgttga
     2761 ttttagcctg tgagcgggaa aaggtatatt gtgccagtgt gatgaccggc tttggcactc
     2821 gcacgctcaa agatcaaatt ccacgctggt tggttcagca cccaaaagta atggcgttgc
     2881 accaggcccc gaaagagtgg ggcggagatg cctctattct aattttggtt gagcagcctg
     2941 aaaatcctga aaaaatgttt gggcgttaaa attcggctga attgccaatt ttttattaaa
     3001 aacgaccgct tgtgtatcat taaggagaaa tgatgtcaga acaaactttc caccaactta
     3061 cagcattatt agattcgcat cgagctcgct atcgagtggt aggacatcct tctgccggga
     3121 aatcggaaga agtggcaaaa attcgaggaa cagaaatcgg gcaaggggcg aaagccttag
     3181 tttgcaaggt aaaaggcaat ggggtaaagc aagctgtgct tgctatatta cctgctgatt
     3241 accaagcgga tttaacaaaa attgcagagc atcttggtgg gttaagagcc tcacttgcca
     3301 gcccggctga agtggcagaa ttgaccgatt gtgtatttgg ggcaattccg ccgtttagct
     3361 ttcaccccga tttattattg gtggctgagc caagtctgtt tgagcgttat gctgaattag
     3421 cctttaatgc cggtacgttg gaacgttcga ttattctgaa taccgaggat taccaaaaga
     3481 tagttaatcc tgtgttggtt caattcgcca aaagctaggt tatggggcga aaaactccac
     3541 gattttgcca atagcgatat ccattaattt agggttagaa aaagcgtgat ctgccccttc
     3601 aatcgggagc agttcaatca cgttattatc ggcaaactgc tgtgaagtgc ttaacgccac
     3661 catttcatct tccgtgccgt gcagaattaa aatttgctcg gcataatcca aataatcgtt
     3721 ttgggtaata tcggttagct catcaaaaaa ggcttggctg attttcatct ggcgatcaaa
     3781 cccccacaaa atttctcgcc ctttggcaag tttttgccgt tcctcttctg acaagcggtt
     3841 cattaacgaa tggtagattt gtactgccgg tgcccgcaat gcaattttcc gaaacggatt
     3901 gcctttttcc gccagatatt tcaaggttaa atagccgcca aaactagtgc cgtaggcata
     3961 cagattttta gcattcaggc tggttttggc ataatcaatc accagttgca gataagtaat
     4021 ggattcctca accgagagtt ttttacgggc atcatcaccg tggcaaggga aatcaaaggc
     4081 aagggcagcg tagcctttat attttgagct aaggcgttcc ccaaatttgg tattcgattt
     4141 cagatcttta gacgaaccaa agccgtgcaa cacaatcact acattctcga tctgctgcaa
     4201 ttctttttca taaaacagtt tacagcgaat gctgagaccc tgttcattaa tctcaaagcg
     4261 tttttccatt ttatgttaag tattgaaatt gaagatgaca aaattttagc actatttgag
     4321 tgcaagcggt cggatctctc gcaaaatttg caaattttcc ggtaaattta accgcttgta
     4381 ctagccaaaa tttaagcata tttatagaat acagccttcg tttttaaaag gagaaatgaa
     4441 atgttacaac ttattttagc gattttatgt agcgtgactg taggcgtgtt gattaaattc
     4501 gcccgccaaa aagggattcg tatcgaccaa agtattgcgg tgaactatat cgtgactacc
     4561 acgatgacgt ttttattact aaaaccggat taccaagggc agtcgattac cgatattatt
     4621 gctaataacc cgacttccta tatttttgtg attttaagta tcacattgcc tactattttc
     4681 ttggtgcaag caaaagccct agagtttgcc ggtattatcc gaaccgatgc ggcacaacgt
     4741 ttatcgctgt tcctgccgat tttggcggca ttcaccattt ttggtgagca ggttacgcaa
     4801 aataaattaa ttgcacttct gttagctttc gtggcactcg gttgcttatt atggaaaggc
     4861 aatcagggaa tggaaaaagg cggaaaaatt gcgattatca gccttgctct ggtttgggta
     4921 ggctatggcg tgaacgatat tttatttaaa cagattgcca aatcaggctc tgctttccct
     4981 gtcacccttt cgattgcctt tatcggtgca ggctgtttta tgtttatcta tttgctactt
     5041 aaacgcactc aatggcacgt tccaagtgtc ttaaccggct tattgctagg cgtgttgaac
     5101 ttcggcaata ttctgttcta tattcaagct caccaagcca tgaaagatga cccaacttta
     5161 gtcttcactg gaatgaactt aggggtaatt tgcttaggca ctttcgttgg agcattcttc
     5221 tttaaagaga aaatccacaa agtgaactac ttaggcgtgg cgattgcgat tgtggcgatt
     5281 atttgcttgt tttattggcg gtaaatatac tttccaaaat taagggcgaa cataat
//
LOCUS       DANLXF010000166         5278 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.166, whole genome shotgun sequence.
ACCESSION   DANLXF010000166 DANLXF010000000
VERSION     DANLXF010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5278
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.166"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(115..957)
                     /locus_tag="PXD66_001933"
     CDS             complement(115..957)
                     /locus_tag="PXD66_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HDL6320202.1"
                     /translation="MKVGLVLEGGGLRGIFTAGVLDVFLDEHIHIDGAIGVSAGALFG
                     VNLPSKQKGRALRYNKKYAKDKRYMGLHSLLTTGNIVNRDFAYYELPSKLDPFDQQAF
                     EDSGMDFWVTVTNVETGEPEYIKIDNVFEQMEAFRATSSMPIVSKIVEIEGKKYLDGG
                     VSDSIPVQKFIEMGYDKIIVVLTRPLEYRKKPSSMWLFKRFYKKYPKLVQRWENRYAE
                     YNQAVEQIIQLNEKQQIFVIRPSRTVKISRLETDVNKIQAMYDLGVEDAKNALAGLRA
                     YLAK"
     gene            1017..1334
                     /locus_tag="PXD66_001934"
     CDS             1017..1334
                     /locus_tag="PXD66_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HDL6320203.1"
                     /translation="MADAEFMDFLLDQLSPISGLRSKKMFGEYCLFFDNKIVAIISKD
                     YRIFVKANPQTLPLFLAENAEQFHYFAKGKVQKMHYWSIPELAIEEADELVKWIRLGL
                     QAV"
     gene            1425..2138
                     /locus_tag="PXD66_001935"
     CDS             1425..2138
                     /locus_tag="PXD66_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease"
                     /protein_id="HDL6320204.1"
                     /translation="MNQKKLVSLIVVIVLAIISQIFSFFDKKEPSKAQEKSTQSSQSH
                     QVSTSSLGNYDVQMKNDRLKQHIVDTDYYTLALSWSPAFCQKQKRNNHGKIPSHLEYQ
                     CGGSADFGWVIHGLWPQSGKARSVDEHPRYCQGDLPMVAEQTIKQYLPESPGATLLQG
                     EWEKHGACAFNQADDYFAKQKELFNQLKLPDTEMSKLALFNWMRANNPHLQNVYLGGS
                     KDELYICYDKAFQPMDCPR"
     gene            2138..2632
                     /locus_tag="PXD66_001936"
     CDS             2138..2632
                     /locus_tag="PXD66_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HDL6320205.1"
                     /translation="MADLRQLKMQVQRQLKRDLERANHYFNTTFTPPSISYAVRGVKA
                     GVAYLERNEVRFNPVLLQENEQAFIRQVVPHELAHILVYQHFGRVQSHGKEWKMMMET
                     VLGVPAEIYHCFDTQSVQQQFSYQCACQTHQLSIRRHNAVMRDKKSYICRLCKTQLHF
                     VEKS"
     gene            2737..4017
                     /locus_tag="PXD66_001937"
     CDS             2737..4017
                     /locus_tag="PXD66_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL6320206.1"
                     /translation="METIVIVGGGAGGLELATYLGNKLGRKQKAKVVLIDRNATHLWK
                     PLLHEVATGTIDEGTDALSYRAHAKNHSFEFQQGTLVAVDREKKTVSLAPIYNDQNQL
                     LVKERHISYDKLVIAIGSKSNDFGTKGVAENCIFLDGSEQAKDFQKRMMELFLRFSHS
                     ENKDVKIAIVGGGATGIELSAELYHVVKNLNSYGFGKLNRASLKVTLIEAGPRLIPAL
                     PEKVSVSAFHELKKAGVSVRLGTMITEARPDGLMTRLGEKIEADIMVWAAGVKAPEIT
                     REFGFETNRLNQIEIKDTLQTTVDESVFVIGDCASLIQDGKPIPPRAQAAHQMATRCG
                     KNIVALIEGKEMKPFRFNDKGSLLSFSKFGTVGNLMGNLVSGDMFIEGKIARLAYLSL
                     YRMHQVALHGCVKTGLIVLVGQINRFLRPTMKLH"
     gene            complement(4104..5141)
                     /locus_tag="PXD66_001938"
     CDS             complement(4104..5141)
                     /locus_tag="PXD66_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL6320207.1"
                     /translation="MNVLKLISLKSILIASGIFLSAQAAQAEGRLTVYCSVQNNVCEE
                     MTKQFSHKYNVETKFIHGGTGTIFGKVKAEASNPQADIWYGGTIEPHFQAGELGLLEA
                     YRSPKQAEILPQFKSLVESEQGKFTSIVYMLVLGLGVNTEKLAKLGIEAPKKWEDLSD
                     PRLKGEVQLPDPRSSGTTYTFMTTLSTLWGEEKTFDYLKKLNNNVSQYVKSTLVTSNL
                     ARGESAVTVGFVHSYATEKEKGAPIEAIIPEGKVGYALGGVSIIKNARNLDNAKLFMD
                     WVLSKEAQEIPWKKHGVYQTPTNINAEVAPQSTKAEALDFVEIDYQKAGSSEEGKRLI
                     DKWLAEIKLAN"
BASE COUNT         1535 a         1025 c         1116 g         1602 t
ORIGIN      
        1 tatataaaat gtttggacag gcacaggacc ggtccctata aaatttgttt ggaagtgttt
       61 ccgtctagac gtctataata tattttttga acgttttagg caaatagaat ttgtttattt
      121 ggctaaatat gcccttaaac cagccaacgc atttttggca tcttctaccc ctaaatcgta
      181 cattgcttgt attttattta catccgtttc aaggcgactg attttgactg ttctgcttgg
      241 acgaatcaca aaaatctgtt gtttttcatt gagttgaata atctgctcaa cggcttgatt
      301 atattccgca tagcggtttt cccagcgttg cactaatttc ggatattttt tataaaaacg
      361 tttaaacagc cacatcgagc ttggtttctt acggtattca agtgggcgag taagcaccac
      421 gataattttg tcatatccca tttcaataaa tttctgcacc ggaatactgt ctgacacacc
      481 accgtctaaa tattttttac cctcaatttc gactattttg gatacaatcg gcattgagga
      541 agtagctcga aaggcttcca tttgctcgaa cacattgtca attttgatgt attcgggctc
      601 accggtttcg acattggtta cagtaaccca aaagtccata ccggaatcct caaaggcttg
      661 ttggtcaaac ggatccagtt tggaagggag ttcatagtaa gcaaaatccc gattaacgat
      721 attgccggtg gtaagcaagc tgtgtaaccc catataccgc ttatctttag cgtatttttt
      781 gttgtatcgc aacgccctac ctttttgttt tgaaggcaaa ttcacgccaa ataacgcacc
      841 ggctgaaaca ccgattgctc catctatatg aatatgttca tctaaaaata catctaacac
      901 gccggcagta aaaatacctc gcaaaccacc gccttccagc actaatccaa ctttcatatc
      961 attctccctg taatttgtgt attataaccc gaattatctt gctaggagaa atcacaatgg
     1021 ctgatgcaga atttatggat ttcctgttag atcaactaag cccgatttcc ggtttgcgtt
     1081 cgaaaaaaat gtttggtgaa tattgcttgt tttttgataa taaaattgtc gctattatca
     1141 gcaaagatta tcgcattttt gtcaaagcta atccgcaaac cttgccgtta ttcttagctg
     1201 aaaatgccga acaatttcac tattttgcta aaggaaaagt gcaaaaaatg cactactgga
     1261 gcattccgga attagcgatt gaagaagccg atgaactggt caaatggatt cggctaggct
     1321 tacaagcggt gtaatttagc gaaaattttg taaaaaaacg ctaaaatatg cccgcttgtt
     1381 aggggggggg acctaatatt tctatacttt aagattttat atttatgaac caaaaaaaat
     1441 tagtttcttt gattgtcgtt attgtgttgg caatcatttc tcaaattttt agcttttttg
     1501 ataaaaaaga gccttcaaaa gcacaagaaa aatcgaccca gagtagtcag tctcatcaag
     1561 tttcaacttc atcactaggg aactatgatg tgcagatgaa aaacgaccgt ttgaaacagc
     1621 atattgtaga taccgattat tacaccttag cgttgtcgtg gtcgccggcg ttttgccaaa
     1681 aacaaaagcg aaataatcac ggtaaaattc cgtctcattt ggaatatcaa tgtggtggct
     1741 cagcggattt tggttgggtt attcacggct tatggccgca aagtggcaag gcaagaagtg
     1801 ttgatgaaca ccctcgttat tgccaaggcg atttgccgat ggtggcagag cagacgatta
     1861 agcaatattt acctgaatcg ccgggggcaa ctttgttgca aggcgaatgg gaaaaacacg
     1921 gtgcttgtgc ctttaaccaa gcggatgatt acttcgccaa acaaaaagag ctatttaatc
     1981 agctcaaatt accggatacc gaaatgtcta aattagcact gtttaactgg atgcgagcca
     2041 ataaccctca tttgcaaaat gtctatttag ggggtagcaa agatgagctg tatatttgct
     2101 atgataaagc atttcaacca atggattgcc cacgctaatg gcagatcttc gccaactgaa
     2161 aatgcaggtt cagcgccaat taaagcgtga tcttgaacgt gcaaatcact attttaatac
     2221 cacttttacg ccaccaagta ttagctatgc ggtgcgtggc gtaaaagccg gtgtggctta
     2281 tcttgaacgc aacgaagtac ggtttaatcc tgtgttattg caagaaaatg aacaggcatt
     2341 tatccgccaa gttgtgccgc acgaactggc tcatatcttg gtttatcaac attttgggcg
     2401 ggtacaatct catggtaaag agtggaaaat gatgatggaa accgtactgg gtgtgccggc
     2461 ggaaatttac cattgttttg atacccaaag tgtgcaacag caatttagtt accaatgtgc
     2521 ctgccagact caccagcttt cgattcgccg acacaatgcc gtgatgcgag ataaaaaaag
     2581 ctatatttgc cgtttatgta aaactcaact ccattttgta gaaaaaagtt aatgtggaga
     2641 aaagttaaga gaatgttacg atttagctag acattcctgt ttaaacgcct acaatttgcc
     2701 gtagttttta tttttaattt tatttccgga gtatttatgg aaacaattgt aattgtaggt
     2761 ggcggtgcag gcggtttgga gcttgcaacc tatctaggca acaaattagg gcgtaaacaa
     2821 aaggcaaaag tggtgctgat tgaccgcaat gcaacgcatt tatggaaacc actgttgcac
     2881 gaggtggcaa ccggtactat tgatgaggga actgatgcgt taagctatcg ggctcacgct
     2941 aaaaatcata gttttgaatt tcagcaaggt acgctggtgg cggtagatag agagaagaaa
     3001 acggtttcac tcgccccgat ttataatgat caaaaccaac ttctcgttaa agaacgccat
     3061 atcagttatg acaaattagt gatcgcgatc ggcagtaaat ccaatgattt tggcaccaaa
     3121 ggggttgcag aaaattgcat tttcttagat ggctcagagc aagcaaaaga tttccaaaaa
     3181 cgaatgatgg agctgttttt acgtttttca catagtgaaa ataaagatgt aaaaattgcg
     3241 attgtggggg ggggagcaac aggtattgag ctttcagcgg agctttacca cgtggtgaaa
     3301 aatttaaatt cttacggctt tggtaaatta aaccgtgcca gcttgaaagt cactttaatt
     3361 gaggcaggcc ctcgtttgat tccggcatta ccggagaaag tgtcggtttc tgctttccac
     3421 gaattgaaaa aagcgggtgt ttcggtgcgt ttaggtacga tgattaccga agctcgtcca
     3481 gatgggctaa tgacccgttt aggtgagaaa attgaggcgg atattatggt ttgggcggct
     3541 ggcgtgaaag cccctgaaat tacccgtgaa tttggttttg aaactaaccg cttgaaccaa
     3601 attgagatta aagacacgct acaaaccacc gttgatgagt cagtgtttgt cattggcgat
     3661 tgtgcatcat taattcaaga tggcaagcca attccaccca gagcacaagc agcccaccaa
     3721 atggcaaccc gatgtggtaa aaatattgtg gcattgatag aaggaaaaga gatgaagccg
     3781 tttcgattta acgataaagg ctcactgctt tccttctcaa aattcggcac ggtgggtaat
     3841 ttaatgggga atttagtgag cggtgatatg tttattgaag gtaaaattgc ccgcttggct
     3901 tacctctcgc tttatcgaat gcaccaagtg gcattacacg gctgtgtcaa aaccggcttg
     3961 attgtgttgg tagggcaaat taaccgtttc ttaagaccaa caatgaaact acattaaaca
     4021 aaaatccgca tcaaatgatg cggatttttt atggcaaaat aacaagcggt tattttcttg
     4081 cctatttttg caaatactca cgcctagttt gctaatttaa tttccgccaa ccatttatca
     4141 attaagcgtt tgccctcttc gctggaacct gctttttggt aatcaatttc cacaaagtct
     4201 aacgcttcgg ctttggttga ttgtggagca acttcggcgt tgatgtttgt tggggtttgg
     4261 taaacaccgt gttttttcca aggaatttcc tgtgcttctt ttgaaagcac ccaatccata
     4321 aagagtttgg cgttatctaa attacgggca tttttgataa tgctgacacc gcctaacgca
     4381 tagcccactt taccttccgg aataatggct tcaataggcg cacctttttc tttttcagtg
     4441 gcataactat gtacaaagcc taccgtaacc gcactttcac cacgagcaag attgctggtg
     4501 actagggtgc ttttcacata ttgcgaaacg ttattgttga gttttttcag ataatcaaag
     4561 gttttctctt cgccccataa ggttgaaaga gtggtcataa acgtataagt cgtgccggag
     4621 ctacgaggat caggcagttg tacttcacct tttaagcgag gatctgataa atcttcccat
     4681 tttttcggtg cctcaatgcc tagtttggcg agtttttcgg tattcacgcc taaccctaat
     4741 acaagcatat aaacgataga ggtaaattta ccttgttcac tttcaactaa ggatttaaat
     4801 tgtggcagaa tttcagcttg ttttggtgaa cggtaggctt ccagtaagcc taattcgcct
     4861 gcttgaaagt gaggttcaat cgtgccgcca taccaaatat ctgcctgagg gttcgatgct
     4921 tccgctttta cttttccgaa aattgtaccg gttccgccgt gaatgaattt agtttcgaca
     4981 ttatatttgt gtgaaaattg cttggtcatt tcttcacaaa cattattttg taccgagcaa
     5041 taaacagtta aacgaccttc tgcttgtgct gcttgagctg ataagaaaat gccagaggcg
     5101 attaagatcg attttaatga gattaatttg agtacgttca tcactttcct gaaaacataa
     5161 aataaaaggt agtgaagtct agcatttcaa agcgctggtt gaaaatagtt aattgcaatg
     5221 aagtagggca attgtgccta ttactttaat atttaccaac tatttgcaag ttttagca
//
LOCUS       DANLXF010000167         5258 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.167, whole genome shotgun sequence.
ACCESSION   DANLXF010000167 DANLXF010000000
VERSION     DANLXF010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5258
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.167"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..326)
                     /locus_tag="PXD66_001939"
     CDS             complement(<1..326)
                     /locus_tag="PXD66_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320208.1"
                     /translation="MTLETFNQQKAKFHEQEKAVLAIQTELDKAKNILEALQNEKAEF
                     VARQKTNLANIGTLSADEYVEIKNKNSGLQARIEYYQALIVDLENKCYAEQEILFQQQ
                     NELKQI"
     gene            complement(336..542)
                     /locus_tag="PXD66_001940"
     CDS             complement(336..542)
                     /locus_tag="PXD66_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDL6320209.1"
                     /translation="MENTPAKQRFMKIDEVSAVTTLSETQIYRKMKAGDFPQSISIGA
                     NSKVWLESEIQQWIQRKIEQHRNQ"
     gene            complement(1012..2226)
                     /locus_tag="PXD66_001941"
     CDS             complement(1012..2226)
                     /locus_tag="PXD66_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL6320210.1"
                     /translation="MTATKKPLSDTLLKNLKYSPNAKPIADSNGLFILAQKKAKVWIY
                     SYISPKTGKRSTKNRIGSYPQMSLAEARLKRDELNTLVLQGLDPFEHLAKMQREAKEK
                     QESIQSFAYKWADWKLSKGKCKPKTMEKCLQRLENHLFPRFEGYTLEDFTLTDTINRL
                     TDLDRIMPDTLHRIAGNLIEILDYAVLCGRINFNPISVIKKAFSSVSTTHQPAIMPEE
                     LAEFMQDLQKSNRTPQIKLLVEWQLLNILRPFEAVAVQWSDIDWETKTLNIPADRMKG
                     GKNPHSVPLTKQAIAILEEMKKYNGHHKHIFTHRTDRNKPANSQTVNNAIKRLSGGKY
                     KGLLVAHGLRSIASTYLHERFTTETLVIEACLSHTNKDTVMSAYHRKNWLDRRRGIMQ
                     EWADFVEQCKSI"
     gene            complement(2429..2504)
                     /locus_tag="PXD66_001942"
     tRNA            complement(2429..2504)
                     /locus_tag="PXD66_001942"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2469..2471),aa:Ile,seq:cat)
     gene            complement(2598..3335)
                     /gene="pgeF"
                     /locus_tag="PXD66_001943"
     CDS             complement(2598..3335)
                     /gene="pgeF"
                     /locus_tag="PXD66_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HDL6320211.1"
                     /translation="MDKIIPNWNVPKHIHALTTLRTGGVSKPPFDSFNLGDHVNDEPQ
                     DIAQNRALLVEKFQLPQAPLFLTQTHSTRVIELPYSGDNIKADAIYTNQPNQVCLVMT
                     ADCLPVLFASQDGSEIAAAHAGWRGLCDGILEATVEKFNCPPHEISAWLGPAIGPNAF
                     QVGSEVADQFCAFDPRAKEALIEDSTTSGKFLGNLYQIATQRLNKLGITAISGGEYCT
                     YSQPELFFSYRRDKQTGRMATLIWRTE"
     gene            complement(3367..3753)
                     /locus_tag="PXD66_001944"
     CDS             complement(3367..3753)
                     /locus_tag="PXD66_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDL6320212.1"
                     /translation="MTVIHTENAPAAIGPYVQAVDLGNMVLTSGQIPVNPETGEIPSD
                     IVQQTRQSLNNVKAIIEQAGLTVADIVKTTVFVKDLNDFAKVNAEYQAFFQENEHPNF
                     PARSCVEVARLPKDVGIEIEAIAVRR"
     gene            3834..4835
                     /gene="yejK"
                     /locus_tag="PXD66_001945"
     CDS             3834..4835
                     /gene="yejK"
                     /locus_tag="PXD66_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HDL6320213.1"
                     /translation="MSINVSEIVLHQLQKTEGENPELNTFLRENLLAISPEVDQMMLQ
                     LHQAYQSKAKAYGIFKPESVFAQQLNRLLEQETDFLPFSHSCAKMLAAELIKYPFADG
                     GTFILCRYTFLATEYLFIALIDSRNSMLVDEQLEIKRTQYLEIAQYDIACRINLTELK
                     LDATSNRYLTFVKGRVGRKIADFFMDFLSAEEGFNPQLQNQTLLQAVSDYCEQGELSA
                     PQTREVKKQVFEYCKGQINSGEEIALNELSNALPTLNEIDFAQFTQEQEYGLEENIPP
                     MRNALKTLTKFSGSGKGVTISFNAELLGERLIWDELNDTLTIKGLPANLRDQLERNK"
     gene            4902..5243
                     /gene="bamE"
                     /locus_tag="PXD66_001946"
     CDS             4902..5243
                     /gene="bamE"
                     /locus_tag="PXD66_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HDL6320214.1"
                     /translation="MKMKSLLAAMLLALGVTACSTVEKVVYRIDVPQGNYLEQDKIAQ
                     LKVGMTAEQVEYLLGTPMLKNIFETHRWDYVFIKREGYKDPEQRNLFIYFDAKGLVSK
                     IDDSNANAEQK"
BASE COUNT         1417 a          970 c         1129 g         1742 t
ORIGIN      
        1 ctgatttgtt ttagttcatt ctgctgttgg aataatattt cttgctctgc gtagcattta
       61 ttttctaaat caacaatcaa cgcttgataa tattctatgc gtgcttgtaa accactattt
      121 ttatttttga tttcaacata ttcatctgct gaaagcgtgc cgatattggc taaattcgtt
      181 ttttgccgtg cgacaaattc cgctttttcg ttttgcaatg cctcaagaat atttttcgct
      241 ttgtctaatt cggtctgaat agctaaaacg gctttttctt gttcgtggaa tttagctttt
      301 tgttggttaa atgtttcaag tgtcataatt tgcccttatt ggttgcggtg ttgttcaatt
      361 ttgcgttgaa tccattgctg gatttcactt tctagccata ctttgctatt tgccccaata
      421 ctgatacttt ggggaaaatc gccggctttc attttgcgat aaatctgcgt ttctgaaagc
      481 gtagttacgg ctgaaacttc atctattttc ataaatcgct gttttgctgg tgtgttctcc
      541 attttgtttt actccgtaaa aatttgcttt ggcgtgctat tgcctgctaa cgtgtgaaag
      601 aataaatcat cttggatttt ttgctatttg ttagggctgt gaaaggagat tttacagatt
      661 agaggggctt aattctgatt tggaaaactt gcaagcggtt atttttctct gattttttgc
      721 aagttttcag cggttctttc atcgttctgt catcgagttt ttaaaagtgg tgtttctgcc
      781 tgcgtaaatt tgcgttttct tgcgtaaaat tgcgtaataa aaaacggcta tatgaaagtt
      841 tttcacgcta tacggggcaa tgtttgggct taattttgca tttgcgtaga aatcgagact
      901 ttaagtgtgc gggcgaggcg tgggatagac ggcacgggct gggagctgat aggaaaaaat
      961 gaaaattttt catcaagcaa aaagaaaggc ggattgctcc gcctttgtca tttaaatgct
     1021 cttgcattgt tctacaaaat ccgcccattc ctgcattatt ccacgtctgc ggtctagcca
     1081 gtttttacgg tggtatgcac tcattacggt gtctttgttc gtgtgggata aacaagcctc
     1141 aattactagc gtttcagtag taaaacgttc gtgtaaatag gtgctggcaa tgcttcttaa
     1201 accgtgagcc actaaaagcc ctttatattt tccaccgctt aagcgtttta ttgcattatt
     1261 cacggtttgg ctattagccg gtttgtttcg gtctgttcgg tgtgtaaaaa tatgtttatg
     1321 gtgtccgtta tatttcttca tttcctctag gattgcgata gcttgttttg ttagcggtac
     1381 agaatgcgga ttttttccgc ctttcattct atctgccgga atgttgagcg tcttagtttc
     1441 ccaatctata tcgctccatt gcaccgctac ggcttcaaac ggtctaagaa tattcaagag
     1501 ttgccattca actaataatt taatttgagg cgttctattt gatttctgca agtcttgcat
     1561 aaattcggct aactcttcgg gcattattgc tggctggtgt gtcgtgctta cgctggaaaa
     1621 cgccttttta atgacgctga ttgggttaaa attaattctc ccacacaata ccgcataatc
     1681 aagaatttct attaaattcc ctgctattct gtgcaaagtg tcgggcatta tccgatctaa
     1741 gtccgttaag cggttgattg tatcggttaa ggtaaaatct tccagcgtat agccttcaaa
     1801 acgtgggaat aaatgatttt ctaaccgctg taagcatttt tccattgttt taggcttgca
     1861 ttttcccttt gatagcttcc aatcagccca tttataggcg aaagattgaa tagattcttg
     1921 cttttcctta gcttctcttt gcattttggc taaatgctcg aaagggtcta agccttgtaa
     1981 aacaagggta tttagttcgt ctctctttaa gcgtgcttcg gctaagctca tttgcggata
     2041 gctaccgatt ctattttttg tgcttcgctt gcctgttttg gggctgatat agctataaat
     2101 ccatactttc gcttttttct gtgcaagaat aaatagcccg ttgctgtctg cgataggctt
     2161 cgcatttggg gaatatttga gattttttag taatgtgtct gaaagtggtt ttttagttgc
     2221 agtcatttct tctaatcctt gatatataag ggtttatcga agtttatgat ccctaaattt
     2281 tcccgttttc aaaataggga tcattttagg gatcataaac actaagatac aatgcaacta
     2341 aagaagacaa taagagacta taaaagaatc taagttcttg attttatagt aaatttgaga
     2401 caatgaaaga caatatgcaa cgcttagatg gtccccccag ccggacttga accagcgacc
     2461 aagcgattat gagtcgcctg ctctaaccac tgagctatgg ggggatttga aaagtggttg
     2521 gaattataga gaaaaaccga gtagtcgtct agaggcagaa gattactcgg ttaattattg
     2581 aacgagagaa aatagggtta ttctgttcgc caaattaagg ttgccattcg cccggtttgt
     2641 ttgtctcggc ggtaggaaaa aaacagttct ggttggctgt aggtgcaata ttcaccgcct
     2701 gaaatggcgg taatgcctaa tttattcaga cgttgggtgg caatttggta aagatttcct
     2761 aaaaatttac cgcttgtagt gctgtcttca ataagggctt ctttcgctct tgggtcaaaa
     2821 gcacagaatt ggtctgccac ttcactcccg acttgaaaag cattcggtcc gatagccggc
     2881 cctagccaag ctgatatttc gtgtggcggg cagttgaatt tctctaccgt cgcctctaat
     2941 atgccatcgc ataacccacg ccagccggcg tgtgccgccg caatttccga cccgtcttgg
     3001 ctggcaaata ataccggcaa acaatctgca gtcatcacta agcagacttg gtttggttga
     3061 ttggtgtaaa tcgcatcggc tttaatatta tcacctgaat agggaagttc aatcactcgg
     3121 gtgctgtgag tttgggttaa aaacagcgga gcttgtggta attgaaattt ctcaaccaat
     3181 aaagcacgat tttgagcgat atcttgtggc tcgtcattaa cgtgatcacc taagttgaaa
     3241 ctgtcaaaag gcggtttgct tactccgcct gttcttaagg tcgtcagtgc gtgaatatgt
     3301 ttaggcacat tccaatttgg aataatttta tccatttgca aaaaatctca agaaaatgac
     3361 cgcttgttat cggcgtactg caatcgcttc gatctcaatg ccaacatctt ttggtaaacg
     3421 agccacttct acgcaagaac gagccggaaa attagggtgt tcgttttctt ggaagaaggc
     3481 ttggtattcc gcatttacct ttgcgaagtc gttaagatct ttgacaaata cggtggtctt
     3541 tacaatatcg gcaacggtta agccggcttg ttcgataatg gctttcacgt tgttcagaga
     3601 ttggcgggtt tgttgcacaa tatcactcgg gatttcgccg gtttcaggat tcacgggaat
     3661 ttgcccagaa gttaaaacca tattgcctaa atcaactgct tgcacataag gcccaatcgc
     3721 tgccggtgca ttttctgtgt ggataactgt catcttgtgt tcctcttaat gaaaaataaa
     3781 cggattaatg ataatatctt ttcttattat ttggaataaa gagaagtgaa ttaatgagca
     3841 ttaatgtttc tgaaatcgtg ttacatcaat tacaaaaaac agagggcgag aacccggagc
     3901 ttaatacgtt tttgcgagaa aatttattgg caatttcccc tgaagtcgat caaatgatgt
     3961 tgcaattaca tcaagcctat caaagtaaag caaaggctta cggtatattt aaaccggagt
     4021 cggttttcgc tcaacagctc aaccgcttgt tggagcaaga aaccgatttt ttacctttta
     4081 gccatagctg tgccaaaatg cttgcagcag agctgattaa atatccgttt gccgatggtg
     4141 gcacttttat tttgtgtcgt tacacttttt tagctaccga atatttgttt attgcgttga
     4201 ttgatagccg taattcaatg ctggttgatg agcagttaga gattaaacgc acgcaatatc
     4261 tcgaaattgc acaatatgat attgcctgcc gcattaattt aactgaactg aaacttgacg
     4321 caacgtcaaa tcgctattta acttttgtga aaggcagagt agggcgtaaa attgccgatt
     4381 tctttatgga ttttttaagt gcggaagaag ggtttaatcc acaattgcaa aatcaaacct
     4441 tgttgcaagc ggtgagcgat tattgtgagc aaggggagtt atctgcccct caaactcgag
     4501 aagtgaaaaa gcaggtgttt gaatattgta aagggcagat aaatagtgga gaggaaattg
     4561 cgttaaatga gctttccaac gcattgccaa cactgaatga aatcgatttt gcccaattta
     4621 cgcaagagca agaatacggc ttggaagaga atattccgcc tatgcgaaat gcccttaaaa
     4681 ccctgacgaa attttccggt tcgggcaaag gagtaacgat tagctttaac gcagaattat
     4741 taggagagcg tttaatttgg gatgaactaa acgacacact gacaatcaaa ggattaccgg
     4801 caaatctgcg tgaccagctg gaacgtaaca aataatggaa aatttttatc agtttaggta
     4861 ttatctcgtt atctgaattt tggattatca aaaggtcaat tatgaaaatg aaatctcttt
     4921 tagctgcaat gttgcttgca ctgggtgtaa cggcttgttc aaccgttgag aaagtggtgt
     4981 accgtattga tgtaccgcaa ggcaattact tagaacaaga taaaattgct caacttaaag
     5041 tcggtatgac agcggaacaa gttgaatatt tgcttggtac accaatgtta aagaacattt
     5101 ttgaaaccca ccgttgggat tatgtgttta ttaaacgtga aggttataaa gatcctgaac
     5161 aacgcaatct gtttatttat tttgatgcta aagggttagt atcaaaaatt gatgacagca
     5221 atgcgaatgc agaacaaaaa taggacgatt tgttggtt
//
LOCUS       DANLXF010000168         5217 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.168, whole genome shotgun sequence.
ACCESSION   DANLXF010000168 DANLXF010000000
VERSION     DANLXF010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5217
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.168"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            30..2299
                     /locus_tag="PXD66_001947"
                     /pseudo
     CDS             30..2299
                     /locus_tag="PXD66_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991033.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(2377..3078)
                     /locus_tag="PXD66_001948"
     CDS             complement(2377..3078)
                     /locus_tag="PXD66_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HDL6320215.1"
                     /translation="MDLQNRENFLNKLAQRMGRPRQNVPEPIPVLENNHAITRLTDRP
                     QPQLCQEFIDFARVMMADVVEAKEADLPQAIIDICEKYGGASVIVNNDQRLKDLGITA
                     AIQAKYEDSYVWDFNQAETNFEKARTANIGVVYGEYGLAESGGIVLFSDKEFGRSVSL
                     LPEKSVVVLRKSTVLPRVAQLAQILHEKAQRGERMPSCVNIISGPSATADIELIKVVG
                     VHGPVNKIYLVIDDL"
     gene            complement(3083..4492)
                     /locus_tag="PXD66_001949"
     CDS             complement(3083..4492)
                     /locus_tag="PXD66_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HDL6320216.1"
                     /translation="MSYLQTNTLPFKQRVEQQVNNEIVRKALVKAQETIGANRQRMVD
                     ELGNWEEWRDDAKAIRNHVLANLDAYLYQLSEKVTQNGGHVFFAETAEEATDYIKNVA
                     KAKNAKKIVKSKSMVTEEIGMNHVLEAEGIKVVETDLGEYLLQIVGDKPSHIVVPAIH
                     KDRHRIRQEMHDVLGYQGSETPEEMTAFVRQKIREDFLEADIGISGCNFAVPETGSVC
                     LVTNEGNLRMATTVPKTHIAVMGMERIAPTFKEVDVLITMLARSAVGAKLTAYNTWLT
                     GPRLEGETDGPEEFHLVIVDNGRSKILESEFQEVLRCIRCGACLNTCPAYRQIGGHGY
                     GSIYPGPIGSVISPLLGGYEEFKELPYACSLCTACNSVCPVKIPLAQLILKHREKIAD
                     AGLTPMAERLSIFGFNFANSHPTLWKVGVKVGAKVASKLIKNGKAPVNFGALGEWTKA
                     RDLPTAEGESFREWFNNRG"
     gene            complement(4494..5186)
                     /locus_tag="PXD66_001950"
     CDS             complement(4494..5186)
                     /locus_tag="PXD66_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HDL6320217.1"
                     /translation="MKAGVAKNTVLLLEKLGCKITFLEKQGCCGQPAINSGYTKQALA
                     GMKNLVETFEANDYPIVAPAGSCVYAIKTYPDHFERFGEKEWAERAKKVADRFYDLTD
                     FIVNKLGVKDVGAYLPGKAVYHPSCSLFRKLGIKDEPIALLQHVKGLELLPIKNQETC
                     CGFGGTFSVKMAEISGEMVKEKVANVDTDEPDYLIGADVSCLMNIGGRLSREGKPIKV
                     MHIAEVLMQGGK"
BASE COUNT         1523 a         1159 c         1006 g         1529 t
ORIGIN      
        1 tgttaaagaa aaattatctc actgtatcaa ttctccttgc cattagtgga gtaggctacg
       61 ctaatgaaat ctctttagag actattactg ttgatggaaa tacgccttca accaaaggga
      121 aattactagg tggtgaacta aatagcaatg aatctgttgt tgatgaaaaa aattaaaaca
      181 aggtagtatt acattaggta acgctttatc tggtgaatta ggcattcatt caagccagtt
      241 cggaggcggt gcaagtaccc caattattcg tgggcaagag agtaaacgtg caaaaatctt
      301 acaaaataat ggcgaaaact tagatatgtc gggaatgtct cctgatcatg cagtaaccgt
      361 agatgcgcta cttgctaaac gtattgaaat tttacgtggt ccaactactt tactttacag
      421 tgcaggtaat acggcaggtg taattaatgt ggtagacaat aaaattccaa ccgctattcc
      481 cgaaaaaggc tatgaggggc aatttggtgt gcgttttggc tcagccagta aagagcgttt
      541 aacctatgca ggctcaacct ttgcccttgg aaatcattta gccttgcggg tacagggaat
      601 gtataacaaa gcttccgaat actatgctcc acactttacg attgaaggca aaccttacca
      661 ccgcgtacca gatagcgatg tacaatcaca aaccggcaca gtaagccttt cttggattgg
      721 agagcgagga catcttggta ttgcttacac tgatcgccgt gataaatatg gattaattgg
      781 tcacacacac aaatacgatc actatactat tagtattatt cgccaagccg ttatgtttgc
      841 aaaaggttat ttacgttttt atccgcacct tgcagaagaa ggagatattg actataacaa
      901 ccctggtatc cgtttactgc atactcatat tccgggaggc tcacattatg gacaagatac
      961 tcacgagcac ggcaaacctt ggattgatat gcactccaag cggtatgata ttgatggaag
     1021 tttgcaaaat ccacttcctg gttttgaaga agccaaaata tcagctaact atgtagatta
     1081 ctatcatgat gaaaaagatg gaaaaagagt tgaaaattac ttcaagaaca aaggaaaaaa
     1141 tctacgtttc gaattggttc acaaagagtg gaaagggcta aaaggagcta ttggtgtgca
     1201 atataccaac caatctacct cagcacttgc acttgaagct tcacgagctg caaaagtatt
     1261 taataaacag cctttactca ataatccaaa aacaaaatta tggagtctct ttgcgattga
     1321 acgccttaat ttaggtgact ttacctttga gttaagtggg cgggcagaac gtcaaaaaat
     1381 agcaatggat tatgatgtga aattaattga tcgctggcta ggctttaata cacctatgcc
     1441 aaacttggac ccacataaag acaaaggtta ttcttatagc tttgcaactc attggtattt
     1501 cgcaccaaac cataagttaa cattaaatgc tgcacatcaa gaacgtttac ctaatgctca
     1561 ggaactctat gctcatggta aacacattgc tttaaatgca tttgaggctg gcaataaaaa
     1621 tctaaaaaaa gagcgttcta accaaattga gctatcatta gcttatgttg gcgacaaatg
     1681 ggattataaa ttaaatctct accacacccg ctatggcaac tatatttacc cattaactct
     1741 taatgataat cgtggaccta aatcttttac cgatgaatat aacttgaaag tgaatagata
     1801 ttatcaaggc gaagctcgtt ttagtggggc agaaggcgaa attggttatc tatttacacc
     1861 aaattatcgc ttagccgtat tcggagacta tgtccgtgga aagttagtca acttacctaa
     1921 tattgcaatg agttacaaca tttggacagg tgaagttgac aaatgggctt ctcagccaga
     1981 catttctgca cctcgtatac caccactacg tttaggagca agatttaatg ctgacttcaa
     2041 cctaaactgg tctggaatgt tagaatatta ccgagtattt gctcagaaaa aagtctcgaa
     2101 atacgaacaa gttacccctg gccatcatca agtaaacctt ggcgtaacct atagtaatca
     2161 ttttaatcaa acagaatacc aagtattcct taaagtggat aacttgctta atcagaaaat
     2221 gtaccaacat gcctcatatc taccacatat tccacaaatg ggtagaaatg caatgcttgg
     2281 aatgaatatc agtttttaat ttgtaaatct ctacaaaaat ataaccgctt gtaaatttct
     2341 atctacaagc ggttactttc agtggatttt ttgcaattag agatcgtcaa ttaccaagta
     2401 aattttattt accgggccgt gaacgccaac tactttaatc aactcaatat ccgcagtcgc
     2461 cgatgggcct gagatgatat tcacacaaga tggcattctt tcacctcgtt gggctttttc
     2521 gtgcagaatt tgtgcaagct gtgccacacg aggtaacact gtacttttgc gtaacacaac
     2581 caccgatttt tcgggcaata agctcactga acgcccaaat tctttatcgg aaaacagtac
     2641 aataccgcca gactctgcca agccatattc accgtaaacc acaccgatat tagcagttct
     2701 ggctttttca aagttggttt cagcttggtt gaaatcccac acatagctgt cttcgtattt
     2761 tgcttgaatg gctgcggtaa tcccaagatc ctttaaacgt tgatcgttat tcacgatcac
     2821 actcgccccg ccatattttt cacaaatatc aataatggct tgtggtaaat ccgcctcttt
     2881 tgcctctact acatctgcca tcatcactcg ggcaaaatcg ataaactctt ggcaaagttg
     2941 tggttgaggg cgatcggtta aacgggtaat cgcgtggttg ttttccaaca caggaattgg
     3001 ctcaggcaca ttttggcgtg gtctgcccat acgctgtgcc aatttattta agaaattttc
     3061 acgattttgt aaatccattt tcttatcctc tgttattaaa ccattcacgg aaactttcgc
     3121 cttcggcagt cggtaaatca cgggctttcg tccattcacc taacgcccca aaattcaccg
     3181 gtgctttgcc gtttttaatc aatttgcttg ccactttcgc ccccactttt acgccgactt
     3241 tccaaagggt tgggtgtgag ttagcgaagt taaagccaaa aatagacaag cgttctgcca
     3301 ttggcgttaa gccggcatcc gcaattttct cacggtgttt taagataagc tgtgccagtg
     3361 gaattttcac tggacaaaca ctgttacaag cggtacaaag tgagcaagca tacggcaatt
     3421 ctttaaactc ttcgtagcca ccgagcaatg gcgaaataac tgaaccaatc gggcccggat
     3481 agattgaacc atagccgtga ccgccaattt ggcgatacgc cgggcaagtg ttcaaacatg
     3541 caccacagcg gatacaacgc aacacttctt ggaattcgct ttctaagatt ttagaacgac
     3601 cgttatcgac aatgaccaag tggaactctt ccggaccatc ggtttcgcct tcaagtctag
     3661 gacctgtcag ccaagtgttg taagcggtga gttttgcccc taccgcactg cgagccagca
     3721 tggtaattaa tacatcaacc tctttaaagg ttggggcgat acgttccata cccattactg
     3781 caatatgcgt tttcggcaca gttgttgcca tacgcagatt accttcgttg gtcactaaac
     3841 aaaccgagcc ggtttccggc accgcaaagt tacagcctga aataccgatg tctgcctcta
     3901 agaaatcttc acggattttt tggcggacga atgcagtcat ctcttcaggt gtttccgaac
     3961 cttgataacc tagcacatcg tgcatttctt ggcggattct gtggcgatct ttgtggattg
     4021 caggtacaac aatatgagaa ggtttatcgc ccacaatttg caataaatat tcgcccaaat
     4081 cggtttcaac cactttaatg ccttctgctt ctaaaacgtg gttcatccca atctcttcgg
     4141 ttaccatcga cttcgatttt acgattttct tcgcattttt agctttcgcc acgtttttga
     4201 tgtaatcagt ggcttcttcg gcggtttcgg caaagaaaac gtgtccgccg ttttgggtga
     4261 ctttttcgct taattggtag agataagcgt ctaaattggc taaaacgtgg ttacgaattg
     4321 ctttcgcatc atcacgccac tcttcccagt tgcccagctc atccaccata cgctgacggt
     4381 ttgcaccgat tgtttcctga gccttaacta aggctttacg cacaatctcg ttattaactt
     4441 gctgctcaac acgttgttta aacggcaggg tgttagtttg taaataagac atcttatttt
     4501 cctccctgca ttaacacttc ggcaatgtgc atcactttga ttggcttacc ttcacggctt
     4561 aaacgaccgc caatgttcat caagcagctt acatctgccc cgattaaata atccggctca
     4621 tcggtatcta catttgcgac tttctctttt accatctcgc cggaaatttc tgccattttg
     4681 accgagaatg tgccaccaaa accacagcaa gtttcttggt ttttgattgg taataactct
     4741 aaccctttaa cgtgctgtaa taaagcaata ggctcatctt taatccccag cttacggaat
     4801 aagctgcaag acgggtgata aactgctttg cccggtaaat aagccccaac atctttcacg
     4861 cccagtttgt tcacaataaa atcggttaaa tcatagaaac gatcagccac tttcttcgca
     4921 cgttctgccc attctttttc gccaaagcgt tcaaaatgat ccggataggt tttgatcgca
     4981 tatacacaag aacctgccgg ggcgacaatc gggtaatcat tagcttcaaa ggtttcaacc
     5041 aagtttttca tacctgctaa agcttgctta gtgtaaccgc tgttgatcgc aggttgtcca
     5101 cagcaacctt gtttttctaa gaaagtgatt ttacaaccta atttttccaa taaaagcaca
     5161 gtattttttg caacccctgc tttcattaca tctgcaatgc aggttacgta aaaatta
//
LOCUS       DANLXF010000169         5205 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.169, whole genome shotgun sequence.
ACCESSION   DANLXF010000169 DANLXF010000000
VERSION     DANLXF010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.169"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..1264)
                     /locus_tag="PXD66_001951"
     CDS             complement(<1..1264)
                     /locus_tag="PXD66_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL6320218.1"
                     /translation="MTACHEPKRSIWQQIFTKNMLLCVYTGFCSGLPLFVLIQLMPAW
                     LTSANLDIKTIAAFTLTSLPYTWKFLWAALLDRYFPPFLGRRKSWIFISQIGLLVILA
                     SFGLFNPVQDISIIVTLSVLLAFLSATQDIVVDAFRREILSDNELGLGNSIHVNAYRI
                     AGLIPGGLSLFLADHYAWDTVFLITAAFLVPCLFVTLIIAAEPNHKPIDRSKPFYMAF
                     VDPFKEFFTRKGVAGAIGLILFIFLYKLGDSLATTLQTKFILDMGFTKSHIAGIVKIT
                     SLWCSIGGGIIGGVAMLKLGVNRALWLFGFVQLITILGFAYLASFEHFPTESIGAAEL
                     WKLGLVMAGEYLGVGLGTAAFVAFMARETNPAYTAMQLAIFTSLAALPSKMLGAYTGH
                     LVEMMGYYQFFWLCFFIGIPGMLMLFKVA"
     gene            1430..5146
                     /gene="metH"
                     /locus_tag="PXD66_001952"
     CDS             1430..5146
                     /gene="metH"
                     /locus_tag="PXD66_001952"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDL6320219.1"
                     /translation="MNTQHRTELLQNALTERILILDGAMGTMIQKYKLTESDFRGERF
                     KNSTIDLKGNNDLLTLTQPLLISAIHEKYLAAGADIIETNTFSATTIAQADYELESIA
                     YELNLVGAKLARLAADKFSIPEKPRFVAGILGPTNRTCSISPDVNDPGFRNITFMQLV
                     EAYAEATRGLIEGGADLIMIETIFDTLNAKAAVFAIETVFDELGVKLPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAKPLTFGLNCALGPKELRPYVEMMSKISETFVSVHPNAG
                     LPNAFGGYDLGAEEMATYIKEWAELGWLNIVGGCCGTTPEHIQAFAEATKGVAPRKLP
                     EIPTAMRLSGLEPLTIDDNSLFVNVGERNNVTGSAKFKKLIKEEKFAEAIEIAISQVE
                     NGAQVIDVNMDEALLDSKKCMTRFLNILATEPEAAKVPIMIDSSKWEVIEAGLQTVQG
                     KPIVNSISLKEGEEKFIHQAKLCRRYGAAVVVMAFDEVGQADTEERKVEICTRAYRIL
                     VDQLGFPPEDIIFDPNIFAIATGIEEHNNYGVDFIQACERIKRDLPHAKISGGVSNVS
                     FSFRGNNVVREAIHAVFLYYAIKAGMDMGIVNAGQLAIYDDLDPELREAIEDAVLNHR
                     HDTTDRLLEISEKYRGVKVESADESAAEWRNLPVAERLKHALVKGITTYIIEDTEEAR
                     QQFASPLEVIEGPLMAGMDVVGDLFGDGKMFLPQVVKSARVMKQSVAYLEPYINATKQ
                     KGSSNGKMVIATVKGDVHDIGKNIVSVVLQCNNFEVIDLGVMVPADVIIETAIREKAD
                     LIGLSGLITPSLDEMEYFLGEMNRLKLDIPVLIGGATTSKEHTAIKLYPKYNYPVVYT
                     ANASRCVTVCATLMNPEAKAEFWEKTKAEYEEIQRKFAVRKPLRNGLSIEEARANRFD
                     GFSGEWADYVPPTPKQTGIIEYKNVPIATLRKFIDWSPFFMLWGLMGGYPDAFDYPEG
                     GEEARRVYNDAQRVLDEFEQNGKLNPSGIMGIFPAVREGDDIAILNEDRTACIGKSYH
                     LRQQSERGKNSKSPYNLCLSDFIADRESGQQDWFGMFAVCAGIEEHYLVESYKAAGDD
                     YNAILLQAVGDRLAEAMAEYLHFELRTKVWGYTVEDEDNGRLIREEYIGIRPAPGYPS
                     CPEHTEKQIIWDLLEVEQRIGMKLTESYAMWPAASVCGWYFTHPTSNYFTLGKIDEDQ
                     VLDYAKRKGWDELTAKKWLSVAMKQDVKSQAENI"
BASE COUNT         1515 a         1207 c         1282 g         1201 t
ORIGIN      
        1 gagcaacctt aaacaacatt agcattcccg gaataccaat aaagaaacag agccagaaga
       61 attggtaata gcccatcatt tccactaaat gccctgtgta tgcccctaac attttgcttg
      121 gtagggcagc aaggctggtg aaaatggcga gctgcattgc agtgtaagcg ggattagttt
      181 cacgagccat aaaggcaacg aaagccgctg tgcctaaacc cacgcccaga tattcacctg
      241 ccatcactaa gcctaatttc caaagctcgg cagctccaat gctttcggta gggaaatgct
      301 caaaactggc aaggtaagcg aagcctaaaa tagtaattaa ttgtacaaat ccgaacagcc
      361 ataaggctcg gttaacgccc aatttcaaca ttgccacgcc accaataata ccgccgccaa
      421 tgctgcacca aagtgaagtt attttcacaa tgccggcaat gtgcgattta gtaaaaccca
      481 tatccaaaat aaatttggtt tgtagcgtag ttgcaaggga atcgcctaat ttatatagga
      541 aaataaatag aatcagccca atcgctcctg ccacgccttt acgggtgaaa aactctttga
      601 acggatcgac aaacgccata taaaacggct tagaacggtc gattggctta tgattcggct
      661 cggcagcgat tattagcgta acgaataagc aaggcactaa aaaagcagcg gtaatcagaa
      721 atacggtatc ccacgcatag tgatcggcta aaaataatga aagcccaccc ggaatcagcc
      781 ctgcaatacg ataagcatta acgtggatag agttacctaa cccgagttcg ttatccgata
      841 aaatttcacg ccgaaaggca tccaccacaa tgtcttgcgt ggcggaaaga aaagctaata
      901 gcacagaaag agtaacaata attgaaatat cttgcactgg gttgaacaag ccaaagctgg
      961 ctaaaatcac caatagcccg atttgtgaaa tgaaaatcca gcttttgcgt ctgcctaaaa
     1021 aaggtggaaa gtagcgatct aacaatgcag cccacaggaa tttccacgtg tagggcaaag
     1081 aggtcagggt gaaggcggca atagttttaa tatctaaatt ggcactggtt agccaagccg
     1141 gcattagctg aattaacaca aacaacggta aaccggaaca aaagccggtg taaacacaaa
     1201 gcaacatatt tttagtaaaa atctgttgcc agatagagcg tttgggttcg tgacaagcgg
     1261 tcatattttt cctacatttt gcaaaataaa aaggggaaga actgagccaa tatagcataa
     1321 ttttaaaggc gactctaacc ctttccattt cgtcacagat aaggtttgac agccttgcga
     1381 catatttcta ttattccttt taatcaaact aaaaaactgg aagcagagta tgaacacgca
     1441 gcatagaacc gaattacttc aaaatgccct taccgaacgc attcttatct tagacggtgc
     1501 gatgggaacg atgatccaaa aatataaact aaccgaatct gatttccgag gtgaacgttt
     1561 taaaaacagt accatcgatc ttaaaggcaa taacgaccta ctcacactca cccagccact
     1621 cctgatttct gctattcacg agaaatattt agccgccggt gcggatatta tcgaaactaa
     1681 tacctttagt gcgacgacca ttgctcaagc agattatgaa cttgaaagta ttgcttatga
     1741 gctgaatctt gtgggggcaa aacttgcccg tcttgccgcc gataaattct ccatccctga
     1801 aaaacctcgt tttgttgccg gtattcttgg gccgactaac cgtacttgtt cgatttcccc
     1861 tgatgttaat gaccccggtt tccgtaacat tacctttatg caactggtgg aagcctatgc
     1921 cgaggcaacc cgtggtttga ttgaaggtgg ggcggatctg attatgatcg aaaccatttt
     1981 cgataccctg aatgccaaag cggcggtgtt tgcaattgaa acggtgtttg atgaactggg
     2041 cgtgaaattg ccgataatga tctccggcac cattaccgat gcctcagggc gaacgctgtc
     2101 agggcagacc acggaggcgt tttataactc gctccgccac gccaaaccgc tgacgttcgg
     2161 cttgaactgt gcgctcgggc cgaaggaact ccgcccttat gtagagatga tgtcgaaaat
     2221 cagtgagact tttgtttcgg ttcacccgaa tgccggcttg ccaaacgctt ttggcgggta
     2281 cgatttaggg gcggaagaga tggcgactta catcaaagag tgggcagaac tcggctggct
     2341 gaatattgtc ggcggctgtt gtggcaccac gccggagcat atccaagcct ttgccgaagc
     2401 aaccaaaggc gttgcgcctc gtaaattacc tgaaattcca accgctatgc gtttgtcggg
     2461 cttagaaccg ctgacgattg acgataattc gctgtttgtg aatgtgggcg aacgcaataa
     2521 cgtcaccggt tcggcaaaat ttaagaagct gattaaagaa gaaaaatttg cggaagcgat
     2581 tgaaattgcc atcagccaag tggaaaacgg ggcgcaggtg attgacgtga atatggacga
     2641 ggcgttgctc gattccaaaa aatgtatgac ccgtttcctc aacattcttg ctaccgaacc
     2701 tgaggcggca aaagtgccga ttatgattga ctcctcgaaa tgggaggtga tcgaggcggg
     2761 cttgcaaacg gtgcagggca agccgatagt gaactccatt tcactcaaag agggcgaaga
     2821 gaaatttatt caccaagcca aactctgccg ccgctatggg gctgcggtgg tggtgatggc
     2881 atttgacgaa gtcgggcaag cggataccga agaacgcaag gtagaaattt gtacccgagc
     2941 ttatcggatt ttggttgatc agctcggctt cccaccggaa gacattattt tcgacccgaa
     3001 catttttgcg attgccaccg gtattgaaga acacaataac tacggcgtgg attttatcca
     3061 agcctgtgag cggattaaac gagacttgcc acacgccaaa atttccggcg gggtgtcgaa
     3121 tgtctctttc tctttccgag gcaacaatgt ggtgcgtgag gctattcacg ccgtgttcct
     3181 ctactatgcg atcaaagccg gaatggatat ggggatcgtg aacgcagggc agttggcgat
     3241 ttatgacgac ctcgaccctg aactgcgaga ggcgattgaa gatgcggtgc tgaaccaccg
     3301 tcatgacact accgaccgcc tgttggaaat ttccgaaaaa tatcggggcg taaaagtaga
     3361 aagtgccgat gaaagtgccg ccgagtggcg aaatttacct gtggcagagc gtttaaaaca
     3421 cgccctcgta aaaggcataa cgacctacat tattgaagat accgaagagg ctcgccagca
     3481 atttgcctct ccgctggaag tgattgaagg gccgctcatg gcgggtatgg acgtggtggg
     3541 cgacctgttc ggagacggca aaatgttcct gccgcaagtg gtgaaatcgg ctcgggtaat
     3601 gaaacagtcc gttgcctatt tagagccgta catcaacgcc accaagcaga aaggatcgag
     3661 caacggcaaa atggtgatcg ccaccgtgaa aggtgatgta cacgacatcg gcaaaaacat
     3721 cgttagcgtg gtgttgcagt gcaataactt tgaagtgata gacttaggcg tgatggtgcc
     3781 tgccgatgta attatcgaaa ctgctattcg ggagaaagcc gatctcattg ggttaagcgg
     3841 actaattacc ccatcgctgg acgaaatgga gtatttttta ggtgaaatga accgcttgaa
     3901 gctcgatatt ccggtgctga ttggcggcgc aacgacctca aaagagcata cggcgatcaa
     3961 actttatccg aaatataact accccgtagt ctataccgcc aacgcttcac gttgcgtaac
     4021 cgtctgtgcg accttgatga accctgaggc aaaagccgaa ttttgggaaa aaaccaaagc
     4081 cgaatacgaa gaaattcagc gtaaatttgc cgttcgcaaa ccacttcgca acggtttatc
     4141 tattgaggag gctcgagcca atcgttttga cggatttagt ggcgaatggg cagactatgt
     4201 gccgccaacg ccaaaacaga ccggcattat cgagtacaaa aatgtaccga ttgccaccct
     4261 acgcaaattt atcgactggt cgccgttctt tatgctctgg ggcttaatgg gcggttatcc
     4321 tgatgccttt gattatccgg aaggcggcga agaggcacgc cgtgtctata acgatgccca
     4381 acgggtgctg gacgaatttg agcaaaacgg caagctcaac ccaagtggga ttatgggcat
     4441 tttccctgcc gtgcgtgagg gcgatgatat tgcaatttta aacgaagatc gcaccgcttg
     4501 tatcggcaaa tcctaccacc tccgccaaca atccgaacga ggcaaaaaca gcaaaagccc
     4561 gtacaacctc tgtttaagcg attttatcgc cgaccgagaa agcggacaac aagactggtt
     4621 cggaatgttc gccgtttgtg ccggcattga agaacactat ttagttgaaa gctacaaagc
     4681 ggcaggcgat gactacaacg ccattttgct ccaagccgtt ggcgaccgct tggcagaagc
     4741 aatggcggaa tacctacact ttgagctacg taccaaagtc tggggctaca ccgtggaaga
     4801 cgaagacaac ggacgcttaa tccgtgaaga atacatcggc atccgccctg cgcccggcta
     4861 cccaagctgc ccggaacaca ccgagaaaca gataatttgg gacttgttag aggtggaaca
     4921 acgcatcggc atgaaactca ccgaaagcta tgccatgtgg ccggcagcaa gtgtctgcgg
     4981 ctggtacttc acccacccta ccagcaacta cttcactctc ggcaagattg atgaagatca
     5041 ggtgttggat tacgccaaac gcaaaggctg ggacgaactc acggcgaaga aatggttaag
     5101 tgtggcgatg aaacaagatg tcaagtcgca ggctgaaaac atttaatgta gtggcgggtt
     5161 ttaacccgcc tgttacaagc ggttggtttt gagataaaat atgca
//
LOCUS       DANLXF010000170         5203 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.170, whole genome shotgun sequence.
ACCESSION   DANLXF010000170 DANLXF010000000
VERSION     DANLXF010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5203
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.170"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..156
                     /locus_tag="PXD66_001953"
     CDS             <1..156
                     /locus_tag="PXD66_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HDL6320220.1"
                     /translation="DLTTLQADLDEWLMYYNHHRTHQGKMCCGRTPMATLLDGKRIWV
                     EKNLSSN"
     gene            215..799
                     /locus_tag="PXD66_001954"
                     /pseudo
     CDS             215..799
                     /locus_tag="PXD66_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006718099.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
     gene            883..1653
                     /locus_tag="PXD66_001955"
     CDS             883..1653
                     /locus_tag="PXD66_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDL6320221.1"
                     /translation="MTITNTELEQILNTKLNSSAINDYAPNGLQVEGKREIKKIITGV
                     TATLPLIEKAIEKNADAILVHHGYFWKSENPCIRGMKGKRIKQLLVNDINLFGYHLPL
                     DIHSELGNNAQLAKHLGVKNLQGLEDRPNSIPMYGELENPISANELKQKLEKTLQRTV
                     ILCDEFVHSPQKMIKTIGICSGGGQGYIDLAFEKGCDAFISGEISEQTTHSAREQGIY
                     YFACGHHATERDGVKALGEWLAVEYGLDVEFIDIDNPA"
     gene            complement(1734..2864)
                     /gene="nrdB"
                     /locus_tag="PXD66_001956"
     CDS             complement(1734..2864)
                     /gene="nrdB"
                     /locus_tag="PXD66_001956"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HDL6320222.1"
                     /translation="MAYTTFSQHKNDQLKEPMFFGQNVNVARYDQQKYEIFEKLIEKQ
                     LSFFWRPEEVDVSQDRIDYAQLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWIETWTFSETIHSRSYTHIIRNIVNDPSIVFDDIVTNEEIIKRAKDISAYYDD
                     LIRDSQLYSLYGEGSYQVDGKTCEVSLRSLKRQLYLCMMSVNALEAIRFYVSFACSFA
                     FAERQLMEGNAKIIKFIARDEALHLTGTQHILNIMAYGNDDPEMAEIARECRQEAYEL
                     FLSAAEQEKAWAEYLFKDGSMIGLNRDILVQYIEYITNIRMQAVGLPLPFKARSNPIP
                     WINAWLVSDNVQVAPQEVEVSSYLVGQIDAKVDSRDFGDFEL"
     gene            complement(2891..5161)
                     /gene="nrdA"
                     /locus_tag="PXD66_001957"
     CDS             complement(2891..5161)
                     /gene="nrdA"
                     /locus_tag="PXD66_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018356743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HDL6320223.1"
                     /translation="MNKSLMVTKRNGTREQLNLDKIHRVITWAAEGLENVSVSQVELR
                     SHIQFYEGIRTSDIHETIIKAAADLITKDTPDYQYLAARLAIFHLRKKAYGQFEPPHL
                     YEHVKKLVALGKYDADLLSDYTPEEWETMNGFIDHWRDMTFSYAAVKQLEGKYLVQNR
                     VSGEIYESAQFLYLLVAACLFSKYPTASRLDYIRRFYDATSTFKISLPTPIMAGVRTP
                     TRQFSSCVLIECDDSLDSINATSAAIVKYVSQRAGIGINAGAIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATVYFPIWHLEAESLMVLKNNRGVEENRARHMD
                     YGVQINKMMYQRLIKGGEISLFSPSDVPGLYEAFFADQAKFEQLYLQYEQDPSIRQRK
                     VKAVELFSLLMQERASTGRIYIQNVDHCNTHSPFNPRVAPIRQSNLCLEIALPTKPLA
                     HFYDEQGEIALCTLSAFNLGTLSNLDELEELADLVVRALDALLDYQDYPVLAARNSSL
                     NRRALGVGVINYAYYLAKNQVRYSDGSANNLTHRTFEAIQYYLLKASMNLAKEQGACA
                     YFDQTTYAKGLLPIDTYKKEIDELTQEPLHYDWEQLRQDILQFGLRNSTLTALMPSET
                     SSQISNATNGIEPPRGYISVKASKDGILKQVVPEYERLKAYYELLWDIPNMDGYLHLV
                     GIMQKFVDQAISANTNYDPNRFAEAKVPMKVLLKDLLTAYKYGLKTLYYQNTRDGAED
                     VQDDLDEGCAGGACKI"
BASE COUNT         1622 a         1083 c          966 g         1532 t
ORIGIN      
        1 gatttaacga cattacaagc ggatttagat gagtggttaa tgtattataa tcaccatcga
       61 acacatcaag gaaaaatgtg ctgtggcaga acaccgatgg caaccttact tgatggaaaa
      121 cggatttggg tggaaaagaa tttaagctca aattaatctg acagacacgg taatttaaaa
      181 cgggggactg tcagattagg tttgatcttc tacaccttat accactatta tgcaacgcat
      241 ttcaggtgca aaacagatag attcactcac ggttaaaatt aaagatgata ttgaaagcca
      301 aactgccgaa aaaagcattg ccgaattgtt agtagctaaa cacggcaaaa aagatttctt
      361 tattatgaat agtgatacga tcaaacaaac cattaccagt accaccaata caatgacttt
      421 gctgatctcc tctatcgccc ttatttcgct gattgtcggt ggtatcgggg taatgaatat
      481 tatgctcgtt tccgtcaccg aacgcacccg agaaattggt gtccgaatgg cgattggcgc
      541 aaaacaacgc aatattttgc aacaattttt aattgaggcg gtggttattt gcctaatagg
      601 tggtgtgatt ggcattttac tggcaagcgc tattatttgg gcatttaata ccttaggtac
      661 gaattttaaa atgatgcttt caccggaatc ggtgattatt gccgtgctct gctctacact
      721 tatcggtgtg gtatttggct acattccggc aagaaacgcc gcccaattaa acccaattac
      781 tgctctggca caagagtaaa tgcgataact atctgctaca ctgtaggggc aaaaaatttt
      841 ttgcccctac atccattatt atgccaagtt taaggagata aaatgacaat aaccaatact
      901 gaactagaac aaattctgaa caccaaactc aacagctccg ccattaacga ctatgcccca
      961 aatggcttgc aagttgaagg gaaaagagag atcaaaaaaa ttattaccgg tgtgacagct
     1021 acacttcccc tgattgaaaa agcgattgag aaaaacgccg atgcaatttt agtccaccac
     1081 ggttattttt ggaaaagtga aaacccgtgc attcgaggaa tgaaaggtaa acgcattaaa
     1141 cagcttttag tgaatgacat caatctcttt ggttatcacc taccactgga tattcactcc
     1201 gaactaggta acaacgccca gcttgctaag catttaggag taaaaaatct acaaggttta
     1261 gaagacagac ctaattcaat tccaatgtac ggtgaattag aaaatccgat ttccgccaat
     1321 gaactgaaac aaaaacttga aaaaacgtta caacgcaccg ttattttatg tgatgaattt
     1381 gttcatagcc cacaaaaaat gattaaaaca atagggattt gctccggcgg aggacaaggc
     1441 tatattgatt tagcctttga aaaaggctgt gatgccttta ttagtggcga aatttccgaa
     1501 cagaccactc actctgcccg tgaacaagga atctattatt ttgcttgcgg tcatcacgcc
     1561 acggaacgag atggcgtaaa agccttaggt gaatggttag ccgttgaata tggtttagat
     1621 gttgaattta ttgatattga taaccctgca taaaaaatac aagcggttga atttcctgaa
     1681 aattttgcaa tttgcaaaaa atcactaaaa atcaaccgct tgtatatcat agtctaaagt
     1741 tcaaaatcgc caaaatcacg gctatctact ttagcatcaa tctggccaac caaataagaa
     1801 ctgacttcca cttcttgtgg tgctacctgt acattatcgg aaaccaacca cgcattgatc
     1861 cacggaatgg ggttggaacg tgccttaaat ggtaatggta gtccaacagc ctgcatccga
     1921 atattagtga tatattcaat atattgcacc aaaatatccc gatttagccc tatcatcgaa
     1981 ccgtctttaa acaggtattc cgcccacgct ttttcttgtt cagctgcact taaaaaaagc
     2041 tcgtaggcct cttggcgaca ttcacgtgca atttctgcca tttcaggatc atcattgcca
     2101 taggccataa tattcaatat atgctgagtg ccggtaagat gtaatgcttc atcacgtgca
     2161 ataaatttga tgattttggc atttccttcc ataagctgcc gttccgcaaa ggcaaatgag
     2221 caagcaaaag agacataaaa acgaattgct tcaagcgcat tcacactcat catacagaga
     2281 taaagttgtc tttttagact acgcaaactg acctcacagg tttttccatc aacctgataa
     2341 ctgccttcac catacaaact atagagttgg ctgtcacgaa tcaaatcatc ataataggcg
     2401 gaaatatctt tggcacgctt aataatttct tcgtttgtga caatatcatc aaatactatt
     2461 gatggatcat tgacaatatt acggatgata tgtgtgtaag aacgactgtg aattgtttcg
     2521 ctaaatgtcc aagtttcaat ccaggtttca agctcgggga tagacactaa tggcaaaagc
     2581 gccacattcg ggctacgccc ttgaatggaa tccaacaagg tttggtattt taaattgctg
     2641 ataaaaatgt gcttttcatg ttcaggcagt tgggcatagt caatgcggtc ttgtgacaca
     2701 tccacttctt ccggtcgcca gaaaaatgag agttgttttt caatcagttt ttcaaaaatt
     2761 tcgtattttt gttgatcata acgcgccaca ttcacatttt gtccaaaaaa catgggttct
     2821 tttagttgat catttttatg ttgtgaaaac gtagtatatg ccatgttatt ctcctttatt
     2881 tctgttctta ttaaatttta caagcaccac cagcacagcc ttcatctaag tcgtcttgca
     2941 catcttctgc tccatcacgg gtattttgat agtaaagggt ttttaacccg tatttatacg
     3001 ccgttaatag atcttttaat aatactttca tcggtacttt agcttctgca aagcggtttg
     3061 ggtcgtaatt tgtattcgcc gaaatcgctt ggtcaacaaa tttctgcata atcccaacca
     3121 aatgcaaata tccgtccata ttaggaatat cccaaagcag ttcatagtat gcttttaaac
     3181 gttcatactc cggcacaacc tgctttaaaa taccgtcttt tgatgcttta acactaatat
     3241 agccacgcgg aggttcaatt ccattggtgg cattcgaaat ttgcgacgaa gtctccgatg
     3301 gcattaaggc agttaaagtg gaattgcgca agccaaattg cagaatatcc tgacgtagtt
     3361 gttcccaatc ataatgaagc ggttcttgag ttaattcatc aatttctttt ttataagtat
     3421 cgatagggag taaccctttg gcataagtgg tttgatcaaa ataagcacac gccccttgtt
     3481 ctttggctaa attcatcgat gcttttaata aataatactg aatcgcttca aaggtacggt
     3541 gagttaaatt attagcactg ccatcggaat aacgcacttg gtttttagct aaatagtaag
     3601 catagttaat cacgcctaca cctaatgcgc gacgatttaa tgagctgtta cgtgcagcta
     3661 ataccggata atcttgataa tctaataggg catctagcgc acgaaccact aaatcagcca
     3721 attcttctaa ctcatctaaa ttgctgagtg tgcctaaatt aaatgccgat aatgtacata
     3781 atgcgatttc cccttgttca tcataaaaat gtgccagtgg cttagttggc aaagcaattt
     3841 ctaagcagag gttcgattgg cgtatcggtg cgacacgagg attaaatggg ctatgagtat
     3901 tacaatgatc gacattctga atataaattc gccctgttga agcccgctct tgcatcagta
     3961 atgaaaacaa ttctacagct ttaactttac gttgccgaat gctcggatct tgctcatatt
     4021 gcaaataaag ttgctcaaat ttagcttgat cagcaaaaaa cgcttcatat aaccccggca
     4081 catctgatgg actaaataaa ctaatctcac cgcctttaat taaacgttga tacatcattt
     4141 tattaatctg taccccgtaa tccatatggc gagcacggtt ttcttccaca ccacggttat
     4201 tttttaatac cattaagctt tctgcttcca aatgccaaat cggaaaatac acggtagccg
     4261 ctccaccgcg aacaccacct tgagagcaag actttactgc agtttgaaaa tgtttataaa
     4321 agggaataca gccggtatga aatgcttctc caccacgaat ggggctacct aatgcccgaa
     4381 ttgcgccggc attaataccg atcccagcac gctgtgaaac atatttaacg attgcagccg
     4441 aggttgcatt gatggaatct aagctatcat cacactcaat taacacacag gaactaaatt
     4501 gacgagtagg tgtacgcact cctgccataa tcggtgttgg caaagaaatt ttgaaagtgg
     4561 aagtcgcatc ataaaaacgg cgaatataat ctaagcggct tgctgttgga tacttagaaa
     4621 ataaacaggc agctacgagt aaatacaaaa attgagccga ttcataaatc tctccgctaa
     4681 cacgattctg cactaaatat tttccttcca actgctttac tgctgcatag gaaaacgtca
     4741 tatcccgcca gtgatcaata aatccattca ttgtctccca ttcttcgggc gtatagtcgc
     4801 tcagcaagtc tgcatcatat ttacctagcg caactaattt tttaacgtgc tcatataaat
     4861 gcggtggctc aaattgtcca taagcttttt tgcgtaagtg gaaaatcgcc aagcgtgcgg
     4921 ctaaatattg ataatctggc gtatccttag tgattagatc agctgcagcc ttgataatag
     4981 tttcatgaat atctgaagtg cgaatacctt cataaaattg aatgtgagaa cggagttcta
     5041 cttgtgaaac agaaacattc tctaaccctt ctgccgccca agtaatcaca cgatgaattt
     5101 tatctagatt gagttgctcg cgcgtgccat tacgctttgt taccattaaa cttttattca
     5161 taattattaa ccctttataa aataccttag taaaacacta cat
//
LOCUS       DANLXF010000171         5178 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.171, whole genome shotgun sequence.
ACCESSION   DANLXF010000171 DANLXF010000000
VERSION     DANLXF010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5178
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.171"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..181
                     /locus_tag="PXD66_001958"
     CDS             <1..181
                     /locus_tag="PXD66_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HDL6320224.1"
                     /translation="EEKGYRIARKHVGWYTEKLNPESNFKRTFNTLSSTKEQLYALED
                     FVKSIHLESQLRKVG"
     gene            186..482
                     /gene="fis"
                     /locus_tag="PXD66_001959"
     CDS             186..482
                     /gene="fis"
                     /locus_tag="PXD66_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HDL6320225.1"
                     /translation="MLEQQPTQNPLTVTMLNAQAQQVNKPLRDNVKAALKNYLSQLNG
                     EDPTELYELVLSEIEHPMLDMVMQYTRGNQTRAATMLGINRGTLRKKLKKYGMG"
     gene            complement(587..3373)
                     /locus_tag="PXD66_001960"
     CDS             complement(587..3373)
                     /locus_tag="PXD66_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin family TonB-dependent
                     receptor"
                     /protein_id="HDL6320226.1"
                     /translation="MKYHHFRYSPVALTVLFALSHSYGAATENKKIEENNDLAVLDEV
                     IVTESHYAHERQNEVTGLGKVVKNYHEMSKNQILGIRDLTRYDPGISVVEQGRGASSG
                     YAIRGVDKNRVSLLVDGLPQAQHYHTLGSDANGGAINEIEYENIRSIELSKGASSAEY
                     GSGALGGAIGFRTKDAQDIIKEGQHWGLDSKTSYASKNSHFLQSIAAAGEAGGFEALV
                     IATHRHGKETKIHSEANKLKHNIRRITGFENRYDLYPNSAQNAPGGSFFIVEDTCPTL
                     DCTPRARVKLNRDNFPVRTFPEYTPEERKQLEQIPYRTEQLSAQEYTGKDRIAPNPLD
                     YKSNSVFMKFGYHFNSSHYLGAILEDTKQRYDIRDMQTPAYYTKDDINLSLRNYVYEG
                     DNILDGLVFKPRIPYGLRYSHVKFFDERHHKRRLGFTYKYKPENNRWLDSIKLSADKQ
                     DIELYSRLHRLHCSDYPVVDKNCRPTLDKSWSMYRTERNNYQEKHRVIHLEFDKALNA
                     GQGVFKQTHKLNLGLGFDRFNSLMDHGDMTAQYTKGGYTSYRGRGRLDNPYIYRRDPR
                     SIETVSLCNNTRGDILNCEPRKIKGDSHFVSFRDLVISEYVDLGLGVRFDQHRFKSDD
                     PWTLSRTYRNWSWNGGITLKPTEFVSLSYRISNGFRVPAFYELYGKRNHIGLKDNEYV
                     QRAQRSHQLEPEKSTNHEIGVSFKGQFGYLDVSYFRNNYKNMIATACKRIIQKSHCFY
                     NYHNIQDVALNGINLVAKFDLHGILSMLPDGFYSSVAYNRVKVKERKLTDSRLDSVND
                     PILDAIQPARYVLGFGYDHPEEKWGIGITTTYSKAKNADEVAGTRHHGIHRVDLGGKL
                     TGSWYTHDITGYINYKNYTLRGGIYNVTNRKYSTWESVRQSGVNAVNQDRGSNYTRFA
                     APGRNFSLAFEMKF"
     gene            complement(3467..>5178)
                     /locus_tag="PXD66_001961"
     CDS             complement(3467..>5178)
                     /locus_tag="PXD66_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HDL6320227.1"
                     /translation="LLAACSSNGGSFDVQSAKVESQTQTTPKKPSLQDDNSNARRTVS
                     ASETEALLQPGFGFSAKIPRRNLFPQGKEDVAPIGDIKEITGDLTKIPYEEEVKAYGS
                     SADGFSHTHDRNHKLYTRDFNFVRSGYVLHSGAKPEIKPKEILRTGAHGYVYYLGIEP
                     AKAIPTQKATYKGYWDFTTDARKGRDSKYFSNSAGINIGATPENSYDINVDDSEKPMG
                     HTGEFTADFANKTLTGTLVRNGYVSRSKEQKITTIYDIDAKIKGNRFSGKANAKNTDD
                     PYFGKSSTTLEGGFFGGKAQELAGKFLADDKSVFVVFAGKRDAKKDDSESAFDAFKIK
                     LKDLNKSEMDTFGNATHLIINNKQIPLIAEGKKSFAEMKFDDLVTRTIDDKTYRVSVC
                     CNNLDYVKFGTYSEGNNSDTAHQYLVGERTAVADLPTGTVKYRGTWDGVMYSKSGSAG
                     AESPSNSESGTRSLFDVDFVNKKINGKLIANDGVEERPMLTLEGNLKGNGFAGTAKTG
                     NSGFNLDPKSTNGGTVVHINTQFEGGFYGPKATELGGIVHSAETDKDRVSITFGGKRQ
                     IEK"
BASE COUNT         1542 a         1113 c          900 g         1623 t
ORIGIN      
        1 tgaagaaaaa ggttatcgta ttgcacgaaa acatgttggt tggtataccg aaaaattaaa
       61 tccggaatca aattttaaac gcacttttaa tacactaagt tccacaaaag agcaacttta
      121 tgcattagaa gattttgtta aatcgattca tttagaatct caattaagaa aagttggata
      181 aaaaaatgtt agagcaacaa cctacacaaa acccattaac agtaacaatg ttaaatgcac
      241 aagcgcagca ggttaataaa ccacttcgcg ataatgtgaa agcagcatta aaaaactatt
      301 tatctcaatt aaatggtgaa gatccaacag aattatacga attagtatta tctgaaatcg
      361 aacacccaat gttagatatg gtgatgcaat atactcgcgg caatcaaact cgtgcagcaa
      421 caatgctagg cattaaccgt ggcacattac gcaaaaaatt aaaaaaatac ggtatgggtt
      481 aatctaaccg accgttaaaa cacgatttta attcgtatgt aattagttag agccaacaaa
      541 aaacgctgaa tatactattc agcgtttttc tattttattt tgccgtctaa aacttcattt
      601 caaatgctaa actgaaattt ctccccggag cggcaaatcg agtgtaattg ctaccccggt
      661 cttggtttac tgcattcaca ccggattggc gcactgattc ccaagtggaa tatttacgat
      721 tagtcacatt ataaattcct ccacgtaagg tgtagttttt ataattgatg taaccggtaa
      781 tatcatgggt gtaccaagaa ccggtcagtt tgccacctaa atcaacgcga tgtatgccgt
      841 gatgacgtgt gcctgccacc tcatcggcgt ttttggcttt agaataggtg gtagtaatgc
      901 caattcccca tttttcttct gggtgatcgt agccgaatcc aagcacatag cgtgctggct
      961 gaatcgcatc tagaatagga tcgtttacgc tatcgagtct tgagtcggtt agtttccgct
     1021 cttttacttt tacacggtta taagcaactg atgaataaaa accatctggc agcatagata
     1081 aaataccgtg taagtcaaat ttagcgacta aatttatccc gtttagtgct acatcttgaa
     1141 tattatggta gttatagaaa cagtgtgatt tttgtattat tcttttacat gctgtcgcaa
     1201 tcatattttt atagttatta cggaaatagc tcacatcaag gtaaccaaat tgacctttaa
     1261 agctaactcc aatctcatga ttagtcgatt tttctggctc taactggtgg ctacgttgcg
     1321 cgcgttgcac atattcgtta tctttaagcc caatatgatt acgtttacca taaagttcat
     1381 agaatgcagg cactctaaaa ccgtttgaaa tgcgataaga aagcgataca aactctgttg
     1441 gtttaagcgt aatcccacca ttccaagacc aatttcgata agttcggcta agtgtccacg
     1501 gatcatcaga tttaaatcga tgttgatcaa aacgcacccc taatcccaaa tccacatact
     1561 cgcttatcac tagatcgcgg aagctaacaa aatggctatc gcctttaatt ttacgcggtt
     1621 cacagtttaa gatgtcgccg cgtgtattat tacacaaaga taccgtttca atactgcgtg
     1681 gatcgcggcg ataaatatat ggattatcta aacgccctct accgcggtag ctggtataac
     1741 cgcctttggt atattgggca gtcatatccc catgatccat aagcgaatta aatcgatcaa
     1801 agcccaaccc taaattcagt ttgtgggttt gcttaaatac gccttgacca gcatttagcg
     1861 ctttatcaaa ttctaaatga atgacacgat gcttttcttg gtaattatta cgctcagttc
     1921 gatacataga ccaagattta tccaaagtcg ggcggcaatt tttatctacc acaggataat
     1981 cgctacaatg caagcgatgt agccggctat atagttcaat atcttgttta tccgcactga
     2041 gtttaatgct atccaaccag cgattattct ctggtttata tttataggtg aatcctaaac
     2101 gacgtttgtg gtgacgttca tcaaaaaact tcacatggct atagcgcaac ccataaggga
     2161 tccttggctt gaacactaag ccatctaaaa tattatcccc ttcataaaca tagttcctaa
     2221 gtgataagtt aatatcgtct tttgtatagt aagctggcgt ttgcatatca cggatatcgt
     2281 agcgttgttt tgtatcttct aagattgcgc caagataatg agacgagtta aagtgatagc
     2341 caaacttcat aaaaacagaa ttactcttgt aatctaaagg gtttggtgca atgcgatctt
     2401 taccggtata ttcttgggct gagagctgct cagtgcgata aggaatctgc tcaagctgtt
     2461 tgcgctcttc aggcgtatat tccggaaatg ttctcactgg gaaattatcg cggttcaact
     2521 taacccttgc acgaggagta caatctaatg ttgggcaagt atcttccaca ataaaaaaag
     2581 atcctccagg agcattctgt gcggaatttg ggtaaagatc gtagcgattt tcaaagccgg
     2641 ttatacgccg aatattatgt tttaatttat ttgcctcgga atgaattttg gtctctttac
     2701 cgtgtcggtg agttgcaata acaagtgctt caaaaccacc cgcctcacca gccgctgcga
     2761 tagactgtaa aaaatggcta tttttgctgg cataagaggt cttactatct aagccccaat
     2821 gctgcccctc tttaataata tcctgcgcat ctttagtacg aaaaccaata gcaccaccta
     2881 gcgcaccaga gccatattcc gcagaacttg ctcctttgct taactcaatt gaacgaatgt
     2941 tttcatactc aatctcatta attgcaccac cattagcatc tgaacctagc gtatgataat
     3001 gctgcgcttg tggtagccca tcaacaagta agctgacacg gtttttatct acacctcgaa
     3061 tggcatagcc actacttgca ccgcgacctt gttccaccac cgaaatacca gggtcatagc
     3121 gagttaaatc acgaatacca agaatttgat ttttactcat ttcgtgataa tttttcacta
     3181 ctttccccaa gccagttact tcgttttgac gttcgtgagc ataatggctc tctgtcacaa
     3241 taacttcatc cagaacagct agatcgttat tttcttctat ttttttattt tcagtcgcag
     3301 caccgtatga atgagaaaga gcaaataaca ctgttaaggc aacaggtgaa tagcgaaaat
     3361 gatgatattt cattatcatt agatagggtc tcctttattt aaattttgca aatttttata
     3421 aaaaattgcc cgcttgctat ctacaaccag caaaggggaa ttatgattat ttttctattt
     3481 gacgttttcc gccgaatgta atactgactc tatctttatc cgtttctgca ctatgtacaa
     3541 taccacctaa ttccgtcgcc ttagggccat aaaagccccc ttcaaattga gtatttatat
     3601 gcactaccgt gccaccattc gtacttttcg gatcaagatt aaaaccagaa ttgcccgttt
     3661 tggctgtgcc tgcaaaacca ttccctttca gattgccttc aagtgtcagc attgggcgtt
     3721 cttcaacacc atcattagca atcagcttgc cattaatttt tttattgaca aaatctacat
     3781 cgaatagtga acgagtacca ctttcgctgt tacttggcga ttcagcccct gccgagccag
     3841 atttactgta cattaccccg tcccaagtac ctcgatattt tactgtccct gttggcaaat
     3901 ctgccacagc tgtacgttct cctactaaat attggtgagc agtatcacta ttatttccct
     3961 cgctataagt cccaaatttg acataatcta aattattaca gcagactgaa actcgatacg
     4021 ttttatcatc aatagtacgg gtaaccaaat catcaaattt catctcggca aagctttttt
     4081 tgccttccgc aataagtgga atctgcttat tgttaataat caaatgtgtc gcattcccga
     4141 aagtatccat ctcagattta tttaaatctt taagtttaat tttgaaagca tcaaaggcag
     4201 attcactatc atctttttta gcatctcgtt tgccagcaaa aacaacaaat accgacttat
     4261 catcagctaa gaatttaccg gcaagttctt gagccttccc accaaaaaat ccaccttcaa
     4321 gtgtcgtaga gcttttacca aaataaggat catcggtatt ttttgcgttt gctttaccag
     4381 aaaagcgatt acctttaatt tttgcatcaa tatcgtaaat tgttgtaatt ttttgctctt
     4441 tgctacgact aacataccca ttacgaacca atgttccagt taaagtttta ttagcaaaat
     4501 cagccgtaaa ttctcctgta tgccccattg gtttttcaga atcatcaaca ttaatatcgt
     4561 aactattttc cggtgtggcg ccaatattga tgcctgcgga attagaaaaa tatttactat
     4621 ctctcccttt tctcgcatcg gtagtaaaat cccaatatcc tttataagta gctttttgag
     4681 taggtattgc ttttgccggc tctataccta aatagtaaac atacccatgt gcacctgttc
     4741 tcaaaatttc tttaggcttt atttcaggtt ttgcaccaga atgcagaaca tagccggaac
     4801 gaacaaaatt aaaatctcta gtatacaact tatgatttct atcatgagta tggctaaatc
     4861 catcagcact actaccgtac gctttaacct cttcttcata cggaattttt gtcagatctc
     4921 cagtaatctc ttttatatca ccaatagggg ctacatcttc cttcccctgc ggaaagagat
     4981 tacgacgcgg aattttggct gaaaaaccaa accccggctg caataaagct tcagtttcag
     5041 aagcgcttac tgtacgtctt gcgttactat tatcatcttg taaacttggc tttttggggg
     5101 tagtttgcgt ttgagattca actttggcag attgaacatc aaagcttcca ccatttgagg
     5161 aacaagcagc aagtagcg
//
LOCUS       DANLXF010000172         5162 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.172, whole genome shotgun sequence.
ACCESSION   DANLXF010000172 DANLXF010000000
VERSION     DANLXF010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5162
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.172"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            162..623
                     /locus_tag="PXD66_001962"
     CDS             162..623
                     /locus_tag="PXD66_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HDL6320228.1"
                     /translation="MTACPCQTGKRYAECCQPFHLKQAFASTAEQLMRSRYSAYTLIN
                     IPYIVETTVPSQQKLLDQTAMKEWAENTKWVGLSVLNHLPSLSKIHSQVEFKAFFDTD
                     EGIQAHHETSLFVQIDKRWFFVDPTVPLPHQKQPCVCGSGKKFKHCCGNYL"
     gene            695..1324
                     /locus_tag="PXD66_001963"
     CDS             695..1324
                     /locus_tag="PXD66_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HDL6320229.1"
                     /translation="MDFSKILSQVLDTAKDAATNGLIKGNSKNDQIAKIGGGAAAIGL
                     ISMLFGRNGGSSLAKLGSLAALGSLAYQAYQKYQAQNASASELSQNSFENVDKNVASK
                     VILQAMIAAAASDGAITEEEKAAIISQVGDDVEVIEWIRQEMDSPASVEEIARQVGND
                     QALATQVYLAARAVCTNLERKEIVFLANLAEALGLAEALVEQLEKDAGF"
     gene            complement(1407..2054)
                     /locus_tag="PXD66_001964"
     CDS             complement(1407..2054)
                     /locus_tag="PXD66_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015702020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL6320230.1"
                     /translation="MISIRNVSKQYRTNNGWKTILKDINFELKKGEKVGILGRNGAGK
                     STLIRLMSGVEPPSTGTIERQMSISWPLAFSGAFQGSLTGMDNLRFVSRIYGADFDYV
                     KSFTEEFSELGEYLYEPVKNYSSGMKARLAFALSLSIEFDCYLIDEVIAVGDARFAAK
                     CKYELFEKRKDRSLILVSHSPASIKEYCDSAMVLEEGIMYTFDSIDNAYNFYNKT"
     gene            complement(2051..2848)
                     /locus_tag="PXD66_001965"
     CDS             complement(2051..2848)
                     /locus_tag="PXD66_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157140410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL6320231.1"
                     /translation="MRYGDNTTFKRSCVIQKRVIHALLMRELITRYGRKNIGFLWLFI
                     EPLLLTLVITVAWKYIKADKFSTLNIIAFMITGYPIAMMWRNASNRAQGAISANLSLL
                     YHRNVGVLDTIFTRVLLEVTGASIAQILLMTIFIAIGWIEMPNDIFYMLCAWILMAFF
                     ALGLGLIICTLAHYIEPFGKIWGTFSFFLLPLSGAFFFVHNLPLTAQNIALWFPMIHG
                     TEMFRHGYFGETVITYESISYLIVSDIILLFIGLLCVRSFSKGIEPR"
     gene            complement(2848..3951)
                     /locus_tag="PXD66_001966"
     CDS             complement(2848..3951)
                     /locus_tag="PXD66_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HDL6320232.1"
                     /translation="MSKKKKHFLANPLLWLTVIIPTACSGIYYSLFASDIYVSESSFV
                     VRSPNNQSSLTGVGALLQNSGFARSQDDTYSVQEYMRSRTALEQLEAEIPVKRFYSEQ
                     GDLLSKFNGLGFNDTQEAFYRYFKERLNIDVDSISGIATLRVRAFNAEEGQKINENLL
                     QQGETLINRLNERARQDTVEFAEERVKEAEKDVNNTALALSQYRTKNKIYDLPAQSSV
                     QLSLISTLKSELIRVETQLAQLKSITPDNPQVAALQMRQKSLKKEIDDQSKQLAGGNT
                     SVATQTAEYQRLVLANELAQQQLAAAMTSLQNTRGEADRQQLYLEVIGRPSKPDWALE
                     PYRIYNIISTFFIGLILFGVLSLLLASIREHKN"
     gene            complement(4023..>5162)
                     /locus_tag="PXD66_001967"
     CDS             complement(4023..>5162)
                     /locus_tag="PXD66_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015702017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HDL6320233.1"
                     /translation="SLISACSSLPTSGPSYSEILASNENTAETQLPEVNLIKLDNITV
                     QNLYQEQQSQRFSGFDGSMGVGGYAGTVNVGDILEISIWEAPPAVLFGGTFSAEGQGN
                     GHLTQLPQQMVNKNGTVTVPFIGNIKVAGKTPEVIQNQIVGALSRKANQPQALVRIAN
                     NLSSDVTVIRQGNSIRMPLSANNERILDAVAAVGGTSENIEDVTVRLTRGNKVRSMAF
                     ETLIADPSQNITLRSGDVIALMNTPYSFTGLGAVGNNQQMRFSSKGITLAEAIGKMGG
                     LIDERSDPRGVFVFRHIPFTQLDYTSQAHWREKGYYEGMDIPTVYQVNLLEPQSIFLL
                     QRFPIQDKDIVYVSNAPLSEFQKFLRMIFSITTPITGTANTIRAY"
BASE COUNT         1632 a          968 c          917 g         1645 t
ORIGIN      
        1 ggctttttaa agcaatgaat acccaaaaaa ttcacgcttt ttgcataagt tatccacaag
       61 gggtatgtca cgctttttta taaaatttat ttttctttat ttatagcgta aacttttcac
      121 atctttattt ttatgcggaa atgtaaaaat gttagaaaaa aatcaccgct tgtccttgcc
      181 aaacaggcaa acgctacgct gaatgttgcc aacctttcca tttaaaacag gcatttgcat
      241 caacggcaga gcaattaatg cgttcccgtt acagtgctta tactttgatt aatattccct
      301 atattgtgga aacgacagtg ccaagtcagc agaaattact tgatcagact gcaatgaaag
      361 agtgggctga aaatacaaaa tgggtaggat tatccgtgct aaatcactta ccttctcttt
      421 cgaaaataca tagtcaggtt gaatttaaag ccttttttga tacagatgag gggatacagg
      481 cacaccacga aacctctctt tttgttcaaa ttgataagcg atggtttttt gttgatccca
      541 ccgtaccttt gcctcatcaa aaacaacctt gtgtatgtgg ttccggaaag aagtttaagc
      601 actgttgtgg aaattatctt taatttgaat tgaactcttg tttgaatcta aggactaatg
      661 agtaaatttc atttacttac caagaggaac aaaaatggat ttcagtaaga ttttaagtca
      721 agtattagat acagcaaaag atgctgctac aaacggatta attaaaggta attctaaaaa
      781 cgatcaaatc gctaaaattg gtggcggcgc tgccgctatt ggtttaattt caatgttatt
      841 tgggcgtaat ggcggttcaa gtttagcgaa attaggctct ttagctgctt taggtagcct
      901 tgcttatcag gcatatcaaa aataccaagc acaaaatgca tcagcatcag agttaagcca
      961 aaatagtttt gagaatgttg ataaaaatgt agcaagtaaa gtgattttac aagcaatgat
     1021 tgccgctgcc gcttcagatg gtgctattac tgaagaggaa aaagcagcta tcatttctca
     1081 agttggagat gatgtagaag taattgagtg gattcgtcaa gaaatggatt ccccagcttc
     1141 agtcgaagag attgctcgtc aagtaggtaa tgatcaagca ttagctactc aagtttactt
     1201 agctgcccgg gcagtatgta ccaatttaga acgcaaagaa atagttttct tagctaatct
     1261 agctgaagct ctagggttag cagaggcttt agtggaacaa ttagaaaaag atgcaggctt
     1321 ttaattttgc ttatttttag taacaagcgg ttatattttg agagtttttt gcgaaaaact
     1381 tgtgaaatat gaccgcttgt tcttttttat gttttattgt aaaaattgta tgcgttatca
     1441 atagaatcaa atgtatacat gataccctct tctaaaacca ttgcagaatc gcaatattct
     1501 ttaattgagg ctgggctatg agaaactaaa atcaatgaac ggtctttccg tttttcaaaa
     1561 agctcatact tgcattttgc tgcaaatcgg gcatcgccca cagcaattac ttcgtcaatt
     1621 aaataacaat caaattcaat agagagagat aaagcaaaag cgagtcttgc tttcatacca
     1681 gaagaatagt ttttgaccgg ctcatatagg tattccccga gttcagaaaa ttcttcggta
     1741 aatgatttta cataatcaaa atctgcacca taaattctac ttacaaaccg taggttatcc
     1801 ataccggtta aacttccttg gaaagcaccg ctaaacgcta ggggccaaga aatagacatt
     1861 tggcgttcta ttgtgcctgt tgatgggggt tctaccccgc tcattaaacg gattaaggtt
     1921 gattttcctg caccattccg ccctaaaatc cccacttttt cgcctttttt cagctcaaaa
     1981 tttatatctt ttaatatggt tttccaaccg ttattagttc gatattgctt acttacattt
     2041 ctaatactaa tcatcttggt tctatccctt tactaaaact tctcacacaa agcagtccta
     2101 taaatagaag aataatatca cttactataa gataactaat gctctcatag gtaattaccg
     2161 tttcaccaaa ataaccatgt ctaaacattt ctgtaccatg aatcattgga aaccataagg
     2221 caatattttg agcagttagc ggtaaattat ggacaaagaa gaatgcacca gaaagaggaa
     2281 gtagaaagaa gctgaatgtt ccccaaatct taccaaaagg ttcaatataa tgtgctaaag
     2341 tacagataat taaacctaaa cctaaggcaa aaaatgccat taatatccaa gcacagagca
     2401 tatagaaaat atcgttaggc atttctatcc aaccaatagc gataaatata gtcattaaga
     2461 gaatttgggc gatagatgcg ccagtaactt ctaataaaac tcgagtaaat atcgtatcta
     2521 gcactcccac atttctgtgg taaagcaaac ttaagtttgc tgatattgct ccttgagctc
     2581 tattagaggc atttctccac atcattgcga tagggtagcc tgtaatcata aaagcgataa
     2641 tatttaaagt tgaaaactta tctgctttta tatatttcca agcaacagta atcactaacg
     2701 ttaacaataa tggttcgata aaaagccata agaagccaat atttttacgc ccataacgag
     2761 taattaattc cctcattaaa agggcgtgaa tcacccgttt ttgaatcaca caggaacgtt
     2821 taaaggttgt attatcacca tatcgcatta gtttttatgc tctctaatac ttgctagtaa
     2881 caaacttaat actccaaata gaattaagcc gataaaaaaa gttgatataa tattataaat
     2941 tctgtagggt tctaatgccc aatctggttt gcttggccta ccaattacct ctaaataaag
     3001 ttgttggcga tcagcttcac ctcgtgtatt ttgtaatgaa gtcattgctg ctgcaagttg
     3061 ttgctgagct aattcgttag caagaaccaa acgttgatat tcagcagttt gcgttgctac
     3121 agaagtatta cctcctgcaa gttgtttgga ctgatcatca atttcttttt tcaaactttt
     3181 ttgacgcatt tgtaatgcgg ctacctgagg attatccggc gtaattgact tcaattgtgc
     3241 taattgtgtt tctacccgaa ttaattcact ttttaaggta gaaattaatg agagctgaac
     3301 gctggattgt gccggtaaat cataaatttt atttttagtt cgatattggc ttaatgctaa
     3361 cgcagtatta ttgacgtctt tttcggcctc ttttacacgc tcttctgcaa actcaaccgt
     3421 atcttgtctt gctctttcat taaggcggtt aattaatgtt tccccttgct gtaataaatt
     3481 ctcgttaatt ttttgtcctt cttctgcatt aaaagcacga acgcgtagcg ttgcaatacc
     3541 tgaaatagaa tcgacatcaa tattcagacg ttctttaaaa taacgataaa atgcttcttg
     3601 ggtatcgtta aatcctaaac cgttaaattt gcttagtaaa tcaccttgct cactataaaa
     3661 acgctttacc ggaatttctg cttcaagttg ttctaatgcg gtacgggaac gcatatattc
     3721 ctgaactgaa taggtatcat cttgagaacg ggcgaaaccg gagttttgca ataaagcacc
     3781 aacgcctgtt aagctcgatt gattattggg cgaacgaacc acaaagctag attctgaaac
     3841 ataaatatca gaggcaaata aggaataata aattccagaa caagcagttg gaataattac
     3901 agttagccac aacaaagggt tagctaaaaa atgttttttc ttctttgaca taaaatcctc
     3961 aacgaaaagt gttacaagcg gtcatttttt aatgattttt taccatttta tctatgagct
     4021 aattagtatg ctctaattgt attagctgta cctgtaattg gcgttgtaat ggagaaaatc
     4081 attctcaaga atttttgaaa ttcagacagt ggagcattcg acacatacac aatatcttta
     4141 tcttgaatag ggaaacgctg taataaaaaa attgattgtg gttctaataa atttacctga
     4201 taaacggttg gaatgtccat tccctcgtaa tagccttttt ctcgccaatg agcttgacta
     4261 gtatagtcta actgggtgaa aggaatatgg cggaatacga acacaccacg agggtctgaa
     4321 cgctcatcaa ttaatcctcc cattttaccg atagcttcag caagggtaat gcctttactt
     4381 gaaaaacgca tttgttgatt attacctact gctcctaatc cggtaaagct ataaggagta
     4441 ttcattaaag caataacatc acctgaacgt aatgtaatat tttgtgaggg gtcagcaatt
     4501 aatgtttcaa acgccattga acgcacttta tttccacgtg ttaaacgaac tgttacatct
     4561 tcaatatttt cgcttgtacc acctactgcc gcaactgcat ctaaaatacg ctcattattg
     4621 gcacttaatg gcatacgaat gctatttcct tggcggataa ccgtaacatc agacgacaag
     4681 ttattcgcaa tacgaactaa tgcttgaggt tgattagctt tacggctcaa tgctccgaca
     4741 atttgattct gaattacttc aggtgttttt ccagcaactt taatattacc gataaaaggc
     4801 acagttaccg ttccattttt gttcaccatt tgctgaggta attgggttaa atgcccatta
     4861 ccttgccctt cagcactaaa tgttccacca aatagcacgg caggtggcgc ttcccaaatg
     4921 gatatttcta atatatcgcc tacattaact gttccagcat aaccacccac acccattgag
     4981 ccatcaaatc ctgaaaaacg ttgagattgt tgctcttggt ataagttttg tacggtgatg
     5041 ttatctaact tgattaaatt gacttcaggc aactgcgttt cagctgtatt ttcattactt
     5101 gctaagattt cgctatagct tgggccggac gttggtaggc tagagcaggc tgaaattaat
     5161 ga
//
LOCUS       DANLXF010000173         5143 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.173, whole genome shotgun sequence.
ACCESSION   DANLXF010000173 DANLXF010000000
VERSION     DANLXF010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5143
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.173"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(35..763)
                     /gene="cmoA"
                     /locus_tag="PXD66_001968"
     CDS             complement(35..763)
                     /gene="cmoA"
                     /locus_tag="PXD66_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HDL6320234.1"
                     /translation="MQRDTLFSAPIEKLGDFTFDESVAEVFPDMIQRSVPGYSNIITA
                     IGMLAERFVTDNSNVYDLGCSRGAGILSIRRNVNAKNVQIIGVDNSQPMVERCKTHLS
                     AYHSEIPVTILLDDIRHIEIKNASMVVLNFTLQFLPREDRLALLQKIYQGLNSNGILV
                     LSEKFTFESEEMNELLIDLHHTFKRANGYSELEVSQKRNALENVMRTDSIETHKKRLQ
                     EAGFTQVELWFQCFNFGSMIAIKA"
     gene            complement(773..1555)
                     /locus_tag="PXD66_001969"
     CDS             complement(773..1555)
                     /locus_tag="PXD66_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL6320235.1"
                     /translation="MTEKSQLNFNYQPSNNHANAQTMIFLHGLFGDLNNLGAIAKPFA
                     ERYSILMVDLRNHGRSFHSDEMNYALIAQDLAQLLTELNIQNAIVVGHSMGGKSAMAL
                     AAFAPELVEKLVVIDIAPVTYTQNRHQQVMAALFAVKEAKPQNRQQAKAIMAEYIRDE
                     GVQQFMLKSFDAQSEQSFRFNLQALKDNYQNVMGWQQVFVDKPTLFIKGALSDYIQAE
                     HTQTILAQFPQAKSFIVANADHWVHAEKTGTVVRAINKFLEK"
     gene            1674..2240
                     /gene="seqA"
                     /locus_tag="PXD66_001970"
     CDS             1674..2240
                     /gene="seqA"
                     /locus_tag="PXD66_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HDL6320236.1"
                     /translation="MKRIEIDDELYQYIASRTQSIGETASDILRRLLRLPQSPQPFVL
                     VQEHMINELKELVKTPSRATKKNESNAEKTVEKLENILNSEHFMNENKNVVRFTMLLA
                     ALYRANPEAFAKATEKVRGNERIYFSQSEAEILATGSGVKAKQIPDSPFWVITNNNTA
                     RKGLILKGVMESMKVPSQLIERIQALFI"
     gene            2250..3668
                     /gene="menE"
                     /locus_tag="PXD66_001971"
     CDS             2250..3668
                     /gene="menE"
                     /locus_tag="PXD66_001971"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HDL6320237.1"
                     /translation="MLINSTRFPTQFWAAHSAQNVAIIWEKGTSDYLNHLPSNITWYS
                     LNQLIAQAIFVLQQQGATPHSLIAYCGSHRLIGLLTYCASLALGAKILMLNPAQTASQ
                     RQAVLDDNGVELIINDADFAKFSAKTTACNAFPTIDFEKPATLTLTSGSSGKPKAVVH
                     SIAAHLYSAEGVCELMAFEQPNSWLLSLPLFHVSGQGIIWRWLFKGATLYVHEDKSDF
                     FLSAQKVSHLSLVPTQLQRYLNQLDSMVSQKCLLGGSMLPAELIAQAQQSGMTTFSGY
                     GMTEMASTICAVENDLNSVGFPLKHRELKLENGEIWVKGKSLGLGYWQKNGQIRPLVN
                     TQGWFATKDRGEWNHKKQLVIKGRLDNMFISGGENIQPEEIEQLIFRSQLVEQIIVLP
                     IEDKQFGHRPVAFLQFKQSDSKNELHKLKIWLSDKLERFKQPVAYYLLDIEKYQKQGL
                     IKLSRTQLQQDLKLKVLKEIYV"
     gene            3661..>5143
                     /locus_tag="PXD66_001972"
     CDS             3661..>5143
                     /locus_tag="PXD66_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HDL6320238.1"
                     /translation="MFRPILKTFVLSLGLMLNSYALAELPTQQVVQTQLDSAKKLDQA
                     DADNKALVQTLEDTLAFLAQIDKQKADNQALNKSIQDSQKALKTSQHNLEKLKEVTIA
                     SVDNLTQKATTDLQKELTSTQSQLETVQHDLSIINNNLVAQNTAPDKAQTALTEMVKR
                     KQEISSQLSATNIKNELQTKLEVELELIELKNTYNQILLGGSEDLSALYSAQLDEKKQ
                     EQQNLQAEIANLQTAINNKIVEESQNKLDQAQAVQNQQNNATTNPVILRELDINTKVT
                     QELLKQTKDMTQLSQDNLRIKSVLDNLQQTQRNIEEQISSLQGTLVLSRIINKQKQSL
                     PQDEMISGLSKQIADLRVRVFDITEFKDSFADINAYISRIEQDEKTTFTSKEKEQLSK
                     ILQERSDTLTEMIKSLNNQLNLLINIELNQQQVQTISDALQQKLQQQSFWVKSNNPID
                     LEWFSKFVDITHYQFIDIGKKLNFSNWRDNLLPASILVGILLLL"
BASE COUNT         1748 a         1008 c          911 g         1476 t
ORIGIN      
        1 aatttgcaaa aaattttaca aatatcaccg cttgttaggc tttaattgca atcattgagc
       61 caaaattaaa gcattgaaac caaagttcaa cttgtgtaaa accagcttct tgcagacgtt
      121 ttttatgggt ttcaatacta tctgttcgca tcacattttc caaagcatta cgcttttggc
      181 tgacttccaa ttcactgtag ccatttgctc gtttgaatgt gtgatgaagg tcgatcaata
      241 gctcgttcat ttcctcactc tcaaaggtaa atttttccga taacactaaa atgccattgg
      301 aatttaatcc ttgataaatc ttctgtaata atgctaggcg atcttctctt ggtaaaaatt
      361 gcaaggtgaa attaagtacc accattgaag catttttaat ttcaatgtgg cgaatatcat
      421 caagtaaaat cgtcaccggg atctcagagt gataagcact taaatgggtt ttacaacgct
      481 caaccatcgg ctgagaatta tctaccccga taatctgcac attcttcgca ttcacattac
      541 ggcgaataga taaaatccct gcgcctcgtg aacaacctaa atcataaaca tttgaattat
      601 ctgtgacaaa acgctccgcc aacattccaa tagcagtaat aatattggaa tagcccggca
      661 cggagcgttg gatcatatcc ggaaacactt cagcaaccga ttcatcaaaa gtaaaatcgc
      721 ccaatttctc aatcggagcg gaaaataatg tatctctttg catttttctc ccttattttt
      781 ctaaaaattt attgattgct ctcacaacag ttcctgtttt ttctgcgtga acccaatgat
      841 cagcgtttgc cacaatgaaa gatttggcct gcggaaattg tgccaaaatc gtttgtgtat
      901 gctctgcttg aatataatcc gataatgcac ctttaataaa caaggtcggt ttatccacaa
      961 atacctgctg ccaccccatc acattctgat aattatcttt tagcgcctgt aaattaaaac
     1021 ggaaagattg ctcgctttgg gcatcaaaag atttcaacat aaactgttgc acgccttcat
     1081 ctcgaatata ttcagccatt atcgctttag cttgctgacg attctggggt ttagcttctt
     1141 tcaccgcaaa taaggctgcc ataacctgct gatgccgatt ttgggtataa gtgaccgggg
     1201 caatatcaat cactaccaat ttctccacca gttcaggagc aaatgcagcc aatgccatcg
     1261 cactttttcc gcccattgaa tgcccaacca caatagcatt ttggatattt aattctgtga
     1321 gtaattgtgc taaatcttgt gcaatcaaag cataattcat ttcgtctgaa tgaaaagatc
     1381 gcccgtgatt tctgagatca accattaaaa tggagtaacg ttctgcgaat ggttttgcta
     1441 ttgcgcctag gttatttaaa tctccaaaca atccatgtaa gaagatcatc gtctgagcat
     1501 tagcatgatt attcgaaggt tggtaattaa agtttaactg cgatttttct gtcatataaa
     1561 tgtcattgtt catataaatt ttcagcgatg ttcctctata aaaaacaata gaaatgcgta
     1621 taatagcaag cactattaac gcatttaata cccgcagata tttgaggatc ccgatgaaac
     1681 gaatcgaaat tgatgatgaa ttatatcagt atattgcaag ccgtacacaa tctattggtg
     1741 aaactgcgtc agatatatta cgccgtctat tacgcctgcc acaatcgcct cagccttttg
     1801 ttttagtaca agaacacatg atcaacgagc ttaaagagct tgtcaaaacg ccttctcgtg
     1861 ctacgaaaaa aaatgaatcc aatgcagaaa aaaccgttga aaaattggaa aacattttaa
     1921 attccgagca ttttatgaac gaaaataaaa atgtagtgcg ttttactatg ctattagccg
     1981 cattatatcg agcaaatccg gaggcttttg ctaaagcaac cgaaaaagtg cgtggtaatg
     2041 aacgtatcta cttttcgcaa agtgaagccg aaattttagc tactgggagt ggagtaaaag
     2101 ccaagcagat cccagattca ccgttttggg taattaccaa taataatacc gcacgcaagg
     2161 gacttattct caaaggcgta atggaatcca tgaaagtacc aagccagtta atcgaacgca
     2221 ttcaagcgct ttttatctaa ttaattgaaa tgctcatcaa ttctactcgc tttcctaccc
     2281 aattttgggc tgctcattca gcccaaaatg tggcgattat ttgggaaaaa ggaacatcag
     2341 actatcttaa ccaccttcca tcaaacatca cttggtatag tttgaatcag ttgattgcgc
     2401 aagctatttt cgtgttgcaa caacaaggtg ctactccgca cagtttgatt gcctattgtg
     2461 gttctcatcg gctcattggc ttgctcactt attgtgctag ccttgctttg ggggctaaga
     2521 ttctaatgct taaccctgct caaacagcaa gtcaacgaca agcagtctta gatgacaatg
     2581 gtgttgagct cattattaat gatgccgact ttgcaaaatt ttcagcaaaa acgaccgctt
     2641 gtaatgcctt ccctacgatt gattttgaaa aaccagctac cctaacccta acctcaggct
     2701 caagcggtaa acctaaagca gtggtgcatt ctatagcggc ccatctctat agtgccgaag
     2761 gagtatgtga attaatggca tttgagcagc caaactcttg gctactgagc ttgcctctgt
     2821 ttcacgtttc cggacaaggc attatttggc gttggttatt taaaggtgca acactgtatg
     2881 ttcacgaaga caaaagtgat ttttttcttt cagcacaaaa agtgagccat ctctctttag
     2941 tccccaccca attacaacgc tatttaaacc aattagattc tatggtcagc caaaaatgtt
     3001 tactgggtgg atctatgctt ccagccgaat tgattgctca agcacaacaa tctgggatga
     3061 ctaccttttc aggctacgga atgactgaga tggcatcaac catttgtgca gtagaaaatg
     3121 atttaaacag tgttggtttt ccgttaaaac atcgggaact aaaattagaa aatggtgaaa
     3181 tttgggtaaa aggcaaatca ctcggcttag gctattggca aaaaaatggg caaattcgac
     3241 cgcttgtaaa cacacaaggc tggtttgcca ctaaagatcg tggcgaatgg aatcacaaaa
     3301 aacagcttgt aataaaagga cgattggata atatgtttat ttcaggaggg gaaaatatcc
     3361 aacctgagga aattgaacaa ctgatttttc gttctcagct tgtcgaacag attatcgtgt
     3421 tgccgattga agataagcaa tttggacatc gaccagtggc gttcttacaa ttcaagcaat
     3481 ctgattcaaa aaatgaactc cataaattaa aaatttggct gtctgataaa ttagaaagat
     3541 tcaaacagcc tgttgcctat tatttactgg atatagaaaa ataccaaaaa caaggcttaa
     3601 ttaaactatc aagaacacaa ttacaacaag atttaaaact caaagtatta aaggaaatct
     3661 atgtttagac cgattttaaa aacatttgtg cttagtttag gcttaatgct aaatagttat
     3721 gcattagctg aattgccaac ccaacaggta gtgcaaacgc agttagatag cgccaaaaaa
     3781 ttagatcaag ccgatgcgga taataaggca ttggttcaaa cacttgaaga tacgcttgct
     3841 ttcttagccc aaattgataa acaaaaagca gataatcagg cattgaataa atctattcaa
     3901 gattcccaaa aagcattaaa aacaagccaa cataatcttg aaaaattaaa agaggtgact
     3961 attgcaagtg tcgataattt aacacaaaaa gcgacaacag atttacaaaa agagttaacc
     4021 tctacacaat ctcagctgga aacagtacaa cacgatttaa gtattattaa taataattta
     4081 gttgcacaaa ataccgcacc ggataaggct caaacggcct taaccgagat ggttaaacgt
     4141 aaacaggaaa ttagtagcca actttcagcg actaacatta aaaatgaact acagacaaag
     4201 ttagaagtag aattagaatt aatagaatta aaaaacactt acaatcaaat attacttggt
     4261 ggcagcgagg atttatctgc gctttactct gctcaattag atgaaaaaaa gcaagagcag
     4321 caaaatctac aagcagaaat tgcaaacctg cagactgcta tcaacaataa aattgtagaa
     4381 gagtctcaga acaagctaga tcaagcacaa gcggttcaaa atcagcaaaa taatgcaacg
     4441 acgaacccag ttatcttgcg tgaattggat ataaatacca aagtcacaca agagttgcta
     4501 aaacagacca aagatatgac gcagttatca caagataatt tgcgaatcaa aagtgtgcta
     4561 gataacttac agcaaaccca acgtaatatt gaagaacaaa tcagttctct gcaaggtacg
     4621 ttggtgcttt ctcgtattat taataagcaa aaacaatcat taccacaaga tgaaatgatt
     4681 tccggactat caaaacaaat tgcagattta cgtgtacgtg tttttgatat cactgaattt
     4741 aaagactctt ttgcggacat aaatgcttat atcagccgca tagagcaaga tgaaaaaaca
     4801 acctttacga gtaaagagaa agaacaactg agtaaaattt tacaggaacg ttctgacact
     4861 ttaactgaga tgattaaatc attaaataat cagctcaatt tattaatcaa tattgaacta
     4921 aaccagcaac aagtgcagac cattagtgat gcgttacagc aaaaactgca acagcaaagc
     4981 ttctgggtta aaagtaacaa ccccattgat cttgaatggt ttagtaaatt tgtcgatatt
     5041 acccactatc aatttattga tattggaaaa aaactcaatt tttcaaattg gcgggataat
     5101 ttattacctg caagtatttt agtcggaatt ttattgctat tag
//
LOCUS       DANLXF010000174         4965 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.174, whole genome shotgun sequence.
ACCESSION   DANLXF010000174 DANLXF010000000
VERSION     DANLXF010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4965
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.174"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..633)
                     /locus_tag="PXD66_001973"
     CDS             complement(<1..633)
                     /locus_tag="PXD66_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HDL6320239.1"
                     /translation="MAEGLLLTPYFQTEVGIVFYKVPKDVSPAAFGERTLLQPAPTDL
                     ATVKSGKVANFSANSTACKELAVFAKSAKVRSTLNQRDKYSNDLPYIRFVKSIQTCQC
                     RDGKYCHTEKVLVENKNGAVQLCWHHDKMRMDGEIPNEKLEALADENWEKFIAQQIRL
                     TLRKTQSTAIEFADLVLFASIKGLLDELDSKALQQFLGYPPLNNSGVSKES"
     gene            complement(760..1353)
                     /locus_tag="PXD66_001974"
     CDS             complement(760..1353)
                     /locus_tag="PXD66_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine methyltransferase"
                     /protein_id="HDL6320240.1"
                     /translation="MTDFDKNTYQTPNYVRNWLNHRYAWFHIDGCSNGQNTLYTYWIG
                     RAADGLDDDKLSCQIADDFLADDLFDVLLDKVSDWGELLRIFVNPPYSDPLPFVQRAA
                     ELKKAGHLVVMLLPADKTTEWYTIIQQHANEVIDIIGYHDEKGTWRTGRIQFINPVTG
                     KPAQGNNKGSMIVVFDPFIEGIVTRQMPLDKIKEWGK"
     gene            complement(1364..2410)
                     /locus_tag="PXD66_001975"
     CDS             complement(1364..2410)
                     /locus_tag="PXD66_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HDL6320241.1"
                     /translation="MRFTSTINNVRLVEWEINITQGALVDLINQASSWAKAVVIDGIT
                     YYWMSFSKVCEELPAVFSKEDTVYRQYKVLKEKGIIDHFKMDGKDYVRLTEKGCEWNK
                     FEPIRESEKNPSIGNNSEQTRKNFRESSEKNPTDNNTNYKNNNDHTTPLNPPAGEPAP
                     AEVVLNYLNSALVTLAVQLGERKPVGYSLKPWAKNITARIRESSVADCCQVVDYLVAK
                     WGRDEKMREYLCPKTIFRQSNFADYFPKSTAWANNGKPVCVNGKWVTPAELEKRLIMP
                     TVDEVRALFQKSLSGNPFKALDFTNKRNLVMYHATINTRNKRPLERDLPMIISQEIKN
                     AVERIDRLRVPTFS"
     gene            complement(2407..3090)
                     /locus_tag="PXD66_001976"
     CDS             complement(2407..3090)
                     /locus_tag="PXD66_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein/antirepressor Ant"
                     /protein_id="HDL6320242.1"
                     /translation="MNQLLNISAPKASITMSSREIAELCEKEHRHVTRDIEAMFSQLD
                     IPAEGYAHFWTHPQNGQTYREFRLPYDLTLTLISGYSVILRKKIIDRWQELESRQGIP
                     QSFSQALRLAAEQQEVIEQQNHQLAIQAPKVAFVEHYVEVGTTKSLRETAKILNFPEK
                     RLIECLERYRVLYRQSGNLLPYQDKQAKQLFAVKTGTAEHGHNFTQTRVTAKGIEWIA
                     QRYASELGQ"
     gene            complement(3148..3336)
                     /locus_tag="PXD66_001977"
     CDS             complement(3148..3336)
                     /locus_tag="PXD66_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDL6320243.1"
                     /translation="MKKDDVLEHFGTLEKVAATLGISVSAVSQWGEIIPEKNAYRLQE
                     ITAGKLKVKHSLYRNRSK"
     gene            3461..4117
                     /locus_tag="PXD66_001978"
     CDS             3461..4117
                     /locus_tag="PXD66_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL6320244.1"
                     /translation="MNTQTLGDRIKSRRKELKLTQKDLATALKGVSHVAVSQWESSTT
                     KPNSENILDLSTVLQCDISWLLRGNGESNVLPASLGMTKVPLISYIQAGQWTGIDDFR
                     ETCGDYEYILTDLEVSEDAFALEIKGDSMEPDFIEGDRVIIDPDVQPHAGEFVAAING
                     DYEATFKKYRPLEDLDEYGRQHFELIALNPDYHKLSTLKQEIRIIGTMVEHRIYRRKR
                     "
     gene            4129..4503
                     /locus_tag="PXD66_001979"
     CDS             4129..4503
                     /locus_tag="PXD66_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320245.1"
                     /translation="MTEFKDVKQAIVEAQLASTPKDKVVNDIMKGSAELLSANDIINR
                     LGIGADEFQELVNLPNHYLRINGGLGAAVYNTRRMFDTHVELVNQELEKNISFPKPDL
                     YILGKARWKKETFKKWLEEQCR"
     gene            4655..>4965
                     /locus_tag="PXD66_001980"
     CDS             4655..>4965
                     /locus_tag="PXD66_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320246.1"
                     /translation="MLSKQVLSISESRLSTYLFCFFRNDPTKQKEAIAIYTALQHRTG
                     IYFSLIQEIEVALRNEVSQLLRFAAPNHNLYEFFHFLANDSNAPLNAESKRQLQKAIS
                     E"
BASE COUNT         1436 a         1024 c          961 g         1544 t
ORIGIN      
        1 actttctttg ctcacaccgc tattattcag tggtggatag ccgagaaact gctgtaatgc
       61 cttgctgtct aattcatcaa gcaaaccttt gatactcgca aacaacacta agtccgcaaa
      121 ttcaatagcg gtagattgcg ttttgcgtaa ggttaagcgg atttgttggg cgataaattt
      181 ttcccagttt tcatccgcta gtgcttctaa tttttcattc gggatttcac cgtccattcg
      241 cattttgtcg tgatgccaac aaagctgtac cgcaccgttt ttattctcta ccagcacttt
      301 ttccgtgtgg caatatttgc catctcggca ttggcacgtc tgaattgact ttacaaaccg
      361 gatataaggc aaatcattac tatatttgtc acgctgattt agcgttgaac gaacttttgc
      421 cgatttagca aacaccgcca gctctttaca agcggtcgaa tttgccgaaa aatttgcaac
      481 cttgcctgat ttaaccgtcg ctaaatcggt cggtgcaggt tgcaacaaag ttcgctcacc
      541 gaaagcagca ggagaaacat ctttaggcac tttgtaaaac acaataccca cttcggtttg
      601 aaagtaaggg gtaagtagca aaccttccgc cattattgcc cccatttctc ggtttcatca
      661 taggctttca cgaatagcat aaatacgcca atacagatta aaaaaatcag tacgccaatt
      721 actgcaccaa taccaataaa tagttcccaa taatccattt tatttacccc attccttgat
      781 tttatctagt ggcatctgac gagttacaat gccctcaata aatgggtcaa acacaacaat
      841 catcgagcct ttattattac cttgtgccgg tttacctgtt accgggttga taaattgaat
      901 ccgccctgtc cgccaagtgc ctttttcatc gtgatagcca ataatatcaa ttacctcatt
      961 agcgtgttgt tgaatgatgg tgtaccattc tgttgtttta tccgctggta ataacatcac
     1021 aaccaaatga cctgcttttt tcaattctgc cgctcgttga acaaaaggca gtggatcaga
     1081 atatggcgga ttaacgaaaa tacgcagtaa ttctccccag tctgaaactt tatctagtag
     1141 cacatcaaat aaatcatcgg ctaaaaaatc atcggcaatt tggcaactta atttatcatc
     1201 atctaaaccg tctgccgctc taccaatcca ataggtgtac aacgtatttt gtccgttaga
     1261 gcaaccgtca atatgaaacc aagcataacg atgatttagc caattacgca cataattagg
     1321 tgtttggtaa gtgtttttat caaaatctgt cattgtctaa tccttacgaa aatgttggta
     1381 ctctcaaacg gtcgatacgc tcaaccgcat ttttaatttc ttggctgata atcatcggca
     1441 aatcacgttc aagtggtcgc ttgtttctgg tgttgattgt ggcgtggtac atcactaaat
     1501 ttcgtttgtt ggtaaagtcc aatgccttaa acggattgcc actgagtgat ttctggaaca
     1561 aggctcgtac ttcgtccacc gttggcataa tcaagcgttt ttccagctcc gccggtgtaa
     1621 cccacttacc atttacgcaa acaggctttc cgttattagc ccaagcggtc gattttggaa
     1681 aataatctgc aaaattcgat tggcggaaaa tcgttttcgg gcagaggtat tcacgcattt
     1741 tttcatctcg tccccattta gcgacaaggt aatccaccac ttggcaacag tccgccaccg
     1801 aactttcacg aatgcgagca gtaatatttt ttgcccacgg cttgagcgaa taccccacag
     1861 gcttacgctc accaagctgt accgccaacg ttaccaaagc cgaattgaga taattcaaca
     1921 caacttcagc cggggcaggt tcccccgctg ggggattaag gggggtagta tgatcattat
     1981 tatttttata attagtatta ttatctgtcg gatttttttc cgagctttct cggaaatttt
     2041 tccgagtttg ttcggagtta tttccgatac tcggattttt ttccgactca cggatcggct
     2101 cgaatttatt ccattcacaa cctttttcgg ttaaacggac ataatcttta ccgtccattt
     2161 tgaagtggtc gataatgcct ttttctttca acaccttata ttggcggtaa actgtatctt
     2221 ctttgctaaa aaccgcaggt aactcttcgc aaaccttgct aaatgacatc cagtaatagg
     2281 ttatgccgtc aataaccacc gctttcgccc aactggaggc ttgattgatt aaatcgacca
     2341 atgccccttg tgtaatattg atttcccatt ccactaagcg gacattgttt atggttgatg
     2401 taaatctcat tgtcctaact ccgatgcgta acgttgagca atccattcaa tccctttggc
     2461 ggttactctc gtttgggtaa aattatgacc gtgttctgcc gtgccggttt ttactgcaaa
     2521 aagctgttta gcttgtttat cttgataagg gagtaggttt cctgattggc gataaagaac
     2581 tcgatatcgt tctaaacatt caatcaaccg cttttctgga aaattgagta tcttggcggt
     2641 ttctcgcaat gatttagtcg ttcccacctc aacataatgc tccacaaatg cgactttcgg
     2701 ggcttgaatg gctaattgat gattctgctg ctcaatcact tcttgctgtt ctgccgccaa
     2761 ccgtagagct tgagagaaag attgtggaat accttgtctg ctttctagct cttgccaacg
     2821 gtcgataatt ttcttgcgaa gtatcacgct atagccggaa attagggtaa gggttaaatc
     2881 gtaaggcaat cgaaattccc ggtatgtttg cccattttga gggtgtgtcc aaaaatgggc
     2941 atacccctcg gcaggaatat ctaattgact aaacatcgct tctatatcac gagtaacgtg
     3001 tcgatgttct ttttcgcaca actcagcaat ctcacgacta ctcatcgtaa tgcttgcttt
     3061 tggtgccgaa atgtttaata attgattcat cgaaaatacc tttcgtgttt aattatgtat
     3121 gaaagccacc gttacagcgg tggtttttta tttgcttcta tttctataaa gtgagtgttt
     3181 gacctttaac ttgccggcag tgatctcttg taatctgtac gcatttttct caggaataat
     3241 ctcgccccat tgagaaacag cagatacact aatccctaaa gttgccgcca ctttttctag
     3301 agttccgaaa tgttcgagca catcatcttt tttcatattt tcttaattcc tttcttgaat
     3361 tttatttaag acctcttaaa tatacaatta aaggaaactt aagtcaataa atgttaagat
     3421 aacttaacag caactttatt tttaccttta aggggatact atgaatactc agaccttggg
     3481 cgatagaatt aaaagccgta gaaaagaatt aaaattgaca cagaaagatc ttgctactgc
     3541 gttaaaaggg gtctcacacg ttgcagtatc tcaatgggaa agtagtacga ctaagccaaa
     3601 ttcagagaat attttggatt tatctacagt attgcaatgc gatattagtt ggctcttgcg
     3661 tggtaatgga gagtctaatg ttctacctgc tagcctcggt atgactaaag tcccattaat
     3721 tagctatatt caagccggac aatggacggg aattgacgat tttcgtgaaa cctgtgggga
     3781 ttatgaatat atcctaactg atcttgaggt gtctgaagat gcttttgccc tagaaatcaa
     3841 aggggactcg atggagcctg attttattga aggagaccga gtaattattg atcctgatgt
     3901 acaacctcac gccggtgagt ttgtggcagc aattaatggc gattatgaag ccactttcaa
     3961 aaaataccgt ccgttagaag acttagacga atacggaaga caacattttg agcttatcgc
     4021 tcttaatccg gattaccata aattatcaac gctaaagcaa gaaatcagaa ttatcggcac
     4081 aatggtagag catcgtattt atcgtagaaa gagataggaa ttgataatat gacagaattt
     4141 aaagatgtaa aacaggcgat tgtagaagct caattagctt cgacacctaa agataaagtc
     4201 gttaatgaca taatgaaagg aagtgcggaa ctgctttcag caaatgatat tatcaatagg
     4261 ttaggtattg gggcagatga gtttcaagag ttggttaatt tacctaacca ttatttgcgt
     4321 ataaatggag ggttaggtgc tgctgtgtat aatacaagaa gaatgtttga tacccatgtt
     4381 gagttagtca atcaagagtt agaaaagaat atttcatttc ctaaacctga tttatatatt
     4441 ttggggaaag ctcgttggaa aaaagaaaca tttaagaaat ggttggaaga acagtgtaga
     4501 taaagaaaag gtaggttaaa aaacctacct tttctgaacc gcaaataagc gattctcaca
     4561 ctttacaaag caatccagct cctacgtctg gggcttcggg tccgttgcca gtgcaagtat
     4621 tattacatta aactaaactt gagtaaagga aaatatgttg tccaaacaag tgctaagtat
     4681 ttcagaaagt cggctgagta catatttatt ttgctttttc cgaaatgatc ccacaaaaca
     4741 gaaagaagct attgcaattt ataccgcctt gcaacatcga acaggaattt atttttcatt
     4801 gattcaagaa atagaggtgg cactaagaaa tgaagtcagc cagttgttac gctttgctgc
     4861 acctaatcat aatttatacg aattctttca ttttctagcg aacgatagta atgctcctct
     4921 taatgcagaa tctaaacgcc agctacaaaa agccattagt gaatg
//
LOCUS       DANLXF010000175         4945 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.175, whole genome shotgun sequence.
ACCESSION   DANLXF010000175 DANLXF010000000
VERSION     DANLXF010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4945
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.175"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            123..350
                     /locus_tag="PXD66_001981"
                     /pseudo
     CDS             123..350
                     /locus_tag="PXD66_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
     gene            375..770
                     /locus_tag="PXD66_001982"
     CDS             375..770
                     /locus_tag="PXD66_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6320247.1"
                     /translation="MKIHTQIRKEILSLLEAKLTDIEHFYNGQPNFIDIDEQQLAISV
                     YLDEINRQELTLCDEQWTAQLNITIYLKSVDEAEDELDEWAEKIREVVETYSAFEHLE
                     GISLSQYQYEQDQNQRTWHSATLIFDVEY"
     gene            802..1443
                     /locus_tag="PXD66_001983"
     CDS             802..1443
                     /locus_tag="PXD66_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320248.1"
                     /translation="MAKTTKVQGTKFRIGIGREAQKAITAITLATATLTIATSGYKKG
                     DAIEITGCGQLDGIYPVLSVTGDQVKLCEEVNWTGKDLPANYTKAKAALVQYSDQFCA
                     VKNIEKSDDTLSTEDVTTVCSEGTETEPGEIEFGSIKLSFFHKPSTEMQTRLRKLFYD
                     KSTFAYKLELPDNHGTTYGEGFIEAGNGFSGEVKGKYEGSVSIKPSKRDYLLV"
     gene            1526..1927
                     /locus_tag="PXD66_001984"
     CDS             1526..1927
                     /locus_tag="PXD66_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320249.1"
                     /translation="MTLREKLLANKPKLQSIEINGETYYLREATVGDMNKQIFETRSW
                     LIQQAEQENVELPAEDDETFDEALNRFGEKYRLAQSVAYRLCDENGALLFNPLNIDDL
                     NAIAELDSKVIIDFNQAVSAPKDSASEESSK"
     gene            1972..2202
                     /locus_tag="PXD66_001985"
     CDS             1972..2202
                     /locus_tag="PXD66_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HDL6320250.1"
                     /translation="MPERHFAEYQLFYQEQPFGLWREDYRAAQLAHLTAMINRDPKGK
                     APELSEFMPFFNRADEVEANEDDGVADYLAKR"
     gene            2270..2536
                     /locus_tag="PXD66_001986"
     CDS             2270..2536
                     /locus_tag="PXD66_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDL6320251.1"
                     /translation="MATINDAFSFRTNTEIKNTAFDVIKNYGMTPSQVFNMFLTEIAK
                     TKTIPLSLNYQPNLETKLAMQEAKSGKNEVYASLEAFHKAMLAE"
     gene            2540..2809
                     /locus_tag="PXD66_001987"
     CDS             2540..2809
                     /locus_tag="PXD66_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HDL6320252.1"
                     /translation="MLQISPTNAYKRDFKKIAAELVGSSEYVEVMYCLINQLPLAEKY
                     RDHPLQGEWQGFRDCHIKPDLVLIYAVEDNLLRLVRLGSHAELFG"
     gene            2884..3147
                     /locus_tag="PXD66_001988"
     CDS             2884..3147
                     /locus_tag="PXD66_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320253.1"
                     /translation="MKNVLLFIVHFFLIAMLSIIPILIILGAIERDPYYTAWIFLSVF
                     LGIGALLYFATIVSKLINKGKTQTLEEKRYAEYWNNVKVNIPK"
     gene            3207..3917
                     /locus_tag="PXD66_001989"
     CDS             3207..3917
                     /locus_tag="PXD66_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HDL6320254.1"
                     /translation="MSGLGKLTVTLELENAKFQSAMTKSDYEAQKFAKNFIQNMDRAR
                     NHAKEFADRSTQYLKNIEQAAKNLNKNSEFSFLSTIGGHYQSMSSGILSAAKAYTDIQ
                     NKMKLVSGSSAEAAKRLTDVFDIATKTSQSTEAVSGVYQTFAQNAQSLGLAQKDVAEL
                     TKTVSQAVAASGASSSAASNALTQFGQSLLMGKMKAQEFNSLITQTPTIIQAMAKGLG
                     MTMAEFKAAVYRAKSLLG"
     gene            3895..4185
                     /locus_tag="PXD66_001990"
                     /pseudo
     CDS             3895..4185
                     /locus_tag="PXD66_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484718.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            4175..4894
                     /locus_tag="PXD66_001991"
     CDS             4175..4894
                     /locus_tag="PXD66_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDL6320255.1"
                     /translation="MKFSKYQLLVNKICFICGKKDCPQMKKSKDYKDFLDALEKGDVD
                     KADKIYNTKFSQFSKSFGHEMEKNLEKNRIPPIYQGVPFDSFDLYQHYSYGNGEALNL
                     SSVGLSNKIRPLVNKDKAFGKSNGSIESRFISQYKNEGRTSFSNAYDFTKEASGIADP
                     LWALGTATISGELKDIQIFPGGIARGNIHYSIRDRFTDPFDTFNFTKGEWNPNGTPYI
                     IQDSWIKSVRFSIDKKIKITP"
BASE COUNT         1703 a          908 c          973 g         1361 t
ORIGIN      
        1 gaacaagttc tacaagcggt tgtcaagtgt tcatttgttc aatgaacagt tcattgtgaa
       61 taacaaaacc gcttgaatta caagcggtct attttgttgc gtaaatttac tgttagcgtt
      121 gagccataac cgactacgac cgctggcccg aagatattga cggcgagggg gacggcttct
      181 cgcttgcctc caaacggacg accactttta tgtcagccgg aatgacttta gtggagagtt
      241 cgcctggtaa agatattgtc gatctcaaac atatcccgaa aagtagccac gaagccccac
      301 cgactaccgg tattttaagc ctctacaatc gaggcgaccg cagacgcttt agcgaaacag
      361 ttgataggta agctatgaaa atccataccc aaatccgaaa agaaatcctt tcattattag
      421 aggcaaaatt aacggacatc gagcattttt ataacgggca accgaatttc attgatattg
      481 acgaacagca acttgccatc tctgtttatc ttgatgagat taaccgtcag gagcttacct
      541 tatgcgatga acagtggaca gctcaactta acatcacgat ttatctaaaa tcagttgatg
      601 aggcagaaga tgaattagat gagtgggccg aaaaaatccg tgaagtggtt gaaacatatt
      661 ctgcgtttga acatttagag ggcatcagcc tttctcaata tcaatacgaa caagatcaaa
      721 atcaaagaac ttggcactca gccacattga tttttgatgt tgaatactaa caagcaaata
      781 atccttatag gagaatctac aatggcaaaa acaacaaaag tccaaggcac aaaatttaga
      841 attggcattg gacgtgaagc ccaaaaggca attacggcta tcactttagc tacagcaacg
      901 cttactattg ccacatcagg ctataaaaaa ggcgatgcaa tcgaaattac cggttgtggt
      961 cagttagacg gcatttatcc tgtattatct gtaacaggcg atcaagtcaa attgtgcgaa
     1021 gaagtaaact ggacgggtaa agatttaccg gctaattaca ccaaagccaa agcagcttta
     1081 gtacaatact cagaccaatt ctgtgcggta aaaaacattg aaaaatctga tgatacgtta
     1141 agtaccgaag atgttacaac ggtctgctcg gaaggtacag aaaccgaacc cggagaaatt
     1201 gaatttggct caattaaact gagctttttt cacaagccaa gtacggaaat gcaaactcgc
     1261 ttacgcaaat tgttttatga caaatccacc tttgcgtaca aattagaatt acctgataac
     1321 cacggcacaa cctatggcga aggtttcatt gaggcgggta acggctttag tggcgaagtt
     1381 aaaggcaaat atgaaggctc ggtatcaatt aaaccaagca aacgtgatta tttgctagtg
     1441 taattacaaa atattgctcc aaatcgaccg cttgtaatat tgcaagcggt cttttttatc
     1501 ctaaatttta caaaaggaat cgacaatgac actccgtgaa aaactcctcg ccaacaaacc
     1561 aaaattacaa tctatcgaaa tcaacggtga gacctactac ctgcgtgaag ccactgttgg
     1621 cgatatgaat aagcagattt ttgaaacccg aagctggctc attcaacaag ctgaacaaga
     1681 aaatgttgaa ttaccggcag aagatgatga aacctttgac gaagctctca accgttttgg
     1741 cgaaaaatac cgccttgctc aatcggttgc ctaccgttta tgtgacgaaa acggtgcatt
     1801 actgtttaac ccacttaaca ttgacgatct caatgcgatt gccgaattag acagtaaagt
     1861 gattatcgac tttaaccaag ccgtgtccgc cccaaaagac tcagcgagcg aagaaagttc
     1921 caaataaccc tttcgctcgc actgggtaaa acccttgaag aaatcgaaca aatgcctgaa
     1981 cgccactttg ccgaatatca gcttttttat caagagcaac cttttgggct ttggcgtgag
     2041 gactatcgtg ctgctcaact tgcccactta accgcaatga tcaaccgtga cccaaaaggc
     2101 aaagcccctg aactctcgga atttatgccg ttttttaatc gggctgatga agtagaagca
     2161 aatgaagatg atggcgttgc ggattatttg gcgaaaaggt aaatctcttg tatttcattg
     2221 taattctgtc tataattggt gtaatacata gaaatacagg agagataaaa tggcgactat
     2281 caatgatgct ttcagtttta gaacaaatac cgaaataaaa aataccgcat ttgatgtaat
     2341 taaaaactat ggaatgacac cctctcaggt gtttaatatg tttttaaccg agattgcaaa
     2401 aacgaaaact attccgttaa gtttgaatta tcaacccaat cttgaaacaa aattggcaat
     2461 gcaagaagca aaatcaggta aaaatgaagt ttatgcctca cttgaagcat ttcataaagc
     2521 aatgttagcg gagtaaataa tgctacaaat ttcgccgaca aacgcatata aaagagactt
     2581 taaaaagatt gcagccgaat tagtcggtag ttcggaatat gtggaagtaa tgtattgcct
     2641 aataaaccaa ttaccattgg cggaaaaata tagagatcac ccactacaag gtgaatggca
     2701 aggctttaga gattgccata ttaagcctga tttagtgttg atttacgctg ttgaagataa
     2761 tctgctccgc cttgttcgtt taggatcgca cgctgaatta tttggataat ttctgtaaaa
     2821 actaaccgct tgcgattttg tgctgtgggc ggtatagttg gaatattaac aaaggaggga
     2881 aaaatgaaaa atgtacttct atttatcgta cactttttct taatcgcaat gctctcaatt
     2941 atcccaatat taattattct tggagcaatc gaaagagatc cttactatac tgcgtggatt
     3001 ttccttagcg tatttttagg cattggagca ttgttatatt ttgcaaccat tgtttctaag
     3061 ttaattaaca agggaaaaac acaaacatta gaagaaaaac gatatgcaga atattggaat
     3121 aatgtaaaag tcaatattcc caaatagtct aactgataaa tgaccaaaag ctcgctttat
     3181 gcgggctttt ttattggaga aaaacaatga gtggcttagg aaaattaacc gttaccttag
     3241 agcttgaaaa tgcaaaattt cagtccgcaa tgacgaaatc tgattatgag gcacaaaaat
     3301 ttgccaagaa ctttatccaa aatatggata gagctagaaa tcacgccaaa gaatttgccg
     3361 atcgctcaac ccaatattta aaaaacattg agcaagcggc aaagaacctc aataaaaatt
     3421 ctgaattttc atttttatcg actattggtg gacattatca atcaatgtct agtggcatat
     3481 tgagtgcggc aaaagcctat actgatattc aaaataaaat gaaattagta agcggtagtt
     3541 cagcagaggc agctaaacga ttaactgatg tgtttgatat tgcgactaaa acaagccaaa
     3601 gtacagaggc tgtttcaggt gtctatcaaa catttgctca aaatgcccaa tctctcggac
     3661 tggctcaaaa agatgtagca gaattaacta aaacggtctc tcaagccgtt gctgcaagcg
     3721 gcgcaagcag ttctgcagca agtaatgccc taacccaatt tggtcaatca cttttaatgg
     3781 gcaaaatgaa agcccaagaa tttaactcgc tcattactca aaccccaacc attattcaag
     3841 caatggcaaa aggtttggga atgacaatgg cagaatttaa agcagcggta taccgggcaa
     3901 aatctttact tggataacat tcaagggcaa ggttttgaga gagtggatac accccaaatc
     3961 ggcgatgtga ttttaataca ggtcggttct gatgtgccga atcacgcagc gatttatatt
     4021 ggtgaacaaa tggtaatcca ccatagcccg aaccgattat ctaagcgtga tttatatgac
     4081 ggttattggt tacgccatac ccacagtatt tggcggcata aactggcgga tagattggat
     4141 tttgacggca tatttaatga tataggagaa caatatgaaa tttagtaaat atcaattatt
     4201 agttaataag atctgcttta tttgtggtaa aaaagattgt ccacaaatga aaaagagtaa
     4261 agactacaaa gactttttgg atgctttaga aaaaggggat gttgataaag cagataaaat
     4321 atacaatacg aagttttcac agttttctaa atcttttggt catgaaatgg aaaagaattt
     4381 agagaaaaat cgtatacctc ctatttatca gggagttcca tttgattctt ttgacttata
     4441 tcagcattat tcttatggta atggagaggc attaaatctt tcatccgttg gattaagtaa
     4501 taaaattcgt cctctagtga ataaagataa agcatttggt aaatccaatg gaagtataga
     4561 aagccgattt atttcgcaat ataaaaatga aggacgcaca tctttctcca atgcttatga
     4621 ttttacaaaa gaagcatcgg gtattgcaga tccgttatgg gcgttaggaa cagctaccat
     4681 tagtggagaa ttgaaagata tacaaatttt tcccggtggt attgctaggg gaaatatcca
     4741 ttatagtatc agggacagat tcactgaccc atttgataca ttcaatttta ctaaaggaga
     4801 atggaaccca aatggaacac cttatattat tcaagatagc tggataaaat cggtacgatt
     4861 ttcgatagac aagaagataa aaataacacc ttaattatga tagtaatata aaacaattgg
     4921 caatattatt ttaaataaaa aaata
//
LOCUS       DANLXF010000176         4931 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.176, whole genome shotgun sequence.
ACCESSION   DANLXF010000176 DANLXF010000000
VERSION     DANLXF010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.176"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            81..566
                     /locus_tag="PXD66_001992"
     CDS             81..566
                     /locus_tag="PXD66_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HDL6320256.1"
                     /translation="MKIKTLSLIMLLSVTVNAAPVELHQFDSPKQESDYRALIQELRC
                     PQCQNNNIADSNATIATDMRAKTLELLKQGKSKDEVVDYMIERYGNFVTYDPPLTPAT
                     IMLWLLPLILIALGLGVVLRRKKNKPQAVNFEQNTAKPTTHLAQDEQERLNQILNKEE
                     K"
     gene            563..1474
                     /gene="ccmI"
                     /locus_tag="PXD66_001993"
     CDS             563..1474
                     /gene="ccmI"
                     /locus_tag="PXD66_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HDL6320257.1"
                     /translation="MNFWLAIIFVTLIVCGVAFYPLLKKNKQIDNTKRDSLNKAFYFD
                     RLKEVENEANEGVIDDPEQTKLELQKNLLDDIPHSTQSATSEKLTLGKSWFVALILFV
                     GGISFAIYSNVGSWFSNSMLDTTHQKLDSFYERLKTEETNPLSQEEMNQFAMALRVDL
                     QSNPNDDKKWYMLGQIGMALDDGQLAHDAFSKAAQLKPDNLDYKLSHAQLLLFSEAQE
                     DKKQGETLLKEIIRQDHTNFNALSLLAFHSFEKEDFKMAAMTWGMMLKLLPDEDPSKV
                     TVERSFNMALSNLSEAEKQELQKGEKK"
     gene            1471..2076
                     /gene="mog"
                     /locus_tag="PXD66_001994"
     CDS             1471..2076
                     /gene="mog"
                     /locus_tag="PXD66_001994"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HDL6320258.1"
                     /translation="MTACKPLSKEKLKIGLVSVSDRASQGVYSDQGIPELRAWLESAL
                     TEPFELETRLIPDEQAIIEQTLIELVDAHACHLVLTTGGTGPAKRDVTPDATLAVAHR
                     EMPGFGEQMRQVSLHFVPTAILSRQVGVIRHDSLILNLPGQPKAIKETLEGVKDEQGK
                     VLVKGIFSAVPYCLQLLSEIYVETHAAICESFRPKSARREN"
     gene            2086..2424
                     /gene="glnB"
                     /locus_tag="PXD66_001995"
     CDS             2086..2424
                     /gene="glnB"
                     /locus_tag="PXD66_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HDL6320259.1"
                     /translation="MKKIEAIIKPFKLDDVREALTDVGVSGMSITEIKGFGRQKGHTE
                     LYRGAEYAIDFLPKVKVEIVIPDELEEQCIEAIMETAQTGKIGDGKIFVYDVGRVIRI
                     RTGEENEEAI"
     gene            2505..2984
                     /gene="lspA"
                     /locus_tag="PXD66_001996"
     CDS             2505..2984
                     /gene="lspA"
                     /locus_tag="PXD66_001996"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005724521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDL6320260.1"
                     /translation="MKTGLKFLWVSLIVIIIDLWSKYIVVQHFEFGESINILPIFNFT
                     YARNYGAAFSFLADHSGWQKYFFLGLAVVISSALVVALWKNQAVKKLENIAYALIIGG
                     AIGNAIDRAYNGYVVDFLHFYWDIYHYPVFNIADIAICVGAGLLILEAFITKKEPKK"
     gene            2995..3939
                     /gene="ispH"
                     /locus_tag="PXD66_001997"
                     /gene_synonym="lytB"
     CDS             2995..3939
                     /gene="ispH"
                     /locus_tag="PXD66_001997"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HDL6320261.1"
                     /translation="MKILLANPRGFCAGVDRAISIVELALEIHGAPIYVRHEVVHNKF
                     VVDGLKAKGAIFVEELHEVPDDAIVIFSAHGVSQVVRQEAKNRGLKVFDATCPLVTKV
                     HMQVARASKKGTKAILIGHEGHPEVIGTMGQYNNQEGGIYLVESVEDIANLPVSNNDE
                     LTFMTQTTLSIDDTIGVIEALKEKYPAIQGPRKNDICYATTNRQHAVRELAEQSQLVL
                     VVGSKNSSNSNRLAELASRMGVMSKLIDGPQDIDPTWLEGIETIGITAGASAPEVLVQ
                     SVISHLKTLGVTEVETLVGNEEDVVFEVPKELRIKEVK"
     gene            3939..4187
                     /locus_tag="PXD66_001998"
     CDS             3939..4187
                     /locus_tag="PXD66_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HDL6320262.1"
                     /translation="MTEINRFKLEWACRRGMRELDKMIMPFYQNHFNELNETQQQAFV
                     EMLNYSDPELFRWVMLQAPAPTEEIAELIRLMRDKIET"
     gene            complement(4394..>4931)
                     /locus_tag="PXD66_001999"
     CDS             complement(4394..>4931)
                     /locus_tag="PXD66_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL6320263.1"
                     /translation="HSNHSNQSANQYIFNSDWFTHNVPSLASIFEYVKPRRILEIGSF
                     EGRSSVFFIEEALKYQSQVEIHCIDSWEGGAEHQGVWDMGEIEQRFIHNVTLAQQKFQ
                     NVVLYKHRGYSHQKMIELLANGYINYFDYIYIDGSHEAPDVLFDALLAHRLVRKGGVI
                     SFDDYLWSPDETGKQRHY"
BASE COUNT         1568 a          988 c         1058 g         1317 t
ORIGIN      
        1 aaacacagaa acttttgtac tggaaattga aaataacaaa tatcaaattc ctaataaata
       61 tatcaacaat attcaaaatt atgaaaataa aaactctcag cctaataatg cttctaagtg
      121 taacagttaa tgctgctccg gtagaactgc accaattcga cagcccgaaa caagagtcgg
      181 actatcgtgc cttaattcaa gaactgcgtt gcccgcagtg tcaaaataat aatatcgccg
      241 attccaatgc gactattgcc accgatatgc gggcaaaaac cttagaattg ctcaaacaag
      301 gcaaaagcaa agacgaagtg gtggattata tgattgaacg ctacggcaat ttcgtcactt
      361 acgacccgcc tctcacaccg gcaaccatta tgttatggct gttgccgtta attttaattg
      421 ccttagggct gggggtggtg ttgcgtagaa agaaaaacaa accacaagcg gtcaattttg
      481 agcaaaatac tgcaaaaccg accactcatc tcgctcaaga tgagcaagaa cgtcttaacc
      541 aaattctcaa taaggaagaa aaatgaattt ctggctggca attattttcg ttacactgat
      601 tgtatgtggc gtggcgtttt atccgttact taaaaagaac aagcaaatcg ataacaccaa
      661 gcgagacagc ttaaacaaag ccttctattt tgaccgctta aaagaagtgg aaaacgaagc
      721 gaatgaaggg gtgattgatg accctgaaca aaccaaatta gagctacaaa aaaacttgct
      781 tgatgatatt ccacacagca cacaaagtgc cacaagtgaa aaattaacgc tcggcaagtc
      841 gtggtttgtg gcattgattt tatttgtcgg cggtatcagc tttgcgattt acagcaatgt
      901 cggttcttgg ttctccaact caatgttaga caccactcac caaaaattag acagcttcta
      961 tgagcgttta aaaaccgaag agaccaatcc gctctcacaa gaggaaatga accaatttgc
     1021 gatggcatta cgggtagatt tacaaagtaa tccgaatgac gacaaaaaat ggtatatgct
     1081 cggtcaaatc gggatggcgt tagatgatgg tcagcttgca cacgatgcct ttagcaaagc
     1141 ggctcaactc aagccggata acttagatta caaactcagc cacgcccaac tgctgctctt
     1201 ttccgaagca caggaagaca aaaaacaggg tgaaaccttg ttaaaagaga ttatccgcca
     1261 agaccacaca aactttaatg cgttaagttt attggcgttc cactcctttg aaaaagaaga
     1321 tttcaaaatg gcggcaatga cgtggggaat gatgttaaaa ctactgcctg atgaagatcc
     1381 tagcaaagta acggtagaac gcagttttaa tatggcgtta tccaatttaa gtgaggcgga
     1441 aaaacaagaa ttgcaaaaag gtgagaaaaa atgaccgctt gtaaaccact tagcaaagag
     1501 aagttgaaaa tcggcttggt gtcggtttca gaccgagcct cgcagggggt ttattcagac
     1561 caaggcattc ccgaattaag ggcgtggctt gaatctgcct taaccgagcc gtttgagctg
     1621 gaaacccgct taattcccga tgagcaagcc attattgagc aaaccttaat cgagttagtc
     1681 gatgctcacg cttgccattt ggtattaacc accggcggca ccggcccggc aaaacgtgat
     1741 gtcaccccag atgccaccct tgctgtcgcc caccgagaaa tgcccggctt tggcgaacag
     1801 atgcgacaag tgagccttca cttcgtgcca accgccattc tctctcgcca agtgggagta
     1861 attcgccacg acagtctgat tttaaatctg ccggggcaac cgaaagcaat taaagaaacg
     1921 ctggaaggtg tgaaagatga acagggcaaa gtattggtta aaggtatttt tagtgccgtg
     1981 ccatactgtt tacaactttt aagcgaaatt tatgtcgaaa cccacgcagc tatttgcgaa
     2041 agtttcagac ccaaatcggc aagacgagaa aattaaggct aaaaaatgaa aaaaatagaa
     2101 gcaattatta aaccttttaa attagacgat gtgcgtgaag ccttaaccga tgtgggtgtc
     2161 agcggaatga gtattaccga aatcaaaggg tttggcagac aaaaaggaca taccgagctt
     2221 tatcgtgggg cggaatatgc gattgatttt ctaccgaaag tgaaagtcga aatcgtgatt
     2281 cccgacgagt tggaagaaca gtgcattgaa gcaattatgg aaaccgccca aaccggcaaa
     2341 atcggcgatg gcaagatttt tgtgtatgat gtcgggcggg tgatccgcat tcgtaccggc
     2401 gaagaaaatg aagaagcgat ttagttttgc aaaaaatccg gcaaaaacga ccgcttgcag
     2461 ggggatttcc atctactctt tttacattaa attttggata ttaaatgaaa acaggcttaa
     2521 aatttctttg ggtgagttta attgtaatca tcattgacct ttggtcaaaa tacattgtag
     2581 tgcaacattt tgaatttggt gaaagcatta atattttgcc gatttttaac ttcacttacg
     2641 cccgaaacta cggggctgcc tttagtttcc ttgccgacca ttccggctgg caaaaatatt
     2701 tctttttagg gctggcggta gtgatttcca gtgctttagt ggtggctctt tggaagaatc
     2761 aagcggtcaa aaaattagaa aatattgcgt atgccttaat tattggtgga gcgattggga
     2821 atgccattga ccgtgcttac aacggctatg tagttgattt ccttcatttt tattgggata
     2881 tttatcacta ccctgtgttt aatattgccg atattgcgat ttgtgtgggt gcaggcttac
     2941 tgattttaga agcctttata accaaaaaag aaccgaaaaa ataagagtga ttaaatgaaa
     3001 attttattag caaacccacg cggtttttgt gccggcgtgg atcgagccat ttcgattgtt
     3061 gaattagcct tagaaattca cggtgcacct atctatgttc gccacgaagt ggtacataac
     3121 aaatttgtgg tggatggctt gaaagccaaa ggggctattt ttgttgagga gctacacgaa
     3181 gtgccggacg atgctatcgt gatcttctca gcccacgggg tgtcgcaagt agttcgccaa
     3241 gaagccaaaa atcgtggctt aaaagtgttt gatgccactt gcccactcgt gaccaaagtg
     3301 catatgcagg ttgcccgagc cagtaaaaaa gggacaaaag caattttaat cggtcacgaa
     3361 ggtcaccctg aagttatcgg cacaatggga caatacaata accaagaagg tggcatttat
     3421 ttggtagaat cggtggaaga tattgccaac ttgccggttt ccaacaacga tgaattaact
     3481 tttatgacgc aaaccacact ttccattgac gataccattg gcgtgattga ggcgttaaaa
     3541 gagaaatatc cggcaattca aggccctcgc aaaaatgata tttgttatgc taccaccaat
     3601 cgccaacacg cagtgcggga actggcagaa caatcacaat tagtgttagt ggtaggttca
     3661 aaaaactcct cgaactcaaa tcgtttggcg gaactcgcct ctcgaatggg tgtgatgtca
     3721 aaactgattg atggtccgca agatattgat ccaacttggt tggagggtat tgaaactatc
     3781 ggtattaccg ccggtgcttc cgctcctgaa gtgttggtgc aatcagtaat ttcgcattta
     3841 aaaacccttg gcgtaaccga agttgaaacc ttagtcggca acgaagaaga cgtggtgttt
     3901 gaagtcccga aagagttacg catcaaagag gtaaaataat gacagaaatc aaccgcttta
     3961 aattagaatg ggcgtgccga cgtggtatgc gtgagttaga caaaatgatt atgccgtttt
     4021 accaaaatca ttttaatgaa ctcaatgaaa cccaacagca agctttcgtt gaaatgctca
     4081 attacagcga cccggaactg tttcgctggg taatgctgca agcacctgcc ccaaccgaag
     4141 aaatcgctga attgattagg ttaatgcgag ataaaattga gacctagagc taatagatta
     4201 acctcttctt tggggtaaat atatagctac ttaccccaaa gaagactaaa ttcattcaac
     4261 ttttacaaaa taaccctaaa ctagaaaagt taatcttcca atttaataat atgttgctga
     4321 taagctatta aaccttgtat tacatgcact ttctgctgat aggtattgac atagtgatca
     4381 acagcgggtt ttactaataa tgacgctgtt tacctgtctc atctggtgac caaaggtaat
     4441 catcaaaact aataacgcct ccttttctca ctaaacgatg tgcaagtaaa gcatcgaaca
     4501 acacatctgg cgcttcgtga gatccatcta tgtaaatata gtcaaaataa tttatatatc
     4561 catttgcgag aagttcaatc attttttggt gagaatagcc acggtgctta tataacacaa
     4621 cattttggaa tttctgttgc gcaagagtga cattatggat aaatcgttgt tctatttctc
     4681 ccatgtccca aactccttga tgctctgctc ccccttccca actatcaata caatgaattt
     4741 caacttgtga ttgatatttc aatgcttctt caatgaaaaa gacagatgat ctaccttcaa
     4801 atgatccaat ttctaaaatc ctccgaggtt ttacatattc aaaaatagaa gctaatgaag
     4861 gaacattatg agtaaaccaa tcagagttaa aaatatattg gttggctgac tggttggaat
     4921 ggttggaatg g
//
LOCUS       DANLXF010000177         4925 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.177, whole genome shotgun sequence.
ACCESSION   DANLXF010000177 DANLXF010000000
VERSION     DANLXF010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4925
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.177"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(106..600)
                     /locus_tag="PXD66_002000"
     CDS             complement(106..600)
                     /locus_tag="PXD66_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL6320264.1"
                     /translation="MLSGLKIESFSKEFKRDCFDSESELLNQYLKTQVSQDIKRRITS
                     CFLAVNSEQTVVGYYTLASTSLSLSDLPITLQRKLPRYPTVPAVLLGRLAVDKRVKGL
                     GLGQALLGNALKRVLRADIAAYALIVEAKDESAVNFYRKFGFIDFENEARKLFFPLDR
                     VRLS"
     gene            complement(600..872)
                     /locus_tag="PXD66_002001"
     CDS             complement(600..872)
                     /locus_tag="PXD66_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HDL6320265.1"
                     /translation="MATATARFEARMSQEVHMLLKRAASLEGRSLSDFVVGAALSAAR
                     KTVEQNDIIRLSVNDQELFAKALIDPPEPNLAMQKALALSSQILGE"
     gene            complement(1031..1459)
                     /locus_tag="PXD66_002002"
     CDS             complement(1031..1459)
                     /locus_tag="PXD66_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320266.1"
                     /translation="MRKLLVITALTLCTTPVFAADKNVIFSCTSTEGKPLTVKRVGND
                     YEYSYDKTTFKNPIKKAVTNDGSIIARGSGFTTYALELENDGLKYLVGFVQPNGNAKE
                     FIEPGATISQSKEQPSIGSVDCDTRKKSHYKFDVHLMNTL"
     gene            complement(1480..3486)
                     /locus_tag="PXD66_002003"
     CDS             complement(1480..3486)
                     /locus_tag="PXD66_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HDL6320267.1"
                     /translation="MKSQFKFKKTHLLVITVLFSSTSSISFADSITDKLEFSKSDSII
                     SDRVFYQIGGGTGYMAPPSRGNTKVVDLGIGWKANLTCGNFDIKTTIKNDLNGLKDGF
                     KDLMGNVIDSAKGAVASMPAMILQRANPQLYDLLTNGMYQGKLDFNRLKTSCEEMSKV
                     LADHTLGGKWGQAADLESYKQITSTQPDAKQAQKDLEDKKGKDGRVWVGGQRKGGQNQ
                     DPINVVGDVAKGGFNLLNKRNVLDSSPVSNCEGNLCQTWDTPQAAADFLTKIIGEQSI
                     STCKEDCGTKTSSKAGVGLSPEIEEEHIRTIVKLENVLNMDVPKSELLAELSSSSVPV
                     TRGLIESLRDDPDVELLSQRLSAEIATSKVMEKMLLARRAILAGMREPNVAQNADAQV
                     ELERVLNIIDREISQVKLEMDMQKMITGNTASVILQSKSEREALAGAHSLSQDSLDKR
                     VQGLENGNVALTDLETGSNINLSRRNIALSIPNIPNVAALIPTLPNRGGSGVGGNFPS
                     LAQISGSTMDQATGLLRNFEGFISKAQWDVNAHRVGYGSDTITRADGTVVKVTKDTVI
                     TKEDAERDLARRTAIFANNVRQELGDSHWNALPANAQAVLVSYAYNYGSLAKTKSVLD
                     AARRSAQTGDMTALATAVRNRQVDNNGINARRRNQEADYILGKN"
     gene            complement(3501..4442)
                     /locus_tag="PXD66_002004"
     CDS             complement(3501..4442)
                     /locus_tag="PXD66_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HDL6320268.1"
                     /translation="MKRAFFSVLLSVSLAVPTTLYPQQVKASLNTASIAASSASTSCL
                     DYKVVGTCFWLFCTYFGCKVRTSTKIKHYLPEMVVSSYNQDGQTPWTELQSLNPGVKG
                     GEYRSPQRQYSQLTFKNADAFGHPQGAIGQLLGSTGYYCKSQTTPFIPYFLSGLDFFA
                     WRENMPEMFYPEALIPGMREVGRVGDSWGNIYPRSGWVTQAHDYKASATIVQRVADIV
                     TRTAQPHIYFPAAKKGGVGEWPPGPVEEGQARTHKWQMLYPQMSNTCSVFPNGGQTDT
                     YSSQISNQGDYAWALWRPYSCCKRRGQTFLYSIDWSH"
     gene            complement(4439..4882)
                     /locus_tag="PXD66_002005"
     CDS             complement(4439..4882)
                     /locus_tag="PXD66_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03757 family integrating conjugative element
                     protein"
                     /protein_id="HDL6320269.1"
                     /translation="MKASRFLGYFSIVTVVFSVVAQAQPNIIVYTTKMYPIQNSQLAS
                     QVYYLDQVENFEEEISRNLSKSPEIAEREIRAFFNSADWKAQEEQLKDAYQGVISGWQ
                     NGIKKVPAVFFNNLQSSPSVIYGMTDIQKALDEWHKWVQQHGESK"
BASE COUNT         1383 a         1068 c          899 g         1575 t
ORIGIN      
        1 tataatatat gtatatttga gaaaagtggg atgattccat tctttaaaag ctcgaatttt
       61 cctgcctaac caataaccaa aattattttt cataatcact ctccattaag ataatctcac
      121 cctatctagt ggaaaaaata atttcctagc ctcattttca aagtcaataa atccaaattt
      181 acgataaaaa ttgaccgcac tttcatcttt tgcctcaaca attaatgcgt aagctgctat
      241 atctgcacga agtacccttt taagtgcatt acctaataga gcttgcccta accctaatcc
      301 ctttacacgc ttatctactg ctaatcgacc aagtaggacg gcaggtaccg taggatatct
      361 tggtaatttc ctctgtaatg taattggtaa atcagataat gaaagacttg ttgaagccag
      421 tgtgtaatat cctactactg tctgttcaga attaacagct aaaaaacaag aagtaatacg
      481 tcttttaata tcttgtgaaa cctgagtttt gagatattga tttaataatt cagattcact
      541 atcaaaacaa tctcgtttaa attccttgct aaatgactct attttcaatc ccgaaagcat
      601 tattccccca gaatctgtga gcttaatgcc aaggcttttt gcattgccaa attaggttca
      661 ggaggatcaa tcaatgcttt agcaaaaagt tcttggtcat tcacagataa tcgaataata
      721 tcattttgct caactgtttt tcttgctgca gatagtgcag ctccaacaac aaaatcacta
      781 agactacgcc cttcaagaga agcggctcgt tttaacagca tatgaacttc ttggctcatt
      841 ctagcttcaa atcgtgcggt tgcagttgcc atatattttc tccttgaatt atttagtttt
      901 aattgtacgt atttataccg tacaaatcaa gcatgacaat aaaaaaatcc cttagatttt
      961 caaacctaag ggatttacct accgatcttt gtgtttttta acgtgggctg gccctagtgc
     1021 catacaggca ttataaagta ttcatcaaat gaacatcaaa cttataatgg ctttttttac
     1081 gagtatcaca atcaacagag ccgatacttg gttgttcttt actttggcta atcgttgctc
     1141 ccggctcaat aaattctttt gcattgccat ttggctgaac aaagccgacc aaatacttca
     1201 atccatcatt ttccagctct aatgcatacg ttgtaaaacc tgaacctctg gcgataattg
     1261 agccgtcatt agtgactgcc tttttaatcg gatttttgaa ggttgttttg tcatacgaat
     1321 attcgtaatc atttcctacc cgtttaacag taagcggttt tccctccgtg ctggtacagg
     1381 aaaatattac gtttttatct gctgcaaata ctggggtcgt gcagagtgta agagcagtaa
     1441 ttactaataa tttacgcata aatgtaactc cttataaggt taatttttac caagaatata
     1501 atcagcttcc tgattccggc ggcgtgcatt gataccgtta ttatcaacct ggcgatttct
     1561 gacagcagtt gccaaagcag tcatatcacc agtttgagct gatcgtctag ctgcgtcaag
     1621 cacacttttt gttttcgcta gtgagccgta attatatgca taagacacca aaacagcctg
     1681 agcgttcgcc ggtaatgcat tccaatgtga atcacctaac tcttgtctca cattgttcgc
     1741 aaagatagca gttctgcgag ctaaatcacg ttcagcatct tctttggtaa tcacagtatc
     1801 cttggtcact ttaactacgg taccgtctgc ccgtgtaatc gtatcggaac catatcctac
     1861 acgatgggca ttcacatccc attgagcctt ggagataaag ccttcaaaat tacgcagtaa
     1921 ccctgttgcc tgatccatcg ttgaaccgct gatttgtgca agtgatggga aatttccgcc
     1981 tacacctgaa ccacctctat ttggtagagt cgggatgaga gctgctacat tcggaatatt
     2041 tgggatactc aatgcaatat ttctacgaga gaggttaata ttagagcctg tctctaaatc
     2101 agttaatgca acattaccgt tttcaagtcc ttgaacacgc ttatccagac tatcttggga
     2161 taaactatga gcaccagcaa gagcttcccg ttcggattta ctttgcagaa tcaccgaagc
     2221 cgtattgccg gtgatcattt tttgcatatc catttccagt tttacttggg aaatttcacg
     2281 gtcaataata ttgagtactc tttccagttc tacttgagca tccgcatttt gagcaacatt
     2341 cggctctcgc ataccagcta ggattgctcg gcgagccagt agcatttttt ccattacttt
     2401 actggtcgca atttcagcgg ataaacgttg gctgagaagt tcaacatctg gatcatcacg
     2461 gaggctttca atcaagccac gagtgacagg tacactactt gagcttaatt cagctaatag
     2521 ctcggatttc ggcacatcca tattcagtac attttctaat ttaacaattg tacgaatatg
     2581 ttcttcttca atttccggac ttaaaccaac accggctttc gaactggttt tagtaccaca
     2641 atcctcctta caggttgaaa ttgattgctc accaataatc ttagttaaga agtcggcagc
     2701 agcttgaggt gtatcccaag tctgacaaag attaccttca caatttgaaa ccgggctgct
     2761 atctaagaca ttccgcttat tgagtaaatt aaatccgcct ttcgctacat caccaacgac
     2821 attaattgga tcttggtttt gcccaccttt acgttgccca ccaacccaaa ctctcccatc
     2881 tttccctttt ttatcttcta ggtctttttg ggcttgcttc gcatcaggtt gtgtcgaggt
     2941 aatttgctta tagctctcta aatctgctgc ttgcccccat tttcctccta gtgtgtgatc
     3001 tgccagaact ttagacattt cctcacagga agtttttaga cggttgaaat ccagcttacc
     3061 ttggtacata ccatttgtca gcaaatcgta gagttgaggg ttggcccgtt gcaatatcat
     3121 tgcaggcata ctggcaaccg cacctttagc tgaatcaatg acattcccca ttaaatcttt
     3181 aaagccatct ttaaggccgt ttaaatcatt tttaatagtt gttttaatgt caaaattgcc
     3241 acaggttaaa ttcgctttcc aaccgatgcc taaatcaacg actttggtat taccacgact
     3301 aggtggagcc atataacctg ttcctccgcc gatttggtaa aagacacgat ctgaaataat
     3361 cgaatccgac ttactaaatt ccagtttatc agtgattgag tcagcaaaag agatagagga
     3421 agtactggaa aaaagtactg ttattactaa tagatgtgtt ttcttgaatt taaattgtga
     3481 tttcattgca agacctcgtt ttagtgtgac caatcaatac tgtaaaggaa tgtttgccct
     3541 cggcgtttac aacaactata aggtcgccat agtgcccaag cataatcacc ttgatttgaa
     3601 atctgagagg aataggtgtc tgtttgcccc ccatttggaa aaacagagca ggtatttgac
     3661 atttgaggat aaagcatttg ccatttatgt gttctggctt gtccttcttc gacaggtcct
     3721 ggtggccatt ctccaacacc accttttttc gctgccggga aataaatatg tggttgagca
     3781 gttcgggtca cgatgtctgc aactctctgc acaatggttg cagaggcttt ataatcgtga
     3841 gcttgtgtta cccaaccgga acgtgggtaa atatttcccc agctatcgcc tacacggcca
     3901 acttcccgca ttcccggaat cagagcttcc ggataaaaca tttccggcat attttcacgc
     3961 caagcaaaga aatctaatcc acttagaaaa tagggtataa atggtgtggt ttgactttta
     4021 caatagtagc ctgtacttcc taataactga ccaattgctc cttggggatg cccgaaagca
     4081 tctgcatttt tgaaggttag ttgagagtat tgacgttgcg gagaacgata ttctccgcct
     4141 tttacgccag gattgagaga ttgtaattct gtccacggag tttgcccatc ctggttataa
     4201 gatgaaacga ccatttccgg taaataatgc ttaattttgg ttgatgtacg aaccttgcat
     4261 ccaaagtagg tgcaaaataa ccagaagcac gttccgacga ctttatagtc taaacagctt
     4321 gttgaggcac ttgatgcagc aatcgacgcc gtattcaatg atgcttttac ctgttgagga
     4381 tataaggttg ttggtactgc taaacttaca gagagaagta ccgaaaaaaa cgctcttttc
     4441 attttgattc accgtgttgt tgaacccatt tatgccattc atctagtgct ttttgtatat
     4501 cagtcatacc ataaatcacg ctcggagagg attgcagatt attaaaaaac accgcaggca
     4561 cttttttaat tccattttgc catccactga tcaccccttg ataagcatct tttaattgct
     4621 cttcttgggc tttccaatct gctgaattaa agaatgctcg tatttctcgt tcggcaatct
     4681 cgggggattt actgagattt cgactgattt cctcttcaaa attctctact tgatcaagat
     4741 aataaacttg ggaggcaagt tgtgaatttt gaatggggta catctttgtt gtatagacaa
     4801 tgatattcgg ctgtgcttgt gccactacag aaaacactac tgttactatg ctgaaatatc
     4861 ctagaaatct acttgctttc atcactctct ttccgaataa ataagcagaa aattagcgag
     4921 atttt
//
LOCUS       DANLXF010000178         4883 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.178, whole genome shotgun sequence.
ACCESSION   DANLXF010000178 DANLXF010000000
VERSION     DANLXF010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.178"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..2181)
                     /gene="yccS"
                     /locus_tag="PXD66_002006"
     CDS             complement(<1..2181)
                     /gene="yccS"
                     /locus_tag="PXD66_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HDL6320270.1"
                     /translation="MNKFLRYLQHSWLSENVIKTLPMFISLNIAAVSIWQLGISDFAM
                     PLMLGIIAGGLVDLDNSLIGRAKNLIISLVAFSLSSIGASVSLHLGWLFVPVAVVCTF
                     LLVMLGAIGQRYSTIAFGTLIVAVYNSLAYTPEVLWYHNVVMILLGTLLYGLVGILVY
                     LIFPNRTVQENLASAYLALSHYLLAKSAYFDPDDDDLAAKQLALAKANSEVMAAFDKT
                     RVALFYRLSRQHKQQRTQRMLRYYFTAQDILERASSSHYQYHELFQQLSNSDLMFRFQ
                     RVLELQAAACQKIAMSLRHREVYEHGVRGEKALQGLLNSLNYHQERGLKSAYRWRAIA
                     ENLGNIERQLAQIEQGSSSGEHIEPLSKNFTKSHRLIAENITGLSNISQAIKAHLTFE
                     SQLFRHAVRLSIVVLLCSAIVPILGFDSKGYWILLTAIFVCQPNYTATKTRLIQRVIG
                     TIFGVLVGGSFELFNVALSLEAQLGLIVLTGSLYTFFRLVHYGFSTFYITVLVMISLD
                     IAGVSLESGILMRMLDTLLGTAIAWLAVAFLWPDWKYLNLQRNLHNGLKASGQYLRHI
                     IAQLQFGYNEQLPYRIARRDVHNHLSALSNAITNMYSEPQKYANDLTFAPKLLGIAYT
                     LLGYISTLGAYRIASREINQQVDFSAMFFSHAREVANIIGQIASESRSFAKLSEKLTA
                     IDQDLTQFEQENQQKQNDLALVLIQQLRLIVQLLPQLNSILQLKS"
     gene            complement(2225..2683)
                     /locus_tag="PXD66_002007"
     CDS             complement(2225..2683)
                     /locus_tag="PXD66_002007"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDL6320271.1"
                     /translation="MKTTVRTIAKQKQIALVAHDSCKQDLIRWTQTHQAQLASHKLYA
                     TGTTGHLLARETGLEITSLLSGPMGGDQQLGGLIAEKKIDLLVFFWDPMNAAPHDPDV
                     KALMRIATVWNIPVAINETTANFLLNADLFNQAISVNVPDYDGYLAERLG"
     gene            2809..4083
                     /gene="thrC"
                     /locus_tag="PXD66_002008"
     CDS             2809..4083
                     /gene="thrC"
                     /locus_tag="PXD66_002008"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDL6320272.1"
                     /translation="MNLYNIKHPEEQVNFAQAVRQGLGKNQGLFFPEVLPKLDSIDEL
                     LDLPLVKRSQKILSALIGEELPQETLNAMVKNAFTFPAPLAKVNDDIYALELFHGPTL
                     AFKDFGGRFMAQALANVRGDGKITILTATSGDTGAAVAHAFYGLENINVVILYPKGKI
                     SPLQEKLFCTLGGNIRTVAIESDFDACQALVKQAFDDAELRQAIGLNSANSINISRLL
                     AQICYYFEAVAQLPKEKRDNVVVSVPSGNFGNLTAGLIAKTLGLPIKRFIASTNANDT
                     VPRYLQSGKWEPNATVATLSNAMDVSRPNNWPRVEELFKRNGWSLSELGSAALNDAET
                     EAALKAQYAEGYLCEPHGAIAYQSLKDQLQQGETGIFLCTAHPAKFKESVEHILAIEL
                     PLPEALDKHNKLPLLSDEMAADFATLRAYLLK"
     gene            complement(4162..4881)
                     /locus_tag="PXD66_002009"
     CDS             complement(4162..4881)
                     /locus_tag="PXD66_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDL6320273.1"
                     /translation="MTGQKVPSVTFRTRQNDAWVDVTTDELFNNKTVVVFSLPGAFTP
                     TCSSTHLPRYNELACEFKALGVDDIICMSVNDTFVMNAWKADQESENITVIPDGNGEF
                     TEGMGLLVDKNDLGFGKRSWRYSMLVKNGIVEKMFIEPQEPGDPFKVSDADTMIKYLS
                     PNWTAKESVSIITKPGCLFCAKAKALLKEQGYTFEEIVLGRDASTISVRAITGKTSVP
                     QVFIGGKYIGGSDELAAYFAK"
BASE COUNT         1431 a         1119 c          992 g         1341 t
ORIGIN      
        1 actcttgagc tgtaatatac tatttaactg cggtaataac tgcacgatca gtctgagttg
       61 ttgtatcaac actaaggcga ggtcattttg cttttgctga ttttcttgct caaattgggt
      121 gaggtcttgg tcgatagcgg tcaatttttc cgataatttt gcaaatgaac ggctctctga
      181 ggcaatttgc ccgatgatgt ttgccacctc tctggcgtgt gagaagaaca ttgcggaaaa
      241 atccacttgt tgattaatct ctcggctagc aatacggtaa gcccctaagg tggaaatata
      301 acccagcaag gtgtaagcaa tacctaacaa ttttggggcg aaggttaaat cgttcgcata
      361 tttttgtggt tcgctgtaca tattggtaat cgcattactc agtgccgata aatggttatg
      421 cacatcacgg cgggcaatac ggtaaggcag ttgctcgtta taaccaaact gcaactgggc
      481 gataatatgg cgtaaatatt gcccactggc tttcaagccg ttatgtaagt tacgttgtaa
      541 atttaaatac ttccaatccg gccataagaa tgccactgcc agccacgcaa tcgctgtacc
      601 tagtaaggta tcaagcattc gcattaagat gccgctttca aggctcacgc ctgcaatatc
      661 aaggcttatc attaccaaca cagtgatata aaaggttgaa aagccataat gtaccaagcg
      721 gaaaaaggta taaagcgaac cggtcagtac gattagccca agctgggctt cgaggctgag
      781 cgccacgtta aacagttcaa aagagccacc aaccagcacg ccgaaaattg tgccgatcac
      841 acgctgaatc agtcgggtct tagtagcagt gtaattcggt tggcagacaa aaatggctgt
      901 gagtaaaatc cagtagcctt tgctatcaaa gcctaaaatc ggcacaatcg cactgcacaa
      961 cagcaccact atcgacaacc gaacggcgtg tcgaaacagt tgggattcaa aggttaaatg
     1021 ggctttaatt gcttggctga tattgcttaa gccggtaata ttttcggcga tcaagcggtg
     1081 agattttgtg aaatttttac ttaacggttc aatatgctca ccactgcttg agccttgttc
     1141 aatctgggct agttgccgct caatattgcc taaattttct gcaatagcac gccagcgata
     1201 agcacttttc aagccccgtt cttgatgata attcaatgaa ttaagcaagc cctgcaaggc
     1261 tttttcgcca cgaacgccgt gttcatatac ttctctatgg cgtaacgaca ttgcaatttt
     1321 ttgacacgcc gcagcctgta attccagcac ccgttgaaag cggaacatta aatcactgtt
     1381 acttaattgt tggaacagtt cgtgatattg ataatggctg gaacttgccc gctctaaaat
     1441 atcttgagcg gtgaaatagt aacgcaacat tcgctgtgtc cgttgctgct tgtgctggcg
     1501 gcttaggcgg tagaataggg caactctggt tttatcaaag gcagccatca cctcactatt
     1561 ggctttcgcc aaggctagtt gtttggcagc taaatcgtca tcatcagggt caaaataggc
     1621 agatttcgcc agtaaataat ggctcaacgc aaggtaggcg gacgctaaat tctcctgcac
     1681 ggttcgattc gggaaaatca ggtacaccaa aataccgacc aaaccataga gtaaggtgcc
     1741 aagtaagatc atcaccacat tgtgatacca caatacttcg ggcgtataag ccaggctgtt
     1801 ataaaccgcc acaatcagcg tgccaaaagc aatggtacta tagcgttgcc cgattgcccc
     1861 taacattacc agcaaaaagg tgcaaaccac cgccaccggc acaaacagcc agcccaaatg
     1921 gagcgacaca ctcgccccaa tcgaggaaag cgagaacgcc actaggctga taatcagatt
     1981 tttcgctcgt ccgattaggc tgttgtctaa atccaccaaa ccgccggcga taatgcctaa
     2041 catcaacggc atcgcaaaat cagaaatacc gagctgccaa atgctgaccg ccgcaatgtt
     2101 tagcgaaata aacatcggca gggttttaat tacattttcc gaaagccaac tgtgttgtag
     2161 atatcttaaa aatttattca tatttgctca tttcgggcag gcacaggacc tgcccctacc
     2221 tacactaccc caaacgttct gccaaatagc catcataatc cggcacattc acgctgattg
     2281 cttgattgaa caaatccgca tttaacagaa aattagcggt ggtttcgtta atcgccaccg
     2341 gaatattcca aacggtcgca atccgcatca gggctttcac atcaggatcg tgcggagcgg
     2401 cattcatcgg atcccaaaag aacaccagca gatcaatttt cttttctgcg atcagcccgc
     2461 ctaattgctg atcgcccccc atcgggccgc ttaacaatga agtaatttca agccctgttt
     2521 ctctggcgag caaatgcccg gtagtgccgg tggcatatag cttatgcgag gcaagttggg
     2581 cttgatgagt ttgtgtccaa cgaattaaat cttgtttaca gctatcgtga gccactaagg
     2641 caatctgttt ttgttttgcg atggttctga cggtggtttt catttttagc tcctttgcaa
     2701 aatttttcac aaatcatacc gcttattttt cgcttttgca ttgactaatc acggcttatg
     2761 gctaaaatca ggtaaaaatt cgcattttaa aacaaaggat taaccgcaat gaacttatac
     2821 aacatcaaac accctgaaga acaagtcaat ttcgcccaag cggtacgcca aggcttaggc
     2881 aaaaatcaag ggctattttt ccctgaagtg ttacctaaat tagatagcat tgatgaatta
     2941 ttggatttac ctttggttaa acgcagccaa aaaattcttt cagccttaat cggcgaagag
     3001 ttaccgcaag aaaccttaaa tgcaatggta aaaaatgcgt tcactttccc ggcaccgctt
     3061 gccaaagtca atgatgatat ttatgcgtta gaattatttc acggtccgac tttagccttc
     3121 aaagattttg gtgggcgctt tatggctcaa gccttagcca atgtacgagg agacggcaaa
     3181 attactattt taaccgctac ctcaggtgac accggtgcgg cggtggctca tgctttctat
     3241 ggtttggaaa atatcaacgt ggtcattctt tacccgaaag gcaaaatcag cccattacaa
     3301 gaaaaattat tctgtacctt aggcggtaat attcgcactg ttgcgattga atccgacttt
     3361 gatgcttgcc aagccttagt gaaacaagcc tttgatgatg ccgagctacg ccaagcgatt
     3421 ggtttaaatt ccgcaaattc catcaacatt agccgtttac tggctcaaat ttgctactac
     3481 tttgaagcag ttgctcagtt accaaaagaa aaacgagata acgtagtggt ttcagtacca
     3541 agcggtaatt tcggtaactt aaccgccggc ttaattgcca aaacattagg tttaccgatt
     3601 aaacgtttta ttgcctcaac caatgccaat gacaccgtgc cacgctattt acaatcaggc
     3661 aaatgggaac caaacgcaac cgttgcaacc ctttctaatg caatggacgt aagccgcccg
     3721 aacaactggc cacgagttga agaattattt aaacgcaatg gctggagttt gtctgaattg
     3781 ggttctgccg cattaaacga tgccgaaacc gaagcggctt tgaaagctca atatgcagaa
     3841 ggttatctgt gtgagcctca cggtgctatt gcttaccaaa gcttgaaaga tcagctacaa
     3901 caaggcgaaa ccggtatttt cttatgcacc gctcacccgg caaaattcaa agagtcggtg
     3961 gaacacattt tagcgattga attgccgtta ccggaagcat tagataagca caacaaactg
     4021 ccactgttat ccgatgaaat ggcagcagat tttgcaacat tgcgtgcata tttgttgaaa
     4081 taaatattca ataatagaaa agggattaag tattaaactt aatccctctt tttatgcatt
     4141 acaagcggtt atatttgcct attattttgc aaaataagca gctaactcat cactaccgcc
     4201 aatatattta ccgccgataa atacttgtgg cacagaggtt ttacctgtaa ttgctcgtac
     4261 agaaattgtg cttgcatcac gacctaacac aatctcctca aaagtatagc cttgctcttt
     4321 taataatgct tttgcttttg cacagaacag gcaacccggt ttagtgataa tcgaaacaga
     4381 ctctttagct gtccaatttg gacttaaata tttaatcatt gtatcggcat ctgatacttt
     4441 aaacggatcg cccggttctt gtggctcaat aaacattttt tccacaatac cgtttttcac
     4501 cagcatcgaa taacgccaag aacgtttacc aaacccaagg tcatttttat caactaatag
     4561 ccccatacct tctgtgaact caccattacc atcaggaata acagtaatat tttcagattc
     4621 ttgatctgct ttccaagcgt tcattacaaa ggtatcatta actgacatac agatgatatc
     4681 atctacccct aatgccttaa attcacaagc taattcatta tagcgaggca aatgtgttga
     4741 tgaacaagtc ggtgtaaatg ctccgggtaa tgaaaacaca accaccgttt tgttattaaa
     4801 taactcatcg gttgtgacat caacccaagc atcgttttga cgtgtacgaa atgttacact
     4861 gggtactttc tgacccgtca tat
//
LOCUS       DANLXF010000179         4868 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.179, whole genome shotgun sequence.
ACCESSION   DANLXF010000179 DANLXF010000000
VERSION     DANLXF010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4868
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.179"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(295..942)
                     /gene="rnt"
                     /locus_tag="PXD66_002010"
     CDS             complement(295..942)
                     /gene="rnt"
                     /locus_tag="PXD66_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010130043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HDL6320274.1"
                     /translation="MEQTTEQIDYKLLKHRFRGYLPVVIDVETAGLNAQTDALLELAA
                     ITLKMDEQGYLVPDQQCHFHIAPFEGANINPESLKFNGIDIDNPLRGAVAENIAIPEM
                     FKMVRKAMKEQGCQRAVIVAHNAAFDQGFLQAAIKRINAKRDPFHPFAMFDTATLAGF
                     MYGQTVLVKACQVAKIPFDGKQAHSALYDTERTAELFCAMVNRLKDLGGFPPLSD"
     gene            complement(1027..1434)
                     /gene="gloA"
                     /locus_tag="PXD66_002011"
     CDS             complement(1027..1434)
                     /gene="gloA"
                     /locus_tag="PXD66_002011"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HDL6320275.1"
                     /translation="MRILHTMLRVGDLERSIKFYTDVLGMRLLRRSENEQYKYSLAFL
                     GYADESESAVIELTYNWGVDKYELGTAYGHIALGVDDIYKTIEDVRAAGGKITREPGP
                     VLGGTTVIAFAEDPDGYKIEFIENKDAKAALGN"
     gene            1524..2339
                     /locus_tag="PXD66_002012"
     CDS             1524..2339
                     /locus_tag="PXD66_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320276.1"
                     /translation="MAHDHDSPSKLEISTHAALPMVLMLFIEMALNFYSAPKNSIFIS
                     PYLLAFFVTSLISFLVLWKGEICRGQASRLSFILPFLLIFAVGNFLYSIGFTPKHNPM
                     AVASIAAVFACLIYFRLPEDQTLYNTLMYCGLAISSIGIIQYLAIYWFELPSLFNGIR
                     ANNFAQVLLGILLAGWYLMLAKSRLDVFLKLLVKTALVVLILNYCWTAFVLYQHLQIM
                     PDMPLLSFIIYFAAQFVILLMLAWLLLGKNIKNPTAWTVATLLAILYPFANVV"
     gene            2343..3089
                     /locus_tag="PXD66_002013"
     CDS             2343..3089
                     /locus_tag="PXD66_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320277.1"
                     /translation="MKRALKFFGIATVLSVVAIYYFLMPSHYLATPINWILPKAWKVQ
                     LPQGLNLNWQGSSLAQFQLSYRDCPLLSADKTDLGWIEKNHLSVDKLDIDYRCLALFP
                     QNEHSNSDTSLNALLAVIPDGEATIHALNWKNLPTDFNPRLQRLFEQTAIKLSLSKGE
                     IQATAAQQAVKFAANFANGRLNADLSYQPNEKEQHNLRISAEIEDNFTQLPPTISAEY
                     DWQLSYEIIANKALQKGALFSVGKKMPHKS"
     gene            3275..>4868
                     /locus_tag="PXD66_002014"
     CDS             3275..>4868
                     /locus_tag="PXD66_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH domain-containing protein"
                     /protein_id="HDL6320278.1"
                     /translation="MPLKTAIRISLLSQNSWGKGNIVISNSDGEITEKGLNLPLRLTG
                     NIKHGNFILYSSVPLDFQGNFDDLSVKFLPSSLLRLTGKERYLTIEDLRFPLAGIKID
                     KHGIHGRLHAILRGQSPDFNNIEFHLDGQAQNFKAGALDFFQDPKDANAVKDSWKWRI
                     WGASILKAFNSKVNLSGRGQWHKQLVRLEELKGDLATVKLADTTISKITLNNTQAIRF
                     NVKKFTLDGAVMLQSPEIAFSYGGVLPKPRVNLAFDGEVEKLRFKGAVNTTELGPLKL
                     FARRQLSQDASQIIGKLYWPEQSAQGFQPLFPFRSQWVINNGTIRGETAFSAGSKNGL
                     IAGGHLAIRNGGLSLPNGEAKGIEFSLPYRYQNGRFHFGAKKPLDVKIAQIKLGDLAL
                     DNASMKLNGYYPYTKAKPLNLNQLSFDLFGGKLNVKRFALPQTELAYLNLERIDFEQI
                     LQFMQYQQIDLKGKANAILPFWLSGKPCYICDGLLTQAETSYLTFTPELLSEMQKSGY
                     TEQILLHLVDKSTINDFRSLINV"
BASE COUNT         1418 a         1080 c          931 g         1439 t
ORIGIN      
        1 attagataaa aaataaggag ggtcgaaata gcgggtatac agaaagtgct tatagccaaa
       61 aaatagtcta ttgttattga atacaagcgg tctattttgt cacatttttg ccaaatttgg
      121 ttaaaaaacc aaccgcttgt aatcttaact gaagatagat agttttttat tttttctaca
      181 actctacagg gctggattta ttgcagaatg gtacttgcca ctaactatca tcactcccag
      241 gataatttat agaaacaata cgagaaattg tgttaataaa tgaaatgaat aacgttaatc
      301 cgataatggt gggaaaccac ctaaatcttt taaacgattg accattgcac agaaaagttc
      361 tgccgtgcgc tcggtatcat acaacgcaga atgcgcttgc ttaccgtcaa acgggatttt
      421 tgccacctgg caggctttta ccaacacggt ttgcccatac ataaaaccgg caagggtggc
      481 ggtgtcaaac atcgcgaaag ggtgaaaggg atctcgcttg gcattaattc gtttaatcgc
      541 agcctgtaaa aagccctgat cgaacgctgc attgtgggca acaatcaccg cccgttggca
      601 gccttgctcc ttcattgctt tgcgaaccat tttgaacatt tccggaatcg caatattctc
      661 cgcaaccgct cctcttagag ggttgtcaat atcaatgccg ttgaatttta atgactcagg
      721 gttaatattc gccccttcaa atggcgcaat atgaaaatgg cattgttgat ccggcactag
      781 ataaccttgc tcgtccattt ttaaagtaat tgccgccaat tccaataagg catcagtttg
      841 ggcatttaag cctgctgttt ccacatcaat cacaaccggt aaatagccac gaaaacgatg
      901 ctttaataat ttataatcta tttgttccgt tgtctgttcc atttatctat caacctaaaa
      961 tacaagcggt ctgatttgca aaatttttta caaatccgac cgcttgaata ttcatattcg
     1021 caaaaattag tttcctaacg ctgctttcgc atctttattc tcaataaatt cgattttata
     1081 accgtccgga tcttcagcaa aggcaatcac agttgttcca cccaataccg ggcccggctc
     1141 acgggtaatt ttaccgcctg ccgcacgcac atcttcaatg gttttgtaaa tatcatctac
     1201 gcctaacgca atatggccat aagccgtgcc tagctcatat ttatccacac cccagttata
     1261 agtcagctca atcaccgcac tttcgctttc atcggcatag cctaaaaatg ctaacgaata
     1321 cttatactgc tcgttctcgc ttctacgtaa cagacgcata cctaacacat cggtataaaa
     1381 cttaattgag cgctctaaat ctccaactcg taacattgta tgtaaaattc gcatattttc
     1441 ttccccctga agttaaaaaa cgacccctat tatggcataa tcttggctta aaatcatttt
     1501 ctttatacct tttgggatta tctatggctc acgatcacga ttcgccgtca aaacttgaaa
     1561 tttcaaccca cgctgcactg ccaatggtat taatgctgtt tattgaaatg gctcttaact
     1621 tctactctgc accgaaaaat tcaattttta tttctcctta cttattggcg tttttcgtga
     1681 cctcattaat cagtttttta gtgctatgga aaggcgaaat ctgccgaggg caagccagcc
     1741 gtttaagctt tattttaccc tttttactta ttttcgccgt agggaatttc ctttattcca
     1801 taggctttac gccaaagcat aacccaatgg cggtggcaag cattgctgca gtttttgcct
     1861 gcttaatcta cttccgcctg ccggaagatc aaaccttata taacacattg atgtattgtg
     1921 gtttggctat atcaagtatt ggtattatcc aatatttagc catttattgg tttgaactgc
     1981 cttcattgtt taacgggatt cgagccaaca atttcgccca agtgctgtta ggcattttat
     2041 tagccggctg gtatttaatg ttagcgaaaa gccgtttaga tgttttcctc aaactgttgg
     2101 tgaaaaccgc ccttgtggtg ctgattctca attattgctg gacagctttt gtgctttatc
     2161 aacatctaca aattatgccg gatatgccat tgctttcatt tatcatctat tttgcagcac
     2221 aatttgtgat tttattgatg ttggcgtggt tattacttgg aaaaaacatc aaaaatccga
     2281 ccgcttggac agttgctacg ctgttagcga ttttataccc tttcgctaat gtagtctgat
     2341 ttatgaaacg ggcattaaaa tttttcggca ttgcaaccgt tctatctgtt gtggcaatct
     2401 actatttttt aatgcccagc cactatcttg ccacgccgat taactggatt ttgccgaaag
     2461 cgtggaaagt tcaactccca caaggcttaa acctgaattg gcaaggctca tcacttgctc
     2521 aatttcaact ctcttatcga gattgcccgc tactaagtgc cgataaaacc gatttgggtt
     2581 ggattgagaa aaaccatctt tctgtcgata aattggatat tgattaccgc tgccttgcct
     2641 tgtttccaca aaatgaacac agcaattccg acacttcact caacgcattg ctggcggtca
     2701 ttcctgatgg cgaagcgact atccacgccc ttaactggaa aaatttgcct accgatttca
     2761 acccaagact tcagcggctt tttgagcaaa cagctattaa actctcactt tcaaaaggtg
     2821 aaattcaggc aactgccgct caacaagcgg tcaaatttgc agcaaatttt gcaaatgggc
     2881 ggttgaatgc cgatttatca tatcaaccga atgaaaaaga acagcataat cttaggattt
     2941 cagcagaaat tgaggacaat tttacccaac tgccgcccac tatttccgct gaatatgatt
     3001 ggcaattatc ttatgaaatc atcgccaata aagccttgca aaagggcgct ctattctcag
     3061 ttggcaaaaa aatgccgcac aaaagttaga aggcaactgg caggttgaac ttgccgaaag
     3121 tgaaaataac aaactggatt tcccttttct gtttgatggg gaaagcctag aaattcaaca
     3181 agggcggttt gattggcact ttacccaaca atttccactg caagggtttg ttagcaccaa
     3241 gctcacaccg aaatcattca accaaaacgg ctttttgcca ctcaaaacgg cgattcgtat
     3301 cagcctgctt tcccaaaaca gttggggtaa aggcaatatt gtaatctcca attcagacgg
     3361 agaaataacc gaaaaagggc tgaatttacc gcttcgtttg acgggtaata tcaaacacgg
     3421 caattttatt ctttacagta gcgtgccact ggattttcaa ggtaattttg acgatttaag
     3481 cgtaaaattt ttgcctagct ccctacttcg tttgaccgga aaagagcggt atttaaccat
     3541 tgaagattta cgttttccac ttgccggaat taaaattgac aagcacggta ttcacggacg
     3601 actgcacgca attttaagag ggcaaagccc tgattttaac aatatcgagt tccatttaga
     3661 cggacaagca caaaatttta aggctggagc cttagatttt ttccaagatc ctaaagatgc
     3721 taacgcagta aaagacagtt ggaaatggcg aatttgggga gcatctatcc tcaaagcctt
     3781 caacagtaaa gtgaatttaa gcggcagagg gcaatggcat aaacagcttg tgcggttgga
     3841 agaactcaaa ggcgatttag ccaccgtaaa gttagcagat acaaccattt ctaaaatcac
     3901 gcttaacaat acgcaagcta ttcgatttaa tgtcaaaaaa ttcacccttg acggagcggt
     3961 aatgttgcaa tcacctgaga ttgccttcag ttatggcggt gtgctaccca aaccacgagt
     4021 gaatctcgcc tttgatggtg aagtcgaaaa actacgtttt aaaggagcgg tgaataccac
     4081 cgaactcggc ccactcaaac tgttcgcccg ccgccaatta agccaagatg ccagccaaat
     4141 tatcggcaaa ctttattggc cggagcaatc tgctcaaggc ttccagccgc tttttccgtt
     4201 tcgtagccaa tgggtgatca acaacggtac aattcggggc gaaacggcgt ttagtgccgg
     4261 cagtaaaaac gggctgattg ccggtgggca tttagccatt cgtaacggcg gtttatcttt
     4321 acctaatggc gaagcaaaag ggattgaatt ttcgcttccc taccgctacc aaaacggacg
     4381 ctttcatttc ggagccaaaa aaccgcttga tgtaaaaatt gcccaaatca aactggggga
     4441 tttagcatta gataacgctt caatgaaact caacggttac tacccttata ccaaagctaa
     4501 gccattgaat ttaaatcaat tatcctttga tttgtttggc ggtaaactga atgtaaaacg
     4561 ctttgcctta ccacaaaccg aactggctta cctgaattta gagcggattg actttgagca
     4621 aattttgcaa tttatgcaat atcagcaaat tgatctcaaa ggcaaagcca acgctatctt
     4681 gccgttttgg ttaagcggaa aaccttgcta tatttgcgat ggcttactga cacaggcgga
     4741 aacttcctat ttaaccttta cccctgaatt gttgtcagaa atgcagaaaa gtggttacac
     4801 cgagcaaatt ttgttacatt tagtagataa aagcacgatt aacgattttc gcagtctgat
     4861 taatgtcg
//
LOCUS       DANLXF010000180         4856 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.180, whole genome shotgun sequence.
ACCESSION   DANLXF010000180 DANLXF010000000
VERSION     DANLXF010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4856
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.180"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            6..1088
                     /gene="serC"
                     /locus_tag="PXD66_002015"
     CDS             6..1088
                     /gene="serC"
                     /locus_tag="PXD66_002015"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HDL6320279.1"
                     /translation="MTEIYNFSAGPAMMPKKVLEKAQQELLNWLDQGTSVMEVSHRGK
                     LFMELAAQSEADLRKLYNVPDNYRILFLQGGARGQFAAIPMNLIGKKGKALYLNSGHW
                     SATAAKEARNFAEIDEINILETEPQLKVSRLDFSDIAEQYDYIHYCTNETISGVEIFD
                     IPNVGNTPLVADMSSNILARGIDINKFGVIYAGAQKNLGPAGITIVIIRDDLIGNARK
                     DTPSIWNYATQRDADSMINTPPTFAWYLCSLVFQHLLAEGGLKATEERNLAKATLLYE
                     YLDSSTFYYNTVAHENRSLMNVTFTTGNDELNAKFVAEATACGLQALKGHKVLGGMRA
                     SIYNAMPIDGVKALIEFMKVFEQRNA"
     gene            complement(1145..2122)
                     /locus_tag="PXD66_002016"
     CDS             complement(1145..2122)
                     /locus_tag="PXD66_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HDL6320280.1"
                     /translation="MTLAVAAIIGGLIILVWSADRFVEGAASTARHFGMTPLLIGIVV
                     IGFGTSAPEMLVSASSALSGSPGIALGNAYGSNITNIALILGLTAIIKPLSVNSDVLK
                     KELPILLAVTLLSAYLVYDANVTQLDAIILLAVFFVYMAWTIITAMKSKNDALSLDVD
                     AELTNSAKMGLGKSILWLIIGLVSLVISSQFLVWGAVEVAKFFGVSDLVIGLTIVAVG
                     TSLPELASSIAAARKGEVDLALGNIIGSNLFNTLAVVGIAGAIEPMQVSAEVFSRDVL
                     MMSALTFLLFIFGINLYRRTEGGRINRLEGLLLFAAYVGYNFYLFKTAG"
     gene            2234..4801
                     /gene="glnD"
                     /locus_tag="PXD66_002017"
     CDS             2234..4801
                     /gene="glnD"
                     /locus_tag="PXD66_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HDL6320281.1"
                     /translation="MMKNSNELKTLLSAFTEQQRQAFQSENVLSLLAQRSQFYDQLLQ
                     ALWQDFGLAQYEHITLLAVGGYGREEMFPLSDLDILVLTEKPLEEAIQQRLNELFALL
                     WDSKLQLGTSVRTLEECIQIGKAEISVATNMLEGRFLLGNKSLWLRLKEALYQPDFWD
                     IKAFFRAKMAEKNERYARYHNTSYNLEPDLKHSPGGLRDLHLISWIMLRHYGVSSFVD
                     LLNKGLLYPEEYKELIAAQAVLFRMRFALHLQLKRYDNRLRFDRQLQLSEILGYQGEA
                     NQAVELMMRDYFQATKSISQLSQLLLNSFEQAYVNELQKNAQKQPLDQYFYRQEQQLF
                     VVERKIFKTQPTTMLDLFFHLTNDPTLIANADTLRQLRLCLQQQHEPMCFLPEMREKF
                     VQLFAQPKVISRAIVPMHQLGFLSAYLPQWKGIEGLMQFDLFHIYTVDEHTVRVMLNL
                     ENFSEDPSRAFFPLCHQLFATLSEKPLLYLAALFHDIGKGREGEHAQVGALDMREFAE
                     LHGFSQAQINDMVWLVAEHLTMSITAQRRDIHDPQIISEFAKKVQNQTALSSLLCLTV
                     ADICATSESLWNDWKASLFTQLYQFTLQQLAQNLNYQAVAQEHRLQALELMKFVLSAD
                     ERIALNAFWQPCPERYFLHHTPTQLVWHALHYVKTRRLPMVLISNEYARGGTEIFVCC
                     QDQPQLFARIAQLLSQKRISIHDAQIITTENGVVLDSFIVSERDGDDDLTDERCMQIQ
                     QAMMKMLSLTETGIKFTKKPVKHLSFKRKTRLRFLPPSSKQQTEFELFTLDREGLLAQ
                     IGYIFNRLRLNLINAKITTIGERVEDFFVVSNDQGKALSEQEQLELKAILITELDSE"
BASE COUNT         1568 a          957 c         1006 g         1325 t
ORIGIN      
        1 gaaaaatgac agagatatat aacttcagtg ccggcccggc aatgatgccg aaaaaagtac
       61 tcgaaaaagc gcaacaagaa ttattaaatt ggctagacca aggcacatca gtgatggaag
      121 tcagccaccg tggaaagcta tttatggagc tggcggcaca atctgaagca gatttacgca
      181 agctgtataa tgtgccggac aattatcgta ttctatttct tcaaggtggt gcaagggggc
      241 aatttgcagc cattccaatg aatttaatcg gtaaaaaagg caaagcgctt taccttaatt
      301 cagggcattg gtcggcaaca gcagcaaaag aagcacgtaa ctttgcggaa attgatgaaa
      361 tcaatatttt agagactgaa ccgcaactta aagtgagtcg tttagatttc agcgatattg
      421 cagagcaata tgattatatc cattattgca ccaatgaaac catcagtggg gtagaaattt
      481 tcgatattcc gaatgtcgga aataccccgt tagtggcaga tatgtcgtct aatatcttag
      541 cccgagggat tgatatcaac aaatttggag taatttatgc cggagcgcaa aaaaatctag
      601 ggcctgctgg cattacgatt gtgattattc gtgatgattt aattggcaat gcacgcaaag
      661 ataccccatc tatttggaat tatgcaactc agcgagatgc tgattcgatg attaataccc
      721 cgccaacttt tgcttggtat ttatgttctt tagtgttcca acatttactg gcagagggtg
      781 gtttgaaagc tactgaagaa cgtaatcttg ctaaagctac attgctttat gagtatttgg
      841 atagttcaac attctattac aatacggtag cacacgaaaa ccgttcctta atgaatgtaa
      901 cctttaccac gggtaatgac gagctaaatg caaaatttgt ggcagaagcg accgcttgtg
      961 gtttacaggc attgaaaggc cataaagtat taggtggaat gcgtgcctca atttataacg
     1021 caatgccgat tgatggggta aaagcactca ttgaatttat gaaggtattt gaacaacgta
     1081 atgcttaatt caagcttaag aatataaatt aaagagccac gtttgtggct ctttgaattt
     1141 ggcactagcc cgcagtttta aacaaataga aattatagcc tacataagcg gcaaagagta
     1201 ataagccctc taaccgatta attctgccac cttctgtccg acgatagagg ttgatcccga
     1261 aaatgaataa taagaaggtg agagccgaca tcatcaagac atcgcgagaa aacacttccg
     1321 ctgaaacttg cattggttct atagcaccgg caatccccac cacggctaac gtattaaata
     1381 agttagaacc gataatgtta cctaaagcta aatccacttc tcctttacga gcagctgcaa
     1441 tagaagaggc taattcaggt agtgaggtgc ctaccgccac aatagtaagc ccgattacca
     1501 aatcactcac accaaaaaat ttagccactt caactgctcc ccagactaag aattgggaac
     1561 taattactag cgatactaaa ccaataatta gccaaagaat ggattttcct aatcccatct
     1621 tagcactatt ggttaattct gcatcaacat ctaagcttaa tgcatcattt ttacttttca
     1681 ttgcggtaat aattgtccac gccatatata caaaaaacac agcaagtaaa ataatcgcat
     1741 caagttgagt gacgtttgca tcataaacca aataagcaga aagcagggtg acagccagta
     1801 aaatcggcaa ctcttttttt aacacatcgg aattaactga tagtggttta attattgcgg
     1861 ttaaacctaa aatcagcgca atattggtaa tgttagaacc gtaggcatta ccgagagcga
     1921 tacccggcga tccgcttaat gcagaagatg cagatacgag catctccggt gcagatgtac
     1981 caaaaccaat gaccacaata ccgattaaca atggtgtcat accaaaatgg cgagccgtag
     2041 aggctgcccc ttccacaaaa cgatccgcac tccaaactaa tataattaag ccaccgataa
     2101 tagcggcaac agcaagtgtc ataaactatc cttttagatg aaaaacataa aggcattata
     2161 agcattttag ccaaaataaa tataggcaaa cgaaaataaa tgacctatta ttttgccagt
     2221 ttaggaagag attatgatga aaaacagcaa cgaattaaaa acgcttttat cagcatttac
     2281 ggagcaacag cggcaagcat ttcaatccga aaatgtactc agcttacttg cacaacgtag
     2341 ccaattttat gaccaactac tgcaagcact ttggcaagat tttggactcg ctcaatatga
     2401 acatattacc ctattagcgg taggggggta cggacgtgag gagatgtttc cgctttcgga
     2461 tctggatatc ttagtgttaa ctgaaaaacc gctagaagag gctattcagc agcgtttaaa
     2521 cgaactattt gccttgcttt gggacagcaa actgcaactt ggcaccagtg tgcgtacgct
     2581 cgaagaatgt attcagattg ggaaagcaga aatttcggtt gccactaata tgttggaagg
     2641 gcgattttta ttgggtaata aatcgctttg gctaagatta aaagaagcac tttatcagcc
     2701 ggatttctgg gatattaaag cgttttttag agcgaaaatg gcggaaaaaa atgagcgtta
     2761 tgcacgttat cataatacta gctataacct tgagccagat ctcaaacata gcccgggagg
     2821 attgcgagat ctgcatttaa tctcttggat tatgctacgg cattatggtg tatcttcttt
     2881 tgtcgatctc ctcaataaag ggctacttta cccggaagaa tataaggagc tgatcgcagc
     2941 acaagcggta ctttttcgga tgcgttttgc acttcatttg caactgaaac gttacgataa
     3001 tcgccttcgg tttgacagac aattacaact gagtgaaata ttaggctacc aaggcgaagc
     3061 aaatcaagcg gtggaattaa tgatgcgaga ttattttcaa gccacaaaat ctatttcgca
     3121 actgagccaa ttattgttaa acagttttga acaagcctat gtaaacgagt tgcaaaaaaa
     3181 tgcacaaaaa caaccgcttg atcagtattt ttatcggcaa gaacagcaat tgtttgtggt
     3241 cgaacgcaaa atatttaaaa cccaacccac aacaatgttg gatctctttt ttcatcttac
     3301 taatgaccct acgttaatag cgaatgccga caccttacgt cagttgagac tgtgtttaca
     3361 gcaacaacac gagcctatgt gctttttgcc tgaaatgcga gagaaattcg tacagctttt
     3421 tgctcaaccg aaagtcataa gccgagcaat agttccaatg caccaactgg gttttttaag
     3481 tgcttatcta ccgcaatgga aggggatcga agggctaatg caattcgacc tattccatat
     3541 ttatacagtc gatgagcata cggtcagagt catgttaaat ttagaaaatt ttagcgaaga
     3601 tccaagtcgg gcgtttttcc cactatgtca tcagctattt gcaacgcttt ctgagaaacc
     3661 gttgctctat cttgcggctc ttttccacga tattggcaag ggtagagagg gcgaacacgc
     3721 tcaagttggt gcgctggata tgcgagaatt tgcggaacta cacggttttt ctcaagcaca
     3781 aatcaatgat atggtttggc tggtggcaga gcatttgaca atgtcgatca ccgcacaaag
     3841 acgagatatc cacgatccgc aaatcatcag cgaatttgca aaaaaagtgc aaaatcagac
     3901 cgctttatct tcactacttt gtttaacggt tgcagatatt tgtgcaacca gtgagagcct
     3961 ctggaatgat tggaaagcct cgctgtttac ccaattatat caatttacgc tacagcaact
     4021 ggctcaaaat ttgaattatc aagcagtggc acaagagcat cgcttgcaag cattagaact
     4081 gatgaagttc gtgctttcag ctgatgagcg aatagcatta aacgcatttt ggcaaccttg
     4141 cccggagcgt tattttcttc accatacacc gactcagttg gtatggcacg ctctgcatta
     4201 tgtaaaaact cgccggttgc cgatggtttt aatcagtaat gaatatgccc gtggtggtac
     4261 agaaattttt gtatgttgcc aagatcaacc tcaactgttt gctcgaatcg cccaattact
     4321 cagccaaaaa cgcattagca ttcacgatgc acaaattatt actactgaaa atggtgtagt
     4381 gttagacagc tttattgtaa gtgagcgtga tggtgatgat gatttaaccg atgagagatg
     4441 tatgcaaatt cagcaggcga tgatgaaaat gttaagctta actgaaacag gcataaaatt
     4501 taccaaaaaa ccggtgaaac atttgtcatt taagcgtaaa actcggctgc gttttcttcc
     4561 tccgtcatca aagcaacaaa ccgaatttga actttttacc ttggatagag aagggttgtt
     4621 ggcacaaata ggctatattt ttaaccggct ccgattaaat ttaattaatg cgaaaattac
     4681 gactattggt gaaagggtag aggatttctt tgtggtcagt aatgatcaag gaaaagcttt
     4741 atcggaacaa gaacagttag aactaaaagc cattctaatt accgaactag atagtgaatg
     4801 aaaacaaccg cttggagtga agactgcaag cggtaaattt ttcaacgttt taggta
//
LOCUS       DANLXF010000181         4783 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.181, whole genome shotgun sequence.
ACCESSION   DANLXF010000181 DANLXF010000000
VERSION     DANLXF010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.181"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            40..2187
                     /locus_tag="PXD66_002018"
     CDS             40..2187
                     /locus_tag="PXD66_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDL6320282.1"
                     /translation="MKRLRYSYLALILGSALPLSAYADGYLSNDNIRQFDARFNFFLA
                     NPLPAPIAKADGRGEAIENLVVKYASDYLNDIYLKEDVIRFRRWQWQDLYTSLDLKTK
                     QKPDYLNTYRLQAEAYFINKQYQEALSQLDQILRRNPNDVHAMAMSVVASRVLENPNT
                     EAERLLALYQISPSIAQKVQQYLHFTDTMLAASYGSEPQTTMIPDTIAVFGQSPNPDG
                     TPSKGLLQRLEKTKEMAEKFPNAKIILSGGPVRYIYAEADVMKKWLIENGVDESRLLL
                     DDVARDTPGNAVGIIDFMKEYGGRKVLGIGTILHLPRAMSVLKSYADSIGYELEIDSA
                     GGGSPPNEKNKKGEALYTFVNVARAMGLFTLGDFENLLAKEQAEKEQREALEKAQQNY
                     LEKLVAERDQAVKNAENSANELEVARQALETAKQAHQQAEEELNKELSKTQEQLKQST
                     EALSRLIIKQNNPFSEHYVVQLNLMEQATYDLYRRLAEIEHTQDWNLWLSDSLSKYRL
                     KNRSTEDYHLLHIGLDHQVSHNWSIGGVFGISQGQIENRTHKVKGTSAGIYAGYRPTS
                     GLFWTNFFNYSHFTNQSGVTKRDNNAYSLSSEIGRNIVMSHQWQITPKLQLGWAKAGS
                     ARMFWAGTGLRLGKVIELNAIKLKPYADISYRFRNRHIDTLDSLSDRHRNVISIGIQA
                     VGNRHYFAIEGSMLHGDALKRSYGLHANYHYQW"
     gene            2200..3318
                     /locus_tag="PXD66_002019"
     CDS             2200..3318
                     /locus_tag="PXD66_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL6320283.1"
                     /translation="MKKIKTTLSALALSLSLISVNSWAIPADYLSQDNISQYDKAFNF
                     VLAKVLPAPKGKEDGRGAPIEHLVIQYAPDYVDDPYLKEDVLNFRRWYWQAMYASLDQ
                     KVKEKPTYLNTYRLQAEAYFVNKEYREALSQLDQILRQNPRDIHALAMTVLASRVLED
                     TEQETARLSALKQLAPEVAQRMTDFFAFTEQTLAASYGNEPQTAMIPDTIAVFGSTPN
                     KDGTPSPLLLDRLQKTKEMAQKFPQAKIIVSGGAVKTEFAEADVMAKWLSENGIEKDR
                     LLLDPVARDTPGNAVGIIKLMQTYNGKKVLGIGTLQHIPRATAVLKGYAQHIGYPIEM
                     DSVGGGKPATPGKAKIEALYTYVNVARSMGLFELGDFK"
     gene            complement(3379..4641)
                     /gene="rho"
                     /locus_tag="PXD66_002020"
     CDS             complement(3379..4641)
                     /gene="rho"
                     /locus_tag="PXD66_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDL6320284.1"
                     /translation="MHLTQLKNTPVSKLVEMGENQMGLENLARLRKQDIVFAILKQHA
                     KSGEDIFGQGVLEILPDGFGFLRSSDSSYLAGPDDIYVSPSQIRRFNLQTGDKIEGKI
                     RPPKEGERYFALLKVDSVNDDKPEASRSKILFENLTPLHANSRLKMERGNGSKEDLTA
                     RILDLAAPIGKGQRALIVAPPKAGKTVLLQNIAQSITHNHPECELIVLLIDERPEEVT
                     EMQRTVRGEVIASTFDEPATRHVQVAEMVIEKAKRSVEHKKDVVILLDSITRLARAYN
                     TVTPVSGKILSGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVDTGSKMDEVIFE
                     EFKGTGNMELHLSRRIAERRIFPAIDFNRSGTRKDDLLMAADELQKTWMLRKVLNPMD
                     EVDAIEWLIDKLMMAKTNEEFFEIMKRS"
BASE COUNT         1443 a          891 c         1077 g         1372 t
ORIGIN      
        1 ttataaatac ttcttcaccc aatataagga gaaatcataa tgaaacgctt gcgttattct
       61 tatcttgctt tgatattagg ttcagcctta cctttatcgg cttatgctga cggttattta
      121 agtaacgata atattcggca atttgatgcc cgttttaatt ttttcctcgc aaatccacta
      181 cctgcgccta ttgcaaaagc agatgggcgt ggtgaagcga ttgagaattt ggtggtgaaa
      241 tatgcatcag attatcttaa tgatatctat cttaaagagg atgttattcg ttttcgccgt
      301 tggcagtggc aagatctcta tacgtcattg gatttaaaaa cgaaacaaaa gcccgattat
      361 ttaaatactt accgattaca ggcggaagcg tactttatca ataagcaata ccaagaagca
      421 ttatctcaac tggatcaaat tctgcggcgt aaccctaatg atgttcatgc aatggcaatg
      481 agtgtggttg caagtcgggt attggaaaat cctaatactg aagccgagcg tttattagcg
      541 ctttatcaaa tctctccaag tattgcgcaa aaggtgcagc aatatctaca ttttacagac
      601 actatgcttg ctgccagcta tggtagtgag cctcaaacaa caatgatccc cgacaccata
      661 gcggtatttg ggcaatcgcc taacccagac ggtactccaa gcaagggatt attgcaacgt
      721 cttgagaaaa ccaaagaaat ggcagagaaa tttccgaatg caaaaattat tttaagtggt
      781 ggacctgttc gttatatcta tgcagaagcg gatgtaatga aaaaatggtt gatagagaat
      841 ggcgttgatg aaagcagact gttgcttgat gatgtggcac gagatactcc gggaaatgct
      901 gtcggtatca ttgattttat gaaggaatat ggaggtagaa aagtattggg gatcggtacg
      961 atcttacatt taccgagagc aatgtctgta ttgaaaagct atgcggattc cattggatat
     1021 gaattggaaa ttgattctgc cggaggcggt tctcctccta atgaaaaaaa taaaaaaggt
     1081 gaagctcttt atacatttgt taatgttgct cgtgcaatgg ggttatttac cttgggcgat
     1141 tttgagaatt tgcttgccaa agaacaagct gaaaaagaac aaagagaagc gttggaaaaa
     1201 gcacagcaga attatttaga aaaattggta gctgagcgag atcaagcagt aaaaaatgca
     1261 gaaaattctg caaatgagct ggaagtagcc agacaggcgt tagaaacagc aaaacaggct
     1321 catcagcaag ctgaagagga attgaataaa gaacttagta aaacgcaaga gcagttaaaa
     1381 cagagtacgg aagcccttag tcgtttaata ataaagcaga acaatccttt ttccgagcat
     1441 tatgttgtac agcttaattt gatggaacaa gcaacttatg atctgtaccg taggttagca
     1501 gaaatagaac atacacaaga ttggaattta tggctttcgg atagtctatc caaatatcgt
     1561 ttaaaaaatc gttcaaccga agactatcac ttattacata tcgggctaga tcatcaggtt
     1621 tcccataatt ggtctattgg tggagtattt ggtatcagtc aggggcaaat tgagaatagg
     1681 acgcataaag tgaaagggac atctgctggt atttatgctg gttatcgtcc tacaagtggt
     1741 ctgttttgga caaatttttt taattacagt cattttacta atcaatctgg tgtaacaaaa
     1801 cgagataata atgcatacag tctgagtagt gaaattggaa gaaatatcgt gatgagtcat
     1861 caatggcaaa ttacgccaaa gctgcaatta ggctgggcaa aagctggatc agcacgaatg
     1921 ttttgggctg ggacagggct acgtttagga aaagtgattg aactcaatgc aatcaaactt
     1981 aaaccttatg ctgatattag ctatcgtttt cgaaatcgtc atattgatac cttagatagt
     2041 ttatctgatc gtcatcggaa tgtaatcagt attgggatac aagcagttgg aaatcgccat
     2101 tattttgcaa tcgaaggatc aatgttacac ggggatgcat tgaagcgttc atacggatta
     2161 cacgcaaatt atcactatca atggtaaata ggagcctcta tgaaaaaaat caaaacaacc
     2221 ctttctgccc ttgctttaag cctttctttg ataagtgtaa atagctgggc gataccagct
     2281 gattatttga gccaagacaa tatcagtcaa tatgataagg cgtttaactt tgtattagca
     2341 aaagtactgc ctgcaccaaa aggtaaagaa gatggtcgag gagcgccgat tgagcattta
     2401 gttattcaat atgcgccaga ctatgttgat gacccttatc taaaagagga tgtgctgaat
     2461 ttccgccgtt ggtattggca agcgatgtat gcttcgcttg atcagaaagt taaagagaag
     2521 ccgacctacc ttaacactta ccgtttacaa gcagaggcgt atttcgtcaa taaagagtat
     2581 cgggaagcac tttctcagct cgaccaaatt ttgcgccaaa acccccgtga tattcacgct
     2641 cttgcgatga cagtattagc ttcaagggtg ctagaagata ctgaacaaga aacggctcgt
     2701 ctttctgcac tgaaacagct tgctcccgaa gtcgcacaac gaatgacaga cttttttgcc
     2761 tttaccgaac aaacgctggc ggcaagctac ggtaatgaac cacagacagc aatgatccct
     2821 gatacgattg ccgtttttgg ttctacgcca aacaaagacg gcacaccttc gccgctgttg
     2881 ttagatcgat tacagaaaac caaagagatg gcacaaaaat ttccgcaagc aaaaattatc
     2941 gtcagtggcg gtgctgtgaa aacggaattt gctgaggctg atgtgatggc aaaatggctt
     3001 agcgaaaatg gtattgaaaa agaccgtttg ctacttgatc ctgttgccag agataccccg
     3061 ggaaatgcgg ttgggattat caagctgatg caaacttata atggcaagaa agtattaggt
     3121 atcggtacgt tacaacatat ccctcgtgca acagcggttc tcaaaggtta tgcacagcac
     3181 attggttatc cgattgagat ggattccgtt ggtggtggca agccggcaac accgggaaaa
     3241 gccaaaatcg aggcacttta tacttatgta aatgtggcga gatcaatggg gttgtttgag
     3301 ttgggggatt ttaagtagtt actttattga cagctgctat tgataaaaat cccgcgttaa
     3361 gcgggatttt tttatgttct acgaacgttt cataatctcg aaaaactctt cgttagtttt
     3421 tgccatcatt aatttgtcga ttagccattc gatagcatct acttcgtcca ttgggttgag
     3481 gactttgcgt aacatccaag ttttttggag ttcgtctgct gccattaaga ggtcatcttt
     3541 acgagtacct gagcggttga agtcaatagc cgggaagata cggcgttcag caatacggcg
     3601 tgagaggtgt aattccatat tacccgtacc tttgaactcc tcgaaaatca cttcgtccat
     3661 ttttgatccg gtatccacaa gtgcggttgc gataatggtt aagctgccac cttcttccac
     3721 attacgggct gcaccgaaga aacgttttgg acgatgtaag gcgttagcgt ccacaccacc
     3781 ggaaaggatt ttgccggaaa ccggggtaac ggtgttgtag gcacgggcta aacgagtgat
     3841 ggagtctaat aaaatcacca catctttttt atgctctacc gagcgttttg ctttctcgat
     3901 taccatctcc gcaacttgaa cgtggcgggt tgccggttca tcaaaggttg aggcaattac
     3961 ttcaccacgt acggtgcgtt gcatctctgt tacttcttcc ggacgctcat caattaataa
     4021 tacgataagc tcacattcag gatgattatg ggtgatgctt tgtgcaatat tttgcagtaa
     4081 cacggtttta ccagcttttg gcggggcaac aattaacgct cgctgacctt taccgattgg
     4141 ggcggctaaa tctaaaatac gagcggttaa atcttctttt gatccattgc cacgctccat
     4201 ttttaagcga gaattggcgt gcagaggggt taagttttca aagagaattt tactgcgaga
     4261 ggcttccggt ttgtcatcat taactgaatc aactttcagt agggcaaaat agcgttctcc
     4321 ctcttttggt gggcgaattt taccttctat tttatcgcca gtttgtaagt taaaacggcg
     4381 gatttggctt ggagaaacat aaatatcatc agggccggct aaatatgagc tatctgatga
     4441 gcggaggaaa ccgaagccgt ccggtagaat ttcaagcacg ccttgcccga aaatgtcttc
     4501 accgcttttt gcgtgttgtt taagaatagc aaatacgatg tcttgtttac gtaaacgagc
     4561 cagattttct aagcccattt gattttcacc catttccaca agctttgaaa caggggtgtt
     4621 tttaagttga gtaagatgca taatttgaat gtaaaattgt gattaattaa gattggattt
     4681 gttgatattg attagatacg gccaaccgta aagatgtggc gaagaataac gcaataggag
     4741 acaaattgca aattttttat aaaaaataac cgcttgtttg caa
//
LOCUS       DANLXF010000182         4713 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.182, whole genome shotgun sequence.
ACCESSION   DANLXF010000182 DANLXF010000000
VERSION     DANLXF010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4713
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.182"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..549)
                     /locus_tag="PXD66_002021"
     CDS             complement(<1..549)
                     /locus_tag="PXD66_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320285.1"
                     /translation="MEGLIMGLQLIPHFPELAISRRTYLHDMPMYCFVSKNWIFSAKD
                     RAKREALGNLLACGGEMMKSFIFKRSLFSEIWFEDILLEDEEKELIRILYNKPEPEYQ
                     VEEKIDWEAREARRKQADYDKWQSYDERNPFHYQIFPTDTIAYAQQQVEKFIEDVDNF
                     LEWFYDDEVKRYILERNEIYQKK"
     gene            complement(1082..1408)
                     /locus_tag="PXD66_002022"
     CDS             complement(1082..1408)
                     /locus_tag="PXD66_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL6320286.1"
                     /translation="MLHSKGKILPKITLNEIETKERFAIALRKEMHAQYWTGKMVANA
                     LDVSEKTVKDWVAGKRLPSSLDLLALMRISSCVRQLVWDISKSEECYQDAEMLKQICF
                     VLLSNL"
     gene            1456..3087
                     /locus_tag="PXD66_002023"
     CDS             1456..3087
                     /locus_tag="PXD66_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL6320287.1"
                     /translation="MVTRTKNQPKNQAVSYIRMSTEHQEFSPDLQRCFIENYAKENGL
                     EIIREYIDEGKSGLTAEHRPQFLSMIQLVQSGQADFDHILVYDVSRWGRFMNIDESGH
                     YELICTYQGIKVHYCAEPFKGNDIASQIYKTMKRLSVGDYCRELGVKVFNGQKNLIQH
                     GFRQGGVAGFGLRRRLIDCQGNPKFDLQRGDRKSLQTDRVILVAGPKEEQEIVRQIYH
                     DFVYQHKTEQQIADSLNAQGIVTDRNTAWTKGVVHQILINEKYVGHNVWNKRSASKTR
                     EYKGKNPVEEWVRAENVFEAIVPQALFNAAQTIIHQRSARLSDDEMLEQLKLLLKEKG
                     KLSGLIIDEAENCPSSSAYSSRFGSLLNTYTLINYEPERDYHYVEINRLLRQQHKNFV
                     QQTLDTIIELGGVVLSDDVTDLVHINNEFTASIVLSRCRLTPSGSKRWLIRFDTSLNP
                     DITIAVRINESATEILDYYLLPTTEKVNEKLRLAESNPAELEIYRHDNLDRFFIMVER
                     ILVKEFIYAKRHHSNSSYRQNSGTQSQNAQSESLC"
     gene            3002..3895
                     /locus_tag="PXD66_002024"
     CDS             3002..3895
                     /locus_tag="PXD66_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HDL6320288.1"
                     /translation="MQNDTIQILPIDKIRVLNPRTRNQKVFADLVENIATIGLKRPIT
                     VTPSVDSAEYYDLLCGQGRLEACQVLGELFIPCRVVNANSEESYLISLVENIARRQHS
                     YMELLSGIKILNDRGYKPVDIARKIGFSKSHVNSILHLINAGEERLINAVEQGILPIT
                     IAISISRSDDQEVQKQLAELYTNGTLKSTDIAKIRNLIHRRNLAGKKANTGISGSIRS
                     SIYNPKTVVNIYKEETERQKMMIKQADYDEKQLSIILSCLNKLFEDKYFQLVLKSEHL
                     DDMPKDLSQRMLKQREGNIYA"
     gene            3888..>4713
                     /locus_tag="PXD66_002025"
     CDS             3888..>4713
                     /locus_tag="PXD66_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HDL6320289.1"
                     /translation="MPKHITYAFHNQLISLPIDEIIPLKKMDKNFEKSTKFQSILASI
                     REIGIVEPVVVYPEENNQYILLDGHSRVHALKKLGVDKVLCMISTDDEGFTYNKQINR
                     LTAVQENKMLMKTIERGVSDELIARTLNINLKALRQKMNMLNGITREVVDILANKQVS
                     KEIFRILRKMKPERQIEVAQMMIASNKFTLTYANMMLLSSRKEELVESHKSKTTNDDL
                     TDLSIMQKELERLKENYKISEEKLADLKMGLVVAKGYVKKLLNNAAVVDLLENDQSE"
BASE COUNT         1542 a          909 c          924 g         1338 t
ORIGIN      
        1 tttcttttgg taaatctcat tcctttctaa tatatagcgt tttacctcat catcgtaaaa
       61 ccattcaaga aagttatcaa catcttcaat gaacttttct acctgttgct gtgcatatgc
      121 tatcgtatcg gtagggaaga tttgataatg aaacggattg cgttcatcat aactctgcca
      181 tttgtcataa tcggcttgtt tacgccgcgc ttctcttgcc tcccagtcga ttttttcttc
      241 cacttgatat tctggctctg gcttattata caaaatacgg atgagttcct tttcttcatc
      301 ctcaagcaga atatcttcaa accagatttc agaaaatagg ctgcgcttga aaatgaaaga
      361 cttcatcatt tcccctccac acgccaataa gtttcccaat gcttcccgtt tcgctctatc
      421 ttttgccgaa aatatccagt ttttcgatac aaaacagtac attggcatgt catgcaaata
      481 ggttcttcgt gaaatggcta attcaggaaa atgaggaata agctgtaacc ccattattaa
      541 gccttccata ctgcacacga tcttacttaa ttgtccgtaa tcgcgtaaaa aagcaatgtc
      601 agtttttatc aaccacaacg ataggctgac taatatgaac ataaagatga gataatagat
      661 cggttttaag gctaaggcgc gccgacaaag ccacattaag agtggaatcg taacgagatc
      721 aaagaaaaat gatgagtcta tgatgatttc cttatgtgag ttgatgaata atcaggcatg
      781 aaggaaacct taaaaaggaa aagtcccttc attaccactt tttaaaacct tttaaggctt
      841 aaaaatcaaa agtcgtaatg aagggactat tgtccgtggg caaaaaggta ttagttatag
      901 ggggctttgt caagcgaaaa tttactgtgc attttctcgc caaaactgat ttaagccttg
      961 ataaaatctc gaaagtacct tgtcaagaaa aatcttttta gtggtttgat tgctagtttt
     1021 ttgagcaaaa ctatgctgta atgcagtaaa aatacagttt ctatctgtgt ttggaatcca
     1081 gttacaaatt ggagagtaat acaaagcata tttgcttgag catctcagca tcttggtagc
     1141 attcttctga ttttgaaata tcccaaacaa gctgccgtac acatgatgaa attctcatta
     1201 acgccaataa atctaggcta cttggcaatc ttttccctgc aacccaatct ttaactgttt
     1261 tctcactcac atctaatgca ttggcgacca ttttccccgt ccaatattga gcatgcattt
     1321 cctttcgtaa cgcaattgca aaccgctctt tcgtttcaat ctcattgagt gtaattttgg
     1381 gtaagatttt accctttgag tgcaacatat ttcctcccga ttcctttata ttggtgtaga
     1441 taaggagtca ttagaatggt tacacgtaca aagaatcaac ctaaaaatca ggcggtttcc
     1501 tacattcgaa tgtcaacgga acaccaagaa ttttcaccag accttcaacg ttgctttatc
     1561 gaaaattatg ctaaagaaaa tgggctagaa atcattagag aatacattga tgaaggaaaa
     1621 agtgggctaa ccgcagaaca ccgcccacaa ttcctttcaa tgatacaact cgtacaaagt
     1681 ggacaagccg acttcgatca tattttggtg tatgacgtga gtcgttgggg tcggtttatg
     1741 aatattgatg aatcggggca ttatgaactg atttgtacct accaaggcat taaagtccac
     1801 tattgcgccg agccattcaa agggaatgat attgcctctc aaatatataa aaccatgaaa
     1861 cgtttgtcgg taggagacta ctgtcgtgaa ctcggagtga aagtatttaa cggtcaaaaa
     1921 aatctgatcc aacatggatt tagacaaggt ggggtagctg gatttggatt gcgtaggaga
     1981 ttgattgact gtcaaggaaa ccctaaattt gatctccaac gtggagatag aaagagccta
     2041 caaaccgaca gagtgatttt ggttgcaggc ccaaaggaag agcaggaaat tgtccgacaa
     2101 atttaccatg attttgttta tcaacataaa acggaacaac aaattgctga tagcttaaat
     2161 gcacaaggca ttgttactga tagaaataca gcatggacga aaggcgtagt acatcaaatc
     2221 cttattaacg aaaagtatgt cggccacaat gtttggaata aacgtagtgc atcaaaaaca
     2281 cgcgaatata aaggtaaaaa ccccgtagaa gagtgggtgc gagctgagaa tgtatttgaa
     2341 gctatcgtgc cacaagcctt atttaatgca gcccaaacca ttattcatca acgctccgcc
     2401 cgattatctg acgatgaaat gttagagcaa ctcaagctac ttttgaaaga gaaagggaag
     2461 ctgtctggct tgatcattga tgaagccgaa aattgtccat caagtagcgc gtacagtagc
     2521 cgatttggaa gcctacttaa cacatatact ttaattaact atgaacccga acgggattac
     2581 cactatgtag agatcaaccg attactccgt cagcagcata aaaatttcgt acaacaaaca
     2641 cttgatacca ttattgaatt ggggggagtg gttttgagcg atgatgtaac cgatttagtc
     2701 cacatcaata atgaatttac cgcctcaatc gtattaagtc gttgtcgcct aacgccatca
     2761 ggaagcaaaa gatggttgat ccgatttgat accagtttaa atcccgacat aacaattgca
     2821 gtgcgcatca atgagagtgc cacagaaatt cttgattact acctgcttcc cacaactgaa
     2881 aaggtaaatg aaaaactacg attggctgag agtaatcctg ccgaacttga aatctaccgc
     2941 catgataatt tagatcgctt ttttattatg gttgaacgta ttttagtcaa ggagtttatc
     3001 tatgcaaaac gacaccattc aaattcttcc tatcgacaaa attcgggtac tcaatcccag
     3061 aacgcgcaat cagaaagtct ttgctgatct ggtcgaaaac attgcaacga ttggtctcaa
     3121 gagacctatt accgtaacgc catctgtcga tagtgctgaa tattatgatt tgttatgcgg
     3181 tcaaggtcgg cttgaggctt gtcaggtgct tggtgagcta ttcatacctt gtcgcgttgt
     3241 gaacgcaaat agtgaagaaa gctacttgat cagccttgtg gaaaatattg ccagacgtca
     3301 gcattcctat atggagctgc tttcaggcat caaaatactc aatgatagag gttataagcc
     3361 cgttgatatt gctcgcaaaa tcggtttctc aaaaagccat gtcaacagta tcttacacct
     3421 catcaatgct ggggaagagc gccttatcaa tgcggtagag caaggaattt tacccattac
     3481 catcgctatt tcgatttctc gttcagacga tcaggaagtt caaaaacaac tcgcggaact
     3541 ttacaccaat ggtacattga aaagcaccga tattgccaaa atacgtaacc tgatccaccg
     3601 tagaaatctt gctggtaaga aagccaatac aggtatttct ggaagtatta gatcatctat
     3661 ttataatccc aaaactgtgg tgaatatcta caaagaggaa acggaaagac agaaaatgat
     3721 gatcaagcaa gcggactatg atgaaaaaca actctctatt atcttgtctt gcttaaataa
     3781 actctttgag gacaaatact ttcaactcgt ccttaaatca gaacatttag acgatatgcc
     3841 aaaagattta agccaacgaa tgttaaaaca acgagaggga aatatttatg cctaaacata
     3901 ttacttatgc ctttcataat cagttaattt cgcttccaat tgatgagatt attccattaa
     3961 aaaagatgga taaaaatttt gagaaaagca ctaagttcca aagcattctg gcttcaattc
     4021 gtgaaatcgg gattgttgaa cctgtggtcg tctatccaga agaaaacaac caatatattt
     4081 tgttagatgg ccattctcgt gtacatgcac tcaaaaagct aggtgtggat aaggtgcttt
     4141 gtatgatttc taccgatgat gaaggcttta cttacaacaa gcaaattaac cgcttaaccg
     4201 ccgttcagga aaataaaatg ctgatgaaaa ccattgagcg cggggtttcg gatgaactga
     4261 tcgcaagaac actcaatatc aatctaaaag cactacggca gaaaatgaat atgcttaatg
     4321 ggatcacaag agaggttgtt gatatattgg ccaataagca ggtcagtaaa gagatatttc
     4381 ggattttgcg caagatgaag cctgaacgcc aaattgaagt ggcacaaatg atgatcgcca
     4441 gtaataagtt tacattgaca tacgccaata tgatgttgct cagttcacgc aaggaggaat
     4501 tagtcgaaag ccacaagtct aaaaccacga atgatgattt aaccgatctt tccattatgc
     4561 aaaaagagct agagcgactt aaagagaact ataaaatttc cgaagagaaa ttggcggact
     4621 taaaaatggg attggttgtg gcgaaagggt atgtcaaaaa actattgaat aatgctgccg
     4681 tggtagattt gcttgaaaat gatcagagtg aaa
//
LOCUS       DANLXF010000183         4683 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.183, whole genome shotgun sequence.
ACCESSION   DANLXF010000183 DANLXF010000000
VERSION     DANLXF010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4683
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.183"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..1304
                     /gene="argA"
                     /locus_tag="PXD66_002026"
     CDS             <1..1304
                     /gene="argA"
                     /locus_tag="PXD66_002026"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HDL6320290.1"
                     /translation="LALAQWFRQSTPYVNMHRGKTFVIMLDGDVIESPNFVNIINDIS
                     LLHSLNIKLVIVFGARPQINQLLKQNHIEPEYYHNIRVTNPQSLEYVKQAVGKVHYDF
                     FARLSLRLPHSEVINVVSGNMILAQPIGVVDGVDYTLSGKIRRINIESIKQQLAQNSI
                     VLLGPIAPSVTGEMFNLPFEDIATQVAIKLRADKLIGFCEEQGILDENGKPIPDYLPQ
                     DGETLLQQLIERGEYHTPKARFLQAAITVCREGVKRSHLLSYKEDGSLLQELFSRDGI
                     GTQFSMESSEQVRIANSSDIPGLLDLIRPLEQQGILVKRSREQLEMEITNYTIIERDG
                     VVIACAALNPYPEEKMAEMACVAVHPDYRDSSRGDILLENIQKRAVQLGMEKLFVLTT
                     RTTQWFQERGFRLAGAQDLPRYKRENYNYQRRSKVLILPLW"
     gene            1358..2245
                     /locus_tag="PXD66_002027"
     CDS             1358..2245
                     /locus_tag="PXD66_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL6320291.1"
                     /translation="MKKHQPIKGAMAIITAGVLFACINTLIPKLTSISPIDSNVIALV
                     QYLAAFIFLVPSMNNIGFFSSLKTEHFGQHCFRIFLSAIGIQCWTMALANQLPIWQGI
                     ALLMTSPLFVTIGSGLFLKEKVDKKRWIATAMGFVGAMIILEPWSDHFEMIALLPIAA
                     AFFWAGYSLMVKKLSRYDSPTTMVAYLFILITPFNLLIALTNLSPSGFSLPSWSDFGF
                     LILLGFFTALAQLAVAKAYSVADASYIQPFDFIKLPLNVLAGWLVFNWVPPGKLWLGA
                     AIIIAATIYITHAETKQSN"
     gene            complement(2324..2815)
                     /gene="ilvN"
                     /locus_tag="PXD66_002028"
     CDS             complement(2324..2815)
                     /gene="ilvN"
                     /locus_tag="PXD66_002028"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HDL6320292.1"
                     /translation="MRRTLAVLLENESGALSRVVALFSQRGFNIESLTVAPTDDPSLS
                     RMTIVAQGEEQVLEQIEKQLHKLIDVFKVSNLSAYEHVEREVLLVKVRATGSSRDELK
                     RMVDIYRGQIVDLTPKLYTIQLSGSTEKLNAFIEAVKEETTIVEIVRSGVISLSRGDK
                     NAL"
     gene            complement(2808..4529)
                     /locus_tag="PXD66_002029"
     CDS             complement(2808..4529)
                     /locus_tag="PXD66_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HDL6320293.1"
                     /translation="MKKLSGAEMIVQSLKDEGVEYVFGYPGGSVLDIYDAIHTLGLNH
                     VLVRHEQGAVHMADGYARSTGKVGCVLVTSGPGATNTITGILTAYTDSVPLVILSGQV
                     PSSLIGTDAFQESDMIGISRPVVKHSFLVKNTEEIPEIIKKAFYIASTGRPGPVVIDI
                     PKDVVNPLNKYPYEYPQEVSMRSYNPTVQGHKGQIKKALKALLVAKRPVLYVGGGVIS
                     AEGSPEITEFAQKLNLPVTCSLMGLGAYPASDKQYLGMLGMHGTFEANNAMHESDLIL
                     GIGVRFDDRTTNNLAKYCPNAKVIHVDIDPASISKTVQAYIPIVGSAKDVMEEFLSLL
                     GEENLAKNQVDLTAWWQQIDAWKARNCLAFEENSDVIKPQQVIRMIHKLTNGEAYVAS
                     DVGQHQMFAALHYGFDKPRRWINSGGAGTMGFGLPAAMGVKFAHPDATVVCVTGDGSI
                     QMNIQELSTAKQYDTPIVIVSLNNRFLGMVKQWQDIIYSGRHSQVYMNSLPNFAKLAE
                     AYGHVGIQIDHPSELEAKLTEAFSIKDKLVFVDVLVDETEHVYPMQVRGGAMNEMMLS
                     KTERTDA"
BASE COUNT         1333 a          968 c          927 g         1455 t
ORIGIN      
        1 aactcgcatt agctcaatgg tttaggcaat ctacacctta tgttaatatg cacagaggca
       61 aaacctttgt gattatgctt gatggcgatg ttatcgaaag cccgaatttt gtcaatatta
      121 tcaatgacat cagtctcctg cacagcctga atatcaaatt agtgattgta tttggggcaa
      181 gaccgcaaat taaccaattg ttaaaacaaa atcatattga gccggagtat taccacaata
      241 tccgtgtgac taatccacaa tcgctagaat atgtgaaaca agcggtaggg aaagttcatt
      301 atgatttctt tgcccgcctt tctttgcgtt taccccattc tgaagtgatt aatgtagtca
      361 gtggtaatat gattttagct cagccgattg gcgtggtgga tggggtagat tatacactaa
      421 gcggtaaaat ccgtcgtatt aatattgaga gcattaagca gcaactggca caaaattcga
      481 ttgtattact tggtccgatt gccccgtctg ttaccggtga aatgtttaat ctgccgtttg
      541 aagatattgc cacacaggtt gcaattaaac ttcgtgccga taaattaatc ggtttttgtg
      601 aagagcaggg gattttagat gaaaacggca agccaattcc agattatctt ccgcaagatg
      661 gcgaaacctt gttacagcag ttaatcgagc gtggcgaata tcacaccccg aaagctcggt
      721 ttttgcaggc ggcgatcacg gtatgccgag agggagtgaa acgctcgcat ttattaagct
      781 ataaagaaga tggctcattg cttcaagagt tattctctcg tgatggtatt ggcacacagt
      841 tctcaatgga aagctcggaa caggtacgta ttgctaattc cagtgatatt ccgggattat
      901 tggatttaat ccgcccactt gaacagcagg gcattttagt caaacgctct cgagaacaat
      961 tagaaatgga aatcacaaac tacaccatta ttgagcgtga tggtgtggtg attgcttgtg
     1021 cggcacttaa cccgtatccg gaagaaaaaa tggcggaaat ggcgtgtgtg gcggttcacc
     1081 ctgattatcg tgattcctca agaggggata ttctactgga aaatattcaa aaacgggcag
     1141 tgcaactggg aatggagaaa ctctttgtgc tgactacaag aaccacacaa tggttccaag
     1201 agcgaggatt taggctggca ggtgcccaag atttacctcg ctataaacgg gaaaattaca
     1261 attatcaacg tcgttctaaa gtattaattt taccactttg gtaaaatatt caatgttttt
     1321 gaccgcttgt taccaagaga actataaaag gagaactatg aaaaaacatc aacccatcaa
     1381 aggggctatg gcaattatta ctgccggtgt gttatttgcc tgtatcaata ccttaattcc
     1441 caaattaacg tccatttcac cgattgactc gaacgtgatt gcattggtgc aatatttagc
     1501 tgcctttatt ttcctcgttc ccagcatgaa taatatcggt ttcttctctt cattaaaaac
     1561 cgagcatttt gggcaacact gtttccgtat ttttctatct gcgattggta ttcaatgttg
     1621 gacaatggca ctcgctaacc aattacctat ttggcaggga attgcgttat tgatgacttc
     1681 tccattgttt gtgaccatcg gctccggtct gtttttgaaa gagaaagtgg ataaaaagcg
     1741 ttggattgca accgcaatgg ggtttgttgg ggcgatgatt attctagagc cttggagcga
     1801 tcattttgaa atgattgcgt tactgccgat tgccgcggct tttttctggg caggttattc
     1861 gctaatggtg aaaaaactct ctcgttacga tagcccaact acgatggtcg cttacctgtt
     1921 tattctaatt acgcctttta atttgttgat tgcactcacg aaccttagcc ctagtgggtt
     1981 tagtctgcct tcttggagcg atttcggctt tttaatctta cttggtttct ttacggcgtt
     2041 agcgcaactg gcggtagcca aagcctacag tgtagcagat gcttcctaca ttcagccgtt
     2101 tgattttatt aaattacctt tgaacgtatt agccggttgg cttgtattta actgggtgcc
     2161 accgggtaaa ttatggctag gagctgcgat tattattgct gcgaccattt atattactca
     2221 cgctgaaact aaacagagta attagcaaca aattcacgct acataaaaat ccctttatct
     2281 caaagataaa gggatttgca aattttaagc taaaattgac cgcttacaga gcgtttttat
     2341 cgccacgaga tagactaatc acgccagagc gaacaatctc tacaatcgtt gtttcttctt
     2401 taactgcctc aataaatgca ttgagcttct cggtagaacc ggataattga atggtgtaaa
     2461 gtttcggtgt taaatctacg atttgcccac ggtaaatatc taccatacgt ttcagttcat
     2521 cacgagatga acctgtagct cgcactttta ccaataacac ttcacgctca acgtgttcat
     2581 aagcacttaa gttactcact ttaaacacat caatgagttt gtgtagttgc ttttcgattt
     2641 gttctaacac ctgttcttcg ccttgcgcga caattgtcat acgggaaagg cttggatcat
     2701 cagtaggggc aacggtcaag ctttcaatgt taaaaccacg ctgcgagaaa agggcaacta
     2761 cacgagataa tgcacctgat tcattttcta ataatacggc taatgttcta cgcatctgtt
     2821 ctctccgttt tacttaacat catttcattc attgctcctc cacgcacttg cattgggtaa
     2881 acatgttccg tttcatcgac caatacatca acaaatacaa gcttatcttt aattgagaat
     2941 gcctcagtta attttgcctc caattcagaa gggtggtcga tttgaatccc aacgtggcca
     3001 taagcctctg ctaatttagc aaagttaggt agagaattca tatacacctg tgaatggcgg
     3061 ccagagtaaa taatatcttg ccactgtttc accataccta agaaacggtt atttaagctg
     3121 acaatcacaa tcggtgtgtc gtattgcttt gcggtggaaa gttcctggat attcatttgg
     3181 atactaccat caccggttac acaaacaaca gtggcatcag ggtgggcaaa ttttaccccc
     3241 atcgcagcag gtaagccaaa gcccattgtg cctgcaccgc ctgaattaat ccaacgacgt
     3301 ggtttatcga acccataatg caatgcagca aacatttgat gttgtccaac atctgaggca
     3361 acataagcct ccccattggt caacttgtgg atcattctaa tcacttgttg tggcttaatc
     3421 acatcactgt tttcttcaaa ggctaagcaa ttacgagctt tccaagcatc aatttgttgc
     3481 caccaagcgg ttaaatctac ctgatttttt gcaagatttt cctcacctaa taaagataaa
     3541 aattcttcca ttacatcctt agcactgcct acaattggaa tataagcttg tactgttttg
     3601 gaaattgagg ctggatcaat atccacgtgg atcacttttg cattcgggca atattttgct
     3661 aagttatttg ttgtacgatc atcaaaacgt accccaatac ctaaaatcaa atcactttcg
     3721 tgcattgcat tatttgcctc aaaagtgccg tgcatgccta acatacctaa atattgctta
     3781 tcagaggcag gataagcacc taatcccatt aatgaacagg ttacaggtag atttagcttt
     3841 tgtgcaaact ctgtaatttc tggcgaaccc tctgcactaa ttacaccacc gcctacataa
     3901 agcactggac gttttgctac taatagtgct tttaatgctt ttttaatttg ccctttatgc
     3961 ccttgaactg ttgggttata agaacgcatt gatacttctt gcggatattc atagggatat
     4021 ttatttaatg gattgactac atctttagga atatcaataa ctaccgggcc tggtcgaccg
     4081 gttgatgcaa tataaaaggc ttttttgata atttcaggaa tttcttctgt attttttact
     4141 aagaaactgt gttttacaac cggacgagaa atgccgatca tatcactttc ttggaatgca
     4201 tctgtcccaa ttaaacttga tggaacttgg cccgacagta ttactaacgg aacagagtcg
     4261 gtataagcag ttaaaatacc agtaattgta ttcgttgctc ccggacctga agtcactagc
     4321 acacagccga ctttacctgt tgaacgggca tagccatcag ccatatgtac agcaccttgc
     4381 tcgtgtcgaa ctaaaacgtg attcaatcca agtgtatgaa ttgcatcata aatatcgagt
     4441 acagagccac ccggatagcc gaaaacgtat tctacgcctt catcctttaa ggactgtaca
     4501 atcatttctg caccagaaag ttttttcatt gaatcctcca acaaaatagc attttcttct
     4561 ctaagatggt tggattttgc agtaaaattt tattgatgtc tagttcttat tatgggatca
     4621 aatgctagat ttgctagatt tatattcttt tttatcttaa aaagttggga ttttataaaa
     4681 ata
//
LOCUS       DANLXF010000184         4664 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.184, whole genome shotgun sequence.
ACCESSION   DANLXF010000184 DANLXF010000000
VERSION     DANLXF010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4664
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.184"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            77..1267
                     /locus_tag="PXD66_002030"
     CDS             77..1267
                     /locus_tag="PXD66_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HDL6320294.1"
                     /translation="MFSHIQAAPADSILGLGEAFKADSREGKINLGIGVYMTDEGKTP
                     IVKAVKEAEKRLLETENSKNYLTIDGVQAFNAATQALLFGEGAEVITSGRAKTAQSLG
                     GTGALRIAAEFIKRHTNAKNVWISTPTWPNHNGIFDAVGVNVKGYRYYNKETNGLDWE
                     NLIADLSQAEAGDVVLLHGCCHNPTGIDPTPAQWEQLAALSAEKGWLPLFDFAYQGFA
                     NGLEEDAYGLRAFVKNNRELLVASSFSKNFGLYNERVGAFTLIADNADDANRAFTQIK
                     SIIRVLYSNPSAHGASAVAVALADPDLKAQWIAELDEMRNRIKEMRSQLVQKLKEKGA
                     NKDFSFITEQNGMFSFSGLTPEQVDKLKDEFAIYAVRSGRINVAGITSKNIDALADAI
                     VKVL"
     gene            complement(1374..2675)
                     /gene="folC"
                     /locus_tag="PXD66_002031"
     CDS             complement(1374..2675)
                     /gene="folC"
                     /locus_tag="PXD66_002031"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDL6320295.1"
                     /translation="MNTLSIPKATDSLETWLSYLEQAHSKPIDMGLDRIRKVATELDL
                     LKPAPYVITVAGTNGKGSTCRLLEVTLLKAGFRVGVYSSPHLIHYNERVRINGEVLPD
                     ADHVQAFAKINAKKTASLTYFEFSTLSAFELFKQNQLDIVILEVGLGGRLDATNIIDP
                     SLAVIISIDIDHVAFLGDNREDIGREKAGIFRENIPVVIGEPDCPHSIKQIADSLNCH
                     AFYRGKDWQFEIKNNLFHWYSEEKSFTDLPIPLIPIPNAGTALAVLMQLPFEIDEKII
                     RQALNEAQMTGRFQTLTDTDFTHFSQPKPAAKVIIDVGHNPHAARYLADKLRELKQPN
                     QKIYAVFSALEDKDLSGIVEPLANIIDEWHCAGLDCWRGQTGEAVLAKLVNVLPNSTA
                     CSYENVAQASRVLFETANENDIVLVFGSFHTVADFMLWLET"
     gene            complement(2685..3581)
                     /gene="accD"
                     /locus_tag="PXD66_002032"
     CDS             complement(2685..3581)
                     /gene="accD"
                     /locus_tag="PXD66_002032"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HDL6320296.1"
                     /translation="MSWLDRILGRDSSSSSNAKSKVPEGVWTKCTSCEQVLYSEELQR
                     NQQVCPKCDHHMRIDARTRLLNLLDKDSAVEIAADLEPEDVLKFKDLKKYKDRLTAAQ
                     KQTGEKDSFIVLAGTLHNMPVVTASFNFEFMGGSMGSVVGAKFVKAAEKALAEKIPFI
                     CFSASGGARMQEALFSLMQMAKTSAILAKMKEQGVPFISVLTDPTLGGVSASLAMLGD
                     VNIAEPKALIGFAGPRVIEQTVREKLPEGFQRAEFLLEHGAVDMIVHRKEMRDTLSRL
                     CAKLTNQPTPFKVAELVVENAE"
     gene            3890..4664
                     /gene="mazG"
                     /locus_tag="PXD66_002033"
                     /pseudo
     CDS             3890..4664
                     /gene="mazG"
                     /locus_tag="PXD66_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600778.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
BASE COUNT         1409 a          956 c          961 g         1338 t
ORIGIN      
        1 tgctcttgct aaatctgcaa tttaatcgta taaattcact gtttattttt cattttgatg
       61 aggaactcca atgtcaatgt ttagtcacat tcaagctgct ccggcagact caattttagg
      121 cttaggtgaa gcgtttaaag cagatagccg tgaaggtaaa atcaaccttg gtatcggtgt
      181 ttatatgacc gatgaaggca aaaccccgat tgtcaaagcg gtaaaagaag cagaaaaacg
      241 tttattagag acggaaaaca gtaaaaatta tttaaccatt gacggtgttc aagcattcaa
      301 tgcggcaacg caagcgttat tatttggcga aggggctgaa gtgattacct caggtcgtgc
      361 taaaactgca cagagtttag gtgggacagg ggcattacgc attgcggcgg aatttatcaa
      421 acgtcatacg aatgcgaaaa atgtatggat ttcaaccccg acttggccaa accataacgg
      481 catttttgat gcagtaggcg tgaatgtaaa aggctatcgc tactacaata aagagactaa
      541 cggtttagat tgggaaaatt taattgccga tttaagccaa gcggaagccg gagatgtggt
      601 gttattgcac ggttgctgcc acaacccgac cggaattgac ccaacacctg cacaatggga
      661 acagttagca gcattatctg ccgaaaaagg ctggttgccg ttatttgact ttgcttatca
      721 aggctttgca aacggtttag aggaagatgc ttacggttta cgagcttttg tgaaaaataa
      781 ccgtgaattg ttggtcgcaa gctcattctc gaaaaacttt gggttatata acgagcgtgt
      841 cggtgcattt accctaattg cagataatgc tgatgatgcc aaccgtgctt tcactcaaat
      901 caaatcgatt attcgtgtac tttactctaa cccatctgct cacggtgcaa gtgcagtagc
      961 agttgcattg gctgatcctg accttaaagc acaatggatt gcggaattag atgaaatgcg
     1021 taaccgtatt aaagaaatgc gtagccaatt agtgcagaaa ttaaaagaaa aaggggcaaa
     1081 caaagacttc tctttcatta ctgagcaaaa tggtatgttc tcattcagtg gcttaacacc
     1141 ggagcaagtg gataagttaa aagatgagtt tgcaatttat gctgtgcgtt caggcagaat
     1201 caatgtagca ggcattacca gcaaaaacat tgatgctctt gccgatgcga ttgtgaaagt
     1261 gctttaatta aaccttaaag atatatagag gcataagcct gagtgcttgt gcctcttttt
     1321 tatcttttag gtacaagtgt ggacacttgc accaacgttg attattcgta gaactaggtt
     1381 tccaaccaaa gcataaaatc tgctaccgta tgaaatgagc cgaacaccag cacaatatca
     1441 ttttcgttgg ctgtttcaaa caatactcgg cttgcctgtg ccacattttc atagctacaa
     1501 gcggttgaat ttggcaaaac gtttaccaat ttcgctaaca ccgcctcgcc tgtttgtcca
     1561 cgccaacaat ctagccctgc acaatgccat tcatcaataa tattagctaa tggctcgaca
     1621 ataccggata aatccttatc ttctaacgca ctaaacaccg catagatttt ttgatttggt
     1681 tgctttaact cccgcagttt gtcagctaaa taacgtgctg cgtgtgggtt atgccctaca
     1741 tcaataatta cttttgcagc cggtttaggt tgagaaaaat gagtaaaatc ggtatccgtc
     1801 agggtttgaa aacgccctgt catttgagct tcatttaagg cttgtcgaat aatcttttca
     1861 tcaatttcaa atggcagttg cattaatact gctaatgcgg tgccggcgtt tggaatcgga
     1921 ataagcggga ttggtaaatc ggtaaaggat ttttcttcag aataccaatg aaacagatta
     1981 ttcttaattt caaactgcca gtctttaccc cggtaaaaag cgtgacaatt taacgaatca
     2041 gcaatctgtt taatcgaatg agggcaatca ggctcgccga tgacaaccgg aatattttca
     2101 cggaaaatgc cggctttctc acgcccgatg tcttcacggt tatcgcctaa aaatgcaacg
     2161 tggtcgatat caatcgagat aatcacggca aggcttggat caataatatt agtggcatct
     2221 aatcgcccac ctaatccgac ttctaaaatc acaatatcga gctgattttg tttgaatagc
     2281 tcaaatgcag acagcgtact aaactcaaaa taagtgagtg aagcggtctt ttttgcatta
     2341 atttttgcaa atgcttggac gtggtcggca tcaggtaata cttcgccgtt aattcgcacc
     2401 cgttcattat agtgaattaa gtgcggtgaa gagtaaacac caacccgaaa tccggctttt
     2461 aataaggtaa cttctaacaa acggcaggtc gaacccttgc cgttagtacc ggcaacggta
     2521 atcacataag gggcaggctt gagtaaatcc agctctgttg cgaccttacg aatacgatct
     2581 aagcccatat caatcggttt agaatgggct tgttctaaat aggaaagcca cgtttccaaa
     2641 gaatccgtgg ctttaggtat gcttaatgta ttcattcaga tattttattc tgcgttttct
     2701 acaaccaatt ccgccacttt aaacggcgtt ggttgattgg ttaattttgc acaaagacgg
     2761 gaaagggtat cacgcatctc tttacgatgc acgatcatat caactgcccc gtgttctaat
     2821 aagaactcgg cacgttggaa accttccggc agtttttcac gtacggtttg ctcaattaca
     2881 cgaggaccag caaagccgat tagggctttt ggctcagcaa tattcacatc gcccagcatt
     2941 gccaaacttg cggaaacccc acctaaagtt gggtcggtta atacagaaat aaacggtacg
     3001 ccctgctctt tcattttggc taaaatcgca ctggtttttg ccatttgcat taaagagaat
     3061 aacgcctctt gcatacgagc cccgcccgat gccgaaaaac agataaacgg aattttttca
     3121 gctaaagctt tttccgcagc tttcacaaat tttgcaccta ctacagaccc cattgaaccg
     3181 cccataaact caaagttaaa ggaggccgta accaccggca tattatgcaa tgtaccggca
     3241 agcacgataa aagaatcttt ctcaccggtt tgtttttgcg cggcagttaa acgatcttta
     3301 tatttcttta aatctttaaa tttcaacaca tcttccggct ctaagtcggc agcgatttcg
     3361 accgcagaat ctttatctaa caaattcaat aaacgggtgc gggcatcaat acgcatatga
     3421 tgatcgcatt ttggacaaac ctgttggttg cgttgtaatt cttcgctata gagaacttgt
     3481 tcacaacttg tgcattttgt ccatacacct tccggcactt ttgattttgc attgcttgat
     3541 gatgaagagt ctctacctaa aattctgtct aaccagctca ttattattcc ttttggattg
     3601 agttgttggg ttcactaaat ggttgctatt aaagcataat ttatcggttt ttgatatgtt
     3661 ttcttgacat atcagatcac taactatgtg tataagttcg ataaaaaaac gcaatcagaa
     3721 gaatgattaa catcaccaca atttgaatta agcgtttttt ggttaatttt tccgctgcca
     3781 ttgctctgct catacttaat aaatttccgc attataagcg aaaaagcacg ggctatgtag
     3841 aatttgcaaa attttgatca aaaataaccg cttgtaaaaa ggaaatacta tggcaaccat
     3901 tgaacaattt cttgaggttg taaaccaact tcgtcaccct gaacacggct gcccgtggga
     3961 tctcaaacag aattttgaca cgatgttccc cacttattgg aagaaaccta cgaagtagcg
     4021 gaagcgattc acacccaaga tcgcactgcc ttgcgtgaag agttaggcga tttattactg
     4081 caagtggtat ttttaagcca attagcgaaa gaggaaggca gtttcacttt tgatgatgta
     4141 gtaacggata ttcacgataa actgatttac cgccaccctc acgtttttgg tgatgtgaaa
     4201 gctactaaca gcgaagaggc cttacaaagt tgggaagcac aaaaagcgaa tgatgcaaaa
     4261 caccgtagcc aagattctat ttttagatga tttacctttt gctttgcctg ctcttacccg
     4321 tgcaaataag ctacaaaaac ggtgtgcaaa agttggattc gattgggata accctcacga
     4381 tgtgctggca aaagtcgaag aagaactcga tgaagtcaaa gcagaaatcg cacaatatcc
     4441 ggctcgctcc atcgagcttg ctgaagaatt aggcgatctc ttgtttgcga ttgtaaattt
     4501 atgccgccat tatcataccg atgccgagga aattttacgc aatgcaaatc taaaatttga
     4561 acatcgcttt cgaaaagttg aaaaatctgt gaaaaaatcg ggaagaactg taaaagaatg
     4621 taaattagaa gaactagatg taatttggca caaaataaaa gtgg
//
LOCUS       DANLXF010000185         4609 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.185, whole genome shotgun sequence.
ACCESSION   DANLXF010000185 DANLXF010000000
VERSION     DANLXF010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.185"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            18..281
                     /locus_tag="PXD66_002034"
     CDS             18..281
                     /locus_tag="PXD66_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL6320297.1"
                     /translation="MSTLEDIKKTAKDWHRADIIAALHKAGWSLRQLSLKHGYSQSST
                     LKNAVDRPWLKGERIIAEAIGISPEEIWPERYAQRNHKKFADR"
     gene            297..827
                     /locus_tag="PXD66_002035"
     CDS             297..827
                     /locus_tag="PXD66_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL6320298.1"
                     /translation="MSENSLKTHYSAKELAELSLACLPKTKQGVLYQAKKQGWTTHKK
                     VGVGGEILEFALSNLPQEIQTEIRSRFMSAVVEAKPKKLPAVRAEVDVGNLTTKQREI
                     ADARMALVAYVLELEGSMSRIKAITYLCNLAKQGEMPPHLVELVAVANAKKTAKRTIV
                     PIFSPIMPDFPLDSRD"
     gene            complement(843..1970)
                     /locus_tag="PXD66_002036"
     CDS             complement(843..1970)
                     /locus_tag="PXD66_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL6320299.1"
                     /translation="MNKLKSINLQVYIMLIAIVVIMSFFSIATDGAYLSARNISNLLR
                     QTSITGILAIGMVFVIISAEIDLSVGSLMGLLGGFAAISNVWWGWPLSLTIIVTLILG
                     VMLGAWSGWWVAYQKVPSFIVTLAGMLAFRGVLVGLTNGTTVSPISNEMTTIGQGYLP
                     DMVGMILGTIGIFCFIVWGSYQRKARQKLNLTVPSMSKDTLKYGLVAIIVLGAIYLLN
                     DYRGVPFPVLVLVILAIAGTFLARKTAFGRHIYAIGGNIDAARLSGISVEKVKLAIFA
                     LNGLLVAIAGLILSSRLGAGAPSAGQNAELDAIAACVIGGASLAGGIGSIYGVVIGAF
                     IIALLDNGMSMLDVPTFWQYIVKGGILLLAVWVDTISKKKA"
     gene            complement(1974..3488)
                     /gene="xylG"
                     /locus_tag="PXD66_002037"
     CDS             complement(1974..3488)
                     /gene="xylG"
                     /locus_tag="PXD66_002037"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HDL6320300.1"
                     /translation="MAKLLEMKNITKKFGEVVALNNISISLETGEILSLCGENGSGKS
                     TLMKVLCGIYPAGDYEGEIYFSGEKLIARNIKDTEEKGISIIHQELTLVKNMSILENM
                     FLGNEITKSGITDDNEMYLRCKTLLEQVQLDIDPNTKVGELGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEKETEILLSLIKDLQAHNIACVYISHKLNEVKAISDKICVIRDG
                     EHIGTRSAEGMSEDDIITMMVGREITSLYPHEPHNIGEEVLRVENLTAWHPTNTHIKR
                     VDNANFVLRQGEILGIAGLVGSGRTEMAQCIFGSYQGKYQGDIFLHKQPIKIKNCAQA
                     IENHIVMVPEDRKKHGIIPIMGVGKNITLSSLPQFCFGKKVINEPLEETIINQSIAKL
                     KVKTSSPELAIGRLSGGNQQKAILAKCLLLNPKILILDEPTRGIDVGAKYEIYKLINQ
                     LAKEGMAIIVISSELPEVLGISDRVLVMHQGKIKADLINQDLTQEKVMEAALKE"
     gene            complement(3555..4556)
                     /gene="xylF"
                     /locus_tag="PXD66_002038"
     CDS             complement(3555..4556)
                     /gene="xylF"
                     /locus_tag="PXD66_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005576719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL6320301.1"
                     /translation="MKLKSKLLAVATATLMVLSNASVAKDLKIGMSIDDLRLERWQKD
                     RDIFVNKAESLGAKVFVQSANGDATAQISQIENMLNKDIDVLVIIPFNGEVLSNVIAE
                     AKKEGVKVLAYDRLINNADVDFYVSFDNEKVGELQAKSIIGQKPEGNYFLMGGSPVDN
                     NAKLFRKGQMKVLQPLIDSGKIKVVGDQWVDSWLAEKALQIMENALTANKNNIDAVVA
                     SNDATAGGAIQALSAQGLSSKVAISGQDADLAAIKRIIAGTQTMTVYKPITNLADKAA
                     EIAVALGKDEKVEMNAQLNNGMKEVPAYLLEPVVVTKENIDDTVIKDGFHTKDAVYK"
BASE COUNT         1359 a         1020 c          872 g         1358 t
ORIGIN      
        1 gcgaaaggag ttatctaatg agtacattgg aagatattaa aaaaacagcg aaagactggc
       61 atagggccga catcatcgct gctttacata aagcgggttg gtcgcttaga caactctcat
      121 tgaagcatgg ctacagtcaa tccagcacct tgaaaaacgc tgtagatcgt ccgtggttaa
      181 agggtgagcg tattattgcc gaagcaatcg gtatatcacc cgaagaaatt tggcctgaac
      241 gttatgctca acgaaaccat aaaaaatttg ccgacagata gcttaaagag gtttttatga
      301 gtgaaaacag tctaaaaaca cactattcgg caaaagaatt ggctgagtta agtttagcct
      361 gtttacctaa aacaaagcaa ggagttttgt atcaggcaaa gaaacaagga tggactactc
      421 ataaaaaggt tggagttggg ggagaaattt tagaatttgc actctcaaac ctccctcaag
      481 aaatccaaac cgaaatccgc tcccgcttta tgtcggcggt ggtggaagcc aagccgaaaa
      541 agttaccggc agtgcgtgcg gaggtggatg tgggaaacct gaccaccaaa cagcgagaaa
      601 ttgctgatgc acgaatggca ctggtggcgt atgtgttgga actggaaggc tcaatgagcc
      661 ggattaaagc gataacctac ctttgcaacc ttgccaaaca aggcgaaatg ccaccgcact
      721 tggtggagct ggttgccgta gcaaatgcta aaaagacggc aaaacgcaca atagttccca
      781 tttttagccc gattatgccg gatttcccgt tggatagtcg agattgaccg cttgttatcc
      841 aattaggctt ttttcttgct gatggtatct acccaaactg caagcagtag aattccacct
      901 ttaacgatat attgccagaa ggttggcaca tcaagcatac tcataccgtt atccagcaaa
      961 gcaataataa atgctccaat aactacgcca taaatgctac cgataccgcc ggctaaactg
     1021 gctccaccaa tcacgcaggc ggcaatcgca tctaattcgg cattttgccc ggcggaaggt
     1081 gcgccggctc caagacgtga gcttaaaatc aagcctgcaa tggcaactaa taagccgttt
     1141 aaggcaaaaa tcgcaagttt gactttttca actgaaatcc ccgagaggcg agcggcatca
     1201 atgttgccac caatcgcata aatgtgacgc ccgaaggcgg tttttctggc caagaatgtg
     1261 ccggcaatgg ctagaatcac caacaccaat accgggaatg gcacaccacg gtaatcattg
     1321 agtaggtaga tcgcacctaa tacgataatt gcaactagtc cgtatttcag cgtgtctttc
     1381 gacatagaag gcacagtaag attgagtttt tgtctggctt tgcgttggta gctaccccaa
     1441 acaataaagc aaaatatccc gatagtacct aaaatcatac ccaccatatc aggcaaatag
     1501 ccttgaccaa tggttgtcat ttcattgcta atcggtgaaa cggttgtccc gttggttaag
     1561 ccgaccaata caccacgaaa cgccagcata ccggcaagag ttacaataaa ggacggcact
     1621 ttttgataag caacccacca accgctccaa gcacccagca tgacgcctaa aattaaagta
     1681 actataatag ttaacgagag cggccagccc caccatacat tactgatggc ggcaaatcca
     1741 ccgagtaagc ccattagcga gccaaccgat aaatcaattt ctgcggaaat aatcacaaat
     1801 accataccga tggcaagaat accggtaatg gaggtttggc gtaacaggtt ggagatattt
     1861 ctggcactta aataagcacc gtcagttgca atagagaaaa agctcataat cacaacaatt
     1921 gcaatcagca taatataaac ttgtaaatta atcgatttta acttattcat aggttactcc
     1981 ttgagtgccg cttccattac tttttcttgg gtaaggtctt ggttaatcaa atctgcttta
     2041 attttgcctt ggtgcataac caatacacga tcactaatgc ctaaaacttc aggtaattcg
     2101 gaggaaatga cgataattgc cataccttct ttagctaatt ggttgatgag cttataaatt
     2161 tcatattttg ctcccacgtc gataccacgg gtcggctcat ctaaaatcag gattttaggg
     2221 ttaagcaata ggcattttgc caaaattgct ttttgctgat taccgccact taaccgtcca
     2281 attgctaatt caggcgagga agttttcact ttcagttttg caatagattg gttgataatg
     2341 gtttcttcca gcggttcatt tattactttt ttgccaaaac aaaattgcgg taatgaagag
     2401 agagttatgt ttttacccac ccccataatt ggaatgatgc cgtgtttttt gcgatcttcc
     2461 ggcaccatca caatatggtt ttcaatcgct tgtgcacaat ttttgatttt aatcggttgc
     2521 ttatgcagaa aaatatcacc ttgatatttg ccttggtaag agccaaaaat acattgtgcc
     2581 atctcggttc gccctgaacc gaccaaacca gcaataccta aaatttcacc ttggcgtagt
     2641 acaaaattag cattatcaac ccgtttgata tgtgtattgg ttgggtgcca agcggttaaa
     2701 ttttcaactc gaaggacttc ttcaccaata ttgtgtggct cgtgtgggta gagcgaggta
     2761 atctcacgtc caaccatcat cgtaataata tcgtcttcac tcattccctc tgcactgcga
     2821 gtaccgatat gttcgccgtc tcgaatgaca cagattttgt cggaaatggc tttcacttca
     2881 ttcaatttgt gtgaaatata aacacaagca atattgtgag cttgtaaatc tttaattaaa
     2941 ctcagcagaa tctcggtttc tttttcggtt aacgaggcgg taggctcgtc taaaatcaac
     3001 aaccgtacct gtttattcaa tgctttggca atttcaacca actgttgttg ccctaaacct
     3061 agctctccaa ctttggtgtt tgggtcaata tccaactgca cttgttcaag taaggtttta
     3121 caacgcagat acatttcatt gtcatctgta atccctgatt tggtaatttc attccctaaa
     3181 aacatattct ccaaaatcga catatttttt actaaggtca attcttggtg aataatcgaa
     3241 atgccttttt cttcagtatc tttgatattt ctggcaatca atttttcgcc ggaaaaatag
     3301 atttcccctt cataatcacc ggcaggataa attccacata atactttcat cagagttgat
     3361 ttgcctgaac cattttcacc acataaagaa agtatttcgc cggtttcaag ggaaattgaa
     3421 atattattta atgccacaac ctcaccgaat tttttagtga tgttcttcat ttctaataat
     3481 ttcgccatac ttagttagcc ccttattttc aaaaatggcg aggaagcctc gccatctaga
     3541 aaaattaaag tgatttattt atatactgca tctttggtat ggaagccgtc tttaattact
     3601 gtgtcatcaa tattttcttt agttacaaca accggctcta ataaatatgc cggtacttct
     3661 ttcataccgt tatttaattg agcattcatt tctactttct catctttacc taatgcaaca
     3721 gcaatttcgg cagctttgtc ggctaaattg gtaattggtt tatacactgt catcgtttgc
     3781 gtaccggcaa taatacgttt aattgcggct aaatctgcat cttgtccgga aattgcaacc
     3841 ttgctggata acccttgggc acttaacgct tgaattgcac cgcctgcggt tgcatcgttt
     3901 gaagctacaa ctgcatcaat attattttta ttagcagtta gtgcattttc cataatctgt
     3961 aatgcttttt cagcaagcca agaatctacc cattgatcgc ctaccacttt aatttttcca
     4021 ctgtcaatta acggttgtag caccttcatt tgacctttac ggaacagctt cgcgttatta
     4081 tctacaggtg aaccacccat taggaaataa ttcccctccg gcttttgccc aataatgctt
     4141 ttagcctgca attcacctac tttttcatta tcaaatgaaa cataaaaatc cacatcggca
     4201 ttattgatta agcggtcgta agcaagtacc ttaacacctt ctttcttcgc ctcggcaatc
     4261 acattggata acacttcgcc gttaaacgga ataatcacca atacatcaat atctttattc
     4321 aacatattct caatttgtga gatttgtgcg gttgcatcgc catttgccga ttgaacgaag
     4381 acttttgctc ctaagctttc cgctttattc acaaaaatat ctcggtcttt ttgccaacgc
     4441 tctaagcgta aatcatcaat tgacataccg atttttagat ctttagcaac agaagcattg
     4501 ctgagtacca ttaaagtcgc cgtagcaacg gctaataatt tagacttaag tttcatagtt
     4561 cacctcattg gttatgaaaa tgtgagcatc tttttcgccc gtaagcagt
//
LOCUS       DANLXF010000186         4593 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.186, whole genome shotgun sequence.
ACCESSION   DANLXF010000186 DANLXF010000000
VERSION     DANLXF010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.186"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(13..1860)
                     /gene="selB"
                     /locus_tag="PXD66_002039"
     CDS             complement(13..1860)
                     /gene="selB"
                     /locus_tag="PXD66_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HDL6320302.1"
                     /translation="MIICTAGHVDHGKTSLLQALTGTNTAHLPEEQKRGLTIDLGYAY
                     LPIENDILGFIDVPGHQRFLSNMLAGLGGIQHALLVISAEEGIKPQTEEHLDILHLLN
                     FQHIMIVITKADRAEETQISHLIEQVKTSYPFLAEAKTFVTSAKTQQGIEELKAHLIQ
                     LNQQHTQTHKPFRYAIDRVFNVKGAGLVVTGTTVAGKVSVGSELFLSNGKKVRVKAIH
                     AQNTPSETGVAGQRLALNIANVEKEEIKRGDWITELEPKFATDRITVQLTANQAFKEN
                     SIVHLYHFASHITGKLNLLEGQQAVKNQQFFAEVILDEPLHIAVGDKLIIRSGDDSLT
                     LAGAEVLEIHSPKRHKRTEARLALVKNLAKTTACNDFATRLDLYLQNRAMDLSLLLWA
                     EQCFAEDIAKLGFDVSSKWLFNSDFKAQAQQRVLEKMAEYHLQHQDQIGVTKARLYRI
                     ALLDLPENLANQFIEDLVEQKALVNSRGWLHLPEHRIELNADELQIWQQIRPLFEATN
                     QALWVRDIASQLSIDETQMRNLLYKVGKLGYLIPIVKDRFLLSEQIGQFATLIKDFIA
                     QNGSISVNQLRDELQYGRKLTVQLIEYFDRSGFLRRKGDVHLLRDSETF"
     gene            complement(1870..2166)
                     /locus_tag="PXD66_002040"
     CDS             complement(1870..2166)
                     /locus_tag="PXD66_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL6320303.1"
                     /translation="MMKFDYNIVWLQKAEIKLFYQADYILQQSQNPIIAEQFYDAIKS
                     EVDKLSFTADVYRLRKKKEIPILNGKYQVKFLIGQENVYIVDFKSARQNSYSFL"
     gene            complement(2163..2339)
                     /locus_tag="PXD66_002041"
     CDS             complement(2163..2339)
                     /locus_tag="PXD66_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320304.1"
                     /translation="MELILAQDLAKLRTDKPQSFYTNDELDLLHQLENGLIETKSHAQ
                     VMEDLRKKLGITEK"
     gene            complement(2408..3040)
                     /gene="pdxH"
                     /locus_tag="PXD66_002042"
     CDS             complement(2408..3040)
                     /gene="pdxH"
                     /locus_tag="PXD66_002042"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005575912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HDL6320305.1"
                     /translation="MDLHHIREDYTKKVLSIRDCHSNPITQFEQWLSEAINAKVNEPT
                     AMNIATVAANGRPSSRMVLLKEVNSQGFVFFTNYQSRKGWEIADNPFGCLTFFWAELQ
                     RQVRIEGKIAKIPEAESDAYFDSRPYTSRLGAWASEQSQPIENYKALLLKSAQFALKY
                     PLSVPRPPHWGGYILIPDSIELWQGRSSRLHDRIHYWLENNEWKKERLSP"
     gene            complement(3098..4480)
                     /locus_tag="PXD66_002043"
     CDS             complement(3098..4480)
                     /locus_tag="PXD66_002043"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HDL6320306.1"
                     /translation="MQQLFRQIPSLDLLLKKPQAVELCQKFAYSSVAEQARSLLTQAR
                     QFISQQQALPDFIADENLFFIRLEQKVQALSEVKIRSVFNLTGTVLHTNLGRGLWSEK
                     AIQAATNAMRNNVALEFDIEAGKRSHRDNYISELLQILTGAEAACIVNNNAAAVLLML
                     ATFAQGKEVIVSRGELVEIGGAFRIPDIMAQAGCKLVEVGTTNRTHLKDYRNAITENT
                     AFLMKVHTSNYHIEGFTSSVSEEELVALGKEFNLPVITDLGSGSLTDMQALNLPAEPK
                     VQQKVAAGVDLVSFSGDKLLGGPQAGIIVGSQAMISQLQQHPLKRVLRCDKVILSALE
                     ATLRHYLFPEQLTDNLPTLHLLTQPLETLQHKAERLKQALHKRLNSAYILQIELSLAQ
                     IGSGALPTETLPSVAVTISAEKQSDLIALEQQFKTYPSPIIGRFAQQKFWLDLRSVAE
                     FDKLIEMLEY"
BASE COUNT         1236 a         1039 c          925 g         1393 t
ORIGIN      
        1 taatgccaca agctaaaatg tttcactatc tcttaacaaa tgcacatcgc cttttcgtct
       61 taaaaaaccg ctacgatcaa aatattcaat taattgtacc gtcaattttc gcccatactg
      121 caactcatct cgcaactggt tcaccgaaat gctgccgttt tgggcgataa aatctttaat
      181 taacgttgca aattgcccga tttgttcgct taataaaaat ctatctttga cgattgggat
      241 taaatagccc aatttcccga ctttatacag caaattccgc atttgggttt catcaatcga
      301 gagctggctt gcaatatccc tcacccaaag agcttgattg gttgcctcaa acagcggtcg
      361 aatttgttgc caaatttgca attcatcggc gttcaattcg attcgatgtt ccggcaaatg
      421 taaccaacca cgactattga ccaaggcttt ttgctccacc aaatcctcaa taaattgatt
      481 tgccaagttt tcaggcaaat ccaataacgc tatgcgatat aaacgagctt tggtcacgcc
      541 gatttgatct tgatgttgca ggtggtattc tgccattttt tccaacaccc gttgctgggc
      601 ttgagcttta aaatccgaat taaataacca tttagacgag acatcaaacc ccagttttgc
      661 aatatcctct gcaaaacatt gctccgccca taacagcaag gataaatcca tcgctctgtt
      721 ttgcagatag agatcaaggc gtgtcgcaaa gtcattacaa gcggtggttt ttgctaaatt
      781 ttttaccaaa gctaaacggg cttcggtacg tttatgccgt tttggtgagt gaatttccag
      841 cacctccgct ccggctaacg ttaggctgtc atcaccgctg cggatgatca acttatcacc
      901 aacggcaata tgaagtggtt catctaaaat cacttctgca aaaaattgct gatttttgac
      961 cgcttgttgc ccctcaagca aattcaattt gccggtaatg tgcgaagcaa aatggtaaag
     1021 atgcacaatg ctgttttctt tgaaggcttg attggcagtg agttgaaccg taatgcgatc
     1081 ggtggcaaat ttcggctcaa gctcggtaat ccaatcgcct cgcttgattt cctctttttc
     1141 cacgttagcg atatttaacg ccaaacgttg ccctgccact cccgtttccg aaggcgtatt
     1201 ttgggcgtga atcgctttga cccgcacttt tttaccattc gacaaaaata actcagagcc
     1261 aacgctcact tttcccgcca ccgttgtgcc ggtaacgacc aaccctgctc ctttcacatt
     1321 aaacacacgg tcaatcgcat aacggaacgg tttatgggtt tgggtatgtt gttgatttaa
     1381 ttgaattaaa tgtgctttta attcttcaat cccttgttgc gtttttgccg aagtaacaaa
     1441 ggtttttgct tccgctaaaa acggatagct tgttttcact tgctcaatta agtggctgat
     1501 ttgagtttct tccgcccgat ctgccttagt aatcacaatc ataatatgct ggaaattcag
     1561 caggtggaga atatccaaat gctcttcggt ttgtggctta atcccttctt cggcagaaat
     1621 caccaacagg gcgtgttgaa tgccgccaag ccctgccagc atattagata aaaaacgctg
     1681 atgacccggc acatcaataa accctaaaat atcgttctca atcggtaaat aggcatagcc
     1741 caaatcaatg gttagcccac gtttttgttc ttcgggcaaa tgggcggtgt tagtgccggt
     1801 taaggcttgt aaaagcgatg ttttgccgtg gtcaacgtga ccggcggtgc agatgatcat
     1861 tgatagtcct tataaaaatg agtaactatt ctggcgagca gatttaaaat ctacaatata
     1921 gacattttcc tgaccaatca ggaactttac ttggtactta ccgtttaaaa tgggaatttc
     1981 ttttttcttg cgaagtcgat aaacatcggc agtaaaggaa agtttatcca cttcactttt
     2041 tattgcatca taaaattgtt ctgcaataat cgggttttgg gattgttgta atatataatc
     2101 tgcttgataa aacaatttta tttctgcttt ctgtagccaa acaatattgt aatcaaactt
     2161 catcattttt ccgtgattcc caattttttg cgtaaatctt ccatcacctg agcgtgggat
     2221 ttagtttcaa ttaagccatt ttctaattga tgtaataaat caagttcatc attagtataa
     2281 aaactttgtg gcttatctgt gcgtaatttt gctaaatctt gtgcgagtat gagttccata
     2341 tttccccctt acaaacttag attattcaat attgtaacct gataaaacta aagttcaagg
     2401 agtgtattta aggcgataac cgctcttttt tccactcatt attttccaac caataatgaa
     2461 tgcggtcgtg tagccggcta cttctgcctt gccagagttc aatactgtcg ggaatcagaa
     2521 tatagccacc ccaatgcggt gggcgaggca ctgaaagtgg atatttcaat gcaaattgag
     2581 ctgattttag caacaaagcc ttgtagtttt cgataggctg actctgctcg ctcgcccaag
     2641 cccccaaacg gctggtgtaa ggacgagagt caaaataggc atcggattct gcctctggaa
     2701 tttttgctat tttcccttca atccgcactt gacgttgtaa ttctgcccaa aagaaggtta
     2761 aacatccaaa aggattatcg gcaatttccc accctttacg gctttgataa ttggtaaaga
     2821 acacaaagcc ttgtgaattt acctctttca acagcaccat tcggctactt ggtcgcccgt
     2881 ttgctgcaac ggtagcaata ttcattgctg tcggctcatt cactttggca ttgattgcct
     2941 cactcaacca ttgttcaaat tgggtgattg ggtttgagtg acaatctcgg attgaaagca
     3001 cttttttggt gtaatcttct cgaatgtggt gcagatccat aaggattcct ttatcttatt
     3061 ctaaataatc ggacaggcac agtctgccct accaaaatta atattccaac atctcaatca
     3121 acttatcaaa ctccgcgacc gagcgtaaat ctaaccaaaa tttctgctga gcaaaacgcc
     3181 cgataatcgg gcttgggtag gttttaaatt gttgttccaa tgcaattaaa tcgctctgtt
     3241 tttcagcgga aatagtcact gccaccgaag gtaaggtttc tgtgggtaac gcaccgctgc
     3301 cgatttgagc gaggcttagc tcaatttgca aaatataagc agaatttaac cgcttgtgta
     3361 gtgcctgttt taatcgctct gctttgtgtt gcaaggtttc aagcggttga gtgagtaaat
     3421 gaagagtcgg caagttatcg gttaattgct ccggaaataa gtagtggcgt agcgttgcct
     3481 caagtgccga taaaatcact ttatcgcaac gtagcacccg ttttagcggg tgttgttgca
     3541 gttggctaat catcgcttgg ctaccgacaa taatgccggc ttgcggcccg cctaataatt
     3601 tatcgccaga gaaggaaacc aaatctaccc cagccgctac tttttgctga acctttggct
     3661 cagcaggcaa attaagagct tgcatatcag ttagcgaacc actgcctaag tcggtaatca
     3721 caggcaggtt aaactcttta ccgagagcca ccaattcctc ttccgacacc gaactggtga
     3781 acccctcaat gtggtagtta ctggtgtgaa ctttcattaa aaaggcggta ttttcggtga
     3841 tcgcgttgcg ataatccttt aaatgagtgc gattagtcgt accaacttcc accaatttgc
     3901 aaccggcttg agccataata tccggaatgc ggaatgcccc gccaatttcc accagctcgc
     3961 cacgggaaac gatgacttcc ttgccttgtg cgaaggttgc taacatcaac aacaccgctg
     4021 ccgcattatt attcacaatg caagcggcct ccgctccggt aagtatttgc aacaactcgg
     4081 aaatgtagtt atctcggtgc gagcgcttgc ctgcttcaat atcaaattcc aacgccacat
     4141 tgtttcgcat tgcgttggtt gctgcctgta tcgccttttc cgaccacaac ccacgcccta
     4201 agttggtgtg taacaccgta ccggttaagt tgaacacgga acggattttc acctcactca
     4261 acgcttgcac tttttgttcc agccttataa aaaacaggtt ttcatcggca ataaaatccg
     4321 gcaaggcttg ctgttgggaa ataaattgac gtgcttgagt aagcaaactc ctagcttgtt
     4381 cagccacgct ggaatatgca aatttttggc ataattcgac cgcttgtggc tttttcaaca
     4441 agaggtcgag cgagggaatt tggcgaaata gttgttgcat aatgtgaatg attgagagaa
     4501 tattttggac aattctaata aataaagtac aatattgcca atttttcgga agtgcgtcat
     4561 ctccggtgag gtggcaggac ttcaaatcct gtt
//
LOCUS       DANLXF010000187         4545 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.187, whole genome shotgun sequence.
ACCESSION   DANLXF010000187 DANLXF010000000
VERSION     DANLXF010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4545
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.187"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..599)
                     /locus_tag="PXD66_002044"
     CDS             complement(<1..599)
                     /locus_tag="PXD66_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595-like element ISMha4 family transposase"
                     /protein_id="HDL6320307.1"
                     /translation="MRKSRLSQYKQNKLIEQFVAGVTARTASELVNVNKNTAAYYFHR
                     LRLLIYQHSPHLEMFEGEIEVDESYFGGHRKGKRGRGAAGKTAVFGLLKRDGKVYTVV
                     VPNTQFATLLPIIREKVKPDSIVYTDFYRSYDVLDVSEFNHFRINHSTHFAENQNHIN
                     GIENFGSQAKRHLRKFNGIPKVHFELYLKECEWRFNHSD"
     gene            928..2286
                     /gene="mnmE"
                     /locus_tag="PXD66_002045"
     CDS             928..2286
                     /gene="mnmE"
                     /locus_tag="PXD66_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDL6320308.1"
                     /translation="MKDTIVAQATPIGRGGVGILRVSGPLAETVAQAVLGKTLPPRIA
                     NYLPFKDEDGTVLDQGIALFFKAPNSFTGEDVLELQGHGGQVILDLLLKRILQVKGLR
                     IARAGEFSEQAFLNDKLDLAQAEAIADLIDATSEQAARSALKSLQGEFSNKINALVDS
                     VIYLRTYVEAAIDFPDEEIDFLADGKIEAKLNEIIAQLDGVRREAKQGSILREGMKVV
                     IAGRPNAGKSSLLNALAGREAAIVTNIAGTTRDVLREHIHIDGMPLHIIDTAGLRDAS
                     DEVERIGIQRAWEEIAQADHVLLMIDSTEQQADQFRQEWAEFLAKLPANMPVTVIRNK
                     VDLSGEQEGLIQVDDFTMIRLSAQTKVGVDLLREHLKKSMGYQSSTEGGFLARRRHLQ
                     ALETAAEHLERGHIQLTQFLAGELLAEELRMVQNALSEITGQFTSDDLLGNIFSSFCI
                     GK"
     gene            2287..3552
                     /gene="tilS"
                     /locus_tag="PXD66_002046"
     CDS             2287..3552
                     /gene="tilS"
                     /locus_tag="PXD66_002046"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDL6320309.1"
                     /translation="MALFNRFQTQCATHLPNQTAFLIGLSGGVDSVVLLHLFARTSFH
                     VRAIYVHHGLSPNADDWAAFCEQYCKRLNIPFILQKVRVDSSNGVENGAREARYQAIQ
                     QQLKPNEVFATAHHLDDQAETFLLALKRGSGVKGLSAMQAVTFLQNFTVFRPLLTFTK
                     SDLMGYAVQHQLGWIEDESNADNRYDRNFLRNSILPLLNQRWQHFSQMVARSAQHCAE
                     QQALIEELLSNELKSRTGEKQQLNINGFGQFSLAKQQQLSRLWLEQNGVRMPSQAQLQ
                     AVISELIFAKADKNPQVKVGENIIRRYQQAIYVTEEIADIPPFEVVLPTETKVELPYQ
                     LGTIIRQQQEIICKKNGKNHRLQLPQELAQSTISIKIGQLGKVKCYGKAQREEMKKIW
                     QNHGIPVWERNRTPLIFWQDELVGLLPIL"
     gene            complement(3542..4435)
                     /gene="xerD"
                     /locus_tag="PXD66_002047"
     CDS             complement(3542..4435)
                     /gene="xerD"
                     /locus_tag="PXD66_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDL6320310.1"
                     /translation="MAELDPIIEQFLDTLWQEHGLSENTVASYRSDLERFSDYFSEPK
                     AFLSLDHIDLQGFLGERLEQGYKATSSARMLSCLRKFFRFLCLEHYRQDDPTLTLSSP
                     KRGASLPKSLSEDQVMDLLDAPNTLDPIELRDKAMLELLYATGLRVTELVSLSMDNIS
                     LKQGVVRIIGKGDKERLVPLGEEANYWIQEFFQYGRPILLNNQQSDVVFPSRRGQQMT
                     RQTFWHRIKHYAVLAGIDADKLSPHVLRHAFATHLVNHGADLRVVQMLLGHSDLSTTQ
                     IYTQVAKARLKSLHQQFHPRG"
BASE COUNT         1318 a         1056 c         1005 g         1166 t
ORIGIN      
        1 aagtcactgt gattaaaacg ccattcgcat tctttcaaat acagctcaaa atgcactttg
       61 ggaatgccgt taaacttacg caaatggcgt tttgcttggc tcccaaaatt ctcaattcca
      121 ttaatgtgat tttgattttc agcgaagtgt gtgctgtgat taatgcgaaa atggttaaat
      181 tcactcacat caagcacatc ataacttcga tagaaatcgg tgtaaacaat actatcgggt
      241 ttcacctttt cccgaataat cggcaaaagt gtggcaaatt gcgtgtttgg tactaccacg
      301 gtataaacct ttccgtcccg ctttaaaagc ccgaacacgg cggttttgcc tgctgcacca
      361 cgtccacgtt tacctttacg atgaccgccg aaataactct catcaacctc aatttctccc
      421 tcaaacattt ccaaatgagg gctatgttga tagataagca accgcaagcg atggaagtaa
      481 tacgccgccg tgtttttgtt tacattgacc aactcagatg ctgttcgggc tgttacccct
      541 gcaacaaact gctcaatgag tttgttttgt ttgtactgac ttagacgact ttttctcatt
      601 tgggtatctt aacctaaatg gagtttttag tcgttatcta ggacagcccc aaagaaaaac
      661 acgctttgca ttgaaaaaaa ggcggtggtg gaaataaaac ggcgtaggag taatagtttt
      721 tctaaagaca ttcagaatgg ttgaattggt taaataaaga gcggttattc taccttttaa
      781 gtcaacccca atcaatcggc ttttgtttta cctttggcgt gggtttcggt atgataagcg
      841 atatgtagtg gcgggtcctg tgcccgccta aataccaatg ataacttgcg ggcgagcaca
      901 ggactcgccc ctacgataaa caagaaaatg aaagacacca ttgttgctca agcaacaccg
      961 attggcagag gcggtgtggg gattctgcga gtttccggtc cgcttgccga aaccgttgct
     1021 caagccgtat taggcaaaac attgccaccc cgtatcgcaa attatttgcc gtttaaagac
     1081 gaagatggca ctgtgttgga tcaaggcatc gccttatttt ttaaagctcc caactccttt
     1141 accggcgaag atgtgcttga acttcaagga cacggtgggc aggttatttt agatttactg
     1201 ctcaaacgca ttttacaagt taaggggcta cgcattgccc gagcaggtga attttccgaa
     1261 caggcatttt taaacgataa gctcgacctt gcccaagctg aagcgattgc ggatttaatc
     1321 gacgccacca gcgagcaagc cgctcgttca gccctgaaat cactacaggg tgaattttct
     1381 aataaaatca atgcgttagt tgattcagta atttatttac gcacttatgt ggaagcggcg
     1441 attgacttcc ccgatgagga aattgacttt ctggcagacg gtaaaatcga ggcaaaatta
     1501 aatgagatta tcgctcaatt agacggtgtt cgccgtgaag cgaaacaagg ctcgattctg
     1561 cgtgaaggaa tgaaagtagt gatcgccggt cgcccgaatg cagggaaatc aagcttgctt
     1621 aacgccttag ccggacgaga ggcggcgatt gtaaccaata ttgctggcac aacccgtgat
     1681 gtgttgcgtg aacatattca cattgacgga atgccgttgc atattatcga caccgccggt
     1741 ctccgtgatg ccagtgatga ggtggaacgt atcggcattc agcgtgcttg ggaagaaatc
     1801 gcccaagccg accacgtttt attaatgatt gacagcaccg agcaacaagc cgatcagttc
     1861 cgacaagagt gggcggaatt tctagccaaa ctgccggcaa atatgcctgt gaccgtaatt
     1921 cgcaacaaag tcgatttatc cggcgagcag gaaggcttaa ttcaggtgga cgatttcacg
     1981 atgattcgcc tctctgccca aaccaaagtc ggcgttgatt tattgcgtga acacttgaag
     2041 aaatcaatgg gctaccaaag ttctacggaa ggcggattct tggctcgccg ccgtcatctg
     2101 caagcattgg aaaccgctgc cgagcattta gagcgagggc atatccagct tactcaattc
     2161 ttagccggcg aattgttagc ggaagagttg cgtatggtgc aaaatgcctt aagtgaaatt
     2221 accggccagt tcacctctga tgacttatta ggcaacattt tcagctcatt ttgcatcggt
     2281 aaataaatgg cactttttaa ccgctttcaa acccagtgtg caacgcattt gccgaaccaa
     2341 accgcctttt taatcggctt aagcggtggg gtggattcgg tggtactact ccaccttttt
     2401 gcccgcacct catttcacgt tagggcaatt tatgtccacc acgggctaag cccaaatgcg
     2461 gacgactggg cagccttttg cgaacaatat tgcaagcggt taaatattcc ctttatttta
     2521 caaaaggtca gagtggatag ctcaaacggc gttgaaaacg gggcgagaga agctcgttac
     2581 caagccattc aacaacagct taagcccaat gaagtgttcg ccaccgccca ccatttagat
     2641 gaccaagccg aaactttttt gctcgccctc aagcgaggca gtggagtcaa aggattatcg
     2701 gcgatgcaag cggtcacttt tttgcaaaat tttaccgttt ttcgaccgct actcactttt
     2761 actaagtcag atttgatggg ttatgccgtt caacatcagc taggttggat tgaggacgaa
     2821 agcaatgccg ataaccgtta cgaccgtaat ttccttcgca atagcatctt gccactactc
     2881 aatcaacgtt ggcagcactt cagccaaatg gttgcccgtt ccgctcagca ttgtgcagaa
     2941 cagcaagcct tgatagaaga gctattaagc aatgaactca agtcaagaac aggggaaaaa
     3001 caacaactca acattaacgg ctttgggcaa ttttcacttg ccaagcaaca gcaacttagc
     3061 cgcctttggc tggaacaaaa cggagtgaga atgccaagcc aagcccagct acaagcggtc
     3121 atttctgaac tgatttttgc aaaagccgat aaaaacccac aggttaaagt cggcgaaaat
     3181 attatcagac gctatcaaca agctatttac gttaccgaag agatcgccga cattccgccg
     3241 tttgaggtag tacttcccac agaaaccaaa gtggaattac cctatcagct cggtactatt
     3301 attcgccaac aacaagagat catttgcaaa aaaaacggga aaaatcaccg cttgcaactg
     3361 ccccaagagc tggctcaatc aactatttct attaaaatcg ggcaactagg caaagtaaaa
     3421 tgctacggca aggctcaacg ggaagaaatg aaaaagattt ggcaaaatca cggcatacct
     3481 gtttgggaac gcaaccgtac accgctgatt ttttggcaag acgaattagt tggcctatta
     3541 cctatcctct agggtgaaat tgctgatgaa gcgatttcaa acgggctttt gccacttgag
     3601 tgtaaatctg ggtggtcgat aaatcactat gccccagcaa catctgtacc actcgcaaat
     3661 cagccccatg attgaccaaa tgggtcgcaa aggcgtggcg aagcacgtga ggcgagagtt
     3721 tatctgcatc aatacctgca agcacggcat agtgcttaat ccgatgccaa aacgtctgcc
     3781 ttgtcatctg ctgacctcgc cggctcggaa atacaacatc agactgctga ttattcagca
     3841 aaatagggcg accgtactgg aaaaactcct gtatccaata attcgcctcc tcgcccaatg
     3901 gcactaaccg ctctttatcc cctttaccga taattctcac caccccttgt ttgaggctga
     3961 tattatccat cgaaagcgac accagctccg ttacccgcag accggtggca tacagcagct
     4021 ccagcattgc tttatcccgt aactctatcg gatctaacgt attcggggca tctagcaaat
     4081 ccattacctg atcttcactt aaggatttgg gtaaagaagc accccgttta ggggaagaaa
     4141 gggttaaagt cgggtcatct tggcgataat gctcaaggca taaaaaacgg aaaaatttcc
     4201 ttaaacaact caacattctg gcagagcttg tcgccttata gccttgctcc aaacgctcgc
     4261 cgagaaaacc ttgtaagtcg atatgatcga gagacagaaa tgctttaggt tcagaaaaat
     4321 aatcggaaaa acgctctaag tcagaacgat aagaggcaac ggtattttca gacaaaccgt
     4381 gttcttgcca tagtgtatcg agaaattgtt caataatagg gtcaagctca gccatacaag
     4441 cgggtatatt ttcaggaaat tttgcaaatt ttaacagaat taaatctata aaaatacaaa
     4501 agcgatagaa acgaaaaaac cgattagctt atgctaatcg gttta
//
LOCUS       DANLXF010000188         4471 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.188, whole genome shotgun sequence.
ACCESSION   DANLXF010000188 DANLXF010000000
VERSION     DANLXF010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.188"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            83..3979
                     /gene="purL"
                     /locus_tag="PXD66_002048"
                     /gene_synonym="purI"
     CDS             83..3979
                     /gene="purL"
                     /locus_tag="PXD66_002048"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HDL6320311.1"
                     /translation="MTIQTFRGSPALSAFRIQQLLQKFQQNQLPVKSVYAEFLHFVAL
                     NRPLVADEENKIKELLHYGPTLEEDEPTGFCLIVTPRIGTISSWSSKATDIAHNCGLA
                     AVERIERGTAYYLEFSETPNESQLTTLKGLLHDRMMETVLDNESQAEQLFAQQEPKPF
                     TSVDILGGGRKALEEANINLGLALADDEIDYLVENFTQLGRNPNDIELYMFAQANSEH
                     CRHKIFNADWTIDGVKQEKSLFKMIKNTFEKTPDYVLSAYKDNAAVMEGSTVGRFFPD
                     QDGQYRYHQEDAHILMKVETHNHPTAISPFPGAATGSGGEIRDEGATGRGAKPKAGLT
                     GFSVSNLVIPKFEQPWENPLSKPNRIASALDIMIDGPLGGAAFNNEFGRPALLGYFRT
                     YEEKVNSFAGEEIRGYHKPIMLAGGIGNIRAEHVQKGEIPVGAKLIVLGGPAMNIGLG
                     GGAASSMASGKSKEDLDFASVQRENPEMERRCQEVIDRCWQMGAENPILFIHDVGAGG
                     LSNAMPELVHDGECGGKFDLRNILCDEKGMSPLEIWCNESQERYVLAVSPEKLDIFTA
                     LCERERAPFAVIGEATKEKHLTLADPHFDNKPIDLPMNVLLGKTPKMQRDVLSKTVKN
                     PPLATADIDLKEAFHRVLRLPAVAEKTFLITIGDRSVTGMVAQDQMVGPWQIPVADCA
                     VTTASLDSYHGEAMSIGERSPVALLDFAASARLAVAESITNIAATNIGDIKRIKLSAN
                     WMSAAGHEGEDAGLYEAVKAVGEELCPELGITIPVGKDSMSMKTTWEENGEQKSVTAP
                     LSLVISAFARVEDVRKTVTPQLRTDKGDTRLLLIDLGEGNNRLGATALAQVYKQLGDK
                     PADVVNVARLKGFFNAMQTLVAQGKLLAYHDRSDGGLITTLAEMAFAGNCGISVEIDR
                     LVDDLAVLFNEELGAVIQVRTEELSEVQAVLNAENLANLTFDIGTAVESDSFEITRNG
                     TTVFSQKRSELRGIWAELTHQMQRLRDNPECADQEFAAKKDPNNKGLSAFLTYDVNED
                     IAAPYINKGVKPSVAILREQGVNSHYEMAAVFDRAGFNAIDVHMSDLISGRRNLNDFN
                     AMVACGGFSYGDVLGAGGGWAKSILFNPMLRDQFSKFFANPNTLALGVCNGCQMVSNL
                     AEIIPGTENWPRFVRNKSERFEARAAMVKINDTNSLWFNGMAGSHMPIAVSHGEGQVE
                     FKNPQQLANLQQQNLVIAQYVDSHLNPTEQYPANPNGSALGITAIGNTDGRVAIMMPH
                     PERVFRAVSNSWCPEDWTEGGAWMRLFRNARVALKY"
     gene            4334..4471
                     /locus_tag="PXD66_002049"
                     /pseudo
     CDS             4334..4471
                     /locus_tag="PXD66_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT         1245 a         1023 c         1112 g         1091 t
ORIGIN      
        1 aacgtctgac aaatatagca atcgtttgcg caatcgttta taatcggttt agtttttcat
       61 cttaaatagg aaaatccaaa gaatgacaat ccaaaccttc cgtggctcgc ctgccttatc
      121 tgcttttcgc attcaacaac ttctgcaaaa attccaacaa aatcaattac ctgtaaaatc
      181 agtttatgcg gagtttttgc attttgtggc attaaatcga ccgcttgtag ccgatgaaga
      241 aaacaaaatc aaagagttat tgcactacgg tccaacgctg gaagaagacg agccgacagg
      301 gttttgcctg attgttaccc ctcgtatcgg cacgatttcc tcgtggtcgt ctaaggcaac
      361 cgacatcgcc cataactgcg gtttggcagc agtagagcga attgagcgtg gcacggcata
      421 ttatttagaa ttttctgaaa cgccgaacga aagtcaactt acgacgttga aaggcttatt
      481 acacgaccgt atgatggaaa cggtgctgga taacgaaagc caagccgaac agcttttcgc
      541 tcagcaagag ccgaagccgt ttacttcggt ggatatttta ggcggtggtc gcaaggcact
      601 ggaagaagcg aatatcaact taggtttagc gttggcggac gatgaaattg attacttggt
      661 ggaaaacttt acccaacttg ggcgtaaccc caatgatatt gaactctata tgttcgccca
      721 agccaactcg gagcattgcc gccacaaaat ttttaatgcg gattggacga ttgatggggt
      781 aaaacaggaa aaatccctgt ttaagatgat taaaaacacc tttgagaaaa ccccggacta
      841 tgtgctttcc gcctacaaag acaatgcggc ggtgatggag ggctcaacgg ttgggcgttt
      901 cttccccgac caagatggac aatatcgcta tcatcaagaa gatgctcata ttttaatgaa
      961 agtagaaact cacaaccacc caaccgccat ctcgccattc ccgggagcgg cgaccggttc
     1021 gggcggtgaa attcgtgatg aaggggcgac agggcgtggg gcgaaaccga aagcgggctt
     1081 gacgggtttt tcggtctcta acttagtgat cccaaaattt gaacagccgt gggaaaaccc
     1141 gctttccaag ccgaatcgca ttgcctccgc actggatatt atgattgatg gcccactagg
     1201 cggagcggca tttaacaatg aatttggtcg tcctgcgtta ttgggctact tccgtactta
     1261 cgaagaaaaa gtgaacagct tcgcaggcga agaaattcgt ggctatcaca agccgattat
     1321 gttagcaggc ggtatcggca acattcgtgc cgaacacgtt caaaaaggcg aaatcccggt
     1381 gggagcgaag ttgatcgtgc ttggtggccc ggcaatgaac atcggcttag gcggtggggc
     1441 ggcatcgtca atggcatcgg ggaaatcgaa agaagatttg gattttgcct cggttcagcg
     1501 ggaaaaccct gaaatggaac gccgttgcca agaggtgatc gaccgttgct ggcagatggg
     1561 ggcggagaac ccaattttat ttattcacga cgtgggggct ggcggtttat caaatgcgat
     1621 gcctgaattg gttcacgacg gcgaatgtgg cggtaagttt gatttgcgta acatcttgtg
     1681 cgatgaaaaa ggaatgtcgc cacttgaaat ttggtgtaac gaatcgcaag agcgttatgt
     1741 gcttgccgtt tcccctgaaa aactcgacat tttcacagca ttgtgcgaaa gggaaagagc
     1801 cccatttgcg gtgattggcg aggcaacgaa agagaagcat ttaacccttg ctgaccctca
     1861 ttttgacaat aaaccgattg atttgccgat gaatgtgttg ctcggcaaaa cgccaaaaat
     1921 gcagcgagat gttttgtcaa aaactgtgaa aaatccaccg cttgcaaccg ctgacatcga
     1981 cttgaaagag gcgttccacc gtgtgctacg cttgcctgcg gtggctgaaa aaaccttcct
     2041 gattaccatc ggcgatcgct cagtaacggg aatggtggca caagatcaga tggtcggccc
     2101 gtggcaaatt cctgtggcag attgtgccgt aaccaccgct tcattagaca gctatcacgg
     2161 tgaagcaatg tcaattggcg agcgatcacc tgttgctttg cttgactttg ctgcctcagc
     2221 tcgcctcgcc gtggcagaaa gcatcaccaa tattgccgcc actaacattg gcgacatcaa
     2281 acgcattaaa ctctcggcga attggatgtc ggcggcaggg cacgagggcg aagacgcagg
     2341 gctttacgag gcagtaaaag cggtgggcga agagctttgc cctgaattag gcataaccat
     2401 tccggtaggc aaagactcaa tgtcgatgaa aaccacttgg gaggaaaacg gcgaacagaa
     2461 atctgttact gctccgcttt cgctcgtgat ttccgctttc gctcgtgtgg aagatgtgcg
     2521 taaaaccgtt accccacaac ttcgcaccga caaaggcgat acccgcttgt tgctgatcga
     2581 cctgggcgaa ggcaacaacc gcttgggtgc aaccgcactt gctcaagttt acaaacaact
     2641 tggcgacaaa cctgccgatg tggtgaatgt ggctcgctta aaaggcttct tcaacgcaat
     2701 gcaaaccctt gtcgcacaag gaaaattgct tgcctaccac gaccgctcgg acggtggttt
     2761 aatcaccaca ctggcggaaa tggcgtttgc agggaattgc ggaatttcgg tggaaatcga
     2821 ccgcttggtg gacgatttag ccgtgttatt caacgaagaa ctgggggcag taatccaagt
     2881 tcgcaccgaa gaattgagcg aggtgcaagc ggtgttaaat gccgaaaatc ttgcaaatct
     2941 cacctttgat attggtacag cggttgaaag cgacagcttt gaaattaccc gcaacggcac
     3001 aacagtattt agccaaaaac gttccgaact gcgtggcatt tgggcggaac tcactcatca
     3061 aatgcagcgt ttgcgagaca accctgaatg tgcagaccaa gaatttgcag cgaaaaaaga
     3121 cccgaataac aaaggcttat ccgcattttt aacttacgat gtgaacgaag acatcgccgc
     3181 cccatacatc aacaaaggcg taaaaccgag tgtggcgatt ttgcgtgagc aaggtgtaaa
     3241 cagccactac gaaatggcag ccgtctttga ccgtgccggt ttcaacgcca ttgacgtgca
     3301 tatgtcagac ttgatttcag gcagacgcaa cctcaacgac ttcaacgcaa tggtggcgtg
     3361 cggaggcttc tcttacggcg atgtcttagg tgcaggcggc ggctgggcga aatcgattct
     3421 gttcaaccca atgctgcgtg accaattcag caaattcttc gccaacccga acacgctggc
     3481 attaggggta tgtaatggtt gccaaatggt ctccaatcta gcggaaatta ttccaggcac
     3541 agaaaactgg ccacgcttcg tgcgtaataa atccgaacgt tttgaagccc gtgctgcaat
     3601 ggtgaaaatc aacgacacca actcactttg gttcaacgga atggcaggca gccatatgcc
     3661 gattgcggtt tcacacggtg aaggacaagt tgagttcaaa aatccacagc aattagcgaa
     3721 cttgcaacag caaaatctcg tgatcgcaca atatgtggat agccacctca acccaaccga
     3781 acaataccca gccaacccga acggatcggc gttgggtatc accgctatcg gtaatacaga
     3841 cggacgagtc gctattatga tgccacaccc tgaacgtgtg ttccgtgcgg tcagtaactc
     3901 ttggtgccca gaagattgga cggaaggtgg agcgtggatg cggttgttta gaaatgcgag
     3961 agtggcgttg aagtattaaa aatctacctt cgtagtgatc tgcccccata gggggcgatt
     4021 aaaattcgaa ttcatagaac ccgaattttt atatatctga taaacgaagt tgtttatcct
     4081 tcgtgctttt cctgatattt caagtacttt cttatcattt ctgcattgac atctacggtg
     4141 tctacgcagt agcctcttgc cacaaaagtg ggttgcccca caatttatgc ttacgtaaat
     4201 agggaaactt attgaaaaat cttattggac cctgttcacc cttttaaatg ccccattacg
     4261 tcttatacag aaaatgaacg tgatttattt gaacggtcaa ttctacctta atagaaagtg
     4321 gtttttagtt agcaatcaaa atcacattaa cggaattgag aatttggaga gtcaggcaaa
     4381 atgctatttg cgtaaattta acggcgttcc taaagtacat gaattctatt tgaaagaatg
     4441 cagatggcgt tttaatcaca gtaacttaaa a
//
LOCUS       DANLXF010000189         4452 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.189, whole genome shotgun sequence.
ACCESSION   DANLXF010000189 DANLXF010000000
VERSION     DANLXF010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4452
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.189"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            3..320
                     /gene="trxA"
                     /locus_tag="PXD66_002050"
     CDS             3..320
                     /gene="trxA"
                     /locus_tag="PXD66_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012073573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDL6320312.1"
                     /translation="MHTTDATFEQDVLKSDVPVLLDFWAPWCGPCRMIGPVLDDLSVD
                     AQFDGKVKIVKMNVDENPSVPAQFGVRSIPFLLLFKNGEVIAQQVGAIPHAQMAEFIK
                     QAL"
     gene            491..1714
                     /gene="hflK"
                     /locus_tag="PXD66_002051"
     CDS             491..1714
                     /gene="hflK"
                     /locus_tag="PXD66_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HDL6320313.1"
                     /translation="MSWNESGNQQDPWGKPGQKKAEPKPEQQEQGSQQEPTNNQRNEQ
                     QPPDLEEVFSSLLKKMGGGKGPNNSSNQPSASLGKFLPVVLGLAAVVWVGSGFYTVQE
                     AERGVVTRLGKLNDIVLPGLNWKPTFIDSVTRVNVERVSELNTSGSMLTQDENMVQVE
                     MTVQYRVEDPAKYLFSVSNPDDSLKQATDSALRYVIGHMTMDEILTTGRATVRERTWS
                     TLRDIIKTYDMGLLVTDVNFQYARPPEEVKAAFDDAIKAQEDEQRLIREAEAYARGEE
                     PIARGQAQRTIEQAQAYKEAVVLNAKGEVERLSQLLPEYKASPELTRERLYIQTMEKV
                     MKNTPKVVMDSSGNNLNVLPFDKLMNSSSVIKAEQPAAAQVVTPQVVQPQVQPRTQIQ
                     PQQAEQQEPTRKGRF"
     gene            1717..2604
                     /gene="hflC"
                     /locus_tag="PXD66_002052"
     CDS             1717..2604
                     /gene="hflC"
                     /locus_tag="PXD66_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HDL6320314.1"
                     /translation="MRRLLVPILAVVAFVVLQAITIVNEGERGIMLRFNKVHRDSDQK
                     VVVYEPGIHFKVPFIDSLKVLDARIQTLDGQEDRFVTVEKKDLLVDSYVKWRISDFGQ
                     FYTSTGGDYQKAADLLRRKVGDRLRSEIGSRTIKDIVSGSRGELMAGAQKALNAGEDG
                     AERLGIEVVDVRVKQINLPNEVSSSIYQRMRAERDAVAREHRSQGNEKAEVIRAEVDK
                     KVVLILANANKTAQALRGEGDAQAAKLYSEKFGNEPEFYSFIRSLKAYEDSFAEGQNN
                     MMLLKPNSEFLRFMQAPTK"
     gene            2778..4076
                     /gene="purA"
                     /locus_tag="PXD66_002053"
     CDS             2778..4076
                     /gene="purA"
                     /locus_tag="PXD66_002053"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010869263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDL6320315.1"
                     /translation="MGKSVAILGAQWGDEGKGKIVDLLTDRVKYVVRYQGGHNAGHTL
                     IINGEKTVLRLIPSGILRDNVTCLIGNGVVLSPEALMKEMGELEERGINVRERLKISE
                     ACPLILPYHVAMDHAREAALGKNKIGTTGRGIGPAYEDKVARRGLRVSDLFDKEHFAA
                     KLKDILDYYNFQLVNYYKVEAVDYQKTLDDVFAIADVITGMVADVTTLLHEARENGDN
                     ILFEGAQGTMLDIDHGTYPFVTSSNTTAGGVATGSGYGPRHLDYVLGIIKAYCTRVGS
                     GPFTTELFDDVGAEIARKGNEFGAVTGRPRRCGWFDAVAVRRAVQINSISGFCMTKLD
                     VLDGFKELKICVAYKMPNGKIVEYAPLAAKDWEGVEPIYETMPGWSENTFRVTKREDL
                     PQAALDYIKRIEELVGVPVDILSTGPDRVETMILRDPFAA"
     gene            complement(4236..4306)
                     /locus_tag="PXD66_002054"
     tRNA            complement(4236..4306)
                     /locus_tag="PXD66_002054"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4276..4278),aa:Arg,seq:tct)
BASE COUNT         1380 a          793 c         1037 g         1242 t
ORIGIN      
        1 tcattcatac aacagatgct acatttgaac aagatgtttt aaaatcagat gtaccggtat
       61 tattagactt ctgggcgcct tggtgtggtc cttgccgtat gatcggcccc gtacttgacg
      121 atttatctgt tgatgcacaa tttgatggta aagtaaaaat tgtcaaaatg aacgtagatg
      181 aaaatccatc tgtaccagca caatttggcg tacgcagcat tccattttta ctattattca
      241 aaaatggtga agtgattgcc cagcaagttg gtgcaattcc gcacgcccaa atggctgaat
      301 ttatcaaaca agcattataa ttttaagagc aatactaggg cgaatttatc gccctatttt
      361 tttgcaaata aatagtgaaa aaagaccgct tgtttcagat aaacctagtc aaaagcttac
      421 taaataggta caatcactaa cttgcattct aagctttaaa gtgcgattcg attttatttt
      481 ggagatattt atgtcgtgga atgaatcagg caatcagcaa gatccttggg gtaaaccggg
      541 gcagaaaaaa gctgagccaa agcctgagca acaagaacag ggttcacaac aagagccgac
      601 caataatcaa agaaatgaac aacagccccc tgatttagaa gaagtattta gcagtttatt
      661 aaaaaaaatg gggggtggaa aagggcctaa taatagttca aatcaaccta gtgcaagctt
      721 agggaaattt ctacctgtgg ttttaggtct tgcagcagta gtttgggttg gctcaggttt
      781 ttacactgtg caagaggctg aacgtggtgt ggttactcgt ttaggtaagc tgaatgatat
      841 cgtgttgcca gggttgaatt ggaaaccgac ttttattgat tcggttacaa gagtaaacgt
      901 agaacgtgtt tcagagctga acaccagtgg ctcaatgctg actcaagatg aaaatatggt
      961 gcaggtagaa atgacggttc agtatcgtgt ggaagacccg gctaaatatc tgtttagtgt
     1021 gagcaaccct gatgacagct taaaacaagc aacagacagt gccttgcgtt atgtgattgg
     1081 tcatatgacg atggatgaaa ttttaaccac agggcgtgca accgtgcgtg aaagaacgtg
     1141 gtcgacattg cgtgatatta tcaaaaccta tgatatgggt ttattggtaa cagacgtaaa
     1201 cttccaatat gcacgaccgc ctgaagaggt gaaagccgcc tttgatgatg caatcaaagc
     1261 acaagaagat gagcaacgct taattcgtga agcggaagcc tatgcaagag gtgaagagcc
     1321 gattgcacgt ggccaggcac agcgtacgat tgagcaagct caagcttaca aagaagcagt
     1381 tgtgttaaat gcgaaagggg aagtggagcg tttgagtcaa ttattacctg aatataaagc
     1441 ctctccagag ctgactcgtg aacgtttata tatccagaca atggaaaaag tcatgaaaaa
     1501 tacgccgaaa gtggtgatgg attcaagtgg taataatttg aatgtattgc ctttcgataa
     1561 gctaatgaat tcttcatcgg taataaaagc ggagcagcct gcggcggcac aagtagttac
     1621 accacaagtt gttcagccgc aagtacagcc aagaacacaa attcagccgc aacaagcaga
     1681 acaacaagaa ccaactcgta aggggagatt ttaatcatgc gtagattatt agtacctata
     1741 cttgctgtag ttgcttttgt tgtgctacag gcaatcacca ttgtaaacga gggcgagcgt
     1801 ggcattatgt tacgttttaa caaagttcat cgtgatagcg atcaaaaagt ggtggtatat
     1861 gaaccgggga ttcactttaa agtaccattt atcgatagtt taaaggtatt agatgctcgt
     1921 attcaaactc tagatgggca agaagaccgt tttgtaacag tcgagaaaaa agatttatta
     1981 gtggactcct atgtaaaatg gcgaatcagt gattttggcc aattttatac ctctactggg
     2041 ggggattacc aaaaagcggc ggatttatta cgccgtaagg taggggatcg tctgcgttcc
     2101 gagattggtt ctcgaaccat taaagatatt gtatccggct ctcgtggtga gttaatggca
     2161 ggagcccaaa aagcgctgaa tgccggtgaa gatggagcag agcgtttagg aattgaagtg
     2221 gttgatgtgc gagtgaagca aatcaactta ccaaatgaag tttcttcttc aatttaccaa
     2281 cgtatgcgtg ccgagcgtga tgcggttgct cgtgaacacc gctcacaagg taatgagaaa
     2341 gcagaggtaa ttcgtgccga agtggataaa aaagtggtgc tgattttggc aaatgcgaac
     2401 aaaactgccc aagccttgcg aggtgaaggt gacgctcaag ctgcaaaact ctattcagag
     2461 aaatttggta acgagccgga attttacagt tttattcgta gtttaaaagc ctatgaagat
     2521 agctttgccg aaggtcaaaa taatatgatg ttattaaagc caaacagcga attcttacgc
     2581 tttatgcaag caccgacaaa ataattgtat ctataaaacc cgatcttgta tcgggttttt
     2641 aattttcatc cctattctga tcataacaag cggtaaattt tggctgattt tttgcaaaaa
     2701 atttgaaaaa attgaccgct tgcgatatga agttataatg ggtttcgtta ttcatcctaa
     2761 ctaaaagagg catttctatg ggtaaaagtg ttgctattct tggcgctcag tggggcgatg
     2821 agggaaaagg caaaattgtt gatttattaa cagatcgtgt gaaatatgta gtacgttatc
     2881 aaggtggaca taatgcaggg cataccttaa tcattaatgg tgaaaaaacc gttctgcgtt
     2941 taattccatc gggtattttg cgtgataacg tgacttgttt aatcggtaat ggtgtagtgc
     3001 tttctccaga ggcattaatg aaagagatgg gggagctaga agaacgaggc attaatgtgc
     3061 gtgagcgttt aaaaatttct gaagcttgcc cgttaattct gccttatcac gttgcgatgg
     3121 atcacgctcg agaagcggca cttggtaaaa acaaaattgg tacaacagga cgtggtattg
     3181 gcccagctta tgaagataaa gtcgctcgcc gtggtttacg tgtcagcgat ttattcgaca
     3241 aagaacattt tgccgcaaaa ttaaaagata ttttagatta ctacaacttc caattagtca
     3301 attattacaa agttgaagcg gtagattatc aaaaaacctt agatgatgtg tttgcgattg
     3361 cggatgtgat taccggaatg gttgccgatg tgacaacttt acttcacgaa gcacgtgaaa
     3421 acggcgacaa tattttattt gaaggggctc aaggcacgat gttagatatc gaccacggta
     3481 cttacccgtt cgtaaccagc tcgaatacga cagcaggcgg tgtggcaacc ggatcaggct
     3541 acggtcctcg tcatttagat tatgtattag ggattatcaa agcctactgt acccgtgtag
     3601 gtagtggacc gttcaccacc gaactatttg acgatgtagg tgctgaaatt gctcgcaaag
     3661 gtaatgagtt cggagcggta accggtcgcc ctcgccgttg tggctggttt gatgccgtag
     3721 ccgttcgccg agcagtgcaa atcaactcca tttcaggctt ctgtatgact aaactcgatg
     3781 ttttagacgg attcaaagag ctaaaaatct gcgttgccta caaaatgcca aacggaaaaa
     3841 ttgtcgaata tgcccctctt gcagcaaaag actgggaagg tgttgaacca atttatgaaa
     3901 caatgccggg ttggtctgaa aatactttcc gtgtgacaaa acgtgaagat ttaccacaag
     3961 ccgcattaga ttacattaaa cgtatcgaag agctggtcgg tgtgccggta gatattcttt
     4021 ctacaggtcc ggatcgtgtc gaaacgatga ttttgcgtga tccttttgca gcttaataaa
     4081 agataaaata aagccattta gtgagataga gctagatggc ttttttgttt gaaccagtaa
     4141 actttacttt ggcacaattt tgtcacaata cagaaaatag ttctttagcg tgagtttttg
     4201 aggctgtgta agtatttgaa tttaaaagaa tttaatggca cgccctaaag gattcgaacc
     4261 tttgacccac gccttagaag ggcgataggt attttataaa aggcgtttat tttaactaat
     4321 aaaaacaaag acttatagag ataacccctc tatattacct accattattt cacatctttt
     4381 tacattaaat aacaccattt acggtacatt tatggaacac gttggtgatg agctagtctt
     4441 caaaagttac ta
//
LOCUS       DANLXF010000190         4437 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.190, whole genome shotgun sequence.
ACCESSION   DANLXF010000190 DANLXF010000000
VERSION     DANLXF010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4437
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.190"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            63..494
                     /locus_tag="PXD66_002055"
     CDS             63..494
                     /locus_tag="PXD66_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL6320316.1"
                     /translation="MSNQIALLLMSHGSFARAALASAELIVGQQENVAAIGVVAVDNV
                     ADIKRQMEQTIAELNTDKGLIILTDIVGGTPMNLASSQLTHPNVFACSGLNLPLLLEV
                     LMSRDGNLQEIRENLPLAYQNGFSLRTQQDFVSQEDDDDLL"
     gene            481..948
                     /locus_tag="PXD66_002056"
     CDS             481..948
                     /locus_tag="PXD66_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL6320317.1"
                     /translation="MICFSRIDDRLIHGQVVTTWVNLHRIEQIIVLNDAVAADPTQKQ
                     ILTMAAPQGIKVVAFGIEKFGEVLQKTQITRRTMLLFTNSIDVLRTVELGVSIDKLNV
                     GGMRYQARREQLSKAVSVTPEERQAFKTLLEKGVEVTVQMVPNDEKLNLQEII"
     gene            951..1718
                     /locus_tag="PXD66_002057"
     CDS             951..1718
                     /locus_tag="PXD66_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL6320318.1"
                     /translation="MLTALLVACWAGICALDDIGTQMIRRPLLIAPVVGLIMGDLETA
                     LIIGATLEVMWMGIGNVGAYSAPDMISGTAIGTALGIAAGEASTAVALAVPTSLLAQQ
                     LLILYRSGVCALNPIAERWAASGDFDKIFRIHYIPMLIAFSIRAIPTFLAVYYGADII
                     AQAVSALPAKVITGLNVAGKIIPAIGIGLLMLMMIKKAELWTFLIAGFTLAVYLKLSV
                     LPITLIALPLALIYDLAVSKKAEAAPTKTADEEDYDL"
     gene            1715..2521
                     /locus_tag="PXD66_002058"
     CDS             1715..2521
                     /locus_tag="PXD66_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL6320319.1"
                     /translation="MSNKITKQDLNKVFWRMQVLNVTNNFQSMQAIGFLSSFIPVLKR
                     LYKDRPQEERAEAMKRHLKFFNSHVNSDALILGIAAAVEETTKEDEKETVTAIKTGLM
                     GPLAGLGDSLLKFTWLPICGSIGAALALSGNVLGPILMFLLYNSVNIATKYYGIHLGY
                     SKGVDLIHGAGNNILQRLGNMANVVGLMVIGALIASVVKVKVVSQIAAGDNVIKFQEM
                     LDKVMPGLLSLLLTVVIYWILKKTNGKYAATMILATMGICIGLVYAGILG"
     gene            2521..3558
                     /locus_tag="PXD66_002059"
     CDS             2521..3558
                     /locus_tag="PXD66_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HDL6320320.1"
                     /translation="MDSVYDIIRLEQGRYLFRQQPAQSVQIFFLDNPDSKDSKWLAET
                     DLAEFELKLSACEARPFFLIQSANGEQIVAERTLPVAGMNNFRDMGGYVAHQGKRVKW
                     GKLYRSDHLHNLRDEGVAYLDKLGIQTVIDYRSPNEVAKYPNPPINGREQTFRLDPNA
                     HTAELAAQFSADKHDEDRNLVNKIIAQKAEGNLINRYDIVMAQYRNFVEKAECQTAFA
                     EMLRLATDPENAPLVQHCRGGKDRTGFGAMLLLGILGVSKADIIADYMLTHYNRLARN
                     EEKMAIYRTFTQDQDVLDYLLSLIDTQPEFIEQSLNTIETQYGTIEQYAQRVLGITAK
                     EIEALRANYLA"
     gene            3591..4437
                     /locus_tag="PXD66_002060"
                     /pseudo
     CDS             3591..4437
                     /locus_tag="PXD66_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251836.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine-type phosphatase"
BASE COUNT         1296 a          849 c         1031 g         1261 t
ORIGIN      
        1 tcccttgttt atctgtataa cattttattt aatagaatga cttataaatc aaggagtgac
       61 ttatgtctaa tcaaattgcg ttattgttga tgagccacgg cagttttgcg agggcggcgt
      121 tggcgagtgc cgagctgatt gtcgggcagc aggaaaatgt ggcagctatc ggcgtagtag
      181 cagtggataa tgtagcggac attaaacggc agatggagca aaccattgct gagttaaaca
      241 ccgacaaagg cttgattatt ctcaccgata ttgttggtgg tacgccgatg aatcttgcct
      301 cttctcaatt aacccaccct aatgtttttg cctgttcggg tttgaattta cctttattgc
      361 tagaagtgct gatgagccgt gacggcaatt tgcaagaaat tcgggaaaat ctaccgcttg
      421 cataccaaaa cggatttagc ctacgcacac aacaagattt tgtttcacag gaggacgatg
      481 atgatctgct ttagccgtat tgatgaccgt ttaattcacg gtcaagtagt caccacttgg
      541 gttaatttac accgtattga acaaattatt gtgttaaatg atgcggtggc agcggatcca
      601 acccaaaaac agattttaac gatggcagca ccgcagggaa ttaaagtggt cgcgtttggc
      661 attgaaaaat ttggtgaagt gttgcagaag acacaaatta cccgccgtac tatgttgttg
      721 tttaccaata gcattgatgt gttacgcact gtagaactag gtgtatcgat tgacaaattg
      781 aatgtcggcg gaatgcgtta tcaagctagg cgtgagcagt taagtaaagc ggtttcagtt
      841 acgccagaag agcgtcaagc atttaaaacc ttattggaaa agggtgttga agtaacggta
      901 cagatggtac cgaatgatga aaaacttaac ttacaggaga ttatctagct atgctgactg
      961 cactacttgt tgcctgctgg gcaggtattt gtgccttaga tgatattggc acacagatga
     1021 tccgccgtcc gttgttaatc gcaccggtgg tcggattgat tatgggggat ttggaaacgg
     1081 cgttgattat tggtgctacg cttgaagtga tgtggatggg gatcggcaat gttggtgcat
     1141 attctgcacc ggatatgatt tccggaaccg ctattggcac cgcacttggt attgctgctg
     1201 gtgaagcttc tactgcggtt gcactcgcag taccgacctc actcttggca cagcagttac
     1261 tcattcttta ccgctccggc gtatgtgcat tgaacccaat tgctgaacgc tgggcggcaa
     1321 gcggtgattt tgacaagatt ttccgcattc actatatccc aatgttaatt gccttttcta
     1381 ttcgtgctat tccaacgttc ttagccgtct attacggggc ggacattatt gcccaagcgg
     1441 tctctgctct gccggcaaaa gtgattaccg gattaaatgt tgcaggcaaa attattcctg
     1501 ccatcggtat cggtttgctg atgttaatga tgattaagaa agccgaactt tggacgttct
     1561 taattgctgg ctttacgttg gcggtttatc tcaaattatc agtattgccg attaccttaa
     1621 ttgcactacc gcttgcgttg atttacgact tagcagtaag caaaaaagct gaagctgcac
     1681 caaccaaaac cgcagatgaa gaggattatg acctatgagc aataaaatta ccaaacaaga
     1741 tttaaacaaa gtgttttggc ggatgcaggt gctgaacgtc acaaacaact tccaatcgat
     1801 gcaggcgatt ggatttttaa gtagctttat tccggtatta aaacgacttt ataaagatcg
     1861 tccacaggaa gaacgagcgg aagcaatgaa acgccactta aaatttttta acagccacgt
     1921 taattctgat gcattgattt taggaattgc cgctgcggta gaagagacta ccaaagagga
     1981 tgaaaaagag acagtaaccg cgattaaaac cggtttaatg gggccgttag cagggctggg
     2041 agacagcttg ttgaaattca cctggttacc gatttgcggt agtatcgggg cggcattggc
     2101 actaagtggc aatgtgttag gcccgatttt aatgttcttg ttgtataaca gtgtaaacat
     2161 tgcgacgaag tattacggta tccatctagg ttacagcaaa ggggttgatt taattcacgg
     2221 tgcgggtaat aacattttgc aacgactcgg caatatggca aatgtggttg gtttaatggt
     2281 gattggcgca ttaattgcct ccgttgtgaa agtcaaagta gtttcacaaa ttgccgctgg
     2341 tgataatgtg attaaattcc aagagatgtt agataaagtg atgccgggac tactcagctt
     2401 acttttaact gtcgtgatct actggatttt gaagaaaact aacggcaaat atgctgcaac
     2461 gatgatcctt gctacgatgg ggatctgtat cggcttagtc tatgcaggaa tactcggcta
     2521 atggacagtg tgtatgacat tattcggttg gaacagggca gatacctgtt ccgccaacag
     2581 ccggctcaat cggtacagat tttctttctg gacaaccctg acagtaagga tagcaaatgg
     2641 ctggcggaga ctgatttggc agagtttgag ctgaaattga gcgcttgtga agcccgtccg
     2701 ttcttcctca ttcaatcagc aaacggagaa cagattgttg ctgaacgcac cttacctgtt
     2761 gccggaatga ataattttcg ggatatgggg ggctatgtcg ctcatcaggg caagcgagtg
     2821 aaatggggca agttataccg ctccgatcat ctgcataatt taagagacga aggcgtggct
     2881 tatcttgata aattgggaat tcagactgtt attgactatc gcagcccgaa tgaagtcgcc
     2941 aaatacccaa atcccccaat taatggcaga gagcaaactt ttcggcttga tccgaatgca
     3001 catactgccg agcttgctgc ccaatttagt gcagacaagc acgatgaaga tcgtaatttg
     3061 gtcaataaaa tcattgctca aaaagctgaa ggtaatctta tcaatcgcta cgacattgta
     3121 atggcacaat atcgtaattt tgtcgaaaaa gcagaatgcc aaaccgcctt tgctgaaatg
     3181 ttaaggttgg cgacagaccc tgaaaatgca ccattggttc aacactgccg tggagggaaa
     3241 gatcgcactg gtttcggtgc aatgttactg cttggtattt taggcgtgag taaagctgat
     3301 attattgctg attatatgct tactcattat aaccgccttg ctcgtaacga agagaaaatg
     3361 gcgatttacc gtaccttcac tcaagatcaa gatgtgttgg actacctatt gtctttaatc
     3421 gatactcaac ccgaatttat cgaacaatcg cttaatacta ttgaaaccca atatggtact
     3481 attgagcaat atgcccaaag ggtattaggt attactgcga aggaaattga agcattacgg
     3541 gcaaattatt tggcgtagaa cggtagggtg taatattgaa cacactacaa atggagaaat
     3601 ttcatatgcg ttattttgca aaacattggt ataaaactac cgcttgtctg acaacaattt
     3661 tcacagcatt tcctcttttt gctcaaccaa ttgaaaatat accaacttcc cactacgaat
     3721 tagaaaaaat cattatattt agccgacacg ggttacgttc ccctcttaca gaaaaaggca
     3781 gtgatttaga aaaatcaacc ccttataaat gggcaaattg gagtgtaccg accggtcatt
     3841 taacccataa agggacgctg ttggaaacct attttgggca gtatcttggt caatggctca
     3901 cacataatgg gttattggac aaggcaagct gtgaaagtgg gaagcagatt gaggtttatg
     3961 ctaatagcct gcaacgtaca attggcactg cacaagcttt ggttgccggt gggttttcag
     4021 gttgtaatgt acaagttaaa caccaccagc agattggaaa gatggatccg gtgtttaatc
     4081 caattattcg ctccgatgac cagacatttg tgcaaacagc attaagcaaa attgacctca
     4141 ataaaaccca tcagtattta gcgccatcct atcatttatt aagcgaaata atcgattatg
     4201 ctcattcaga aaaatgtttg caggaaaaac agtgtgaatt ttttaatgat gttggtaaat
     4261 taaggattaa aaaggataaa taaccaggtg tttccggtac gatgcgatta agtaaaaata
     4321 ttgtcgatgc cttattgctg caatattatg aaggttttcc tttaaaagag attgcaaata
     4381 accagcttga tagtgatcag aaatggcaaa cactcaataa aattaaaaac gattacc
//
LOCUS       DANLXF010000191         4434 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.191, whole genome shotgun sequence.
ACCESSION   DANLXF010000191 DANLXF010000000
VERSION     DANLXF010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.191"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..262
                     /locus_tag="PXD66_002061"
     CDS             <1..262
                     /locus_tag="PXD66_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDL6320321.1"
                     /translation="EGEWRYSQKSIDEQLKNGDYYIIKSLDFRPRRVFERSAEKKKIH
                     NLISRAHYDMATNEDSASEHLELFGEDIAEDSFDYPKPEKLI"
     gene            269..340
                     /locus_tag="PXD66_002062"
                     /pseudo
     CDS             269..340
                     /locus_tag="PXD66_002062"
                     /inference="COORDINATES: protein motif:HMM:NF013704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            350..868
                     /locus_tag="PXD66_002063"
     CDS             350..868
                     /locus_tag="PXD66_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDL6320322.1"
                     /translation="MNRRYIGIEIGEHAKTHVVPRLKKVIEGEQGGISKAVNWQGGGS
                     FRFCELGEEVFDAFGSLNPNIRFDDLAAHIWYLENHFPLQKNSEKSPLVGTFNGKAYY
                     LLYNGILGDKRPQSGNVLTRKLMAELPYFAEFIQQGMDIVVYGEACRLGEVQLAEKKI
                     TFKQIPYDVMSR"
     gene            884..1873
                     /locus_tag="PXD66_002064"
     CDS             884..1873
                     /locus_tag="PXD66_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL6320323.1"
                     /translation="MKLKNYQNEALNRVSHYFSACLQSDAKTAFSQIQPEYSYKIPSE
                     HSNLRDVPYVCVRIPTGGGKTLLASHSIARIAKQYLDCDFPVTLWPVPSRTIKTQTTE
                     ALKNPQHPYRAVLDKTFNREVMVIESEDFDLLRPQDFGNKAIVIVSTIQNFRVENQDG
                     RKIYAFNEKLTAHFERLLPHIQANLEKISTTDLQENGLTSKQTGKIKCSFANLLRAYQ
                     PLVIVDEAHNARTSLSFDVLSQLSPSAILEFTATPNTDRKAGSNVLYHVSASHLKAEE
                     MIKLPIVLTEHHNWQQAIDGAVINRGALAQKAQYESEYVRPIYCFKLSRKTAK"
     gene            1840..3069
                     /locus_tag="PXD66_002065"
     CDS             1840..3069
                     /locus_tag="PXD66_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320324.1"
                     /translation="MFQAEPKNGKVTVEVLKQYLIDELKIDEEEIAIVIGNQHELDNI
                     DLFNPQCKINYIITVEALKEGWDCPFAYIFCSVQNVSSSKEAEQLLGRVLRMPYAKHR
                     QIEDLNRAYAPLSSKSFGETARNLQDKLIGMGFEALEVAEMLRIPQQENWIGNEGDLF
                     YTPTTVLALTQLPEMSDLSEAKRSQLKITEQEGHILVQVKGNLSEKLEKALIRTATGK
                     KKEQIEQQLQIHQNRVEILSSPAEKGEIFKVIPQLCMNIQGEFDLADSQVLLDYQSWN
                     LLDYPAKLDGFRLQENSQSFEIDVEDKRVSYHFNQQQLSFSDDWLRNPVKRGFSLPLA
                     HQNFYPDFIVELIDGRILIVEYKGEQLKSNEDSKEKALIGNLWEKLSQGKGLFIMAVK
                     SDENGQDVRTQLLNKLA"
     gene            complement(3142..4422)
                     /locus_tag="PXD66_002066"
     CDS             complement(3142..4422)
                     /locus_tag="PXD66_002066"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005762304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HDL6320325.1"
                     /translation="MSKATLTLETGEKLELNMQKGTLGYQTIDIQPFLKHKLFAYDPG
                     LVSTAVCRSNITYVDGDEGVLLYRGYPIDQLAKHSDYLEVGYMLLFGERPTKEEYKDF
                     VQLIKKHTLVHEQLTKFFSGFRRDSHPMAVMCGVSGALAAFYHDAIDVNNQAHRELTA
                     IRLLAKIPTLAAMCYKYSIGQPFMFPQNHLSYAGNFLYMMFATPCEPYEVNPVLERAL
                     DRIFILHADHEQNASTSTVRTAASSGANPFACIAAGIASLWGPAHGGANEACINMLKE
                     IGTVDRIPEFIARAKDKNDPFRLMGFGHRVYKNYDPRAKVMRETCHEVLKELNIDNPL
                     FEVALELERIALSDPYFIEHKLYPNVDFYSGIVLKAIGIPTSMFTVMFALARTVGWIA
                     HWKEMYLQGNIKIVRPRQIYTGHTKRDFEPMDKS"
BASE COUNT         1418 a          773 c          944 g         1299 t
ORIGIN      
        1 tgaaggtgaa tggcggtata gccaaaaaag tattgatgag caattaaaaa atggtgatta
       61 ctatattata aaaagtttag actttcgacc aaggagagta tttgagcgaa gtgcggaaaa
      121 gaaaaaaatt cataacttaa ttagcagagc tcattatgat atggcaacaa atgaagactc
      181 tgctagtgaa catctagaat tatttgggga ggatattgca gaagattcat ttgattatcc
      241 taaacctgaa aaattaatct aatttgtatt agaagcatcg actgaaccat atgatctcgt
      301 cctcgatagc tttcttggct caggtacaac tgcagcagtt gctcataaaa tgaaccgtcg
      361 ttatatcggt attgagattg gcgaacatgc taaaacccat gttgtcccac ggttgaaaaa
      421 agtaattgaa ggtgagcaag gcggtatttc aaaagcagta aattggcaag gtggtggatc
      481 gtttcgcttt tgtgagttag gcgaggaagt atttgacgca ttcggttcac ttaatccgaa
      541 cattcgtttt gacgatttag ccgcccatat ttggtattta gaaaatcatt tccctttgca
      601 aaaaaacagc gaaaaatcac cgcttgtcgg cactttcaac ggcaaagcct actatttgtt
      661 atataacgga attttaggcg ataaacgtcc gcagagtggt aatgttctga cacgaaaatt
      721 aatggcggag ttaccatact ttgctgaatt tattcaacag ggaatggata ttgtggtata
      781 tggcgaagcg tgccgtttag gagaagtgca gttagctgaa aagaaaatta catttaaaca
      841 gattccatat gatgtaatgt ctcgttaata gtaggagaaa aaaatgaaat taaaaaatta
      901 ccaaaatgag gcattaaacc gtgtcagcca ctatttttcg gcttgtttgc aatctgatgc
      961 aaaaaccgca tttagccaaa ttcaacctga atatagttac aagatcccaa gtgaacacag
     1021 taatttgcgt gatgtgcctt atgtttgtgt acgaattcca actggtggcg gtaaaacgtt
     1081 gttagcctcg catagtattg ctcgtattgc aaagcagtat ttggattgcg atttcccggt
     1141 taccttgtgg ccagtgcctt ctcgtacaat taaaacccaa actacagaag cattaaaaaa
     1201 tccgcaacac ccttaccgtg cagtacttga taaaactttt aaccgagaag taatggttat
     1261 tgagtctgaa gattttgatt tacttcgccc acaagatttt ggtaataagg cgattgtaat
     1321 cgtttctact attcaaaatt ttcgggtaga aaatcaagat gggcgaaaaa tctatgcttt
     1381 taatgaaaaa ttaacagctc attttgagcg tttactaccc catattcagg caaatttaga
     1441 aaagatttcc actactgatc tacaagagaa cggtttaacc tccaaacaaa cgggcaaaat
     1501 taagtgttct tttgcaaatt tgttgcgagc ttatcagcct ttggtcattg ttgatgaagc
     1561 acataatgct cggacttcac tctcttttga tgtgctatct caactttccc catcagctat
     1621 tttagaattt actgctacgc caaacacaga tagaaaagca ggaagtaatg tgttgtatca
     1681 cgtttcggca agccatctaa aagcagaaga gatgatcaaa ttgcctattg tgctgaccga
     1741 acatcacaac tggcaacaag caatagatgg ggctgtaatc aatagaggag cattagcaca
     1801 aaaagcacag tatgagagcg agtacgttcg tccgatttat tgtttcaagc tgagccgaaa
     1861 aacggcaaag taacagtaga agtcttaaaa cagtatttaa ttgatgagtt aaaaattgat
     1921 gaagaagaga ttgccattgt gataggtaat caacacgaat tagataatat cgatttattt
     1981 aatccacaat gcaagatcaa ttacatcatc acggttgagg cattaaaaga agggtgggat
     2041 tgcccttttg cttatatttt ttgctcagta caaaatgttt cctctagcaa ggaagcggag
     2101 cagctattag ggcgagtatt gcgaatgcct tatgctaaac accgtcaaat tgaagattta
     2161 aatcgtgctt atgctccgct ttcctctaag tcgttcggcg aaacggctcg taatttacaa
     2221 gataaattga ttggaatggg gtttgaagca ttagaagtag ctgaaatgct tcgtatccca
     2281 caacaagaaa attggattgg aaacgaagga gatttattct atacaccgac aacagtttta
     2341 gcattaacgc aactccctga aatgtctgat ttaagcgaag caaaacgttc tcaacttaaa
     2401 attaccgagc aagaaggtca cattttagtg caagtaaaag gtaatttaag tgaaaagtta
     2461 gaaaaagcgt tgattcgaac tgcaacaggt aagaaaaaag agcaaattga gcaacagtta
     2521 caaattcacc agaatagagt tgaaatatta tcttctcctg ccgaaaaagg ggaaatattt
     2581 aaagtaatac ctcaactgtg tatgaatatt caaggcgaat tcgacttagc tgattcacaa
     2641 gtgctattag attaccaaag ttggaattta ttggattatc cagccaaact tgatggattt
     2701 agattacaag aaaatagcca aagttttgaa attgatgtgg aagataaacg ggtttcttac
     2761 cattttaatc agcagcaact gagtttttct gatgattggt taagaaatcc tgtaaaaaga
     2821 gggttttctt taccattagc tcatcagaat ttttaccctg attttattgt agagcttatt
     2881 gatggtcgaa ttttaattgt ggaatataaa ggggaacaat taaaaagtaa tgaagattct
     2941 aaagaaaaag ccttaattgg caatctttgg gagaaattaa gtcaaggaaa aggcttattt
     3001 attatggctg taaaaagcga tgaaaatggt caagatgtta gaacacaact gctaaataaa
     3061 ttagcctgat ttgcaaattt tatgcaaaat ataaccgctt gtggaaacaa gcggttaaaa
     3121 tttttcgatt ttttgccaat cttatgattt atccattggc tcaaaatcac gtttagtatg
     3181 tccggtgtag atttgacgtg gacgaacgat tttgatattg ccttggagat acatctcttt
     3241 ccagtgagca atccagccaa cggttctggc tagggcaaac attacggtaa acattgaggt
     3301 tggaataccg atggctttta gcacgattcc ggagtagaaa tctacgtttg gataaagttt
     3361 atgctcaatg aaataagggt cgcttaacgc aatacgttct aattccaatg ccacttcaaa
     3421 tagtgggtta tcaatgttca gttcttttaa cacttcgtgg caggtttcac gcattacttt
     3481 cgcacgaggg tcgtagtttt tatagacacg atgcccaaag cccattaagc ggaacgggtc
     3541 atttttatct ttggctcgag caataaattc aggaatgcga tccacagtgc cgatttcttt
     3601 caacatattg atacaggctt catttgctcc tccgtgtgca ggtccccaga gagaggcaat
     3661 accggcagca atgcaagcaa atgggtttgc acctgatgaa gcggcggttc gcacggttga
     3721 ggtcgaagcg ttttgttcgt ggtctgcatg taaaatgaaa attcggtcta acgcacgctc
     3781 taacacaggg ttcacttcgt acggttcaca aggagtggca aacatcatat ataagaagtt
     3841 gccggcgtaa gataagtggt tttgtgggaa cataaacggc tgaccgatag agtatttata
     3901 acacatcgcc gcaagggttg ggattttggc taacaaacga atggcggtta attcacggtg
     3961 ggcttggttg tttacatcaa tggcgtcgtg gtagaaggca gccaaagctc cacttacccc
     4021 acacatcacc gccattgggt gcgaatctcg gcggaaaccg gagaagaatt tggttagttg
     4081 ttcgtgtaca agagtgtgtt ttttgatgag ttgaacgaag tctttgtatt cttctttggt
     4141 agggcgttcg ccgaataaca gcatataacc gacttctaag taatcagaat gtttggctaa
     4201 ttggtcaatc gggtagcctc gatagagtaa aaccccttca tcgccatcaa cataagtgat
     4261 attggatcgg catacagcgg tcgaaaccaa tccgggatca taagcaaata acttatgctt
     4321 taagaaaggc tgaatatcaa tcgtttgata ccctaaggtt cctttttgca tattgagttc
     4381 aagtttttca cctgtttcga gtgttaatgt tgcttttgac atagcttact ccta
//
LOCUS       DANLXF010000192         4423 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.192, whole genome shotgun sequence.
ACCESSION   DANLXF010000192 DANLXF010000000
VERSION     DANLXF010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.192"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..896
                     /gene="zipA"
                     /locus_tag="PXD66_002067"
     CDS             <1..896
                     /gene="zipA"
                     /locus_tag="PXD66_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HDL6320326.1"
                     /translation="HIIFFILAGLLIAVLIGFSLWSARREKSNIFSNTFSTRPIAVPP
                     QNNKMSNSIPTSLQPQNEDYQAMSVPEENPQQIQQEVESSLQNIKISLPGGNPQQQPE
                     QIQPIQSVAEPTVSTYGQKISNQQHIQYNTPDFANPMVNTVVEETPQAVAIEPQETQS
                     AEEENPFVTLYLYAPQNEAFRGDFVVHYLEEVGLRFGEHQIFHRHQHIDDSLSPIIFS
                     AANLMNPGTFDLNNISNFATAGLVLFMQLPTEGNDVANMKLLISNAEHLAASLGGFLY
                     DDNQQPFGEESRQRYMQRVMH"
     gene            961..3018
                     /gene="ligA"
                     /locus_tag="PXD66_002068"
     CDS             961..3018
                     /gene="ligA"
                     /locus_tag="PXD66_002068"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDL6320327.1"
                     /translation="MSLFNQTSLFEENATPELVEKIETIRQTLRQYEYEYHVLDNPSV
                     PDAEYDRLMNELKNLEWQHPELISADSPTQRVGAKPLAGFAQVTHEIPMLSLDNAFSD
                     EDLDGFLRRMESYITASPSQLEFCCEPKLDGLAVSILYENGILTRAATRGDGTTGEEI
                     TANIRTIRNIPLKLNTTNPPARLEVRGEVFMPQSGFNELNEKALAKGEKTFANPRNAA
                     AGSLRQLDPKITRQRPLVLNAYGIGVYESDDELPATHFERLQWLKSLGIPVNNEIRLV
                     QGKAALLAYYAEIQAKRPTLGYDIDGTVLKVNDIALQEQLGFISRSSRWAIAYKFPAQ
                     EEMTILKEVDFQVGRTGAITPVAKLEPVFVAGVTVSNATLHNGDEIERLGIVIGDTVI
                     IRRAGDVIPQITGVMLERRPENAKKIEFPTACPICHSAVTRVEGEAVARCTGGLFCEA
                     QRKEALKHFVSRKAMDIDGVGEKLIEQLMARELIETPADLFKLDHTTLMRLERMGEKS
                     AQNALNSIEKAKNTTLPRFLFSLGIREVGEATALNLANHFGSLEAIRQATFEQLKEVQ
                     DVGEVVANRIVSFWQEPHNVEVVEDLIAQGVRWENVVKAEIVDNPLKDKNVVLTGTLT
                     QLTRDEAKALLQSLGCKVSGSVSSKTDYLIAGEKAGSKLAKAQELGIQILSEQQFIEL
                     TQH"
     gene            complement(3081..4349)
                     /locus_tag="PXD66_002069"
     CDS             complement(3081..4349)
                     /locus_tag="PXD66_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HDL6320328.1"
                     /translation="MTQQIEHSTWQSKFAAMGPGILMASAAVGGSHIIASTQSGAIYG
                     WQLAIIILLANLFKYPFFRFGVQYTLASGKTLLEGYKEKGKFYLIIFFILNVFAAMIN
                     TAAVAIVSAAILKFIFSALGIPLDLSIPMAGTIVIVVTWGILLLGQYKFLDTLSKWIM
                     IALTISTVSAVIIAALKGGVEIAPDFVEPSPWNLAALVFIVALMGWMPAPIEISAINS
                     MWVIAKKRLSKVNYEDGIFDFNVGYIGTAILAIVFLALGALVQYGSGEKVEQAGVAYI
                     AQLIKMYAFAIGDWSKLLIAFIAFMCMFGTTITVIDGYSRANAEALRLISGRKQHSQT
                     ELSIWMTVTALIGILIISQFMSDVAKMLQFAMISSFVSTPVFAWLNFSLVLKGEHRAK
                     GWLFWLAVIGLIYLTSFTLLFLAQQFKLIG"
BASE COUNT         1415 a          993 c          882 g         1133 t
ORIGIN      
        1 tgcatattat tttttttatt cttgccgggc tactgattgc tgtactaatt ggatttagcc
       61 tttggtccgc tcgtcgtgaa aaatcaaata ttttttcaaa tacattctcg accagaccta
      121 ttgctgtgcc accacagaat aataaaatga gcaatagcat cccaacctca ttacagccac
      181 aaaatgagga ttatcaagca atgtctgtgc cggaagaaaa tccgcaacag atacaacagg
      241 aagttgaaag ctcgctgcaa aatatcaaaa tcagtttgcc gggcggaaat cctcagcaac
      301 agcctgagca aattcagccg atacaatcgg tagcagaacc aacggttagc acttacggtc
      361 agaaaatttc taatcagcaa catatccaat acaatacgcc ggattttgca aacccaatgg
      421 tgaacacagt ggtggaagaa acacctcaag cagtagctat cgaacctcaa gaaacccaat
      481 cggcagagga agaaaatcca tttgtaaccc tctatcttta cgcaccacaa aatgaagctt
      541 tccgcggtga ttttgtagta cattatttag aagaagttgg cttacgtttt ggcgaacacc
      601 aaattttcca ccgccatcaa catattgatg attcattaag tccgattatt ttcagtgcag
      661 caaatttaat gaacccagga acctttgatt taaataatat ctcaaacttt gcgacagctg
      721 gtttagtgct atttatgcaa cttccaaccg aaggcaatga tgtggctaat atgaagttgt
      781 taatcagtaa cgctgaacac ttagcagcat cgttaggggg attcttatat gacgataatc
      841 aacagccttt tggcgaagaa tcccgccaac gttatatgca acgtgtgatg cactaattgc
      901 gaaaaaatta ataaatccga ccgcttgtac aagcggtctt ttttgtagag aattttacca
      961 atgtctttat ttaaccaaac ctctttattt gaagagaatg ccacgcccga gttagtcgag
     1021 aaaatcgaaa ctattcgcca aacattgcgt caatacgagt atgaatatca cgttttagat
     1081 aacccaagcg tacccgatgc cgaatacgac cgtttaatga acgagctgaa aaatctggaa
     1141 tggcagcacc cggaactgat tagtgccgac tcgccgactc aacgggttgg tgccaaaccg
     1201 ctggcaggct ttgctcaagt cacccacgaa atccctatgc tttcgttgga taatgccttc
     1261 tccgatgaag acttagatgg ctttttacgc cgtatggaaa gctatatcac cgcctctcct
     1321 tcgcaactgg aattttgttg cgaacctaaa ttagacggct tggcagtgag tattttatac
     1381 gaaaacggta tacttacccg agctgcaacc cgtggcgatg gcacaaccgg cgaagaaatt
     1441 accgctaata tccgcaccat tcgcaatatt ccgttaaagc tcaacacgac taatccacct
     1501 gctcgattag aggtgcgagg cgaagtcttt atgccacaat caggctttaa tgagctgaat
     1561 gagaaagcct tagctaaagg cgaaaaaacc tttgccaacc ctcgcaatgc cgcagcaggt
     1621 tcgcttcgcc agttagatcc gaaaatcacc cgccagcgtc cgttagtgct gaatgcctac
     1681 ggaattgggg tgtatgaaag cgatgacgaa ctgccagcca cccatttcga acgtttgcaa
     1741 tggctgaaat cgcttggtat tcctgtaaat aatgaaatcc gcttggttca aggcaaagct
     1801 gctctcttgg cttattatgc ggagattcag gctaaacgcc caacattagg ctatgatatt
     1861 gatggcacag tattgaaagt aaatgacatt gccctacaag agcaactcgg gtttatctct
     1921 cgctcctctc gttgggcgat tgcgtataaa tttccggctc aagaagaaat gacaatcctc
     1981 aaagaagtgg acttccaagt gggaagaaca ggggcaatca cccctgtggc gaaattagag
     2041 cctgtttttg tcgctggcgt aacggtaagt aacgcaacgc tacacaacgg tgatgaaatc
     2101 gagcgacttg gtattgtgat tggcgatacg gtaattattc gccgagcggg cgatgtaatt
     2161 ccgcaaatta caggcgtgat gttagaacgc cgcccggaaa atgcaaaaaa aattgaattt
     2221 ccgaccgctt gtcctatctg tcactccgct gttactcggg tagaaggcga ggcagtagca
     2281 cgctgtaccg gtggtttatt ctgcgaagct caacgtaaag aagccttgaa acatttcgtt
     2341 tcacgcaagg caatggatat tgacggcgta ggcgaaaaac taatcgagca gttaatggct
     2401 cgagagctga ttgaaacgcc tgccgatctg tttaagctcg atcacactac cctaatgcga
     2461 ttagagcgaa tgggggaaaa atcggctcaa aatgctctca acagcatcga aaaagcaaaa
     2521 aacactactc ttcctcgctt tttattctcg ttaggtattc gtgaagtggg cgaggcaacc
     2581 gctctcaact tagccaacca ttttggtagc ttagaggcga tccgccaagc cacttttgag
     2641 caattaaaag aagtgcaaga tgtgggcgag gttgttgcca accgtattgt cagcttctgg
     2701 caagaaccgc ataatgtgga agtggtggaa gatttgatcg ctcaaggtgt acgttgggaa
     2761 aacgttgtga aagccgaaat tgtagataac ccgttaaaag ataaaaacgt ggtcttaacc
     2821 ggcacgctaa cccagctcac ccgtgatgaa gccaaagccc tattacaaag tttgggctgt
     2881 aaagtctccg gctcggtttc cagcaaaacc gattacttaa tcgccggcga aaaagccggt
     2941 tccaagctgg caaaagctca agagttggga attcagattt taagcgagca acaatttatt
     3001 gagcttactc aacattaaca aacaaagggg cagatgattg aaatctgccc cttattctat
     3061 ctattgaatg cttaaaagca ttaaccaatc aatttaaact gctgagctaa aaacagtaac
     3121 gtaaagctag ttaagtaaat taagccgata actgccaacc aaaataacca gccttttgca
     3181 cgatgctcac cttttagcac taaagagaaa tttaaccacg caaaaacagg ggtagaaaca
     3241 aatgatgaaa tcattgcaaa ttgtaacatt ttcgccacat cactcataaa ttggctaata
     3301 attaaaatgc cgatgagtgc agttaccgtc atccaaatac ttaattctgt ttgtgaatgt
     3361 tgcttacgcc cgctaattaa acgcaacgct tctgcattcg ctcgtgaata tccatcaata
     3421 acagtgatcg ttgtgccgaa catacacata aatgcaataa atgcgattaa aagcttagac
     3481 cagtcaccaa tcgcaaaagc atacattttg attaattgag caatataggc aacaccggct
     3541 tgttctactt tctcacctga accgtactga actaacgcac ctaatgctaa aaaaacaatc
     3601 gccaaaattg ccgtgccgat ataacccaca ttaaaatcaa aaataccgtc ctcataattc
     3661 actttactta aacgtttttt cgcaataacc cacattgagt taatggcaga aatttcaatt
     3721 ggtgcaggca tccagcccat taatgcaacg atgaacacaa gtgctgctaa attccacggc
     3781 gaaggctcta cgaaatctgg agcaatttca acgccacctt ttaaagcagc aatgatcacg
     3841 gcagataccg tagagatggt taaagcaatc ataatccatt tagataatgt atctaagaat
     3901 ttatattgcc ctagaagtaa aataccccaa gtgaccacaa tcacaatagt tcctgccatt
     3961 ggaatgctta aatctaacgg aatacctaaa gcagaaaaaa taaattttag aatcgctgct
     4021 gaaacaatcg ccaccgcagc tgtgttaatc attgcagcaa aaacgttaag gataaagaaa
     4081 ataataagat aaaactttcc tttttctttg tagccttcca ataaagtttt accgctagct
     4141 aaggtatatt gcacaccaaa gcggaaaaac ggatatttaa ataaattggc taacagaata
     4201 ataatcgcca gctgccagcc ataaatcgca ccagattgtg tagaggcaat aatatgcgaa
     4261 ccacccactg ccgcagatgc catcaaaatc cccggcccca ttgcagcaaa tttactttgc
     4321 caagttgaat gttctatttg ttgtgtcata tagctttcct tatcacatta ctatcaaaac
     4381 aagatggttt cattctactt ataaaaacca aataaaaaca ctc
//
LOCUS       DANLXF010000193         4412 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.193, whole genome shotgun sequence.
ACCESSION   DANLXF010000193 DANLXF010000000
VERSION     DANLXF010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.193"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(138..755)
                     /locus_tag="PXD66_002070"
     CDS             complement(138..755)
                     /locus_tag="PXD66_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3164 family protein"
                     /protein_id="HDL6320329.1"
                     /translation="MSKVTIGNEIYWKDADGNLKPEALVKEIDKERDELVRQFVSKAQ
                     ALSKALGDFKQSVFDDVGAFVSLSADKYGVKIGGAKGNITLFTYDGEYKLQLAVQENI
                     RFDERIHAAKALIDECLHDWSEGAKPELKALIDNAFEVDKEGNLSTAKILSLRRVEID
                     DARWNQAMTAISDSVQVIGSKGYIRFYKRDENGKYQPISLDMAGV"
     gene            complement(768..959)
                     /locus_tag="PXD66_002071"
     CDS             complement(768..959)
                     /locus_tag="PXD66_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320330.1"
                     /translation="MKKRRMIYALYVGEENVADGTAEELAERLGVEAKTVRFMSSPAH
                     NKRVKYNGKAIRTVKVGLE"
     gene            complement(962..1282)
                     /locus_tag="PXD66_002072"
     CDS             complement(962..1282)
                     /locus_tag="PXD66_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320331.1"
                     /translation="MQKVYSEKAGEMMNPLNAFIHDSLVKLEIATLECEAMELEVERV
                     EFEAKSPPRLVLRDNCKTQDLVKNGLASLFGMKTKGGRRYDSYQMQVRGVKCVWESER
                     VTKV"
     gene            complement(1293..2174)
                     /locus_tag="PXD66_002073"
     CDS             complement(1293..2174)
                     /locus_tag="PXD66_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL6320332.1"
                     /translation="MKNQELRALMDSKGYQQKQVAQLLGVSVATVSLYLKGDYNGNVA
                     EMDRKVDELIERDKAKVVEAKYNAAFVPTLAARRGMEVMQFAHIEGEINVIFGAAGLG
                     KTQMLKEYERRNSSATLIEVDPSCTPKVLLRKIAEAVGANARGVNNELLESIVNKLKG
                     SERLLMIDEAELLSTRSLEFIRRIHDLTSVGVVLAGMPRLLVNLKGKNNELAQLYSRV
                     GFACDLGNALSEEDLGLLAESALGTNEFNAPLIKACHGNARRLSKLMRGVVRSSEINQ
                     TPISKDLVEQYSKMLIS"
     gene            complement(2178..4115)
                     /locus_tag="PXD66_002074"
     CDS             complement(2178..4115)
                     /locus_tag="PXD66_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL6320333.1"
                     /translation="MKDWFSAKELAGISGLSKYPTNVTRQAKKEQWATRPLKGVKGGG
                     FEYHINSLPTEVQTEIRSRFMSAVVEAKPKKLPVVRAEVDVGNLTTKQREIADARMAL
                     VAYVLELEGSMSRIKAITYLCNLAKQGEMPPHLAELVAVANAKKTAKRTLSVRTLNGW
                     VVDYCKAENAEQRLKLLAPQVRQETKPEEIWWLSAFLGVYRQKNGICLTEAYREFEVE
                     WAYQYADNPLLLAQCPSLSQVQRAMNKLPLYVKEYGRRTGSHYKQLLSYVKRDWSVLR
                     ANDVWIGDGHSLKLKVAHPIHGKPFTPELTMIVDGAGRKVVGWSLALSENAFAVADAL
                     RHAISLHGVPAIYYSDNGGGEKNKFLDAEITGMLPRLGIRHETGIAGNPQGRGIIERL
                     NKTIGMTIARQFETGYATGADPETVRKTLYAVNSLANAKGELTPLQRKAQGKLPTWQQ
                     LIEVIQSVIDWYNNEHIHSEIRTTPARKYQQMLHAEDVVMLSPIELRDMSRPEFIRKP
                     DRGWVSWNNNHYFNLKLLDFDREEVVIGIDIHNAESVQVRTKDGRFICEAIWNGNTRE
                     AFPVAMVEQQRKERHKRRANLKQQQLDEINAELNPVLTIEQKNDFSLLATARKPKKEV
                     EPIFLTRAEKEDYEKKLAVGG"
     gene            complement(4130..4357)
                     /locus_tag="PXD66_002075"
     CDS             complement(4130..4357)
                     /locus_tag="PXD66_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL6320334.1"
                     /translation="MNKKKRAQDMHRADIRAELMKKGITFSQLGIENGLSKTTVRNAL
                     DKPYKNGEEIIAKALGKTPAEIWPSRYPDVA"
BASE COUNT         1025 a         1176 c          813 g         1398 t
ORIGIN      
        1 agtccatcat tattaatttt ttgttatcac aaaagtgtac tgttacattc ccgttaatat
       61 tttctattga acaaaccttg tctgtattaa ccacaacagg tttatccgta taattaacat
      121 taaaagtaat aaacattcta aacccccgcc atatctaaac taatcggctg atactttccg
      181 ttttcatcac gtttataaaa acggatataa cctttactac cgatcacttg tacgctatct
      241 gaaatcgctg tcattgcttg attccatctt gcatcatcaa tctcaacacg gcggagagag
      301 agaatttttg ctgtagataa attaccttcc ttatccacct caaacgcatt atcaattaag
      361 gctttaagct ccggttttgc cccttccgac caatcgtgca agcattcatc aatcaaggct
      421 tttgctgcgt gaatacgctc atcaaagcga atattctctt gcactgctaa ctgcaactta
      481 tattcaccgt cataagtaaa tagtgtgata ttgccttttg caccgccaat ttttactcca
      541 tatttgtcag ccgataggct gacaaaagcc cccacgtcat caaatacgga ttgtttaaaa
      601 tcacctaacg ctttgcttaa cgcttgtgct ttgcttacaa attggcgaac taattcatca
      661 cgttctttgt cgatttcttt tactaatgcc tccggcttta aattgccatc agcatctttc
      721 cagtaaattt cattaccgat tgttacttta ctcataatac ttcctctcta ttccaacccg
      781 actttcacgg tgcggattgc tttaccgtta tatttcactc ttttgttgtg agcaggggac
      841 gacataaacc gcactgtttt cgcctccacc cccaaccgtt ctgctaactc ttccgccgta
      901 ccgtcagcca cattctcttc gccaacatac agggcataaa tcattcgccg ttttttcatt
      961 tctacacctt cgtaacacgt tcagactccc acacgcattt caccccccgc acctgcattt
     1021 gatagctgtc ataccgtctg ccgcctttgg ttttcattcc aaacaggctt gccaagccgt
     1081 tcttgactaa atcctgtgtt ttgcagttat cccgtaagac caagcggggt gggcttttcg
     1141 cttcaaactc cactcgttca acttccaact ccatcgcctc acattccaaa gtggcaattt
     1201 ctaacttcac aaggctgtcg tggataaacg catttaaggg gttcatcatt tcgcctgctt
     1261 tttcgctgta aaccttttgc ataacttgct ccttagctaa tcaacatctt gctatattgc
     1321 tcaaccaaat ctttactaat tggtgtttgg ttaatctcgc tagaccgcac cacgccacgc
     1381 attaacttac ttaatcggcg ggcattgccg tgacaagctt taattagcgg ggcattaaat
     1441 tcatttgtac ctaacgcact ttcggctaat agccccaaat cttcctcgct caaggcattt
     1501 cccaaatcac aagcaaaacc tactcggcta taaagctgtg ccagctcatt atttttacct
     1561 ttaagattaa ccaacaatct cggcataccg gctaacacca cgcccacgct ggttaaatcg
     1621 tggattcggc ggataaactc cagcgagcgg gtcgaaagta attcagcttc atcaatcatt
     1681 agcaaccttt cagagccttt taacttgttc acaatgcttt ctaatagttc gttatttacc
     1741 cctctggcat ttgcccccac cgcctcggca atcttgcgga gcaagacttt aggtgtgcaa
     1801 ctcggatcaa cctcaattaa tgttgcagag ctgtttctgc gttcgtattc tttgagcatt
     1861 tgggttttac ctaagccggc tgccccaaaa atcacattaa tttcgccttc aatatgggca
     1921 aactgcatta cctccattcc tctgcgtgcc gccaaagttg gcacaaaagc agcgttatat
     1981 ttcgcttcca ccacttttgc cttatcacgc tcaatcagct cgtctacttt gcgatccatt
     2041 tcagccacat tgccgttata atcgcctttg agatacagac tgactgtggc aacggataca
     2101 cctaaaagct gtgccacttg tttttgttga tagcctttgc tatccattaa ggctctaagt
     2161 tcttggtttt tcatcgttta gcctcccact gctaactttt tttcgtaatc ctctttttct
     2221 gctcgggtca aaaaaatcgg ttctacttct tttttcggtt ttcttgccgt tgccagcaag
     2281 ctaaaatcgt ttttctgctc aatggttaaa accgggttta attccgcatt aatctcatca
     2341 agctgttgct gtttaaggtt ggctcggcgt ttatggcgtt ctttacgctg ctgttctacc
     2401 atcgccaccg ggaatgcctc tcgggtgtta ccgttccaaa ttgcctcgca aataaacctg
     2461 ccgtctttgg tacgcacctg cacgctctcg gcattgtgaa tatcaatccc gatcaccact
     2521 tcttcacggt caaaatccag taatttcaag ttaaaatagt ggttgttatt ccaacttacc
     2581 caacctcgat ccggcttacg gataaattcc ggtcggctca tatcccgcaa ctcaataggg
     2641 cttaacatca ccacatcttc tgcgtgcaac atctgctgat atttgcgtgc cggcgtggta
     2701 cggatttccg agtgaatatg ctcgttgttg taccagtcaa tcaccgattg gattacttca
     2761 atcaactgtt gccacgttgg caatttgcct tgggctttac gttgtagtgg ggttaattcc
     2821 cctttggcat ttgccagtga gttcaccgca tacagggttt tgcgaacggt ttccggatct
     2881 gcaccggttg catagccggt ttcaaactgt cgggcaatcg tcataccgat ggttttattt
     2941 aagcgttcaa taatgcctcg cccctgcgga ttgcccgcaa tccctgtttc gtgtcggata
     3001 ccgaggcggg gcaacatccc cgtgatttcc gcatccaaaa atttattctt ttcaccacca
     3061 ccgttatccg agtagtagat tgccggcaca ccgtgtaggc taatggcgtg gcgtagtgca
     3121 tcagccaccg caaaggcgtt ctcactgagt gccagcgacc agcccaccac ttttctacca
     3181 gcaccatcga caatcattgt cagctcaggg gtaaacggct taccgtgtat cgggtgggct
     3241 actttgagct tcagtgagtg accgtcacca atccatacat catttgctcg aagtaccgac
     3301 caatcccgct tcacatagct cagtaactgc ttgtaatgcg agccggtgcg tctgccgtac
     3361 tctttcacat acaacggcaa cttattcatc gctcgctgta cttggcttag gctggggcat
     3421 tgtgccaaca gtagcgggtt gtctgcatac tgatacgccc attccacctc aaattctcgg
     3481 taggcttcgg tgaggcaaat accgtttttc tgccgatata cgcctaaaaa agcacttagc
     3541 caccaaattt cttccggctt ggtctcctgc cgtacctgtg gggcaagcag tttcaaccgt
     3601 tgctcggcgt tctctgcctt gcagtaatcc accacccagc cgtttaaggt tcgcaccgag
     3661 agtgtgcgtt ttgccgtctt tttagcattt gctacggcaa ccagctccgc caagtgcggt
     3721 ggcatttcgc cttgtttggc aaggttgcaa aggtaggtta tcgctttaat ccggctcatt
     3781 gagccttcca actccaacac atacgccacc agtgccattc gtgcatcagc aatttctcgc
     3841 tgtttggtgg tcaggtttcc cacatccacc tctgcacgca ctaccggtaa ctttttcggc
     3901 ttggcttcca ccaccgccga cataaagcgg gagcggattt cggtttgaac ttcggtgggt
     3961 aaagagttaa tgtgatattc aaaaccacct cctttaaccc ctttcaatgg acgagttgcc
     4021 cattgttctt ttttggcttg tctagttaca tttgttgggt attttgataa gccactaata
     4081 cctgctaatt cttttgcact aaaccaatct ttcataacag ctatccttgt tatgcaacat
     4141 cagggtaacg actaggccat atttcggctg gcgtttttcc taatgctttt gcgataattt
     4201 cttcaccgtt tttataaggt ttatcaagag catttcttac tgttgttttg gaaagcccat
     4261 tctcaatccc taactgagaa aatgttattc ctttcttcat caattcagct cgaatatcag
     4321 ctctatgcat atcttgagct cttttctttt tattcatttt gtgagatcct ccacatttag
     4381 tttatttgct acgctttagc gtacttgatt aa
//
LOCUS       DANLXF010000194         4337 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.194, whole genome shotgun sequence.
ACCESSION   DANLXF010000194 DANLXF010000000
VERSION     DANLXF010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.194"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            63..1481
                     /gene="leuC"
                     /locus_tag="PXD66_002076"
     CDS             63..1481
                     /gene="leuC"
                     /locus_tag="PXD66_002076"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDL6320335.1"
                     /translation="MQQAKTLYQKLFDSHVVYEAEGETPIIYINRHLIHEVTSPQAFD
                     GLRVAGRQVRQVSKTFGTMDHSISTQVRDVQQLEGQAKIQVLELEKNCEETGIPLFDI
                     TKKQQGIVHVMGPEQGLTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTL
                     KQARAKSMKVEVRGKVNPGITAKDIVLAIIGKTTMAGGTGYVVEFCGEAIRDLSMEGR
                     MTVCNMAIEFGAKAGIVAPDETTFAYLKDRPNAPKGQDWEDAIEYWKTLKADEGAVYD
                     AEVILEAKDIAPQVTWGTNPGQVIGIDDVIPNPEDMADPVTRASAEKALAYIGLEAHT
                     DMKDVPVDQVFIGSCTNARIEDLRAAAAVMKGRKKADNVKRILVVPGSGLVKEQAEKE
                     GLDKIFIEAGAEWRNPGCSMCLGMNDDRLGEWERCASTSNRNFEGRQGRNGRTHLVSP
                     AMAAAAAVFGKFMDIRKVSLNA"
     gene            1494..2096
                     /gene="leuD"
                     /locus_tag="PXD66_002077"
     CDS             1494..2096
                     /gene="leuD"
                     /locus_tag="PXD66_002077"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDL6320336.1"
                     /translation="MAGIKQHSGLVVPLDAANVDTDAIIPKQFLQAITRVGFGKHLFH
                     EWRYLDAEETQINPDFVLNFPQYQGATILLARKNLGCGSSREHAPWALADYGFKVMIA
                     PSFADIFYNNSLNNHMLPIKLTEEEVEEIFQWVWANEGKQIHVDLEAMTVTVGEKVYT
                     FELDEFRRHCLLNGLDNIGLTLQHEAAIAAYEKNIPAFLR"
     gene            complement(2168..2632)
                     /locus_tag="PXD66_002078"
     CDS             complement(2168..2632)
                     /locus_tag="PXD66_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320337.1"
                     /translation="MNKFKIALMATVAIGLFACTPSPQSNSTKQQAQVGYLKQNISQA
                     ELNNPKDFKRYSYYCKNFATGSSSYLATYFPLWSESRKQENFGIYFQLDGGKVYPFDH
                     LANISLNARATKFEVRYRSYQPIEGSFVELVAREFTSTYYKNNVPWLECREG"
     gene            complement(2656..3162)
                     /locus_tag="PXD66_002079"
     CDS             complement(2656..3162)
                     /locus_tag="PXD66_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320338.1"
                     /translation="MADPHIQSPMDFWDYLTVIIYRSGFVLATLMLLLLPYYPQTAQY
                     GLLVSGTMLASSLHLYAKSFRLIFQFSVWIGLLGAVFNFPLLAFGAMLLVIGGLSYKE
                     YFCFRVFGLNFQPLLVAVLWIAFALEWSLLVQILSLVCGLLLLVLSIQKWRMPLHFDI
                     GDKSKYQI"
     gene            complement(3185..4162)
                     /gene="dusA"
                     /locus_tag="PXD66_002080"
     CDS             complement(3185..4162)
                     /gene="dusA"
                     /locus_tag="PXD66_002080"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HDL6320339.1"
                     /translation="MTNEIQPFYRGRFSVAPMLDWTTRHCRYFHRQFSRQALLYTEMI
                     TAPAIIHAKYDLLEYDPSENPVALQLGGSDPAQLAHCAKLVAERGYAEINLNVGCPSD
                     RVQNGMFGACLMAKADLVADCIKAMQDAVEIPVTVKHRIGIDDLDSYEFLCDFIEKVQ
                     PYSNDFIVHARKAWLTGLSPKENREIPPLDYDRVYQLKRDFPHLKISINGGIKTIEEI
                     KQHLQFVDGVMVGREAYQNPSMLGEIDSQIFNENRPLVTAKEAVEKMFPYIERELAKG
                     VYLNHIVRHMLGAFQHCKGARQWRRHLSENATKSGAGVEVVEQALAFIG"
BASE COUNT         1348 a          956 c          898 g         1135 t
ORIGIN      
        1 caaaattttt tgcaaattta accgcttgta ttaaaagtta cccttttgag ttggaacatt
       61 ttatgcaaca agccaaaacc ctatatcaaa aactattcga ctcacacgtt gtgtatgaag
      121 ccgaaggcga aacgccgatt atttatatta accgccactt aattcacgaa gttaccagcc
      181 cgcaagcgtt tgacggcttg cgtgttgccg gtcgccaagt tcgccaagtc agtaaaacct
      241 tcggcacaat ggaccacagt atttcaaccc aagtgcgtga tgtacagcaa cttgaagggc
      301 aagcgaaaat tcaagtatta gaattagaaa aaaactgcga agaaaccggt atcccactgt
      361 ttgacatcac taaaaaacaa caaggtatcg ttcacgtaat ggggccggag caaggcttaa
      421 ctttacccgg tatgaccatt gtatgtggtg actcgcacac cgccactcac ggggcctttg
      481 gtgcgttagc cttcggtatc ggtacttccg aagtggaaca cgttttagcc acccaaacct
      541 taaaacaggc ccgtgcaaaa agtatgaaag tggaagtccg tggcaaagta aacccgggca
      601 ttaccgcaaa agatattgtg cttgcgatca tcggtaaaac aactatggct ggcggtacag
      661 gctatgtagt ggaattttgt ggcgaagcaa ttcgtgatct ttcaatggaa gggcgaatga
      721 cagtgtgtaa tatggcgatt gagtttggtg cgaaagccgg tattgttgcc ccggatgaaa
      781 ccacgttcgc ttacttgaaa gaccgcccaa atgcaccaaa aggtcaagat tgggaagatg
      841 ccattgaata ttggaaaacc ctcaaagccg atgagggtgc agtttacgat gcggaagtga
      901 tcttagaagc aaaagacatt gctccacaag tgacttgggg aaccaacccg gggcaggtga
      961 tcggtattga tgatgtaatt ccaaatccgg aagatatggc cgatcctgtt actcgtgcct
     1021 ctgcggaaaa agccttagct tacatcggct tagaagccca tacagatatg aaagatgtac
     1081 cggttgatca agtctttatt ggttcttgta ctaatgctcg tattgaagat ttgcgtgctg
     1141 cggcagcagt aatgaaaggg cgtaaaaaag ccgacaatgt aaaacgtatt ctcgttgtgc
     1201 caggttcagg tttggtaaaa gagcaagcag aaaaagaggg cttggataaa atcttcattg
     1261 aagccggtgc agaatggcgt aacccgggtt gctcaatgtg tttgggaatg aacgatgacc
     1321 gcttgggcga atgggaacgt tgtgcctcta cgtctaaccg taactttgag ggcagacaag
     1381 gacgtaacgg tcgtacccac ttagtcagcc cggcaatggc ggctgcggcg gcggtgttcg
     1441 gtaaatttat ggatattcgc aaagtgtcat taaacgcata ataggaggaa aaaatggcag
     1501 gtattaaaca acattcaggc ttggtcgttc ctcttgatgc agcgaacgta gacaccgatg
     1561 caatcattcc aaaacaattt ttgcaagcca ttactcgagt cggctttggt aagcaccttt
     1621 tccacgaatg gcgttattta gatgcggaag agactcaaat caaccccgat ttcgtactga
     1681 acttcccaca atatcaaggt gcaaccattt tactcgcccg taaaaattta ggctgtggct
     1741 cttctcgtga gcacgcccct tgggcattgg cagattacgg ttttaaagta atgatcgccc
     1801 caagttttgc ggatattttc tacaataaca gcttaaataa ccatatgttg ccgattaaat
     1861 taaccgaaga ggaagtcgaa gagatcttcc aatgggtgtg ggcaaatgaa ggaaaacaga
     1921 tccacgttga tttagaagcg atgactgtga ctgttggcga gaaagtttat acctttgagt
     1981 tagacgaatt ccgccgccac tgcttattaa acggtttgga taatatcggc ttaaccctac
     2041 aacacgaagc tgcgattgct gcttatgaga aaaacattcc ggcattttta cgctaattaa
     2101 aatacaaaaa aacggctaac atctttaagg cgttagtcgt tttcttttta gccattttct
     2161 ttggcaatta gccttctcgg cactctaacc aaggcacatt atttttgtaa taagttgaag
     2221 taaactctcg tgccactaac tctacaaaac tgccttcaat cggctgataa gaacgataac
     2281 gcacttcaaa ttttgttgct cttgcattca aactgatgtt ggcaagatgg tcgaacggat
     2341 aaacctttcc gccatcaagt tgaaaataga taccaaaatt ctcttgctta cggctttcag
     2401 accaaagtgg aaaataggtt gccaaatatg aactgctgcc tgttgcaaaa ttcttacaat
     2461 aatagctata acgcttaaaa tctttcggat tattcaattc cgcttggctg atattctgtt
     2521 tcaaatatcc aacctgtgct tgttgcttag ttgagtttga ttgtggtgag ggcgtacaag
     2581 caaataagcc aattgccact gttgccatta aagcaatttt gaatttattc ataaaaatcc
     2641 cgataaaata ttaaattaaa tctgatattt gcttttatcg ccaatatcaa aatgcagcgg
     2701 cattcgccat ttttgaatac tcagcacaag aagcaacaag ccacagacaa ggcttaaaat
     2761 ttgcaccaat aaagaccatt ctagtgcaaa agcaatccac aataccgcca ctaaaagcgg
     2821 ttggaaattt aagccgaata ctctaaaaca gaaatattct ttatagctta atccgccgat
     2881 caccagtagc attgccccga acgcaagcaa agggaaatta aacacagcac caagtaagcc
     2941 aatccaaaca gaaaattgga aaatcaggcg aaacgatttg gcataaagat gcaaagaaga
     3001 tgccaacatt gtgccagata ctaataaacc atattgtgcc gtttgcggat aataaggcaa
     3061 taaaagcaac atcaatgtag ctaacacaaa accgctgcgg taaatgatga cggttaaata
     3121 atcccaaaaa tccattggcg attgaatatg tggatctgcc ataactgcct ccttaaacaa
     3181 taaattagcc aataaacgca agggcttgtt ccactacttc tacaccggct cccgacttag
     3241 tggcattttc acttaaatga cgacgccact gcctcgctcc tttgcaatgt tgaaacgcac
     3301 caagcatatg gcgaacgata tgatttaaat agacaccttt ggcaagctca cgttcaatat
     3361 aagggaacat cttttccacc gcttcttttg ccgttacaag cggtcgattt tcattaaaaa
     3421 tttgcgaatc gatctcgcct agcattgacg ggttttgata ggcctcacgc cctaccatta
     3481 ccccatcaac aaactgcaaa tgctgtttaa tttcctcaat ggttttaatg ccgccattga
     3541 tgctaatctt taaatgcgga aaatctcgct tgagctgata aacccgatca taatctaaag
     3601 gcggaatttc acggttttcc ttcgggctta accctgtcag ccacgccttg cgggcgtgta
     3661 cgataaaatc attgctataa ggttgcactt tttcgataaa atcacacaaa aattcatagc
     3721 tgtctaaatc atcaatccca atacgatgtt taaccgtgac cggaatttcc accgcatctt
     3781 gcattgcttt aatacaatcc gccactaaat ctgcttttgc catcaaacaa gccccaaaca
     3841 taccgttttg cactcggtct gacgggcaac cgacatttag attaatctcg gcatagcctc
     3901 gctctgccac caatttggca caatgagcca actgtgccgg gtcgcttcca cccagttgca
     3961 aagccaccgg attctcgctc ggatcatatt ccaacaaatc atatttggcg tgaataatcg
     4021 ccggggcggt gatcatttcg gtataaagca aggcttgacg actaaattgg cgatgaaaat
     4081 agcggcagtg acgggttgtc caatctaaca ttggagcaac cgaaaaacgt ccacggtaaa
     4141 aaggttgaat ttcgttagtc ataaaaatat caaaataaag cggtaaaaaa tcagtaaaaa
     4201 ataaccgctt gtaagcgatt caaaacaagc agccatcata ctcgaaaaag ccagataaac
     4261 atagtttcct caaggcttcc ccatctatct gcgcactttt aaaacaacct taaaacacaa
     4321 tatatagtgt ttcaaca
//
LOCUS       DANLXF010000195         4294 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.195, whole genome shotgun sequence.
ACCESSION   DANLXF010000195 DANLXF010000000
VERSION     DANLXF010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.195"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(47..122)
                     /locus_tag="PXD66_002081"
     tRNA            complement(47..122)
                     /locus_tag="PXD66_002081"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(87..89),aa:Asn,seq:gtt)
     gene            complement(238..987)
                     /gene="moeB"
                     /locus_tag="PXD66_002082"
     CDS             complement(238..987)
                     /gene="moeB"
                     /locus_tag="PXD66_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HDL6320340.1"
                     /translation="MELTDHEMLRYNRQIILKSVDFDGQEKLKASRVLIVGLGGLGCA
                     ASQYLASAGVGHLTLVDFDLVSLSNLQRQILHTDATIGQPKVESAKARLAAINPNIQI
                     ETINQRCSDEQLAKLIANVDLVLDCTDNVTVRNQLNLQCFKQKRPLVSGSAIRFEGQV
                     SVFCYQEQEPCYECLSRLFGDATLSCVEAGVIAPIVGIIGSIQALEAIKVLLNIGRTL
                     SGKLLMIDGLHFSVREMALPKQNNCPVCTSY"
     gene            complement(1086..2315)
                     /gene="moeA"
                     /locus_tag="PXD66_002083"
     CDS             complement(1086..2315)
                     /gene="moeA"
                     /locus_tag="PXD66_002083"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDL6320341.1"
                     /translation="MALLALSDALDNMLARLSTPKLVEQLPLNECANRVLAEDIFSPI
                     NVPNFDNSAMDGYAVRISDLAQNLTLNVAGKAFAGNPFNGEIKAGECVRIMTGALVPK
                     GVDAVVMQEETTLNSDGSVTFNHIPKLGSNIRRIGEDVKQGALVLKKGSLLNVASLPL
                     LASLGIASVPVFVKLKVAILSTGDELTSVGEPLSEGKIYDTNRFAVRLMLEKLNCEIL
                     DFGILPDDPILFEQTFRQAQQQADVLITSGGVSVGEADFTKDVLEKLGEIGFWKIAMK
                     PGKPFAFGKLEQAWFFGLPGNPVSALVTFYQLAQPALAKLSGLSTDKIAKLTQNLTAC
                     SAGKLKKAVGRQDFQRGYYYANEKGELEVRAVGTQGSHIFSAFNESNCFIVLEAERGN
                     VDAGEKVTIQPFNSLMY"
     gene            complement(2337..2954)
                     /locus_tag="PXD66_002084"
     CDS             complement(2337..2954)
                     /locus_tag="PXD66_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006719828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HDL6320342.1"
                     /translation="MFDSLAVQFVVLWAVIDPIGSVPVYLAKTIGLPIEARRKIALNA
                     VLIAAGILIFFLIGGQVLLESMQIPLPAFQAAGGLVLLLFALTMIFGESKPEQEMRIS
                     SNLNELAVYPLAVPSIASPGAMMAIVLLTDNHRFHIGDQIITAGIMLLVLFITYLLLL
                     AANRIQHLIGNAGAAIISRVMGLILAAIAVNNLLIGVRDFFVQIS"
     gene            complement(3022..4233)
                     /locus_tag="PXD66_002085"
     CDS             complement(3022..4233)
                     /locus_tag="PXD66_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL6320343.1"
                     /translation="MISRQMQSLLKRLVAQFPAVLVTGARQVGKSTLLQHIGEGYQYI
                     TFDDPMLLELAKQESRLFFLNHQGKLILDEVQYVPEIFALLKLEIDKKQQNGLFLLSG
                     SQAFELMQNVSETLAGRIAILKLQGLSLREILGVKFDQPFLPTTDYLLAREQELKSPE
                     NIWQLIHKGDMPRLYAQEVDWEIYYRSYVATYIERDVRQLTNIASTTDFTRFMVSIAS
                     RSGELLNYSNIAQEVGVSNETIKRWVAILQTSGIIYLLQPYHNNHLKRAIKTPKVYFL
                     DTGLMAYLTKWLTPETIAQGAKSWQFFETFVVSQIIKSFHNQGIEPPIYFYRDTNQKE
                     IDLIIEYDRTLYPIEIKVTASPTKKMAKSFDLLNSVSSEWKLGQGAIINQYPTKLYLS
                     ENLVALPVSYL"
BASE COUNT         1316 a          996 c          787 g         1195 t
ORIGIN      
        1 tataaaagga gacaaataaa acaagaacaa aagaaaaata aaatcttggc tcctcctgcg
       61 ggactcgaac ctgcgacata tggattaaca gtccaccgtt ctaccgactg aactaaggag
      121 gaattaacgg tgtaaataat aggctaaaac gttttagccg tcaaataaaa aaaataaact
      181 gtcgttcatt ttttacattt cttgacattt tttcgccaat aagtatgaga aaaatactta
      241 ataagaggta caaaccggac agttattttg cttaggtaat gccatttctc tgacagaaaa
      301 atgcaagccg tcaatcatta acaattttcc ggaaagagtt ctgccgatat taagtagcac
      361 tttaattgct tccaacgctt gaatactgcc tatgatgcca acaatcggag caatgacccc
      421 cgcttcaaca caacttaaag tcgcatcacc aaacaacctg cttaaacatt catagcacgg
      481 ttcttgttct tgataacaaa atacactcac ctgcccttca aatcgaattg ccgaaccgga
      541 aacaagcggt cgtttttgct taaaacattg caaatttaac tgattacgca cagtaacgtt
      601 atcggtacaa tccagcacta aatctacatt tgcaatcagc ttggctaact gctcatcgga
      661 acatcgctga ttgatagttt caatctgaat gttcggatta atcgccgcta atcgagcttt
      721 ggcagattcc acctttggct gcccgatagt cgcatcggta tgtaaaattt ggcgttgcag
      781 attagaaagt gagaccagat caaaatccac caaagtcaaa tgcccaacgc cggcagaagc
      841 taagtattgc gatgccgcac agcccaaacc acctaaaccg acaatcaaca ctcggctggc
      901 ttttaacttt tcctgcccgt caaaatccac gctttttagg ataatctggc gattataacg
      961 tagcatttcg tgatcggtaa gttccatttt tacacctaaa gagtaaacga tggcgcaagc
     1021 gtccacgctt gtgcctatat ttttattgct agcacaagcg tggacgcttg cgctatctta
     1081 attcattaat acatcagcga attaaacggc tgaatagtga ctttttcccc agcatcaaca
     1141 ttgcctcgtt ctgcttctag cacaataaaa cagttacttt cattaaaagc gctgaaaata
     1201 tgtgagcctt gcgtgccaac tgcacgaact tctaactcac ctttttcatt ggcatagtaa
     1261 tagccccgtt ggaaatcttg gcgaccgacc gcttttttca attttccggc actgcaagcg
     1321 gttagatttt gggttaattt tgcaatttta tctgtcgata aacccgaaag tttggctaac
     1381 gccggttgcg ctaattggta gaaagtcact aatgcagaga cagggttgcc cggcagccca
     1441 aaaaaccaag cctgttctaa tttaccgaat gcaaaaggct tgcccggctt catcgcaatt
     1501 ttccagaaac cgatttcgcc taatttttcc agtacatctt tggtaaaatc tgcctctccc
     1561 acagatacgc cgccactggt aatcaacaca tcggcttgct gttgggcttg cctgaaagtt
     1621 tgctcaaaca gaattgggtc atcaggcaaa atcccaaaat ctaaaatctc gcagttgagt
     1681 ttttccagca ttaaacgcac cgcaaaacgg ttggtgtcgt aaatttttcc ctcactcaaa
     1741 ggctcgccaa ctgaggttaa ttcatcgccg gttgaaagaa ttgccacttt taattttaca
     1801 aaaacaggca cagaagcaat accgagtgaa gctagcaacg gtaaactggc tacattcaat
     1861 aatgagcctt ttttcaagac caatgccccc tgtttaacat cttccccaat gcggcgaatg
     1921 ttactgccta attttggaat atggttaaag gttacagagc catcggaatt taacgtagtt
     1981 tcctcttgca ttacaacggc atcaacgccc tttggcacta atgctcccgt cataatccgc
     2041 acgcattcgc ctgcctttat ttcgccgtta aacgggttac cggcaaaggc tttaccagcc
     2101 acattcaagg ttaaattttg tgctaaatct gaaatacgaa ccgcgtagcc gtccatcgcc
     2161 gaattatcaa aattcggtac attaatcggg gaaaaaatat cctctgccaa cactcggtta
     2221 gcacactcat ttaacggaag ttgttccacc aatttaggtg tcgataaacg agcaagcata
     2281 ttatctaacg catcactcaa agccaaaagt gccataattt cctacctatt caacgtttag
     2341 ctaatttgta caaagaaatc ccgtacgcca atcaacagat tattcaccgc aatcgcagcc
     2401 agaattaacc ccatcactcg gctgataatc gcagcccctg cattaccaat aaggtgctga
     2461 atacggtttg ccgctaatag caacaaatag gtaataaaga gtaccaaaag cataatgccg
     2521 gcggtgataa tttgatcgcc gatatgaaaa cggtgattgt cggttaataa tacgattgcc
     2581 atcatcgccc cgggagaagc aatcgacggt acagctagcg gataaaccgc caattcattc
     2641 agattactgc tgatccgcat ttcttgctcg ggcttgcttt cgccaaaaat catcgttaaa
     2701 gcaaataaca acagcactaa gccgcctgcc gcttggaatg caggtaacgg aatctgcatt
     2761 gattctaaca gcacttgccc accgataagg aagaaaatca aaataccggc ggcaatcaag
     2821 accgcattta gtgcaatttt acgccgtgct tcaatcggca aaccaatggt ttttgccaaa
     2881 taaacaggaa cagaaccaat cgggtcgatc accgcccata aaaccacaaa ttgtaccgct
     2941 aatgaatcaa acatattctc tcctgttatc ttgataattt gcaaaatttt agcataaaac
     3001 aaccgcttgc acggcttttt cttacagata gctcaccggt aacgctacca aattttcact
     3061 taaataaagt ttggtcggat attggttaat aatcgctccc tgccctaatt tccattcact
     3121 agaaactgag tttagcaagt caaaactttt tgccattttt ttggttgggc ttgccgtgac
     3181 cttaatttct atcggataaa gcgtgcgatc atattcaata attaagtcga tctctttctg
     3241 gttagtatca cggtaaaaat aaatcggcgg ctcaattcct tgattatgaa aggattttat
     3301 aatttggctt accacaaagg tttcaaagaa ctgccagctt tttgcccctt gagcaatagt
     3361 ttcgggggta agccatttgg ttaaatacgc cattaacccg gtgtctagaa aatacacttt
     3421 tggggtctta attgcccgtt tcaaatgatt gttgtggtat ggctggagca aatagataat
     3481 gccggaagtc tgcaaaatgg cgacccaacg tttaatcgtt tcattcgata ctccaacttc
     3541 ttgagcaata ttactgtaat tgagtagctc accactacga gaggcaatag agaccataaa
     3601 acgggtaaaa tccgttgtgc tggcaatatt ggttaattgg cgaacatctc gctcaatgta
     3661 ggtcgctaca taagagcgat aataaatctc ccaatccacc tcttgtgcat ataaacgagg
     3721 catatcgcct ttatggataa gctgccagat gttctccggt gattttaatt cttgctcccg
     3781 agctaacaaa tagtcggttg tcggtaaaaa aggttgatcg aacttcaccc ctaaaatttc
     3841 acgcaacgaa agcccttgta attttaaaat agcaatacga ccggcaagtg tttcactcac
     3901 attctgcatt aattcaaagg cttgcgaccc cgaaagtaaa aacaaaccgt tttgctgttt
     3961 tttatcaatt tctaacttta ataacgcaaa aatttcaggc acatactgta cttcgtctaa
     4021 aataagcttg ccttgatggt ttaaaaaaaa caggcgagat tcttgtttag ccagctccag
     4081 caacattggg tcatcaaaag taatatattg atagccctca ccaatatgtt gtaacaatgt
     4141 cgatttacca acctgtcttg cccctgtcac taaaacggca ggaaattgcg ccacgagtcg
     4201 ttttaacagc gattgcattt gccgagaaat catttttcct ccaaaatagc ctaaatcaag
     4261 tttaacctca tatataatgt tgaattaaac ttaa
//
LOCUS       DANLXF010000196         4256 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.196, whole genome shotgun sequence.
ACCESSION   DANLXF010000196 DANLXF010000000
VERSION     DANLXF010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.196"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            202..2136
                     /gene="iolD"
                     /locus_tag="PXD66_002086"
     CDS             202..2136
                     /gene="iolD"
                     /locus_tag="PXD66_002086"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HDL6320344.1"
                     /translation="MQTTRLTVAQALVQFLDNQYLDVDGKEIKFVHGVAAIFGHGNVL
                     GIGQALAQNAGGLTLYQGRNEQGMAHLAMGFAKQNLRQRIIACTSSVGPGAANMVTAA
                     ATATANRIPLLLLPGDVFATRQPDPVLQQIEQPYDLSISTNDAFRAVSKYWDRVQRPE
                     QLMSACINAFRVLTDPAETGAVTIALPQDVQAESFDFPNSFLQKRVHRLERTPPTTAM
                     IKQAVTLIQTKKKPLIVCGGGVRYSQAGQILQKFAKQFGIPFAETQAGKSAVVSDHAL
                     NVGGVGETGCLAANRLAKEADLVIGIGTRYTDFTTSSKWIFQNPNVQFLNINVARFDA
                     YKLDGVQLVADAKTALEALSQAIPTDYQAQWENEISQAKADFADEIQRLRAVQYHPDF
                     TPEINDNLDHKAVHNEFIQLTGSQLTQTQVLGIVNETIDNNAIIVAAAGSLPGDLQRT
                     WLSKGEETYHLEYGYSCMGYEVSAAVGVKIARPEQEVYALLGDGSYLMAHTELVTSIQ
                     EGKKINVVLFDNMANGCINNLQIGNGMDSFGTEFRFRNGETNQLNGGLVPIDFAKNAE
                     AYGCKTYRVTNEAELRAALDDAKKQTVSTLIDIKVLPKTMAHGYGCWWHVGVAEVSES
                     ERIQQARQRSEEQLAKARKY"
     gene            2263..3159
                     /gene="iolE"
                     /locus_tag="PXD66_002087"
     CDS             2263..3159
                     /gene="iolE"
                     /locus_tag="PXD66_002087"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011817366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HDL6320345.1"
                     /translation="MKAENIQLGIAPIGWTNDDLPELGAENTFEQCVSEMALAGYTGC
                     EVGNKYPRDVAVLKHKLDVRGIQICNAWFSTFFVDGKREETIAEFIKHRDFLHAMGAK
                     VIGCSEQSRSIQGTTKSVFKEKPIFNDEDWKRMAEGYNELAKLAAEKGMKVCLHHHMG
                     TGIQTPEEVDRYMAEVNDDVYLLFDSGHLYYSEGCQKAMLGVLEKYIDRIIHVHLKDV
                     RDEVMAEVKAKDLSFLEGVKKGTFTVPGDGVIDFRPIFDILEKHDYKGWMVVEAEQDP
                     ALANPFEYALKGRKYIRETAGV"
     gene            3161..4171
                     /gene="iolG"
                     /locus_tag="PXD66_002088"
     CDS             3161..4171
                     /gene="iolG"
                     /locus_tag="PXD66_002088"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005537901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HDL6320346.1"
                     /translation="MLKVGIIGAGRIGRVHSESISKYVKGAEIKAISDVKITEELTAW
                     AKSMGIPEVYDDYKKILQDPEIDAVLVCSSTNTHAPISIEAAQAGKHVFCEKPVDADP
                     AKIREVLSAVEKAGVKFQVGFNRRFDHNFKAIKDRVAAGDIGEPHVIRVTSRDPEAPP
                     IEYVKVSGGMFFDMTIHDFDMIRYLSGSEVEEVFAVGTVLVNPEIGKAGDIDTAVITL
                     KLKNGAIGVIDNSRKAAYGYDQRAEVFGSKGAIHITNDTGSTAVFSGENGVVAEKPKY
                     FFLERYMQSFADEISCFVDSVVNDKPTLVNGNDGLQPVLIALAAKKSLEENRPVKLSE
                     VA"
BASE COUNT         1247 a          926 c         1032 g         1051 t
ORIGIN      
        1 tcaaatttat tctatttaaa ttatcatttc tgtgatctag ctctcacttt gaaaaataag
       61 gcaataactt cgcagaaatg agaactggat cacatctttt tttcgatttc cttgctatta
      121 taaatgaaat aaatttttca tttgtaaaaa tcagtagaaa accgaccgct tgcaaaagcg
      181 atgtaagcca gaggaggctt tatgcagact actcgtttaa cggttgcaca ggcgttagtg
      241 caatttttgg ataatcaata tttagacgtg gacggcaaag agattaaatt cgttcacggt
      301 gttgccgcta ttttcggcca cggcaatgtg ctgggaatag gtcaggcgct tgctcaaaat
      361 gccggtggtt taacgctcta ccaaggcaga aatgagcaag gtatggcaca tttggcgatg
      421 ggctttgcta agcaaaatct gcgtcaaaga attattgcct gtacttcctc ggtcgggccg
      481 ggggcggcaa atatggttac ggctgcggca acggcaacag cgaaccgtat tcctcttctt
      541 ttattaccgg gcgatgtgtt tgcgacccgc cagccggatc cggtactgca acaaatcgaa
      601 caaccttacg atttaagcat tagcactaac gatgcgttcc gtgccgtcag taaatattgg
      661 gatagagtgc aacgccctga gcagttaatg agtgcttgta tcaatgcgtt tcgtgtgctg
      721 accgaccctg ctgaaaccgg tgctgtcacc attgctctgc cgcaagatgt acaagcggag
      781 agttttgatt tcccgaacag cttcctgcaa aaacgagtgc atcgcttaga gcgtacaccg
      841 ccgacaacag cgatgattaa gcaagcggta acattaattc aaaccaagaa aaaaccgctg
      901 attgtctgcg gtggcggtgt tcgctactcg caagcgggtc aaattctgca aaaatttgct
      961 aaacagttcg gcattccatt tgctgaaacc caagcgggta aaagtgcggt ggtgtccgat
     1021 cacgcattga atgtaggcgg agtgggagaa accggttgtt tagcggcaaa ccgtctggca
     1081 aaagaagcgg atttagtgat tggcattggt acacgctaca ccgacttcac gacttcttcc
     1141 aaatggattt tccaaaaccc aaacgtgcag tttttgaaca ttaatgtggc acgttttgat
     1201 gcgtacaaat tagacggcgt gcaactggtt gccgatgcaa aaactgcatt agaggcgtta
     1261 agccaagcca ttcctactga ttatcaagct caatgggaga atgaaatttc acaggcgaaa
     1321 gcggattttg ctgatgaaat tcaacgcctg cgcgctgtgc aatatcaccc tgatttcacc
     1381 cctgaaatta acgataatct tgatcataaa gcggttcaca atgaatttat tcagctcacc
     1441 ggttcccagc tcacccaaac ccaagtgctg ggtattgtga atgaaaccat tgataacaat
     1501 gcgattattg ttgccgccgc cggaagtttg ccgggtgatt tacaacgcac ttggttaagt
     1561 aaaggtgaag agacttacca ccttgaatat ggctactcct gtatgggcta tgaagtgagt
     1621 gcggcagtcg gcgtgaaaat tgcccgcccc gaacaagagg tctatgcctt gctgggggac
     1681 ggctcttact taatggcaca caccgaatta gtgacctcaa ttcaagaggg caaaaaaatc
     1741 aacgtggtgc tgttcgacaa tatggcaaac ggctgtatca acaacctgca aatcggcaac
     1801 ggtatggaca gtttcggtac ggaattccgc ttccgcaatg gcgaaactaa ccaactcaac
     1861 ggcggtttag tgccgattga ttttgccaaa aacgccgaag catacggctg caaaacctat
     1921 cgggtaacta acgaagccga attgcgtgcc gcccttgatg atgcgaaaaa acagaccgtt
     1981 tctaccttaa tcgatattaa agtgttgccg aaaacaatgg ctcacggcta tggctgctgg
     2041 tggcacgttg gtgtcgcgga agtgtcagag agcgagcgta ttcaacaagc ccgccaacgc
     2101 tccgaagagc agttagctaa ggctcggaag tattagttaa aaaaacaact ccccactttg
     2161 agctacgagg ggagaatatc aagcggtcaa atttgcaaaa tttttgataa aaataaccgc
     2221 ttgcatccaa atttatctct ataaatcaaa cgaggtttct ctatgaaagc tgaaaacatt
     2281 caattaggca ttgccccaat cggttggacg aacgatgacc tgccggaact gggagcggaa
     2341 aacactttcg agcagtgtgt gagtgagatg gcacttgccg gctacacggg ctgtgaagtg
     2401 ggtaataaat atccgcgtga tgttgccgtg ttaaaacaca aactggacgt gcgtggcatt
     2461 caaatctgta atgcgtggtt cagcaccttc tttgtggacg gtaaacggga agagaccatt
     2521 gccgagttca tcaaacatcg tgatttcttg catgcaatgg gggcaaaagt gatcggctgt
     2581 tccgaacaga gccgtagtat tcaaggcaca accaaatctg tgttcaaaga aaaaccgatt
     2641 ttcaacgatg aagattggaa acgtatggca gaaggctata acgaattggc gaaacttgcc
     2701 gccgaaaaag gaatgaaagt ctgcctgcat caccatatgg gaaccggtat tcaaacaccc
     2761 gaagaggtcg atcgctatat ggcagaagtg aatgacgatg tctatctgtt gttcgattca
     2821 gggcatttgt actattccga aggctgccaa aaagcgatgc tcggcgtact ggaaaaatat
     2881 atcgaccgta ttatccacgt tcacttaaaa gatgtgcgtg atgaagtcat ggcggaagta
     2941 aaagcaaaag atttaagctt tttagaaggc gtgaaaaaag gcacctttac tgtaccgggc
     3001 gatggtgtca ttgatttccg tccgattttc gatattttag aaaaacacga ctacaaaggc
     3061 tggatggttg tagaagccga acaagatccg gcacttgcca acccgtttga atacgccctc
     3121 aaaggcagaa aatatatccg tgaaaccgca ggagtttaat atgttaaaag taggtattat
     3181 tggtgcaggc cgtattggac gtgtgcattc tgaaagtatt agcaaatatg tcaaaggtgc
     3241 tgaaatcaaa gcaatctctg atgtaaaaat taccgaagaa ttgaccgctt gggcaaaatc
     3301 gatgggtatt ccggaagttt atgatgacta taaaaaaatc ctgcaagatc cggaaattga
     3361 tgctgtattg gtctgctctt ccaccaacac tcacgctccg atttcgattg aggcagcaca
     3421 ggctggcaaa cacgttttct gtgaaaaacc ggtagatgca gacccagcta aaatccgtga
     3481 agtattgagt gcagtcgaaa aagccggtgt aaaattccaa gtgggcttta accgccgctt
     3541 cgaccacaac ttcaaggcga ttaaagaccg tgtggcggca ggcgacatcg gcgaaccgca
     3601 cgttatccga gtaacctctc gtgatccgga agccccgcca atcgaatatg tgaaagtctc
     3661 cggcggaatg ttctttgata tgaccattca cgacttcgat atgattcgtt atttatccgg
     3721 cagcgaagtg gaagaagtgt ttgcagtcgg caccgtatta gtaaaccctg aaatcggcaa
     3781 agcgggcgat attgatactg ccgttatcac gttgaaactg aaaaacggag caatcggcgt
     3841 aatcgacaac agtcgcaaag ctgcctacgg ctacgaccaa cgtgcggaag tgttcggctc
     3901 gaaaggggca atccatatca ctaatgatac cggctcaact gccgtattct caggtgaaaa
     3961 cggcgtggtg gcggaaaaac caaaatactt cttcttagaa cgttatatgc aatctttcgc
     4021 cgatgaaatc agctgtttcg tagattctgt ggtaaacgac aaaccaacgt tagtaaacgg
     4081 caatgacggc ttacaaccgg tacttattgc cttagctgcg aaaaaatcac tggaagaaaa
     4141 ccgaccagtg aaattgagtg aagtggcgta attgatcata aatacaagcg gtcggtttta
     4201 ctcgctacac tcgcacttcg tgccatcggc atagccgatg ttcaaacgca agcatt
//
LOCUS       DANLXF010000197         4236 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.197, whole genome shotgun sequence.
ACCESSION   DANLXF010000197 DANLXF010000000
VERSION     DANLXF010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.197"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..1174
                     /locus_tag="PXD66_002089"
     CDS             <1..1174
                     /locus_tag="PXD66_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDL6320347.1"
                     /translation="SSSLKFAILDPKTGDEKLSGLAEAFHLEDARIKWKLNGEKGNAD
                     LGAGAAHSEALTFIAEKLLSDELKAGIGAIGHRIVHGGEKFTSSVVITDEVIKGIEDA
                     TQFAPLHNPAHLIGIKEAFRIFPELKDKNVAVFDTAFHQTMPEHAFLYALPYTFYKEN
                     GIRRYGFHGTSHFYITSQVAELAGKSVEQTNAIICHLGNGGSVSVVRNGKCIDTSMGL
                     TPLEGLVMGTRSGDLDPAIISFLYNNLGMSMDEIDTTLVKKSGLLGLTGVTSDCRYAE
                     DNYNDVSKPEAKRALDVYSYRLAKYIGSYMAILGDDHLDAIAFTGGIGENSGLVRELT
                     LNHLKLFGIKLDQERNLAARFGKDGVITADESTFKAVVIPTNEELVIAQDTAKLAL"
     gene            1227..3362
                     /gene="pta"
                     /locus_tag="PXD66_002090"
     CDS             1227..3362
                     /gene="pta"
                     /locus_tag="PXD66_002090"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005542370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDL6320348.1"
                     /translation="MSRTIILIPISTGVGLTSVSLGLVHALEQKGSKVGFLKPISQPI
                     SGEDTLDRSTTIIRAEQTTEVGEPFMLSEAETLIGQNQTDVLLEKVVERHQKLSKNNE
                     IVIVEGLIPTRKNSYANSINYEMAQALDAEIVLVAAPASDTPAQLKERVEAAASQFGG
                     RNNPNLLGVIINKFNAPVDDSGRTRPDLTEIFDSFQHSNNNVSEVEHLFAKSPIKLLG
                     CISWKAELIATRAIDVAKHLGASIINEGEIKARRIRGVTFCARNLPNMVDHFKAGSLL
                     VTSADRPEVLVAASLAVMNGIEIGAILLTGGYKIDSSIAKLCQQAFETGVPVFRIEGN
                     TWQTALSLQSFSLEVPVDDKERIAAIKEYMASKFNVGFIDEVSKAATRARRLSPAAFR
                     YQLTEYARQAKKRIVLPEGDEPRTVKAAALCAERGIAECVLLANPTDVQRVAEAQGVV
                     LGKGVTIIDPASIRENYVDRLVELRKAKGMTEVVAREQLLDTVVLGTMMLEANEVDGL
                     VSGAVHTTANTIRPPMQIIKTAPGSSIVSSIFFMLLPDQVLVYGDCAVNPDPTAEQLA
                     EIAIQSAESAKAFGIDPRVAMISYSTGTSGSGADVEKVKEATRIAQEKRPDLIIDGPL
                     QYDAAIMEDVARSKAPNSPVAGKATVFVFPDLNTGNTTYKAVQRSADLVSIGPMLQGM
                     RKPVNDLSRGALVDDIVYTIALTAIQATQ"
     gene            3476..4156
                     /gene="can"
                     /locus_tag="PXD66_002091"
     CDS             3476..4156
                     /gene="can"
                     /locus_tag="PXD66_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HDL6320349.1"
                     /translation="MEKIERLFANNHAWATKMKDEQSDYFKELAEHQKPTYLWIGCSD
                     SRVPAEKLTGLGPGELFVHRNVANMVIHTDLNCLSVVQYAVDVLEIEHIIICGHTNCG
                     GINAAIAQKELGLINNWLLHIRDIWFKHSHLLGKLPYEERSNVLTRLNVAEQVYNLGQ
                     SSIIKTAWEQGKSLSLHGWVYDVTDGFLIDQGVMATSRETLEITYRNAIAKITTEANE
                     KIVLNDEN"
BASE COUNT         1317 a          783 c          867 g         1269 t
ORIGIN      
        1 tagttcatct cttaaatttg ctattttaga tcctaaaaca ggtgatgaaa aattatctgg
       61 acttgctgaa gcttttcatt tagaagatgc acgtatcaag tggaagttaa acggggagaa
      121 aggtaatgct gatttaggtg caggtgccgc acatagtgaa gcacttacct ttattgctga
      181 aaaattgtta tctgacgaat taaaagcagg tatcggcgcg attggtcatc gtattgttca
      241 tggcggtgaa aaattcacct catcagttgt aattaccgat gaagtgatta aaggtattga
      301 agatgcaacg caatttgcac cattacacaa ccctgcgcat ttaattggta ttaaagaagc
      361 attccgcatt ttccctgaat taaaagacaa aaacgtagct gtatttgaca ctgcattcca
      421 tcagacaatg ccggaacacg cattcttata tgcacttcca tatacattct ataaagaaaa
      481 tggtattcgt cgctacggtt tccatggcac aagccacttc tatatcacct cacaagttgc
      541 agaattagca ggtaagtctg ttgagcaaac caatgccatt atttgccact taggtaacgg
      601 tggttctgtt tctgttgtac gcaatggtaa atgtattgat acctcaatgg gtttaactcc
      661 attagaaggt ttagtaatgg gtactcgctc tggtgactta gaccctgcga ttatctcttt
      721 cttatacaat aacttaggta tgtcaatgga cgaaattgat actactctag taaaaaaatc
      781 tggtctttta ggtttaactg gcgtaactag tgactgtcgt tatgcggaag ataattacaa
      841 tgacgtatca aaaccggaag caaaacgtgc tttagatgta tatagctatc gtttagctaa
      901 atatattggt tcatatatgg caattttagg cgatgaccac ttagatgcta tcgcatttac
      961 aggtggtatc ggtgaaaatt caggcttagt acgtgaatta accttaaatc acttaaaatt
     1021 atttggcatt aagttagatc aagagcgtaa ccttgctgcc cgttttggta aagatggagt
     1081 cattacagcg gacgagtcaa catttaaagc tgttgttatc ccaactaatg aagaactcgt
     1141 gattgcacaa gataccgcta aattagcatt ataatcatag ggggcagata ttctgccctt
     1201 tcactttctt ttaatttgga aatattatgt ctagaacgat cattcttatc cctatttcaa
     1261 ctggtgttgg tttaaccagt gttagcttag gtttagtaca tgctttggag caaaaaggtt
     1321 ctaaagtagg atttttaaaa ccgatttctc aacctatcag cggtgaagat acgttagacc
     1381 gctcaacaac gattatcaga gcagaacaaa caacagaggt tggtgagccg tttatgttaa
     1441 gcgaagctga aacattaatc ggtcaaaacc aaacggatgt attgttagaa aaagtggtgg
     1501 aacgccacca aaaattatca aaaaataatg aaattgtcat tgttgaaggt ttaattccaa
     1561 cgcgtaaaaa ctcatacgct aacagcatta actatgaaat ggctcaagcc cttgatgctg
     1621 aaattgtatt agttgcagca ccggcctcag atacaccagc tcaattaaaa gagcgagttg
     1681 aagctgccgc ttcacaattt ggtggacgta acaacccaaa tttactgggc gttattatca
     1741 ataaattcaa tgcaccagta gatgattcag gtagaacccg tccagatttg actgaaattt
     1801 ttgattcatt tcaacactca aataataatg tttcagaagt cgagcatctt tttgctaaaa
     1861 gtccaattaa gctgttaggc tgtattagct ggaaagcaga acttattgct acacgagcaa
     1921 ttgatgttgc aaaacattta ggcgcttcaa ttattaatga aggcgaaata aaggctcgtc
     1981 ggattcgcgg tgtgacattc tgtgcacgta acttaccaaa tatggtcgat cattttaaag
     2041 ccggtagttt attagttacc tctgcggatc gtcctgaagt gttagttgct gcatcgttgg
     2101 cggtaatgaa tggcatcgaa attggtgcta ttttactaac cggtggttat aaaattgata
     2161 gctcaattgc aaaactttgc caacaagcat ttgaaaccgg tgtgcctgta ttccgtattg
     2221 aaggtaacac ttggcaaacc gcattaagcc tacaaagctt cagcttagaa gtgcctgtag
     2281 atgataaaga gcgtattgct gcaattaaag aatatatggc aagcaaattt aatgtaggtt
     2341 tcattgatga ggtttctaaa gccgctactc gtgcaagacg tttatcacct gcagcattcc
     2401 gttaccaatt aaccgaatat gcccgtcaag cgaaaaaacg cattgtttta cctgaaggag
     2461 atgagcctcg tactgtgaaa gcggctgcat tatgtgctga gcgtggcatt gcagaatgtg
     2521 tgttattagc taacccaaca gacgtacaac gtgttgctga agcacaaggc gtggtattag
     2581 gtaaaggcgt aacgattatt gaccctgcga gcattcgtga aaattatgtt gatcgcttag
     2641 ttgaattgcg taaagcaaaa ggaatgactg aagttgttgc tcgtgagcaa cttttagata
     2701 cagttgtatt gggtacaatg atgcttgaag ctaatgaagt ggacggttta gtctcaggcg
     2761 cagttcacac aaccgctaac actattcgcc caccaatgca gatcattaaa actgcaccgg
     2821 gaagctctat tgtatcttct atcttcttta tgctactacc tgatcaagta ttggtatatg
     2881 gtgactgtgc ggtaaaccct gacccaactg cagaacagct agcggaaatt gcgattcaat
     2941 cagcagaatc tgccaaagcc tttggtattg accctcgtgt tgcaatgatc tcctactcta
     3001 ccggtacatc aggttcaggt gcggatgtag aaaaagtgaa agaagcaact cgtattgccc
     3061 aagaaaaacg tccagattta atcattgatg gcccattaca atatgatgcg gcgattatgg
     3121 aagatgttgc ccgctcaaaa gcaccaaatt cacctgtagc aggtaaagca actgtatttg
     3181 tcttcccgga tttaaatact ggtaatacca cctataaagc ggtacaacgt tcggcagatt
     3241 tagtctctat cggcccaatg ttacaaggta tgcgtaaacc tgtaaatgac ttgtcccgtg
     3301 gggctttagt tgacgacatc gtttatacta ttgcgttaac tgcgattcaa gcaacgcaat
     3361 aattccctcc caaatgaaac caaatgccct taaaactaag ggcattttta ttttttacct
     3421 acccgacttt tcaatttaat catataatca agattagtgt taaaaaggag aatttatgga
     3481 aaaaattgaa cgcttatttg ccaataacca cgcctgggca actaagatga aagatgaaca
     3541 atcggattat tttaaagaat tagcggaaca tcaaaagcca acctatttat ggattggctg
     3601 ttccgatagt cgtgtgcctg ctgaaaaact cacagggtta ggccccggag aattatttgt
     3661 tcatcgaaat gtagcaaata tggtgattca cactgatctt aattgtctgt cggttgttca
     3721 atatgcggta gatgtattag aaattgagca tattattatc tgtggtcaca ctaattgtgg
     3781 tggtatcaat gcggcaattg cacaaaaaga acttggttta attaataact ggttgttaca
     3841 tattcgtgat atctggttta aacacagcca tttactaggc aaacttccct atgaagagcg
     3901 atcaaatgtc ttgacccgat taaatgttgc agaacaagtt tataatttag gtcagtcctc
     3961 tattattaaa accgcttggg aacagggtaa atccctctcg ctacacggtt gggtatacga
     4021 tgttacagat ggtttcttga ttgaccaagg tgttatggct actagccgag aaacattaga
     4081 aattacctat cgtaatgcaa tagctaaaat aacaaccgaa gcaaacgaaa aaatagtttt
     4141 aaacgatgaa aattagccat tgtaagcggt taaatttatc gattttttta caatcttttt
     4201 ataaaaaacc attgtttgat ggttttttat tttcct
//
LOCUS       DANLXF010000198         4227 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.198, whole genome shotgun sequence.
ACCESSION   DANLXF010000198 DANLXF010000000
VERSION     DANLXF010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.198"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..1451
                     /locus_tag="PXD66_002092"
     CDS             <1..1451
                     /locus_tag="PXD66_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320350.1"
                     /translation="TFKLTLAALAVSIVLSGCKSSQGPSIVADSPPPKEITATDGNDK
                     EKTDNKEKKSAQPNKSDPQPDKPANAKDPVKPDAETNKPSKKDKEYTPPATEKIDNSS
                     VQPDKMVPNPIENKENDSNKPNPKESDSDNNDKVTEGNKPNAPTETVNKDKEAEKSKK
                     ENKEDSSNNTPNINFDGAKPIDTDSKGSPYRVINLNPRKSVVNASAMQKVDFDSFTDE
                     SVENSPFVSSDTEKEYNNTLVFGLGAGDKDSYFKNLNQKGDKHLGIHSGVYEDANLEG
                     NTKKINYLYVNQPYSSYGALFTDQKDSHLFNVRLTAGRDDVKENGDSYAEYGVFTQSG
                     KEAKWNDNLIGDATYKGQAIARVIRQVNGEQVAGIPQFDGDVTLNLHLSKEWNESKLN
                     GKIDSQSVGVITLKESEIANPTALRSNIGFGEEVIVENKENLVGDYAVDFTGPQLEEA
                     VGVVELENEAETFSENEVIKYNAVFGVSKAAK"
     gene            1515..2927
                     /locus_tag="PXD66_002093"
     CDS             1515..2927
                     /locus_tag="PXD66_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL6320351.1"
                     /translation="MKKIALLALFPMMLFARDPNTTQAYIEHTAQPTTTPQRVKIEKS
                     VPIFNETELRANQLLTEKLLIQAIYSRRLANMEKLTAIYRDFTHIDPVLLRYAQGKIA
                     LLKGDYAQAIQHFQTILAKQPKLNTVRIELAIALLYDQQLNQAQAQFEKAKSAEHLPP
                     KVHALIEQYQQHLAKKQEWKFSFSANYVRDGNINRTSKQPNIEQTGYVKQPNLLPQTA
                     HGVHYAFGLQRDFNLFDSHYVSFDNQTQGELYWDNHDYDEITNRTLFGYAYKKSDQTT
                     RLLPFYEKRWKHHRSERWTNGIRLEHAQWFSPHWQLATALEYGKKRYFDLPIYNGSYK
                     LASATLLWLRNPQQFFFVGTDFNQDKTQLKQYSSDTKGIRFGWQREWQALELSSRLTL
                     GYTDRRYKDTAILGGKLNLGRIRHDRVQSANLTLWKRDWHWLGLMPKLQFSYAKHRSN
                     LPSLYAYTDKNINLIIESRF"
     gene            complement(3177..3500)
                     /locus_tag="PXD66_002094"
     CDS             complement(3177..3500)
                     /locus_tag="PXD66_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin"
                     /protein_id="HDL6320352.1"
                     /translation="MQKAYQIYTHSEREKVTEAKLELGKVETRLRNEGKTKEEIAQHP
                     EYLQLKRDLNDKQQTFDNAYGTGSKTKRAVDAVTAALQGLAAKDSGQAAVGLASPYLN
                     EQIKK"
     gene            complement(3469..3996)
                     /locus_tag="PXD66_002095"
     CDS             complement(3469..3996)
                     /locus_tag="PXD66_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HDL6320353.1"
                     /translation="MRDKNKERQQTLERYQELYDQDPELRVIAANSEGDLDLLQETFF
                     VKGELTITDSTPKEKWNWLHMCNLNSYSPSPLSVVEFYIKHGVDVNAQDCYGMTPLHY
                     AMRAKNVDAAIALLNAGADPNIENKDQVIPLSMIGAVPKEIELIEHDISEVKETQELV
                     KGIGEIAEGLSDLHA"
     gene            complement(3998..4210)
                     /locus_tag="PXD66_002096"
     CDS             complement(3998..4210)
                     /locus_tag="PXD66_002096"
                     /inference="COORDINATES: protein motif:HMM:NF025767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH/ENDO VII family nuclease"
                     /protein_id="HDL6320354.1"
                     /translation="MYCWEHRRLSLAAKELNWSQKQFNDYVNARPENFRLENISENRS
                     HKNEMPGKDNIKSIIDDMKKFQQIGK"
BASE COUNT         1353 a          895 c          772 g         1207 t
ORIGIN      
        1 ctacattcaa acttactctt gcagctttag ctgtttctat tgttttaagt ggctgtaaat
       61 ctagccaagg tccatctatt gttgcagaca gccctccacc taaggaaata acagctactg
      121 atggcaacga taaagagaaa accgataaca aagagaaaaa gtctgcgcaa ccaaataaat
      181 ccgatcctca accggataaa cctgctaacg caaaagaccc agtaaaacct gatgcggaga
      241 caaataaacc aagtaaaaaa gataaagaat atacaccacc tgctactgaa aaaatcgata
      301 actcatcagt acagccggat aaaatggtgc ctaatcctat cgagaataag gaaaacgaca
      361 gtaataaacc taaccccaaa gaaagcgata gcgataataa tgacaaagtc actgaaggca
      421 ataaaccaaa tgccccaaca gaaacagtaa ataaagataa agaagctgag aaaagtaaaa
      481 aggagaataa agaggactca agcaataata ctcctaacat taattttgac ggggcaaagc
      541 ctatagatac agatagtaaa ggtagcccgt accgagtaat aaacctaaat ccgagaaaaa
      601 gcgtagtaaa tgcaagtgct atgcaaaaag tagatttcga ctcttttacg gatgaatctg
      661 ttgaaaattc accatttgtc agtagcgata ctgaaaaaga atacaataat acacttgttt
      721 ttgggcttgg tgccggagat aaagattctt attttaagaa cttaaatcaa aaaggggata
      781 aacatttagg tattcatagt ggcgtgtatg aagatgcgaa tttagaaggt aatactaaaa
      841 aaattaacta tctctatgta aatcaacctt attcgagcta tggtgcttta tttacggacc
      901 aaaaagacag ccatttattc aacgttcgtc tgaccgctgg tagagatgat gtaaaagaaa
      961 atggagatag ttatgccgaa tatggtgtat tcacacaatc aggaaaagaa gctaaatgga
     1021 atgataacct gattggtgat gccacttaca aaggtcaagc tattgctcgc gttattcgtc
     1081 aggtaaatgg ggaacaagtc gcaggtattc ctcaatttga tggtgatgtt acattaaatt
     1141 tacacctttc caaagaatgg aatgaaagta aactaaacgg caaaattgat agccaaagtg
     1201 ttggggtaat tacacttaaa gaaagtgaaa tagctaaccc taccgcactc cgctcaaata
     1261 ttggttttgg agaagaagtc atagttgaga ataaagagaa tcttgtgggt gattacgctg
     1321 tcgattttac cgggcctcag cttgaagagg cggtaggcgt tgttgaatta gaaaacgaag
     1381 ccgagacatt ctctgaaaat gaagtcatta aatacaatgc ggtatttggc gtaagcaaag
     1441 cggcgaaata gtattttttg tatcttacaa agggctgtta agccctttta tttttattat
     1501 tttaaggata aaaaatgaaa aaaattgcct tactggcact tttcccaatg atgttatttg
     1561 cccgagatcc taatactaca caggcttata ttgagcatac tgcacagcca actacaaccc
     1621 cccaaagggt caagatcgaa aaatcagtgc ctatttttaa cgaaactgaa ttacgtgcta
     1681 atcaattatt gaccgaaaag ctacttatcc aagctattta tagtcgtagg cttgctaaca
     1741 tggaaaaatt aactgcgatt tatcgggatt ttacccacat tgacccggtt ttactgcgtt
     1801 atgcacaagg taaaattgcc ctactgaaag gcgattacgc acaggcaatt cagcacttcc
     1861 aaaccattct tgccaaacaa cccaaactaa acactgtaag aattgagctg gcgattgctc
     1921 tattgtacga tcagcaactt aatcaagccc aagcacaatt tgaaaaagcc aaaagtgccg
     1981 aacatctacc gcctaaagtt cacgcattaa ttgagcaata tcagcaacat ctggcaaaaa
     2041 aacaggaatg gaaatttagt ttttcagcca attatgtgcg agacggcaac attaaccgaa
     2101 cctcgaaaca gccgaacatt gagcaaaccg gctatgtaaa acaacctaat ttgttgcccc
     2161 aaaccgcaca tggtgttcac tatgcgtttg gattacagag agatttcaac ctgttcgact
     2221 cacactatgt gagttttgac aaccaaaccc aaggtgaact ttattgggat aaccacgatt
     2281 atgatgaaat taccaatcgc accttgtttg gatacgctta caaaaaatcc gaccaaacca
     2341 cccgcttgtt accgttttat gaaaaacgct ggaaacatca ccgttcggaa cgctggacaa
     2401 acggaattcg acttgaacac gcacaatggt tttcaccgca ttggcaactc gccaccgcat
     2461 tggaatacgg caaaaaacgt tattttgatt tgccgattta taacggcagc tacaaacttg
     2521 cctctgccac attgctttgg ttacgcaatc cacaacaatt tttctttgtc ggcactgatt
     2581 tcaatcaaga caaaactcag ttaaaacaat atagctctga tactaaaggc atacgctttg
     2641 gctggcaacg agaatggcaa gccctcgagc tttccagtcg cctcacgctt ggctatactg
     2701 acagacgtta caaagacacc gctatattgg gaggaaaact taatcttggc agaatccgcc
     2761 atgaccgtgt gcaaagtgct aacctcacgc tttggaaacg agattggcac tggctcggcc
     2821 tgatgccgaa gctgcaattt agttacgcca aacaccgtag caacctgccg agcctctatg
     2881 cctacaccga taaaaacatc aatttaatta ttgaaagtcg gttctagctc cataaaaaca
     2941 acaagcggtt aaaattccta gaaattttgc aaattgcaaa aaatgtagaa aatttaaccg
     3001 cttgtatcta tactgcagct tcttttcctt cagtataaat taatatacac cgttgcaaca
     3061 ataatggctg ctccctgtca tcaccttgaa ctgaaaattg ctcaaggtgg atatgactat
     3121 cgcagccgct tccaccgcac ccagtaatgt atgggcaagc aggttggctt gggtatctac
     3181 tttttaattt gttcgttgag atatggcgaa gctaagccta ctgctgcttg tcctgaatct
     3241 ttcgccgcta aaccttgtaa ggctgccgtc accgcatcca ccgcacgctt ggttttgctg
     3301 cccgtgccgt aggcgttatc aaaagtctgc tgtttatcgt ttaaatcccg ttttaattgg
     3361 agatattcag gatgttgagc gatttcttct tttgtttttc cctcatttct caaacgggtt
     3421 tccactttgc ccaattccag cttcgcttcc gtcacttttt cacgctcact atgcgtgtaa
     3481 atctgataag ccttctgcaa tctcgcctat gcccttcacc agctcctgtg tttctttcac
     3541 ctcactaata tcgtgttcta ttaactctat ctctttagga actgctccaa tcatagacaa
     3601 tggaataact tgatctttat tttcaatatt cggatctgcc cccgcattta ataatgctat
     3661 cgctgcatca acattctttg ccctcattgc ataatgtaaa ggggtcattc cgtagcaatc
     3721 ttgggcatta acatctaccc catgtttaat gtaaaattcg actacagaaa gaggagatgg
     3781 tgaataacta tttaagttac acatatgtag ccagttccat ttttcttttg gtgtgctatc
     3841 cgtaatagtc agttcacctt ttacgaaaaa tgtttcttgt aaaagatcga gatcgccttc
     3901 tgagtttgct gcaataactc ttaattcagg atcttgatca tataattctt gataacgttc
     3961 taatgtctgc tgacgttctt tattcttatc tctcatttta ctttcctatt tgttggaatt
     4021 ttttcatgtc atcgattatt gatttaatat tatctttccc cggcatctca ttcttatgac
     4081 tccgattttc agatatattt tctaatctga aattctccgg tctcgcatta acataatcat
     4141 taaattgttt ttgactccaa tttaattctt ttgccgctaa tgataatcgg cgatgttccc
     4201 agcaatatat attctatgca tgcacta
//
LOCUS       DANLXF010000199         4204 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.199, whole genome shotgun sequence.
ACCESSION   DANLXF010000199 DANLXF010000000
VERSION     DANLXF010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.199"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            2..1870
                     /locus_tag="PXD66_002097"
     CDS             2..1870
                     /locus_tag="PXD66_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDL6320355.1"
                     /translation="MSGLVSPELIKTVALLATSVTIVPLFKRLGLGSVLGYLVAGCLI
                     GPSGIGLFQDPAAVVHMAELGVVMFLFIIGLEMHPELLWAMRKAIFGRGFLQVAICGT
                     LLTLAGIYLLGFSKEVAFIAGMGFTLSSTAIVMQVLEDKGISSTPKGQRIVATLLFED
                     LAIVPLLASVAFLAPNQVDAESSTNWSAIGLSLVAVLILVASGKWLMNPIFRVISKAK
                     VREMMTAAALLVVLGSALLMEISGLSMAMGAFLAGVMLSESSFRHQLEADIEPFRGLL
                     LGLFFMGVGMSLDLSLVLNNWLWLLVIVAVYVIGKGLGIFIAASITRLTLNESIVRTT
                     IMAHGGEFAFVLFSAAATAGILTPDTHATLTAAVIVSMLLSPLLILIKQGVRKKKAKN
                     ESTNMTGVELPEDLEANVLVIGFGRFNQIVCQALLAKGLSVSVIDSNTDHIRAAARFG
                     FKLYYGDGARLDVLRASGIANANCVIVGVGNPERTEKIVELIKSEYPLVPVFARTYDR
                     QSAVDLVKKHHVDYHIRETFESALALSQAAIERLGSSEEEAKQIISYVRRLDAERFNE
                     ELLHGFSAEVIRKYWVPTPWVNPQHEGSALNEETADILEELGETVEVIKVDITDKN"
     gene            complement(2030..2926)
                     /gene="malM"
                     /locus_tag="PXD66_002098"
     CDS             complement(2030..2926)
                     /gene="malM"
                     /locus_tag="PXD66_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HDL6320356.1"
                     /translation="MKKTLLSTAIFFAHLLVVSPSIANGSAGKSIHINSAELAQIQWQ
                     DVPFSQTVKTDLSEQHKQAFTTAFAGIASPVAAYRIPANQGTLEIEIESPVMDKNVFV
                     PTAVVLDSQFNVASTYPSSEFKLLEERGLKGSRLGAELNLTPAMNQDYIYLLIYTTQQ
                     DLAKTTMMPHPAKTYAKATGKQPPAINDIEVAHSLKGQIHINVTGSNGTKFIGLPTEI
                     FSSNKAATPAAKTAIPLPIKENPVAVKTAVDKDTEAYFNQAVMKAIKAKDINKAMNLV
                     NEAEKLGLTSPRQIFLKNVSSN"
     gene            complement(3007..>4204)
                     /locus_tag="PXD66_002099"
     CDS             complement(3007..>4204)
                     /locus_tag="PXD66_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="maltoporin"
                     /protein_id="HDL6320357.1"
                     /translation="IGWTSGGGEQSAFTVNGGGSKYRLGNESDTYAELKLGQELFKSG
                     EKSIYFDTNLAYGGTLHNNDWTPTSPALRELNVQFKNFADSLPGATLWAGKRFYQRHD
                     VHMNDFYYWDISGPGAGVENIDLGFGKLSLAVTRDTERGGANTFGYTTVYKLDPSTGK
                     IKLDKQRNKADVYNDIFDVRLAGIELWKDGSLELGFDYGNAHAKKGAALEKGATKNGY
                     MVTAEYTQGNFFGGFNKFTAQYATDSMTSWNNGHAQGSKADNKGNMLRLINQGVVQAS
                     DKVEVMYALIYEKTKLDNKQGKTWYSAGIRPMYKWNDTMSTLLEVGYDRIKDQATGKK
                     NDLVKYTVAQQWQAGNSIWARPAIRVFGTYARWNDKFNTKARTDAGYKAKDGEFITGV
                     QFEAWW"
BASE COUNT         1138 a          870 c          857 g         1339 t
ORIGIN      
        1 tatgtcagga ttagttagtc cggaattaat taaaacagtc gcattacttg caaccagcgt
       61 cactattgta ccgctattta aacgccttgg tttaggaagc gtattgggtt atttagtcgc
      121 aggatgttta atcgggccat cgggcattgg attgttccaa gaccctgccg ctgttgtgca
      181 tatggcagaa cttggcgtgg ttatgttttt gtttattatt ggtttggaaa tgcacccgga
      241 actgctttgg gcgatgcgta aagccatctt cgggcgaggt ttcttacaag ttgcgatttg
      301 cggtacttta ttaaccttag ccggcattta cttattaggt ttttccaaag aagtggcatt
      361 tattgccggt atgggtttta cgctatcttc tactgccatt gtaatgcaag tgctggaaga
      421 taaagggata agttccacgc caaaagggca acgtattgtt gcaactttac tatttgaaga
      481 tttagcgatt gtgccattat tagcctcggt tgcctttctt gctcctaacc aagtagatgc
      541 tgaatccagt actaattggt ctgctattgg tcttagccta gttgccgttc ttattttggt
      601 tgcgagtgga aaatggctga tgaaccctat tttccgtgtt atttctaaag ccaaagtacg
      661 agaaatgatg actgccgctg ccttacttgt ggtattaggc tccgctcttt taatggaaat
      721 cagtgggctt tcaatggcaa tgggggcatt ccttgccgga gtaatgcttt cggagtcttc
      781 tttccgccat caactagaag ctgatattga acccttccgt ggtttacttt taggcttatt
      841 ctttatgggc gtaggaatgt cattagacct cagcctcgtc ctcaataatt ggctatggtt
      901 attggtgatt gtagcagttt atgttatcgg taaaggcctt ggaatcttca ttgctgcctc
      961 tatcacccga ttaacactga atgaatctat tgtgcgaact accattatgg ctcacggtgg
     1021 tgaatttgcc tttgtgctat tctcagccgc cgcaaccgct gggattttaa ccccagatac
     1081 tcacgctacc ttaaccgctg cggtaattgt ctcaatgttg ctttccccac ttctcatttt
     1141 aatcaagcaa ggagtgagaa agaaaaaagc gaaaaatgaa tccactaata tgacaggggt
     1201 tgaattaccg gaagatttag aagccaatgt attagtcatc ggttttggac gctttaacca
     1261 aatagtctgc caagccttat tagccaaagg actaagtgta tcggttattg attcaaatac
     1321 tgatcatatt cgtgctgctg ctcgttttgg ttttaaactt tactatggtg atggagcgag
     1381 actagatgta cttagggcat ctggcatcgc caatgcaaac tgcgtaattg ttggcgtagg
     1441 caatccggaa agaacggaga aaatcgtaga gctgattaaa tctgaatacc ccttagtacc
     1501 tgtatttgcg agaacctatg atagacaaag tgcggttgat ttagtcaaaa agcaccacgt
     1561 tgattatcac attcgagaaa cctttgaatc agccctcgca ttaagccaag ccgccattga
     1621 acgcttaggt tctagcgagg aagaggcaaa acaaattatc agctatgttc gccgattaga
     1681 tgcagaacgc tttaatgagg aattattaca cggtttctcc gcagaagtta tccgcaaata
     1741 ttgggtacca acaccttggg taaatccaca acacgaaggt agtgcactta atgaagaaac
     1801 ggctgatatt ttagaagagc ttggtgagac agtagaagtt ataaaagtgg atatcactga
     1861 taaaaattaa agaatttata ctttgaatcc ttattcaagt ataagccttc ctatacatat
     1921 cgcataatca ctcgtaaaaa agcaccctat cggcataatc tgatagggtg ttaatggtaa
     1981 taaaccatcg cacgatgaca aggagaatat ctatgaaaaa aacaataaat caatttgaac
     2041 ttacattttt taagaaaatt tggcgtggag aagtcagccc taatttttct gcctcgttaa
     2101 ccaaattcat cgctttatta atatcttttg ctttaatcgc tttcattacc gcctgattaa
     2161 aataggcttc cgtatcttta tctactgctg tttttactgc aactggattt tcttttatcg
     2221 gcaacggtat agccgtttta gctgccggcg ttgctgcttt gtttgagctg aaaatttcag
     2281 tcggtaatcc aataaattta gtgccgttcg agccggtaac gttgatgtga atttgccctt
     2341 ttaaactgtg tgctacttca atatcgttaa ttgctggtgg ttgtttaccg gttgcttttg
     2401 cataggtttt tgccggatgc ggcatcatcg tggttttggc taaatcttgc tgcgtggtat
     2461 aaatgagcaa gtaaatataa tcttggttca ttgccggcgt taaattaagt tctgccccta
     2521 aacggctacc tttgagtcct cgctcttcca ataatttaaa ctcagaagag gggtaagttg
     2581 atgccacatt aaattgacta tccaacacga ctgccgtggg tacaaaaaca tttttgtcca
     2641 tcaccgggct ttcgatttca atttccaatg tgccttgatt tgccggaata cgataagctg
     2701 caaccgggct ggcaataccg gcaaacgctg tggtaaatgc ttgtttgtgt tgctcgctta
     2761 gatctgtttt tacggtttgt gaaaatggca catcttgcca ttgaatttga gctaattcag
     2821 ccgagttaat atgaatagac ttacctgccg agccatttgc tatactcgga gacaccacca
     2881 aaagatgggc gaagaatatt gctgtcgaaa gaagcgtttt tttcatatta acccctaaaa
     2941 atcgtgaaat aggataaagt gctgaaatag tgggtaggta atcctaccca cacaaaggtt
     3001 tgatgattac caccaagctt caaattgaac accggtgata aactcgccgt ctttggcttt
     3061 atagccggca tcggtacgag ctttcgtgtt gaatttatca ttccaacgtg cataagtacc
     3121 aaacacacgg attgcagggc gagcccaaat gctattacca gcctgccatt gttgtgcaac
     3181 ggtgtatttt accaagtcat ttttcttgcc tgttgcttga tctttaatgc gatcataacc
     3241 aacttctaat aatgtactca ttgtatcatt ccatttgtac attgggcgaa tacctgctga
     3301 gtaccaagtt ttaccttgtt tattatctag ttttgttttt tcgtagatta aggcatacat
     3361 tacttcaact ttatcactcg cttgcactac cccttggttg attaaacgaa gcatattacc
     3421 tttattatct gctttcgaac cctgtgcgtg cccattattc caagaagtca tagagtcggt
     3481 tgcatattgt gcagtaaatt tattgaaacc accaaagaag ttaccttgag tgtattccgc
     3541 tgttaccata taaccgtttt tggttgctcc tttttcaagg gctgcacctt tcttagcatg
     3601 tgcattaccg taatcaaagc ctaactctaa cgaaccatct ttccaaagct caataccggc
     3661 taaccgaaca tcaaaaatat cgttgtaaac atcggcttta ttacgctgct tatctaattt
     3721 aattttacct gtgctaggat ctaatttata aactgtagta tatccaaaag tattcgcccc
     3781 tccacgctct gtatcacgag taaccgctaa agagagtttt ccgaagccta aatcaatatt
     3841 ttcaacccct gcacccggac ctgaaatatc ccagtaatag aagtcattca tatgtacatc
     3901 atggcgttgg taaaaacgtt taccagccca taaggtggca cctggtaaac tatctgcaaa
     3961 gtttttgaac tgtacattta attcacgtag tgctggactt gttggtgtcc aatcattatt
     4021 gtgaagcgta ccgccgtaag caaggtttgt atcaaagtag atagatttct caccactttt
     4081 gaataattcc tgacctaatt tcaattctgc ataggtatca gattcgttac ctaaacggta
     4141 ttttgaacca ccaccattta ccgtgaatgc ggattgctcc cccccccccg atgtccaacc
     4201 gata
//
LOCUS       DANLXF010000200         4197 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.200, whole genome shotgun sequence.
ACCESSION   DANLXF010000200 DANLXF010000000
VERSION     DANLXF010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.200"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            21..1595
                     /gene="murJ"
                     /locus_tag="PXD66_002100"
     CDS             21..1595
                     /gene="murJ"
                     /locus_tag="PXD66_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDL6320358.1"
                     /translation="MSKKLLRSGMIVSFMTLISRILGLIRDIVVATILGTGVSADIFL
                     FANRIPNFLRRLFAEGAFSKAFVPVLAEYNADNDPNKTREFIAKVSGTLGGLVTVVTL
                     VAMIASPVVAALFGTGWFLDWLYDGPNAEKFTQASFLLKITFPYLWFITFVALSGAVL
                     NTIGKFGVMAFSPVLLNVAIIAVALWGRDYFDSPDIALAWGVFLGGLLQFLFQIPFMK
                     KEGLLVKPKWAWKDEGVTKVRKLMIPALFGVSVTQLNLLLNQVIASFLITGSISWMYY
                     ADRLIEFPLGLFGIAISTVVLPSLSRIAKNKELTEIQRGEHFQNTMDWGVRMVLLLGI
                     PAMIGMAVLAQPIIMTMFMRGKFGFEDVLATSYPLWVMCLGLNSYMLISVLANGFYAN
                     QNTKTPVKVGIIAALSNICFGLAFAPFLGYIGLALASACSALVNVSLLYVNLSKNGYY
                     KVSHKTVLFVLKLFIAACVMGALVAYFTPEIELWALMTMWQKVYWLIWLMILAGGSYF
                     ASILVLGIRKKDFRSA"
     gene            1597..2064
                     /locus_tag="PXD66_002101"
     CDS             1597..2064
                     /locus_tag="PXD66_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HDL6320359.1"
                     /translation="MKSLKLKIPPPAWFLLCAALMWLVAQYVPVSLPNYQHTAIFFSG
                     LAVAIIGGAVAVSALWVIHQAKTTYSPFRPQHTAKLVTWGIYRKSRNPMYLSLLLGLI
                     AWALWLGAMLAWLVLPLFVWLITYFQIKPEEQILAQKFGTHYAKYCNQVRRWL"
     gene            complement(2129..4138)
                     /locus_tag="PXD66_002102"
     CDS             complement(2129..4138)
                     /locus_tag="PXD66_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HDL6320360.1"
                     /translation="MDLIKKLNTGSTFRVPIFLPSLLFVSFVAVFCIIFPQQAQTSLD
                     TIKNSLFQHFSWFYIFAGSIFFLFLIFLSFSRLGDIKLGADTDEPEFGFGSWIAMLFA
                     AGMGIGLMYFGVAEPILHYLKPVQQNLTEPERMKEAMMTTFYHWGIHAWAIYGVIALA
                     LAYFGFRYKLALTIRSGFYPLLKHRISGFWGHLIDIIALCSTIFGLTTTLGFGVMQVS
                     AGFNNLGLIEQSNFTVLAIIVTVAMALAVLSAVSGVGKGVKILSEINLTLAGLLLIFV
                     IITGPTLLLFSSFTENLGYYFSSLLEMSFRTFAYEPEHQGWLSGWTVLYWAWWASWAP
                     FVGLFIAKISKGRTIREFILGVLFVPSLFNILWMTSFGSSAIWFDQQTAGALAEVSGN
                     TEQLLFTFFEQLPFGSIASFVAVIVISIFFITSADSGIFVLNSIASQGEENAPKWQSV
                     LWGALLAILALSLLYSGGLASLQTMTLIIALPFTFIMLILCIGLWKGLMVDNQYFNKK
                     FSQGSQHWAGKDWKQRLEKIINPSNKQDVRHFFIKVARPAFLELIEEFESYGLIAKMN
                     FTNEQNPKLEFEVVKENLRNFIYGIESVPRELSDLVVGDDNLPNIEQNTIYEPITYFL
                     DGRKGYDVQYMTKEELIADVLQQYERFINLAMDNSHDLMTADFNH"
BASE COUNT         1188 a          839 c          934 g         1236 t
ORIGIN      
        1 tactaatatt actctttatt atgagcaaaa aacttttaag atcaggaatg atcgtaagct
       61 ttatgacctt aatttctcgc atactcggtt tgattcgaga cattgtggtg gcaaccattt
      121 taggcacagg cgtgagtgcc gatattttcc tgtttgccaa ccgtattccc aattttcttc
      181 gccgtttgtt tgccgaaggg gcattttcta aagcctttgt gccggtgttg gcggaatata
      241 atgcggataa cgatccaaac aaaacgagag aatttattgc caaagtatcg ggtacattag
      301 gaggtttggt aactgttgtt acccttgtgg caatgattgc ctcgcctgtg gttgccgctc
      361 tttttggtac aggttggttt ttagattggc tttacgacgg gccaaatgcc gaaaaattca
      421 cccaagcctc ttttttgctc aaaatcactt tcccttattt gtggtttatt acctttgtgg
      481 cgttatccgg tgcggtgtta aataccatcg gtaagtttgg ggtgatggca ttttccccgg
      541 tgttgctgaa tgtggcgatt atcgcggtag cgttatgggg cagagattat ttcgattccc
      601 cggatattgc cttagcgtgg ggggtatttt taggcggctt attgcaattt ttattccaaa
      661 ttccatttat gaaaaaagag ggcttactgg ttaagccaaa atgggcgtgg aaagacgaag
      721 gtgtcactaa agtccgtaaa ttaatgattc cggctttatt tggcgtttcc gttacccagc
      781 taaatttact gctaaaccaa gtgattgcct cgtttttgat taccggctcg attagttgga
      841 tgtattatgc cgaccgctta attgaatttc cgctcggttt atttggaatt gcgatttcca
      901 ccgtcgtgct gccaagcctt tcccgtattg cgaaaaacaa agaactcact gaaatccaac
      961 gtggagaaca ctttcaaaac acaatggatt ggggggtgag aatggtgctg ttgctcggca
     1021 ttccggcaat gatcggtatg gcagtgctgg ctcagccgat tattatgaca atgtttatgc
     1081 ggggtaaatt tggttttgaa gatgtgctgg caacctctta tccactttgg gtaatgtgct
     1141 tggggttaaa cagctatatg ttgattagcg tgctggcaaa cggtttttat gccaaccaaa
     1201 acaccaaaac accggtaaaa gttgggatta ttgccgcctt aagtaatatt tgctttggct
     1261 tggcgtttgc tccgtttttg ggatacattg gtttagcgtt agcttctgcg tgttcggcat
     1321 tagtgaatgt gagcttgctg tatgtgaatc tctcaaaaaa tggttactat aaagtaagcc
     1381 ataaaaccgt gctttttgtg ttgaaactat tcatcgccgc ttgtgtaatg ggggcgttgg
     1441 ttgcctattt cacgcctgag attgaacttt gggcattaat gacaatgtgg cagaaagtgt
     1501 attggctgat ttggctgatg atcttagccg gcggtagtta ttttgcctct attttggtat
     1561 taggcattcg taaaaaagat ttccgctcgg cataaaatga aatcattaaa actgaaaatc
     1621 ccgccgccgg cttggttctt gctttgtgcc gctttaatgt ggctggtagc tcaatatgtg
     1681 ccggtgagct tgccgaatta tcagcataca gccatatttt ttagcggttt agctgtggcg
     1741 ataattggcg gagcggttgc cgtctcggca ctttgggtca tacaccaagc gaaaacgaca
     1801 tacagtcctt ttcgaccaca acataccgct aaattggtca cttggggcat ttaccgcaaa
     1861 agccgaaacc caatgtatct ttcgttacta ttaggtttga ttgcgtgggc attatggttg
     1921 ggggcaatgt tggcgtggct ggttttaccg ctgtttgtat ggttgataac ctatttccaa
     1981 atcaagccgg aagagcaaat tttagcccaa aaattcggca cacattatgc caaatattgc
     2041 aaccaagtaa ggcgttggct ttaaatgcaa gcggtgagat tttgttggaa atttgcaaat
     2101 ttttccgaaa atctcaccgc ttgtggggtt agtgattgaa atcagccgtc attaagtcgt
     2161 gcgagttgtc catcgctaaa ttgataaagc gttcatactg ttgcagcacg tcggcaatca
     2221 actcttcttt ggtcatatat tgcacatcat aacctttccg cccgtctaag aaataagtaa
     2281 tcggctcgta aatggtattt tgctcaatgt tcggtaggtt gtcgtcacct accaccaaat
     2341 ccgataattc ccgtggcaca ctttcaatgc cgtaaatgaa attgcgtaaa ttttctttca
     2401 ccacttcaaa ctctaatttc gggttttgtt cgttggtgaa attcatttta gcgattaagc
     2461 catagctttc aaattcctcg ataagttcta aaaatgctgg tctggcaact ttaataaaga
     2521 agtgacggac atcttgctta ttgcttgggt tgatgatttt ctccaagcgt tgtttccaat
     2581 ctttacccgc ccaatgttgg ctaccttgcg agaatttttt gttgaagtat tggttatcta
     2641 ccattaatcc tttccataag ccgatacaga gaatcagcat aatgaaggta aatggtaagg
     2701 cgataatcag tgtcattgtt tgcagagaag ccaagccacc cgaatagagt agtgataacg
     2761 ctaagatggc taataatgct ccccaaagca cgctttgcca tttcggtgca ttttcttcgc
     2821 cttgtgaagc aatgctgttg agaacaaaaa tccccgagtc ggcagaggtg ataaagaaaa
     2881 tactgataac aatgacggca acgaaagagg caatagagcc aaacggtaat tgctcaaaaa
     2941 aggtaaataa cagttgttcg gtattgccgc tgacttcagc taaagcaccg gcagtttgtt
     3001 gatcgaacca aatggcagag ctgccgaagc tggtcatcca taaaatgtta aacagcgatg
     3061 gaacaaatag cacccctaaa ataaattcac gaatggttct gcctttagag atcttggcaa
     3121 taaacaaacc aacaaatggc gcccaagatg cccaccatgc ccaataaagg accgtccagc
     3181 cgcttagcca gccttgatgt tccggttcat aagcgaaggt acggaaactc atctcaagca
     3241 gcgagctaaa ataatagcct aaattttcgg tgaagcttga gaaaagtaat agagttgggc
     3301 cggtgattat cacaaaaata agtagcaatc cggctaatgt gagattgatt tcacttaaga
     3361 ttttaacccc tttgcctacg cccgaaacgg cagataacac ggcaagagcc attgctactg
     3421 ttacgataat cgcaagaaca gtaaaattgc tctgttcaat taaacctaga ttgttaaagc
     3481 cagcactgac ctgcatcacc ccaaagccaa gtgtagtcgt taaaccgaaa atcgtgctac
     3541 aaagggcaat aatatcaatt aaatgccccc agaagcctga aatacgatgt tttagtaagg
     3601 gataaaatcc ggaacgaata gtgagtgcta acttatatct gaagccaaaa taagcaagag
     3661 ctaaggcaat cacaccataa attgcccaag cgtgaatacc ccaatgatag aacgttgtca
     3721 tcatcgcttc tttcatacgc tccggctcag ttaaattttg ttggacgggt ttaaggtaat
     3781 gcaaaatagg ttctgctacc ccaaaataca ttaacccgat ccccattccg gcggcgaata
     3841 acatcgcaat ccaagagcca aaaccaaatt caggctcatc ggtatctgcc cctaatttaa
     3901 tatcacccaa tcggctgaaa gagagaaaaa ttagaaacag gaaaaagata gagcctgcaa
     3961 aaatatagaa ccagctaaaa tgttggaaga gactattttt gatggtatca agtgaggttt
     4021 gtgcttgctg tggaaagatg atacagaaaa cggcaacaaa gctgacaaag agtaaactcg
     4081 gtaggaaaat cggtacccta aaggtacttc ctgtgtttaa ttttttgatt aaatccaatg
     4141 ctatttcctc tatttttgaa tggttagagg cttgaatata gcaaaaagat ataaaaa
//
LOCUS       DANLXF010000201         4195 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.201, whole genome shotgun sequence.
ACCESSION   DANLXF010000201 DANLXF010000000
VERSION     DANLXF010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.201"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            26..817
                     /gene="tpiA"
                     /locus_tag="PXD66_002103"
     CDS             26..817
                     /gene="tpiA"
                     /locus_tag="PXD66_002103"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDL6320361.1"
                     /translation="MARRPLVMGNWKLNGDKAFTKTLIADLKAELHGVEGCDVAIAPP
                     VMYLAHAEASLAGCGCGCSDKGLIALGSQNVDVNVKGAFTGDISPEMLKEFGAKYIII
                     GHSERRTYHKESDEFIAQKFKVLKDAGLVPVLCIGESEAENEAGKTEEVCARQIDAVI
                     NAHGVEAFNGAVIAYEPIWAIGTGKSATPAQAQAVHKFIRDHIAAKSQAVADQVIIQY
                     GGSVNDANAAELFTQPDIDGALVGGVSLKAPAFAVIVKAAAAAKA"
     gene            complement(903..1388)
                     /locus_tag="PXD66_002104"
     CDS             complement(903..1388)
                     /locus_tag="PXD66_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HDL6320362.1"
                     /translation="MTNTILILFILLGLVFAIYDQVFMHKLKGTTLLEIRLKKQKTID
                     TWLLIGLIVLSISYGVQNQIQPFTLYLLATCIILSVYLAFFRSPRLLLKQHGFFFANV
                     FFNYDKIYQVNIAEGKADEKILVIDLHSGRRLLAYVENAEDLQPVVDFFGGYKKESEQ
                     K"
     gene            complement(1481..2317)
                     /locus_tag="PXD66_002105"
     CDS             complement(1481..2317)
                     /locus_tag="PXD66_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005564636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HDL6320363.1"
                     /translation="MTTEIKKVTKSDLNAVVRRSNLFQGSWNFERMQALGFAYAMVPV
                     IKRLYPDPNGPGRKEAIKRHSEFFNTQPFVGAPILGVTIAMEEERANGKEIDNAAING
                     IKVGLMGPLAGVGDPIFWGTVRPVFAALGAGLALKGELLGPILFFALFNIVRLAARYY
                     GVVYGYKKGLDVVQDMSGGLLQKLTEGASILGLFIMGALVQKWTSINVPLVLSTIQKQ
                     DGTIEVTTVQSILDSLMPGLLPLLLTFACMWLLRNRVNAIWIILGFFVIGILGAATGA
                     LA"
     gene            complement(2331..3131)
                     /locus_tag="PXD66_002106"
     CDS             complement(2331..3131)
                     /locus_tag="PXD66_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005716709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HDL6320364.1"
                     /translation="MELSALQIVLLFLVACISGMGSILDEWQTHRPLIACTMIGLVLG
                     DVTTGVIVGGSLELLALGWMNIGAAVAPDAALASIVSTILVIVGRQDISTGIAIAIPL
                     AAAGQVLTYVVRAITVGFQHAADKSIEDGNLSRLDWLHRGALLLQAMRIAIPALIVAL
                     TAGTDAVQTMLNAIPEVVTTGLKIAGGFIAVVGYAMVINMMRAGHLMPFFFAGFVIAG
                     FTDFNLVALGVLGTVIAMVYIHMHPKYNKSQQVVVAAAPKNDLDNRLD"
     gene            complement(3143..4120)
                     /gene="manX"
                     /locus_tag="PXD66_002107"
     CDS             complement(3143..4120)
                     /gene="manX"
                     /locus_tag="PXD66_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDL6320365.1"
                     /translation="MSIAIIIATHGVAAEQLLKTTEMLIGEQEDVAYIDFVPGENAET
                     IMGKYQALVEGPLSHCDQVLFLVDMWGGSPFNAANRFQDGKSGMDVVAGVNIPMLVNT
                     CMARDDVESLEELVEVALEAGSTGIRSLNHQPEVAEVQTATPAQETAAVQQPEIVDDG
                     RRMTIALARIDDRLIHGQVATVWTKESKVNRIIVVNEGVAKDSVRTTMLKSVAPLGVT
                     AHVVDVPKMIRVYNNPEYADDRVMLLFTNPSDVLALIEGGVPLNSINVGGISHKEGKQ
                     MVSHSVAVDQNDIDAFKAIDAKGVELEVRKVSSDSRQYMMDLLKKHNLI"
BASE COUNT         1260 a          913 c          855 g         1167 t
ORIGIN      
        1 ttcaacttca tataggagaa acaaaatggc acgtcgtcca cttgtaatgg gtaactggaa
       61 attaaacggt gataaagcgt ttactaaaac cttaattgca gatttaaaag cagaattaca
      121 tggcgttgaa ggctgtgatg tagcaatcgc tccaccggtg atgtatttgg cacacgctga
      181 agcatcactt gccggttgtg gctgtggttg cagtgataaa ggcttaatcg cacttggttc
      241 acaaaacgtt gatgtgaacg taaaaggggc attcaccggt gatatttcac ctgaaatgtt
      301 aaaagagttt ggggcaaaat atattattat cgggcattca gaacgccgta cttaccacaa
      361 agaaagcgat gaatttattg ctcaaaaatt caaagtatta aaagatgccg gcttagtgcc
      421 tgtattatgt atcggtgaat ctgaagccga aaatgaagca ggtaaaactg aagaagtgtg
      481 tgcaagacaa attgatgcgg tcatcaacgc gcacggtgtg gaagcattca acggtgcagt
      541 tattgcttac gaaccaattt gggcaatcgg taccggcaaa tcagcgactc cggcacaagc
      601 acaagcagtg cataaattta ttcgtgacca tattgcagct aaatcgcaag cagtggctga
      661 ccaagtgatc attcaatacg gtggctctgt aaacgatgcc aatgcagcag agttattcac
      721 tcaaccggat attgacggtg cattagtagg cggtgtttca cttaaagcgc ctgcttttgc
      781 ggtgatcgtt aaagcggcag cggctgctaa agcctaattt aaccgatttt acaagcggtc
      841 atttttcctc agttttttgc aaaatttcag ggaaaaatga ccgcttgtgt tttgttcgca
      901 aactatttct gctcactttc ctttttataa ccgccaaaaa aatccactac cggttgaaga
      961 tcttccgcat tttcaacata ggcaagcagc cttcttccgc tatgtaaatc aattaccaaa
     1021 attttttcgt ctgcttttcc ttctgcaata ttgacttgat aaatcttgtc gtaattaaaa
     1081 aagacattcg caaagaaaaa accgtgctgt tttaacaaca agcgagggga acggaaaaag
     1141 gcgagataga ccgacagaat aatgcaagtc gccagcaggt aaagtgtaaa aggctggatt
     1201 tgattttgta cgccatagct aattgacaag acgattaaac caatcaataa ccaagtatca
     1261 attgtttttt gcttttttaa acggatttcc agtaaggttg tgcctttgag tttgtgcata
     1321 aaaacttggt cataaatggc gaatactaag ccaagtaaaa taaagagaat aaggatggta
     1381 ttggtcataa ttaagcaagt gaatttattt gggctaaagc gtataaaaaa ccgcttgaat
     1441 gttcaagttc aagcggtcgt tttcacagaa aattttgcaa ttaagctaat gcaccggttg
     1501 cagcgccgag gataccgata acaaagaaac cgaggataat ccagatagca tttactcgat
     1561 tacgaagtaa ccacatacaa gcaaaggtta ataataatgg gagtaggccc ggcattaagc
     1621 tgtcgagaat gctttgcact gtggtaactt cgatggtacc gtcttgtttt tgaatggttg
     1681 aaagcacaag cggaacgtta atgctcgtcc atttttgtac caatgccccc ataataaaca
     1741 gacctaagat tgaagcacct tcggttaatt tttgcaataa accgccgctc atatcttgta
     1801 caacatctaa ccctttttta tagccataca ctacgccata atagcgtgcg gctaaacgca
     1861 ctatgttaaa taaggcgaaa aacaggattg ggcctaaaag ctcacctttt aaggctaagc
     1921 ctgctcctaa tgcggcaaat acagggcgaa cagttcccca gaaaattggg tcacccacac
     1981 cggctaatgg tcccattaaa ccaactttaa taccgttaat tgcggcattg tcgatttctt
     2041 taccgttggc acgttcttct tccatagcaa tggttacccc taaaatcggt gcaccgacga
     2101 atggctgggt gttaaagaac tcagaatggc gtttaattgc ttctttgcga cccgggccat
     2161 ttggatccgg atataaacgt ttgataaccg gtaccatcgc atacgcaaaa ccaagagcct
     2221 gcatacgctc aaagttccac gaaccttgga ataagtttga acgacgtaca acggcattca
     2281 aatcgctttt tgttactttt ttgatttcag ttgtcatttt aaatgccctc ttagtctaaa
     2341 cggttgtcta aatcattttt tggtgcagct gccaccacta cctgttggct tttgttatat
     2401 ttagggtgca tatggatata aaccattgca ataaccgtac ctaatacccc aagggcaaca
     2461 agattgaaat cggtaaagcc tgcaattaca aagcctgcga agaagaacgg cattaaatga
     2521 cctgcacgca tcatattgat caccatcgca taacccacca cggcgatgaa gccaccggca
     2581 attttcagcc cggttgtaac cacttcagga atggcattta acattgtttg aaccgcatct
     2641 gtacctgctg ttaatgccac aattaatgcc ggaatggcaa tacgcattgc ttgaagcaat
     2701 aaagcaccac gatgtaacca gtctaaacgg cttaagttac cgtcttcaat tgatttatct
     2761 gctgcgtgtt ggaaacctac ggtgattgca cgcacaacat aggttaatac ttgaccggca
     2821 gctgcaagcg gaatagcgat ggcgatacca gttgaaatat cttgacgacc tacaatgact
     2881 aaaatggttg agacaataga ggctaatgcc gcatctggtg caacagcggc accgatgttc
     2941 atccaaccta aagctaataa ctctaatgag ccaccaacaa taacgccggt agtcacatca
     3001 ccaagcacta agcctatcat cgtacaggca attaacggac ggtgggtttg ccattcatca
     3061 agaattgagc ccatacctga gatacaggca acgaggaata aaagcacaat ttgtaatgct
     3121 gaaagttcca tagtaatttc ctttagatta agttatgttt tttcaataaa tccatcatat
     3181 actgacggct atcgcttgag actttgcgta cttctaattc aacgcctttc gcatcaattg
     3241 ctttgaaggc atcaatatca ttttgatcaa ccgccacaga atggctaacc atttgtttcc
     3301 cttctttatg cgaaataccg cccacgttaa tgctattgag tggtacaccg ccttcaatta
     3361 aggctaagac atcacttggg ttggtaaaga gtaacattac acgatcatcg gcatattcag
     3421 ggttgttata gacacgaatc attttaggta catcaacaac gtgagccgtt acgccaagag
     3481 gagcaacact ttttagcata gtagttcgta cactatcttt tgctacacct tcgtttacca
     3541 caataatgcg attgacttta ctttcttttg tccaaaccgt cgcaacttga ccgtgaatca
     3601 agcggtcatc aatacgggca agggcaatgg tcatacgacg tccgtcatca acaatttcag
     3661 gctgttgaac agctgcagtt tcttgtgcag gagtagcggt ttgcacctca gccacttcag
     3721 gttggtgatt aagtgaacga atcccggttg agccggcttc taatgcgact tccaccaatt
     3781 cttccaacga ctccacatca tcacgagcca tacaggtgtt cactaacata ggaatattca
     3841 cacccgcaac aacatccata cccgattttc catcttggaa tcggtttgcg gcgttgaatg
     3901 ggcttcctcc ccacatatcc accaaaaaca gcacttgatc acagtgtgat agtggacctt
     3961 caaccaatgc ttgatatttg cccataatcg tttctgcatt ttcgccggga acaaagtcaa
     4021 tgtaagcaac atcttcttgt tcaccaatca acatctcagt tgttttgaga agttgctctg
     4081 cagcaacacc atgggttgct atgataatag caatgctcat ttgttactcc ttgcggtttt
     4141 ttaagttgtt tatttactgg ggcgagattt taatatgata attatttcac acaat
//
LOCUS       DANLXF010000202         4181 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.202, whole genome shotgun sequence.
ACCESSION   DANLXF010000202 DANLXF010000000
VERSION     DANLXF010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.202"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(65..3355)
                     /gene="recC"
                     /locus_tag="PXD66_002108"
     CDS             complement(65..3355)
                     /gene="recC"
                     /locus_tag="PXD66_002108"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HDL6320366.1"
                     /translation="MFTLYQSNQISSLAEMLVKIQQVNPLEDPFEPETILIQSQGMAQ
                     WLQMQIAELNGVMGNCDFLYPTTFLWQQYRLLFPELPKENIFERSSLVWRIMRLLPSC
                     LTLSEFAPLKRYLGDQPNKEQYQLKLYQLSAKIAELFDQYLVYRPHWLVSWEEGNLQA
                     IENEIKIATAAKPIDFDLIRADMKWQSHLWQQIVSDLQSESDEQVFTTSHRAYLQQNY
                     FAKLDDLTETEKAKLPKRIFIFGISSFSPLQLAVFKKLSEHCDIHLFFFNPSEKYWGD
                     SVEEKVWQKLALSHKISQDDLDNLLAEQRNKLLNLWGKQGRDFLAQLIEFEPNTMDVF
                     VEPEENSNLNKLKKQIFSLENDATITDLTADNSLQIHSCHSPMREVEVLHNQLLNLFE
                     QDSTLLPKDIIVMSPDINKYAPYIDAVFSRYNTGNRDPRYIPFSLSDQAFTQVDPIIH
                     SFLQLLAMKESPFNAEELFDLLEVNAIQTRFGFSTEDIHTLRVWVRNAGVRSGVEIEQ
                     PHWQNYNSWENGLNRLLLGSSLKEEQGIWENTLAFGDSYGLNAELVGRLASFIEALLA
                     WVNFLQTPQPLAAWQQAIKNLTADLYQENAENAASILLLNNVNDQIIEQISATHFSDD
                     LQIEVLALLFEQQLSQHSQQLNFLVGRVNFATLLPMRAIPFKVICLLGMNEADFPRQQ
                     QINSFDLMQYAPKKGDRAKRDDDRYLFLEALLSAQQMFYISYIGQSQKDGKEMLPSIL
                     VSQLLDAMNEALTPECKQQLSDEVEVLVRKQPLSVFSKKNFTHPRDHAYNAEWIIKPT
                     VAVQDFLSEPLTNESVASLELDELISFVQNPIRFFFLKVLGISFNTYDDTIDESEIFE
                     MSGLDNYRILNELLTINLAQQQRFFAEEQLKGNLPASYFAQIATQDLIGKITEMQAAL
                     SHYLASESRILSVNREVELERQTLTLNGNIANLYQNEVVLWRVGHLRDKDKIQTWIYH
                     LLLCAYFPEKTVKFYYREGEQVGVLSFETVSQIEALSQLSIYLNAYLDGLSQAQLVIY
                     DRIEEYLKGDGTNSEQVLIDLAEKDERDGSYLHRLLAQTKELDSQAIHQRTQSWFSLM
                     NQKIAKGD"
     gene            complement(3404..3697)
                     /locus_tag="PXD66_002109"
     CDS             complement(3404..3697)
                     /locus_tag="PXD66_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5374 domain-containing protein"
                     /protein_id="HDL6320367.1"
                     /translation="MIKYKAETALSLLVAITLFAIVVLSFSQWQSAQNHRLNQHFQQQ
                     QAVMILENQLALKLAGLDCESSITQNQIRYQIQCSGNRLTIRFPLGKIELNND"
     gene            complement(3690..>4181)
                     /locus_tag="PXD66_002110"
     CDS             complement(3690..>4181)
                     /locus_tag="PXD66_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2572 family protein"
                     /protein_id="HDL6320368.1"
                     /translation="QKFQKEQISQADLSDKQFRFLQYQFACQFHSLFKDKKPTKEKYI
                     HFTHLADYLDLSNVPNQEIYTIHSLAELPSSSEREPKIVLAKNAINENLPQEFYGIVI
                     TDYLFDITGKKMYGTLYSSYDNAREERNLTFKKEVLAELEARYSAWRYLPYSEHLMGA
                     VDD"
BASE COUNT         1178 a          914 c          834 g         1255 t
ORIGIN      
        1 ggtaggcgaa ttattattcg ccttctcatt ttgcaaattt tcagccaaaa acgaccgctt
       61 gtagttaatc gcctttcgct attttctgat tcattaagct aaaccagctt tgagtacgtt
      121 ggtgaatcgc ttgagaatcc agctctttag tttgagcgag aagccgatgg agatagctgc
      181 cgtctcgctc atctttctct gctaaatcga taagaacttg ttcgctgttt gtgccatcac
      241 ctttgagata ttcctcaatt cggtcgtaga tcactaactg ggcttgcgat aatccgtcca
      301 aataagcatt caaataaatg cttaattgcg ataatgcctc tatctgcgag acggtttcaa
      361 agctcaacac accaacttgc tcaccctctc ggtaatagaa tttcaccgtt ttttcaggga
      421 aataggcaca caataataaa tggtagatcc aagtctgaat cttgtcttta tctcgtaaat
      481 gccctactcg ccatagcacc acttcattct gatataggtt ggcaatattg ccgtttaggg
      541 tgagggtttg gcgttccagc tcaacctccc gattaacact caaaatacgg ctttcgctgg
      601 ctaaatagtg gcttaacgcc gcttgcattt cggtaatttt gccgatcaaa tcttgtgtgg
      661 caatttgagc aaaataagaa gcaggtaaat tgcctttgag ttgctcttcg gcaaaaaaac
      721 gttgctgttg tgctaaattt atcgttaata attcgtttaa aatccggtag ttatccagcc
      781 ccgacatctc aaaaatttcg ctttcatcaa tggtgtcatc ataagtgtta aacgaaatcc
      841 ccaacacttt tagaaagaaa aacctaatcg ggttttgtac aaaagagatt aattcatcaa
      901 gttctagcga agccacgctt tcattggtta aaggctcact aagaaaatct tgtacggcaa
      961 cagtcggctt gataatccac tctgcgttat aagcgtgatc tcgtgggtgg gtaaaattct
     1021 ttttgctaaa cacacttaaa ggttgttttc tgaccaaaac ttccacttca tcgcttaatt
     1081 gttgcttgca ttcaggcgtt aaagcttcat tcattgcatc taacagttgg gaaaccaaaa
     1141 tcgacggtag catctctttg ccgtcttttt gcgactgccc gatatagctg atgtaaaaca
     1201 tttgctgagc ggaaagtaag gcttctaaga acaagtagcg gtcatcatca cgtttggcac
     1261 ggtctccctt tttcggggcg tactgcatta aatcaaagct attgatttgt tgttggcgag
     1321 ggaaatctgc ctcattcata cccagcaagc aaattacctt aaacggaatc gcccgcatcg
     1381 gaagcaaggt ggcaaaattc acccgtccga ccaagaaatt aagctgctgt gaatgttggc
     1441 tgagctgctg ctcaaacaat aaggctaaaa cctcaatttg cagatcgtct gaaaaatggg
     1501 ttgcggaaat ttgctcgata atctgatcat tcacattatt caacaataaa attgatgcag
     1561 cattttcggc attttcttga tacagatcag cagtcaaatt ttttattgcc tgctgccacg
     1621 ctgccaacgg ctgtggcgtt tgcagaaagt ttacccacgc taataaagcc tcaataaaag
     1681 aggcaagtcg ccccaccagc tcggcattta agccatagct atccccaaag gcaagggtgt
     1741 tttcccaaat cccctgctct tctttcaggc ttgagccaag caataaacgg tttagcccgt
     1801 tttcccacga attgtagttt tgccagtgag gttgctcgat ttctacaccc gaacgtacgc
     1861 cggcatttct cacccaaacc cgcagggtat gaatatcttc cgtactaaag ccaaaacggg
     1921 tttggatcgc attcacttcc agtaaatcaa acagttcttc ggcattaaac gggctttctt
     1981 tcattgccag cagttgcagg aaactgtgga taatcggatc aacctgcgta aatgcttggt
     2041 cggataatga aaaaggaata tagcgaggat cccgattacc cgtattgtag cgagaaaaca
     2101 ccgcatcaat gtaaggagcg tatttattaa tatccggcga catcacaata atatctttcg
     2161 gcagcaaagt gctatcttgc tcaaacaaat tcaggagctg gttatgcaac acttccactt
     2221 cccgcattgg gctgtggcac gaatgaattt ggagtgaatt atcggcggtt aaatcggtaa
     2281 tagtggcgtc attttctaag ctgaaaatct gttttttgag tttattgagg ttgctgtttt
     2341 cctctggttc gacaaagaca tccatggtat tcggctcaaa ttcgattaat tgagctaaga
     2401 aatcccgccc ttgtttgccc caaagattca gtagtttatt tctttgctcc gccaataagt
     2461 tatccagatc gtcttgcgaa atcttgtggc ttaacgccaa tttttgccac actttttctt
     2521 ccacactgtc gccccaatat ttttcgcttg ggttaaaaaa gaacaggtga atatcgcaat
     2581 gctcgctgag tttcttaaac accgctaact gtaatggcga gaaggacgaa atcccaaaaa
     2641 tgaaaatgcg ttttggcaat ttggcttttt ccgtttcagt aaggtcatct aacttagcga
     2701 agtaattttg ttgcaaatag gcacggtggg aggtagtaaa aacctgctca tcagattccg
     2761 attgcagatc agatacaatc tgttgccata aatggctttg ccatttcata tcggctcgaa
     2821 ttaagtcaaa atcaatcggc ttagcggcag ttgcaatttt tatctcattt tcgatcgctt
     2881 gtaagttgcc ctcttcccag ctaaccagcc aatgggggcg atacaccaaa tattggtcga
     2941 acaattcggc aattttggca gaaagttgat agagcttaag ctgatactgt tctttattag
     3001 gttgatcacc taaataacgc tttagcggag caaactccga tagtgtcaag caactcggca
     3061 ataaccgcat aatccgccaa accaacgaac tacgctcaaa aatattctct ttcggcagct
     3121 ccgggaacaa cagccgatac tgttgccata aaaatgtagt agggtagaga aaatcgcaat
     3181 tccccatcac accgtttaat tcagcaatct gcatttgtag ccactgtgcc attccctgac
     3241 tttggatcaa aatggtttcc ggctcaaacg gatcttcaag tgggtttacc tgctggattt
     3301 tcaccagcat ctccgccagt gatgaaattt ggttggattg gtaaagtgta aacataataa
     3361 cgcctcgcta attgaattat tgtcctccaa agcgagggac gattcaatca ttattcagct
     3421 caattttgcc taacggaaag cgaatggtta agcgattgcc agagcattga atctgatagc
     3481 gaatctgatt ttgagtaata cttgattcac aatctaaccc ggctaatttg agggcaagct
     3541 gattttctaa aatcataacc gcttgttgct gttggaaatg ttgatttagc ctgtggtttt
     3601 gtgccgattg ccactggctg aaactcaaca ccacaatcgc aaatagcgtg atggcaacca
     3661 aaagcgacaa agccgtttct gctttatatt taatcatcaa ccgctcccat caaatgttcg
     3721 gaatatggca aatatcgcca agcggaatac ctcgcctcaa gctctgccag tacctctttt
     3781 ttaaacgtta aatttcgctc ctctcgggca ttatcataag aggaataaag cgtgccgtac
     3841 atttttttgc cggtaatatc aaacagatag tcggttatca caatgccgta aaactcttgc
     3901 ggtaagtttt cattaattgc gtttttagca agcacaattt taggctcacg ttcactgcta
     3961 ctaggtaatt cagctaaaga atgaatagta taaatctctt ggtttggcac atttgataaa
     4021 tctaaataat ccgccaaatg ggtaaagtga atatatttct ctttagtcgg ttttttatct
     4081 ttaaataaac tatggaattg gcaggcaaat tgatactgta aaaacctgaa ttgtttatcg
     4141 gataaatccg cttggctgat ctgctctttt tgaaattttt g
//
LOCUS       DANLXF010000203         4178 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.203, whole genome shotgun sequence.
ACCESSION   DANLXF010000203 DANLXF010000000
VERSION     DANLXF010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.203"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            39..302
                     /locus_tag="PXD66_002111"
     CDS             39..302
                     /locus_tag="PXD66_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HDL6320369.1"
                     /translation="MKIYTWDETKRKINLAKHGLDFADADEVLSSPYRFDVEVIRNNE
                     FRIQSFAYSFGRLLVLSLAYTEREDYIRVISFRRASKAEREAY"
     gene            358..603
                     /locus_tag="PXD66_002112"
     CDS             358..603
                     /locus_tag="PXD66_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HDL6320370.1"
                     /translation="MAIQAIPASQDFIWDGKDDDERPVTPSEFSIAMKKRGRPAGSKK
                     ELVSLRLDKSVLEAFRASGKGWQSRINQVLSDFVKQV"
     gene            complement(621..914)
                     /locus_tag="PXD66_002113"
     CDS             complement(621..914)
                     /locus_tag="PXD66_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3144 domain-containing protein"
                     /protein_id="HDL6320371.1"
                     /translation="MTQLSTSVYQRADGFINVANAHLKNIAPNQVSNAMLFGCARFNA
                     YVAASKAEYKQQLADSREEVIQYFVEQYKEMLTANLDEYIQNFERYIEGKKAD"
     gene            complement(1080..2039)
                     /gene="rpoH"
                     /locus_tag="PXD66_002114"
     CDS             complement(1080..2039)
                     /gene="rpoH"
                     /locus_tag="PXD66_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HDL6320372.1"
                     /translation="MKKFNENEIEDAEIIDAEPIDVDEIDEEETSVEAMHPALVSGSM
                     PVPQGNLDSYIRMANQYPILTAEQEKELAERYYYDEDVEAAKQLILSHMRFVIHIARG
                     YLGYGLPLADLIQEGNIGLMKAVKRFDPNVGVRLVSFAVHWVKAEIHEYVLKNWRIVK
                     VATTKAQRKLFFNLRKNKNRLAWFNEEEIKKVADDLGVSVEEVREMESRMTGQDLGFD
                     LPAGDDDEDSYAPSMYIEDSSSNFADDLEDEQHNGQATAQLAYALATLDERSQDIIKT
                     RWLDDNKATLQDLADKYNISAERVRQLENAALKKIKEAISLDG"
     gene            2205..2571
                     /gene="ssrA"
                     /locus_tag="PXD66_002115"
     tmRNA           2205..2571
                     /gene="ssrA"
                     /locus_tag="PXD66_002115"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            2846..4072
                     /locus_tag="PXD66_002116"
     CDS             2846..4072
                     /locus_tag="PXD66_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL6320373.1"
                     /translation="MARKITPLNDTQIRKAKPEDSPLRDGNGLLLVITSNSKLWRFRY
                     ERPFTKKRNDLSIGAYPDISLAQAREIREEYRALLAQNIDPQTHRQQQEQAKANEISN
                     TYENVAWAWLEHRKTKKNFSDNYQKDVIALIKNNLLPHFGRMPITQITAPMAIKAFKQ
                     YQDEGKLEKLKRAIQKHNEIMMFAVHREMIPFNPTANIAKEFDSPTVEHFKTIKPEDL
                     GEFLYTLQNAQIHLQTRYLILWQLLTMTRPNEAAKAKWADIDEKAKLWTIPAENMKRG
                     IEHKITLSRQAIALLREIKKLSGGKTYLFPSVKNPQTHVNTQTANGAIKRMGYKGKLV
                     AHGLRAIASTYLNEKGYNSDLIEVALSHLNSDRIKAAYDRGERLEQRFKLLQVWADFV
                     EECSQGSLPQYHLKIA"
BASE COUNT         1275 a          906 c          828 g         1169 t
ORIGIN      
        1 agttcttgtt agatttattt tatgtatata caataattat gaaaatttat acttgggacg
       61 aaactaaacg caaaatcaat ttagccaagc acggattaga ttttgccgat gcagatgaag
      121 ttctctcatc gccctatcga tttgatgttg aagttattcg taacaatgaa tttagaatac
      181 aatcttttgc atattcattt ggacgtttac tcgtattatc acttgcatat acagaaaggg
      241 aagattacat cagagtaatt agctttcggc gagcatctaa agcagaaaga gaggcatatt
      301 aggaatactt acaacaatac tttgaataat aaaaatttag agaaaaaaca gctacttatg
      361 gctattcaag ctattccggc aagccaagat tttatttggg acggaaaaga tgacgatgaa
      421 cgcccggtta cgccatcaga gttttcaatt gcaatgaaaa aacgtggcag accagctggt
      481 tcaaagaaag aattggtttc gttacgtctt gataaaagcg ttcttgaagc ctttagagct
      541 tcaggtaaag gttggcaaag tagaattaat caagttttaa gtgattttgt aaaacaagtt
      601 tgaccacaaa acaaaggcgg ttaatccgcc tttttccctt caatatatcg ctcgaagttt
      661 tggatatatt catctaaatt tgcagtgagc atttctttgt attgctccac aaaatattga
      721 ataacttctt ctctggaatc ggccaactgc tgtttatatt ccgccttgct tgccgcaaca
      781 taggcgttaa atctggcaca accaaacaac atcgcattgc tgacttggtt tggggcaatg
      841 ttttttaaat gagcgttggc aacgttaata aagccgtctg ctcgctggta gacgctggta
      901 gaaagttggg tcaatttgtt tatcggtaat gttaatcata gtatcctcat ttttagtcaa
      961 aatacaagcg gtgctttttg cgtaaaattt tgcacaaacg cttgcgtttg aacgttggct
     1021 ctgccgacgg cacgaagtgc gagcgtagcg agtaaaaaaa tcaccgcttg taggcatttc
     1081 tagccgtcta atgaaatcgc ttccttgatt ttcttcaatg cggcattttc aagctgtctt
     1141 acccgctctg ccgagatgtt atatttatcg gctaaatctt gcagtgtggc tttattgtca
     1201 tctaaccagc gggttttgat gatgtcttgg ctacgttcat caagggtggc taacgcatag
     1261 gcaagttgag cggttgcctg cccattatgt tgttcgtctt ccaagtcatc ggcgaaattg
     1321 gagctgctat cttcaatata catagaagga gcgtagctgt cttcatcgtc atcaccggca
     1381 ggcaggtcaa aacctaaatc ttgtccggtc attcgagatt ccatttctcg tacttcttcc
     1441 actgaaaccc ctaaatcatc ggcaactttt ttgatctctt cttcattaaa ccacgccaag
     1501 cggtttttat ttttgcgtaa attaaagaat aatttacgtt gagctttggt ggttgccact
     1561 ttcacaattc gccagttttt cagcacatat tcgtgaattt ccgctttcac ccaatgaacc
     1621 gcaaaagaaa ccaaccgcac gccgacattc gggtcaaacc gttttaccgc tttcatcaag
     1681 ccgatattac cctcttggat cagatctgcc agcggtaaac catagcccaa atagccacga
     1741 gcaatatgga tcacaaaccg catatgggaa aggatcagct gttttgccgc ctcaacgtct
     1801 tcatcataat aataacgctc cgccagttct ttttcttgct cggcggtgag aatcggatac
     1861 tgatttgcca ttcggatgta gctatccaaa ttgccctgcg gcacaggcat tgagccggaa
     1921 accaaagccg gatgcattgc ttctaccgat gtttcttctt cgtctatctc atcaacatca
     1981 atcggctcgg catctataat ttcagcatct tcaatctcgt tctcgttaaa ttttttcatt
     2041 ctatttctct tcactctcag caaggtttac attataacag agttaaatct atctggtgtg
     2101 ggatttgtat gattttattt cgagctattc aataccgcta agttccgcca cttgcaaaac
     2161 tttttgcaaa ttcgaccgct ttcctttaga ataccgctac ttttggggct gattttggat
     2221 tcgacgggat tggcgaagcc caaggtgcat gtcgaggtgc ggtaggcctc gtaaacaaac
     2281 cgcaaaataa tagtcgcaaa cgacgaacaa tacgctttag cagcttaata acctgcttta
     2341 aagccttatc tcctcagctt ccgctcgtaa gacgagggca aagataagtc acccaaaacg
     2401 agatcgtgtg gacgccgccg ctttaggatc gaaacactaa attaaataaa gctagtttat
     2461 tcattgcgtg tctgtccgct gtgaataagc gaaattaaag actagactaa acatgtagta
     2521 ctgaaggtag agaaatttcg gacgggggtt caaatccccc cagctccacc aaattataat
     2581 cctatacggt tcaaccgata cctaaaaagc cttgaaaatc aatgtttcaa ggctttttta
     2641 ttgtccgttg cgtacctata cgatacaatc aaatccgaag attttaggag tatatttagg
     2701 agtacgccgg aataccccca aaaattcgta ctcctaaaac cactttagag ccttttcaaa
     2761 taaggcttta tcgcttttag ttttgtactc ccaaacaaaa aatcatactc ctaagattta
     2821 ggctttatac tcctaaaggt ttactatggc tagaaaaatc acaccgttaa acgataccca
     2881 aataagaaaa gcgaaaccgg aagacagccc attaagagac ggtaacggct tactacttgt
     2941 tattacctca aattctaaat tgtggcggtt taggtatgaa agaccgttca caaaaaagcg
     3001 gaatgattta agcattgggg cttaccctga tatttcttta gcacaagcga gagaaattag
     3061 agaagaatat agggcattat tggctcaaaa tatcgaccct caaacccacc gccaacaaca
     3121 ggaacaggca aaggccaacg aaatcagcaa cacttatgaa aatgtagcgt gggcgtggct
     3181 ggaacaccgc aagaccaaga agaatttttc agacaactac caaaaagatg taatcgcctt
     3241 aattaaaaat aacctcttgc cgcactttgg aagaatgcct atcacacaaa taaccgcacc
     3301 aatggcaatc aaggcattca agcaatacca agacgagggc aaactagaga agttaaaacg
     3361 ggcaattcaa aaacataatg aaattatgat gtttgccgta caccgtgaaa tgataccgtt
     3421 taaccctacc gccaatattg cgaaagagtt tgatagccca acggttgaac atttcaaaac
     3481 cattaagccg gaagatttag gcgagttttt atacacctta caaaacgccc aaattcattt
     3541 acagacacgc tatttaattc tgtggcagtt actcacaatg acccgcccga atgaagcggc
     3601 taaggcaaaa tgggcggata tagacgaaaa agccaaactg tggacgatac cggcggaaaa
     3661 tatgaagcga ggcattgagc ataaaatcac actctcacgc caagcaatcg cacttttgcg
     3721 agagattaaa aagctaagtg gcggtaaaac gtatttattc cctagcgtga agaacccaca
     3781 aacgcacgtc aatactcaaa ccgccaacgg tgcgatcaaa cgaatgggct acaagggcaa
     3841 attagtggct cacggcttgc gggccatcgc ctccacttat cttaatgaga aaggctacaa
     3901 tagcgattta atcgaggttg ccttatcgca tttaaacagc gaccgaatca aagccgccta
     3961 tgatagaggc gaacgcctag agcaacgttt taaactgctc caagtgtggg cggattttgt
     4021 agaggaatgt tcgcaaggta gcttaccgca atatcatttg aaaatagcct agtaaatttt
     4081 ccgccaaaaa tgaccgcttg taatgccgtt aggtaaaccg cctgacggct tttttatgcc
     4141 cgtaattccc cttgatgaaa attctgtaaa cttttcga
//
LOCUS       DANLXF010000204         4170 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.204, whole genome shotgun sequence.
ACCESSION   DANLXF010000204 DANLXF010000000
VERSION     DANLXF010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.204"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..1081)
                     /locus_tag="PXD66_002117"
     CDS             complement(<1..1081)
                     /locus_tag="PXD66_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL6320374.1"
                     /translation="MGIMSKSISIFNHKGGIAKTTTAFNVGWSLANQGYQVLLVDLDS
                     QCNLTGLVLGYDQCKEDSDLALFYNNRHHLTMEGIVEALINGQSPDNYLSGNVGQITQ
                     TLNPNLFLLPGHLNVSELDSQISVSLKIAAGVPATRNIPGNLPTLIKKVAEQNNIDYI
                     LYDLSPNVGGLNEVILMSSDYFIVPTSPDFFCWQAVGSLAKNIRKWHNELKAFKELNG
                     FNQSSYAIKNKPQFLGTIQQRYRPRKGQPAKSFEKWIERIRNEVITNFVPVLKTIDCA
                     IDDTAIYAALKSTNSDLEPFDLAHIADFNSLIAISQELRVPVFSLTKEQIKNSGQFGH
                     ALNTMDESKENFDQEFQSLAERIIQL"
     gene            complement(1146..1577)
                     /locus_tag="PXD66_002118"
     CDS             complement(1146..1577)
                     /locus_tag="PXD66_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320375.1"
                     /translation="MKKMAIVALSAFFSMNAFANNNVVYSCTTTDNQTLKVTKEGGNY
                     VYSHGNTTFKNPVKEALKNPASEIAGGSQFTTISLELRNAGKSYIVGHIEADPKSPFE
                     ASVQIQDIKTGNDITSFECRSDKPIRHNFDRKLMRKSGFAA"
     gene            complement(1581..3683)
                     /locus_tag="PXD66_002119"
     CDS             complement(1581..3683)
                     /locus_tag="PXD66_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HDL6320376.1"
                     /translation="MKKAKSLKFLTACIMLTTMSGYANSNTVNVGNSISNFIENTTGY
                     RTGNLGVSGNNALTEYYPVLSDYMHYSIGGGSAISAPASRGRPNQYGIGIGWKSNLTC
                     GNFDIKLSIKNQLNGITEGFKDLYGNIIESATGAVASLPAMIIQRANPQLYDILSNGL
                     YQGKLDFNNFKTSCEAMSNQLADLAMDSKLAKMAGLHRAAEIAETEPDAMAAKKKMDK
                     KDAEKGIPWVGGEQRGGKNQKPMAIVADIVGAGYNLALGRGPLEKGDIPIASCDGMLC
                     TEWKNPSEAAAYASEVIGETFLDACETCEQSSSGAKAGKGIAHFIEQTTIEKATKLEA
                     ALNHLDSLTPEQLNSLSTSTVSVTKGVLEAIAASPDAALLGARLSQELAISKEMEKLL
                     LIRRAMLAGMREPNVIKSSLVEEAERNLKLLDREIEQVKLEMELQRSINGNTALAAIT
                     QQMNEQALAIDDNAMGDGNRRFANLSNQAGANNLNTSGIANSAQNAVGDRYIAIPKSD
                     GSGMSDFYGAYKASPTNIVSNVNVGSYTQIAQMNGNANEQATALIKQFEGYLSHGKWD
                     VNANRAGYGSDTYTTADGMVHRVQKGTYVSREDAERDLARRMPQFMNVARGNVGADVW
                     AKLTPQAQAALTSVSYNYGSLSKLESVQKAAAVSAQSGDMTALANSIRNLQGHNNGVN
                     ARRRNQEADYIINAPKGN"
BASE COUNT         1157 a          824 c          797 g         1392 t
ORIGIN      
        1 tcagttgtat aattcgttct gctaatgatt gaaattcttg gtcaaaattt tctttacttt
       61 catccattgt attcaaagca tgtccaaatt gacctgagtt tttaatttgc tcttttgtta
      121 aggaaaatac gggaactcgt agctcttggc taatagcaat caacgaatta aagtcggcta
      181 tgtgagccaa atcaaatggc tctaagtcag aattcgttga tttaagagca gcataaatag
      241 ctgtatcatc aatagcacaa tcaatagttt ttaataccgg cacaaaattc gtaatgactt
      301 catttctaat tcgctcaatc catttttcaa atgatttggc cggttgccct tttcttggtc
      361 gataacgttg ctgaatagta cctaaaaatt gtggcttatt tttaattgca taagatgatt
      421 ggttaaaacc attaagttct ttaaatgctt ttaactcatt atgccatttg cgaatatttt
      481 tagcgagcga accaactgct tgccagcaga aaaaatcagg tgatgtaggt acaataaagt
      541 aatcactaga cattaggata acttcattta atccacccac atttggacta agatcataga
      601 gtatgtaatc aatattgttt tgttcagcta ctttcttaat taaagtcgga agattgccgg
      661 gtatatttct tgttgctgga acaccggcag caatttttaa tgatacacta atttgcgaat
      721 caagctcgga aacattaagg tgaccaggta acaagaacag atttggattc agggtttgag
      781 tgatttggcc aacatttcca ctaaggtagt tatcagggct ttgtccgttt atcagtgctt
      841 caacaatacc ttccatagtc aaatgatgtc tattattgta aaaaagcgct aaatcactat
      901 cttctttgca ttgatcgtag ccaagtacca gaccggttag gttacattgt gaatccaaat
      961 cgactaacaa tacttgatag ccttgatttg ctaaactcca accaacatta aatgcagttg
     1021 tagttttagc aatacctcct ttatgattaa aaatactgat agacttactc ataataccca
     1081 taattctatg aaataattaa cccaccgaac tcatttgaga atggtgggtt aatgctttgc
     1141 tttagctagg ctgcaaatcc agattttctc atcagtttgc gatcaaagtt gtggcgaata
     1201 ggcttatcgg aacgacattc aaatgatgta atatcatttc ctgttttaat atcctgaatc
     1261 tgaacacttg cttcaaaagg tgatttaggg tctgcttcta tatgacctac aatataagat
     1321 tttcctgcat ttcttaattc gagcgagatt gttgtgaatt gtgaaccacc tgcaatctcc
     1381 gatgccggat ttttgagagc ttcttttaca ggatttttga atgtggtatt accgtgagag
     1441 tacacatagt ttcccccctc tttggtaact tttaacgttt ggttatctgt agttgtgcaa
     1501 gaatacacta cattgttatt tgcaaaagca ttcattgaga aaaatgctga taaagctaca
     1561 attgccattt ttttcatagg ttagtttcct tttggtgcgt tgataatgta atcggcttcc
     1621 tggttacgtc ttcgagcatt aacaccatta ttatgccctt gtaaatttcg gattgagtta
     1681 gctaaagccg tcatatcacc tgattgtgcg gagacggcag ccgctttttg aacactttct
     1741 agtttagata atgacccata gttgtatgat accgacgtta atgctgcttg tgcttgagga
     1801 gtcaatttag cccaaacgtc ggcaccaaca ttaccccttg caacattcat aaattgaggc
     1861 attcttcgtg ctaaatcacg ttcagcatct tcccggctta cataagtgcc tttttgaacc
     1921 cgatgaacca taccatctgc agtagtatag gtgtcagagc catatccggc acggttagca
     1981 tttacatccc atttcccgtg gcttaaatat ccttcaaact gtttgataag agccgttgct
     2041 tgttcattcg cattgccgtt catttgggca atttgggtat agctccctac attcacgtta
     2101 ctgacaatgt ttgttgggga ggctttatat gctccataaa aatcactcat gcctgagccg
     2161 tctgatttgg ggatagcaat atatctatcg ccaacggcat tttgtgcaga attagcaatg
     2221 ccactggtgt tgagattatt tgctccggct tgattgctta aattggcaaa gcgacggttt
     2281 ccatcaccca tcgcattatc atcaatagca agagcttgtt cgttcatctg ttgagtaatt
     2341 gcagctaatg ctgtattacc gttgattgaa cgttgcagtt ccatttcgag tttaacttgc
     2401 tcaatttctc tgtcaagtaa tttgaggttt ctttctgctt cttctactaa ggaagatttt
     2461 ataacatttg gctcacgcat accggctaac attgctcggc gaatcagtag caatttttcc
     2521 atttctttag aaatagctaa ttcttgcgat aaccttgcac ccagcaaggc tgcatccggc
     2581 gaagcagcaa tggcttctaa tacgcctttt gttacactga ccgttgatgt actcagtgaa
     2641 ttgagttgtt ccggcgttaa actatctaag tgattcagtg ccgcttctag ctttgtggct
     2701 ttttcaatag ttgtttgttc aataaaatgg gcgattcctt tcccggcttt agctccactt
     2761 gaactttgtt cacaagtttc acaggcatca aggaatgttt caccaatcac ttcacttgca
     2821 taggcagcag cctcgcttgg atttttccat tccgtacaaa gcataccatc acagcttgca
     2881 ataggtatat ctcctttttc taatggtcct cgtcctaaag cgaggttata tccggctcca
     2941 acaatgtcag caacgatagc cattggtttt tgatttttac caccacgttg ttcgccacct
     3001 acccaaggga tgcctttttc agcatctttt ttatccattt tcttcttggc tgccattgca
     3061 tcaggctctg tttcagcaat ttcagcggct cggtgtaatc cggccatctt tgctaattta
     3121 ctgtccattg ctaaatcagc aagttggtta gacatggctt cgcaactggt tttgaagttg
     3181 ttgaagtcaa gttttccttg atatagacca ttagaaagaa tgtcatacag ttgaggattt
     3241 gctcgttgga taatcatcgc cgggaggctg gcaactgcac cggttgctga ctcaataata
     3301 ttgccataaa gatctttaaa accttcagta ataccattaa gctggttttt aatggaaagt
     3361 ttaatatcaa agttgccaca agttaaattc gatttccagc caatgccaat cccatattga
     3421 tttggtctgc cacgggaagc tggtgcggaa atggcactac ctcctccaat ggagtagtgc
     3481 atataatcag aaaggacagg gtagtattcc gtgagagcgt tattaccaga aacgcctaga
     3541 ttaccagtcc tatagcctgt tgtattttca ataaaattac taatgctatt gccaacatta
     3601 acggtattac tatttgcgta tccgctcatg gttgtgagca ttatacaagc ggttagaaat
     3661 ttgagacttt ttgctttttt catttattta tcctaatagt gctgataatt taatgagtat
     3721 gtgtactttt ccagtccaca tgacctaaaa aaacttgccc ttgtttttta cagcaagaat
     3781 agggcctcca taatgcccag ctgtaattct cagtattgga gaaactatct gaatgggtag
     3841 cccaatcacc actgtgcgga aagactttag ggtaggattc ggaagtaaat accttaactg
     3901 ttacatctga gggtttatta gcaggactag cacttgccat agctgaaagc agtagtagtg
     3961 gtaaatagta aaatgtctta gcttgcataa atgctccaca aatagagtaa ttcacgttta
     4021 ccttaaaaga tgatttggtt agaagaaatc ggaaattgtt ttttggtttt ggagagtttt
     4081 tgttagtata taagttaatg taacaaggga gagtggatgt atgagcaata gaatacctaa
     4141 agcctgttat gcttacccaa aatcgccgaa
//
LOCUS       DANLXF010000205         4155 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.205, whole genome shotgun sequence.
ACCESSION   DANLXF010000205 DANLXF010000000
VERSION     DANLXF010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.205"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            87..2093
                     /gene="tkt"
                     /locus_tag="PXD66_002120"
     CDS             87..2093
                     /gene="tkt"
                     /locus_tag="PXD66_002120"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDL6320377.1"
                     /translation="MTERKVLANAIRFLSMDAVQKANSGHPGAPMGMADIAEVLWRDF
                     LKHNPTNPKWADRDRFVLSNGHGSMLIYSLLHLTGYDLSIEDLKQFRQLHSKTPGHPE
                     YGYAPGVETTTGPLGQGITNAVGIAIAEKTLAAQFNREGHKIVDHYTYAFLGDGCLME
                     GISHEACSLAGTLGLGKLIAFYDDNNISIDGHVDGWFTDDTAQRFESYGWQVIRNVDG
                     HNPEQIKFAIENAQAETERPTLIICKTIIGYGSPNKSNSHDCHGAPLGDAEIQAAREF
                     LGWEHAPFVIPAEIYTEWDAKAKGTLAEKDWNAKFAAYEAAYPELAAEFKRRYAGELP
                     ANWEAESKAFIEKLQANPATIASRKASQNAIEAYAPILPEFLGGSADLASSNLTLWSG
                     SKPIRAEHNVDGNYINYGVREFGMSAIMNGIALHGGFIPYGATFLMFYEYAHNAVRMA
                     ALMKQRALFVYTHDSIGLGEDGPTHQPVEQTASLRQIPNLETWRPADQVESAVAWKAA
                     VERKDGPTALIFTRQNLAQQQRTAEQLANISRGGYILRECCEKGSCPDLILIATGSEV
                     DLAMKAADVLDAEGVKVRVVSMPSTNVFDKQDEAYRESVLPRSVTKRVAIEAQLADFW
                     YKYVGFDGRIVGMNSFGESAPADQLFKLFGFTVENVVAKAKEIL"
     gene            complement(2219..3289)
                     /locus_tag="PXD66_002121"
     CDS             complement(2219..3289)
                     /locus_tag="PXD66_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDL6320378.1"
                     /translation="MNEIIFSLEPQDNARLQSLCGAFDEHLALIEKSFNLIIARTGFN
                     FTIQSEDDNHYSATLVQNSVKLIKQLYNETAPIKGKIKELDLEDIHLAIQESRMLLQN
                     QWQAGSQGEIAIKTKRGVIKPRSEHQQAYLRNILSHDISFGIGPAGTGKTFLAVAAAV
                     ESLERQEIRRILLTRPAVEAGEKLGFLPGDLGQKIEPYLRPLYDALFEMLGFEKAQKL
                     MERGVIEIAPLAYMRGRTLNDAFIILDESQNTTTEQMKMFLTRIGFNSKAVITGDITQ
                     VDLPRSQKSGLKHAMEVLKEVPELSFNFFDSKDIVRHPVVAKIVQAYDAWEAVDEERK
                     EQRKLEKLALEQEKILAQAEML"
     gene            complement(3304..4029)
                     /gene="fadR"
                     /locus_tag="PXD66_002122"
     CDS             complement(3304..4029)
                     /gene="fadR"
                     /locus_tag="PXD66_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HDL6320379.1"
                     /translation="MGHSDILKAQSPAALAEEYIVKSIWNNHFPAGSDLPAERELAER
                     IGVTRTTLREVLQRLAREGWLSIQHGKPTRVNDIWETSGPNIIGTIIKLDKSYLPVVI
                     ANVVSLRTRMAEAYIPEAIYLAPEESAKLFDGLDELENTAAAYAEFDYKLFRQFTFLA
                     NKPVYALILNSFKSMYHQVAKIFFADENNRQLTLSFYRSLLCTCLAKDSEKAAACMAK
                     NRQSSGEIWQKLSANIPESFGQL"
BASE COUNT         1216 a          898 c          826 g         1215 t
ORIGIN      
        1 ttttgagttt ttctagcaaa atagtgtaga atccaattcg atatcctttc cattttatat
       61 aacattctcg attaggagta acacaaatga cagaacgtaa agtgctagcg aatgcaattc
      121 gttttttaag tatggatgct gtacaaaaag ctaactcagg ccacccaggc gcaccaatgg
      181 gtatggctga tattgcagaa gttttatggc gtgatttttt aaaacacaat ccaactaatc
      241 ctaaatgggc agatcgtgac cgttttgttc tgtctaacgg acacggttca atgttaatct
      301 atagcttact tcatttaacc ggctatgatc tctctattga agatttaaaa cagttccgtc
      361 agttacattc taaaacacct ggtcaccctg aatatggtta tgcaccgggt gttgaaacca
      421 ccacaggccc gttaggtcaa ggtattacca atgcggtcgg tatagcgatt gcggaaaaaa
      481 cccttgcagc acaatttaac cgtgaaggtc ataaaattgt cgatcactac acttacgctt
      541 tcttaggcga tggctgttta atggaaggta tctcacacga agcttgctct ctcgccggta
      601 cattaggctt aggcaaactg attgctttct acgatgataa caatatctct atcgacggcc
      661 acgttgatgg ttggttcact gatgataccg cacaacgctt tgagtcttat ggctggcaag
      721 ttatccgtaa tgtcgatggc cataacccag aacaaatcaa atttgcgatt gaaaatgcac
      781 aagccgaaac cgagcgtcca actttaatta tttgtaaaac cattatcggc tatggttcac
      841 caaataaatc aaactctcac gattgccacg gtgcgccatt gggtgatgct gaaatccaag
      901 ccgctcgtga atttttaggt tgggaacatg ccccatttgt gattccagct gaaatttata
      961 cagagtggga tgcaaaagca aaaggtacat tagcagaaaa agattggaac gctaaatttg
     1021 ctgcttatga agcggcatac cctgaactag ccgctgaatt taaacgccgt tacgcaggcg
     1081 aattaccggc taactgggaa gctgaaagca aagcttttat tgaaaaatta caagcaaatc
     1141 cggcaaccat tgcaagccgt aaagcatcac aaaatgcaat cgaagcttac gcgccaatct
     1201 tacctgaatt cctcggcggc tcagctgact tagcaagctc taacttaacc ttatggtctg
     1261 gctcaaaacc gattcgtgcg gaacacaatg tagatggtaa ctacatcaac tacggcgtgc
     1321 gtgaattcgg tatgtctgcg attatgaacg ggattgccct acacggtggt ttcattcctt
     1381 acggtgcaac cttcttaatg ttctacgaat atgcacacaa tgcggtgcgt atggcagcct
     1441 taatgaagca acgtgcctta tttgtttata cacacgactc catcggttta ggtgaagacg
     1501 gcccgaccca ccaaccggta gaacaaactg cttcattacg ccaaattcca aacttagaaa
     1561 cctggcgtcc ggcagaccaa gtagaatctg ctgttgcttg gaaagcggcg gtagaacgta
     1621 aagacggccc gacagcacta atctttaccc gccaaaatct ggctcaacaa caacgtactg
     1681 cggagcaatt agcgaacatc tctcgtggtg gctatatctt gcgtgaatgt tgcgaaaaag
     1741 gcagttgccc ggatttaatc ttaattgcaa ccggttctga agtagattta gcaatgaaag
     1801 ccgctgatgt gttagatgca gaaggcgtga aagtgcgtgt agtttcaatg ccaagcacca
     1861 acgtgtttga caaacaagat gaagcctatc gtgaatcggt attaccacgt tcagtcacta
     1921 aacgtgtggc gattgaagct caattagctg acttctggta taaatatgtc ggctttgatg
     1981 gtcgaattgt aggtatgaat agctttggtg aatccgcacc agcagatcaa ttattcaaac
     2041 tcttcggctt taccgttgaa aacgtggttg ctaaagcaaa agaaatctta taatgaaaca
     2101 ataagataaa gaaaagcggg aattaacttc ccgctttttt gttgaacaac tgttatgata
     2161 agcggtgaaa tttcagcgat tttttgcaaa aaattagcga aatatgaccg cttgtaagct
     2221 ataacatttc agcttgagca agaatttttt cttgctctaa agctaatttt tccagttttc
     2281 gctgttcttt acgctcttca tcaaccgctt cccacgcatc ataggcttgc acaattttgg
     2341 caaccacagg gtggcggaca atatctttgc tgtcaaagaa attaaagctg agttccggca
     2401 cttcttttag cacttccatt gcgtgtttta agcctgattt ttggctacgg ggcaaatcca
     2461 cttgggtaat atcgccggta atcaccgctt tggaattaaa gccaatacgg gttaagaaca
     2521 ttttcatttg ctcggtagtc gtgttttggc tttcatctaa aatgatgaac gcatcgttta
     2581 aagtacgccc tcgcatataa gcaagtgggg caatttcaat cacaccacgt tccatcaact
     2641 tttgagcttt ttcaaagcca agcatttcaa acagggcatc atataacggg cgtaagtaag
     2701 gctcaatttt ttgccctaaa tcgccgggta aaaaaccgag cttttcaccg gcttccaccg
     2761 ccggacgggt aagtaaaata cggcggattt cttggcgttc aagcgattcc accgctgccg
     2821 ctaccgctaa aaaggttttg cctgtgccag caggcccgat accaaaactg atgtcgtggc
     2881 ttaatatatt tcgtaaatag gcttgttgat gttcgctacg aggtttaatc acccctcgct
     2941 tggttttaat cgctatttcg ccttgcgagc cggcttgcca ttggttttgt agtagcattc
     3001 ggctttcctg aatcgccaaa tgtatatctt ctaagtccaa ttccttaatt ttgcctttaa
     3061 tcggggcagt ttcattatac agttgcttaa tcagcttcac actattttga accaaggtag
     3121 cagaatagtg attgtcatct tccgattgaa tcgtaaagtt gaagcctgtt cgagcaataa
     3181 tgagattaaa acttttttca attaaggcta aatgctcatc aaatgcacca caaagcgatt
     3241 gcagacgagc attatcttgc ggttctaagc taaaaataat ttcgttcaaa attctacctt
     3301 ctgttaaagc tgaccaaagc tttcaggaat attagcggat aatttttgcc aaatttcacc
     3361 actagattgg cgatttttag ccatacaagc ggctgctttt tcactatctt ttgcaagaca
     3421 agtacataat aatgaacggt aaaagcttaa cgttaattga cgattatttt catcagcaaa
     3481 gaaaattttt gcgacttgat gatacattga tttaaagcta tttaggatca acgcatacac
     3541 aggcttattg gctaaaaaag taaattgtct gaataactta taatcaaatt cagcataagc
     3601 agctgctgta ttttctaatt catctagtcc atcaaagagt ttagcacttt cttcaggagc
     3661 aaggtaaatc gcttctggaa tataagcctc cgccatacga gtacgtaatg acaccacatt
     3721 ggcaatcacc actggaagat agcttttatc tagcttaata atagtgccaa taatattagg
     3781 ccccgaagtt tcccaaatat cattcactct tgttggcttg ccgtgctgaa tacttaacca
     3841 accttctcgc gctagacgtt gtagtacttc acgtaaagtt gttcttgtta ccccaatgcg
     3901 ctctgctaac tctcgctcgg caggaagatc agagccggca gggaaatgat tattccaaat
     3961 actctttaca atatattctt ccgctaacgc tgccgggctt tgcgctttta aaatatcact
     4021 gtgacccatt aatgcccccc aagcaaaaaa tatgcttatc taaaatctaa ctattcttta
     4081 tttctaatca attattgcga ctaaaaagtt cagtaatact attatccttt aatctaaact
     4141 atattacaat tgtaa
//
LOCUS       DANLXF010000206         4152 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.206, whole genome shotgun sequence.
ACCESSION   DANLXF010000206 DANLXF010000000
VERSION     DANLXF010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.206"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            243..1925
                     /gene="recN"
                     /locus_tag="PXD66_002123"
     CDS             243..1925
                     /gene="recN"
                     /locus_tag="PXD66_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDL6320380.1"
                     /translation="MLTQLTINNFAIVRHLILELNEGMSVITGETGAGKSIAIDALGL
                     CLGYRSESGMIRNGSDKADISATFTMQPHSPAYLWLQEHELLDEDNPQECILRRMINI
                     DGRSKAFVNNRSLPVSQLRELGQYLIHLNGQHAPQLLLKNEYQLEIVDHYATLQPLLM
                     KMAKQYGNWKELNKQVKNFQKQCQENEARKQLLEYQVEELDEFAIKANEFEEMEEKYN
                     ILSSSEELTELSDEITRLLSDDEINANSLIYKALRNAENLAEVDSQYQGILDMLNEAL
                     IQIQEASSEVAQLASKIEQDPELLQELDNRIAQTISLARKHHIQPNELWQYHATLKAE
                     LQELLDFEDNEEQLIQNEKQAYQECITLAEEIYQKRLDATHKLSEQVTKQIKHLAMEN
                     GEFFIHIEHNLEKMSANGADYVEFNLRSNLGQQPQPLAKIASGGELSRISLAVQVLTA
                     NKLSTPTIIFDEVDVGISGSAATTVGKLLRKLGEKCQVLCVTHLPQVASYGNQQFNVQ
                     KYVENNQTETQMTLLTEAERIHALARLLGGSEITETVLANAKEMLNLAKNNE"
     gene            complement(1972..2047)
                     /locus_tag="PXD66_002124"
     tRNA            complement(1972..2047)
                     /locus_tag="PXD66_002124"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2012..2014),aa:Ala,seq:ggc)
     gene            complement(2127..3566)
                     /gene="gltX"
                     /locus_tag="PXD66_002125"
     CDS             complement(2127..3566)
                     /gene="gltX"
                     /locus_tag="PXD66_002125"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDL6320381.1"
                     /translation="MKIETLFPLDPNVKVRTRFAPSPTGYLHVGGARTALYSWLYAKH
                     NDGEFVLRIEDTDLERSTPEATAAILEGMEWLDLAWEHGPYFQTKRFDRYNQVIDQML
                     EAGTAYRCYCSKERLDEVRAEQEANKEKAKYDQHCLHNPQNDPSQPHVVRFKNPTEGS
                     VVFDDAVRGRIEIANSELDDLIIRRTDGSPTYNFCVVVDDWDMGITHVVRGEDHINNT
                     PRQINILKALGAPIPTYAHVSMINGDDGQKLSKRHGAVSVMQYRDDGYLPEALINYLV
                     RLGWGHGDQEIFSRDEMIELFDIHSVSKSASAFNTEKLQWLNQHYIRSLAPSYVAKHL
                     EWHMQTQGIDFTSGPKLEEIIPVLGERCKTLKELAESSRYFYQEFKAYDEKAAAKNFK
                     AEAVAPLAKLLEKLTACNDWTVENIHEAMNQTAAELEIGMGKVGMPFRLAVTGSGQSP
                     SMDITAKLVGKERTLARLQKAIEFIQANS"
     gene            3741..3816
                     /locus_tag="PXD66_002126"
     tRNA            3741..3816
                     /locus_tag="PXD66_002126"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3774..3776,aa:Val,seq:tac)
     gene            3865..3940
                     /locus_tag="PXD66_002127"
     tRNA            3865..3940
                     /locus_tag="PXD66_002127"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3898..3900,aa:Val,seq:tac)
     gene            3993..4068
                     /locus_tag="PXD66_002128"
     tRNA            3993..4068
                     /locus_tag="PXD66_002128"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4026..4028,aa:Val,seq:tac)
BASE COUNT         1248 a          857 c          871 g         1176 t
ORIGIN      
        1 gtttgttacc tttgaagata aagcagaagt cccacaaggt taccaagtac aacaagaaat
       61 ttttatttag tctaaatggc ttaaaagacg aacagaaatg ttcgtctttt attttgcaaa
      121 ttttttctaa tatttaaccg cttgcaaccc gcctcattag cgattttttc gcccttatct
      181 atttactgtt taaaaaaaca gttgtactat atacccatac agttaatctt gtgtggtgta
      241 ttatgcttac tcaacttact attaataatt ttgcgattgt tcgccatctt attcttgaat
      301 taaatgaagg tatgtctgtt attaccggtg aaaccggtgc cgggaaatca atcgctattg
      361 atgccttagg gctatgcttg ggttatcgtt ctgaaagtgg gatgattaga aacggttcgg
      421 acaaagcaga tataagtgca acctttacta tgcagccaca tagccctgct tatttatggc
      481 tacaagagca tgaactgctt gatgaagata atcctcaaga gtgtatctta cgccgtatga
      541 ttaatattga tggacgctca aaagcctttg taaataaccg atcattacct gtttcacaac
      601 ttcgagagtt ggggcaatat cttattcatt taaatgggca acacgcacca caattactcc
      661 taaaaaacga atatcaactt gaaatagtcg atcattatgc cacgttacag cctctattga
      721 tgaagatggc aaagcaatat gggaattgga aagaactgaa taagcaggta aaaaatttcc
      781 aaaaacagtg ccaagaaaat gaagctcgca aacagctatt agaatatcaa gttgaggaat
      841 tagatgaatt tgcaattaaa gcaaacgaat ttgaagaaat ggaagagaaa tacaatatct
      901 tgtcttcttc cgaggagctt actgagcttt cagatgaaat cacacgctta ttaagtgatg
      961 atgaaatcaa tgcaaacagt ctgatttata aagcgttacg caatgcagaa aacttggctg
     1021 aggttgatag ccaatatcag ggcattttag atatgcttaa tgaggccctg attcagattc
     1081 aagaagcaag ctccgaagtg gctcaattag ccagtaaaat tgaacaagat ccggaattac
     1141 tacaggagct ggataataga attgcacaaa ctatatcgtt agcacgcaaa caccatattc
     1201 aaccgaatga attatggcaa tatcacgcga cattaaaagc agaactacag gaattactgg
     1261 attttgaaga taatgaagaa caactcattc aaaatgaaaa acaagcttac caagagtgta
     1321 taacattagc agaagaaatt taccaaaaac gtctagatgc cacacataag ctctctgaac
     1381 aagtaacaaa acaaattaaa cacttagcaa tggaaaacgg tgagttcttt attcatatag
     1441 aacataatct tgaaaaaatg tctgcaaatg gtgctgacta tgtcgagttt aatttacgca
     1501 gcaatttagg acaacaaccc caaccactgg caaaaattgc ttctggtggt gagctatctc
     1561 gcatttctct ggcagtacaa gtgcttaccg caaataaact ttctacgcca actattattt
     1621 ttgatgaagt tgatgttggg attagtggct ctgctgccac tacagtcgga aagctactgc
     1681 gtaagctagg cgaaaaatgc caagtattat gtgtaaccca tttacctcaa gtggcaagtt
     1741 acggcaacca gcagtttaat gtacaaaaat atgtagaaaa taatcaaact gaaacgcaaa
     1801 tgacattact cacagaagca gagcgtattc acgccttggc tcgactatta ggcggaagcg
     1861 aaatcaccga aacggtttta gctaatgcaa aagaaatgct aaatttagcg aaaaataacg
     1921 aatagccaaa aagaaaaatc gcatcaatga atattgatgc gattatgaat ttggtggagg
     1981 taaacgggat cgaaccgatg acctcttgca tgccatgcaa gcgctctccc aactgagcta
     2041 taccccctta atacaagcgg aataatatgc tttccgcttg aagatgtcaa tggctttaat
     2101 caagcaaggt gttgattgat taaaaactag ctattcgcct gaataaactc aatcgctttt
     2161 tgtaagcgag ctaaggttct ttctttaccg actaatttgg cggtgatatc cattgacggc
     2221 gattgcccac ttcctgttac cgctaaacgg aacggcatac cgactttgcc cataccaatt
     2281 tccagctcgg cagcggtttg gttcattgcc tcgtggatat tttccaccgt ccagtcattg
     2341 caagcggtca atttttccaa taattttgca agtggagcaa cggcttcggc tttgaagttt
     2401 ttagcagcag ctttttcgtc gtaagcctta aattcttgat agaaatagcg gctgctttcg
     2461 gcaagctctt ttaaggtttt gcaacgttcg cccagtacag ggataatttc ttccaatttt
     2521 ggaccgcttg tgaaatcaat gccttgagtt tgcatatgcc attcaagatg ttttgccaca
     2581 taacttgggg caagactgcg gatatagtgt tggttcaacc attgtaattt ttcagtgtta
     2641 aatgcacttg ccgatttact gactgaatga atgtcgaaca gctcgatcat ttcgtcacga
     2701 gagaaaatct cttggtcgcc gtgtccccag cctaaacgca caaggtagtt aattaaggct
     2761 tccggtagat agccgtcatc acgatattgc attacgctca ccgcaccgtg gcgtttggag
     2821 agtttttgcc cgtcatcgcc gttgatcatt gaaacgtggg cgtaggttgg gattggtgct
     2881 cctaatgctt tcaaaatgtt gatttggcgt ggtgtattgt tgatatggtc ttcaccccgc
     2941 acaacgtgag taatgcccat atcccagtcg tccaccacca cacagaaatt gtaggtcggc
     3001 gagccgtcag tacggcgaat aatcaagtca tctaattcgc tattggcgat ttcaattcgc
     3061 ccacgcaccg catcgtcaaa tactaccgag ccttcggtcg ggtttttgaa acgcacaaca
     3121 tgtggttggc tcgggtcgtt ttgcggattg tgtaagcagt gctgatcgta tttcgctttc
     3181 tctttatttg cttcttgctc tgcacgtact tcgtctaaac gttctttcga gcagtagcaa
     3241 cggtatgccg tgccggcttc taacatttgg tcgatcactt ggttatagcg atcgaaacgc
     3301 ttggtttgaa agtatgggcc gtgttcccac gccaaatcca gccattccat accctctaaa
     3361 atagcggcgg ttgcctcagg ggttgagcgt tcaagatcgg tatcttcaat acgtaacaca
     3421 aattcaccat cattatgctt agcatataac caagagtaga gcgcggtcct cgccccacct
     3481 acgtgcaagt agccagttgg gctaggtgca aaacgggtac ggacttttac attcggatct
     3541 aaggggaaaa gggtttcaat tttcatttta tattgcctaa ttgatgattc ttaagaaaaa
     3601 cttgtctatt ttactacgct tgagggtgaa tcatagtaaa aaattaaatt tgcggggtaa
     3661 aaaatcagca aacggggtaa aaagaacaaa aagggtttga cgaaacagca aaaatctcta
     3721 taattcactt ccacaacagc gggcgattag ctcagttggg agagcacctc ccttacaagg
     3781 agggggtcac tggttcgaga ccggtatcgc ccaccacttc aacttaagtg tttcgttgtt
     3841 gtatcacaat ctaattttga aaacgggcga ttagctcagt tgggagagca cctcccttac
     3901 aaggaggggg tcactggttc gagaccggta tcgcccacca cttcaactta agtgtttcgt
     3961 tttcaaattc cctttgattt taagtctcaa gtgggcgatt agctcagttg ggagagcacc
     4021 tcccttacaa ggagggggtc actggttcga gaccggtatc gcccaccact taagactaaa
     4081 atcataaact tcaaatggcg atttttcgta tctgaagggc gattagctca gttgggagag
     4141 cacctccctt ac
//
LOCUS       DANLXF010000207         4138 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.207, whole genome shotgun sequence.
ACCESSION   DANLXF010000207 DANLXF010000000
VERSION     DANLXF010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.207"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(47..466)
                     /locus_tag="PXD66_002129"
     CDS             complement(47..466)
                     /locus_tag="PXD66_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320382.1"
                     /translation="MLRLLSFCFIFLLSACTVLPQIKQLPPQVESVKSFKVENNGQQS
                     LLLIQFYENKWRWIQTDPLGAPIARVWLTKKGWEPDGFVMPNKQAQLLFTALVNALNP
                     HSPPFYLDKGWKIEREKSQFTIWLPDSSKWKVEILGE"
     gene            complement(528..1217)
                     /gene="rplA"
                     /locus_tag="PXD66_002130"
     CDS             complement(528..1217)
                     /gene="rplA"
                     /locus_tag="PXD66_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDL6320383.1"
                     /translation="MAKLTKKMKAIKAGVDSTKAYDINEAVEVLKKFATAKFVESVDV
                     AVNLGIDSRKSDQNVRGATVLPHGTGRTARVAVFTQGANAEAAKAAGADLVGMEDLAE
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKSGQ
                     IRYRNDKNGIIHTTIGKADFSAEQLKDNLNALLAALTKAKPTTAKGIFIKKVSISTTM
                     GAGVAVDQASL"
     gene            complement(1222..1650)
                     /gene="rplK"
                     /locus_tag="PXD66_002131"
     CDS             complement(1222..1650)
                     /gene="rplK"
                     /locus_tag="PXD66_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDL6320384.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     RTESLEKGLPIPVVITVYADKSFTFVTKTPPAAVLLKKAVGIKSGSGKPNKDKVGTVT
                     QEQIRQIAETKAADMTGATIETKMKSIAGTARSMGLIVEE"
     gene            complement(1883..2446)
                     /gene="nusG"
                     /locus_tag="PXD66_002132"
     CDS             complement(1883..2446)
                     /gene="nusG"
                     /locus_tag="PXD66_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDL6320385.1"
                     /translation="MSEVEVKENGSKRWYVLQAFSGFENRVAVTLREYIKLHKMEEQF
                     GEVLVPTEEVVENVGGKRRKTERKFFPGYVLVEMEMNDDTWHLVKSVPRVMGFIGGTA
                     DRPAPITKREADRILNRVQETAEKPRHRKEFQPGESILVTEGPFADFSGTVEEVDYEK
                     GRLKVSVSIFGRATPVELEFSQVEKQS"
     gene            complement(2448..2861)
                     /gene="secE"
                     /locus_tag="PXD66_002133"
     CDS             complement(2448..2861)
                     /gene="secE"
                     /locus_tag="PXD66_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDL6320386.1"
                     /translation="MATEIKTKTPEQVEQKGGKSKGANTALWLVAIVFLAVAAVGNAY
                     FSSHFSLIVRVLLLVVLLVGAVALAAITNQGQKAIHFIKESRTELRKIIWPTRPEATQ
                     TTLIVLAMCVVVSLVLWGIDSIIVALITFVTSLRF"
     gene            complement(2974..3996)
                     /locus_tag="PXD66_002134"
     CDS             complement(2974..3996)
                     /locus_tag="PXD66_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL6320387.1"
                     /translation="MANGTSRNRRSSGRVTLADVAKVVGVGQMTVSRALRTPEMVSEP
                     LRKRIEEAIIELGYIPHFSARQLASAESHNLVIVTTSISSTENTLILSALRKSVADSA
                     LQMVILIADQESWFQELINHAPQAIVFLNLDCPPNVREWIKNSNIPTVEIGAIKKEPI
                     HLSVGIDSHEAMEKMIHFLVKKGYREIGLLSAKQDLSIFKQYLESWHTTLFAHHISPL
                     KIHHVVEDISFSAGATLLTEALLNWEAIDALVFLSDELACGALYEALRRHMSIPKDLA
                     IAGLGGLEVGSISYPKLTTIAIPYEQMGKIAGKQLLTLLSGKTLSAEEMIVKLPTKLV
                     IRDSAA"
BASE COUNT         1181 a          892 c          730 g         1335 t
ORIGIN      
        1 aaaattcata ttcaagtgat tcaagcatag aggttcaagt tttcgcttat tcccccaata
       61 tttccacttt ccatttactg ctatctggta gccaaatcgt aaattgggat ttttccctct
      121 caattttcca acctttatct aaataaaaag gtggagaatg agggtttaat gcattgacta
      181 aagctgtaaa taatagctgt gcctgcttat ttggcattac aaagccatct ggctcccaac
      241 cttttttagt aagccatact ctggcaatag gagcacctaa aggatcagtt tgaatccaac
      301 gccatttatt ttcataaaat tgaattagaa gcaaactttg ctggccatta ttttcaactt
      361 taaaggattt tacagattca acctgtggcg gtaattgctt aatttgaggt aatacagtac
      421 aagcactaag caaaaaaata aagcaaaaag ataataatct taacattcgt acctaaagaa
      481 aaagaccgta gagtattaac actacggtct tttgattcta ttaaaaatta aagtgatgct
      541 tgatcaaccg caacaccagc acccattgta gtagagatgc ttactttctt gatgaagata
      601 ccttttgctg ttgttggttt tgcttttgtt aaagcagcta acaatgcgtt taagttatct
      661 tttaattgtt cagctgagaa gtctgcttta ccgatagtag tgtggataat accgtttttg
      721 tcgttacggt agcggatctg accagatttc gcatttttaa ccgcttctgc aacgtttggt
      781 gttactgtac ctactttcgg gtttggcatt aagccgcgtg gacctaatac ttgacctaat
      841 tgacctacaa cacgcattgc atctggagaa gcgataacaa catcaaagtt catttcgcct
      901 ttcttgattt gctctgcaag atcttccata cccactaagt cagcacccgc tgcttttgct
      961 gcttctgcgt ttgcaccttg tgtaaacaca gcaacacgag cggtacggcc tgtaccgtgt
     1021 ggtaatacgg ttgcaccacg cacgttttgg tctgatttac gagaatcgat acctaagttt
     1081 acagcaacgt ccacgctctc aacgaattta gcggttgcga atttttttaa cacttcaact
     1141 gcttcgttga tgtcataagc tttagtagaa tctacgccag ctttaattgc tttcattttt
     1201 ttagtcaatt tagccattgt attattcctc tacgattaag cccattgagc gagctgtacc
     1261 agcgattgat ttcattttgg tttcgatagt cgcaccagtc atatctgctg ctttagtttc
     1321 agcgatttga cggatttgct cttgagttac tgtacccact ttatctttgt tcggtttacc
     1381 agaaccagat ttgataccta ccgctttttt cagtagcact gccgccggtg gagttttggt
     1441 aacgaatgtg aaagatttat cagcgtaaac tgtgataaca accggaatcg gtaaaccttt
     1501 ctctaagctc tcagtacgag cgttgaatgc tttacagaat tccataatgt taacaccttg
     1561 ttgacctaat gcaggaccaa ccggtggtga tgggttagcc atacccgctg caacttgcag
     1621 tttaacgtag gcttggactt tttttgccat tttaattttc ctctaaatgg gtgataacgc
     1681 tgaaaatcag ctcccctgtt acataaaaaa tacgagtcag caggctggct cgtaataggt
     1741 cgcaaatttt aaagaatttg cgactatttt tcaagcaaaa ctttcgcttt gttgaataaa
     1801 tcttgttaca agcggtcttt tttccgctga attttgcaaa aaattgagaa aaataaccgc
     1861 ttgtgtgtga aattaattga aattagctct gcttttccac ttgactgaat tctaattcta
     1921 ccggtgtcgc acgaccaaaa atcgacaccg atacttttaa gcgacctttt tcgtaatcca
     1981 cttcttcgac cgtaccggag aaatctgcaa acggaccttc tgtaaccaaa atgctttcgc
     2041 ccggttggaa ttcttttctg tggcgtggtt tttccgctgt ttcttgtaca cggtttagaa
     2101 tgcggtctgc ttcacgtttg gtaattggtg cggggcgatc tgccgtacca ccaataaagc
     2161 ccattacacg aggcacactt ttcactaaat gccaagtatc gtcattcatt tccatttcaa
     2221 ccaatacata acctgggaag aatttacgct cggttttacg gcgtttaccg cccacatttt
     2281 caacgacttc ttcggttggc actaatactt caccaaactg ctcttccatt ttgtgaagct
     2341 taatgtattc acgcaatgtt actgccacac ggttttcaaa gcctgaaaac gcttgtaata
     2401 cataccagcg ttttgagcca ttttctttca cttctacttc actcatttta gaaccttaaa
     2461 cttgttacaa atgtaattaa tgcgacgatg attgagtcaa taccccataa aaccaaggaa
     2521 actactacgc acattgccaa tacaatcaag gtggtttgtg ttgcttcagg gcgtgtcggc
     2581 cagataattt tgcgtagttc ggttcgagac tctttaataa aatgaattgc cttttgcccc
     2641 tgatttgtaa ttgccgctag tgcaacagca ccaactagca acaccaccaa taaaagcact
     2701 cgaacaatta atgaaaaatg agatgaaaaa taggcattac ctaccgcagc aactgctaaa
     2761 aatacaatcg caaccagcca taatgctgta tttgcacctt tgcttttccc ccctttttgt
     2821 tcgacttgct cgggggtttt tgttttaatc tcagtagcca tatataacct aaaaaatgaa
     2881 taaaaaaatc agaaattatt ttaaagcgtt aaattctatc attttctaca agacttgcca
     2941 cgatctttta tagattttta tgcaaaatca atattatgcg gcgctatcac gaatcactag
     3001 cttagtcggt aatttaacaa tcatctcttc tgctgataag gttttaccac tcaaaagagt
     3061 aagtagttgt ttacctgcta tttttcccat ttgctcgtaa gggatcgcaa tggttgtcaa
     3121 tttagggtag gaaatactcc ccacttctaa accacctaaa ccggcaatcg caagatcttt
     3181 tggaatgctc atatgtcggc gtaatgcttc atataatgct ccgcaagcca attcatcgga
     3241 taaaaaaacc aatgcatcta ttgcttccca atttagaagg gcttcagtca acaaagttgc
     3301 tcctgccgaa aacgaaatat cctctactac gtggtggatt tttaaaggag aaatatgatg
     3361 tgcaaataaa gtcgtatgcc aactttctaa atattgtttg aagatggata aatcttgctt
     3421 cgccgataac aatccaatct ctcgatagcc ttttttcacc aaaaaatgga tcattttctc
     3481 cattgcttcg tggctatcaa ttccaacact gagatgaatc ggttcttttt ttatcgcacc
     3541 tatttctacg gtagggatat tgctgttttt aatccactca cggacattag gagggcaatc
     3601 taaattcaaa aacactatcg cttgaggagc atgattaatc agttcttgaa accaagattc
     3661 ttgatcagcg atcaaaatca ccatttgcaa tgcggaatcc gccacgcttt tgcgtaatgc
     3721 cgataaaatt aaggtatttt ccgttgagct aatcgacgtc gtcacaatga ccaaattatg
     3781 tgattctgca gaagctaatt gccgagcaga aaaatgcgga atatatccta gttcaatgat
     3841 tgcctcttca atccgtttac gtagtggctc cgacaccatt tctggtgtac gcaaagcacg
     3901 agataccgtc atctgcccaa caccaacaac tttggctaca tcagctaaag tgactctgcc
     3961 cgaagatctt cgatttctac ttgttccatt tgccatacta ttttcctaat tgttccatat
     4021 tatagggcta aatccttaat tttcctattt agaaagaaca gagcaaaatg ccaactgatt
     4081 attttttatc taattaaata ctaaaaatgt taacgctatc acatttttaa tattttct
//
LOCUS       DANLXF010000208         4126 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.208, whole genome shotgun sequence.
ACCESSION   DANLXF010000208 DANLXF010000000
VERSION     DANLXF010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.208"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(1..2486)
                     /locus_tag="PXD66_002135"
                     /pseudo
     CDS             complement(1..2486)
                     /locus_tag="PXD66_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616807.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
     gene            complement(2581..4017)
                     /gene="glgA"
                     /locus_tag="PXD66_002136"
     CDS             complement(2581..4017)
                     /gene="glgA"
                     /locus_tag="PXD66_002136"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HDL6320388.1"
                     /translation="MKVLHICSEMYPLIKTGGLADVMGALPFAQQQAGNDVRVLIPYY
                     PKVAQTLGETVEVATIGTFAGTVSVRFAYFNGLGVYVIDAPHLFMRDGNPYHDSNYQD
                     YPDNYKRFALLGYIGAQLAQGLDHWWGTAQVLHAHDWQAGLACAYLKSWGSNVKTLFT
                     IHNIAYIGRFNAFHLAELGLPWEFFHAEGLEFYGEISYLKAGLYFADRITTVSPTYAR
                     EITEEIAAAGLHGLLATRQYQGRLRGILNGVDAEVWNPETDTHIVVNYRANYMHGKAK
                     NKAELQRIFGLPEDKEALLMVMVTRLTEQKGADFLLANIDTLMQHKVQIIVLGSGSPD
                     LENALRYFQHKYPNQIGVKIGYDEALSHQIIAGGDLILVPSRFEPCGLTQLYGLKYGT
                     LPLVRRTGGLADTVVDSHKESIENRSATGFVFDYPDAPAFLEAAMRAISLWQKQKLWS
                     SVRQNALAQDFGWAKVAEQYQNLYHEII"
BASE COUNT         1169 a          970 c          804 g         1183 t
ORIGIN      
        1 cagtttattt gaactttcaa taatactgcc cattgtagaa tcgcctttta attgttcttc
       61 gctcatcggt tcgatatgcc aaatattttt cgcataatcc atcaccgagc ggtctgagga
      121 gaaatagccc atattggcaa tattgatgat ggctgaacgt gtccaagctt tcttgttacg
      181 ataggcttga gccacttttt cttgagcctc cacatagcta cgataatcgg cacaggcttg
      241 gtagtagtca cctgaattta agatcaaatc ttgatagcga tacggatctt ccggtgagaa
      301 tttaccgttt agaatttgtg aaattgcctc attcaattcg ctgtcttttt cgtagtagtg
      361 atacggtgag taaccgttgt ggcgaagctc ttccacctgc tctacttcat taccgaaaat
      421 aaagatgttc tctttaccaa cgtgatctaa aatttcgaca ttcgcaccgt ctaatgtacc
      481 aatggttaaa gcaccattga gggcaaattt catattagag gttccggagg cctctgtacc
      541 cgctaaggaa atttgctctg aaacatcagc tgccggaata atcatttgtg ctaatgaaac
      601 accgtagttc ggaataaaca ccaccttaat taaatcatta atgcgagggt cattattcac
      661 cacatttgcc acatcattaa tcaaacgaat cacttttttc gccgcataat aagcacttgc
      721 cgctttccct gcgaagataa acacacgagg tgtccaatca gcagtcgggt ttttcaagat
      781 gcggttataa agcgtgatga tatgcaacac gttcatttgc tggcgtttat acttgtgaat
      841 acgcttaact tgtacatcaa aaatcgcttc aggattaagt ttaattccct gagtttcttc
      901 gacatatttg gctaagcgac gtttgttttc tactttcacc gctgccacct gtgcttgcac
      961 atcagcatca tcaacaaaca cgttgaattt ttccaattca cttaaatcag tcagccactt
     1021 attgccgata catttatcta aaatattagc aagccctggg ttggcaattt tgatccagcg
     1081 gcgaggtgtc accccattag tcacattagt aaaacgattt gggtaaatac gggcaaaatc
     1141 ggcaaaaata gattcaacca ttaaatccga gtgaattttc gccacaccat ttactttcgc
     1201 tgaggcgact accgcaagcc acgccatacg cacacggcga tcgccttgtt catcaattaa
     1261 ggaaacacga gaaatcaaat cgtgatcttc cgggaattgg ctacgcactt cctctaagaa
     1321 atattcgtta atgtcgaaaa taatctctaa atggcgtggt agcacacggg ctaacatttc
     1381 taccggccaa gtttctaacg cttcactcat caaagtgtga ttggtgtaga agaaaatttt
     1441 acgagtcatc gcccacgctt gttcccagcc atagcctttt tcgtcaatta aaatacgcat
     1501 taactcaggc accgcaaggg ttgggtgtgt gtcatttaag tgagttgcca ctttgtcggc
     1561 taaattcaca caagagccga actccgcttc gtggcggcgg ataatatctt gtacagatgc
     1621 cgagcaaagg aaatactctt ggcgtaagcg taattcacga ccgttatagg tagaatcgtc
     1681 cggataaagc acacgagaga cgttttccga gctgttttgt ttaaccattg ctgcaaaata
     1741 atcgccacgg ttaaaatcag ataagccaaa ccctctaccc gatgcgtgtg cagaccataa
     1801 acgtaagctg ttagttgagg tggtctcaaa gcccgggatc agttggtcgt gagcttgagc
     1861 ggtaatttct tcttccggct gccatactac ttttttgccc tcacgccaag ttttaccgcc
     1921 aaagcgtacc gggaaacgct tacttgaacg tagatatggc cacacaaatt cattctctaa
     1981 ccattcatca ggttgctcaa tttgctcccc gttgcggatt tcttggcgga acataccgta
     2041 ttcatattga ataccgtagc cgattgccgg aattttcatt gtggcaaggc tgtccatata
     2101 gcaggcggct aaacgcccta agccaccgtt acctaagccc ggacgccctc ttcattgata
     2161 atctcttcca gatcttgccc taactctttt aacgcagcgc caataagctc atacacacct
     2221 tcagaaatca ttgcgttact gaacgtacgc cccattaaaa attccattga aaggtaataa
     2281 acacggcggc taccatttgc aacctggcta cggcgggttt gtaaccaatc ttcggtagat
     2341 aaatcacgca ctacacgtaa ggttgcattg agccagttac gcttactggc atcacggggt
     2401 tcgaccccta aagcgaacac cattttgtgc aaaatcgcat ttttaacatt tttgatcatt
     2461 tctgggctta atgactgcct gctcattgag taaattctcc taatttttaa tattgcaagc
     2521 ggtcgttttt acaaaaaaat ccgcaaattc gaccgcttga aatgatgttg tgaatacaaa
     2581 ttaaataatt tcgtggtata aattttggta ctgttctgcc acttttgccc aaccaaaatc
     2641 ttgagcaagg gcattctggc gaacgctcga ccacaatttt tgtttctgcc ataagctgat
     2701 tgcccgcata gctgcctcta aaaacgcagg tgcatctggg taatcaaata caaatccggt
     2761 ggcactgcgg ttttcaatgc tctctttatg gctatctacc accgtatcag ctaagccacc
     2821 ggtacggcga accagcggta aagtgccata tttcagccca taaagttgag ttaatccgca
     2881 cggctcaaaa cggcttggta ctagaatcag atcaccacct gcaataattt ggtgcgataa
     2941 agcctcgtca taaccgattt tcaccccaat ttggttcggg tatttgtgtt ggaaataacg
     3001 tagcgcattt tctaaatctg gcgatccact tcccaataca ataatctgta ccttatgttg
     3061 cattaaggta tctatattgg cgagtaagaa atctgccccc ttctgctcag ttaaacgagt
     3121 caccattacc atcaataatg cttctttgtc ttctggcaaa ccaaaaattc gttggagttc
     3181 cgctttattt ttggctttac cgtgcatata gttcgcacga taattcacca caatatgtgt
     3241 atccgtttcc ggattccaca cctcagcatc gaccccgttt aaaatgccac gcaatctacc
     3301 ttgatattga cgggttgcca gtaaaccatg caagcctgca gcggcaattt cctcagtaat
     3361 ttcacgagca taggtcgggc taacggtagt aatgcgatca gcaaaataca aacctgcttt
     3421 gaggtaagaa atttcaccgt aaaattctaa gccttcggcg tggaaaaatt cccaaggtaa
     3481 gccaagctct gctaaatgga aggcattaaa gcgcccgata taggcaatat tatgaatagt
     3541 gaagagcgtt tttacattgc ttccccaact tttaagatat gcacaagcaa gccctgcttg
     3601 ccaatcgtgt gcgtgtaaaa cttgagcggt tccccaccaa tgatccaaac cttgggctaa
     3661 ttgcgcccca atgtaaccta ataaagcaaa gcgtttatag ttatccggat agtcttgata
     3721 attgctgtcg tggtaagggt taccatcacg cataaataaa tgaggcgcat cgataacgta
     3781 aacgcctaag ccattaaaat aagcaaatcg aacagagact gttccggcaa aagtgccgat
     3841 agtagcaact tctaccgttt cccctaaagt ttgcgcgact ttcgggtaat aaggaattaa
     3901 cactctgaca tcattaccag cttgctgttg cgcaaatggc aatgccccca ttacatcagc
     3961 taaaccgcct gttttaatta aagggtacat ttcagagcaa atatgtaaaa ctttcatttt
     4021 taaccgtttt ctatgtgtaa aatagttgaa aaataagacc gcttgaagcg ctttaatcat
     4081 aacaatactt cgagcttgcg acctcaaatg acaaactcga agcatt
//
LOCUS       DANLXF010000209         4126 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.209, whole genome shotgun sequence.
ACCESSION   DANLXF010000209 DANLXF010000000
VERSION     DANLXF010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.209"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..271
                     /locus_tag="PXD66_002137"
     CDS             <1..271
                     /locus_tag="PXD66_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320389.1"
                     /translation="NGGDEIEKISKNNKTQFSSTIRENQINFIRALLYMDYGIETPQE
                     VKNAINSGELGRKIEKFRRENPNIENDKNFKFPTSVTLSNWYSKA"
     gene            349..600
                     /locus_tag="PXD66_002138"
     CDS             349..600
                     /locus_tag="PXD66_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL6320390.1"
                     /translation="MQLQKTISTFTQPNHRGIYVMTYSEQSYLNVKDVCEMLQLNRST
                     IYRQIKQGIFPKPTKIGKSSRWKFADIKAFLEPKPQEAN"
     gene            600..980
                     /locus_tag="PXD66_002139"
     CDS             600..980
                     /locus_tag="PXD66_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL6320391.1"
                     /translation="MAGLSIICPFCGNTARIRGSSRPSLLSYQAQLTCSNCGQLKADF
                     VGQLINIKRAVFIECDEANQWDKPENELIKEGKIQGKDNAQRLKELRGSQQDLFAKVP
                     GQLPPEKITPAEKIAKRANLSKTH"
     gene            1291..2352
                     /locus_tag="PXD66_002140"
     CDS             1291..2352
                     /locus_tag="PXD66_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HDL6320392.1"
                     /translation="MKDLSPNQKQYTPFSAIMQKNLMDLLTKSARSPYSFSTVAKSTA
                     GRENPELHNTANSSTPLTRAIFIRSLHTPKERRNKACSSMVACSGQGSPLAVFGSPFV
                     AVFSPRYTLSPCTVRSVADSPLKLTNGLQKMNPSQITGKNRLNSFLFNNNQNDYSLLF
                     TNAKQSTPISAKISKNQDLCLTTLNRSPYASRAFAKSKAECGNSKEHQANNSTPLTRA
                     FFVRSSRTPKECQNKACSSMVACSGQGFALDCLPDVAVFHPVTRYRPTLWKMLAIAPK
                     NQHQELSKMKAFAYSFLCAIRTPETDTYQETVVRIISDSEQNARFQLTADYRLLLPRP
                     VAKIRLNSTAYTVKGGIYA"
     gene            2345..2509
                     /locus_tag="PXD66_002141"
     CDS             2345..2509
                     /locus_tag="PXD66_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320393.1"
                     /translation="MLNYSDIEQIRYFMDQAEEALDNNEEFKAVAELNTAQQFILALL
                     MNYEQGGENE"
     gene            2502..3137
                     /locus_tag="PXD66_002142"
     CDS             2502..3137
                     /locus_tag="PXD66_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320394.1"
                     /translation="MNNTTGQATQRNWQEIIANLQRLSESGESTIEIAKRYEAFSKTA
                     EQIQALVNNNGSEAEKEAFFAPVERFYLGLLETETRLKDAIAKDEENAQKLKEQKAAR
                     EAEQKRWDNLPFYAKDKFSDLICIESRETLEDKFFAISCMFDALKAVPNLLEIGLREE
                     GKDSVIDRLAEMSKLYLLFVDEQFSLLANEIQSGKYGKLDLNGSQNKGDLC"
     gene            3131..3349
                     /locus_tag="PXD66_002143"
     CDS             3131..3349
                     /locus_tag="PXD66_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320395.1"
                     /translation="MLTHRITLPDGTEIEFFAETDKETFAEIQRDLEGLKLPTFAEFV
                     AQERKKHRAKQAVKKARKIAKLARRKNR"
     gene            3491..3976
                     /locus_tag="PXD66_002144"
     CDS             3491..3976
                     /locus_tag="PXD66_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320396.1"
                     /translation="METALQLAQHLTGWTEEENSQAILKNFLDWKETFGEKTREETKI
                     VETLLDWINENEASFIEYPFNQEQRTPPKVAGYRILANNSTKTNEYFFIYPKQFKETL
                     AEFPLLMACTKLHEAGILHKPPRPEKGYEFQYTIAPKITGGSKKRAYKIVPIALEEEN
                     E"
BASE COUNT         1505 a          851 c          784 g          986 t
ORIGIN      
        1 taacggcgga gatgaaatag aaaaaatatc aaagaataac aaaactcaat tcagctcaac
       61 aatacgagaa aaccaaatca attttattag agccttgctt tatatggatt atgggataga
      121 aacgcctcaa gaagtaaaaa acgccattaa ttcaggggaa ctaggcagaa aaatagagaa
      181 atttaggaga gaaaacccca atatagagaa cgataaaaat ttcaaattcc ctacaagtgt
      241 aacccttagc aactggtatt caaaagccta aaatattgga aatttccgaa tctactttgg
      301 aaatttccaa acttctaaaa cctactcacc tcacaatctc ccccaacaat gcaactacaa
      361 aagacaataa gcacattcac gcaaccaaac cacaggggga tttatgtaat gacctattca
      421 gaacaaagct atttaaacgt gaaagatgta tgcgaaatgc tacaactcaa ccgctcaact
      481 atttatcgac aaattaagca agggattttc cctaagccaa ctaagatcgg aaaatcaagc
      541 cggtggaaat ttgccgacat caaagcattt ttagagccaa aaccacagga ggcgaactaa
      601 tggcagggct ttctattatt tgcccgtttt gtggcaatac cgcacgaatt agaggcagtt
      661 ctcgccctag ccttttaagt tatcaagcac agctaacttg ttcaaattgc ggacaattaa
      721 aagccgactt tgtagggcag ctaatcaaca tcaaacgggc cgtatttatt gagtgtgatg
      781 aagcaaatca atgggataaa cccgaaaatg aactaatcaa ggagggaaaa attcaaggca
      841 aagacaatgc tcaacgcctc aaagaactaa gaggctcaca gcaagattta tttgcaaagg
      901 tgccggggca actaccgccc gagaagatca ctccggcgga gaaaatcgcc aagcgggcaa
      961 acctttcaaa aacgcattaa acagggggat aaatggcaac aaaaaagccc cgcaaggcaa
     1021 accatacgga gctattaaaa tcgcataatg cgaaagatat gcaaagaaat taaagcaggc
     1081 ttgacgattt ctgaaggtgt tagattcttt ctaaagtctt tagggatagg tttatttcta
     1141 gcattgtttt tttatttttt gccgtattta gcagaattta taatcgccgt aaaaaaacaa
     1201 ctagcaaaaa aatcggctta ctttcaatga taaaagcaag ccgaaatgac aatccaaatt
     1261 tttaacttta aataaagagt acaagaagaa atgaaagatt tatctccaaa tcagaaacaa
     1321 tatacgccat tttcggctat aatgcaaaaa aatttaatgg atttattgac aaaatccgcc
     1381 cgttcgcctt attctttctc taccgttgca aaatcaacgg cagggcgtga gaaccctgaa
     1441 ctacataata cggcgaacag tagcacgcct ttaacccgtg ctatttttat tcgtagctta
     1501 cacacaccta aagaacgccg aaataaggcg tgttcgtcta tggtagcgtg tagcgggcaa
     1561 ggttcgccct tggctgtttt tggttcgccg tttgtagcag ttttctcacc ccgttacacg
     1621 ctatcgccct gcaccgtgag aagtgtagcc gatagtcctt taaaattaac aaacggacta
     1681 caaaaaatga accctagcca aattacaggc aaaaaccgcc taaactcttt tctatttaac
     1741 aataatcaaa atgattattc gctattattt actaatgcaa aacaatcaac cccgatttct
     1801 gctaaaataa gcaaaaatca agatttatgc ttgacaaccc tcaatcgttc gccttatgct
     1861 agccgtgctt ttgcaaaatc aaaagccgag tgtggaaact cgaaagaaca tcaggcgaac
     1921 aatagcacgc ctttaactcg tgcttttttt gttcgtagct cacgcacacc caaagaatgc
     1981 caaaataagg cgtgttcgtc tatggtagcg tgtagcgggc aaggtttcgc ccttgactgc
     2041 ttacctgatg ttgcagtttt ccaccccgtt acacgctatc gccctacatt gtggaaaatg
     2101 ttagcgatag cccctaaaaa tcaacatcag gagctatcaa aaatgaaagc attcgcttat
     2161 tcattccttt gtgctattcg cacacccgaa acagatactt atcaagaaac cgtagtacgc
     2221 attatttcag acagcgaaca aaacgcccgt tttcagctta cagccgatta tcgcttacta
     2281 ctcccccgcc ctgttgcaaa aatccgccta aattctaccg cttacacggt gaaagggggg
     2341 atttatgctt aattattccg atattgaaca aatccgctat tttatggatc aagccgaaga
     2401 ggctttagac aataatgaag agttcaaagc cgtagcagaa cttaacacag cacaacaatt
     2461 tattctggct ttattaatga actacgaaca agggggtgaa aatgaataat acaacaggac
     2521 aagctacgca aagaaactgg caagaaatta tcgctaattt gcagagactc agcgaatccg
     2581 gggaaagcac aatcgaaatc gctaaacgct atgaggcatt ttctaaaacc gccgagcaaa
     2641 tccaagccct agtgaataac aacggcagcg aagccgaaaa agaggcgttt tttgcccctg
     2701 ttgagcgttt ttatcttggc ttactagaaa ccgaaacacg cttaaaagat gccatagcca
     2761 aagacgaaga aaacgcccaa aaattgaaag agcaaaaagc agctagagag gcagagcaaa
     2821 aaagatggga caacctacct ttctacgcca aagacaaatt cagtgattta atttgcattg
     2881 aaagccgaga aacgcttgaa gataaattct ttgcgatctc ttgtatgttc gatgccctaa
     2941 aagccgtacc taatctatta gaaataggct tgcgtgaaga aggcaaagac agtgttatag
     3001 accgattagc cgaaatgagc aaactctatt tactgtttgt agatgagcaa ttttcactac
     3061 tggcaaacga aattcaaagc ggtaaatatg gcaagttaga cctaaacggc tcacagaata
     3121 agggggattt atgctaacgc accgcattac actccctgac ggcacagaaa ttgaattttt
     3181 tgcagaaacc gacaaagaaa catttgcaga aattcaaagg gacttagagg gcttgaaatt
     3241 gccaaccttt gccgagtttg ttgcccaaga gcggaaaaaa caccgagcaa aacaagcggt
     3301 caaaaaagcc cgaaaaattg caaaattagc aagacggaaa aaccgctagc acctatcaaa
     3361 aagacttatt attgcagaaa atcacgcttt aataaaaacg acctataaag caatccgcaa
     3421 gaaatggtta agtttaactg aaaatatgag cggacaggtt cagcgggtag cgtgcgatag
     3481 attcgcaata atggaaaccg ccttgcagtt agcccaacat ttaacaggct ggacagagga
     3541 agagaacagc caagcgattt taaaaaactt cctagactgg aaagaaactt tcggcgaaaa
     3601 aacaagagaa gaaactaaaa tagtagaaac cttgctagat tggataaatg aaaatgaggc
     3661 gagttttatc gagtacccat tcaaccaaga gcaaagaaca ccgcctaaag ttgccggcta
     3721 tagaatactt gcgaataaca gcactaaaac aaatgagtac tttttcattt accctaagca
     3781 atttaaagaa acgctggcag aatttccgct attgatggct tgcaccaagt tacacgaggc
     3841 agggatttta cataagccac cacgcccaga aaaaggctat gaatttcagt acacaatagc
     3901 ccctaaaata acaggaggga gcaaaaaaag agcttataaa atcgtaccga tagcattgga
     3961 agaagaaaac gagtaattac agcaaaagca agagtttcaa ttcttgcttt ttttatactt
     4021 caaacaaaat aaaaataaat acaaattaaa aatttatagg gaaaataggg aaaaaataag
     4081 aagaatatag atatatcaag gttttataga tttttatttt ccctat
//
LOCUS       DANLXF010000210         4106 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.210, whole genome shotgun sequence.
ACCESSION   DANLXF010000210 DANLXF010000000
VERSION     DANLXF010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.210"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            25..1026
                     /gene="pdxA"
                     /locus_tag="PXD66_002145"
     CDS             25..1026
                     /gene="pdxA"
                     /locus_tag="PXD66_002145"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HDL6320397.1"
                     /translation="MKPILGITMGDAAGIGPEVIIKALANQRIYELAHPVVIGDRKII
                     QRALPIVHSELKLRILNNLDNFVAEYGVIDILDLNNLPEDLPFAQVDPRAGKAAYEYI
                     EEAVNLAMGGKIHAIVTAPLNKEALHAGGKPFPGHTEILATLSNTKDYSMMLTSEKLN
                     VIHVTTHVQLRKACDLITKERVATVIDLAEENARILGFKEPRIAVAGLNPHSGENGMF
                     GDEDQKEIVPAIQEAQAKGFNVTGPVPPDTLFHRAANLNEFDIVVVMYHDQGHIPIKL
                     LGFDTGVNVTVGLPFIRTSVDHGTAFPIAGKGTADSRSMTEALYLGAKMANIKYATH"
     gene            1037..2035
                     /locus_tag="PXD66_002146"
     CDS             1037..2035
                     /locus_tag="PXD66_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HDL6320398.1"
                     /translation="MSTQTSKSIVPIYDGMNKIPGGAMVIPLILGSIIGTFFPEFLNL
                     GSFTTALFKSSAGPLIALLIFATGMQITVRSSGPVLAHTGVVLLFKTIIPALLVVILG
                     LWVGNDGVMGVSLLAMLTVVANSNGGIWLAFTGKYGDYRDKGAYVASAVNDGPFFVLL
                     FLGVSGLADIPLSLMLAAVIPLIIGMVIGNLDPKWTELMKPTGAIVIPFFAFALGTGI
                     NLQSIITGGATGIILGIVASLFTGFLVFIGYKTILRRGNQSGIGFAAGTTAGNAIITP
                     EIVAQADPTLAPFVQTATAQIAAAVLVSAILAPLFAAWVLKRQGGLEQDTIKGENL"
     gene            2032..3258
                     /locus_tag="PXD66_002147"
     CDS             2032..3258
                     /locus_tag="PXD66_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HDL6320399.1"
                     /translation="MKETLLVIADDLTGANDTAVMFAESGFDTVLKTKVSALAQIHPD
                     KAQVISVSTDSRAIGEKAKELTQIAISNAIQNSIGQIYLKIDSTMRGSVKYQIEGAIK
                     AWAGLYPDVKAIICPAYPEMGRTIEAGHLYVNGVPVNETASGKDPICPVHSSSMQKLL
                     PDAQVIPCDTVDNLQASIENSEYKQIVIDAKTHEDLSMIAQVINHIGNKIIPVGSAGL
                     AQKLKNIQNQQQPERNLKLGRALIVVTSIHETSQLQVDEYISTLGGKSIVFNPSPSQL
                     INYSQSEHSLKLQLNALIRFSRDHVIIRANPAKVVNAKSANITETSKEISQHLADLSK
                     FCLDNEKFDSLILFGGDGAAALLDQMNVTEMNVQYAVVPGVPLCSIEKGNYSGITVMT
                     KSGGFGNNTLLSNIMN"
     gene            3366..>4106
                     /locus_tag="PXD66_002148"
     CDS             3366..>4106
                     /locus_tag="PXD66_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL6320400.1"
                     /translation="MSKNRLEQILLFLKNHNIATVEQLVKVTEASPATIRRDLIKLDE
                     EGVVIRTHGGVSLNQFIPFQPTTNEKQFKQIVEKERIAEYAVSLISPGNSILLDAGTT
                     TLCIAKKIAHMPLRVITTDLHIALLLSEYKQIELIITGGRVDNSSQSCIGNHSLTLLE
                     NINPDIAFVSCNSWSLEKGVTAPTEEKANLKRMLQQNAKQKVLVADSSKYGKYSLFRA
                     TQLDNFTQIITDKGLPIEYSQALSSNDFK"
BASE COUNT         1343 a          728 c          769 g         1266 t
ORIGIN      
        1 attaatttca atcaaggagt aaatatgaaa cctatattgg gaattacgat gggagatgcc
       61 gccggtatcg gaccagaagt tattattaaa gcattagcta atcaacggat ttatgagtta
      121 gcacaccctg ttgtgatcgg tgatcgaaaa attatacaac gagccttacc tattgttcat
      181 tcagaattga aattaagaat tctcaataat ctagataact ttgtagccga atatggcgtt
      241 attgatattt tagatttgaa taacctgcct gaagatttgc cttttgctca agttgatccg
      301 cgtgctggaa aagcagctta tgaatatatt gaagaggcag ttaatttagc aatgggagga
      361 aaaatccacg ctattgttac agcaccactg aataaagaag ccctacacgc aggtggtaaa
      421 ccatttcctg gccatacaga gatccttgca accctcagta atactaaaga ttactcaatg
      481 atgctaacca gtgaaaaact caatgttatt cacgttacta ctcacgtcca acttcgtaaa
      541 gcctgtgatt taattaccaa agaacgagtt gctacagtta ttgatttagc agaagaaaat
      601 gctcgcattc ttgggtttaa agaacctcgt attgcagtcg ctggtttaaa tcctcattca
      661 ggtgaaaatg gaatgtttgg cgatgaagat caaaaagaaa ttgttcccgc aattcaagag
      721 gctcaagcca aagggtttaa tgttacaggg cctgtccctc cggatacttt atttcaccgt
      781 gcagctaacc taaatgaatt cgatattgtt gttgtgatgt atcacgatca agggcatatc
      841 cctatcaaat tattaggctt tgatacaggt gtaaacgtta ctgtcggtct tccatttatt
      901 cgaacatctg tcgatcacgg aactgctttc ccaatagcag gcaaaggaac agcagatagt
      961 cgtagtatga ctgaagcact ctatttgggt gcaaaaatgg caaatatcaa atatgcgaca
     1021 cactaaggag attaatatgt ctactcaaac atctaaatcc atcgtcccga tatatgatgg
     1081 tatgaataaa attcctggcg gtgcaatggt aataccgcta attcttggtt ctattattgg
     1141 tacattcttt cctgaatttt taaatttagg ttcatttacg acggctttat tcaaatcatc
     1201 agcaggacca cttattgcac tattaatttt cgctacaggt atgcaaatta ccgtgagaag
     1261 ttctggacct gtacttgccc ataccggtgt tgtattacta tttaaaacta ttattcccgc
     1321 tcttttagtt gtgattttag gattatgggt aggtaatgat ggtgttatgg gcgtttcttt
     1381 attagctatg ctaactgttg ttgcaaattc aaatggtggt atttggttag cgtttacagg
     1441 gaaatatggt gattatagag ataaaggagc ttatgtcgct tcggctgtta atgatggacc
     1501 tttctttgtt ttactatttt taggtgtatc aggattagca gatattcctc tttcattaat
     1561 gcttgcagct gttattcctc ttattattgg tatggttatt ggtaacttag atcctaaatg
     1621 gacagagtta atgaaaccaa caggtgcaat agtcattcct ttctttgctt ttgcattagg
     1681 aacaggaatc aatctgcaat ctattattac tggaggagca acaggaatta ttttaggtat
     1741 cgtagcttct ctctttacag gttttcttgt atttatcggt tacaaaacaa ttttaagacg
     1801 tggtaatcaa tcaggaatcg ggtttgcagc aggaactact gctggaaatg ccattattac
     1861 acctgaaatt gttgctcaag cagaccctac attagcacct tttgttcaaa ctgcgactgc
     1921 acagattgcc gcagctgtat tagtttcagc aatcttagct ccattatttg cagcgtgggt
     1981 tttaaaacgt caaggtggct tagaacaaga cactataaaa ggagaaaatc tatgaaagag
     2041 acactcttag ttattgctga tgaccttact ggtgcaaatg ataccgcggt tatgtttgct
     2101 gaatcaggat ttgacactgt cttaaaaact aaagtttcgg cattagccca aattcatcct
     2161 gataaagccc aagtaatttc tgtttctaca gattcaagag caattggaga gaaagcaaaa
     2221 gagctgactc aaattgcaat tagcaatgct attcaaaata gtatcgggca aatttacctt
     2281 aaaattgatt ccacaatgag aggctctgtt aaatatcaga ttgaaggtgc tattaaagct
     2341 tgggctggtt tatatcccga tgttaaagca attatttgtc ctgcatatcc agaaatggga
     2401 agaactatag aagctggtca tttatatgtt aatggtgttc ctgtaaatga aactgcttca
     2461 ggaaaagatc ctatctgccc tgtgcattca tcttctatgc aaaaactatt gcctgatgct
     2521 caagtaatcc cgtgtgatac ggtcgataat ttgcaggcat ctattgaaaa tagtgaatat
     2581 aaacagatcg ttattgatgc taaaacgcac gaagatctct caatgattgc ccaagtcata
     2641 aatcacatag gtaataaaat tattcctgtt ggctcagccg gattggcaca gaagttgaaa
     2701 aatatccaaa atcaacaaca accagagcga aatcttaaat taggtagagc attaattgtc
     2761 gtaacctcaa ttcacgaaac aagccaactg caagttgatg aatatatttc aactttagga
     2821 ggaaaatcta ttgtatttaa tccttcgcct tcacagttga ttaactactc gcaatcagaa
     2881 cattcactga aactacaatt aaacgctcta atacgtttta gtagagatca tgttattatt
     2941 cgggcaaacc ctgcaaaagt tgtaaatgca aaatctgcca atatcacgga aacttctaaa
     3001 gaaatttctc aacatttagc tgatttaagt aaattctgcc ttgataatga aaaatttgat
     3061 agccttatat tatttggtgg agatggtgca gcagcattat tagatcaaat gaatgtaaca
     3121 gagatgaatg tacaatatgc ggtagtaccg ggcgttcctt tatgctccat tgaaaagggc
     3181 aactactcag gcatcactgt aatgactaaa tctggaggat ttggaaacaa cacactctta
     3241 agcaatataa tgaactagta acaacaaaga acctaaagca acaatgtttt aggttcttta
     3301 attttaacta tcttattttt aaaaaattac ttatagtaaa agcagttaaa aattagagag
     3361 tttatatgtc taaaaaccgt ctagaacaaa ttttactatt cctaaaaaac cacaatattg
     3421 ctaccgtaga gcaactggta aaagtgactg aggcttctcc tgcaactatc cgccgtgatt
     3481 taataaaatt agatgaagaa ggcgtcgtga ttcgcacaca cggaggggtc agcttaaacc
     3541 aatttatacc gtttcaacca acaacaaatg agaagcaatt taagcaaatt gtcgaaaaag
     3601 aacgcatagc tgaatatgcc gtttcgctta tttcaccggg aaattctatt ttattagatg
     3661 ctggcactac aacgctatgc attgcgaaaa aaatcgctca tatgccactt cgagtgatta
     3721 caactgatct acatattgct ttattactct ctgaatataa gcaaatagaa ttaattatta
     3781 cagggggacg agtagataat agtagtcaat cttgtattgg taatcatagt ttaactttat
     3841 tagaaaatat taatcctgat attgcttttg tgagctgtaa ttcttggagc ttagaaaaag
     3901 gagttacagc acctacagaa gagaaagcta atttaaaaag aatgctccaa caaaacgcca
     3961 aacaaaaagt actggttgcc gatagcagca aatatggaaa atactcttta tttcgtgcaa
     4021 ctcaactcga taactttaca caaattatca cagataaagg actacctatt gaatatagcc
     4081 aagccttgtc aagtaatgat tttaag
//
LOCUS       DANLXF010000211         4074 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.211, whole genome shotgun sequence.
ACCESSION   DANLXF010000211 DANLXF010000000
VERSION     DANLXF010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.211"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            71..712
                     /gene="sodA"
                     /locus_tag="PXD66_002149"
     CDS             71..712
                     /gene="sodA"
                     /locus_tag="PXD66_002149"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005708524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HDL6320401.1"
                     /translation="MAYTLPELGYAYDALEPHFDAKTMEIHHSKHHQAYINNANAALE
                     AHPELLEKCPGALIKDLSQVPAEKRIAVRNNVGGHVNHTLFWKGLKTGTTLQGALKEA
                     IERDFGSVEAFQSEFEKAATTRVGSGWAWLVLEEGKLAVVSTANQDNPLMGKEVAGVS
                     GYPILVLDVWEHAYYLNYQNRRPDFIKAFWNVVNWDEAARRFEEKVATCGCAK"
     gene            complement(775..1899)
                     /gene="alr"
                     /locus_tag="PXD66_002150"
     CDS             complement(775..1899)
                     /gene="alr"
                     /locus_tag="PXD66_002150"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDL6320402.1"
                     /translation="MKPATATIDSQALRHNIRLIKSFAPQQKLLAMIKANAYGQGLLP
                     AAGTLADMVDGFGVARLREALEIQETGYTGKIVLVEGFFDREELLKTLSRKFDTVIHN
                     LEQLELLEQVAKEWQAEQQKGFWTRKAKIYFPINIWLKIDTGMHRLGIHPEQVDHFVE
                     RLKNCALVNSISFVSHFSRADELDCGYTEKQIAVFEQATQKYQDHDRSISASSGILYW
                     KQAHYDWVRPGIIMHGISPHYTPITDLGFKPVMTLSSSLIAVRTHKAGEPVGYGGAWV
                     SPKDTKLGVTAMGYGDGYPRNAPEGTPVLINGRIVPIVGRVSMDMLTVDLGAESQDKV
                     GDKAIFWGEQLLIEDVAKHIGVISYELITKLTPRVIFEYK"
     gene            2029..3990
                     /gene="recD"
                     /locus_tag="PXD66_002151"
     CDS             2029..3990
                     /gene="recD"
                     /locus_tag="PXD66_002151"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HDL6320403.1"
                     /translation="MLNLLLELKQEKAISDLNYQFARFIDNKQKPYGYSPLEQDLAVL
                     AAALVSYQVGLGHTALRLENLSLSSLFELNSKPQTYHLLEQILQKIEQIPPLEWQSVL
                     KNHIAFSYSPETIKPMLFQNSLLYFYRYWQAEHRIAGYLQQAVDFSEKFANTELNQSI
                     LNQLFTGKAQTIDWQKIAVATALEKQFSLISGGPGTGKTTTVVKLLLGLQLKQQALQK
                     PYLQIALAAPTGKAAARMKESLESKLAEEKGLSEELKRSIPTEAVTIHRLLGARPLTE
                     KTKYHTKNPWHYDLVVLDEASMIDLSMMEKVVQALKPNSRLIMLGDKDQLASVEAGSI
                     MGELGSFIDNGYSQSHCDYLKAVTDYQIEAGNALPIGDCLSHLKHSYRFGEKAWIGEL
                     ANAVNDQKINLSWEIFDKYKNSDKLENFCYPETKETTDKSSWIEKSVQMVVDKAVELY
                     RDYLQAVQKREKNPDSVSVKAIFEKFQKVRILSALRVSELGVERLNMAIAEGLRKASL
                     VKFNHSRDRYIGKPILMTENMPINSIASGDVGIILPDENGESRVYFDSLQENREHHSL
                     PLGRVSNYEAAYIMTVHKSQGSEFEHTVLVLPHSISPVLTKELIYTAITRAKDKFTLF
                     GSEKVWKYSVGAKTERQSGLKEQIRGKFS"
BASE COUNT         1268 a          787 c          877 g         1142 t
ORIGIN      
        1 gtgtttcttg ataaaatgta ctcggagttt aactctgatt aacctattta ctatttgaga
       61 ggaattaact atggcatata cattaccaga attaggctac gcttatgatg cgttagagcc
      121 acatttcgat gctaaaacaa tggaaatcca ccattctaaa catcaccaag cttacatcaa
      181 caatgcaaat gcagcattag aagctcaccc tgaattatta gaaaaatgtc cgggtgcatt
      241 aatcaaagat ttgagccaag tgcctgcaga aaaacgtatt gcagtgcgta acaacgtagg
      301 tggtcatgta aaccacactt tattctggaa aggtttaaaa acaggcacta ccttacaagg
      361 tgcattaaaa gaagcgattg agcgtgattt cggttcagtt gaagctttcc aatcagaatt
      421 tgaaaaagca gcgactactc gtgttggttc aggctgggcg tggttagtat tagaagaggg
      481 taaattagcc gttgtttcaa ccgctaacca ggacaaccca ctaatgggta aagaagtggc
      541 aggcgtttca ggctatccga ttttagtctt agacgtttgg gaacacgctt actacttaaa
      601 ctaccaaaac cgccgtccgg atttcattaa agcattctgg aacgttgtaa actgggacga
      661 agccgctcgc cgttttgaag aaaaagtagc aacctgcggt tgtgcgaaat aatattttcg
      721 atcttgaata agatacaagc ggtaaaattt gttgaaaatt ttaccgcttg ttgtctattt
      781 atattcaaaa atcacacgag gagtgagttt agtaatcagc tcatagctaa tcacgccaat
      841 gtgtttcgcc acatcttcaa ttaatagttg ctctccccaa aaaatggctt tatcgcccac
      901 tttatcttgg ctctctgcac ctaaatcgac cgtcagcata tccatcgaaa ctcgaccgac
      961 aatcggcact attctaccgt taatcaacac aggggtacct tcaggagcat tgcgagggta
     1021 gccatcgccg tagcccattg cagtcacgcc gagtttggta tctttagggc ttacccaagc
     1081 accaccatag cctacaggct cgccggcttt atgggtacgc acagcgatca gggaagagga
     1141 gagcgtcatt accggcttaa aacctaaatc ggtaatcggc gtatagtggg gagaaatgcc
     1201 gtgcataata atacccgggc gaacccaatc ataatgagcc tgtttccaat aaagaatacc
     1261 gcttgaggcg gaaatactgc ggtcgtgatc ctgatatttt tgcgtagctt gctcaaacac
     1321 cgcaatttgt ttttcggtat agccacaatc gagttcatcg gcacggctaa aatgactcac
     1381 aaagctgatt gaattgacca aggcacaatt tttcaaacgt tcaacaaagt gatcgacttg
     1441 ctccgggtga atgcctaagc ggtgcattcc ggtgtcgatt tttagccaaa tgttaatcgg
     1501 gaagtagatt ttcgctttac gagtccaaaa gcctttttgt tgctctgcct gccattcttt
     1561 tgctacttgc tcgagtaatt ccagttgttc gaggttatga atgacggtat caaacttgcg
     1621 ggaaagggtt ttgagtagct cttctcggtc aaaaaaacct tcaactaaca caattttgcc
     1681 tgtgtatccg gtttcttgaa tttctaaggc ttcacgcaaa cgggcaactc caaagccatc
     1741 aaccatatct gctaatgtgc cggcagccgg caataagcct tgcccgtaag cattggcttt
     1801 gatcatcgcc aataattttt gctgtggagc gaaagatttg atcaggcgaa tattatggcg
     1861 gagggcttga ctatcaatgg ttgctgttgc tggtttcata cttatctaca tagttaaaat
     1921 cacaagaaag gctaaattat acgcctaaaa taaaaaatcc cttagaaaat aatgcaaaaa
     1981 agattagtat tatcacttaa tgttaggtgg tcgatttaag gtttgattat gctgaattta
     2041 ttattggaac ttaaacaaga aaaggcgatt tctgacttaa attatcaatt tgcccgtttt
     2101 attgataata agcagaaacc ctatgggtat tcgcctttgg agcaagattt agctgtgtta
     2161 gccgctgcgt tggtttctta ccaagtggga cttgggcata cggcattacg tttggaaaat
     2221 ctcagtcttt cttctctttt tgaattaaat agcaagccac aaacttatca tttgctcgaa
     2281 caaattttgc aaaaaattga gcaaattcca ccgcttgaat ggcagagtgt gctgaaaaat
     2341 catatcgctt ttagctattc tcccgaaaca attaaaccga tgttattcca aaacagcttg
     2401 ctctattttt atcgctactg gcaagcggag catcgcattg ccggttattt acaacaagcg
     2461 gttgattttt cggaaaaatt tgcaaatacc gaattaaacc aaagcattct caaccaacta
     2521 ttcactggga aagctcaaac tatcgactgg caaaagattg ccgttgcaac agcattagaa
     2581 aagcagttta gcttaatttc aggtgggccg ggaaccggaa aaacaactac ggtagtaaaa
     2641 ttattactcg ggctgcaatt aaaacagcaa gcactacaga aaccatattt gcaaatcgct
     2701 ctggctgcac caaccggaaa agcagcagca agaatgaaag agtcgctgga aagcaagttg
     2761 gcggaagaaa aggggttatc tgaggagtta aaacgctcta ttccaactga agcagtgact
     2821 attcatcgct tgcttggtgc gagaccatta actgagaaaa ccaaatatca taccaaaaat
     2881 ccgtggcact atgatttggt cgtattagat gaggcttcaa tgattgattt atcaatgatg
     2941 gagaaagttg ttcaagcgct caaaccaaac tctcgcttga taatgctggg agataaagat
     3001 cagcttgctt ctgtggaagc tggttcaatt atgggggaac ttggctcttt tattgataat
     3061 ggttatagcc aatcccattg cgattactta aaagccgtta ccgactacca aattgaagcc
     3121 ggtaatgcgt tgccgattgg tgattgttta tctcacttaa aacatagcta tcgatttggc
     3181 gaaaaagcct ggattggaga gcttgcaaat gcagtaaatg atcaaaaaat taacctttcg
     3241 tgggagattt tcgacaaata taaaaacagt gataagttag aaaatttctg ttatccggaa
     3301 acgaaagaga caacggataa atcaagctgg attgaaaaaa gtgtgcaaat ggtggtggat
     3361 aaagcggttg aactttatcg tgattattta caagcggttc aaaaacggga gaaaaatcca
     3421 gattcagtga gtgtgaaagc gatttttgag aaatttcaaa aagtccgaat tttatcagcc
     3481 ttaagggtaa gtgaactggg ggttgaacgt cttaatatgg cgattgcgga aggcttgcgg
     3541 aaagctagct tagtgaaatt taatcatagc cgagaccgct atatcggaaa accgatttta
     3601 atgacagaaa atatgccgat aaatagcata gccagcggtg atgtcggcat cattttgccg
     3661 gacgaaaatg gagaaagccg agtttatttt gattccttac aggaaaatag agaacaccat
     3721 agtttgcctc taggtagggt ttcaaattat gaagcagctt atattatgac tgtgcataaa
     3781 tcgcaaggtt ctgaatttga acatacggta ttggtgttgc cccattcgat ttccccggtg
     3841 ctgacgaaag agttgattta caccgcaatc acaagggcaa aagataaatt taccttattt
     3901 ggtagcgaaa aagtttggaa atacagtgtt ggtgccaaaa ccgaaagaca aagtgggcta
     3961 aaagaacaaa taagaggcaa gtttagctaa attaatactc gttttgccgt aaaaatacgc
     4021 aagcggtcat ttttttcaaa ttttttacaa aaaccgcttg cgttgttttt gttt
//
LOCUS       DANLXF010000212         4061 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.212, whole genome shotgun sequence.
ACCESSION   DANLXF010000212 DANLXF010000000
VERSION     DANLXF010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.212"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(100..1458)
                     /locus_tag="PXD66_002152"
     CDS             complement(100..1458)
                     /locus_tag="PXD66_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HDL6320404.1"
                     /translation="MANQQQERQSWSNRLAYIMTVAGATVGFGATWRFPYLVGEHGGG
                     AYVFLFCIAMIVIGIPMILVENVIGRRMRVNAIDAFAGFANGKKIASGWKILGYMGLL
                     GAFGILAYYMVLGGWVLNYIGSLLTGTLDLSQPVTVGTTQQFFQQSIFNSPMTIIAYT
                     ALFVLVNYFILVKGIVDGIERSVKYLMPILFIFLIVMVVRNITLPGAAEGIAFYLMPD
                     FSKITPKLFVFVLGQVFFALSLGFGVLITLSSYLDKSENLVKTATITGVMNTLIAVLA
                     GFMIFPSLFSFGVAPNSGPTLVFQSLPIVFSNMWGGTVFAVIFFSLLVVAALTTSLTI
                     YEVIITALQEKAKISRQKAIFITLATIFLLGNIPSALSDNILQDVQVMGKSIFDAFDY
                     ISGNILFILTALGSAIFVGFVLKEEAKEELGAGKMFTTIWFNYVRFVIPVIILVIFFN
                     SL"
     gene            complement(1528..2355)
                     /locus_tag="PXD66_002153"
     CDS             complement(1528..2355)
                     /locus_tag="PXD66_002153"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HDL6320405.1"
                     /translation="MNHPKHRAFLKWAGGKYRLIPDIQTHLPKKACLVEPFVGAGSVF
                     LNTDFERYILADINPDLINLFNVVKADVEEYIAQTKQLFLHPQANTEAFYKARRAEFN
                     RSKDIFRRSVIFLYLNRFGYNGLCRYNQKKGYNVPFGRYKTHYFPEKELRFFAEKAQK
                     ATFVCADFEQVFELAKNQLTDYVIYCDPPYAPLAQETNFTQYAGGGFSLEQQGRLATL
                     ALEMKGLGVPVLISNHDTDFTRRIYQSADITQIQVQRSIGQKVGSRVKVGELFALFI"
     gene            complement(2369..3457)
                     /gene="aroB"
                     /locus_tag="PXD66_002154"
     CDS             complement(2369..3457)
                     /gene="aroB"
                     /locus_tag="PXD66_002154"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDL6320406.1"
                     /translation="MLQVNVELKERRYPIIIGAGLLNQPASYSPLKSGDKVMIVSNPT
                     VATHYLSVVTNALKELGCHVDSVLIPDGEEYKTLESLNLIFTALLEKNHNRDTTLIAL
                     GGGVIGDVAGYAAASYQRGIRFIQIPTTLLAQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     VSVIVDTNTLHTLSKREVSAGLAEVIKYGAIFDVTFFEWLEKHIDDLVSLKQDELEYC
                     IQRCCQLKADVVARDETEKGDRALLNLGHTFGHAIEARMGYGVWLHGEAVSVGMLEAA
                     ELSRILGDLSPQEVARLEKLLARANLPTISPDGMEPAEYLPYMWRDKKVLAGQLRLIL
                     LKSLGEAYVSSQATESQVLASIERFTQR"
     gene            complement(3481..4002)
                     /gene="aroK"
                     /locus_tag="PXD66_002155"
     CDS             complement(3481..4002)
                     /gene="aroK"
                     /locus_tag="PXD66_002155"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005710312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HDL6320407.1"
                     /translation="MAEKRNIFLIGPMGAGKSTIGRQLAQTLGMEFMDSDSVIEERAG
                     ADIDWIFDVEGEEGFRKREERILNELTQNYGIVLSTGGGSILSKDNRNVLSARGIVIY
                     LETTVDKQFERTQRDKKRPLLQTEDPRKTLEELAKIRNPLYEEIADITIHTDEQSAKV
                     VANQIIDMIDNLQ"
BASE COUNT         1330 a          915 c          677 g         1139 t
ORIGIN      
        1 ttggttaatt aagcaagaat tgcaaattcc ccacaaaatt taaccgcttg tcactaaaaa
       61 taaagctgtt tgaaaatcca ttcaaacagc ttatagaatt tagagtgaat taaagaaaat
      121 caccaagata atcaccggaa tcacaaagcg aacatagtta aaccaaatag tggtaaacat
      181 tttacccgca cctaattcct ccttcgcctc ttcttttaga acaaagccaa cgaaaatcgc
      241 gctacctaag gcggttaaaa taaacagaat attgccacta atataatcga aggcatcaaa
      301 aatgctcttg cccataacct gcacatcttg caatatattg tcacttaatg ctgatggaat
      361 attacccaac agaaaaatag tagccaaagt aataaaaata gctttttgac ggctaatctt
      421 tgccttctct tgcaatgcag tgataataac ttcataaata gttaacgagg ttgttaatgc
      481 cgccaccact aatagactaa aaaagataac cgcaaaaact gttccccccc acatatttga
      541 gaacacaatc ggcaaacttt ggaatactaa agtcggacct gaatttggag caacgccaaa
      601 gctaaataat gacggaaaaa tcataaaacc ggcaagtacc gcaattaagg tgttcatcac
      661 cccagtaatt gttgctgttt ttactaaatt ctcgctctta tcaaggtagc ttgaaagggt
      721 tatcaacaca ccaaaaccaa ggcttaatgc aaaaaatact tgacccagca caaacacaaa
      781 caattttggt gtgatttttg aaaaatcggg catcagataa aaggcaatgc cttcagcagc
      841 ccccggtaag gtaatatttc gaaccaccat cacaatcagg aaaataaaca gaatcggcat
      901 caaatatttc actgaacgct caataccgtc cacaatgcct tttaccaaaa taaaataatt
      961 caccaataca aaaagtgccg tataagcaat aattgtcatt gggctattaa aaatactttg
     1021 ttggaagaat tgttgggtcg tgccaacggt aacaggttga gacagatcta gtgtccccgt
     1081 gagtaagcta ccgatataat ttaataccca accaccaagt accatatagt aggcaagaat
     1141 accaaatgct ccaagcaagc ccatatagcc aagaattttc caacctgagg caattttttt
     1201 accattagca aaaccggcaa aagcatcaat agcattcact ctcattcggc gaccaattac
     1261 attctccact aatatcatag ggataccaat tacgatcatc gcaatacaaa ataaaaacac
     1321 ataagcaccg cctccgtgtt cccccacaag ataagggaaa cgccaagttg caccaaaacc
     1381 gactgttgca ccagcaacgg tcataatata cgccaatcgg ttagaccaag attggcgttc
     1441 ttgttgttga tttgccattt aatactccat cggctaacaa taccgcttca accaacaagc
     1501 ggtcagattt ttagaaaata ttacaaatta aatgaagagt gcgaataact cgccaacttt
     1561 aacccttgag cccaccttct gccctattga acgctgtacc tgaatttggg taatatcggc
     1621 agattgataa atcctacggg taaaatcagt atcgtgattt gaaattaata cgggaacgcc
     1681 taatcctttc atttctaaag ccagtgttgc caaacgccct tgttgctcta ggctaaaacc
     1741 accgccagca tattgcgtaa aattcgtttc ttgtgctaaa ggagcgtaag gtgggtcgca
     1801 gtaaatcaca taatccgtta attgattttt tgccaactca aagacctgtt caaaatccgc
     1861 acacacaaaa gttgcctttt gtgctttttc agcaaaaaaa cgtagttctt tttctggaaa
     1921 ataatgggtt ttataacgcc caaagggaac attgtaccct tttttctggt tataacggca
     1981 aagtccgtta tagccaaatc gatttaaata caaaaaaatc accgagcgac gaaaaatatc
     2041 ttttgagcga ttaaactccg cacggcgtgc tttataaaag gcttctgtat tcgcttgtgg
     2101 gtgaagaaac agttgctttg tttgcgcaat atattcctca acatctgctt tcaccacgtt
     2161 gaaaagatta atcaagtcag gattaatatc agcaagaata taacgttcaa aatctgtatt
     2221 cagaaagacc gaacctgcac caacaaaagg ctcgactaaa caagcctttt ttggcaaatg
     2281 ggtttgaata tctggaatta agcgatattt accgccagcc cattttaaaa aggctcgatg
     2341 cttagggtga ttcatttcat tttaaacatt aacgttgggt aaaacgctca atagaggcta
     2401 atacttgaga ttctgtggct tgcgatgaaa cgtaagcttc accaagtgat ttcaataaaa
     2461 ttaggcgtaa ttgacctgcc agtacttttt tatctcgcca catataaggt agatattctg
     2521 ccggttccat tccatcaggt gaaattgttg gcaaattggc tcgggccaat aatttttcca
     2581 agcgagcaac ctcttgagga cttaaatccc ccagaatacg agataattcc gcagcctcta
     2641 gcataccaac cgaaacagcc tctccgtgta accatacacc atagcccata cgtgcttcaa
     2701 tggcgtgacc aaaggtatgt cctaagttta gtaatgcacg atctcctttt tccgtttcat
     2761 ctcgagcaac cacgtcagct tttagctggc aacaacgctg aatacaatat tccagctcat
     2821 cttgcttgag actgaccaaa tcatcaatat gcttttcaag ccattcaaaa aaagtaacat
     2881 caaagattgc accgtatttg atgacttccg ccaaaccagc actgacttct cttttggata
     2941 aggtatggag cgtattggta tcaacaatca cagaaacagg ctgataaaaa gccccaatca
     3001 tatttttacc gagtgggtgg tttactgccg ttttcccgcc tactgaagaa tcaacttgag
     3061 ccagtaaagt ggtcggaatt tgtataaaac gaataccacg ttggtaagag gcagctgcat
     3121 agcctgcaac atcaccgatt acaccaccgc ctaaagcaat taaagtggta tcgcgattat
     3181 gatttttttc taataatgcg gtaaaaatca aatttaatga ttctagcgtt ttatattctt
     3241 caccgtcggg aatcaatacc gaatcaacgt ggcaacccag ctcctttaac gcatttgtta
     3301 ccacactaag ataatgtgtc gccaccgttg gatttgagac aatcatcacc ttatctccag
     3361 attttaatgg gctataacta gcaggctgat ttaataaccc tgcaccaatt ataatcgggt
     3421 aacgacgctc ttttaattcc acattgactt gtaacatatc ttagctcctt tcttacctgg
     3481 ctactgtaaa ttatcaatca tatcaataat ttgatttgcc actactttag cgctctgctc
     3541 gtccgtatga atagtaatat ctgcaatttc ttcatacaat gggttacgga ttttcgctaa
     3601 ctcttccaac gttttacggg ggtcttctgt ttgcaataaa gggcgttttt tatctcgctg
     3661 agtacgttca aactgtttat ccactgtcgt ttctaaataa ataacgatac cacgagccga
     3721 taacacatta cggttatctt tagataaaat cgaaccaccg ccagttgaaa gcacgatacc
     3781 ataattttgc gtcagttcat ttagaatacg ttcttcacgc ttacggaaac cctcttcacc
     3841 ttcaacatcg aagatccaat caatatccgc acctgctcgt tcttcgatca cactgtcgga
     3901 atccataaat tccataccta atgtttgagc aagctgacga ccaatcgtac ttttacctgc
     3961 tcccatcggc ccaatcaaaa aaatattacg tttttcagcc attatttctt cttcatttta
     4021 aaatttacta aacatttgat ctgtgaaatt aaaagaaaaa a
//
LOCUS       DANLXF010000213         3910 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.213, whole genome shotgun sequence.
ACCESSION   DANLXF010000213 DANLXF010000000
VERSION     DANLXF010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.213"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(13..1653)
                     /gene="pgi"
                     /locus_tag="PXD66_002156"
     CDS             complement(13..1653)
                     /gene="pgi"
                     /locus_tag="PXD66_002156"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013527751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDL6320408.1"
                     /translation="MQKINPVQTFAWNALEQHKADNLSIPQLFAEDPNRFDKYSLRFE
                     DQLLVDFSKNAINEKTLTLLRQLANECGLESAKNAMFSGKKINRTENRAVLHVALRNR
                     ANTPIAVDGKDVMPEVNAVLAKMKDFCERVISGEWKGYTGKTITDVINIGIGGSDLGP
                     YMVTEALRPYKNHLNMHFVSNVDGTHIAEVLRKVNPETTLVLVASKTFTTQETMTNAL
                     SARKWLLDSAKDESAVAKHFVALSTNAKEVEKFGIDTANMFEFWDWVGGRYSLWSAIG
                     LSIALSLGFENFEQLLAGAHAMDKHFLITPIEKNIPTTLALVGIWNNNFLGAESEAIL
                     PYDQYMHRFAAYFQQGNMESNGKFVGRDGKPVTHQTGPIIWGEPGTNGQHAFYQLIHQ
                     GTKLIPCDFIAPAQTHNPLSDHHNKLLSNFFAQTEALAFGKSKETVEKEFLDAGKTLE
                     EVAEIVPFKVFSGNKPTNSILVQKITPYSLGALIAMYEHKIFVQGVLFNIYSFDQWGV
                     ELGKQLANRILPELETDEKINSHDSSTNGLINQFKAWR"
     gene            complement(1686..1934)
                     /locus_tag="PXD66_002157"
     CDS             complement(1686..1934)
                     /locus_tag="PXD66_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HDL6320409.1"
                     /translation="MLTPKNLEAIAQQLHNALPQSLKNVGNDLDEKFKQILQAQLARL
                     DVVTREEFDVQSQVLLRTREKLTELEKRLDELTQRTDE"
     regulatory      2058..2102
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            2106..2774
                     /gene="queC"
                     /locus_tag="PXD66_002158"
     CDS             2106..2774
                     /gene="queC"
                     /locus_tag="PXD66_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HDL6320410.1"
                     /translation="MTHQKPKAVVIFSGGQDSTTCLFQAIAEFGKENVEVVTFQYGQR
                     HSIELEKARWISNDLGVKQTLIDTSVIKAITTNALMDNNAEIQQQGSTPNTFVDGRNA
                     LFLLYTAIYAKGQGIQTIFTGVCETDFSGYPDCRDVFVKSMNVTLNLAMDYNFNIRTP
                     LMYLTKKETWALADKLGAFDYIRDHTHTCYLGVEGGCHDCPSCVLREKGLNEYLAERE
                     VSNV"
     gene            2767..3189
                     /gene="queD"
                     /locus_tag="PXD66_002159"
     CDS             2767..3189
                     /gene="queD"
                     /locus_tag="PXD66_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HDL6320411.1"
                     /translation="MFKIAKEFSFDMAHMLDGHDGKCQNLHGHTYKLQVEVAGELFQE
                     GAKRGMVMDYSDLKAVVKQHILEPMDHAFIYDLQSERESQVAQLLIDLNSKVYGIPTR
                     TTAEEMAKYMFEKLEKVGLPVSLIRLWETPTSYCEYSK"
     gene            3189..3815
                     /locus_tag="PXD66_002160"
     CDS             3189..3815
                     /locus_tag="PXD66_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HDL6320412.1"
                     /translation="MAELISNPRFPIVEIFESLQGEGFNTGMPAIFVRFGKCNLTCPW
                     CDTNYNQFEQWTLSDILAKVRSYSAKNVIITGGEPTIQPNLSLLLEQFKKEGYFLAIE
                     TNGLKEVPAQIDYIATSPKRMYQEKYQRRCIEFANEVRIVVDGEVQGFCEQIESQIKA
                     EHYYLSPCEVEGKMNLLETITQIGLLNQRPNKPKWQLSIQTHKIVGIE"
BASE COUNT         1168 a          734 c          835 g         1173 t
ORIGIN      
        1 tattgactat atttaacgcc aagccttgaa ttggttaatt aaaccatttg tggagctgtc
       61 gtggctgttg attttttcat cggtttcgag ttcgggtaaa atgcggtttg caagctgttt
      121 gcctaactcc acgccccatt ggtcgaaact gtaaatgttg aacagcacgc cttgcacgaa
      181 gattttgtgt tcatacattg caattaatgc ccctaagctg tacggggtga ttttttgcac
      241 caagatagag ttggtcggtt tgtttccgct gaacacttta aacggcacga tttcagctac
      301 ttcttccaac gttttacctg catctaaaaa ctctttttca actgtttctt ttgatttacc
      361 gaaagctagt gcttcagttt gagcaaagaa gttagaaagc agtttgttgt gatgatcgct
      421 taacggattg tgagtttgtg ccggtgcgat aaaatcacaa ggaattaatt ttgtgccttg
      481 atgaatgagt tgatagaatg cgtgctgacc gttagttccc ggttcgcccc aaataatcgg
      541 acctgtttgg tgagtaaccg gtttgccgtc acgccccaca aatttaccgt ttgattccat
      601 attgccttgt tggaaataag ccgcaaagcg gtgcatatat tggtcgtaag gtaaaatcgc
      661 ttctgattca gcacctaaga aattgttatt ccaaatgccg actaaagcta acgtagttgg
      721 aatgtttttc tcaatcggcg tgattaagaa atgcttatcc attgcgtgag caccagcaag
      781 tagctgctcg aagttctcaa aacctaatga aagggcaatc gaaagcccga tggcagacca
      841 taatgagtaa cgaccgccaa cccaatccca gaattcaaac atattagcgg tatcaatacc
      901 gaatttttcc acttctttgg cattggttga aagtgccacg aaatgttttg ctaccgcaga
      961 ttcatctttg gcagaatcaa gtagccattt gcgagctgaa agggcattgg tcatcgtttc
     1021 ttgagtggta aaggtttttg aagccactaa cactaacgtg gtttcaggat taactttacg
     1081 caacacttca gcaatatgcg tgccgtccac gttagacaca aagtgcatat taaggtgatt
     1141 tttataaggg cgtaatgcct ctgttaccat ataaggacct agatccgagc caccaatacc
     1201 gatattgatc acatcagtaa tcgttttgcc ggtatagcct ttccattcgc cggaaataac
     1261 acgctcgcag aaatctttca ttttcgctaa cacggcattc acttccggca ttacatcttt
     1321 gccatcgact gcgattggtg tattggcacg gttacgcagt gcaacgtgca atacggcacg
     1381 gttttcagtg cggttgattt ttttgccgga gaacatcgca ttttttgctg attccaaacc
     1441 acattcattt gccagttggc gaagtaaggt taaggttttt tcgttgatcg cattttttga
     1501 aaaatcgact aataattgat cttcaaaacg gagggaatat ttgtcgaaac ggtttggatc
     1561 ttcggcaaaa agttgaggaa tagaaaggtt gtctgcttta tgttgctcta aggcattcca
     1621 agcaaaagtt tgcacagggt taattttttg catctgagtt tccttatact aaattaaaaa
     1681 atcgattatt catctgttct ttgagtcagt tcatcaagac gtttttctaa ttcggttaat
     1741 ttttcacgag tgcggagtaa cacttgagat tgaacatcaa attcttcacg agtgacaaca
     1801 tcaagacggg ctaattgggc ttgaaggatt tgcttaaatt tttcatctag atcattaccg
     1861 acatttttta agctttgcgg taaggcgtta tggagttgtt gagcaatcgc ttctaagttc
     1921 ttcggcgtga gcatatttaa attccttact ataaatgagt atcccaattt tattgaattt
     1981 tgggtttaag agaaagcaaa ttctttgtaa aatatcacct ttcgcaaaat acaagcaaaa
     2041 aagaccgctt gtatttttta tttgtggttc gtaaacctcc cacacaaaaa aactaaggac
     2101 acaaaatgac tcatcaaaaa ccaaaagccg ttgtgatttt ttccggcggt caagactcta
     2161 ccacttgttt attccaagct attgctgaat ttggcaaaga aaatgtggaa gtcgttacct
     2221 ttcaatatgg gcaacgccat tcgattgaac ttgaaaaagc acgctggatt tctaacgatt
     2281 taggcgtcaa acaaacgctg attgatactt cggtgattaa agcgattact accaatgcgt
     2341 taatggataa taatgctgaa attcaacaac aaggcagcac acccaatacc tttgtggatg
     2401 ggcgtaatgc cttatttctg ctttataccg ccatttatgc caaagggcag ggcattcaaa
     2461 ctatttttac cggcgtgtgt gagaccgatt ttagtggtta ccctgattgc cgagatgtat
     2521 ttgtaaaatc aatgaatgta acgctaaatt tagcgatgga ctataatttc aacattcgca
     2581 cgccattgat gtatttaact aaaaaagaaa cttgggcatt agcagataag ctgggtgctt
     2641 ttgattatat tcgagaccat acccacacct gttatttagg cgttgaaggt ggttgccacg
     2701 actgcccgag ttgcgtattg cgagaaaaag gcttgaatga atatttagca gaaagggagg
     2761 taagcaatgt ttaaaattgc taaagaattt agttttgata tggctcatat gctagatgga
     2821 cacgatggca aatgccaaaa cttacacgga cacacctaca aattgcaagt tgaggtggca
     2881 ggtgagctat ttcaagaggg agctaaaaga ggaatggtaa tggactattc ggatctaaaa
     2941 gcagtggtaa aacaacatat tttagagccg atggatcacg cttttattta cgacttacag
     3001 agtgagcgtg aaagccaagt agcacaatta ttaattgatt taaactccaa ggtttatggt
     3061 atcccaactc gtaccacagc cgaagaaatg gcgaaatata tgtttgaaaa attagagaaa
     3121 gtgggcttac cggttagcct aattcgcttg tgggaaacgc ctacttctta ttgtgagtac
     3181 agcaaataat ggcggagtta atttcaaacc ctcgttttcc gattgttgag atctttgaaa
     3241 gcttacaagg ggaaggcttt aataccggaa tgccggctat ttttgtacgt tttggcaaat
     3301 gcaacctcac ttgcccgtgg tgcgatacca attacaatca atttgagcaa tggacgttaa
     3361 gcgatattct tgccaaagta agaagctatt ctgcgaaaaa tgtgattatt accggaggcg
     3421 agccaacgat tcagcctaat ttatcgttat tattagaaca atttaaaaaa gagggatatt
     3481 ttttagcgat cgaaaccaac ggcttaaaag aggttccggc acaaattgat tatattgcga
     3541 ccagcccaaa gcgaatgtac caagaaaagt atcaacgccg ttgcattgaa tttgcgaatg
     3601 aagtccgtat cgtcgttgat ggagaagtac agggtttctg tgagcaaata gaaagccaaa
     3661 ttaaagctga gcattactat ctttcacctt gtgaggtaga gggaaaaatg aacttgctgg
     3721 aaaccatcac ccagattggg ttattaaatc agcgtccaaa taagcctaaa tggcagctaa
     3781 gtatccaaac gcataaaatt gtggggattg aataagtaga agttagaaga tgatttgtaa
     3841 aagatcagac ttgatctgac aattcactct aaaaagtgag agtttcccgt ttagaatatg
     3901 agtgtccaaa
//
LOCUS       DANLXF010000214         3852 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.214, whole genome shotgun sequence.
ACCESSION   DANLXF010000214 DANLXF010000000
VERSION     DANLXF010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3852
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.214"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            185..544
                     /locus_tag="PXD66_002161"
     CDS             185..544
                     /locus_tag="PXD66_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320413.1"
                     /translation="MKTFSIHTSVWHDSSTKGFRHNIKHKRHDCWRADIRIKQKINNV
                     WVQISRIRKRFKCPEQAKSFAEQWKQTETPAFLQHQINRQALREELSNLEQRKIQKNG
                     MLEVIEYRISQIKELLK"
     gene            541..1020
                     /locus_tag="PXD66_002162"
     CDS             541..1020
                     /locus_tag="PXD66_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320414.1"
                     /translation="MKRNKVESYTIRLLKNNHTKRYFGEVILNGIKEISTAEMMSEIN
                     AIKALNKRIEALNKTKAVNLPNYPEIRGHQPIMYNYTFEQIEEDPEQVKPKPSKPKRK
                     PFTPYGLNGYFVDSNGNIRLHLDRRASAETITLETDFFNALAEMVKRTQEANNAKHA"
     gene            1154..1366
                     /locus_tag="PXD66_002163"
     CDS             1154..1366
                     /locus_tag="PXD66_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320415.1"
                     /translation="MIGYEDYTDASLQPEEDPSEEAFLLVEEKIIDVLENNGFETVAT
                     ILAGSDGWCNAINAYLAEQNKHRNEF"
     gene            1379..2302
                     /locus_tag="PXD66_002164"
     CDS             1379..2302
                     /locus_tag="PXD66_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HDL6320416.1"
                     /translation="MTNQNQQVAQKQDKFPIKTLFESPNVKQKLQELLNKNAATFTTS
                     VLQIVNSNSMLRNAEPMSVFNAACMAATLNLALQNGLGFAYIVPYQNRKEKKTEAQFQ
                     LGYKGLIQLAQRSGQFKRLVAVPVYEKQLLAEDPINGYEFDWKQKPAKDEKPIGYYAY
                     FKLINEFTAEIYMTTQEVHDHANRYSQTYRKGYGVWHDNFEAMALKTVMKLLLSKQAP
                     LSVEMQQAVLADQAVVKDVENAEFAYPDNEIQEAEFTELKVSDEVFEKCKQTIINKES
                     TLQDMCDNGYEFNKEQYAELEKLENEQQSNV"
     gene            2295..2957
                     /locus_tag="PXD66_002165"
     CDS             2295..2957
                     /locus_tag="PXD66_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017806850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation protein TolB precursor"
                     /protein_id="HDL6320417.1"
                     /translation="MYKFKPRCSMLHQLMSEPRTKADREAGLISDTAKAAVREVAKFD
                     LFGYQSFDGNKYTEKGNQLEEQAIKLSGFTRGLVLKKNTERRENDWISGECDIYVPSR
                     KLIIDTKCSWDIGTHPFFADEAIAKAEKAGYDIQMQGYMWLWDCEEAQIDFVLFPTPI
                     DLIGTYESTEKLIDLVEQIPQQKRITTVTVKRDEAIIAKIKDRVKAASKYYDKLIQEA
                     MN"
     gene            2998..3843
                     /locus_tag="PXD66_002166"
     CDS             2998..3843
                     /locus_tag="PXD66_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320418.1"
                     /translation="MTTQKKYELLQNDTINHNGRTLYRIKAITTFGTVFAGTLGGYIE
                     KESNLKHLGNAWVYDNAKVYDSAEVYGNAKVYDSAEVYDNAKVYDSAEVYGNAKVYDS
                     AEVYGNAKVYDSAEVYGNAEVYGNAEVWGSAKVWGNAEVWGSAEVYGNAKVYGNAEVW
                     GSAKVWGNAEVWGSAEVWGSAEVYGNAKVYDSAEVWGSAKVRSFAVLSERKMIFWASN
                     VGTENGTLTVYNGKDGLLVTRGCFTGTVEEFLAKSDEVHDEKTKREYQLLIEVAKSRI
                     LGEAT"
BASE COUNT         1356 a          824 c          851 g          821 t
ORIGIN      
        1 ataacaatga aaaagaatgt aaccaaaata aaataagaat tgatgtatac aacaaatgtt
       61 ccggaatgat gattgagctg gagggtattc ttacagaata caagctaact cttgatgagt
      121 gcgtagacga attcttagga aatgaccatt gtttgagtga atttattacc aaaggaacga
      181 aaaaatgaaa acttttagca ttcacactag tgtatggcac gacagctcaa ctaaagggtt
      241 tcgacacaac atcaaacata aacgccacga ctgctggaga gcggatatcc gcataaaaca
      301 aaaaatcaat aatgtgtggg tgcaaatctc aagaatacgc aagcgtttca aatgccctga
      361 acaagcaaaa tcctttgctg aacaatggaa acaaactgaa acacctgcat ttttgcaaca
      421 ccaaatcaac cgccaagcat taagagaaga attatcaaac cttgagcaac gtaaaatcca
      481 aaaaaacgga atgcttgagg tgattgagta tcgtattagc caaatcaagg agttactgaa
      541 atgaaacgca ataaagtaga aagctatact atccgcttat taaaaaataa tcatacaaaa
      601 cgctacttcg gagaagttat attaaacggc ataaaagaaa tcagcacggc agagatgatg
      661 tctgaaatta acgctattaa ggcattaaat aagcgtattg aagctctgaa taaaaccaaa
      721 gccgttaatt tgcctaatta tcctgaaatc aggggtcatc agcctattat gtacaattac
      781 acttttgaac aaattgagga agatcctgaa caggttaagc ctaaaccatc aaaaccaaaa
      841 cgcaaacctt tcacacccta cgggctaaac ggctactttg tggatagcaa cggcaatatt
      901 cgcctgcacc tagacagacg agcaagtgcc gaaacaatta cccttgaaac tgatttcttc
      961 aacgctcttg ccgaaatggt aaaacgtaca caagaggcaa acaatgctaa acatgcttag
     1021 acaatggatt acatacggct tatacgccct gctttttatg gttgccatca ccctcctaat
     1081 gcaatgaaga gcttatccct gaaagcacct gcaagcctga aacttgcgaa tttaaagtaa
     1141 cggagaaagt gtgatgatag gatacgaaga ctacacagat gcttcacttc aaccagaaga
     1201 agaccctagc gaagaggctt tcctcttagt ggaggagaaa attatagatg tgctagaaaa
     1261 caacggcttt gaaaccgttg ctactatcct agccggttct gatggctggt gtaacgcaat
     1321 taatgcctac ctggccgaac agaacaaaca cagaaatgaa ttttagagga ataagataat
     1381 gacaaaccaa aatcaacaag tagcacaaaa gcaagataaa ttcccgatta aaaccctatt
     1441 tgaaagcccg aatgtaaagc aaaaactaca agagctttta aacaaaaacg cagccacgtt
     1501 tacaacaagc gtgttgcaga ttgtgaatag taactcaatg ttacgcaatg ccgaaccgat
     1561 gagcgtattc aacgccgctt gtatggcagc aacgcttaat ttggcattac aaaacggctt
     1621 aggttttgcc tacattgtgc cgtaccaaaa ccgcaaagag aaaaaaaccg aagctcaatt
     1681 ccaacttggc tacaaaggtt taattcaact ggctcaacgt agcgggcaat ttaaacgcct
     1741 tgttgccgtg ccggtgtatg aaaaacaact gcttgccgaa gaccccatca acggttacga
     1801 gttcgattgg aaacaaaaac cggctaaaga tgaaaaaccg attggctatt acgcttattt
     1861 caaactgata aatgaattta ccgccgaaat ctatatgacc acgcaagaag tccacgacca
     1921 cgccaaccgc tacagccaaa cctaccgcaa aggctacggc gtatggcacg acaactttga
     1981 ggcaatggct ctcaaaactg tgatgaagct cttgctttcc aaacaagctc cattatcagt
     2041 ggaaatgcaa caagcggtct tggcagatca agcggtcgta aaagatgttg aaaatgcaga
     2101 atttgcctac cctgacaacg aaatccaaga ggcggaattt accgaactca aggtcagcga
     2161 tgaggtgttt gagaaatgca aacaaaccat catcaacaaa gaaagcacct tgcaagatat
     2221 gtgcgataac ggctacgagt tcaacaaaga gcaatatgcg gagttggaga agttagaaaa
     2281 tgaacagcaa tcaaatgtat aaattcaaac cccgttgctc aatgctccac caactaatga
     2341 gcgaacctcg caccaaagcc gaccgtgaag ccggactgat ttccgacacc gcaaaagcag
     2401 cggtgcgtga agtggctaaa tttgacctat tcggttatca atcctttgac ggcaacaaat
     2461 acactgaaaa aggcaatcag ctagaagaac aagccattaa gttaagcggt tttactcgtg
     2521 gcctggttct aaaaaagaac accgaacgcc gagaaaacga ttggatttca ggcgagtgtg
     2581 atatttatgt accaagccga aagctgatta tcgatacaaa atgcagttgg gacatcggca
     2641 cgcacccgtt ttttgcagac gaagccatcg ccaaagccga aaaagccgga tatgacattc
     2701 aaatgcaagg ctatatgtgg ctgtgggatt gcgaagaggc tcaaatcgat tttgtgttat
     2761 tccctactcc aatcgacctt atcggtactt acgaaagcac agaaaagctg attgaccttg
     2821 tggaacaaat cccccaacaa aaacgcatta ccaccgtgac ggttaagcgt gatgaagcca
     2881 tcattgccaa aatcaaagac agagtgaaag cagccagtaa gtattacgac aagcttatac
     2941 aagaagctat gaattaattc aatcgccccg aaaggggctt tttaataggt gacaaatatg
     3001 acaacccaaa agaaatacga actcctccaa aacgacacaa tcaaccacaa cggcagaaca
     3061 ctctaccgca tcaaagccat aaccacattt ggaacagtct ttgcaggtac acttggcggc
     3121 tatattgaaa aagagagcaa cctaaaacat ttaggaaatg cttgggtgta tgacaacgcc
     3181 aaagtgtatg acagcgccga agtgtatggc aacgccaaag tgtatgacag cgccgaagtg
     3241 tatgacaacg ccaaagtgta tgacagcgcc gaagtgtatg gcaacgccaa agtgtatgac
     3301 agcgccgaag tgtatggcaa cgccaaagtg tatgacagcg ccgaagtgta tggcaacgcc
     3361 gaagtgtatg gcaacgccga agtgtggggc agcgccaaag tgtggggcaa cgccgaagtg
     3421 tggggcagcg ccgaagtgta tggcaacgcc aaagtgtatg gcaacgccga agtgtggggc
     3481 agcgccaaag tgtggggcaa cgccgaagtg tggggcagcg ccgaagtgtg gggcagcgcc
     3541 gaagtgtatg gcaacgccaa agtgtatgac agcgccgaag tgtggggcag cgccaaagta
     3601 agatcatttg cagttctttc tgaaagaaaa atgattttct gggcgagcaa tgtcggcaca
     3661 gagaacggta cattaaccgt ttacaacgga aaagacggac tgcttgtaac aagaggctgt
     3721 ttcaccggca cagtcgaaga gtttttagca aaatccgacg aagttcacga tgagaaaacg
     3781 aagcgggaat atcagctact gattgaggta gcaaaaagca gaattttagg agaagcgact
     3841 taatcagtcg ct
//
LOCUS       DANLXF010000215         3832 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.215, whole genome shotgun sequence.
ACCESSION   DANLXF010000215 DANLXF010000000
VERSION     DANLXF010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.215"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            254..586
                     /locus_tag="PXD66_002167"
     CDS             254..586
                     /locus_tag="PXD66_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF413 domain-containing protein"
                     /protein_id="HDL6320419.1"
                     /translation="MADSFSVTRRFFDDKNYPRGFSRHGDYTIRESQTLEQFGQACLA
                     LESGERKPATQEEKRFVAVMKGEEVAETDIEKAWLKYRTLTSKTKRIYTLSGNAGSDA
                     GDDFSAAE"
     gene            651..1742
                     /gene="trmA"
                     /locus_tag="PXD66_002168"
     CDS             651..1742
                     /gene="trmA"
                     /locus_tag="PXD66_002168"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HDL6320420.1"
                     /translation="MTLPIEQYSDLLAKKTENLTALLKPFNPPAIEVFASKPSHFRMR
                     AEFRVWHEEGELYHIMFNPETKARYRVDSFPIASELINHMMTALLAEIKGNELLTRKL
                     FQIDYLSTLSGEIAVSMLYHKSLNEEWVEQANALKSRLEQQGFKVQIIGRANKQKIAL
                     DCDYVEEILPVHGKNYVYRQVENSFTQPNAEMNIKMLEWARNCTKNSEGDLLELYCGN
                     GNFSIALAENFRKVLATEISKSSVQSAQYNIEKNGIVNLQIIRMSAEEFTQAMNGVRA
                     FYRLKGIDLNAYECNTIFVDPPRAGLDQATLNMVQAYERILYISCNPETLAENLQQLS
                     QTHRIEKAALFDQFPYTHHIESGVWLIRK"
     gene            1834..1923
                     /locus_tag="PXD66_002169"
     tRNA            1834..1923
                     /locus_tag="PXD66_002169"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1868..1870,aa:Ser,seq:tga)
     gene            2079..2450
                     /locus_tag="PXD66_002170"
     CDS             2079..2450
                     /locus_tag="PXD66_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HDL6320421.1"
                     /translation="MKYLYIICGFICIGLGIIGAVLPGLPTTPFLLLALFCFTKGSER
                     IRQWFIQTKLYKKYLQEYDEKRAMTMKQKITILAISFPFTLFAFFALPHIGGKIALVV
                     LVIIQYWYFFCKMKTLEPENA"
     gene            complement(2447..3358)
                     /gene="xerC"
                     /locus_tag="PXD66_002171"
     CDS             complement(2447..3358)
                     /gene="xerC"
                     /locus_tag="PXD66_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDL6320422.1"
                     /translation="MTTSNPLFQQTRPYWDFLRIEKQVSPHTLSNYQRQLMAVSELFS
                     QAGIENWEEVESSSVRWMLSQSHKQGLSAKSIGLRLVALRQWFVYLIKIGRMSANPTL
                     GVKSPKVGKHLPKNIDAEQVGQLLAIESDEPSDIRDIAMMELMYSSGLRLSELQGLDL
                     GDMDLSAREVRVQGKGSKERIVPIGSQALKALNRWLEMRLQFKPQDNAVFLNKRGGRL
                     SHRSIQLAMKKWGERQGLESHLHPHKLRHSFATHMLEASGDLRAVQELLGHSSLSTTQ
                     IYTHLDFQHLAKIYDSAHPRARRKQED"
     gene            complement(3425..3715)
                     /locus_tag="PXD66_002172"
     CDS             complement(3425..3715)
                     /locus_tag="PXD66_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5377 domain-containing protein"
                     /protein_id="HDL6320423.1"
                     /translation="MSVKTEVLFSNHWNVRVSDPGFEGSPSHYFETVYLTLEAFINGE
                     NVHYEFTRKVEQEVKTHRSFTDVNELFEFLGEYLSPVALGNVGVRIGQLGLA"
BASE COUNT         1144 a          837 c          736 g         1115 t
ORIGIN      
        1 cacagtatat ctccaaatat taaagttaat caaaactcaa tacatttagg taatatacct
       61 taacaaatat aaaaaatata cctcagactg cattgtctat tttaaaagga tcaatatttc
      121 tccttaaaga gttttttgca aaaaatttgc caaatttgac cgctttttta cattaaggca
      181 ttgatttatt cgctgttttt gtttaatgta tgccgatttt attttcccta atttcaaaca
      241 acgaggcatt attatggcag acagtttcag cgttacgcgc cgcttttttg acgataaaaa
      301 ttatccacgt ggtttctctc gccacggcga ttacaccatt cgtgaatcac aaactttaga
      361 acaattcggt caagcttgct tggctttaga aagtggtgaa cgtaagccgg caactcaaga
      421 agaaaaacgt ttcgttgcgg taatgaaagg cgaagaggta gcagaaactg acattgaaaa
      481 agcgtggtta aaataccgca cattaactag taaaacaaaa cgcatctaca ccctttcagg
      541 caatgcaggt agcgatgccg gcgatgactt cagtgctgct gaataatttc taatttaaaa
      601 ccacgaagta attcgtggtt tgttttttca tttgctattt gtggtaaaaa atgactcttc
      661 ctatcgaaca atattccgat cttcttgcaa aaaaaaccga aaatttgacc gctttactca
      721 agccgtttaa cccgcctgcg attgaggtat ttgcctcaaa gccaagccat ttccgtatgc
      781 gggcagaatt tcgagtgtgg cacgaggaag gcgagctgta ccacattatg ttcaaccctg
      841 aaaccaaagc tcgttatcgt gtggatagct tccctattgc cagtgagctg attaaccata
      901 tgatgaccgc attattggcg gaaattaaag gcaatgaact actgactcgc aagctgttcc
      961 agatcgatta tttaagcacc ttaagcggtg aaattgcagt ctcaatgctt taccacaaaa
     1021 gcctgaatga agaatgggtt gaacaggcaa acgccttaaa aagccgttta gagcaacaag
     1081 gtttcaaagt gcaaattatc gggcgtgcca ataaacaaaa aatcgcactg gattgcgatt
     1141 atgtggaaga aattctaccg gtacacggca aaaattatgt gtatcgccaa gtggaaaaca
     1201 gctttaccca gccgaatgcg gaaatgaata ttaaaatgct ggaatgggcg agaaattgca
     1261 caaaaaatag tgaaggcgat ttgcttgagc tttactgcgg caacggcaat ttttcgattg
     1321 ccttggcgga aaacttccgc aaagtgctgg caaccgagat ctcaaaatct tcagtgcaat
     1381 cggctcaata caatattgaa aaaaatggca ttgttaacct acaaattatc cgaatgtcgg
     1441 cagaagaatt tacccaagcg atgaacggtg tgcgagcctt ttatcgctta aaagggattg
     1501 atttaaacgc ctacgaatgc aataccattt ttgttgatcc acctcgtgca gggttagatc
     1561 aagctacatt aaatatggtg caagcgtatg agcggattct ctatatctct tgcaatccgg
     1621 aaacccttgc cgagaattta caacaattaa gccaaaccca tcggattgaa aaagccgctc
     1681 tttttgacca attcccttac acccaccata ttgaaagtgg cgtttggtta attagaaaat
     1741 aaattgtgct gaaaaaaaga acgaataagc agcttttaag atttattact tgacgatgtt
     1801 cgcaataatc tctattattg caacgttttc aacggaggaa tggcagagcg gttgaatgca
     1861 ccggtcttga aaaccggcga gggttaacgc cctccgtgag ttcgaatctc acttcctccg
     1921 ccagtcttta aacctgttga gcaatcagca ggttttttta tttcatttgc aagaaaatca
     1981 gataaaacca ccgcttgaat ccatcgctat aaactggttg aaatcagcct aaagttgctt
     2041 taggataatt gcgattcatt atcaataact taattattat gaaatacctt tatattatct
     2101 gtggttttat ttgtattggt ttaggcatca tcggggcagt attaccgggc ttgccgacca
     2161 caccgttctt acttttggct ctgttctgct ttactaaagg ctcggagaga atccgccagt
     2221 ggtttattca aaccaaatta tacaaaaaat atctgcaaga atatgatgag aaacgggcaa
     2281 tgacgatgaa gcaaaaaatc acgattttag cgatttcgtt tccgtttaca ctatttgctt
     2341 tctttgcact gccccatatc ggtgggaaaa ttgcgttagt agtgttagtg ataattcaat
     2401 attggtattt cttctgcaaa atgaaaaccc ttgaaccgga gaatgcctaa tcttcctgct
     2461 tccgccttgc tcttgggtgg gcagagtcat aaattttagc taagtgttgg aaatccagat
     2521 gcgtataaat ttgagtggtg gacaggctac tatgacccaa taattcctga accgctcgca
     2581 aatcaccgct tgcctctaac atatgagtcg caaaagagtg acgtaattta tgtggatgca
     2641 gatggctttc cagcccttgt cgctcacccc atttcttcat tgccaactga atggaacgat
     2701 gagataaccg cccacctcgc ttattcagaa acaccgcatt atcttgcggc ttaaattgca
     2761 aacgcatttc taaccagcga tttaacgctt ttaacgcttg cgagccaatc ggcacaattc
     2821 gctctttgct acccttacct tgcacccgta cttctcgtgc cgataaatcc atatcgccta
     2881 aatccagccc ttgtaattcc gataaacgca agccggaact atacatcagc tccatcatcg
     2941 caatatctcg aatatcgctc ggctcatcac tctcaatcgc cagcaactgc ccgacctgct
     3001 ccgcatcaat atttttcggc aaatgcttac ctactttcgg cgatttcacc cctaaagtcg
     3061 ggtttgccga cattcgcccg attttaatca aatagacaaa ccattgccgt aacgccacca
     3121 agcgtaagcc gatacttttc gcactcaacc cttgcttatg gctttggctt aacatccaac
     3181 gcaccgagct actttcgacc tcttcccaat tttcaatgcc tgcttgcgaa aaaagctcgc
     3241 tcaccgccat taattggcgt tgataattgc ttaaggtatg cgggctaact tgcttttcaa
     3301 tgcgtaagaa atcccaatag gggcgggttt gttggaaaag agggttggaa gtggtcataa
     3361 acgtaataac aagcggttaa tttttaggaa aatgttgcaa gggcaaaaga tttgcccttt
     3421 gagattaggc tagccccaac tgaccgatac gcacgccaac attaccaagt gcaacagggc
     3481 ttaaatattc gccgagaaat tcaaacaatt cattcacatc agtaaaactg cgatgagttt
     3541 tcacttcttg ctccacttta cgagtaaatt cataatgtac attttcgccg ttaataaagg
     3601 cttccagcgt taaatatacg gtttcaaaat agtggcttgg cgagccttca aagcccggat
     3661 cactcactcg aacattccaa tgattactaa ataaaacttc ggtttttact gacattgtgt
     3721 tttccttctt cctataagta cgtacattat tttaatcaag atagattgaa tttgccactt
     3781 ttcctagtca gaatcgaggc aaatcttatt tggtttgtcc tcttatatct tt
//
LOCUS       DANLXF010000216         3813 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.216, whole genome shotgun sequence.
ACCESSION   DANLXF010000216 DANLXF010000000
VERSION     DANLXF010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.216"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(89..1024)
                     /gene="ftsX"
                     /locus_tag="PXD66_002173"
     CDS             complement(89..1024)
                     /gene="ftsX"
                     /locus_tag="PXD66_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HDL6320424.1"
                     /translation="MIRSFSKLGAQTNYSLRAVWQDLMQRKLGTFLTVLVIAVSLTIP
                     TVSYLLWKNTHEAATQFYPEPQLTVYLHKNLAEHDVNTVVDRIRAFETDKIESFNYIS
                     RQQSLDEFRSWSGFGEALDILDENPLPAVVTLKPKANFNNADTMVELRNGLQQIKGVQ
                     EVRLDNGWLEKLTALTWLIARIAVVCTLLMLLAVFLVIGNSVRTDVANSKAAIEVQQI
                     LGATDHFIARPFLYTGMIYGLFGSLLAVLFSAVTISYFTGVVKYVTDMFTVQFELNGL
                     DFSEAFFLVVLCVFIGWLSAKIATMRNIFRIGSRG"
     gene            complement(1034..1687)
                     /gene="ftsE"
                     /locus_tag="PXD66_002174"
     CDS             complement(1034..1687)
                     /gene="ftsE"
                     /locus_tag="PXD66_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDL6320425.1"
                     /translation="MIKFTNVNKVYKGGKPALQGIDFHLPAGGMAYITGHSGAGKSTL
                     LKLIMGIERANGGQILFNGHDITRLAHHELPFLRRQIGMVHQDYRLLTDRTILDNVAL
                     PLIILGMNQAIVEREARIALERVGLADKANYLPLHLSGGEQQRVDIARAIVHRPTLLL
                     ADEPTGNLDEKLSFEIFRLFEEFNQAGTTVLIATHDTNIISKRPKPCLVLEQGHLKF"
     gene            complement(1680..3176)
                     /gene="ftsY"
                     /locus_tag="PXD66_002175"
     CDS             complement(1680..3176)
                     /gene="ftsY"
                     /locus_tag="PXD66_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDL6320426.1"
                     /translation="MSEKKKGFWSWLGFGKKEEAEQKEIQQEQESIQPEQPVIVEPET
                     EKSFSEKLDELEDKIEEKVDQLEDYFEEKEEKIEQFAEQVEDTIEQKVEDFKEFVEEK
                     VEEAKAFFDDVEDKIEQTFEEQKGEQAVENLQNIATEQLDLNEQEQVTYQVEEAVAEE
                     VAETELVNNVQDTVEESTIQDEYQEKPSQGGFFSRLIQGLIKTKQNIGSGFRNFFRGK
                     KIDDELFEELEEQLLVADLGMPTTQKIINNLTQHATKQQLKDADLLYQQLKVELGEVL
                     KPVAQPLVLEAKKPYVILMVGVNGVGKTTTIGKLARKFQQEGKSVMLAAGDTFRAAAV
                     EQLQVWGERNNIPVVAQSTGSDSASVIFDAMQSAAAKGIDVLIADTAGRLQNKNNLMD
                     ELKKIVRVMKKYDETAPHEIMLTLDAGTGQNAISQAKLFNEAVGLTGITLTKLDGTAK
                     GGVIFAIADQFNIPIRFIGVGEKIEDLRPFNAEEFIEALFSHEDEQND"
     gene            3255..>3813
                     /gene="rsmD"
                     /locus_tag="PXD66_002176"
     CDS             3255..>3813
                     /gene="rsmD"
                     /locus_tag="PXD66_002176"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDL6320427.1"
                     /translation="MKKNRNSPQSVQQMGEVRVIAGLWRGRKLPVLNAEGLRPTTDRV
                     KETLFNWLMHDIAHSRCLDCFAGSGSLGIEALSRQAQAVVFLEKFANAANQLKKNLQA
                     LKSEQGSVIHTDTLAYLAQKNNGEPFNIVFIDPPFHQGFVPQVLNLLAENNWLAPNAI
                     LYVETEKNHPPLELPQGWQILKEKAA"
BASE COUNT         1065 a          922 c          688 g         1138 t
ORIGIN      
        1 agcaataata tattgcttat agtaaaaaac caagttccta ggaactggtt tttatctgca
       61 attatttctc taaaaacaac cgcttgtatt agcctctact accaatacgg aaaatatttc
      121 gcattgtcgc aatttttgcc gacagccagc caataaacac acaaagcacc actaagaaga
      181 aggcttcgct gaaatctaat ccgttcagtt caaattgtac ggtaaacata tcagtcacat
      241 acttcactac accggtgaaa tagctaatcg ttaccgcact aaacagcacc gctaacaggc
      301 tgccaaacaa gccgtaaatc atacctgtgt ataaaaacgg acgggcaata aagtgatcag
      361 ttgcccctaa aatttgttgg acttcaatcg ctgctttgct attggcaaca tcggttcgca
      421 cactgtttcc aatcactaaa aaaactgcca acagcattaa taaggtacac accaccgcaa
      481 tacgagcaat cagccaagtt agagcagtta atttttccag ccagccgtta tctaaacgaa
      541 cctcctgcac ccctttaatt tgttgcaaac cgttgcgtag ctccaccatc gtatcagcat
      601 tattaaaatt tgctttcggt tttaaggtaa cgaccgcagg caacgggttt tcatctaaaa
      661 tatctaacgc ttcaccaaag cctgaccaag aacgaaattc gtctaaactt tgttggcgag
      721 aaatatagtt aaatgactca attttatccg tctcaaaagc acgaatgcga tcaacaacgg
      781 tattcacatc gtgttctgcc aaatttttgt gcagataaac ggttaattgt ggctcagggt
      841 aaaattgtgt tgctgcctcg tgggtattct tccaaagtaa ataactcacg gttggaatcg
      901 taagtgagac cgcaatcacc agcaccgtca gaaaagtgcc gagcttgcgt tgcattaaat
      961 cttgccaaac cgctcttaaa gagtagttgg tttgagcacc taatttacta aatgaacgaa
     1021 tcataatatt tccttaaaat ttaagatgcc cttgctccag cactaaacac ggctttggac
     1081 gctttgaaat aatattggta tcgtgggtgg caatcagcac cgttgtgccg gcttgattaa
     1141 attcctcaaa caaacggaaa atttcaaacg agagcttttc atctaagttt ccggttggct
     1201 catcggctaa taacagcgtt gggcgatgaa caatggccct ggcaatatcg acacgttgtt
     1261 gctcaccgcc ggaaaggtgc aacggcagat agttcgcttt atctgccaag ccaacacgct
     1321 caagggcaat gcgggcttca cgctccacaa tcgcttggtt cattcccaaa ataatcaatg
     1381 gtaatgcgac attatccaaa atggttcggt cggttaataa gcgataatct tgatgcacca
     1441 tcccgatttg gcggcgtaaa aacggcaatt catgatgagc aagtcgggta atatcgtgtc
     1501 cgttaaacaa aatttgcccg ccattggcac gctcaatccc cataattaat tttaatagcg
     1561 tgcttttccc cgcaccggaa tgcccggtaa tatatgccat tccaccggca ggtaggtgga
     1621 aatcaatgcc ctgtaaggct ggctttccgc ccttataaac cttgttcaca ttagtgaatt
     1681 taatcattct gttcgtcctc gtggctaaat aaggcttcaa taaattcttc tgcattaaac
     1741 gggcgtagat cttcaatttt ctcgcccacc ccaataaagc ggatcggaat attgaattga
     1801 tcggcaatcg caaaaatcac gccacctttt gccgtaccgt ctaatttagt taaggtgata
     1861 ccggttaaac ccactgcctc attaaacaat ttcgcttggc tgatcgcatt ttgcccagtg
     1921 ccggcatcaa gcgtaagcat aatttcgtgc ggtgcggttt catcatattt tttcatcacc
     1981 cgcacaattt tcttcaactc gtccatcagg ttatttttgt tttgcaaacg ccctgcagta
     2041 tcagcgatta gcacgtcaat cccttttgcc gcagcggatt gcatcgcatc aaaaatcacc
     2101 gaggcagaat ccgaaccggt gctttgtgcc accaccggaa tgttatttcg ctctccccaa
     2161 acctgtagct gttcaactgc cgccgctcgg aacgtgtctc ctgctgctaa cattaccgat
     2221 ttgccttctt gttggaattt acgggcaagt ttgccaatgg tagtggtttt ccccacgcca
     2281 ttcacaccaa ccattaaaat gacatacggt tttttcgctt ccaacactaa cggttgagcc
     2341 accggtttta gcacttcacc cagttccact ttcaactgtt gatacaacaa atctgcatct
     2401 tttaattgtt gcttggtagc atgttgcgtt aaattgttga tgattttttg tgtggtcggc
     2461 atacctaaat ccgccacaag caactgctct tccagctctt caaataactc atcatcaatt
     2521 ttcttgccac ggaagaaatt acggaagccc gaaccaatat tctgcttggt tttaatcaag
     2581 ccttgaatta aacgactgaa gaaaccgcct tgactcggtt tttcttgata ttcatcttgg
     2641 atagtagatt cttcaactgt gtcttgaaca ttatttacta attcagtttc agcaacttct
     2701 tcagccacag cctcttcgac ttgataagta acttgctctt gttcgttgag atctaactgc
     2761 tcagttgcaa tattttgaag attttcgacc gcttgttcgc ctttttgttc ttcaaatgtt
     2821 tgttcgattt tatcttcaac atcatcaaag aacgctttcg cctcttcaac tttttcttca
     2881 acaaactctt tgaaatcttc gactttttgt tcaatggtat cttctacttg ctcagcaaat
     2941 tgctcaattt tttcttcttt ttcctcaaaa tagtcttcta actgatctac cttttcttca
     3001 attttatctt ccagctcatc taatttttca gaaaacgact tctcagtttc cggttcaaca
     3061 ataacaggct gttccggttg aatactctct tgttcttgtt gaatctcttt ctgttcagct
     3121 tcttctttct taccaaatcc aagccaagac caaaaccctt ttttcttctc tgacatattt
     3181 ttctctcatt tctcgtaaaa atagactaaa cttacatagt ctatcaaaaa atcccttatt
     3241 ttcctttagt gagtatgaaa aaaaatcgaa attcccccca atctgttcag caaatgggcg
     3301 aagtacgggt tatcgccgga ctgtggcgag gcagaaaatt acctgtacta aatgccgagg
     3361 ggcttcgccc gacaaccgat agagtaaaag aaacgctgtt taactggtta atgcacgaca
     3421 ttgctcactc acgctgtctt gattgctttg caggcagtgg ctctctgggc attgaagccc
     3481 tttcccgcca agcacaagcg gtcgtttttt tggaaaaatt tgcaaatgct gccaaccagt
     3541 taaagaaaaa tctccaagca ttaaaatccg aacaaggtag cgttattcac actgacaccc
     3601 tcgcctattt agcacaaaaa aataacggtg agccatttaa catcgtattt atcgatccgc
     3661 cttttcatca agggtttgta cctcaagtgc taaacttgct ggcagaaaat aactggctcg
     3721 ccccgaatgc gattttgtat gtggaaaccg agaaaaatca cccgccatta gagctaccgc
     3781 aaggctggca aattttaaag gaaaaagcag ccg
//
LOCUS       DANLXF010000217         3777 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.217, whole genome shotgun sequence.
ACCESSION   DANLXF010000217 DANLXF010000000
VERSION     DANLXF010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.217"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..1273)
                     /locus_tag="PXD66_002177"
     CDS             complement(<1..1273)
                     /locus_tag="PXD66_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HDL6320428.1"
                     /translation="MKTLTLTANKITAVEGAAIGANQGSFAQKLNSTKARIDQLNAYR
                     IERAINANNHHFMHVFSLIPLLIHLNHPVLPAYVENAPQGIWHFQLSDYQKQFLVSYR
                     FDEAISADHLGPHPSFDALYSMGSTGTITQTSCSDLDLWLCYPNHFTIEQYKLMELKI
                     NKLQEWAKTFQIDINIYLMNPEQFKSQTYNSSVTDEHSGSAQHFFLLDEFYRSAILLA
                     GKRLLWLHLHKNEYQNVHNNPEIDLTEWIDFGDFSSLSTSEFFGASLWQLYKGINNPY
                     KSAIKILLLECYAHTYPKTKLISKEFKKKLLSDNALEYHFDPYLAMLELVTEHLRSRK
                     EWVKLDRLRVCFYAKAVEGEINNNWRSRDLDELVNQWSWSEKQIQLLQNRQHWKIKQV
                     MNHENMLIDHLLQSYRNLIHFARKFHINPSIM"
     gene            1534..2751
                     /gene="fabB"
                     /locus_tag="PXD66_002178"
     CDS             1534..2751
                     /gene="fabB"
                     /locus_tag="PXD66_002178"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HDL6320429.1"
                     /translation="MKRVVITGLGVISSIGNNKEEVLESLKAGKSGIEFVPEFAEVGM
                     RSQVAGTIKLNPAELIDRKIYRFMGDAAAYAYLSMKEAIEDSGLTEEQVSNDRTGLVI
                     GAGTGSAHSQLVACDAVRGPRGVKAVGPYAVTKTMASSVSACLATPYKIRGVNYSISS
                     ACATSAHCIGHAMELIQLGKQDVVFAGGAEELSWECATEFDAMGAVSTKYNETPTKAS
                     RAYDANRDGFVIAGGGAVVVVEELEHALARGAKIYAEIVGYGATSDGYDMVAPSGEGA
                     ERCMRQAMATVNGEIEYINVHGTSTPVGDVKELGAIRNVFGDKTPAISSTKSMTGHSL
                     GAAGAHEAIYSLLMLDNNFIAPSINIETLDEQAQGMNIVTERQDKALTTVMSNSFGFG
                     GTNACLVFQRYSK"
     gene            2869..3015
                     /locus_tag="PXD66_002179"
     CDS             2869..3015
                     /locus_tag="PXD66_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320430.1"
                     /translation="MIKPLDSVYWRGKDVSEFFARKRLPDTAVQCVKDNELSYVYGYS
                     TSFV"
     gene            2984..3694
                     /locus_tag="PXD66_002180"
     CDS             2984..3694
                     /locus_tag="PXD66_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HDL6320431.1"
                     /translation="MFMVIQLALYNLQTYEVGRNATTIFMTTDKVYTGGSTWTYLFAD
                     ADNKITSVREMNGFGDVEKEFKCGEYKEAVTIASKEDLAKRKTQPRLWSSIYMSPEQN
                     MMWVTDNTSKNLENAKQDAFKQCEKNGGRNCQMILSFSNMCLAFAQGYKDGQGFEDFG
                     YGIVSSLAEENALENCNQKGGSSCLLSAPAVCAIPCDILSNNKCMYDQPQIIIPGKNN
                     NQPINPPANGNGEHWLSN"
BASE COUNT         1162 a          616 c          819 g         1180 t
ORIGIN      
        1 tcataatgct cggattaata tgaaattttc gagcaaaatg aattaaattt cgataacttt
       61 gtagtaaatg atcaatcagc atattctcat gattcatcac ttgcttaatt ttccaatgtt
      121 gacggttttg aagtaattga atttgttttt ctgaccaaga ccattggtta accagctcgt
      181 ctaaatcacg acttctccag ttgttattta tttctccctc aaccgctttt gcataaaaac
      241 agactcgcaa gcggtctaat ttcacccact ctttgcgtga tcttaagtgt tctgttacca
      301 attcaagcat ggctaagtag ggatcaaagt gatattccaa agcattgtca cttagtagtt
      361 ttttcttaaa ttctttggag atgagctttg ttttaggata agtgtgagcg tagcattcga
      421 gcaataaaat tttaattgcc gatttataag ggttattaat tcctttgtag agctgccata
      481 agcttgcacc aaaaaattca ctggttgaaa ggctggaaaa atcgccgaaa tcaatccatt
      541 ccgttaagtc aatttcaggg ttattgtgaa cattttggta ttcattttta tgtaaatgca
      601 accagagtaa acgcttaccg gcaagtaaaa tagctgaacg ataaaattca tctaatagaa
      661 aaaaatgctg tgctgaacca ctgtgttcat cggttacgct actgttataa gtttggctct
      721 taaattgttc tgggttcatt aaataaatat tgatgtcgat ttggaatgtc tttgcccatt
      781 cttgcaattt attaattttg agctccatta acttatattg ttcaattgta aaatggttcg
      841 gatagcatag ccataaatct aaatcagaac agcttgtttg ggtaatagta ccggtgctac
      901 ccatcgaata aagagcatca aagctgggat gggggcccaa atgatcggca gagattgctt
      961 catcaaaacg gtaagaaacc agaaattgtt tttgataatc agagagttga aaatgccaaa
     1021 tcccttgtgg tgcattttca acataagccg gtaacacagg gtgattaaga tgaataagca
     1081 atggaatgag agagaaaaca tgcataaaat gatgattatt agcattgata gctcgttcta
     1141 ttcggtatgc attaagttga tcaattcgtg ctttagtaga gtttaatttt tgggcgaagc
     1201 tcccctggtt cgccccaatg gcagcgcctt ctacggctgt tattttgttg gctgtcaggg
     1261 ttaatgtttt cacgtcagct ttccttacta aagtgtgaac tgtttagcat tttagcggaa
     1321 aagtgattat tatgtaatag ggtaaaaaat cgggaaatta aatttcattc cctattttgc
     1381 aaaaaatgtc tgaaattcaa ccgctatctt gatagaaaga gttcagacca gtaatgaaat
     1441 tgtgtaaaat aatcaagttt tagtagctta tttgagtgaa caagtgtaag ctgaatcaag
     1501 tatttttcag atcaaaatga tgaaggaaac aaaatgaaaa gagtcgttat tacaggatta
     1561 ggtgtaattt ccagtatcgg taacaacaaa gaagaagtgt tggaatcact aaaagcaggt
     1621 aaatcaggca ttgaatttgt gcctgagttt gctgaagtgg gtatgcgtag ccaagtggca
     1681 ggtacgatta aattaaatcc ggctgagtta attgaccgta aaatctatcg ttttatggga
     1741 gatgctgcgg cttacgctta tctttcaatg aaagaagcaa tcgaagactc aggcttaacc
     1801 gaagaacaag tttcaaacga tcgtaccggt ttggtgatcg gagctggtac aggctcggcg
     1861 cacagtcaac ttgtagcttg tgatgcagta cgtggcccac gtggtgtgaa agccgttggg
     1921 ccttacgcag taaccaaaac aatggcatca agtgtgtcag catgcttggc aacaccttat
     1981 aaaattcgtg gtgtaaacta ttcaatcagc tcagcttgtg cgacttcagc acactgtatt
     2041 ggacacgcga tggagttaat ccaattaggt aaacaagatg tggtgtttgc aggtggtgca
     2101 gaagaacttt cttgggaatg tgcaaccgag tttgatgcaa tgggtgctgt ttcaactaaa
     2161 tataatgaaa caccaacaaa agctagccgt gcttatgatg ctaatcgtga cggttttgtg
     2221 attgccggtg gtggtgcagt ggttgtggtg gaagagttag aacacgctct tgctcgtggt
     2281 gccaaaatct atgctgaaat tgtgggctat ggggcaacct cagacggtta cgatatggtc
     2341 gcgccaagtg gtgaaggtgc tgagcgttgt atgagacagg cgatggcaac tgtaaatggt
     2401 gaaatcgagt atattaacgt acacggcact tctactccag tgggtgatgt aaaagagtta
     2461 ggggcgatcc gtaatgtctt tggtgataaa acgccagcaa tttcatcaac caaatcaatg
     2521 accggacact cattaggtgc agcaggtgcg cacgaggcaa tttattcatt attaatgtta
     2581 gataataatt ttattgcacc aagcattaat attgaaaccc ttgatgaaca agcacaaggt
     2641 atgaatattg tgactgaacg ccaagacaaa gcattaacaa ccgtgatgtc aaacagcttc
     2701 ggctttggtg gtactaatgc gtgtttagtg ttccaacgct attctaagta acataaaaag
     2761 agcaagtgaa agcttgctct tttttctttt gaggtaatta ttataaattt caaaataagt
     2821 tctttgctat ttgttatttt gtcattattt ttgaccgctt gttctacaat gattaagcca
     2881 ctagatagtg tttattggcg tggtaaagat gtatctgaat tttttgcacg taaacgttta
     2941 cctgatacag cggttcaatg cgtaaaagat aatgaattaa gctatgttta tggttattca
     3001 actagctttg tataatttgc aaacttatga agttggtcgt aatgcaacca ctatatttat
     3061 gacaacagat aaggtttata caggtggatc gacttggaca tatctttttg ctgacgctga
     3121 taataaaatc acttctgttc gtgaaatgaa tggttttggt gatgtcgaaa aagagtttaa
     3181 atgtggtgaa tataaagaag cggtaaccat tgccagtaaa gaggacttag caaagcgtaa
     3241 aacacagcct cgtttatggt catcaattta tatgtcacct gaacaaaata tgatgtgggt
     3301 gactgataat acgtctaaaa atttagaaaa tgcgaagcaa gatgcgttta aacagtgtga
     3361 aaagaacggt ggtcgtaatt gccaaatgat cttatcgttt agtaatatgt gccttgcttt
     3421 tgcacaaggt tataaagatg gtcaaggctt cgaggatttt gggtatggta tcgttagtag
     3481 tcttgctgaa gaaaatgcat tagagaattg taatcaaaaa ggtggttcaa gttgtcttct
     3541 ttctgcccct gccgtatgtg ctattccctg tgatatattg tcgaacaaca aatgtatgta
     3601 cgatcagcct caaattatta ttccaggtaa gaataataac caaccgatta atccaccagc
     3661 taatggcaac ggtgagcatt ggttgagtaa ttaaatcatt ttagtaaaaa gtgcggaaac
     3721 gcactttttt attgcctgca agcggtcatt tttagtgaaa actttgctaa aaatctt
//
LOCUS       DANLXF010000218         3774 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.218, whole genome shotgun sequence.
ACCESSION   DANLXF010000218 DANLXF010000000
VERSION     DANLXF010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.218"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..1943)
                     /locus_tag="PXD66_002181"
     CDS             complement(<1..1943)
                     /locus_tag="PXD66_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Met) cytidine acetyltransferase"
                     /protein_id="HDL6320432.1"
                     /translation="MRTIAIITSPNNADFIQQGYFYIPENHQLKAIPFSNAKTILGQE
                     YPFAIYTMQAENGVNFNLDAFAILAGTIQENGTLFLLCPQWDNLENELDFDALRWNEN
                     QAIACPNFYQHFKQLVAKFGFEVRTNLPKLPATSGQIPSKIYQLTQEQQNILQNLPLD
                     PADIHLITAPRGRGKSTLAGRLAKALPAQNKVLITARSYSALPSFWRSAEQKIPFFAP
                     DNLIEQVKSKNISPNQWLFIDEAASLPLPILHTLCAYFEKVVLTTTTQNYEGTGRGFE
                     LKFPVMLDKPFKHWTLSQPLRWSENDPLEAFVTELLLLSEEKSPLFSKEGKGEIYSLR
                     SHYVPLLKQRSNAKRLWQKQLSKNISYSNKHLQEKIAFYHLLANAHYKTTPTDLRRLF
                     DGENQLLFSQQENEQLIGGIWAMQEGGLDEKLTQAIWLGERRPQGSLVAQYLCFQGNL
                     PQACKLHSIRISRIAVLPEKQNQGVGKRLISQFILQNSVEKRPLDFISVSFGLTTPLH
                     AFWQSCGFQLVQITPNKEASSGYQSAMMIYPISEQGKLFAQQATQAFARDFALQPFFT
                     EICEELQNFEQFRPLATHNLDERDWKNLTAFAHGKRTFSAAYVSLKRLYLSDKARFSE
                     LDIFERKGQKISKMEVENYRKLV"
     gene            complement(2021..2503)
                     /gene="ybeY"
                     /locus_tag="PXD66_002182"
     CDS             complement(2021..2503)
                     /gene="ybeY"
                     /locus_tag="PXD66_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDL6320433.1"
                     /translation="MENLYIDLQIACENTENLPSEQQFYTWVQKALAVEAKTDDFPES
                     EITIRIVDEAESHELNLTYRGKDKPTNVLSFPFEAPEGIEMPLLGDLVICRQVMEKEA
                     EEQGISAESHWAHLAIHGTLHLLGYDHIEEDEAVEMESLETEIMQSLGYEDPYLSEKI
                     "
     gene            complement(2536..2991)
                     /gene="moaE"
                     /locus_tag="PXD66_002183"
     CDS             complement(2536..2991)
                     /gene="moaE"
                     /locus_tag="PXD66_002183"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HDL6320434.1"
                     /translation="MFETLIEVQEAEFDQNRIYQWLSEQHSVGAATLFIGKVREMNLG
                     DNVSGLFLEHYPAMTKKALQEIVAQARTRWDLQRVAVIHRIGQLHTGDEIVLVGVSSA
                     HRGDAYHANEFIMDYLKTKAPFWKREQTQAGERWIEGRESDQQAAKKWQ"
     gene            complement(2993..3238)
                     /gene="moaD"
                     /locus_tag="PXD66_002184"
     CDS             complement(2993..3238)
                     /gene="moaD"
                     /locus_tag="PXD66_002184"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005631889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HDL6320435.1"
                     /translation="MIKILFFAQVRELVATDRLDVEPTFTTAEALREHLSQQGKKWQL
                     ALEKGKLLVAINQTICPLETEIKDGDEVAFFPPVTGG"
     gene            complement(3231..3707)
                     /gene="moaC"
                     /locus_tag="PXD66_002185"
     CDS             complement(3231..3707)
                     /gene="moaC"
                     /locus_tag="PXD66_002185"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDL6320436.1"
                     /translation="MTTFTHINQNGEANMVDVSMKQETVRIARAEAFVRMNAETLQMI
                     VSGNHHKGDVFATARIAGIQAAKRTWELIPLCHPLLLSKVEVQLEALPESNQVRIESL
                     CKLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMIIENVRLLSKSGGKSGDFKVD"
BASE COUNT          986 a          818 c          792 g         1178 t
ORIGIN      
        1 aaaacgagtt ttcgataatt ttccacctcc attttggaga ttttttgccc ttttcgttca
       61 aaaatatcca gctctgaaaa gcgtgcttta tccgataaat aaagccgttt taaactcaca
      121 tacgccgctg aaaacgtccg cttgccatga gcaaacgcgg ttaaattttt ccaatcacgt
      181 tcatctaaat tgtgtgtggc aagcggtcga aattgttcga aattttgcaa ttcctcacaa
      241 atttccgtga aaaatggctg aagggcgaaa tctctggcaa aggcttgggt tgcctgctgt
      301 gcaaataact ttccttgctc cgaaatcggg taaatcatca ttgcactttg ataaccactg
      361 cttgcctctt tattcggtgt gatttgcaca agttggaaac cacaagattg ccaaaatgcg
      421 tggagtggag tagttagccc aaagctcaca gagataaaat caagcggtcg tttttctacc
      481 gaattttgca aaataaactg agaaatcaac cgcttgccaa cgccttgatt ttgcttttca
      541 ggcaataccg caatccgaga aatgcgaatg gaatgcaatt tgcaagcctg cggtaaattc
      601 ccttgaaaac agagatattg agccactaga ctgccttgcg gacggcgttc ccctagccaa
      661 atggcttggg ttaatttttc atctaaacca ccctcttgca ttgcccaaat tccgccgata
      721 agttgctcgt tttcctgttg tgaaaacaaa agctgatttt cgccatcaaa taaacggcgt
      781 aagtcagttg gagtggtttt gtaatgggcg ttggcgagta agtggtagaa agcgattttt
      841 tcctgtaaat gcttattcga ataacttata ttcttcgata attgcttctg ccacaaacgc
      901 tttgcgtttg aacgttgctt tagcaacggc acatagtgtg agcgaagcga ataaatctcc
      961 cccttcccct ctttgctaaa gaggggagat ttttcttcgg aaagtaatag caactcagtc
     1021 acaaatgctt cgagaggatc attttcgctc caccgcaatg gttgagataa cgtccaatgt
     1081 ttaaacggtt tatccaacat taccgggaat ttcagctcaa atcctcgccc cgtgccttcg
     1141 taattttggg ttgttgtggt taaaaccact ttttcaaaat aggcacaaag tgtgtggagt
     1201 atcggcaagg gtaggctagc tgcctcatca ataaagagcc attggttagg tgaaatgttt
     1261 ttacttttaa cttgctcgat taaattgtca ggtgcgaaaa aagggatttt ttgctctgcc
     1321 gaacgccaaa aactgggcaa tgcagaataa gaccgtgcgg taattaatac cttattttgt
     1381 gcgggtaacg cttttgcaag tcgccctgca agggtagatt tgccccgccc acgaggggcg
     1441 gtaatcaaat gaatatcggc aggatcaagc ggtagatttt gcaaaatatt ttgctgttct
     1501 tgcgttaatt ggtaaatttt tgaaggaatt tgaccgcttg tagccggtaa ttttggcaaa
     1561 ttagttctga cttcaaaacc aaatttagcg actaattgtt taaaatgttg atagaaattc
     1621 gggcaggcaa tagcctgatt ttcgttccaa cgtaacgcat cgaaatctaa ttcattttcc
     1681 aaattatccc actgaggaca aagcaaaaat aaagtaccgt tttcttgaat agtgccggca
     1741 agtattgcaa aagcatctaa gttaaaattc accccatttt ctgcttgcat agtataaatg
     1801 gcaaaagggt attcttgtcc taaaatcgtt ttggcattgg aaaaggggat agctttaagc
     1861 tgatgatttt cgggaatgta aaaatagcct tgttgaataa agtcggcatt gttaggagat
     1921 gttatgattg cgatcgttct catataactt gtaggaacag ctcctgtgcc tgtccgatta
     1981 taatggagta ctggagggca caggaccctc ccctacaaga ctatattttt tctgaaagat
     2041 acggatcttc ataccctaaa ctttgcataa tttcggtttc gaggctttcc atttccactg
     2101 cttcatcttc ctcaatatgg tcgtagccga gcagatgaag tgtgccgtga atcgccaaat
     2161 gagcccaatg ggattcggct gaaatacctt gttcttccgc ctctttttcc attacttgac
     2221 gacagatgac taaatcaccc aatagtggca tttcgattcc ttcgggggcc tcaaacggga
     2281 aagatagcac attggttggc ttgtctttac cacgataagt cagatttagc tcgtggcttt
     2341 ctgcttcatc gacaatccga atggtgatct cactctcagg gaaatcatcg gtttttgcct
     2401 caactgcgag ggctttttgt acccaagtat aaaattgttg ttcacttggt aagttttcgg
     2461 tattttcgca ggcgatctgc aaatcaatat ataaattttc cattcgttac cttttgcaaa
     2521 ttattgccat tttttctatt gccatttttt ggcggcttgt tggtcactct cacgcccttc
     2581 aatccaacgt tcaccggctt gggtttgttc tcgtttccaa aatggggctt tggttttgag
     2641 ataatccata ataaactcgt tggcgtggta ggcatcacct cgatgagcag aactcacacc
     2701 gactaatacg atttcatcac cggtatgcag ttgcccaatg cggtgaatca ccgcaacgcg
     2761 ttgtaaatcc caacgagtgc gagcttgagc cacaatttct tgcaatgctt ttttagtcat
     2821 tgccggatag tgttcaagaa acaaaccgga aacattatcg cccaaattca tttcacgtac
     2881 tttaccgatg aatagagtgg ctgcccccac gctatgttgt tcactgagcc attggtaaat
     2941 gcggttttgg tcaaattcag cttcttgtac ttcaatcagg gtttcaaaca ttttagcccc
     3001 cggttaccgg tgggaagaat gccacttcat cgccgtcttt gatctccgtt tcaagcgggc
     3061 agatcgtttg attaattgca accagcagtt tgcctttttc aagggcaagt tgccattttt
     3121 taccctgttg gcttaagtgt tcacgcaacg cttcggcagt ggtaaaagta ggctcaacat
     3181 ctagtctatc cgttgccaca agttcacgca cttgggcgaa aaaaagtatc ttaatcaacc
     3241 ttgaaatctc ctgattttcc gccactttta ctaagtaatc ttacattttc aataatcata
     3301 tctttttgca cggctttaca catatcgtaa atagtcagag cagcaacact agctgcggtt
     3361 aatgcctcca tttccacgcc ggttttgccg gttagtttac agagtgattc aattcgcact
     3421 tggttgcttt ccggcaacgc ctccagttga acttccactt tagaaagcaa taacgggtgg
     3481 cagagtggaa tcagctccca agttctcttc gccgcctgaa taccggcaat tcgagcggtt
     3541 gcgaatacat cgcctttgtg gtgattacca ctgacgatca tttgtaatgt ttcggcattc
     3601 attcgcacaa atgcctccgc tcgggcaatg cgtacggttt cttgtttcat tgaaacatca
     3661 accatattgg cttcgccgtt ttggttgatg tgggtgaagg ttgtcatttg tttgtctctt
     3721 taaatttgat tgtaaaatta atattgagcg tcttcttgtt actttctttg cttg
//
LOCUS       DANLXF010000219         3764 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.219, whole genome shotgun sequence.
ACCESSION   DANLXF010000219 DANLXF010000000
VERSION     DANLXF010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.219"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(184..1686)
                     /gene="ppx"
                     /locus_tag="PXD66_002186"
     CDS             complement(184..1686)
                     /gene="ppx"
                     /locus_tag="PXD66_002186"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HDL6320437.1"
                     /translation="MSSNIAPREFAAIDLGSNSFHMIVARIVNGSIQVLSRLKRRVRL
                     ADGLDENLVLSQDAIDRGIACLALFAKRLEGFSAENVKVVGTYTLRRAINNQAFLEQA
                     KAVFPYPISIISGQQEARLIYSGVSHTQPETGRKLVVDIGGGSTEMTIGDDFTPIRAE
                     SRHMGCVSFAKRFFPQGELTIERFDSAYQLAMEKIEDLMWEYKELGWDYALGSSGTIK
                     TVHQVLIANGYRDGLITESRLQKIIDICLEFKSLNEIKLRGLLDERADVLVPGLAILL
                     ALFHTFRIDVLRYSNGALREGVMYGLEKSFQVGDIRQRTAEALAEQFALDKQQAKRVE
                     HTALILFDQIKTWKNRRQVQDLRSMLKWASLLHEIGIVVNHNNVHRHSAYLIANRDLP
                     GFDFEQQTLLSVLMRYHLKGFKRSDIRSINRYQCRDVLTLVRVFRLAVLLNRSRQATV
                     VPKQLNLVIENNAWQLTFEQGFLANNPLISIDLEEEQKILASTNLQFAVS"
     gene            1882..2211
                     /locus_tag="PXD66_002187"
     CDS             1882..2211
                     /locus_tag="PXD66_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HDL6320438.1"
                     /translation="MSEVNKSFHDMLEYVHLYRLKNKLHRETADNDRKIRDNQKRVLL
                     LDNLTQYITDEMSIEEVRAIISNMRDDYENRVDDYMIRNAELSKQRREIRQKMASHKQ
                     APTNKKA"
     gene            complement(2275..3705)
                     /locus_tag="PXD66_002188"
     CDS             complement(2275..3705)
                     /locus_tag="PXD66_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIBC"
                     /protein_id="HDL6320439.1"
                     /translation="MSTLTYLQKIGRALMVPVAALPAAALLMGIGYWIDPDGWGTNSQ
                     LAALLIKSGAAIIDNMSILFAVGVAFGMSKEKDGAAALSGLVGYLVITTLLSPGTVAQ
                     LKGIAVDQVPAAFGKINNQFIGILVGVISAEIYNRFYQVELPKALSFFSGKRLVPIVV
                     SFILLYVWPVIFGALVSFGESIKDMGALGAGIYAFFNRLLIPVGLHHALNSVFWFDVA
                     GINDIPNFLGGAKSLAEGKAVIGVTGMYQAGFFPMMMFGLPAAALAIYHSAKPAKKMQ
                     VASIMFAAALASFFTGITEPLEFAFMFVAPVLYVVHALLTGLSVYIAASMEWIAGFGF
                     SAGLVDLVLSTRNPLAVQWYMLLAQGIVFAVIYYVLFRVVIQAFNLKTPGREEDSEVQ
                     TVNTVSRSEQAAKFVEALGGKENITSIDACITRLRLTLNDTAKADETKLKALGSKGNI
                     KLGENGYQVILGPEAEFVADAMKKLN"
BASE COUNT         1232 a          857 c          652 g         1023 t
ORIGIN      
        1 acactccaat agaagtagga taattcgtaa aatatataat ctcaataagc aaacatcaaa
       61 aatgagaagt tgatcaaatt ttaaaaaata ctttttgcaa aaaattagct aaatttgacc
      121 gcttgttcgc aaaagggaat gcaataaacg aaaaaggaag ccttcgcttc cttcatttaa
      181 atgttaagag accgcaaact gaagatttgt actcgccaat attttttgct cttcttctaa
      241 atctattgaa atcagtggat tattcgctaa gaagccctgt tcaaaggtaa gttgccaagc
      301 attattttca atcaccaaat tcaactgttt cggcactaca gtcgcttggc gagaacggtt
      361 taatagcacc gctagccgga aaacccgtac caacgttaat acatctctac attggtaacg
      421 gttgatacta cgaatatccg agcgtttgaa tcctttaagg tggtatcgca ttaataccga
      481 tagaagggtt tgctgttcaa aatcaaatcc cggtaaatct cggtttgcaa tcaaataagc
      541 agaatgacga tgcacattat tgtggtttac aacaatacca atctcgtgta ataaacttgc
      601 ccatttcagc attgaacgca gatcttgcac ttggcggcgg ttcttccaag ttttgatttg
      661 gtcaaataaa atcaacgccg tatgctccac cctttttgct tgctgtttat ctaaagcaaa
      721 ttgctcagct aacgcttctg cagtccgttg gcgaatatcc ccaacttgga aacttttctc
      781 caaaccatac attacccctt ctcttaaagc cccgttggaa taacgtaata catcaattct
      841 aaaagtgtga aaaagtgcta aaagaatagc taagcccgga actagcacat ctgctcgttc
      901 atcaagtaag cctcgtagct taatctcatt taacgattta aactctaggc aaatatcaat
      961 aattttctgt aggcggcttt ctgtaattaa gccatcacga tagccattgg caatcagcac
     1021 ttgatggacg gttttaatcg taccggaaga acctaaagca tagtcccaac ctaactcttt
     1081 gtactcccac attaaatctt cgattttttc cattgcaagc tgataggcac tatcaaaacg
     1141 ctcaatcgtt aattccccct gcgggaaaaa gcgttttgca aaactcacac aacccatatg
     1201 acgactttcc gctcgaatcg gggtaaagtc atcaccaata gtcatttccg ttgaaccacc
     1261 gccaatatct accactaatt ttctgccagt ttcgggttgg gtatggctaa cgcccgaata
     1321 aatcagcctc gcctcttgtt gtccggaaat aatggaaatc gggtaaggaa atactgcttt
     1381 cgcttgctcc agaaaagctt ggttattgat tgctctacgt aaggtataag tgccaaccac
     1441 cttcacattc tctgcagaga acccttctaa acgttttgca aataaagcta aacaagcaat
     1501 gcctctatca atcgcatctt ggcttaatac taagttttca tcaagcccat ctgccagccg
     1561 tacacgccgt ttcaaacggg aaagcacttg aatggaacca tttacgatac gggctacaat
     1621 catatgaaag ctgttcgagc ctaaatcaat agctgcaaac tctctaggag caatatttga
     1681 tgacataatg aaaccctgta ccaataactg aaataagtaa gatattacca tttttgagcc
     1741 ctagatatca ctacaaattt agcgttattc gcgagaatta atttaaatca taaaatgtga
     1801 taaatttttt tgacgaattg acggaaaaag aataggattt acgagcttac ttaattcaac
     1861 actacatcaa gggagattga tatgtctgaa gtgaataaat cattccacga tatgctggaa
     1921 tatgtgcatc tttatcgtct aaaaaacaaa ctgcaccgtg aaacggcgga taatgatcgc
     1981 aaaattcgag ataatcagaa gcgtgtactt ttattagata acctaacgca atatattacc
     2041 gacgaaatgt ctatcgagga agttcgagcg attatttcca atatgcgtga tgactatgaa
     2101 aatcgagtag atgattatat gatccgtaat gcggaacttt ccaaacaacg ccgtgaaatc
     2161 cgccaaaaaa tggcctctca caaacaagca ccaaccaata aaaaagcata aaaagccaag
     2221 cggtctgatt tgcaaaaaaa tatgcaaatc agaccgcttg tgattaagca gagattaatt
     2281 cagctttttc attgcatctg ccacaaattc tgcttccggt cccaaaatca cttggtagcc
     2341 attctcgcct aatttgatat taccttttga ccctaacgct ttcaatttgg tttcgtctgc
     2401 tttcgccgta tcatttaagg ttaggcgtaa gcgggtaata caagcatcaa tgctggtaat
     2461 attctcttta ccacctaatg cttcaacaaa tttggcagct tgttcgctac gagaaacagt
     2521 attcactgtt tgtacttcgc tatcttcctc acgccccggc gtttttaaat taaaggcttg
     2581 gatcactact cggaacaata catagtaaat tactgcgaat acgatacctt gggccaataa
     2641 catataccac tgcactgcca gcggatttcg tgtcgataac actaaatcga ccaaccctgc
     2701 actaaaaccg aaaccggcaa tccattccat tgaggctgca atataaacag ataatccggt
     2761 taataatgcg tgaaccacat aaagcacagg tgcaacaaac ataaaggcaa attcaagcgg
     2821 ttccgtaatg ccggtgaaga acgaagctaa agcagcagca aacataatag aggctacttg
     2881 cattttctta gccggtttcg cactgtggta aatagctaat gctgccgcag gtaagccaaa
     2941 catcatcatc gggaagaaac cagcttgata catacctgtt actcctatca ctgctttgcc
     3001 ctcagccaat gattttgccc cacctaagaa gtttggaata tcattaatgc ctgcaacatc
     3061 aaaccagaaa accgagttta atgcgtggtg taatccaacc ggaattaata aacgattaaa
     3121 gaaagcataa atacctgccc ctaacgcccc catatctttg atagattccc caaaagagac
     3181 taaagcacca aaaataaccg gccagacata aagcaagata aatgacacca caatcggcac
     3241 caatcgttta ccgctaaaga aagacaatgc tttcggtaac tctacttggt agaaacggtt
     3301 ataaatttcg gctgaaataa cccctactaa gatcccgata aattggttgt tgattttacc
     3361 gaatgctgca ggcacttgat caactgcgat cccttttagc tgagcaacgg tccccggaga
     3421 caacaaagtg gtaattacta aatacccgac taaaccggat aatgctgctg ccccatcttt
     3481 ttcttttgac ataccaaatg ccacacccac ggcaaataga atagacatat tatcaataat
     3541 tgccgcccca gatttaatca gcaatgccgc taattggcta tttgttcccc agccgtccgg
     3601 atcaatccaa tagcctatac ccatcaataa tgctgctgct ggtaatgcag caaccggcac
     3661 cattaatgca cgcccaattt tttgtaaata ggttaatgta ctcataaaag cctccaaaaa
     3721 tacctcataa tgagtaactt aagcatagaa aaaaaccgat taaa
//
LOCUS       DANLXF010000220         3740 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.220, whole genome shotgun sequence.
ACCESSION   DANLXF010000220 DANLXF010000000
VERSION     DANLXF010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.220"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..913)
                     /locus_tag="PXD66_002189"
     CDS             complement(<1..913)
                     /locus_tag="PXD66_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HDL6320440.1"
                     /translation="MKLTKIVLSVVAVIGAVAVGGSWYTGKQVEEKYQELVVQANNQL
                     KQLSGEFGANIELKDVQIDRHFFISNAKYRLEVDLGNGKKLDFVGNDKLHHGPLPLNR
                     LAKLNLAPVMMSAESKLQAPEALKKLMGEHIGTGFSNISYSGDVEGHFDISPIKHSDE
                     TGHIETTPIKVQYSYDQKLKGVDSTVELESLKIKAEEFDFQIQGIVYDVQTENNESYP
                     NLTLGKGSGKINAIEFKSKDGKLSQIKDVVVKGDNLLKGDRLISTGDLEAATFNIEGV
                     EMGKLKMDMAYDLDAKLTNDITPLLSNP"
     gene            complement(963..1769)
                     /gene="trpA"
                     /locus_tag="PXD66_002190"
     CDS             complement(963..1769)
                     /gene="trpA"
                     /locus_tag="PXD66_002190"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HDL6320441.1"
                     /translation="MSRFDTLFTALKAKNEGAFVPFVTLCDPGFERSFEIIDTMIKNG
                     ADALELGFPFSDPLLDGPVIQAANKRALDGGYSTDSCFELIAKIRAKYPDIPIGLLLC
                     ANLIFVPTAEVFFQRCQQSGVDAVLIADLPVFAAQEFVPTAEKYGIQPVFICPPNADQ
                     NTIERIAQLTQGYTYLVSRAGVTSAENQAHASNLDNLVESLKQAGAAPILQGFGIAKP
                     EQVKEALRLGADGAISGSAIVKIIERNLADQANLLKELAEFVQVMKAATK"
     gene            complement(1771..2961)
                     /gene="trpB"
                     /locus_tag="PXD66_002191"
     CDS             complement(1771..2961)
                     /gene="trpB"
                     /locus_tag="PXD66_002191"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HDL6320442.1"
                     /translation="MSDTLLNPYFGEFGGMYVPEILVPVLKELEQAFVDAQQDPTFQA
                     EFADLLKNYAGRPTALTLCRNLTKGSKTKLYLKREDLLHGGAHKTNQVLGQILLAKRM
                     GKTRIIAETGAGQHGVATALACAMLGMPCVVYMGAKDVERQSPNVFRMRLMGAEVIPV
                     QKGSCSLKDACCEAMRDWAANYDTTHYLIGTAAGPHPFPTMVREFQKMIGEEIKRQIL
                     AKEDRLPDAVIAAVGGGSNAIGMFADFIEEQSVKLIGVEPAGKGIETGEHGAPLKHGT
                     TGIYFGMKSPIMQDKDGQIEESYSISAGLDFPSVGPQHAYLNATGRAEYVSITDAEAL
                     EAFQALAKNEGIIPALESSHALAYALKMIKQNPEKEQLLVVNLSGRGDKDIFTVDKIL
                     NGGE"
     gene            complement(2973..3725)
                     /locus_tag="PXD66_002192"
     CDS             complement(2973..3725)
                     /locus_tag="PXD66_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL6320443.1"
                     /translation="MKIALVTGVTAGFGLAICKLLLQSGYKVIGTGRRAERLEELHLQ
                     FGDKFLPLAFDIQQPDEVEKALNSRPKAWQQIDLLVNNAGLALGLEPAQKADLNDWYQ
                     MIDTNIKGLVTMTRLVLPEMVERKSGHIINIGSIAGTYPYPGGNVYGGTKAFVKQFSL
                     NLRADLAGTKVRVSNVEPGLCGGTEFSNVRFKGDDEKAAKVYENVQFVRPEDVANIVL
                     WLNQQPEHVNINRVEVMPVAQTSSALNVARNI"
BASE COUNT         1032 a          893 c          675 g         1140 t
ORIGIN      
        1 gaggattaga taataaaggc gtaatatcat tggttaattt agcatctaaa tcgtatgcca
       61 tatccatttt taatttaccc atttcaacac cctcaatgtt gaaagtggct gcctctaaat
      121 ctccggtaga aattaaacga tcgcctttta atagattatc gcccttcacc acaacatctt
      181 taatttgaga taatttgcca tctttagact taaactcaat agcgttaatt tttccactac
      241 ctttgcctaa agttaaatta ggataacttt cattattttc tgtttgaaca tcataaacta
      301 taccttgaat ttgaaaatca aattcttctg cttttatctt aagactttct agctcaacag
      361 tgctatcaac cccttttaat ttttggtcgt agctatattg cactttgatt ggcgtggttt
      421 caatatgacc tgtttcatca ctatgtttaa tcggggaaat atcaaaatgc ccttccacat
      481 cgcccgaata gctgatatta gaaaaaccgg tgccaatatg ttcacccatc aattttttaa
      541 gggcttcagg agcttgtaat ttgctttccg cactcatcat tacaggagct aaattaagtt
      601 tcgctaaacg attaagcggt agcggaccgt gatgaagttt atcattacca acaaagtcca
      661 atttcttacc attacccaaa tccacttcta aacgatattt cgcattagag ataaagaaat
      721 ggcgatcgat ctgcacatct ttaagctcaa tattcgcacc aaattcgcca ctcagttgtt
      781 taagttggtt attagcttga acaaccaatt cttgatattt ttcttcaacc tgcttacctg
      841 tgtaccaact tccgccaact gcaacagcac caattaccgc tacaactgaa agaacaattt
      901 ttgttaattt cattctgttt ccttttattt tcaaaaaaat ttaaaaaatg accgcttgta
      961 gtttacttcg tagccgcttt catcacttgt acaaattcgg caagttcttt gagcaaatta
     1021 gcttggtcag ctaaattacg ttcaataatt ttcacaatcg ctgagcctga aatcgccccg
     1081 tctgccccaa gccttaacgc ctcttttacc tgttcaggtt tggctatgcc aaaaccttgt
     1141 aagatagggg cagctccggc ttgttttaaa ctttccacta aattatccaa attacttgcg
     1201 tgagcttggt tttcagcact agttacaccg gcacgggaaa ctaaataagt gtagccttgg
     1261 gttaattgag caatccgttc aatggtgttt tgatcagcat tcggcgggca aataaacacc
     1321 ggctgaattc catatttttc agcggttggc acaaactctt gagcagcgaa aacaggcaaa
     1381 tcagcaatca acaccgcatc aactccactt tgttggcaac gttggaaaaa tacttctgcg
     1441 gttggcacaa aaattaaatt ggcacacagt agtaaaccga taggaatgtc tggatattta
     1501 gctcgaattt tggcaatcag ctcaaaacag ctatcagtgc tatagccacc atctaaagct
     1561 cgtttattcg ctgcttgaat caccggcccg tctaagaggg ggtcagaaaa cggaaagcct
     1621 agctctaaag catcagcccc gtttttaatc atcgtgtcga taatctcaaa actgcgttca
     1681 aaaccaggat cgcataatgt cacaaacggc acaaaagccc cttcgttttt tgcttttaat
     1741 gcagtaaata gcgtatcaaa gcggctcatt ttactctcct ccatttaaaa ttttatccac
     1801 ggtgaaaatg tctttatcgc ctcgacccga taaattcacc actaataact gctctttttc
     1861 agggttctgc ttaatcattt ttaacgcata ggctaaggcg tgggaagatt ctaacgccgg
     1921 aataatcccc tcatttttgg ctaatgcttg gaatgcctct aaagcctctg catcggtaat
     1981 cgatacatat tcagctcgcc ctgtcgcatt taaataagcg tgttgcggcc cgacagaagg
     2041 gaaatctaag ccggctgaaa tggagtaaga ctcttcaatt tgcccgtctt tatcctgcat
     2101 aatcggcgat ttcataccga aatagatccc cgttgtgccg tgtttgagcg gtgcaccgtg
     2161 ttcaccggtt tcaattcctt ttcctgccgg ctcaacaccg atcaatttga cggattgctc
     2221 ttcgataaaa tctgcaaaca taccgatagc atttgagcca ccgcctacgg cagcaatcac
     2281 cgcatcaggc aagcggtctt cttttgctaa aatttgacgt ttgatctctt cgccaatcat
     2341 tttctgaaat tcacgcacca ttgtcgggaa cgggtgcggg ccggctgcag tgccgattag
     2401 gtaatgggta gtatcataat ttgccgccca atcccgcatt gcctcgcaac aggcatcttt
     2461 gagcgagcaa gagccttttt gtaccggaat cacctccgct cccattaaac gcatacggaa
     2521 tacatttggc gattggcgtt ctacatcttt tgcgcccata tacaccacgc aaggcattcc
     2581 taacatcgca cacgctaatg cggttgccac accgtgctgt cctgctcctg tttcggcaat
     2641 aatgcgggtt ttacccattc gtttagcgag taagatttgc cctagcactt ggttggtttt
     2701 atgtgcccca ccgtgtaaaa gatcttcacg ttttaaatag agtttggttt tactgccttt
     2761 ggttaaatta cgacaaaggg ttaaagcagt cgggcgaccg gcatagtttt tgagtaaatc
     2821 ggcaaattca gcttggaatg ttgggtcttg ctgagcatct acaaaggctt gttctaattc
     2881 ttttagcacc ggcactaaaa tttccggcac atacatacca ccaaattcac caaaataagg
     2941 attaagaagt gtgtctgaca taatatttcc ttttaaatat ttctcgctac atttaaggca
     3001 gaagaagttt gagccaccgg catcacttca acccgattaa tattaacgtg ttcaggttgc
     3061 tgattcagcc aaagcacaat atttgccaca tcttccgggc gcacaaactg cacgttttca
     3121 taaactttag ccgctttttc atcatcgcct ttaaagcgta catttgaaaa ttccgtaccg
     3181 ccacatagcc ccggctcaac attggaaaca cgcactttag tgccggctaa atctgcccgc
     3241 aaatttaggc taaactgttt cacgaaggct ttagtaccac catatacatt accgcccggg
     3301 taagggtaag tgccggcaat cgagccgata ttaataatat gcccactttt ccgctccacc
     3361 atttcaggca aaactaagcg tgtcatcgtt accaatcctt taatattggt atcgatcatt
     3421 tgataccaat cgttcaaatc tgctttctgt gccggttcta agccgagtgc aaggcctgca
     3481 ttgtttacta ataaatcaat ttgttgccac gctttagggc ggctattcag tgctttttcc
     3541 acctcatcgg gctgttgaat atcaaatgca agcggtagaa atttatcgcc aaattgtaaa
     3601 tgcagttctt ctaaacgctc tgcccgtctg cccgtgccaa ttactttata acccgattga
     3661 agcaataatt tacaaattgc caacccaaat ccagcagtta ctcctgtgac taatgctatt
     3721 ttcatctttt caccctcaaa
//
LOCUS       DANLXF010000221         3730 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.221, whole genome shotgun sequence.
ACCESSION   DANLXF010000221 DANLXF010000000
VERSION     DANLXF010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.221"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..1201
                     /locus_tag="PXD66_002193"
     CDS             <1..1201
                     /locus_tag="PXD66_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL6320444.1"
                     /translation="NAKSKDKEYILSDGDGLNLRVKPLPKGSKIWIFNYTNPQTKNRT
                     NLTIGRYPLITLAEARTICDGYRSLIEKGIDPQEEKKRLQQEAENRVNDTFRNVAESY
                     FNGIYKEKAKNPITREKNWIRLENHIFPYIGDKHVEEIKVKALVDIYSKVADKSNTLK
                     KLHQLVSAVMDHAITQGIIESHNCRLAVKNFYIKPSTPNPTIELEELPQLFTDLHNAK
                     LSKQTYALVCWSFLTALRPNEAVNAEWKEIDFEKKLWNIPKEKMKGKADKKRPHSVPL
                     SSQALKILEMMKMHSNSSPFVFPGRSSNSKPMSNATVNSTLKRNGYKDRLTAHGIRAF
                     VKTFLSSRKVDRHVSESILSHLLEGGDDLENTYNRYDFLEERVPVMQLLGDYCEQCGM
                     NLELVRG"
     gene            complement(1360..3228)
                     /locus_tag="PXD66_002194"
     CDS             complement(1360..3228)
                     /locus_tag="PXD66_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL6320445.1"
                     /translation="MFKKLLELRQQKAAIEAEMRSILTKADEEKRSTTEAEGAKFDEL
                     RSQAENLVKEIARYEAITDEQRNQPGQQVETANVKVSDDELRHWIKTGELRSLATNTN
                     GGADGGYSVIPELDKQVMKRLTDDSVMRQIANVVKLTQGKEYKKLVSAGGAAVNHIGE
                     GEARTETATPKMNEVTIAVHNIYAYPKTTQEILDFGGVDILGWLTEEISESFVETEET
                     DLTNGTGTKQAKGFLAYPRTTANDKTRPFGTLQKMEVQPATVIGWNSKGQPETLTPYP
                     PYSVNVQRTVNRQNWLTAYIPHSYDENVAETPHFDQWLNFVSDGKQDKAKNILAALYA
                     ILTNRYNWQVFFQVTGKGGSGKSVFAGIATLLAGEKNTASARLENFDDERKIAGLEDK
                     KLIICSEQTKYAGDGGGLKAITGGDMVRVDPKNKHPFNARIAAMIMIVNNDPCKWTER
                     NGGIDRRIVNFRFNKRPPENERDPYFMDKITLEIGGIIRKVLDTFPDPLEAKRALEEQ
                     KESLEALEIKKQSDPLTDFFEYLYTTENTDGLYIGTGNTLGHDKIRTHLYPAYLAFVK
                     AKNVLELGLNTFVVGIEQAVKQHGNKHDFTKRKTNKGQRTNVHFKNFDDFQSDILN"
     gene            complement(3239..3634)
                     /locus_tag="PXD66_002195"
     CDS             complement(3239..3634)
                     /locus_tag="PXD66_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6320446.1"
                     /translation="MISNEAIRQEVIDLLQGNIQGIQHFHNGRAVFTDVENELPAIAV
                     FIDEAEYSQLTLCDGECNAYLKIGIYLPMFSTENDLDLVAQKISDVMNQATFHSMDEC
                     VLAKYSYDYDANESAWKTATLHYNINYLN"
BASE COUNT         1050 a          763 c          734 g         1183 t
ORIGIN      
        1 aaatgccaaa tcaaaggata aagaatatat tttatccgat ggcgatggat taaatcttag
       61 agttaaacct ctccctaaag gctctaaaat ttggattttt aactacacta acccacaaac
      121 taaaaacaga acaaatttaa ctattgggcg ttacccctta attacacttg cggaagctcg
      181 cactatatgt gatggctacc gctcgttaat agagaaaggc atagatccac aggaagaaaa
      241 aaagcggctg caacaagaag cagaaaatcg agtaaatgat actttccgga atgtggccga
      301 aagctatttc aatggtattt ataaagagaa agcaaaaaat ccgattactc gtgaaaagaa
      361 ttggataagg ttagaaaatc atattttccc ctatatcggg gataaacacg ttgaagaaat
      421 caaagtaaaa gcccttgttg atatttattc taaagtggca gataaaagca acacattaaa
      481 aaaactgcat cagcttgtat ctgctgtaat ggatcacgcc attacgcaag gcattataga
      541 aagccataac tgccgattag cagttaaaaa cttttatatc aagccatcta cgccaaatcc
      601 cactattgag cttgaagaat taccgcagtt atttactgat ttacataatg cgaagttatc
      661 aaagcaaact tacgcccttg tttgttggtc gttcttgact gctctacgcc caaatgaagc
      721 cgttaatgct gaatggaaag aaatagactt tgagaaaaag ctatggaata tccctaaaga
      781 gaagatgaaa ggaaaagcag ataaaaaacg ccctcactct gtgccgttat cctcccaagc
      841 gttaaagata ttagagatga tgaaaatgca ctctaattct agcccttttg tttttccagg
      901 tcgttcatca aatagtaaac caatgagcaa cgcaacggta aattcaacat taaaaaggaa
      961 tggatataaa gatagactaa ccgcacacgg tattcgggca tttgtgaaaa catttctatc
     1021 ttctcgcaaa gtagatcgcc acgtttccga aagtatatta tcccatttat tagaaggtgg
     1081 agatgattta gaaaacacat acaaccgata tgatttttta gaagagcgtg tgccagtaat
     1141 gcagctttta ggtgattatt gcgaacagtg cggaatgaat ttagagttag ttcggggtta
     1201 gggaggtaaa atatatgatt ttagtcgtca cgtcatcact tttcccactt tcccctttaa
     1261 aatcaaatag ttaagtggtg atgactggtt ttaaagtcat cattaagtca tcacaaaaag
     1321 gtgatgacct agaaaaaaag aggggtttcc ccctctttgc taattcaaaa tatcactttg
     1381 aaagtcatca aaatttttga aatgaacgtt tgttcgttgc cctttattcg tctttcgttt
     1441 tgtgaaatcg tgcttattgc cgtgttgctt tactgcttgc tcaataccta ctacaaaagt
     1501 atttaagcct agttctagca cattcttagc cttaacaaag gctaaatatg ccggataaag
     1561 gtgcgttctt attttgtcgt gtccaagcgt gttgccagtt ccgatataaa gcccgtctgt
     1621 attttctgtt gtatatagat actcgaaaaa atccgttaat gggtcagact gctttttaat
     1681 ctccaacgcc tctaggctct ctttttgctc ctctaaggct cttttagctt cgagtgggtc
     1741 agggaaggta tcaagcactt tacggataat gccccctatc tctaatgtaa ttttatccat
     1801 aaaatacgga tcacgttcat tttcaggcgg tcttttatta aaacggaaat tgacaatccg
     1861 cctatctata ccgccattcc gttctgtcca tttacaaggg tcgttattga cgatcataat
     1921 cattgccgca attcttgcgt taaatgggtg cttgttcttc ggatcgactc gcaccatatc
     1981 accgccagta atcgctttca atccgccacc atctccagcg tatttcgttt gctcggaaca
     2041 gataatcagc tttttatctt ccagccctgc gattttgcgt tcatcatcaa agttttctag
     2101 ccttgctgaa gctgtgttct tttctccagc taatagtgtc gcaatgccag caaataccga
     2161 tttaccgctt ccgcctttcc ctgttacttg gaagaatact tgccagttgt agcggtttgt
     2221 taaaatcgca tagagagcgg ctagaatgtt cttcgccttg tcttgcttgc cgtctgatac
     2281 aaagttaagc cattgatcaa agtgtggtgt ttcggctacg ttctcgtcat agctatgtgg
     2341 aatataagcc gttagccagt tttggcggtt aacggttctt tgaacattta ccgaataagg
     2401 tgggtaaggg gttaaagttt cgggttgccc cttgctattc caaccaatca ccgtagccgg
     2461 ttgtacttcc attttttgaa gcgtgccgaa tggtcgggtt ttatcgttgg ctgtcgtgcg
     2521 tggataagct aagaagcctt tagcttgttt tgtgcctgta ccgttggtta aatcggtttc
     2581 ttcagtttcg acaaaggatt ctgaaatttc ttcagtcagc cagcctagaa tatctacacc
     2641 accaaagtct aaaatctctt gcgtggtttt cgggtaagcg taaatattat gaacggcaat
     2701 cgttacttca ttcattttcg gggtggcggt ttcggtgcgt gcttcgcctt caccgatatg
     2761 atttaccgct gcaccgccag ccgatactaa ttttttatat tctttgcctt gggtcagttt
     2821 caccacgtta gcaatttgtc gcataacaga atcatctgtt aggcgtttca ttacttgctt
     2881 gtcaagttcc ggaatcactg aatagccacc gtctgcccct ccatttgtat tcgtagcaag
     2941 cgaacgcaat tcgcctgttt taatccagtg gcgaagctcg tcatcagata ctttcacatt
     3001 tgcggtttca acctgttgcc ccggttggtt acgctgttca tcagtaatcg cttcataacg
     3061 tgcgatttct tttactaaat tttcagcttg tgagcggagt tcatcaaact tagccccttc
     3121 tgcttcagtt gttgagcgtt tttcttcgtc tgctttggtt aaaattgaac gcatttctgc
     3181 ttcaatagcc gctttttgtt ggcggagttc taaaagtttt ttaaacattg ccttatcctt
     3241 aattcaaata attgatattg tagtgaagcg ttgctgtttt ccacgctgat tcgttagcat
     3301 cataatcata gctatactta gccagcacac attcgtccat actgtggaat gtggcttgat
     3361 tcatcacatc actgattttt tgagccacta aatctaggtc gttttcggtt gaaaacattg
     3421 gtaagtaaat gccgattttc aaataggcgt tgcattcgcc atcgcaaagc gtgagttggc
     3481 tatattccgc ttcatcaatg aatacggcga tagccggtaa ttcattttct acatcggtga
     3541 atacggctcg gccgttatgg aaatgttgaa tgccttgaat gttaccttgt aataagtcga
     3601 taacttcttg gcggattgct tcgttagaaa tcataaaaaa gtcctcttaa ttaagaaggc
     3661 tacaattagc cacagaacaa tataggcata atttcaacgg agtaaatagg gtaaaattta
     3721 atcgtttagc
//
LOCUS       DANLXF010000222         3723 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.222, whole genome shotgun sequence.
ACCESSION   DANLXF010000222 DANLXF010000000
VERSION     DANLXF010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.222"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            77..1504
                     /gene="aspA"
                     /locus_tag="PXD66_002196"
     CDS             77..1504
                     /gene="aspA"
                     /locus_tag="PXD66_002196"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HDL6320447.1"
                     /translation="MSDVRIEHDLLGEREVPNSAYWGIHTLRAIENFNISNVTISDVP
                     EFVRGMVMVKKATALANGELGAIPKKIAKAIVAACDEVLVKGRCLDQFPSDVYQGGAG
                     TSVNMNTNEVIANLALEILGEAKGKYDIVNPMDHVNASQSTNDAYPTGFRIAVYNSIT
                     KLINKITYLQTAFENKATEFSDVLKMGRTQLQDAVPMTLGQEFKAFAVLLDEEVRNLK
                     RTAELLLEVNLGATAIGTGLNTPEGYSTLAVKYLAEVTQLPCSLSENLIEATSDCGAY
                     VMVHGALKRTAVKLSKVCNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPEV
                     VNQVCFKVIGNDTTVTFAAEAGQLQLNVMEPVIGQAMFESIELLSNACINLRDKCIDG
                     ITANRDICQNYVFSSIGIVTYLNPFIGHHNGDIVGKICATTGKSVREVVLERGLLTEE
                     QLDDILSIENLMNPTYKAKRFTEEE"
     gene            complement(1579..3597)
                     /gene="rep"
                     /locus_tag="PXD66_002197"
     CDS             complement(1579..3597)
                     /gene="rep"
                     /locus_tag="PXD66_002197"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HDL6320448.1"
                     /translation="MKLNSQQQQAVEYTKGACLVLAGAGSGKTRVIINKIAHLIAHCG
                     YSPKQIAAVTFTNKAAREMRERVDHSIGKENSKGLTISTFHTLGFEILKREYKLLGFK
                     SGMTLFDEQDQIALLKHLLPENVTEDKDLLKALIATISNWKNDLLSPERVLTRVRDER
                     ERVFSHFYQLYQNQLKAYNALDFDDLIMLPVLLFRQFPEAKSRWQQKIRYLLVDEYQD
                     TNTSQYELIKLIVGNEPNFTVVGDDDQSIYSWRGAKPENMQRLREDFDLQVIKLEQNY
                     RSSQRILHCANILIDNNDHLFSKRLFSELGQGEKLQVIEAKNEEHEAERIVGELIAHR
                     FSKKTKYKDYAILYRGNHQSRLLEKLLMQNRIPYKISGGTSFFARAEIKDMMAYLRLV
                     VNQDDDAAFLRIVNTPKREIGAATLEKLGILANEKQVSLFEAIFDFELIQRLTPKPYQ
                     ALQDFARWIVEIADQADRSDPIEAVKDLLAKIQYEAYLYEMAANPKVAEMQSRNVQTL
                     FEWVQAMLEGDEIEPPMNLVQVVNRLTLRDMLERGEEDDEADQVQLMTLHASKGLEFP
                     HVFLIGMEEGILPHQTSLDEDNVEEERRLAYVGITRAQQTLTFSLCKERRQYGEIIKP
                     EPSRFLLELPQDDLEWESEKPRMTEQEKEAKSSANIANLMAMLKANKK"
BASE COUNT         1063 a          824 c          732 g         1104 t
ORIGIN      
        1 ttagatcgca ttatttgtct attaaaatcg ttattatcta ccaaaatcga ttgtcattta
       61 ttttaaggag atgaatatgt cagatgtgcg tattgaacac gatttacttg gtgaaagaga
      121 agtacctaat agtgcttatt ggggcattca tacattaaga gcgattgaga attttaatat
      181 ctcaaatgtc accatttctg atgtacctga atttgtgcgt ggcatggtaa tggtaaaaaa
      241 agctactgcg ttggcaaatg gtgaattggg tgcaatcccg aaaaaaattg ccaaagctat
      301 tgtggcagcc tgtgatgaag tattagtgaa aggacgctgt ctcgatcaat tcccttcaga
      361 tgtttatcaa ggtggagccg gcacatcggt gaatatgaat accaatgaag tgattgccaa
      421 ccttgcatta gaaattttgg gcgaggcaaa aggtaaatat gatattgtca atccaatgga
      481 tcacgttaat gcaagccaat ccaccaatga tgcctatcca accggtttcc gcatcgcggt
      541 ttataacagt atcactaaat taatcaataa aatcacttac ctacaaactg catttgaaaa
      601 taaagccact gagttttccg atgtactaaa aatgggaaga acgcagttac aagatgccgt
      661 gccaatgacg ctcggtcagg aatttaaagc ttttgccgta ttattagatg aagaggttcg
      721 taaccttaaa cggacggcag aattgcttct tgaagtgaat ttaggcgcaa ccgctatcgg
      781 tactggtctg aatacgccag agggttactc tactcttgca gttaaatatt tagccgaagt
      841 cacgcaactg ccttgctcac tgtctgaaaa tctcattgag gcaacatctg actgtggtgc
      901 ttatgtaatg gttcacggtg cattaaaacg caccgccgtc aaactttcta aagtgtgtaa
      961 tgacttacga ttactttcat caggcccacg tgccggctta aatgaaatta atcttccgga
     1021 attacaagcc ggctcatcta ttatgccggc aaaagtaaac cctgttgtac ctgaagtcgt
     1081 aaaccaagtg tgctttaaag tgattggtaa cgacaccacc gttacttttg cagcagaggc
     1141 cggtcaatta cagcttaacg taatggagcc ggttatcggg caagcaatgt ttgaatctat
     1201 cgagctgctt tccaatgcat gtattaatct acgtgataaa tgtattgacg gtattaccgc
     1261 taaccgtgac atctgccaaa actatgtgtt cagctctatt ggtattgtga cctacctcaa
     1321 tccatttatc ggccaccata atggtgatat tgtcggcaaa atttgtgcaa ccaccggcaa
     1381 aagtgtacga gaagttgtgc ttgagcgagg cttactcacc gaagaacaac tcgatgatat
     1441 tctctcgatt gagaacttaa tgaatccgac ttataaagcc aaacgcttta ccgaagagga
     1501 ataatcattg tttacaagcg gtctgattct ggcaaaaaat tgcaaatttt taccaaaatc
     1561 agaccgcttg ttatcccatt atttcttatt tgctttcagc atcgccatta aatttgcaat
     1621 atttgcagaa gattttgcct ctttttcctg ttcggtcatt cttggctttt cactttccca
     1681 ctccaaatca tcttgtggca attccagtaa aaaccggctc ggctcaggtt tgatgatttc
     1741 gccatattgg cgacgttctt tacacagtga aaacgtgagc gtttgttggg ctcgggtaat
     1801 gccgacataa gccaaacgcc gctcttcttc tacattgtcc tcatcaaggc tggtttggtg
     1861 tggcagaatc ccctcttcca taccgattaa gaaaacgtgc ggaaattcca accctttcga
     1921 ggcgtgaaga gtcattagtt gaacttgatc ggcttcatcg tcctcttcgc cacgttcgag
     1981 catatctcgc aaggttaaac ggtttaccac ttgcaccaaa ttcatcggag gctctatttc
     2041 atcgccctct aacatcgctt gcacccattc aaacaaggtc tgcacattac ggctttgcat
     2101 ttccgctact tttgggttcg ccgccatttc atacaaatag gcttcatact gaattttcgc
     2161 caacagatct tttactgctt caatcggatc ggatctatcg gcttgatcgg caatttccac
     2221 aatccagcgg gcaaaatcct gtaaggcttg gtaaggcttt ggcgtgaggc gttgtatgag
     2281 ttcaaaatca aaaatcgcct caaacaaact gacctgcttt tcattagcaa gtatgcctaa
     2341 tttttctagc gttgccgccc caatttcccg tttcggcgta ttcacaatac gcaagaaagc
     2401 tgcatcatcg tcttgattca ctaccaaccg cagatacgcc atcatatctt tgatttccgc
     2461 tcgagcaaaa aatgacgtac cacccgaaat tttatacgga atgcggtttt gcattagcaa
     2521 tttttccaac aagcgggatt gatggttgcc tcgatacaaa attgcataat ctttgtattt
     2581 ggtctttttc gagaaacgat gggcaatcag ctcgcccaca atgcgttccg cttcgtgttc
     2641 ctcatttttt gcctcaatga cctgtaattt ttccccttgc cccagttctg aaaacagccg
     2701 cttgctaaac aagtgatcgt tattatcaat cagaatattg gcacaatgca gaatccgctg
     2761 gctggaacgg taattttgct caagtttaat cacttgcaaa tcaaaatctt cccgtaaccg
     2821 ttgcatattt tcaggcttcg ccccacgcca agagtaaatt gattggtcat catcgcccac
     2881 gaccgtaaag tttggctcgt tgccgacaat cagcttaatt aactcatatt ggctggtgtt
     2941 ggtgtcttga tattcatcaa ctaacaaata gcgaattttc tgttgccaac gagattttgc
     3001 ttcgggaaat tggcggaaca gcaacaccgg caacataatc aaatcatcaa aatccaacgc
     3061 attataagcc ttaagttgat tctggtagag ctgataaaaa tgtgaaaaca ctcgctcacg
     3121 ttcatcacgc acacgagtaa gtaccctttc aggcgaaagc aaatcgtttt tccagttaga
     3181 aatagtggca attaaggctt tcagcagatc cttatcttcc gtcacatttt caggcaataa
     3241 atgtttcagc aaagcaattt gatcctgctc atcaaacaag gtcatacccg atttaaaacc
     3301 gagcaattta tattcgcgtt ttaaaatttc aaacccaagt gtgtgaaaag tcgaaatggt
     3361 taaacctttg ctgttttctt tgccgataga atgatccacc cgttcacgca tttcccgagc
     3421 agctttgttg gtaaaggtta ctgctgcaat ctgcttaggc gaatagccac aatgtgcaat
     3481 taaatgtgcg attttattaa taatcacccg agtcttaccc gagccggcac cagccagcac
     3541 aaggcaagcc cctttggtat attctactgc ttgttgttgt tgagaattga gtttcattga
     3601 atctattcaa aagtaatttt gacaactatt ttaacggaaa gaaaacaagc ggtcgaattt
     3661 tcaggaaaat ttgcaaaaaa ctttgaaaat ccgaccgctt gttcatcaga gaaaaatccc
     3721 caa
//
LOCUS       DANLXF010000223         3722 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.223, whole genome shotgun sequence.
ACCESSION   DANLXF010000223 DANLXF010000000
VERSION     DANLXF010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.223"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(293..1597)
                     /locus_tag="PXD66_002198"
     CDS             complement(293..1597)
                     /locus_tag="PXD66_002198"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HDL6320449.1"
                     /translation="MESIIALFKGMGLMHLELGQAIMIVISLVLLWLAIARKFEPLLL
                     LPIGFGGLMSNIPEAGMAMTALDNLLHSGSAEQLAIIAAKVNSTSLDLHSIKDAIALA
                     APSVQNELEVMAGDMGYTAGVLALFYKVAIGYGVAPLIIFMGVGAMTDFGPLLANPRM
                     LMLGAAAQFGIFATVLGALGLNWLGIIDFTLPQAAAIGIIGGADGPTAIYLASKLAPE
                     LLGAIAVAAYSYMALVPLIQPPIMKALTTEQERKIRMVQLRNVSNREKIIFPIVLLLL
                     VALLLPDAAPLLGMFCFGNLLRTSGVVERLNDTAQNALINIVTIFLGLSVGAKLVADK
                     FLQPQTLGILVLGVIAFGIGTASGVIMAKIMNRFSQHKINPLIGSAGVSAVPMAARVS
                     NKVGQEYDKQNFLLMHAMGPNVAGVIGSAIAAGVMLKYISTM"
     gene            complement(1607..3433)
                     /gene="oadA"
                     /locus_tag="PXD66_002199"
     CDS             complement(1607..3433)
                     /gene="oadA"
                     /locus_tag="PXD66_002199"
                     /EC_number="7.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-extruding oxaloacetate decarboxylase
                     subunit alpha"
                     /protein_id="HDL6320450.1"
                     /translation="MTTQPKKIAITDVVLRDAHQSLFATRLRLDDMLPIASELDNIGY
                     WSLETWGGATFDSCIRFLGEDPWVRLRELKKAMPKTPMQMLLRGQNLLGYRHYADDVV
                     DKFVERSVANGMSVFRVFDALNDPRNMQAALQAVKKHGGHAQGTLSYTTSPVHTLQTW
                     LDVTEQLLEIGVDSLVIKDMSGILTPMAAYELVSEIKKRYDVELHLHCHSTTGMAEMA
                     LLKAIEAGVDGVDTSISSMSGTYGHPATESIVATLQGTPYETGLSIPALEKISAYFRN
                     VRKKYAKFEGQLRGIDSRILVAQVPGGMLTNLESQLKQQNASDKLDLVLQEIPRVRED
                     LGNIPLVTPTSQIVGTQAVMNVLMGERYKTIAKETAGILKGEYGRTPAPVNKALQDRV
                     LEGAEPITDRPADHLPPELEKLVAEVTASAKEKGITLSENMIDDVLIVALFQQVGWKY
                     LENRNNPSAFEPVPSADEAKPAQASAASAPKAQTQSGSAIYTVELEGKAFVVKVSEGG
                     DITNIAPTPATPAPQAVDSAPKSANTLSANAEPVNAPMAGNILKVEVKEGQQVAEGDV
                     LLILEAMKMETQICAAKSGTIQGINVKQGDVVAVNDTLMSIA"
     gene            complement(3473..>3722)
                     /locus_tag="PXD66_002200"
     CDS             complement(3473..>3722)
                     /locus_tag="PXD66_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit gamma"
                     /protein_id="HDL6320451.1"
                     /translation="PAELFAEGINLMVAGMGFVMLFLLVLIYAISFISKIINQYFPEP
                     VSIPASKTVPQSPPDDLERLRPVIAAAIAHHRRINGQH"
BASE COUNT         1001 a          864 c          823 g         1034 t
ORIGIN      
        1 ataggcgaaa aaaaacgccg tattttgtgg gggaatacgg cgtaaagagt tggagtgatt
       61 acctattatt tttgaagtga tttaatgata atgattatca tctgttaagt caatactttt
      121 tttgaaaatt ttcaataaga attattctca tcaattaagt aagatggata ctacggcaat
      181 tagtgaagaa tgtacagcgg aattttcgta ttgtaagagt gttggtaagc ggtattttta
      241 gaaaaaacct ccgagagcgg ttatttttcg gaggtttttt gcaaatgagg atttacatcg
      301 ttgaaatgta tttcaacatc acccccgctg caatcgcaga gccaatcacg ccggcaacgt
      361 ttgggcccat tgcgtgcatt aacaagaagt tttgtttatc gtactcttgt ccgactttgt
      421 ttgatacccg tgcagccatc ggcacggcag aaacacccgc tgagccgata agtgggttga
      481 ttttgtgctg actaaagcgg ttcattattt tcgccataat cacaccgctt gcagtgccga
      541 tcccaaaggc aatcacgccc aatactaaaa tgcctagtgt ttgcggttgt aagaatttgt
      601 ctgcgaccaa ttttgccccc accgataacc ctaagaaaat cgtcacgata ttgattaacg
      661 cattttgggc ggtgtcgttt aagcgttcta ctacgccact ggttcgcagt aagttgccaa
      721 agcagaacat tcccaataac ggtgcagcat ccggcagtaa gagtgccact aataatagca
      781 acacgatagg gaaaataatt ttctcacggt tgcttacatt acggagctga accattcgga
      841 ttttacgttc ttgctcggtg gttaaggctt tcataatcgg cggctgaatc agtggcacta
      901 acgccatata agaataagcc gctaccgcaa tcgcacctaa taattcaggg gcaagtttgc
      961 tggcaagata aatcgcggtc gggccatctg ccccgccgat aatcccaatc gcagcggctt
     1021 gtggcaatgt gaagtcgata atgcctaacc agtttagccc taatgcaccc agtacggtag
     1081 caaaaatccc aaattgagcc gcagccccaa gcattaacat tcgagggttg gcaagtaaag
     1141 ggccgaaatc agtcatcgcc ccgacaccca taaagataat aagcggagca acgccgtagc
     1201 cgatagcaac tttatagaat aatgccaaca caccggcggt atagcccata tcgccagcca
     1261 ttacttcaag ctcattttga acagagggag cagccagtgc gatggcatct ttaatcgaat
     1321 gcagatcaag gcttgtacta tttaccttag cagcaataat tgccagttgc tcggcagaac
     1381 cgctgtggag taaattgtct aatgccgtca ttgccatacc tgcttccgga atgtttgaca
     1441 tcaaaccacc aaagccaata ggcagtagta aaagcggttc gaattttcgg gcgatggcaa
     1501 gccaaagcaa cactaaactg atgacaatca taatcgcttg ccccagttct aaatgcatta
     1561 gccccatacc tttaaaaagt gcaataatac tttccatacc ggactcctag gcaatactca
     1621 ttaaagtatc atttaccgcc acaacatcgc cttgtttgac attaatgcct tgaatcgtgc
     1681 cggattttgc cgcacaaatt tgggtttcca ttttcattgc ttcaagaatc agtaacacat
     1741 cgccttcagc aacttgttgc ccctctttca cttccacttt caaaatgttc cctgccattg
     1801 gagcattcac cggttcggca ttggctgata gcgtatttgc agattttggt gcagaatcga
     1861 ccgcttgcgg agccggtgtt gctggtgttg gagcgatatt ggttatatcg ccaccttcac
     1921 tcaccttcac cacaaaggct ttgccttcaa gctcaacggt ataaatggct gaaccggatt
     1981 gtgtttgggc ttttggtgca gaagcagcgg aggcttgtgc cggttttgct tcatcagcac
     2041 ttggcactgg ctcaaaggca gacggattat tgcggttttc caaatatttc cagcccactt
     2101 gttggaagag tgccacaatc aatacatcat caatcatgtt ttcagatagc gtaatgcctt
     2161 tttcttttgc acttgcagtc acttctgcca ctaatttttc cagttccggc ggaagatgat
     2221 cggctgggcg gtcggtaatc ggctctgctc cctcaagcac tctgtcttgc aaggctttgt
     2281 ttaccggtgc cggtgtgcga ccatattcgc cttttaaaat acctgcagtt tctttggcga
     2341 tagttttata acgttcgccc attaatacgt tcatcaccgc ttgtgtgccg acaatttgtg
     2401 aggttggtgt caccaacgga atattgccta aatcttcacg cacacgagga atttcttgta
     2461 aaaccaagtc taatttatcc gacgcatttt gttgcttgag ttggctttcg aggttggtca
     2521 gcatgccacc cggcacttgg gcaaccaaaa tacggctatc tataccacgg agctgtcctt
     2581 caaattttgc atatttttta cgcacgttac ggaagtaggc agagattttc tccagagctg
     2641 gaatgctcaa gccggtttca tacggcgtgc cttgaagtgt ggcgacaata gactcggttg
     2701 ccgggtgacc gtaagtgccg gacattgatg aaatggaggt atcgacacca tcgacaccgg
     2761 cttcaatcgc tttgagcaac gccatttcag ccataccggt ggttgaatgg caatggagat
     2821 gtagctcaac gtcgtagcgt tttttaattt cgctgactaa ttcataggct gccattgggg
     2881 ttaaaatgcc cgacatatct ttgatcacca acgaatccac gccaatttcc aataattgct
     2941 cggttacatc caaccacgtt tgtagggtat gaacagggct ggtggtgtaa ctcaaagtac
     3001 cttgtgcgtg cccgccgtgt tttttgaccg cttgtaatgc ggcttgcata ttgcgagggt
     3061 cgttgagggc atcaaataca cggaacacac tcataccgtt tgccaccgaa cgctcgacaa
     3121 atttatctac cacatcgtcg gcataatgac gataacccaa taagttttgc ccacgtagca
     3181 gcatttgcat tggggttttc ggcattgctt ttttcagctc acgtaaacgc acccacggat
     3241 cttcgcctaa aaaacgaata caactgtcaa aagttgcccc gccccaagtt tctaacgacc
     3301 aatagccgat attatccagc tcggaggcaa tcggtaacat atcgtctaag cgtaaacgag
     3361 tcgcaaaaag ggattggtgt gcatcacgca aaaccacatc ggtaatcgca atttttttcg
     3421 gttgagttgt cataatcaaa ttccttctaa ttattttatt tttgaaatct tgttaatgtt
     3481 gcccgttaat tcgacgatga tgagcaatcg cagcggcaat cacagggcgt aaacgctcta
     3541 aatcatctgg gggagattga ggcacagttt tagaggctgg aatagaaaca ggttcgggga
     3601 aatattgatt gataatcttg gaaataaagc tgatggcata aattaaaacc agcaggaaca
     3661 gcataacaaa tcccattccg gctaccatta aatttatacc ttccgcaaag agttctgcag
     3721 gt
//
LOCUS       DANLXF010000224         3707 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.224, whole genome shotgun sequence.
ACCESSION   DANLXF010000224 DANLXF010000000
VERSION     DANLXF010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.224"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            13..1203
                     /locus_tag="PXD66_002201"
     CDS             13..1203
                     /locus_tag="PXD66_002201"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDL6320452.1"
                     /translation="MTQAIESVLAELKRGVENIYSEEDLITKLKENRPLRIKLGADPT
                     APDIHLGHTVVLNKLRQFQQFGHEVYFLIGDFTGMVGDPSGKNSTRPPLSREDVLRNA
                     ETYKEQIFKILDPQKTKIVFNSEWLGKLGTEGMIRLASNYTVARMLEREDFKNRFTNQ
                     QPIAIHEFIYPLLQGHDSVELKADVELGGTDQTFNLLVGRELQKSAGQKPQVAMTLPL
                     LVGLDGEKKMSKSLGNYIGVTEAPSEMFGKIMSISDDLMWDWYNLLSFRPLDEIAQLK
                     ADVENGKNPRDVKILLAKEIIARFHDEAAADAAEQEFINRFQKGAIPDEVPEFTFEGE
                     IGLAALLKEAGLVPSTSEAIRSAQQGGVKIDGEKVEDVKQTAQKGTFVYQVGKRKFAR
                     ITVK"
     gene            complement(1282..1596)
                     /locus_tag="PXD66_002202"
     CDS             complement(1282..1596)
                     /locus_tag="PXD66_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HDL6320453.1"
                     /translation="MQFKDYLATFPTIDHLAGLNVINSGEVIQHIPAIEGKLGSLKLY
                     NALFAKFDGKLTASAAKQGLEWFAEHVEDAKNNVGKHPNIDLLFRVIEQDLSLSLEPI
                     VK"
     gene            complement(1678..2820)
                     /gene="nagA"
                     /locus_tag="PXD66_002203"
     CDS             complement(1678..2820)
                     /gene="nagA"
                     /locus_tag="PXD66_002203"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDL6320454.1"
                     /translation="MNHYALTNCVIYTGEQVLYQHALVVKEDKIDSIVLEENLPLDLQ
                     KIDLKGANLSAGFIDLQLNGCGGVMFNEEITTRTLEIMQETNLRSGTTSYLPTFITSP
                     DEGVKQAVAVMREYLSKHKNQALGLHLEGPYLSVAKKGVHRPEFIREISPEMLDFLCD
                     NGDVITKITIAAENPTADHIKRFNDAGIIVSIGHSNATFAQTKQRIAEGAAFATHLHN
                     AMSPVSNGREGGVVGAVLDSDIYTGIICDGLHVEWGNIRIAKKAKGDKLVIVSDAVAA
                     AGSDIESFIFVGKKVTVKDGKCYDEFGALGGAAITMIESIRNVVQHCDISLNEAIRMS
                     TLYPAKAIGVEETLGSLEAGKIANLAIFDNQFEVKATAVNGQLQWH"
     gene            complement(2901..3695)
                     /locus_tag="PXD66_002204"
     CDS             complement(2901..3695)
                     /locus_tag="PXD66_002204"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDL6320455.1"
                     /translation="MRLIPLETDHDVSVWAARYIVDRINAFQPTKEKPFVLGLPTGGT
                     PEKTYKELIKLYQAGEVSFKNVVTFNMDEYVGLPKEHPESYHSFMFKHFFDHVDIPLE
                     NINILNGMAEDVNAECERYEEKIRFYGKIHLFMGGVGVDGHIAFNEPASSLSSRTRIK
                     TLTEDTRIANSRFFNNDVSQVPKFALTVGVGTLLDAEEVMILVTGHNKALALQACVEG
                     AVNHLWTISCLQLHRRGIVVCDEPATQELKVKTVKYFKELEKNVAR"
BASE COUNT         1082 a          840 c          725 g         1060 t
ORIGIN      
        1 aaaggaagat aaatgactca agctatcgaa tcagtccttg ccgaacttaa acgtggcgtg
       61 gaaaatattt attcagaaga agatttaatc accaaattaa aagaaaaccg cccattgcgt
      121 attaaattag gggcagaccc aactgcaccg gatattcact taggtcatac tgttgtgctg
      181 aacaaacttc gtcaattcca acaattcgga cacgaagtct atttcttaat cggtgatttc
      241 accggtatgg taggcgatcc atcaggaaaa aactctaccc gtccgccact tagccgtgaa
      301 gacgtgttac gcaatgccga aacctataaa gagcagatct tcaaaatttt agatccacaa
      361 aaaaccaaga tcgtattcaa ctccgaatgg ttaggcaagt taggtacgga aggtatgatc
      421 cgcttagcct ccaactacac cgttgctcga atgttagaac gtgaagattt caaaaaccgt
      481 ttcacgaacc aacaaccgat cgcaatccac gaatttattt atccgctact acaaggtcac
      541 gactcggtag aactgaaagc tgacgtggaa ttaggtggta cagaccaaac ctttaacttg
      601 ttagttggac gtgagttaca aaaatcagcc ggtcaaaaac cacaagtggc aatgacactc
      661 ccactacttg tcggcttaga cggcgagaaa aaaatgtcga aatctctcgg caactacatt
      721 ggtgtaactg aagctccaag cgaaatgttc ggcaaaatta tgtcgatttc agacgattta
      781 atgtgggatt ggtacaacct gctctcattc cgtccattag acgaaattgc acaattaaaa
      841 gcggatgttg aaaatggcaa aaacccgcgt gatgtgaaaa ttctactggc aaaagaaatt
      901 atcgctcgtt tccacgatga agcggcagca gatgcagcag agcaagagtt tatcaaccgt
      961 ttccaaaaag gggcaattcc tgatgaagtg cctgaattta cctttgaggg tgaaatcggc
     1021 ctagcggctc tactgaaaga agccggctta gtaccgtcca cttcagaggc aattcgctca
     1081 gcccaacaag gcggggttaa aattgatggt gaaaaagtgg aagatgtgaa acaaactgct
     1141 caaaaaggca cttttgttta ccaagtcggc aaacgcaaat ttgcacgcat taccgtgaaa
     1201 taagcctctt aaacacaagc agtcattatt tgcaaaaatt ttgcagataa tgaccgcttg
     1261 ttatttttca ctagtgcaat cttatttaac aatcggctct aaacttaaag ataaatcttg
     1321 ctcaattacc ctaaataata gatcaatatt cgggtgctta cccacattat ttttcgcatc
     1381 ttcaacgtgt tcggcaaacc attccaaccc ttgttttgct gcactagcgg ttaatttccc
     1441 atcaaatttc gcaaacagtg cattgtaaag ttttagtgaa cctaatttac cttcaatcgc
     1501 aggaatatgt tgaatcactt caccgctatt tatcacgttt aagccggcta gatgatcaat
     1561 agttggaaaa gtggctaagt aatctttaaa ttgcatgtta ttctccctat aaataaaagg
     1621 cagacacaat gcctgccttt ggattatatg ctgttatgct tagaatttgt agcaaaatta
     1681 atgccactgt aattgcccgt ttacagcagt cgcttttact tcaaattgat tatcgaaaat
     1741 cgcaaggttg gcaattttgc cggcttcaag tgagccgagt gtttcctcta ccccaatcgc
     1801 ttttgccggg taaagggtac tcattcggat tgcttcattc aggctaatat cgcaatgttg
     1861 cactacgttg cgaatggatt cgatcatcgt gattgccgca ccgcccaatg ctccgaattc
     1921 atcgtagcat ttgccatctt tcaccgtgac ttttttaccg acaaaaataa acgactcaat
     1981 atcacttcct gcggcagcaa cagcatcgct gacaatcact aatttatcgc ctttggcttt
     2041 tttggcaatg cgaatgttgc cccattcaac gtgcaagcca tcgcaaataa tgccggtgta
     2101 aatatcgcta tccaacaccg cccctactac accgccttca cgcccgttgc tgaccggcga
     2161 cattgcattg tgtaaatgcg tagcaaacgc tgccccttcg gcaattcgct gtttggtttg
     2221 agcaaaagtg gcattggaat gcccgataga tacaataatt cctgcatcat taaagcgttt
     2281 gatatggtct gcggtcgggt tttctgctgc aatcgtgatt ttcgtaatta catcgccgtt
     2341 atcgcataag aaatctaaca tttcagggct gatttcacgg ataaattccg gacggtgtac
     2401 tcctttcttt gccacactta gataaggtcc ttccaaatgc aagccgagtg cttggttttt
     2461 gtgtttcgac aaatactcac gcattaccgc caccgcttgt ttcactccct catcaggcga
     2521 ggtaataaaa gtcggtaaat agctggttgt gcctgaacgc agattggttt cctgcataat
     2581 ttctaaagtg cgggtagtga tttcttcatt aaacatcacc ccaccgcagc cgttcagttg
     2641 caagtcaata aaaccggctg aaagattcgc tccttttagg tcgatttttt gcagatccag
     2701 cggtaaattt tcttccaaaa caatggagtc gattttatct tctttcacca ctaatgcgtg
     2761 ttggtaaagc acttgttcac cggtgtaaat cacacagttt gttaaggcat aatggttcat
     2821 tttctctctt ctctttaaat gctaacaggc ggtcgttttt gtaaaaaata ttacaaaatt
     2881 aaccgcttgt aaaatcagaa ttagcgagct acgtttttct ctaactcttt gaaatactta
     2941 actgttttaa ctttcagctc ttgggttgcc ggctcatcac acaccactat accacggcga
     3001 tgtaattgta agcaactgat ggtccaaaga tgatttaccg ccccttccac acacgcctgt
     3061 aatgctaagg ctttgttatg gccggtcact aagatcatca cttcttcggc atcaagcaac
     3121 gtaccaaccc caacggttaa agcaaatttc ggcacttggc ttacgtcatt gttaaagaaa
     3181 cgtgagtttg caatgcgagt atcttcggta agcgttttaa ttcgagtgcg ggaagaaagc
     3241 gaagatgcag gctcattaaa cgcaatatgc ccatccacac cgaccccgcc cataaacaga
     3301 tggattttac cataaaaacg gattttttct tcataacgtt cgcattctgc attcacgtct
     3361 tccgccatac cgttgaggat attgatattc tcaagcggaa tatcaacgtg gtcaaaaaag
     3421 tgcttgaaca taaaactgtg gtagctttca gggtgttcct taggtaagcc gacgtattcg
     3481 tccatattga aagtcaccac atttttaaag ctcacttcac cagcttgata taatttaatt
     3541 aattctttat aggttttttc aggcgtaccg ccggttggta agccaagcac aaaaggtttt
     3601 tctttggttg gttggaatgc attgatacga tcaacaatat aacgagcagc ccaaacactc
     3661 acatcgtgat cagtttctaa cggaattaaa cgcatagtct ctcctta
//
LOCUS       DANLXF010000225         3702 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.225, whole genome shotgun sequence.
ACCESSION   DANLXF010000225 DANLXF010000000
VERSION     DANLXF010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.225"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..520
                     /locus_tag="PXD66_002205"
     CDS             <1..520
                     /locus_tag="PXD66_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015483985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6320456.1"
                     /translation="NKLSDNPAIISGKQGVIFCDDLSLGVNKHRLRLRFSSPINFTVN
                     QEWQKMPFDRVLNDINGHVDIVSGQFIARTGGTFHLKGKITVTNSSEDVLNLYQLRLL
                     INNEEKERLINQSVSPKNSKSNTNGMTTINFDTVVDLKVGDIVDLQYKTTHELTLLSD
                     SFLSQIYVTQEF"
     gene            721..936
                     /locus_tag="PXD66_002206"
     CDS             721..936
                     /locus_tag="PXD66_002206"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320457.1"
                     /translation="MDLLQGEKYQWKVIKQEISLLSKQASKQASKQASKQASKQASKQ
                     ASKQSVAQFKQAPLPFTGQKRMFLKHF"
     gene            complement(1119..2714)
                     /locus_tag="PXD66_002207"
     CDS             complement(1119..2714)
                     /locus_tag="PXD66_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL6320458.1"
                     /translation="MRLSQLSLSVLLLSASSLVAAAPVNTLINCVATPPMKLSPAITN
                     DANDFNASSQQIYNRLLAFKAGELTVEPSLAERWEISDDGLSYTFHLRKDVKFHSNKQ
                     FTPTRTLNADDVVFSFQRQMDLNHPYHKVSDGTYFYYHWMNLPKILKTVEKVDDYTVK
                     ITLQQPNAPFLTTLAMDFLSIYSKEYADKLLAEGKPELLDQAPIGTGPFVFQAYQTDQ
                     AVRYVANPDYFQGKAKFEKLIFSITPDAGTRYAKLKAGECDVIDFPNISDIAQMKQDP
                     KVNLLQRDGLNLAYIGLNSTKPALNNAKVRQALHYATDKKAIVEAVYQGGGTVASNPF
                     PDSVLGYNPNLPQYEFNLEKAKALLTEAGFADGFETEIWVQPVVRPSNPNPRRTAEII
                     QADWAKIGVKAKLVTHEWADFNKRTREGEFSTGTYGWTSRNGDPDNFLFPLLSKSNIP
                     GTNYGRWTDEKFEQLLAQAVQTQNRDERAKLYQQAVEIANEQTPFIPLAHAINYVPLS
                     KRVQGFVQNPFGYTPFYGVSLSE"
     gene            2863..3303
                     /gene="mioC"
                     /locus_tag="PXD66_002208"
     CDS             2863..3303
                     /gene="mioC"
                     /locus_tag="PXD66_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HDL6320459.1"
                     /translation="MTSICIITGSTLGGAEYVADHINEVLSEQGFEVELFNQATLADI
                     EGKTNLIIVTSTHGAGELPENIQQLFDDLANSSADFSSMKFGVIGLGSSDYDTFCNAV
                     NIVEEALKAKGASQVCESLRIDVMNNFDHDGSAEEWLPSFVEQL"
     gene            3315..3482
                     /locus_tag="PXD66_002209"
     CDS             3315..3482
                     /locus_tag="PXD66_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HDL6320460.1"
                     /translation="MNKISANKAKWIKIAIILIYMFSPVDILPEAILGPFGLVDDAAA
                     LMLLIKTILEK"
BASE COUNT         1147 a          719 c          762 g         1074 t
ORIGIN      
        1 taataaatta tcggacaatc ctgcgattat atcaggtaag caaggtgtta ttttctgcga
       61 tgatttgagc ttaggagtaa ataagcacag attaagattg cgattttcat cgccgattaa
      121 ttttactgtt aatcaagaat ggcaaaaaat gccgtttgac agagtgttaa atgatatcaa
      181 tggtcatgtt gatattgtca gtggtcaatt tattgcaagg acaggcggca cttttcattt
      241 aaaaggtaaa ataacggtta caaattcatc ggaagatgtg ctgaatttat atcaactcag
      301 gctgttaata aataacgaag agaaagaaag actgattaat caaagtgttt cacctaaaaa
      361 cagcaaatca aatacaaatg gtatgacaac aatcaatttt gatactgtag ttgatttaaa
      421 agtaggtgac attgttgatt tgcagtataa aacaacgcat gagttgacat tactgtcaga
      481 ctcattttta tctcaaattt atgtgactca ggagttttaa gtgactaaaa aacactactg
      541 cacaggctgg cgtaatgcgc cagccaatat caatagttgc tgtcatcagc atgaccgaga
      601 ctacggtatc aagggcacag tcagccgagc cgaagccgac aagcggttaa gacattgctt
      661 aatttcgcaa gggaatccga ttaaggcgtg ggtgttttgg gttgtggtga ggttgtttgg
      721 gtggatttat tacaaggaga aaaatatcaa tggaaagtca taaaacagga aatatcatta
      781 ctgagcaagc aagcaagcaa gcaagcaagc aagcaagcaa gcaagcaagc aagcaagcaa
      841 gcaagcaagc aagcaagcaa gcaaagtgta gctcaattta aacaagctcc gctacctttt
      901 acaggtcaaa agcggatgtt tttaaaacac ttttaatgat gatttaaagg gcgtttgttt
      961 tcagtttatt ttgcataact tttgtgatta cggctatgca aaatgatttg caaaactatg
     1021 caaaataaat cgaccgctta caattctaaa agaaaagcag tctattctct accacatttt
     1081 gcaaaaaagt ggcaaaaata gaccgcttgt atggcatatc actccgataa actcacccca
     1141 taaaatggcg tgtagccaaa cggattttgc acaaaacctt gcacccgttt gctgagtggt
     1201 acataattga tagcgtgagc cagcggaata aatggcgttt gttcgttggc aatttccacc
     1261 gcttgttgat agagcttcgc ccgctcgtca cggttttggg tttgtacagc ttgagctaat
     1321 aactgctcaa atttctcatc cgtccaacgc ccataatttg ttcccggaat attagatttg
     1381 cttaataaag ggaagaggaa attatcagga tcaccattac ggcttgtcca accataagtg
     1441 ccggtagaga actcgccttc acgggtgcgt ttgttaaaat ccgcccattc gtgggtcact
     1501 aatttcgctt tcaccccgat tttcgcccaa tccgcctgaa taatttcagc cgttcgacgt
     1561 gggtttgggt tagaagggcg cactaccggt tgtacccaaa tttccgtttc aaatccatcg
     1621 gcaaagccgg cttctgttaa tagggctttg gctttttcaa ggttaaattc atattgcggt
     1681 aaatttggat tatagcccaa caccgaatca gggaacggat tacttgccac cgtgccaccg
     1741 ccttgataaa cagcctcgac aatcgctttc ttatcggttg cataatgcaa ggcttggcga
     1801 actttggcgt tatttaatgc cggtttggtg ctgtttaagc cgatataagc taaattcagc
     1861 ccgtctctct gtaacaaatt caccttcgga tcttgtttca tttgggcaat atcggaaata
     1921 ttcgggaaat caatcacatc gcattcaccc gctttcagtt tggcataacg agtgcccgca
     1981 tcaggggtaa tactaaaaat cagtttctca aattttgctt taccttggaa ataatccggg
     2041 ttcgcaacat aacgcaccgc ttgatcggtt tgataggctt ggaacacaaa aggccctgtg
     2101 ccgatcgggg cttgatccaa taactccggc ttgccttccg ccagcaattt atccgcatat
     2161 tcttttgaat aaatcgacaa gaaatccatc gctaaggttg tcagaaaagg agcattaggt
     2221 tgctgtaagg tgattttaac agtgtaatcg tccacttttt ccactgtttt taaaatcttc
     2281 ggcaaattca tccaatggta atagaaataa gtaccgtcag agaccttatg gtaagggtgg
     2341 tttaaatcca tctggcgttg gaacgagaac actacatcat ccgcattcag cgttcgagtt
     2401 ggcgtaaatt gtttattgga atgaaatttc acgtctttac gtaaatggaa tgtgtaactt
     2461 aagccatcgt cactgatttc ccaacgttcc gccaaactcg gctcaaccgt tagttcaccg
     2521 gctttaaacg ctaataagcg attataaatt tgttgtgaac tggcgttgaa atcattggca
     2581 tcattagtaa ttgcaggact aagtttcatc ggtggagttg ccacgcaatt aatcaacgta
     2641 tttaccggag ctgctgccac caaactgctg gcacttaaga gtaaaacaga aagagataat
     2701 tgagaaagac gcataaagac tccttaacat tttctactaa cttactcggc attaacgtat
     2761 tttcaaaata actaaaggtt ataatttata atcagaataa tcagttgcaa accaatgatt
     2821 cttggctaga atattggcag ttttttaaac gagagattta ctatgacatc aatttgtatc
     2881 atcaccggca gcaccttagg cggtgcggaa tatgttgccg atcacattaa tgaggtatta
     2941 agcgaacaag gttttgaagt ggaactgttt aaccaagcca cccttgccga tattgagggc
     3001 aaaaccaatc tgattatcgt tacctctacc cacggagccg gcgagttacc cgaaaatatc
     3061 caacagctat ttgacgattt agcaaatagc tcggctgatt tcagcagtat gaaatttggt
     3121 gtaatcggtt taggtagctc cgactatgac accttctgca atgcagtaaa tattgttgaa
     3181 gaagctctca aagcgaaagg ggcaagccaa gtttgcgaat cactgagaat tgatgtaatg
     3241 aataatttcg accacgatgg cagtgctgaa gaatggttac catcatttgt tgaacaatta
     3301 taacttaagt atcaatgaac aaaatcagtg caaataaggc aaagtggatt aaaatcgcta
     3361 ttatcttaat ctatatgttc agccctgtag atattctacc cgaagcgatt ttaggcccat
     3421 tcggtttggt tgatgatgcc gccgcattaa tgctattgat taagactatt ttggaaaaat
     3481 aggcatagta gacaaaatag ttgcaaaaaa ccaaaacaaa acgaccgctt gtaacccaat
     3541 acaagtggtc ttatctttta cttaattctt ttaagggaga atatctcttg aaataggatt
     3601 ttgaagtgaa ataagcgttt ctgttgactg aatttcatca atagactgaa tttttcctgc
     3661 taataccgaa tgcaactctt cgatggtatg ggtcattact tt
//
LOCUS       DANLXF010000226         3651 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.226, whole genome shotgun sequence.
ACCESSION   DANLXF010000226 DANLXF010000000
VERSION     DANLXF010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.226"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            5..1189
                     /locus_tag="PXD66_002210"
     CDS             5..1189
                     /locus_tag="PXD66_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HDL6320461.1"
                     /translation="MSRPLNFVMISPHFPTNFETFAIRLREAGFNTLGIADAPYESLS
                     ENLRHSLTEYYRVDNMEDYEQVYRAVAYFAHKYGRIDRIESHNEYWLELDAKLRTDFN
                     VFGYKSDDMLSIKTKAQMKEVFRKTGLKVAQGRVFKDDEDARKLAKQLKFPVIIKPNS
                     GVGASDTYKIKTAEELEAFFGYKTPTVEYIMEEFIDGDIITFDGLTDKEGNIVFYSSL
                     EYSEAVLDTVSKDGDMFYYVPCEISPKLVEMGKQCVKAFNVKERFFHFEFFRVKGSKK
                     EELLPLEINCRPPGGLTIDMWNYANDFDVFREFANIVKENKFYADIQHPWNVVYISRK
                     ANQNYVNSIEAVCEKFAGNIISVQSVPGVFAKVMGEQGILARTETMEQMREIAQFAQA
                     KQ"
     gene            1200..1952
                     /locus_tag="PXD66_002211"
     CDS             1200..1952
                     /locus_tag="PXD66_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDL6320462.1"
                     /translation="MHFEKRSHWSGELGREMSFNVYGHAGKPVIVFPSSGGNENEYGN
                     FGMIEACRSFIERGLIKFYTPDSYDAESWLSKHKSGHDMALAHNAYDRYIIHELVPLI
                     RHESHWNGTMIATGCSMGAFHTVNFALRHPDLFDIAIALSGVYDARFFSGEFYGDKAV
                     YFNSPIDYVWGQNDDWFLDRYRQNHYVIAVGQGAWEEQHIEDTRRLQEAFNAKGIDAW
                     FDYWGFDIDHDWPTWRKQMPYFLGKLEEQGII"
     gene            1973..2425
                     /locus_tag="PXD66_002212"
     CDS             1973..2425
                     /locus_tag="PXD66_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HDL6320463.1"
                     /translation="MNLKAVAVSALAMSSVAMADIADVKPVVFESKFDFQTTVTTLTN
                     TLKEKGMTIFAEIDHQAAAKQAGLEMQPATVLVYGTPKAGTPLMVKDPMLALQLPLKV
                     LITEPNKGKVEVMLNRADQVVAHANTDYADVENNLAKAEKLIKATVAK"
     gene            2529..3623
                     /locus_tag="PXD66_002213"
     CDS             2529..3623
                     /locus_tag="PXD66_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL6320464.1"
                     /translation="MKLNQAIADRIVKRTMQIIPNSVNVMDSEGVIIASGDVGRIGQR
                     HTGAVIALRNDQAVEIDENLAKQWCDEVKAGINLPLHYLGSTIGVVGISGQPQQVRQY
                     AQLVKMAAELIMQQAFELEQERWQRRYREEFVRALLKGSLSTAEIQQQAVFFGQDFAK
                     PLSVMVIKIHGSTAEKLQRLIDFFEHYFPSLLTAVIGLDKIALLNHLSNEKSLIRNAL
                     NKLNDPDISFKTVVGLAVENLSQTHISYQTACHTLNYAEQIQSRKQIIHFEDFKLPAL
                     LHSFSHSWEAKTLLSPFQALLVSDKKQVLLKSLRYYFFANCDLDHAAKKLFIHPNTLR
                     YRLEKIEQITGLSFNNIEQKFVLYLGAVLS"
BASE COUNT         1094 a          725 c          842 g          990 t
ORIGIN      
        1 aactatgtct agaccactta attttgtgat gatttccccg cacttcccaa cgaactttga
       61 aacctttgcc attcgtttgc gtgaagccgg ttttaataca ttgggaattg cagatgcccc
      121 ttatgaatca ttgagcgaaa atctgcgtca ttccctgact gaatattatc gtgtagataa
      181 tatggaagat tacgaacaag tttatcgtgc ggtcgcgtac tttgcccata aatacgggcg
      241 aattgaccgc attgaatcgc acaatgaata ttggctggag ttagatgcga aattgcgtac
      301 cgatttcaat gtgtttggtt ataagagcga tgatatgctc tcaattaaaa ccaaagccca
      361 aatgaaagag gtattccgca aaaccggctt gaaagtggca caagggcgag tatttaaaga
      421 tgatgaagat gcccgcaaat tagcaaaaca gcttaaattc ccggtgatta tcaagccaaa
      481 ttcaggggtt ggggcaagcg atacttacaa aatcaaaact gcggaagagt tagaagcttt
      541 cttcggttat aaaacgccaa cggttgaata tattatggaa gagtttattg acggcgatat
      601 tattaccttt gacggtttaa ccgataaaga gggcaatatc gttttctact ccagccttga
      661 atattcagaa gcggtattag ataccgtttc aaaagatggc gatatgttct actatgtccc
      721 atgtgaaatt tcgccaaaat tagtggaaat gggcaaacaa tgtgtgaaag cctttaacgt
      781 aaaagagcgt tttttccatt ttgagttctt ccgtgttaaa gggagcaaaa aagaagagtt
      841 gttaccactc gaaatcaact gccgtccgcc gggtggctta actattgata tgtggaacta
      901 cgccaacgat ttcgatgtgt tccgtgaatt tgccaatatt gtgaaagaaa acaaattcta
      961 tgccgatatt cagcaccctt ggaacgtggt ttatatttcc cgcaaggcaa accaaaatta
     1021 tgtgaattcg attgaagctg tttgtgagaa atttgccggc aatattatca gcgtgcaaag
     1081 cgtaccgggc gtatttgcca aagtgatggg cgaacaaggc attttagccc gtaccgaaac
     1141 aatggagcaa atgcgtgaaa ttgcacaatt tgcacaagct aaacagtaag aggtgaagta
     1201 tgcattttga aaaaagaagc cactggagtg gagaattagg tcgtgaaatg tcgttcaacg
     1261 tttacggaca cgcaggtaaa ccggttattg ttttcccttc ctccggcggc aatgaaaacg
     1321 agtatggtaa ttttggaatg attgaggctt gccgttcctt tattgaacgt ggcttaatca
     1381 aattttacac gcctgattct tacgatgcgg aatcttggtt atctaaacat aaatccggac
     1441 acgatatggc attagctcat aatgcttacg accgttatat tattcacgag ctagtgccgt
     1501 taatccgcca tgaatcgcac tggaacggca cgatgattgc caccggttgt agtatggggg
     1561 ctttccatac ggtaaacttt gccttacgtc atccggatct gtttgatatt gccatcgcct
     1621 taagcggtgt ttatgacgct cgctttttca gtggtgagtt ttatggcgat aaagcggttt
     1681 attttaactc gccgattgat tatgtatggg gtcaaaatga cgattggttc ttagaccgct
     1741 accgccaaaa tcactatgtg attgcagtcg gacaaggtgc gtgggaagaa cagcatattg
     1801 aagacacccg ccgattacaa gaggctttta acgcaaaagg gatcgatgct tggtttgact
     1861 actggggctt tgatattgac catgactggc cgacttggcg taaacagatg ccttatttct
     1921 taggcaagtt ggaagaacaa gggattattt aatctatttg aggagaaaag aaatgaactt
     1981 aaaagcagtc gcagtttctg cattagcaat gagcagcgtg gcaatggcag atattgccga
     2041 tgtcaaaccg gtggtatttg aaagtaaatt tgatttccaa actacagtca caactttaac
     2101 caacacctta aaagaaaaag gaatgacgat ttttgccgaa atcgatcacc aagcggcggc
     2161 aaaacaagcc ggcttagaaa tgcaaccggc aaccgtattg gtttacggca caccgaaagc
     2221 cggtacgcca ttaatggtga aagatccgat gttagcgttg caattaccgt taaaagtgtt
     2281 aattaccgaa ccgaacaagg gcaaggtcga agtaatgtta aatcgtgccg atcaagtggt
     2341 ggctcacgct aataccgatt atgctgatgt agaaaacaac cttgccaaag cggaaaaatt
     2401 aattaaagcg actgtggcaa aataacttca cgctatgatc tattctcagg aagtggaagc
     2461 gtagataatt tgcaaatccc tcgaaagagg gatttttcat agtattggga atgagtagga
     2521 atcatataat gaaactcaac caagcgattg ccgaccgcat cgtcaagcgc acgatgcaga
     2581 ttattcccaa ctccgtcaat gtgatggata gtgaaggggt gattattgct tccggcgatg
     2641 tcggtcgtat cgggcaacga cacacaggag cggtgattgc gttacgcaac gatcaagcgg
     2701 tcgaaattga tgaaaatctt gcaaaacagt ggtgcgatga agtgaaagcg ggcattaact
     2761 taccgcttca ctatttaggc tcaaccattg gtgtagtggg gatttcaggg cagccacaac
     2821 aagttcggca atatgctcaa ttggtgaaaa tggcggcgga gctgattatg cagcaagcgt
     2881 ttgagttgga acaggaacgt tggcaacgcc gctatcggga agagtttgtg cgggccttgc
     2941 tcaaaggtag cctaagcaca gcagaaatcc agcaacaagc ggtctttttc gggcaagatt
     3001 ttgcaaaacc gctgtcggtg atggtgatta aaattcacgg ctctactgcg gaaaaactcc
     3061 aacgcctgat tgattttttc gaacattatt tcccgagcct actcaccgcc gtgattgggt
     3121 tagataaaat tgccttgctg aatcatttat ccaacgaaaa atcgttaatc cgcaatgcac
     3181 taaacaaact taacgatccg gatatttcct tcaaaacggt ggttgggctg gcggtcgaaa
     3241 atttatcgca aacccatatt tcctaccaaa ccgcctgcca taccctcaat tatgccgagc
     3301 agatccaaag ccgtaagcag attattcatt ttgaagattt taaactgccg gccctcttgc
     3361 acagtttcag ccactcttgg gaagcgaaaa ccttgctctc gccgttccaa gctttactgg
     3421 tttccgataa aaaacaggtg ttactgaaaa gtctgcgtta ctattttttc gcaaattgtg
     3481 atctggatca cgctgccaaa aaattgttta ttcacccaaa tacgctacgt taccgtttag
     3541 agaagattga gcagataact ggtttatctt tcaataacat agagcaaaaa tttgtgctgt
     3601 atcttggagc ggttctgtcg taaaaattgt aaaaatatac aaacaagcgg t
//
LOCUS       DANLXF010000227         3651 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.227, whole genome shotgun sequence.
ACCESSION   DANLXF010000227 DANLXF010000000
VERSION     DANLXF010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.227"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            1..2034
                     /locus_tag="PXD66_002214"
                     /pseudo
     CDS             1..2034
                     /locus_tag="PXD66_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249230.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
     gene            2078..3514
                     /locus_tag="PXD66_002215"
     CDS             2078..3514
                     /locus_tag="PXD66_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3327 domain-containing protein"
                     /protein_id="HDL6320465.1"
                     /translation="MKKTTACILTFLVSSVLYANNITIELKQNDFVQGKLIQKAQQPL
                     SLSIIFPDQSSRLLAQNVFGEQDFMFKSELIGTATFSILANGATVSDQDFTLTIERHI
                     PISAQIAPPKVIENTQLEELSQKIAKNPEKQTALINDFWDKAKQAGTPLIEPLDEQQS
                     RVTFLWRGATKNARIWGGVSTEHDFMQRFGETDIWYKSYIVPNDTLVEYKLAPDVPTL
                     PVDENTQRRALLATAQADPLNKTAYFEKIGQNIHNTDKFNYASLLKLPNAPTQSFLTE
                     APNIAKGKLQQLIFESATLGNKHRLFVYLPDGFSAEKNYAVLYLFDGVEYTERVPTKT
                     ILDNMIAQKVILPTVAVFIENPSSESRRVELPANPIFTQVLAKELVPFVEKQLNIKTK
                     ERIIGGSSYGGLSSAYTVLNYPNVFSKALILSGSFWWQPKGTPSEQSHYIAQQFIQKE
                     KLPLCFFISAGFYESGKSAICKRSNYFA"
BASE COUNT         1202 a          737 c          690 g         1022 t
ORIGIN      
        1 attgtagctc ctcaagtatt agcagaaaca acctctcaag cagaactaaa tgctattgag
       61 gtcaaagaga aattggcagc taccaccggc tatattcctg tttatacgac tgtaggaacg
      121 aaaacattaa cctctttggc tcgtacacct tttagtatca atgttgtctc tgatgattta
      181 atgcatgacc aagctgttcg ttctgttacc gattcagtgg cttatacctc cggtatgatg
      241 ggtggctatc gtggtaacaa ttcgcttatc gaaatctcca ttcgaggcat tggcaataaa
      301 agtgatggca gtacaattcc gacctattta aacggttctc gctaccaaac tgcatttgaa
      361 atcgatccgt tcttcttaga aagtatcaat gtgattaaag gtccaaactc cattttatac
      421 gggcaagcta atcccggtgg cgtaatggat attatcacca aaaaagccgg cagtaaccca
      481 catcgccatt tgcaatttgt ggtgggaaac catcaacgat accaaatcgg gctggatatt
      541 gaagaaaatc tctccaatac cttatccgct cgtttaatcg gtcaattaga acgaacaaac
      601 tggaatacct aatttgtaaa agaaaaaggc ggtgcttttg ctccaagcat tttatggaaa
      661 ccgactacaa atacagaact taatgtttac gcttactatg caaaaaaacc gaaagccggt
      721 gatcgtaact ttttaccgaa agaaggaacg attaatagcg taaacgggaa ggaactaccc
      781 tatgatatgt tcctcagcga tctgaactat cacggtttaa aagccgaaca aatgcaagta
      841 ggttacagtt tctcacataa atttacgcca aacctaacgt tccgccaaaa tgcaagttac
      901 cataatggcg aagagaattt caaaaatcta gtttattggg gcgatgtcgg ttcggtttta
      961 gaacgtagag ctcgccattg ggatgtcaaa acccaagaaa tcaacttaga taaccaatta
     1021 gaatgggtat ttagcacagc aaatataaat cataccttgt tagccggtat tgattataaa
     1081 cgagtgcgta aagatcttga taactatatg gggattgccc ctgattttga ttggcagaac
     1141 cctgtttatg gcgtgaccgt tactgagcct agcttatttt caagtgaagt gaagcattta
     1201 aaacaacttg gggtttatct acaagatcaa atagaaatcg gtaattttga cttcctgtta
     1261 ggcggtcgtt acgatcgagc aaaaaacagc aaccaagatc gtttgaacag taaaacagag
     1321 aataaccaag aaggaaaatt tacttggcgt acaggcgtag tttacaactt tgaaaacggt
     1381 atcgctccgt atatcagcta cagcacttct tttgtgccat cggctgaaaa agatgctcaa
     1441 ggaaatttct taaaaccaac aactgctggg caatgggaag tgggaatgaa ataccaacct
     1501 acccaagaca ccttactgac cttagcagga tttaaaatta cacaaaaaga tttagccaat
     1561 tatcaatggc aaacccgctc ttatgagcaa attggtgaag tgcaaaccaa aggaattgaa
     1621 gtgagtttaa atcagcaatt atccgataaa ttttcaatag cggcttctta tgcttactta
     1681 aaaaaagaga ttactgcaga taaagatggc acgacgattg gaaaaacgca gtggggagtg
     1741 cctcgccatc aagcatctct ttgggcaaaa tataaattct ttgataagct gaccgccggt
     1801 gtaggtgtac gctatatggg aacgactttt ggcgataacc aaaatacttt taccgtaccg
     1861 gcatacacac tttacgatat gatgctagct tatgatttta tgcctaactt aaacttacag
     1921 ctcaacgcac aaaacctgac gaataaaaaa tatgtcgcaa gctgtgccaa taatttttcc
     1981 tgtttctatg gaaaagatcg caatttaagt gcaacgttaa actatcattt ttaatctcaa
     2041 cagccctcaa atgagggctt aaacttttgc aaaaaatatg aaaaaaacga ccgcttgtat
     2101 tcttactttt ttggtttcat ctgtgcttta tgctaacaat ataaccattg agcttaaaca
     2161 gaatgatttt gtgcaaggca aattgattca gaaggctcaa cagccacttt ccttgtcgat
     2221 tatttttcct gaccaaagct ctcgcctgct ggctcaaaat gtctttggtg aacaagattt
     2281 tatgtttaaa tcagaactaa tcggcacagc tactttttct attctagcga atggtgcaac
     2341 cgtttcagac caagatttta ccttaacgat tgagcggcat attccaatct cggcacaaat
     2401 tgctccacct aaagtaattg aaaatacaca gcttgaagaa ctcagccaaa aaattgcaaa
     2461 aaatccagaa aaacagaccg ctttaattaa cgatttttgg gataaagcaa aacaagccgg
     2521 cacaccattg attgaaccat tggacgaaca gcaaagccgt gtaacttttt tatggcgtgg
     2581 agcaacgaaa aatgcccgta tttggggcgg tgtgagtact gaacacgatt ttatgcaacg
     2641 ctttggtgaa acggatatat ggtataaaag ttatatagtg cccaatgaca ctttagtgga
     2701 atacaaatta gctccagatg tgccgacatt gcccgttgat gagaataccc aacgcagagc
     2761 gttactggct acggctcaag ccgatccact caataaaaca gcatacttcg agaaaatcgg
     2821 acaaaatatt cataataccg ataaattcaa ctacgcttcg ctacttaaat tgccaaacgc
     2881 acctactcaa tcttttttga ctgaagctcc aaatattgca aaaggtaaac tgcagcagtt
     2941 gatttttgag agtgcaacac tcggcaataa acacagatta ttcgtctatc tgcctgacgg
     3001 ttttagtgca gaaaaaaact atgcggttct ctatttattt gatggtgttg aatataccga
     3061 acgagtgcca actaaaacca ttttagataa tatgatcgct cagaaagtga ttctgcctac
     3121 tgtagcggtg tttatcgaaa atccatcgag tgaatcaaga cgtgtcgagc tgcctgccaa
     3181 tcccatattt acgcaagtat tggcaaaaga acttgtacct tttgttgaaa aacagctcaa
     3241 tataaaaaca aaggagcgaa ttattggcgg ctctagctat ggcgggctat cctctgctta
     3301 caccgtactc aactatccta atgtatttag taaagcattg attctatccg gttcattttg
     3361 gtggcaaccc aaaggcacac ctagtgaaca aagccactat attgctcaac aatttatcca
     3421 aaaagaaaaa ttaccactct gttttttcat cagtgcaggt ttttatgaaa gcgggaaatc
     3481 ggctatttgt aagcggtcga attattttgc atagaattac actcaatttt gcatagataa
     3541 aaattaaaaa ctatgcaaaa taattgtaga agatcaaacc taatctgaca gtcccccgtt
     3601 ttaaattacc gtgtctgtca gattaatttg agcttaaatt cttttccacc c
//
LOCUS       DANLXF010000228         3629 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.228, whole genome shotgun sequence.
ACCESSION   DANLXF010000228 DANLXF010000000
VERSION     DANLXF010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.228"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(90..2654)
                     /locus_tag="PXD66_002216"
     CDS             complement(90..2654)
                     /locus_tag="PXD66_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HDL6320466.1"
                     /translation="MRIAKIIFSALLSLLVLGGVIGGLLYMHFKSDLPDVATLKNVEL
                     QQPMQIFTLDGKLIGEVGEERRIPVKLDDIPPMLVKAILATEDARFYEHKGIDPKGIM
                     RAIWRTSQGDTQGASTITQQLAKNFFLTPERSLERKAKEAILALEIEQVLSKNEILEL
                     YLNKIYLGYRSHGVAAAAKTYFNKSLKDLTLSEIAIIAGLPKAPSTMNPLYSVKRAED
                     RRNVVLGRMLETGDITKEEYEKAKAEPVVANYYGATLEFRADYVTEMVRQEIVKRYGE
                     NIAYNKGLKVYATVLSADQKAAQDALRENLIDYDRRHGWRGAEKLWTGSSAWDDEKII
                     DHLSKLPKSEPFTPAVVMQADKSKYTILLANGDISVLKRTNASFAPKGLKAGEQIWVR
                     QNKNREWALGQIPEVNSALVSINSENGAIESIVGGFSFEQSRFNRATQSLVQVGSAIK
                     PFIYTAAMNKGLSLSTTISDSPIVIKKKGQKEWRPKNADGVYGGPLRTRVALGKSKNM
                     VAIRILQMAGIDYVADYLQRFGFNRNQYVATESLALGAASFTPLEMARAYAVFNNGGY
                     LIEPYIIDRIIDAQGYELYQANPVVACNSCDSPVVYPEPKYFESVKIHDEAQVGVQTI
                     ANSSNNDDDDVVEDVGDSEPELQANNSEKNAPSLMAESSHHIKGLRYAPHVISDELAF
                     LMRSALATAITGEPEYGWRGTSYKMLNTIKRADVGGKTGTTNNAKATWYAGFGANIST
                     VVYVGFDDNKRVLGRGASGSSTALPAWTNYMKVALSDKPVEKSTLPKNIIEVNIDPNS
                     GFLGNGRKEYFIKGTEPTKRYIVERAYTEKAPQKTESAPLRLGLPPPGVLKSGELF"
     gene            2778..3476
                     /locus_tag="PXD66_002217"
     CDS             2778..3476
                     /locus_tag="PXD66_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComA"
                     /protein_id="HDL6320467.1"
                     /translation="MKLLKAHSKTPSNRIILGLSEDEQRYCLVKNKAEKFTVFWQAKP
                     ASIEELIGNLTPQEQAMMTLVRPIPYQYIWRKTVFMANTLNEAQLHRKVIHILKNEQP
                     LALDLLNIDYQRFPSSNDNLDKVVIYAVRKSYTENLSQFNSILDCELHCYARAIIHLT
                     QSQNNQPAPCFSFKQKYFQFTETELLILQTEPEDCIHLSDLKIDHLEIECEQEKQLYC
                     LALGASLWNGKVLI"
     gene            3452..>3629
                     /locus_tag="PXD66_002218"
     CDS             3452..>3629
                     /locus_tag="PXD66_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320468.1"
                     /translation="MEWQGIDLSANRKNNWFAGSDKGIKNVIYTLLLSALISLALIFL
                     YLQKNQEIQPLAQQL"
BASE COUNT         1096 a          807 c          605 g         1121 t
ORIGIN      
        1 caaatcgccc aaaataaaat tggcacttat tatcgcaaga aatcccccta tttttcaact
       61 tttaagagca ttatccttgt aataaattat tagaataatt caccgctttt tagcacccct
      121 ggtggaggta atcctaaacg taacggcgca gattctgttt tttgaggcgc tttttcagta
      181 taagctctct ctacaatata acgttttgta ggctcagtac ccttaataaa atactcttta
      241 cgtccatttc ctaagaaacc actattaggg tcaatattaa cctcaataat attttttgga
      301 agggtacttt tttcgacagg cttatctgac aatgcaactt tcatataatt tgtccaagct
      361 ggtaaagcag tactagaacc tgaagcacct ctacctaata ctcttttatt atcatcaaag
      421 ccaacgtaca caacagtaga aatattagca ccaaatcccg cataccaagt tgccttggca
      481 ttattggttg taccagtctt cccgcccaca tccgctcgtt ttatcgtatt gagcatttta
      541 tagcttgttc ctcgccagcc atattcaggc tcaccagtaa ttgctgtagc taaagcacta
      601 cgcattaaaa aagcgagttc atcactaata acatgaggag cgtaacgtaa ccctttgata
      661 tgatgagaag attctgccat taagctcggc gcatttttct cgctattgtt cgcttgtaat
      721 tcaggttctg aatcgccaac atcttccaca acatcgtcgt catcattatt cgaggaattc
      781 gctatagttt gtacaccgac ttgagcttca tcatgaattt tgactgattc aaaatattta
      841 ggctcagggt aaacaactgg gctatcgcaa ctattacaag ctactacagg atttgcttga
      901 tataactcgt agccttgggc atcaataata cggtcaatga tatatggttc aatcaaataa
      961 cctccattat taaatactgc ataagctcga gccatttcta atggcgtaaa agatgcagca
     1021 cccaatgcaa gagattctgt tgcaacatac tgatttcggt taaagccaaa acgttgtaag
     1081 tagtctgcaa cataatcaat ccctgccatt tgtaatatac gaatagccac catatttttc
     1141 gatttaccta atgcaacacg agttctcaac gggccgccat aaaccccatc ggcattttta
     1201 ggtcgccact ctttttgccc tttcttttta atgacaatag gagaatcgct aattgttgtt
     1261 gaaagactta gccctttatt cattgccgct gtgtaaataa acggtttaat cgcagagcct
     1321 acctgaacta atgactgagt cgcacgattg aaacggcttt gttcaaagct aaagcctcca
     1381 acgatagatt caatggcacc gttttcacta ttaattgaca ctagggctga atttacctcc
     1441 ggaatttgtc ctaaagccca ctccctattt ttattttgac gtacccaaat ttgttcacct
     1501 gcttttaacc ctttaggtgc aaaagaagca tttgtacgtt ttaaaacaga gatatcacca
     1561 tttgccaata aaattgtata ttttgattta tctgcttgca ttacaacagc tggtgtaaac
     1621 ggctctgatt ttggcagctt acttaaatga tcgataattt tctcatcgtc ccaagcagaa
     1681 ctacctgtcc ataatttttc agcgcctcgc caaccatgac ggcggtcata atcaatcaaa
     1741 ttttcacgta acgcatcttg tgctgctttt tggtctgctg aaagtaccgt ggcgtacact
     1801 tttaagcctt tgttataagc aatattttct ccatagcgtt tcacaatttc ttgacgaacc
     1861 atttctgtca cataatccgc tcgaaattct aatgtcgcac catagtagtt agctactact
     1921 ggctctgctt tagctttctc atattcctct tttgtaatat ctccggtttc tagcattctc
     1981 ccaagcacta cattacgacg gtcttccgcc cgttttactg aataaagtgg attcatcgtt
     2041 gaaggcgctt ttggaagccc tgcaataatt gctatttcag aaagggttaa atctttcaaa
     2101 gatttattaa aataagtttt agccgcagcg gcaacaccgt gagaacgata ccctaggtaa
     2161 attttgttta aatacagctc cagaatctca tttttactga gaacctgttc aatttctaaa
     2221 gctaaaattg cctctttggc cttacgctct aaactacgtt caggtgttaa gaaaaagttt
     2281 ttcgccagct gctgggtgat tgtacttgcg ccttgagtat ctccctggct agtgcgccat
     2341 attgcacgca taataccttt aggatcgatc cctttatgct cgtaaaaacg tgcatcttcg
     2401 gtagcaagaa tcgcttttac taacatcggt ggaatatcat caagcttgac cggaatacgg
     2461 cgttcttctc ctacttcacc aattaattta ccatctaagg taaaaatctg cataggctgc
     2521 tgtaactcta catttttcag cgttgccaca tcaggcaaat cagatttgaa atgcatgtac
     2581 agtaaaccac caattacccc acctaaaacc aagagtgaaa gtaaagcact aaatattatt
     2641 tttgcgatcc gcatcgaaaa aatctctttt ggttaatgac gaaacataaa tttaaagcca
     2701 atattaggtc actaagtata agctaaaaac gcttacttcg cataactaat tgcaaaactt
     2761 tccttactat acttactatg aaattactta aagcacattc aaaaacacct tcaaaccgta
     2821 tcatcttagg cttaagtgaa gatgaacaac gttactgctt agtcaaaaat aaagcagaaa
     2881 aattcaccgt tttttggcaa gccaagcctg cgagcatcga agagcttatc ggtaatctca
     2941 ccccacaaga acaagcaatg atgacgctgg ttcgcccgat cccttaccaa tacatttggc
     3001 gaaaaaccgt ttttatggct aacaccctca atgaagctca attacaccgt aaggtaattc
     3061 acattttaaa aaatgagcag ccacttgcat tggatctact caatattgac tatcagcgat
     3121 ttccaagcag caacgataat ctggataaag tggttatcta cgccgtgcga aaaagttata
     3181 ccgagaattt gagccaattt aacagcatct tagattgtga gctgcattgc tatgcacggg
     3241 ctataattca tttaacacaa tcccaaaata atcagcctgc gccctgtttt tcatttaaac
     3301 agaagtactt ccaatttacc gagacggaat tactgatatt gcaaacagaa cctgaggatt
     3361 gtattcatct atctgatctc aaaattgacc atcttgaaat cgagtgtgag caagagaaac
     3421 agctatattg cctcgcctta ggagcaagtt tatggaatgg caaggtattg atttaagtgc
     3481 taatcgaaaa aataactggt ttgccggcag tgataaaggc atcaaaaacg tgatttatac
     3541 acttttatta tctgccctca ttagcttggc tctgattttt ctctatttgc aaaaaaatca
     3601 agaaattcaa ccgcttgcac agcaattag
//
LOCUS       DANLXF010000229         3620 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.229, whole genome shotgun sequence.
ACCESSION   DANLXF010000229 DANLXF010000000
VERSION     DANLXF010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.229"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            131..493
                     /locus_tag="PXD66_002219"
     CDS             131..493
                     /locus_tag="PXD66_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDL6320469.1"
                     /translation="MKPTNKADFQRVINAAGYSMKGLKAAYINEAAFRQEIWCATILF
                     PLGLILGETNIEKALLVGTVLLVLVTELLNSAVESVVDRIGADFHELSGRAKDIGSAA
                     VFMAMVLLAITWILILFC"
     gene            530..2173
                     /locus_tag="PXD66_002220"
     CDS             530..2173
                     /locus_tag="PXD66_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HDL6320470.1"
                     /translation="MRLKKTLLSVALLSATSAMAYETGKTYRFTVLHTNDIHGHFWQS
                     DKGEYGLAAQKTLVDNIRKEVAEKGGSVLMLNAGDANTGVPESDMQNARPDIEGMNAI
                     GYDAMVLGNHEFDYPLQILSMQEKWAKFPFISANVINKKTDKPIVKPYVMLERGGLKI
                     AVIGLTTEDTAKLGNPDVTENAFFKNPIETAKNTLAEINQKEKPDVRIALTHMGYYFD
                     GKHGTNAPGDVTMARTLEKGAFDLIIGGHTHDTVCIDEKGQFKAKYTPGEECKPDFQN
                     GTWIVQAGEWGKFVGRADFEFKDGKATLVKYELIPVNLKKTIKKEDGTKEYRLYQPEI
                     KADEATYTLLKKYQDEGDKKLNIEVGRVKGLLDGKREHIRFQQTNLGRLIAQSQMERV
                     KADIGIMNSGGIRTSLEEGVITYKHILTVQPFGNMIATVDLKGQELLDTLTTIALKEV
                     DTGAYPQFAGISMVVDRTAKTISDVKIGGKPFDKEKTYKVSLPDYVAAGGDGYPLLKK
                     HPSYVNTGFIDAEMLKKFFEENKIIEADKYDPKNQIIFK"
     gene            complement(2380..3249)
                     /locus_tag="PXD66_002221"
     CDS             complement(2380..3249)
                     /locus_tag="PXD66_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL6320471.1"
                     /translation="MSSRGKILNTISSLQNSLTKTEKKIAAAILSQPELLSQCSLSEV
                     AKQLDVGEATFIRFCRTLGFKGYTDFKLELAIELATQNQDNRVLLDTDVSESDTSKDI
                     AEKLKVSLDNVIEETINLLDFNVLEKVVEELRKAKRIFLFGVGSSGLTAEDAKHKLMR
                     IGLQTDAVTNNHFMYMQAALVKEGDLVIGISHSGYSEEIVKSLRFSRANKATTVAITH
                     NLRSPVTEEANYVLINGNRQGHMQGDSIGTKMSQLFVLDLIYTLLVKAEPENALKQKQ
                     KTLNVILEQRVKG"
     gene            complement(3259..>3620)
                     /locus_tag="PXD66_002222"
     CDS             complement(3259..>3620)
                     /locus_tag="PXD66_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDL6320472.1"
                     /translation="LGWQELENGIRMNVMSFETTPAEGKKAELHRKFIDIQLLIDGEE
                     MIEYGLSQPDLSKYDEYRDEDDYQLTAEIDDKNNLILKPNMFAIFLPYEPHKPGNSVN
                     GNKTLKKLVVKVPVELI"
BASE COUNT         1151 a          697 c          649 g         1123 t
ORIGIN      
        1 gattttttta caaaaaagtg aagtttttta cattccatgg ctatcatatt attttttcct
       61 taggttcttt gaacatcacc attctttcag tacaatagtt ctctaaactc ttttatttat
      121 tttatttact atgaaaccaa ccaataaagc agattttcaa cgtgtgatca atgcagctgg
      181 ctactctatg aaggggttaa aagccgctta tattaatgaa gccgcctttc gccaagaaat
      241 ttggtgcgct actattttat tccctctagg gcttattcta ggcgaaacca atattgaaaa
      301 agcattatta gtaggcacag ttttacttgt tttagttaca gaactactca atagtgcggt
      361 tgaatctgtt gttgaccgca ttggggctga tttccacgag ctttctggta gagctaaaga
      421 tattggctct gcagctgtat ttatggcgat ggttttactc gctataactt ggatattaat
      481 tttgttttgt taactctatt tttcaaccaa cactcttact gaggtattta tgcgcttaaa
      541 aaaaacatta ctttcggtag cactacttag tgcaacatca gcaatggcct acgagacagg
      601 taaaacatat cgttttacgg ttttacatac taacgatata cacggtcact tttggcaaag
      661 tgataaaggc gaatacggtc tcgctgctca aaaaacattg gttgataata ttcgcaaaga
      721 ggtcgcagaa aaaggtggtt cggtattaat gctgaatgca ggtgatgcga atacaggcgt
      781 gccggagtca gatatgcaaa atgcacgtcc tgatattgaa ggaatgaatg cgattggtta
      841 cgatgcaatg gttctaggta atcacgaatt tgattaccca ctgcaaattc tctctatgca
      901 agaaaaatgg gctaaattcc cctttatttc ggccaatgtc attaataaaa aaacggataa
      961 acccattgta aaaccttacg tgatgttaga acgaggcgga ctaaaaattg ctgttatcgg
     1021 attaacaacc gaagataccg caaaattagg taatccagat gtaacggaaa atgccttttt
     1081 caaaaaccct attgaaacag caaaaaacac attagctgaa attaatcaaa aagaaaaacc
     1141 cgacgtacgt atcgctttaa cccatatggg ttactatttt gacggtaaac atggtactaa
     1201 tgcaccaggt gatgtaacaa tggcccgtac cttagaaaaa ggagcgttcg atttaatcat
     1261 tggcggtcat acccacgata ctgtttgtat tgatgaaaaa ggtcaattca aagccaaata
     1321 tacgccgggt gaggaatgta aaccagactt ccaaaacggt acttggattg tgcaagccgg
     1381 cgaatggggc aaatttgttg gacgggctga ttttgagttt aaagatggaa aagctacctt
     1441 ggtaaaatat gaattaattc cggtcaactt aaaaaaaacc attaaaaaag aagacggtac
     1501 taaagaatac cgactttacc agcctgaaat caaagccgat gaagcaacct ataccttatt
     1561 gaaaaaatat caagatgagg gcgataaaaa gcttaatatt gaagttggtc gtgtgaaagg
     1621 gttattagac ggcaaacgtg agcatattcg cttccaacaa accaatctcg gtcgtttaat
     1681 tgcacaatcg caaatggaaa gggtaaaagc cgatatcggg attatgaact ccggcggtat
     1741 tcgtacctcc cttgaagaag gtgtaataac ttataaacat attctgactg ttcaaccttt
     1801 cggtaatatg attgcaacgg tggatttaaa aggacaagaa ttgttagata ccctcaccac
     1861 tattgctctg aaagaagttg atactggcgc ttatcctcaa tttgccggca tttcaatggt
     1921 agtcgaccgt acagcaaaaa ctatttctga tgttaaaatc ggtggcaaac cttttgataa
     1981 agagaaaact tataaagttt ctctgcctga ctatgttgcg gctggtggcg atggctaccc
     2041 attactgaaa aaacacccta gctatgtcaa tacaggcttt attgatgccg aaatgctgaa
     2101 aaaattcttc gaagaaaata aaattattga ggcagataaa tacgatccaa aaaaccagat
     2161 cattttcaaa tagagctata gaaaatgaaa ctcactctgg cagtctctgc tcaaaagagc
     2221 tgtggctgcc tgtagattgt tttcttactt tcaagtgtta taggaaatcg ataaaaaatc
     2281 cctaccaaat taattcatca ccatatgatg aaaattaaaa aacctctggc aatactttgc
     2341 caaaagtctt tgacttttat agggtaatat cactgcttat tagcctttaa cacgttgttc
     2401 caaaatcaca ttcagtgttt tttgtttctg tttcaacgca ttttcgggtt cggctttaac
     2461 tagtaaggta taaattaaat caagaacgaa aagttgtgac attttagtac cgatagaatc
     2521 tccttgcata tgcccctgtc gattaccatt aattaacaca taattagcct cttccgtgac
     2581 gggggaacgt aaattgtgtg taatcgcaac cgttgtcgct ttatttgcac gagaaaaacg
     2641 tagtgatttc acaatttctt cagaatagcc tgaatgacta atccctataa ctaaatcccc
     2701 ctctttcacc aatgccgcct gcatatacat aaaatgatta tttgtgactg catcggtttg
     2761 tagcccaata cgcattaatt tatgttttgc atcttctgcg gttaacccag aagaacccac
     2821 tccaaataaa aagatccgtt ttgccttacg taattcttca acaacttttt ccaacacgtt
     2881 aaagtcaagc aaattaatag tctcttcaat aacgttatct aaactcactt tgagcttctc
     2941 agcaatatct ttcgatgtat ccgattctga aacatccgta tcaagcaata ctctattatc
     3001 ttgattttgc gtagccaact ctattgcaag ctctaattta aagtcggtat aacctttaaa
     3061 acctaatgta cgacaaaaac gaataaaagt tgcttcacca acatctaatt gtttggccac
     3121 ttcagataat gaacattggc taagtaattc cggctgagat aaaatagcag cagctatttt
     3181 cttctctgtt ttggttaaac tattttgtag cgagctaatt gtattaagaa tttttccccg
     3241 actagacata gtagcctcct aaatcaattc aacaggtact ttcaccacca gttttttcaa
     3301 tgttttgttg ccattcaccg aatttccagg cttatgtggt tcataaggga ggaaaatagc
     3361 gaacatattt ggcttgagga tcaagttatt tttatcatca atctcagctg ttaattggta
     3421 gtcatcttca tctcgatatt catcatactt agataaatct ggctggctta acccgtattc
     3481 aatcatttct tcgccgtcta tcaataattg aatatcgata aattttcggt gtaactctgc
     3541 ttttttcccc tcagctggcg tagtttcaaa tgacattaca ttcatgcgaa taccattttc
     3601 taattcttgc cagcctaatt
//
LOCUS       DANLXF010000230         3582 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.230, whole genome shotgun sequence.
ACCESSION   DANLXF010000230 DANLXF010000000
VERSION     DANLXF010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.230"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(10..2862)
                     /locus_tag="PXD66_002223"
     CDS             complement(10..2862)
                     /locus_tag="PXD66_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDL6320473.1"
                     /translation="MVQAVSFSHFFAIHYFYFFLHKTKGQCTAKPLFSILLFHLFPKN
                     SAKFLLFFYFLVLMVLYSNEVANGKLLITGGTNLNGTLSAEGTSEIILSGRPTPHAYD
                     KVANKEVLIEGDWINRSFNATIFAAKDNGKLEISRNVANINGNFNLTDNATAQIGFTQ
                     GTSQACIRSDRTGVATCNINATLSETDLNSWERTKVAGNVSLADNSTFSLGSKADLTG
                     SITAQESTKVQLNDGSIANLTGLSTTGIFNGANGSTVNLSGVWTTLTNTVVDVLNVAN
                     GSQIHLNTPTATKQNTLTANTLTGNGIFKFVADLTKTASDIVTIKDTASGQFSIEIST
                     NGSAHSAQKIKLFETTNEEDFSLNLANEVRSGEYRYHLLKEGNVYYLNPEKEEEPKIT
                     QKGPSETADPLPEYTGDIEHSEKGPGETADPLPEYTGDIEHSEKGSGETAEPLPKYNG
                     NAHLIASELTAQTNAVLGVTTVLDHVIHSEGTQKQNTLTANTLTGNGIFKFVADLTKT
                     ASDIVTIKDTASGQFSIEISTNGSAHSAQKIKLFETTNEEDFSLNLANEVHSGEYRYH
                     LLKEGNVYYLNPEKEEEPKITQKGPSETADPLPEYTGDIEYSEKGPGETADPLPEYTG
                     DIEHSEKGPGETADPLPEYTGDIEHSEKGSGETAEPLPKYNGNAHLIASELTAQTNAV
                     LGVTTALDHVIHSEGTQNWKVWSKTDYQTTEHRSAGYGFNQDANVIHLGLEKALDNQV
                     TLGMILSQSKARNNFDHYYSGKGRLTMLSMYAKKTWQNGVFVAIDTGFGKASNRLTYQ
                     ANTVKLDRSVFVTGLSIGKAWESANVNIIPSFSARYHHLSSAGNQLVDAKIETNAVDL
                     LALQAGLSINKTLELNGLQIKPEIGSYFVDASHGKLRTRFNNLQIEQQMGRYFKQEAG
                     ISANYRNINAGIQAGFLTGNTLNKQRYISFKVTYEW"
     gene            complement(2910..>3582)
                     /locus_tag="PXD66_002224"
     CDS             complement(2910..>3582)
                     /locus_tag="PXD66_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL6320474.1"
                     /translation="SIIRKEWFTASEVAGMGNLPEQPSNVTRRATKNNWLKRQIEGKR
                     GVAFEYHYSSLPISVQQELGFAQTAVKEEPRTLVAAQTADEMERVPFYNVQASAGFGA
                     FNSEVYAPDDYIGLSKRWLDVRGFYLSYLAFITASGDSMYPTISDGDMLLVNRATTQP
                     KDGKIYVFRQGEQLWVKRVQGIMGGIRLISDNKELYSPIDVIFDETLDFEVIGQVVYI
                     GHEMI"
BASE COUNT          946 a          741 c          697 g         1198 t
ORIGIN      
        1 tctcaatatt taccattcat atgtaacttt gaacgagata taacgctgtt tatttagcgt
       61 attccccgtt aagaaacctg cctgaattcc tgcgtttata ttgcgataat tagcggatat
      121 acctgcttct tgtttaaaat aacgccccat ttgctgctct atttgtaaat tattgaaacg
      181 tgttcttaat ttcccatggc ttgcatcaac aaaatagcta cctatttccg gttttatttg
      241 caggccattt agttcaagcg ttttattaat tgacagacca gcttgaagag cgagaagatc
      301 aacagcgttt gtttcgattt ttgcatctac aagttgatta cctgctgaag ataaatgatg
      361 ataacgggcg ctaaatgatg ggattatatt cacgtttgca gattcccatg ctttaccgat
      421 actaagccct gttacaaata ctgaacgatc taattttact gtatttgctt gataagttaa
      481 gcgattgctt gctttcccaa aaccggtatc tatcgcaaca aaaacaccat tttgccatgt
      541 tttcttcgca tacattgata gcattgttaa gcgaccttta ccgctataat aatggtcaaa
      601 attatttctc gcttttgatt gagataatat cataccaagt gtgacctgat tatctaaggc
      661 tttctctaat cctagatgga taacgttcgc atcttggtta aatccatatc cggcagagcg
      721 atgctctgtc gtttgataat ctgttttaga ccatactttc caattttggg tcccttccga
      781 atgaattaca tgatctaatg ctgtagttac acctaataca gcattagttt gcgcagttaa
      841 ctcggatgcg atcaaatgtg catttccatt gtatttaggt aatggctcag cggtttcacc
      901 cgatcctttt tctgaatgct caatgtctcc tgtgtattca ggtaacggat cagcagtttc
      961 acccggccct ttttccgaat gctcaatgtc tcctgtgtat tcaggtaacg gatcagcagt
     1021 ttcacccggc cctttttcag aatactcaat atccccggtg tattcaggta atgggtcagc
     1081 ggtttcactt ggcccttttt gagtaatttt aggttcttcc tctttctctg gattgaggta
     1141 atacacattc ccttctttga gtaaatggta gcgatattcg cctgagtgca cttcatttgc
     1201 taaattaaga gaaaagtctt cttcatttgt tgtttcaaat aatttaatct tttgggctga
     1261 atgggcgcta ccatttgtgc taatttctat cgaaaattga ccgcttgcgg tgtctttaat
     1321 tgtgactata tcgctcgctg ttttcgtcag atcagcgaca aatttaaata tgccgttccc
     1381 tgtgagggta ttagcggtaa gcgtattctg tttttgggtc ccttccgaat gaattacatg
     1441 atctaatact gtagttacac ctaatacagc attagtttgc gcagttaact cggatgcgat
     1501 caaatgtgca tttccattgt atttaggtaa tggctcagcg gtttcacccg atcctttttc
     1561 tgaatgctca atgtcgcctg tgtattcagg taacggatca gcagtttcac ccggcccttt
     1621 ttcagaatgc tcaatatccc cggtgtattc aggtaatggg tcagcggttt cacttggccc
     1681 tttttgagta attttaggtt cttcctcttt ctctggattg aggtaataca cattcccttc
     1741 tttgagtaaa tggtagcgat attcgcctga gcgcacttca tttgctaaat taagagaaaa
     1801 gtcttcttca tttgttgttt caaataattt aatcttttgg gctgaatggg cgctaccatt
     1861 tgtgctaatt tctatcgaaa attgaccgct tgcggtgtct ttaattgtga ctatatcgct
     1921 cgctgttttc gtcagatcag cgacaaattt aaagatgccg ttccctgtga gggtattagc
     1981 ggtaagcgta ttctgttttg ttgcagttgg agtgtttaaa tggatttggc taccatttgc
     2041 gacattcaag acatctacaa cagtgttggt taaggttgtc cataccccag aaagattaac
     2101 ggttgaacca ttggcgccat taaatattcc tgttgtactc aagccggtta aattagcaat
     2161 acttccatca ttaagttgaa ccttagtgga ctcctgagct gtaattgagc cagttaagtc
     2221 tgctttactg cctaacgaaa atgttgaatt gtcagctaaa gagacattgc cagccacttt
     2281 ggttctctcc cagctgttta aatcagtctc tgataaagta gcgttaatat tacaggttgc
     2341 tacgcctgtg cggtcagagc gaatacatgc ttgtgaggta ccttgggtaa aaccaatttg
     2401 agcggtagca ttatctgtta aattaaaatt accgttaata ttggcaacat tacggctgat
     2461 ttcaagcttg ccattgtctt tagctgcaaa gattgttgcg ttgaacgatc ggttgatcca
     2521 gtcgccttca atcaggactt ctttattcgc tactttatca taggcatgag gtgttggacg
     2581 gcccgataaa atgatttcgc ttgtaccttc tgcagaaagc gtaccgttga ggttcgttcc
     2641 cccagtgata agcaatttgc cgtttgctac ttcattactg tatagaacca ttaaaactaa
     2701 gaaataaaag aataatagga atttggcgga atttttcggg aataagtgaa acagaagtat
     2761 tgaaaacagg ggctttgcgg tgcattgccc ctttgtttta tgtagaaaaa agtaaaaata
     2821 atgaattgcg aaaaaatggc taaaactaac cgcttgtacc attaaaacaa cgaaaaagta
     2881 aaaatgattt aagtagcttt aaatggcgtt taaatcatct cgtgtccaat gtacacaacc
     2941 tgcccgataa cctcaaaatc taacgtttca tcaaaaatca catcaatagg gctgtatagc
     3001 tctttattat cgctgattaa gcgaataccg cccataatgc cttgcacacg tttaacccaa
     3061 agctgctcgc cttggcggaa cacataaatt ttaccgtctt taggctgggt ggtggctcgg
     3121 tttaccaata gcatatcgcc atcactgatg gttgggtaca ttgaatcacc tgaagcggta
     3181 ataaatgcaa gatagctcaa atagaagcca cgcacatcta gccatcgttt acttagccca
     3241 atatagtcat caggggcata cacctcgcta ttgaaagcac caaagcctgc tgaagcctgt
     3301 acattataaa acggcacacg ttccatttca tctgcagttt gggctgccac taatgttcta
     3361 ggctcttctt ttactgccgt ttgagcaaag cctaactctt gctgcacgct tattgggaga
     3421 gagctgtagt ggtattcaaa agcaaccccc ctttttccct ctatttgtct tttgagccaa
     3481 ttatttttgg tagctcttct tgttacattt gatggttgtt caggtaaatt tcccattcca
     3541 gctacttcag atgccgtaaa ccattccttt ctaattatag aa
//
LOCUS       DANLXF010000231         3500 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.231, whole genome shotgun sequence.
ACCESSION   DANLXF010000231 DANLXF010000000
VERSION     DANLXF010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.231"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            44..1846
                     /gene="frdA"
                     /locus_tag="PXD66_002225"
     CDS             44..1846
                     /gene="frdA"
                     /locus_tag="PXD66_002225"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HDL6320475.1"
                     /translation="MQSVNFDVAIIGAGGGGLRAAIAAAEANPNLKIALISKVYPMRS
                     HTVAAEGGSAAVIKDTDSFDNHFNDTVGGGDWLCEQDIVEYFVEHSPIEMTQLERWGC
                     PWSRREDGEVNVRRFGGMKIERTWFAADKTGFHILHTLFQTSIKYPNIVRFDEHFVLD
                     ILTDNGEARGCVAMNMMEGTLVQINANAVVIATGGGCRTYRFNTNGGIVTGDGLSMAY
                     RHGVALRDMEFVQYHPTGLPNTGILMTEGCRGEGGILVNKDGYRYLQDYGLGPETPIG
                     KPENKYMELGPRDKVSQAFWQEWRKGNTLKTAKGVDVVHLDLRHLGEKYLLERLPFIC
                     ELAKAYEGVDPAKAPIPVRPVVHYTMGGIEVDMNAETSIKGLFAVGECASSGLHGANR
                     LGSNSLAELVVFGKVAGENAARRALEVTARNQPQIDAQAKDVVARLHALANQEGNESW
                     SDIRNQMGDAMEEGCGIYRTQESMEATVNKIQELKERYKNISVKDKSSVFNTDLLYKI
                     ELGFILDVAQSIACSAVERKESRGAHQRLDYVERDDVNYLKHTQAYYNAEGTPTIKYS
                     DVKITKSQPAKRVYGAEAEAQEKVKKAAEAQAQQ"
     gene            1862..2593
                     /locus_tag="PXD66_002226"
     CDS             1862..2593
                     /locus_tag="PXD66_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HDL6320476.1"
                     /translation="MANLDKMTIEVLRYNPESDSEPHLDRYEVPYDSQTSLLDALGFI
                     KDELEPELSYRWSCRMAICGSCGMMVNGKPKLACKTFLRDYSGFMRIEPLANFPIERD
                     LVVDLSHFIDSIEAIKPYIIDNKAPEGQRTKQTPAQLEKYRQFSMCINCGLCYAACPQ
                     FGLNPEFIGPAAITLAHRYNLDNRDNGREERMKLLSSKNGVWSCTFVGYCSEVCPKHV
                     GPASAVNQGKLESAKDYVISMIKPR"
     gene            2603..2995
                     /gene="frdC"
                     /locus_tag="PXD66_002227"
     CDS             2603..2995
                     /gene="frdC"
                     /locus_tag="PXD66_002227"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HDL6320477.1"
                     /translation="MSATKRRPYVRGMKASWWKKLDFYKMYMVREATCLPTVWFCIVL
                     FYGAVALSKGTFTTDFVDFLQNPLVVILNIISLAAMLYHAATLFVMTPAVMSVMVKGK
                     HLPVSVGRNILWAATGVISVIALILVYI"
     gene            3009..3356
                     /gene="frdD"
                     /locus_tag="PXD66_002228"
     CDS             3009..3356
                     /gene="frdD"
                     /locus_tag="PXD66_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HDL6320478.1"
                     /translation="MSLDQNPKRSNEPPVWLLFSAGGMVSALFFPVVIFIIGLLLPFG
                     LVSPDNIISFAHTWIGKLAIMVLAIFPMWAGMHRIHHGLHDLKVHTPAGNVIFYGLSI
                     LYTILVIIAVVQI"
BASE COUNT         1026 a          640 c          837 g          997 t
ORIGIN      
        1 ttacttttta atgaattaag ttatttaact ttcggagtgc attatgcaaa gtgttaattt
       61 tgatgtcgcg attataggtg ccggtggtgg tggcttgcgt gctgctattg cggcggctga
      121 agcaaatcca aatctaaaaa ttgctttaat ttcaaaagtt tacccaatgc gtagccatac
      181 ggttgcagca gaaggtggtt cagcggcggt aattaaagat accgactctt tcgacaatca
      241 ctttaatgat accgtcggtg gtggtgactg gttatgtgag caagatattg tggagtattt
      301 tgtagagcat tcgccgattg aaatgacaca attagaacgc tggggttgcc cttggagtcg
      361 tcgtgaagac ggtgaagtga atgttcgccg ttttggagga atgaaaattg agcgtacttg
      421 gtttgcggcg gataaaaccg gtttccacat tttgcatact ttgttccaaa cttcgattaa
      481 atatccgaat attgtgcgtt ttgatgagca ttttgtgctg gatattctca ccgacaacgg
      541 cgaagctcgt ggttgtgtgg caatgaatat gatggaaggc acacttgttc aaatcaatgc
      601 aaatgcagtt gtgattgcaa ccggtggcgg ctgccgtact tatcgcttta acactaacgg
      661 cggtattgta accggcgacg gtttatcaat ggcgtatcgt cacggtgtgg ctttgcgtga
      721 tatggagttc gtacaatatc acccaacagg tttgccaaat accggtattt tgatgacaga
      781 aggttgtcgt ggtgaaggtg gtattttagt taataaagat ggctaccgtt acctgcaaga
      841 ttatggttta ggaccggaaa cacctatcgg caaaccggaa aacaaatata tggaattagg
      901 ccctcgtgat aaggtttcac aagcattctg gcaagaatgg cgtaaaggga acaccttaaa
      961 aaccgcaaaa ggcgtggacg tagttcactt agatttacgc catttaggtg aaaaatattt
     1021 acttgagcgt ttaccgttta tttgtgaatt agcgaaagcc tatgaagggg tcgatccggc
     1081 taaagcgccg attcctgttc gcccggtggt tcactacacg atgggtggta ttgaagtgga
     1141 tatgaatgcg gaaacctcaa ttaaaggctt attcgcagta ggtgaatgtg cttcatcagg
     1201 cttgcacggt gcgaaccgtt tgggttctaa ctcattagca gagcttgtgg tgttcggcaa
     1261 agttgccggt gaaaatgcgg ctcgccgtgc gttagaagtg actgctcgca accagccaca
     1321 aattgatgca caagcaaaag atgtggtggc tcgcttgcac gcattagcga accaagaggg
     1381 taatgaatct tggtcggata tccgtaacca aatgggtgat gcgatggaag aaggttgtgg
     1441 tatttaccgt acccaagaaa gtatggaagc gactgtaaac aaaatccaag agctaaaaga
     1501 gcgttataaa aacatcagcg taaaagataa atcaagtgtg tttaacactg atttactcta
     1561 caaaattgaa ttaggcttca ttttagatgt ggctcaatcc attgcttgct ctgcggtaga
     1621 gcgtaaagaa tctcgtgggg ctcaccaacg tttagactat gttgagcgtg atgatgtgaa
     1681 ctacttaaaa cacacacaag cgtactacaa tgcagagggt acaccgacga ttaaatacag
     1741 cgatgtgaaa atcactaaat cacaaccggc taaacgtgtg tatggtgcag aggctgaagc
     1801 ccaagaaaaa gtcaaaaaag cagcagaagc tcaagcacaa caatagagaa ggagtagtaa
     1861 aatggcaaat ttagataaaa tgaccatcga agtgctacgc tacaaccctg aatcagatag
     1921 cgagccacat ttagacagat atgaagtgcc gtatgacagc caaacttcac ttttagatgc
     1981 attgggcttc attaaagacg aattagaacc ggaactttct tatcgctggt cttgccgtat
     2041 ggcgatttgt ggttcttgcg gaatgatggt aaacggtaag ccgaagttag cttgtaaaac
     2101 attcctgcgt gattacagcg gttttatgcg aattgagccg ctggcgaact tcccgattga
     2161 gcgtgactta gtagtggatt taagccactt tattgatagc atcgaggcaa ttaaacctta
     2221 tattattgat aataaagcac cggaaggaca gcgtactaaa caaacgccgg cacaattaga
     2281 gaaatatcgt caattctcaa tgtgtatcaa ctgcggttta tgttatgcgg cttgcccaca
     2341 atttggtttg aaccctgaat ttatcggacc tgctgcgatt acattggctc accgctataa
     2401 cttggataac cgtgataatg gtcgtgaaga gcgtatgaag ctactcagca gcaaaaacgg
     2461 tgtttggagc tgtactttcg tgggttactg ctcggaagtc tgtccgaaac acgtcggccc
     2521 tgcttcggct gtaaaccaag gtaaacttga aagtgcgaaa gattatgtta tctcaatgat
     2581 caaaccgaga taggaggaag aaatgtcagc aacaaaacgt agaccttatg taaggggaat
     2641 gaaagccagc tggtggaaaa aactcgactt ctataaaatg tatatggtgc gtgaagctac
     2701 ctgtcttcca actgtttggt tctgcattgt attattttat ggagcagttg cgttaagtaa
     2761 aggaacattt actaccgatt ttgtggattt cttacaaaat ccgttagttg ttatcctaaa
     2821 cataatttca ttggcagcaa tgttatatca tgcggcaaca ctttttgtga tgacacctgc
     2881 cgtcatgtcc gtgatggtta aaggtaaaca cttaccagtc tcggttggaa gaaatattct
     2941 ttgggcagca acaggcgtaa ttagcgtaat tgcattaatc ttggtttata tttaagaagg
     3001 ggaagaaaat gagtcttgat caaaacccaa aacgttcaaa tgaaccgccg gtatggttgc
     3061 tttttagtgc tgggggaatg gtaagtgcat tatttttccc tgtggtaatc tttattatcg
     3121 gtttattact gccattcggg ttagtatcac cagataatat catttccttt gcccatactt
     3181 ggatcggcaa gctcgctatt atggtattag cgattttccc tatgtgggca ggaatgcacc
     3241 gtatccatca tggattacac gacctgaaag ttcacacgcc agcaggtaat gtgattttct
     3301 acggattgtc tatcttatac acaattctag taatcatcgc agtcgtgcag atttaaatga
     3361 gaaagaccat cttgtataaa gcagatggtc ttttcatttt taatatgcta acaagcggtc
     3421 gaatttataa gaaaatttgc aaatattaga aattttgccg ataaaacgat cgttttgtat
     3481 taaatatctg caaaaaagat
//
LOCUS       DANLXF010000232         3408 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.232, whole genome shotgun sequence.
ACCESSION   DANLXF010000232 DANLXF010000000
VERSION     DANLXF010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.232"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            202..1185
                     /gene="epmA"
                     /locus_tag="PXD66_002229"
     CDS             202..1185
                     /gene="epmA"
                     /locus_tag="PXD66_002229"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HDL6320479.1"
                     /translation="MNNLQLDNIDWQPTASIPHLIQRSKIITEIRKFFTDRGLLEVET
                     PILSEFSVTDVHLSTFNTQFQSPFSPEAKTLHLMTSPEYHMKRLLAAGSGPIFQLCKV
                     FRNEEAGKRHNPEFTMLEWYRPHFDMYRLINEVDDLLQQILDCEPAESFSYQFVFQTY
                     VGLDPLSATKAQLVEKARKHGLQCDDDEQRDTLLQFLFSEVVEANIGQERPTAVYHFP
                     STQAALAQISSEDHRVAERFEFYYKGLELCNGFHELDDADEQIHRFEQDNLQREKMGL
                     EPQQLDVRFLAALKAGFPNCSGVALGVDRLLMLAMDAEKIEEVISFGIEKA"
     gene            1267..2157
                     /gene="rarD"
                     /locus_tag="PXD66_002230"
     CDS             1267..2157
                     /gene="rarD"
                     /locus_tag="PXD66_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDL6320480.1"
                     /translation="MNISGKLQGWHYALLCYLTWGTFPIYWYPINQSGMPAEQILAQR
                     VLWSALFAVFLLLIFQQGRAVINAFKQPKVLGLFVLSSFMIGVNWLVYLWAIVNEHIL
                     DASLGYFINPIFNVFVGRLILKEQLNKAQLLALLFAISGILWLAIPAGQIPWVALILA
                     ASFCIYGLIRKLAPMEPLSGLALETLLMSPFAITYLCFCYHQNILVFSELNPLQMAVL
                     LCSGIATTLPLLWFAMGARRISMSLLGMLQYISPTLQFLCGVLIFNETLSAERAVGYI
                     LVWIGVGVFLLGMKKAGVKR"
     gene            complement(2168..2779)
                     /locus_tag="PXD66_002231"
     CDS             complement(2168..2779)
                     /locus_tag="PXD66_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL6320481.1"
                     /translation="MKYEAKPLIISPNHIQQRLHGKLLARALLIIALLMLAKFIYIAS
                     LSNYFTFYLMEKFQLNIAMAQLSLFTFLGAVALGAFVGGPIGDKIGRNAVIWFSFLGM
                     APFALLLPHADLFWTIVCAIFAGFIMSSAFSAMVVYAQEAVPGRVGLISGTMFGLMFG
                     IGGLSAAVLGFFADEYGLESIFKACAYLPLLGFAALGLPKRNI"
     gene            complement(2807..3342)
                     /locus_tag="PXD66_002232"
                     /pseudo
     CDS             complement(2807..3342)
                     /locus_tag="PXD66_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251153.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         1014 a          727 c          701 g          966 t
ORIGIN      
        1 aaggtaataa agcttcataa aggtattgag aatggctctt aattggacag tgatagaaag
       61 aaaatttcta atgtttaaaa tgagaaaatt taatttgttg caaaaaagtt aaaaccctct
      121 ctttattttt cttgatcggt aactagggca actcactttt ttaggctaaa atagcctcac
      181 attttcaagc ggtcattttt tatgaacaat ttacaactcg ataatattga ttggcaacca
      241 acagcctcta ttcctcacct gatccaacgt tcaaaaatca ttacggaaat ccgcaagttc
      301 tttaccgatc gagggctttt agaggtagaa accccgattt taagcgaatt ttcggtgaca
      361 gacgttcatc tttcgacttt caatacgcag tttcaatcgc ctttttcccc tgaggcgaaa
      421 acactccatc tgatgaccag cccggaatat catatgaagc gactacttgc tgctgggagt
      481 ggaccgattt tccaactgtg taaagtattt cgcaacgagg aagccggtaa gcgacacaac
      541 cctgaattta cgatgctgga gtggtatcgc ccgcattttg atatgtatcg cttaatcaat
      601 gaagtggatg atttactgca acaaattttg gactgcgaac cggcagaatc attcagctat
      661 caatttgtgt tccaaaccta tgtcggatta gatccgcttt ctgccaccaa agcacaactt
      721 gtggaaaaag cccgaaaaca cggcttgcaa tgtgatgatg atgaacagcg tgacaccttg
      781 ttacaatttt tattcagcga ggttgtcgaa gccaatatcg ggcaagaaag accaactgcc
      841 gtttatcatt tcccttccac ccaagcggca ttagctcaaa ttagctcgga agatcaccgt
      901 gttgccgaac gttttgagtt ttactacaaa ggcttggaac tctgtaacgg cttccacgaa
      961 ttagacgatg ctgatgagca aattcatcgc ttcgagcaag ataatctgca acgtgaaaaa
     1021 atggggctgg agccacagca actagatgtg cgttttcttg ccgcactcaa agcgggtttt
     1081 ccaaactgct caggtgtcgc ccttggggtt gataggctgt taatgctggc aatggatgcg
     1141 gaaaaaattg aagaggttat ttctttcggc attgaaaaag cctaattttt tatgccgttt
     1201 tgtgatttgc aatttgcaaa aaaatcgaca aatatgaccg cttgtacact cgtttaggaa
     1261 agcaaaatga atatttcagg caaattacag ggttggcact atgccctact gtgctactta
     1321 acttggggaa cctttccgat ttattggtat ccgatcaacc aatcgggaat gcctgccgag
     1381 caaattttgg ctcaacgggt gctttggtct gccctatttg ctgtgtttct gctgctgatt
     1441 ttccaacaag ggcgagcggt tattaatgcc tttaaacagc caaaggtact cgggctgttc
     1501 gtgctttctt cttttatgat tggcgtgaac tggctggttt acttatgggc aatcgtcaat
     1561 gagcatattt tagatgccag tctaggttat tttatcaacc ccattttcaa cgtgtttgtc
     1621 ggtaggctta ttctaaaaga gcagctgaat aaagcccaat tattggctct gctgtttgca
     1681 attagcggca ttttatggct tgctattcct gccggacaaa ttccgtgggt cgcattgatt
     1741 ctagccgcca gtttctgtat ttacgggctg attcgtaaac ttgccccaat ggaacctttg
     1801 tctggtttgg cgttagaaac tttgctgatg tcgccttttg ccatcaccta tctctgcttt
     1861 tgttaccacc aaaacatttt ggtattcagt gaactcaatc ctttacaaat ggcagtgcta
     1921 ctctgctccg gcattgccac taccttacca ttgctatggt ttgcaatggg tgcaagacgc
     1981 atttcaatgt cgctactcgg tatgctgcaa tatatttcac ctaccctgca atttttatgc
     2041 ggtgtgctga tttttaatga gacactttcc gccgaacgtg ctgtaggcta cattctggtt
     2101 tggattgggg tgggggtttt cttattgggt atgaaaaaag ctggtgtcaa acgctaaaaa
     2161 taaaacgtta aatatttcgt tttggtagcc ctaatgcagc aaaaccaagt agtggcaaat
     2221 aggcacaggc tttaaaaata ctctccaagc catattcatc cgcaaaaaaa cccaatacgg
     2281 cggcacttaa gccgccaata ccaaacatta aaccgaacat tgtgccggaa atcagcccta
     2341 ctctgcctgg tacggcttct tgagcataaa ccaccatagc ggagaacgct gatgacataa
     2401 taaaaccggc aaaaatcgca cacacaatcg tccaaaagag atctgcgtga ggcaacaaca
     2461 aagcaaaagg tgccatacct aagaaagaga accaaatcac cgcatttcgt ccaatcttat
     2521 cgccaatcgg cccgccgaca aaagcgccta aagcaactgc acctaaaaag gtaaatagag
     2581 agagctgtgc cattgctata ttgagttgga atttttccat taaataaaag gtgaaatagt
     2641 tactgaggct cgcaatataa ataaatttcg ctaacatcaa taaagcgata attaataatg
     2701 ctctggctaa taatttaccg tgtaagcgtt gttgaatatg gtttggtgaa ataatgagcg
     2761 gtttggcttc atatttcacc caacggctta ctttatataa aacccatcat aatgccaata
     2821 atacaaatgc aacaacccaa atcaccgcac tttgccctaa cggcacaata aataatgcaa
     2881 ctaagagcgg acctaacgcc gatccactgt ttccacctac ttggaaaagc gactgagctg
     2941 taccaaattt accgcccgat gccattcgag ccacccgaga ggcttcaggg tgaaaaatgg
     3001 cagagcctat ccccattaac gcagcggcta tcagtaacac gccaaaattc ggcgaaaagg
     3061 caagtaaaat cacggaaaga agcgtaatca gcatactgac gggcaataaa ttcggttttg
     3121 ggtatcggta taaagcccaa tacccggctg aataatagat gcggtaattt gataaatgag
     3181 tgaaatcata ccaatttgag caaaactcag ttggtaattt tcttttagca ttggatagat
     3241 cgaaaccatt actgcttgaa ctaaatcatt caacaaatga gaaagggaaa tcagcagtaa
     3301 aatgggcaaa accatttttt gagcgttatg ttgtgtgtgc attctttatt cgttttttat
     3361 aaatgaaaaa tattctactc gcaaataatg aatgtgcaag cggtcaaa
//
LOCUS       DANLXF010000233         3389 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.233, whole genome shotgun sequence.
ACCESSION   DANLXF010000233 DANLXF010000000
VERSION     DANLXF010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.233"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            8..322
                     /locus_tag="PXD66_002233"
     CDS             8..322
                     /locus_tag="PXD66_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HDL6320482.1"
                     /translation="MKKLTPDEAIDIAYDIFLEMAGENLDPADILLFNLQFEERGAVE
                     MVETSEDWDQEIGTLIDPDAFAEVWVGLVNEQDEMDDVFARFLISHDEENREYHVVWK
                     EK"
     gene            335..847
                     /gene="hpaC"
                     /locus_tag="PXD66_002234"
     CDS             335..847
                     /gene="hpaC"
                     /locus_tag="PXD66_002234"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HDL6320483.1"
                     /translation="MANIDTNQPIEASLKFREAMAHLSSAVSIVTTNGVAGKAGLTVS
                     SVCSVTDSPPTLLFCINKNSHVHDIIQQNGKVCVNVLSHEQEDLAKHFAAILESTMEE
                     RFGWDIWNNGFDDQPVLRSAISALQGNIVDTHAVGTHTIFVVEVSHIDVEPNHSLVYF
                     ARQFRSVNID"
     gene            965..1837
                     /gene="tehB"
                     /locus_tag="PXD66_002235"
     CDS             965..1837
                     /gene="tehB"
                     /locus_tag="PXD66_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HDL6320484.1"
                     /translation="MENLICYKQMPVWTKATLPQMFQEKHNTKVGTWAKLTILKGSLK
                     YYELTEEGEIIKEDIFDANHQRPMVEPQLWHKVEALSDDLECQLAFYCKPEDYFAKKY
                     NLTATHSEVLNAVNYTKGGKALDLGCGRGRNALYLNLLGFDVTAVDHNQESIDFLNYM
                     IEKEQAQNITTAVYDINEATIGSQNGEYDLIISTVVMMFLNRERIPAIIENMQKNTKV
                     GGYNLIVCAMSTDDYPCPMPFSFTFKEGELADYYQGWELVKYNEDLGHLHKTDENGNR
                     IQLRFATMLAKKIS"
     gene            1975..3012
                     /gene="purM"
                     /locus_tag="PXD66_002236"
     CDS             1975..3012
                     /gene="purM"
                     /locus_tag="PXD66_002236"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDL6320485.1"
                     /translation="MSNTQLSYKDAGVDIHAGNELVERIKPDVKRTRRPEVMGGLGGF
                     GALCALPTKYKEPILVSGTDGVGTKLRLAIDLNKHDTIGQDLVAMCVNDLVVQGAEPL
                     FFLDYYATGKLEVDVATNVVKGIADGCVISGCALVGGETAEMPGMYHEGDYDLAGFCV
                     GVVEKSKIIDGSTVKTGDALIALASSGPHSNGYSLIRKVLEVSRSNPATDLLEDKPLS
                     EHLLAPTKIYVKSILKLVEEVDVHAIAHLTGGGFWENIPRVLPESAKAVIDESSWQWP
                     AIFSWLQEKGNISRYEMYRTFNCGVGMVIALPERDVEKALTLLKESGENAWLIGKIED
                     LGTGSEQVEIR"
     gene            3090..3368
                     /locus_tag="PXD66_002237"
     CDS             3090..3368
                     /locus_tag="PXD66_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HDL6320486.1"
                     /translation="MQLFWPIYFVLMNLAALYLMYSDKQKAIKKEWRVPEITLLSFCL
                     LGGAIGVYLGMKYFRHKTQHWYFHVAVVLSTFAWLIGLPYFYLALSSY"
BASE COUNT         1028 a          658 c          715 g          988 t
ORIGIN      
        1 taacgaaatg aaaaaattaa cccctgatga agccattgat attgcctacg atattttctt
       61 agaaatggca ggcgaaaatt tagatcctgc cgatatttta ctgttcaacc tccaatttga
      121 agagcgtggt gcagtggaaa tggtcgaaac ttctgaagac tgggatcaag aaatcgggac
      181 tttaattgac cctgatgcct ttgccgaagt ttgggttggc ttagtcaatg aacaagatga
      241 aatggacgat gtattcgccc gtttcttaat ttcccacgat gaagaaaacc gtgaatatca
      301 cgttgtttgg aaagaaaaat aaaaaggaga aaaaatggct aatatcgaca ctaatcaacc
      361 aatagaagcc tcgcttaaat ttcgtgaagc aatggctcac ttatcttctg cggtgagcat
      421 tgtgacaacc aatggcgtag ccggcaaagc agggctgacg gtttcgtctg tttgttccgt
      481 taccgattcc ccaccaacgc tcttattttg cattaataaa aacagccacg ttcacgatat
      541 tatccaacaa aacggcaaag tatgcgttaa tgtgttaagc cacgaacaag aagacttagc
      601 aaaacatttt gccgccattt tagaaagtac aatggaagaa cgctttggtt gggatatttg
      661 gaacaacggc tttgacgatc agcctgtgtt acgcagtgct atttctgctt tacaaggcaa
      721 tattgttgat acgcacgcag tgggtacgca tactattttt gtggtagaag tgagccatat
      781 tgacgtagag ccgaaccaca gtttagttta ttttgcccgc caatttagaa gcgtgaacat
      841 tgactaaaat atatttaaca gaaatttgat caaatccgac ttttttagtc ggatttttta
      901 tttcctgttt ttaaaacagt gctaaaattt tgccgtttat gtttgactga caaaaggaaa
      961 caaaatggaa aatttaattt gctacaaaca aatgccggtg tggacaaaag caaccttacc
     1021 acaaatgttc caagaaaagc acaacaccaa agtcggaact tgggcaaaac tcaccattct
     1081 caaaggttct ctcaaatatt atgaattaac cgaagaggga gaaattatca aggaagatat
     1141 ttttgatgca aaccaccaac gcccaatggt tgagcctcaa ctttggcata aggttgaagc
     1201 tctttctgac gatttagaat gccagttggc tttctattgc aagccggaag attattttgc
     1261 taaaaaatat aatctgaccg ctacccattc agaagtgcta aatgccgtca attataccaa
     1321 aggtggcaaa gcgttagatt taggttgcgg gcgaggacgc aatgcacttt atctgaattt
     1381 actgggtttt gatgtcactg ctgttgatca taatcaagaa agcattgatt ttctcaacta
     1441 tatgatcgaa aaagaacaag ctcaaaacat tactaccgct gtttatgata ttaacgaggc
     1501 aactatcggc tcgcaaaacg gtgaatatga cctgattatt tccactgttg taatgatgtt
     1561 cttaaaccgt gaacgcattc ctgccattat tgagaatatg cagaaaaaca ctaaagtcgg
     1621 cggttataac ttaatcgtat gtgcaatgag taccgatgac tacccttgcc cgatgccgtt
     1681 ctcctttacc ttcaaagaag gcgagctcgc cgattattat caaggttggg aattagtcaa
     1741 atataacgaa gatttaggac atttacacaa aactgatgaa aacggcaacc gcattcaact
     1801 acgttttgcg acaatgttgg cgaaaaaaat cagctaaact taaccgcttg cagggggaat
     1861 tagcttcccc ctaattcatc aaatccattc tatttatttt cactttttca ttccgcaaac
     1921 gtttgcttgt gctataatgg cttcgttttt tagtcattta ctgttgaggt taaagtgagc
     1981 aacacacaac taagttacaa agatgccggc gttgatattc acgcaggaaa cgagttagtc
     2041 gaacgaatca agcctgatgt taaacgcacc cgtcgccctg aagtaatggg cggattaggt
     2101 ggttttggtg ctttatgtgc tttaccgaca aaatataaag agccgatttt agtttccggt
     2161 accgatggcg tcggcacaaa attacgctta gccattgact taaacaaaca cgatactatc
     2221 gggcaagatt tggtagcgat gtgtgtgaat gaccttgtcg tgcaaggtgc tgaaccgctt
     2281 ttcttcttag attattacgc caccggtaaa ttagaggttg atgtagccac caatgtcgtc
     2341 aaaggcattg cagacggttg cgtgatttca ggttgtgctt tagtgggagg tgaaacggct
     2401 gaaatgccgg gaatgtacca cgaaggcgat tacgatttag ccggattctg tgtcggtgtg
     2461 gtagaaaaat ctaaaatcat tgacggttca acggtgaaaa ccggtgatgc cttaattgca
     2521 ttagcgtcca gtggcccgca ttccaacggc tattcgttaa ttcgtaaagt attggaagtc
     2581 agccgatcca atcctgcaac cgatttattg gaagacaaac ccttaagcga acacttatta
     2641 gccccaacta aaatttatgt aaaatcaatc ctaaaattag ttgaagaagt tgatgttcac
     2701 gctatcgctc acttaaccgg tggcggtttc tgggaaaata ttccacgcgt attgccggaa
     2761 tctgccaaag cggtgattga tgaatcgagc tggcaatggc cggcgatatt tagctggtta
     2821 caggaaaaag gcaatatcag ccgttatgaa atgtaccgta ctttcaactg cggtgtcgga
     2881 atggtgatag ccttacctga gagagatgtc gaaaaagccc ttactcttct taaagagagt
     2941 ggtgaaaatg cgtggcttat cggtaaaatt gaagacttag gcaccggtag cgagcaagtt
     3001 gaaatccgct aatttgcaaa ataaatgcta aaattgaccg cttgtagtaa ctgatctgca
     3061 aacggttaaa tttttattga ggaatgcaaa tgcaactatt ttggcctatc tactttgtct
     3121 taatgaacct agctgcctta tatttaatgt atagtgacaa acaaaaagcg atcaaaaaag
     3181 aatggcgagt gccagaaatt actctcctct ctttttgcct cttaggtggg gctatcggtg
     3241 tttacctagg tatgaaatac tttcgccata aaacccaaca ttggtatttc cacgtcgctg
     3301 ttgtgctttc tacttttgct tggctgattg gcttgcctta cttttattta gctttatcat
     3361 catattaatt tatcattact ttttttaat
//
LOCUS       DANLXF010000234         3377 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.234, whole genome shotgun sequence.
ACCESSION   DANLXF010000234 DANLXF010000000
VERSION     DANLXF010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.234"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..1919
                     /gene="cpdB"
                     /locus_tag="PXD66_002238"
     CDS             <1..1919
                     /gene="cpdB"
                     /locus_tag="PXD66_002238"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HDL6320487.1"
                     /translation="QPSAFAQTAKNKAGLRVIATTDIHGFLTDFDYYKDAPTEKFGFT
                     RTASLIKQARAEVKNSVLVDNGDLIQGNPISDYQSTKGAKEGKPEPSIMALNAMQYEV
                     GTLGNHEFNYGLSYLDNAIKQAKFPIVNANIVKPGTDEPFFTPYVIQQKEIVDEKGNK
                     HTIKIGYIGFVPPQVMIWDKANLEGKVEARDIVKTAQKYVPIVKKEGADIVVALAHTG
                     PSDEPYKEGAENCAFYLADVKGIDAVIFGHSHRLFPNKEFANSPNADIAKGTMKGVPE
                     SMAGYWGNNISVIDLSLAENDGKWGVVDGRAELRPIYNTEEKKATVENHPEIVALLKE
                     THEATRQFVSQPIGKATDNMYSYLALVQDDPTIQIVNQAQKAYVENVVKGLPELAGLP
                     ILSAGAPFKAGGRKNDPTGYTEVDKGELTFRNAADLYLYPNTLVVVKATGAELKEWLE
                     CSAGMFKQIDVASDKPQYLLDWTGFRTYNYDVIDGVSYQFDITQPARYDGECKLINPN
                     SHRVVNLTFDGKPVDPKQEFLIATNNYRAYGAKFPGTGDKHIVFAAPDENRQVLANYI
                     TTESKAHGQVSPKADNNWRIAPISSNAKLDIRFETSPTDKAKAFIKENAQYPMEYIEN
                     DETGFAVYRIDLTK"
     gene            complement(1992..>3377)
                     /locus_tag="PXD66_002239"
     CDS             complement(1992..>3377)
                     /locus_tag="PXD66_002239"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDL6320488.1"
                     /translation="EGSQFLYSTFTPRSNIYAPFVTRVVSFGFQAFILKYLIHYFNDN
                     FFSRSQHDVVSEYTQFISDTLNIEDSGEHIAQLHQLGYLPIRIKAIPEGKSVAVKVPL
                     MTIENTHSSFFWLTNYLETLINVSLWQPITSASIAFAYRKALAEFSEQTCDDNSHLPF
                     QAHDFSMRGMSSLESAESSGAGHLTSFLGTDTIPAISFVTAYYGSQHLIGTSIPASEH
                     SVMSSHGVDELPTFRYLMAKYPNNMLSIVADTTDFWHNITVNLPLLKQEILARPESAK
                     LVIRPDSGDFFTIICGNNSSPVEHERKGLIECLWDIFGGSINQKGYKVLNPHIGAIYG
                     DGVTYDKMIRILEGLTAKGFASSNIVFGVGAQTYQRNTRDTLGFAIKATSITINGVEK
                     AIFKAPKTDNGLKKSQKGRVKVISYDHYVDELTALDDFSDDLLEVVFENGKLVKSTNF
                     DEIRQNIAQQF"
BASE COUNT         1127 a          648 c          685 g          917 t
ORIGIN      
        1 cacaaccgtc tgcatttgca caaacagcca aaaataaagc tggcttgcgt gtcatcgcca
       61 ctacggatat tcacggtttc ttaactgatt ttgattatta caaagatgct ccaacagaaa
      121 agtttggttt tacccgtacg gcaagcctaa ttaaacaagc tagagcagaa gttaaaaaca
      181 gtgtgttagt cgataatggt gacttaattc aaggtaatcc tatttctgac taccagtcaa
      241 ccaaaggtgc aaaagagggc aaacctgaac cttcgattat ggcattaaat gcgatgcaat
      301 atgaagtcgg aacgctggga aatcatgaat ttaactacgg tttaagctat ttagataatg
      361 ctattaagca agcaaaattc cctattgtca atgcaaacat tgtgaagccg gggacagatg
      421 aaccattctt tacaccttat gttattcaac aaaaagaaat tgtagatgaa aaagggaata
      481 agcataccat taagattggt tatatcggtt ttgtaccacc acaagtgatg atttgggata
      541 aagctaatct tgaaggaaaa gttgaagctc gagatattgt gaagaccgca caaaaatatg
      601 tgcctatagt aaaaaaagaa ggggcagata ttgtcgttgc attggcacat accggccctt
      661 cagacgaacc ctataaagaa ggcgctgaga actgtgcgtt ttatctagct gatgtaaaag
      721 gtattgatgc agttattttc gggcattcac atcgcctgtt cccaaataaa gaatttgcaa
      781 attcaccaaa tgccgatatt gcaaaaggaa caatgaaagg cgtaccggaa agtatggctg
      841 gctactgggg aaataacatc agtgtgattg atttaagctt ggccgaaaac gatggtaaat
      901 ggggcgttgt ggacggtaga gcagagttgc gtccaattta taatacggaa gagaaaaaag
      961 caaccgttga gaaccaccct gaaatcgttg cattattaaa agaaacccat gaagcaaccc
     1021 gtcaatttgt gtctcaacct atcggtaaag caactgataa tatgtatagt taccttgcgt
     1081 tagttcaaga tgaccctacc attcaaattg tgaaccaagc tcaaaaagcg tatgtagaaa
     1141 atgtggtaaa aggcttgccg gagcttgcag gattaccaat tctaagtgcc ggagcaccgt
     1201 ttaaagccgg tggacgtaaa aatgacccga caggctatac cgaagtcgat aaaggcgaat
     1261 taactttccg caatgcggca gacttatacc tttacccaaa taccttagtg gtggttaaag
     1321 caacaggagc agaattaaaa gagtggttag agtgcagtgc aggaatgttc aaacaaattg
     1381 atgtcgcttc ggataaaccg caatatttac tggattggac aggtttccgc acctataact
     1441 acgatgtaat tgacggtgtc agctatcaat ttgatataac ccaaccggct cgttatgacg
     1501 gcgagtgcaa attgatcaac cctaattcgc accgtgtggt gaacttaacc tttgacggca
     1561 aaccggttga tcctaaacaa gaattcttaa ttgccaccaa caattaccgt gcttatggag
     1621 caaaattccc gggaacaggc gataaacata ttgtgttcgc cgcaccggac gaaaaccgcc
     1681 aagtgttagc gaattatatt accaccgaaa gtaaagctca cgggcaggta agtccgaaag
     1741 cggataacaa ctggcgtatt gcaccaatta gctcaaatgc aaaattggat attcgttttg
     1801 aaacctctcc aacagataaa gctaaagctt tcattaagga aaatgcacaa tatccgatgg
     1861 agtatatcga aaacgatgaa accggcttcg cagtttaccg tattgattta accaaataat
     1921 cagagctaaa tacaagcggt ctaatttgca aaaaaattaa caaatttgac cgcttgtttc
     1981 attccatttt tctaaaattg ttgtgctata ttttgccgaa tttcatcaaa attggtcgat
     2041 ttgaccaact taccattttc aaaaaccacc tctaaaagat catctgaaaa atcatccagt
     2101 gcggttagct cgtcaacata atgatcataa gagattactt ttactcgccc tttttgcgat
     2161 ttttttaagc cgttatcggt tttaggagct ttgaaaattg ccttttcgac accgttaatt
     2221 gtaattgaag tcgctttgat tgcaaaaccc agcgtatcac gagtattgcg ttgataagtt
     2281 tgagccccaa cgccaaacac aatattgctg gaagcaaaac cttttgccgt taagccctct
     2341 aaaatccgta tcattttatc ataagtgacg ccgtcaccat aaattgcccc aatatgcgga
     2401 ttaagcactt tataaccttt ttggttaata ctaccaccaa aaatatccca caaacactca
     2461 atcagacctt tgcgttcgtg ttcaacgggg gaggaattgt taccgcaaat aattgtaaaa
     2521 aaatcaccgc tatcagggcg gataaccaat tttgctgatt ccggtcgagc taaaatttct
     2581 tgttttaata atggcaaatt aacggtgatg ttatgccaga aatcggttgt atctgccaca
     2641 atagatagca tattatttgg atattttgcc attaaataac ggaaagtcgg cagctcatca
     2701 acaccgtgtg aactcatcac cgaatgttct gatgccggaa tagatgtacc gattaaatgt
     2761 tgagaaccat aataggctgt cacgaatgaa attgcaggaa tggtatctgt gcctaaaaat
     2821 gaggttagat gtccggcacc ggagctttct gctgattcta aggaactcat tccacgcata
     2881 gagaaatcgt gtgcctggaa gggtagatgg ctattatcat cacaggtttg ctctgaaaat
     2941 tcggctagag ccttacgata agcaaatgca atggaagctg atgtaatagg ctgccataat
     3001 gacacattaa tcagggtttc caaataatta gtcagccaaa aaaagcttga gtgagtgttt
     3061 tcaattgtca ttaaaggaac tttcactgca acagattttc cttctggaat agctttaatt
     3121 ctgataggta agtaaccgag ctgatgcaat tgtgcaatat gttcgccaga gtcctcaata
     3181 ttcaatgtgt cagaaataaa ttgagtatat tcagaaacaa cgtcatgttg tgagcgagaa
     3241 aaaaaattat cgttaaaata atgaatcaag tattttaaaa taaatgcctg aaagccaaat
     3301 gatacaacac gtgtaacaaa tggagcataa atattactac gaggtgtgaa tgtactatat
     3361 aaaaactgag aaccttc
//
LOCUS       DANLXF010000235         3368 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.235, whole genome shotgun sequence.
ACCESSION   DANLXF010000235 DANLXF010000000
VERSION     DANLXF010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.235"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            68..751
                     /locus_tag="PXD66_002240"
     CDS             68..751
                     /locus_tag="PXD66_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDL6320489.1"
                     /translation="MFGLFASILTGARGGWLAFIPICFFILFLNLRQLNKKAIFLILL
                     ALISSLSFFISKPEFGIQKRYNAAKNDIIVFLEKGNRHSSLGARFDMWENALIGIREK
                     PLLGHGSTGYETFKKKQIETKQMAKTTLRFNSLHNQYLEAFVKRGVVGFMALMAILII
                     PFVIFAKRLKAKNREVKCMAILGIVHIISHSFFFLSQSFLAHNSGSLFYFFVLILLYH
                     LVKQNEEII"
     gene            complement(853..1860)
                     /gene="rfbB"
                     /locus_tag="PXD66_002241"
     CDS             complement(853..1860)
                     /gene="rfbB"
                     /locus_tag="PXD66_002241"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDL6320490.1"
                     /translation="MIILITGGAGFIGSALVRYLIEHTPHTVINVDKLTYAGNLASLE
                     SVAHHERYHFEKADICASGQISEIFGKYQPDVVMHLAAESHVDRSISGAADFIQTNIV
                     GTYTLLEAARAYWDTLPEPRKSAFRFLHISTDEVYGDLAKSEELFTEKTPYNPSSPYS
                     ASKAASDHLARAWHRTYGLPTVITNCSNNYGAYQYPEKLIPLMILNALEGKSLPVYGN
                     GLQVRDWLFVEDHIRALYLVLQKGQIGETYNIGGHNEKTNIEVVRSICSILQEMVPNN
                     PLVVGQYADLITYVQDRPGHDFRYAIDTSKIEKELDWKPKETFESGLRKTVEWYLANR
                     N"
     gene            complement(1857..2438)
                     /locus_tag="PXD66_002242"
     CDS             complement(1857..2438)
                     /locus_tag="PXD66_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HDL6320491.1"
                     /translation="MLEKIEKLIDEINKLHLAFSKDYFETGKIEKVNLKHTFAKVPTP
                     HILAYRLNLHESINDYLYRADLEGISYFYRVKTSESILDKIERFKQRSEGYPVNSILN
                     DIFGARIIVSSEVIQQVMESLDYWQEKYGLKNWYLRDKDEYVGIHIYFKNSSNFYYPW
                     ELQIWDKKDAEKNIQSHIKYKRNFVNQISYSPL"
     gene            complement(2459..>3368)
                     /locus_tag="PXD66_002243"
     CDS             complement(2459..>3368)
                     /locus_tag="PXD66_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDL6320492.1"
                     /translation="FIGYFLSRSWIIPKIALVPFGRAINLNRIQNVEWIQLSEPILGE
                     KRINSIAVDLHYPDLSAEWHKFLAKCTLNGIPVYNVRQLEESLTGRVKIRHMYENDLG
                     SLLPSPIYSLVKRIIDIALIVATFPITLPIMTITAIAIRLESEGGAMFIQKRVGQGGK
                     EFNIYKFRSMCKDSEKDDAQFASSADMRVTRIGKFIRKTRIDELPQFFNVLKGDMSLI
                     GPRPEQTAFVDKFEEEIPFYNYRHIVKPGISGWAQVVQGYAADVDDTKVKVEHDFYYI
                     KHFSLWLDILIIFKTLKTMLTGFGAR"
BASE COUNT         1068 a          655 c          575 g         1070 t
ORIGIN      
        1 agcaattgcc ttttattggc tttcaaaaaa aagaattaaa actggcattg cctacctggt
       61 ttttgccatg tttgggttat ttgccagtat tctaacagga gcaagaggcg gctggcttgc
      121 attcattccc atttgctttt ttatcctatt tttaaattta agacagctaa ataagaaagc
      181 tattttcctg attctattag cacttatatc ttcactctca ttttttatct ccaaaccaga
      241 atttggcata caaaaacgat ataatgcagc taagaatgat attatcgtat ttttagaaaa
      301 aggaaataga cattcatctc tgggagctcg ttttgatatg tgggaaaatg cgcttatcgg
      361 aatccgtgaa aaaccgctat taggacacgg tagcacaggc tatgaaacat tcaaaaagaa
      421 acagattgaa acaaaacaaa tggcaaaaac aacgcttagg tttaatagct tacacaacca
      481 atacctagaa gcctttgtga aaagaggtgt agttggtttt atggcgctaa tggctatttt
      541 aattattcca tttgttattt ttgccaaacg attaaaggca aaaaatagag aggtaaaatg
      601 tatggcgata ttaggcattg tacatattat ttcgcattca ttctttttcc taagccaatc
      661 atttttagcc cataattcag gctcattatt ctatttcttt gtgttaattt tactctacca
      721 tttagtcaaa cagaacgaag agataatatg ataaaacaat agaagaaagt tgggaatata
      781 accgaggtgg taacttcatc ttctaccaca ggataaatgt cgggatattt cctctttttc
      841 tgcttcttac tactaattcc gattagccaa ataccattcc actgttttac gcaagccact
      901 ttcaaaagtt tctttcggtt tccagtcgag ttccttttcg atttttgaag tatcaattgc
      961 atagcggaaa tcgtggccag ggcgatcttg cacataagta attaaatcag catattgtcc
     1021 tacaacaagc gggttatttg gcaccatttc ttgcaaaata gaacagatag aacgaaccac
     1081 ttcgatattg gttttttcat tatgcccgcc aatattgtag gtttctccga tttgcccttt
     1141 ttgcaacacc aaatagaggg cacgaatatg atcttccaca aacagccaat ctctgacctg
     1201 caagccgttg ccgtaaacag gtagggattt accttctaag gcatttaaaa tcattaacgg
     1261 aataagtttt tcgggatatt ggtatgcccc ataattattg gaacagttgg tgatgacagt
     1321 cggtaaaccg taggtgcgat gccaagcccg agccagatga tcgctggcag ctttagatgc
     1381 cgaatagggg ctacttggat tataaggggt tttctcggta aataattcct cgcttttggc
     1441 taaatcacca tagacctcgt cggtcgagat atgtagaaag cgaaacgccg atttccttgg
     1501 ctcaggtaac gtgtcccaat aggctctcgc tgcctctaat aaagtataag tgccgacaat
     1561 attggtttga ataaaatctg ctgccccaga aatagagcga tcaacatggc tttccgctgc
     1621 taagtgcatt accacatcag gctgatattt gccaaaaatc tcagaaattt gaccgcttgc
     1681 acaaatatct gctttttcaa aatggtagcg ttcgtgatgt gcgacactct ccaatgaggc
     1741 taaatttccc gcataggtga gcttatccac attgataact gtgtgtggag tatgttcaat
     1801 cagatagcga actaatgcag atccgataaa accggctccg ccagtgatga ggatgatcat
     1861 aaaggtgaat atgaaatttg attcacgaaa tttcgtttgt atttaatatg gctttgaata
     1921 ttcttttcag cgtctttttt atcccaaatt tgcaattccc aaggataata aaagttactt
     1981 gaattcttaa aataaatatg aatacccaca tattcatctt tatctctcaa ataccaattt
     2041 tttagtccat atttttcttg ccaataatct aatgactcca ttacttgctg aatcacttca
     2101 ctagaaacaa taatacgagc tccaaaaata tcgttgagaa tgctatttac tggataacct
     2161 tcactacgct gtttaaaacg ctcgatttta tctaaaatcg attctgaggt ttttactcgg
     2221 taaaaataag aaattccctc taaatctgca cgatataaat aatcattaat agattcgtgt
     2281 aaattaagac gataagctaa aatatgtggc gtaggcactt tggcaaaagt atgcttaaga
     2341 ttcacttttt caatcttacc tgtttcaaag tagtctttcg aaaaagctaa gtgcaacttg
     2401 ttgatttcat caatgagttt ttctattttc tcaagcatgc tattcactct ctaatttatt
     2461 atcttgcccc aaatccagtt agcattgttt tcaatgtttt aaaaataatt aaaatatcaa
     2521 gccataaaga aaaatgctta atatagtaga agtcatgttc tacttttact tttgtatcat
     2581 caacatctgc cgcataacct tggacgacct gagcccaacc ggaaataccc ggtttaacga
     2641 tatggcgata attatagaat ggaatttcct cttcaaattt atctacaaag gctgtctgtt
     2701 ctgggcgagg accaattaag ctcatatcac cttttaatac attaaaaaac tgaggtagct
     2761 catcaatacg agttttacgg ataaatttcc caatacgagt taccctcata tccgcactcg
     2821 aagcaaactg tgcatcatct ttttcggagt ctttacacat actacgaaat ttataaatat
     2881 taaactcctt tccaccttgc ccaacgcgtt tttgaataaa cattgctcca ccttcacttt
     2941 ctaagcgaat agcaatagcg gtgattgtca taataggtaa agtaatggga aacgtagcaa
     3001 caatcaatgc aatatcaatg atacgcttca ctaatgagta aattggagaa ggaagtaatg
     3061 aacctaagtc attttcatac atatggcgaa ttttgacacg cccggttaaa gattcctcaa
     3121 gctggcgaac gttatataca ggaataccat tcaatgtaca tttcgctaaa aatttatgcc
     3181 attctgcgga caaatcagga tagtgtaaat ctaccgcaat agagttaatt ctcttttccc
     3241 ccaatattgg ttcacttaat tgaatccact ctacattctg aattcggttc aaattgatag
     3301 ctcttccgaa tggaaccaaa gcgattttag ggataatcca acttcttgaa agaaaataac
     3361 caataaaa
//
LOCUS       DANLXF010000236         3362 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.236, whole genome shotgun sequence.
ACCESSION   DANLXF010000236 DANLXF010000000
VERSION     DANLXF010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.236"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            97..1272
                     /locus_tag="PXD66_002244"
     CDS             97..1272
                     /locus_tag="PXD66_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012072290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDL6320493.1"
                     /translation="MSVIKMTDLDLAGKRLFIRADLNVPVKDGKVTSDARIVATIPTL
                     KLALQKGAKVMVTSHLGRPTEGVFEEENSLQPVVDYLNASDLGVPVRLVRDYLDGVEV
                     NENEIVVLENVRINKGEKKNDPELAKKYASLCDVFVMDAFGTAHRAEGSTYGVAEYAP
                     VACAGPLLAAELDALGKALKEPQRPMLAIVGGSKVSTKLTVLDSLSKIADQLIVGGGI
                     ANTFIAAEGHAVGKSLYEEDLIPEAKRLAQVTNIPVPVDVRVGTEFSETAPATEKAVS
                     EVRADESIFDIGEKSSEQLAEIIRNSKTILWNGPVGVFEFPNFRKGTEVISNAIAEAT
                     ANGAFSIAGGGDTLAAIDLFGIKDKISYISTGGGAFLEFVEGKVLPAVEILEKRANG"
     gene            1369..2445
                     /gene="fbaA"
                     /locus_tag="PXD66_002245"
     CDS             1369..2445
                     /gene="fbaA"
                     /locus_tag="PXD66_002245"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HDL6320494.1"
                     /translation="MSLLNIVKPGVVTGDDVQKVFAYAKEHNFAIPAVNCVGSDSVNA
                     VLETAARVKSPVIVQFSNGGAQFYAGKGIKPASGARADVLGAIAGAKHVHQLAEEYGV
                     PVILHTDHAAKNLLPWIDGLLEAGEKHFAETGRPLFSSHMIDLSEEPIEENMEICREY
                     LARMDKIGMTLEIEIGVTGGEEDGVDNSDVDESKLYTQPSEVLYVYDQLSPISPRFTI
                     AAAFGNVHGVYKPGNVKLKPSILGASQEYVSKERGLPAKSLDFVFHGGSGSSREEIRE
                     AISYGAIKMNIDTDTQWAAWDGILQFYKANEAYLQGQLGNPTGPDAPNKKYYDPRVWL
                     RKMEESMSKRLEQSFEDLNCVNVL"
     gene            2546..3340
                     /locus_tag="PXD66_002246"
     CDS             2546..3340
                     /locus_tag="PXD66_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL6320495.1"
                     /translation="MALLEVNHLNKRFVERSSLFHKQDFYAVKEVSFTLNRKETLAII
                     GENGAGKSTLAKMVAGMVGPTSGELIFRGTPLSFGDYKYRAKHIRMMFQDPNDAFDPN
                     YNIGQILDAPLRLATDLNEEERNERIFNTLKLVGMYPEQALVPISEASSGQKQRIAFA
                     RALILEPEIIIADDTINSLDFSVKTQLINLMLSVQQRQGIAYIYVGQNLGLIKHIADK
                     IMVMQSGEVVEYGLTKEVLLNPQHQITKRLIESHFGKHLSSYAWAS"
BASE COUNT         1035 a          613 c          737 g          977 t
ORIGIN      
        1 aatcatttat ttgatctagg ttagcccaaa atgattagaa actgatataa tccgaccgtt
       61 atcactttta aattttaatc taaagaggaa caaactatgt ctgttattaa aatgactgac
      121 cttgatttag cgggtaaacg tttatttatt cgtgctgacc ttaacgtacc ggtaaaagat
      181 ggcaaagtaa catcagatgc ccgtattgtt gctaccattc caaccttaaa attagcgtta
      241 caaaaaggtg ctaaagtaat ggtaacttcg catttaggtc gtccgacaga aggcgtattt
      301 gaagaggaaa actctttaca acctgtagtt gattacttaa atgcatcaga tttaggtgtt
      361 cctgttcgtt tagtgcgtga ttacttagac ggtgttgaag taaacgagaa tgaaatcgtg
      421 gtgcttgaaa acgtgcgtat taacaaaggt gagaagaaaa acgaccctga gttagcaaaa
      481 aaatatgcat cactttgtga tgtgtttgta atggacgcat tcggtacagc tcaccgtgcg
      541 gaaggttcaa cttacggtgt ggcagaatat gcccctgttg cgtgtgcagg tccgttatta
      601 gccgctgaat tagatgcatt aggcaaagca ttaaaagagc cacaacgtcc aatgttagcg
      661 attgtgggtg gttctaaagt atctactaaa ttaactgtgc ttgattcgct ttcaaaaatt
      721 gccgaccaat taatcgtggg cggtggtatt gccaatacct ttattgccgc agaaggtcac
      781 gctgtgggta aatcattata cgaagaagat ttaatccctg aagcaaaacg tttagcacaa
      841 gtaaccaata ttcctgttcc ggttgatgtg cgtgtaggta ctgaattctc tgaaactgca
      901 ccggcaacgg aaaaagcggt gagtgaagta cgagctgatg aatcaatttt cgatattggt
      961 gaaaaatcgt cagagcaatt agctgaaatt atccgtaatt caaaaaccat cctttggaat
     1021 ggtccggtcg gcgtgtttga gttccctaac ttccgtaaag gaacagaagt gatttcaaat
     1081 gcgattgcag aagcaacggc aaacggtgca ttctcaattg ccggtggtgg cgatacctta
     1141 gctgcgattg atttattcgg tatcaaagac aaaatttctt acatttcaac cggtggcggt
     1201 gcgttcttag aattcgtgga aggtaaagta ttaccggcag tggaaatctt agaaaaacga
     1261 gctaacggct aacagttaca agcggtcaaa ttttgtgaaa attttgcaag atttggccgt
     1321 atattttaac aatgtgcttt tgcacatttc atacaaagga agaaaaaaat gtcattatta
     1381 aacatcgtaa aaccgggtgt agtaacaggc gacgacgtac aaaaagtttt cgcttatgct
     1441 aaagaacaca acttcgctat tccggcagta aactgcgtgg gttcagattc agttaatgcc
     1501 gtattagaaa cagccgctcg tgtaaaatca ccggtaatcg tgcaattctc aaatggtggt
     1561 gcgcagttct atgcgggtaa aggcattaag ccggcttcag gcgctcgtgc agatgtatta
     1621 ggggcgattg cgggtgcaaa acacgttcat caattagcag aagaatatgg tgtgcctgtg
     1681 attcttcaca ccgaccacgc tgcgaaaaac ttattacctt ggatcgatgg cttacttgaa
     1741 gcaggcgaaa aacacttcgc agaaaccggt cgtccgttat tctcttcaca tatgatcgat
     1801 ttatctgaag agccaatcga agaaaatatg gaaatctgcc gtgaatattt agcccgtatg
     1861 gacaaaatcg gtatgaccct tgaaatcgaa atcggcgtaa ccggtggtga agaagatggc
     1921 gtagataatt ctgatgtaga tgaatctaaa ctttacaccc aaccttctga agttttatat
     1981 gtttacgacc aattaagccc aatcagccca cgtttcacga ttgcggcagc atttggtaac
     2041 gtacacggtg tttacaagcc gggtaatgta aaattaaaac catcaatctt aggtgcttca
     2101 caagagtatg tatctaaaga gcgtggttta ccggcaaagt cattagactt cgttttccac
     2161 ggcggttctg gttcttcacg tgaagagatt cgtgaagcaa tcagctacgg tgcaatcaaa
     2221 atgaacattg acactgacac ccaatgggct gcgtgggacg gtattcttca attctacaaa
     2281 gcaaacgaag catatttaca aggtcaatta ggtaacccga caggccctga tgcaccaaac
     2341 aaaaaatact acgatccacg tgtttggtta cgtaaaatgg aagagtctat gtctaaacgc
     2401 ttagagcaat ctttcgaaga cttaaactgt gtgaacgttt tataatttgc aaaaaattcc
     2461 ataaatcagg ccgcttgtat aagcggtctt tttttattta ttttttgcgg taaactatca
     2521 ctttatttcc aagcaccaat ttattatggc attacttgaa gttaatcatc tgaataagcg
     2581 ttttgttgaa cgtagtagcc tatttcataa acaagatttt tatgcggtaa aagaagtttc
     2641 ttttacccta aatcgcaagg aaactttagc gattattggt gaaaacggag ctgggaaatc
     2701 taccttggct aaaatggttg ctggtatggt tgggccaaca tctggagaac tgatttttag
     2761 aggaacaccg ctttcatttg gcgattataa ataccgagct aagcatattc gtatgatgtt
     2821 tcaggaccct aacgatgcct ttgatcctaa ttataatata ggacaaattt tagatgctcc
     2881 gctccgcttg gcgacagatt taaatgaaga ggaaagaaat gagcgtattt ttaatacgct
     2941 aaaattggta ggaatgtatc ctgagcaagc gctcgttcct atttcagagg cttccagtgg
     3001 gcaaaagcag cgtattgcgt ttgctcgtgc tttaatttta gaaccggaaa ttattattgc
     3061 ggatgatacg attaattcac tggatttttc agtcaaaaca caattaatta atttaatgtt
     3121 aagtgtccag cagcgacaag ggattgctta tatttatgtg gggcaaaatt taggtctgat
     3181 caaacatatt gccgataaaa taatggtgat gcaaagtggc gaagtagtag agtatggctt
     3241 gacgaaagag gttttactga atccgcaaca tcaaattacg aaacgtttaa ttgaaagtca
     3301 ttttggaaag caccttagtt cctatgcttg ggcaagctaa atctttactc ctgctctaat
     3361 at
//
LOCUS       DANLXF010000237         3326 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.237, whole genome shotgun sequence.
ACCESSION   DANLXF010000237 DANLXF010000000
VERSION     DANLXF010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.237"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            58..846
                     /locus_tag="PXD66_002247"
     CDS             58..846
                     /locus_tag="PXD66_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320496.1"
                     /translation="MFLSHFKQVLNANIEADGKDWTIIDVFGGSGLLAHTAKREKPLA
                     RVIYNDFDNYAERLNHIKETNQLRQEIYQIVDEIIPKNKRISNEIKAKIINKINDFEG
                     FKDLKCLSSWLLFSGEQVATLDELFKHDFWHCVRQSDYPEATGYLDDIEVVSESFHQL
                     LPRFHDKEKVLLILDPPYLCTRQESYKQENYFDLIDFLRLIDLTKPPYIFFSSTKSEF
                     IRFIHYMVQNRKENWQAFEGAERIVVNASASYNGKYEDNMVYKF"
     gene            complement(883..3321)
                     /locus_tag="PXD66_002248"
     CDS             complement(883..3321)
                     /locus_tag="PXD66_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family igA-specific metalloendopeptidase"
                     /protein_id="HDL6320497.1"
                     /translation="MKHTFKLSLLATSIAISLSPTLLASIVRGDVDYQYFRDLAENKG
                     KFFVGATNIPVIDKTGKNIGTFLQVTPTKQVEVESNISPKPNVSLSECFDNESLPGCY
                     EFLYGGGDEDSNDLPVFETVTGETMSIPMIDFSSITRQTGIATLVNPQYVISVNHNQG
                     YRDIQFGDSSSEKADDHHYNYSVVRRNNYMPDPNKERTDLGKGIEKRERNDEGEKLIL
                     DGSGNPTPRWDYHAPRLSKLVTEVAPANEIERTTENVQDFYSVFSDPFIFPMFIRAGS
                     GRQAVVNNERDRLNNGRIEVGNGPFLTGGSVLPVTNGDPSVPGSDFNSRILVAKTENN
                     TINDVFKDHGYGPLTTLGLPGDSGSALFGYDVRTKKWVVLGVYSDYFSENNTLGGDVY
                     KSYWNYHHPHYVRALEKENNAGAINANGARLTWTPSGNTSSIVGGNAPLTVNLADTSL
                     PTLQGHNGNTGDNPWNPQLQHGKTFHILGENNTLSLTENINQGAGAIHFHGNTTVEGM
                     KAGITWLGAGVDIDKDKNVIWKISNPAGDRLSKIGQGTLTINGKGENKGSVSVGDGTV
                     ILNQQADENNKKSAFSELGIVSGRPTVILNSSDQMDPNNIYFGYRGGRLDVNGNDLTF
                     RRIQNSDEGAMVVNHNTAQTANITLTGIVSPTIDQVKVQAITSPDQVSRHLDKDIFTY
                     QRFPHLPTNFIRLKKPLSVAEITAIFNARKVFIDLDFATGLIRENEYFEVLKVTDESA
                     AKQQILAEKIKAAEGTVQGFNGYFGETDPSKTNGRLNITMNSNCSEPTKLRNATTKTT
                     KENRSFTFLILMVF"
BASE COUNT          983 a          621 c          658 g         1064 t
ORIGIN      
        1 tgtgagatag agaaagtatt taaacaagct cctttaccct tttcagggca aaaacggatg
       61 tttttatccc atttcaaaca ggtgttaaat gcaaatattg aagcagacgg gaaagattgg
      121 actattatag atgtttttgg tgggagtggg ttgcttgcac ataccgcaaa gcgtgagaaa
      181 ccgttagcaa gagtgattta taatgatttt gataattacg ccgaacggct aaaccatatc
      241 aaagagacaa atcaacttcg ccaagaaatc taccaaattg ttgatgaaat tataccaaaa
      301 aataagagaa ttagcaatga aattaaagct aaaatcatca ataaaatcaa tgattttgag
      361 gggtttaaag acctcaaatg cttaagttct tggttattgt ttagcggtga gcaagttgca
      421 actttagatg agttatttaa gcacgatttt tggcactgtg tgcggcaaag tgactatcct
      481 gaagcaacgg gttatcttga tgatattgag gtggttagtg agtcctttca tcaattattg
      541 ccgagatttc acgacaaaga gaaagtatta ttgattcttg acccacctta tctttgcaca
      601 agacaggaaa gctataagca ggaaaattac tttgatttaa ttgatttctt acggttgata
      661 gaccttacta aaccacctta tatcttcttt agctcaacca aaagcgagtt tattcgcttt
      721 attcactata tggttcaaaa cagaaaagaa aactggcaag cgtttgaggg agcggaaaga
      781 atagtggtta atgcctcggc aagttataac ggcaaatatg aggataatat ggtgtataag
      841 ttctaaaaat ttaaacgccc tttgatagtg atttaaaggg cgttaaaaaa ccattaaaat
      901 taagaaagta aagctcctgt tttctttcgt agttttcgtg gttgcgtttc gcagttttgt
      961 tggctcgcta caattactgt tcattgtaat gtttaatcga ccattggttt tgctaggatc
     1021 agtttcacca aaataaccgt taaaaccttg gacggtgcct tctgctgctt tgattttttc
     1081 agcaagtatt tgttgctttg cagcactttc atctgttacc ttgagtactt caaagtattc
     1141 gttttcacga atgagaccag tagcaaagtc taaatcgatg aaaactttac gcgcattaaa
     1201 aatagctgtg atctctgcaa cgcttaaagg ttttttcaaa cggataaaat tagttggcaa
     1261 gtgtggaaaa cgttgatatg tgaaaatatc tttgtccaaa tgacgagaaa cctgatctgg
     1321 actagtaatt gcttgaactt tcacttggtc aattgttgga ctaactatgc cggttagtgt
     1381 aatgtttgct gtttgggctg tattatgatt gacaaccatc gctccttcat cgctattctg
     1441 aatacggcgg aacgtgagat cattgccgtt gacatctaag cgcccaccac gataaccaaa
     1501 atagatattg ttaggatcca tttgatctga tgaattgagg attacggtag gtctgccact
     1561 cacaatacct agctctgaaa atgcagattt tttgttattt tcatccgctt gttgatttaa
     1621 tataacagtg ccgtcaccaa cgctaacgga gcctttgttt tctcctttgc cgttgatagt
     1681 tagtgtccct tgtccgattt ttgataatct gtcaccagca gggttactga ttttccagat
     1741 cacattttta tctttatcaa tatcaacacc ggctcctaac caagtaatgc ctgctttcat
     1801 accttccaca gtggtattac catgaaaatg gattgctcct gcaccttggt tgatattctc
     1861 tgtgagactt agcgtattgt tttcacccaa aatatggaat gttttaccgt gttggagttg
     1921 aggattccaa gggttatcgc ctgtattacc attatgacct tgaagtgttg gtaatgaagt
     1981 atctgctaaa ttaactgtaa gaggagcatt ccctcctacg attgagctgg tattgccaga
     2041 aggtgtccaa gtgagtctag caccatttgc attaatagcg cctgcattat tttctttttc
     2101 taaagcacgt acataatgtg ggtggtgata gttccagtaa gatttataaa catctccccc
     2161 aagcgtatta ttctctgaga aatagtcact atatacacct aacacaaccc attttttggt
     2221 acgaacatca taaccaaaaa gagcggagcc actatcgcca ggtaaaccga gagttgttaa
     2281 gggaccgtag ccgtggtctt taaatacatc gtttatggta ttattttcag tttttgctac
     2341 taaaatacgg ctgttaaaat cgctgcctgg aacgcttgga tcgccattag taacaggcag
     2401 aacgctacct cctgtcaaaa aaggaccgtt accgacttca atacgcccgt tgtttaagcg
     2461 atctctttcg ttgttaacca ctgcttgtct accagaaccg gcacgaataa acatggggaa
     2521 aataaaaggg tcgctaaata cactataaaa atcttgtaca ttttcggtag tgcgttcaat
     2581 ctcattagct ggagcgactt cagttactaa tttcgataac ctaggtgcgt ggtagtccca
     2641 gcggggcgtt gggttacctg aaccatctaa aatcagtttt tctccttcgt cattacgttc
     2701 acgtttttct atgcctttcc cgagatctgt tctttcttta tttggatcag gcatatagtt
     2761 attacggcgc accacactat aattgtaatg atggtcgtcg gctttttcgc tagatgaatc
     2821 accaaattga atgtcccgat agccttggtt gtggtttaca ctaataacgt attgtggatt
     2881 aactaatgtt gcaatccctg tttgacgagt aatggaagag aagtcaatca ttggaataga
     2941 catagtctca cctgttactg tttcaaaaac aggtagatca ttagagtctt catcacctcc
     3001 accatagaga aattcataac aaccgggtaa actttcatta tcaaagcatt cagataggct
     3061 tacattcggc ttaggtgaaa tatttgattc cacttcaacc tgcttagttg gtgtaacctg
     3121 taaaaaagta ccgatgtttt tgcccgtttt atcaataact ggaatattgg ttgccccaac
     3181 aaaaaatttg cctttatttt ctgctaaatc acgaaaatat tgataatcga catcaccacg
     3241 tacaatagat gccagtaatg ttggtgaaag agaaatagca attgatgttg ctagaagaga
     3301 gagcttgaaa gtatgcttca ttatta
//
LOCUS       DANLXF010000238         3309 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.238, whole genome shotgun sequence.
ACCESSION   DANLXF010000238 DANLXF010000000
VERSION     DANLXF010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.238"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            163..384
                     /locus_tag="PXD66_002249"
     CDS             163..384
                     /locus_tag="PXD66_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HDL6320498.1"
                     /translation="MNIYTDFLSREEIEFITQCKQKNLIKKQLNIMGIPFKENANGFP
                     VVRRDYAQAKQRKPIATNDSDWVSNAFKA"
     gene            406..1446
                     /locus_tag="PXD66_002250"
     CDS             406..1446
                     /locus_tag="PXD66_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL6320499.1"
                     /translation="MARPRKYENNGLPQNLLCRRRKRANGSIVEYYFYVLADGKERSL
                     GTNKHEAILEAAKLNFENSRKSPIVLFIDVAKRYELEVVPTKKAKNTRQSNLQAIHWL
                     CKFFGNPPAPLEKIEPKHISQYLQWRKDTPAVANIEVGLFNTIWNMAREWGYTALPSP
                     SQGVKKFPTKYREVYVEDYILDKIYEFADERMADIIETAYLLGQRPIDICNIHRSHIY
                     DGILHITQQKTGKKVRFEISGRLKEILDKRLGYESDWIFTNKWGKKLKRRSLGDHFKE
                     IRERAMKAYPELADEIAKVQMRDMRAKAATDISLMTTDEQAQKQLGHTSKRMTHHYIR
                     KDKILKPTDEIT"
     gene            complement(1605..1892)
                     /gene="rplY"
                     /locus_tag="PXD66_002251"
     CDS             complement(1605..1892)
                     /gene="rplY"
                     /locus_tag="PXD66_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HDL6320500.1"
                     /translation="MSFKFEAEVRSAQGKGASRRLRHNGQVPAIIYGGNAEPVSIILD
                     HDKVNNAQVHDSFYNEVLTIVVAGKEEQVKVQAIQRHPTKPKLVHLDFKRA"
     gene            complement(2044..>3309)
                     /locus_tag="PXD66_002252"
     CDS             complement(2044..>3309)
                     /locus_tag="PXD66_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme"
                     /protein_id="HDL6320501.1"
                     /translation="SDLKQQALDFHEFPIPGKISVTPTKALDTQHDLALAYSPGVAEP
                     CLAIEKDPSASYRYTARGNLVAVISNGTAVLGLGNIGALASKPVMEGKGVLFKKFAGI
                     DVFDIEINETDPEKLVEIIASLKPTFGGINLEDIKAPECFYVEQKLRERMGIPVFHDD
                     QHGTAIITGAAVINGLQVVGKAISEVRLVVNGAGASAIACTNLLRSLGVKKENITMCD
                     SKGVIFQGRGDKMDETKQFYAIEDNGWRTLADAVANADVFLGCSKGGALTQEMVKTMA
                     KDLLILALANPVPEITPAEAKAVRPDAIVCTGRSDFKNQVNNVLCFPFLFRGALDVGA
                     KTINEEMKMAAAHAIAELAKEPVPFEVISTYGELSFGPEYIIPTPFDPRLIVNVSIGV
                     AKAAIQSGVATRPITDWEAYAEQLKKLVA"
BASE COUNT         1105 a          686 c          604 g          914 t
ORIGIN      
        1 atcaaataag atgccccaaa acctatgcta agttgagaga aatcctagaa gccaacgaag
       61 aaaatgttgt ccgcatcggt aatgtgagca tttcgatagg ttcaacgact taacgattat
      121 ttaaccaaat ccctctaaat gaggggttta tttggagaga atatgaacat ctacactgac
      181 ttcctttctc gtgaagagat tgaatttatt actcaatgca agcagaaaaa tctaatcaaa
      241 aaacaactca acattatggg cattccattt aaagaaaatg caaatggatt ccctgttgtc
      301 cgccgtgatt atgctcaggc taaacagcgt aaacctattg caacgaatga ttctgactgg
      361 gtatcaaatg catttaaagc gtaaattgag aagaaggagg cacttatggc acgccctcga
      421 aaatatgaaa acaacggatt gccacaaaac ctactctgcc gccgcagaaa acgagcaaac
      481 ggaagtatag ttgaatacta cttttacgtt cttgccgacg gcaaagaaag gtctctcggt
      541 acaaacaaac acgaagcaat tttagaagca gccaagctaa attttgaaaa tagtagaaaa
      601 tccccaattg ttttatttat tgatgtggca aaacgctatg aattggaagt tgttccaacc
      661 aaaaaagcca aaaacactcg tcaatctaat ttacaggcta ttcattggct atgtaaattc
      721 tttggtaacc cacctgcacc gctcgaaaaa attgagccaa agcacatcag tcaatattta
      781 caatggagaa aagacacgcc ggctgttgct aatattgagg taggtttatt taacacaatt
      841 tggaatatgg caagagaatg gggatacact gctcttccaa gcccatcaca aggtgtaaaa
      901 aaattcccga ccaaatatcg tgaagtttat gtagaggatt atatccttga taaaatctat
      961 gaatttgcag atgaaagaat ggcggatatt atcgaaacag cctatttgct gggacaaaga
     1021 ccgattgata tttgtaatat acaccgctca cacatctatg acggtatatt gcatattact
     1081 caacagaaga cagggaaaaa agtgagattt gaaattagtg gtcggctaaa agaaatttta
     1141 gataaaaggt tgggatacga atcagactgg atttttacca ataaatgggg gaaaaaatta
     1201 aaacggcgaa gtttaggcga tcattttaaa gaaataaggg agagagcaat gaaagcatat
     1261 cctgaacttg ctgatgaaat cgctaaagta caaatgcgtg atatgcgtgc caaagcagcc
     1321 accgatattt ccctaatgac aaccgatgaa caagctcaaa aacaacttgg gcatacatca
     1381 aaaagaatga cacatcatta catcagaaaa gacaaaatcc tcaaacctac cgatgaaatc
     1441 acctaaaaat aaagcccttt tgccaaatac aaaagagcca tttactcacc taaaatccgt
     1501 tccgaaacta ttttgtaaat aattgatttt attgacctaa aaaactataa aatcgcaaaa
     1561 agtttcggaa cagaaaacag cgttaaaccc ttgtaaatca aggattaagc acgtttgaag
     1621 tctaagtgaa ctaattttgg tttagttggg tggcgttgaa ttgcttgtac ttttacttgc
     1681 tcttctttac ctgcaacaac gatagttaat acttcgttat agaatgaatc gtgaacttgt
     1741 gcgttgttca ctttatcgtg atctaaaatg attgatacag gctctgcatt accaccgtag
     1801 atgattgcag gaacttgacc attgtgacgc aggcggcggc tcgcaccctt accttgcgca
     1861 gaacgaactt cagcttcaaa tttgaatgac atagttttat tctcttaaat aaaatgggga
     1921 aatccccgtg aatagaaaaa acagcaggcg acccagctgt ttccgaaaaa atatcggcat
     1981 atccgtctcg atatgccaac tacgtatttt actgaagttg cagaaaaatg caatgtaaaa
     2041 atattaagca accagttttt tcaactgctc tgcataagct tcccaatctg taattggtcg
     2101 agttgctaca cctgattgaa ttgctgcttt tgctacacca atcgagacat tcacgatcaa
     2161 acgtggatca aatggcgttg gaataatata ttccgggcca aacgataatt cgccataagt
     2221 tgaaataact tcaaacggca caggttcttt agccaactct gcaatcgcat gggctgctgc
     2281 cattttcatt tcttcattaa tggtttttgc cccaacatct aatgctccac ggaataagaa
     2341 cgggaaacaa agcacattat tcacttggtt tttaaagtct gaacgaccgg tacatacaat
     2401 cgcatctgga cggacagcct tcgcttcagc aggtgtaatt tccggcacag ggttcgctaa
     2461 agcaagaatt aatagatctt ttgccattgt tttaaccatt tcttgcgtta aagctccgcc
     2521 ttttgaacaa cctaagaaaa catctgcatt cgcaacggca tctgccaatg ttctccaacc
     2581 attatcttct attgcataga attgtttggt ttcatccatc ttatcaccac gaccttggaa
     2641 aataacgccc ttagaatcgc acattgtgat attttctttt ttcacaccta atgaacggag
     2701 taagttagta cacgcaatcg ctgatgctcc agcaccgttt accactaaac gtacttctga
     2761 aattgcttta cctaccactt gtaatccgtt aatcaccgcc gcaccggtaa taattgccgt
     2821 accgtgttga tcatcgtgga acacagggat tcccatacgt tcacgtaatt tttgctctac
     2881 ataaaaacat tcaggtgctt taatatcttc cagattaata ccaccaaaag tcggttttaa
     2941 cgatgcaata atctccacta atttctccgg atcagtttca ttaatttcaa tatcaaacac
     3001 atcaattccg gcaaatttct taaatagaac acctttgcct tccatcacag gcttacttgc
     3061 taaagcacca atattgccta agcctaacac tgctgtacca ttagaaatta ctgcaactaa
     3121 gttacctcgt gccgtataac gataagaagc tgaaggatct ttttcaatcg ctaagcaagg
     3181 ctcagcaacc ccaggagagt acgctaaagc taaatcgtgc tgagtatcta atgctttagt
     3241 aggagtaacg gaaatttttc cggggattgg gaactcgtga aaatcaagcg cctgttgttt
     3301 taaatcgct
//
LOCUS       DANLXF010000239         3268 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.239, whole genome shotgun sequence.
ACCESSION   DANLXF010000239 DANLXF010000000
VERSION     DANLXF010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.239"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(7..579)
                     /gene="pdxT"
                     /locus_tag="PXD66_002253"
     CDS             complement(7..579)
                     /gene="pdxT"
                     /locus_tag="PXD66_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HDL6320502.1"
                     /translation="MKIAILALQGAFSEHTDKLQQLGVESMEIRQLADLNQDFDGLIL
                     PGGESTVQGKLLRELGLLEPLRQKILNGLPTLGTCAGLILLAENLVNDDKKHLATLPV
                     TVKRNAYGRQLGSFLVEEEVKYLGKIPLTFIRAPYIESTGKDVEILAKISGKIVGVKY
                     QNQIGISFHPEVNNDLSFHRYFVEMCRKNK"
     gene            complement(579..1451)
                     /gene="pdxS"
                     /locus_tag="PXD66_002254"
     CDS             complement(579..1451)
                     /gene="pdxS"
                     /locus_tag="PXD66_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HDL6320503.1"
                     /translation="MTNRYDLNRQLAQMLKGGVIMDVTTPEQACIAEEAGACAVMALE
                     RIPADIRAAGGVSRMSDPKMIKSIQAAVSIPVMAKVRIGHVTEAQILQAIEIDYIDES
                     EVLSPADDTFHINKRKFEVPFVCGAKNLGEALRRINEGAAMIRSKGEPGTGNVVQAVH
                     HLRKMKQEIARLVSHTTDELYHVAKELQVPFDLVQYVHQYQKLPVVTFAAGGVATPAD
                     AALMMQLGAEGVFVGSGIFKSGDPAKRARAIVQAVTNYNDPKLLAELSEDLGEAMVGI
                     NEQEIELLMAARGV"
     gene            1662..3065
                     /locus_tag="PXD66_002255"
     CDS             1662..3065
                     /locus_tag="PXD66_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDL6320504.1"
                     /translation="MLTYTFNTQEGTALYEQLYRFLKNDIESNILIAGDKLPSKRAFA
                     KHLGISVMTVETAYQQLVAEGYVNTQPKKGFFVNALNLPEKKEAISVQLQKISSEQYE
                     KNKKWQADLTNRQTLAENFPFSVWTKLVREVLKTHQSELMERAESGGVWTLKFAIANH
                     LRAFRGMIVNPTQIIIGAGTEYLYGLLIQLLGLDKHYALTEPSYDKLRRIYHSYRLST
                     ATVSMPFSIDELNSKNVDVIHTSPSHHFPTGHVMPIAKRYELLAWVAQKAERYIVEDD
                     YDSEFRFVGQPIPALKSIDMLGRVIYMNTFSKTLSSTVRIAYMVLPPELLNRFQQELG
                     FYASTVSNFEQYILAEFIQQGYFEKHINRMRAYYQKKRDKLLKAFKQGNLAEKIAIKE
                     EHSGLHFIVQLNTTLTERQILQAAEEQGIKFVALSAYYQDKSQIQQNAFIIGYSNLKD
                     DQINYVAESLKKILDNE"
BASE COUNT         1009 a          651 c          598 g         1010 t
ORIGIN      
        1 tttcttttac ttattttttc ggcacatttc cacaaaatag cggtgaaaac ttaaatcatt
       61 attcacttcg ggatggaagg atatacctat ctgattttgg tattttactc caacaatctt
      121 accgcttatc ttggctaaaa tttctacatc ttttccagtg ctttctatat aaggcgcacg
      181 aatgaatgtg agaggaattt taccgagata tttaacttct tcttctacta aaaacgagcc
      241 taattgcctt ccgtaagcat tacgtttcac tgttacaggc aaagtagcca aatgtttttt
      301 atcgtcgttt accaaatttt ccgcaagcaa aattaaaccc gcacaagttc caagcgttgg
      361 caagccattt aaaatttttt gacgaagcgg ctcaagcaag cctaattcac gtaataattt
      421 accttgaaca gtcgattcgc caccaggtaa aatcagacca tcaaaatctt ggtttaaatc
      481 agcaagttga cgaatttcca tactttcaac ccctagttgc tggagtttat cagtatgctc
      541 gctaaatgca ccttgcagag caagaatagc aattttcatt agacacctct tgctgccatt
      601 agcaattcaa tctcttgctc attaattcct accattgctt cacctaaatc ttcagaaagc
      661 tccgcaagta atttaggatc gttgtagttg gtaacagctt gtacaatggc ccgcgctcgt
      721 ttagcaggat cgcctgactt gaaaatacct gagccaacga agactccttc tgcacctaac
      781 tgcatcatca atgcagcatc ggcaggggtt gcaacacctc cagcagcaaa ggtgacaaca
      841 ggcaattttt ggtactgatg tacatattgt actaaatcaa atggtacttg gagctcttta
      901 gctacgtgat aaagttcatc tgtggtatgt gaaaccaagc gggcaatttc ttgtttcatt
      961 ttgcgaagat gatgtactgc ttgcaccaca ttgcctgttc ctggctcgcc cttactacgg
     1021 atcatagcag cgccttcatt aatacgacgt agggcttcgc ctaaattttt tgctccacat
     1081 acaaatggca cttcaaattt acgcttgtta atgtggaagg tatcatctgc aggagaaagc
     1141 acctcacttt cgtcaatata gtcaatttcg atcgcttgca ggatttgtgc ttctgtaacg
     1201 tgtccaatac gtactttcgc cattacagga atggataccg ctgcttggat actctttatc
     1261 atcttgggat cactcatacg agaaacgcca ccagctgcac gaatatctgc aggaattctc
     1321 tctagtgcca ttactgcaca agctccagct tcttccgcaa tgcaggcttg ctcaggggta
     1381 gttacatcca taattacacc gccttttaac atttgagcaa gttgtctgtt taaatcatag
     1441 cgattagtca ttgtactttt tccttctaat tgttgaataa ttgaactttt cgtagtttcg
     1501 attagctcgg cattaagttt atttttatat tcgctggatg cgagtgttca tctaccaatt
     1561 taaatacact cagccaattt gagtgaggaa acagcatgta ttgtaattta atctgaactg
     1621 tttaaaatga ccattttatg tttttcttat catgtcagat tatgcttacc tacactttta
     1681 acacacaaga aggcaccgct ctctatgagc aactttaccg ttttctaaaa aacgacattg
     1741 aaagcaacat acttatagca ggggataaac tgccgtctaa acgggctttt gcaaagcatt
     1801 taggcatttc agtaatgaca gtggaaacag cttaccaaca gttagtagca gaaggttatg
     1861 tgaatactca gcctaaaaaa ggcttttttg tgaatgcttt aaatttacca gaaaaaaagg
     1921 aagccatatc agttcagcta caaaaaatca gttcagaaca gtatgagaaa aataaaaaat
     1981 ggcaggcaga tttaactaat cgacagactt tagcagaaaa ttttccgttt tcggtttgga
     2041 caaaattagt acgcgaagtg ctgaaaacac accaaagtga gctaatggag cgtgctgaga
     2101 gtgggggcgt atggacatta aaatttgcga tagccaatca tcttcgagcc tttagaggaa
     2161 tgatcgttaa tccaacacag attattattg gagcgggtac ggagtatctt tacggtttgc
     2221 tcattcagct tctaggctta gataaacatt atgcactaac tgagcccagt tacgataaat
     2281 tgcgcagaat ttatcacagt tatcgcttat caacagccac agtatcgatg ccattttcaa
     2341 tcgatgagtt aaattcaaag aatgtggacg tgatacatac ctcgccatca caccattttc
     2401 cgacaggaca cgtaatgccg attgccaaac gttatgagtt gttagcttgg gttgcacaaa
     2461 aagctgaaag atatattgta gaagacgact acgacagcga atttcgtttt gtaggacaac
     2521 caattcctgc attgaaatct attgatatgc tcgggcgagt tatctatatg aatacttttt
     2581 ctaaaacgct ctcttcaact gtacgaattg cctatatggt tttgccacca gaattactga
     2641 atcgttttca gcaggaatta gggttttatg cttctaccgt ttccaatttt gagcaatata
     2701 ttttggcaga atttattcaa caaggctatt ttgaaaagca tatcaatcga atgcgagcgt
     2761 attatcaaaa aaagagggat aaattactaa aagctttcaa gcaaggcaat ttagcagaaa
     2821 agattgcaat aaaagaagaa cattcaggct tgcatttcat tgtacagcta aacacaacat
     2881 taacagaaag gcaaattttg caggcagcag aagaacaagg tataaaattt gtcgcattgt
     2941 ctgcttatta tcaagataaa tctcaaattc agcaaaatgc ttttataatt gggtactcta
     3001 atttaaaaga tgatcagatt aattatgtag cagagagcct gaaaaaaatt ttagacaacg
     3061 aatagcaagt atcaaaactg ctttaatttt tagcaaattg ccttaaaaac acgcaagtgg
     3121 taattatttt agtgattttt gcaaaaaatt accaaattct ccttgcaagg cattttgttt
     3181 tgcgtataat gcatggcaca caacgacgca ctgttgtgaa cgatttaaaa ttgatttcca
     3241 gtgcgtcgtt tctttttgtt ctttaaca
//
LOCUS       DANLXF010000240         3262 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.240, whole genome shotgun sequence.
ACCESSION   DANLXF010000240 DANLXF010000000
VERSION     DANLXF010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.240"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(15..137)
                     /locus_tag="PXD66_002256"
     CDS             complement(15..137)
                     /locus_tag="PXD66_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HDL6320505.1"
                     /translation="MKKLVLTILAAATFLTACNTVDGVGEDVEKAGDKIQQAAQ"
     gene            complement(217..1194)
                     /gene="rluD"
                     /locus_tag="PXD66_002257"
     CDS             complement(217..1194)
                     /gene="rluD"
                     /locus_tag="PXD66_002257"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HDL6320506.1"
                     /translation="MTQQKILTAEITADLLGARLDQALAQLFPDYSRSRIKVWIESDL
                     VKVNGNIVNKAREKVFGGETVEITAEIEEEIRFEPQDIPLNIVYEDDDIIVINKPKDL
                     VVHPGAGNPDGTVLNALLHYYPPIAEVPRAGIVHRLDKDTTGLMVVAKNIPAQTHLVT
                     ALQKRRITREYEAIASGIMTQGGKVDEPMARHPTKRTAMAVHPMGKPAVTHYRIMERF
                     RNYTRLRLRLETGRTHQIRVHMAHIAHPLLGDQLYGGRPRPPKGASDSFLSVLRGFQR
                     QALHATMLRLEHPITGEMMEWHAPLPDDFIELIQALKADYELYKDQLDY"
     gene            1335..2114
                     /locus_tag="PXD66_002258"
     CDS             1335..2114
                     /locus_tag="PXD66_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HDL6320507.1"
                     /translation="MRKFKSLATLVLAGLFVAGCSNSNKELEQSNVQDLYGKGQTYLQ
                     DGDYNSAIRYLEAVGTKGGQYSGLGEQTQLSLIYAQYKVGEYYKALDAAERFARSYPN
                     AANMDYVFYLAGLSNARLSDNFIQDFFGVNRASRAVDNVRNAYGNFQTIVQRYPQSQY
                     AQDAQNWMNYLFNRLAEHELAVVKFYDERNAYVAVVNRIEEMLRFYPNTLATKQALPY
                     LKEAYKQMNIPDAEQKTELLIQEYESKELPNPEKLKYGEQF"
     gene            2187..3218
                     /gene="tsaD"
                     /locus_tag="PXD66_002259"
     CDS             2187..3218
                     /gene="tsaD"
                     /locus_tag="PXD66_002259"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDL6320508.1"
                     /translation="MRILGIETSCDETGVAIYDEDKGLVANQLYSQIDMHADYGGVVP
                     ELASRDHIRKTLPLIQEALKEANLQPSDIDGIAYTAGPGLVGALLVGSTIARSLAYAW
                     NVPALGVHHMEGHLLAPMLEENAPEFPFVALLISGGHTQLVKVDGVGQYELLGESIDD
                     AAGEAFDKTGKLLGLDYPAGVAMSKLAESGTPNRFKFPRPMTDRPGLDFSFSGLKTFA
                     ANTIKANLNENGELDEQTKCDIAHAFQQAVVDTILIKCKRALEQTGYKRLVMAGGVSA
                     NKQLRADLAEMMKKLKGEVFYPRPQFCTDNGAMIAYTGFLRLKNDEQTDLSISVKPRW
                     AMTELPPIN"
BASE COUNT          964 a          705 c          646 g          947 t
ORIGIN      
        1 ttcgaagctt tttgttattg tgcagcctgc tgaattttat cgcccgcttt ttcaacatcc
       61 tcaccaacac catcaacggt attacacgca gtcaagaaag ttgcagctgc taaaatagtt
      121 aatactaatt ttttcataga aagctccttt tttgttgttt acttatgaaa acgatttcaa
      181 tatagcaact ttacattatt taagcaacac ctaaacttaa taatctaatt gatctttata
      241 aagctcataa tccgctttta gtgcctgaat caattcaata aaatcatcag gtaacggggc
      301 gtgccactcc atcatttcac cagtaatcgg atgctctaat ctcaacattg tggcgtgtaa
      361 ggcttggcgt tggaagcctc gtaacaccga taaaaacgag tcactcgcac ctttaggtgg
      421 gcgaggtcta ccgccataga gctgatcacc taataatggg tgagcaatat gcgccatatg
      481 tacccgaatt tggtgagtac gccctgtttc taatcttaaa cgtaaacgtg tgtaattacg
      541 aaaacgctcc ataatacgat aatgcgtaac cgccggtttt cccattgggt gaacagccat
      601 tgccgtacgt ttagttgggt ggcgagccat tggctcatca acttttccgc cctgtgtcat
      661 aatgccgctg gcaatcgctt catattcacg ggtaatacga cgtttttgta atgcggttac
      721 taagtgagtc tgtgccggaa tgtttttagc cactaccatt aagcctgtcg tgtctttatc
      781 caatcggtga acaatccctg ctcgaggcac ttctgcaatc ggtggataat aatgtagcaa
      841 agcattcagc actgtaccgt ccggattacc agcccctggg tgaaccacta aatccttcgg
      901 tttattaatg acgataatat cgtcatcttc atacacaata tttagaggaa tatcttgagg
      961 ctcaaaacga atttcctctt ctatttcagc ggtaatttca acagtttcac caccaaatac
     1021 tttttccctt gctttattca cgatattgcc attaactttc actagatcac tttcaatcca
     1081 cactttaatg cgtgaacgag aatagtccgg aaaaagttgt gccagagcct gatctaggcg
     1141 agcacctagt aaatctgcag taatttctgc tgttaaaatc ttttgttgag tcattaaacc
     1201 ataaatccta attagtcatt tgcttacttg cgtgatgtta tatagaatag cgagatgaac
     1261 ttcataagtt attcttagtc agaggacatt gtacattaac ttttcgtcct agacactttt
     1321 tataggtaaa acatatgcgt aaatttaaat ctcttgccac attagtatta gccggactgt
     1381 ttgttgcagg ttgttcaaat tcaaataaag aattagaaca atccaacgtt caagatcttt
     1441 atggcaaagg tcagacctat ttacaagatg gagattataa ttctgccatt cgctacttag
     1501 aagcggttgg aaccaaagga ggccaatatt caggacttgg tgagcaaacc caactaagtt
     1561 taatttatgc tcaatataaa gttggcgaat actataaagc acttgatgcc gcagaacgtt
     1621 ttgctcgctc ttacccgaat gcagctaata tggattacgt attctatcta gcaggattat
     1681 caaatgcacg tctaagtgat aattttattc aagacttctt tggtgtaaat cgcgcctctc
     1741 gagccgtaga taatgttcgt aatgcttacg gtaacttcca aactattgta cagcgctacc
     1801 ctcaaagcca atatgcacaa gatgctcaaa attggatgaa ttacttattt aaccgtttag
     1861 cggaacacga attagccgtt gttaaatttt atgatgaacg taatgcctat gtggctgtag
     1921 taaaccgtat cgaagaaatg ttgcgttttt acccgaatac actggcaacc aaacaagcat
     1981 tgccatactt aaaagaagca tataagcaaa tgaatatccc agatgcagag caaaaaacgg
     2041 aactcttgat ccaagaatat gaaagcaaag agctaccgaa tcctgaaaaa ctgaagtatg
     2101 gcgaacaatt ctagtcgtac agagaataat gtgaggggcg ttcttcgccc cttttggttt
     2161 tctaacttat tttgacttct ccaactatgc gaattttagg tattgaaacc tcttgtgatg
     2221 aaaccggtgt tgccatttat gatgaagaca aaggcttagt ggcaaaccag ctttatagcc
     2281 aaattgatat gcacgccgat tacggtggcg tagtccctga actggcttct cgagaccata
     2341 tccgtaaaac gttgccacta attcaagaag ccttaaaaga ggccaatctg caaccctcgg
     2401 atattgacgg cattgcctat actgccggcc caggcttggt cggggcttta ttggtcggct
     2461 caaccattgc ccgttcgctg gcttatgctt ggaatgttcc ggcattgggc gttcaccata
     2521 tggaagggca tttacttgcc ccaatgttgg aagaaaatgc ccctgaattt ccgtttgtgg
     2581 cattattgat ttcaggtgga cacacccaac tggtaaaagt tgacggcgtt gggcaatacg
     2641 aactactcgg ggaatcaatt gatgatgctg ccggtgaagc ctttgacaaa acaggcaaac
     2701 tactcggttt ggattaccct gccggtgtag cgatgtcaaa attagccgaa tccggcacgc
     2761 caaatcgttt taaattccct cgtccaatga ccgacagacc gggactggat ttcagtttct
     2821 ccggtttaaa aacctttgct gcgaatacga ttaaagccaa tcttaatgaa aatggtgaac
     2881 tcgatgagca aaccaaatgc gatattgccc acgcattcca acaagccgtg gttgatacta
     2941 ttttaattaa atgcaagcga gcgttagagc aaaccggcta taaacgctta gtaatggcag
     3001 gcggcgtaag tgccaataaa caattacgag cagaccttgc ggaaatgatg aaaaaattaa
     3061 aaggcgaagt attctaccct cgcccacaat tttgcactga caacggcgca atgattgcct
     3121 acactggctt tcttcgctta aaaaacgatg aacaaaccga cttaagcatt agcgtaaaac
     3181 cccgctgggc tatgaccgaa ttaccaccga ttaattaacc tttcaagcgg tgaaatttct
     3241 tgttaatttt gcaaaaattt aa
//
LOCUS       DANLXF010000241         3257 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.241, whole genome shotgun sequence.
ACCESSION   DANLXF010000241 DANLXF010000000
VERSION     DANLXF010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.241"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..139)
                     /gene="rpmG"
                     /locus_tag="PXD66_002260"
     CDS             complement(<1..139)
                     /gene="rpmG"
                     /locus_tag="PXD66_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL6320509.1"
                     /translation="MAAKGAREKIRLVSTAETGHFYTTDKNKRNMPEKMEIKKFDPVV
                     RK"
     gene            complement(151..387)
                     /gene="rpmB"
                     /locus_tag="PXD66_002261"
     CDS             complement(151..387)
                     /gene="rpmB"
                     /locus_tag="PXD66_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004997628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDL6320510.1"
                     /translation="MSRVCQVTGKRPAVGNNRSHAMNATKRRFLPNLHTHRFWVESEN
                     RFVTLRLTAKGMRIIDKKGIDAVLAEIRARGEKI"
     gene            complement(619..1356)
                     /gene="radC"
                     /locus_tag="PXD66_002262"
     CDS             complement(619..1356)
                     /gene="radC"
                     /locus_tag="PXD66_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDL6320511.1"
                     /translation="MEEKMNDFFDEDSQEPTMMPREKLLTQGAASLTDSELLAIFLRT
                     GTKAVPVLALAESVLMAFGSLRQLLNANIDDFCKIYGLGKTKYIQLQASKEMTKRYLA
                     QQMEFSEMIQAPYMAIMYFQTELEEEEREVFMVLFLDNQNRLIYKEKMFFGTINQTAV
                     HPREIIKRALKYNAAAIIVAHNHPSGSCLPSESDRSLTKKIEMACELVDIRFVDHIIV
                     GKGDYFSFEEEKLEMKEHSFLQISDRK"
     gene            1506..2708
                     /gene="coaBC"
                     /locus_tag="PXD66_002263"
     CDS             1506..2708
                     /gene="coaBC"
                     /locus_tag="PXD66_002263"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDL6320512.1"
                     /translation="MLQNKKILVGISGGIAAYKTIEFIRHLKKANAEVRVVLTPAAEA
                     FVTPLTLQAISGNAVSTSLLDPQAELAMGHIELAKWADLIVIAPASADFIARLRMGMG
                     NDLLSTLCLATASPILLAPAMNQQMYKQIAVQENLSVLVNRGVQLIGPNSGFQACGDV
                     GAGRMSEPEEIYQAVLAHFSNNQDLEGLNVTITAGPTREAIDPVRYISNHSSGKMGYA
                     IANAFAERGAKVTLISGPVNLPTPKNVERINVESAVEMEQQAVKFAQKSAIFIGCAAV
                     ADYRMAAVSDQKIKKTDANDELTLKLVKNPDIIANVANLAENRPFVVGFAAETQNVAE
                     YAKSKLERKNLDMICANDVSGGQVFGQDQNALHLFWKNGEKTLPLADKSKLAESLVQE
                     IVKHYREK"
     gene            2708..3025
                     /locus_tag="PXD66_002264"
     CDS             2708..3025
                     /locus_tag="PXD66_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320513.1"
                     /translation="MKLLVSHFHQGEGKFPTFSAGTAVRLGSECVNYPNWFACEIDGW
                     KTFVPSHFISEDKLICDYNPTELVAAQGERVEFLELHYQWAIVKRENEIGWLPCEILR
                     VIG"
     gene            3022..3204
                     /locus_tag="PXD66_002265"
     CDS             3022..3204
                     /locus_tag="PXD66_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HDL6320514.1"
                     /translation="MKIMQLTSNQYINDIKHILLSARQKAYQAVNSAMVEAYWKIGER
                     IVQEEKNMVKDQNEKV"
BASE COUNT          971 a          677 c          680 g          929 t
ORIGIN      
        1 gtttacgcac aactggatca aattttttga tttccatttt ttcaggcata ttacgtttgt
       61 ttttatctgt tgtgtagaag tgaccagttt ctgctgtaga aactaaacgg attttctcac
      121 gagcgccttt agctgccatt ttttagctcc ttagattttt tcgccacgag cacggatttc
      181 agctaatact gcatcaatgc cttttttatc aataatacgc atacctttcg cagttaaacg
      241 taaggttacg aaacggtttt cgctctcaac ccagaaacgg tgagtgtgaa ggtttggtaa
      301 gaaacgacgt ttagtcgcat tcattgcgtg tgagcggttg ttaccaactg ctggacgctt
      361 gccggttact tggcaaactc ttgacatttg aatttctcca ataattttaa gcttaaaaat
      421 ggttcgggcg atctcacaaa aggcgttgct caaccaaccc ctccgatttt gattgcctcg
      481 tcaggcatta acccgaccac agtctatgta ataaagactg ccgattatac cgatttcatt
      541 tgttatattc aagtagaatt tatatgcgta tcttttactg tttcaggaga aatgttaaag
      601 gttacataaa gcaagcggtc attttctatc agaaatttgc aaaaatgaat gctccttcat
      661 ctctaatttc tcttcctcga acgaaaaata gtcccctttg ccgacaataa tatgatccac
      721 aaagcgaata tcaaccaatt cgcacgccat ttcgatcttt ttagttaaac ttctgtccga
      781 ctcactcggc agacaagaac ctgaggggtg attatgagcc acaataatcg ccgcggcatt
      841 atattttaac gctcttttaa taatttctcg ggggtgaaca gcagtttgat taatggtgcc
      901 gaagaacatt ttttctttat aaatcaatcg gttttggttg tccaaaaaca gcaccataaa
      961 aacttcccgt tcttcttcct ccaactcggt ttggaaatac ataatcgcca tataaggggc
     1021 ttgaatcatc tcgctaaatt ccatttgttg agccagatag cgtttggtca tctctttaga
     1081 ggcttgtaac tgaatgtatt tggttttgcc taaaccgtaa attttgcaaa aatcatcaat
     1141 atttgcattc agtaattggc gtaacgagcc aaatgccatc aacacactct ctgccaatgc
     1201 cagcacaggc acagctttcg taccagtccg taaaaatatc gctaataatt cggaatcggt
     1261 taatgaggct gccccctgtg ttaataattt ctcacgcggc atcattgttg gctcttgaga
     1321 atcttcatca aaaaaatcgt tcattttttc ctccatcaat ttagttaagc ataaaagagc
     1381 caacctttgc agacaaaggg atttattcaa tttttcgagc aagatcgcaa aaatagtgaa
     1441 gttgtgagca actccttatt ccgctaaaat atgcaaaata aatccacgct ttaaggatag
     1501 taaagatgtt acaaaacaaa aaaattttag tcggcatttc cggtgggatt gcagcctaca
     1561 aaaccattga atttatccgc cacttaaaaa aggcaaatgc ggaagtgcgt gtagtgctaa
     1621 ccccagcagc agaagccttt gtaacgcctt tgaccttaca agcgatttcc ggcaatgcgg
     1681 tttccacttc actactcgac ccacaagccg aactggcgat ggggcatatt gagctggcaa
     1741 aatgggcgga tttaatcgtt atcgccccgg caagtgccga ttttattgct cgcctgcgta
     1801 tgggaatggg caacgattta ctttccactc tctgccttgc gactgcaagc ccgattttgc
     1861 ttgcaccggc aatgaatcaa caaatgtaca aacagattgc ggtgcaagaa aatctgtctg
     1921 ttttagtcaa tcgtggcgtt cagctcatcg gcccgaatag tggcttccaa gcctgtggtg
     1981 atgtgggggc tggcagaatg tctgagcctg aagaaattta ccaagcggtg ttggctcatt
     2041 tctccaacaa tcaagattta gagggcttaa atgtaaccat taccgccgga ccgactcgtg
     2101 aggcgattga ccctgtgcgt tatatcagca accacagctc cggcaaaatg ggctacgcga
     2161 ttgctaatgc ctttgccgaa cgtggagcaa aggttacgtt gattagcggt ccggtgaatt
     2221 taccaacgcc gaaaaatgtg gaacgaatta atgtggaatc tgcggttgag atggagcaac
     2281 aagcggtcaa atttgcccaa aaatctgcga tttttattgg ctgtgcagcg gttgccgatt
     2341 accgaatggc ggcagtctct gaccaaaaaa ttaagaaaac cgatgccaat gatgaactca
     2401 cgctaaaatt ggtaaaaaac cccgatatta tcgctaacgt cgccaatctt gccgaaaacc
     2461 gcccttttgt ggttggtttt gccgcagaaa cgcaaaatgt ggcggagtac gctaaatcga
     2521 aactcgaacg caaaaatctg gatatgattt gtgccaatga tgtctcaggc ggacaagtgt
     2581 tcgggcagga tcaaaatgcg ttgcatttgt tctggaaaaa tggtgaaaaa acgcttcctc
     2641 tggcggataa aagcaaattg gcagaaagtt tggtacagga aattgtaaaa cattatcgag
     2701 aaaaataatg aaattactgg ttagccattt tcatcaaggc gaagggaaat ttcctacatt
     2761 ctcagctggt actgccgttc gattagggag tgaatgtgta aactacccaa actggtttgc
     2821 ctgtgaaatt gacggttgga aaacctttgt gccaagccat tttattagcg aagataaatt
     2881 gatatgcgat tataacccaa cggaacttgt tgcagcacaa ggcgagagag tagaattcct
     2941 tgaactacat taccaatggg caattgtcaa aagagaaaac gaaattggct ggctaccttg
     3001 tgaaatctta agggtaatag gatgaaaatt atgcaactta cgtcaaatca gtatatcaac
     3061 gacattaaac atattctttt atctgctcgc caaaaggcgt atcaagccgt gaattcggca
     3121 atggtcgaag cctattggaa aatcggtgaa cgaattgtgc aagaagagaa aaatatggtc
     3181 aaagatcaaa atgaaaaagt ataatattgt attttcagca gataaaaact acctgccata
     3241 tttagagatt gccttaa
//
LOCUS       DANLXF010000242         3238 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.242, whole genome shotgun sequence.
ACCESSION   DANLXF010000242 DANLXF010000000
VERSION     DANLXF010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.242"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..575)
                     /locus_tag="PXD66_002266"
     CDS             complement(<1..575)
                     /locus_tag="PXD66_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL6320515.1"
                     /translation="MQERITQQNERLSAYMDGQNVDTAFVETLTNSPELQQKWASYHT
                     IRSVMQGDEIILGRDFSAKMEALLENEEIESQANVEKPKGLLLKLKRWGTPIMQAGIA
                     ASVCLVAVLGVNSMNASDEIAQTQQPVLQTLPFSNSVEAVSYNAPVQDQPTEEQLELQ
                     QRKINALLENHELQRRTNNVKNLTLSEEEKQ"
     gene            complement(621..1193)
                     /gene="rpoE"
                     /locus_tag="PXD66_002267"
     CDS             complement(621..1193)
                     /gene="rpoE"
                     /locus_tag="PXD66_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HDL6320516.1"
                     /translation="MSEQITDQELVEKAQKGDKKAFNLLVVRYQNRVAGLLTRYVARD
                     DIPDIVQESFIKAYRSLESFRGESAFYTWLYRIAVNTAKNHLTSLGRRPPKEDILAED
                     AESYDVGAQLRESDTPENLILSEELKKVVFDTIENLPDELKTAIRLRELEGLSYEEIA
                     EVMECPVGTVRSRIFRAREAIDAKIKPLMQ"
     gene            complement(1415..2959)
                     /gene="leuA"
                     /locus_tag="PXD66_002268"
     CDS             complement(1415..2959)
                     /gene="leuA"
                     /locus_tag="PXD66_002268"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDL6320517.1"
                     /translation="MSNNNIIIFDTTLRDGEQSLKASLTVKEKLQIALALERLGVDVM
                     EVGFPVSSAGDFESVQTIARHIKNSRVAALSRAVAKDIDAAYEALKVAEAFRIHTFIA
                     SSALHVEAKLKRSFDDVVEMAVAAVKRARNYTDDVEFSCEDAGRTGIDNICRIVEAAI
                     NAGATTVNIPDTVGFCLPNEYGNIIHQVMNRVPNIDKAIVSVHCHNDLGMATANSLTA
                     VQNGARQIECTINGIGERAGNTALEEVVMAIKTRQEFMGVDTRINTQEIHRVSQMVSQ
                     LCNMPIQPNKAIVGSNAFAHSSGIHQDGMLKNKNTYEIMSPETIGLKKEKLNLTARSG
                     RAAVKGHMADMGYTETDYDLDKLYEAFLQLADKKGQVFDYDLEALAFIDMQQGDEDRL
                     KLDVITTQLISGLPASAFVQVELDGVRQSATSQGGNGPVEATYNAIMNIVGMELKMSH
                     YNLTAKGEGAEALGQVDIVVEYEGRKFHGVGLATDIVESSARALIHAINAIYRSQKVA
                     DLKAKK"
BASE COUNT          881 a          717 c          574 g         1066 t
ORIGIN      
        1 ttttgctttt cttcctcaga taaggttaaa tttttaacgt tattagtacg gcgttgtaac
       61 tcatggtttt ctaataacgc attaatctta cgctgttgaa gctctaactg ttcttctgtt
      121 ggttgatctt gaactggcgc attataactt accgcttcaa ctgaattgct aaatggcaat
      181 gtttgtaata ctggttgttg cgtttgagct atttcatcac tcgcattcat tgaattaacc
      241 cctaataccg caactaagca cactgaagct gcaataccgg cttgcataat tggagttccc
      301 caacgcttga gcttgagcaa taagccttta ggtttctcta catttgcttg agattcaatt
      361 tcttcatttt ccagtaatgc ttccatttta gcagagaaat ctctccctaa aatgatctca
      421 tcaccttgca tcacactacg aatagtgtga taacttgccc atttttgctg taattcaggg
      481 ctgttagtta atgtttctac aaatgctgta tcaacatttt gcccatccat ataagctgaa
      541 aggcgttcat tttgttgagt gatgcgttct tgcataattg actccaatga tttaaatctc
      601 ttctaaaata atctttctat ctattgcata agtggcttga ttttagcatc aatcgcttcc
      661 cttgctcgga aaatacggga acgtacagta ccaaccggac attccatcac ttctgcgatt
      721 tcttcgtaac ttagcccttc caattctctt aaacgaatgg cggttttaag ctcatcaggt
      781 aaattttcaa tcgtatcaaa cacgactttc tttaattcct cggataatat caaattctct
      841 ggcgtatcac tttcacgaag ttgcgcgcct acatcataac tttcagcgtc ttcggcaaga
      901 atatcttctt ttggcggtct tctgcctaaa gaggttaaat gatttttcgc agtattcact
      961 gcaatgcgat aaagccacgt ataaaaagca ctttcgccac ggaaagactc taaagaacga
     1021 taggctttaa taaaagattc ttgcacaatg tccggaatat catctcttgc aacataacga
     1081 gtcagcaaac cggctactct attttgataa cgtacaacaa gtaaattaaa tgctttttta
     1141 tcaccttttt gtgctttttc gactaattct tggtcggtga tctgttcgct caactgcgga
     1201 ctctcctaaa tatcaatatg tcattttcca ttgcacactt tgttagtgtg tgctttatga
     1261 caaaagttca aaaaatttga aaaattgtgt aattttttac attcctagat aaaacctact
     1321 taataaacta agcaagcggt cggattttag cgaaattttg caaaaatttc aaaaaatccg
     1381 accgcttgtc tatgttatgc tttcgctcta taagttattt cttcgctttt aaatctgcca
     1441 ctttttgtga acggtaaatc gcattaatgg cgtgaattaa agctcgggct gaagattcca
     1501 caatatcggt tgccaaaccc acaccgtgga attttcgacc ttcatattcc actacaatat
     1561 ccacttgccc taatgcttct gctccttcgc ctttcgcagt cagattatag tgcgacattt
     1621 ttagctccat tcccacaatg ttcataatgg cattgtaagt tgcctcaact ggaccatttc
     1681 ccccttgaga tgtagcactt tgacgaacgc cgtctaattc cacctgtaca aaagccgaag
     1741 ccggtaaacc gctgattagt tgggtggtaa ttacatctaa ttttaagcgg tcttcatcgc
     1801 cttgctgcat atcaatgaag gcaagagctt ctaagtcata atcaaacact tgcccttttt
     1861 tatcagcaag ttgcaagaag gcctcataaa gtttatctaa atcgtaatcc gtttccgtat
     1921 aacccatatc agccatatgc cctttcaccg ctgctctacc tgaacgggca gttaaattca
     1981 gcttctcttt tttcaagccg atggtttccg gcgacataat ttcataagta tttttatttt
     2041 tcagcatacc gtcttggtga atacctgaag agtgagcaaa ggcattgcta ccgacaatcg
     2101 ctttgttcgg ctgaatcggc atattgcaaa gctggctgac catttggctg acacggtgaa
     2161 tttcttgggt attaatgcgg gtatcaaccc ccataaattc ttggcgggtt ttaattgcca
     2221 ttaccacttc ttcaagagcg gtgttaccgg cacgttcgcc aataccgttg atagtacact
     2281 caatctgtct cgctccgttt tgcaccgctg tcaatgagtt ggcggtagcc atacctaaat
     2341 cgttatggca gtgaacagag acaatcgctt tatcgatatt cggaacacgg ttcatcactt
     2401 ggtggataat attgccgtat tcattcggta aacagaaacc gactgtatcc ggaatattca
     2461 ctgtggttgc accggcatta atggcagctt ctacaatgcg gcagatgtta tcaattccag
     2521 tacgcccagc atcttcacag gaaaactcaa catcatcagt ataatttctg gcgcgtttta
     2581 ccgctgcaac cgccatttcg actacatcat caaatgaacg ttttaattta gcctcaacgt
     2641 gtaatgccga gctggcaata aaggtatgga tacggaatgc ttctgctact tttaaggctt
     2701 cataagctgc atcaatgtct tttgcaaccg ctcgggaaag cgccgccacc cggctgtttt
     2761 tgatatgacg agcgatggtt tgtactgatt caaaatcgcc cgctgaagaa actggaaaac
     2821 cgacttccat cacatcaacc cctaaacgtt ctaaagcaag ggcaatttgc aatttttctt
     2881 ttacggttaa acttgctttt aatgattgct caccatcacg taaagtcgta tcaaaaataa
     2941 taatgttatt gttgctcata gaagcctctt tttaggtgtt tttatttgat aaaaaaagcc
     3001 cgcattttaa ggtgcgggca agaatgtgga tagtccattt actttttcca atcaatcttt
     3061 atcctcgcac aaaatacgat aacagagata atcggagtaa ggtaaatttt tccatgttgt
     3121 gttgtatgtt tatgtttgca ttgttattca gtagttgagg tgtatattat ccttatccaa
     3181 atcaatgtca atagcatttt tctcttcacg caaccgttta ccagcccatt aattcaag
//
LOCUS       DANLXF010000243         3232 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.243, whole genome shotgun sequence.
ACCESSION   DANLXF010000243 DANLXF010000000
VERSION     DANLXF010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.243"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            34..174
                     /locus_tag="PXD66_002269"
     CDS             34..174
                     /locus_tag="PXD66_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320518.1"
                     /translation="MKKLLLCVGILVLAACSSTGVGGSIGAGGGSNGVGIGLGVGTDF
                     RF"
     gene            233..1387
                     /gene="tgt"
                     /locus_tag="PXD66_002270"
     CDS             233..1387
                     /gene="tgt"
                     /locus_tag="PXD66_002270"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDL6320519.1"
                     /translation="MKYELKTTSGNARRGRLTFNRPKGEFHVETPAFMPVGTYGTVKG
                     MTPEEVKATGAQILLGNTFHLWLRPGQEVMKMHGDLHDFMQWHGPILTDSGGFQVFSL
                     GKLRKIKEEGVTFQNPISGEKIFLSPEKSMEIQYDLGSDIVMIFDECTPYPATFDYAK
                     KSMEMSLRWAKRSRDRFDELENPNALFGIIQGGIYEELRKVSVEKLVEIGFDGYAVGG
                     LAVGEPKEDMHRILEYVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNA
                     RNGHLFVTNGIVKIRNAKYRADTTPLDPECDCYTCKNYTKAYLYHLDKCGEILGARLN
                     TIHNLRYYQRLMAEIRQAIEEDRFDDFVVEFYARIGKEVPPLQSEVKEQK"
     gene            1400..1912
                     /locus_tag="PXD66_002271"
     CDS             1400..1912
                     /locus_tag="PXD66_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HDL6320520.1"
                     /translation="MQQLEPQTFASWAEPIDMLYACHSKVKSFCKQLQILPDYLEKNG
                     VNQAVKNDVQQILNYFNISAPLHHEDEEDDFFPELIKVQPQAQAEVDELERQHVHLHK
                     NWDYLSAQLEDLLAGKRENVDRDLIERFVAGYDVHIAIEEPLFELGREHLAQDKLSAM
                     GKVMADRRKL"
     gene            1971..2171
                     /locus_tag="PXD66_002272"
     CDS             1971..2171
                     /locus_tag="PXD66_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HDL6320521.1"
                     /translation="MQYVLDLTGYACPLPLLMAKKAMNDLKKGDSLEILLNQQSSLTD
                     FELLAKEQGWLCELQAVENVGY"
     gene            complement(2367..2618)
                     /locus_tag="PXD66_002273"
     CDS             complement(2367..2618)
                     /locus_tag="PXD66_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL6320522.1"
                     /translation="MAPTISDGDQMLVDFSKKEARGDDKIYLVQNGESVWVKRVRKVW
                     DYVELISDNESYRPIRITADEAQNLQIIGQVVHIGHSLV"
     gene            2717..>3232
                     /locus_tag="PXD66_002274"
     CDS             2717..>3232
                     /locus_tag="PXD66_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL6320523.1"
                     /translation="MLDSLKEVNQKLDKKADKVGGGTKEDALVTLDTLIASGTVKAET
                     AMSLLPTLQKGAVATGASSEDMAKIAISSMQQFGIKEEDIGRALDMAVAAGQAGSFEL
                     AYMASWLPQQMAAAKQAGLSSIEGFERLLIANQQARVTAGTSDEAGNNLVNLLGKITA
                     KETNERFKNIEY"
BASE COUNT          957 a          608 c          762 g          905 t
ORIGIN      
        1 atagactctc tttttcgttt aggacagcaa aaaatgaaaa aactcttatt atgcgttggc
       61 attttagtgt tagctgcttg ctcatcaacc ggtgtgggtg gcagtattgg agccggtggt
      121 ggctctaacg gcgttggaat cggcttaggg gtcggcaccg actttcgatt ctaattggta
      181 aatttttggt ggtttttgac cgctttcacg aactgtttat ttgtggatta aaatgaagta
      241 cgaattaaaa acaacaagtg gtaacgctcg tcgtgggcgt ttaactttta accgcccgaa
      301 aggcgaattt cacgttgaaa caccggcatt tatgcctgtt ggcacttatg gcacggtaaa
      361 aggaatgact cctgaagaag ttaaagcgac aggggcgcaa attttattag gcaacacttt
      421 ccatttatgg ttaagaccgg ggcaagaagt gatgaagatg cacggtgact tgcacgattt
      481 tatgcaatgg cacggcccta ttttaaccga ctccggcggt ttccaagttt ttagcttagg
      541 taaactgcgt aaaattaaag aagaaggcgt aactttccaa aacccgatta gcggtgagaa
      601 aattttcctt tcgccggaaa aatcaatgga gatccaatac gatcttggat cagacattgt
      661 aatgattttt gatgaatgta ctccttatcc ggcaaccttt gattatgcaa aaaaatcgat
      721 ggaaatgtca ctacgttggg cgaaacgttc tcgtgatcgc tttgatgaac ttgaaaaccc
      781 gaatgcgtta tttggcatta ttcaaggtgg tatttatgaa gaacttcgca aagtctcggt
      841 ggaaaaatta gttgaaatcg gctttgatgg ctatgcagtc ggtggcttgg cggtaggtga
      901 acctaaagaa gatatgcacc gcattttaga atacgtttgc ccgcaaattc cggcagataa
      961 accacgctat ttaatgggtg taggcaagcc agaagattta gtcgaagggg ttcgccgtgg
     1021 tattgatatg tttgactgcg taatgccaac ccgcaatgcc cgtaacggac atttatttgt
     1081 gaccaacggc attgtaaaaa ttcgtaatgc gaaatatcgt gcagacacca caccgcttga
     1141 tcctgaatgt gattgttaca cttgcaaaaa ctacaccaaa gcctatttat atcacttaga
     1201 caaatgtggc gagattttag gggcaagact aaacaccatt cataatttac gctactatca
     1261 acgcctaatg gcagaaattc gccaggccat cgaagaagat cgttttgatg attttgtagt
     1321 ggaattttat gcccgaattg gcaaagaagt gccaccttta caatctgaag taaaagaaca
     1381 gaaataacaa ggagtgtata tgcaacaact tgaaccacaa acctttgcaa gctgggcaga
     1441 gccgattgat atgctttatg cttgccacag caaagtaaaa agtttctgta aacagttgca
     1501 aattttgccg gattaccttg agaaaaacgg ggtaaaccaa gcagtgaaga atgacgtgca
     1561 gcagattttg aactacttca acatttctgc ccctttacat cacgaagatg aagaagatga
     1621 tttcttccct gagcttatta aggtgcaacc acaagctcaa gcagaggtag acgaactgga
     1681 acgccagcac gttcatttac ataaaaattg ggactatttg tcggcacagc ttgaagatct
     1741 gctcgcaggt aaacgtgaaa atgtggatag agacttaatt gagcgttttg tggcaggtta
     1801 tgatgtgcat attgcgattg aagagccact ctttgagctt gggcgagagc atttagctca
     1861 agacaaatta tcggctatgg gcaaagtgat ggcagaccgc cgtaaactct aatcttttgt
     1921 gattgggcga atttcaatat tcgccctttt tctttattcc ctatttcgct atgcaatatg
     1981 ttttagattt aaccggctat gcctgcccct tgccgttatt aatggcaaaa aaagcgatga
     2041 acgatcttaa aaagggcgat agcttggaaa ttttactcaa tcagcagagt agccttaccg
     2101 attttgaact gcttgccaaa gagcaaggtt ggctttgtga gctacaagcg gttgaaaatg
     2161 tggggtactg atcttttttg catagagaga aagaaaaaaa tttgggcgga aaaataacgg
     2221 cgagccccgt gaatacaagg gttcgccgtt attgtttggt tggggctatg caaaaaacaa
     2281 aatgagcagg attagaaaaa aatgggcagt ttttgcgata ttttttgcat aggtttgatc
     2341 ggttttaaaa ctattttaaa tctgttttaa actaaagagt gtccgatgtg gactacctgc
     2401 ccgatgattt gtaagttttg agcttcgtct gctgtaattc ggattgggcg gtaggactca
     2461 ttatcgctaa tcaattcaac gtagtcccac acttttctta cacgttttac ccaaacgctt
     2521 tcaccatttt gcacaagata aatcttatcg tctccacggg cttctttttt gctgaaatcc
     2581 actaacattt gatcaccatc gctgatagtc ggggccattg atgtgccatt agcccaaaat
     2641 acgccacaat tatgggcttt tacgcctagg cttaataggc tgtctgaata aggctcttaa
     2701 ggaaaagctg gacgatattt tagactcgct caaagaagta aaccaaaaac ttgataaaaa
     2761 ggcggataag gttgggggtg gcacaaaaga ggatgcgtta gttacgcttg atacgctcat
     2821 tgcctccggt acagtcaaag ccgaaaccgc aatgagtcta ttacctactt tgcagaaagg
     2881 ggcggtggca acaggggcaa gctcggagga tatggcaaaa attgcgatct cctctatgca
     2941 acaatttggc atcaaagaag aggatattgg gcgggcattg gatatggctg ttgcagccgg
     3001 gcaagcaggt agttttgagc ttgcatatat ggcaagttgg ttgcctcaac aaatggcagc
     3061 tgcaaaacaa gccggtttga gtagtattga gggctttgag cgacttttaa ttgcaaacca
     3121 acaggctcgt gtcactgccg gtacaagcga tgaggcaggt aataatctgg taaatctgct
     3181 tggtaagatt acagctaaag aaacaaatga gcgttttaaa aatattgaat at
//
LOCUS       DANLXF010000244         3193 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.244, whole genome shotgun sequence.
ACCESSION   DANLXF010000244 DANLXF010000000
VERSION     DANLXF010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.244"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            78..530
                     /gene="iscR"
                     /locus_tag="PXD66_002275"
     CDS             78..530
                     /gene="iscR"
                     /locus_tag="PXD66_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HDL6320524.1"
                     /translation="MKLTSKGRYAVTAILDIALHGKLEPVSLADISDRQAISLSYLEQ
                     LFAQLRRDGIVQSVRGPGGGYQLGKKPEEISVGMIISAVNENIDVTKCKGSGNCRKDS
                     QCLTHSLWERLEDQIEQFLGTISVADLAAEHADHDCEKEHCHDHPHHH"
     gene            585..1805
                     /locus_tag="PXD66_002276"
     CDS             585..1805
                     /locus_tag="PXD66_002276"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HDL6320525.1"
                     /translation="MKLPIYLDYAATTPMDERVAKKMMEYMTKDGIFGNPASRSHKFG
                     WEAEEAVDVARNQIADLIGADSREIVFTSGATESDNLAIKGAAHFYQTKGKHIITVKT
                     EHKAVLDTCRQLEREGFEVTYLDPEEDGILDLAKLKAAIRPDTILVSVMHVNNEMGVI
                     QPIKEIGAICRAKKIIFHVDATQSVGKIEVNVQDLNVDLMSFSSHKLYGPKGIGGLYV
                     CRKPRVRLEAIIHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMARLTVLRDR
                     LYNGFKDIEEVYVNGSMEEGKRIGSNLNISFNFVEGESLMMSLRDIAVSSGSACTSAS
                     LEPSYVLRAIGRDDELAHSSIRFTLGRWTTEEEIDHTIEIVKKAIVKLRELSPLWEMF
                     KEGVDLSKIEWNHH"
     gene            1932..2315
                     /gene="iscU"
                     /locus_tag="PXD66_002277"
     CDS             1932..2315
                     /gene="iscU"
                     /locus_tag="PXD66_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HDL6320526.1"
                     /translation="MAYSEKVIDHYENPRNVGTFDKEASDTGTGMVGAPACGDILRLQ
                     IKVNDQGIIEDARFKAYGCGSAIASSSLITEWVKGKSLEEAGAIKNSDIAEELELPPV
                     KVHCSILAEDAIKAAIADYKEKQAK"
     gene            2424..2747
                     /gene="iscA"
                     /locus_tag="PXD66_002278"
     CDS             2424..2747
                     /gene="iscA"
                     /locus_tag="PXD66_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HDL6320527.1"
                     /translation="MSITLTEAAANRVRTFLENRGKGIGLRLGVKTSGCSGLAYVLEF
                     VDVLNEDDQVFEQHGVKVIVDEKSLTYLNGTQLDFVKEGLNEGFKYTNPNVKNECGCG
                     ESFSV"
     gene            2757..>3193
                     /gene="hscB"
                     /locus_tag="PXD66_002279"
     CDS             2757..>3193
                     /gene="hscB"
                     /locus_tag="PXD66_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HDL6320528.1"
                     /translation="MNNPFTLFDLPVQFQLDNAQLSERYLALQKQLHPDNFAHKSSAE
                     QLEAVQKSADVNEALNILKDPILRAEAMIEIHTGEAKNLEEKSMRDVDFLMQQLEWHE
                     KLETIEHRKDETELTVFLKQIKAEQKAVLEQLEQTLNTADWQKA"
BASE COUNT         1041 a          567 c          709 g          876 t
ORIGIN      
        1 ttgactaaat tggtggggta tatcaaaatt tagcaactgt ttcagaagat tttctggata
       61 atcaatgagg atattttatg aagcttacct caaaagggcg ttatgcagta accgccattt
      121 tagatattgc tttacacgga aaactagagc cggttagctt agccgatatt tcagatcgcc
      181 aagcgatttc actgtcttat ttagagcaac tgtttgctca acttcgccgt gacggtatcg
      241 tgcagagcgt tcgtggaccg ggtggtgggt atcaattagg taaaaaaccg gaagagatta
      301 gcgtgggtat gatcatctcc gccgtgaatg aaaatattga tgtcaccaaa tgtaaaggta
      361 gtggtaattg ccgtaaagat tctcagtgtt taacgcattc tttgtgggag cggttagaag
      421 atcaaattga gcagttttta gggacaattt cagttgcgga tttagccgca gaacacgctg
      481 atcacgattg tgaaaaagag cattgtcacg atcatcctca tcatcactaa ttgcaaattt
      541 tttatcaaaa atgaccgctt gggttgtttt aactaggagt aacaatgaaa ttaccaattt
      601 atttggatta cgcagcaacc acaccaatgg acgaacgtgt tgcgaaaaaa atgatggaat
      661 atatgaccaa agacggtatc tttggtaacc ctgcttctcg ctcacacaaa tttggttggg
      721 aagcggaaga agcggttgat gtggctcgta accaaattgc cgatttaatc ggtgcagaca
      781 gccgtgaaat cgttttcact tctggtgcaa cagagtcgga taacttagcc atcaaaggtg
      841 cggctcattt ctatcaaacc aaaggtaaac acattatcac tgttaaaacc gaacataaag
      901 cagtattaga tacttgccgt cagttagagc gtgaaggttt tgaagtgact tacttagacc
      961 ctgaagaaga cggtatttta gatttagcta aattaaaagc ggctattcgt ccggacacaa
     1021 ttttagtttc ggtaatgcac gtaaataacg aaatgggcgt aatccaaccg attaaagaga
     1081 tcggtgcaat ctgccgtgcg aagaaaatca ttttccacgt tgatgcgacc cagtctgttg
     1141 gtaaaatcga agtgaatgta caagacttaa atgttgattt aatgtctttc tcaagccaca
     1201 aattatacgg accaaaaggt atcggcggtc tatatgtttg tcgtaaacca cgtgtgcgtt
     1261 tagaagcaat tattcacggt ggcggtcacg agcgtggtat gcgttcgggt acattgcctg
     1321 ttcaccaaat tgtaggtatg ggtgaagcat atcgtatcgc taaagaagaa atggcaacgg
     1381 aaatggctcg tttaaccgtg ttacgtgatc gtttatataa cggctttaaa gatattgaag
     1441 aagtatatgt aaacggttca atggaagaag gcaaacgtat cggttcaaac ctaaacatca
     1501 gctttaactt tgttgagggc gaatcgttaa tgatgtcatt acgtgatatc gcagtatcat
     1561 caggttcagc ttgtacttct gcaagtttag agccatctta tgtgttacgt gcaatcggtc
     1621 gtgacgatga attagctcac agctcaatcc gtttcacttt aggtcgttgg acgactgaag
     1681 aagaaatcga tcatactatt gaaatcgtga aaaaagctat cgtgaaattg cgtgagcttt
     1741 cgcctctttg ggaaatgttc aaagaaggtg tagatttgtc taaaatcgaa tggaatcacc
     1801 actaattcag aaaaattttg caagggcaaa taatatttgc ccctacaaaa taagctttca
     1861 tcacaaatag taaattgaat gtaggggcga aataggtttc gccggaaata ggaaataaaa
     1921 ggaatacaaa aatggcatat agcgaaaaag taattgatca ttacgaaaac ccacgcaacg
     1981 tagggacttt tgataaagaa gcatcagaca ccggtacagg tatggtggga gcaccggctt
     2041 gtggtgatat tttacgttta caaattaaag tgaacgatca aggtatcatt gaagatgcac
     2101 gctttaaagc atacggttgt ggttctgcaa tcgcatcaag ttctctcatt accgaatggg
     2161 taaaaggtaa atcattagag gaagcaggtg cgattaaaaa cagtgatatc gctgaagagt
     2221 tagaattacc accggtaaaa gttcactgtt caatcttagc agaagatgca atcaaagctg
     2281 caattgcaga ctacaaagag aaacaagcta agtaatatga tacaatcaat gcaagcggtc
     2341 gatttttgca aaaaatttgc aaaatttaac cgcttgcatt tgctatttct atcttgattt
     2401 aatccattca cataaggaaa actatgagca ttaccttaac agaagctgcc gcaaaccgag
     2461 ttcgtacatt cttagaaaac agaggtaaag gtatcgggct aaggcttggt gtgaaaactt
     2521 ccggttgttc ggggcttgcc tatgtgcttg aatttgttga tgtattaaat gaagacgatc
     2581 aggttttcga acaacacggc gtaaaagtta ttgtcgatga aaaaagcctg acttacttaa
     2641 acggtacgca gctggatttt gtgaaagaag gcttaaacga aggctttaaa tacaccaacc
     2701 cgaatgttaa aaacgaatgc ggctgtggcg aaagttttag tgtgtaagga actgtaatga
     2761 ataacccttt tactttattt gatttacccg ttcaatttca acttgataac gcccagcttt
     2821 ctgagcgtta tcttgcttta cagaaacagc ttcacccaga taactttgcc cacaaatcaa
     2881 gtgctgaaca gttagaagcg gtgcaaaaat ctgctgatgt caatgaggca ctcaatattc
     2941 ttaaagatcc gattttacgg gcagaagcga tgattgaaat ccatacaggt gaagccaaaa
     3001 atctcgaaga aaaaagtatg cgagatgtgg atttcttaat gcagcaactc gaatggcacg
     3061 aaaaactgga aaccattgag catcgtaaag acgaaaccga actgaccgtc tttttaaaac
     3121 agattaaagc ggaacaaaaa gcagtattag aacagcttga acaaacgtta aatacagccg
     3181 actggcaaaa agc
//
LOCUS       DANLXF010000245         3148 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.245, whole genome shotgun sequence.
ACCESSION   DANLXF010000245 DANLXF010000000
VERSION     DANLXF010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.245"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(25..1209)
                     /locus_tag="PXD66_002280"
     CDS             complement(25..1209)
                     /locus_tag="PXD66_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HDL6320529.1"
                     /translation="MTSSNTIKQLDKDYIAQTYGRFDLALSHGKGCEVWDFEGNKYLD
                     FTSGIGVNSLGWADEDWLQAVTTQAGKLAHTSNLFFTEPASQLAQKLVAASGLKRAFF
                     CNSGAEANEGAIKTARKYSFDKYGSGRSTVLTLVNSFHGRTISTLASTGQEVFHKHFF
                     PFTEGFEYLPVNGIEALKARLETENVCGILLEVVQGEGGVCPLEADYLHAVQSLCQEK
                     DILFMIDEVQTGIGRTGTLFAYQQFDLTPGLVTLAKGLGGGLPIGAFLLGEKVEMTLG
                     KSDHGSTFGGNPVSCAAANVVIDKINAEFLAQVQQKGQLLQQRLASLPKVKSVSGLGL
                     MLGVEFEEGILAADIVAKCINKGVLFLTAKSKLRLLPPLMISEQQIEQGIRVLEDVLK
                     DS"
     gene            complement(1222..2076)
                     /gene="argB"
                     /locus_tag="PXD66_002281"
     CDS             complement(1222..2076)
                     /gene="argB"
                     /locus_tag="PXD66_002281"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDL6320530.1"
                     /translation="MQQQEINQFANLLEKSTACLVKWQGKIIVVKYGGNAMINEELKK
                     SVMADLLLLHRLGVKVVLVHGGGLEISQGVKLLGKEPQFINGLRVTDSDTINVVLQML
                     AGKVNKSLVALLKGKGIGLCGIDGAMLQCKKLEAEVDYGFVGDIVKVDPTVIEIALNA
                     GLIPVVSTVGVDENGQAYNINADTAASEIAIALQAEKLVSMTDIAGLLRDRFDESTLI
                     PEVEVSEVQSLIEQGIIAGGMIPKIACCTDFIRAGGSEANIIDGRIDHAILVEMFGGK
                     NGTRFYKK"
     gene            complement(2168..3109)
                     /gene="argC"
                     /locus_tag="PXD66_002282"
     CDS             complement(2168..3109)
                     /gene="argC"
                     /locus_tag="PXD66_002282"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDL6320531.1"
                     /translation="MSKYKIFVDGAVGTTGLRIHERLSQADDIELLTLPEEQRKDLNA
                     RVAKVAEADLTFLCLPDEASKELVANAPENTRICDTSTAHRVNPNWVYGFPELSGQRE
                     KIQNAKRVAVPGCHATGYIALVRPLVEKGILPADYPLSCHSLTGYSGGGKAMIADYEN
                     ENSPNAFNAPRVYGLTLQHKHLPEMQAVTQMQNPPIFTPVVADYYSGMLVTVPLQIFA
                     LQAVKSGEEIANLLADYYQTSRLIHVHPFQSLPEDGMLAANAYAGKDSLEIFIYGNST
                     QLLLAARFDNLGKGASGAAVQCMNIMLGRDEVEGLEL"
BASE COUNT          819 a          791 c          630 g          908 t
ORIGIN      
        1 gatattccta ctgtttataa taactcaaga atctttcaag acatcctcta acactctgat
       61 tccttgttct atctgttgtt cagagatcat cagcggtggg agtagcctta acttgctttt
      121 agcggttaaa aacagcacac ctttgttgat acattttgca acaatatcag cggctaaaat
      181 gccttcctca aattctacgc cgagcattaa acctaagcct gaaacggatt taacttttgg
      241 taagctcgct aagcgttgct gtaacaactg ccctttttgt tgcacttggg cgagaaattc
      301 ggcgtttatt ttatcgatta ctacatttgc agcagcacag gaaacaggat tgccaccaaa
      361 ggttgagccg tggtcgcttt tgcctaatgt catttccact ttttcgccta acaggaaagc
      421 accaatcggc aagccgccgc ccaagccttt agcgagcgtg actaaaccag gagttaaatc
      481 aaattgttga taagcaaaaa gtgtgcccgt tcgcccaatg ccggtttgca cctcatcaat
      541 cataaataaa atatcttttt cttggcacag gctttgcacg gcgtgcagat aatccgcctc
      601 taatgggcaa acaccgccct cgccctgcac cacctccagc aaaatcccgc aaacgttctc
      661 agtttccaag cgagctttca gtgcctcaat gccatttaca ggtaaatatt caaagccctc
      721 tgtaaagggg aaaaaatgct tgtggaatac ctcttgccca gtggatgcga gggttgaaat
      781 ggttcgtccg tgaaaagagt tcacgagggt taaaaccgtt gaacgaccgg aaccgtattt
      841 gtcgaaacta tatttgcggg cggttttaat tgctccttca tttgcctctg cccctgaatt
      901 gcaaaaaaat gcccgtttta gaccgcttgc agccactaat ttttgggcta attggcttgc
      961 cggttcggta aaaaacaggt ttgaggtgtg agctaatttg ccggcttgtg tggtaacagc
     1021 ttgtagccaa tcctcatccg cccagcctaa actatttacc ccaatgccac tggtaaaatc
     1081 aagatactta ttgccctcaa aatcccacac ttcacagcct tttccgtgcg acagtgcaag
     1141 gtcaaatctg ccataggttt gggcgatata atccttatct aattgcttaa tcgtattact
     1201 gcttgtcatt tcactttcct gttatttttt ataaaaccgt gtgccgtttt tgccaccgaa
     1261 catttctacc aaaatggcgt ggtcgattcg cccgtcaata atatttgcct cagagccacc
     1321 agctctgatg aaatcggtac agcacgcaat tttggggatc atcccaccgg caataatgcc
     1381 ttgctcaatc agggactgca cttcgcttac ttccacttcc ggaattaagg tgctttcatc
     1441 aaagcgatct cgcaataaac ctgcaatatc ggtcatcgag accaattttt ccgcttgtaa
     1501 cgcaatcgca atctcgctgg cagcggtgtc tgcattaatg ttataagcct gcccgttttc
     1561 atcaacgccc accgtggaaa caaccggaat caagcccgca ttaagagcaa tttcaatcac
     1621 agttggatcg actttcacaa tatcgcccac aaagccgtaa tccacctctg cctctaattt
     1681 tttgcattgc aacatcgccc catcaatacc acacagccca atgcctttcc cttttaataa
     1741 ggcgacaagg cttttattaa ctttgcctgc caacatttgc aaaaccacgt taatcgtatc
     1801 gctatcggta acacgcaacc cgttgataaa ttgcggttct ttacccaata atttcacgcc
     1861 ttgtgaaatc tccagcccgc caccgtgtac cagtaccact tttacgccta aacgatgaag
     1921 taacagcaga tctgccatca ctgatttttt cagttcctca ttaatcattg cgttaccgcc
     1981 atatttcacc acaatgattt taccctgcca tttcaccaaa caagcggtgg atttttctaa
     2041 taaatttgca aattgattga tttcttgttg ttgcatattt ctttctcttg tagggacggg
     2101 tcctgtgccc gtccgaatat gggcgatgat tatcggacag gcacaggacc tgtccctaca
     2161 tttatattta aagctctaac ccctcaacct catctcttcc caacataata ttcatacact
     2221 gcactgcggc acctgatgcc cctttgccta aattgtcaaa acgggcagca agtaaaagtt
     2281 gggtgctgtt gccgtaaata aaaatctcta agctgtcttt acctgcataa gcattcgcgg
     2341 caagcattcc gtcttcgggt agagattgga aagggtgaac gtgaattagt cggctggttt
     2401 ggtagtaatc ggcgagcaaa tttgcaattt cttcgccgga tttgaccgct tgtaaggcga
     2461 agatctgcaa tggcacagtg actaacattc cgctgtaata atcagcaacc acaggggtga
     2521 aaatcggtgg gttttgcatt tgggtaactg cctgcatttc aggcaaatgt ttgtgttgga
     2581 gagttaaacc gtaaacacga ggggcattga aagcatttgg gctgttttca ttttcatagt
     2641 cagcaatcat cgctttgcca ccgcctgaat agccggttaa ggaatgacaa cttaaagggt
     2701 aatcggcggg cagaatccct ttttccacca gtggacgaac caacgcaata tagcccgtgg
     2761 catggcaacc cggcaccgca acacgcttgg cattttggat tttttcacgt tgtccgctca
     2821 attccggaaa accataaacc caattaggat tgacccgatg agcggttgaa gtatcgcaaa
     2881 ttcgggtatt ttccggtgca tttgctacta attcttttga ggcttcatcc ggcaaacaga
     2941 ggaaagttaa atccgcctct gcgacttttg ccacacgggc gttcaaatct ttccgctgct
     3001 cttcgggcaa ggttaataat tcaatatcgt ccgcttggct taaacgttcg tgaatgcgta
     3061 gcccggttgt tcctactgcc ccatcaacaa aaatcttgta tttgctcatt gaaaatcctt
     3121 ctttgacttt ttctgctcgg tagtctaa
//
LOCUS       DANLXF010000246         3128 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.246, whole genome shotgun sequence.
ACCESSION   DANLXF010000246 DANLXF010000000
VERSION     DANLXF010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.246"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(74..1765)
                     /gene="dld"
                     /locus_tag="PXD66_002283"
     CDS             complement(74..1765)
                     /gene="dld"
                     /locus_tag="PXD66_002283"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDL6320532.1"
                     /translation="MSNNSLISQLTEIVGQSYIITDPTKTEAYRSGYRFGTGSAIAVV
                     KPTTLLEYWNVLKACVAADVIVISQAANTGLTGGSTPSGNDYDRDIVIINTMRMNGIQ
                     LINNANQVVCLPGSTLNELELLLKTHGREPHSVIGSSCIGASVIGGICNNSGGALVQR
                     GPAYTEMALFAQLNEDGTLELKNHLGIDLGDTPEEILENLQAQRYQRKDIQQACGKGH
                     DHAYCNHVRQIDEDSPARFNADPARHYEAAGSAGKLAVFAVRLDTFPLEKDTAVFYIG
                     TNHTDTLNDIRRYMLANFEVLPISGEYIHRDAFDIAADYGKDTFWVIKKLGTHRLPQL
                     FSLKAKVDRMAKKLTFLPANLSDKILQAISKMLPQHLPESLRDYRNQYEHHLIIKMGG
                     KGVQEARDYLARYFADTQKGGYLECNPTESQAAMLHRFAVASAAIRYRAIHDKEVEDI
                     VALDIALRRNDKAWFEVLPPEIESQITHKLYYGHFMCHVFHQDYIVKKGVDCLALEHQ
                     MLELLDKRGAQYPAEHNVGHLYEAKPTLRKFYRQLDPTNSFNPGIGQTSKKKYWK"
     gene            complement(1977..3074)
                     /locus_tag="PXD66_002284"
     CDS             complement(1977..3074)
                     /locus_tag="PXD66_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HDL6320533.1"
                     /translation="MFYSSNPLIKHKTGLLNLAEELGNISQACKVMGMSRDTFYRYQQ
                     AVEQGGVEALLNQNRRVPNIKNRVDESTEKAVVQFALDNPAFGQVRVSNELRKQGIFV
                     SAGGFCLCRWFLSLQVVFVSAGGVRSIWLRHHLANFKQRLTALEKLVAEQGIILSEPQ
                     IQALERKKEDEIACGEIETAHPGYLGSQDTFYVGNIKGVGRIYQQTFIDTYSKVAFAK
                     LYTMKTAITAADMLNDKVLPFFESQRLPMLRVLTDRGSEYCGKVENHDYELYLAINDI
                     EHTKTKVKQPQTNGICERFHKTILQEFYQVAFRKRIYMDLEALQADLDEWLMYYNHHR
                     THQGKMCCGRTPMETLVDGKRIWAEKNLSSN"
BASE COUNT          876 a          707 c          603 g          942 t
ORIGIN      
        1 attagcaact gttttgtcca cttggcatag tttttgacgg atcagcaact attttgtcta
       61 ctatgccttt ttgttacttc cagtattttt tcttcgaggt ttgcccgatc cccggattaa
      121 agctgttggt tggatcaagc tgccggtaga atttgcgtaa ggtcggtttt gcttcgtaaa
      181 gatgaccgac attatgctcc gcagggtatt gtgccccacg tttatccagt aattctaaca
      241 tctgatgttc caacgccaaa caatctacac cttttttcac aatatagtct tgatggaaaa
      301 catggcacat aaaatgccca taataaagtt tgtgcgtgat ttgactctct atttccggcg
      361 gtagcacttc aaaccacgct ttatcatttc ggcgaagggc aatatctaaa gcaacaatat
      421 cttccacctc tttatcgtga atggctcgat agcgaatcgc tgccgaagca acggcaaaac
      481 gatgtaacat tgccgcttgg ctttcagtcg gattacactc caaataaccg cctttttgtg
      541 tatcagcaaa ataacgggca agatagtctc ttgcttcttg aacgcctttt ccacccattt
      601 tgataattaa gtgatgttca tactggttac ggtagtctcg aagactttcc ggcaaatgtt
      661 gcggtaacat ttttgagatt gcctgtaata ttttatcact gagatttgcc ggtaaaaagg
      721 tgagtttctt cgccattctg tccacttttg ctttaagtga aaagagttgt ggtaagcggt
      781 gtgtaccgag ttttttaatc acccaaaaag tatctttacc gtaatcagcg gcaatatcaa
      841 aggcatcacg atgaatatat tcaccggaaa tcggcaatac ctcaaagttg gctaacatat
      901 agcgacgaat atcgtttaat gtgtccgtat gatttgtccc gatataaaaa acagccgtgt
      961 ctttttcaag cggaaaggta tctaatcgca ccgcaaacac cgctaacttt cctgcactac
     1021 cggcagcctc gtagtggcgg gcaggatcgg cattaaaacg ggcaggggag tcttcatcaa
     1081 tttggcggac gtgattacaa taggcgtgat cgtgtccttt tccgcacgct tgctgaatat
     1141 ccttacgctg ataacgttgg gcttgcagat tttctaaaat ttcttccggc gtatcaccta
     1201 aatcaatacc taaatggttt tttaattcca gcgtgccgtc ttcatttaac tgggcaaaca
     1261 gtgccatttc agtataagcc ggcccacgtt gcactaatgc accccctgaa ttattacaaa
     1321 taccaccgat aaccgaagcc ccgatacagg acgatccgat caccgaatgc ggttcacgtc
     1381 cgtgcgtttt cagcaacaat tccagctcat ttaatgtcga gcccggtaag caaacgactt
     1441 gattggcatt gttaattaac tgaatgccgt tcattcgcat tgtgttaatg atcacaatat
     1501 cccgatcata atcgttgccg cttggtgttg aaccaccggt taatccggta ttggccgctt
     1561 gtgagataac aatgacatcg gcggcgacac aggcttttaa tacgttccag tattctaata
     1621 acgttgtagg ttttaccact gcaatcgcac tacccgtgcc aaagcgatac ccgctacgat
     1681 aggcttccgt cttagtcggg tcagtgatga tataagactg cccgacaatt tcagtaagct
     1741 gagaaatcag tgaattattt gacattatgc ttccctcaaa ggtagaaata gatagggcag
     1801 tatagcaata aacaaaagag agcaaagcag aatgttacaa aattgtgaac tactgcacag
     1861 aataaatgaa gaaatgaagt aaaagaaagc gagctaaaag agcgtcgccc gctttatttg
     1921 tagaaaatca aacctaatct gacagtcccc cgtttaaaat taccgtgtct gtcagattaa
     1981 tttgagctta aattcttttc tgcccaaatg cgttttccat caactaatgt ctccatcggt
     2041 gttcttccac agcacatttt tccttgatgg gttcgatgat ggttataata cattaaccat
     2101 tcatctaagt cagcttgcaa tgcctctaaa tccatataaa tccgttttct gaatgccact
     2161 tggtagaact cctgcaaaat cgttttgtga aaacgctcac agatgccgtt tgtttgtggc
     2221 tgtttcactt tggtttttgt gtgctctatg tcattgatag ccaaataaag ctcataatcg
     2281 tgattctcaa ctttcccaca atactcactg cctcggtctg tcagcactcg taacataggt
     2341 aacctctggc tttcaaagaa tggtaatact ttgtcattta gcatatcagc agcagtgata
     2401 gccgtcttca ttgtatacag ttttgcaaat gccactttac tataagtatc aataaacgtt
     2461 tgctgataaa tacgacccac acctttaata ttgccgacat aaaaggtatc ttgtgaccct
     2521 aaatagccgg ggtgtgccgt ttcaatttca ccacaggcaa tctcatcttc tttcttccgc
     2581 tccaaggctt gtatctgtgg ctcactcaga ataatgcctt gctctgccac caatttttcg
     2641 agggcagtta agcgttgctt aaaattagct aagtgatgtc tcaaccaaat ggaacgtaca
     2701 ccacctgcag agacaaaaac cacctgcaga gacaaaaacc acctgcagag acaaaaacca
     2761 cctgcagaga caaaaatccc ctgtttacgc agttcattac tcactcgaac ttgaccaaaa
     2821 gcaggattat ctaaagcgaa ttgtacaacc gctttttctg tggattcatc cacacgattt
     2881 ttaatgttgg gaacacgtcg attttgattt agtaatgcct caacaccgcc ttgttcgaca
     2941 gcctgttgat agcgataaaa agtatctcga ctcatcccca ttaccttaca ggcttgtgag
     3001 atattaccca gttcttctgc taaatttaat aaaccggtct tgtgtttaat aagtggattg
     3061 ctagaataaa acatgagagt ttcctttttg tttagattga tttttgacac tcatattcta
     3121 aacgggaa
//
LOCUS       DANLXF010000247         3083 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.247, whole genome shotgun sequence.
ACCESSION   DANLXF010000247 DANLXF010000000
VERSION     DANLXF010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.247"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..1071
                     /locus_tag="PXD66_002285"
     CDS             <1..1071
                     /locus_tag="PXD66_002285"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDL6320534.1"
                     /translation="EGVKSLSPYQAGKPIEELERELGIQNIIKLASNENPFGFPESAK
                     KAILNQLDDLTRYPDSNGFELKTTIAQKFGVQANQITLGNGSNDLLELFAHTFAGEQD
                     EIIYSQYAFIVYPLVTKAINAVAREILAKNWGHNLDAFLAAITEKTKLIFIANPNNPT
                     GNFLTQAEIEGFLAKVPANVIVVLDEAYTEFTAPQERVDSFGLLAKYPNLIVSRSLSK
                     AYGLAGLRIGYAVSNPEIADLLNRVRQPFNCNSLALASAIAVMNDDEFVKKVAENNRL
                     EMARYEAFCQANGLEYIPSKGNFITIDFKRPAAPIYEALLREGVIVRSIAGYGMPNHL
                     RISIGLPQENERFFTALLKVLA"
     gene            1117..2415
                     /gene="aroA"
                     /locus_tag="PXD66_002286"
     CDS             1117..2415
                     /gene="aroA"
                     /locus_tag="PXD66_002286"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDL6320535.1"
                     /translation="MEKLTLTPISRVEGEINLPGSKSLSNRALLLAALATGTTQVTNL
                     LDSDDIRHMLNALKALGVKYELSDDKTVCVVEGIGGAFKVQNGLSLFLGNAGTAMRPL
                     AAALCLKGEEKSQIILTGEPRMKERPIKHLVDALRQVGAEVQYLENEGYPPLAISNSG
                     LQGGKVQIDGSISSQFLTALLMSAPLAEGDMEIEIIGDLVSKPYIDITLSMMNDFGIT
                     VENRDYKTFLVKGKQGYVAPQGNYLVEGDASSASYFLASGAIKGKVKVTGIGKKSIQG
                     DRLFADVLEKMGAKITWGEDFIQAEQSPLKGVDMDMNHIPDAAMTIATTALFAEGETV
                     IRNIYNWRVKETDRLTAMATELRKVGAEVEEGEDFIRIQPLALENFQHAEIETYNDHR
                     MAMCFSLIALSNTEVTILDPNCTAKTFPTYFRELEKLSVR"
     gene            complement(2469..3080)
                     /locus_tag="PXD66_002287"
     CDS             complement(2469..3080)
                     /locus_tag="PXD66_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDL6320536.1"
                     /translation="MKKLSSALLSCFLLGLSSPALTADTYITENLSTFMRKGAGDQFR
                     ISGAIQAGEKVTVLDRKERYSLIRDSRNREGWVLNSDLSDTASPKELIPQLKQQVQDL
                     TNRLSKIDTEWQQRTAEMQRRTQDSVQKSSDLVEENAQLKRELEILKNKNRDLETMHD
                     SEKREIVIQWFIYGGAVLGAGLLLGLLVPFLIPRRRRNNSGWA"
BASE COUNT          905 a          612 c          686 g          880 t
ORIGIN      
        1 gagggcgtga aatccctttc gccttaccaa gcaggaaagc cgattgagga gctagagcgt
       61 gaattaggga ttcaaaatat cattaaatta gcctcgaacg aaaacccatt tggttttcct
      121 gaaagtgcaa aaaaggcgat tctgaatcag cttgacgatt taacccgcta tccggattca
      181 aacggctttg aattaaaaac cactattgcc caaaaatttg gcgtacaagc caatcaaatc
      241 accttaggca atggctcaaa cgatttatta gagctgtttg cccacacctt cgccggtgag
      301 caagatgaaa ttatctattc tcagtatgcg tttattgtgt atccgttggt gacgaaagcg
      361 attaatgccg ttgcccgtga aattctggct aaaaattggg gacacaattt agatgccttt
      421 ttggctgcaa ttaccgaaaa aactaaatta atttttattg ccaacccgaa taatccgacc
      481 ggtaattttt taacccaagc ggaaattgag ggctttttag ctaaagtgcc ggcaaatgta
      541 atcgtggtgc tggacgaagc ttataccgag tttactgcgc ctcaagaacg ggtggattcg
      601 tttggcttat tggcaaaata cccgaattta attgtgtcac gttcactctc taaggcttac
      661 ggcttagccg ggcttcggat tggttatgcg gtttcaaacc ctgaaattgc ggatttatta
      721 aatcgtgttc gccagccgtt taactgcaat agtcttgcct tagcttcagc gattgcggta
      781 atgaatgatg atgaatttgt caaaaaagtg gcagaaaata accgcttgga aatggctcgc
      841 tatgaggctt tctgccaagc aaacggcttg gaatatatcc cttctaaggg caattttatt
      901 actattgatt tcaaacgtcc tgccgctccg atttatgagg cattactgcg tgaaggcgtg
      961 attgttcgct cgatagcagg ttatggaatg ccgaatcatt tacgcattag tatcggttta
     1021 ccgcaagaaa acgagagatt ttttactgcc ttattgaaag tgttagctta acaagcggtt
     1081 accttttatg aaaattttac aaatttaaag agaaaaatgg aaaaactaac tttaaccccg
     1141 atttcccgag tagaaggcga gatcaattta cctggttcta aaagcctgtc taaccgagcc
     1201 ttattattag ccgccttagc caccggtacg actcaagtga ccaatttatt agatagtgat
     1261 gatattcgac atatgctcaa tgccttaaaa gcgttaggcg tgaaatatga gctatcggac
     1321 gataaaaccg tctgtgtagt tgaagggatt ggtggagctt ttaaggttca aaacggctta
     1381 tcactgtttc tcggcaatgc aggcacggca atgcgaccac ttgcagcagc attgtgttta
     1441 aaaggtgagg aaaaatccca aatcattctt accggtgaac caagaatgaa agaacgcccg
     1501 attaaacact tagtcgatgc tttacgccaa gtaggggcag aggtacagta tttagaaaat
     1561 gaaggctatc caccgttggc aattagcaat agcggtttgc agggcggaaa agtgcaaatt
     1621 gacggctcga tttccagcca atttctaacc gcattgctga tgtctgcccc attagcggaa
     1681 ggcgatatgg aaattgagat tatcggtgat ctggtatcaa aaccttatat tgatattacc
     1741 ctttcgatga tgaacgattt tggtattacg gttgaaaatc gagattacaa aaccttttta
     1801 gttaaaggta aacaaggcta tgttgctcca caaggtaatt atttggtgga gggagatgcc
     1861 tcttctgcct cttatttctt agcctccggt gcgattaaag gcaaggtaaa agtaacgggc
     1921 attggtaaaa aatcgatcca aggcgaccgc ttgtttgccg atgtgttgga aaaaatgggg
     1981 gcaaaaatca cttggggaga ggattttatt caagccgagc aatccccgct aaaaggcgta
     2041 gatatggata tgaatcatat tcctgatgcg gcaatgacga ttgcaacaac cgctttattt
     2101 gccgaaggag aaacagttat ccgcaatatt tataactggc gggtaaaaga aaccgaccgc
     2161 ttgacagcaa tggcaaccga attgcgtaaa gtcggggcag aggtagaaga aggggaagat
     2221 tttattcgga ttcaaccgct tgcgttagaa aacttccagc acgctgaaat tgaaacctat
     2281 aacgatcacc gtatggcaat gtgtttttca ttaattgcgt tatcgaatac agaagtgacg
     2341 atcttagatc caaattgtac cgctaaaacg ttcccgactt actttaggga gttggaaaaa
     2401 ttatcggtca gataaaagta aaaaaggatt cagaaaactg aatccttttt acgttttatt
     2461 gtggcagact aagcccaacc gctgttgtta cgtctgcggc gagggatcag gaaaggcacg
     2521 agtaacccaa gtagtaaacc tgcgcctaac acagcaccac catagatgaa ccactgaatc
     2581 acgatttcac gtttctcgga atcgtgcatt gtttctaaat cacgattttt gtttttcagg
     2641 atttccagtt cacgttttag ttgtgcattt tcctcaacca aatcactgct tttctgcact
     2701 gaatcttgcg ttctacgttg catttctgcg gtacgttgtt gccattcggt atcgattttg
     2761 ctcaggcgat tggttaaatc ttgaacctgt tgttttaatt gaggaatcag ctcttttgga
     2821 ctagcagtat cgcttaagtc tgaattaagt acccaacctt cacggttacg actatctcga
     2881 atcagactgt agcgctcttt acgatctaat actgtgactt tttcacctgc ttgaatagcg
     2941 cctgaaatgc ggaattgatc accagcacct ttgcgcataa atgtactcag attttcagta
     3001 atgtaagtgt ctgccgttaa agcagggcta gataggccga gtaaaaaaca agacagaaga
     3061 gctgatgata atttcttcat ata
//
LOCUS       DANLXF010000248         3016 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.248, whole genome shotgun sequence.
ACCESSION   DANLXF010000248 DANLXF010000000
VERSION     DANLXF010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.248"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(73..1821)
                     /gene="dnaG"
                     /locus_tag="PXD66_002288"
     CDS             complement(73..1821)
                     /gene="dnaG"
                     /locus_tag="PXD66_002288"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDL6320537.1"
                     /translation="MKGTIPRSFIDDLIARTDIVEVINSRVKLKKAGRDYQACCPFHH
                     EKTPSFSVSQSKQFYHCFGCGVHGNVITFLMDYDKLEFPEAIEELAAMHGLEVPRENV
                     IQRDGKPQANFKTKRNLYELMEAVAKFYQQNLSHQIEPQSYLQQRGLSEEIIERFEIG
                     FAPNAFDAALRTFGQSQEDIQKLLDTGVLTKNDSGRIYDKFRNRVMLPIRDKRGRVIA
                     FGGRVLPNDEQGAKYMNSPETVIYHKGSELYGLYQALKVNETPDYLFVVEGYMDVVAL
                     AQFGVDNAVASLGTATTGEQIQQMFRCTEQIVCCYDGDRAGRDAAWRALENALPYLQD
                     GRQLKFIFLPDGEDPDTFVRSQGKEGFEQYVAQNAKPLSDFLFDSLLADVDLSSKEGK
                     SKLAALAVPLINRIPGEMLRVYLRNILGQKLGILDPTQIEALLPTKKAAEKPLHQAVK
                     MKRTPMRLLIALLIQNPYFVKFVPDVSALASLKDPGFTLLTDIVATCQQNAGISMGGL
                     LEHYREDPQYKIVEALANWDHLELPENIERTFIDTLDFLYAKLVELRKEELIAKDRTV
                     GLTDDEKKEYALLMFQ"
     gene            complement(1975..2190)
                     /gene="rpsU"
                     /locus_tag="PXD66_002289"
     CDS             complement(1975..2190)
                     /gene="rpsU"
                     /locus_tag="PXD66_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005701713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDL6320538.1"
                     /translation="MPVIKVRENESFDVALRRFKRSCEKAGILAEVRAREFYEKPTTI
                     RKREKASLAKRHAKRNFRENARNTRLY"
     gene            complement(2338..2934)
                     /locus_tag="PXD66_002290"
     CDS             complement(2338..2934)
                     /locus_tag="PXD66_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HDL6320539.1"
                     /translation="MSLLPMNEITSLNQLDLQKSYSYADYLLWKFKERVEIIKGKIVA
                     MSPAPTRFHQRISMKMTAKFLEVFDNHQCQIYSAPFDVRFPDENGKVKTVVQPDLCVI
                     CDVSKLDERGCVGAPDLVVEILSPGNTKREMKDKYELYQEQGVKEYWIVSPETRTIQI
                     FVLENGKYIGIQPVAEDEPATSVVFPALSFSTEKLFEL"
BASE COUNT          868 a          704 c          566 g          878 t
ORIGIN      
        1 tcgttcatca cttctgccaa atctccccta acccctcttt gctaaagagg ggaattattc
       61 gagcttaaaa ttctactgaa acatcaaaag agcatactct tttttctcat catcggttaa
      121 gcccacggtt cggtctttgg caattagctc ttctttgcgt aattccacta atttggcgta
      181 gagaaaatcg agagtgtcaa taaatgttcg ttcgatattt tcgggtaact ctaaatgatc
      241 ccaatttgcg agggcttcaa caatcttata ttggggatct tcccgataat gttcaagtag
      301 cccgcccata ctgatacctg cattttgctg acaagttgcg acaatatcgg ttaataatgt
      361 aaagccagga tctttcaagg aggctaaagc acttacatcg ggtacgaatt tcacaaaata
      421 cggattttga atcaataacg caatcaacag ccgcattggc gtacgtttca ttttgaccgc
      481 ttgatgtaaa ggcttctctg ccgctttttt cgtcggtaac agagcttcta tctgcgttgg
      541 gtctaaaatg cctaatttct gtcctaaaat attgcgtaaa tacactcgca gcatctcgcc
      601 cggaatacga ttaattaacg gcacagctaa agcagcgagc ttggattttc cctcttttga
      661 agagagatcc acatccgcca gcaaactgtc aaataagaaa tcgcttaatg gcttggcatt
      721 ctgtgccacg tactgctcaa atccttcttt accttgagaa cgaacaaagg tgtccgggtc
      781 ttcgccatcg ggcaagaaaa taaatttcag ttggcgaccg tcttgcaaat aaggcaacgc
      841 attttctaac gcccgccaag ctgcatctcg accggctcta tcgccatcat aacaacagac
      901 aatttgctcg gtgcaacgaa acatttgctg aatttgttca ccggtagttg ccgtgcctaa
      961 agaggcgact gcattgtcca cgccgaattg ggcaagggcg accacgtcca tatagccttc
     1021 caccacgaac agataatccg gcgtttcatt gactttcagt gcttgataca agccatataa
     1081 ctcactgcct ttatgataaa tcaccgtttc gggcgagttc atatatttcg ccccttgctc
     1141 atcattcggc aacacccgcc caccaaaggc aattacccgt ccacgcttat ctcgaatcgg
     1201 taacattacc cgattgcgaa atttatcgta aattcgaccg ctatcatttt tggttaaaac
     1261 gcccgtatcg agcaatttct gaatatcttc ttgtgattgc ccaaaagtgc gtagggctgc
     1321 atcaaaggca ttcggagcaa aaccaatttc aaagcgttcg ataatctctt cagacaaacc
     1381 tcgttgctgt aaatagcttt gtggctcaat ctggtgcgat aaattctgct ggtagaactt
     1441 cgccaccgct tccatcaatt cataaagatt acgcttagtt ttaaaattag cttgaggctt
     1501 gccgtcacgt tgaatcacat tctctctcgg tacttccagc ccgtgcatag ctgccagttc
     1561 ttcaatcgct tccggaaatt cgagtttgtc ataatccatc aaaaaggtga tgacattgcc
     1621 gtgtacgcca cagccgaaac aatggtaaaa ctgttttgat tggcttaccg aaaaggaagg
     1681 ggttttctcg tgatgaaaag gacagcaggc ttgataatct cgcccagcct ttttgagttt
     1741 gacccgactg ttgatgacct caacaatatc ggtgcgagcg ataagatcat caataaatga
     1801 acgtggaatt gtgcctttca ttgatttcct cctgctcttt gaatgataca agcggagcaa
     1861 ttttgcaaat tttttgcaaa aaacaaccgc ttgaaataga aacgttaaaa acaccaaaac
     1921 cgtgagccta acggaatcac ggtttcgata tagtttgcta accagcctaa ctaattagta
     1981 taaacgtgtg ttacgtgcgt tctcacggaa attacgttta gcgtgacgtt tagcaagtga
     2041 tgctttttca cgtttacgaa ttgtagttgg tttttcgtag aattcacgag cacgaacttc
     2101 agctaagata cccgcttttt cgcaagagcg tttgaaacga cgtaatgcta cgtcaaatga
     2161 ttcattttca cgaactttaa ttactggcat atgccatcac ctcagaaaaa tatgattaaa
     2221 ttaaaccgca cacaattttg gcggcataga acaaaaaagg tagtgcattc taccttaagc
     2281 actcacataa gtaaagtagt attgtggcat aaaaccgccg tattatgttg catttttcta
     2341 cagttcgaat aatttctcgg tcgaaaaact taatgccgga aagaccaccg aagttgccgg
     2401 ttcatcttct gccaccggct gaataccgat atatttaccg ttttccaaca caaaaatctg
     2461 aatagtgcgg gtttcgggag aaacaatcca atactctttc accccttgtt cctggtaaag
     2521 ctcgtattta tctttcatct cccttttggt attacccgga gaaaggattt ccaccactaa
     2581 atccggtgcc cctacacagc ctctttcatc taacttactg acatcacaaa ttacacaaag
     2641 atcaggctgc actacagttt tgacttttcc gttttcatcg ggaaaccgaa cgtcaaaagg
     2701 cgcagaataa atctggcatt ggtgattgtc aaaaacctct aaaaattttg ctgtcatctt
     2761 catagaaatt cgctgatgaa aacgagtagg agccggagac atcgccacaa ttttcccttt
     2821 gataatctca acccgctctt taaatttcca tagcaaataa tcagcgtaac tgtaactttt
     2881 ttgtaagtcc agttgattta agctggtaat ttcgttcatt ggtaataagc tcataccgcc
     2941 tcctttatca gaaatataaa gatattatgc cgaataatcc ctcaaattca acaaaacttt
     3001 cgctaaaaag agatag
//
LOCUS       DANLXF010000249         2980 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.249, whole genome shotgun sequence.
ACCESSION   DANLXF010000249 DANLXF010000000
VERSION     DANLXF010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.249"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..1269)
                     /locus_tag="PXD66_002291"
     CDS             complement(<1..1269)
                     /locus_tag="PXD66_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HDL6320540.1"
                     /translation="MQEAYDIQHLCTRKEDKSFDRKSARKEPKEILRHLIGFANADGG
                     LLVIGIEDNGEITGFNYQKAHQPEEFIHALHNLQKMPIPVTYQQIEVVNSRGLADFVL
                     LFEVTASSGRVITSHDDRVFLRSKDQTLELNFEQRLQLEYEKGQRAFEEQPIARADFT
                     DLDMDLLEQYRQKLHTDRSIEEILKARDLINKEGQITNACILLFGNNPTRYFPNARLR
                     FLRYEGKAPLTGRTFNVVKEFMLEEPIPKLLESAKAVLKSQLREFQRLDENGQFTKID
                     EYPEFAWLEGIVNAIAHRSYSNQGDCIRISMFDDRLEIFSPGGLPRPVTLENMYYTRF
                     SRNPKIARVLYEFGWVKELNEGVNRMAEEMNAYQLNTPSYFEPNENAVRLILENNIEH
                     RTLRALERIEEIFTQDIMATLNKNEVTILQY"
     gene            complement(1369..1773)
                     /locus_tag="PXD66_002292"
     CDS             complement(1369..1773)
                     /locus_tag="PXD66_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005557532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HDL6320541.1"
                     /translation="MHKFTFPIEQIAPLVPHSGNMILLDRITAFGEDFLTAETNIRPD
                     NPLIKHGKLATYASIEIMAQAGEPVRLGYLLGTRKLHLHRQEIPIGSQLQIQIKMSIQ
                     DATGFGVFDCQLIDLADNQVLLEGALNVFSPK"
     gene            complement(1766..2980)
                     /locus_tag="PXD66_002293"
                     /pseudo
     CDS             complement(1766..2980)
                     /locus_tag="PXD66_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005544283.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
BASE COUNT          787 a          694 c          652 g          847 t
ORIGIN      
        1 atattgcagg atagtaactt catttttatt taatgtcgcc ataatatcct gcgtaaaaat
       61 ttcttctatt cgttctaatg cccttaatgt ccgatgttca atattatttt ctaaaatcag
      121 tctgaccgca ttttcattgg gttcaaagta agaaggtgta ttaagttggt aagcgttcat
      181 ttcctccgcc atacgattaa caccttcatt cagctctttg acccaaccaa attcatacag
      241 cactcgtgca atcttcggat tgcgtgaaaa acgggtatag tacatatttt caagcgtcac
      301 agggcgaggt aatccacccg gactaaaaat ttccaaacgg tcatcaaaca tcgaaatgcg
      361 aatgcagtcc ccttgattac tgtaactacg gtgtgcaatc gcattgacaa taccttctaa
      421 ccaagcaaat tctggatact catcaatttt tgtgaattgc ccgttttcat ctaaacgttg
      481 gaactctcgt aattggcttt ttaatactgc cttagcactc tccaataact taggaatagg
      541 ctcttcaagc ataaattctt tgacgacatt aaaagttcta ccggtcagag gtgctttacc
      601 ttcataccgc aaaaaacgca aacgggcatt aggaaaataa cgtgtcgggt tattcccaaa
      661 tagcaaaata caggcattcg taatttgccc ttctttgtta attaaatctc gggctttcag
      721 aatctcttct attgagcgat ctgtgtgtaa tttctgtcga tactgttcta ataaatccat
      781 atccagatca gtaaaatcag cgcgtgctat cggttgctct tcaaaagcac gctgcccttt
      841 ttcgtactct aattgcaaac gttgttcaaa attgagttcc aaagtttgat cttttgagcg
      901 tagaaaaacc cgatcatcgt gagaggtaat caccctccca ctcgaagctg taacttcaaa
      961 taataagaca aaatccgcta aacctcgact atttaccacc tcaatttgct gataggtcac
     1021 cggtatcggc attttttgta aattgtgcag agcgtggata aattcttccg gctgatgggc
     1081 tttttggtag ttaaaaccgg taatttcgcc attatcttca ataccaataa ctaataaacc
     1141 gccgtctgca ttcgcaaaac caatcaaatg ccgcaaaatc tctttcggtt cttttctcgc
     1201 acttttgcga tcaaaggatt tatcttcttt gcgagtacaa agatgttgaa tgtcataggc
     1261 ctcttgcata acgaatttcc taccaactac taccaactac taccaactac taccaactac
     1321 tacttactac tacttactac tacttactac tacttactac taccaactct atttcggact
     1381 aaacacattc agcgcccctt ctaaaagcac ttggttgtcg gctaaatcga tcagttggca
     1441 gtcgaatacg ccgaagccgg tggcatcttg aatagacatt ttgatctgaa tttgtagctg
     1501 tgagccaatc gggatttctt ggcggtggag gtgcagtttg cgagtgccga gcaggtagcc
     1561 taagcgaact ggttcgccgg cttgtgccat aatttcaatg ctggcgtagg tggcaagttt
     1621 tccgtgtttg atcagcggat tgtcagggcg aatattggtt tcagcggtca aaaaatcctc
     1681 gccgaatgcg gtaatgcggt cgagtaggat catattgcca ctgtgaggaa ccaatggtgc
     1741 aatttgttcg atagggaagg tgaatttatg catcgctttc tcccaaaatc agtacgctgt
     1801 tattgccgcc aaaggcaaaa gagctgcttg cgccaatccg tctgcctgct gcccaacggc
     1861 tctgttcatc ggtgatcgca atgttcggca ggttttcgtc cgcttgttta tcccataatt
     1921 gcgcagggag cttgccggtc gggttgtgtt ggcgacagag cattccccac aaaattgccg
     1981 cttccaccgc accggcagcc caagggtatg cccggtgtag ggtttggtgg tggtgcaagc
     2041 ggtctgatta ccgaagattt ttgccacagc gaggctttcc atttgatcgt tatgaagcgt
     2101 ccctgtgccg tgcaggttga tccagccgat gtcggctgcg tctaaatcag cattgtgcag
     2161 ggcattttgg aaagcggaaa ttgccccttc cccttcgggg tgaggtgagg acatatggta
     2221 agcgtccgag ctggaaccat aacccagtaa ctgaatgcga tgttcgtcca gtggttcacg
     2281 gctcatcaca aacaccgctg cgccttcgcc gatgttaatg ccgttacggt tagcggaaaa
     2341 tgggttggtt tgttggtttg ataacacttc cagcgagcta aatccgctga tagttaaagg
     2401 cgaaagggta tccacaccac cgcaaatcac cgcatcgcac aagccggctt tcagtaaacg
     2461 ggcagcagaa attaacgctc ttgcctctga ggtgcaggcg gtggaaatgc cgtaactcag
     2521 ccccgttaag ccatacactt cggcaacgaa atcagacggc gcagagaaga gaatttgctg
     2581 ttgattaaac tgctcggtgg cataatcgcc ctgcacaccg gctttgaaga cgggaatatt
     2641 ttcatcggcg ccggtagtgg acgtgccgat gatcaccgca atccgctcac gcccgaattg
     2701 ctcgatacaa gcggtaattt tcggctcaat ttctgcaagg gcgtgccata gcagttggtt
     2761 attgcggctg cggtgttcaa tagatagttc aacaggaaaa tcacgcaatc tagcagagac
     2821 ttgccctaga aatttcggtg aactatttga aatattatgt cgtaaataag gctgtgaagt
     2881 cgtatctaaa ggagatactt tcgcattaat taaaacattc aaatgctcca cgatgccata
     2941 tccaatcgga ctggtgatgg cgggctctga taaatatagt
//
LOCUS       DANLXF010000250         2877 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.250, whole genome shotgun sequence.
ACCESSION   DANLXF010000250 DANLXF010000000
VERSION     DANLXF010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.250"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(344..1585)
                     /locus_tag="PXD66_002294"
     CDS             complement(344..1585)
                     /locus_tag="PXD66_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HDL6320542.1"
                     /translation="MMNKRIYVFADWETFSQPELVGVLSSDVVRGKENFRFSYDNEWL
                     SSPYVQQIDPNLYLYPGEQYSTDTQNFHIFLDSCPDRWGRLLMKRREAALASQENRKP
                     KLLIESDYLLGVHDMNRMGALRFKTELNGPFLDNNTRLAAPPLSSLRELEFAAIQLES
                     SDNVDDPDYLKWLHMLISPGSSLGGARPKASVIDELNQLWIAKFPSRYDDYDIALWEY
                     LAYRLAIQAKIEMATCRIERFNQEHHTFLTQRFDRHCGKRRHFTSALTQLGYYDGDYE
                     ASYLEIAQFLTESGSNTRIDLAQLWRRIVFNIAISNTDDHLRNHGFIYAQEGWILSPA
                     YDLNPVPLSQGLHLNITDSDNRLDYQLAFDVADFFQLSKQQAVQIYDEVLKEINNWQN
                     VAKEIGIPRQEQEQMKFAFNV"
     gene            complement(1585..1896)
                     /locus_tag="PXD66_002295"
     CDS             complement(1585..1896)
                     /locus_tag="PXD66_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL6320543.1"
                     /translation="MVIIMLSKTKSPTLFPKYQNALLQLGENIRLASKRRMFTQTLLA
                     QRTGLSRVTVRKIMNGDPTVAIGYYLMVLGVLGLEQDFTDVGKDDELGRKLQDIELLR
                     K"
     gene            complement(2251..2844)
                     /locus_tag="PXD66_002296"
     CDS             complement(2251..2844)
                     /locus_tag="PXD66_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320544.1"
                     /translation="MKLKALSTLIASSMLYSQSFAVPPEKTAEEPQPFFNKTESGYDL
                     VFNDQNYRLIETELNGEKIQFRAFERIVYVNNPIEPDYQTINLYVPEAYYNNGEINGY
                     QADTAPIFLPNSVGGYMPAKAASYDKKRRDGSPGTILVALSKGYVVASVGARGRTLQK
                     EGKYTGKAPSAIIDSKSAAHKACRFESDHWHHSLCKI"
BASE COUNT          849 a          559 c          525 g          944 t
ORIGIN      
        1 aaatgttagt ttttctgatg tctgccatct gctaggcatt atatatgcct agattagttt
       61 cctacatttg cgtttttaag gctacgcttc cttgcggata tggttgagaa aaaagtcaag
      121 tgttactaat ggttttataa gatgttggtt tgttctggtg tctgccaccg ctaggtcttt
      181 ataccctaga ttagtatcag gtctttctgg tgcctgccac cgctaggcct ttacatccta
      241 gattaatatc agattgacaa tgagccaaca actcattgcg ttattaaggt aacgctactt
      301 ttttagaaca tagactacga tgattggttc cacaagtcaa ttactataca ttaaatgcaa
      361 atttcatctg ctcttgctct tggcgtggaa taccaatttc ttttgcgaca ttttgccaat
      421 tattaatttc tttcagtact tcatcataaa tctgtacggc ttgttgctta gataattgaa
      481 aaaagtcagc aacatcaaag gcgagctgat aatccaatcg gttatcgctg tctgttatgt
      541 ttaaatgtaa tccttgagat agtggtactg ggtttaaatc gtatgcggga gataatatcc
      601 acccctcttg agcgtagata aatccgtggt tacgcaaatg atcatcagta ttcgaaatag
      661 caatattgaa tacaattctt ctccatagtt gagccaagtc aattcgagta tttgaaccac
      721 tttctgttaa aaattgagca atctcaaggt agcttgcctc atagtctccg tcataataac
      781 ccagctgggt taatgctgac gtaaaatggc gacgttttcc acaatgtcga tcaaatcttt
      841 gtgtcaaaaa agtatggtgt tcttggttaa agcgttcaat tctacaagta gccatttcaa
      901 ttttggcttg aatagccagt cgataagcta aatattccca aagagcaata tcataatcat
      961 cgtaccgact tggaaatttg gcaatccata gttgatttag ttcatctatt accgaagctt
     1021 ttggtctagc tccgcctagt gacgaccctg gtgatatcaa catatgaagc cattttaaat
     1081 aatccggatc gtccacatta tcactgcttt caagttgtat tgctgcaaat tctaattctc
     1141 ttaaggaaga cagtggtggt gctgcaagac gagtattatt gtccaaaaat ggaccattca
     1201 attcagtttt aaaacgtaaa gcccccattc tattcatatc gtgtacgcct aaaaggtaat
     1261 cactttctat tagtaactta ggtttacgat tctcttgact agctagagct gcctcccgac
     1321 gtttcattaa caatctaccc catctatcag ggcaagaatc taaaaagata tgaaaatttt
     1381 gtgtatctgt actgtattgc tctccagggt aaaggtataa gttaggatca atctgctgta
     1441 catagggtga acttaaccac tcgttatcgt agctaaaacg gaaattttct ttgcctctca
     1501 ctacatcaga agaaagcacc ccaactaatt caggctgtga aaaagtttcc caatctgcaa
     1561 acacataaat tcgtttattc atcattattt ccttaataat tcaatatctt gcaatttacg
     1621 tcctaactca tcatcctttc caacatcagt gaaatcttgt tctagaccga gaacacctaa
     1681 aaccattagg tagtatccaa tcgcaacagt tggatcacca ttcataattt ttctcactgt
     1741 tacacggcta agcccagttc tttgagccaa taatgtttga gtaaacattc tcctttttga
     1801 ggctagccta atattttctc caagttgaag caaggcattt tggtacttag gaaacagagt
     1861 tggtgatttt gttttactga gcattattat taccattttt atgttttttg gaaattataa
     1921 tatccattta tagcaattgc aagagcagtt atgtaaacag taaaataggt agagagggtt
     1981 tttcggaaaa aaatatctgg tgaagttttg actaaatttg aattaaaaga aaaaatagat
     2041 tttttcaaat aaaatcaatc taaaaaatga ttttcaggct ttacaaactt gaaagaatgt
     2101 gtaagatcta gaacgtctga aattcggacg gaaaatccca atggatgtat tggctggata
     2161 catcgtagta actgtgtatt tgcgaatatg atttgaacaa tcctacacaa acgattcaaa
     2221 attcaaatgc ttacagcaaa ctatgtaaaa ctaaatttta catagtgagt ggtgccagtg
     2281 gtcggactcg aaccgacacg ctttatgggc ggcggatttt gaatcaataa tggcagaagg
     2341 tgctttgccg gtgtattttc cctctttttg tagcgtgcgt cctcttgctc ccacacttgc
     2401 caccacatag cctttagata acgccaccaa aatcgtacct ggtgaaccgt cacgtctttt
     2461 tttatcataa cttgccgctt ttgccggcat atagcccccc actgaatttg gcaagaagat
     2521 cggggcggta tccgcttggt agccattgat ttcgccatta ttgtagtaag cttccggcac
     2581 atacagatta attgtttggt aatctggctc aatcggattg ttcacataca caatccgctc
     2641 aaaggcacgg aattggattt tctcgccatt taattccgtt tcaattaaac gatagttttg
     2701 atcattaaag actaaatcat acccagattc ggttttattg aaaaagggct gtggctcttc
     2761 agctgttttt tccggcggta cagcaaaact ctgactatat agcattgaag aggcaatgag
     2821 tgtagataag gcttttagct tcattttttg tcctttatga aatatgaatc ggtacaa
//
LOCUS       DANLXF010000251         2875 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.251, whole genome shotgun sequence.
ACCESSION   DANLXF010000251 DANLXF010000000
VERSION     DANLXF010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.251"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(83..2026)
                     /gene="ftsH"
                     /locus_tag="PXD66_002297"
     CDS             complement(83..2026)
                     /gene="ftsH"
                     /locus_tag="PXD66_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDL6320545.1"
                     /translation="MVKNILLWVVIAIVMMTAYEGFNSNFSGSSNTVPYSTFLSDIKE
                     NRLKGVDFKQNDDTILVTKNDGATYQTVMPMYDEYLMADLAKTNAVITGQPAERRGLL
                     SQILISWFPMLLLIGFYVFYMRQMQGGGGRGAMGFGKNKAKMLTADEVKTRFSDVAGC
                     DEAKEEVGEVVEFLRDPTKFQKLGGRIPKGILMVGPPGTGKTLLAKAIAGEARVPFFT
                     MAGSDFVEMFVGVGASRVRDLFDQAKKNAPCIIFIDEIDAVGRKRGGAGFSGGHDERE
                     QTLNQMLVEMDGFEGSEGVIIIAATNRADVLDDALTRPGRFDRQVTVDLPNVKGREQI
                     LKVHLKKVPLAEGVDPMQIARGTPGYSGAQLANLVNEAALFAARKNKRVVMMEDFEEA
                     RDKINMGPERRSNTMTEKEIINTAYHEAGHVIVGYLMPEHDPLNKVTIVPRGQALGFA
                     QFLPEGDRVSETFTKLESQLSTLFAGRIAEGLIFGEDKITTGASSDIHRATQIARAMV
                     TQWGFSKELGPIFYQNEDGMGAIKGVSEESQKLIDVEMRKIIDRNYQRAKQTLEENMD
                     ILHAMKDALLKYETLDSKQIDDLMARRPVGEPSGWTDTPTKDDNATPPSGEQAADVSS
                     TLEDNPNKDNNIGDSLADTKAEH"
     gene            complement(2147..2773)
                     /gene="rlmE"
                     /locus_tag="PXD66_002298"
     CDS             complement(2147..2773)
                     /gene="rlmE"
                     /locus_tag="PXD66_002298"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HDL6320546.1"
                     /translation="MGRKRSASSSRWLAEHFKDQFVQKAHKQKLRSRAYFKLDEIQQT
                     DRLFKPGMTVVDLGAAPGGWSQYVVTQIGSKGRVIACDILDMNPIVGVDFLQGDFREE
                     SVLNALLERVGEDKVNVVMSDMAPNFSGMPSVDIPRAMYLVELALDMCRQVLATKGSF
                     VVKVFQGEGFDEYLREIRSLFSVVKVRKPEASRDRSREVYIVATGYKG"
BASE COUNT          779 a          717 c          508 g          871 t
ORIGIN      
        1 taaaaaaatc tgcactctat attggtgttt acctttagag tacagatttc ttcttattct
       61 aactttctag caaacaatta gattaatgtt ccgctttggt atcggctaac gaatccccaa
      121 tgttattatc tttatttgga ttatcctcta aagttgaact tacatccgct gcctgttcac
      181 cacttggtgg agtggcgtta tcgtctttag ttggggtatc tgtccaacct gatggttcac
      241 ccaccggacg gcgtgccatt aaatcatcaa tttgcttgct gtctaaggtt tcgtatttca
      301 ataaggcatc tttcattgcg tgtaagatgt ccatattttc ttccaaagtt tgcttcgctc
      361 gttgatagtt gcggtcaatg atcttacgca tttccacatc aatcaatttt tgcgactctt
      421 ccgatacgcc cttaattgca cccattccat cttcattttg atagaaaatc gggcctaatt
      481 cttttgagaa gccccattga gtaaccattg ctcgtgcaat ttgggttgct cgatgaatat
      541 cagaggaagc ccctgtggta attttatctt caccaaaaat caagccctca gcaattcgcc
      601 ctgcaaatag ggtggaaagt tggctttcca atttggtaaa tgtttcgctt acacggtcac
      661 cttccggtaa gaattgagca aaacctaacg cctgaccacg aggcacaatt gtcactttat
      721 tcagcggatc gtgttccggc attaaatagc caacaataac gtgtccagct tcgtggtaag
      781 cagtattgat gatctctttt tccgtcattg tatttgaacg gcgttccggc cccatattga
      841 ttttgtcacg ggcttcttcg aaatcttcca tcattaccac acgtttattc ttacgggcag
      901 caaataaggc agcttcgttc accaagtttg ctaattgagc accagagtaa cccggcgtac
      961 cacgagcaat ttgcatcggg tcaactccct ctgcaagcgg tacttttttc aagtgaactt
     1021 tcaaaatttg ctcacgccct ttcacgttcg gcaaatcgac cgtgacttgg cggtcgaaac
     1081 gccccggacg agttaaggca tcatctaaca catcggcacg gttggttgca gcgatgataa
     1141 tcacaccttc agagccttca aagccgtcca tctcaactaa catttggttg agggtttgtt
     1201 cacgctcatc gtgtccaccg ctgaaacctg caccaccacg tttacgccct accgcatcaa
     1261 tttcatcaat aaaaataata caaggggcat ttttcttggc ttggtcgaat aaatcacgca
     1321 cccgtgaagc acccacacct acaaacattt ccacgaagtc ggaacctgcc attgtaaaga
     1381 atggcactcg tgcttcacct gcaatcgctt tcgccaataa ggttttaccc gtacccggag
     1441 ggcctaccat caagatccct tttggaatgc gcccacctaa tttttgaaat ttagttgggt
     1501 cacgcaaaaa ctcaacaact tcgcctactt cttctttggc ttcatcacag ccggctacat
     1561 cagaaaatct ggtcttaact tcgtctgcgg tcaacatttt ggctttattt ttcccaaagc
     1621 ccattgcacc acgtcctccg ccaccttgca tttgtcgcat ataaaacaca tagaaaccga
     1681 ttaacagcaa cattgggaac caagaaatca aaatttgcga gagcaagcca cgacgctcag
     1741 caggctgacc ggtaatcact gcattggttt ttgctaaatc tgccattaag tactcatcat
     1801 acatcggcat tacggtttgg taggttgcac catcattttt cgtcaccaaa atggtgtcat
     1861 cattctgttt gaaatcgacc ccttttaaac gattttcttt aatatcgctt aaaaaagtcg
     1921 aatatggcac tgtatttgag ctaccactga aattagaatt aaaaccttca taggctgtca
     1981 tcatcacaat ggcaatcacc acccaaagta aaatattttt aaccatatcg ttcaaggtta
     2041 atcccctatc tgctgaataa aaaactgttc aagattacta taaaagcggt cgaattttcc
     2101 aatttttttg caaaatgtta ataaaaaatg accgcttgct tgttttctag cctttataac
     2161 ctgtcgctac aatataaact tctcgagacc gatctcgaga cgcctccggt tttctgactt
     2221 ttaccacact aaagagcgag cggatttctc gaaggtattc atcaaatcct tcgccttgga
     2281 ataccttaac cacaaaactg cctttggttg ctaatacttg gcgacacata tccaatgcca
     2341 attccactaa atacattgca cgaggaatat caaccgacgg cataccgctg aaattgggag
     2401 ccatatccga catcaccaca ttcactttgt cttcgcctac tcgctcaagt aacgcattca
     2461 gcaccgattc ttcacggaag tcgccttgta agaaatccac gcctacaatc gggttcatat
     2521 ctaaaatatc acaagcgatt actctgccct tactgccgat ttgcgtcacc acatattgcg
     2581 accaaccgcc cggtgcggca ccgagatcca ccaccgtcat tcccggtttg aataaacggt
     2641 cggtttgttg aatttcatct aacttaaaat acgcccgtga acgcaatttt tgtttatgtg
     2701 ctttttgcac aaattgatct ttaaaatgtt ctgcaagcca acgtgatgaa ctcgcacttc
     2761 gttttctacc catttgttct tctatcttaa ttaattcaac gatttataat aagggttaaa
     2821 ttagcaaatt caagagatgt tctaaaattt agctcggctt attatgctta attaa
//
LOCUS       DANLXF010000252         2874 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.252, whole genome shotgun sequence.
ACCESSION   DANLXF010000252 DANLXF010000000
VERSION     DANLXF010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.252"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..172)
                     /locus_tag="PXD66_002299"
     CDS             complement(<1..172)
                     /locus_tag="PXD66_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014668079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMP-N-acetylneuraminate-beta-galactosamide-
                     alpha-2, 3-sialyltransferase"
                     /protein_id="HDL6320547.1"
                     /translation="MNLIICCTPLQVLIAEKIIEMHPNERFYGVMLSTVKNAKFDFYQ
                     ARLAQKCEQFFAM"
     gene            complement(169..1374)
                     /locus_tag="PXD66_002300"
     CDS             complement(169..1374)
                     /locus_tag="PXD66_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HDL6320548.1"
                     /translation="MKAVKDSVIYLIGELASKSIPFLLLPYFSRKLGVAGYGELSYYQ
                     TFAALFVIFIGLSQDGAVARYFYFYGKRSLNLVLSTGYLYTLCATLLILLGCWYFQSE
                     IIAYVAIGAMFQSFLNVQLSVRQCQKQALAYTTIQLMSAIFSAVITVAMMEYFKTDLV
                     EKRILAILMSNGLVFLIAYFLYAKRAKTKKFSLARHKTALLYLFGFGIPLILHHSSIF
                     LKGQLDRVFIYHKFSEEDLGLYAMGAQIAMILAIVVQALNKATLPYFYEALKQKKINV
                     SHIHKWALYALFIVPLPAIVMWFIPEELVVWILGSQFVGTKYYIILFLIATMLVVPYL
                     ILVNYLFFYGKNKLISLCSILSTIFYLVALFALTFSKIEYIPFASIIGAISIIPILYI
                     MTAKVGKTV"
     gene            complement(1384..2568)
                     /gene="neuC"
                     /locus_tag="PXD66_002301"
     CDS             complement(1384..2568)
                     /gene="neuC"
                     /locus_tag="PXD66_002301"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HDL6320549.1"
                     /translation="MKNIAYITGSRAEYGIVKRLLQKLAQDPTVNFSLVVTAMHLDPQ
                     YGNTVTVIEQDGFEIAARIPLTLNSENNQTIIHSMAECLDRFGEHFQQHRYDAVIVLG
                     DRYEMLAVATAAAMHNIPLIHLHGGEQTLGNYDEFIRHCITKMSKLHLTSTEEYRQRV
                     IQMGELPENVVNTGSLGAENSLDLVLPTQAELVERLGIPASPYFLVVFHPETLTDVSV
                     LEQIEALLNAFEQFKDKYQFVFIGSNSDTNSEQIYARFKAYTDENQFKFFTSVKPEEY
                     LALIKYSNGLIGNSSSGLIEAPSCGVGTLNIGNRQQGRVRDESVIDVESNEQAIIDGI
                     EKLISADFQHRLPSMKNPYFQDNAMEKAYNAIKIFLADSHHTQPKGFYDVAFSQAVKK
                     SE"
     gene            complement(2569..>2874)
                     /locus_tag="PXD66_002302"
     CDS             complement(2569..>2874)
                     /locus_tag="PXD66_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HDL6320550.1"
                     /translation="SDEKILADINHLIAQLTAIRGDINLYFLEITPTAFRVDRNNNDI
                     RLLNQYLKDNLAVKWISLDQAFSDKYGKLDLSYSDDGLHLNAKGYEKLTAILEKELN"
BASE COUNT          943 a          596 c          488 g          847 t
ORIGIN      
        1 gcatcgcaaa aaattgttcg cacttttggg ctaatctagc ttggtaaaaa tcaaatttcg
       61 cgttttttac cgtagaaagc atcaccccat agaatcgctc attagggtgc atttcaatga
      121 ttttttcagc aatcaacact tgcaagggcg tgcaacaaat aattaaattc atacggtttt
      181 tcctactttt gccgtcatta tataaagaat cggaataatg ctgattgcac caatgatgct
      241 ggcaaaagga atatattcaa ttttagaaaa agttaaagca aatagtgcaa ctaaataaaa
      301 aatagtagat aaaatcgagc aaagcgaaat tagcttattt ttgccataaa aaaacaaata
      361 attgactaaa atgaggtaag gcacaaccaa cattgtcgca attaaaaaga gaataatgta
      421 gtattttgtt cctacaaatt ggctacccag aatccatacc actaattctt ccggaataaa
      481 ccacatcaca atagcaggta atggtacaat aaataaggca tacaacgccc atttgtggat
      541 atggctgaca ttaatttttt tctgctttaa ggcttcataa aaataaggca gtgttgcttt
      601 atttaaggct tgaaccacaa tggctagaat catcgcaatt tgagccccca ttgcatataa
      661 acctaaatcc tcttcgctaa atttatggta aataaatact ctgtctaact gtccttttaa
      721 gaaaatactg gaatggtgca aaatcaacgg aataccaaag ccgaataaat acaataaagc
      781 agttttatgg cgggcaaggc taaatttctt cgtttttgct cttttagcat acaaaaagta
      841 agcgattaga aagactaagc cattactcat caaaatcgct aaaatccgct tttccactaa
      901 atcggtctta aaatactcca tcattgcaac ggtaataact gccgaaaaaa tcgccgacat
      961 taattgaatc gttgtataag caagtgcctg tttttgacac tgccttacac taagctgtac
     1021 attcaaaaac gactggaaca tcgccccaat cgctacataa gcaataattt cagactggaa
     1081 ataccagcaa cccaacagga taagcaaagt tgcacaaaga gtatataaat accctgtact
     1141 caagactaaa tttagtgaac gcttaccata aaaatagaaa tagcgagcaa ccgcaccgtc
     1201 ttggctaagc ccgataaaaa ttacaaataa agcagcaaag gtttggtagt aggaaagctc
     1261 cccataacct gccacgccga gtttgcgtga aaagtagggt aagagcaaga aaggaattga
     1321 ctttgacgcc aattccccaa ttaaataaat cacactgtct ttaactgctt tcatagttgc
     1381 aaattattct gattttttga ccgcttgcga gaaagcaaca tcataaaatc ctttcggttg
     1441 agtatggtgg ctgtctgcta agaatatttt gattgcattg taagcctttt ccatcgcgtt
     1501 gtcttgaaaa tatggatttt tcatactcgg taaacggtgt tggaaatcgg ctgaaatcaa
     1561 tttttcgata ccatctatga tagcctgttc attactctct acatcaatta cgctctcatc
     1621 ccgtactcgc ccttgctggc gattaccgat atttagcgta ccaacaccac aactcggtgc
     1681 ttcaatcaag cctgatgagc tattaccaat caacccattt gaatatttaa tcaacgctaa
     1741 atactcttcc ggtttgactg aggtgaaaaa cttaaattga ttttcatcgg tgtaagcctt
     1801 aaaacgtgca taaatttgct ctgaattggt gtctgaattt gagccgataa agacgaattg
     1861 gtatttgtct ttaaactgct caaaagcatt gagtaacgcc tcaatttgct ccaacacgga
     1921 aacatcggtt aaggtttccg ggtggaagac cactaaaaaa taaggcgatg ccggaatccc
     1981 taatctctcg actaattctg cttgggtcgg caacactaaa tccaaactat tctccgcccc
     2041 tagagaacca gtattcacca cattttcggg cagctcgccc atttgaatca cacgctgtct
     2101 atattcttca gttgaagtta aatgcaattt cgacattttg gtaatacaat ggcgaataaa
     2161 ttcatcgtaa ttacctaatg tttgctcacc accgtgcaga tgaatcagcg gaatattatg
     2221 catcgccgca gccgttgcca ccgccaacat ctcgtaacga tcgcctagca caatcaccgc
     2281 atcataacga tgttgctgga aatgttcgcc aaatcgatct aaacactctg ccattgaatg
     2341 aataatggtc tgattatttt cactgtttaa ggtaagcgga atacgagctg caatctcaaa
     2401 gccatcttgc tcaatgaccg tcaccgtatt gccatattgc ggatccaaat gcatcgcagt
     2461 aacaaccaga gagaaattca cagtcggatc ttgtgctaat ttttgtaata aacgctttac
     2521 aatcccgtat tcggcacgag atccggtaat ataggcaata tttttcattt agttgagctc
     2581 tttttccaaa atagcggtta atttttcata accttttgca tttaaatgta atccgtcatc
     2641 gctatagctt aaatccaatt tgccatattt atcgctaaat gcctgatcta agctaatcca
     2701 tttcaccgct aaattatctt taagatattg atttaacaag cgaatatcgt tgttattacg
     2761 atcgactcta aatgcagttg gggtaatttc caagaaatag agattaatat ctccacgaat
     2821 tgctgttaat tgtgcaatta aatgattaat atctgctaga attttttcat cact
//
LOCUS       DANLXF010000253         2874 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.253, whole genome shotgun sequence.
ACCESSION   DANLXF010000253 DANLXF010000000
VERSION     DANLXF010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.253"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..474
                     /locus_tag="PXD66_002303"
     CDS             <1..474
                     /locus_tag="PXD66_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320551.1"
                     /translation="YVVGELHDYEFEHQQVAELNNFLKSLIANKIKSVEANFEVKESK
                     KVEGSYRIILDGKSLTESERSTVMDTFHFNSAQNPSITYQAEGNLVKLANREELLQKF
                     RFKQALEANVRYYKMKSGLQNTAEGALSIVMLPVAVVAVLPFYMFWVGACATYGC"
     gene            485..970
                     /locus_tag="PXD66_002304"
     CDS             485..970
                     /locus_tag="PXD66_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HDL6320552.1"
                     /translation="MYSLYLKILSECDWCCDPKTLSTYQQEWLLHEGSLTQKLYDVAT
                     NFNVEVVQEKWIAKNSEKTTACNEDVWSREVLLKDGEKNWIFAQTLVPRSTIENIAQN
                     VLELGNQPIGLWLFSQNPTRTKLEWTQTENGLFARRGVYEIRGYSLEIRELFLETFPY
                     L"
     gene            complement(1008..1877)
                     /locus_tag="PXD66_002305"
     CDS             complement(1008..1877)
                     /locus_tag="PXD66_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDL6320553.1"
                     /translation="MKNINTQAVGWIGLGQMGEPMAKHLLNSGVNVGVYNRDPAKAEK
                     VVEAGGTLYPSVLELVKAYDVIFLMIADYAAVQQVLSDEVLAELNGKVVVNMSTISPS
                     QNQAVEALLAQHGAEFVEAPVSGSSKVAEAGKLLVLAAGKEETVELLKPLFAAFSTTT
                     FYYGEVGKAGGIKLMINSLLGIFIQAYGEALNFADQYGIPKEKVIEMISGSFMNSPIF
                     QAKVPMYQANEFPPAFMMKHMTKDFNLASEEIAKLGKSYPLIEQATKTYNQANDSGLS
                     EVDMAAIYQFLAK"
     gene            2007..2798
                     /locus_tag="PXD66_002306"
     CDS             2007..2798
                     /locus_tag="PXD66_002306"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDL6320554.1"
                     /translation="MKPTILLYDSGMGGLTIYDEIRKVLPHAHYLYCFDNAFFPYSEK
                     SEAVLIERAVKIVQKIAEKQPLDMVVVACNTASTVVLPALREHFNFPIVGTVPAIKPA
                     AAISETKVIGLLATKGTVARPYVDELIRKYAQDCTVEKIGTTDLVEIVEEKQRSGKVD
                     MNRLQKVVEAWQGKPELDTVILGCTHFPLVKAELQQLLPDVKYFVDPGVGIAKRVLSL
                     LEERKFQQKADFENQAFCTKQSSAFNIKQDVMQNWGFNNLSVIDI"
BASE COUNT          871 a          544 c          602 g          857 t
ORIGIN      
        1 tatgtggtgg gtgaactgca cgattatgaa tttgagcatc agcaagttgc ggaactgaat
       61 aattttctca aaagcctaat tgctaataaa atcaaatctg ttgaggcaaa cttcgaggtt
      121 aaggaatcca aaaaagtgga aggtagttat agaattattt tagatgggaa atcacttaca
      181 gaaagtgaaa gaagtacggt gatggatacc tttcatttta attcagctca aaatccatct
      241 attacttatc aggcggaagg gaacttggtg aaattagcca atagagaaga gcttttgcaa
      301 aaattccgat ttaaacaagc gcttgaggcg aatgtgcgtt attacaaaat gaaaagtggt
      361 cttcaaaata cggctgaagg agcgttaagt attgtaatgt tgcctgttgc tgtagtagct
      421 gttttaccat tctatatgtt ttgggttggt gcttgtgcca cttacggatg ttaaataact
      481 ttttatgtat tctctatacc tgaaaatttt atcggaatgc gattggtgtt gtgatccaaa
      541 aacactttct acctaccagc aagaatggct tctgcacgaa gggtcactta cccaaaaatt
      601 gtatgatgtc gcaaccaatt tcaacgtgga agtagtacaa gaaaagtgga ttgcaaaaaa
      661 cagtgaaaaa acgaccgctt gtaatgaaga tgtttggtcg cgagaagtgt tactgaaaga
      721 tggtgaaaaa aattggattt ttgcccaaac tcttgtgcca agatcaacaa tcgaaaatat
      781 cgctcaaaac gtgttggaat tgggcaatca gccgattggt ttatggctat tttctcaaaa
      841 tcctactcgc accaaattag aatggacaca aacagagaac ggtttattcg ctcggcgtgg
      901 ggtgtatgaa attagagggt attctctgga aattcgagag ctttttttag aaacctttcc
      961 ttatttataa aaaaatcggt tggtaatgac accaaccgat tttcatctta ttttgccaag
     1021 aactgataaa ttgcagccat atccacttcg ctcaagcctg aatcattggc ttgattgtag
     1081 gttttagtgg cttgctcaat aagcgggtaa gatttgccta atttcgcaat ttcttcgctt
     1141 gccaaattaa aatctttggt catatgtttc atcataaacg caggcggaaa ttcattagct
     1201 tggtacatcg gcacttttgc ttggaagatc gggctgttca taaacgagcc tgaaatcatt
     1261 tcgattactt tctctttcgg aatgccatat tggtcggcga agtttaacgc ctcgccatag
     1321 gcttgaatga agatgcccag tagcgagttg atcatcaatt taatcccacc ggctttaccc
     1381 acttcaccat aatagaaagt ggtggtacta aaggcagcga aaagcggttt gagcaactca
     1441 acggtttctt ctttaccggc agccagtacc aatagtttac ctgcttctgc cactttgctt
     1501 gaaccggaaa ccggagcttc aacaaattct gcaccgtgtt gggctagtag agcttctacc
     1561 gcctggtttt ggcttgggga aatggtgctc atattcacca ctactttgcc gtttaattca
     1621 gccaacactt catcgcttaa cacctgttgc acggcggcat agtctgcaat catcaagaaa
     1681 atcacatcgt aggctttcac caattccaac acagaaggat ataatgtgcc gcctgcttca
     1741 actacctttt ctgcttttgc tgggtcgcgg ttgtaaacgc ccacgttcac tccgctattt
     1801 agcaaatgtt ttgccatcgg ttcgcccatt tgccctaaac caatccaacc gactgcttgt
     1861 gtattaatgt ttttcattat ttttctcctt actgaataaa gcaaattgat tattgcgatt
     1921 ttccctcatc aagtcaaata gagagaactt gaatatagca atggaaaaga gtagcatttt
     1981 agcacttact tttttgaaca ataccaatga aaccaacaat tcttttatac gattccggaa
     2041 tgggcggatt gacgatttac gatgaaatcc gaaaagtgtt accgcacgct cactatttat
     2101 attgctttga taacgctttt ttcccatatt ccgagaagtc ggaagcagtt ttaattgagc
     2161 gagcggtcaa aattgtgcaa aaaattgcag aaaaacaacc gcttgatatg gttgttgttg
     2221 cttgtaatac ggcaagcaca gtggttctgc cggcattgcg tgagcatttt aattttccga
     2281 tagtaggaac tgtaccggca attaaaccgg cagcggcaat ttccgaaact aaagtcatcg
     2341 gcttgctcgc cactaaaggc acggttgctc gcccttatgt tgatgaattg ataagaaaat
     2401 atgctcagga ttgtacggta gaaaaaattg gcacaaccga tttggttgaa attgttgaag
     2461 aaaaacaacg aagcggtaaa gtggatatga accgactaca aaaagtggtc gaggcttggc
     2521 aaggtaagcc ggaattggat acggttatac tcggttgtac ccattttccc ttagtcaaag
     2581 ctgaattaca gcaattattg cccgatgtga aatattttgt tgatccgggt gtaggtattg
     2641 ccaaacgggt gctaagcctt ttggaagaac gcaaatttca acaaaaagcc gattttgaaa
     2701 atcaagcatt ctgcactaaa caaagttctg catttaatat caaacaagac gttatgcaga
     2761 attggggatt taataatctt tcggtgattg atatttaaac aaaacaatct gttttgatga
     2821 aaaaaggttc agttgagcaa aaactttaaa aaagggcttg cactcaattt taaa
//
LOCUS       DANLXF010000254         2831 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.255, whole genome shotgun sequence.
ACCESSION   DANLXF010000254 DANLXF010000000
VERSION     DANLXF010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.255"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            42..2036
                     /gene="malQ"
                     /locus_tag="PXD66_002307"
     CDS             42..2036
                     /gene="malQ"
                     /locus_tag="PXD66_002307"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005710277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HDL6320555.1"
                     /translation="MKEYASPSAFEKIERLLNTDNDLQKNNQILPLVKVITQPNAEQQ
                     AVEISLNFTNVAKWQIVLENGEVRNGEIKAEQSVLTLPADLPLGYHQLSISTENDEES
                     CSLIVTPPKAFQPKPLEERKKLWGAFLQLYTLRSDNNWGIGDFGDLKQFLANISDYGA
                     DFVGLNPIHALFPADPDAASPYSPSFRLWQNIIYIDVSAVEAFQQSEQAQQWFHTKET
                     QQQLAELRAKDFVDYSQVAQLKLKALQFAFEHFSQSSQQQSQTAFDAFIEQAGEPLKI
                     QATFDALHHWISSEFGEKGGWTNWAEEYQSYYSPKVAEFQISQATQIRFYMWLQFVAN
                     QQLAECNRFAQSLSMPIGFYRDLAVGVTQSGAETWADKALFVQEASIGAPPDMLAPNG
                     QSWGLSPMHPHILQSRGYQPFIDLVRANMKHCGALRIDHILGFARMWWVANGESAKEG
                     IYVRYPLEDLLSILALESQRHQCLVIAEALGIVPEGILDSLEQKGIFAYNVFYFEKAE
                     NAFKPLENYPYQAMTTLSTHDLPTIQGYWKGYDFELGEKFDTYPSKAVLRKLKEERSY
                     DRLNIRNAVEKVIALEPNEVGVTIKFTHQLQRYVAHTHSALFGTQPEDWLNMLEPVNI
                     PGTSTEYPNWRRKLSATTEQIFANKDIQQLLKDINEIRKG"
     gene            complement(2104..>2831)
                     /locus_tag="PXD66_002308"
     CDS             complement(2104..>2831)
                     /locus_tag="PXD66_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HDL6320556.1"
                     /translation="CSSFSTKLKQEFFSGWTTFEASWLIVFLAIQIGIFIYQPDTWIA
                     TIAAITGILCVVFVGKGKISNYLFGLISVSLYAYISYTFQLYGEMMLNLLVYVPVQFI
                     GFYFWRKNMTSENTVNNAGVEEVIAKALTAKQWVIVAITTIIGTFLYIELLKYLGSAL
                     AILDGATVVISIVAQILMVLRYREQWALWIIVNIMTISLWTAMYFQNGETSLPLLVMY
                     VMYLCNSIYGYYNWIKLHRKHQQ"
BASE COUNT          900 a          603 c          564 g          764 t
ORIGIN      
        1 caaacaaaca aaatacagct cactattttt actccccaaa tattaaagag tatgccagcc
       61 cctctgcttt tgagaaaata gagcggcttt taaataccga taacgatttg caaaaaaata
      121 atcaaattct accgcttgtt aaagtgatta ctcagccaaa tgccgagcaa caagcggtcg
      181 aaatttcgct gaattttaca aatgtagcaa agtggcaaat tgtgcttgaa aatggtgagg
      241 ttcggaacgg tgaaattaag gcagagcaat cagttctaac cctgcctgcc gatttgcctt
      301 taggctatca tcagctttct atttcaactg aaaatgatga agaatcttgc tcgctgattg
      361 taacaccgcc gaaagctttc cagcctaagc cattggaaga aaggaaaaag ctctggggtg
      421 catttttaca gctttatact ttgcgttccg ataacaactg gggaattggc gattttggcg
      481 atctcaaaca attccttgcc aacatttctg attatggtgc agattttgtg ggcttaaacc
      541 ctattcacgc cctctttccg gcagatcctg atgcggcaag cccttacagc ccgtcctttc
      601 gactttggca aaatattatt tatattgatg tttcagcagt agaagccttt caacaatctg
      661 aacaggctca acaatggttt cacaccaaag aaactcaaca gcaacttgcc gaattgcgag
      721 ccaaagattt tgtcgattac agccaagtag cgcaactgaa attaaaggct ctgcagtttg
      781 cttttgagca tttttcacag tcatctcaac aacaatcgca aacagcgttc gatgccttta
      841 ttgagcaagc aggcgagcca ttaaaaatcc aagcaacgtt cgatgctttg caccactgga
      901 tttcatctga atttggcgaa aaaggcggct ggacaaactg ggcggaggaa tatcaaagtt
      961 actattcgcc taaagtagct gaatttcaaa tatcgcaggc aacgcagata cgtttttata
     1021 tgtggttgca atttgtggca aaccagcaat tagcagaatg caaccggttt gctcaatcac
     1081 tttcaatgcc gattggtttt taccgcgatt tagccgttgg tgttactcaa agcggagcgg
     1141 aaacttgggc ggataaagcc ctatttgtgc aagaggcgtc aatcggtgca ccacctgata
     1201 tgcttgcccc gaacggacag agctggggac tttcgccaat gcacccgcat attctacaaa
     1261 gccgaggtta tcagccgttt attgatttag tgcgtgctaa tatgaaacat tgtggggcat
     1321 tgcgaatcga ccatattctc ggcttcgccc gtatgtggtg ggtggcgaac ggtgaatcgg
     1381 caaaagaagg gatttatgtc cgttatccgc tcgaagattt gctctctatt ttggcattag
     1441 aaagccaacg tcaccaatgt ttagtgattg cagaggcact tggcattgtg cctgaaggca
     1501 ttttggactc tctcgagcaa aagggcattt ttgcctacaa tgttttttat tttgaaaagg
     1561 cagaaaacgc gtttaaaccg ctggaaaatt atccttacca agcgatgaca actctaagca
     1621 cccacgattt accgactatt caaggctatt ggaaaggcta tgattttgag ctaggcgaaa
     1681 aatttgacac ttacccaagc aaagccgtat tgagaaagct gaaagaagag cgttcgtacg
     1741 accgcttgaa tatccgtaat gcggtggaaa aagtgatagc actcgagcca aatgaagtgg
     1801 gagtaacaat aaaatttacc caccaattac aacgctatgt ggctcacact cacagtgcat
     1861 tattcggtac acagcccgaa gattggctca atatgttaga gccggtgaac attccgggaa
     1921 ccagcaccga ataccctaac tggagaagaa aattatccgc aaccaccgag cagatttttg
     1981 caaataaaga tattcaacaa ctgctaaaag atataaacga aattcgtaaa ggataataac
     2041 aagcggtcat ttttaggtaa atttttacaa aaaatgaccg cttgtaattt gtatttattc
     2101 gctctattgc tgatgttttc tgtgaagttt aatccagttg taataaccgt aaatagagtt
     2161 acataaatac atcacataca tcaccaacag cggcaagctg gtttcgccgt tttggaagta
     2221 cattgccgtc cataaagaaa tggtcataat attcacgata atccacaatg cccattgctc
     2281 acggtagcga agtaccatca aaatttgtgc cacaatcgaa ataaccactg ttgcaccatc
     2341 taatatagct aacgcactgc ctaaatattt aagcaattca atataaagaa atgtgccgat
     2401 aatggtggta atggcgacaa tcacccactg ttttgcggtt aaagctttag caattacctc
     2461 ttccacacca gcattattta ccgtgttttc gctggtcata tttttacgcc agaaatagaa
     2521 accgataaac tgcaccggca catatacaag taaattcagc atcatttcac catataactg
     2581 aaaggtataa gagatataag cataaagcga aacggaaatt aagccgaata gatagttact
     2641 gatttttcct ttaccgacaa ataccacgca taaaatacct gttattgccg caatagttgc
     2701 aatccaagta tcgggttgat aaataaaaat accgatttga atcgctaaaa aaacaatcaa
     2761 ccaacttgcc tcaaaagtag tccaaccact aaaaaactct tgttttaact tagttgaaaa
     2821 agatgaacag c
//
LOCUS       DANLXF010000255         2825 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.256, whole genome shotgun sequence.
ACCESSION   DANLXF010000255 DANLXF010000000
VERSION     DANLXF010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.256"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            28..717
                     /locus_tag="PXD66_002309"
     CDS             28..717
                     /locus_tag="PXD66_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HDL6320557.1"
                     /translation="MLQLRKANERGDGSFSWLKSYHTFSFANYYDLKHIHFSHLRVIN
                     EDFIAPNHGFGKHPHDNMEILTYMLKGRVAHEDSMGNKTEVKAGEFQIMSAGTGVFHS
                     EINPDPNETLHLYQIWIIPNQKDVTPRYNQDRFAPKEGATLILSPNAEEGSFKIYQDM
                     KLWRYQYPTAKSEQLALEPNRSYWLQVVKGNLNINGVELSTSDALGIRQEKALEINTQ
                     DDVEFLLFDLV"
     gene            complement(785..1576)
                     /locus_tag="PXD66_002310"
     CDS             complement(785..1576)
                     /locus_tag="PXD66_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HDL6320558.1"
                     /translation="MNNLRIKMISPEIALAKYNELDVLEINHSKVKAKIALQGAQLLS
                     WQPKNTQQDVLWLSEIEPFQTGVAIRGGIPICHPWFGTDKKPAHGTARIQAWNLLEHT
                     SSADGIRVVLGLENQAKLEMILGEVCELYFTHLAPEPAQAALHTYFQVGDITKTEVQG
                     LATQCFDKLTNQEIEVPSPRKISENVDCIYPAQAVNFIQDFANQRTIKVEHINATETV
                     LWNPWHNTVSAMTETAYQNMVCVETARINTLLQQNETLGVRILVR"
     gene            1700..2704
                     /gene="fbp"
                     /locus_tag="PXD66_002311"
     CDS             1700..2704
                     /gene="fbp"
                     /locus_tag="PXD66_002311"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HDL6320559.1"
                     /translation="MKTLGEFIIEKQAEYPGAKGELSGILSSIRLAAKIIHREINRAG
                     LSQDILGTAGSENVQGEAQMKLDVFANETMKKALLAREDVAGFASEEDDNFVAFDNER
                     GRNAKYILMTDPLDGSSNIEVNVSVGTIFSIYRRVSPIGTPVTQEDFLQEGRKQVAAG
                     YVTYGSSTMLVYTTGNGVNGFTYDPSLGLFILSHPDLKTPTEGKYYSINEGQYVTFPQ
                     GVKRFIKYCQETDEAIKRPYSSRYIGSLVSDFHRNLLKGGIYIYPTSTVYPKGKLRLL
                     YECNPIAFLAEQAGGMATDGINPILDIKPTELHQRVPFFVGSTSMVKQAGEFMQEFAK
                     "
BASE COUNT          874 a          597 c          537 g          817 t
ORIGIN      
        1 acttaacact tacataagga atagataatg ttacaactga gaaaagccaa tgaacgaggc
       61 gatggttctt ttagctggct taaaagctac cacacttttt cctttgccaa ttattacgac
      121 ctaaaacata tccatttttc acatttacgg gtaattaacg aagatttcat tgcacctaat
      181 cacggttttg gcaagcaccc gcacgataat atggaaattt taacttatat gttaaaagga
      241 cgtgtggctc acgaagacag tatgggtaat aaaaccgaag taaaagctgg cgaatttcaa
      301 attatgtctg ccggaaccgg ggttttccat tcagaaatta atccagatcc gaatgaaacc
      361 ttacaccttt accagatttg gattatccca aaccaaaaag atgtgacccc tcgctacaac
      421 caagatagat ttgccccgaa agagggagca accttgattc tttcgccaaa cgcagaagaa
      481 ggttcattta aaatttacca agatatgaag ctgtggcgtt atcaataccc aacagccaaa
      541 tcagagcaac tggcattaga gccaaaccgc agttactggc tacaagtggt gaaagggaat
      601 ctaaacatca atggcgttga actttccacc agcgatgcct taggcattcg tcaagagaaa
      661 gcacttgaaa taaacactca agatgacgtg gaatttttac tgtttgatct agtgtaaata
      721 caagagacaa gcggttaaat ttgtaaaaaa tattactaat tcagctgctt gctctcatta
      781 aacattatcg caccaaaatt ctcactccta gcgtttcatt ttgttgaagt aaggtattta
      841 tccgtgctgt ttccacacat accatatttt gatacgccgt ttctgtcatc gcactaacgg
      901 tattatgcca aggattccaa agtacggttt cggtagcatt aatatgctca actttaatgg
      961 tgcgctgatt tgcaaaatct tgaataaaat tcaccgcttg tgccggataa atacaatcca
     1021 cattttcact tatcttgcga ggtgatggca cttcaatttc ttgatttgtc aatttatcaa
     1081 aacattgtgt tgctaagcct tgtacttccg ttttcgtaat atcaccgacc tgaaaataag
     1141 tatgcaatgc agcctgtgcc ggctccggtg ctaagtgagt aaaatacaac tcacagactt
     1201 cgcctagtat catctcgagt tttgcctgat tttctaaccc caaaacaaca cgaatcccat
     1261 ctgctgagga agtatgttcc agcaaattcc acgcttgaat acgggctgtg ccgtgagcag
     1321 gctttttgtc tgtaccaaac caagggtggc aaatcggaat gccgccacga atcgccacac
     1381 ctgtttggaa aggttcaatt tcacttaacc ataatacatc ttgctgagta tttttaggtt
     1441 gccaactcag taactgcgca ccttgcaatg caattttagc cttcactttg gagtgattaa
     1501 tctctaaaac atccaattca ttatattttg ctaatgctat ttcagggctg atcattttga
     1561 ttcgtagatt attcatcgta ttgctcctca tcttgaaaga taaaatgtga agtggatcac
     1621 agaattaaag cttaaaccgc tacctcttta aaggtaatcg ttataataag ggcattctaa
     1681 catagcgaag gagaaaaaaa tgaaaacact cggcgaattt attattgaaa aacaagcaga
     1741 atacccaggg gcaaaagggg aattaagtgg gattttatct tctattcgtt tagccgctaa
     1801 aattattcat cgagaaatca accgtgccgg tttaagtcaa gatattttag gtactgcagg
     1861 ttctgaaaat gtacagggtg aagcccaaat gaaacttgat gtattcgcca acgaaacgat
     1921 gaaaaaagcg ttacttgcac gtgaagatgt tgccggcttt gcatcggaag aagatgataa
     1981 ttttgttgcc tttgataatg agcgtgggag aaatgcaaaa tatattttaa tgaccgatcc
     2041 gcttgatggc tcctccaata ttgaagtgaa tgtttctgtg ggaacaatct tctctattta
     2101 ccgccgtgtc tcccctatcg gcaccccagt aacacaagaa gatttcttgc aggaagggcg
     2161 taaacaagtt gccgccggtt atgttactta tggttcttca accatgctgg tttacaccac
     2221 aggcaatggc gtgaacggtt ttacctatga tccgtcatta ggcttattta ttctttcaca
     2281 ccctgatcta aaaaccccta ccgaaggaaa atattactcc atcaatgaag ggcagtatgt
     2341 tactttccca caaggtgtga aacgctttat taagtattgc caagaaaccg atgaagccat
     2401 caaacgtcca tactcttcac gctatatcgg ctcgctggtg tccgatttcc accgcaattt
     2461 attaaaaggc ggtatttata tttacccaac ctccaccgta tatccaaaag ggaaattgcg
     2521 tttactctat gaatgcaacc cgattgcgtt tcttgccgaa caagccggag gaatggcgac
     2581 agatggtatt aatccgattt tagatattaa gccaacagag ctacaccagc gtgtaccgtt
     2641 ctttgtcggt tcaacctcaa tggtcaaaca agcgggcgaa tttatgcaag aatttgcaaa
     2701 ataatgctcc tgcttatcac tcaataaaac tttcaacaaa gcagctgcgt attccgtagc
     2761 tgcttttgcc tatcttaaga ttgtaaattt gtataaaaaa caggtaagac ctcttgccaa
     2821 ataaa
//
LOCUS       DANLXF010000256         2795 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.257, whole genome shotgun sequence.
ACCESSION   DANLXF010000256 DANLXF010000000
VERSION     DANLXF010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.257"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            6..584
                     /locus_tag="PXD66_002312"
     CDS             6..584
                     /locus_tag="PXD66_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320560.1"
                     /translation="MIEFDQIDDSFYHFPFRPRPGDTSSDTATPCIGGVDLVSNPEKV
                     ELIPEAAHSPMLKKLLTDLNTPNSKLLTLGCAYWAHKDNRDTSYAYLEFSFREHSVAT
                     NLEFIRSIDEQFEQFLQENKKQLSIEFSVPEQAFDIVSQALFWSIRPFSYFGSEERIL
                     IYFQAGSPRHQDLEIFLDLLHRFLTEYLVVPA"
     gene            complement(599..674)
                     /locus_tag="PXD66_002313"
     tRNA            complement(599..674)
                     /locus_tag="PXD66_002313"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(639..641),aa:Trp,seq:cca)
     gene            1101..1295
                     /locus_tag="PXD66_002314"
     CDS             1101..1295
                     /locus_tag="PXD66_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005714695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320561.1"
                     /translation="MALSRTEIVNRSNAKRGIKLKAFKLHESTIAQIEQLAQQLNIPQ
                     NQLIKLAVEKLQEQTNFTTG"
     gene            complement(1263..1628)
                     /locus_tag="PXD66_002315"
     CDS             complement(1263..1628)
                     /locus_tag="PXD66_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320562.1"
                     /translation="MQIDVKGVLNLWGGYVRFTDCKGYPTMQAFMRESPQERTKQRYL
                     ARLDDDTLEKVDRQMRKLQANDKRQFDVLFYKYAVGLENKQVWQKLSLSKTSYHTELL
                     LAERFMEGALAGSEIRLLL"
     gene            complement(1618..1986)
                     /locus_tag="PXD66_002316"
     CDS             complement(1618..1986)
                     /locus_tag="PXD66_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDL6320563.1"
                     /translation="MSDWLEIALPYPPSVNHYWKHTRNGRHYISDKGKAFRAEVVKIC
                     KMFDPFKGAVQTVLQIYYPDKRKRDPDNLEKALWDALKIGGLIEEDDNQILVDKRTIT
                     AGFKKGGMVVLKIKAVGNAN"
     gene            complement(1983..2267)
                     /locus_tag="PXD66_002317"
     CDS             complement(1983..2267)
                     /locus_tag="PXD66_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HDL6320564.1"
                     /translation="MANLRKEAKGRECQVRLPGICNHNPETVVLAHYRMAGLNGVGMK
                     PDDIFGAWACSSCHDECDRRTRKLEADYVRQAHAEGVFRTQAILRREGKL"
BASE COUNT          879 a          655 c          444 g          817 t
ORIGIN      
        1 taaaaatgat tgaatttgat caaattgacg atagtttcta tcacttccca tttagaccac
       61 gtccgggcga cacttcaagc gacactgcca caccttgtat tggtggagta gatcttgttt
      121 ccaatcctga aaaggtagaa ctaataccag aagccgcaca ctccccaatg ttaaaaaagt
      181 tactcacaga cttgaacact ccaaacagca aacttctaac actaggctgt gcttattggg
      241 ctcataaaga caatagagat acatcatacg cttacttaga gttttcattc cgggaacatt
      301 cagttgcaac taacctagaa tttatcagaa gtattgatga acagttcgag caattcttgc
      361 aagaaaataa aaagcagtta tctattgaat tttccgtgcc tgagcaagca tttgatatag
      421 tttcacaagc tcttttctgg agtattcgtc cattttctta cttcggaagc gaagagcgca
      481 tcttgatata ctttcaggct ggtagtcctc gacatcagga tttagaaatc tttttggatt
      541 tactacaccg ctttttgact gagtatttag ttgttccagc ataacattta ctcgcatttg
      601 gaaagggtgg ttggactcaa accaacaacc aacgattttg gagaccgttg ctctacctat
      661 tgagctacac ccttaaaatc cgtaaacaaa aaagccccaa gcatttctgc tcagggctgt
      721 aaaattcttt taagttcacc acctatgcaa tgaaccgcaa cttaccataa ataatataga
      781 ttaagtccga actttgcaac actttttaat catttaaatg caaaaaagcc cataatttac
      841 aaaaccatgg gctttttaac gatattttgc ttgaatatat tggttatcca acatataata
      901 aacttgtttt cagtaatagt gctggaaacg agccggtggc gacctttaat cccaccgact
      961 tttggaggga taacaaatgc tttaccgcat tatcctagtt atcattctct tagtgattag
     1021 cctgccagca tactaattga atgaaactaa gtagggggag aaatctccct actcttcaaa
     1081 acctatatta aggatttatg atggcactgt caagaaccga aattgtaaac cgtagcaacg
     1141 caaaacgggg aattaaactt aaagccttta aactgcacga aagcacaatc gcacaaatcg
     1201 agcaacttgc ccaacagtta aatattcccc aaaatcaact aattaaatta gccgttgaga
     1261 agctacaaga gcaaacgaat ttcactaccg gctaatgccc cctccataaa tcgttcagca
     1321 agaagtaatt ctgtatgata tgaagtcttg ctgagcgata acttctgcca aacctgctta
     1381 ttctctaacc ctactgcata tttataaaat agtacatcga attgtcgctt gtcgttcgct
     1441 tgtagcttcc gcatttgccg atcgactttc tcaagcgtat catcatctaa gcgggctaaa
     1501 taacgctgtt ttgttctctc ttgtggagac tcacgcataa atgcctgcat tgtcggatag
     1561 cccttgcagt cggtaaaacg cacatatcca ccccataaat ttaatacacc ctttacatca
     1621 atttgcatta cccaccgcct taatctttaa caccaccatt ccccctttct taaaacctgc
     1681 cgtaatggtt cgcttatcga ctaaaatttg attatcgtct tcttcaatta agccaccgat
     1741 ttttaatgcg tcccacaagg ctttttctaa attatctgga tcacgcttgc gtttatcagg
     1801 atagtaaatc tgcaataccg tttgtaccgc tcctttaaac ggatcaaaca ttttgcagat
     1861 tttcaccact tccgccctaa aggctttccc cttatctgaa atataatgcc taccgtttcg
     1921 ggtatgcttc cagtaatggt tcacactcgg cggataaggc aacgcaattt caagccaatc
     1981 actcataatt tcccttctct ccgtaaaata gcctgcgttc taaatacgcc ctctgcatga
     2041 gcctgtctca cataatccgc ttctaatttg cgagttctac ggtcgcactc atcgtggcag
     2101 ctactgcaag cccacgcccc gaaaatatca tcgggtttca ttcccacacc gtttaatcct
     2161 gccattcgat agtgagccaa taccaccgtt tccggattat gattacagat acccggtaaa
     2221 cggacttggc actctctgcc ttttgcctct ttgcgtagat tagccattcc aacctccttg
     2281 gtctctttta tcaaaccact caataaaaaa gagtgaaccg gcacatacac taagaatagc
     2341 gatagtgatt aaaattacag tcagttcatt gcactggctc acttattaat ctccttaaat
     2401 taaataacat tccacataaa aggaataatc cgcaaccgat aacaaacgat attaaaatcc
     2461 aaaaccctaa ctgaataatc accataaacc acggtgcgtt tttaatattc attaaagtag
     2521 tattaatcgc caataaacag cttggaataa tgccaaataa catcacgagt gcgatagttt
     2581 caaagaattt ttgtcatttt gatttctcct acccccgaat tcgagggaat taataaacac
     2641 acactaaatc ggattaaaat cgctcttaaa gtcatcaagg cattcttgat atttccattg
     2701 accgataaca ataaaactac cgactgccac aaaaaatgct aatggataaa tcaataagta
     2761 cccgatatat ctaagagtaa gaattgacaa agaaa
//
LOCUS       DANLXF010000257         2739 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.258, whole genome shotgun sequence.
ACCESSION   DANLXF010000257 DANLXF010000000
VERSION     DANLXF010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.258"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(36..1214)
                     /gene="lpxB"
                     /locus_tag="PXD66_002318"
     CDS             complement(36..1214)
                     /gene="lpxB"
                     /locus_tag="PXD66_002318"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HDL6320565.1"
                     /translation="MKQQFPVIGLVAGEVSGDILGAGLIKALKVHYPNARFIGVAGSR
                     MIEAGCQSLFDMEELSVMGLAEVVKHLPRLLKRRKQVIDEMLKLKPDVFIGIDAPDFN
                     LTVEEKLKASGIKTIHYVSPSVWAWRQKRVFKIARAANLVLAFLPFEKAFYDKFNVPC
                     RFIGHTMADAIPLEPNRTEACLALNIDESKRYMAILVGSRGSEIQFLAEPFLKAAQLL
                     KARFPDLQFLVPMVSQKRMEQFNAIKQQVAPELELNIIQGKARQAMIASECTLLASGT
                     AALEAMLCKSPMVVGYRMKPLTYWLAKKLVKTDYISLPNLLAQAPLVPELIQEACNPE
                     NLAQELSLFLADDEKNQQHKALLKQQFMQLHQSIQCNADEQAAQAVIDLLNGSTSGQI
                     "
     gene            1348..2442
                     /locus_tag="PXD66_002319"
     CDS             1348..2442
                     /locus_tag="PXD66_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HDL6320566.1"
                     /translation="MKKTTLAILASALIANSALAITIYDHKETGTQIEFIGSARIKWE
                     STSDKTSPVNGKATRNHVNHAVDNNGSRFGFRLTQQVGHGFYALGRVEWRMRGDSSSQ
                     HNFDHIYDHQLYAGIGHKQYGELTYGNMTNIADEVKQTDLPNTLSLSDGLLTGSSRRT
                     LQYVYKGIEGLKLGAFYGGNSQRGNNGLDLVNKRKDIWGAAAIYNYKIDDNQSLKLAT
                     GATRERSEQSIGNYERIALAFGTAYTYGKTTLGLDLERRETEDQNAIGHKRIEKEVRT
                     VLLHRLTDQWNAYTMYAYKENKLNAVGLANDSKVKRNQFMLGTEYYIVPKHLKGFVEW
                     QATRAKHYTNDVKTAKSRNYTTVVGLRAYW"
BASE COUNT          901 a          572 c          489 g          777 t
ORIGIN      
        1 tttccacata aattttcctt tttacaaaaa actaactaaa tttgaccgct tgtcgagccg
       61 tttaggagat cgataaccgc ttgggcagct tgctcatcag cattacattg aattgattga
      121 tgtagctgca taaactgctg ttttagcaat gctttatgtt gttggttttt ctcatcatca
      181 gctaaaaata gagataactc ttgagctaaa ttttccgggt tgcatgcttc ttggatcaat
      241 tctggtacca atggggcttg agccaataaa ttcggtaagg agatataatc ggttttcact
      301 aatttttttg ctaaccaata agtcagcggt ttcattctat aaccaactac catcggagat
      361 ttgcacaaca ttgcctctag tgctgctgtt cccgatgcca aaagagtaca ttctgacgca
      421 atcatagcct gacgagcctt gccttgaata atattcagtt ccaactcggg agctacttgc
      481 tgtttaattg cattaaattg ctccattcgc ttttggctga ccattggcac taaaaactgt
      541 aaatccggaa aacgggcttt taacaactga gcagccttca aaaaaggctc agccaaaaac
      601 tgaatttcac tgccacggct tcccaccaaa attgccatat atcgcttgga ttcatcaata
      661 tttaacgcta aacatgcctc tgtacgattt ggttcgagtg ggatcgcatc tgccatcgta
      721 tgcccgataa aacgacaagg cacattgaat ttatcataga acgctttctc aaatggcaga
      781 aatgccaaca ctaaattagc tgctctggca attttaaaca cccgcttttg tcgccaagcc
      841 cacaccgaag ggctaacata atggattgtt ttaatcccac tcgctttgag tttttcttca
      901 acggttaaat taaaatccgg tgcatcaata ccgataaata catctggttt caatttaagc
      961 atctcatcaa taacctgttt acgacgctta agtaaacgag gtaaatgttt aaccacctct
     1021 gctagcccca ttacagaaag ctcttccata tcaaacaaag actggcagcc agcctcaatc
     1081 attcgagagc ctgcaacacc gataaatcga gcattcgggt aatgtacttt taacgcctta
     1141 attaacccgg caccaagaat atcgccagag acctcaccag caactaagcc aataacgggg
     1201 aattgttgtt tcatattaaa tattattgaa gatctgtaag tgactctatt ataaccaatc
     1261 atcatataaa ctaacaaaac attattgtct agcaatgaat attcacatac actacctccc
     1321 tttatctatt catatggagt gatttttatg aaaaaaacaa cattagcgat tttagcatca
     1381 gccttaatcg caaactcagc tctagctatt acgatttacg atcataaaga aaccggtaca
     1441 caaattgaat ttattggttc tgctcgtatt aagtgggaaa gcacctcaga taaaacctca
     1501 ccggtaaatg gtaaagctac ccgcaaccac gttaatcacg cagtggataa caacggctct
     1561 cgttttggtt tccgtttaac ccagcaagtg ggtcacggat tctatgcttt aggtcgtgtg
     1621 gaatggcgta tgcgtggcga ttcaagctca caacataact tcgatcatat ttatgatcat
     1681 caactctatg caggaatcgg gcataaacaa tacggtgaat taacatacgg caatatgact
     1741 aatattgccg atgaagtaaa acaaactgac ttaccaaata cactcagctt aagtgatggc
     1801 ttacttaccg gttcatcacg acgtacgctt caatatgttt acaaaggcat cgaaggatta
     1861 aaactcggag cattctatgg cggaaatagc caacgtggaa acaatggctt agatttagtc
     1921 aataaacgta aagatatttg gggtgcagca gcaatttata actataaaat tgatgataac
     1981 caaagcttaa aattagcaac aggtgcaacc cgtgagcgtt ctgagcaatc tatcggcaat
     2041 tatgaacgca ttgctcttgc cttcggtaca gcttatacct acggaaaaac tacccttggc
     2101 ttagatttag aacgccgtga aacggaagat caaaatgcta tcggtcacaa acgtatcgaa
     2161 aaagaagttc gcactgtgtt actccatcgt ttaaccgatc aatggaatgc ttacacaatg
     2221 tatgcttata aagaaaacaa acttaatgct gtaggcttgg caaatgatag caaagttaaa
     2281 agaaaccaat ttatgttggg aacggaatat tacattgttc caaaacactt aaaaggcttt
     2341 gttgaatggc aagcaactcg tgctaaacac tatactaatg atgtaaaaac cgcaaaatct
     2401 cgcaattata cgactgttgt aggcttacga gcatattggt aataaaactg aaaagcgtac
     2461 tagaatgatc tgcacctaaa agttgggcta accaactgat taaggtgcag attttttatg
     2521 attaaataca actaatcctt caaacaagaa gctattgcat ttatcttcaa agtggtaaaa
     2581 accgcttact cacctattag catcttcagc tttaagaaac aacactaaaa ttttagtgtg
     2641 aactaatata atcataatgg caataactga gcagccaaaa cgcctaaatt taaattatct
     2701 gttgcaaaag aggttaaact gcaaaaagtg atgaggggt
//
LOCUS       DANLXF010000258         2729 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.259, whole genome shotgun sequence.
ACCESSION   DANLXF010000258 DANLXF010000000
VERSION     DANLXF010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.259"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(34..2661)
                     /gene="alaS"
                     /locus_tag="PXD66_002320"
     CDS             complement(34..2661)
                     /gene="alaS"
                     /locus_tag="PXD66_002320"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDL6320567.1"
                     /translation="MKTTSDIRQSFLEFFQTKGHTIVPSSSLVPENDPTLLFTNAGMN
                     QFKDVFLGLEKRPYSRATTAQRCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYF
                     KQDAIKFGWEFLTSPQWLGLPKEKLYVTVYETDDEAYDIWHKEVGVPSEHIIRIGDNK
                     GAPYASDNFWAMGDTGPCGPCTEIFYDHGPEHWGGLPGSPEEDGDRYIEVWNIVFMQF
                     NRLADGTMEKLPKPSVDTGMGLERMTAVMQHVNSNYETDIFQTLIKEVATLLNVKDLD
                     NKSLRVVADHIRACSYLIADGVLPSNEGRGYVLRRIIRRAVRHGNILGAKEAFFYKLV
                     PTLATVMGQAGEVLTQKQAHIQKTLKAEEEQFARTLERGLNLLEEALAKVENNTLSGE
                     VAFKLYDTYGFPLDLTADVCRERDIKIDEDGFNAEMTAQRERAKASSSFGTDYNNVIK
                     VDSKTCFSGYTETSLNDATVIGLFSNGKSVESIQSGENAVVILDKTPFYAEMGGQVGD
                     SGQISAEICNFEVVDTQKYGQVFGHIGQLTSGSLSVGDKVNTIVDRERRHAITLNHSA
                     THLLHSALRQVLGDHVAQKGSLVSENILRFDISQPEAITKSQLEEVERIVNAKVRENI
                     QVIIEEMDIESAKAKGAMALFGEKYGEVVRVVEMSDFSIELCGGTHVKQTGDIGLFKI
                     TSEGAVAAGVRRIEAVTGENAIAWLHTLQQAVQQSAELLKADSHSFVEKIVQLQEKAK
                     RTEKELQQLKDKLAAQAGSDLAKQATQINGVNVVIQQLENVEPKALRTMVDDLKNQLG
                     SAVVVFATTAEDKVNLIVGVTKDLTDKVNAGQLVGAMAEKVGGKGGGRPDMAMAGGSE
                     PQHLLQALSLAQEWITAKL"
BASE COUNT          749 a          628 c          476 g          876 t
ORIGIN      
        1 taaaggcgag ttcagctcgc cttgttatta tttttaaagt tttgctgtaa tccattcttg
       61 agcaagtgac aatgcttgca ataaatgttg tggttctgag cctccggcca ttgccatatc
      121 aggacgtccg ccccctttac ctcccacttt ttcagccatt gcaccaacta attgaccagc
      181 attgacttta tcggttaaat ctttggtcac acctacaatc aaattgactt tatcttccgc
      241 cgtagtcgca aatacaacaa ctgcactgcc taattgattt tttaggtcat caaccatcgt
      301 tctcaatgct tttggttcaa cattctctaa ttgttgaatc actacattca cgccattgat
      361 ttgggtagct tgtttggcta aatctgaacc tgcttgtgct gcaagtttat cttttagctg
      421 ttgaagctct ttttctgtac gtttagcctt ctcttgtaat tgaacaattt tctcaacgaa
      481 tgaatgactg tctgctttta acaattcagc actttgttga acggcttgtt gtaaggtatg
      541 caaccaagca atcgcatttt caccagtgac agcctctata cggcgtacac ctgctgctac
      601 cgcaccttca gaggtaattt tgaataagcc aatatcaccg gtttgtttaa cgtgtgtgcc
      661 gccacaaagt tcaatcgaga aatctgacat ttccactaca cgcactactt cgccatattt
      721 ttcaccaaat aacgccattg cacctttcgc ttttgcagat tcaatatcca tttcttcaat
      781 gattacctga atattttcac gcactttagc attcacgata cgttcaactt cttcaagttg
      841 tgatttagta attgcttccg gctgagaaat atcaaaacgt aaaatatttt ctgacactaa
      901 tgagcctttt tgagcaacgt ggtcgcctag cacttggcgt aacgcagaat gcagtaagtg
      961 ggtagcactg tggtttaatg taatagcatg acgacgctct ctatctacga tcgtattaac
     1021 tttgtcgcca acagaaagtg agccggaagt taattgccca atatgcccga atacttgacc
     1081 atatttttgc gtatctacta cctcaaaatt gcaaatttct gcggaaattt gaccgctatc
     1141 tcccacttgt ccgcccattt ctgcatagaa tggggtttta tctaaaatca ctaccgcatt
     1201 ttcacccgat tgaatagact caaccgattt tccattacta aataacccaa ttacagtagc
     1261 atcatttaag ctggtttcag tatatccact aaagcatgtt ttactgtcca ctttaatcac
     1321 attgttataa tctgtgccaa agctactgct ggctttcgca cgctcacgtt gtgcggtcat
     1381 ttctgcattg aagccgtctt cgtcaatttt aatatcacgt tcacggcaaa catctgcggt
     1441 taaatctaac gggaagccat aagtatcata aagtttgaac gcaacttcgc cagataaggt
     1501 gttattttca accttcgcta atgcttcttc taataaattc aatccacgct caagagtacg
     1561 ggcaaattgc tcttcttctg ctttgagtgt cttttgaata tgcgcttgtt tttgggtcag
     1621 cacctcacca gcttgcccca ttaccgttgc aagtgttggc accaatttat agaaaaaggc
     1681 ttcttttgca cccaaaatat taccgtgacg aaccgcccgg cggataatac ggcgtaatac
     1741 atagcctctt ccttcatttg aaggtaaaac accatcggca atcagatagg aacaagcacg
     1801 aatatggtct gccactacac gtaaagattt attatctaaa tctttcacat taagcaaagt
     1861 agcgacttct ttaattaaag tttggaaaat atccgtttcg tagttggagt ttacgtgttg
     1921 cattaccgca gtcatacgtt ctaaccccat ccctgtatct acagaaggct taggcagttt
     1981 ttccatcgta ccgtctgcta aacgattgaa ctgcataaat acaatattcc atacctcaat
     2041 atagcggtcg ccatcttctt ctgggctacc cggtaagcca ccccaatgtt caggcccgtg
     2101 atcatagaaa atctcggtac aagggccaca agggccggta tcgcccatcg cccagaagtt
     2161 atctgaagca taaggtgcgc ctttattatc gccaatgcga ataatatgtt ccgatggtac
     2221 acctacttct ttatgccaaa tatcgtaggc ttcatcatca gtttcataca cagttacata
     2281 taatttttct tttggtaagc caagccattg tggagaagtt aaaaactccc aaccgaattt
     2341 gattgcatct tgcttgaagt aatcaccaaa gctgaaattc cccatcattt caaaaaaagt
     2401 atgatggcga gcggtataac ctacgttttc taaatcattg tgtttaccac ctgcacgtac
     2461 acaacgttgt gccgtagtcg ctctgctata agggcgtttt tctaacccaa gaaaaacatc
     2521 tttgaattgg ttcatgcccg cattggtaaa taataatgtc ggatcattct cgggaacaag
     2581 agagctgctt ggaacaatcg tatgtccttt agtttggaaa aactctagaa aagattgtct
     2641 gatatctgaa gttgttttca ttaaaaatca ctctttttgt aaatagaagt taaacattta
     2701 gttatcttgc tattttacac attttttat
//
LOCUS       DANLXF010000259         2680 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.260, whole genome shotgun sequence.
ACCESSION   DANLXF010000259 DANLXF010000000
VERSION     DANLXF010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.260"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(164..1402)
                     /gene="pepT"
                     /locus_tag="PXD66_002321"
     CDS             complement(164..1402)
                     /gene="pepT"
                     /locus_tag="PXD66_002321"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDL6320568.1"
                     /translation="MKQSLLNRLLRYTQIHTTSDPKSQTVPSSQQQWDLLHLLKTEVE
                     AMGLSEIYLDPNGYLFASLPANIDKTLPVLGLIAHVDTSPDFNGENVKPQVIEAYQGG
                     NILLNGSGDYLSPTDFPNLNKLIGDTLVTTDGTSLLGADDKSGIAIILSAIEYLQANP
                     HIPHGKIRIGFTTDEEIGRGADHFDVNAFGADVAFTFDGGPLGELQYENFNADAATVT
                     FFGRSVHPGTAKGKMINALERACEFQHRLPPHLTPATSEGREGFIMLHGIEGGIDKAE
                     IHYILRSFERDELAAFGKMLNDTLAEMQQIYGEHCGKVEIVEQYRNMREVLDDYGYLV
                     EIAENAMREQGIEPNLEPIRGGTDGSRLSFMGLPCPNLFTGGENFHGRFEFISVDTME
                     KATKVVIDICRKFADYDFNK"
     gene            complement(1422..1811)
                     /locus_tag="PXD66_002322"
     CDS             complement(1422..1811)
                     /locus_tag="PXD66_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HDL6320569.1"
                     /translation="MYMLDTNTVSYFFRGNANVVAKLKSINPERLCISSVTAAELVYG
                     VAKRNNGQLSQFLDYFLSSVKVLDWNYRCAELYGKLRAEMEKNGKVMGVQDQMIASHA
                     LAEECILVSSDQAFKMVPDLKLENWLH"
     gene            complement(1812..2072)
                     /locus_tag="PXD66_002323"
     CDS             complement(1812..2072)
                     /locus_tag="PXD66_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HDL6320570.1"
                     /translation="MEKIARLFRNGRNQAVRLPVEFEFDAEQVYVSKKENGDILLSLR
                     SRNTEHWQRLFELLPAVVCDETFLDTKERNQSFEQRDPFEVA"
     gene            complement(2188..2400)
                     /locus_tag="PXD66_002324"
     CDS             complement(2188..2400)
                     /locus_tag="PXD66_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HDL6320571.1"
                     /translation="MRDEYDFSNAVKNPYAKGLKSKSVVTIRIEDDVISYFKQLAEEV
                     NLPYQTLINLYLKDCVEQKRKPTISW"
     gene            complement(2401..>2680)
                     /locus_tag="PXD66_002325"
     CDS             complement(2401..>2680)
                     /locus_tag="PXD66_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HDL6320572.1"
                     /translation="INGFEWDSEKALRNQQKHGVSFIEAVSVFYDNNAMLIPDPDHSS
                     REERFLLLGRSNKDNLLVVVHCERNTNIRIISARLATKRESGQYKGVH"
BASE COUNT          770 a          627 c          488 g          795 t
ORIGIN      
        1 ttaaaaacgc tacgaacctc gtagagtctc aacaggtgca tattctatgg atttcagatt
       61 taatgtcaaa taaatttgta ggaaatctgt aaaaaatcgt ttaattggtg aaaaataacg
      121 ctttttgcaa atttttcatc aaatttgacc gcttgcagcc gccttatttg ttgaaatcat
      181 aatcagcaaa tttccgacaa atatcaatta caacttttgt ggctttttcc atcgtatcaa
      241 ccgaaataaa ctcaaatcgt ccgtggaaat tttcgccgcc ggtaaataaa ttcgggcaag
      301 gcaagcccat aaatgaaagg cgtgagccat cagtaccgcc acggatcggt tctaaattcg
      361 gctcaatgcc ttgttcacgc attgcgtttt cggcaatttc aaccaagtag ccgtaatcgt
      421 ccagcacttc acgcatattg cgatattgct cgacaatctc gacttttccg caatgttcgc
      481 cgtaaatctg ttgcatctct gccagcgtat cattcagcat tttgccgaat gcagctaatt
      541 catcacgctc aaagctacgt aaaatatagt gaatttccgc tttatcaatt ccaccctcaa
      601 tgccgtgcaa cataataaag ccttcacgcc cttcgctggt ggcaggggtt aaatggggag
      661 gcagacggtg ttggaactcg caagctcgct ctaaggcgtt gatcatcttg ccctttgccg
      721 tgccggggtg aacgctacgc ccgaagaacg taacggtcgc tgcatcggca ttgaaatttt
      781 catattgcaa ttcgcccaga ggcccgccat caaaggtaaa ggcgacatcg gcaccgaatg
      841 cgtttacatc aaaatgatct gcgcctcgac cgatctcttc atcggtggta aagccgatgc
      901 ggatcttgcc gtgcggaata tgcggattgg cttgtaaata ctcgatagcc gagagaataa
      961 tcgcaatgcc ggatttgtcg tctgcgccaa gcaaagaggt gccgtcagtg gtgactaagg
     1021 tgtcgccaat cagcttgttt aaattcggga aatccgttgg cgaaagataa tcgccactgc
     1081 cgtttagcaa aatattgccg ccttgataag cctcaatcac ctgtggtttc acgttttcac
     1141 cattaaaatc gggcgaagta tcaacgtggg caatcagccc aagcaccggc agggttttgt
     1201 caatattggc aggcaagctg gcgaacagat agccgttggg gtcaagataa atctcgctta
     1261 gccccatcgc ctctacttcc gttttcagca gatgaagcaa atcccactgc tgttgcgagc
     1321 ttggcacagt ttggcttttc ggatctgaag ttgtgtgaat ttgggtgtaa cgtagtaaac
     1381 ggttaagaag ggattgtttc attgtttttc cttactatag tttagtgtaa ccaattctcc
     1441 aatttcagat caggtaccat tttaaatgcc tgatcactag aaactaaaat acactcttca
     1501 gcaagagcgt gactagcaat catttgatcc tgcacgccca ttactttgcc atttttctcc
     1561 atctctgccc gtaactttcc ataaagttca gcacaacgat aattccaatc taaaaccttt
     1621 acactcgata aaaaataatc taaaaattga gaaagctgcc cattatttct tttcgccaca
     1681 ccataaacca attcagcagc agttacagat gaaatacaaa gcctctcagg attaatggac
     1741 ttcaatttcg ccaccacatt tgcatttcca cgaaaaaaat agcttacagt attcgtatct
     1801 aacatataca tttaagctac ctcaaaagga tcccgttgct caaaggattg gtttctctct
     1861 tttgtatcta aaaatgtttc atcacataca acagcaggca atagctcaaa caatcgttgc
     1921 caatgctcgg tatttctcga tctcaatgat aataaaatat cgccattttc ttttttagaa
     1981 acatagactt gctctgcatc aaactcaaat tcaaccggta aacgcactgc ttggttccgc
     2041 ccgttacgga ataatctcgc aattttttcc atcttacttt accctccgat agatagtgta
     2101 tatgccaaag tatatgccta ttttatacaa ctggcaagat aacccattct caaatactca
     2161 ataacatact gatttcaaat cataatatta ccaactaata gtcggtttac gtttctgctc
     2221 aacgcaatct ttcaaataaa gattgatcaa cgtttggtaa ggcaaattca cctcttcggc
     2281 aagttgttta aaatagctga taacatcgtc ttcaatccga atagtaacca cacttttgct
     2341 ttttagccct tttgcgtaag ggtttttcac tgcattagaa aaatcgtatt catctctcat
     2401 ttaatgtact cctttatatt gcccactttc gcgttttgtt gccaaacgtg ctgaaataat
     2461 ccgaatgttg gtatttctct cgcaatgcac aaccacaagt agattatctt tattgctacg
     2521 cccgagtagc aaaaaacgct cttccctaga tgagtgatct ggatccggga ttaacatcgc
     2581 attattatca taaaatacgc ttaccgcctc gataaaacta actccgtgtt tttgttgatt
     2641 acgaagagct ttctcactat cccattcaaa accattaatt
//
LOCUS       DANLXF010000260         2676 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.261, whole genome shotgun sequence.
ACCESSION   DANLXF010000260 DANLXF010000000
VERSION     DANLXF010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.261"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            139..579
                     /locus_tag="PXD66_002326"
     CDS             139..579
                     /locus_tag="PXD66_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDL6320573.1"
                     /translation="MTACKNGLGFLVLRLFLAYEFFEAGLSKLNGENWFASIQESFPF
                     PFNVLPADLSWAIASGAEILLPILLTLGLFTRFSALGLMILTAVAWYAVHAGNGYNVC
                     NNGYKMALIYLVMLVPLLLQGAGSLSLDFLLQKKSPTKRWLKFL"
     gene            605..892
                     /locus_tag="PXD66_002327"
     CDS             605..892
                     /locus_tag="PXD66_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320574.1"
                     /translation="MKKATLMALAGALTMATATVNAETKSVKADACMEVKDGKCVKTK
                     ATEGKCGEGKCGATSSEPKATEGKCGEGKCGATAPKAAEGKCGEGKCGASK"
     gene            993..1916
                     /locus_tag="PXD66_002328"
     CDS             993..1916
                     /locus_tag="PXD66_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HDL6320575.1"
                     /translation="MLKFTGAGLGYRRDLADGFLSLTDNNIINFMEVAPENWLKMGGQ
                     ARYNFDKVAEKIPVIIHGLSLSLGGQAPLDMTLLKGIKNMQQQYNSFFFSDHLSYCEC
                     EGHLYDLLPMPFTDEAVHHVANRIKQVQDYLGMQISLENTSYYLHSPTSTMNEVEFLN
                     AIAQEADCGIHLDVNNIYVNGVNHGLLDPFVFIDQVDKSRVNYIHIAGHDEDHGAAED
                     VKMTEGESFNMVKGELRHLPTLLVDTHGEAVQKTVWDLLEYTYEHLPHIPPTLLERDF
                     NFPPFQELISEVQHIMSLQKKYAKTRLKNAA"
     gene            1906..2646
                     /locus_tag="PXD66_002329"
     CDS             1906..2646
                     /locus_tag="PXD66_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="HDL6320576.1"
                     /translation="MPLKLVEEHKPLLKEIQAEFANAIRHNDVKLMSGNISPQRLGVY
                     SRLVRNNTLGFIDRCYVQLPQHLSPEEWVAVKEKFIREGKAHSPFFQDIAGEFLNFCR
                     ENGCMPPHLLELMDFENAQLLAEVSMATVPSEFEWDNDTIMQFSGAAELRQYAVNFIR
                     SEFKQFDFEPTNVLIWRDTEFGIYYNRLEELDFFLLSSLQEGANSLNGLLAELSEFTE
                     QNLELKALLRQLWEKWVEAEVIYPVYPE"
BASE COUNT          800 a          531 c          571 g          774 t
ORIGIN      
        1 ctataaaaat aggttataat ttttcacaca ttttttgtaa taaacgtata cttattctgt
       61 ctaataatca catttttagt taggagcatt tttatgtcat cattttcgtc atcattttca
      121 actcagtcag cttgctcaat gacagcttgt aaaaacggct tgggctttct tgtattacgt
      181 ctatttcttg cttatgaatt ttttgaagca gggttatcaa aactaaacgg tgaaaattgg
      241 tttgcctcaa ttcaagagag cttcccattc ccctttaacg tactaccagc cgatcttagc
      301 tgggctattg ctagcggagc ggaaatctta ctgcctattt tactcacttt gggtctgttc
      361 acccgcttca gtgcgttagg attaatgatc ttaacagcgg tagcgtggta tgctgttcac
      421 gctggcaatg gctacaatgt ctgcaacaat ggctataaaa tggcactcat ttatttagtg
      481 atgttagtac cacttttatt acaaggtgct ggctcgctct ctctcgattt tctgttacag
      541 aaaaaatccc caaccaaacg ctggttgaaa ttcctttaat cccttaattt atggagaatt
      601 tttaatgaaa aaagcaactt taatggcatt agctggtgca ttaactatgg caaccgcgac
      661 tgtaaacgct gaaactaaat cagtgaaagc agatgcttgt atggaagtaa aagatggcaa
      721 atgtgtaaaa accaaagcta cagaaggtaa atgtggcgaa ggcaaatgtg gagcaacctc
      781 ttctgagcca aaagcaaccg aaggtaaatg cggcgaaggg aaatgtggag caacggcacc
      841 aaaagcggca gaaggtaaat gcggtgaagg caagtgtgga gcttctaagt aagtagcgaa
      901 ggtgcgtttt ctaaacgcac ctaacctctt ataaacacaa tctacaagcg gttaaattcc
      961 gctgattttt tgcaaattaa aggaacaaga cgatgttaaa atttactggt gccggtctag
     1021 gctatcgccg tgacctagcg gacggatttt tatctctcac agataacaat ataattaatt
     1081 ttatggaagt agccccagaa aactggctga aaatgggcgg tcaggctcgg tataacttcg
     1141 ataaagtcgc tgaaaaaatc cctgttatta ttcacggttt atccctctct ctcggcgggc
     1201 aagcgccttt agatatgacg ctactaaaag ggatcaagaa tatgcagcag cagtataact
     1261 cctttttctt ctctgatcat ttaagctatt gcgaatgtga agggcattta tatgacctgc
     1321 tgccaatgcc gtttaccgat gaagccgtac atcacgtggc aaatcgcatt aaacaagtgc
     1381 aagattattt aggtatgcaa atttcccttg aaaatacgtc ctactatctg cattcaccaa
     1441 ccagtacgat gaatgaggta gagtttctca atgcaattgc ccaagaagcg gattgtggca
     1501 ttcaccttga tgtgaataat atttatgtta atggtgtcaa tcacggcttg cttgatccat
     1561 ttgtatttat tgatcaagtg gataaatctc gtgtcaatta tattcatatt gcaggccacg
     1621 atgaagatca cggtgcggcg gaagacgtta aaatgaccga gggtgaatca tttaatatgg
     1681 taaaaggcga actacgtcat ctgccaactt tactggtcga tacacacgga gaagctgtac
     1741 aaaaaactgt gtgggattta ctagagtaca cctatgaaca cctaccgcat attccaccaa
     1801 cactgttaga acgggatttc aacttcccac cgtttcaaga actgattagt gaagtacagc
     1861 acattatgag cttacagaaa aaatatgcaa aaacgaggct aaaaaatgcc gcttaaactc
     1921 gttgaagaac acaaaccgtt actcaaagag attcaagccg agtttgcgaa tgcaattcgc
     1981 cataacgatg taaagctgat gagtggtaat atctctccac agcggcttgg cgtctattct
     2041 cgcttggttc gcaataatac tctcggtttt atcgaccgtt gttatgtaca gctccctcaa
     2101 catctaagtc ctgaagaatg ggttgccgtt aaagaaaaat ttatacgaga aggtaaggct
     2161 cattcgccct ttttccaaga tattgctggc gaatttctca atttttgccg agaaaatggc
     2221 tgtatgccac cacacttgct tgagttgatg gactttgaaa atgctcaatt actggctgag
     2281 gtatcaatgg caaccgtacc atcagaattt gaatgggata acgatactat aatgcagttt
     2341 tctggtgcag ctgagctgcg tcaatatgcg gtaaacttta tccgtagcga gttcaaacag
     2401 tttgactttg agccgaccaa cgtactgatt tggcgagata cggaatttgg catctattac
     2461 aaccgcttgg aagagctgga tttcttcctg cttagctcgt tgcaagaggg ggcaaattcc
     2521 ctgaatggct tactagcaga actcagcgag tttaccgagc aaaatctgga gctaaaagcc
     2581 ttgttacgcc aactttggga aaaatgggtt gaagcagagg tgatttatcc tgtctatcct
     2641 gaatgaggat aaggtatgtg atatgtactg acatac
//
LOCUS       DANLXF010000261         2632 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.262, whole genome shotgun sequence.
ACCESSION   DANLXF010000261 DANLXF010000000
VERSION     DANLXF010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.262"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(61..237)
                     /locus_tag="PXD66_002330"
     CDS             complement(61..237)
                     /locus_tag="PXD66_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320577.1"
                     /translation="MTEEDKEYLQTKIENEGFEYAFVSYSDFEEVQDEKFHGLRKAYL
                     KARSELAEYIDIED"
     gene            complement(491..1192)
                     /locus_tag="PXD66_002331"
     CDS             complement(491..1192)
                     /locus_tag="PXD66_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320578.1"
                     /translation="MSTQISTQTISFNNQALITFEQNGTHYTAMKPICENIGLTWHAQ
                     YERIKRDEILSQGILIIRIPTNGGDQQMICLPIEYLNGWLFGIDINRCKPEIRETLIK
                     YKKECYQALHDYWFKGKAERSQIAPIVEPKIPMELTKKEWLRFASMWYALYNSLEVLA
                     TLEKPLRAIGSPFGATAYTHATEYQTTLGVMKRILEPMLADFEVDPFEEAHYHKALTT
                     LRQYQPKGLGGLVRI"
     gene            1737..1952
                     /locus_tag="PXD66_002332"
     CDS             1737..1952
                     /locus_tag="PXD66_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320579.1"
                     /translation="MQTEKRGRGRPKSGLTLQELQAKSDAKRGVRLKSFKFHEDFIAQ
                     LEQLAEQHGISQTQVIVQAVEQFSKGA"
     gene            complement(2004..2222)
                     /locus_tag="PXD66_002333"
                     /pseudo
     CDS             complement(2004..2222)
                     /locus_tag="PXD66_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014390712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
     gene            2414..2602
                     /locus_tag="PXD66_002334"
     CDS             2414..2602
                     /locus_tag="PXD66_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320580.1"
                     /translation="MKELFKQWLINQDSPFINSCGVECILSKVDDRLNIINANEEETE
                     TLIEWRNAFLQDVSVFIA"
BASE COUNT          723 a          497 c          587 g          825 t
ORIGIN      
        1 gttagctaca agcggttggt tgtcaagtgt tcatttgttc aatgaacagt tcatcagtgg
       61 ttaatcttca atatcaatat attccgctaa ctcacttcga gcttttaaat aagccttgcg
      121 aagcccgtgg aatttttcat cttggacttc ttcaaagtca ctataactga cgaaagcgta
      181 ttcaaaacct tcgttttcaa ttttagtttg aagatattct ttgtcttctt ctgtcatttc
      241 gtttctccta ttgagagtgg ataaaaataa acactgcacg ggcagggtcg gggaagcatt
      301 atcgctgtgc gatagtgcat ggtttgccat cgcagtcgtt gtgtaactct aagtagtcgg
      361 cagcaactgc aaaggcggtt aaaaagagta tgatttttgc gtatttcatc gcctaatcct
      421 tgaattttgg gtgcaggaaa ccgccgcttg atgtttcaca agcggtcgga ttgtgtgatt
      481 tttttgcaaa ttaaattcgg actaagccgc ctaagccttt cggctggtat tgccttaagg
      541 tagttagggc tttgtggtag tgagcttctt caaacggatc gacctcgaag tcggctaaca
      601 tcggttcaag tatccgtttc attacgccaa gcgtggtttg atactcggtg gcgtgagtgt
      661 aggcggttgc cccaaacggc gaaccgatag cacgcaacgg tttttctaac gtagcaagta
      721 cttcaaggct gttgtagagg gcgtaccaca ttgaagcgaa acgtagccat tcttttttgg
      781 ttagctccat tgggatttta ggttcgacaa tcggggcgat ttgtgaacgt tccgctttgc
      841 ccttgaacca gtaatcgtgc agggcttggt agcactcttt tttgtatttg atgagcgttt
      901 ctctgatttc gggtttgcag cgattgatgt caatgccgaa gagccagccg ttgaggtatt
      961 caattgggag gcagatcatt tgttgatcgc caccgtttgt aggtattctt atgataagaa
     1021 taccctgcga aaggatttca tctcgtttga tccgttcgta ttgagcgtgc caagtcaagc
     1081 cgatattttc gcagatcggt ttcattgcgg tgtaatgtgt gccgttttgc tcgaatgtga
     1141 ttaaggcttg gttgttgaaa gaaatggttt gagtagaaat ttgagttgac atagtcgtaa
     1201 tccttagagt ctatttgttt aaaacaagcc actttcgaca gtggcgtcgg gaggttcgaa
     1261 aacccactct aaggtaaggg ctggacgtat ttcctttcgg tcttgtatta gtcgccctcc
     1321 cgacatagtc aggattacgg atataaaaaa atcgcctagt ggcgattagt gaactatccg
     1381 ccttagagta tgaggtttcg acaccttggg cggaatagta agttaaagtt tggcggttgt
     1441 caaataaaat tattttaata aatactaaaa aatagcttgc gttttatttg gtatgtatta
     1501 aaataatctt gttttcggaa agggtctgaa aatgaagaag ccgcccttga agagagcggc
     1561 aaacatcagg aactggatta tgtatcttaa gttgttaatc ctagtgatct taatcttaat
     1621 aagcacgcca gcttattaga ttagaaagct aaaagtccta gcagagaacg ccatctctgc
     1681 taggcagttc ctaaataata taacgttccg tattaaaaat caacaaggaa ttgacaatgc
     1741 aaacagagaa acgaggtcgt ggtcgcccta aatcaggttt aacgcttcaa gaattacaag
     1801 caaaaagcga tgcaaagcgt ggtgtgcgat tgaagtcatt caagttccac gaagatttta
     1861 ttgctcaact tgaacagctt gcagaacaac acggcatttc gcaaacacag gtaatcgttc
     1921 aagcggttga gcagttttca aaaggggcgt aagcccctat aaacctaccc ttcactgttc
     1981 gatgttttta attagttgtt gcatttaatt tggtgtaatt ccaccaaatt aaaatttgga
     2041 ttattcagct cttcccaaac ttgaagaaat tcaatggtat ttttattttg aagccagttt
     2101 ttaatgagct ggtcgctgtc tccaaaagct ttgcacatat cagttaggct gatgtaatcc
     2161 tcaccatcac gagcggttac tttgacctcc acgccttgta caatcattac gctatttgcc
     2221 atttcgtttc tccgttttta ggtacaaaaa aagccgttgg tggacggctt gaaagtgcgg
     2281 ttatcttaat ccgaagtttg gcggtgtcaa gcctcaatat ctatttttgc gatctgcatc
     2341 gaaaaaataa atttttgttc tgacagtaaa acgctttcgt agtaatctca taaaaaaata
     2401 taaggagaac actatgaaag agcttttcaa acaatggcta atcaatcagg actcgccatt
     2461 cattaatagc tgtggcgttg agtgtatttt gtcaaaagtt gatgaccggc taaacataat
     2521 caacgccaac gaagaagaaa ccgaaactct tatagagtgg cggaatgctt ttctgcaaga
     2581 tgtttcggtt tttatagctt aggtactata aatcgtcttc tttaacccaa at
//
LOCUS       DANLXF010000262         2627 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.263, whole genome shotgun sequence.
ACCESSION   DANLXF010000262 DANLXF010000000
VERSION     DANLXF010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.263"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            116..2464
                     /locus_tag="PXD66_002335"
     CDS             116..2464
                     /locus_tag="PXD66_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDL6320581.1"
                     /translation="MLISAQFSPLVWAENNDVAVLDEVSVVGSTPSISQGSEVTLLKV
                     SDKIIAGKEFKKRSATLGNALASELGVHSNPFGGGASKPIIRGQEGARIRILQNGSDV
                     IDMSNLSPDHAVVADSLLADQVEILRGSSTLLYASSSPVGIVNVVDKRIPTAIPEKGY
                     EVELNSRFDTAAKEKVGALGATFGIGNHIAVRVEGLSRHSDNYRVPGINLGERLNYVP
                     DTYNKSKVGTLGLSFVGERGYIGASYSKRRDNYGLPGHNHKFDFCTGHIYGNKRDKYA
                     YTYLYPHLIGEENIGSNPHFHCGTNHAEDGTHSHDNPFGHAHDHTHKGPWVDLESKRI
                     DVKAELRQPFKGVDKIKASYADADYYHDEKDAGVLATRYHKQLKKDQDYGKPVNIFKN
                     RGKNTRLEVYHAPLGGLTGVWGVQYQTQKSSMNAPKDREVKFPLVENTNKQFSLFGVE
                     QYMWDSVAVELAGRMEKQKIEIEYDRNEIKRLQEHYRISGGKQVEPDLSPYDETAYAY
                     SGTLNWFFHPDYQLSFTASHNERLPTPMELYYHGQHIATNSFEYGNKDLKKEQSNNVE
                     LGLGYQAERIGYKVSVYYNHFKNYIYNENLFRENQLFMRRYNQAKARFYGVEAEASYR
                     FNDQYQATIFGDMVRGWLTNLPPLKINSDYSVFKDYLPENVEKGKDYLLYRADQNTPR
                     TPPMRLGFRFNAEFTPNWSGDLELIRTFTQRRTSQLEYITEGNTMLNVGLSYSNKWKE
                     LDYKISLNGTNLLNQPVYIHTSYHQFVPQMGRNFMLGMEMKF"
BASE COUNT          769 a          524 c          629 g          705 t
ORIGIN      
        1 tttaaaaaat ataatttttt tatggttatt attgtaaaga ctcatatttt gcagtacctt
       61 attatcctta atttttttaa ggagaaagat tgtgtttaag aaaacaggat tggcattgct
      121 gatttcggct caatttagcc cgcttgtttg ggcggaaaat aacgatgtag cggtgttaga
      181 tgaagtttcg gtcgttggca gcactccaag tatatcgcag gggagtgaag ttaccttgct
      241 aaaagtcagt gataaaatta ttgcaggtaa ggaatttaaa aagcgttcgg caacgctggg
      301 taatgcgttg gcgtcagaat taggcgtgca tagtaacccg tttggtggcg gagcgagtaa
      361 gccgattatt cgtgggcagg aaggggcgag aattcgcatt ttgcaaaacg gttcagacgt
      421 gattgatatg tcgaacctat ctcctgatca cgccgttgtt gctgattcac tgcttgccga
      481 tcaggtcgaa atcttgcgtg gttccagcac cttgctttat gccagcagtt cgccggtagg
      541 aattgtaaat gtggtggata aacgtattcc gaccgctatt cctgaaaagg gatatgaggt
      601 tgaactcaat agccgttttg ataccgccgc caaagagaaa gtcggcgcat taggggcaac
      661 ctttggcatc ggtaaccaca ttgcggtgcg agtcgaaggg ctgagtcgtc atagtgataa
      721 ctaccgtgtg ccggggatca acttaggcga gcgcttaaac tatgtgccgg acacttacaa
      781 taaatccaaa gtcggcacgt tgggcttatc ctttgtcggt gaacgaggct atattggtgc
      841 ctcatacagc aaacggcggg ataattatgg cttgccgggg cataaccata aatttgattt
      901 ctgtaccggt catatctacg gtaataagcg ggataaatac gcttatacct atctctatcc
      961 gcatttgatt ggtgaggaaa atattgggag caatccgcac ttccactgtg gaaccaacca
     1021 cgccgaggac ggcacacaca gccacgataa cccgttcgga cacgcccacg atcacactca
     1081 caaaggcccg tgggtggatt tagaatcaaa acggattgat gtgaaagccg agctacgcca
     1141 gccgtttaag ggcgtagata aaattaaggc aagctatgct gatgccgatt attaccacga
     1201 tgaaaaagat gcaggcgtgc ttgccacccg ttaccataaa cagctgaaaa aagatcagga
     1261 ttacggcaag ccggtgaata ttttcaaaaa tcggggcaaa aatacccgct tggaggtcta
     1321 tcacgcaccg ctaggtggct taaccggtgt atggggtgtt cagtatcaaa cgcaaaaaag
     1381 cagtatgaat gcgccgaaag atcgcgaagt gaaattcccg ctggttgaaa ataccaataa
     1441 acagttcagc ctgtttgggg ttgagcagta tatgtgggat agcgttgctg ttgagcttgc
     1501 cggacgaatg gaaaagcaga agattgagat tgagtatgat cgcaatgaaa tcaaacggtt
     1561 acaagaacac taccgtatca gtggcggcaa acaggttgag ccagatcttt caccctatga
     1621 tgaaacggct tatgcctatt ccggcacgct taactggttc ttccaccctg actatcagct
     1681 ttcgtttact gcctcgcaca atgaacgctt gccaacgcca atggagctgt attatcacgg
     1741 gcagcatatt gctactaatt cgtttgaata cggcaataaa gacttgaaaa aagagcagtc
     1801 aaataatgtg gagttgggct tgggctatca agctgaacgt attggctata aagtgagtgt
     1861 ttattacaat cactttaaaa actacatcta caatgaaaat cttttccgtg aaaatcagct
     1921 atttatgcgc cgttataatc aggcaaaagc ccgtttttac ggggtagaag cagaggcaag
     1981 ctaccgcttt aacgaccaat accaagccac gattttcggc gatatggtac gaggctggct
     2041 aacaaattta ccaccattga agataaatag cgattatagt gtgttcaaag attatctgcc
     2101 ggaaaatgtg gaaaaaggga aagattatct tctatatcgg gctgaccaaa acacccctcg
     2161 tacaccgcca atgcgtttag gttttcgctt taatgcggaa tttaccccaa attggtcggg
     2221 ggatttagag ctgatccgca cctttaccca acgccgtacg agccagttgg aatatattac
     2281 cgaaggtaat acgatgttga atgtcgggtt gtcgtatagc aataagtgga aagagctgga
     2341 ttacaaaatc agtctaaacg gcacaaactt actgaatcag ccggtgtata tccacacttc
     2401 gtatcaccaa tttgtaccac aaatgggacg aaattttatg ttgggaatgg agatgaaatt
     2461 ttaagttctc gacataacaa gacaagcggt tattttttct tcaaattttg caaaaaaacg
     2521 gctaaaaata accgcttgtt taaaactcag gtattggtaa tttggataaa atagtgctgt
     2581 ttttcctttc ttgttatttt cggagcttac caaaatttcc tgtttgt
//
LOCUS       DANLXF010000263         2622 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.264, whole genome shotgun sequence.
ACCESSION   DANLXF010000263 DANLXF010000000
VERSION     DANLXF010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.264"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..2616
                     /locus_tag="PXD66_002336"
     CDS             <1..2616
                     /locus_tag="PXD66_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HDL6320582.1"
                     /translation="KSFFGTKLFFKDDSTEVVLSKLAKKQTDSLLLEIEKVVASNIKV
                     KVKEGFQHFANLAENQYLRDSDIPTLNDRVRLSVLSYGDNKEHFQKYFDESLVKKIQY
                     ISSLFPVNAINAQTIRREYEQHVLNHRKHFFDVIESNPLTEQQRLSVIRNNDINMVLA
                     AAGTGKTSVMVAKALDLIDSGACKSEDILILAYNRDAATELSERLQERAREANLDLEN
                     SPSISTFHALGRRIIKECGKSVHLSKFAEDPMKLDRWFNKWLEDYIKEDPDNLAKFIQ
                     LAYQPIDPFQFKTKDEYDRYVNDNDYRTLQGERVRGYQELLIANWLFSHSVEYEYEPR
                     YVSKRRIEIGFDYKPDFSLGDGVYLEHFGIDRQGRTRADINAQEYNANIQRKRELHAE
                     HNTTLLETYHYNWVENTLYKRLEQLMNEQFIPLKPKSQQEILDALNESGIFKENKNRY
                     LKCLQAIRTERLDYQQILKRLTDAKIVYAKEYATLLMRIHDAYVKELRSANEIDFDDM
                     ILLATQLVKTGEFKPKWKHILVDEFQDISMARLELLKEIYTKGPRPIWTVVGDDWQSI
                     YRFSGGKLEVTTRFNDMVGSHTLSKLEKTYRYNNSIADTAGQFIMQNPEQYQKNVVTH
                     TKVADSCVHLYDSHVVKDEKSEANISLKASAILKLIRQKAPEATVAILARYRYLLEDA
                     KSVIKDKNVKFWTFHGSKGLEADYCILLGFFQGKVGFPNQNKEEAVVEALLPMLDSFP
                     HSEERRLFYVALTRAKKESYLIADANAPSEFINELLSPKYKIDIVSKRFQERYRTIFK
                     CNECSTGYFVMKQGKFGQFYSCTSGEVCRSSPRICEKCGRPMKLREGKFGQFWGCSGY
                     GIKGDQCTNTRKYFM"
BASE COUNT          902 a          471 c          513 g          736 t
ORIGIN      
        1 aagtctttct ttggtactaa attatttttt aaagacgata gcacagaagt tgttttatca
       61 aaacttgcga aaaaacaaac tgatagtcta ttacttgaaa tcgaaaaagt agttgcatct
      121 aatattaaag tgaaagtaaa agagggtttt caacactttg caaatttagc cgaaaatcaa
      181 tatctcagag attccgacat tcctacatta aatgatcgtg taagattgtc agtgctttcc
      241 tatggggata acaaagaaca ttttcaaaaa tactttgatg aaagtttggt taagaaaatt
      301 caatatatct cttcactttt tcctgtaaat gccataaatg ctcaaacaat tcgaagagaa
      361 tacgagcaac atgttttaaa tcatcgcaag catttctttg atgtcattga atctaatccg
      421 cttactgagc aacaaagatt gtcagtcatt cggaacaacg acatcaatat ggttcttgct
      481 gccgcgggga caggaaaaac gtctgtaatg gtcgcaaaag cattggactt aattgattct
      541 ggtgcttgta aaagcgaaga tattttgatt cttgcataca accgcgatgc agcaaccgaa
      601 ctatccgaac gcttacaaga acgtgcacga gaagccaatt tagatctgga aaatagccct
      661 agcatctcga ctttccatgc gcttggccga agaattatta aagaatgtgg aaaatcagtc
      721 cacttgtcaa aatttgccga agatccgatg aaattagata gatggttcaa caaatggcta
      781 gaggactaca tcaaagaaga tcctgataat cttgcaaaat tcattcaact tgcataccaa
      841 cctatcgatc cattccaatt caaaacgaaa gacgaatacg atagatatgt aaatgacaat
      901 gactatcgaa ccttacaagg cgaacgcgtt cgtggttatc aagagttatt gatcgcgaac
      961 tggcttttta gccacagcgt agaatatgag tatgaaccaa ggtatgtatc aaaacgccgt
     1021 atcgagattg gttttgacta caaacccgat ttctctttgg gtgatggtgt ttaccttgaa
     1081 catttcggca ttgaccgaca aggtagaact cgcgcagata ttaacgcgca ggaatataat
     1141 gccaatatcc aaagaaaacg tgagttacac gccgaacata atacaacatt actggagaca
     1201 taccactaca actgggtgga aaatacgtta tataaacgac tagagcaatt gatgaatgaa
     1261 cagttcattc ctctgaagcc aaaatcacaa caagagattt tagatgcact caatgaatca
     1321 ggaatcttca aagaaaacaa aaatcgctat ttgaagtgcc ttcaagctat tcgtacagag
     1381 cgattagact atcaacaaat cttaaaacgt ctaaccgatg cgaaaattgt ctatgctaaa
     1441 gaatatgcga cattgctcat gcgtattcat gatgcctacg ttaaagaact acgtagcgca
     1501 aatgaaatcg actttgacga tatgattttg ttggctactc aactggtgaa aacaggtgag
     1561 tttaaaccta aatggaaaca tattttagtc gatgaatttc aagatatttc tatggctcga
     1621 ttggagttat tgaaagaaat ttatactaaa gggcctcgac cgatttggac tgttgttgga
     1681 gatgactggc aatcaatcta tcgtttctct ggcggtaaac tagaagttac tacgcgattt
     1741 aatgatatgg tcggctcaca cacgctaagt aaactagaaa aaacctaccg ctataacaat
     1801 agtattgctg acacagcagg tcaattcatt atgcaaaatc ctgagcagta tcagaaaaat
     1861 gttgtaacgc atacaaaggt cgctgattct tgtgttcatt tgtatgactc tcatgtcgta
     1921 aaggatgaaa aatcagaagc caatatcagt ttaaaagcct cagcaatatt aaaactgata
     1981 cgtcaaaaag cacctgaagc cactgtcgca attttggcta gataccgtta ccttttagaa
     2041 gatgcaaaaa gtgtaatcaa agataagaat gttaaatttt ggacgttcca cggctcaaaa
     2101 gggttggaag cagattactg cattttactt ggtttcttcc aaggaaaagt cggatttccc
     2161 aaccaaaata aagaagaagc cgttgtagaa gcattgcttc ctatgctaga cagttttcca
     2221 cactcagaag aacgtaggtt attttatgtg gcattaacgc gtgcgaaaaa agaaagttat
     2281 ttgatcgcag atgcgaacgc gccatcagaa tttatcaatg aattattatc accgaaatac
     2341 aagatagata ttgtgtcaaa acgattccaa gaacgttatc ggacaatctt taaatgtaat
     2401 gaatgtagca ctggctattt cgtgatgaaa caaggtaaat ttggtcagtt ctattcttgt
     2461 acatcagggg aagtatgccg ctcttcgcca cggatttgtg aaaaatgtgg ccgtcctatg
     2521 aagctgcgag aaggtaaatt tggtcagttt tggggatgct caggatatgg aatcaaaggc
     2581 gatcaatgta cgaatactcg taagtatttt atgtaatata tt
//
LOCUS       DANLXF010000264         2603 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.265, whole genome shotgun sequence.
ACCESSION   DANLXF010000264 DANLXF010000000
VERSION     DANLXF010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.265"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(82..720)
                     /locus_tag="PXD66_002337"
     CDS             complement(82..720)
                     /locus_tag="PXD66_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDL6320583.1"
                     /translation="MRYTTEQIIEKLRQLKVVPVIALDKAEDILPLAKTLSENGLPVV
                     EITFRSEAAEEAIKLVQANYPEVLIAAGTVLTKEQVLTAKNAGADCIVTPGFNPTIVK
                     YCQVLGLPVTPGVNNPMAIEAALELGIQAVKFFPAEASGGVKMIKALLGPYGNLQIMP
                     TGGISPQNINDYLAIPNVVACGGSWFVEKSLIKEQKWEEIGKLTKEVVELVK"
     gene            complement(808..1755)
                     /locus_tag="PXD66_002338"
     CDS             complement(808..1755)
                     /locus_tag="PXD66_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDL6320584.1"
                     /translation="MKKLAILGECMIELNGEPFGEMHQTYGGDSLNTATYLARVSSPE
                     QIEVRYISALGTDKLSQGMLAAWQVDNINTDFVLRDEHRQPGLYLIQLDKQGERTFLY
                     WRNQSAARYLLQHPGYPKALSALNEVDMVYLSGISLAILPENDRTLLIAQLRELKTQG
                     VKIAFDSNYRPKLWESVAAAQAHYAELLQLVDLALVTFDDEQLLWGDVNEQATFDRLT
                     QYGIQTIVVKQGSLGATVLQNGERTFVPTVAVEKVVDTTSAGDSFNAGFLNGYLLGKD
                     VVTCCQQGNAVAGIVIQHKGAIIAKSATTHLKSQFEQSI"
     gene            complement(1772..>2603)
                     /locus_tag="PXD66_002339"
     CDS             complement(1772..>2603)
                     /locus_tag="PXD66_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005752829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL6320585.1"
                     /translation="NHNFKDKVVVITGAGGVLCAYFAKEIAKTGAKVALLDINLEAAQ
                     NFASEIADEGYIAKAYQTNVLELESIKQARDEILKDFGTCDILINGAGGNNPKATTDN
                     EFHDINLPENTKSFFELDKAGIEFVFNLNYLGTLLPTQVFAKDMLGKEGANIINISSM
                     NAFTPLTKIPAYSGAKSAISNFTQWLAVYFSKVGIRCNAIAPGFLVSNQNKALLFNAD
                     GTPTARANKILTNTPMGRFGQADELVGAILFLMDHHYSSFINGVVLPVDGGFSAYSGV
                     "
BASE COUNT          697 a          601 c          545 g          760 t
ORIGIN      
        1 aatatttgtt atgctttgta aataagagga aagagatcca ttttgggtag aaaatagctc
       61 attgcttgga actttaaatg gttatttcac caattcaacc acttccttcg tcaatttccc
      121 aatctcctcc cacttctgtt ccttaatcag ggatttctcc acaaaccaag agccgccgca
      181 ggcgactacg ttgggaatgg cgaggtagtc gttgatgttt tgtggcgaga tgccgccggt
      241 cggcataatc tgtaagttgc cgtaagggcc gagcagggct ttaatcattt tcacgccgcc
      301 actggcttcg gcagggaaaa atttaactgc ttgtatgcca agttcgaggg cggcttcaat
      361 cgccatcggg ttgttcacac ccggtgtaac aggcaaacct aacacttggc aatacttaac
      421 gatagtcgga ttaaaacccg gagtcacaat gcaatccgcc ccagcgtttt tggcagttaa
      481 gacttgctct ttggtcagca ctgtgcctgc tgcaatcaat acttccggat agttggcttg
      541 cactaattta attgcctctt cagctgcttc cgagcggaaa gtgatttcca ccaccggcaa
      601 accgttttcg gaaagggttt tcgccagcgg taaaatatct tctgctttat ccaaagcaat
      661 cacgggaaca actttcagtt gtcttaattt ttcaatgatt tgttcggtgg tgtaacgcat
      721 ttctagcctc attatgttgt attgcaagcg gtcgcttttg taaaaaattt tgcaaattta
      781 accgcttgtc gttgtgctat tgttggtcta aatggattgt tcaaactgcg atttcaaatg
      841 agtagtggcg gatttggcaa taatcgcccc tttgtgctga atgacgatac cggcaacggc
      901 atttccttgt tggcaacagg taacgacatc tttacccaat aagtaaccgt taaggaagcc
      961 ggcattaaag gaatcgccgg cggaagtggt atcgaccacc ttctctaccg caacagttgg
     1021 cacaaaagta cgctccccgt tttgcaacac cgttgccccc agagagcctt gtttcaccac
     1081 aatggtctga atgccatatt gagtgagacg gtcaaaggtg gcttgctcat tcacatcgcc
     1141 ccaaagcagt tgctcgtcat caaaggtcac gagagctaaa tcgactaatt gcaacagctc
     1201 ggcatagtga gcttgggcag cagccacact ttcccacagt ttcggccggt aattgctgtc
     1261 aaaggcgatt ttcacccctt gtgttttgag ctcacgcaac tgtgcaatca gcaaagtgcg
     1321 gtcgttttcc ggcaagatgg caaggctgat accgctcaag taaaccatat ctacctcgtt
     1381 tagtgccgag agtgctttgg gatagccggg gtgttgcaac aaataacggg cggcagattg
     1441 atttcgccaa tagaggaacg ttcgctcgcc ctgtttatcc aactgaatca gatacagccc
     1501 cggctggcgg tgctcgtcac gcaacacaaa atccgtgttg atattatcca cctgccacgc
     1561 cgctaacatt ccctgactga gcttatccgt gccgagagcg gaaatataac gcacctcaat
     1621 ctgttcaggc gaactcaccc gagccaaata ggtagcggtg ttgagcgaat ccccaccata
     1681 ggtttggtgc atctccccaa acggctcccc gtttaactca atcatacatt caccgagaat
     1741 ggcgagtttt ttcataggaa taccttctaa gttacacgcc gctataagca gagaatccgc
     1801 catcaacagg taatacaacg ccattgataa agctggaata atggtgatcc attaaaaata
     1861 ggattgcacc taccaattca tcggcttgtc cgaaacgtcc cataggggta ttcgtgagga
     1921 ttttattagc tctagctgtt ggcgtaccat cggcgttaaa tagtagagct ttattttgat
     1981 tactcactaa aaatcccggt gcaatagcat tacagcggat acctactttt gagaaataaa
     2041 ctgctaacca ttgtgtaaag ttactgattg cagattttgc tccagaatag gcagggattt
     2101 tagttaatgg agtaaaggca ttcatactgg atatattaat gatattagcg ccctctttac
     2161 ccaacatatc ttttgcgaaa acctgtgttg gtaatagtgt gcctaagtaa tttaaattaa
     2221 atacaaattc aatccctgct ttatctaatt caaaaaaaga ttttgtattt tccggtaagt
     2281 taatatcgtg aaattcatta tctgtcgttg cttttggatt attcccacct gcaccattaa
     2341 ttaaaatatc acaagtgcca aaatctttta gaatttcatc tcttgcctgt tttatgcttt
     2401 ctaattcaag tacattagtt tggtacgctt tcgcaatata gccttcatcg gcaatttcag
     2461 aagcaaaatt ctgtgctgct tctagattga tatcgagtag tgcaactttt gcacctgttt
     2521 ttgcaatttc ttttgcaaaa taagcacata gcacaccacc tgctccggta ataacaacaa
     2581 ctttatcttt aaaattatga ttt
//
LOCUS       DANLXF010000265         2602 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.266, whole genome shotgun sequence.
ACCESSION   DANLXF010000265 DANLXF010000000
VERSION     DANLXF010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.266"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..766)
                     /gene="potC"
                     /locus_tag="PXD66_002340"
     CDS             complement(<1..766)
                     /gene="potC"
                     /locus_tag="PXD66_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HDL6320586.1"
                     /translation="MKKMFRNLFMLVVFAYLYIPIIILVINSFNEDRFGLNWKGFTWN
                     WYERLFSNDTLVQATLNSLTIAFFAATFSTILGALTSIALYRYRFRGKKLVGGMLFVV
                     MMSPDIVMAVSMLVLFMILGIKLGFISLLIAHITFCLPYVVIAISSRLSDFDGKMLEA
                     AKDLGASEVTILRKIILPLALPSIISGWLLSFTISLDDVIVSSFVTSPSYEVLPLKIF
                     SLVKTGVTPEVNALATIMIIFSLTLVVLSQLVVKKKV"
     gene            complement(766..1629)
                     /gene="potB"
                     /locus_tag="PXD66_002341"
     CDS             complement(766..1629)
                     /gene="potB"
                     /locus_tag="PXD66_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005698572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HDL6320587.1"
                     /translation="MKANNKFQNGAVATIFGWLLLFVLVPNVLVLIISFLTENRQSQY
                     FVDFAFSFDAYKSLFNDTYAAVLWNSLYMAGIATFFCLIIGYPFAFFIAKMPERIRPF
                     LLFLVVLPFWTNSLIRIYGIKIFLGVKGFLNESLMALGIISEPMRLLNSEIAIIIGLV
                     YILLPFMILPLYSSIEKIDNRLLEAAKDLGANAFQRFIKVTIPLTMPGIVAGCLLVLL
                     PAMGMFYVADLLGGGKTPLVGNIIKSLFLNTNQFALGSAVSIALTILMALMLYVYYRA
                     NKLLNKKVELE"
     gene            complement(1616..>2602)
                     /gene="potA"
                     /locus_tag="PXD66_002342"
     CDS             complement(1616..>2602)
                     /gene="potA"
                     /locus_tag="PXD66_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HDL6320588.1"
                     /translation="SGCGKTTALRLIAGFEDLTEGSIILDGQDVSNVPAEQRPVNTVF
                     QSYALFPHMTIFENVAFGLRMQKVPNAEIEPRVMEALRMVRLEDRAQQKPAQLSGGQQ
                     QRIAIARAVVNKPKVLLLDESLSALDYKLRKEMQNELKALQRQLGITFIFVTHDQEEA
                     LTMSDRIIVMNAGKIAQDGTPREIYEEPSNLFVARFIGEINVFDATVIERVDATNVKA
                     NVEGRICNIKVEDLEVQPNQKLKVLLRPEDIVIEELDENESSKAIIGHIIDRNYKGMT
                     LESSVRLDHNGMNVLVSEFFNEDDPNIDHEVGQKVALTWYEGWEVVLNDEGE"
BASE COUNT          854 a          540 c          453 g          755 t
ORIGIN      
        1 agactttttt cttcactact aactggctta ataccactaa tgttaatgag aaaataatca
       61 taatagttgc tagtgcattc acttcaggtg ttacacctgt tttaaccaat gagaaaattt
      121 tcagtggtaa aacttcgtag cttgggcttg tcacgaaaga tgaaacaata acgtcatcta
      181 acgaaatcgt aaagcttaac aaccagcctg aaataatcga aggtaatgcc agtggtaaaa
      241 taattttacg caaaatagtc acttcacttg cacctaggtc ttttgcagct tctagcattt
      301 tgccgtcaaa atcgctgagg cgtgaggaaa ttgcaattac cacataaggc aaacagaaag
      361 taatgtgtgc aattaatagc gaaataaagc ctagcttgat acctaagatc ataaataata
      421 cgagcataga taccgccatt acgatatcag gcgacatcat taccacgaat aacattccgc
      481 ccactagctt tttaccacgg aaacggtaac ggtaaagcgc aatagaggtt aatgccccta
      541 aaatggttga gaatgttgcc gcaaagaacg caatagttaa tgagttcaat gttgcttgta
      601 caagcgtatc attactgaat aaacgctcat accaattcca agtaaagcct ttccaattta
      661 atccaaagcg gtcttcatta aaggagttga tcactaaaat gataattgga atatagagat
      721 atgcaaaaac aaccaacata aaaaggtttc taaacatctt tttcattatt caagctccac
      781 ctttttattt aataatttgt ttgcacgata gtaaacataa agcattaatg ccattaagat
      841 agttaatgca atacttactg ctgaacctaa tgcaaattgg ttggtattta agaacaagct
      901 cttaataatg ttacctacta acggtgtttt accgccgcct aataaatctg ccacatagaa
      961 catacccatt gccggaagta atactaataa acaacctgcg acaatgcccg gcattgttaa
     1021 tggaatagtc actttaataa aacgctggaa ggcgtttgca cctaagtctt ttgcagcttc
     1081 tagtaagcgg ttatcaattt tctcgattga tgaataaagc ggtagaatca taaaaggtaa
     1141 tagaatgtaa actaaaccaa taataatcgc aatttctgag tttaataaac gcattggctc
     1201 gctgattata cctaaagcca tcaagctttc atttaaaaag ccttttacgc ctaagaaaat
     1261 cttaatgcca taaatacgaa tcagtgagtt agtccaaaac ggtagaacta ctaaaaacag
     1321 taaaaacgga cgaattcttt ccggcatttt ggcaataaaa aatgcaaagg gatagccgat
     1381 gatcaaacag aaaaaggtgg caatgcccgc catatatagc gagttccaca atactgctgc
     1441 ataggtgtca ttaaacagtg atttataagc atcaaaacta aatgcaaagt ccacaaaata
     1501 ctgactttga cggttttctg ttaagaagct gatgattaaa acgagtacgt tcggcactaa
     1561 tacgaaaagt aataaccaac cgaaaatagt tgcgaccgca ccattttgga atttgttatt
     1621 cgccttcatc gtttaacaca acctcccagc cttcatacca agtaagagca actttttgtc
     1681 ccacttcgtg gtcaatgttc ggatcgtctt cattgaagaa ctcgctaacc aatacattca
     1741 taccgttatg atctaatctc acgctagact ctaatgtcat acctttgtag ttacggtcga
     1801 taatatgacc gataattgct ttcgagcttt cgttttcgtc taactcctca atcacaatat
     1861 cttcagggcg taacaatact tttaattttt ggttcggctg aacctctaaa tcttccactt
     1921 tgatattgca aatgcgacct tctacgtttg cttttacatt agttgcatca acacgctcaa
     1981 ttacagttgc atcaaaaacg ttaatttcgc cgatgaaacg agctacaaat aagttacttg
     2041 gctcttcgta aatctcacgt ggtgtaccgt cttgagcgat tttaccagcg ttcattacga
     2101 taatacgatc agacatggtt aaggcttctt cctgatcgtg ggtcacaaaa ataaaggtaa
     2161 tgcctaattg acgttgcaat gcttttaatt cattttgcat ctctttacgc aatttataat
     2221 ctaacgcaga aagcgattcg tctaacaaca atactttcgg tttatttacc accgcacggg
     2281 caattgcaat acgttgttgc tgaccaccgg aaagctgagc cggtttttgt tgagcgcggt
     2341 cttctaaacg caccattcgt aatgcttcca tcacacgtgg ctcaatttca gcatttggca
     2401 ctttttgcat acgcagcccg aaagccacgt tttcaaaaat cgtcatatgt gggaaaaggg
     2461 catagctttg gaatacggtg tttaccgggc gttgctccgc cggaacgtta gaaacatctt
     2521 gcccatcaag aataattgaa ccttcagtta aatcttcaaa accggcaatt aaacgtaacg
     2581 cagttgtttt accacaaccg ga
//
LOCUS       DANLXF010000266         2586 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.267, whole genome shotgun sequence.
ACCESSION   DANLXF010000266 DANLXF010000000
VERSION     DANLXF010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.267"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..410)
                     /locus_tag="PXD66_002343"
     CDS             complement(<1..410)
                     /locus_tag="PXD66_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HDL6320589.1"
                     /translation="MRSMFKKFFGKNKSQSAVKSDTIFSIEQDNEGWLYPEKASQLLN
                     TELRQKYLGLLWQQVSMNRQMFEVLYQQPIERYAEMVQLLPASESHHHSHIGGMLDHG
                     LEVIAFSAKVRQSYILPPNVAPEEQSKQRDAWTAA"
     gene            complement(514..978)
                     /locus_tag="PXD66_002344"
     CDS             complement(514..978)
                     /locus_tag="PXD66_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC family protein"
                     /protein_id="HDL6320590.1"
                     /translation="MMNTQQPKFDLYQHITDRIIAALEQGTAPWLKPWSHPECNMALP
                     RNAVSNRLYSGINTLLLWIATAEHGYKQCKWITAKKANELGGYIRKGEKATVVMNYRP
                     NEREKLDEEGNIVYDEEGNPEMEQHFFIAKHNLFNLDQCENLPAELYDPVIP"
     gene            1187..1450
                     /locus_tag="PXD66_002345"
     CDS             1187..1450
                     /locus_tag="PXD66_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme-like superfamily protein"
                     /protein_id="HDL6320591.1"
                     /translation="MRPVTRNNRGTSFDPRHNKYINTLATALLARWHIEQFSERNIPV
                     NDENLYMAHNIGLDGFHRALLGQSTRKDIENMRKNGMKPWMSI"
     gene            complement(1534..2559)
                     /locus_tag="PXD66_002346"
     CDS             complement(1534..2559)
                     /locus_tag="PXD66_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HDL6320592.1"
                     /translation="MTAGDTPHDTLKTLVGALKDTREEARKANERGDRFEQEVLRLQA
                     REAEVDSRIAGEVESRVESRLAAAWDGFNRQIEELKGRVQSGNQQQKEAEAIPVGGAS
                     SGFGTESAGQTGNVDNNGIRWVDPDDMVVTDEQGKPVTGNYSGKTKTGFPSPFKAEGE
                     SSNTGTALNNNAGTENMTGNTQIQQIKRTPYYTIPENSTLMGSTAMTALLGRVPIQGA
                     VTDPFPFKVLIGRENLIANGIDLPDVEGAIVSGTSTGDWTLSCVRSEVTSITFVFADG
                     RISNGRASGESRAVKRNQASNLTPFCLTVSLSGFNDIPHSDGNPGFLTMSAVDYVDVY
                     LMSHLAR"
BASE COUNT          711 a          585 c          498 g          792 t
ORIGIN      
        1 gcagcagtcc aagcatctcg ttgctttgat tgttcttcgg gagctacatt cgggggcaaa
       61 atatagcttt gacgaacttt agctgaaaaa gcaataactt ctaaaccgtg atcaagcatt
      121 ccaccgatat gtgaatgatg gtgtgattca gaagctggta atagttgaac catctcagcg
      181 taacgttcta ttggctgctg ataaagtacc tcaaacattt gacggttcat tgatacctgt
      241 tgccaaagta gcccaagata tttttgtcgt aattctgtat taagtaattg agaggctttt
      301 tccgggtata accacccttc attatcttgc tcaatagaaa aaatagtatc ggatttgacc
      361 gcactttgag atttattttt accaaaaaat tttttgaaca ttgaacgcat tggaagagac
      421 cgaaaatatg aatgctgtat tgaatgaaag ccgtttagcc ctttgccctt ttgggtaaga
      481 cctttcccct tggaaaggtt actcttatcc cccttagggg attacaggat cgtacagttc
      541 cgccggtaaa ttttcacact ggtctaaatt gaacagattg tgcttagcaa tgaaaaagtg
      601 ttgttccatt tccggatttc cttcctcgtc atacacaata tttccctcct catcgagttt
      661 ttctctctca ttaggacggt agttcatcac aacagtagct ttttcccctt tacggatata
      721 tccgccgagt tcattggctt ttttcgccgt aatccactta cactgtttgt agccgtgttc
      781 agcggtagca atccaaagca ataacgtatt gataccggaa tacagacggt tagaaaccgc
      841 attgcgaggt aatgccatat tgcattccgg atggctccac ggttttaacc acggagctgt
      901 accttgttcc aatgcagcaa tgattctatc agtaatatgt tggtataaat caaatttagg
      961 ttgttgagtg ttcatcatgt gtttctccaa agtttgggta aaaaaatggg gaaacacggt
     1021 taccctatgg ggaacttgtt tccccgatgg gtagtttata aaatttcaat ggccacaatt
     1081 tttctagaat catcaaagcc aacagaactg cctatggcgt agtaattacg aatagtctgt
     1141 tcatcggcat tgattagcag atacctatcg aggaagagag attgggatga gaccggtaac
     1201 gagaaataat cgtggaacca gttttgaccc tcgtcacaat aaatacatca atacattggc
     1261 aaccgcatta ttggcacgtt ggcatattga gcaattctct gagcgtaata tcccagtcaa
     1321 tgatgaaaat ctctatatgg ctcacaatat tggcttagat ggtttccatc gagcgttatt
     1381 agggcaatct actcgtaaag acattgagaa tatgcgtaag aatgggatga aaccttggat
     1441 gtccatttag ctctgttttc aaatgaccta atccagtttg ttccggagag agtaccgaaa
     1501 ttttgcactc tttcatccgt ggtgtatcaa acttcaacgt gctaaatgag acattaaata
     1561 tacatcaaca taatctaccg cactcatcgt taagaaaccg ggattcccat cagaatgagg
     1621 aatatcgtta aagcccgata aggagacggt taaacaaaat ggtgttaaat tacttgcttg
     1681 atttcgtttc acagctctgc tttctccact cgctcgccca ttcgaaatac gaccgtctgc
     1741 aaacacaaag gtaatgctcg ttacctcaga acgaacgcaa gatagcgtcc aatctccggt
     1801 tgatgtaccg gatacaatcg ccccctcaac atcaggtaaa tcaatgccat tggcaattaa
     1861 attttctcgc ccgattaata ctttaaacgg aaacggatct gtcacagcac cttgaatagg
     1921 tacacggcct aacaatgccg tcatcgcagt agaacccatt aaagtcgaat tttcgggaat
     1981 agtgtaataa ggtgtacgct ttatttgctg tatctgagta ttaccagtca tattttctgt
     2041 cccagcatta ttatttaatg ctgtcccagt atttgaactt tccccctctg ctttaaaagg
     2101 gcttggaaag ccggtttttg ttttcccgga ataattgcct gtaaccggtt taccttgttc
     2161 atcggttacc accatatcat caggatcgac ccagcgaatg ccattgttat ctacattccc
     2221 tgtttgtcct gctgattcag tcccaaagcc agaagaagcc cctcctacag ggattgcctc
     2281 tgcttctttc tgctgctgat taccactttg aacccgacct ttcagttctt cgatttggcg
     2341 attaaatcca tcccaagcag cagccaaacg gctttctaca cgactttcta cttcacccgc
     2401 aatacgagaa tccacttcgg cttctcttgc ttgtaagcgt aatacctctt gctcaaagcg
     2461 atcaccacgc tcatttgctt tacgagcttc ttcacgagta tcttttaatg ctcctaccaa
     2521 cgtttttaat gtatcgtgag gcgtatctcc tgcagtaata cctaatgtat tcgcctcctc
     2581 atcact
//
LOCUS       DANLXF010000267         2571 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.268, whole genome shotgun sequence.
ACCESSION   DANLXF010000267 DANLXF010000000
VERSION     DANLXF010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.268"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..1472
                     /gene="pepA"
                     /locus_tag="PXD66_002347"
     CDS             <1..1472
                     /gene="pepA"
                     /locus_tag="PXD66_002347"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HDL6320593.1"
                     /translation="VEKQRTACLVVGVYEPRRLCSAAEQLDKLSNGYISTLLRRGDLE
                     GKAGQTLLLHNVPNVLADRVLLVGCGKERELTERQYKQIIQKSVQTINETGSMEAVSF
                     LTELHVKGRTPYWNVRFAVEAIQEQLYAYNDFKSIKPEVRRELRRVIFNVAHRKDLPD
                     AERALEHGKAVALGISHAKNVANCPPNVCNPHYLAELAKGLAYDYPAISTTIVDEKQM
                     AELGMNAYLAVSRASENEAFLSVIEYKGNPNADAKPIILVGKGMTFDSGGLSIKPAEA
                     MDEMKYDMCGAATVYGTIKAIAEMKLPLNVIGLLAGCENAVDGKAYRPGDILTTMSGL
                     TVEVLNTDAEGRLVLCDALSYVERFEPELVIDIATLTGACMIALGNHNSGLMSPHNGL
                     ANELLAAAEQADDKAWRLPLGEEYQEQLKSNFADLANIGGRLGGACTAGQFLSNFTKK
                     YTWAHLDIAGTAWKSGAAKGATGRPVSLLTQFLINKAQV"
     gene            1646..>2571
                     /gene="cysK"
                     /locus_tag="PXD66_002348"
     CDS             1646..>2571
                     /gene="cysK"
                     /locus_tag="PXD66_002348"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003899275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDL6320594.1"
                     /translation="MTIYADNSYSIGNTPLVRLKNFGNGNIVVKVEGRNPSFSVKCRI
                     GANMVWQAEKDGILTKDKEIVDATSGNTGIALAYVAAARGYKLTLTMPETMSTERKRL
                     LRGLGVNLVLTEGAKGMKGAIAKAEEIAASDPDRYVTLKQFENPANPAIHQQTTGPEI
                     WDATEGKIDVLVAGVGTGGTITGVSRAIKQDKGKQIISVAVEPSESPVITQTLNGEEV
                     KPGPHKIQGIGAGFIPKNLDLSLLDRVEQVSSEEAITTARRLMAEEGILGGISSGAAV
                     AAADRIAKLPEFADKLIVVILPSASERYLSTLL"
BASE COUNT          786 a          531 c          589 g          665 t
ORIGIN      
        1 gtgttgagaa acaaagaacc gcctgtttgg ttgttggtgt ctatgaaccc cgccgattat
       61 gttctgccgc agaacagctt gataaattaa gtaacggcta tatcagcaca ttactgagaa
      121 gaggagattt agaaggcaaa gcagggcaaa ccttactctt gcataatgtg ccgaatgtac
      181 tcgctgatcg agtattatta gtcggctgtg gaaaagagcg tgaattaact gaacgccaat
      241 acaaacaaat tattcaaaaa agtgtacaaa ctattaatga aaccggttca atggaagctg
      301 tcagcttttt aaccgaactg cacgttaaag gtagaacacc ttattggaat gtgcgatttg
      361 cggtagaggc aatccaagaa caactatatg cctataacga tttcaaaagc atcaaacctg
      421 aagttcgccg tgaattacgc cgtgtgattt ttaatgtcgc acaccgtaaa gatcttcctg
      481 atgccgaacg agctttagaa cacggaaaag ccgtagcact tggaatcagc cacgctaaaa
      541 atgtagcaaa ttgtccgcct aatgtgtgta atcctcacta tttagccgaa ttggctaagg
      601 gtttagccta cgactatcct gccatttcaa ctaccattgt tgatgaaaaa cagatggcag
      661 aattgggaat gaatgcttac ttagcggttt ccagagcctc tgaaaatgag gcctttcttt
      721 cagtgattga atataaaggc aacccgaatg ctgatgcgaa gccgattatc ttagtcggca
      781 aaggaatgac ctttgactct ggcggtttat ccattaaacc tgcggaggcg atggacgaaa
      841 tgaaatacga tatgtgcgga gctgctactg tttacggtac aatcaaagca attgccgaga
      901 tgaaattacc actcaatgtt atcggcttac tcgccggctg tgaaaatgcg gttgatggca
      961 aagcctatcg accgggggat attctcacta caatgtctgg tttaaccgtt gaggtactca
     1021 acaccgatgc agaaggacgt ttagtgctat gtgatgctct cagctatgta gaacgttttg
     1081 aaccggaatt agtgattgat attgctaccc taactggtgc ttgcatgatt gccttaggta
     1141 atcataacag cgggctgatg tcacctcata acggtttagc aaacgaactc ttagctgccg
     1201 cagagcaagc cgatgacaaa gcgtggcgtt tacctttagg ggaagaatat caagaacagt
     1261 taaaatctaa ctttgctgat cttgctaata tcggcggacg tttaggcgga gcctgtactg
     1321 ccggtcaatt cctctctaac tttacaaaga aatatacttg ggcgcatcta gatattgccg
     1381 gcacggcttg gaaatctggg gcagccaaag gggcgaccgg cagaccggta tccttactaa
     1441 cacaattttt gatcaataaa gcccaggtct aactctcaca agcggtctga ttttatcgag
     1501 tttttgcaaa aagaaaatca gaccgatcct cttcatacgc ccctatattc tttttagtta
     1561 tatcaaataa attccagcta ctagaaaagc gattaaaaat aagtatgata aatcccaatc
     1621 tcaatactta ttaaggaata tcactatgac tatctatgca gacaactcat actcaatcgg
     1681 caataccccg ctggtgcgtt taaagaattt tggcaacggc aatattgtgg taaaagtgga
     1741 agggcgtaac ccaagtttta gcgtgaaatg ccgtattggt gcgaatatgg tgtggcaagc
     1801 agagaaagac ggtattctaa ccaaagacaa agaaattgta gatgcaacca gcggtaacac
     1861 tggaattgcc ttggcttatg tagcagccgc tcgtggttat aaattaacgc tcacaatgcc
     1921 ggaaacaatg agtaccgagc gtaaacgctt attgcgtggc ttaggagtaa atttagtctt
     1981 aaccgaaggg gcaaaaggaa tgaaaggagc gattgcgaaa gctgaagaaa tcgcggcttc
     2041 cgacccagat cgctatgtta ccttaaaaca gtttgaaaac ccggcaaacc cggcaattca
     2101 ccaacaaact accggtcctg aaatttggga tgcaactgaa ggtaaaattg atgtattagt
     2161 ggcaggcgta ggaacaggtg gcactattac cggtgtttcc cgtgctatta agcaagataa
     2221 aggcaagcaa attatttcag tggcggtaga accgagtgaa tcgccggtca ttacgcaaac
     2281 tttaaacggt gaagaagtca aaccgggccc gcacaaaatt caaggcattg gtgcaggctt
     2341 tattccgaaa aacttagatt tatcactgct tgaccgtgtt gaacaagttt caagcgaaga
     2401 agcgattaca accgcacgcc gtttaatggc agaagaaggc attttaggcg gtatttcatc
     2461 aggtgctgcc gttgctgccg cagatcgcat cgctaaacta cctgaatttg ccgataaatt
     2521 aattgtagtg attctgcctt cagcctcaga gcgttattta agtacgctac t
//
LOCUS       DANLXF010000268         2534 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.269, whole genome shotgun sequence.
ACCESSION   DANLXF010000268 DANLXF010000000
VERSION     DANLXF010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.269"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            142..2418
                     /gene="gshAB"
                     /locus_tag="PXD66_002349"
     CDS             142..2418
                     /gene="gshAB"
                     /locus_tag="PXD66_002349"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HDL6320595.1"
                     /translation="MKLQKLIRENHLALLFQQGNFGLEKESQRVDRNGNIVTTPHPAV
                     FGNRSYHPYIQTDFAESQLELITPPNAKLEDSFRWLSAIHEVVLRSLPEEEYIFPLSM
                     PAGLPPEEQIQEAQFDNPEDVQYRQHLSKNYGKYKQMVSGVHYNFQLSTEFVEKAFAL
                     QTEYSELKAFQNALYMKLANNFLRYQWILLYLLAATPTVESQYFYEKSPLARGQLVRS
                     LRSSPYGYVNSEKVVVNHDSLESYVESLEANVANGLLIAEKEFYSNVRLRGGKKAREL
                     LENGVKYAEFRLFDLNPFAPYGIELNDAKFIHYFLLGMLWLEETSGQKEVEIGKQKLY
                     QVALEDPRTETAFRAEGEAILNQLLAMLEELSAGQESLDLVREKLAQFADPSKTLNGR
                     LIAAIEEVGSYKALGAKLAQTYKAQAFERFYALSAFDNMELSTQALFFDLIQQGIEVE
                     VLDENDQFLALKFGDHLEYVKNGNMTSRDQYISPLIMENKVVTKKVLAKAGFNVPKSV
                     EFTNLEQAVAHYPLFEGKAVVIKPKSTNYGLGITIFQQAVKNREDFAKAVEIAFREDK
                     EVMVEDYLVGTEYRFFVLGDETLAVLLRVPANVVGDGIHSVKELVERKNDDPLRGDGS
                     RSPLKKIALGEIEQLQLKEQGLTVDSVPAKGQTVQLRANSNISTGGDSIDMTDQMHES
                     YRQIAVGIAQAMGAKVCGVDLIIPDLTKPAEPNLSSWGVIEANFNPMMMMHIFPYQGK
                     SRRLTKAVIKMLFPEIGN"
BASE COUNT          772 a          519 c          553 g          690 t
ORIGIN      
        1 ataaggggct ttatccgaag cagtatttct gttataacca aatcatctac ttcattccaa
       61 taaactaaaa cccactattt cactaagtct ttcatttgtg ttattgtgcc gatacatttt
      121 tttgactaca aaggatagat gatgaaacta caaaaactta tccgagaaaa tcatttagca
      181 ttgctgtttc aacaagggaa ttttgggctt gagaaagaga gccaacgggt tgatcgcaac
      241 ggcaatatcg ttactactcc ccaccctgcc gtattcggta atcgctctta ccacccttat
      301 attcaaaccg attttgccga aagtcagcta gagttaatca ctcccccgaa tgctaaatta
      361 gaagacagtt ttcgttggct ctctgctatt cacgaagtgg tattgcgttc gttgccagag
      421 gaggaatata tcttccctct cagtatgccg gcaggcttgc cgccggaaga gcaaattcaa
      481 gaagcacaat ttgacaaccc tgaagatgtg caatatcgcc aacacctttc caaaaactat
      541 gggaaatata agcaaatggt aagcggcgtg cattataatt ttcaactttc aacggaattt
      601 gtcgaaaaag cctttgcttt acaaacggaa tatagcgagc tgaaagcctt ccaaaacgca
      661 ctttatatga agttagctaa taactttttg cgttatcaat ggattttgct ctatttattg
      721 gctgccacgc ctacggtgga atcgcaatat ttttatgaaa aatcaccgct tgcgagaggg
      781 caacttgtcc gcagcttgcg ttcaagccct tatggttatg tgaattcgga aaaagtggtg
      841 gttaatcacg acagcttaga aagctatgtt gaaagccttg aagccaatgt tgccaacgga
      901 ttattaattg ccgaaaaaga attctattcc aatgtgcgtt tacgaggggg caaaaaagcc
      961 cgtgagctat tggaaaacgg tgtgaaatac gccgaattcc gtttatttga tttaaaccct
     1021 ttcgcccctt acggcattga gctgaatgat gccaaattta tccattattt cttactcggt
     1081 atgctttggc tggaggaaac aagcggtcaa aaagaggtcg aaatcggcaa acagaaactt
     1141 taccaagtcg cacttgaaga tccaagaaca gaaaccgctt tccgtgccga aggtgaagcc
     1201 attttaaatc aacttttagc gatgcttgaa gaattatctg ccggtcagga aagcctagat
     1261 ttggtgcggg aaaaattagc tcaatttgct gatccaagca aaaccctgaa cggacgttta
     1321 attgccgcaa tagaggaagt cggcagttat aaagcattgg gggctaaatt ggctcaaacc
     1381 tataaggcac aagcctttga gcgtttttat gcgttatcgg catttgataa tatggaactt
     1441 tccactcaag ccctgttttt tgatttgatc caacaaggga ttgaggtgga agtactggac
     1501 gaaaacgatc aattcctcgc cctaaaattt ggcgatcatt tggaatatgt gaaaaacggc
     1561 aatatgacca gccgagatca atacatttca ccgctgatta tggaaaacaa agtggtgacg
     1621 aaaaaagtat tggcaaaagc cggctttaac gtgccgaaaa gcgttgaatt taccaacctt
     1681 gaacaggctg tggcacatta tccgctattt gaaggcaaag cggtggtgat taagccgaaa
     1741 tcgactaact atggtttagg gattaccatc ttccaacaag cggtcaaaaa tcgagaagat
     1801 tttgcaaaag cggttgaaat tgcgttccgt gaagacaaag aggtgatggt ggaagactat
     1861 ttagtcggta ctgaataccg cttctttgta ctgggcgatg aaacccttgc ggtgttactg
     1921 cgtgtaccgg cgaatgtagt gggagatggc attcacagtg taaaagagct ggtggagcgt
     1981 aaaaatgacg atccgctacg tggcgatggc tctcgtagcc cgttgaaaaa aattgcctta
     2041 ggtgaaattg agcagttaca acttaaagag caaggcttaa ccgtagattc cgtaccggcg
     2101 aaaggtcaaa cagttcaact gcgtgccaat tccaacatca gcaccggcgg cgattcgatt
     2161 gatatgaccg accaaatgca cgaaagctat agacaaattg cagtgggaat tgctcaggca
     2221 atgggggcta aagtgtgtgg ggtggatttg atcattcctg atctcaccaa acctgccgag
     2281 ccaaatttaa gctcttgggg cgtcattgag gcaaacttca acccaatgat gatgatgcac
     2341 attttccctt accaaggcaa atcccgccgt ttaaccaaag cggtaatcaa aatgctgttt
     2401 cctgagatag gcaattaaat tttctctcta tacctctttt aaggagaatt tactcaagaa
     2461 ttctccttaa tttatttcta aagttttact ccaattaaat ttcactttgt gcaaaacact
     2521 ccaaaagaag aatt
//
LOCUS       DANLXF010000269         2515 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.270, whole genome shotgun sequence.
ACCESSION   DANLXF010000269 DANLXF010000000
VERSION     DANLXF010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.270"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            23..1000
                     /locus_tag="PXD66_002350"
     CDS             23..1000
                     /locus_tag="PXD66_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1852 domain-containing protein"
                     /protein_id="HDL6320596.1"
                     /translation="MTTNFKCQVHTIEFNEDYQPEQSTRLTTNFANLARGENRKQNLQ
                     NAIAMINNRFNDLANWDNPNADRYSVKLDIVSLDIDVEGKGEFFPAIEWLKTYVIDYK
                     TGKTIDGIIGNNFSSFVRDYDFSVLLLDHNKDKPQFSVPEGYGELHGKLFKHLLASPE
                     YTAKYKKPPVICLSVATTRTYTRTTNVHPILGVEYLQDSYSLTDAYFAKMGLKVRYFM
                     PPKSVAPYAFYFDENADLLNDYTNLELISTIATMEAFQKIYRPEIYNANSVAGQVYKG
                     SLTYQDYSVTKVEYDREERTQLAIKQGKYTEEHFIKRYQAILEAWATEE"
     gene            1088..2119
                     /locus_tag="PXD66_002351"
     CDS             1088..2119
                     /locus_tag="PXD66_002351"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDL6320597.1"
                     /translation="MSNILLPTSTAGSLPKPSWLAEPEKLWSPWKFEGEELLQAKRDA
                     LLVSLAEQIAAGIDIVSDGEQTRQHFVTTFIEHLEGVDFSKREIVRIRNRYDASVPSV
                     VGAVSRPKSVFVDDAKFLRAHTDKPIKWALPGPMTMIDTLYDGHYKSREKLAWEFAKI
                     LNEEAKELEAAGVDIIQFDEPAFNVFFDELNDWGVAALERAAEGLKCETAVHICYGYG
                     IKANTDWKQTLGNEWRQYEESFPKLQQSKIDIISLECQNSRVPMDLIELVRGKKVMLG
                     AIDVATNIIETPEQVADTLRKALQFVDADKLYPSTNCGMAPLSRQVAQGKLQALSQGA
                     AILRAEFAK"
     gene            2207..2428
                     /locus_tag="PXD66_002352"
     CDS             2207..2428
                     /locus_tag="PXD66_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320598.1"
                     /translation="MLNLFKKPIKKSSFEAWAKLMDDVAKVAILAIPVILYGEYSLLM
                     KFTHSITLFAVVYFLLATARLFRETAEEM"
BASE COUNT          773 a          542 c          566 g          634 t
ORIGIN      
        1 gaaaaataaa ggggacaata aaatgacaac aaattttaaa tgccaagtgc atactattga
       61 atttaatgaa gattaccaac cggagcaaag cacccgttta accaccaact ttgccaacct
      121 tgcccgtggc gaaaaccgca aacagaacct gcaaaatgcg attgcgatga tcaataaccg
      181 ttttaacgat ttagcgaact gggataaccc gaatgcggat cgttattcgg tgaaattgga
      241 tattgtctca ctggatattg atgtggaagg caaaggcgag tttttccctg cgattgagtg
      301 gctgaaaacc tatgttatcg actacaaaac cggcaaaacc attgatggca ttatcggtaa
      361 taacttctct tcttttgtgc gtgattacga tttcagcgta ttgttgttag atcacaataa
      421 agacaaaccg caatttagcg tgccggaagg ctatggcgag ttacacggca agctgtttaa
      481 gcatttattg gcatcgcctg aatataccgc aaaatataaa aaaccaccgg tgatctgttt
      541 aagcgtggca accacccgta cctacacaag aaccactaac gttcacccaa ttttaggtgt
      601 ggaatatctg caagatagtt attcattaac tgatgcctat tttgcgaaaa tgggcttaaa
      661 agtacgttac tttatgccgc caaaatccgt tgcaccctat gcgttctact ttgatgagaa
      721 tgcagattta ctcaatgatt acactaactt agagctaatc agcacgattg ccacaatgga
      781 agcgttccaa aaaatttacc gtccggaaat ttacaatgcc aattcggtcg cagggcaggt
      841 ctataaaggc agcctgactt accaagatta ttccgtcacc aaagtggaat atgaccgtga
      901 agaacgcacc cagttggcga tcaaacaggg taaatacacc gaagagcatt ttattaaacg
      961 ttaccaagcg attttagagg cttgggcgac ggaggaataa acgtagggtg ggcgtaagcc
     1021 caccataaaa tgagatataa ccaatggtgg gcttacgccc accctacttt ttataggaaa
     1081 attcaaaatg agcaacattc ttctcccaac ctctaccgcc ggcagcctac caaaaccttc
     1141 gtggcttgcc gagccggaaa aactgtggtc accttggaaa tttgaaggcg aggaactact
     1201 tcaagccaaa cgtgatgccc tccttgtttc ccttgccgaa caaattgcgg cagggattga
     1261 tattgttagt gatggcgaac agacccgcca gcactttgtg accaccttta tcgagcattt
     1321 ggaaggggtg gattttagca agcgtgaaat cgtgcgcatt cgtaaccgtt acgatgccag
     1381 cgtgccgagc gtggtggggg cagtttcccg cccgaaatcg gtgtttgtgg acgatgccaa
     1441 attcttgcgt gcgcataccg acaaaccaat caaatgggca ttacccggcc cgatgacaat
     1501 gatcgacact ctctatgacg gtcattacaa aagccgtgaa aaattggcgt gggaatttgc
     1561 caaaattctc aacgaagaag ccaaagagtt ggaagcggca ggggtggata ttattcaatt
     1621 tgacgaaccg gcatttaacg tcttcttcga tgagctgaac gactggggcg tggcggcgtt
     1681 agagcgtgcg gcggaaggct taaaatgcga aacggcggtg catatctgct acggctacgg
     1741 cattaaagcc aataccgact ggaaacagac cctcggcaac gaatggcgtc aatacgaaga
     1801 gagtttcccg aaactgcaac aatcaaaaat cgacattatc tcgttagagt gccaaaactc
     1861 ccgtgtgcca atggacttaa tcgaattagt gcgtggcaaa aaagtaatgc tgggagcgat
     1921 tgatgtggct accaacatta tcgaaacacc ggaacaagtg gcagatacgt tacgcaaagc
     1981 attacaattt gtcgatgcgg ataaacttta tccaagcacc aactgcggaa tggcaccact
     2041 ttcacgccaa gtcgcacaag gtaaattaca ggcactcagc caaggggcag cgatattgcg
     2101 tgcggaattc gcaaaataat tttgcgatct gcatcgaaaa tatcaacttt gctttttgct
     2161 cgtttccaga ttttcttcca aaataacagg aaatgacgga ggcactatgc taaatctatt
     2221 taaaaagcca attaagaaaa gctcctttga ggcttgggca aaactaatgg acgatgtggc
     2281 aaaagttgca attttagcaa ttcctgttat tctctatgga gaatattcac tgctaatgaa
     2341 atttacacac tcaattacct tatttgcagt agtttatttt ttgttagcaa cggcaagatt
     2401 gtttcgagaa acagcagagg agatgtaaat ggacggattg acaatcggat tatttctttt
     2461 gcttgcattt tcatttgctg cataccaatg ggcaaagcat ttaaaagatc ataat
//
LOCUS       DANLXF010000270         2507 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.271, whole genome shotgun sequence.
ACCESSION   DANLXF010000270 DANLXF010000000
VERSION     DANLXF010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.271"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            14..355
                     /gene="erpA"
                     /locus_tag="PXD66_002353"
     CDS             14..355
                     /gene="erpA"
                     /locus_tag="PXD66_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005706127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HDL6320599.1"
                     /translation="MSDIQIPLIFTDAAARKVKSLIEGEENPNLRLRVYITGGGCSGF
                     QYGFTFDDQVNEGDLTIENQSVGLVVDPMSLQYLIGGTVDYTEGLDGSRFVVNNPNAS
                     STCGCGSSFSI"
     gene            complement(460..783)
                     /gene="yidD"
                     /locus_tag="PXD66_002354"
     CDS             complement(460..783)
                     /gene="yidD"
                     /locus_tag="PXD66_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDL6320600.1"
                     /translation="MVKTHSPEQKAGTTESINQPPRLSLLAKILLLPIYFYRYAISPL
                     IGPRCRFYPTCSTYAVEAIKIHGAIKGGYLAFKRILRCNPLSEGGEDPVPLCCKGNCK
                     HNDKK"
     gene            complement(732..1106)
                     /gene="rnpA"
                     /locus_tag="PXD66_002355"
     CDS             complement(732..1106)
                     /gene="rnpA"
                     /locus_tag="PXD66_002355"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDL6320601.1"
                     /translation="MKKLNFSRELRLLAPVQFKAVFEEPLRASTPQMTLLARENNLEN
                     PRLGLTVAKKHLKRAHDRNRIKRIVRESFRLKQHELPNFDFVFVAKGGIGKLDNATLF
                     ETLEKLWSRHIRLSKKPAQQNP"
     gene            complement(1142..1276)
                     /gene="rpmH"
                     /locus_tag="PXD66_002356"
     CDS             complement(1142..1276)
                     /gene="rpmH"
                     /locus_tag="PXD66_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDL6320602.1"
                     /translation="MKRTFQPSVLKRARTHGFRARMATKNGRQVLARRRAKGRKSLSA
                     "
     gene            1513..2475
                     /gene="hemH"
                     /locus_tag="PXD66_002357"
     CDS             1513..2475
                     /gene="hemH"
                     /locus_tag="PXD66_002357"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HDL6320603.1"
                     /translation="MNQPQQIGILLANLGTPDEPTPSAVKRYLKQFLSDPRVIDLPKF
                     KWQAILNCIILPRRSPKVAKLYQSVWSEEGSPLLAISRQQQRAVQRYFDAKGKNVVVE
                     LGMSYGNPSIESATDNLIKQGVERIIVLPLYPQYSSTTTASVFDAFANGLKAQRKIVP
                     FDFIHSYHNDPLYIQALANSIDVKPDELLLFSFHGIPKRYETEGDFYAKHCQETAELV
                     AEKLGLPREKWYLSFQSRFGDEEWLQPYTDQTLESFPQKGIKKVAVVCPGFSADCLET
                     LEEITEENRENFEHAGGESYRYIPALNASGDHIKALVKLIEAKI"
BASE COUNT          756 a          484 c          550 g          717 t
ORIGIN      
        1 gatctaaaac aaaatgagcg atattcaaat tcctttaatc tttactgatg cggcggctcg
       61 taaagtaaaa agcttaattg agggcgaaga gaacccgaat cttcgcttgc gtgtgtatat
      121 tacgggtggg ggctgtagtg gattccagta tgggttcacc tttgatgatc aagtcaatga
      181 gggagacctt accattgaga atcaaagcgt tggcttggtg gttgacccga tgagcctaca
      241 atatttaatt ggtggcacgg tggattatac cgaagggctg gacggctcac gttttgtcgt
      301 gaataatcca aatgcaagct caacctgtgg ttgtggttct tcctttagca tttaattcac
      361 tcgaattatt gccttattca atatcagtct cacaagtaaa ttttttacta aaaatgaccg
      421 tttgtattaa acaaaatagc ctaacttatt gagtttaggc tattttttat cattatgttt
      481 gcagttgcct ttacaacaaa gtgggacagg atcttcgcct ccctcactca aagggttgca
      541 acgcagaata cgcttaaacg ctaaataccc acctttaatt gctccgtgga ttttaatcgc
      601 ttcgaccgca taggtggagc aggttgggta aaatcggcaa cgtgggccaa tcaatgggct
      661 gatagcatag cgatagaaat aaatcggtag caatagaatt ttggctaata ggctgagacg
      721 aggtggctga tttatggatt ctgttgtgcc ggctttttgc tcaggcgaat gtgtcttgac
      781 catagttttt ccaatgtttc aaacagcgtt gcgttgtcta atttgccaat gccgcctttt
      841 gccacaaata caaaatcgaa attcggcaat tcgtgttgct tgaggcgaaa actttcacgc
      901 actatccgct taatgcgatt acggtcgtga gcccgtttta aatgtttttt ggcaacagtt
      961 aagcctagac gaggattttc tagattattc tctctagcaa gaagagtcat ttggggtgtg
     1021 ctggctcgaa gtggctcttc aaacaccgcc ttaaattgaa cgggagctaa caaacgtagc
     1081 tcccgagaga aatttagctt cttcacactt aatcagaaag gtgttttggc tggttctgca
     1141 attatgcaga taaagattta cgacctttag cacgacgacg cgctaacact tgacgaccgt
     1201 ttttagtagc catacgagca cggaaaccgt gagtacgagc acgttttaat actgatggtt
     1261 gaaatgtacg tttcattgct tatctaccta aaatttaaaa agttggcata aaatggtttc
     1321 tgccaaacaa ggaccataaa aaagagttgc aattctacag gcaattaaaa taaaaagcaa
     1381 gaaacaatcc aagcacaact cgggttgttt gcaaaatttt tgaaaaatac gaccgcttgt
     1441 tataatcgca gaaatttata ttggtttgtg gcataatcga aaaaatatta actattgatg
     1501 agaaatagaa caatgaatca accccaacaa ataggtattt tgctggctaa tttaggtacg
     1561 ccagatgaac ccacgccaag tgcggtgaag cgttatttaa agcaattttt aagcgatcct
     1621 cgtgtgatcg atctaccaaa atttaaatgg caagcaatcc taaactgcat cattttgcca
     1681 agacgttcgc caaaagtggc aaaattatat caatcagtat ggagcgaaga aggctcaccg
     1741 ttattggcga tttctcggca acaacaacga gcggtacaac gctattttga tgccaaaggc
     1801 aaaaatgtgg tggtggaatt gggaatgtct tacggcaatc caagtattga aagtgcgacc
     1861 gataacctca tcaagcaggg ggttgagcgg attattgtgc taccgcttta tccgcaatac
     1921 agtagcacta ctaccgcttc ggtgtttgat gcgtttgcca acggtttaaa agcacagcgt
     1981 aaaatcgtgc cgtttgattt tattcacagc tatcataacg atccgcttta tattcaagcg
     2041 ttggctaact ctattgatgt taaacctgac gagttattac tgttttcatt tcacggcatt
     2101 cctaaacgtt atgaaacgga aggtgatttt tacgccaagc attgccaaga aacagcagaa
     2161 ttagtggctg aaaagctagg tctgccaaga gaaaaatggt atttgtcttt ccagtcccgt
     2221 tttggtgatg aagagtggct acagccttat accgatcaaa cccttgaaag cttcccacaa
     2281 aaagggataa aaaaagtggc tgtcgtttgc ccgggctttt cagcagattg tttggaaacc
     2341 ttagaagaaa ttacagaaga aaaccgcgag aactttgaac atgccggcgg agaatcttat
     2401 cgctacattc cagcgttaaa tgcgagtggc gatcatatca aggcattagt aaaattaatc
     2461 gaagcgaaaa tataatataa tttgggcgga taataaatcc gcccatt
//
LOCUS       DANLXF010000271         2497 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.272, whole genome shotgun sequence.
ACCESSION   DANLXF010000271 DANLXF010000000
VERSION     DANLXF010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.272"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            54..344
                     /locus_tag="PXD66_002358"
     CDS             54..344
                     /locus_tag="PXD66_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDL6320604.1"
                     /translation="MALRPLHDKVILKREEVETKSAGGIVLTGSAATKSTRGKVIAVG
                     TGRILDNSSVQPLAVKVGDVVIFNEGYGVKTEKIDGEEVLILAEHDILAIVE"
     gene            413..2053
                     /gene="groL"
                     /locus_tag="PXD66_002359"
     CDS             413..2053
                     /gene="groL"
                     /locus_tag="PXD66_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608625.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDL6320605.1"
                     /translation="MAAKDVKFGNDARVKMLKGVNILADAVKVTLGPKGRNVVLDRAY
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIVNE
                     GLKAVAAGMNPMDLKRGIDKAVAAVVEELKAISKPCETSKEIEQVGTISANSDSTVGQ
                     LIAQAMEKVGKEGVITVEDGTGLEDALDVVEGMQFDRGYLSPYFINKPEAGTVELENP
                     YILLVDKKVSNIRELLPVLEGVAKAGKPLLIIAEDIEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIAILTAGTVISEEIGMELEKATLEELGQAKRVVISKDNTTIID
                     GIGDETQIKGRVAQIRQQIEDSTSDYDKEKLQERVAKLAGGVAVIKVGAATEVAMKEK
                     KDRVDDALHATRAAVEEGIVPGGGVALVRAANKVADTLKGDNEEQNVGIKLALRAMEA
                     PLRQIVANAGEEASVVARNVKEGSGNYGYNAGTEQYGDMLEMGILDPTKVTRSALQFA
                     ASIAGLMITTECMITDLPKEEKLDPAAMGGMGGMGGMM"
     gene            complement(2178..2465)
                     /locus_tag="PXD66_002360"
     CDS             complement(2178..2465)
                     /locus_tag="PXD66_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5339 domain-containing protein"
                     /protein_id="HDL6320606.1"
                     /translation="MVSSNAIASVKTSSLTVAKPTLSKNLPTECEKMFNTANKLVSDA
                     ERQPGTHTQVLKMKNKLSLTKQQILKMDSELQQKSCEKGLTALNSLKQKHQ"
BASE COUNT          791 a          407 c          605 g          694 t
ORIGIN      
        1 tacgcatttt gcgtgaagtt ttatttattc atttttaaga aaaggaacat attatggcac
       61 ttcgtccatt acacgataaa gttattttaa aacgtgaaga agttgaaaca aaatcggcag
      121 gcggtattgt tttaaccggt tcggcagcaa caaaatcaac ccgtggcaaa gtaattgcag
      181 taggaacagg tcgcatttta gacaatagtt ctgttcagcc tttagcagtt aaagtgggcg
      241 atgtagtgat ttttaacgaa ggctacggcg taaaaaccga gaaaattgac ggcgaagaag
      301 tgctaatttt agcagagcac gatattttgg cgattgtaga ataatttcgt tctgcacaag
      361 cggttaaaaa agcaaaattt tttacatatt tcaaatacaa aggatttaga aaatggcagc
      421 aaaagacgtg aaatttggta acgatgcacg cgtaaaaatg ttaaaaggcg tgaatatttt
      481 agccgatgcg gtaaaagtaa ctttaggtcc gaaaggtcgt aatgtggtgt tagatcgtgc
      541 ttatggtgca ccaacgatta caaaagacgg tgtgtcggtt gcccgtgaaa tcgaattaga
      601 agataaattt gaaaatatgg gggctcaaat ggtgaaagag gttgcctcta aagccaatga
      661 tgccgccggt gacggtacaa ctaccgcaac tgtattagct caagcgattg taaacgaggg
      721 cttaaaagca gtggctgcgg gtatgaaccc aatggattta aaacgtggta tcgataaagc
      781 ggttgcagca gtggttgaag agttaaaagc gatttcaaaa ccgtgcgaaa cctcaaaaga
      841 aattgagcaa gtagggacga tttctgcaaa ctcagattca acagtcggtc aattaattgc
      901 tcaagcaatg gaaaaagtgg gcaaagaggg cgtgattact gttgaagacg gtacaggttt
      961 agaagatgca ttagacgtgg ttgagggtat gcaatttgac cgtggttact tatcgccata
     1021 ctttatcaat aaaccggaag ccggtacggt tgagttagaa aacccatata ttcttttagt
     1081 ggataaaaaa gtgtctaaca ttcgtgaatt attaccggtg ttagaaggtg ttgcaaaagc
     1141 aggtaaaccg ttattaatta ttgccgaaga tattgaaggc gaggcgttag ctaccttagt
     1201 ggtgaacact atgcgtggta tcgtgaaagt ggcggcagtg aaagcacctg gctttggtga
     1261 tcgccgtaaa gcaatgttac aagatattgc gatcttaact gcaggtactg tgatttctga
     1321 agagatcggt atggagcttg aaaaagcaac ccttgaagag cttggtcaag caaaacgtgt
     1381 ggttatcagc aaagataaca ccacaattat tgacggtatt ggtgatgaaa ctcaaatcaa
     1441 aggtcgtgta gcacaaatcc gccaacaaat cgaagattca acttcagatt acgacaaaga
     1501 aaaactacaa gagcgtgtag caaaattagc cggtggtgtt gcggtaatta aagtaggtgc
     1561 ggcaactgaa gtggctatga aagagaaaaa agaccgtgtt gatgatgctc ttcacgcaac
     1621 ccgtgctgcg gtggaagaag gtattgtacc ggggggcgga gttgctttag tgcgtgcagc
     1681 caacaaagtg gctgatacac taaaaggcga taacgaagag caaaatgtag gtatcaaact
     1741 tgccttgcgt gcaatggagg ctccattacg ccaaatcgtt gccaatgcag gtgaagaagc
     1801 ctcagttgta gcccgcaacg tgaaagaagg ttcaggcaac tacggctata acgcaggtac
     1861 agagcagtac ggcgatatgc ttgaaatggg aattttagac ccaactaaag tgactcgttc
     1921 agcacttcaa ttcgcggctt ctattgcagg tttaatgatc actacagaat gtatgattac
     1981 cgatctgcca aaagaagaga aattagatcc tgccgcaatg ggcggtatgg ggggaatggg
     2041 cggaatgatg taattgccca ttgagtgaac aaaagctact gaaattaatt cagtagcttt
     2101 tttgttggta acaagtggtt aaatttatgc tattttttgc aaataaaaaa ccaagcaagt
     2161 aagcttggtt ttagcgttta ttgatgtttt tgtttaaggc tgtttagtgc tgttaaacct
     2221 ttctcacaac ttttttgctg taattcagaa tccattttta ggatttgttg ctttgttaaa
     2281 gaaagtttat ttttcatttt tagcacttga gtatgagtac caggctgtct ttctgcatca
     2341 gagaccaatt tatttgccgt gttaaacatt ttctcacatt cagttggcaa atttttactt
     2401 aaagtaggtt ttgcaactgt aagtgatgag gttttaacac ttgctatagc attactggaa
     2461 accaatatgg tacaaagtgt tattcctaaa attcttt
//
LOCUS       DANLXF010000272         2458 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.273, whole genome shotgun sequence.
ACCESSION   DANLXF010000272 DANLXF010000000
VERSION     DANLXF010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.273"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            103..516
                     /locus_tag="PXD66_002361"
     CDS             103..516
                     /locus_tag="PXD66_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
                     /protein_id="HDL6320607.1"
                     /translation="MKNEHNLEQLKYIWDNRNLENKVYQYLFHICIEPMKKGDWNRYT
                     IWLNFHGNLINVSDVVYRVVESQKLKDDIKIWYDKQYYCRPTKVETTGFISIFLHLFY
                     EGIARILLEQGMITETEAAEKSYNFRAANCFQRLL"
     gene            530..685
                     /locus_tag="PXD66_002362"
     CDS             530..685
                     /locus_tag="PXD66_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320608.1"
                     /translation="MEPRTVKVIYDDGDYFFTLINADEQTIRNYYAIGSSIGFDNTRK
                     IVAVEIL"
     gene            765..1748
                     /locus_tag="PXD66_002363"
     CDS             765..1748
                     /locus_tag="PXD66_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1738 domain-containing protein"
                     /protein_id="HDL6320609.1"
                     /translation="MNPQSNTQPKFDLYQEVTDKIIAALEQGTAPWLKPWDSVIPYGG
                     MPCNAISGRLYSGINVLLLWLSAAEHGYIQRKWITAKAAIEAGGHVRKGEKATLAVSY
                     RPYDREKTDDDGNLVLDDDGNPVIEHLASIKKHCLFNIEQCEKLPQDWYETVETPEQL
                     TDENQYAIFEEIRLMLKGMDLQVMVKPSNQAYYHPRNDHIVMPEMKQFHSPQKFHSVL
                     LHEMTHATGHQSRLARDGIVSGKARFGNKVYAFEELVAEMGCAFLCSHLGFADVPQNA
                     SYIESWIKVLREDKRAIFRASGKAREACQYMLDALNIAQLADKYEVFEEAA"
BASE COUNT          752 a          468 c          508 g          730 t
ORIGIN      
        1 tactttaatt tgaccgcttg tagtggttaa ttttcacccc gtggggaaat attttcccta
       61 ttgggcaaaa tgtttctcca ctttttaatt tggagaaatg ttatgaaaaa tgaacacaat
      121 ttagagcaac ttaaatatat ttgggataac cgcaaccttg aaaataaagt ctatcaatat
      181 ctgtttcaca tctgcattga gccgatgaaa aaaggagatt ggaatcgtta tacgatttgg
      241 cttaattttc acggtaactt aatcaacgtg tcagacgttg tatatcgagt tgtggaatct
      301 caaaagctca aagatgacat caaaatttgg tatgacaagc aatactattg ccgtccaacc
      361 aaagttgaaa caacaggttt tatttccatt tttctgcacc tgttttatga gggcatagct
      421 cgtattttgc tggaacaggg aatgattact gaaactgaag cagccgagaa aagttacaac
      481 ttcagagctg caaattgttt tcaacgtttg ctttaatgga ggtgagataa tggaacctcg
      541 cactgttaaa gttatttacg atgatggcga ttatttcttt accctaatca atgccgatga
      601 acagactatt cgtaattact acgctatagg cagttcaatc ggttttgata acacaaggaa
      661 aattgtcgct gttgaaattt tataaactac ccatcgggga aacgacttcc ccatagggcg
      721 atagtgtttc cccatttttc tacccgaact ttggagaaac acttatgaac cctcaatcaa
      781 acacacagcc taaatttgat ttatatcaag aagtgaccga taaaatcatc gccgctttag
      841 agcaaggaac ggctccttgg cttaaacctt gggatagcgt tattccttat ggtggtatgc
      901 cgtgtaatgc catatccgga agattgtatt ccggaatcaa tgttttgtta ctttggttgt
      961 ctgccgcaga acacggctat atccaacgca agtggatcac tgcaaaagca gctattgaag
     1021 ccggtggaca cgttcggaaa ggtgaaaaag caacccttgc ggtcagctat cgcccgtatg
     1081 atcgagaaaa aaccgatgat gatggcaacc ttgtcctcga tgacgatggt aacccggtaa
     1141 tcgaacatct cgcttcgatt aaaaagcact gcctattcaa tattgaacag tgcgaaaaac
     1201 tgccacagga ctggtatgaa acggttgaaa ccccagaaca gctgactgac gaaaatcagt
     1261 atgctatttt tgaggagatt cgtttgatgt tgaaaggtat ggatttgcaa gtgatggtaa
     1321 aaccaagcaa tcaagcttac taccacccac gcaatgacca tatcgtgatg ccggaaatga
     1381 agcagtttca ttcaccacaa aaattccata gcgtgctgct acatgaaatg actcacgcaa
     1441 cagggcatca atctcgcctt gctcgtgatg gtattgtttc cggaaaagct cgctttggca
     1501 ataaagttta tgcttttgaa gaattggtcg ctgaaatggg ctgtgcattc ttgtgttctc
     1561 acctaggctt tgctgacgtg ccacaaaatg ccagctacat tgaaagttgg atcaaggtgt
     1621 taagagagga taaacgggca attttcaggg cttcagggaa agctcgggaa gcctgtcaat
     1681 atatgctgga tgccttaaac atcgcacagc tggctgataa atacgaagta tttgaagaag
     1741 ccgcttaata aatttgccga aaggcatttc ccgtggggag atactctccc ttcggggcat
     1801 tgtgtctccc tttttattca cttaacgaga ggactcaatg ctcaatctaa acttatttaa
     1861 acgacccatt gaaatttcga cacttgaatc ttggtcaaaa ttctgtgatg atgttggtaa
     1921 agtagctttt gttgcagtac ctgctatgct gtatagtaat tacgagtttt ggattaagat
     1981 aagcagtata attggtctat ttatagctac ctatttctgt ttagttatcg gtagaaatat
     2041 acgccaattt attgagaagg agaaatcaaa atgggattaa tgatcggcat ttggattatt
     2101 gcttttattg catttgggtt ggccatatgg acaagccata ttctaaaaaa gcaagatgaa
     2161 cgccttaaac agctaaaaaa tagctaactt tcaaaatacg gcacattttg tgccgtattt
     2221 cttttgtact ggtggcaaat ataagattac tatcatcagt atattgtaat atctcaactc
     2281 aacagcatta gtcaaaaatc ctccaacata ttcagagagt ttttcctact gagttctctt
     2341 attttttggt tcaatactct ccctttataa attaaatttg gttcagataa aagccgctca
     2401 atggcaaaag gataaaggag cccttacggg caagggtaat cccctaaggg ggaataag
//
LOCUS       DANLXF010000273         2444 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.274, whole genome shotgun sequence.
ACCESSION   DANLXF010000273 DANLXF010000000
VERSION     DANLXF010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.274"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(116..191)
                     /locus_tag="PXD66_002364"
     tRNA            complement(116..191)
                     /locus_tag="PXD66_002364"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(156..158),aa:Thr,seq:ggt)
     gene            complement(195..269)
                     /locus_tag="PXD66_002365"
     tRNA            complement(195..269)
                     /locus_tag="PXD66_002365"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(234..236),aa:Gly,seq:tcc)
     gene            complement(313..397)
                     /locus_tag="PXD66_002366"
     tRNA            complement(313..397)
                     /locus_tag="PXD66_002366"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(361..363),aa:Tyr,seq:gta)
     gene            complement(432..507)
                     /locus_tag="PXD66_002367"
     tRNA            complement(432..507)
                     /locus_tag="PXD66_002367"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(472..474),aa:Thr,seq:tgt)
     gene            754..1704
                     /gene="coaA"
                     /locus_tag="PXD66_002368"
     CDS             754..1704
                     /gene="coaA"
                     /locus_tag="PXD66_002368"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDL6320610.1"
                     /translation="MSLEKTSKITPFLTFDRQQWADLRKSVPLKLTEQDLKPLLGFNE
                     DLSLEEVRTIYLPLARLIQYYIEENLKRQTVLHRFLGIEPPKVPYIISIAGSVSVGKS
                     TSARILQALLSHYPKERKVDLITTDGFLYPLATLTEKNLLKKKGFPESYDIHSLIRFV
                     SDIKSGKRNVKAPIYSHLTYDIIPNQFNTVDQPDIVILEGLNVLQSGMNYPSSPHNVF
                     VSDFVDFSIYVDAEESLLKKWYINRFLKFRRSAFADPNSYFHHYASLSEQEAIETAST
                     IWEEINGLNLRQNILPSRERANLILIKGQDHAIETVKLRK"
     gene            1857..2396
                     /locus_tag="PXD66_002369"
     CDS             1857..2396
                     /locus_tag="PXD66_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HDL6320611.1"
                     /translation="MNKKFLLPLILFLALSVAFLVQLARNSQGDDPKKLESALIGKTV
                     PSFNLESVLDEKQTIDQNIVKTGKPRLLNVWATWCPTCYAEHEYLTILAKQGVEIVGI
                     DYKDERPKAIKFLANYGNPYKAIIFDPKGSLGLDLGVYGAPETFIIDGNGVIQYRHAG
                     DVNDQVWNQVLKPIYDGLK"
BASE COUNT          766 a          510 c          466 g          702 t
ORIGIN      
        1 tttctaaaag cctcttaaac aaaaacggtt acaatggaac aaaagaaccc attaaccata
       61 atgtagaaat taataccaat aaaaatttgc gtgagaaaga tagaattcag agaagtggtg
      121 ctgataggcg gatttgaacc gccgacctca cccttaccaa gggtgcgctc taccaactga
      181 gctatatcag cgcttggagc gggcagcggg aatcgaaccc gcatcattag cttggaaggc
      241 taaggtaata gccattatac gatgcccgct gttctaaatt cgtctgacct acgcgattca
      301 aaatatcaga gatggtggag ggagaaggat tcgaaccttc gaaggctgag ccaacagatt
      361 tacagtctgc cccctttggc cgctcgggaa cccctccacg ataattgaat acttatttac
      421 gcctgagtta atggtgccga ctaccggaat cgaactggtg acctactgat tacaagtcag
      481 ttgctctacc tactgagcta agtcggcgtt gctgtgtcgt aagcaagtga tgtgcattat
      541 agggaaattt taacagcgtg caagtgtttt ttaaaaaaat attaaaaaaa gcgttctttc
      601 gatttttaaa tatacaaaat gactattttt tgtaaaatac aacggatttt ctctgcataa
      661 ctacggcaaa ataagtgcat atattgtata gttacgccct taatttggta tttaattacc
      721 ttaatattcc agcaaaaaat tgagattttg gctatgagcc ttgaaaaaac cagcaaaatt
      781 actccttttc tcacttttga tcgccaacaa tgggctgatt tacgcaaatc cgttccattg
      841 aagctgaccg agcaagacct aaaaccgctt ttaggcttta atgaagatct ttcattagaa
      901 gaagtacgca ctatttattt accgcttgcc cgtttaattc aatactatat agaagaaaac
      961 ttaaaacgcc agacagtctt acatcgcttt ctcggcattg aaccgccaaa agtcccttac
     1021 attattagta ttgccggcag cgtatcggtt gggaaaagta cctctgcccg tattctgcaa
     1081 gctctgctct cccattaccc taaagagcga aaagtggatt taattaccac cgacggtttt
     1141 ttatatccac ttgcaacgct aaccgaaaag aacttactca agaaaaaagg ctttccggaa
     1201 tcttatgata ttcacagcct gattcgcttt gtatctgata ttaaatccgg caaacgcaat
     1261 gtgaaagctc cgatttattc acatttgacc tacgatatta tcccaaatca atttaatacg
     1321 gtggatcaac ccgatattgt gattttagaa gggctaaacg tgttacaaag tggaatgaac
     1381 tacccgtcca gcccacataa tgtatttgta tcggactttg tggatttttc tatttatgtg
     1441 gatgccgaag aaagtttgct gaaaaaatgg tatatcaacc gtttcttgaa attccgccgc
     1501 agtgcctttg ccgacccaaa ctcctatttc caccattatg cgagtttgtc cgagcaagaa
     1561 gcgattgaaa ccgcctcaac gatttgggaa gagatcaacg gcttaaattt acgccaaaat
     1621 attctcccaa gccgagagcg tgccaacctc attttaatca aagggcaaga ccacgccatt
     1681 gaaacggtaa aattacgcaa ataatatttt gcaaataatt gggctttttc gaccgcttgt
     1741 aataccttaa aatccgttga ataccactca acggattttt tatttaaatc aatgcttatt
     1801 caactatttt ttggtaagct aacgactttg agagtgattg atattaggca tatacaatga
     1861 acaaaaaatt tctacttcct ttaattctct ttttggcgtt atctgtggca tttttggttc
     1921 aactcgctcg caactcgcaa ggcgatgatc cgaaaaaatt agaatcagcc ttaattggca
     1981 aaacagttcc gagctttaat ttagaatcgg tattagatga aaaacagaca atcgaccaaa
     2041 atatcgtcaa aaccggcaaa cctcgcctgc ttaacgtgtg ggcaacttgg tgtccgactt
     2101 gttatgccga acacgaatac ttaaccattt tggcaaaaca aggggtggaa attgtcggta
     2161 ttgattataa agacgaacgc ccaaaagcga ttaaattctt agccaattac ggcaaccctt
     2221 ataaagcgat tatttttgac ccgaaaggct cactcggttt agatttaggt gtttatggtg
     2281 cacccgaaac ctttattatt gacggcaacg gcgtgattca ataccgccac gccggcgatg
     2341 taaacgacca agtgtggaac caagtgctta aaccgattta tgatggatta aagtaaatgt
     2401 ttgtatttcc tgctgacatt aagaaatcta taaaaagcaa ttat
//
LOCUS       DANLXF010000274         2441 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.275, whole genome shotgun sequence.
ACCESSION   DANLXF010000274 DANLXF010000000
VERSION     DANLXF010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.275"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..1291)
                     /locus_tag="PXD66_002370"
     CDS             complement(<1..1291)
                     /locus_tag="PXD66_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HDL6320612.1"
                     /translation="MIYAEIFVVLAAIYLGLRSGGLGIGLYGGLGLAILTLGFGLPMG
                     SIPVDVMLIIMTVVIGAAVLQAVGGMDLLVRYAEVLMRKNPKYINFIAPMITWLMTIM
                     AGTGFIVFSTLPVIAEVAKESGIRPSRALAGSVVSSQLAIAGSPLSAAMAAMIAVMEN
                     NSVTFFQVIAVCLPVSFIASMVAAFVASKQGCELQNDVVYLERLKAGIVSPSRKLASE
                     STYSKGAKLSIGIFLLATVLVVLLAAVPGLKPVYENGKSMSTRDIIIVMMLASSCLMM
                     LVGKIKPDTIVASPIFRSGMTSIAVIIGIVTLGMTFVDAHLIEIKASIGNILTEYPML
                     LAVVLFFTCALLYSQGSTSALIIPLAVSLGIPNWAILASFIAITGIFILPTYPTSLAA
                     MELDTTGSTRAGKYIIDHPFMLPALVGVAVGLAFGFVW"
     gene            complement(1307..2002)
                     /locus_tag="PXD66_002371"
     CDS             complement(1307..2002)
                     /locus_tag="PXD66_002371"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HDL6320613.1"
                     /translation="MARNIIGILGGMGPAATADMFQKFIRLTPADCDQAHIPLLISSI
                     PDIPDRTRYILANGENPAPVMEKYIEGLESAGATCIIIACNTAHYWFEHLKAKSQVEM
                     LSMIDCVVDEVLKCGKWRVGLLATDATLAVDLYKKQLEAQGLTFICPTENGQKAVMDS
                     IYLLKSGELEKATELMLQQRDELIRLGAEVIILGCTEVPIILAQEIEREPMRYVDSTL
                     VLVKKAIDWYQTH"
     gene            2218..>2441
                     /locus_tag="PXD66_002372"
     CDS             2218..>2441
                     /locus_tag="PXD66_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL6320614.1"
                     /translation="MKNIETKWLEDFLTLEECRHFSQAAEKRNLSQSAFSRRILALEE
                     VVGVKLFDRTSIPLQLTEQGKLFHSQTRNL"
BASE COUNT          850 a          518 c          387 g          686 t
ORIGIN      
        1 cccatacgaa tccaaaagca agcccaacag caaccccaac taaagctgga agcataaacg
       61 gatgatctat aatatatttg ccagcacgag ttgaacccgt agtatctaat tccatcgctg
      121 ctaatgaagt tgggtaggtt ggcaggataa aaatgcctgt aattgcaatg aaagaagcca
      181 aaattgccca gttagggatc cccaagctta ctgctaatgg aataataaga gcagaagttg
      241 acccttgtga ataaagtaat gcacaagtaa aaaagagtac tacagctaac aacattggat
      301 attcagttaa aatatttcca atagaggctt taatttctat taaatgtgca tcgacaaaag
      361 tcatacctaa tgtaacaatc ccgataatta ccgcaataga agtcattcct gagcggaaaa
      421 ttggactagc cacaatcgta tcaggtttaa tttttcctac caacatcatt aagcacgaag
      481 aggctaacat catcacaata attatatctc gggtactcat tgacttccca ttttcatata
      541 ctggctttaa ccctggaacg gctgcaagta gaacaaccaa tacagttgct agtaagaaaa
      601 tgccaatcga tagttttgct ccttttgaat aggtagattc actcgctaac ttacgagatg
      661 ggctgacgat tcctgctttt aaacgttcta aataaacaac atcattttgc aattcacagc
      721 cctgcttaga agcaacaaat gcagcaacca tagaggcaat aaaagaaaca ggtaagcata
      781 ctgctattac ttgaaaaaat gtaacactgt tgttttccat tactgctatc atagccgcca
      841 ttgcagcact caatggagaa cctgcaatag ctaattgaga agacacaacc gagccggcta
      901 atgctcttga agggcgaata ccactctctt ttgccacttc tgcaatcaca ggtaaggtgg
      961 aaaagacgat aaaacctgta ccagccataa tcgtcattaa ccaagtaatc atcggtgcaa
     1021 taaagttaat atatttagga tttttacgca tcaatacttc cgcataacga acaagtaaat
     1081 ccatacctcc tactgcttgt aacacagcag cacctattac aacagtcata ataatcagca
     1141 ttacatcaac tggaatagaa cccataggta aaccaaaacc tagagttaga atcgcaagcc
     1201 ctaaaccacc atatagtcct attcctaagc ccccagaacg cagtcctaaa tagattgcag
     1261 caagtaccac aaagatttct gcataaatca tatccatctc cttaaattaa tgagtttgat
     1321 accaatcaat tgctttttta accaatacca acgttgaatc tacataacgc attggctcac
     1381 gctctatttc ttgagctaaa ataataggta cttctgtaca acctaaaata attacttctg
     1441 cacctaaacg aatgagttca tcacgttgtt gtaacattag ctctgtggct ttctctaact
     1501 cacctgattt taataaatag atactatcca ttactgcctt ttgaccattt tctgtcgggc
     1561 aaataaaagt cagtccttgt gcttcaagct gttttttgta taggtctacc gccaaagttg
     1621 catcagttgc caacaatcca acccgccatt ttccacactt caaaacctca tcaaccacac
     1681 aatcaatcat acttagcatc tctacctgac ttttggcctt caagtgttca aaccaataat
     1741 gtgcagtatt acatgcaata atgatgcagg ttgcccctgc actctcaagc ccttcaatat
     1801 atttttccat taccggagcc ggattctctc catttgctaa aatatagcga gttctatctg
     1861 gtatgtcggg aatagaggaa atcaataatg gaatatgagc ttgatcacaa tcagctggtg
     1921 ttaagcggat aaatttttgg aacatatctg ctgttgctgc aggtcccatt ccacctaaaa
     1981 tgccaataat atttcttgcc atacacttct ccttataaga tttaatttac tgcgatgact
     2041 tatacaaaca agttatgcaa aaaagaaatg caataaatcg aatcagtatg caaaaaatgc
     2101 aacaaagctc tttatagata gaaaaaaaat ctttaaaatc aataaaatac taatatatca
     2161 aatataaatt taatgcattt aatgaaatca taatgcgaaa tttgcattgg ttaggatatg
     2221 aaaaacatag aaacaaaatg gttagaagat ttcttaacgc tagaagaatg tcgccatttt
     2281 tctcaggcag cagaaaaaag aaatttgtca caatctgcat ttagcagacg tattttagct
     2341 ttagaagaag ttgtgggagt gaagttattt gatcgtacct ctattccatt acaactcaca
     2401 gaacaaggta agctatttca ttcacaaacg cgaaacttat t
//
LOCUS       DANLXF010000275         2439 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.276, whole genome shotgun sequence.
ACCESSION   DANLXF010000275 DANLXF010000000
VERSION     DANLXF010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.276"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            23..1090
                     /gene="hisC"
                     /locus_tag="PXD66_002373"
     CDS             23..1090
                     /gene="hisC"
                     /locus_tag="PXD66_002373"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDL6320615.1"
                     /translation="MTISQLSRKNIQALTPYQSARRLGGSGDVWLNANEYALSPNFDL
                     TDRTFNRYPEPQPQAVIEGYARYAGVAPENVLVSRGGDESIELIIRAFCEANDSILYC
                     PPTYGMYAVSADTCGIATKTVPLTADFQLDLAQIKANLEGVKVVFVCSPNNPTGNLIK
                     RSDLFELLQITAGKAIVVVDEAYIEFCPEARLVSELANFPHLAIIRTLSKAFALAGLR
                     CGFTLANAELIGVLQKVIAPYPLPVPVSDIAAQALSPQGIEQMKTRVAEVITLRAELQ
                     KNLENLPLVEQVFDSEANYLLFKCQDGQKVFKALWEQGIILRDQHKALGLQNCIRITV
                     GAREENLRVVEAITALSQFQR"
     gene            1275..2363
                     /gene="hisB"
                     /locus_tag="PXD66_002374"
     CDS             1275..2363
                     /gene="hisB"
                     /locus_tag="PXD66_002374"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018355873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HDL6320616.1"
                     /translation="MQPTLFIDRDGTLIDEPKTDFQIDSLEKLKFEKNVIPALLKLKG
                     KYRFVMVTNQDGLGTSSFPQADFDKPHNAMMALFNSQGIEFDDVLICPHKPEDGCDCR
                     KPHTKLLQKYIDRQLFDPANSFVIGDRATDVQLAENLGIRALQYHPEKLNWDLIAEKL
                     LGEAVTNIGERPKRFAEVIRKTKETDIKVQVWLDETGNNEISTGVGFFDHMLDQIATH
                     GGFRMNITTKGDLWIDEHHTVEDTALALGTALKQAIGDKRGIARFGFVLPMDESRAEC
                     AMDLSGRPFIKFKADFKRDKVGDFSTELTEHFFQSIAFTMLATLHIKAKGDNDHHKIE
                     SLFKVFGRTLRQCIRIEGNELPSSKGVL"
BASE COUNT          682 a          617 c          592 g          548 t
ORIGIN      
        1 ataaattata caggaacaca atatgacaat ttcacaactt tcccgtaaaa acattcaagc
       61 tctcacccct taccaatcgg cacggcgttt ggggggtagt ggcgatgtgt ggctgaatgc
      121 caatgaatat gccctttcgc caaactttga tttaaccgac cgcactttta accgctaccc
      181 tgaacctcaa ccgcaggcgg tgattgaagg ttacgcccga tatgcaggcg ttgcacctga
      241 aaacgtgctg gtttcccgtg ggggcgatga gagcattgag ctgattatcc gagccttttg
      301 cgaggcaaac gacagcattc tctactgccc accaacctac ggaatgtacg ccgtgagtgc
      361 cgacacctgt ggcattgcga ccaaaactgt gcctttaacc gccgatttcc agttggattt
      421 agcccaaatc aaagctaacc tcgagggcgt gaaagtggtg tttgtgtgca gcccgaacaa
      481 cccgacaggc aacttaatca agcggtcgga tttgttcgaa cttttgcaaa tcaccgcagg
      541 caaagcgatt gtggtggtgg acgaagccta catcgaattt tgccctgaag ctcgcttggt
      601 aagcgagctg gcgaatttcc cacatttggc gattatccgc accctatcca aagcctttgc
      661 cttggcgggt ttgcgttgtg gctttacctt ggcaaacgct gagctgatcg gcgtgttaca
      721 aaaagtgatt gccccgtatc cgctgcctgt gcctgtttcc gacatcgccg cccaagccct
      781 ctccccacaa ggcattgagc agatgaaaac ccgtgtggcg gaagtcatca ccctgcgtgc
      841 cgaattgcaa aaaaatctgg aaaacttacc gcttgtagaa caagtttttg acagcgaagc
      901 caactacctc ctattcaaat gccaagacgg acaaaaagtc ttcaaagcac tttgggagca
      961 aggcattatt ttgcgtgacc aacacaaagc gttaggattg caaaattgca ttcggattac
     1021 ggtgggggcg agagaggaga atttgagggt ggttgaggcg attacagcac tgagccaatt
     1081 tcagagatag aatttaacga gcagtatttt aagagacatt tatagcctag cacggcatag
     1141 ccgtactagg ttacgcataa gtcagtccct ttgggactga atacagagag gcaaagtctc
     1201 tcactaatgc ttaaccccat atgccttggc gtgtggggag agtaatgaat aagatacaga
     1261 tacaggagaa accaatgcaa cccacccttt ttatcgaccg tgacggcacg ttaattgatg
     1321 agccaaaaac cgattttcaa atcgacagct tggaaaagct gaaattcgag aaaaacgtga
     1381 ttcctgccct gctcaaactc aagggcaaat accgctttgt gatggttacg aaccaagacg
     1441 gcttaggcac gagttcgttc ccacaggcag atttcgacaa accgcacaat gcaatgatgg
     1501 cgttgttcaa ctcgcaaggg attgaatttg atgatgtgct gatttgtcca cacaagcccg
     1561 aagacggttg cgattgccgc aaaccgcaca ccaaactgct acaaaaatat atcgaccgcc
     1621 aactgttcga cccagccaac agctttgtga ttggcgaccg tgctaccgat gtgcaactgg
     1681 cggaaaacct tggcattcgg gcgttgcaat atcaccctga aaaactgaac tgggatttga
     1741 ttgctgaaaa actactgggc gaagccgtca ccaacattgg cgagcgacca aagcgctttg
     1801 ccgaagttat ccgtaaaacc aaagagaccg acatcaaagt gcaagtgtgg ctggacgaaa
     1861 caggcaacaa cgaaatcagc accggcgtgg gctttttcga ccatatgctt gaccaaattg
     1921 ccacccacgg cggttttaga atgaacatca ccaccaaagg cgacttgtgg attgacgaac
     1981 atcacacggt ggaagacacc gccctcgccc tcggcaccgc attaaaacaa gcgattggcg
     2041 acaaacgcgg catcgcccgt ttcggcttcg tgttgccgat ggacgaaagc cgtgcggaat
     2101 gtgcgatgga cttatcaggc agacctttca tcaagttcaa agcggatttc aaacgcgaca
     2161 aagtgggcga tttcagcacc gagctcaccg aacacttctt ccaatccatc gccttcacga
     2221 tgctcgccac cctgcacatc aaggcaaaag gcgacaacga ccaccacaaa atcgaaagcc
     2281 tgttcaaagt cttcggcaga acactcagac agtgcattcg tattgaaggg aatgaactac
     2341 caagcagtaa aggtgtatta taatcaccgt aggggcgggt tttaacccgc ccgaaagtgc
     2401 ggttgaaatt tgcaaagttt ttggggattt tgaccgctt
//
LOCUS       DANLXF010000276         2415 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.277, whole genome shotgun sequence.
ACCESSION   DANLXF010000276 DANLXF010000000
VERSION     DANLXF010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.277"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            16..930
                     /locus_tag="PXD66_002375"
     CDS             16..930
                     /locus_tag="PXD66_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HDL6320617.1"
                     /translation="MKLNYLYSPPQAVGRLKAEYADFIVREELGYELAGEGEFVAVKI
                     RKTNASTLFVGEKLAKFVGISEREMSYAGLKDRHNRKIRVGSLAGNHFELLLRDVAES
                     EELIYRLQQLQAVGFPNYFTEQRFGRDGHNLTQALRWANGEISVKDRKKRSFYLSAAR
                     SEVFNLVVSQRINDCLTQKILENDYVQLAGSNSFFCVKSDEIAETQARLAQQDLLLTA
                     PLIGERSLELECNPAEKAIIEQHAQLVALMQKERMAAARRAMLCKPQNLEWQFEQEGL
                     RLKFFLDSGSYATALVRELILLTEESEA"
     gene            1101..1889
                     /gene="surE"
                     /locus_tag="PXD66_002376"
     CDS             1101..1889
                     /gene="surE"
                     /locus_tag="PXD66_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HDL6320618.1"
                     /translation="MRILISNDDGFHAVGIQTLAKALRDAGHIVTIIAPDRNRSAASS
                     CLTLVDPLRVHRFDEYNYAVIAGTPADCVHLALNGLFDGKFAEKFDLVVSGINHGANL
                     GDDVVYSGTVAAALEGRHLPLPSIAVSLVGRKHSSFLDGENHFGTAAQVVLDLLEKME
                     TQRFPSNHVLNVNVPNLPYSELKGTMITRLGERSPAAEIVKQKDPRNADVYWIGVTGE
                     PLDESFGTDFYALNRDCVSITPIQVDMTAHKTIATLKGIFNETV"
     gene            1876..>2415
                     /locus_tag="PXD66_002377"
     CDS             1876..>2415
                     /locus_tag="PXD66_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDL6320619.1"
                     /translation="MKLFETIYDKTMQWSKHRFAAFWLSFVSFIEAIFFPIPPDVMLI
                     PMSMAKPQNAFKYAVYTTLASVAGGVIGYFIGLYATDWVQGVIANWGMQANFDKAKLW
                     FETWGVAVVFLAGFSPIPYKVFTICAGVMQMAFFPFVITAAISRFARFILVAKLSAWG
                     GKKYEQKIRRSIELIGWGTI"
BASE COUNT          672 a          484 c          557 g          702 t
ORIGIN      
        1 ttattcgaga agtgaatgaa actgaattat ttatactctc ctcctcaagc tgttgggcga
       61 ctaaaagccg aatatgcaga ctttattgtg cgtgaagaac tgggctacga actagccggc
      121 gaaggggaat ttgtggcggt taaaatccgc aaaaccaacg ccagcacgct gtttgtgggg
      181 gaaaagttag ccaaatttgt cggtatttcc gagcgtgaaa tgagttatgc aggcttaaag
      241 gaccgccaca accgcaaaat tcgtgttggt tcgctcgccg gcaatcattt tgaattattg
      301 ctacgtgatg tagcagaaag cgaagagttg atttaccgtt tgcaacagtt gcaagcggtc
      361 ggatttccca attattttac cgaacaacgt ttcgggcgag acggacataa cctgactcaa
      421 gccttgcgtt gggcaaacgg ggaaattagc gtgaaagatc gcaaaaaacg cagtttctac
      481 ctttccgctg cccgctcgga agtgtttaat ttagtggttt cacagcggat taatgactgc
      541 ttaacccaaa aaatcttgga gaatgattac gttcaacttg ccggctctaa cagctttttc
      601 tgcgtgaaat cggacgaaat tgctgaaact caagcccgtt tagcacagca agatctactt
      661 ctcaccgctc cgctgatagg cgaaagatcg cttgaattag aatgcaatcc tgctgaaaaa
      721 gccatcatag agcaacacgc tcaattggtt gccttaatgc aaaaagagcg tatggcagcc
      781 gctcggcgag caatgctttg taagccacaa aatctggaat ggcaatttga acaagaaggg
      841 ctacggttaa aattcttttt agattcaggc agttatgcca ctgcactggt gcgagagtta
      901 attctgctta ccgaagagag cgaagcttag tccataacac ataacaagcg gtaagatttt
      961 aatcattttt tgcaatttgc aaaactttaa caaaatctta ccgctttttg cacttttaga
     1021 ctaaaaaaac tgtcgaaaaa ttgttagaat tgcgatcttt gtatttagag tctatcaaga
     1081 tgtaagatct aaaggaaatt atgcgaattt taattagtaa tgacgatggt tttcacgctg
     1141 tcggcattca aactttagcc aaagccttgc gagatgcagg ccatatcgtc accattattg
     1201 caccggatcg taaccgcagt gcagcttcaa gctgcctaac cttagtagat ccacttcggg
     1261 tgcatcgttt tgatgaatat aattatgcgg tgatcgccgg cacacctgcc gattgcgtgc
     1321 atttagcctt aaacggcttg tttgacggca aatttgcaga aaaatttgat ttggtggtct
     1381 ccggcattaa tcacggggca aatttaggcg atgatgtggt ttactccggt acggtagcgg
     1441 cagcattgga aggccgccac ttgccattac caagtattgc agtatcactg gttgggcgta
     1501 aacactcttc gtttttagac ggtgaaaatc attttggaac ggcagcacag gtagtgttag
     1561 atttattgga aaaaatggaa acgcaacgtt ttccatcaaa ccacgttctc aatgttaatg
     1621 tgccgaattt accttattca gagctaaaag gcacaatgat cacccgctta ggtgaacgtt
     1681 cacccgctgc ggaaattgtg aagcaaaaag atcctcgtaa tgctgatgtc tattggattg
     1741 gtgtaacagg tgaaccactg gatgaaagtt ttggtactga tttttatgca ttaaaccgag
     1801 attgcgtttc tatcacacca attcaagtgg atatgaccgc tcataaaacg atagcaacct
     1861 taaaaggcat ttttaatgaa actgtttgaa acaatttatg acaaaacaat gcagtggtct
     1921 aagcaccgct tcgctgcctt ttggttgagt tttgtcagtt ttatcgaggc gatctttttc
     1981 ccgattccgc cggatgtgat gttgattccg atgtcgatgg caaaaccgca aaatgcgttt
     2041 aaatatgcag tttacactac ccttgcttcg gttgccggtg gtgttattgg ttattttatt
     2101 ggcttatatg ctaccgattg ggtgcaaggc gtcatcgcaa attggggaat gcaagcaaac
     2161 tttgataaag caaaattgtg gtttgaaact tggggtgtcg cggtagtatt tttagccggt
     2221 ttttcgccga ttccctataa ggtatttacc atctgtgccg gtgtaatgca gatggcgttc
     2281 ttcccgtttg tgattactgc tgcgatttct cgctttgccc gttttatttt ggtagcaaaa
     2341 ctctctgctt ggggtggcaa aaaatatgaa caaaaaattc gccgctcaat tgaattaatt
     2401 ggttggggaa caatc
//
LOCUS       DANLXF010000277         2405 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.278, whole genome shotgun sequence.
ACCESSION   DANLXF010000277 DANLXF010000000
VERSION     DANLXF010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.278"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..252
                     /locus_tag="PXD66_002378"
     CDS             <1..252
                     /locus_tag="PXD66_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320620.1"
                     /translation="SYSGETTASPLLKLDTERNINLYFQAIHKCTPDKIHTKIINIPS
                     EGNAKELWTATGCNKTQSFIITYRNDGVGGTFINMTTTP"
     gene            269..1840
                     /gene="guaA"
                     /locus_tag="PXD66_002379"
     CDS             269..1840
                     /gene="guaA"
                     /locus_tag="PXD66_002379"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012510808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDL6320621.1"
                     /translation="MTNIHHHKILILDFGSQYTQLIARRVREIGVYCELWAWDVTEEQ
                     IREFNPTGIILSGGPESTTEENSPRAPEYVFNAGLPVLGICYGMQTMAMQLGGLTETS
                     DHREFGYASVALKSADQLFANLNDDLTACEPKLDVWMSHGDKVTKLPPHFTITGVTPT
                     CPIAAMSDESRRFYGVQFHPEVTHTKQGLALLKNFVVGICGCETNWTAENIIEDAVKR
                     IREQVGDDEVILGLSGGVDSSVTALLLHRAIGKNLHCVFVDNGLLRLNEGDQVMEMFG
                     NKFGLNIVRVNAEDRFLEALKGLDEPEAKRKTIGKVFVDVFDDESKKLSSVKWLAQGT
                     IYPDVIESAASKTGKAHVIKSHHNVGGLPDYMKLGLVEPLRELFKDEVRKIGLALGLP
                     AEMLNRHPFPGPGLGVRVLGEIKKEYCDLLRKADAIFIEELHKAGWYYKVSQAFTVFL
                     PVKSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDLLGKISNRIINEVNGISRV
                     VYDVSGKPPATIEWE"
     gene            complement(2001..2396)
                     /locus_tag="PXD66_002380"
     CDS             complement(2001..2396)
                     /locus_tag="PXD66_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcB family protein"
                     /protein_id="HDL6320622.1"
                     /translation="MEQWTTDVWAAIMAAFIVGAVIGCTLVRVFNSSVRKQHELEKEL
                     KQTKTQVDEQKQKLEQHFEQSAQLLSSLASDYKKLYTHLAQSSEKLLPEASKNITFFQ
                     QTKLPIPAEKTEEDQPRDYSEGSSGLLKS"
BASE COUNT          681 a          517 c          558 g          649 t
ORIGIN      
        1 tcttattcag gagaaacaac ggccagtcct ttattaaagt tggacacaga aagaaatata
       61 aatctgtatt ttcaagcaat acataaatgt actcctgata aaattcatac aaaaatcatc
      121 aatattccaa gtgaaggtaa tgcaaaagaa ctttggactg caacaggttg taataaaacg
      181 caatctttta ttattacata tagaaacgat ggggtaggcg gcacttttat taacatgacc
      241 acaacgccat aatttaaagg aaattacaat gacaaacatc caccaccaca aaattttaat
      301 tttagacttc ggttcacaat atactcagct tatcgcacgc cgtgtgcggg agattggggt
      361 ttactgcgag ctttgggctt gggacgtaac ggaagagcag atccgtgaat ttaacccaac
      421 ggggattatt ctttcaggag gtcctgaaag caccactgag gaaaacagcc cacgagcgcc
      481 ggagtatgtg tttaacgctg gtctgccagt gctgggcatt tgctacggaa tgcagacaat
      541 ggcgatgcag ttgggcggtt taaccgaaac gtccgaccac cgtgaatttg gctatgcctc
      601 ggtggctctc aaatctgccg atcaactttt tgcaaattta aacgatgatt tgaccgcttg
      661 cgagccaaaa ttggacgtgt ggatgagtca cggcgacaaa gtcaccaaat taccaccgca
      721 ctttaccatc acaggcgtta ccccaacctg cccgattgcc gcgatgtcgg acgaaagccg
      781 ccgtttctat ggcgtgcagt tccaccccga agtgacccac accaaacaag gcttggcgtt
      841 gctgaaaaac ttcgtggtcg gcatttgcgg ctgtgaaacg aactggacgg cagaaaacat
      901 cattgaagat gcggtgaaac gcattcgtga gcaagtgggc gatgacgagg tgattttagg
      961 tttgtcgggc ggggtggatt cctccgtcac cgccttgcta ttacatcgtg ctatcggtaa
     1021 aaacctgcac tgcgtatttg tggataacgg cttgctccgc ttgaacgaag gcgatcaagt
     1081 catggaaatg ttcggcaaca aattcggttt gaacattgtg cgagtgaatg ccgaagaccg
     1141 tttcttagaa gcgttaaaag gcttagacga accggaagct aaacgcaaaa ccattggtaa
     1201 agtgtttgtg gacgtgtttg atgacgaaag caaaaaactt agctcggtca aatggcttgc
     1261 acaaggcacg atctaccctg atgtgattga atccgccgcc agcaaaaccg gcaaagccca
     1321 cgtgattaaa tcacaccata acgttggcgg cttgccggat tatatgaagc tcggcttagt
     1381 cgaaccgttg cgtgaattgt ttaaagacga agtgcgcaaa atcggcttgg cactcggctt
     1441 accagctgaa atgctcaacc gccacccgtt ccccggcccg ggcttaggcg tgcgtgtgct
     1501 gggcgaaatc aaaaaagagt actgcgattt actgcgtaaa gccgatgcga tcttcatcga
     1561 agaactgcac aaagccggct ggtactacaa agtcagccaa gctttcacgg tattcttgcc
     1621 ggtgaaatcg gtgggcgtaa tgggcgatgg acgcaaatat gactgggtcg tcagcctgcg
     1681 tgcggtggaa accatcgact ttatgaccgc tcactgggcg cacttgccgt atgatctatt
     1741 aggcaaaatc tccaaccgca ttattaacga agtgaacggt atttcccgtg tggtgtatga
     1801 cgtgagcgga aaaccaccgg caaccattga gtgggaataa ctcttacaaa ataggcttct
     1861 tataacctaa gttcagtatc taaagctgaa aaatgttgtt aactgcttaa tgtagcggtt
     1921 tagaataaag gattgattcc aacggaatca atccttaaac taagtgcctt ttagtaaaac
     1981 taaatattca atttttagtt ttatgatttc agtaagccag atgaaccttc ggaataatct
     2041 ctaggttgat cttcttccgt tttttctgcc ggaataggta atttggtttg ttggaaaaag
     2101 gtaatatttt ttgatgcttc tggtaatagc ttttcagagc tttgtgctaa atgggtataa
     2161 agctttttat aatcagaagc tagacttgaa agtaattggg ctgattgctc aaagtgttgt
     2221 tcaagttttt gtttttgctc atccacttga gttttggttt gcttcaactc tttttccagt
     2281 tcatgctgtt ttctgacact actgttaaat actctaacta gagtgcaacc tattacagca
     2341 cctacaataa atgctgccat aatggctgcc caaacatctg ttgtccattg ttccatcata
     2401 aactc
//
LOCUS       DANLXF010000278         2399 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.279, whole genome shotgun sequence.
ACCESSION   DANLXF010000278 DANLXF010000000
VERSION     DANLXF010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.279"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..334
                     /locus_tag="PXD66_002381"
     CDS             <1..334
                     /locus_tag="PXD66_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL6320623.1"
                     /translation="DELVTINEKIVNLTYGDQDALNIYFKGGWGALPIEYNYQVDAIL
                     ELVLRREQEELARKNGYLDVIPKIIHYTSKFKPWKKELHTMQISTRKKYWFYYHLEWN
                     DILEQHQK"
     gene            complement(380..667)
                     /locus_tag="PXD66_002382"
     CDS             complement(380..667)
                     /locus_tag="PXD66_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HDL6320624.1"
                     /translation="MKNDKPIECEGCNTFDVGSILDNSETVATIQRTYESENEAKAAL
                     EKFTQKARDVESEPCEISSEITPIESGFELVAHFKFSCQAEVVIFQLGTRF"
     gene            787..1200
                     /locus_tag="PXD66_002383"
     CDS             787..1200
                     /locus_tag="PXD66_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PACE efflux transporter"
                     /protein_id="HDL6320625.1"
                     /translation="MTAWERVFHALLFECFAIIFTVILTSWLTSHRMVDLTAVIVMIS
                     VIAMVWNVIFNWGFDKVFTGERVQRGLGIRILHSFLFEGGLLVFTIPLVMYMLDIGWW
                     QAFVMDIGMTLFVLVYSVVFNWVYDHLRLRWVKSG"
     gene            1285..2277
                     /gene="mglB"
                     /locus_tag="PXD66_002384"
     CDS             1285..2277
                     /gene="mglB"
                     /locus_tag="PXD66_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HDL6320626.1"
                     /translation="MKRTILSAFALSVICGVNLSYAQSESRVGVTIYKYDDHFMTQMR
                     KDMQNEAKGFAGLKWFMNDAQNSQVKQLAQIETLLQHKMRVLAVNIVNANESKTIVEK
                     AKAYNVPVVFFNRTPSKQALESYEKAYFVSADPKEAGRIQGELIAKVWKSNPDFDLNK
                     DGKLQFVLLKGEPSDVSAERSQAVVESLNQQGVQAEEIYSDTARWRRTLARNKMNEWL
                     VENRAAEIEVVISNNDEMAIGALDALNAHEKRLPIFGIDALPETLALMKKGEIAGTVL
                     NDSSAQAKAVVKLVSNLAQGKPALKDTQWESHSSKTIYLPHIGIDKENLGQFLP"
BASE COUNT          714 a          378 c          556 g          751 t
ORIGIN      
        1 tgatgagttg gttactatca atgaaaaaat tgtaaactta acttatggcg atcaagatgc
       61 tctcaatatt tactttaaag gcggttgggg agcattgccg atagagtata attatcaagt
      121 tgatgcaatt cttgaactag tattaagaag agaacaagag gaacttgcga gaaaaaatgg
      181 ctatttagat gtgattccga aaattatcca ctatacgagt aaattcaaac cttggaaaaa
      241 agaactgcat acaatgcaaa tatctacgcg taaaaaatat tggttttact atcatttaga
      301 gtggaacgat attttggaac aacatcaaaa atagctatca tacaaaagct gattcaagtg
      361 tttgaatcag ctttttgtat tagaaacgtg ttcctaattg aaaaataacc acttctgctt
      421 ggcagctaaa tttgaagtga gcaaccagtt caaaaccgct ttcaatcggg gtgatttcac
      481 tgctgatttc acagggttca gattcgacat cacgggcttt ttgagtgaat ttttcaagtg
      541 ccgctttagc ttcattttcg ctttcataag tgcgctgaat agtagcgact gtttcgctgt
      601 tatctaaaat agaacctaca tcaaatgtgt tacagccttc gcattcaatc ggtttatcgt
      661 ttttcattgg ctaatccttc taaattgatg aataatgtag gtactttaaa actaaattgc
      721 gtaacctgca atcatcagtg aacgattatt tgatttagtt ggtattttgc aaagtttgga
      781 ggaaaaatga ccgcttggga gcgtgtgttt cacgcgctgt tatttgaatg tttcgctatt
      841 atttttacgg ttatcttaac ttcttggctg acttctcatc gtatggttga tttaaccgca
      901 gtgattgtga tgatttcggt gattgcaatg gtgtggaatg ttatttttaa ctggggtttt
      961 gataaggtgt ttaccggtga gcgagtgcaa cgtggtttag gcattcgtat tttgcattca
     1021 ttcctttttg agggcggttt gctggttttt actatcccat tggtaatgta tatgctcgat
     1081 atcggctggt ggcaggcgtt tgtgatggat attggaatga ccttatttgt attagtttat
     1141 tcggtagttt ttaattgggt gtacgatcat ttacgcctga ggtgggttaa atctggctaa
     1201 attgtgaact agatcacggt ttccttacac aaaattcgct atgcttgaat tggttcatat
     1261 ttggcatagc aagagggaca ttctatgaag cgtacaatct tgagtgcttt tgcactttct
     1321 gttatttgcg gtgtgaatct aagttacgca caatctgaaa gcagagtggg ggtaacgatt
     1381 tacaaatatg atgaccattt tatgactcaa atgcgtaagg atatgcaaaa cgaggcgaaa
     1441 ggcttcgctg ggttgaagtg gtttatgaat gatgcacaaa attcccaagt gaagcaatta
     1501 gcgcaaattg aaaccttact tcagcataaa atgcgtgtgt tggcggtgaa tattgtgaat
     1561 gcaaatgagt cgaaaactat tgtggaaaaa gcgaaggctt ataatgtgcc ggttgtcttt
     1621 tttaaccgca caccgagtaa acaagcgtta gaaagttacg aaaaggcgta ctttgtgagc
     1681 gctgatccta aagaggccgg tagaatccaa ggggaattaa ttgcgaaagt gtggaaatca
     1741 aaccccgatt tcgatctaaa taaagacggg aaattgcaat ttgtattgct taagggcgag
     1801 ccgagtgatg tgagcgctga gcgttctcaa gctgtggttg aatcgcttaa tcaacaaggt
     1861 gttcaggcag aagaaattta cagtgacacg gcgagatggc gtagaacatt ggctcgcaat
     1921 aaaatgaatg agtggctggt ggaaaaccgt gcagctgaga ttgaagttgt gatttcaaac
     1981 aatgatgaaa tggcaattgg tgctttagat gcgttaaatg cacacgagaa aagattgccg
     2041 atctttggta ttgatgcctt gcctgagacg ttagcattaa tgaaaaaagg ggaaatagcc
     2101 ggcacggtat taaatgatag ctctgcacaa gcgaaagcgg tggtgaaatt ggtatctaat
     2161 ttggcacaag gtaagcctgc gctgaaagat acgcagtggg aaagccattc ctctaaaacg
     2221 atttatcttc cacatattgg tattgataag gaaaatttag ggcaattttt accttaaatg
     2281 gaggagcggg gcgttataaa tacgcccctt attgatgata acaagcggtc atttttcagt
     2341 aaaatcttgc aaatcctttc tcttttcttc caacagattt gaatagagcg aatattcaa
//
LOCUS       DANLXF010000279         2367 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.280, whole genome shotgun sequence.
ACCESSION   DANLXF010000279 DANLXF010000000
VERSION     DANLXF010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.280"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..358
                     /locus_tag="PXD66_002385"
     CDS             <1..358
                     /locus_tag="PXD66_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDL6320627.1"
                     /translation="DKTALVSKNSTLGIGVFVGKMAIVNSGVTVGDNVIINTKSLVEH
                     GCFIGSHCNISTNTTLNGDVIVEDHAFIGSSSVVNGQLRVGESALVGSGAVVIRNVEP
                     RTVVAGVPAKYIKDIK"
     gene            358..1395
                     /gene="neuB"
                     /locus_tag="PXD66_002386"
     CDS             358..1395
                     /gene="neuB"
                     /locus_tag="PXD66_002386"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HDL6320628.1"
                     /translation="MVNVFIVAEIGCNHNGDPALAKKMVDVAKECGVDAVKFQTFKAD
                     KLISKYAPKAEYQKVTTGTADSQLEMTRKLELPYEEFVKLEAYARSLGLEVFSTPFDF
                     ESIDFLASQQQKVWKIPSGELTNLPYLEKIARLPIEGKTIVISTGMATIEETKASLKV
                     LEDNGVAKKDITILHCNTEYPTPYEDVNLNAFHQLKQEFGDYSLGFSDHSAGYFAGLA
                     AVPYGITFIEKHFTLDKNFEGPDHKASVTPEELKLLCEGIRAVEKSLGSSEKLVTNSE
                     AKNKIVARKSIIAARPIKKGEIFTTENITTKRPGNGISPMFWYDVLGKEAQQDFEEDQ
                     LIQDSRFENQM"
     gene            1455..>2367
                     /locus_tag="PXD66_002387"
     CDS             1455..>2367
                     /locus_tag="PXD66_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HDL6320629.1"
                     /translation="MKKIAIITARAGSKGLPNKNVLLANGKPLMAYSIEAAIESGQFE
                     KIIVSTDSQEYIDLFSHYPIEFIKRAEHLASDKASSFVVIEDVLNQYRSVEFDYFMLL
                     QPTSPLRSGEHIKESCEKFEKNFEQFDFLVSVSDAHKPTTLTRMIEEDECLKHFQLDY
                     SNYARQLHHPEYSPNGAIFTAKPEAYLKQKHFYGAKCLAYFMDKDVSIDIDDRQDFEY
                     FYFILQQRNKEKILLETIKRSILMKKENFKEVKEIALIGHSIIEYWDIEHLNGKAVNN
                     LGIAGISTKQYSDLILDEGLISALPKDI"
BASE COUNT          773 a          391 c          502 g          701 t
ORIGIN      
        1 agataaaacc gcattggtgt ccaaaaattc cactctaggc attggagtgt ttgttggaaa
       61 aatggcaatt gtaaacagtg gcgtaactgt aggcgataat gtgatcatta acactaaatc
      121 gctggttgag catggttgtt ttatcggcag tcattgcaat atttcaacca atacgacact
      181 aaatggcgat gtaattgtcg aagatcatgc ttttattggt agttcctctg ttgtgaacgg
      241 acagctaaga gtgggtgaaa gtgcattggt tggctccggt gcagtggtaa ttagaaatgt
      301 ggagcctcgc acagttgtag caggcgttcc agcaaaatat attaaggata taaaataatg
      361 gtaaatgtat ttattgtggc agaaatcggt tgtaaccata acggcgatcc tgcgttagca
      421 aagaaaatgg ttgatgtagc gaaagagtgt ggtgtcgatg cggtgaaatt ccaaaccttt
      481 aaagcggata agttgatctc taaatatgcc cctaaagccg agtatcaaaa agtaacgacc
      541 ggcaccgctg attcccaatt agaaatgacc cgcaaattag agttacctta tgaggagttt
      601 gtgaaattag aggcttacgc tcgttcattg gggttagagg tattctctac gccattcgat
      661 ttcgagtcga ttgatttctt ggcaagccaa caacaaaaag tgtggaaaat tccgtctggt
      721 gaattaacca atttgccgta tttagaaaaa attgcacgac ttccgattga aggcaagacg
      781 atcgttattt ctaccggtat ggcaaccatt gaagaaacaa aagcctcatt aaaagtattg
      841 gaagataacg gtgtagcgaa aaaagatatt acgattttac attgtaatac cgagtacccg
      901 acaccttatg aagatgtgaa tttaaatgcg ttccatcagc taaaacagga gtttggcgac
      961 tatagtttag gtttttctga ccactctgcc ggttattttg cagggttggc ggcagtacct
     1021 tacggcatta ccttcattga aaaacatttt accttagata aaaactttga aggtccggat
     1081 cataaagctt cggttacacc tgaagagctg aaattactgt gtgaaggcat tcgtgcggtg
     1141 gaaaaatcgc tgggttcttc cgagaaatta gtcactaatt cggaagcaaa aaataaaatt
     1201 gtggcaagaa aatcaattat tgcggcacgt ccaattaaaa aaggcgaaat ttttacaacc
     1261 gaaaatatta cgactaaacg ccccggtaat ggcattagcc cgatgttttg gtatgatgtg
     1321 ctaggtaaag aagcacaaca ggattttgaa gaagatcaat taattcaaga ttctcgtttt
     1381 gaaaatcaga tgtaatataa tatagtgatg ggatgaaagc tccatcattt tgtgtttaac
     1441 aagcggtcaa atttatgaaa aaaattgcaa ttattacagc ccgagccggt tcaaaaggct
     1501 tgccgaataa aaatgtatta ttagccaacg gtaagccgtt aatggcctat tctattgaag
     1561 ctgcgattga gtcggggcaa tttgagaaaa ttatcgttag tactgattcc caagaataca
     1621 ttgatttgtt ctcacattat ccaattgaat ttattaaacg tgccgagcat ttagccagtg
     1681 ataaggcgag ttcttttgtc gtgattgaag atgtgttaaa tcaatataga tcagtagagt
     1741 ttgactattt tatgctactt caaccaactt cgccgttgag atcaggagag catattaagg
     1801 aatcgtgtga aaaatttgag aaaaatttcg aacaatttga tttcttagta tctgtttctg
     1861 atgcccataa acctacgaca ttgactcgta tgattgagga agacgagtgc ctaaagcatt
     1921 ttcagttgga ttattctaac tatgcacgcc aattgcatca tcctgaatat tcaccgaatg
     1981 gagctatttt tacagctaag ccagaagcct atcttaaaca aaaacacttc tatggtgcga
     2041 aatgtctagc ctattttatg gataaagacg tttctattga tattgatgat cgccaagatt
     2101 tcgagtactt ctactttatt ctgcaacaac gcaataaaga aaaaatctta cttgaaacca
     2161 ttaaacgttc gattttaatg aaaaaagaga actttaaaga agtgaaagag atagccttaa
     2221 tcgggcattc gatcattgaa tattgggata ttgaacattt aaacggtaag gcagttaata
     2281 atttaggaat tgcaggtatt tcaacaaaac aatacagtga tctgattttg gatgaaggtt
     2341 taatttctgc tttacctaaa gacatta
//
LOCUS       DANLXF010000280         2328 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.281, whole genome shotgun sequence.
ACCESSION   DANLXF010000280 DANLXF010000000
VERSION     DANLXF010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.281"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(250..1383)
                     /locus_tag="PXD66_002388"
     CDS             complement(250..1383)
                     /locus_tag="PXD66_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis PLP-dependent protein"
                     /protein_id="HDL6320630.1"
                     /translation="MTKFNNIETTLVQIGNRTDPRTGAVAMPIILSTAYGRDGLGEST
                     GWDYTRTKNPTRAVLEQGIADLEGGDAGFAMASGMAAIQLVMSLFKAPDEWIISSDVY
                     GGSYRLFDFSHKNHGTLKPVYVNTADLAAIEAAITPNTKAIFVETPSNPLMEECDVAA
                     IAKIAKKHNLMLIVDNTFLTPALFRPIEHGADIVIHSATKYLSGHNDVLAGLIVAKDS
                     DATKVNGQNLTERLFYFQNCAGAVLSPFDSYLAVRGMKTLALRMERHQSNATELAKFL
                     AEQPEIDSVLYSGKSGMLSFRLQKEEWVAKFLKAIKLITFAESLGGAESFITYPATQT
                     HMDIPEAERITRGITNNLLRFSVGLEHVEDIKADLLQAFKQLK"
     gene            complement(1400..2212)
                     /gene="dapB"
                     /locus_tag="PXD66_002389"
     CDS             complement(1400..2212)
                     /gene="dapB"
                     /locus_tag="PXD66_002389"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005696006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDL6320631.1"
                     /translation="MTLKIGIVGAGGRMGRQLIQAVHNAQGVELGSAFERKGSSLVGS
                     DAGEVAGVGTLGVVISDDVAQNVAGFDLLIDFTRPEGTLEHLDICVAHHKQMVIGTTG
                     FDDAGKAAIKAASEKIGIVFASNYSVGVNLVFKLLEKAAKVMGDYCDIEVIEAHHRHK
                     VDAPSGTALSMGEHIAKTLGRDLKTHGVFERNGITGERKRDEIGFATIRAGDVVGEHS
                     VWFADEGERVEIAHKASSRMTFANGAVRAAKWLNGKQNGLFDMTDVLDLNNL"
BASE COUNT          683 a          533 c          407 g          705 t
ORIGIN      
        1 ttgaacaaac gaagcgacag cgttagcaaa aacttaaaat gatgtacgga tttaaaaatc
       61 tgttcgtttg cttgttttat aagcaaacta atttcgcata attattatta tgttaaatag
      121 atagttaaaa taacacattt aattaaatct tttcgtgttt ctctcctcct ttattaaaaa
      181 agaaaacggt cagattggta aaatctatac ctaatatgac cgcttgtgaa tacaaaaaaa
      241 ttacttcaat tacttcaact gtttaaaggc ttgcagtaaa tcagctttaa tatcttcaac
      301 gtgttctaaa ccaacggaaa aacgcagtaa attattcgta atgcctcgtg taatacgttc
      361 tgcttccgga atatccatat gggtttgtgt tgcaggataa gtaataaaac tttctgcacc
      421 gcctaaactt tccgcaaagg taatcaattt aattgccttg agaaatttcg ctacccactc
      481 ttctttttgt aaacggaaag ataacatacc acttttgcct gaatataaca cgctgtcaat
      541 ttcaggttgc tctgctaaga atttagccag ttcggtggca ttagactgat ggcgttccat
      601 tcgtaatgcc aaggttttca taccacgcac ggcaaggtaa gaatcaaatg gcgacagcac
      661 tgcaccggca cagttttgga aatagaataa acgctcggtc aaattctgcc cgtttacttt
      721 ggtagcatca gaatctttcg caacaattaa gccggctaac acatcattat gccccgaaag
      781 atacttggtc gcactgtgaa tcacaatatc agctccgtgc tcaatcggac ggaataacgc
      841 tggggttaag aacgtattat caacgatcaa cattaaattg tgttttttcg caattttagc
      901 aatggcagca acgtcgcact cctccatcaa tggatttgac ggcgtttcca cgaaaatcgc
      961 cttagtattc ggtgtaatag ctgcttcaat cgctgctaaa tccgctgtat taacataaac
     1021 cggctttaat gtgccgtgat ttttgtggct aaaatcaaac aagcggtaag aaccaccata
     1081 aacatcgctg gaaataatcc attcatcagg tgctttaaat aatgacatta ctagctgaat
     1141 cgctgccatt ccagatgcca tcgcaaaacc ggcatcaccg ccttctaaat ccgcaatccc
     1201 ctgctctaat acagctcgag ttgggttttt ggtacgggtg taatcccagc cggtagattc
     1261 gcctaagcca tcacgcccgt aagcagttga taagataatc ggcattgcca ccgcccctgt
     1321 acgaggatca gttctgttac cgatttgcac taatgtggtt tcaatattat tgaattttgt
     1381 cattgttatt atccttcaat tacagattat ttagatccaa tacgtctgtc atatcaaaca
     1441 agccattttg tttgccattt aaccattttg cagcacgcac tgcgccattg gcaaacgtca
     1501 ttcgactaga ggctttatgt gcgatttcga cacgttcgcc ttcatcagcg aaccatacac
     1561 tatgctcccc tacaacatca ccggcacgaa tagtagcaaa gccgatttca tcacgtttgc
     1621 gttcgcctgt aataccgtta cgttcaaata cgccatgagt tttcaagtca cgcccaagag
     1681 ttttggcaat atgttctccc attgataaag ccgtacctga tggtgcatca actttatggc
     1741 gatggtgtgc ctcaatcact tcaatatcac aataatcgcc cattactttt gctgcttttt
     1801 cgagcagttt aaacaccaga ttcaccccaa cgctgtagtt tgaggcaaaa acgataccga
     1861 ttttttcact cgctgctttg attgcagctt tacctgcatc atcaaaaccg gttgtgccaa
     1921 tcaccatttg tttatgatga gccacacaaa tatccaaatg ttctagcgtg ccttcagggc
     1981 gagtaaaatc aattagtaaa tcaaaacctg ctacattttg ggcgacatca tcagaaatca
     2041 ccacacctaa tgtaccaacc ccggctactt caccagcatc actacctact aatgatgaac
     2101 ctttacgctc aaaagcagac cctaattcta cgccttgtgc attatggacg gcttgaatta
     2161 attgacgccc cattcgccca ccggcaccga cgattccaat ttttaatgtc atatttgttc
     2221 ttccttttta tatttgtttt ttacaagcgg ttacattttt aattaatttt gcaaatagct
     2281 ttctgatggc aatttaaaag gtgggtgaga agtaaaatca acaatttg
//
LOCUS       DANLXF010000281         2320 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.282, whole genome shotgun sequence.
ACCESSION   DANLXF010000281 DANLXF010000000
VERSION     DANLXF010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.282"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            49..>2320
                     /gene="cas3"
                     /locus_tag="PXD66_002390"
     CDS             49..>2320
                     /gene="cas3"
                     /locus_tag="PXD66_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HDL6320632.1"
                     /translation="MEEHTIQSFYAHSGKLSDHSDWQTLLSHANNVGLLAAEFAAPFA
                     SQEIARCTGLLHDLGKYSERYQKRLHGGERVNHSTAGAKIAVERWGPLGKMMAFCIAG
                     HHAGLANGSGEGKNRSTLTQRLQEQFGYGHKDLPVLDAIWQQELNLPEKLPLPPLKPS
                     KNEPFFSYAFFIRMLYSCLVDADFLDTEAFYANLEKRPLERGNYPNLTALHQQFNQFI
                     AQFRERIATQTPNTQEAQRKAEVNRLRSEILDYAVSQAKQETGLFSLTVPTGGGKTFT
                     SMAFALEHAKQYGLCRIIYVIPFTSIIEQNAAEFRKAFGELGTEAVLEHHSTFDHDSL
                     PDKSSRDKLKLASENWDTPVVVTTAVQFFESLFADRSSRCRKLHNIVGSVIILDEAQM
                     LPLNLLRPIMAAIDELARNYQCSVVLCTATQPAIHQANGFYKGFENVREIAPNPTALF
                     EKLKRTTVKHIGIQTDVELQSKLADNPQMLIIVNNRRHARALYDSAKQLEGAYHLTTL
                     MCAKHRSQTLTEIRLRLKQNLPCRVIATSLIEAGVDVDFPLVMRAEAGLDSVAQAAGR
                     CNREGKRLSVESNVWVFQPEEQWKAPPELGLLSGCMRSTVQVHADDLLNPKAINHYFQ
                     AVYEAKGKELDKPNILKACHDAGQSLNFPFQKIAEDFRMITSHLIPVIIPFDQEAENL
                     IDRLRYADQVGGILRKLQPYTVQVPEKALAELYNAGRVETINETNFGKQFYCLIGMDL
                     YDEVAGLSWENVDFLES"
BASE COUNT          677 a          487 c          518 g          638 t
ORIGIN      
        1 tagaggtagt attgcttgtg ttaggaagga aatattgaag gtataaaaat ggaagaacat
       61 actattcagt ctttttatgc ccattcgggg aagctttcag atcattcaga ttggcaaact
      121 ttactcagcc acgccaataa tgttggcttg cttgccgccg aatttgctgc gccgtttgct
      181 tcacaagaaa ttgctcgctg cacggggcta ttgcacgatt taggcaaata tagtgaacgt
      241 taccaaaagc gtttacatgg cggagaacgg gtaaatcact ctactgctgg tgcaaaaatt
      301 gcagtggaac gttgggggcc tttgggcaaa atgatggcgt tttgtatcgc aggccaccac
      361 gctgggttag caaatggttc tggagaaggg aaaaatcgtt ctacacttac acaacgtttg
      421 caagagcaat ttggctatgg tcataaagat ctaccggtac ttgacgctat ttggcagcaa
      481 gaacttaatt tgcctgaaaa attgccctta ccgccgttaa agccttctaa aaacgaacct
      541 ttcttttctt acgccttttt tatccgaatg ctctattctt gccttgtcga tgccgacttt
      601 cttgatacag aagcttttta tgccaatctc gaaaaaagac cgcttgaacg aggaaattac
      661 cccaatttaa ccgcactcca tcagcaattt aatcaattta ttgcacagtt tagggagcga
      721 attgccactc aaacgcctaa tacgcaggaa gcacaacgta aagcggaagt aaaccgcttg
      781 cggagtgaaa ttttggatta tgccgtatct caagcaaaac aggaaacagg tctgttttct
      841 ctgactgtgc caacgggcgg tggtaaaacc tttacttcaa tggcgtttgc ccttgagcat
      901 gctaaacagt atggtttatg ccgcattatt tatgttattc cgtttaccag cattattgaa
      961 caaaatgctg cggaatttcg taaagctttt ggcgaattag gaacagaggc ggtattggaa
     1021 catcacagca cttttgatca cgatagccta cctgataaat caagccgaga taagctgaaa
     1081 ttggcatcag aaaattggga tactcctgtt gtggttacaa ctgcagtgca attctttgaa
     1141 agtttgtttg ccgaccgttc ttcacgctgt cgcaaattgc ataatatcgt cggtagcgtg
     1201 attattttgg acgaagccca aatgctaccg ttgaatttat tacgcccaat tatggctgcg
     1261 attgatgaat tagctcgcaa ttatcaatgt tcggtagtgc tttgcacagc gacacaaccc
     1321 gctattcatc aagcgaacgg tttttataaa ggatttgaaa atgtgcgaga aattgcccct
     1381 aatccaaccg cactttttga aaaattgaaa cgtactacgg taaagcatat cggtattcaa
     1441 acagatgtgg agttgcaaag caaactcgca gataacccac aaatgctgat aatcgtgaat
     1501 aaccgccgtc acgcccgagc gttatatgat tcagcgaaac agttagaggg agcttatcac
     1561 ctcaccacgc taatgtgtgc taaacaccgc tcacaaacgc tgactgaaat tcgtcttcgc
     1621 ctaaaacaaa acctaccttg ccgagtgatt gccacttctt taattgaagc cggtgttgat
     1681 gtcgatttcc cactggttat gcgagctgaa gcaggactag attcagtggc tcaagcagca
     1741 gggcgttgca accgagaggg gaaacgttta tctgtagaaa gcaatgtttg ggttttccaa
     1801 cctgaagaac agtggaaagc accaccagag ctaggcttac tttctggctg tatgcgttca
     1861 acggttcagg ttcacgcaga tgatctactt aatccgaaag caatcaatca ttatttccaa
     1921 gcggtttatg aggctaaagg aaaagagctg gataagccaa atattttaaa ggcgtgtcac
     1981 gacgctgggc aaagccttaa tttcccattc caaaaaatag ctgaagattt ccgaatgatc
     2041 acaagccatt taattccggt gattatccct ttcgaccaag aagccgaaaa ccttattgac
     2101 cgtttacgct atgcggatca agtaggcggt attttaagaa aactccagcc ttacactgtg
     2161 caagtaccgg aaaaagcatt agccgaactc tataacgctg gcagagttga aaccattaat
     2221 gaaaccaact tcggcaaaca gttttactgc ttaattggaa tggatttgta tgatgaagtg
     2281 gcggggttga gttgggagaa tgtggatttt cttgagtcgg
//
LOCUS       DANLXF010000282         2276 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.283, whole genome shotgun sequence.
ACCESSION   DANLXF010000282 DANLXF010000000
VERSION     DANLXF010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.283"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            74..532
                     /gene="mraZ"
                     /locus_tag="PXD66_002391"
     CDS             74..532
                     /gene="mraZ"
                     /locus_tag="PXD66_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDL6320633.1"
                     /translation="MFRGASSISIDSKGRIAIPTRYRAELLEKHHGILVCTVDIRQPC
                     LLLYPLHEWEMVEQKLLALSNFDPVQRRIQRVMQGFATECEMDSAGRILLSPTLRQHA
                     QLEQQIMLVGQLNKFEIWQDKQWQAQIAEDLAFGSSAEILDCEALKNLSL"
     gene            548..1486
                     /gene="rsmH"
                     /locus_tag="PXD66_002392"
     CDS             548..1486
                     /gene="rsmH"
                     /locus_tag="PXD66_002392"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDL6320634.1"
                     /translation="MSENPKHITVLLHEAVDGLAIKPNGIYIDGTFGRGGHSRLILSK
                     LGENGRLIATDRDPRAIAEAQTISDPRFQIEHTAFSAIPELCERMGLVGKIDGILLDL
                     GVSSPQLDEAERGFSFMRDGPLDMRMDTTKGLSAAEWLAQVSAEDLAWVLKEFGEERF
                     AKRIAQAVVSYNKSANEKISRTLQLAQIIADAVPFKDKHKHPATRSFQAIRIFINGEL
                     DELEKALNSALTVLAPEGRLSIISFHSLEDRMVKQFVRKHSKGMDVPRGLPILEAELT
                     KNIPLKTIGKAIMPSEAEIEANARSRSAVLRIAEKR"
     gene            1505..1816
                     /gene="ftsL"
                     /locus_tag="PXD66_002393"
     CDS             1505..1816
                     /gene="ftsL"
                     /locus_tag="PXD66_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDL6320635.1"
                     /translation="MSNERYPLHQIIIDDIFSHNKVALLLLIGVVISAVATIWITHQT
                     RLSVSEQGQLIQANQKLESQYTNLQLEENSRSSRARVDAAAKSFGLQPIKKEQEIILV
                     E"
     gene            1820..>2276
                     /locus_tag="PXD66_002394"
     CDS             1820..>2276
                     /locus_tag="PXD66_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsI"
                     /protein_id="HDL6320636.1"
                     /translation="MVKSVKLKRKQEAPVAKAEPQQAEGKKTPSFLPTRFRVVVFFML
                     LMVGGLLAQTAYIQLFDSERLIKEANNRSLRTKELQFTRGRILDRNGRFLSISVPMYS
                     LTLDPREYFDTKLRRSNESWRALAIEMETSKGKIEANVNKFIEKKNFSKE"
BASE COUNT          706 a          443 c          515 g          612 t
ORIGIN      
        1 taagtgggaa aaagtggtaa attgtggaac ttttccttaa atttaacctc atttaaaaag
       61 gtaaatcagt aaaatgtttc gtggtgcaag ttcaatcagc attgatagca aagggcggat
      121 cgctattccg acccgttatc gtgccgagct attggaaaaa catcacggga tcttagtttg
      181 cactgtggat atccgccagc cttgtttgtt gctttaccca cttcacgaat gggaaatggt
      241 tgaacagaaa ttgttggcac tatctaattt tgaccctgtt caacgccgta ttcagcgagt
      301 aatgcaaggc tttgctaccg aatgtgaaat ggattcggca gggcgaatac tgcttagccc
      361 tacacttcgc caacacgcac aattagagca acaaattatg ctggttggac aattaaacaa
      421 atttgaaatt tggcaagata aacaatggca agcacaaatt gccgaagatc ttgcttttgg
      481 cagttcggca gaaattttag actgcgaagc attgaaaaac ctgtctctct agtaattgga
      541 aattgagatg agcgagaacc caaaacatat tacagtatta ttgcacgaag cggttgacgg
      601 cttggcaatt aagcctaacg gtatctatat tgacggtact ttcgggcgtg gcgggcattc
      661 tcgcttgatt ttatccaaac tgggcgaaaa tgggcgatta atcgcaactg atcgtgaccc
      721 acgagcgata gccgaagctc aaacgattag tgaccctcgt tttcaaattg aacatacggc
      781 attttctgcc attcccgaac tgtgtgaacg aatggggttg gtgggcaaaa ttgacggtat
      841 tttattggat ttaggtgtgt cttccccgca gcttgacgaa gccgaacgag gctttagttt
      901 tatgcgtgac gggccgcttg atatgcgaat ggataccacc aaaggcttat cggcagccga
      961 gtggcttgcc caagtttcgg cagaagattt agcttgggta ttgaaagaat ttggcgaaga
     1021 gcgttttgcc aaacggattg cacaagcggt cgtttcttac aataaatctg caaatgaaaa
     1081 aatcagccgc actttgcagc ttgcgcaaat tattgcggat gcggtgccgt ttaaagataa
     1141 gcacaaacac cctgcaaccc gttcgttcca agcaattcgc atttttatca atggtgaatt
     1201 agacgagctg gaaaaagctt taaattccgc attaacggtg ttagcccctg aagggcgttt
     1261 gtcgattatc agctttcatt ctctagaaga ccgaatggtg aaacagtttg tgcgtaagca
     1321 tagtaaagga atggacgtac cccgtggttt accgatttta gaagcagagc taaccaaaaa
     1381 tattccgctg aaaaccattg gcaaagcgat tatgccgagc gaagccgaaa ttgaagcaaa
     1441 tgcccgctca cgcagtgcgg tattgcggat tgccgaaaaa cgctaacagt aaatagggaa
     1501 gaatatgtcg aacgaacgtt atccgttaca tcaaatcatc attgatgata tctttagcca
     1561 taacaaagtg gctcttttat tgttgattgg tgtagtgatt tcagcagtgg ctacgatttg
     1621 gataacccac cagactcgtt tatcggtgag cgaacaaggg caactgattc aagcaaacca
     1681 aaagttggaa agccaatata ctaatctgca attagaagaa aacagcagaa gcagtcgggc
     1741 aagagttgat gccgccgcta aatcgttcgg attacagccg attaaaaaag agcaagaaat
     1801 tattttagtt gaataaaaaa tggttaaatc agttaaatta aaacgtaagc aagaagcccc
     1861 tgtcgcaaaa gcagagcctc aacaagcaga aggtaaaaaa acaccgagtt ttttacctac
     1921 tcgctttaga gtggttgtgt tttttatgct attaatggtt ggtggcttac tagcacaaac
     1981 cgcctatatt cagctttttg actctgaaag attaattaaa gaggctaata accgctcact
     2041 tcgtactaaa gaactccaat ttacccgtgg acgtatttta gatcgtaacg ggcgttttct
     2101 ctctattagt gtgccaatgt actcattaac acttgacccg agagaatact ttgatactaa
     2161 gctgcgtcgt tctaacgaaa gctggcgtgc cttagcgatt gaaatggaaa catcaaaagg
     2221 taaaattgag gcaaacgtta ataaatttat tgaaaagaaa aatttttcaa aagaaa
//
LOCUS       DANLXF010000283         2275 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.284, whole genome shotgun sequence.
ACCESSION   DANLXF010000283 DANLXF010000000
VERSION     DANLXF010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.284"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..1827
                     /locus_tag="PXD66_002395"
     CDS             <1..1827
                     /locus_tag="PXD66_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HDL6320637.1"
                     /translation="TPEEAVRQLYLMNLHYEYDYPFERMELEYSVVFGREKKRADIVI
                     FDRDNPNAVYIMVELKKPKLKDGKEQLKSYCNATGAPIGVWTNGESISYYNRRDPNYF
                     KDIPDIPNAQQKLSDLLQERWTIQDLIDNDKIAKQRKSLKDLVIEMEDEVLANAGVDV
                     FEELFQLIFTKLYDEMESTRNKSRYLEFRNYGDTESELKAKIQSLFDRAKNKWEGVFA
                     ESAKINLSPSHLAICVSSLEEVKLFNSNLDVVDEAFEYLINKSSKGEKGQYFTPRYVI
                     DMCVKMLDPKPEETVIDTAAGSCGFPVHSIFHVWEKQLKARGLERSHLFTAEEKLPEQ
                     TDYVKEKVFAIDFDEKAVRVARTLNLIAGDGQTNVLHLNTLDYERWEDFTKEEEWNDV
                     YGEGWKKLRKLRKTKNENRDFQFDVLMANPPFAGDIKETRILARYELGKNSKGKQQSK
                     VGRDILFIERNLDFLKDGGRMAIVLPQGRFNNSSDKYIRDFIAERCRILAVVGLHGNV
                     FKPHTGTKTSVLFVQKWHDELCPKVEDYPIFFATMQKSSKDNSGDKIYVKNADGTLKL
                     DNHQHLIVEHDLFNHDNSTQDGIAEAFIEFAKKEKLSFVGKD"
     gene            1926..>2275
                     /locus_tag="PXD66_002396"
     CDS             1926..>2275
                     /locus_tag="PXD66_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL6320638.1"
                     /translation="MDSTFFLKKYLNHPSFNFPLSLNEVSLIKSGTTPTDRDDNLKEG
                     VVLLKTNDIRNNLLNKYSSVDYFISEDINEKMISSQLKEGDVLVNIVGATLEVVGRVA
                     YVSSTFPKANITQA"
BASE COUNT          788 a          353 c          462 g          672 t
ORIGIN      
        1 acacctgaag aagcagtacg ccaactctat ttaatgaatt tgcattatga atatgattat
       61 ccatttgaac gaatggaatt agaatattct gttgtattcg ggcgtgagaa gaaacgagcg
      121 gatattgtca tctttgatag agataatcct aacgctgtct atattatggt ggagctgaaa
      181 aagccgaaat taaaagatgg taaagaacaa ttaaaatctt attgtaatgc gactggagca
      241 cctattggtg tatggacaaa tggcgaaagt atttcttact ataatcgccg tgatcctaat
      301 tattttaaag atattcccga tattccgaat gctcaacaaa aactttctga tttattacaa
      361 gaacgttgga ctattcaaga tctgatagat aatgacaaaa ttgccaaaca acgtaaatca
      421 ttgaaagatc ttgttattga aatggaagat gaggtactgg cgaatgctgg agttgatgtt
      481 tttgaagagt tatttcaact tatttttact aaactctatg atgaaatgga aagtacacgc
      541 aataaaagtc gttatttaga gtttcgtaac tatggtgata ccgaaagtga attaaaggca
      601 aaaattcaat ctctatttga tcgtgcaaaa aataaatggg aaggcgtatt tgctgaaagt
      661 gccaaaatca acctttcccc ttcgcatttg gcgatttgtg tatcttcatt ggaagaagta
      721 aagctgttta attctaacct tgatgtagtg gatgaagcat ttgagtactt aattaataaa
      781 tccagtaaag gagagaaagg acaatacttt acgcctcgtt acgtcattga tatgtgcgta
      841 aaaatgctcg atccaaaacc tgaagaaaca gtcattgata ccgcagcagg aagctgtggt
      901 tttccagttc attctatttt tcacgtttgg gaaaaacaac ttaaagcgag aggattagaa
      961 cgctcacact tatttactgc tgaagaaaaa ttgcctgaac aaacagatta tgtgaaagaa
     1021 aaagtctttg cgattgattt cgatgaaaag gcagtacgtg ttgctcgtac cctaaactta
     1081 atcgcaggag atgggcaaac caacgtacta catttaaata ccttagatta tgaacgttgg
     1141 gaagatttta ctaaggaaga agaatggaat gatgtatatg gagaaggctg gaaaaaatta
     1201 agaaaattac gtaaaactaa aaatgaaaat cgagatttcc aatttgatgt gctaatggca
     1261 aatccgcctt ttgccggtga tattaaagaa actcgtatat tagctcgcta tgaattaggc
     1321 aaaaatagca aaggaaaaca gcaatccaaa gtcggacgag atattttatt tattgaacgc
     1381 aatttagatt tcttaaaaga cggtggacga atggcgattg tgctaccaca agggcgtttt
     1441 aacaattcca gcgataaata tattcgtgat tttattgcag agcgttgccg cattttagct
     1501 gttgtgggct tacacggaaa tgtgtttaaa ccacataccg gcacaaaaac ttccgtacta
     1561 tttgtgcaaa aatggcacga tgaactttgc ccgaaagtag aggattaccc gattttcttt
     1621 gccacaatgc agaaatccag caaagataat tcaggcgata aaatctatgt taaaaacgct
     1681 gatggcacac tcaaattaga caaccatcaa cacctaattg tggagcacga tctatttaat
     1741 cacgataata gtacccaaga cggcattgca gaagccttta ttgaatttgc taaaaaagaa
     1801 aagctctctt ttgtgggaaa ggattaagcc catttgaccc tgctaaatat caggcattgt
     1861 tagatgggct agaggtgagc gaggtttatt tgagtaaggt tcgttctgca agttattcgg
     1921 aacgaatgga tagtactttc tttttgaaaa aatacttaaa ccatccgagt tttaattttc
     1981 cattatcgct gaatgaggtt tctctgatta aaagtggtac aacaccaaca gatcgagatg
     2041 ataacttaaa agagggggtt gtcttattaa aaaccaatga tattaggaat aatctattaa
     2101 ataagtactc ttcggtagat tattttatta gcgaagatat caatgagaaa atgatttcat
     2161 cacaactgaa agaaggggat gttttagtaa atatcgttgg tgcaactttg gaggttgttg
     2221 gtcgggtagc ttatgttagt agcacttttc caaaagcaaa tattacacaa gcaat
//
LOCUS       DANLXF010000284         2219 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.285, whole genome shotgun sequence.
ACCESSION   DANLXF010000284 DANLXF010000000
VERSION     DANLXF010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.285"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..609
                     /locus_tag="PXD66_002397"
     CDS             <1..609
                     /locus_tag="PXD66_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HDL6320639.1"
                     /translation="AEEAKKIAEAKGIGNKEYKDGLNELEEKNETGKNGEEIRTHGYL
                     YNSHYSVVTAKMKQTLQENGRQMEPTVEVKGLKTENLPTEGKATYKGEAFDSHGNNSN
                     SVVGGELIYNVDFSSRTGSGLVKNVQGGSIELAQGEIKNDSIIASAHQKYNDQAVGNG
                     SYNIQFFGPNAEEIGGKIELNGEGEGSMKQMLGIAGTREEQK"
     gene            658..2052
                     /locus_tag="PXD66_002398"
     CDS             658..2052
                     /locus_tag="PXD66_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL6320640.1"
                     /translation="MYKKHFLFLLVSPLVMAETVEFQQDPKEFDGQRQAVKFEQEIAK
                     KDLKLTAYLEEDNSLKGIEQHINQAILRQDWAKLERLLGDYAKQEQFDPLLLDYALAA
                     LAYAKKDYRSAAFYYQQLLKKQPDLTYPRFDLALILKEDYRYREAEQEFKQLKTDDLR
                     NAVNSALAQIEEEESWKPDFYLQYTQTNNVNNASSSPTVNVNGRIFIKESDSLPQSAK
                     GVRYGVGLSKMRNIGGNHFVGVDLGYNGVYYWNNHDYSEQSVSLTPKYSYRTATYRIG
                     VSPFIEQNWLGSSRYNYQFGSSINAVKLFERGLISGSFSHLQKRYSEQHIADRYNGYR
                     NQLTLGTQWQAVKNWRFFANLNASREITREKAQSSNKWLATIGVIYQTPSWASSVSVG
                     YGKRYFADKHYLFGYKRQDKEYQANVAVWNRSWHWQGIVPKLNFRYQKIDSNIADFYS
                     RSNKEVFMTLEKLF"
BASE COUNT          743 a          378 c          498 g          600 t
ORIGIN      
        1 gctgaagaag caaaaaaaat tgcagaggca aaaggaattg gcaataaaga atataaagat
       61 ggattaaatg agttagaaga aaaaaatgaa acaggtaaaa acggagaaga gattcgaacc
      121 catggttatc tctataacag ccattattcc gttgtaactg ccaaaatgaa acaaacttta
      181 caggaaaatg gtcgtcaaat ggaacctacg gttgaagtga aagggcttaa aactgagaat
      241 ttaccgacag agggcaaagc aacatataaa ggagaagcct ttgattctca cggtaataac
      301 agcaattcag tagtaggtgg agaattaatc tataatgttg atttctctag tcgtaccgga
      361 tcaggtctag ttaaaaatgt acagggcggt tctatcgagt tagcacaagg cgaaataaaa
      421 aatgatagta ttatagcttc tgctcaccaa aagtataatg atcaggctgt tggaaatggt
      481 agttacaata tccaattctt tggtccaaat gcagaagaaa ttggagggaa aattgagctt
      541 aatggtgagg gcgaaggctc aatgaagcag atgttaggta ttgcaggaac ccgagaagaa
      601 caaaaataac atttaatacc caatgccgtt taatcttaaa cggcattctt ttttactatg
      661 tataaaaaac attttctatt tttacttgtt tcgcctttag taatggcgga aacggttgag
      721 tttcagcaag atcccaaaga gtttgatggg cagcgacaag cggttaaatt tgagcaagaa
      781 attgcaaaaa aggatttaaa attaaccgct tacttggagg aagataattc tcttaaaggt
      841 attgaacagc acattaacca agcgattctt agacaagatt gggcgaagct ggaaaggctg
      901 cttggcgatt atgcaaagca agagcaattt gatccgctac ttttagacta tgctttggcg
      961 gcattggctt atgctaaaaa agattatcgc tctgcggcgt tttattatca gcagttgctg
     1021 aaaaaacagc ctgatttaac ctaccctcgt tttgatttgg ctctgatttt aaaagaggat
     1081 tatcgctaca gggaagcaga gcaagaattt aagcaactca aaaccgatga tttacgcaat
     1141 gcggtgaaca gtgcgttggc tcaaatagag gaggaagaga gctggaagcc ggatttttat
     1201 ttgcaatata ctcaaaccaa caatgtgaat aatgcttctt cgtcgccaac ggtaaatgta
     1261 aacgggcgga tttttattaa agaatcagac agcttgccgc agtcggcaaa aggcgtaaga
     1321 tatggcgttg gcttgagtaa aatgcgaaat atcggcggta atcattttgt gggagtcgat
     1381 cttggctata acggcgttta ttactggaat aaccacgatt atagcgaaca gtcggtctca
     1441 ctaacaccaa aatatagcta cagaacggca acttatcgta tcggggtttc gccctttatt
     1501 gagcagaatt ggctgggtag ctcacggtat aactatcaat ttggtagcag tatcaatgct
     1561 gttaaacttt ttgaacgagg gctgatttcc ggctcattca gccatctgca aaaacgttat
     1621 agtgaacagc atattgccga ccgttacaat ggttatcgca accaactcac gctaggtacg
     1681 caatggcaag cggtcaaaaa ttggcgattt tttgcaaatc ttaacgctag ccgtgaaatt
     1741 acccgtgaga aagctcaatc ttccaataag tggctggcga ccattggcgt aatttaccaa
     1801 accccaagct gggcaagttc ggtaagtgtc ggatatggca agcgttattt tgctgataaa
     1861 cattatctgt ttggctataa acgccaagat aaggaatatc aagcgaatgt ggcggtgtgg
     1921 aatcgaagtt ggcactggca agggattgtg ccgaaattga atttccgtta tcaaaaaatc
     1981 gatagcaata ttgccgattt ctactctcgt agcaataaag aagtctttat gaccttagag
     2041 aaactatttt aaggctgtgt atgaatgtcg gggagaaaaa ttttctcccc gatatttatg
     2101 tgtagaagat cagacttgat ctgacaattc actctaaaaa gtgagagttt cccgtttaga
     2161 atatgagtgt caaaaatcaa tctaaacaaa aaggaaactc tcatgtttta ttctaacaa
//
LOCUS       DANLXF010000285         2213 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.286, whole genome shotgun sequence.
ACCESSION   DANLXF010000285 DANLXF010000000
VERSION     DANLXF010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.286"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            31..1626
                     /locus_tag="PXD66_002399"
     CDS             31..1626
                     /locus_tag="PXD66_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HDL6320641.1"
                     /translation="MQRHEQLAQVQQTQKWDFIIIGGGASGLGIAVDAASRGYKTLLL
                     ESHDFAKGTSSRSTKLVHGGVRYLANGDVALVREALRERGRLAKNAAHLFKNQSFIIP
                     NYKFKDCVMYRVGLGLYDMLSGNLSLGKTENISKTEAKRRLPTLNDTGLRTGTVYQDG
                     QFDDSRLAVNMAQTVVEHGGTVLNHAKVTSLHKTPQGKIDGVTFYDELNQQEHTVFGT
                     AVINATGVFMNDILSMDHGSTKKFVVPSQGIHLVLDKSFLPSNDALMIPKTSDGRVLF
                     AVPWHEVLVVGTTDTLIENPSYEPIPLEQEVNFILETAAQYLTKKPTRDDVKAVFAGQ
                     RPLAAPEKEGQSTKEVSRSHKVLTDATSGLITITGGKWTTYRQMAEDTVEEALKVHSE
                     LGNKPCVTVNLPIHGNIPAAQVNLQDHLYVYGSDIPQLRALMTETPEYAQKIHPRLDY
                     TVAEVIWAVRHEMAQTVEDVLARRVRLLFTDARAAIDSAAKVAAIMAKELGKDEMWQQ
                     QQVIQFLDVAKHYLLVDYQPQPI"
     gene            complement(1713..2012)
                     /locus_tag="PXD66_002400"
     CDS             complement(1713..2012)
                     /locus_tag="PXD66_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320642.1"
                     /translation="MENIKMEELKLTEKELLVLKGNALRMRFKAETNKTKNEIMQPFK
                     QVKQTGLALVNSPAVKTIALDFLVKRLLSMKGISFSVLGLTAFFLLQQKHKRDDS"
     gene            complement(1999..>2213)
                     /locus_tag="PXD66_002401"
     CDS             complement(1999..>2213)
                     /locus_tag="PXD66_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDL6320643.1"
                     /translation="GLDNYLLPEQKVKVFFSIAIIMFIAVTICGMMILSALKKQRHFM
                     QSTLTELKLDIAAFKSALTLKQNGEH"
BASE COUNT          716 a          421 c          445 g          631 t
ORIGIN      
        1 atgttttaat tcgagcaaaa ggagcaaaaa atgcaaagac atgaacagct agctcaagta
       61 caacaaacgc aaaaatggga ttttattatt atcggtggtg gcgcaagtgg tttggggatt
      121 gcggtagatg cagcttcccg tggctataaa accttattat tagaaagcca tgactttgcg
      181 aaaggtacat cgagccgaag cacaaaatta gtccacggtg gcgttcgcta tcttgcaaat
      241 ggcgatgttg cactcgtgag agaagcattg cgtgaacgag gtcgtttagc taaaaatgca
      301 gctcatttat ttaaaaacca aagctttatt attccaaact acaaattcaa agactgtgta
      361 atgtatcgtg ttggtttagg cttatacgat atgctttcag gcaacctgag tttaggaaag
      421 actgaaaaca tcagtaaaac tgaagcgaaa agacgtttac caaccttaaa tgatactggt
      481 ttacgtacag gcactgttta tcaagatggg caatttgatg actcacgtct tgcagtgaat
      541 atggcacaaa cggtagtaga gcacggcggt acagtactaa atcacgccaa agtgacttca
      601 ctgcacaaaa cgccacaagg caaaattgat ggggtgactt tctacgatga actcaatcaa
      661 caagaacata ctgttttcgg cactgcggtg attaacgcaa ccggcgtatt tatgaatgat
      721 attttaagta tggatcacgg tagcacgaaa aaatttgtcg taccaagcca agggattcac
      781 ttagtattag ataaatcatt cttaccaagt aatgatgcgt taatgattcc aaaaacctct
      841 gacggacgag tgttatttgc tgtaccttgg cacgaagtgt tagtcgtagg gacaacagat
      901 actctgattg aaaatccttc ttatgagcca attccattgg aacaagaggt gaactttatt
      961 ttagagacag ccgcacaata tctgacaaaa aaaccaactc gtgatgatgt aaaagcagtc
     1021 tttgccggtc aacgtccgct tgcggcaccg gaaaaagaag gtcaaagcac taaagaagta
     1081 tctcgtagtc acaaagttct cactgatgca accagcggtt tgattacgat tacaggtggt
     1141 aaatggacaa cttatcgtca aatggcagaa gataccgtag aagaagcgtt aaaagtccac
     1201 tctgaactag gcaataaacc ttgtgtgacc gtcaacttac cgattcacgg taatattccg
     1261 gcagcacaag tgaatctaca agatcatcta tatgtatatg gctcagatat tccacagtta
     1321 cgcgccttaa tgactgagac cccggaatat gcacaaaaaa tccatccgag actagattat
     1381 accgttgcag aagtgatctg ggcagttcgc cacgaaatgg cacaaacggt tgaggatgta
     1441 ttagctcgcc gtgttcgttt gctcttcact gatgcgagag cggcgattga tagtgcagca
     1501 aaagtggctg ctattatggc gaaagaatta ggtaaagatg aaatgtggca acaacagcaa
     1561 gttatccagt tccttgatgt tgcgaagcac tatttattgg tagattatca gccacagcca
     1621 atctaatacg ctaaaagcaa gcggttaaaa tcgataaaaa tattgcaatt acgttatttg
     1681 caacttttta aaccatttta accgcttgta tattagctat catctctttt atgtttctgt
     1741 tgtaataaaa agaatgctgt taaccccaat acagaaaagc taattccttt catactcaat
     1801 aatcttttta ccaaaaaatc taatgcaatg gttttaactg ccggtgaatt gaccagtgca
     1861 agccctgttt gtttcacttg tttaaaaggc tgcataatct catttttggt tttattggtt
     1921 tccgctttaa atcgcattct aagtgcatta ccttttagaa ctagaagttc cttttcggtt
     1981 aatttaagct cttccatttt aatgttctcc attctgtttc aaagttaaag cactcttaaa
     2041 tgcggcaata tctaatttta gctcggttaa tgtactttgc ataaaatggc gttgtttttt
     2101 caaggcagaa agtatcatca ttccgcaaat agtcacagca ataaacataa taatggcaat
     2161 agaaaagaat actttaactt tctgttcggg taacaaataa ttatctaaac caa
//
LOCUS       DANLXF010000286         2190 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.287, whole genome shotgun sequence.
ACCESSION   DANLXF010000286 DANLXF010000000
VERSION     DANLXF010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.287"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            166..1311
                     /gene="lldD"
                     /locus_tag="PXD66_002402"
     CDS             166..1311
                     /gene="lldD"
                     /locus_tag="PXD66_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent L-lactate dehydrogenase LldD"
                     /protein_id="HDL6320644.1"
                     /translation="MIISSANDYRRAAKRRVPPFMFHYADGGSYAEQTLRRNVSDLED
                     IALRQRVLKDMSQLDTGIELFGEKLSMPVTLAPVGALGMYARRGEVQAAKAADNKGIP
                     FTLSTVSICPIEEVAPAIKRPMWFQLYVLKDRGFMKNALERAKAAGCSTLVFTVDMPT
                     PGARYRDMHSGMSGPYKDIRRVLQAMVHPFWAWDVGIHGKPHTLGNVSNYMGKPIDLN
                     NYIGWLTDNFDPSISWKDLEWIREFWDGPMVIKGILDPEDAKDAVRFGADGIIVSNHG
                     GRQLDGVLSSAKALPSIADAVKGEIKILADSGIRNGLDVVRMLALGADCTMIGRSFVY
                     ALSAAGQAGVENLLDIFLKEMKVAMTLTSNAKISDIGRDALVDLSNL"
     gene            complement(1365..1634)
                     /locus_tag="PXD66_002403"
     CDS             complement(1365..1634)
                     /locus_tag="PXD66_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320645.1"
                     /translation="MLSEERFGFFYIFIFIYFSLLSLSIGYIRPELRTTYDYLEIENK
                     RYYVLSIHRNNIYVLGEKTKDNDEFLFFNQDTLKYYRINITKMPN"
     gene            complement(1709..2095)
                     /locus_tag="PXD66_002404"
     CDS             complement(1709..2095)
                     /locus_tag="PXD66_002404"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320646.1"
                     /translation="MTNQKFINSSLTLAFFTLISFSTAYCYAWGIAAFHGYPWWHVEV
                     GNAGIARALAYVFGTFLTIFLFYLIGYALVNKVFKLHYFKYLGWLRVSVLVTIFSLPV
                     MISFYLFIGKVPMYLHLLYLGTTTSI"
BASE COUNT          651 a          438 c          494 g          607 t
ORIGIN      
        1 atcaattgtt ttgttggttg ctttttgtgc aattgtattt ttatgctaat tggtttgtaa
       61 caaaaatgtg atctatttct catattttgc aaatagtagt aattcttatt tttattttaa
      121 agtagaatag ccgcactttt ttcttaatga tttatgaggt tttaaatgat catttcatct
      181 gcaaacgatt accgccgtgc ggcaaaacgt cgtgttcccc cttttatgtt ccattatgcg
      241 gatggcggtt cttacgccga gcagacctta cgtcgtaatg tgagcgattt ggaagacatc
      301 gccctgcgtc aacgtgtgtt aaaagatatg tcgcaactag acaccggcat cgagctgttc
      361 ggcgaaaaac tgtctatgcc ggtgaccctc gcccctgtcg gtgcattagg aatgtatgcc
      421 cgccgtggcg aagtgcaagc ggccaaagcc gcagataaca aaggtattcc gtttaccctt
      481 tccactgtct ctatttgccc gattgaagag gttgcaccgg caatcaaacg cccgatgtgg
      541 tttcagctct atgtgttaaa agatcggggc tttatgaaaa atgcattgga acgagccaaa
      601 gccgccggtt gttccacgct agtgtttacc gtagatatgc cgacccccgg cgcacgttat
      661 cgtgatatgc actccggaat gagcggccca tacaaagaca ttcgccgtgt gttgcaggca
      721 atggttcacc cgttttgggc gtgggacgtg ggcattcacg gtaaacccca cacgttaggc
      781 aatgtgtcaa actatatggg taaaccgatt gatctgaaca actacatcgg ctggctgacc
      841 gataatttcg acccatcaat ttcgtggaaa gatttagagt ggattcgtga attttgggac
      901 ggcccgatgg tgatcaaagg cattctcgac ccagaagatg ctaaagatgc cgtaagattt
      961 ggtgcggacg gtatcatcgt ctccaaccac ggtggtcgtc agttagatgg ggtactttct
     1021 tctgccaaag ctctgccaag cattgccgat gcagtaaaag gggagatcaa aatccttgcc
     1081 gactctggta tccgcaacgg cttagatgtg gtgagaatgc tggctctcgg cgcagattgt
     1141 acgatgatcg ggcgttcctt tgtttacgct cttagcgccg ccggtcaagc cggtgtggaa
     1201 aacctgctcg acatcttcct taaagaaatg aaagtggcga tgacacttac cagcaatgct
     1261 aaaattagcg acatcggcag agatgctttg gtagatttaa gtaatcttta gctcaaatag
     1321 ccacaatccg cacgttttag gtgcggattg ttattttaga taggctaatt tggcattttg
     1381 gtaatattaa tgcggtaata tttcaatgta tcttggttga aaaagagaaa ttcatcgtta
     1441 tctttagttt tttcgcccag cacataaatg ttattgcgat gaatagataa gacataatat
     1501 cgcttatttt caatttcaag ataatcatac gttgttctca gctcagggcg aatgtagcca
     1561 atactcaaag aaagtaagct aaaatagatg aaaataaaaa tatagaaaaa accgaagcgt
     1621 tcttcgctta acattttttg aatatctaag tggaatacgc ggtggcccca atgtttgtga
     1681 aataataata ctgatagggt agttgtccct agatagaggt agttgtccct agatagagta
     1741 ggtgtaaata cattggcact ttgccaataa acagatagaa actgatcatt actggcaagc
     1801 taaaaatagt aacaagaaca ctcacccgta gccagccaag gtatttaaag taatgcagct
     1861 taaaaacttt attaactaaa gcgtatccta tcaaataaaa aagaaagata gttaaaaatg
     1921 tgccaaaaac gtaggctagt gcgcgagcta taccggcatt accaacctca acatgccacc
     1981 acgggtagcc atgaaaagca gcaatacccc aagcataaca gtaagcggta gaaaaagaga
     2041 ttaaagtaaa gaaagcgaga gtaagagaag agttgatgaa tttctgatta gtcataacag
     2101 taaatagatt gcacctactt agttaaagat gaaacacaag aaagcagact gagtttagct
     2161 tccacccaac tcctttgttt agaggagaac
//
LOCUS       DANLXF010000287         2185 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.288, whole genome shotgun sequence.
ACCESSION   DANLXF010000287 DANLXF010000000
VERSION     DANLXF010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.288"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            66..1997
                     /locus_tag="PXD66_002405"
     CDS             66..1997
                     /locus_tag="PXD66_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL6320647.1"
                     /translation="MIFFTDLILKRGHSVLIDNSSVTIHTGQKVGLVGKNGCGKSSLF
                     ALLKNELQPEGGDVSLPKNWAISWVNQETPALAISALDYVIQGDREYTHLMAELDIAN
                     EKNDGNRIAIIHTQLDTIDAWTIQSRAATLLNGLGFSTEQLQLPVKSFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLERWLSNYRGTLLLISHDRDFLDPIIDRVIH
                     IEHQKLNEYTGNYTSFEIQRATKIAQQNAAYQQQQRKVAHLQSFIDRFKAKATKAKQA
                     QSRIKALEKMELIAPAYADSPFEFEFRPPLALPSPLLMMEKVSAGYAEKTVLESVKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELPPQNGSIQLAKGVQLGYFAQHQVDTLRFDE
                     SPLWHLQKLAPEKTEQELRNYLGGFDFKGDKVVQAVKSFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLEMRQALVDALTYYEGSLVVVSHDRHLLRSTVNEFYLVHDRKVEKF
                     KGDLDDYQKWLNEQNALANEPKSVLAKDSANSTACSSTNENSAAQRKEQKRLEAERRQ
                     QTAPLRKKITQLEKELEKATEKLNKLEAMLTESDIYEAENKAKLTETLAKQLEAKRLV
                     EEIEMQWLEAQEEFETMLR"
BASE COUNT          688 a          467 c          476 g          554 t
ORIGIN      
        1 aaagcaatgg ctttgctttt ttatgctaaa atacgcaaaa tttttaacaa aatagaccgc
       61 ttgcaatgat cttttttaca gacttaattt taaaacgagg gcattccgtt ctcattgata
      121 acagctccgt taccattcac accgggcaga aagtcggttt ggtgggaaaa aacggctgtg
      181 gcaaatcctc tctttttgcc ttactgaaaa atgaattaca acctgaaggt ggcgatgtat
      241 cgttgccgaa aaactgggcg atctcgtggg taaatcaaga aacgcctgcg ttagctattt
      301 cagcgttaga ctatgtgatt caaggagatc gggaatatac ccatcttatg gctgagctag
      361 atatcgcaaa tgaaaagaat gacggcaacc gcattgcgat tatccatact caacttgata
      421 ccattgatgc ttggacaatt caatcccgtg ccgctaccct gcttaacggc ttggggttta
      481 gcaccgaaca actgcaactg ccggtgaaat ccttctccgg tggctggcgt atgcggttaa
      541 atttggcaca agctctgatt tgtcgttccg atctgttatt gcttgacgag ccaaccaacc
      601 acttagactt agatgcggtt atttggttgg agcgttggct gagtaactat cgtggcactt
      661 tattgttaat ttcgcacgac cgtgattttc tcgatccgat tatcgatcgg gtgattcata
      721 ttgaacatca aaagctcaat gaatacaccg gcaattacac ctcgtttgag attcaacgtg
      781 cgaccaaaat cgcccagcag aatgccgctt atcagcaaca gcaacgtaaa gttgctcatt
      841 tgcagagttt tatcgatcgc tttaaagcca aagcgactaa agcgaaacag gcacaaagcc
      901 gaattaaagc gttggaaaaa atggagctta ttgccccagc ctatgctgat tcgccgtttg
      961 aatttgaatt tcgcccaccg cttgcactgc ctagcccgtt attaatgatg gaaaaagtca
     1021 gtgccggcta tgcggaaaaa acggtgttgg aatcggtcaa actgaatttg gttcccggct
     1081 ctcgtattgg tttactcggg agaaacggtg cagggaaatc cacgctgatt aaattattgg
     1141 cgggcgaact tccgccccaa aacggctcaa ttcaactggc aaaaggcgtg caactcggct
     1201 atttcgccca acatcaggtg gatacactac gttttgatga aagcccactt tggcatttac
     1261 aaaaactcgc accggaaaaa accgagcagg aattacgcaa ttatttaggc ggtttcgatt
     1321 ttaaaggcga taaagtggta caagctgtga aatcattctc cggcggagaa aaggcccgtt
     1381 tagtgcttgc cttaattgta tggcaacgcc cgaatttact gctattagat gagccaacca
     1441 accatttaga tttagaaatg cgacaagccc tggttgatgc actgacctat tacgaaggct
     1501 cgctagtggt ggtttcgcac gaccgccatt tattgcgtag caccgtgaat gaattttatt
     1561 tagtgcacga ccgcaaagtg gaaaaattta aaggtgattt agacgattac caaaaatggc
     1621 tcaatgagca aaacgcctta gccaacgagc cgaaatccgt acttgcaaaa gattcggcaa
     1681 attcgaccgc ttgctctagc accaatgaaa atagtgcagc tcaacgcaaa gagcaaaaac
     1741 gcttagaggc agagcgccgc caacaaaccg ccccattgcg gaaaaaaatt actcaactgg
     1801 aaaaagagtt agaaaaagca accgaaaaat taaataaact cgaagcaatg cttaccgaaa
     1861 gcgacattta tgaagccgaa aataaagcaa aactaactga aacacttgcg aaacagttag
     1921 aggcaaaaag gctggttgaa gaaattgaaa tgcaatggtt ggaggctcag gaggagtttg
     1981 agaccatgtt acgctagggt tctacaagat agcgcaagcg tccacgcttg tgccatttaa
     2041 gggaactata cacctcaaaa aaacaatgat cattaagcta gcttttttat aaatttaaaa
     2101 acctatagat taaataaata atgatattaa tagtaaaatc acgagtgcaa ctacactgac
     2161 gataataaaa tcaggcatac ttatt
//
LOCUS       DANLXF010000288         2175 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.289, whole genome shotgun sequence.
ACCESSION   DANLXF010000288 DANLXF010000000
VERSION     DANLXF010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.289"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..293)
                     /locus_tag="PXD66_002406"
     CDS             complement(<1..293)
                     /locus_tag="PXD66_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320648.1"
                     /translation="MEAKYNMIIELAQQQGLTHIQLTQKHCLHLAASVGKLCSGVVNN
                     AKQKIKTATGEIFTLLTIINHQIQFDEDLELHTWKASGTTPPEQRAIRMLHHTS"
     gene            complement(605..680)
                     /locus_tag="PXD66_002407"
     tRNA            complement(605..680)
                     /locus_tag="PXD66_002407"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Arg,seq:tct)
     gene            961..1233
                     /locus_tag="PXD66_002408"
     CDS             961..1233
                     /locus_tag="PXD66_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014391457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas2"
                     /protein_id="HDL6320649.1"
                     /translation="MRNYLISYDLYKAGQNYDGLISYIKSHQVWAAIHKSVWYIKSNK
                     SAEQIRNELLIYLDKNDSVFVTDMNSAAWNNLTEGNGEYIRQNWMR"
     gene            complement(1211..1480)
                     /locus_tag="PXD66_002409"
     CDS             complement(1211..1480)
                     /locus_tag="PXD66_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320650.1"
                     /translation="MKNKNFEIAKVVILNEQLGSPKDLELKVVENSLSDEDRYDIKQA
                     VLKAVGNGCSDPEMLASRCCSAIEIINRNGLNTGNGSISTSSNSV"
BASE COUNT          601 a          414 c          415 g          745 t
ORIGIN      
        1 gaggtatgat gtaacatacg tatcgctctt tgctcgggtg gggttgttcc actagctttc
       61 cacgtgtgaa gctctaagtc ttcatcgaac tggatttgat ggttaatgat ggtgagtaat
      121 gtaaaaattt ctcccgtagc cgttttaatc ttctgtttcg cattattaac gactccagaa
      181 catagtttcc ccactgatgc agcaagatga agacagtgtt tttgagtaag ttgaatatgg
      241 gttaagcctt gttgttgtgc cagctcaata atcatgttgt atttagcttc catttttcgt
      301 tcctttttta gtcaaaaatc tgaattttgg gtgtaaaaag cccctccgca gtaattactt
      361 aaccttgagc aactctacag gcgttatatt aaggagatgt ttctgatggc ggatttaatt
      421 cttgaggagc aatccaatca tcgctaactt cccagatttc ttcttgccct gcttgttcag
      481 ggctttttaa cttaataagc atttgatacc tcacattgtt ttattagcca ttcaaaaccg
      541 ctctacacgg taggtttcca cttcgtaggc tcgtcatcga tgatgtgcgc aaagcggttt
      601 tggatggcga ccgcagagag attcgaactc tcgacccact gcttagaagg cagctgctct
      661 atccactgaa ctatgcggtc aaagtaccgt tgcttaaccc caacggcagg gtttacttca
      721 atctaaggag gatggctttc accatctcaa accgctctct agtccattcg tttcgctgga
      781 taacggcact ttgggaaaac atacgcaaaa aaccaagtga ggttatcggg ctaactcttt
      841 gttgagagcg gtttgagatg gtggctcttt tatacttgtg agccgtcaag tttccttatt
      901 gtctctcaca acactaagga atataattaa atctctcaca gcacaaataa ggattcttct
      961 atgagaaact atttaatttc ttacgattta tacaaagctg ggcaaaatta cgatggattg
     1021 attagttata ttaaaagcca tcaagtttgg gctgctattc ataaatcagt ttggtatatc
     1081 aaatctaata aatctgcaga acaaattcgt aatgaactgc tcatttattt agataagaac
     1141 gatagtgtct ttgttacgga tatgaatagt gctgcttgga ataatctgac tgaaggaaat
     1201 ggtgaataca ttagacagaa ttggatgagg tagatatact cccattacca gtgtttagcc
     1261 catttctatt aattatttcg attgcggaac aacatcgtga tgcaagcatt tctggatctg
     1321 aacaaccatt ccctacagcc ttaagtaccg cctgtttgat gtcgtatcta tcttcatcag
     1381 ataggctatt ctctactact tttaattcca aatctttagg gcttcctagt tgttcattta
     1441 aaataacaac tttcgcaatt tcaaaatttt tgtttttcat aatttacctc tagttaaata
     1501 ttccaaaaca cactttctca aatatgcttt ggaatttgaa atgccgtctc tccgagctgt
     1561 cacgcaattt tagagctgcg tttgcacaat ttgaatttcg gttactccct tgtgtctcaa
     1621 tgtacacgcc cagactttca cctaaaccgc tataagggta tatgcttgta ttcctttgct
     1681 tgcaccaaag ggcataacgc tttgaagtag tgggcgtttt aagggctgcc tccgactttc
     1741 gtcttcctac ttttcaccat taggcttaaa gcggttttga atttgatttt taaagagcat
     1801 tgagccgtag ctcgttttga tgggtgcatt ataccttatg gtaaatattg ttgtaaatac
     1861 caaaaggtat gttaaataag gtaaaaataa ataacaaaaa ggtaatttgt tgattttaaa
     1921 aggaatttat ttttagaaag gttgtttgat tgcttaataa ataaacagat tgagttgcaa
     1981 gcggtcgttt ttgaagagaa ttttgcagat tttaggcaag agaaaaccgc cctcctggcg
     2041 gtttattcaa ggaatattat tttatctttt gaattgttgt agtaacagat cgagtttatt
     2101 atcaatccca tctattttct tatccatatt gcctagtcgc tgctcaacat tgtctagtcg
     2161 ttgttcgaca ttatc
//
LOCUS       DANLXF010000289         2088 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.290, whole genome shotgun sequence.
ACCESSION   DANLXF010000289 DANLXF010000000
VERSION     DANLXF010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.290"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            46..330
                     /locus_tag="PXD66_002410"
     CDS             46..330
                     /locus_tag="PXD66_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320651.1"
                     /translation="MLLEQFGITGKLAEDFIVHRKAKKAPITETALNGYQREADKAKI
                     PIQKAVEIAIERGWTGFKADWQWQDDQPKHRPKDNMRAEWNNPEAWAEVF"
     gene            330..977
                     /locus_tag="PXD66_002411"
     CDS             330..977
                     /locus_tag="PXD66_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDL6320652.1"
                     /translation="MSLQKTEQNRPLAVLGEQQGKLSGEVESLVDRIFDQLLASCPSI
                     QYWSEKQVATAKKQWILGFAENGIRTVEQVRQGMKALRAKTDDFVPSIGKFIQWCNTI
                     DYHELGLPDLEQLLKLLDRSLRFDFIEIQKLKFKSNAEYWLITDLYARNKKYSWKDET
                     LRNQAEKALVAMAKRIMSGEQIPPPKPLIEEKKKVPIDPRIQRILDEKKQAGAMQ"
     gene            974..1546
                     /locus_tag="PXD66_002412"
     CDS             974..1546
                     /locus_tag="PXD66_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine methyltransferase"
                     /protein_id="HDL6320653.1"
                     /translation="MTDFDKDTYQTPKYFRNWLNHRYAWFHIDGCSNGKNALYSYWIG
                     KAAEGLDEDTLSGQIAEDFLAENLLDVILERVADWGELLRIFVNPPYSNPLPFVQRAA
                     ELKKAGHIVVMLLPADKSTRWYQVIQENASEVIDIIGGRINFINPLTGEEVKGNNKGS
                     MVAVFDPFVEGIVTRQMPLDKIKELGGYEK"
     gene            1665..>2088
                     /locus_tag="PXD66_002413"
     CDS             1665..>2088
                     /locus_tag="PXD66_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HDL6320654.1"
                     /translation="MAKYQMMKLPGGILSPLNDEEAERLKSFQNGEQYEIEIKRQRNP
                     AFHRKVFAFFNFCFDCWSGSHSDWEFQDPTAQFDTFRKHLTVMAGFYVQTWNLKGQVR
                     IEAKSLSYANMEQDEFEQAYKALINAAIKHVFNNTKDEN"
BASE COUNT          648 a          384 c          530 g          526 t
ORIGIN      
        1 ggtattaacg cacacacacg tgagactaaa aaatctgctg tgttggtgtt gcttgaacaa
       61 ttcggcatta caggcaaatt ggcggaggat tttatcgtac accgcaaagc caaaaaagcc
      121 ccgattaccg aaactgcact gaatggctat cagcgagagg cagacaaagc caaaatcccg
      181 attcaaaagg cggtagaaat tgcgattgaa cgtggctgga caggatttaa agccgattgg
      241 cagtggcaag acgatcaacc taaacatcgc ccaaaagaca atatgcgagc tgaatggaat
      301 aaccctgaag cgtgggcgga ggtgttttga tgagcctgca aaaaacagag caaaatcgac
      361 cgcttgcggt gttaggtgag cagcaaggca aattatccgg tgaggttgaa agccttgtgg
      421 acagaatatt tgaccagttg cttgcgagtt gcccaagtat tcaatactgg tcggaaaagc
      481 aagttgccac agccaaaaaa caatggattt taggctttgc ggagaacgga atccgcacgg
      541 ttgagcaagt tcgacagggt atgaaagcgt taagagcaaa aactgatgat ttcgtcccga
      601 gtatcggcaa atttatccag tggtgcaata cgattgacta tcacgaatta ggattacctg
      661 atttagagca gttactcaag cttttggatc gctctttaag atttgatttt attgagattc
      721 agaaacttaa atttaaatca aacgctgaat attggttgat aactgaccta tacgcacgta
      781 acaagaaata tagttggaaa gatgaaacct tacgtaatca agcagaaaaa gccttagtgg
      841 caatggcaaa acgaattatg agcggagagc aaattccacc acctaaaccg ctaatcgaag
      901 agaaaaagaa agtgccaata gatccacgca ttcagcgaat tttggacgag aagaaacaag
      961 caggagcaat gcaatgacag attttgataa agacacttac caaacgccaa agtatttccg
     1021 taattggcta aatcatcgct atgcgtggtt tcacattgac ggttgttcta acggcaaaaa
     1081 cgcattgtat agctactgga ttggcaaagc ggcagaaggc ttggacgaag acacgctaag
     1141 tggtcagatt gccgaggatt tcttggcaga aaacttgctc gatgtgatct tggaacgtgt
     1201 ggcagattgg ggcgaattac tccgtatctt cgttaatccg ccatatagca atccactccc
     1261 ttttgttcag cgtgctgcgg agctgaaaaa agccggtcat attgtggtaa tgctgttgcc
     1321 ggcagacaaa tctactcgtt ggtatcaggt tatccaagaa aacgctagtg aggtgatcga
     1381 cattatcggt gggcgtatta attttattaa tccgctcaca ggcgaggaag tgaaaggcaa
     1441 caacaaaggc tcgatggtag cggtgttcga cccgtttgtg gagggaatag ttacccgtca
     1501 aatgccgctg gataagatta aggagctggg cggctatgaa aaataacgaa gggtgggaag
     1561 tgtgatgagt gattctacta ttggattttt agggattcta attttcttcg ggtggttagc
     1621 atatttggta tttagagctt ttggattttc attgagggat agagatggct aaataccaaa
     1681 tgatgaaatt gccgggtggg attttaagcc cgttaaacga tgaagaagcc gagcggttga
     1741 aatcgtttca gaatggcgag cagtacgaaa tcgagattaa acgccagcgt aatcccgctt
     1801 ttcatcgcaa ggtgtttgcg ttctttaatt tctgttttga ttgctggagt ggtagccatt
     1861 ccgattggga gttccaagat ccaaccgctc aatttgatac tttccgcaag catttaactg
     1921 taatggcagg gttttatgtg cagacgtgga atttaaaagg gcaagtgcgg attgaggcga
     1981 aaagcctaag ctacgccaat atggaacaag acgagttcga gcaggcttat aaagcgttga
     2041 ttaatgcagc tatcaagcac gtttttaaca ataccaaaga tgagaata
//
LOCUS       DANLXF010000290         2077 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.291, whole genome shotgun sequence.
ACCESSION   DANLXF010000290 DANLXF010000000
VERSION     DANLXF010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.291"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            166..606
                     /locus_tag="PXD66_002414"
     CDS             166..606
                     /locus_tag="PXD66_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3577 domain-containing protein"
                     /protein_id="HDL6320655.1"
                     /translation="MTTQTSTQKNYFNLHTTGIGYLSNIRTVTPKKGDEFLACTIGAL
                     IGEAGEGAKAEYRYFDCKVVGEAAIDLVNRAKASVEADKKVLISFVLADLWTDTFTYQ
                     KDGKNHKKGDTGVMLKARLIRIKSVKIDGVVKYQEPEKIDAQSE"
     gene            713..1360
                     /locus_tag="PXD66_002415"
     CDS             713..1360
                     /locus_tag="PXD66_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HDL6320656.1"
                     /translation="MTINTYAKFAPTVFVAKCPEAHTKGEIITLTSKYGNETEVQIHN
                     LVKQDNEHYYYSFTRCDGMDSQSFAQQKAERYQGYADNATKRSQSWANAANEGLEFLR
                     LGEPIKIGHHSEKRHRNLIERNARRMDNALSEMNKTESYESRIAYWEKMAGKIDLSMP
                     ESLEYFKFELDKAKEKHKELKDNPEKREHGYSLTYAKKAVNELEKKVKLAELLWA"
     gene            1384..1791
                     /locus_tag="PXD66_002416"
     CDS             1384..1791
                     /locus_tag="PXD66_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization protein"
                     /protein_id="HDL6320657.1"
                     /translation="MNRQGVYINGKQVFDSKGKEINSIEIIGSTVQTVVGINKGSISM
                     VSSNNALIVDGQTIAFEPSPTIHIEVFGNITNLNSGAGNVNIKGDVSHIETVSGDVNV
                     DGTVTSDVRTVSGDIRCKTIAGKVETISGDIYR"
     gene            1869..>2077
                     /locus_tag="PXD66_002417"
     CDS             1869..>2077
                     /locus_tag="PXD66_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320658.1"
                     /translation="MKDILLDFDDTSCYLGDWEEILEEYNDRVRVEDFWHYAKEFETV
                     PHLGNLYQEHVILKLIARFCIELDI"
BASE COUNT          686 a          360 c          437 g          594 t
ORIGIN      
        1 agcattccga aagggaactc cacaatattg tgggaatgcc gattaaacct ctaccatttc
       61 ggtgaattta catcgaaatt tcatttaact tccaccttgc ggggaacatt tttcccgtgt
      121 tgggtaagga tgtttctccg cttttttact ttggagaaac agactatgac aactcagact
      181 tctactcaaa aaaattactt caatcttcac acaaccggaa tcggctatct gagcaatatc
      241 cgcacagtga ctccgaaaaa aggtgatgaa ttcttagctt gtactattgg tgctctcatt
      301 ggagaagcag gagaaggtgc aaaagcagaa tatcgctatt ttgattgcaa agttgtgggc
      361 gaagctgcga ttgatttggt caatcgggca aaagcctctg ttgaagctga taagaaagta
      421 ctcatctctt tcgttttagc tgacctttgg acagatactt tcacctatca aaaagatgga
      481 aagaatcaca aaaaaggtga tactggtgta atgctgaaag ctcgcttaat tcggattaag
      541 tctgtcaaaa ttgatggagt ggtaaaatac caagaaccag aaaaaataga cgcacaatct
      601 gagtaatttt acctaagccc gacattttat gttgggcttt actttatgtc ccgtagggaa
      661 atgctccctg cggtgtgttt cccttcattt ttacaatttt aggagaaaca ctatgactat
      721 taacacttat gcaaaatttg cacctactgt tttcgtagca aaatgccctg aagcacatac
      781 caaaggtgaa attatcactc taaccagcaa atatggtaat gaaaccgaag ttcaaataca
      841 taacttagtg aagcaggata atgagcatta ttactattct ttcactcgtt gtgatgggat
      901 ggatagtcag tcatttgctc aacaaaaagc agaacgctat caaggctatg cagataatgc
      961 aaccaaacgc agccaaagct gggcaaatgc agctaatgag gggctggagt ttttgagact
     1021 aggtgagcca ataaaaattg ggcatcatag tgaaaaacgc caccgtaacc tcattgaacg
     1081 taatgcccgt cgaatggata atgcgctttc agaaatgaac aaaacagaaa gctatgaatc
     1141 ccgtattgca tattgggaaa aaatggcagg gaaaattgac ctttctatgc cggaaagtct
     1201 ggaatacttt aagtttgaat tggataaagc caaagaaaag cataaagaat tgaaggacaa
     1261 ccctgagaaa cgagagcatg gttattcact gacttatgct aaaaaagcag ttaatgaact
     1321 tgagaaaaaa gtgaaattag ctgaattact ttgggcataa tttttacata gaggattatt
     1381 aatatgaatc gacaaggcgt ttatatcaat ggtaaacaag tctttgactc taaaggcaaa
     1441 gagattaata gcattgaaat cattggtagc acagtgcaaa cagtcgttgg gataaataaa
     1501 ggatctatct caatggtctc cagcaacaat gcattaattg ttgatgggca aacaattgca
     1561 tttgaacctt ctccgactat ccatattgaa gtatttggta atattaccaa tctcaattca
     1621 ggagcaggaa atgtgaatat caaaggtgat gtaagccata tagagactgt gagtggtgat
     1681 gtgaatgttg atggtactgt gacttcagat gtaagaacag taagcggtga tattcgctgt
     1741 aaaactattg caggtaaagt cgaaaccatc agcggagata tttatcgcta atttttgcta
     1801 cttaccctat cgggaaacat tcccgatagg tgtgtttccc cacttttatt taaattttgg
     1861 agaaacatat gaaagacatt cttttggatt ttgatgacac ctcttgttat ttaggtgatt
     1921 gggaagagat actagaagaa tacaatgatc gtgttcgagt tgaggatttt tggcattacg
     1981 ctaaagaatt tgaaaccgta cctcatttgg gcaacctata ccaggagcat gtcatcctga
     2041 agttgattgc tcgattctgt attgaactgg atattga
//
LOCUS       DANLXF010000291         2064 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.292, whole genome shotgun sequence.
ACCESSION   DANLXF010000291 DANLXF010000000
VERSION     DANLXF010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.292"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..726
                     /locus_tag="PXD66_002418"
     CDS             <1..726
                     /locus_tag="PXD66_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005578999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL6320659.1"
                     /translation="VKVVRNNPNVGAAQSRNVGATNATQEWLLFLDDDDRFMPEKCER
                     LAAEIANNPTANFVYHPAKCEMVNEGFTYVTKPFKHKSELTKENILKANKIGGMPMIG
                     LKKSLFCKIGGLSDRLRSLEDYDFLLKLLEEPSFEPLYVDEALTYCTFHTKRSSVSTD
                     TTNTEKAIEYIQQTYIKTPEANNFKLNSLYILSYPHVMNLSRKAAGYYFEMFKQTKNI
                     KQLIIALVIFISPKLAINLKRFM"
     gene            726..1526
                     /locus_tag="PXD66_002419"
     CDS             726..1526
                     /locus_tag="PXD66_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_162680708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL6320660.1"
                     /translation="MKFSVLMSLYIKEQPQYLRECFESLKVQTVQADEIVVCFDGAVT
                     PELEAIVEEYSAILPINAVKFPQNRGLGKTLNDGLTYCKNEWVFRMDTDDICMPERFE
                     KQVAFIEQNPETVIFGGQIAEFGQDIHDLVAYRNVPTTVEEIVRFTRLRCPFNHMTVA
                     YQKSKVLEVGGYQDLQEDYYLWIKLIAQFKQVANLPDLLVYARVGNGMVGRRRGMAQA
                     EAEWRLYKLKNRCQIHDPISGFGIFIMRAIPRLLPVSALKKVYGLLRK"
     gene            complement(1563..2060)
                     /gene="msrA"
                     /locus_tag="PXD66_002420"
     CDS             complement(1563..2060)
                     /gene="msrA"
                     /locus_tag="PXD66_002420"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007525436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL6320661.1"
                     /translation="MREIYLAGGCFWGVESFMQRIKGVIKAQSGYANGNTDNPSYQEV
                     CAGSGHAEVVKVTYDANQISLAKLLDYFFKVIDPVSINKQGGDIGIQYRTGIYYTDPQ
                     DEPVVTAALAQLQTQFDQPLAVENQMLEQYFPAEEYHQEYLVKNPNGYCHIDIQLMNE
                     ILKNQ"
BASE COUNT          618 a          376 c          445 g          625 t
ORIGIN      
        1 gtgaaagtgg tgcgaaataa cccaaatgtt ggggcggcac aaagccgaaa tgtaggggca
       61 actaatgcca cacaagaatg gctactgttt ttagatgatg atgaccgctt tatgcctgaa
      121 aaatgcgaac gtttagcggc agagattgcg aacaatccaa ctgccaattt tgtgtaccac
      181 ccggcaaaat gtgaaatggt aaatgagggt tttacttatg tcaccaagcc gtttaaacat
      241 aaaagtgagc taactaaaga aaacattcta aaagcgaata aaattggcgg aatgccgatg
      301 attggcttga aaaaatcact cttttgtaaa attggtggat tatctgaccg cttgcgatca
      361 cttgaagatt atgatttctt actgaaatta ttggaagaac cgagttttga gccattgtat
      421 gtggacgaag ccttgaccta ttgtactttc cataccaaac gctccagtgt ttcaaccgat
      481 accactaata cagaaaaggc tattgagtat attcaacaaa cgtatatcaa aacacctgag
      541 gcgaataatt ttaagctcaa ttcgctttat attttaagtt atccacacgt aatgaattta
      601 tcccgcaagg cagccggtta ttattttgag atgtttaaac agactaaaaa tatcaaacaa
      661 ctgattattg cgttggttat ttttatttcg ccgaagttgg cgattaattt gaaaaggttt
      721 atgtaatgaa attttccgtt ttaatgtcgt tatacattaa agagcaaccg caatatttaa
      781 gagaatgttt tgaaagcttg aaagtacaaa ctgtgcaggc tgatgaaatc gttgtttgtt
      841 ttgatggtgc agttacgcct gaattagagg cgattgtaga agaatatagt gcaattttgc
      901 cgattaatgc ggtgaaattc ccacaaaatc gaggtttagg taaaacgcta aatgatgggc
      961 taacctattg taagaatgaa tgggttttca gaatggatac agacgatatt tgtatgccgg
     1021 aacgtttcga gaaacaagtg gcatttattg aacagaatcc tgaaaccgtg atttttggcg
     1081 gccaaattgc tgaatttggg caagatattc acgatcttgt cgcttaccgc aatgtaccaa
     1141 ccacggtgga ggaaatcgtc agattcactc gcttgcgttg cccgtttaac catatgacgg
     1201 ttgcttatca gaaatccaaa gtgttggaag tcggtggcta tcaagatctg caagaagatt
     1261 actatttatg gatcaaactc attgctcaat ttaaacaagt ggcaaacttg ccggatttat
     1321 tagtgtatgc ccgagtcggt aacggaatgg ttggcagacg ccgtggaatg gcccaagcag
     1381 aggcagaatg gcgtttatat aaattaaaaa accgctgcca gattcacgat ccgatttcag
     1441 gttttgggat tttcataatg cgtgcaattc ctcgtttatt accggtttca gcactgaaaa
     1501 aagtgtacgg tttgctcaga aaataaagct aaaaaaacca cagattgctc tgtggttttt
     1561 tgttattgat tcttcaaaat ctcattcatt aattgaatat caatatggca gtagccgttc
     1621 gggtttttta ctaaatattc ttggtggtac tcttcggcag ggaaatattg ctctaacatt
     1681 tggttttcca ctgccagcgg ctgatcaaat tgggtttgta attgtgcaag tgcggcggtt
     1741 accaccggtt cgtcttgtgg gtcggtataa taaataccgg tgcggtattg aataccgatg
     1801 tcgccacctt gtttgttaat gctcaccggg tctattactt taaagaagta atctagtaat
     1861 tttgccaagc taatttgatt ggcatcataa gtcactttta ccacttcagc gtgtccgcta
     1921 ccggcacaca cctcttgata gcttgggtta tcggtattac cgttggcata gcctgattgt
     1981 gccttaatga cacctttaat ccgttgcata aaagattcca cgccccagaa acagccaccg
     2041 gctaagtaaa tttcacgaat attt
//
LOCUS       DANLXF010000292         2041 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.293, whole genome shotgun sequence.
ACCESSION   DANLXF010000292 DANLXF010000000
VERSION     DANLXF010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.293"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(51..1934)
                     /gene="htpG"
                     /locus_tag="PXD66_002421"
     CDS             complement(51..1934)
                     /gene="htpG"
                     /locus_tag="PXD66_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HDL6320662.1"
                     /translation="MANNQETRGFQSEVKQLLQLMIHSLYSNKEIFLRELISNASDAA
                     DKLRFKALSEPALYEGDGELRVRVSVDETLGTITIADNGIGMTREQVIDHLGTIAKSG
                     TKEFLNALGTDQAKDSQLIGQFGVGFYSSFIVADKVTVKTRAAGVPAEQGVIWESAGE
                     GDYTVADLEKKERGTEIILHLREDEKEFLNEWRLREIIGKYSDHIGLAVEIQTNEYDD
                     EGKASGQKWEKINKAQALWTRSKNEISDEEYTEFYKHLSHDFADPLIWSHNKVEGKQE
                     YTSLLYVPSKAPWDLFQREQKHGLKLYVQRVFIMDDAEVFMPNYLRFMRGLLDTNDLP
                     LNVSREILQENKVTQSLRSALTKRALQLLEKLAKDDAEKYAQFWQAFGVVLKEGVGED
                     FANKQQVAGLLRFASTQRDDSEQAVSFADYIARMKEGQKAIYFLTADSYQAAKNSPHL
                     ELFHKKGIEVLLLSDRIDEWLVSHLTEFDGKPLQSITKSDLDLGELADKQEEESQKAQ
                     QAEFGSFLERAKGYFGERVKNVVLTHRLTDTPAVVSTDSDEMTTQMAKLFAAMGQQAP
                     EVKYTFELNPDHPMVKKVADLADEAEFADWIELLFEQALLAERGTLENPTAFIKRVNK
                     LLG"
BASE COUNT          478 a          497 c          472 g          594 t
ORIGIN      
        1 tatattgaat atggaaaaac ggaaacctag atttccgttt ttagtaatgg ctagcccaac
       61 aatttattta cccgtttaat aaaggctgtc gggttttcca acgtgcctcg ttcagcaagc
      121 agagcttgtt caaacagcag ttcaatccaa tcggcgaact cggcttcatc ggcaagatca
      181 gctacttttt tcaccattgg gtgatctggg ttcagctcaa aagtatattt cacttctggt
      241 gcttgttgcc ccattgctgc aaacagtttt gccatttggg tggtcatttc atcgctgtca
      301 gtggaaacca ctgccggtgt gtcggttaag cggtgagtca gcaccacgtt tttgacccgt
      361 tcaccaaagt agcctttcgc ccgttctaag aacgaaccaa actccgcctg ttgggctttt
      421 tggctctctt cttcctgctt gtcggcaagc tcgcctaagt ctaagtccga cttggtaatg
      481 ctttgtagcg gtttgccgtc aaactcggtg aggtggctaa ccaaccattc atcaatgcgg
      541 tcagaaagca gtagcacctc aatgcctttt ttgtggaaca gctctaagtg cgggctgttt
      601 ttagccgctt ggtagctgtc tgcggttaag aagtagatcg ctttctgccc ctctttcatt
      661 cttgcaatat aatcggcaaa actgaccgct tgttcgctgt catcacgctg ggtggaggca
      721 aaacggagta agcctgcaac ctgttgtttg ttggcaaagt cttcgcccac gccctcttta
      781 agcaccacgc cgaacgcttg ccagaattgg gcatattttt cggcatcgtc tttcgccaat
      841 ttttccagca gttgtaacgc acgcttggtt aaggcagaac ggagcgattg ggtaacttta
      901 ttctcttgca gaatttcacg gctgacgttg agcggcaggt cgttggtgtc gagcaagcca
      961 cgcataaaac gcaagtagtt cggcataaag acttccgcat cgtccataat aaatacacgc
     1021 tgaacgtaga gtttcaagcc gtgtttttgc tcgcgttgga acagatccca cggggctttg
     1081 ctcggcacat acagcaagct ggtgtattct tgcttacctt cgactttatt gtgcgaccaa
     1141 atcagcgggt cggcaaaatc atggctgagg tgtttgtaaa attcggtgta ctcctcgtca
     1201 ctaatctcat tttttgagcg tgtccaaagc gcttgagctt tgttaatttt ttcccatttt
     1261 tgaccgcttg ctttgccctc gtcatcgtat tcattggtct gaatttccac cgccaggccg
     1321 atatggtcgg agtatttgcc gataatctca cgcaaacgcc attcgtttaa aaactctttt
     1381 tcgtcctcac gcaagtgaag aatgatttcc gtgccacgct cttttttctc cagatcggca
     1441 acggtgtaat cgccctcacc ggcagattcc caaatcacgc cttgctcggc aggcactccg
     1501 gcggcacggg ttttgactgt cactttatcc gccacgatga aagaagagta aaagcccacg
     1561 ccgaactgcc cgataagctg gctgtctttg gcttgatcag tgccgagtgc gttcaaaaat
     1621 tctttggtac cagattttgc aatcgtaccg agatggtcga tcacctgctc acgggtcata
     1681 ccgataccgt tatcggcaat cgtaatcgta ccgagtgttt catcaacaga gacacgcaca
     1741 cgcagttcgc catcaccttc atacaaagcc ggctcggaaa gggctttaaa ccgcaattta
     1801 tcggcggcgt cagaggcgtt ggaaataagc tcacgcagga aaatttcttt gttggagtaa
     1861 agcgagtgga tcatcagttg caataactgt ttcacttccg actggaagcc acgggtttct
     1921 tgattatttg ccattgtgta ttcctttatt cattaaaaaa cagggaatag atggggcaag
     1981 tggtcagatt ttcaagattt tttgcaaaat atctgttgcc aaatagcgaa ttaattgaaa
     2041 g
//
LOCUS       DANLXF010000293         2025 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.294, whole genome shotgun sequence.
ACCESSION   DANLXF010000293 DANLXF010000000
VERSION     DANLXF010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.294"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            248..946
                     /gene="aphA"
                     /locus_tag="PXD66_002422"
     CDS             248..946
                     /gene="aphA"
                     /locus_tag="PXD66_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HDL6320663.1"
                     /translation="MTAATMFASLIAFSAQAGGPKVAYTQPGIDMRTHEAQQAPIHWV
                     SIEQIKESIKDLPPMAVSFDIDDTVAATSGCFHYGKQKYSPDDFSYLKNQDFWDEINA
                     GCDKYSLPKQVAINLIKMHQERGDQVYLITGRTAGKNEQVTTIMQKYLGIKNMQPVNF
                     MGGKAHSTKYDKTPAIIEHKVQIHYGDSDDDILAAREAGIRGIRILRAANSNYTPFPQ
                     AGGYGEEVVVNSSY"
     gene            1127..1888
                     /gene="udp"
                     /locus_tag="PXD66_002423"
     CDS             1127..1888
                     /gene="udp"
                     /locus_tag="PXD66_002423"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HDL6320664.1"
                     /translation="MSEVFHLGLTKAMLKGAKIAIVPGDPARSERIAKKMDNPEFLAS
                     TREFTSWLGYLDGEPVVVCSTGIGGPSVSICVEELAQLGVRTFLRIGTTGAIQEHINV
                     GDILITTGAVRLDGASQHFAPMEYPAVANFECTNALYAAAVESGVTPYVGITASSDTF
                     YPGQERYDTFTGKIYRHFQGSLKQWQDLNVMNFEMESATLFTMCSALGLRAGMVAGVI
                     VNRTQQEIPNEATIHDTEHKAVSVVVEAARKLIKG"
BASE COUNT          637 a          353 c          429 g          606 t
ORIGIN      
        1 tattaagcga atatgatgcc tatcaattta cgaatgaggt gattaaattt cattcagaag
       61 aaagccttaa agcttgggga ggttgcttga tttgggaaca gaacaatggc tcataagcgt
      121 taagcgtaat cgtttgcttg tgatcatatt cacaaaataa ggtttttgta ttagctaaga
      181 gtgcctaata agattaaaat aggtatggtt aatcatatgg aggtatttta taaaatttac
      241 attaaaaatg acggcagcga caatgtttgc atctcttatt gctttttcag cacaagcagg
      301 cggaccaaaa gtagcttata cccagccggg aattgatatg cgaacacatg aagcacagca
      361 agcgccaatt cattgggtat ctattgagca aattaaagaa agcatcaaag atttaccccc
      421 tatggcggta agttttgata ttgatgatac tgttgctgca actagcggtt gcttccacta
      481 cggaaagcaa aaatattcgc cagacgattt cagctactta aaaaatcaag atttttggga
      541 cgaaattaat gctggctgtg ataaatattc tttgccaaag caagttgcta ttaatttaat
      601 aaaaatgcac caagaacgtg gtgatcaagt ttatttaatt acggggcgta ccgcaggtaa
      661 aaatgaacaa gttacaacga ttatgcaaaa gtatcttggt attaaaaata tgcaaccggt
      721 aaattttatg ggggggaaag cacattcaac aaaatatgat aaaacccctg cgattattga
      781 acataaggtg caaattcact atggtgacag tgatgatgat atcttagctg cccgtgaagc
      841 cggtattcga ggtattcgta tacttcgtgc agcaaattct aattatacgc cattccctca
      901 ggccggagga tatggtgaag aagttgttgt caattcaagt tattaatttc ttagtctgaa
      961 atatggccac cattgcgtgg ccatcatttt ttatttatat aatgacgaaa ccgtttacct
     1021 ttgatctagt tcacaaaatc aaactttgct tgcaaaaaat tgtcgtaaat taaccgcttg
     1081 ctaagataga atggctcata tccagttaga taaaaaggag ataaaaatgt ctgaagtatt
     1141 tcatttaggt ttaaccaaag ccatgctcaa aggtgcaaaa attgcaatcg ttccgggcga
     1201 tccggctaga agtgaacgta ttgccaaaaa aatggacaac ccagagtttt tagcctcaac
     1261 ccgtgaattt acctcttggt taggttattt agatggcgaa ccggtggtgg tttgttcaac
     1321 cggtattggc ggaccgtcgg tgtcgatttg tgtggaagag ttagcacagt taggtgttcg
     1381 cacattctta cgcattggca caacaggtgc aattcaagag catattaatg taggcgatat
     1441 tttaattacc accggagcgg tgcgtttaga tggtgcgagc caacattttg caccaatgga
     1501 atatcctgca gtagcgaatt ttgaatgtac taatgctctt tatgcagcag cagtggaatc
     1561 aggcgttaca ccttatgtgg ggattacagc ttcatcagac actttctacc cggggcaaga
     1621 acgttacgac actttcaccg gcaagatcta tcgtcatttc caaggttcat taaaacagtg
     1681 gcaagatctc aatgttatga attttgaaat ggaatccgcc actctcttta cgatgtgttc
     1741 ggcattaggc ttgcgagcag gtatggttgc tggtgtaatt gtaaaccgca ctcaacaaga
     1801 gattccgaat gaagcaacca ttcacgacac cgaacataaa gccgtttcgg tagtagtgga
     1861 agcggcaaga aaattaatta aaggctaatc tttaaattta aaagcaaaaa aggtaaattg
     1921 tctttgttac aatttacctt ttttcttttt agggagcttt ctatgaaaaa atggttgatg
     1981 ttaattacat taattttcgg cattaccgct tgtttttcca gttta
//
LOCUS       DANLXF010000294         2010 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.295, whole genome shotgun sequence.
ACCESSION   DANLXF010000294 DANLXF010000000
VERSION     DANLXF010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.295"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..810)
                     /locus_tag="PXD66_002424"
     CDS             complement(<1..810)
                     /locus_tag="PXD66_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320665.1"
                     /translation="MSDSVLNTYFKHALVKAGFPDTLDMEYSLSHCQGDGVAFYGRLG
                     TDDLIQLFNHLNPQKRKQKMFANLLTHIESWETHDDIGFEIIRNRFGYRYSHWNTMEL
                     YAKTADQLVFFNDSDARRNWYFPTSKVDKYKALWDGFIEDLEQYIKDISREMESVGYQ
                     IIEASPYQTETVFKFNTEKYLVELQRKPSEFYSELVDWAFGDMCDFEAAIEQICQGQL
                     CYADFEATVTDKKSEIQLGLARLSGISYSPNDRSFSGYRQELIGEAIASARH"
     gene            complement(1074..1316)
                     /locus_tag="PXD66_002425"
     CDS             complement(1074..1316)
                     /locus_tag="PXD66_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320666.1"
                     /translation="MFGKLFYKMNPKVLAKIGEDFRKAGYLMGAGFVGMVISNDQIST
                     SEGFVLFLWGLYIWIIGHIALHHSEKILNSKQGEPK"
     gene            complement(1325..1570)
                     /locus_tag="PXD66_002426"
     CDS             complement(1325..1570)
                     /locus_tag="PXD66_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
                     /protein_id="HDL6320667.1"
                     /translation="MSVHIKLSCVGDILPIKLNEWTPEHRALHHEISHKALEAWQNRD
                     KGTQSVRFMQQMPARQGLHFRFLSVSEQDGKLIVSRS"
     gene            complement(1642..1995)
                     /locus_tag="PXD66_002427"
     CDS             complement(1642..1995)
                     /locus_tag="PXD66_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320668.1"
                     /translation="MSQQTAGWDVIPFESSKTAKPSLFWKLGNKLGNIFVSAKHKAKS
                     KYVEVTKGINQRIAEEDEMRALELQMCLDEQAAEYEQEISRIKRKMFFRALFVAIIAF
                     LCGMATVFGTQLSLI"
BASE COUNT          628 a          424 c          365 g          593 t
ORIGIN      
        1 atggcgagca gaggcaatcg cctcaccaat aagctcttgt cgataaccac taaacgaacg
       61 gtcattcggc gagtaactta ttccactcaa gcgagcaaga cctaactgaa tttcactttt
      121 cttatcggta acagttgctt caaaatcagc ataacagagt tgcccttgac aaatctgctc
      181 tatagctgct tcaaaatcac acatatcacc aaatgcccaa tcaacaagct ctgaataaaa
      241 ctcagaaggt ttacgttgca gttctactag atatttttcc gtattaaatt tgaaaacggt
      301 ttccgtttga tacggcgagg cttcaataat ttgataaccg accgattcca tttcacggga
      361 aatatctttg atgtactgct ccaaatcttc gataaatccg tcccacaatg ctttgtattt
      421 atccactttt gaagtaggaa aataccagtt tcttcgagca tcactatcat tgaaaaacac
      481 taactgatct gcagttttag cataaagctc catcgtattc caatgcgagt aacgataccc
      541 aaagcgattt cgtataattt caaaaccaat atcatcgtgg gtttcccaag attcaatgtg
      601 cgttagcaaa ttagcaaaca ttttctgttt acgtttctgt ggattgagat gattgaaaag
      661 ctggattaga tcatctgtac caagccgacc ataaaatgcg actccatctc cttgacaatg
      721 gctgagtgaa tactccatat ctaaggtatc cggaaaacca gctttgacaa gagcgtgttt
      781 aaaataagtg tttaatactg aatctgacat aatgtttctc caaaataaaa aataatggga
      841 aacacttaag cccaattcgg gaaagtattt cccgataggg taaaaggaag gtaaaacgga
      901 gaacaagctc ctcggggaac ttgttcccct atgggttaga atggagtttt gctatttaga
      961 ctgtggctta tctgattttg taaacaaaat atctcgattt gcccaagtta ctagaacaat
     1021 aaaaattgtt gccaataacg ctgttccaat agcagcacct aaaaagtacg gactcatttc
     1081 ggttctcctt gctttgaatt aagtattttc tcagaatgat gtagtgcgat atgcccgata
     1141 atccaaatat acaatcccca caaaaaaaga acaaatcctt ccgatgtgga aatctgatca
     1201 ttggaaatta ccattccgac aaaaccagct cccataagat aaccagcttt tctaaaatct
     1261 tctccgattt tagctagtac tttgggattc attttgtaaa aaagcttacc aaacattgaa
     1321 ccaactatga acgactgaca atgagcttgc cgtcttgctc cgatacactc agaaagcgga
     1381 aatgcagtcc ttgtcgggct ggcatttgtt gcataaatcg cacgctttga gtgcctttat
     1441 ctcgattttg ccaggcttcg agagctttat ggctaatttc gtgatgtaag gctcgatgtt
     1501 ccggtgtcca ttcattgagt ttgattggca aaatatctcc aacacaggag agtttgatat
     1561 gaactgacat acatttctcc aaaattaagg ggaaatgtat taccacaagg gaaatagcat
     1621 ttccccgatg ggtgagttaa attagattaa agagagctga gtaccaaaaa cggtagccat
     1681 accacataaa aaggcaataa tcgcaacgaa taaagccctg aaaaacatct tacgcttaat
     1741 gcgagaaatt tcctgctcat attcagctgc ttgctcatct aagcacattt gcaattctaa
     1801 ggctcgcatt tcatcttctt cagcaattcg ttgattgatg cctttggtta cctcgacata
     1861 tttgctttta gctttatgtt tcgccgaaac gaaaatatta ccgagcttat tgccaagttt
     1921 ccaaaaaaga gaaggttttg cggtttttga cgattcaaat gggatcacat cccatcctgc
     1981 ggtttgttga ctcatagcga gttctcctat
//
LOCUS       DANLXF010000295         2001 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.296, whole genome shotgun sequence.
ACCESSION   DANLXF010000295 DANLXF010000000
VERSION     DANLXF010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.296"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            95..775
                     /locus_tag="PXD66_002428"
     CDS             95..775
                     /locus_tag="PXD66_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00153 family protein"
                     /protein_id="HDL6320669.1"
                     /translation="MALNNILGLFAQSPLKPLQKHSNKVTECSELLESFFEATFEHDW
                     EKAAEVRGKIVDLERRADALKREIRLKLPRGLFMPVERGDLLELVTQLDKLANYSRDI
                     SGRIIGRKLIIPTEMQPLFKKFLSRSIDASRQVRKVLDEMDQLLETGFRGRELDFVNK
                     MILELDQIEDDTDQYQITLRHTLLELEKNLNPIDVMFLYKCIERISVLADQAQRIGSR
                     IELMLAKS"
     gene            787..>2001
                     /locus_tag="PXD66_002429"
     CDS             787..>2001
                     /locus_tag="PXD66_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HDL6320670.1"
                     /translation="MELLNQYGSILVIITAIVGFMMAFGIGANDVSNAMGTSVGSGTI
                     TIKQAILIAMVFEFAGAYLAGGEVADTIKSGIIDPAQFANSPDILVLGMMASLCAAGM
                     WLIIATKMGWPVSATHTIIGAVIGFALITIGSDAIQWDALGGIVGSWFVTPILAGIVA
                     YSIFINSQKLIFNRSNPFKQAKKYGPMYMGITIFILVIVTVSKGLKHVGLHLDTAETL
                     GISFALALLAFIIGYFYFRSKSFAMKARAEEKGFAGVEKIFSILMLITACAMAFAHGS
                     NDVANAIGPLAAVESIVRSGGMIEGKTTLAPWILPLGALGMMIGLAIMGKNVMATVGT
                     GITDLTPSRGFAAQFACAVTVVIASGTGLPISTTQTLVGAVLGVGFARGIAALNLGVI
                     RNIVISWVITLPV"
BASE COUNT          567 a          359 c          427 g          648 t
ORIGIN      
        1 tttaatttcg tgatctttac taatacatca agtaaaatac gccggacttt acagtaagaa
       61 gtcattgtca atattatcac tatggagata ttttatggca ttgaataaca ttttaggttt
      121 attcgcccag tccccactta aaccgttaca aaaacactca aataaagtga ctgaatgtag
      181 cgagttatta gaatcttttt ttgaagcgac ttttgaacat gattgggaaa aagcagctga
      241 agtgcgcggt aaaattgttg atttagaacg ccgcgcagat gcccttaaac gtgaaatccg
      301 cttaaaactg cctcgtggtt tatttatgcc agttgaacgc ggtgatttat tagaactagt
      361 cacacaatta gataagttag caaactattc aagagatatt tccggtcgca ttattggtcg
      421 taaattaatc attcctaccg aaatgcaacc acttttcaaa aaattccttt ctcgtagtat
      481 tgatgctagc cgtcaggtac gcaaagtttt ggatgaaatg gatcagttat tagaaactgg
      541 ttttagaggg cgagagcttg atttcgtcaa taaaatgatt cttgaacttg accaaattga
      601 agatgatacc gaccaatatc aaattacatt acgtcataca cttcttgagt tagaaaaaaa
      661 cctgaaccct attgatgtga tgtttttata caaatgtatt gaaagaatca gcgttctagc
      721 tgaccaagcc cagcgaattg gttctcgtat tgaacttatg ctggcgaagt cataggagaa
      781 aattaaatgg aattactcaa tcaatatggc tcaatactgg tgattattac tgctattgta
      841 gggtttatga tggctttcgg tattggtgct aatgatgttt caaatgccat gggaacatcc
      901 gtcggctctg gaacaattac gattaaacaa gctattttaa ttgctatggt atttgaattt
      961 gctggtgctt atctggcagg cggtgaagtc gcagatacaa ttaaaagtgg cattattgat
     1021 ccggcacaat tcgccaatag ccctgatatc ctggtacttg gaatgatggc atctctctgt
     1081 gctgccggaa tgtggctaat tattgcaact aaaatgggtt ggccggtttc agcaactcac
     1141 acaatcattg gtgccgttat tggttttgca ttaatcacta ttggttcaga tgcaattcaa
     1201 tgggatgcat taggtggaat tgttgggagt tggtttgtga cccctattct tgcggggatc
     1261 gtagcatatt cgatctttat taactcacaa aaactgatct ttaaccgctc aaacccattc
     1321 aaacaagcga aaaagtatgg cccgatgtac atgggaatta ctatttttat tttagttatt
     1381 gtaaccgtgt caaaaggctt aaagcacgta ggattacatc tagatacagc tgaaacattg
     1441 ggaatttcat tcgcgcttgc cttacttgct tttatcattg gatattttta tttccgcagt
     1501 aaaagctttg caatgaaagc tcgtgcagaa gagaaaggct tcgctggcgt tgagaaaatt
     1561 tttagtattt taatgcttat tacggcctgt gctatggctt ttgctcatgg ctctaatgat
     1621 gttgctaatg ctattgggcc tctagctgct gttgaatcta ttgtaagaag tggtggaatg
     1681 attgaaggta aaacgacttt agccccttgg attctcccac taggcgcctt aggtatgatg
     1741 attggtttag ctattatggg caagaatgta atggctacag taggtactgg tattaccgac
     1801 cttactccaa gccgtggctt tgctgctcaa tttgcctgcg ccgtcacggt tgtcattgca
     1861 tcaggtactg gtttaccaat atcaacaacc caaacgcttg taggagcagt attaggtgtg
     1921 ggattcgccc gtggtattgc tgcacttaac ttaggcgtta ttcgtaatat tgttatatca
     1981 tgggtaatta ccttgcctgt a
//
LOCUS       DANLXF010000296         1961 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.297, whole genome shotgun sequence.
ACCESSION   DANLXF010000296 DANLXF010000000
VERSION     DANLXF010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.297"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..120
                     /locus_tag="PXD66_002430"
     CDS             <1..120
                     /locus_tag="PXD66_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HDL6320671.1"
                     /translation="SNLDEKQMMWQVVIHLAFIVSALAMAYIDKMLNSGHMKH"
     gene            197..1567
                     /gene="gorA"
                     /locus_tag="PXD66_002431"
     CDS             197..1567
                     /gene="gorA"
                     /locus_tag="PXD66_002431"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDL6320672.1"
                     /translation="MAKHYDYIAIGGGSGGIASINRAASYGQKCAIIEAKHLGGTCVN
                     VGCVPKKVMFYGAQVAEAINHYAPDYGFDITVNNFDYAKLVESRQAYIGRIHTSYGNV
                     LAKNNVDVLNGFARFKDAKTLEVSYADGSVEEVTADHILIATGGRPTVPNVKGAEYGI
                     TSDGVFALDALPKSVAIVGAGYIAVELAGVFNSLGVNTHLFVRQHAPMRNQDPLIVDT
                     LLEVLAQDGIELHTQATPQEVVKNADGSLTLKLQDGRETSVESLVWAIGREPATDKIN
                     LEAAGVETNERGFIKVDKFQNTNVPNIYAVGDIIEGGIELTPVAVAAGRRLSERLFNN
                     KPNEHLDYSLVPTVVFSHPPIGTVGLSEPKAIEQYGAENVKVYKSSFTAMYTAVTQHR
                     QPCRMKLVCVGEGEKVVGLHGIGFGVDEMIQGFAVAIKMGATKADFDNTVAIHPTGSE
                     EFVTMR"
     gene            1578..>1961
                     /locus_tag="PXD66_002432"
     CDS             1578..>1961
                     /locus_tag="PXD66_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320673.1"
                     /translation="MLRNFMIMLHFIYGFAMKNAFSFFVISLFLTMNSISYASNPIPS
                     NKEKLNGVKKQQDKPKFRISERKVLKNKEKDEEVSNFSMEELRVGAQRPLDDSVIQYF
                     KSQCRYAFMSDREIIANRCEVKKIAD"
BASE COUNT          607 a          288 c          446 g          620 t
ORIGIN      
        1 tcaaatcttg atgaaaagca aatgatgtgg caagtagtta ttcacttagc ctttattgtt
       61 tcagcattag cgatggcgta tattgataaa atgttgaatt cagggcatat gaaacattag
      121 tcttctatgc aaatttaatt tgataaagcg cttaacgaat aagttgcttt tgtttttata
      181 gatcgaggaa tagcttatgg ctaaacatta tgattatatt gctatcggcg gtggtagtgg
      241 tggtattgct tcaattaatc gagcagcaag ctatgggcaa aaatgtgcca ttattgaagc
      301 aaaacattta ggtggcactt gtgtgaacgt gggatgtgta cccaaaaaag tgatgttcta
      361 tggtgcacaa gttgcagaag cgattaacca ttacgcccct gattatggtt ttgacattac
      421 ggtaaataat tttgattatg ccaaattagt tgagagtcgc caggcctata ttgggcgcat
      481 tcatacctca tatggtaatg tgctggcaaa aaataatgtt gatgttttaa atggttttgc
      541 tcgctttaaa gatgctaaaa cattagaagt gagttatgct gatggttccg ttgaggaggt
      601 cacagcagac catattctga ttgcaacagg tggtcgtcca actgtaccca atgtgaaagg
      661 ggctgaatac ggtatcactt ctgatggtgt atttgcacta gatgctttac cgaagtccgt
      721 agcaattgtg ggggcagggt atattgcagt tgaattagct ggagtattta atagtttagg
      781 agtaaatacc catctttttg ttcgtcaaca tgctccaatg cgtaatcaag acccattaat
      841 agtggataca ttgcttgaag tattagctca agatgggatc gaattacata cacaagcgac
      901 tcctcaagaa gtagttaaaa atgcagatgg ttcattgaca ttaaaattgc aagatggtcg
      961 tgaaacctct gttgaaagct tggtgtgggc tattggtcgt gagccagcga cagataagat
     1021 taatttagag gctgctggtg ttgaaaccaa cgagcgtggt tttatcaaag tggataaatt
     1081 ccaaaatact aatgtgccaa atatctatgc tgttggtgat attattgaag gtggtattga
     1141 attaactcct gttgcagttg cagctggtcg caggctatcg gaacggttgt tcaataataa
     1201 accaaatgaa catttggatt atagcttagt accgacagtc gtttttagcc acccaccaat
     1261 aggtactgtt ggtttaagtg agccaaaagc gattgagcaa tatggggcag aaaatgtgaa
     1321 agtgtataaa tcttcattta ctgcaatgta tactgcggtt acacaacatc gtcaaccttg
     1381 ccgaatgaaa ttagtttgtg ttggggaagg ggaaaaagtg gttgggttac atggtattgg
     1441 ttttggtgtt gatgaaatga ttcaaggttt tgctgttgca attaaaatgg gcgcaacgaa
     1501 agctgatttt gacaacacag tggctattca cccgacagga tctgaagaat ttgtaaccat
     1561 gcgctaaatg taataaaatg ttacgtaatt ttatgattat gctacatttt atttatgggt
     1621 ttgctatgaa aaacgctttt agcttttttg ttatttctct ttttcttacg atgaatagta
     1681 ttagttatgc aagtaatcct attcctagta ataaagaaaa attaaacgga gtaaaaaaac
     1741 agcaagacaa gcctaagttt agaatatctg aacgtaaggt attgaaaaat aaagagaaag
     1801 atgaagaggt tagtaatttt tctatggagg aattacgtgt tggagcacaa cgacctcttg
     1861 atgattctgt gattcaatat tttaaatctc aatgccgata cgcatttatg tcagatcggg
     1921 agattattgc taaccgctgt gaagttaaaa aaatagctga t
//
LOCUS       DANLXF010000297         1946 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.298, whole genome shotgun sequence.
ACCESSION   DANLXF010000297 DANLXF010000000
VERSION     DANLXF010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.298"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(63..938)
                     /locus_tag="PXD66_002433"
     CDS             complement(63..938)
                     /locus_tag="PXD66_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL6320674.1"
                     /translation="MWIWFTLLAAVAQAGRNAFQKQLSVNVPVLGVTLARFFYAVPLA
                     SGYLYFLYQYDDNLNIPHFPPLFFVYAIGAGLAQILATSLMVQLFHLKNYAIGVGLAK
                     SEAVWAALLGVLFFGVTISGIGWLGVVLGGVAIFLMTGSGNLRQLSWKTLFLGIASGL
                     CFALTSLWVREASLQLNSHYLLSAAWVLFSVISFEAVILVIYLALLQKETLIKFFRFP
                     KLGMLTSLFSFLGSFGWFNAMSLNHVAFVKTLGQVEIFFILGISYFVFKERLKIQDFI
                     GLILVVIAAILVVLG"
     gene            1046..1882
                     /locus_tag="PXD66_002434"
     CDS             1046..1882
                     /locus_tag="PXD66_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDL6320675.1"
                     /translation="MLPHIYIGTGGYADTDLIGTLYPFGTAKEDFLFVYSQHYDTLEI
                     NSTFHAPIGQKAFEGMVQKAEGRLNFSVKLHQDFSHQRTATLDQAQLFLNALAPFTAE
                     GLLANLFVQFPTQFERMPLNRRYLAELCQWFADYPLAIEFRHPSWHIPAVFDTFAKSS
                     NLIWCNVDYPQNIGLPAFHFFANQRTAYLRLHGRNPNWWKGQSAAERHDYRYSDEELK
                     GLAELLFSRRTEFDRLYLYFENTTKSHAFYNIQTLKGFLAEKGFQIKPVPENLLGQQS
                     LF"
BASE COUNT          607 a          444 c          405 g          490 t
ORIGIN      
        1 attggttttg cgtgggctta cgcccaccct acaaagctag ataaaaaacg taaaaatccg
       61 atctagccca gcaccaccaa gattgcggca atcactacta aaatcagccc tataaaatct
      121 tgaattttaa gccgttcttt aaacacaaaa taggaaatac cgagaataaa gaaaatttcc
      181 acttgtccaa gcgtttttac aaaggcaacg tggttcaggc tcattgcatt gaaccaaccg
      241 aatgagccga gaaatgaaaa caggctggtc aacataccta attttggaaa acgaaagaat
      301 ttaatcagtg tctctttttg gagaagagct aggtatataa ccaatatcac tgcctcaaag
      361 ctaatcacgc taaaaagtac ccacgccgct gaaagcaaat agtggctgtt gagttgcagg
      421 cttgcctctc gcacccaaag tgatgtgagg gcgaaacata aaccgctggc tatgccgaga
      481 aagagtgttt tccacgaaag ttggcgtaga ttaccgctgc cggtcattaa gaaaatcgcc
      541 actccaccta gtaccacgcc aagccagcca atgccactga tggttacacc aaaaaataac
      601 acgcccagta aagccgccca tactgcctcg cttttcgcca gcccgacacc tattgcataa
      661 ttttttaagt gaaaaagttg caccattagc gaagttgcca gaatttgagc caacccagcc
      721 ccgatagcat atacaaaaaa cagcggtgga aaatggggaa tgttgaggtt atcgtcatat
      781 tggtataaaa aatagagata accgcttgcc aacggcacag catagaaaaa gcgggcgagc
      841 gttaccccca atacaggcac attgacgctt aattgctttt gaaaggcatt gcgaccggct
      901 tgagccacgg ctgccagcag agtaaaccaa atccacataa tgatgatgaa ataagaaaag
      961 atagccaaat cttactcttt tattctcagt ttgcaagcgg ttaaaaaacc attaaaattt
     1021 gcaaaaaatt gaaagaataa acacaatgct ccctcatatt tatattggca ccggcggcta
     1081 tgccgacaca gatttaatcg gcacgcttta cccattcggc actgcaaaag aggattttct
     1141 ctttgtctat agccaacatt acgacacgct ggaaattaac agcacattcc acgcccccat
     1201 cgggcagaaa gcctttgaag gaatggtgca gaaagcggaa ggtcggctga atttttcggt
     1261 gaaactgcat caagatttca gccatcaacg cacggcgact ttagatcaag cacaattatt
     1321 tcttaatgcg ttagctccgt tcaccgctga agggttattg gcaaatttat ttgttcagtt
     1381 cccaactcaa tttgaacgta tgccacttaa ccgccgttac cttgccgagt tgtgccaatg
     1441 gtttgcggat tatccgttag cgattgaatt tcgccaccca agctggcata ttccggcggt
     1501 gtttgatacc tttgcgaaaa gctctaatct gatttggtgt aatgtggatt acccacaaaa
     1561 tatcggctta ccggcatttc acttttttgc caaccaacgt actgcttatt tacgcttaca
     1621 tggtcgtaac ccgaattggt ggaaagggca gagtgcggca gaacgccacg attaccgcta
     1681 ttcagacgag gaattaaaag gcttggcaga attgcttttt agccgcagaa cggaatttga
     1741 caggctctat ctctactttg aaaacaccac taaaagccac gccttttata atattcaaac
     1801 cctaaaaggc tttttggcgg aaaaaggatt tcaaatcaaa cctgtgccgg agaatttgct
     1861 tgggcagcag agtttgtttt gatgggtaat ttacattgtt gaggagtaaa taaagggcca
     1921 tgccatttag taatggatag ccctta
//
LOCUS       DANLXF010000298         1946 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.299, whole genome shotgun sequence.
ACCESSION   DANLXF010000298 DANLXF010000000
VERSION     DANLXF010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.299"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            17..1669
                     /locus_tag="PXD66_002435"
     CDS             17..1669
                     /locus_tag="PXD66_002435"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HDL6320676.1"
                     /translation="MNGARFITESLKAHGVTTLFGYPGGAIMPTYDAIYDSGLDHLLC
                     RNEQGAAMAAIGYARATGKVGVCIATSGPGATNLITGLGDAALDSIPVVAITGQVASH
                     LIGTDAFQEADVLGLSLACTKHSFIVQNIEELPEILALAFQIAQSGRPGPVLVDIPRD
                     VQLAQTSALPIVYPKEPADAQDPNKLVVAKQLLATAKRPVLYVGGGVSMADGVQAVRN
                     FVKIANIPSVSTLKGLGTIDPTDPLYMGMIGMHGTKAANYAVQECDLLIACGARFDDR
                     VTGKLDTFAPHAKVIHIDIDNAEINKLRQAQVALKGNLIEAVNYLAQPLDIEPWREDV
                     RNLKQQFDFQYQENSGDGNIDAVALLHTLSQRKAKNSIVTTDVGQHQMWSAQHLTYHD
                     PKNYITSAGFGSMGFGLPAAIGAIKARAEDQVILITGDGSIMMNIQELGTLKRADLAV
                     KILLLDNQRLGMVRQWQSLFFHGRHSQTILDDNPDFVLLAKAFDIDGERIEKASEVSD
                     AIDRMFNAKGAYLLHVCIPQEDNVWPLVPPGACNADMLDDEV"
     gene            1679..1897
                     /gene="ilvM"
                     /locus_tag="PXD66_002436"
     CDS             1679..1897
                     /gene="ilvM"
                     /locus_tag="PXD66_002436"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HDL6320677.1"
                     /translation="MEEYQLTLKANKRPETLERILRVIRHRGFEVKCLNVESTDSAFN
                     LNFTLASKRPLSLLSHQLEKLFDIIEIN"
BASE COUNT          555 a          413 c          441 g          537 t
ORIGIN      
        1 taaggacaca acatccatga atggagcaag atttatcacc gagagtttaa aagctcacgg
       61 ggtaacgaca ctttttggct atccgggcgg ggctattatg cctacctatg atgcaattta
      121 tgattcaggc ttagatcatt tactttgccg caatgaacaa ggtgctgcaa tggcagctat
      181 cggttatgcc cgtgcaacag gtaaagtagg ggtttgtatt gcgacctccg gccctggggc
      241 aactaattta atcaccggtt taggcgatgc cgcacttgat tctattcctg tggtagcaat
      301 taccggacaa gttgccagcc atttaatcgg cactgatgcc ttccaagaag ctgatgtact
      361 aggtttgtca ctcgcttgca ctaaacacag ttttattgtt cagaatattg aggagctccc
      421 tgaaatttta gccctcgcat tccaaattgc acaaagtggc agacctggtc cggtgctggt
      481 cgatattcca cgtgatgtgc aactagctca aacttcggct ctgcctatcg tttatcctaa
      541 agaacctgcc gatgcacaag atcccaacaa attagtggtt gccaaacaat tattagccac
      601 cgccaaacgc ccggtacttt atgtcggcgg tggcgttagt atggctgatg gtgtacaagc
      661 ggtcagaaat tttgtaaaaa ttgcaaatat tccgtctgtt tctaccttaa aagggttagg
      721 cactatcgat ccaaccgatc cgctctatat gggaatgatc ggtatgcacg gcacaaaagc
      781 agcaaattac gcggtacaag agtgtgattt actcattgcc tgtggtgccc gctttgatga
      841 ccgtgttacc ggcaaattag acacttttgc ccctcacgct aaagtgattc acattgatat
      901 cgacaatgcg gaaatcaaca agctccgtca agcacaagtt gccttaaaag ggaatttgat
      961 tgaagccgtg aattacttgg ctcaaccgtt agatattgaa ccatggcgtg aagatgtgcg
     1021 taatttaaag cagcaatttg actttcaata tcaagaaaat agtggtgatg gcaatattga
     1081 tgccgtggcg ttattacaca cgctttcaca gcggaaagcc aaaaacagca tcgtcactac
     1141 cgatgtaggg cagcatcaaa tgtggtcggc acaacattta acttatcacg atccgaaaaa
     1201 ctatatcacc tctgccggtt ttggctctat gggttttggc ttgcctgcgg cgattggggc
     1261 aatcaaagct cgagcggaag atcaggttat tttaattacc ggtgatggct cgattatgat
     1321 gaatattcaa gaactcggca cgcttaaaag agctgattta gcggtcaaaa tcctattact
     1381 ggataaccaa cgtttaggta tggttcgcca atggcaatcg ctgttcttcc acgggcgaca
     1441 cagccaaacc attttagatg ataaccctga ttttgtgttg ttggcgaaag cctttgatat
     1501 tgacggtgag cgaattgaga aagcgagcga agtgtcagat gcaatagaca gaatgttcaa
     1561 tgccaaaggg gcttacttac ttcacgtttg tattccacag gaagataacg tttggccact
     1621 ggtaccgccg ggggcgtgta atgccgatat gttagacgat gaggtatagg agaataagat
     1681 ggaagaatat caattaacgc ttaaagctaa caaacgccct gaaaccttag agcgtatttt
     1741 acgggttata cgtcaccgtg gttttgaggt gaaatgctta aatgtagaat caacagacag
     1801 cgcctttaac ctaaatttta cattagcaag taaacgtcca ctctctcttt taagccatca
     1861 attagaaaaa ctgtttgata tcattgaaat caattaatca gacttttcat tcgccgtgta
     1921 aaaaaataca cggctttttt atgctt
//
LOCUS       DANLXF010000299         1939 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.300, whole genome shotgun sequence.
ACCESSION   DANLXF010000299 DANLXF010000000
VERSION     DANLXF010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.300"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..932
                     /locus_tag="PXD66_002437"
     CDS             <1..932
                     /locus_tag="PXD66_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferasee"
                     /protein_id="HDL6320678.1"
                     /translation="KQLPPFQTQFLHPKYWGLWLGLGVFKLILCLPYPILVKIGLGLG
                     KLFTKLGFGKRRMRIARRNLELCFPHYSAEKVEELVEENMKSVGMAIIETGMAWFWSD
                     KRILKWSKIEGLEHLTQRDQERGILLVGVHFLTLELGARIIGLHHQGVGVYRPNDNPL
                     LDWIQFRGRVRSNKAMLDRKDVRGMIRALKNGEMIWYAPDHDYGRRNSVFVPFFAVEE
                     TCTTAGSYMLLRSAPKSLVIPFSPIRNLDYSGYTVKVSPAVDFSQCQNEVETAILMNR
                     VVEREIMAALTQYMWLHRRFKTRPNEGDQSLYD"
     gene            1028..1915
                     /gene="dapA"
                     /locus_tag="PXD66_002438"
     CDS             1028..1915
                     /gene="dapA"
                     /locus_tag="PXD66_002438"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDL6320679.1"
                     /translation="MATPLFHGSIVALVTPMTHGEVDYQDLKKLVEYHIQAGSHAIVS
                     VGTTGESTTLSIDENVKVIKKTVEFADGRIPIIAGTGSNATSEAITMTKLLANSGVAG
                     CLSVVPYYNKPTQEGMYLHYKAIAESTDLPQILYNVPGRTGSDLKPETIGRLAKIENI
                     VAVKEATGDLSRLPLIKQLAGEDFIFLSGDDATGFESMKLGGQGVISVTNNVAAADMA
                     KMCELALAGQFTEAEEINNRLMALHHDLFVESNPIPVKWAAYKLGLISEPNLRLPLTT
                     LSESAQPVVLKALQQAGLI"
BASE COUNT          588 a          306 c          436 g          609 t
ORIGIN      
        1 aaaaacagtt acctccattc cagacacaat ttttacaccc taaatattgg ggattgtggc
       61 tcggtttagg tgtttttaag ttaattttat gtttgcctta tcctatttta gtaaaaattg
      121 gtctgggact aggtaaattg tttactaaat tgggttttgg taaacgtcgt atgcgtattg
      181 ctcgccgtaa tttagagctc tgttttcccc attactctgc tgagaaagta gaggaattag
      241 tcgaagaaaa tatgaaatcg gttggaatgg cgattattga aactggtatg gcttggtttt
      301 ggtcggataa acgtattctt aaatggtcta aaatcgaagg gctagagcat ttaacacagc
      361 gtgatcagga aaggggtatt ttacttgtag gggtgcattt tctgacttta gaactcggtg
      421 caagaattat tgggctacat catcaaggag taggggttta tcgaccgaat gataatccat
      481 tattagattg gatacagttt cgaggacgtg ttcgctcaaa taaagcgatg ttagatcgta
      541 aagatgtgcg tggaatgatt cgagctttaa aaaatggcga gatgatatgg tacgcgcctg
      601 atcacgatta tggaagacga aatagcgttt ttgtaccatt ttttgcagtg gaggaaactt
      661 gtactactgc gggtagttat atgttacttc gttctgcccc aaagagttta gtgatcccgt
      721 ttagcccgat tcgaaatttg gactattcag gctacacagt aaaagtcagt cctgccgttg
      781 attttagtca atgtcaaaat gaagttgaaa cggctatttt aatgaatcgg gtggtagaac
      841 gtgaaattat ggctgcgcta actcaatata tgtggctaca tcgccgtttt aaaactcgcc
      901 caaatgaagg tgatcaaagc ctatatgatt aattttaaat aatttcaaaa aagtgcttgc
      961 atatttaagc tgaattttgc aatctagcaa tgttatcaag cataataaaa aaatacagga
     1021 gatgattatg gcgacccctt tatttcacgg tagtatcgta gcattagtta caccaatgac
     1081 tcatggtgag gtagattatc aggatttaaa aaaattagtt gagtatcata ttcaagcagg
     1141 ttctcacgct attgtatctg ttggtacaac aggtgaatct accacattaa gcattgatga
     1201 aaatgttaag gtgattaaga aaacggttga atttgctgat ggacgtattc caattattgc
     1261 aggaactggt tctaatgcga ccagtgaagc tatcactatg actaaattat tagcaaacag
     1321 tggtgtagct ggttgtttaa gtgtagtgcc ttattacaac aagccaacgc aagaggggat
     1381 gtacttacac tacaaagcga ttgcagaaag tacagattta ccacaaattc tgtataatgt
     1441 gccggggcga acagggagtg atttaaaacc tgaaacaatc ggtcgcttag cgaaaattga
     1501 gaatattgtt gctgtgaaag aagcaacggg tgatttaagc cgcttacctt taattaagca
     1561 attagccggt gaagatttta ttttcttaag tggtgatgat gctaccggtt ttgagtctat
     1621 gaaattaggt ggtcaagggg tgatctcggt caccaataat gttgctgctg ctgatatggc
     1681 aaaaatgtgt gagttagctc ttgccggtca atttactgaa gcagaagaaa ttaataaccg
     1741 cttaatggcg ttacaccatg acttatttgt cgaatctaac ccgattccgg tgaaatgggc
     1801 tgcttataaa ttaggtttaa ttagtgagcc taatttacgt ttaccgctta ccacactgtc
     1861 tgaatcagca cagcctgtag tgttaaaagc attacaacaa gcgggcttaa tttagttatt
     1921 ttttgcaaat caaatttga
//
LOCUS       DANLXF010000300         1920 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.301, whole genome shotgun sequence.
ACCESSION   DANLXF010000300 DANLXF010000000
VERSION     DANLXF010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.301"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(88..1803)
                     /gene="proS"
                     /locus_tag="PXD66_002439"
     CDS             complement(88..1803)
                     /gene="proS"
                     /locus_tag="PXD66_002439"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDL6320680.1"
                     /translation="MRTSQYLFSTLKEMPNDAQVVSHQLMLRAGMIRPMASGLYNWLP
                     TGIKVLKKVENIIREEMNKGGAIEVLMPVVQPAELWVESGRWNDYGPELLRFKDRGER
                     DFVLGPTHEEVITDLVRREVSSYKQLPLNLYQIQTKFRDEVRPRFGVMRSREFVMKDA
                     YSFHTTQESLQETYDVMYQVYSNIFTRLGLDFRAVQADTGSIGGSASHEFQVLASSGE
                     DDVVFSTESDFAANIELAEAVAVGERQAPTAEMQLVDTPNAKTINELVENHGLAIEKT
                     VKTLVVKGATEEQPLIALVIRGDHELNEIKAQKHPLVADPLEFADEAEIKAKIGAGVG
                     SLGVVNMPIPVIIDRSVALMSDFGCGANIDGKHYFNVNWERDVAVPEIADLRNVVEGD
                     PSPDGKGTLQIKRGIEVGHIFQLGKKYSEAMKATVQGEDGKPLVMTMGCYGIGVTRVV
                     AAAIEQHYDDRGIIWPCDEMAPFTVAIVPMNMHKSEKVQAFAEELYANLKAQGVEVIF
                     DDRKERPGVMFADMELIGVPHMVVIGEKNLENGEIEYKNRRSGEKQMIAKESLLAFLK
                     ANVKA"
BASE COUNT          507 a          492 c          353 g          568 t
ORIGIN      
        1 aataatagag tattttatac tttagttaaa tactcgctgc tttatattaa agggcgtatt
       61 ttatacgccc aattttgtta tttataatta agcctttaca ttcgctttta agaacgcaag
      121 taagctctct ttggcgatca tctgtttttc accgctgcgg cggtttttgt attcgatttc
      181 gccgttctcg aggtttttct caccaataac caccatatgc ggcacaccga taagctccat
      241 atccgcgaac atcacgcccg ggcgttcttt acgatcatca aaaatcacct caacgccttg
      301 agcttttaag ttagcgtata actcttcagc gaatgcctgc actttttccg atttgtgcat
      361 attcatcggc acaatcgcca ccgtaaatgg cgccatttca tcacaaggcc agataatacc
      421 acgatcatca taatgttgtt caatcgcagc tgccaccaca cgggtaacgc cgataccgta
      481 acaacccatt gtcatcacta gcggtttacc gtcttcacct tgcacagtgg ctttcattgc
      541 ttctgagtat tttttgccta attggaaaat atgccctact tcaatacctc gtttgatttg
      601 aagtgtgcct ttaccgtccg ggcttgggtc gccttccacc acattgcgta aatctgcaat
      661 ttccggcaca gcaacatcac gctcccagtt cacgttaaag tagtgtttgc catcaatatt
      721 cgcaccgcag ccaaaatctg acatcaacgc cactgagcgg tcaataatca ccggaatcgg
      781 catattcacc acacctaacg agccaacacc ggcaccgatt ttggctttaa tttccgcttc
      841 atcggcaaac tcaagcggat cagccaccaa cgggtgtttt tgagctttaa tttcgtttaa
      901 ctcgtggtcg ccacgaatca ctaacgcaat taacggttgc tcttcagttg cccctttcac
      961 cactaaggtt ttcaccgttt tttcaatggc taaaccgtga ttttctacta attcattgat
     1021 ggttttcgca tttggcgtat ccactaattg catttctgcc gttggtgctt ggcgttcacc
     1081 caccgcaacc gcttcggcta attcaatatt ggcagcaaaa tctgattcgg ttgaaaatac
     1141 cacatcatct tcaccgcttg aggctaacac ttggaactcg tgagaggctg aaccgccgat
     1201 agagccggta tctgcctgca ccgcacggaa atctaagccc aaacgggtga aaatattgct
     1261 gtaaacctga tacatcacat cataggtttc ttgcaggctc tcttgtgtgg tgtggaaaga
     1321 ataggcatct ttcatcacaa attcacgcga acgcattacc ccgaaacgag ggcggacttc
     1381 atcacggaat ttcgtttgaa tttggtataa attcagcgga agttgtttgt acgaagaaac
     1441 ttcacggcga accaagtcgg taatcacttc ttcgtgagtc gggccaagca cgaaatcacg
     1501 ctcgccacga tctttaaaac gtaataattc cgggccgtaa tcattccaac gaccggattc
     1561 cacccataat tccgccggct gaaccaccgg cattaatacc tcgatagccc cgcctttgtt
     1621 catctcttca cgaataatat tttccacttt tttcaacact ttgatcccgg tcggcaacca
     1681 gttgtataaa cctgacgcca tcgggcgaat cattccggca cgcaacatta attgatggct
     1741 aactacttgt gcatcattcg gcatttcttt aagggttgaa aataaatatt gacttgtacg
     1801 cattgctatt cctattcttt ttaaactttg aaacctgaaa aaatctgctt attttaaaac
     1861 aaagcggtta aattttcagg aaattttgca aaatcctata aaaaaataac cgcttgtaag
//
LOCUS       DANLXF010000301         1904 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.302, whole genome shotgun sequence.
ACCESSION   DANLXF010000301 DANLXF010000000
VERSION     DANLXF010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.302"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            303..1835
                     /locus_tag="PXD66_002440"
     CDS             303..1835
                     /locus_tag="PXD66_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HDL6320681.1"
                     /translation="MNLVDYSTSLWSVLPALIALILAIVTRKVIISLSIGILFGAFML
                     TNSNFLDALTYIKNSVVSLVYKGAEEGLNSNNVNIILFLLLLGVLTSLLSISGSNQAF
                     ANWAQKKVKGKRGAKLMAAGLVFFTFIDDYFHSLAVGAIARPVTDKFRVSRAKLAYIL
                     DSTAAPMCVLMPISSWGAYIITLIAGLLATHSITGYSPLGAFMTMSAMNFYAIFAMIM
                     VFFVAYFSFDIGSMARFERQAAHLAELDDEQIETKGHVRDLILPIIALIVATVSMMMK
                     TGADALAESGASFSILGAFENTTVGISLVVGGVAGLIVATICIITSGNISFKNYVRAY
                     GLGVKMMSGAITILFFAWTINGVVSDMQTGKYLSGLVAESIGAEFLPAILFVLAAAMA
                     FSTGTSWGTFGIMLPIGASMAVHSDPALIIPCLSAVMAGAVCGDHCSPVSDTTILSST
                     GAQCNHMDHVISQLPYALLVATASIIGYLVVGFTQSAVLGFIATAVVMAILILLFKEK
                     KH"
BASE COUNT          512 a          312 c          415 g          665 t
ORIGIN      
        1 aacactgtcc tacaaattgt agaggtgcga attcaataag tatttcttct gagtggaaaa
       61 cgatgaaggg gaaggaaagg tgaatttgcc gaaatcaatt aagcgtcatc ttaattggtt
      121 ggggtcgttg tcgaaagaaa cgacactgtc gtagtaaatg ctacggagtg ctactgttag
      181 ggtgggtttc agatatttca catttgcctt cctttcatat ttatttttat aaatatccca
      241 gtagatctct tgtttggcta ttcttattta tacaaattat acacagtcat tttagaggtt
      301 ttatgaatct agttgattat tctacgtcac tttggtcggt attgccggca ctgattgcgc
      361 taatattggc aattgtgaca cgcaaggtga ttatttcact tagcatcggt attctctttg
      421 gtgcatttat gcttactaat agcaattttc ttgatgcact tacttatatt aaaaatagcg
      481 ttgtttcttt agtttataaa ggggctgaag aaggattaaa ttcaaataat gttaacatta
      541 ttttattttt gcttttatta ggggtattaa cgtcattatt aagtatttca ggtagtaatc
      601 aggcgtttgc gaattgggcg cagaaaaagg taaaaggcaa acgaggcgca aaattaatgg
      661 cggcaggttt ggttttcttt acctttattg atgactattt ccacagttta gcagtaggtg
      721 cgattgcccg ccctgttacg gataaattcc gtgtttctcg tgctaaatta gcttatattt
      781 tagattctac cgccgctcca atgtgcgtgt taatgccaat ttcaagctgg ggggcttata
      841 ttattacctt aatagctggc ttattggcta cgcattccat cacaggctat tcgccactag
      901 gcgcatttat gacaatgagc gcaatgaact tttacgccat ttttgcgatg attatggtgt
      961 tctttgtggc atatttctca tttgatattg gttcgatggc gcgttttgaa cgtcaagcag
     1021 cgcatttagc agaattagat gatgagcaaa ttgaaacgaa aggacacgtg cgcgatttga
     1081 ttttgccaat tattgcactt attgttgcta cagtctcaat gatgatgaaa acaggtgcag
     1141 atgcactggc cgaatcaggt gcttcatttt ctatactagg tgcttttgaa aatactacgg
     1201 taggcatttc attagttgtt ggtggtgtgg cagggcttat agttgcaact atttgtatca
     1261 ttacaagcgg taatatttct tttaaaaatt acgtccgtgc ttatggctta ggtgtgaaga
     1321 tgatgtctgg tgcaatcact attctcttct ttgcgtggac aataaacggc gtagtaagtg
     1381 atatgcaaac agggaaatat ctatcaggat tggttgcaga aagcattgga gcagagtttt
     1441 tacctgcaat tctatttgtg ttagccgcag caatggcgtt ttcaacagga actagctggg
     1501 gaacatttgg tattatgttg ccaattggag cctcaatggc agtgcattca gatccagcat
     1561 taattattcc ttgtttatct gcagtaatgg ctggtgcggt ttgtggtgat cactgttctc
     1621 cagtatctga taccactatt ctttcttcaa caggcgcaca atgtaatcat atggatcatg
     1681 tcatttctca attaccttat gctttattag ttgcaacagc ttctattatt ggttacttag
     1741 tggttggctt tacccaatca gcggtattgg gatttattgc tacagctgtt gttatggcaa
     1801 tactcatttt gctttttaaa gaaaaaaagc attaatttcc gaaaatgtga agggaatcac
     1861 agaatgagat ttattgatga gataaaatag cactcaagat atgg
//
LOCUS       DANLXF010000302         1898 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.303, whole genome shotgun sequence.
ACCESSION   DANLXF010000302 DANLXF010000000
VERSION     DANLXF010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.303"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            155..1792
                     /gene="pyrG"
                     /locus_tag="PXD66_002441"
     CDS             155..1792
                     /gene="pyrG"
                     /locus_tag="PXD66_002441"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HDL6320682.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLASILEARGLNVTIMKLDPY
                     INVDPGTMSPTQHGEVFVTEDGAETDLDLGHYERFIRSKMSKANNFTSGKIYSEVLRK
                     ERRGDYLGATIQVIPHITNEIKARVLEGGKGRDVAIVEVGGTVGDIESLPFLEALRQL
                     AVDIGREKTLFMHLTLVPYIPTAGEVKTKPTQHSVKELLSIGIQPDVLICRSDRAIPE
                     NEKKKIALFCNVPERAVISLKDVDSIYRIPELLKSQGLDSFVCDRFRLDCKEADLSEW
                     EQVLYCQANPTGEVTIGMVGKYVELPDAYKSVNEALKHAGLTHRLTVNIKYIDSQDIE
                     TKGTELLAGLDGILVPGGFGYRGVEGKIRTAQYARENKIPYLGICLGMQIALIEYARN
                     VAGLTEANSSEFDKDCSQPVIGLITEWQDAEGNVETRSDESDLGGTMRLGSQQCHLIE
                     GSKARELYGAETIVERHRHRYEVNNKLLPQIEAAGLKVSGLSADRKLVEIIEVPNHPW
                     FIAAQFHPEFTSTPRDGHPLFAGFVKAAKDHQESRKA"
BASE COUNT          555 a          362 c          449 g          532 t
ORIGIN      
        1 ttgaaaaaag aattaatctg actgcttgcc gattttccat tagcaaaaaa atgtaatttg
       61 atgtaaacta ctttcccgtc ctgatagttt cttttttacg aagtaaactg atattctgaa
      121 tttccaacct aatcaatcag caaaggcaat cacaatgaca acgaattata ttttcgtcac
      181 aggcggtgtt gtatcttctt tagggaaggg tattgcggca gcctctttag catctattct
      241 ggaagctcgt ggtttaaatg taaccattat gaagcttgat ccttatatca atgtcgatcc
      301 gggtacaatg agtccaactc agcacgggga ggtatttgtg accgaagacg gtgcggaaac
      361 cgatttggat ttaggtcact acgagcgttt tatccgttca aaaatgagca aagccaacaa
      421 ctttaccagc ggtaaaattt actctgaagt gttgcgtaaa gaacgccgtg gcgactattt
      481 aggtgcaacc attcaggtta ttccgcatat taccaacgag attaaagccc gtgtgttaga
      541 aggcggtaaa gggcgtgatg tcgccattgt agaagtaggt ggcacggtgg gggatattga
      601 atcactgcca ttcttagaag cactccgcca attagcggtg gatatcggtc gtgaaaaaac
      661 cttgtttatg catttaacct tagtgcctta tattccaacc gcaggggaag tgaaaactaa
      721 gccgactcag cattcagtaa aagagttgct ttcaatcggt attcagccgg atgtgttaat
      781 ttgccgttca gatcgtgcta ttccggaaaa tgagaagaaa aaaatcgccc ttttctgtaa
      841 tgtgccggag cgtgcggtta tttcgcttaa agatgtagat tcgatttatc gcattccgga
      901 attattgaaa tcgcaagggt tagattcctt tgtgtgcgat cgtttccgtt tagattgcaa
      961 agaagccgat ctttcagagt gggaacaagt gctatactgc caagcaaacc caaccggcga
     1021 agtgactatc ggtatggtcg gaaaatatgt ggaattacct gatgcgtata aatcggtgaa
     1081 tgaggctttg aaacacgcag gtttaacgca tcgtttaaca gtgaatatta aatacattga
     1141 ttcacaagat attgaaacca aaggcacaga gttattggca ggcttagacg gcattcttgt
     1201 gccgggcggc tttggctatc gtggcgtgga aggcaaaatt cgaaccgcac aatatgcccg
     1261 tgaaaacaag attccttact taggtatctg tttaggtatg cagatcgccc taattgaata
     1321 tgcccgtaat gtggcaggtt taaccgaggc aaattcttct gaatttgata aagattgctc
     1381 acagccggtg attggcttaa ttaccgaatg gcaagatgcg gaagggaatg tggaaacccg
     1441 cagcgatgag tcagatttag gtggtacaat gcgtttaggc tcacaacaat gccatttaat
     1501 tgaaggctca aaagctcgtg agttatatgg tgcggaaacg attgttgaac gtcatcgcca
     1561 ccgttacgaa gtaaacaata agctgttacc acaaattgaa gcggcaggct taaaagtgag
     1621 cggtttatcg gcagatcgta aattagtgga aattattgaa gtgccaaatc acccttggtt
     1681 tatagcagca caattccatc cggaatttac ctcaactccg cgtgatggtc atccattatt
     1741 tgcaggtttt gtgaaagctg cgaaagatca ccaagagagt cgtaaggctt aatcaaactt
     1801 aacaagcggt cgaatttact catttttttg tacaaacgct tgcgtttgaa catcggcttt
     1861 gctgatggca cgaagtgcga gcgaaagcga gtaaattt
//
LOCUS       DANLXF010000303         1827 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.304, whole genome shotgun sequence.
ACCESSION   DANLXF010000303 DANLXF010000000
VERSION     DANLXF010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.304"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            43..252
                     /locus_tag="PXD66_002442"
     CDS             43..252
                     /locus_tag="PXD66_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome alternative rescue factor ArfA"
                     /protein_id="HDL6320683.1"
                     /translation="MSKQKNDYLHQRGQIKESAVKALVTDPLFRQRVERNRKGKGSYR
                     RQEKHKKTIYGQKETPFKNIRLESF"
     gene            complement(301..1023)
                     /gene="bioD"
                     /locus_tag="PXD66_002443"
     CDS             complement(301..1023)
                     /gene="bioD"
                     /locus_tag="PXD66_002443"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HDL6320684.1"
                     /translation="MPSLFITGTDTNVGKTVVTRAIIQTLTKHSFPVIGYKPIACGGD
                     DSLPTEPTQYDYATEDNSDVLTILDSCPTNVSYRDINSYTFIHSSTPVFAALDAVHHI
                     QLEKLNTDLKRLENAYPNVVVEGTYGWLTPINKDYYFADWVMQNNMPVVLVVGIKEGC
                     VNHALLTANEILQRGVKLVSWVANRVNPGLRHYHELIELLTQKINAPLLGQIPYMGHP
                     EHKELSSYIQNPEPLLAYFSKP"
     gene            complement(1071..>1827)
                     /locus_tag="PXD66_002444"
     CDS             complement(1071..>1827)
                     /locus_tag="PXD66_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL6320685.1"
                     /translation="VIGKINAEKDSITQLGNKNIFCNITSKLSPLFHCPILLSEHFQL
                     WLLAESTVGSLINQDNAIFLQLDDTVNLSVLLSGSLLSKQSKMNVDNMLMPRFSHLSD
                     EIFPELDEIHRYKLVNQVTFPAIVRLIDRYLPNNLKSQEQKISWLCQKIDENQPLALA
                     ILSHITDNLAYALMNLVNIFSSEKIMLNSPLIKIKEPLFTQISEKLHKNLLQTNLKID
                     LVTSQYDWNSPLIACSAIKQGIYDGNLIKDRIN"
BASE COUNT          602 a          302 c          325 g          598 t
ORIGIN      
        1 ttttgccaga atacaacctc attttgatat gaggaaaata gaatgagtaa gcaaaagaat
       61 gattacctcc accagcgagg tcaaattaaa gaaagtgcag taaaagcatt agtgactgat
      121 ccacttttta gacaacgtgt agaaagaaat cgtaaaggga aagggagcta tcgcagacaa
      181 gaaaaacaca aaaaaactat ctatgggcaa aaagaaaccc catttaaaaa tattcggtta
      241 gagtcttttt aaatggggtt ttatttgtag cttattgtag ttttataagc ctgataaaca
      301 ctatggttta ctgaaatagg ccaacaacgg ttctggattt tgaatataag aagaaagttc
      361 tttatgctct gggtgaccca tatagggaat ttgcccaagt aaaggcgcat taattttttg
      421 tgtcagtagt tcaattaatt cgtgataatg ccgtagccct ggatttactc tgtttgcgac
      481 ccaacttact aattttacac cacgctgcaa aatttcattc gcagtaagta gtgcatggtt
      541 aacacaacct tccttaattc ccaccactaa aacaaccggc atattgtttt gcattaccca
      601 atcagcaaaa tagtaatcct tgttaatagg agttagccag ccataggttc cttctacgac
      661 aacatttgga taggcatttt ctagtcgttt taaatctgtg tttaattttt ccagttgaat
      721 atgatgaact gcatctaaag ctgcaaatac aggggtactg gaatgaataa aagtatagct
      781 attaatatca cgatagctaa cattagttgg acagctatct agtatggtta atacgtctga
      841 attatcttca gtagcataat catattgagt aggttctgtg ggtaatgaat catcaccgcc
      901 acaagcaatg ggtttatagc caattacagg aaaactatgt tttgtaagtg tctgtatgat
      961 ggctcgggta accacagttt tccctacgtt ggtgtctgtt ccggtaataa ataatgaggg
     1021 cataaatggc tcctttcgaa aatatggctg gtagtttaac caaaaataga tcaatttatt
     1081 ctgtctttga tcaaatttcc gtcataaatt ccttgtttta ttgctgaaca ggcaattaat
     1141 ggactattcc aatcgtattg gctggtaact aaatcgattt ttaggtttgt ttgtaacaaa
     1201 tttttatgca atttttctga aatttgcgta aataacggct cttttatttt gatcaatggc
     1261 gagtttagca taattttttc actagaaaat atattaacta aattcattaa tgcataagct
     1321 aaattatcgg taatatgcga taaaatagct aatgcaagcg gttgattttc atcaattttt
     1381 tgacaaagcc agctgatttt ctgttcctga ctttttaaat tattaggcaa ataacgatca
     1441 atgagtctca caatagctgg gaacgtcacc tgattgacta atttatagcg atgaatttca
     1501 tctagttcag ggaatatttc atcactgaga tggctaaatc taggcataag catattatca
     1561 acattcatct ttgattgttt agacagtaaa ctaccactaa gcaatacact tagattcaca
     1621 gtatcatcta attgtagaaa aatagcatta tcttggttaa ttaatgagcc aaccgtagat
     1681 tctgcaagca accacagctg aaaatgttca cttaataaaa taggacaatg aaacagcggc
     1741 gagagtttag atgtaatgtt acagaatata tttttgtttc ccaattgagt aatactgtct
     1801 ttttctgcat taattttacc gatcaca
//
LOCUS       DANLXF010000304         1803 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.305, whole genome shotgun sequence.
ACCESSION   DANLXF010000304 DANLXF010000000
VERSION     DANLXF010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.305"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(32..383)
                     /locus_tag="PXD66_002445"
                     /pseudo
     CDS             complement(32..383)
                     /locus_tag="PXD66_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596652.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(380..622)
                     /locus_tag="PXD66_002446"
     CDS             complement(380..622)
                     /locus_tag="PXD66_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012341694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL6320686.1"
                     /translation="MYGINLNLNEKSSQTQNARILAHLQNGGRITSLSALDDFGCLRL
                     SARIKDLRDRGHDIQSVFITVPNGKKVKQYFMECAK"
     gene            complement(779..1012)
                     /locus_tag="PXD66_002447"
     CDS             complement(779..1012)
                     /locus_tag="PXD66_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005714943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDL6320687.1"
                     /translation="MEELKAYLNSLTTKEQESFALRCNTTVGYLRKSISRNSRLGAEI
                     CVAIERETKGLVTRKHLIEDWHLRWPELANDKQ"
     gene            1141..>1803
                     /locus_tag="PXD66_002448"
     CDS             1141..>1803
                     /locus_tag="PXD66_002448"
                     /inference="COORDINATES: protein motif:HMM:NF012920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL6320688.1"
                     /translation="MNELRLLRQKKLKRLIDEVSEGNIADFARKIGKEPSYVARMLYP
                     SDKAGAKPIGEKMALHICHKFVLPLDWFNRDDDGEFIQINNGEEDNIILSVLNIQASA
                     GIGAKNGDVMEVIREIKFNPEQFYNLFRGITPEHIELISVKGDSMEPTFSHGDLLFVD
                     VTIQEYDGDGVYVFTCDDYIFVKRIQKTGRHFTILSDNDRYKPWQLDQEIYIHGKVKV
                     HQS"
BASE COUNT          574 a          332 c          327 g          570 t
ORIGIN      
        1 aaatccgagc tttttcggat ctgtttccga gctaactcgg atttatttcc gagtgtcgga
       61 tttaaatccg agttaaactc attccaagtc ttcccttttt cagttaatcg aataaggtct
      121 ttatctccgt gtttttgata cacgatcaac ccttttttgt gaaactcgac aaaatggcga
      181 tagacggtat caggctttga gtaaaacaaa ggtagttctt cgatcaccgc attacgggaa
      241 acccagtaat aaaccacgcc atcaataatg atttctttcg cccaagcagg agcttgattc
      301 agtaaatcaa ataatgctcc ttgattagca tttaagttcc attcaaggca ttttgcattg
      361 ttgatgtaag tgctaaatct catttcgcac actccataaa atactgcttc actttcttgc
      421 cattcggcac tgtaataaat acgctttgaa tatcgtgacc acggtcacgc aaatctttaa
      481 tgcgagcgga taaacgcaag cagccgaaat cgtctaacgc agaaagcgaa gtaatacgac
      541 cgccattttg aagatgtgcc aaaattcttg cattttgggt ttgacttgat ttttcgttta
      601 ggttaagatt tatcccatac atataggaaa cctccttagt aattagccac cgttacagcg
      661 gtggtttttt taattgttaa aaacccacca cgcaagcagc ataaaaattg ccactctaat
      721 aaaaattgaa tttttactgt ggtagaatga tttgagtttt ttagaaaaaa ctttcccttt
      781 attgcttgtc atttgccaat tccggccagc gtaagtgcca atcttcaatt aaatgctttc
      841 tagtaactaa accttttgtt tcacgctcta tcgcaacgca aatttccgca cctaatcgag
      901 aatttcttga gatcgatttt cttaaatacc caactgtcgt attacaacgc aaggcaaaac
      961 tctcttgctc ttttgtggtt aaagaattga ggtatgcttt taattcttcc attgtgagtc
     1021 ctcttttaaa aacgccaaaa taataccatt aggtaaaaat aatatcaata ccatttggtt
     1081 tgtttacctt ttagtaaata agagtacaat tactaaaaag taatccttag aggaggttat
     1141 atgaatgagt tgagacttct tagacaaaaa aaattgaaac gcctaattga tgaagtgagt
     1201 gagggaaata ttgctgactt tgcaagaaaa ataggtaaag agcctagcta tgtagcaaga
     1261 atgttgtatc cgtccgataa agctggagct aagcctattg gtgaaaaaat ggcactccat
     1321 atttgccata aatttgtgct accccttgat tggtttaatc gagatgatga cggcgaattt
     1381 attcaaatca acaatggtga ggaagataat atcatcttaa gtgtattgaa tattcaagca
     1441 tcggccggta taggagcaaa aaacggtgat gtgatggaag tcattagaga aattaaattc
     1501 aatccagaac aattttataa cctatttaga ggtattacgc ctgaacatat cgagctgatc
     1561 agtgtaaaag gcgattctat ggagccaaca ttctcacacg gtgatttgct ttttgttgat
     1621 gtaactatcc aagaatacga tggcgatggt gtgtatgttt ttacttgcga tgactatatt
     1681 tttgtaaaaa ggattcaaaa aacaggcaga cactttacta ttttatctga taatgaccgc
     1741 tataaacctt ggcaattaga tcaagaaatt tatattcacg gcaaagttaa agtccaccaa
     1801 agc
//
LOCUS       DANLXF010000305         1802 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.306, whole genome shotgun sequence.
ACCESSION   DANLXF010000305 DANLXF010000000
VERSION     DANLXF010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.306"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(252..>1802)
                     /locus_tag="PXD66_002449"
     CDS             complement(252..>1802)
                     /locus_tag="PXD66_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase/relaxase domain-containing protein"
                     /protein_id="HDL6320689.1"
                     /translation="VHDIGKVVVDIEITLKDGSRWFAWNGLPPQPYKFKYIKGRDYHL
                     HPVLGGFLANQLIPKSAFDWLGQYPEVISSLMYAMSGHNDKAGLLSEIVQKADQHSVT
                     LALGGDVNKLVQRPVNSLSKQLVLALRYLLEQKIKLNTPKGPADGWFTEEGLWLVSKT
                     AADNIRAYLLGQGISVPKDNPKLFDEMQSLGIVEESPAGGAIWSCRIQADSGWTPANT
                     FTLLRIKPEVAWENINDRPNYFAGKVKIVEQITEESNIETQSTLVDGTTKPAPLPSVQ
                     TGGIIASKEVEISQVETNSDNAPSSEIIINDENDLTSFVLDMFAPTESSEPSLDDISI
                     GKGQEIASMPSKQEVQDKSTTKKLKVTQSAVETPETLTLSELQLQNKENSITGDNFVK
                     WLKSGIATNKFAINKPTAKLHIVEDHLFLVTPVIFEMYLQETGQPYDKETINNLQYDF
                     QGLGIHSPKNTMTNGKPDTINFWKCDVIGPRKTSSLTGYLIKNTRLFFGDKVLLNNHC
                     LTLQKEET"
BASE COUNT          502 a          359 c          346 g          595 t
ORIGIN      
        1 aaagttatat aaggctttta aatgtctgac ataattattc cacgttactg ctttgatttt
       61 atcgccaaca atttgattac gccattcaat gagcatatcg gtggtaatgt cattggcatt
      121 taactttgga taacgctttt tgataattcg gattacgttg ttgtagctcc gtttggtatc
      181 cggacgaaga tagtgacgat agaaaaaaaa atcggtctag tagcgaatca aaagagatag
      241 aagttatcat tttaagtttc ctctttctgt aaagttaaac agtgattatt taatagtacc
      301 ttatccccaa aaaagagacg ggtatttttt atcaaatatc ctgttaaaga ggaagtcttt
      361 ctcggtccaa tgacatcaca tttccaaaaa ttgatggtat ccggcttacc gttggtcatt
      421 gtgtttttag gtgaatgaat ccctaatcct tgaaaatcat actggagatt attaattgtt
      481 tccttatcat aaggctgtcc agtctcttgc aaatacattt caaaaataac gggggtaact
      541 aaaaacaggt ggtcttcaac aatatggagc ttggcagtag gcttgttaat tgcaaatttg
      601 ttggtcgcaa tacctgattt gagccacttt acaaagttat ctcctgtaat tgagttctcc
      661 ttattctgta actgtagctc tgatagtgtt aatgtttcgg gcgtttctac tgcactttgt
      721 gttactttga gctttttagt ggtagattta tcctgtactt cttgtttgga tggcatactt
      781 gcaatttcct gtcccttacc aattgaaata tcatcaaggc ttggttcgga actttccgta
      841 ggtgcaaaca tatccagcac aaaggaggtt aaatcatttt catcatttat gatgatttct
      901 gaggatggtg cattatccga gtttgtctct acctgagata tttcaacttc tttagaggca
      961 ataatgcccc ctgtctgaac actcggtaat ggagctggtt tagtggtccc atcgactaat
     1021 gtagactgag tctctatatt actttcttct gttatctgct cgactatttt aacttttccg
     1081 gcaaaataat ttggtcgatc attaatattc tcccaagcca cttctggttt aattctcaat
     1141 aatgtaaagg tatttgctgg cgtccaacct gaatcagctt gaatacggca actccaaatt
     1201 gctccaccag ccggtgattc ttctacaata ccgagagact gcatttcatc aaataacttt
     1261 ggattatcct taggcactga aattccctgc cccagtaagt aagctcggat attatcagca
     1321 gcagttttgc taacaagcca caatccttcc tcagtgaacc aaccgtctgc cggacctttg
     1381 ggtgtattta actttatttt ctgttccagc aaataacgta gtgccaagac cagttgcttt
     1441 gacagagaat tgacaggacg ttgaaccagt ttatttacat cgccaccgag tgcgagggta
     1501 acactatgct gatcggcttt ctgcacgatc tcagataaaa gcccggcttt atcgttatgc
     1561 ccagacattg catacatcag tgaagaaatg acttctggat actgtcccag ccaatcaaac
     1621 gcagattttg gaataagttg atttgcaagg aagcctccta gcaccggatg aagatggtag
     1681 tctcgacctt tgatgtattt gaatttataa ggttgaggtg gtaacccatt ccaagcaaac
     1741 caacgtgaac cgtctttgag tgtaatttca atatcaacga caactttgcc aatatcgtga
     1801 ac
//
LOCUS       DANLXF010000306         1770 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.307, whole genome shotgun sequence.
ACCESSION   DANLXF010000306 DANLXF010000000
VERSION     DANLXF010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.307"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..953)
                     /gene="ssb"
                     /locus_tag="PXD66_002450"
     CDS             complement(<1..953)
                     /gene="ssb"
                     /locus_tag="PXD66_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL6320690.1"
                     /translation="MAGINKVIIVGRLGNDPELRTMPNGDSVAKISVATSTEWTDKTS
                     GEKKQATEWHSIIAFRNLADIIGKYLKKGSQVYVEGKLRTRKWQAQDGTDRWTTEIIA
                     DQLQMLGSSNSGNSNNNWATEPAGNPPPTNPYNHVMTDSESRDFDDDIRVRWITKEQA
                     KQFINNISTDWMKTICSFALLTGARRTEILSMTWDKIDFIRKVAIVSNDIAKSEKARS
                     LLLNDDAIELLQKQRGKNPKYVFVGSKGQALQDINRKIFNKATEKCLLVDFHFHDLRH
                     TWASWHVQAGTPLFTLKELGGWETLEMVKKYAHLNADHLLN"
     gene            complement(956..1153)
                     /locus_tag="PXD66_002451"
     CDS             complement(956..1153)
                     /locus_tag="PXD66_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320691.1"
                     /translation="MARKIDAGVEIVEVDKRFIAKFFYDGKLQHTTYPQYNRQNAIML
                     INRKIERFNIGRDKPIKLYKE"
     gene            complement(1137..1322)
                     /locus_tag="PXD66_002452"
     CDS             complement(1137..1322)
                     /locus_tag="PXD66_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320692.1"
                     /translation="MNKMKQLAPFALLILLLGIVGRMDYDDHIQMQRYKCERNQGFWQ
                     MESNGNQYCGGKYGTKN"
BASE COUNT          420 a          381 c          366 g          603 t
ORIGIN      
        1 tggttgagta aatggtctgc attaagatgg gcatatttct ttaccatctc taatgtttcc
       61 cagccaccca attcttttaa ggtaaataat ggtgtgccgg cctgaacgtg ccaactggcc
      121 caagtatgcc ttaaatcatg gaaatggaaa tcaaccaata aacatttttc agtcgctttg
      181 ttaaatattt tgcggttaat atcttgtaat gcctgccctt tgcttcctac aaaaacatat
      241 ttaggatttt tccctctctg cttttgtaat aattcaattg catcatcatt aagtaaaagc
      301 gatctcgctt tttcagattt ggcaatatca ttcgaaacaa tcgctacttt acggataaaa
      361 tctattttat cccaagtcat agaaagtatt tctgttcgcc ttgctcctgt taataaagca
      421 aaagagcaga tagttttcat ccaatcagta ctaatattat taataaactg cttggcttgt
      481 tctttggtaa tccaacgaac ccggatatca tcatcaaaat ctcgtgattc tgaatccgtc
      541 attacgtggt tatatggatt ggttggtggt gggtttcctg ccggttcggt tgcccaatta
      601 ttattgctgt ttccgctgtt tgagctaccg agcatttgta gctgatcggc gatgatttcg
      661 gtcgtccagc ggtctgtgcc gtcttgggct tgccatttgc gagtgcgtag cttgccttcc
      721 acatacactt gcgagccttt tttcaggtat ttgccgataa tgtcagctaa attccgaaag
      781 gcgataatgc tatgccactc tgtggcttgt tttttctcgc ctgaggtttt atccgtccat
      841 tcggttgaag ttgctacact gattttggcg actgaatcgc catttggcat tgttcgtagt
      901 tccgggtcgt tacctaggcg accgacaata atcactttgt taattcctgc cattgttatt
      961 ccttataaag tttgattggt ttgtctctgc caatgttgaa ccgctcaatt ttgcggttaa
     1021 tcaacataat tgcgttttgg cggttgtatt gtggataggt tgtgtgctgt aatttgccgt
     1081 cataaaaaaa cttggcgata aatcgtttat ctacctcaac aatctcaaca ccggcatcaa
     1141 tttttcgtgc catatttccc tccgcagtat tggttgccgt tgctttccat ttgccaaaag
     1201 ccttgatttc gttcgcattt gtaacgttgc atttgtatgt ggtcgtcata atccattcgc
     1261 ccgacaatgc ctaatagcaa aattagcaag gcaaatgggg caagttgttt cattttgttc
     1321 ataggattgt cctttagggt taaaaaaagc ccacgtttta cggtgggcaa atggagcgac
     1381 ttgcgtctag ttgtgggtat tccgaaacac acttggtgaa atgagctttg gaataaatgc
     1441 gtgtctgccc acgccgtcgc ccgcccggct ttcgccattc cgtcgggtgc tgcttgtatc
     1501 cgtacttact caaacttgag ataagcgtga ttgcttagga ctgataccag tgttgccttt
     1561 tcagctctcc acaccttgcg agtgtgttgc ctaaaccttg ccacctcttt ttggcaagtc
     1621 gttcaaattt gtatgtcagg gtctaaccaa ccttactgag ttataaagca ttactttaca
     1681 actgcccgag gttgttaata tcgtccgcac aagcaccgaa ttgttaaaga gcattgagcc
     1741 gtagctcgtt ttgatgaatt aagtttaaga
//
LOCUS       DANLXF010000307         1758 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.308, whole genome shotgun sequence.
ACCESSION   DANLXF010000307 DANLXF010000000
VERSION     DANLXF010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.308"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..207)
                     /locus_tag="PXD66_002453"
     CDS             complement(<1..207)
                     /locus_tag="PXD66_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL6320693.1"
                     /translation="MIITMKDIAKEANVSLGTVSNVLNGKSNVSLAKIEKVQAAVEKL
                     GYRKNIQASTLKSGTSNKIAIILPN"
     gene            complement(369..1709)
                     /gene="gdhA"
                     /locus_tag="PXD66_002454"
     CDS             complement(369..1709)
                     /gene="gdhA"
                     /locus_tag="PXD66_002454"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDL6320694.1"
                     /translation="MSDLNALFQKIKQRDPNQAPFHQAVEEVFGSLAPFLAKNPKYTQ
                     QGLLGRIVEPERVISFRVTWVDDKGQVQVNRGYRVQMNSAIGPYKGGIRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMSELYRHIGADTD
                     VPAGDIGVGGREIGYMFGQYKKLRNEFTSVLTGKSLTWGGSLIRPEATGYGAVYFAES
                     MLATKGQQIEGKSVVISGSGNVAQYAAEKVIQKGGKVLTVSDSNGYVLFPESGMTEAQ
                     LQALLELKNERRERLSVYATEQGLKYFDGQKPWGVKCDIALPCATQNELDTEDAKELV
                     KNGCICVAEGANMPTTLGGVEVFIGAKILYAPGKAANAGGVATSGLEMSQNAIRLSWS
                     REEVDQRLFNIMKAIHENCVENGTEADGFVNYVNGANIAGFKKVANAMLEQGIV"
BASE COUNT          495 a          401 c          306 g          556 t
ORIGIN      
        1 attgggcaat ataatagcaa ttttattact tgtgccactt tttaacgtag aggcttgaat
       61 atttttacga taacccaact tttcaacggc agcttgtact ttttctattt tagctaagct
      121 aacattactt tttccgttta atacattcga tactgttcct aacgaaacat ttgcttcctt
      181 tgcgatatct ttcatcgtaa tgatcatata ctgccctttc acatactgct aaaactgaga
      241 ttggcataga ttttaccata attggcaaat gaaatacgat ttcaatttag cttgtctcta
      301 gctacaacct aaaataaaaa aactgcctcg atatatcgag gcagttcttg aataataggg
      361 ataataaatt aaacaatacc ctgttctaac atcgcatttg ccactttttt gaaaccagca
      421 atattcgcac cgtttacata gtttacgaaa ccatctgctt cagtaccatt ttctacgcag
      481 ttttcgtgaa ttgctttcat aatgttgaat aaacgctgat ctacttcttc acgactccaa
      541 gatagacgaa ttgcattttg gctcatttct aaacctgagg ttgccacacc acctgcgtta
      601 gccgctttac ccggagcata aaggattttc gcaccgataa agacttcaac accacctaat
      661 gtggttggca tatttgcacc ttctgccaca caaatacagc cgtttttcac taattcttta
      721 gcatcttcgg tatctaattc gttttgggtt gcacaaggta aagcgatgtc acatttcaca
      781 ccccacggtt tttgaccgtc aaagtatttt aagccttgtt ctgttgcata cacagataag
      841 cgttcacggc gttcgttttt caactctaat aatgcttgta attgtgcctc tgtcataccg
      901 gattcaggga ataagacata accgttagaa tctgaaaccg ttaatacttt accgcctttt
      961 tgaatcactt tttcagctgc atattgtgcc acgttacccg aaccggaaat taccactgat
     1021 tttccttcga tctgctgacc tttagtcgct aacatagact ctgcaaaata caccgcacca
     1081 taaccagttg cttccggacg aattaagcta ccaccccaag tcagcgattt accggttaat
     1141 actgaagtaa attcattgcg taattttttg tattgaccga acatatagcc gatttcacga
     1201 cctcccacgc cgatatcgcc tgcaggaacg tcagtatcag caccaatgtg gcgatataat
     1261 tcgctcataa acgcttggca gaaacgcatt acttcggcat cagatttgcc tttcgggtca
     1321 aagtctgaac cacctttacc accgcccatt ggcaatgtcg ttaacgcatt tttgaatact
     1381 tgctcaaagg ctaagaattt taatacgcct aaatcaacgg ttgggtggaa acgaataccg
     1441 cctttgtatg gaccgattgc tgagttcatt tgtacacggt aaccacggtt tacttgcact
     1501 tgacctttat catcaaccca agtgacacgg aagctaatta cacgctcagg ctcaacaata
     1561 cgccctaata aaccttgctg ggtatatttc ggatttttag ctaagaaagg ggcaaggcta
     1621 ccgaacactt cttcaaccgc ttgatggaaa ggggcttggt tcggatcacg ttgtttgatt
     1681 ttttggaaaa gggcgtttaa atcgctcata ttatactcct gaataaattg atgatgttgt
     1741 gtatttaaga tgagcgaa
//
LOCUS       DANLXF010000308         1751 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.309, whole genome shotgun sequence.
ACCESSION   DANLXF010000308 DANLXF010000000
VERSION     DANLXF010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.309"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..359
                     /locus_tag="PXD66_002455"
     CDS             <1..359
                     /locus_tag="PXD66_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL6320695.1"
                     /translation="ESYSINGAVGLLASALENLIRNGQKYAKKMLSVSMNVEDGHLFL
                     TVEDDGEGVPENEYENILKPFYRVDEARARETGGAGLGLAIVQNVVKQHQGTIHLDKS
                     NMGGLKIELKLPLWRS"
     gene            409..>1751
                     /locus_tag="PXD66_002456"
     CDS             409..>1751
                     /locus_tag="PXD66_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL6320696.1"
                     /translation="MKLIEIENLNKYFGLGQTRAHILKNINLSIERGDFVAIIGASGS
                     GKSTLMNIIGCLDTASSGICRIDGKETQTMSADELSDLRQRKLGFIFQRYNLLSALNA
                     NENVALPAIYAGMDSSSRKKRADTLLTKLGLSDKTQHKPNELSGGQQQRVSIARALMN
                     GGEIILADEPTGALDSKSGETVLEILRDLHYEGHTIIMVTHDPNIAAQASRIVEIKDG
                     QIIRDERQRTDVQETALNKTTTQKPRFWNQLFESFKMATSAIMAHKMRAILTMLGIII
                     GITSVISVVALGRGSQQQILSNINNLGTNTMDIMNGTGFGDRRANLTKNLTISDALML
                     SRQNYVESTTPSSNVSATLIYGNTAVTGSVRGVGEQFIDVKGLKLISGRFFSAEEVKN
                     VAQVIVIDPNTQKDLGLTEPVEGKTILVDKKPLQIIGVAQQSNNMNQSSLTLWAP"
BASE COUNT          576 a          339 c          379 g          457 t
ORIGIN      
        1 ccgaatctta ttctattaac ggggccgtag gtttactggc tagtgcttta gaaaatttga
       61 tccgaaacgg ccaaaaatat gcaaaaaaaa tgctgtcggt ctcaatgaat gtagaagatg
      121 gtcatttatt tctcacagtt gaagatgatg gcgagggggt tcctgaaaac gaatatgaaa
      181 atattcttaa acctttttac cgcgtagatg aagcaagggc aagagaaaca ggaggagccg
      241 ggctaggatt agcgattgtg caaaatgtag ttaaacagca ccaaggtact attcatttag
      301 ataaaagtaa tatgggagga ttaaaaatcg aactgaaact tcccctttgg cgttcctaag
      361 gcaaaaagca tatcgtttgt aaaaaacagt cgaaaaataa ccgcttgtat gaaattaatt
      421 gaaatagaaa atctcaataa atactttggc ttggggcaaa cccgagccca tattctaaaa
      481 aatatcaatc tcagtattga acggggtgat tttgtcgcaa ttataggtgc ttcaggttct
      541 ggcaaatcta ccttaatgaa cattattggc tgtttagata ctgccagctc aggtatttgc
      601 cgtattgatg gcaaagagac ccaaacaatg tctgcagatg aactgtctga tttacgccaa
      661 cgtaaactgg ggtttatttt ccaacgctat aatctccttt cagctttgaa tgcaaacgaa
      721 aatgttgccc tacctgcaat ttacgccgga atggatagct catcacgcaa aaaacgagct
      781 gatacgttat taacgaagtt aggattatca gataaaaccc aacataagcc gaatgagtta
      841 tctggggggc aacagcaacg agtcagcatt gcacgagcct taatgaacgg cggtgaaatc
      901 attcttgcag acgagcctac cggtgcatta gattctaaaa gtggcgaaac tgtattggaa
      961 atcttgcgag atctacacta cgaaggtcat accattatta tggtaacaca cgacccaaac
     1021 attgccgctc aagccagccg tatcgttgaa attaaagatg ggcaaatcat tcgagatgaa
     1081 cgccaacgta ctgatgttca agaaacggct ctcaataaaa cgacaacaca aaaacctcgc
     1141 ttttggaatc aactgtttga atccttcaaa atggcaacca gtgcaattat ggcgcataaa
     1201 atgcgggcaa tcctcacgat gctgggcatt attattggca ttacctctgt gatttccgta
     1261 gtcgctctcg gtcgaggctc gcaacaacaa atcctttcta atatcaacaa cctaggcaca
     1321 aatacgatgg atattatgaa tggaaccggc tttggcgata gaagagccaa tctgactaag
     1381 aatttaacca tcagtgatgc cttaatgttg agtcggcaaa actatgtgga aagcactact
     1441 ccttcaagca atgtcagtgc cacattgatt tatggtaata ccgcagtaac cggttcagta
     1501 cgaggtgttg gagagcaatt tattgatgtg aaaggattaa agctgatttc agggcgcttt
     1561 ttcagtgccg aggaagtaaa aaatgtagcg caggttattg taattgaccc taacactcaa
     1621 aaagatttgg ggctaacaga gcctgttgaa ggtaaaacga ttttagtgga taaaaaaccg
     1681 ttacaaatca ttggtgtggc gcaacagtca aacaatatga accaaagtag ccttacctta
     1741 tgggcgcctt a
//
LOCUS       DANLXF010000309         1715 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.310, whole genome shotgun sequence.
ACCESSION   DANLXF010000309 DANLXF010000000
VERSION     DANLXF010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.310"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..767
                     /locus_tag="PXD66_002457"
     CDS             <1..767
                     /locus_tag="PXD66_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FNR family transcription factor"
                     /protein_id="HDL6320697.1"
                     /translation="VSEPKTSGRHTCTIHCQNCSISQLCLPFTLNETELTQLDNIIER
                     KKPIQKSQVIFKLGDELRSLYAIRSGTLKAYTLSESGEEQITGFHLPGDLIGFDAITN
                     MQHEGYAQALETSMICEIPFDILDDLAGKMPKIRHQIMRLMSNEIKSDQEMILLLSKM
                     NAEEKLAAFLYNLSQRYSARGFSAKEFRLTMTRGDIGNYLGLTIETISRLLGRFQKSG
                     MISVQGKYIIINRMEELAELAGAIKPQQNIIAQTTA"
     gene            911..1702
                     /locus_tag="PXD66_002458"
     CDS             911..1702
                     /locus_tag="PXD66_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL6320698.1"
                     /translation="MGILTGKRILVTGLASNRSIAYGIANAMKQQGAELAFTYLNDKL
                     KPRVEEFAKEFGSDIVLPLDVATDESITECFTELSKHWEKFDGFVHAIAFAPGDQLDG
                     DYVNAATREGYRIAHDISAYSFVAMAQAARPFLNENAALLTLSYLGAERAIPNYNVMC
                     LAKASLEAATRVMAADLGKEGIRVNAISAGPIRTLAASGIKNFKKMLSAFEKTAALRR
                     TVTIDDVGNSAAFLCSDLASGVTGEVLHVDAGFSVMAMGELGDEE"
BASE COUNT          529 a          351 c          333 g          502 t
ORIGIN      
        1 ttgtatcaga acctaaaaca agcggacgtc atacttgcac aattcattgt caaaattgca
       61 gtattagcca actatgctta ccttttactc ttaatgaaac ggaattaact caactagata
      121 atattattga gcgtaaaaaa ccgattcaaa aatcgcaagt tatttttaag ttaggtgatg
      181 aactgcgttc gctttatgct atccgttctg gaacattaaa agcctatacc ttaagtgaaa
      241 gtggtgaaga gcaaattacc ggtttccatt tacctggcga tctaattggc tttgatgcaa
      301 ttaccaatat gcagcacgaa ggctatgctc aagcactcga aacctcaatg atttgtgaga
      361 ttccattcga tattttagat gatcttgccg gcaaaatgcc aaaaattcgc catcaaatta
      421 tgcgtttgat gagtaatgaa attaaaagcg atcaagaaat gattctattg ctctcaaaaa
      481 tgaatgcgga agaaaaacta gctgcatttt tgtataatct ttcccaacgt tactcagccc
      541 gtggtttctc tgcaaaagag ttccgtctaa ctatgacccg tggagatatc ggcaattatc
      601 ttggtttaac cattgaaact atcagccgtt tattagggcg tttccaaaaa agcggtatga
      661 tttcagtaca aggaaaatat attattatta accgtatgga agaattagcc gaattagccg
      721 gggcaatcaa accgcagcaa aatattattg ctcaaactac cgcttaattt atcttaccaa
      781 atcccttttt gaaacctcgt tcaaaaaggg atttctgatc cgatgagccg ctaactccat
      841 ttgcccgttt ttcctatatc cgttataatc gacacaattt tgccccaatc aatataaaag
      901 aggaattttt atgggtatct taactggtaa acgtatttta gtgacaggtt tagcaagcaa
      961 ccgttcgatc gcttacggta ttgcaaacgc aatgaagcaa caaggtgcag aacttgcatt
     1021 cacctactta aacgataaat taaaaccacg agttgaagaa tttgccaaag aatttggctc
     1081 agacattgtc cttccgttag atgtagcaac cgatgaaagc attactgagt gctttactga
     1141 attaagcaaa cattgggaaa aatttgacgg ttttgttcac gctatcgcat ttgcaccggg
     1201 cgatcaatta gacggcgatt atgtaaatgc cgcaacccgt gaaggttatc gtattgcaca
     1261 cgacatcagt gcctatagct ttgttgcaat ggctcaagct gctcgcccct tcttaaatga
     1321 aaatgccgcc cttttaacct taagttactt aggtgcagag cgtgcaatcc ccaactacaa
     1381 tgtaatgtgc ttagccaaag cctctttaga agctgcaacc cgtgtaatgg cagccgattt
     1441 aggtaaagaa ggtattcgtg taaatgcgat ttcagccggc ccgatccgta cattagctgc
     1501 ttcaggcatt aaaaacttca agaaaatgct ttctgctttc gagaaaaccg ctgctttacg
     1561 ccgcaccgta actattgatg atgtaggtaa ctctgcagct ttcttatgct cagatttagc
     1621 ctctggtgta acaggtgaag tgttacacgt tgatgccggc ttcagcgtaa tggcaatggg
     1681 tgaattaggc gacgaagagt aattcctata aaaat
//
LOCUS       DANLXF010000310         1714 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.311, whole genome shotgun sequence.
ACCESSION   DANLXF010000310 DANLXF010000000
VERSION     DANLXF010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.311"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            61..279
                     /locus_tag="PXD66_002459"
     CDS             61..279
                     /locus_tag="PXD66_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module killer protein"
                     /protein_id="HDL6320699.1"
                     /translation="MMREQTTNQIIITDNFSKWLENLKNPIAKATVARRIKNAINGNF
                     GDHKSLAGTGGLYEMRIATGAGYRIYYA"
     gene            392..715
                     /locus_tag="PXD66_002460"
     CDS             392..715
                     /locus_tag="PXD66_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative addiction module antidote protein"
                     /protein_id="HDL6320700.1"
                     /translation="METIQINEQAAEKHGLRPFDVAEFLTDEATIQAYLAEVLKEGDQ
                     DEFLEALNDVARARGMAELAKQTGLGRESLYKTLAKGSKPRFDTIQKILGAFNLVLIP
                     QVKNG"
     gene            1110..>1714
                     /locus_tag="PXD66_002461"
     CDS             1110..>1714
                     /locus_tag="PXD66_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320701.1"
                     /translation="MADHSQFISRLENRIEPKISFQELIQELSNYTNKPFSHIADKLK
                     SLIFSFQQNGIYTNAFVNCTLFEDGESYAIEPKTLHFKDAFDQPKGVLSDVIAQNSID
                     VEHLNAYYVTAREITQLIKTQSPTLLDKLTTKQTQSEISQIQNDYISVYDFIEWASKH
                     YSSLSETANDLNRLLKDKNIQLYRQYGGISPSIDKDNTNLKA"
BASE COUNT          619 a          321 c          314 g          460 t
ORIGIN      
        1 acccttgctc actgtatctt aatggataca ataagcaata aatagaataa gaagccggca
       61 atgatgagag agcaaaccac taatcaaatt attattaccg ataatttttc aaaatggctt
      121 gagaatttaa agaaccctat cgcaaaagca acggtagcaa gacggataaa aaatgcgatt
      181 aatggcaact ttggcgatca taaatcttta gccggtacag gcggacttta tgaaatgcgg
      241 atagcaacag gagccggata tagaatttat tacgcataac aaggaaatgt aatttatatc
      301 ttgttacacg gcggcgataa atcaacacag caagccgata tagagaaagc gaaagcatta
      361 tgggctgaat taaaaactac aggagaaagc aatggaaaca atacaaatca atgaacaggc
      421 agcagaaaag cacggtttac gcccttttga cgtggcggag tttttaaccg atgaggcaac
      481 tattcaagcc tatttagcgg aagtgttaaa agagggcgac caagacgaat ttttagaagc
      541 cttgaacgat gtagcaagag caagaggaat ggcggagctg gccaagcaaa cagggctagg
      601 cagagagagc ctatataaaa ccctagccaa aggcagcaaa ccacgctttg acactattca
      661 aaaaattcta ggggcattca atttagtgct tattccacaa gtcaaaaatg gctaaaactt
      721 atcacttgct acctgtcaaa acctcacttt taaatagccg ttgatggata ttgacggctt
      781 tttatttgct ccttgcgtgc gtactgctcc gaaaattccg cactattcgc aaaaccttaa
      841 tgaaatatga ccgcttgaat gctctttttt atagcgtccg gaaacccatc tttagcctat
      901 tttgatttac tgtaataaaa cacagcttat tacttgtaat ttatctgaat aattagcaaa
      961 atacaaccta tcaagttcaa tccgtaacta tacggattta ttcaaattag cgtggatagt
     1021 ggcggagtaa ttaaccggca cgaaaggaga aagcccttta tttctctttc cacgctctca
     1081 cagtaaaggg atacgttaaa ggggcgctta tggcagatca cagtcaattt atttctaggc
     1141 ttgaaaatcg tattgagccc aaaatcagct ttcaagaact gatacaagaa ttatcaaact
     1201 atacaaataa acctttttca catatagcgg ataaattgaa aagcctcatt ttttcctttc
     1261 agcaaaatgg aatatatacg aatgcttttg taaattgcac tctctttgaa gatggtgaaa
     1321 gctatgctat tgagcccaaa acactacatt tcaaagacgc tttcgatcag cctaaaggcg
     1381 ttttaagtga tgttattgct caaaattcta ttgatgttga acatttaaat gcttattacg
     1441 taacagcaag agaaataaca cagctgatta aaacacaatc gccaaccttg ttagataaat
     1501 taaccaccaa gcaaacacaa tcagagatta gccaaattca aaatgattat atttctgttt
     1561 atgacttcat agaatgggca agtaaacatt actcaagtct tagtgaaaca gctaacgatt
     1621 taaaccgctt actcaaagat aaaaatatac agctttatcg acaatatggc ggaataagtc
     1681 caagcattga taaagataat actaatttaa aagc
//
LOCUS       DANLXF010000311         1713 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.312, whole genome shotgun sequence.
ACCESSION   DANLXF010000311 DANLXF010000000
VERSION     DANLXF010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.312"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            67..1389
                     /gene="pepP"
                     /locus_tag="PXD66_002462"
     CDS             67..1389
                     /gene="pepP"
                     /locus_tag="PXD66_002462"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HDL6320702.1"
                     /translation="MDLAYLAKMPQEEFTARRERVLEQMQDNSALLVFTESEKRRNSD
                     SEYLFRPDSYFWYLTGLAEPKSAVLLVKSEGKTESIIFVRKKDPLMETWNGRRLGIEE
                     APKTLQLNAAFDIDDIETVLAKKLENLTACYCALGIQEWGDNILFATFEKMKVNRQKV
                     PTTLIDWQPMLSEMRLIKSEFEIALIQQACHISSMAHIRAMKQARPNRYEWEIEGEIQ
                     HEFSRFGARFPAYNSIVAGGENACILHYNENDQVLKGGDLLLIDAGAEFAYYAGDITR
                     TFPINGKFSEPQKELYELTLTMQKEAINLLVPNSSIKAANDKAVQILTQGLVRLGILN
                     GDVESLIEQKAYRQFYMHGLGHWLGLDVHDVGDYGTERDRPLQIGMVLTVEPGIYIPK
                     DADVPEQYKGIGIRIEDNLLITEYGNKNLSSGCPKEIADVEQLMQSLN"
     gene            1505..1690
                     /locus_tag="PXD66_002463"
     CDS             1505..1690
                     /locus_tag="PXD66_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1508 domain-containing protein"
                     /protein_id="HDL6320703.1"
                     /translation="MYFEIYKDAKEEFRWRLKAGNHEVIATNQAYTTKQGCQKGIEAV
                     KKVTPETEVKDLTKAEA"
BASE COUNT          552 a          287 c          399 g          475 t
ORIGIN      
        1 aatttatgct gcgtttgtgc taaaataagg caggtttcga tatagatata ataaaaagga
       61 gaaataatgg atctcgctta tttggctaaa atgccacaag aagaatttac cgcacgccgt
      121 gagcgtgtgc ttgagcaaat gcaggataat tctgcgttat tggtttttac cgaatcggaa
      181 aaacgccgta atagtgactc tgaatacttg tttagacctg atagttactt ttggtattta
      241 accggtttgg cagaacctaa atcagccgta ttgctggtaa aaagcgaagg taaaacggaa
      301 agtattattt ttgtgcgtaa aaaagaccct ttaatggaaa cctggaatgg cagacgttta
      361 gggattgagg aagctccaaa aacgttacag cttaacgcag cttttgatat tgatgacatt
      421 gaaaccgttc ttgcaaaaaa attagaaaat ctgaccgctt gttattgtgc gttagggata
      481 caagagtggg gcgataatat tttgtttgca acatttgaaa aaatgaaagt caatcggcaa
      541 aaagtgccga caaccttgat tgattggcag ccaatgttat cggaaatgcg gttgattaaa
      601 tcagaatttg aaattgcctt aattcagcag gcgtgtcata tttcttcaat ggcacatatt
      661 cgagcgatga aacaagctcg cccgaatcgt tatgagtggg aaattgaggg ggaaattcaa
      721 cacgaattta gccgttttgg agcaaggttt ccggcatata attcaatcgt tgccggcggt
      781 gagaatgcct gtattcttca ctacaatgaa aatgatcagg tgcttaaagg cggcgattta
      841 ttactgattg atgcaggggc ggaatttgcg tattacgccg gcgatattac ccgaaccttc
      901 cctattaatg ggaaattctc cgaaccgcaa aaagagctgt acgaattaac cttaactatg
      961 caaaaagagg cgattaatct tcttgtgcca aacagctcga ttaaagcggc aaacgataag
     1021 gctgtacaga ttctcacgca gggcttggtt cgcttaggaa ttttaaacgg tgatgtggaa
     1081 agcctgattg agcaaaaagc ctatcgccaa ttttatatgc acggattagg gcattggcta
     1141 gggctagatg ttcacgatgt gggggattac ggcacggagc gggatcgtcc tttgcagatt
     1201 ggtatggtgt taactgttga acccggtatc tatattccta aagatgcgga tgtaccggag
     1261 caatataaag gtattgggat tcggattgaa gataatttgc tgattaccga atacggcaac
     1321 aaaaacttaa gcagcggttg ccctaaagaa attgcggatg ttgagcagtt gatgcaatcg
     1381 ttaaattaat tcgcaaattt ttgtaaaaaa atgaccgctt gctttgatat tttggggaat
     1441 ttagcttaag ctaacccgac acaaacacag tgtttgggca atcacaacac gaaggagtgt
     1501 ttatatgtat tttgaaattt acaaagatgc aaaagaagaa tttcgttggc gtttaaaagc
     1561 gggtaaccac gaagttattg caaccaacca agcttatacg actaagcaag gttgtcaaaa
     1621 aggcattgaa gcagttaaaa aagttacacc tgaaacagaa gtaaaagatt taaccaaagc
     1681 tgaagcataa tctataaagc cctgatatta ttt
//
LOCUS       DANLXF010000312         1685 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.313, whole genome shotgun sequence.
ACCESSION   DANLXF010000312 DANLXF010000000
VERSION     DANLXF010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.313"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..824
                     /locus_tag="PXD66_002464"
     CDS             <1..824
                     /locus_tag="PXD66_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HDL6320704.1"
                     /translation="SQKIYQDKQSYLVEIEKSMNDYAELVKRYEKLLKVGHSSHDEVN
                     IQKSRYFQQKSLFNELKQELIQLKSTQLSLENEIETRKTEFNNQIIRYEMQKSDLNIR
                     LMEFESVSDIIVNASLDGKIESTSVTVGQIIKENDPLAQILPQNKGNYQLVMWVPNSA
                     ISFIKQGDEVNIRYEAFPFEKFGQFKGKITSISTLPASLQELSFYKNLPVNLEQGIPL
                     YKILIELADQNVKYNDTSLYFMSGMKAEATLFLEKRKLYEWIFFPMYQLRKNMEQ"
     gene            824..1615
                     /locus_tag="PXD66_002465"
     CDS             824..1615
                     /locus_tag="PXD66_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HDL6320705.1"
                     /translation="MTGIKQLKFDFRRRIPVILQTETAECGLACLAMMLGYYDKNTNL
                     FELRSQYGTSSRGVTLHTLISIANDCGLITRPLSLELDEVPQLRLPCILHWDFNHFVV
                     LTHASEKQFIIHDPAFGKRKLSRAEFSNHFTGVALEVWSEVKFEKSNNENTISLYETL
                     KHISGIKGALVKIFALSMLIELIALLMPIGTQLVMDHVMQAKDQSLLLIICIGLFLFT
                     LFRTMVSMFRAWVSLKMNYLIDFQWTASFFSHLLKLPLDFFENAK"
BASE COUNT          560 a          278 c          295 g          552 t
ORIGIN      
        1 acagtcaaaa aatttatcaa gataagcaga gttatttagt ggaaattgaa aaaagtatga
       61 atgactatgc ggagttggtt aaacgctatg agaaattatt aaaagtaggg cattccagcc
      121 acgatgaagt gaatattcag aaaagccgtt atttccaaca aaaatcactg tttaatgagc
      181 taaagcaaga attaattcaa ttaaaatcca cccaacttag cttggaaaat gaaattgaaa
      241 cccgtaaaac cgaatttaat aatcagatta ttcgctatga aatgcaaaaa agcgatttaa
      301 acattcgttt aatggaattt gaatctgttt cagatattat tgttaatgcc tctttagatg
      361 gaaaaattga aagtaccagc gtaacagttg ggcaaattat taaagaaaat gacccgcttg
      421 cacaaattct gccacagaat aaaggaaatt atcagctagt gatgtgggta ccgaatagtg
      481 ccatttcatt tattaaacaa ggcgatgagg tgaatattcg ctatgaggct tttccgtttg
      541 aaaaattcgg gcagtttaag ggcaaaatta cgtcaatttc aaccttacca gcttctttgc
      601 aagaacttag tttttataaa aatctccctg taaatttaga gcaaggtatt ccgctttata
      661 aaattctgat tgagcttgcc gatcaaaacg taaaatataa tgatacctcc ctttatttta
      721 tgagtggaat gaaagccgaa gccacgttat ttttagaaaa acgtaaactg tatgagtgga
      781 tatttttccc tatgtatcaa ttacgcaaaa atatggagca ataatgacag gaattaagca
      841 gttaaaattt gattttcgga gaagaatacc tgttattttg caaactgaaa cagctgaatg
      901 tggcttagcc tgtttagcga tgatgttggg ctattacgat aaaaacacca atttatttga
      961 attgcgaagt caatatggta cttcctctcg tggcgtaacg ctgcatactt taatttctat
     1021 tgcaaatgac tgtggtttaa taactcgccc tctttctcta gagttagatg aagtaccaca
     1081 acttcgtctg ccttgtattc ttcattggga ttttaatcat tttgtagtat taactcacgc
     1141 aagtgaaaaa cagtttatta tccacgatcc tgctttcggt aaacgtaaat tatcccgtgc
     1201 tgaattttcc aaccatttta ccggagtcgc tttagaagtt tggtctgaag ttaaatttga
     1261 gaaaagcaac aatgaaaata ccattagttt gtatgaaacc ttaaagcata tttctggcat
     1321 aaaaggtgca ttagtgaaaa tttttgccct ttcaatgctc attgaactca ttgccttatt
     1381 gatgccaatt ggaacacagt tagtgatgga tcacgttatg caagcgaaag atcaatcatt
     1441 acttttaatt atctgtattg ggctattttt atttaccctt tttcgcacaa tggtcagtat
     1501 gtttagggct tgggtttcct tgaaaatgaa ttatttaatt gattttcaat ggacagccag
     1561 tttcttttct catctcttaa aattaccgct tgatttcttt gaaaacgcca agtaggtgat
     1621 attcattccc gatttagttc attaaaaacc attcagaaaa ctctaacttc aagcattatt
     1681 accac
//
LOCUS       DANLXF010000313         1681 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.314, whole genome shotgun sequence.
ACCESSION   DANLXF010000313 DANLXF010000000
VERSION     DANLXF010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.314"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            335..712
                     /locus_tag="PXD66_002466"
     CDS             335..712
                     /locus_tag="PXD66_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDL6320706.1"
                     /translation="MDKSQGVVSVNIRGGSGFGRANNAVGPNYMGMLAGKYQFNDSTW
                     IGALFGHSWRKISQNYKIGGGRKVTDSSIDLSKIDDLDKESSTTSPFNAAHLKQKPMS
                     RMAKLEYGDKYQTITLLPKSGSG"
     gene            1301..>1681
                     /locus_tag="PXD66_002467"
     CDS             1301..>1681
                     /locus_tag="PXD66_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005642184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDL6320707.1"
                     /translation="MTISIRQLAKKHCTYIPDEDFPNDPELGEFNCPDVNFPHTATGK
                     HKRINYSATLSAYIHDLFTPFISYSQTHRAPNIKEIFFSNLGDYGVNTALQPEQAKTT
                     QFGINGFKERVFSEEDKLGFKVLFY"
BASE COUNT          593 a          289 c          313 g          486 t
ORIGIN      
        1 tatactagac tcgtgcaata aaggcacact tctttcttca gtgtctttag cgttgcgatt
       61 taaatttaat tacatttttt ataggaaaga caatagaaat gaagatcaaa aaatatgtat
      121 ttcacctgac ttatacaaca cttatctgcc ccttacctag ggtattcgca gaggaaggtt
      181 atatgttaga tacggttgag gttaaagccc aagcaataaa aaaacagatg aagttttcat
      241 taaaacaggg gcaataagca cccgagataa aatcttggaa tcaaaccaaa gcttagagac
      301 tattgttcgt tctatgcctg gcgcatttac acaaatggat aaatctcaag gtgtggtttc
      361 tgttaatatt cgaggaggtt ctggatttgg gcgagctaat aatgcagtag gcccaaatta
      421 tatgggaatg ctcgctggca aataccaatt caatgactct acttggatag gagcattatt
      481 tgggcatagt tggcgcaaaa tctctcaaaa ttataaaata ggaggcggac gaaaagttac
      541 ggatagctcg attgacctta gtaaaattga tgatctagat aaagaaagtt ctaccacctc
      601 accgttcaat gcagctcatt taaaacaaaa accaatgagt cgtatggcta aattggaata
      661 tggcgataaa taccaaacca tcacactatt accaaagtca ggtagcgggt agacatttaa
      721 aaaaccaaaa caaccagtta aattatcgtt ttcacttgcc cgagtcacct tggttagatg
      781 ttcaagtggc ctatgcggaa aatcaaggca aacaaaacta caacaaaggg agctgaatta
      841 ataataaata tttaattcaa ggtttggcaa ttaacaacaa agcgacaacc ttaaatttga
      901 gtaacacctt taaagtgtct ccgtttaaag atggagaatg ggaagcaata ttaggtttta
      961 atcgtttaaa gaaccgctat agtaaaaata gacatcctgc ggaattaaac tataacttag
     1021 agaatggcga aaccagcgaa aacaacgatt ctgtaggaat gacagccgtt cgtaaatcgc
     1081 tttatacttc aactttccag cctgatggcg aacagaagtt cgatactttt tatctcgaca
     1141 atcactttaa ctatggtata tggggattgg atataaacgc aaatattaat accgctcggt
     1201 ttattggtca gcgttttaaa tattttcctt ttgcgattag agaattggag tcagatatta
     1261 gacaggcaag tagaaatagg cagtttgagc atttaaggtc attacaatct caattagaca
     1321 acttgcaaaa aaacattgta catatattcc agatgaggat tttcctaatg atcctgaatt
     1381 gggagagttt aactgtcctg atgtcaattt tcctcataca gcaacaggaa aacataaacg
     1441 aattaattat tcagcaacct tgtctgctta tattcacgat ttattcacgc cctttattag
     1501 ttattctcaa acacatcgag cccctaatat taaagaaata ttcttctcta atttaggtga
     1561 ctatggtgta aataccgcac tacaaccaga gcaagcgaaa acaacacagt ttggtattaa
     1621 tggattcaaa gaacgggtat ttagtgaaga agataaatta ggatttaaag tgctatttta
     1681 t
//
LOCUS       DANLXF010000314         1662 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.315, whole genome shotgun sequence.
ACCESSION   DANLXF010000314 DANLXF010000000
VERSION     DANLXF010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.315"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            24..470
                     /gene="matP"
                     /locus_tag="PXD66_002468"
     CDS             24..470
                     /gene="matP"
                     /locus_tag="PXD66_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HDL6320708.1"
                     /translation="MRYQKLITQETTWKWQYLLKKHREGENITKYEEQSLIDLKVQFL
                     STLQNSPDEVEKWIKSEMTPEQRKKMRQSVRAKRKRFFNAEKQTTKKKSIDLEYSSWL
                     RLSKYAKSQKLTLSEAIVKLTDELENKQLYLEQVAKMKSSLKDLLK"
     gene            complement(536..>1662)
                     /gene="ompA"
                     /locus_tag="PXD66_002469"
     CDS             complement(536..>1662)
                     /gene="ompA"
                     /locus_tag="PXD66_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_119863613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HDL6320709.1"
                     /translation="LVALAVLSAAAVAQAAPQANTFYAGAKAGWASFHDGLTQFDNTY
                     DYGSGINLGINLGINRNSVTYGVFGGYQILNQNNFGLATELGYDYFGRVRGNTTFSGD
                     DKEKTAAKHSAHGAHLSLKPSYEVVPNLDVYGKVGVALVRNDYYVQQHVDKDSRIKVH
                     NLKPSLLLGAGLEYAITPELAARVEYQYLNRVGNLDKAVRTTVGYLIPRGTNFQYSPD
                     IHSVSAGLSYRFGQGAAPVEAPEVVTKNFAFSSDVLFDFGKSSLKPAAATSLDAAHAE
                     ISNLGLANLAIQVNGYTDRIGKEASNLKLSQRRAETVANYIVSKGTNPANVTAVGYGE
                     ANPVTGHTCDAVKGRKALIACLAPDRRVEIQVQGSKEVTM"
BASE COUNT          535 a          317 c          318 g          492 t
ORIGIN      
        1 ctttttccta tcatattcag attatgcgat atcagaaact catcacccaa gaaacaactt
       61 ggaaatggca atatctccta aaaaaacatc gtgaaggaga aaatattact aagtacgaag
      121 aacaaagctt gatcgattta aaagttcagt tcctctctac tttgcaaaac tctccggacg
      181 aagtggaaaa atggattaag tcggaaatga cgcctgagca acgtaagaaa atgcgtcaat
      241 cagtacgcgc caaacgcaaa cgcttcttta atgctgaaaa gcaaacaacc aagaaaaaat
      301 ccattgattt agagtattcg agctggttaa ggctctctaa atatgccaaa agccaaaaac
      361 taacgctttc tgaagctatt gtcaaattaa ccgatgaact cgaaaataaa caactttact
      421 tagagcaagt tgctaaaatg aaatcaagtt taaaagattt actgaaataa aaaaagaaaa
      481 aagcccacta acaagcgggc tttttttgat tcaatttttc aactgaataa ctatattaca
      541 tagttacttc ttttgaacct tgaacttgga tttcaacacg acgatctggt gctaagcaag
      601 cgattaatgc tttacgacct ttaactgcat cacatgtgtg accagttact gggtttgctt
      661 caccgtaacc tacagctgtt acattagctg ggttagtacc tttagaaacg atgtaattag
      721 ctactgtttc agcacgacgt tgtgaaagtt ttaagttaga tgcttcttta ccgatgcggt
      781 ctgtgtaacc attaacttgg atagctaggt ttgctaagcc taagttgctg atttcagcgt
      841 gtgctgcatc taaagaagtt gctgctgctg gttttaagct tgatttaccg aaatcaaata
      901 aaacatcaga gctgaatgcg aagtttttag ttacaacttc tggtgcttca actggtgctg
      961 caccttgacc gaaacggtat gataaacctg cagatactga gtggatatct gggctgtatt
     1021 ggaaatttgt acctcttggt attagataac caactgttgt acgaactgcc ttatctaagt
     1081 taccaacacg atttaagtat tgatattcaa cacgagcagc taattcagga gtaattgcat
     1141 actcaagacc agcacctaat aatagagatg gttttaagtt gtgaacttta atgcgtgaat
     1201 ctttatctac atgttgttgt acgtagtaat cgttgcgaac taatgcaaca cctactttac
     1261 cgtaaacatc taagttagga acaacttcgt agcttggttt taagcttaag tgcgcaccgt
     1321 gagctgaatg tttagccgca gttttttctt tatcatcacc agaaaatgta gtgttaccac
     1381 gaacacgacc aaagtagtca taacctaatt ctgtcgctaa accaaagttg ttttggttta
     1441 agatttggta accaccgaat acaccgtaag ttacagagtt acggttaata ccgaggttaa
     1501 taccgaggtt aataccggat ccataatcat atgtgttgtc aaattgagtt aaaccgtcgt
     1561 ggaatgatgc ccaaccagct ttagcacctg cgtagaaagt gttagcttgt ggagctgctt
     1621 gagctactgc agctgctgat aatactgcta atgcaactaa tg
//
LOCUS       DANLXF010000315         1657 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.316, whole genome shotgun sequence.
ACCESSION   DANLXF010000315 DANLXF010000000
VERSION     DANLXF010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.316"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..223
                     /locus_tag="PXD66_002470"
     CDS             <1..223
                     /locus_tag="PXD66_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320710.1"
                     /translation="QNKNLTDESEEQAILAYLTENIMARIEKEQPLEKTLILYSEQLA
                     SFEQKSPMALHREKFEAKPTGLAELINNL"
     gene            234..1184
                     /locus_tag="PXD66_002471"
     CDS             234..1184
                     /locus_tag="PXD66_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HDL6320711.1"
                     /translation="MSLSTFAHALKLDKQGNVILFDDSPEEQAKLISRLKQNRLFKLL
                     NLVQTKHAKGEAIGLFTPIAGTTNTESGEEREPKQALAPAQKYHCEQINIDSYIRYEK
                     IDAYNLENSLEERLNQLLDDNLLRGLLMVGWQGVSRADTSNATNNQLAQDVKKGWLQK
                     IREGKPEAVINGATIGKEQQYKSLNALIKQGLGKISPAYAMGGDMIAICGRDIIADDV
                     IQLEHNDLSENLADLLTLCQQAKGGLKAVSIPYFPSNAILITRLDNLSLYLHLNNIRR
                     KAFDTPQKDSVETFYSLNVDYIVEDFNACCLIENIEVIEE"
     gene            1181..1375
                     /locus_tag="PXD66_002472"
     CDS             1181..1375
                     /locus_tag="PXD66_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320712.1"
                     /translation="MSLESYIQQAEEMELLEKLDFVSDLLELEKVSEIEAYRILTAHG
                     EPKPQPAQEKLIGCVDFLDD"
     gene            1493..>1657
                     /locus_tag="PXD66_002473"
     CDS             1493..>1657
                     /locus_tag="PXD66_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320713.1"
                     /translation="MTNYKLPARKWYTLEQATKRIKQLTGEDIEIEDLLHYWYLRKLE
                     LSIYLNRNGNG"
BASE COUNT          623 a          332 c          314 g          388 t
ORIGIN      
        1 acaaaataaa aacctcacag atgaaagtga agagcaagct attctggcat atctaacaga
       61 aaatataatg gctagaattg aaaaggaaca gccacttgaa aaaacattaa ttctttattc
      121 tgaacagtta gcaagttttg aacaaaaatc gccaatggca ttacaccgtg aaaaatttga
      181 agcaaaacct accggcttag ctgaattaat taataactta taagaggtaa aaaatgagtt
      241 tatccacttt cgcccacgca ttaaaactag ataaacaagg taacgttatt ttatttgacg
      301 atagccccga agaacaggca aaactaatta gccgcttaaa acaaaatcgc ctttttaaat
      361 tattgaattt agtgcaaacc aagcacgcaa agggcgaagc gattgggcta ttcacaccga
      421 tagccggcac aaccaacacc gaaagcggag aggaacgaga gccaaaacaa gcccttgcac
      481 ccgctcaaaa ataccactgc gaacaaatca acattgatag ctatattcgc tatgaaaaaa
      541 tcgatgctta caacctcgaa aacagcttag aagaacgctt aaatcagtta cttgatgaca
      601 accttctaag agggctttta atggttggct ggcaaggcgt aagccgtgca gatacatcaa
      661 acgcgacaaa caaccaactc gcccaagacg tgaaaaaagg ctggctacaa aaaatcagag
      721 agggcaagcc cgaagcggta attaatggag caaccatagg caaagagcaa caatacaaaa
      781 gcctaaatgc tctgattaag cagggattag gcaaaattag cccagcgtat gcaatgggcg
      841 gagatatgat cgccatttgt gggcgggata ttatcgctga tgatgtaatt cagcttgaac
      901 acaacgattt aagcgaaaac ctagccgact tattaacctt atgccaacaa gctaaaggcg
      961 gactaaaagc ggtaagtatt ccctacttcc caagcaatgc gattttaatc actcgattag
     1021 ataacctttc gctatatctt catttaaaca atatccgccg taaagcgttt gatacgccac
     1081 aaaaagacag cgtagagaca ttttactccc tcaatgtgga ttacattgta gaagatttta
     1141 acgcctgttg tttaattgaa aatattgagg ttattgaaga atgagcctag aaagctatat
     1201 ccaacaagcg gaagaaatgg agcttttaga aaaactcgat tttgtttcag acctgttaga
     1261 acttgagaaa gtcagcgaaa ttgaagccta tcgcatttta accgcccacg gcgaacctaa
     1321 accccaaccg gcacaagaaa agctaatagg ttgcgtagat tttcttgatg actaacccaa
     1381 gccccgaaag gggctttttg catttttgca acaaaaaacg accgcttgca ccctttgaaa
     1441 ttattgctat cattaataaa aaacaataac caaatcaaat gagagtacag caatgacaaa
     1501 ctataaacta ccggctagaa agtggtacac cttagagcag gcaacaaaac gcattaaaca
     1561 actcactggc gaagatatag aaatagaaga tttattacat tattggtatt taagaaagct
     1621 agaattaagt atttatttaa atagaaacgg aaatggc
//
LOCUS       DANLXF010000316         1638 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.317, whole genome shotgun sequence.
ACCESSION   DANLXF010000316 DANLXF010000000
VERSION     DANLXF010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.317"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..>1638
                     /locus_tag="PXD66_002474"
     CDS             <1..>1638
                     /locus_tag="PXD66_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005687279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDL6320714.1"
                     /translation="DLNADTSQAFLNELDQTLWTAADKLRKNLDAANYKHIVLGFIFL
                     KYISDSFTDFQAKLKTQLTTPESELYLDPALFDEQEFSQILAEELEQRDYYAAENIFW
                     VPEQARWDNIKSLSKLNLGDELPWGDKFKGVSRLIDDAFEAIERENPKLKGVLQRIAG
                     FGVPDEMLTGLIDLFSRTNFTQPMHNGEPVHLQAKDILGHVYEYFLGQFALAEGKKGG
                     QYFTPKSIVTLIVEMLEPYSGRIYDPAMGSGGFFVQADRFIQAHAGNRNAISVYGQES
                     NSTTRKLAVMNMAIRGIPFDFGDKPEDTLLNPLHIDKKMDVVMANPPFNQKEWWNESL
                     ANDPRWAYGTPPQGNANFAWLQHMIYHLSPKGKMALLLANGSMSSQTSGEGDIRKNIV
                     QADLVEAMIALPNQLFTNTQIPACIWIINKAKARKGEVLFINATQIGYLKDRVLRDFT
                     ADDIAKISDTYHNWQKQNGYENIPAFCYCATLDEIAKNDFVLTAGRYVGAVQEENDGV
                     PFAEKMQELTALLNEQFKQGRELEQQIAENLKGLGYG"
BASE COUNT          483 a          436 c          356 g          363 t
ORIGIN      
        1 tgatctcaac gctgacacaa gtcaagcctt tttaaacgaa ctcgaccaaa ccctctggac
       61 tgccgccgac aaactgcgta aaaacctcga tgccgccaac tacaaacaca tcgttcttgg
      121 ctttatcttc ctaaaataca tctccgacag ctttaccgat ttccaagcca agctaaaaac
      181 ccagcttacc acccccgaaa gcgaactcta tcttgaccct gcactatttg acgaacaaga
      241 atttagccaa attcttgccg aagagttgga acagagagat tactacgccg ctgaaaacat
      301 cttttgggtg ccggagcaag cccgctggga caacatcaaa tcattaagca aactcaatct
      361 tggcgatgaa ttgccttggg gagacaaatt taaaggtgtc agccgcttga ttgatgatgc
      421 ctttgaagcc atcgaacggg aaaaccccaa actcaaaggc gtactccaac gcattgccgg
      481 ctttggcgtg cctgatgaaa tgctcacagg cttaattgac ctgttctcac gcaccaattt
      541 cacccagcct atgcacaatg gcgaacctgt gcatctgcaa gccaaagaca ttttagggca
      601 cgtctatgaa tactttcttg ggcaatttgc ccttgccgaa ggcaaaaaag gcggtcaata
      661 cttcacgcca aaatccatcg ttaccctgat tgttgaaatg ctcgaaccct attcagggcg
      721 gatttacgac ccagctatgg gcagcggcgg cttttttgtg caagctgacc gctttattca
      781 ggctcacgca ggcaaccgca acgccatttc cgtttatggg caagaatcca actccaccac
      841 tcgcaaactg gcggtgatga atatggcgat tcgtggtatt ccctttgact ttggcgacaa
      901 gcccgaagat accctactaa accctttgca catcgacaaa aaaatggatg ttgtgatggc
      961 aaatccgccc tttaaccaaa aagagtggtg gaatgaaagc ctagcaaacg atccacgctg
     1021 ggcatacggc acaccgccgc aaggcaacgc caactttgcg tggttgcaac atatgattta
     1081 ccacctctcc cccaaaggca aaatggcact cctgctcgcc aacggctcaa tgagcagcca
     1141 aacttcaggc gaaggcgaca ttcgcaaaaa catcgtgcaa gctgaccttg tcgaagcgat
     1201 gattgccctg cccaatcagc tattcaccaa cacccaaatc cctgcctgca tttggattat
     1261 caataaagcc aaagccagaa aaggtgaagt gctgtttatc aacgccaccc aaataggcta
     1321 cctgaaggac cgcgtcttgc gtgattttac cgctgatgac atcgccaaaa tcagcgacac
     1381 ctaccacaac tggcaaaaac agaacggcta cgaaaatatc cctgcgtttt gttattgtgc
     1441 cacgctggac gaaatcgcca aaaacgattt tgtgctgacc gcagggcgat atgtcggtgc
     1501 ggtacaagaa gaaaatgacg gcgtgccgtt tgcagaaaaa atgcaggaat tgaccgcttt
     1561 attgaatgaa caatttaaac aagggcggga attggaacag caaatcgcag agaatttaaa
     1621 ggggttggga tatggcat
//
LOCUS       DANLXF010000317         1609 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.318, whole genome shotgun sequence.
ACCESSION   DANLXF010000317 DANLXF010000000
VERSION     DANLXF010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.318"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..569)
                     /locus_tag="PXD66_002475"
     CDS             complement(<1..569)
                     /locus_tag="PXD66_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDL6320715.1"
                     /translation="METLIEFFSSYGYWAVFLVLLACGFGVPIPEDITLVSGGVISGL
                     GYTNVHWMLVVSMLGVLVGDSTMYWLGRIYGEKILKFPLIRNVATPERFATVQERFEK
                     QGWKLLFMARFLPGLRAVVYLVSGITRKVSFVKFLLVDFCAAIISVPIWVYLGDYGAS
                     NLDWLKEQVANGQHLIFAILGIVILFLGW"
     gene            complement(571..1590)
                     /gene="purR"
                     /locus_tag="PXD66_002476"
     CDS             complement(571..1590)
                     /gene="purR"
                     /locus_tag="PXD66_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HDL6320716.1"
                     /translation="MATIKDVAKYAGVSTTTVSHVINKTRFVAEDTTKAVWEAIKALN
                     YSPSAVARSLKINTTKSIGMIITTSESPFFAEIVLAVEEFCYSKGYSLFLCNTQNKLE
                     KIQNHLDMLIKKRVDGILVMCSEYTANSFDIFENSNIPMVIMDWGRSDERSDIILDHG
                     VEGGYIATQHLIENGHQDIAVITGNRDKEIARTRFEGFKKALSEAGLSIREEWIQEGD
                     FEPEGGYECMNNLLKSSEKLPTAVFCFNDSMALGAISAITEKGLNVPQDISVIGYDNV
                     HSSRFYAPPLTTIHQSKSRLGSAALELLLERIEDNENAKEPKVLEFFPELVKRKSVRN
                     LNIKE"
BASE COUNT          506 a          370 c          255 g          478 t
ORIGIN      
        1 ttccagccta aaaatagaat aacaatgcct aaaatggcaa aaatgaggtg ctgcccatta
       61 gctacttgtt cttttagcca atccaaatta gatgccccat agtcgcctaa ataaacccaa
      121 attggcacag agataatagc ggcacagaaa tcaactaata aaaattttac gaagctgact
      181 tttctggtaa tacctgaaac taagtaaacc accgcgcgca agccaggtaa aaaacgagcc
      241 ataaaaagta gtttccatcc ttgtttttca aaacgttctt gcacggttgc aaaacgttca
      301 ggtgtcgcaa cattcctgat taatggaaat ttgagaattt tttcgccata gattctacct
      361 agccagtaca ttgtgctatc gccaactaat actcctaaca tactaacaac cagcatccaa
      421 tgcacattgg tgtagcctaa tcctgaaata acaccaccgg atactaacgt aatatcttca
      481 ggaatcggca caccgaaacc acaggcaagt agcacaagaa aaacagccca ataaccatag
      541 ctactaaaaa attcaatcaa tgtttccata ttactcctta atgtttaaat tccgaaccga
      601 tttacgtttg accaattcag gaaagaactc taatacttta ggctcttttg cgttttcgtt
      661 atcttcaata cgttctaata ataattctaa tgctgccgag cctaagcgag attttgactg
      721 atggattgtc gtaagaggcg gtgcataaaa gcgtgagcta tgcacgttat catacccgat
      781 cactgaaata tcttgcggca cattcagccc tttttcggta atagctgaaa ttgcccctag
      841 tgccattgag tcattaaaac aaaaaaccgc ggttggtaat ttttcactgc tcttaagcaa
      901 attattcata cactcatacc ccccctcagg ttcaaaatcg ccttcttgaa tccattcttc
      961 acgaatagac aaacccgcct cagatagcgc ttttttaaaa ccttcaaaac gggttcttgc
     1021 aatttcttta tctctattgc cggtaattac agcaatatct tgatgcccgt tttcaattaa
     1081 atgctgagtc gcaatatagc caccttcaac accgtgatct aaaataatat cactgcgttc
     1141 atcacttcgt ccccaatcca taatcaccat tgggatattg ctgttttcaa agatatcaaa
     1201 tgaattagct gtatattcgg agcacatcac caaaatgcca tcaacccgtt ttttgatgag
     1261 catatctaaa tgattttgaa ttttctcaag tttgttttga gtattgcaca aaaatagtga
     1321 ataccctttg ctatagcaaa attcctctac cgctaacaca atttcagcga aaaacggtga
     1381 ttcgctggtt gtaatgatca taccgataga tttagtcgta ttaattttca aactccgcgc
     1441 tacagcacta ggagaatagt tcagcgcttt aatagcctcc caaactgctt ttgtagtgtc
     1501 ctctgctaca aagcgggttt tgtttataac gtgagaaacc gttgtagtcg atacaccagc
     1561 atattttgcg acatctttta tcgttgccat acctttttcc tcctaaata
//
LOCUS       DANLXF010000318         1595 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.319, whole genome shotgun sequence.
ACCESSION   DANLXF010000318 DANLXF010000000
VERSION     DANLXF010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.319"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            44..>1595
                     /locus_tag="PXD66_002477"
     CDS             44..>1595
                     /locus_tag="PXD66_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HDL6320717.1"
                     /translation="MEKMMRKINQLIISPYFFLSILSASANSDFWKSDLNFNLTNITK
                     RVGVDNFTTPVAGEPWAGIGPNGEAAGTAPLYYSRIPAMQVTEDNKLVVMFDLRWNRA
                     YDQDRIDPGIAISSDGGHTWTKKTAWSFDRTNHPARRSMDPTLLHNPIDNSLYVMHGT
                     WSMSDQQWYGGRVPHFNSGSWAATIYKSTDGGLNWEKNTEFSKFSNLDVFSKVTKNGK
                     PTLGFLGGVGSGIVMQDGTLVFPIQTAHQNGIATTIMYSKDNGKTWEMPEIDNPVAPN
                     QSSLENMVFELEPGKLVMTGRGNSRWAYSSTDMGKTWELFTPTNGLLPTSSQPTQGSS
                     IYVTLPNGRKVLLISKPDGKNDGWKRGDITLWMLDAKDPSHKHKVHIIRPGSGNAAGA
                     GYSSLAYKEGNLFVAFENDGDISVKNLTEHIAEMEAKAIEWGLPDELTPALDKINALP
                     YLNKAQKETLVEKMRRANDTAIAQSFVINKEMYNLKNNSDELDKLAKNITKALPSQQN
                     IYQRALAYV"
BASE COUNT          505 a          295 c          328 g          467 t
ORIGIN      
        1 tagctgttca gctaaaaatt agtctttgca ctttatcccc ctaatggaga aaatgatgag
       61 aaaaatcaat cagttaatca tatccccata ctttttccta agtattttat ctgcttccgc
      121 taactctgat ttttggaaaa gcgaccttaa ttttaatctc acaaatatta ccaagcgtgt
      181 cggagtcgat aactttacca ctccagtagc tggcgagcct tgggctggta ttggtccaaa
      241 tggtgaagct gcaggtactg cgccattata ttatagccgt attccagcta tgcaagttac
      301 cgaagataac aaattagttg ttatgtttga tttacgctgg aatcgtgcgt atgaccaaga
      361 tcgtattgat cctggtattg cgatttcttc cgatggtggc catacttgga cgaagaaaac
      421 cgcttggtct tttgatagaa ctaatcaccc agcacgccgt tcaatggatc caacgctatt
      481 acataatcct attgataata gtttatatgt aatgcacgga acttggtcta tgtcggatca
      541 acaatggtat ggtgggcgtg ttcctcactt taatagtggc agctgggcag ctaccattta
      601 taaatctact gatggtggtt taaattggga gaaaaatact gagtttagta aattcagcaa
      661 tttagacgta ttttctaaag tcaccaaaaa tggaaaacct accttaggct ttttaggcgg
      721 ggtcggatct ggaattgtaa tgcaagatgg tacgttggtt ttcccaattc aaaccgcaca
      781 tcaaaatggt attgcgacca ctattatgta ttctaaggat aatgggaaaa cttgggaaat
      841 gccagaaatt gacaacccag ttgcaccgaa ccaaagttct ttagaaaata tggtgtttga
      901 actcgaaccg ggtaagctag tgatgactgg acgaggaaac agtcgctggg catactcttc
      961 tactgatatg ggtaagacct gggaattatt tactccaact aatggattat taccaactag
     1021 ctcacaacca actcaaggct cttctatcta tgtaacacta ccaaatggtc gaaaagtgct
     1081 actaatctct aaaccagatg gaaagaatga tgggtggaaa aggggtgata ttacactttg
     1141 gatgctagat gcaaaagatc catcgcacaa acacaaggtg catattattc gtccaggttc
     1201 aggtaatgcc gctggtgctg gttattcctc tttagcctat aaagaaggga atttatttgt
     1261 tgcttttgaa aacgatggtg atatttcagt aaaaaattta actgaacata ttgctgaaat
     1321 ggaagcaaaa gctatcgaat ggggattacc agatgaactt actcctgctt tggataaaat
     1381 caatgcactg ccttatctca ataaagctca aaaagagacg ctagtcgaaa aaatgcgtcg
     1441 tgctaatgat actgcgattg cacaatcttt tgtgattaac aaagaaatgt ataatctgaa
     1501 aaacaatagt gatgagctgg ataaattggc aaaaaatatt actaaagcac tgccgtctca
     1561 acagaatatt tatcaaagag ccttagccta tgtga
//
LOCUS       DANLXF010000319         1540 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.320, whole genome shotgun sequence.
ACCESSION   DANLXF010000319 DANLXF010000000
VERSION     DANLXF010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.320"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(196..1431)
                     /locus_tag="PXD66_002478"
     CDS             complement(196..1431)
                     /locus_tag="PXD66_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HDL6320718.1"
                     /translation="MKINRIILQSCSECGQQVRVPLTSNGNAVCPVCHNILRKSNTWS
                     LHRSAFLALAILILLPFALTFPLMSIDLLGIKINATVWDGIWKMATEGYPYTAFMVLV
                     CAVIMPIAFVLLILTIQLQRIIRQRPRYTLIFLTKIKEWVMLDVYLVALAVAAFKIRD
                     YADLQFDINLIAFVLVTLLNTLLFIKTEPKQAWERFYPEYHALPFDHPSNPTLCPTCE
                     YCFDETILDLKGRQRCPRCESNLSISDNVKIQRVWACLIAGTIMMIPANIFPISTTEL
                     AGQVSADSLMSGVLLFMEMGSYTVAAIVFIASIAVPVSKITIIFYLLLAIHYKWKHPI
                     HWQMKLLHYVHFVGRWSMLDLFVLSLMMSLLERGQILSFSVGEAAFYFGAAVFLTMFA
                     SANLDSRMLWKIHTVQKTS"
BASE COUNT          468 a          295 c          317 g          460 t
ORIGIN      
        1 gttctttagt actgcaatgg ataccctaat tgcctcaagt aaatatagta aagactattt
       61 tatatttgct aatttctttc tggctatcgg tgctacatta tcggctcttt cttactcaat
      121 agggattgag attttgggtt acgtactact tttcttactt tgcatgttat gtattattta
      181 tcggaaaatc tttatttagg atgttttctg gacagtatga atcttccaca acattctgga
      241 atctaaattt gcagaggcaa acatcgttaa gaatacggct gctccaaaat agaaagcggc
      301 ttcccctacc gagaaactga gaatctgccc tcgttcgagt agcgacatca tcaatgataa
      361 cacgaataaa tccagcattg accagcgtcc gacaaaatgg acgtagtgta gtagtttcat
      421 ttgccaatga atcgggtgtt tccacttgta atgaattgcc agcaagagat aaaaaataat
      481 ggtaattttg ctcaccggca ccgcaatact agcgataaac acaatcgctg ccacggtgta
      541 gctacccatt tccataaaca gaagcacgcc ggacattaat gaatctgccg aaacctgacc
      601 tgccagttca gttgtcgaaa tcgggaaaat atttgccgga atcatcataa tagtccctgc
      661 aattaagcaa gcccaaaccc gttgtatttt tacgttgtcg gaaatagata aattgctttc
      721 acaacgaggg cagcgttgcc gtccttttaa gtctaaaata gtttcatcaa agcaatattc
      781 acaggtcggg caaagtgttg gattgcttgg gtgatcaaac ggcaaggcgt gatattctgg
      841 gtaaaaacgt tcccatgctt gttttggctc ggttttaata aaaagtaggg tgtttaagag
      901 tgttaccaac acaaaggcga ttaagttgat atcgaattgt agatcggcat aatctcgaat
      961 tttaaaagcg gcaaccgcaa gagcaactaa atagacatca agcattaccc attcttttat
     1021 tttagttaag aaaatcagcg tgtaacgggg gcgttggcga ataatacgtt gtagctgaat
     1081 ggttaaaatt agcaatacaa atgcaatcgg cattattacc gcgcagacta gcaccataaa
     1141 agcggtgtaa gggtagccct ccgttgccat cttccaaata ccgtcccata cggtagcatt
     1201 aattttgatg ccgagtaagt caatgctcat caagggaaaa gtaagtgcga agggaagcaa
     1261 aattaaaatg gctaatgcca aaaaagcact acggtgcaag ctccaagtat tgcttttgcg
     1321 taatatatta tggcagaccg ggcaaacggc attgccattt gacgtgagcg ggactcgcac
     1381 ttgttgccca cattcgctac agctttgtaa aatgattctg tttattttca ttttttgatc
     1441 ttctattaaa ttaggtggtt attttctact ttatcgtaga gattgcaagt ggttaaattt
     1501 ggcaaaattt ttgcaagttg cattttatgc aatatgaaat
//
LOCUS       DANLXF010000320         1535 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.321, whole genome shotgun sequence.
ACCESSION   DANLXF010000320 DANLXF010000000
VERSION     DANLXF010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.321"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            40..1404
                     /gene="glmU"
                     /locus_tag="PXD66_002479"
     CDS             40..1404
                     /gene="glmU"
                     /locus_tag="PXD66_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDL6320719.1"
                     /translation="MTQLSVVILAAGKGTRMYSDLPKVLHPVAGKPMVKHVIDTAKQL
                     DAQQIHLIYGHGADLLKARLSEEPVNWVFQAEQLGTGHAMQQAAPFFADDENIVMLYG
                     DAPLITKATLERLIAAKPENGIALLTVELENPTGYGRIIRENGSVVAIVEQKDANEAQ
                     LKVKEVNTGVMVASGASFKKWLKNLNNNNAQGEYYITDVIAMANQEGFKVQAVQATEF
                     MEVEGANNRLQLAALERFYQKREAEKLLLAGVTIIDPSRFDLRGTVTHGKDVQIDVNV
                     ILEGEIKLGNNVKIGAGCVLKNCEIGDNVEIKPYSVIEDSIVGAKSAIGPFSRLRPGA
                     ELAEETHIGNFVEIKKATIGKGSKVNHLTYVGDAEIGKECNIGAGVITCNYDGANKFK
                     TIIGDNVFVGSDSQLIAPVTIASGSTIGAGATVTKDIAENELVISRVPQRHIQGWQRP
                     TKKK"
BASE COUNT          511 a          306 c          334 g          384 t
ORIGIN      
        1 ctataagcct tcatctattt agtcaaataa ggattctata tgactcaact gagcgttgtt
       61 attttagctg ccggtaaagg cactcgtatg tattcagatt tacccaaagt gttacaccca
      121 gttgccggta aacccatggt aaaacacgtt attgatactg ccaaacagct agatgcccag
      181 caaattcatt taatttacgg acacggtgcc gatctgttaa aagcacgttt aagcgaagag
      241 cctgtaaact gggtattcca agccgaacaa ttaggcacag gacatgcaat gcaacaagca
      301 gctccttttt tcgctgatga tgaaaacatt gtaatgcttt atggtgatgc ccccctaatc
      361 acaaaagcca ctttagagcg tttaatcgca gccaaaccgg aaaatggtat tgcattacta
      421 accgttgaat tggaaaaccc gacaggctac ggacgaatta ttcgtgaaaa tggttctgtg
      481 gtagcgattg ttgagcaaaa agatgccaat gaagcacagc ttaaagtcaa agaagtgaat
      541 accggcgtga tggtagcaag cggtgcgagc ttcaaaaaat ggttgaaaaa cctaaataac
      601 aacaatgctc aaggtgaata ttacatcact gatgtgatcg caatggcaaa ccaagaaggc
      661 tttaaagtac aagcggttca ggcaactgaa tttatggaag tagaaggggc aaacaaccgt
      721 ttgcaacttg ctgcacttga acggttctac caaaaacgtg aggcggaaaa actgttattg
      781 gctggtgtaa ctattatcga cccaagccgt tttgatcttc gtggcactgt cacccacgga
      841 aaagatgttc agatcgatgt gaatgtcatt ctagaaggtg aaatcaaact cggtaacaat
      901 gtaaaaattg gtgcaggctg cgtactgaaa aactgcgaaa ttggcgataa tgttgaaatt
      961 aaaccttatt ctgtgattga agattcgatt gtcggggcaa aatccgctat cggtccattc
     1021 tctcgcttac gcccgggggc agaattagcg gaagaaaccc atatcggtaa ctttgtggaa
     1081 atcaaaaaag ctaccatcgg caaaggttca aaagtaaatc atctcactta tgtaggcgat
     1141 gcggaaatcg gcaaagaatg taacatcgga gcaggtgtaa tcacttgtaa ctatgatggt
     1201 gcgaataaat tcaaaaccat tattggcgat aacgtatttg tcggctcaga cagccaactc
     1261 attgccccgg tcacgattgc cagcggttca accattggtg ccggtgcaac agtcactaaa
     1321 gacattgcgg aaaatgaact ggtaattagc cgtgtaccac aacgccatat tcaaggctgg
     1381 caacgcccga ctaaaaagaa atagtgccag tggtcaaaag ttattatatt taaaaattgg
     1441 ctttgccaat aacagcaaag ccatacactt taaaaaggat aatgaacaat atatttaaaa
     1501 caaaacccct aacaataaat attgttaggg gttta
//
LOCUS       DANLXF010000321         1531 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.322, whole genome shotgun sequence.
ACCESSION   DANLXF010000321 DANLXF010000000
VERSION     DANLXF010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.322"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..259
                     /locus_tag="PXD66_002480"
     CDS             <1..259
                     /locus_tag="PXD66_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL6320720.1"
                     /translation="TYRSYDALDVSEFHHERINHSELFAEKQNHINGIENFWNQAKRV
                     LRKYNGINRKNFPLFLKECEFRFNFGTPKEQLKTLRKWCEI"
     gene            398..1450
                     /locus_tag="PXD66_002481"
     CDS             398..1450
                     /locus_tag="PXD66_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320721.1"
                     /translation="MKFNKKLILTFAATLVLSACGGSGSGGSSSTPNHPKPVLVPKTQ
                     NNLQAQNVPQAQNASQAQNAPQAQNAPQAQNAPQVENAPQVENAPQAEVTPPVPQPQS
                     QKIDGSFDKIGSVKLNKEAQTLELSRFTLVDKLGTPPKFDKVSGKKIIEEKDFLVLNL
                     SDINAEQLSGDFLIRRSDDLFYGYYHDTNGKNLVDAADKFSQYFVVYDEKRVNDNISD
                     KLTATYRKKEGFVYGSNPHTKEFAARISKLGDVEIKFENGQAQGSIKDEKDGNAEIFT
                     IKGDTKQLEITPTESNRIIIAILDQNQKSYTPGMEKAIMETKFIDSKAGNSDQKYLIG
                     EAKSDNWQAIMVSEKK"
BASE COUNT          564 a          277 c          280 g          410 t
ORIGIN      
        1 tacctatcgt agctatgatg cccttgatgt gagcgaattt catcacgagc gaatcaacca
       61 ttccgaactg tttgcggaga aacaaaacca tatcaatggc attgaaaatt tttggaatca
      121 agcgaagcga gtactccgaa aatataacgg aattaaccga aaaaatttcc ctttattctt
      181 gaaggaatgt gaatttcggt ttaactttgg tacaccaaaa gaacagctca aaacgttgcg
      241 aaaatggtgt gagatttagg gctaatctac tacagcccca aaaattttca taagggaaac
      301 gtttacgtaa aactcctcag accactcatt cttattttat ataaaaaatg tgatagactt
      361 ctcgcagttt cgttttatat atttaaggaa taactaagtg aaattcaata aaaaattaat
      421 tttaacattt gctgcaacct tagttttaag tgcttgcgga ggaagcggta gcggaggttc
      481 gtcttcaaca ccgaatcacc ccaaaccagt actagtacca aaaacacaaa ataatcttca
      541 agcacaaaat gttcctcagg cacaaaatgc ctctcaggca caaaatgccc ctcaggcaca
      601 aaatgctcct caggcacaaa atgctcctca ggtggaaaat gctcctcagg tagaaaatgc
      661 tcctcaagca gaggttactc cgcctgtacc acagccacaa tcacaaaaaa ttgacggttc
      721 ttttgataaa attggttcag taaaactcaa taaagaggct caaactcttg agcttagtag
      781 attcactttg gtggataaat taggcacacc accgaagttt gataaagtaa gcggtaaaaa
      841 aattattgaa gaaaaagatt ttctcgtatt aaatttgtct gatattaatg ctgaacaact
      901 ctctggcgat tttcttattc gccgtagcga tgatctattc tatggctact atcacgatac
      961 aaatggcaaa aatcttgtcg atgctgccga taaattcagt caatattttg tcgtgtatga
     1021 tgagaaacgg gtaaatgata atatctctga taaattaaca gcaacttacc gtaaaaaaga
     1081 aggctttgta tatggttcaa atccacatac taaagaattt gccgcacgga tcagcaaatt
     1141 gggggatgta gaaattaaat ttgaaaatgg tcaagctcaa ggaagtataa aagacgaaaa
     1201 agatggaaat gctgagatct ttactattaa aggtgataca aaacagttag agattacccc
     1261 aacggaaagt aaccgaatca ttatagcaat tttagaccaa aatcaaaaaa gctatactcc
     1321 aggaatggaa aaagcaatta tggaaactaa gtttattgat tcaaaggctg gtaattccga
     1381 ccaaaaatac ttaatcggtg aagcaaaaag cgataactgg caagcaataa tggttagcga
     1441 gaaaaaataa agttatcttt tgctaaaaac tgaaataaaa aggctgagtc cgggtaatat
     1501 cggcctcagt cttttaaatt gtagaaaatc a
//
LOCUS       DANLXF010000322         1496 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.323, whole genome shotgun sequence.
ACCESSION   DANLXF010000322 DANLXF010000000
VERSION     DANLXF010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.323"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            240..719
                     /locus_tag="PXD66_002482"
     CDS             240..719
                     /locus_tag="PXD66_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HDL6320722.1"
                     /translation="MARVTTELNNTQIKNAKAQTKDYKLMDGKGLFLLVKKNGSKLWR
                     FRYKKPITGKESDLGIGSYPETTLAQARTIREEYRALLAENIDPLEHRQRLEAEALAE
                     IENTFYKVCLKWRDKCESRVKNGELQALTLKKNWRILEKYLLPCRLLYSKHHAKTSY"
     gene            688..1452
                     /locus_tag="PXD66_002483"
     CDS             688..1452
                     /locus_tag="PXD66_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005704989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL6320723.1"
                     /translation="MPNITPKLVINALEPAKEKGIGDTLKRAIRLLNEVMNFAVNADV
                     IEFNKCQNVGSNFSVAKSEHNPTIHPKELPQFLAALRDYNLSFQTKMLIKWQLLTMTR
                     SREASETLWSEIDLEAKTWTISAERMKMKRPHVIPLSRQAVEILERMRKITGNSPFVF
                     QSEMKPRQPMNSQTANRAIGLLGYAGTLTAHGMRSIASTYLNEQLVNYDVVEACLAHV
                     IKDQTRKAYNRSDYLDQRVDVMQQWADYVESCSTGI"
BASE COUNT          497 a          297 c          308 g          394 t
ORIGIN      
        1 gaccaactta aattttccac tcttatttct tcattcgcat tcttacgcaa aaccattgat
       61 gatactggct ttagagcaat tttaaattat ttcttcattc gtattctttc gtgttcttgc
      121 gtttacatcc ccttatttta agggtatatt tgagggtata acgtaaaagg gtatgaaacc
      181 cataccctta aatcccttat gattctgata aagcacaatg cgattttagg taaattttta
      241 tggctagagt taccacagaa ttaaataaca ctcaaatcaa aaacgcaaag gctcaaacca
      301 aagactacaa gctaatggac ggcaaggggc tgtttttgct ggttaagaaa aacggctcga
      361 agttatggcg gtttaggtat aaaaagccga ttaccggcaa agagagcgat ttaggcatag
      421 gcagttaccc cgaaactacc ctagcccaag caagaacgat tagagaagaa tatagggcat
      481 tactagcgga aaatatcgac ccactcgaac accgccaaag attagaggct gaagcgttag
      541 ccgaaataga aaacaccttt tataaagtct gcttgaaatg gcgtgataaa tgcgaaagcc
      601 gtgtaaaaaa tggcgaactt caagccttaa cgctgaagaa gaattggcga atccttgaaa
      661 agtatctttt gccttgccga ctactctatt ccaaacatca cgccaaaact agttattaat
      721 gctttagagc ctgctaaaga aaaaggcata ggcgacaccc ttaaacgagc aattaggcta
      781 cttaatgaag tgatgaattt tgcggtaaat gcggacgtta tcgagtttaa caaatgccaa
      841 aatgtaggct caaattttag cgtagcgaaa tctgaacata acccaacgat acacccaaaa
      901 gagctacctc aatttttagc tgcattgcgt gattacaacc tatcatttca aactaagatg
      961 ttgattaaat ggcaactatt aacaatgaca cgctcacgag aggcaagcga aacgttgtgg
     1021 agtgaaattg atttagaggc gaaaacgtgg accatttcag ccgaaagaat gaagatgaaa
     1081 cgccctcacg ttatccctct ttcacggcaa gcggtcgaaa ttttagaaag aatgcgaaaa
     1141 atcacaggga atagcccatt tgtttttcaa agcgaaatga aaccacgcca accgatgaac
     1201 agccaaaccg ccaacagagc cataggctta ttaggctatg ccggcactct aacggctcac
     1261 ggtatgcggt ctattgcctc aacctatttg aatgagcagt tagtcaatta tgatgtagta
     1321 gaggcttgtt tagctcacgt tatcaaagac caaacccgaa aggcttataa ccgttctgat
     1381 tatttagatc agcgtgtaga tgtaatgcag caatgggcgg attatgtaga aagctgctca
     1441 acggggattt agtaaatttt tggcggaatt tgaccgcttg tttaattaga ggtgta
//
LOCUS       DANLXF010000323         1492 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.324, whole genome shotgun sequence.
ACCESSION   DANLXF010000323 DANLXF010000000
VERSION     DANLXF010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.324"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            24..>1492
                     /locus_tag="PXD66_002484"
     CDS             24..>1492
                     /locus_tag="PXD66_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HDL6320724.1"
                     /translation="MLSKLQRFTLMIKSTIIKHPLEILFVTYVTILLINNVSDYELIK
                     RFQNLIALFPICFILLYLIRDTKAYWLFAPIPIAIVFFFGQINLTLLEDSRYWATIFC
                     VFLCLISQYWYKNNHYFVAGTTNKLVNIVFATVLSGIIFATLSAITIAIIALFDLNKQ
                     NNFHIFEKFGIFSSFWAFPVLFLTLEQQTTNDSRYLNRISEMLLNWILSPALIIYTAI
                     IYAYVIFIAAKGQMPDGVVANVALPYLAFGLAIQAVQLLLKNAKWQWFYSHFTYLALL
                     PLTLIWYAIYIRFSHYGLTEARIYLVIGTITLSICYTLLLFKQLAQYRLFSAVSIAII
                     LISVFILDPKTVALKDQTKRLDNYLLKHKLLDVNNKISTKAVLKYQESLNENRDEAEY
                     FYSMLHYVARGMPAAQFKEKYGVNSSYDLMSEIRERNTDPRYFEATNSELVRLTKADM
                     ANAREYITFSSQLYRPNSFSHQKIENKLCQKRLSQGYYF"
BASE COUNT          467 a          267 c          228 g          530 t
ORIGIN      
        1 ttttcatagg caggagatct attatgctaa gtaagctaca acgctttacc ttaatgataa
       61 aaagcaccat cattaagcat ccactggaaa ttttattcgt tacttacgtc acgattttac
      121 ttatcaataa tgtgagcgac tacgaattaa tcaaacgctt tcaaaatcta attgctcttt
      181 ttcctatttg ctttattttg ctttacctga tacgcgatac caaagcttat tggctattcg
      241 ctccgattcc gatagcaatc gtctttttct ttggtcaaat taatttaaca ttattggaag
      301 attctcgtta ttgggctacc atattttgtg tattcctttg tttgatcagt caatattggt
      361 acaaaaacaa ccattatttt gtagccggaa caactaataa attagttaac attgtctttg
      421 ctacagtttt atctggcatt atttttgcaa cattaagcgc tattaccata gccattattg
      481 ctttatttga tttaaataaa caaaacaact ttcatatttt tgaaaaattt ggcatttttt
      541 ctagtttttg ggcattccct gttttatttt taactcttga acaacagact accaatgatt
      601 ctagatatct aaaccgcatt agtgaaatgt tactaaactg gattttgtct ccggcattaa
      661 ttatctatac tgcaattatt tatgcttatg taatttttat tgcggctaag gggcaaatgc
      721 cagatggggt tgttgcaaac gtagcgcttc cctatttagc tttcggccta gctatacaag
      781 cggtgcaatt attactaaaa aatgcaaaat ggcagtggtt ttattctcac tttacctatt
      841 tagcactttt gccgttaaca ttaatttggt atgccatcta tattcgtttc agccattatg
      901 gcttaactga agcccgtatt tatttagtga ttggcacaat aaccttatct atttgctata
      961 ccttattact atttaaacag ttggcgcaat atcgtctctt ttctgcagtg tcgatagcaa
     1021 ttattttaat ttcagtgttt attttagatc cgaaaacagt tgcgcttaaa gatcaaacca
     1081 agcggttgga caactatttg ctaaaacaca aattattaga tgtaaataac aaaatcagca
     1141 caaaagctgt acttaaatac caagaatcat taaatgaaaa ccgagatgaa gccgaatatt
     1201 tttattcaat gctccactat gttgccagag gaatgcctgc tgcacaattt aaagagaaat
     1261 atggcgtaaa ttcaagctac gatttaatga gtgaaataag agaaagaaat actgacccac
     1321 gttattttga agctactaat tctgagctgg ttcgcttaac taaagcagat atggcaaatg
     1381 ctcgtgaata tattacattt agtagccaat tatatcggcc taattcattt tcccatcaaa
     1441 aaatagaaaa taagctatgc caaaaacgcc tttctcaagg ttactatttt ga
//
LOCUS       DANLXF010000324         1480 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.325, whole genome shotgun sequence.
ACCESSION   DANLXF010000324 DANLXF010000000
VERSION     DANLXF010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.325"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            50..373
                     /locus_tag="PXD66_002485"
     CDS             50..373
                     /locus_tag="PXD66_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL6320725.1"
                     /translation="MKLSSELQNQINQLNQLRKQALKQEQDEQKLFEKNKIEDLPHFG
                     TQLNAQRKLLGIELTTLEMQTGISSSTLKRLFKDPSQVKFQTICIVAETLGFNLCAIN
                     THKQE"
     gene            complement(450..1468)
                     /gene="glpX"
                     /locus_tag="PXD66_002486"
                     /pseudo
     CDS             complement(450..1468)
                     /gene="glpX"
                     /locus_tag="PXD66_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484632.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
BASE COUNT          457 a          354 c          261 g          408 t
ORIGIN      
        1 tatttaacct atgaacacat aaataatcta cttttagctc aaaaataaaa tgaaactatc
       61 aagcgaacta caaaatcaga taaaccaact aaatcagtta cgaaaacagg cactcaaaca
      121 agagcaagac gagcagaaat tgtttgaaaa aaataaaatt gaagatttgc cacattttgg
      181 cacgcagctt aatgctcaga ggaaactatt aggcatagaa ttaaccaccc ttgaaatgca
      241 aaccggcatt tcaagctcta ccctaaaacg cttatttaaa gacccaagcc aagtgaaatt
      301 tcagacaatt tgtattgtgg cagaaacact cggctttaat ttatgtgcaa ttaatactca
      361 caaacaagag taagcgtgac aatgtagtga ttttgattac tttgggaaga caaaaaatcg
      421 ggctaaaagc ccgatttatc agagaaaagc tataaatcca ataaagaaag gagttcgtta
      481 tctttacgat cgacataatg gtcggattga attttacgaa tggttctaga tttgccacga
      541 ataagtaagg tttcagtgct ggcaatattg cctctacgtt taatcccttt taacaattca
      601 cctgaagtaa tgccggttgc aacaaagacg atattgtcat cacgcaccaa atcttctaat
      661 ttcaacaccg tattcaccgg tacattcatt tcattacagc gtaaaagctc tttttcggta
      721 gcagttaaat tttccgggct attccccttc acttggttgc gaggaattaa acgtgcttgc
      781 atatcgccac ccaatgcacg aaccgcagcc cccgccacta ccccttcagg tgctccgccg
      841 ataccataaa gcagatccaa ttcgctctct ggcaaacagc attgcaccgc agccgcaaca
      901 tcgccatcag gaatcgcaat cactcttacc cctaattttt gcacatcggc aattattttt
      961 tcgtggcgag gcttagcaag atagcaatgg ttaattggga aagtaatttg ccctttttcg
     1021 aggcgacacg gcgaagattt tgttcaagcg gaagatttaa atcaatcatt cctttgcatt
     1081 cctgccccac cactaatttt tccatataca tatcaggggc ttttaaaaag gtttctttgc
     1141 cgcctgctgc taataccgac aacgcatttg cctgccccat tgcagtcata cgagtaccgt
     1201 caatcgggtc aaccgcaata ctgatttcct catcttctag gcgggataga ccgattttct
     1261 cgccaatata aagcatcggt gcttcgtcaa tttcgccctc accaattacc acttcgccac
     1321 ggatttccat ttggttcagc ataaaccgca ttgctttcac cgcagcttca tcagcggcat
     1381 ttttatcccc acggcctagc caagagtaag ctgctagagc cgctgcttcc gtaacacgag
     1441 aaaattcaaa agaaagagtt cgtttcattt ttgttcctta
//
LOCUS       DANLXF010000325         1476 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.326, whole genome shotgun sequence.
ACCESSION   DANLXF010000325 DANLXF010000000
VERSION     DANLXF010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.326"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            40..>1476
                     /gene="mltF"
                     /locus_tag="PXD66_002487"
     CDS             40..>1476
                     /gene="mltF"
                     /locus_tag="PXD66_002487"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HDL6320726.1"
                     /translation="MKGLIARLFIAVALLLWAWDMIFPWQKIIHSEANHYTQIQQSKT
                     LKVGMINHPLSYFVGPEGKSGIEYDLATAFAQYLKVNLDIKTYDSSEKLFEALRNNDV
                     DIAAAGLLYHSELNDEFQIGPSYYSASWQLVYKKGSARPYRLSDLRENVIIPAGSAVI
                     PILNQLKVDNPELKWQISDKFTQEELLLKVAQGEISHTIAMSVDISAAQHINPNLAVG
                     FDLTDENPVLWYLANSSYSELQSVLLDFMSYSMETGLISRIEEKYFNHLAKFDYVDIQ
                     SYLNAIKTILPKYEPLFRAHKGELEWQMLAAIAYQESHWDPNATSPTGVRGIMMLTKD
                     TADRMKISDRTNPAQSIKAGSEYLHMLMRQMPETIPQADRIWYSLAAYNMGLGHLLDL
                     RRLTKQLGGDPDNWLDVKKNLPLLAEKRYYANLKYGYARGYEALQYVENIRRYYGSII
                     NYQRVEEQKMQEQENLPDGQIKQENSSYE"
BASE COUNT          481 a          229 c          307 g          459 t
ORIGIN      
        1 taggcttaat gcttagcttg aaataatgag gattttaaat tgaaaggatt aattgctcgc
       61 ctttttattg ccgtagcatt gttactgtgg gcttgggata tgatttttcc ttggcaaaaa
      121 atcattcatt cagaagcaaa tcattataca caaattcagc aaagcaaaac gttaaaggtt
      181 ggaatgatca accatccact ttcttacttt gttggtcctg aaggaaaaag tggcattgaa
      241 tatgatttag caacagcctt tgctcaatat ttaaaggtca atcttgatat taaaacttat
      301 gatagtagcg agaaattgtt tgaggcctta agaaataatg atgttgatat tgctgccgct
      361 gggctgcttt accattctga attaaacgat gaatttcaga taggtccttc ctattattcg
      421 gcttcttggc agcttgttta taaaaaggga agtgctagac catatcgact ttccgatttg
      481 cgggaaaatg tgattattcc agccggttct gcggtaattc caatattaaa tcagcttaag
      541 gtggataatc cggaactaaa atggcagatc tcggataaat ttacacaaga agagctattg
      601 ttaaaagtcg cacaaggtga aatttcacat acaattgcaa tgtcggttga tatttctgct
      661 gcacagcata ttaatcctaa tttagccgta gggtttgatt taactgatga aaacccagtg
      721 ttatggtatt tagcaaatag ttcttatagt gaactgcaat ctgtgctgct ggattttatg
      781 tcttattcta tggaaactgg gctgatatca agaatagaag agaaatattt taatcattta
      841 gctaagtttg attatgttga tattcagagc tatttgaatg caattaaaac tattttacct
      901 aaatatgaac ccctttttcg ggcacataaa ggtgaattag aatggcaaat gctggcggct
      961 atcgcctatc aggagtctca ttgggatcct aatgcaactt ctccaaccgg agtgagggga
     1021 attatgatgc tgacaaaaga tacggcagat agaatgaaaa tttctgatag gactaaccct
     1081 gcacaaagta tcaaagcggg atctgaatat ttacatatgc taatgagaca aatgccggaa
     1141 actattcctc aagcagatag aatttggtat agcctagcag cgtataatat gggattgggg
     1201 cacttgttgg atcttcgtcg attaacaaaa caacttggtg gtgatccgga taattggctt
     1261 gatgttaaaa agaatctgcc attgcttgcg gaaaaacgtt attatgctaa tctaaaatac
     1321 ggctatgctc gaggatatga agcattacag tatgttgaaa atatacggcg ttattatggt
     1381 agtattatta attatcaacg agttgaagag caaaagatgc aagaacaaga aaatttacca
     1441 gatggtcaaa taaaacagga gaattcttct tatgag
//
LOCUS       DANLXF010000326         1472 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.327, whole genome shotgun sequence.
ACCESSION   DANLXF010000326 DANLXF010000000
VERSION     DANLXF010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.327"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..381)
                     /locus_tag="PXD66_002488"
     CDS             complement(<1..381)
                     /locus_tag="PXD66_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL6320727.1"
                     /translation="MSRKYKFHEKSGAYFVSFATVYWVGVFVREIYFQVIVDALDYCR
                     KHKGMILYAYCIMPTHIHLLFQAENQNPSDLIRDFKTFTAKILIQRIKDNLQENRREW
                     LLWMFSRAAERRCNVKQYQFWQHHN"
     gene            complement(985..>1472)
                     /locus_tag="PXD66_002489"
     CDS             complement(985..>1472)
                     /locus_tag="PXD66_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transferrin-binding protein"
                     /protein_id="HDL6320728.1"
                     /translation="SAPSLFIFHKNYEKPVNIKGIELEFSYDIGSFYGNLAYARQNTN
                     QPVSFTDASSRVDAASHYGFKTQGFGASKISALPRDYGSLELGTRWFDQRLQIGGVAK
                     YYGKSKRIAIDEFNYTYYPGTHIVKETHRLEETVNKQPIIVDFYVSYEPIKSLILKLE
                     L"
BASE COUNT          491 a          278 c          228 g          475 t
ORIGIN      
        1 gttatgatgt tgccagaatt gatattgttt tacattacaa cgtcgttcag ccgcccgaga
       61 aaacatccac agcaaccatt cccgacggtt ttcttgcaaa ttatctttaa ttctctgaat
      121 aagtatttta gcggtgaatg ttttgaaatc tcgtattaaa tcagaaggat tttgattttc
      181 tgcttgaaat aataaatgga tatgagttgg cataatacaa taggcataca aaatcattcc
      241 cttatgtttt ctgcagtaat ctaaggcatc aacaattacc tgaaaataaa tctctctcac
      301 aaacacacca acccaataaa cagttgcaaa actgacaaaa taagcaccgc tcttttcgtg
      361 gaatttatat tttcggctca tagcctttct caacttaaaa aggaataaga aaaataccct
      421 attcaaaatt aaattccaca agccaatata actttttcga tctagatcgc aaaaatcatt
      481 aaaacttgca aagtgttgat aaaatttaac cgcttgtgat aagccaaaga aatactgaaa
      541 tgatagcaat ggatggataa ataaaatgat gacgcaagcg tccacgcttg tgccaaaaac
      601 aaaagcccaa ataaaccaaa ttttacttgg ctgatttagg cttttatctt atgactgaaa
      661 tactttcaga acaggaatta actggcacaa gcgtggacgc ttgcaccatc tgggttaaat
      721 ccttatatta taaggtttta acctactgtg cagactcaat tttagtcact atgccaaata
      781 attagttaaa ggaaatttaa gctagcttat ctaggacaac cccatttaga atttataact
      841 tgtgctaaac actatagttc tccctcttgc ataattattg agcgtataaa taggttcatc
      901 actgatatca gaattgtaaa ccctctgact tgctgcatca ttattagcat caagaggatc
      961 aatgtacttt ttgtcaaaga cattttatag ttctaacttt aatataagcg acttgattgg
     1021 ttcgtagctc acgtaaaaat caacaataat aggttgttta tttacggttt cttcaagccg
     1081 atgagtttct ttcacaatat gtgttccagg ataataagta tagttaaact catctatcgc
     1141 aatacgttta ctttttccgt aatattttgc cactccgcca atttgtagtc tttgatcaaa
     1201 ccatcttgtc cctaactcca atgagccata gtctcttggt aacgctgaaa ttttgctggc
     1261 accaaaacct tgagttttaa aaccataatg agaagcggca tctacccttg aacttgcatc
     1321 tgtaaaggat acgggttgat tcgtattttg acgagcataa gctaagttgc catagaaaga
     1381 gcctatatca taactaaatt ctaattctat ccctttgata tttactggtt tttcatagtt
     1441 tttgtggaaa atgaataaac taggagcact ac
//
LOCUS       DANLXF010000327         1450 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.328, whole genome shotgun sequence.
ACCESSION   DANLXF010000327 DANLXF010000000
VERSION     DANLXF010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.328"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            89..1408
                     /locus_tag="PXD66_002490"
     CDS             89..1408
                     /locus_tag="PXD66_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDL6320729.1"
                     /translation="MNHLLYSVEDKPPFGLSLLLASQHLLAALGGIIAVPLVLGNVLK
                     LSTADTIILVNAALLISGIVTIIQCRGIGPIGIRLPSVMGTSFTFVAAALAIGFSESG
                     IAGIMGASLVGSLVMIIGSFFMPYIRKLFPPVVTGTVIMMIGLSLIPVAVDWFAGGQR
                     TDPHYATPENLIMASFVLIIVVALVQWGKGIFSAAAIVIGMMIGYIVSLALGWVSFNG
                     VKEAQLFALPQPLHFGLEFPISGIIGMSIAYLVTIVESSGNFLALGNATKTEITGKHL
                     RGGVLCDGLGSAFAAIMSTTPFSSFSQNIGVISLTGVASRYVVAFTGALLVLAGLFPV
                     FGALIVSIPLPVLGGAGLMMFAMIIAAGIQMLDNIQRSKRNGLIIAISIGCGLAVTTR
                     PELLDKLPHFFKEVFGSGITVGSLLALILNLVLPEDKITKKENKSDE"
BASE COUNT          388 a          278 c          296 g          488 t
ORIGIN      
        1 ctcttatata aaaacaatta cgcaaacgat tgcttattaa aaaaacttca gtataatgcc
       61 gacatttttt aactctcgag gaatcactat gaaccattta ctttattctg tcgaagacaa
      121 accgcctttc gggctgagct tattgcttgc ctcacaacat ttattagccg ctttaggtgg
      181 cattatcgct gtacctttag tgctagggaa tgtactcaaa ctctctactg cggatactat
      241 aatcttagtt aatgctgcac ttttaatttc aggcatcgtc actattattc aatgtcgagg
      301 tattggccca atcgggatta gactaccgag tgtaatggga actagcttta cgtttgttgc
      361 agcagcatta gcgattggtt tcagtgaaag tggcattgcc ggcattatgg gagcctcttt
      421 agttggctct ctagtgatga ttatcggcag tttttttatg ccttatatcc gtaagctttt
      481 tccacccgtg gtaacaggta ctgtcataat gatgattggc ctaagtttaa ttcctgttgc
      541 agtagactgg tttgctggtg gtcagcgtac tgatccacac tatgcaaccc cagaaaatct
      601 tataatggct agttttgtat taattattgt cgttgcttta gtacaatggg ggaaaggtat
      661 tttttcagct gctgctattg tgattggtat gatgataggt tatatcgttt cgctagctct
      721 gggttgggta agttttaatg gcgtgaaaga agcacaactt tttgcgttac cacaacctct
      781 acattttggc ttagaatttc caatctccgg tatcatcgga atgtcaattg cttatttagt
      841 taccattgta gaatctagcg gaaatttctt agcactaggc aatgcaacta aaactgaaat
      901 taccggaaaa cacttacgtg gaggtgtatt atgtgatggt ttaggctcag catttgcggc
      961 aattatgtcc acaactccct tttcttcttt ctcacaaaat attggggtca tttcattaac
     1021 cggtgtagcc agtcgctatg ttgtagcatt tacaggcgca ttacttgtac tagccggttt
     1081 atttcctgta tttggagcat taatcgtgtc tatcccgtta cctgtgcttg gcggtgcagg
     1141 cttaatgatg tttgccatga ttatcgcagc cggtatccaa atgttagata atattcaacg
     1201 tagcaaacga aacggtttaa ttattgctat ctctatcggt tgtgggctgg ccgtaacaac
     1261 tcgacctgag ctattggata agcttccaca cttctttaaa gaagtttttg gctcaggtat
     1321 tacggtcggc tccttattag cacttatttt gaatttagtt ttacctgaag ataaaataac
     1381 caagaaagaa aataagagtg atgaataaat caccctaaaa aaacaaggac agctcacaat
     1441 acaactttga
//
LOCUS       DANLXF010000328         1443 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.329, whole genome shotgun sequence.
ACCESSION   DANLXF010000328 DANLXF010000000
VERSION     DANLXF010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.329"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..405
                     /locus_tag="PXD66_002491"
     CDS             <1..405
                     /locus_tag="PXD66_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320730.1"
                     /translation="EAVTDSRQSIFSSFWVKVWIILLPLALFYCGYNLYIFLNGSESE
                     VSHNTEQAKFASDENHNDIVIHLLPTIGKPLSTKWRITGELKRDNKSFVILADNENRL
                     RLEPSSQFSFSGRMLQGEIDGGIVSYFSGESK"
     gene            402..>1443
                     /locus_tag="PXD66_002492"
     CDS             402..>1443
                     /locus_tag="PXD66_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspD"
                     /protein_id="HDL6320731.1"
                     /translation="MKIHQLGLLLMISSFSYAKNVDFKLEAVPLPKAISIIYDEVLQK
                     PYMLDPKLSNDSRVISFHTTEQQNFEQFIQRYFSNMNISVSEKKGVVYIGHIEPKQPK
                     VIKRSFVYSPIHRDVDYLASFLKDGTEGNQVFASGDKLVYYGTSEDIARVKSILKSID
                     TKSREVVVTGYVFEVQNIEKEGSGINLLAKLLSGKLGISVGYKQSYENFITVNAGSLD
                     AMFELFRTDQRFRVVSSPTLRVKSGSRGNFSVGSDVPVLSNVTYQDGRPIQSIEYRSS
                     GVIFDIQPTIKDKAIDLKIQQLSNFIKTDTGVNNSPTLVKRDLVTDVTVKSGDVIVLG
                     GLAENKISNGETS"
BASE COUNT          485 a          207 c          303 g          448 t
ORIGIN      
        1 gaagcggtta cagatagtag gcaatctata ttttcatctt tttgggtcaa ggtatggatt
       61 attcttttac cattggcatt attttattgt ggctataacc tttacatttt cctaaatggt
      121 tcagagagtg aagtaagcca taatactgaa caggctaaat ttgcttctga tgaaaaccat
      181 aacgatatag taattcattt acttcctact attggaaaac ctttatcaac taaatggcga
      241 ataacagggg aactaaaaag ggataataaa tcatttgtga tcttagctga caatgaaaac
      301 cgtttaagac ttgagcctag tagccagttt agttttagcg gtcgaatgtt gcaaggagag
      361 atagatggtg gaatagttag ttatttttcg ggagaatcta aatgaaaatc catcaattag
      421 ggctattgct catgattagc tcattttctt atgctaagaa tgttgatttt aaattagaag
      481 ctgtgccgtt accgaaagca atatcaataa tatacgatga agtgctacaa aagccatata
      541 tgcttgatcc taaattatct aatgattcaa gggttattag ctttcataca acagagcaac
      601 agaattttga gcagtttatt caacggtatt tcagcaatat gaatatcagt gtatcagaga
      661 aaaaaggtgt tgtttatatt ggacatattg aaccaaaaca gccgaaagta ataaaacgta
      721 gttttgtgta ttcgcctatt catcgtgatg ttgattattt ggctagcttc ctgaaagatg
      781 gaacagaagg aaaccaagtt tttgcaagtg gcgacaagct tgtatattat ggtacttctg
      841 aagatattgc acgagtgaaa agtatcttga aatcaatcga cacaaaaagc cgtgaagtag
      901 tggtaactgg ttatgttttt gaagtgcaga atattgaaaa agagggtagt ggcataaact
      961 tgttagcgaa attgctatca ggaaaattag gcattagtgt tggttacaag caaagttatg
     1021 aaaactttat caccgtgaat gcaggaagtt tagatgcaat gtttgaattg tttcgcacag
     1081 atcagcgttt tagggttgtc agcagtccga cattaagagt aaaatcaggc tcaagaggta
     1141 atttttcagt aggctctgat gtccctgtat tatcgaatgt aacctatcag gacggaaggc
     1201 ctattcagtc gattgaatac cgttcaagcg gtgtaatatt tgatattcag ccaacgataa
     1261 aagataaagc gattgattta aaaattcaac aactatcaaa tttcatcaaa acagataccg
     1321 gtgtaaataa tagcccaacg ttggtaaagc gtgatctggt aacagatgtt acggttaaaa
     1381 gcggtgatgt tatcgtttta ggtgggttag ctgaaaataa aatatctaat ggcgaaacca
     1441 gtt
//
LOCUS       DANLXF010000329         1406 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.330, whole genome shotgun sequence.
ACCESSION   DANLXF010000329 DANLXF010000000
VERSION     DANLXF010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.330"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            564..812
                     /locus_tag="PXD66_002493"
     CDS             564..812
                     /locus_tag="PXD66_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320732.1"
                     /translation="MISGEKMTWESLGASAIGGAIGGGIGATMKAGAVTASTLSALGG
                     GMGEGLYKSTSQGIKNSIGIKASCNQSGSDYGDGCDYQ"
     gene            824..1027
                     /locus_tag="PXD66_002494"
     CDS             824..1027
                     /locus_tag="PXD66_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320733.1"
                     /translation="MKIQFPISYQEFRENYFEKKPLLMKGAISQKDLLSWKSINEILP
                     RCDLISEDAIKVMHKGKRAHKKD"
     gene            1066..>1406
                     /locus_tag="PXD66_002495"
     CDS             1066..>1406
                     /locus_tag="PXD66_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_046701250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL6320734.1"
                     /translation="MKITHCKLKKSLQKKLLEFFVAEVTARTAADLLGIQPNTAALFY
                     HKIRLVIEHHLALEAHEIFEGKIEVDESYFGGHRKGKRGRGAAGKVAVFGLLKRQGKV
                     FTKYQIRNANAG"
BASE COUNT          477 a          177 c          314 g          438 t
ORIGIN      
        1 gttatagatg tactgttgct tagtaagtta gatatgagta aggaaaaaaa tgacaaatgc
       61 caaataataa tatatttatt tataataatt ttacttgggg catataagag ctattttatt
      121 caaaaataaa taagaggaga gaaaatgaaa gatcttagta ttaaggattg tgaaataata
      181 tatggaggca tagccccttt gtggtgggat ggtactggca ttacatcata ctgggaacta
      241 aattatacca ccctaccact agtattttag tttatacggc tagataaata tgatttttga
      301 aaaaatatta cttatattga ttatcccatt catattatca agaattgtgt ctattcttct
      361 tgtaaagaag attgtaaaaa ataaaaatgt acaagatgtt attgaggcta tagttctaat
      421 agtatttgtt gtaattataa acatcataga ggaatatctc ttatgaaaga gttaaaaatt
      481 gaggattgcc aatttgtttc ggcaggcttg cacttgttat tgtaggagct gttgctggag
      541 ctgcatatta tgttgggaat ggtatgattt ctggggaaaa aatgacttgg gagagtttgg
      601 gagcatcagc tataggtggg gctattggtg gcggtattgg agcaacaatg aaagctggtg
      661 cggtgacagc gtcaacctta tctgctttag gtggtggtat gggggagggc ttgtataaaa
      721 gtacttctca aggtatcaaa aatagtattg gtataaaggc gagttgcaat caatcaggtt
      781 cggattatgg tgatggatgt gattatcaat aagagagtat attatgaaaa ttcaatttcc
      841 aatttcttat caagaatttc gtgaaaacta ttttgagaaa aaaccacttt taatgaaagg
      901 agcaatatct cagaaagatt tactttcttg gaagtcaata aatgaaatat tacctcgctg
      961 tgatttaatt tctgaggatg caattaaggt aatgcataaa gggaaaaggg ctcataaaaa
     1021 agattagggg ctgtagtaga ttagcatcac tgctatacta caaaaatgaa gataacccat
     1081 tgtaaattaa agaaatcttt gcagaaaaag cttcttgaat tttttgttgc tgaagtgacc
     1141 gctagaaccg ctgctgattt attaggtatt caacccaata cggcagccct gttttaccac
     1201 aaaattaggc tggtcattga acaccattta gcccttgaag ctcatgaaat ttttgaggga
     1261 aaaattgagg tagatgaaag ctattttggt ggtcatcgca aaggaaaacg agggcgaggt
     1321 gcggctggga aagtcgctgt attcggtttg ttgaagcgtc aaggaaaggt gtttacaaaa
     1381 taccaaatca gaaacgctaa tgccgg
//
LOCUS       DANLXF010000330         1348 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.331, whole genome shotgun sequence.
ACCESSION   DANLXF010000330 DANLXF010000000
VERSION     DANLXF010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.331"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            322..576
                     /locus_tag="PXD66_002496"
     CDS             322..576
                     /locus_tag="PXD66_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320735.1"
                     /translation="MICHADPEYVKYNYAGLWEKGSDGTFFCNYQQFMPEVEAKESLR
                     KQEPELDDRYNEEEYSEEEYPDENDASDENDNYDETYNVF"
     gene            776..952
                     /locus_tag="PXD66_002497"
     CDS             776..952
                     /locus_tag="PXD66_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320736.1"
                     /translation="MATSSFDKRFVVKDPKAVKVLIEALQSPIKVVLPKKDLEKEEKE
                     ALCAIKKRLASMGL"
     gene            913..>1348
                     /locus_tag="PXD66_002498"
     CDS             913..>1348
                     /locus_tag="PXD66_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320737.1"
                     /translation="MCNQKTLSLYGLVKELGIEKTQLFLEQAFSCSRNKDVESFLLNK
                     AIRFETALAAATHLIINEQGDILAYFTLSFKEIEVEVSKSQLKKLTAGLSNDSHLRVL
                     LIGQIGKNEKVIDNPAKLPDILNHIYAQLYKAQRVLGGRVVIL"
BASE COUNT          494 a          237 c          232 g          385 t
ORIGIN      
        1 ccgctactca acatacaggg atggttatct tgataacgct cacgatactc ttgagcaata
       61 gctagtaatt gctgattagt catataaagt ctccaataac ataataaacc tgaaaacgtt
      121 cttagtattc tggcaacatt tttataaata gaaaccaaaa actatattca aatcattttt
      181 agtttctgct caacattcct atgtttttcc atacactgaa gtaaatttac ctaaccctat
      241 tttgctgtga aaccaaaact cctcacgctt atccttagta gcatttcgat gtcgctcact
      301 tatgccaccc ctcaattagg gatgatatgc cacgctgatc ctgaatatgt aaaatacaac
      361 tacgccggac tttgggaaaa aggcagcgat ggcacttttt tctgcaatta ccagcaattt
      421 atgcctgaag ttgaagctaa agaatcacta aggaaacaag aaccagaact tgatgataga
      481 tataacgaag aggaatatag cgaagaagaa tatcctgatg aaaatgatgc cagcgatgaa
      541 aacgataact acgatgaaac ttataatgtt ttctagtatg tatatggaaa ccggaggaat
      601 ccgtgattga aaaggcttgc atttccaaat atagtctata tattgtggta attgaaagta
      661 tttctatgct aacaagcata aggctatgta atcgaaaacc acccacaagg cctaaataca
      721 gtattattac agccatgagt aaccatggta atttaaatat ttaaggagtt ctataatggc
      781 aacatcatca tttgataaac gctttgtagt caaagatcca aaagctgtca aggtattaat
      841 tgaggcatta caaagcccaa tcaaagtagt tttacctaaa aaagacttag aaaaagaaga
      901 gaaagaagca ctatgtgcaa tcaaaaaacg cttagcctct atgggcttgt aaaagaacta
      961 ggtattgaaa aaacacaact ctttttggag caagcatttt cttgcagtag aaataaagat
     1021 gttgaaagtt ttctacttaa taaagcaatc agatttgaaa ccgcattagc agcagcaaca
     1081 catttaatca ttaatgaaca gggcgatatt ttggcttact ttaccctctc ttttaaagaa
     1141 attgaggtgg aagtaagcaa aagccaatta aaaaagctaa ctgctggttt aagtaatgat
     1201 tcacatttaa gagtattgct tatcggtcaa atcggcaaaa atgaaaaagt aattgacaat
     1261 ccagccaaat tgccagatat tctaaatcat atctatgcac aactttataa agcacaacga
     1321 gttttaggtg ggcgagtcgt tatattag
//
LOCUS       DANLXF010000331         1344 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.332, whole genome shotgun sequence.
ACCESSION   DANLXF010000331 DANLXF010000000
VERSION     DANLXF010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.332"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(130..471)
                     /locus_tag="PXD66_002499"
     CDS             complement(130..471)
                     /locus_tag="PXD66_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HDL6320738.1"
                     /translation="MKAILCQRAKQLRTNMTEAEKVLWQALRAKRFLAIKFKRQQVIG
                     NYIVDFVCFSAKLVIELDGSQHLDQQNYDNLRTHFLNGQGFRVLRFWNNQIHSDLEAV
                     LDTIFYEITRG"
     gene            complement(619..1251)
                     /locus_tag="PXD66_002500"
     CDS             complement(619..1251)
                     /locus_tag="PXD66_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2625 family protein"
                     /protein_id="HDL6320739.1"
                     /translation="MKNLEQLLDKQSAWLILRDWFTQAKNHYEILASYPEDAGSALVG
                     MQLSTRSPLGAMVYETGGVFIDHGWLRILGSGNEKLPRGLFDWNFGKTFKQSGEQPSH
                     LLVADDAIGGYFAINAGGIGSDLGKVYYYHPKAKKWENTQLGYSEFLGWALAGDLNHF
                     YQDLRWENWQTDVEKLQGHQVLDLTSKIAVPIERHYQGEFAPENMGYSVA"
BASE COUNT          414 a          272 c          217 g          441 t
ORIGIN      
        1 ttctcctttt gtaggagaaa atgttgttct ttcaaaattc taaaataacc gcaaaattaa
       61 ctctcgcccc ttgtgggaga gagacagata aatcattcgt tcagaatgat ttatcagaga
      121 gagggggaat taccctctcg taatttcata aaaaattgta tctaaaacag cctctagatc
      181 gctatgaatt tgattattcc aaaatcttaa aaccctgaac ccctgaccgt ttaaaaaatg
      241 agttcgtaga ttatcatagt tttgttgatc taaatgttgt gaaccatcta attcaataac
      301 taatttcgct gaaaagcaaa caaaatcgac aatatagtta cctataactt gttgccgttt
      361 aaattttatc gctaaaaatc gttttgctct taatgcttgc caaagaacct tctctgcttc
      421 tgtcatatta gtccgaagct gtttagctct ttggcataaa attgctttca ttttttattc
      481 ctctttttta atttagtata aaaggaataa aaaatataat ttgagcgatt tttgtgatct
      541 aaatcgaaaa tcagaaaatt tctttcgttc taaaaaaata agctaaacag cctatatttc
      601 agcctctctt acaaacactt aagccaccga atatcccata ttttcagggg caaattcgcc
      661 ttggtagtgg cgttcaatcg gaacggcgat tttacttgta agatccagca cttgatgccc
      721 ttgtaatttc tctacatcgg tttgccagtt ttcccaacgc aaatcctgat aaaaatggtt
      781 taaatctccg gctaatgccc agcctagaaa ttctgaataa cctaattggg tgttttccca
      841 ttttttagct tttggatgat aataataaac tttgccaaga tctgagccga ttccgccggc
      901 attaatggca aaatagccac cgattgcatc atccgctacc agcaagtggc ttggttgttc
      961 gccagattgt ttaaaagttt tgccaaagtt ccaatcaaat aagcctcgag gcaatttttc
     1021 gttgcctgag cctaaaatgc gtaaccaacc gtggtcaata aatacgccac cggtttcata
     1081 caccattgcc cctagcggtg agcgagtaga aagttgcata ccaaccaatg cactgccggc
     1141 atcttcaggg taactcgcta aaatttcgta atgatttttg gcttgggtaa accagtctct
     1201 taaaattaac caagccgatt gtttatctaa taattgttct aaatttttca tttgcaaaat
     1261 ttccttagaa tttgaccgct tgcgaaaacc atacaaatgt aggattaata gtcaaagaat
     1321 ttttcttcta ctttctcaat ctga
//
LOCUS       DANLXF010000332         1310 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.333, whole genome shotgun sequence.
ACCESSION   DANLXF010000332 DANLXF010000000
VERSION     DANLXF010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.333"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..590)
                     /locus_tag="PXD66_002501"
     CDS             complement(<1..590)
                     /locus_tag="PXD66_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDL6320740.1"
                     /translation="MTELTPKQEAFCFAYIETGNASEAYRQAYETEDMKSETVHRKAK
                     ELMDNGKITARIEELKAEHAERHKLTVDDLLKELEEARILAREKENPNAMTQATMGKA
                     KLLGLDKQVTDPCFDGETGKGDMILSVNCSSIKDGKALDETCILKAGTHEFITRDSFI
                     FYRHIRIDSLDEIKANIEAGYFIQKALINDEAYQQIL"
     gene            complement(587..1012)
                     /locus_tag="PXD66_002502"
     CDS             complement(587..1012)
                     /locus_tag="PXD66_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320741.1"
                     /translation="MKHELNLAGITALMNEPIKATLSFLDFPHIRIERLHHDNGRPFF
                     IGVLSLDLEDHINIEMAGATGFLEALAIIAYHQQLTATPLLVNDDLLSEINKLPHPQR
                     TNPFIDRHGNEITREQMSKEYREKIDYAFAEKRMAEGKE"
     gene            complement(1019..1171)
                     /locus_tag="PXD66_002503"
     CDS             complement(1019..1171)
                     /locus_tag="PXD66_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320742.1"
                     /translation="MPELLIVLGNRIAKLEQQHNAMPSETDSITLGILRKQLSQLLKV
                     AERIKE"
BASE COUNT          307 a          274 c          271 g          458 t
ORIGIN      
        1 agtatttgtt gataggcttc atcattaatt agggcttttt gaatgaaata accggcttct
       61 atatttgcct taatctcatc taacgaatcg attcttatat ggcggtagaa aataaagctg
      121 tcccgtgtga taaattcgtg tgttcctgcc tttaaaatac aagtttcatc aagtgctttc
      181 ccgtctttaa tgcttgagca gtttacactt aaaatcatat ctcctttacc ggtttcacca
      241 tcaaagcacg gatcggtaac ctgcttatcc agccctagca acttcgcttt gcccattgtg
      301 gcttgagtca ttgcgttagg gttttctttc tctctcgcca gtatacgagc ctcttccagc
      361 tcctttaaaa ggtcatcaac ggttaattta tggcgttctg cgtgttcggc tttcagttcc
      421 tcaatccttg ccgtaatctt gccgttatcc attagctctt tagcttttcg gtggacggtt
      481 tcggacttca tatcttcggt ttcataggct tggcggtagg cttcgctagc attgccggtt
      541 tcaatataag caaagcaaaa agcctcttgt tttggtgtga gttcggtcat tctttcccct
      601 ccgccattcg cttttcagca aaggcataat cgattttttc tcggtattct ttgctcatct
      661 gctcccgtgt aatttcgttg ccgtgtcggt caataaatgg gtttgttcgt tgtgggtggg
      721 ggagcttgtt gatttcactc aataagtcat cattcactaa tagcggcgtg gcggttagct
      781 gttgatgata ggcaataatc gctagagcct ctaaaaagcc cgtagcccct gccatttcaa
      841 tattgatatg atcttcaagg tcaagagaaa gcacaccgat aaaaaacggt ctgccgttat
      901 cgtggtgcag tcgttcaatt cggatatgcg gaaaatctaa aaagctcaag gttgctttga
      961 taggttcatt cattaaagca gtaatacccg ctaaattaag ctcgtgtttc attttggttt
     1021 attcctttat tctttccgcc acttttaaca gttggctaag ttgttttctt aaaatgccga
     1081 gtgtgattga atcggtttcg cttggcattg cgttatgttg ttgttccagc ttcgctattc
     1141 ggttgcctag cacaattaaa agctcgggca atgggtaagc tcggtttcag aatagaggct
     1201 taaaaacgtg aatagtctgc taaataatca ggtgttatct cagaaaaatc gagctttttc
     1261 cctagtgctg cttctaattc ttgtttattt aaaagtaaat tttggaaatc
//
LOCUS       DANLXF010000333         1302 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.334, whole genome shotgun sequence.
ACCESSION   DANLXF010000333 DANLXF010000000
VERSION     DANLXF010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.334"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            32..751
                     /locus_tag="PXD66_002504"
                     /pseudo
     CDS             32..751
                     /locus_tag="PXD66_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616566.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            811..1260
                     /locus_tag="PXD66_002505"
     CDS             811..1260
                     /locus_tag="PXD66_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudoazurin"
                     /protein_id="HDL6320743.1"
                     /translation="MLAKLKWNQLACVTLAFCSASVFSAHHEIKMLDNNAEGSMVFEP
                     GYVKAEVGDTVTFIPSHKGHWVESRSIPEGAEKFLSKEDEEFTITLTHEGVYVYYCPP
                     HRTMNMAGIIQVGQALNLEATQKEVEKIEKRTKENKGRLFEYLEQVK"
BASE COUNT          425 a          229 c          291 g          357 t
ORIGIN      
        1 gttttagtcg ttatctagga cagcccctac ttttaatact cgtaagcccc cttttgataa
       61 ccctaaagtt cgtcaagcat tgaattatgc ggtgaataaa actgaaattt taacttccgt
      121 ttaccaaggc tctgcaacgc cagctaaaaa cccaatgcct tctacgattt ggggatataa
      181 caacgaagta gccggttatg aatttaatcc ggaaaaggcg aagagcttgc ttaaagaggc
      241 cggattagaa aatggctttg aaacagaaat ttgggcaatg cctgtttctc gcccttacaa
      301 tccaaatgcc cgtagaatgg ctgaaatgat tcaagaggat tggaaagcaa taggggtaga
      361 ggcaaagata gtgagctatg aatggggaga atatcttaaa cgaatgggaa acggcgagca
      421 cgctaccgga atgattggtt ggaccggaga taacggcgat ccagataatt tcctcaatgc
      481 cctactcagt tgtaatgcgg tagaacaagg ctcaaactat gcagggtttt gtaatccaga
      541 gtttgaccgt ttaattaatg aggctgcgac tatcagtgat caaaacaagc ggtcagattt
      601 atataaaaaa gcgcaattca tctttaatga gcaagcacct tggttaccga ttgcacattc
      661 aacggtgtat tttccggtaa ggaaagaggt aaaaggctat gttgtcagcc cgtttattgc
      721 tcataatttt tatggtgttg agctggaata acctaaggta taatgaattg aactcatttt
      781 gtgcctcata aatttaggag caataaatat atgttagcga aacttaagtg gaatcaatta
      841 gcttgtgtaa cattggcatt ttgttcagcc tcagtatttt cagcccatca cgaaatcaaa
      901 atgttagata acaatgcgga aggatcaatg gtatttgagc ccggttatgt aaaagcagag
      961 gtgggcgata cggtaacatt tattccgtct cataaagggc attgggttga aagccgctcg
     1021 attccggaag gtgcggagaa gttcttatct aaagaagatg aggaatttac tattaccctt
     1081 actcacgaag gtgtttatgt ttactactgt ccgccacacc gaaccatgaa tatggccgga
     1141 attatccaag taggacaagc tctcaatctt gaagccacac aaaaagaagt ggaaaaaatt
     1201 gaaaaacgca cgaaagaaaa taaaggacgt ttatttgaat atttagagca agttaagtag
     1261 aagtgattta attttgcgat ctgcatcgaa aaatcaatat tt
//
LOCUS       DANLXF010000334         1300 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.335, whole genome shotgun sequence.
ACCESSION   DANLXF010000334 DANLXF010000000
VERSION     DANLXF010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.335"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..350
                     /locus_tag="PXD66_002506"
     CDS             <1..350
                     /locus_tag="PXD66_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-1-phosphatase/inositol phosphatase"
                     /protein_id="HDL6320744.1"
                     /translation="ENKITLLVGHDSNIIALLSALNVKPYKLAHQYEQTPIGGKVFIE
                     IWKEKSSQAQKVKLEYVYQSTDQIRKAIPLSLDKPPQHHILSLDYCQADQYGLCDYME
                     FKQLLEKVIADTL"
     gene            658..>1300
                     /locus_tag="PXD66_002507"
     CDS             658..>1300
                     /locus_tag="PXD66_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL6320745.1"
                     /translation="MSQDIQADLKKILALLSRNRRFQSLFLLLLRSEQFINSDALSTE
                     LQLSVRTVKKEIKLLKEKLEPYGIQIFSRPFYGYKLLIQFEELHTQLKQYFQISQPMT
                     VNNKFESRVNAILRRLLVSQRALKVEDFQSELYLSFTSALTKEFSQVKALLGKYGLIL
                     EAKPYHGMNIVGESYRKIMLTVRLYKYFTQLEHPFGIKEFEQCFACPMTEKIRE"
BASE COUNT          460 a          215 c          226 g          399 t
ORIGIN      
        1 gtgaaaataa aataacactt ttagtcggac acgattccaa tattatagcc ttactttctg
       61 cattaaatgt taagccttat aaattagcac atcaatatga acaaactcca ataggaggga
      121 aagtctttat tgaaatctgg aaagaaaaaa gtagccaagc acaaaaagtt aaactggaat
      181 atgtatatca atctactgac caaattcgta aagcgattcc actaagcctt gataaaccac
      241 cgcaacatca cattttgagt ttggattatt gccaagctga ccaatatgga ttatgcgatt
      301 atatggaatt taaacagtta ctggaaaaag tcattgctga tacattgtga gattttgaca
      361 aagtgagtga aaaaggtagc ataaaaaagt cgggacagtt gccagctgct ccctgtaaga
      421 tgttgtttac agcactagta atgctactat ccccaaaaag ctgctgaaag gcagcttttt
      481 tgttgtatat aaaagtacac ctttctacgc ttcattcttt ttctccaatt aaacctaatt
      541 taggacttct agtgataaat tcggtatcat atcttaattc actacatatc gtgcaagcct
      601 taaaattgaa agggctgacg aaatcctatt taattctgac acacataagg gaatattatg
      661 tcgcaagata ttcaagccga tttaaaaaag atattggcac tattaagccg taaccgtcga
      721 tttcaatcat tatttttatt actgttaaga agtgaacaat ttattaatag tgatgcactt
      781 tctacggagt tacagctttc agtacgaacg gtaaaaaaag agattaagtt attaaaagaa
      841 aaacttgaac cttacggcat tcagattttt tctcgaccgt tttatggcta taaattattg
      901 attcagtttg aagaattaca cacgcaacta aaacagtatt tccaaatatc acagccgatg
      961 acggtaaata ataaatttga gtctcgggta aatgcgattt tacgccgatt actggtgagc
     1021 cagcgtgcat tgaaagttga agattttcag agtgagcttt atttaagttt taccagtgcc
     1081 ttaacaaaag aatttagtca agttaaagcc ttgttaggaa aatatggatt gatactcgaa
     1141 gcgaaaccct atcacggaat gaacattgta ggtgaaagtt atcgtaaaat aatgctcaca
     1201 gtacgattat ataaatattt tacccagctt gaacatccat ttggaattaa agaatttgaa
     1261 cagtgttttg cttgtcctat gactgaaaaa atccgtgaaa
//
LOCUS       DANLXF010000335         1293 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.336, whole genome shotgun sequence.
ACCESSION   DANLXF010000335 DANLXF010000000
VERSION     DANLXF010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.336"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..707)
                     /locus_tag="PXD66_002508"
     CDS             complement(<1..707)
                     /locus_tag="PXD66_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005712160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL6320746.1"
                     /translation="MSIYRRKANGPWWVDITTPNGKRIKRSTRTLVKREAQQYHDKLK
                     QEMWAEDKLEEKRKYIFEDALLHYVRAAEELKDKATKKRHAEYWLSKFAGRELSSLTA
                     QEIILNIPKKNANTKKPLSHSTQNKYVKSLSRVLNIAHKLGMLDAVPHIPKKKEPPIR
                     VRWITKEQAKQLIDKLSSDWMKSICKFALMTGARRTEILTMTWDKIDLVRKVAIVTND
                     VAKSGKARSLLLNQEAI"
     gene            complement(611..934)
                     /locus_tag="PXD66_002509"
     CDS             complement(611..934)
                     /locus_tag="PXD66_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL6320747.1"
                     /translation="MEHLTINQVAKELNCHPNTVRQHLADWGFFKMVGSEVWRINRED
                     LERAKKRAGIDPIRKRSDNDRGLSLSVEENEKCRSTDEKQTVRGGLISQPQTARELRE
                     ALARL"
     gene            complement(1015..1182)
                     /locus_tag="PXD66_002510"
     CDS             complement(1015..1182)
                     /locus_tag="PXD66_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDL6320748.1"
                     /translation="MQFTYGSICSGIEATSIAWHGLGKPLWFSEIEPFPCAVLAHRFP
                     NVPNGNGMAVP"
BASE COUNT          316 a          295 c          231 g          451 t
ORIGIN      
        1 ttaattgcct cttgattcag taatagcgat cgtgccttac ctgatttagc gacatcatta
       61 gtcacgattg ctacctttct tacaagatct atcttatccc aagtcattgt caaaatttct
      121 gtccgtctgg ctccagtcat caaagcaaac ttacagatag atttcatcca gtctgaactt
      181 aatttatcta ttagctgttt tgcttgttct ttggtgatcc agcgtacacg aattggcggc
      241 tctttctttt tcggtatgtg tgggactgca tccaacatac caagtttatg agcgatattt
      301 aatactctcg ataaagattt cacatactta ttttgcgtac tatgagatag aggctttttc
      361 gtatttgcat tctttttcgg gatatttagg ataatctcct gagctgttaa agagctgagt
      421 tcacgaccgg caaatttgga taaccagtat tcagcatgtc gcttttttgt ggctttatct
      481 tttaattctt cggccgcacg aacgtaatgg agcaaagcat cttcaaagat atattttctc
      541 ttttcctcga gtttatcctc tgcccacatt tcttgcttga gtttatcgtg atactgctga
      601 gcttcacgtt ttacaagcgt gcgagtgctt ctcttaattc tcttgccgtt tggggttgtg
      661 atatcaaccc accacggacc gtttgctttt cgtctgtaga tcgacatttt tcattctcct
      721 cgaccgacag agataaccct cggtcattat cagatcgttt tcttattggg tcaataccgg
      781 ctctcttttt tgctctttcc aaatcttctc ggttgatccg ccaaacttcc gagcctacca
      841 ttttaaaaaa tccccaatcc gccaaatgtt ggcggactgt atttggatgg caatttaatt
      901 cttttgccac ttgattaata gtaagatgtt ccattcatta ctttctccaa aataaatccc
      961 tctaaacgag ggattgtact aaataatcgt taagtctttg ccttatccac tgcatcaagg
     1021 cacagccata ccgtttccat ttggcacatt ggggaaacgg tgagctaata ccgcacaggg
     1081 gaatggctca atttcagaga accacaaagg cttacctaaa ccgtgccacg ctatacttgt
     1141 agcttcgata ccgctacaaa ttgagccgta ggtgaattgc attttttacc tatcctatcg
     1201 aaatactcaa attcccaata cggacaacat tttcttcgtt ggcttctagg atttctctca
     1261 acttagcata ggttttgggg catcttattt gac
//
LOCUS       DANLXF010000336         1283 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.337, whole genome shotgun sequence.
ACCESSION   DANLXF010000336 DANLXF010000000
VERSION     DANLXF010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.337"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            226..942
                     /locus_tag="PXD66_002511"
     CDS             226..942
                     /locus_tag="PXD66_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HDL6320749.1"
                     /translation="MYLMYVDESGSTTDPTQKYFVLSGIAVFERNTHWIEKKMNVIAS
                     QFCQYSEGSDPYFYELHGSPMRSGKSEWRGIPMSERINAIQDCLKLVQSNNIRIFAAV
                     IEQGYSSGQDTITECFEQISSRFDMYLSRLHAQGNTQRGISIFDKSSTEKSIQALART
                     FKNSGHTYGKLRNFSEVPLFLDSKSSRLIQLADLVSYAIFRHYEHNDSSFYDIIRNNF
                     DYHNGRIHGLYLRNKEDIIS"
     gene            complement(985..>1283)
                     /locus_tag="PXD66_002512"
     CDS             complement(985..>1283)
                     /locus_tag="PXD66_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL6320750.1"
                     /translation="KQTDNCQLFNLNLFSRCSRRRGQPMSQNQLTHIFRHISDLVGFT
                     SSPHRFRHTVATQLMKKPENVYVVQKLLGHKDISVTLGYIEHDVEMLRETVNLL"
BASE COUNT          404 a          212 c          233 g          434 t
ORIGIN      
        1 cgatccaggt cttacacatt ctttcaagtt tgtaaagcct gaaaattatt ttttatactt
       61 gatttttgtc aaaaagtctc tacactatat ggcagggtca gttttcgctg tttaacggtg
      121 tttaatgaga ttttcattac tggctctcct taatagaaat ttatggaaga gctatctgat
      181 agagtaatct agcgatagct ctttttcatt gaacaaagag gctatatgta tcttatgtat
      241 gttgatgagt ctggttctac gacagatcct actcaaaaat attttgtatt atcaggtatc
      301 gctgtatttg aacgaaatac tcattggatt gagaaaaaaa tgaatgtgat agcctctcaa
      361 ttctgtcaat attctgaagg gagtgatcca tatttctatg agttacatgg ttcaccaatg
      421 agatctggaa agagtgaatg gagaggcatt ccaatgagtg agcgtattaa tgccattcaa
      481 gattgcttga aattagttca aagtaataat attcgtatat ttgcagcagt tatagagcaa
      541 gggtattcta gtggtcagga tactattaca gaatgttttg agcaaatatc atctcgattt
      601 gatatgtatc tgagccgttt acatgcccaa ggtaataccc aaagaggcat ttctatcttt
      661 gataaatcat cgactgagaa gtcaatccaa gcacttgcaa gaacattcaa aaatagtggg
      721 catacttatg ggaaacttag aaatttctca gaagttccat tatttcttga ctcaaagtca
      781 tcgagattaa ttcagctagc ggatctagtt tcctatgcga tatttagaca ctatgaacac
      841 aatgatagct cattttacga tattataaga aataactttg attatcataa cgggcgtatt
      901 catggtttat atttacggaa caaagaagat attatttcat aaaaattcgc cccaaaaggg
      961 agaagtatct cccttttgta ttttttataa caaattaacc gtttctctca acatttccac
     1021 atcgtgttca atatatccta gtgtcacact gatatcttta tgccctaaca gtttctgaac
     1081 cacataaaca ttttcgggct tcttcattaa ttgagtagca actgtatgtc gaaatctgtg
     1141 tggtgatgaa gtaaaaccga ccaagtcaga aatatgccga aatatatgtg taagctgatt
     1201 ttggctcatc ggctgtcctc ttcttcgaga gcatcgagaa aataaattga gattgaacaa
     1261 ttggcaatta tctgtttgtt ttc
//
LOCUS       DANLXF010000337         1280 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.338, whole genome shotgun sequence.
ACCESSION   DANLXF010000337 DANLXF010000000
VERSION     DANLXF010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.338"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..>1280
                     /locus_tag="PXD66_002513"
     CDS             <1..>1280
                     /locus_tag="PXD66_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320751.1"
                     /translation="DKGTKLASSQVYNQADGKLYTWNGTAYSATVAAEDVTGKLSKSK
                     IDTSLISQLTGADNTANLARRLAETAQSNINQEITNRQDAVTAEANNRTKAIQAESAN
                     LTKKIQAEATARGAAVTQLQNVDAQQAQLISAVTAKANNALSGLEEEKTARANGDKAE
                     SQAREALTARMGAAESNIVTIQRTVANNAQSISEVSQNLNAKIDNINIGGRNLLRDSE
                     FNAYNKWGSPQIEFAENANRRTIKVTSTGTNGPVGIVSSNRHSTSYFQQGETYTLSLF
                     ARGSKALDYLYLMRQDGNNVRLPVINVASETEFNYYKLTFKAPFTTQQGYFLVGFFQS
                     TPNQFVEFHSLKLEKGNVATDWTPAPEDVDSAVSAVSADLTSYKQTQAATDSAQAQQL
                     NQLSVNLTKAESNLNAKITEEKNARVNADKANAE"
BASE COUNT          452 a          263 c          261 g          304 t
ORIGIN      
        1 gacaaaggta ctaaactggc gtcatctcaa gtttacaatc aggctgacgg caagctctat
       61 acttggaacg gtacagcata ctcagctaca gtggctgcgg aagatgttac cggcaaatta
      121 tcaaaatcta aaattgatac gtcccttatt tcgcaattaa caggtgcaga taacaccgca
      181 aatttggcaa gaagattagc ggaaacagct caaagcaaca ttaaccaaga gattactaac
      241 cgacaagatg ccgttactgc cgaagcaaat aaccgtacta aagccattca agccgaaagt
      301 gcgaatttaa cgaaaaaaat tcaagctgaa gcaaccgctc gtggggctgc tgttacacag
      361 ttacaaaatg ttgatgctca acaagcacaa ctcatttctg ctgtcacagc aaaagctaat
      421 aatgctctat caggcttgga agaggaaaaa acagcacgag caaacggcga taaagcggag
      481 tctcaagcac gtgaagcatt aacagcaaga atgggagcag ctgaaagcaa tatcgtaact
      541 attcaacgta cggtagcaaa taatgcccaa agcattagtg aagtcagtca gaatttaaat
      601 gctaagattg ataatattaa tatcggcggt cggaatttat taagagacag cgagttcaat
      661 gcgtacaata aatggggtag tccacaaatt gaatttgctg aaaatgcaaa tcgccgaact
      721 atcaaagtaa cctcaacagg tactaacggt cctgttggta ttgtttcatc taatcgccat
      781 tccacatctt atttccaaca aggagaaact tatacactct cattatttgc tagaggaagt
      841 aaggcactag actatctcta tctaatgcgt caagatggga ataacgttag attaccagtg
      901 attaacgttg cctctgaaac agagttcaat tattacaaac tgacgttcaa agcaccgttt
      961 accacacaac aaggctattt cttagttggt tttttccaat ctactccaaa tcaatttgtt
     1021 gagtttcata gtttaaagct agaaaaaggc aacgtagcca cagactggac gccagcaccg
     1081 gaagatgtgg acagtgcggt tagtgcagtt tccgctgatt taacgagtta taaacaaact
     1141 caggcggcta ccgattcagc acaagcacag caacttaatc agttatctgt gaacttgacc
     1201 aaagcagaga gcaatttaaa tgccaaaatc acggaagaaa aaaatgctcg tgtgaatgcc
     1261 gataaagcga atgcagagaa
//
LOCUS       DANLXF010000338         1243 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.339, whole genome shotgun sequence.
ACCESSION   DANLXF010000338 DANLXF010000000
VERSION     DANLXF010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.339"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            7..1221
                     /locus_tag="PXD66_002514"
     CDS             7..1221
                     /locus_tag="PXD66_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDL6320752.1"
                     /translation="MNPSPRLLMCPGPTDADPRVLRAMSAPILGQFDPEFTSLMNETM
                     EMERRLFQTKNEQTYVVNSTSRGGLETVLTAAICPGDKVLIPAFGRFGYLLNEILARS
                     GADITIIEREWGTVFEPEEIEAELKKGGYKAVAIIHGETSTSMMQPLEEIGKICKQYD
                     AMLIVDTVATLGGVDIRVDEWGIDACIGGTQKCISAPSGTALITYNKRIEEIIAKRKR
                     VEKGIRAESDVDGTLPPIPSNYLDLAQLQDYWSPRRLNHHTETTSAQYGVHEALRIIL
                     QEGLEARFARHILNDKALCAGLEAMGLKIFGDRKHKAPVVTAFHIPEGMSGPALRSDI
                     LNHFGIELATSFGPLDGKVIRIGNMGFSSQKRNVLLTLGALEAVLLSHKVKVPSGDAV
                     AAALAVYKEADK"
BASE COUNT          381 a          257 c          265 g          340 t
ORIGIN      
        1 agagaaatta atccctcccc tcgtttatta atgtgccctg gcccaacaga tgctgaccca
       61 cgtgttttgc gtgcaatgtc tgcacccatt ttagggcaat ttgaccctga gtttacctca
      121 ttaatgaatg aaacaatgga aatggaacgc cgtttattcc aaaccaaaaa tgagcaaact
      181 tatgtagtca actccacctc tcgtggtggc ttagaaactg ttctaaccgc agcgatttgc
      241 ccaggcgata aagtgttaat tcctgcattt ggtcgttttg gttatttatt aaatgaaatt
      301 ttagctcgta gcggtgcaga tattaccatt atcgaaagag agtggggaac cgtatttgaa
      361 ccagaagaaa ttgaagcaga gttgaaaaaa ggcggctata aagcagttgc tattattcac
      421 ggcgaaacct caacctctat gatgcaaccg ttagaagaga tcggcaaaat ctgtaaacaa
      481 tatgatgcaa tgttaattgt agataccgtt gcaaccttag gcggtgtaga tattcgtgtg
      541 gatgaatggg gcattgatgc ttgtatcggc ggtacacaaa aatgtatttc cgccccatca
      601 ggcacagctt taatcactta taacaaacgg attgaagaga ttattgcaaa acgtaaacgt
      661 gtggaaaaag gcattcgtgc agagtctgat gtagatggca cattaccgcc aattccaagt
      721 aactacttag acttggcaca gctacaagat tactggagcc cacgccgttt aaatcaccac
      781 acagaaacca cttctgcaca atatggtgta cacgaagcgt tacgcattat tttacaagaa
      841 ggcttagaag ctcgttttgc tcgccatatt ttaaacgaca aagcattatg tgcaggttta
      901 gaagcaatgg gtttgaaaat ttttggcgat cgcaaacata aagcccccgt tgtaacggca
      961 ttccacattc cagaaggtat gagtggccca gcattgcgta gtgatatttt aaaccacttc
     1021 ggtattgaat tagccacctc attcggcccc ttagatggaa aagtaatccg tatcggtaat
     1081 atgggtttct caagccaaaa acgtaacgta cttttaacct taggtgcgtt agaagccgta
     1141 cttttaagcc ataaagtaaa agtgccttca ggcgatgcgg ttgcagctgc cttggcagtc
     1201 tataaagagg ctgataaata accaccttcc taaatttaca ata
//
LOCUS       DANLXF010000339         1215 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.340, whole genome shotgun sequence.
ACCESSION   DANLXF010000339 DANLXF010000000
VERSION     DANLXF010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.340"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..170
                     /gene="csrA"
                     /locus_tag="PXD66_002515"
     CDS             <1..170
                     /gene="csrA"
                     /locus_tag="PXD66_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HDL6320753.1"
                     /translation="KIGEVLLVGDDVEITVLSIRGNQVKLGVNAPKEISVHRREIYER
                     IKASVEEENAE"
     gene            306..1193
                     /gene="galU"
                     /locus_tag="PXD66_002516"
     CDS             306..1193
                     /gene="galU"
                     /locus_tag="PXD66_002516"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDL6320754.1"
                     /translation="MKVIIPVAGLGTRMLPATKAIPKEMLILADKPLIQYIVNECVAA
                     GFKEIVLVTHSSKNAIENHFDTSFELETMLEKRVKRQLLDDVRSIVPKDVTLIHVRQG
                     QAKGLGHAVLCGRTVVGDEPFAVVLPDVLLGEFTANQKTENLAAMVKRFQETGYSQIM
                     VAPVPMENVSSYGVADCHGVDIPLGGAAPIAKMVEKPSIEEAPSNLAVVGRYVFSSNI
                     WNLLEKTPVGVGDEIQLTDAIDMLIHQETVEAFHMTGRSFDCGYKVGYMKAFVEYSLN
                     HDKCGKEFKAFIKELAKSL"
BASE COUNT          395 a          193 c          277 g          350 t
ORIGIN      
        1 gtaagattgg ggaagtttta ctcgtaggag atgatgttga gattacggta ctcagcatcc
       61 gaggaaatca agttaaatta ggtgtgaatg caccaaaaga aatctctgta caccgccgag
      121 agatttatga aagaataaaa gcctcagtag aagaggaaaa tgcggaataa gcattttaac
      181 tgtaaagctg ctagatgata acagcgaagc aggcaatgca aaattttctg aaaattgacc
      241 gcctgttatg tctaagcggt ttatttcagt tatatctaat atgttaagtc atcaaaggat
      301 tccaaatgaa agtcattatt cctgttgctg gattgggaac gcgcatgtta cccgcaacca
      361 aagctattcc aaaagaaatg cttatccttg cagataaacc tcttattcaa tacattgtaa
      421 atgaatgtgt ggctgcaggt tttaaagaaa ttgtattggt tactcactct tcaaagaatg
      481 ctatcgaaaa ccattttgat acctcttttg aattggaaac gatgctggag aaacgagtaa
      541 aacgtcaatt attagatgat gtacgttcta ttgtaccaaa agatgttacc ttaattcacg
      601 ttcgccaagg gcaagccaaa ggtttaggtc atgcggttct atgtggacga acagtggttg
      661 gcgatgaacc gtttgcggtt gtattaccag atgtattact tggtgaattt acagcaaacc
      721 aaaaaacgga aaatttagct gcgatggtga agcgtttcca agaaaccgga tatagccaaa
      781 ttatggttgc gccggttcca atggaaaatg taagtagcta tggggttgct gactgccatg
      841 gtgtagatat ccctctcggg ggggcagcgc ctattgcgaa aatggttgaa aaaccaagta
      901 ttgaagaggc gccttctaat ttagcggtag taggacgtta tgtattttcc tcaaatattt
      961 ggaatttatt agaaaaaacg ccagtaggcg taggtgatga aatccagctg acggatgcaa
     1021 ttgatatgtt gattcaccaa gaaacggttg aagcttttca tatgacaggg cgctcatttg
     1081 attgtggtta taaagtcggc tatatgaaag catttgtaga atatagttta aatcatgaca
     1141 aatgcggaaa agaatttaaa gcatttatta aagagttagc taaatcactc taagccttag
     1201 agtttagact atgat
//
LOCUS       DANLXF010000340         1207 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.341, whole genome shotgun sequence.
ACCESSION   DANLXF010000340 DANLXF010000000
VERSION     DANLXF010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.341"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            33..1151
                     /gene="malK"
                     /locus_tag="PXD66_002517"
     CDS             33..1151
                     /gene="malK"
                     /locus_tag="PXD66_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005552148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HDL6320755.1"
                     /translation="MANVSLRNVGKSYGDVHISKDINLEINEGEFVVFVGPSGCGKST
                     LLRMIAGLEDITTGELYIGGKLMNEVEPSKRGIGMVFQSYALYPHLDVAENMSFGLKL
                     AGVSKAERDQRVNQVAEILQLAHLLDRKPKALSGGQRQRVAIGRTLVSQPEVFLLDEP
                     LSNLDAALRVQMRVEISKLHKKLNRTMIYVTHDQVEAMTLADKIVVLNAGGIAQVGKP
                     LELYHYPANRFVAGFIGSPKMNFLPVKVIAVEPERVKIELPDANHHNFWIPVSGEGVN
                     VGDNLSLGIRPEHLVPSDQTQVTLHGIVQVVELLGNETQIHLEIPEIKQPTLIYRQND
                     VVLVNEGDDLNIGILPERCHLFKEDGTACKRLFVEKGV"
BASE COUNT          374 a          220 c          270 g          343 t
ORIGIN      
        1 ccaacaagca aaagcagaaa agaggtttag ttatggcgaa tgtatcatta cgtaacgtgg
       61 gaaaatcgta tggtgatgtt catatctcta aggatattaa tttagaaatt aacgaaggag
      121 aattcgttgt tttcgttgga ccgtcaggtt gtggcaaatc tacgctatta cgaatgatag
      181 caggtttgga agatattacg acaggagaac tctatatcgg tggaaaactg atgaatgaag
      241 tagagccttc aaaacgtggt atcggtatgg tgttccaatc ctatgctctc tatccacatt
      301 tagacgttgc cgaaaatatg tcttttggct taaagctggc aggtgtaagt aaagcagagc
      361 gggatcaacg agttaaccaa gttgccgaaa tcttacagct tgcccattta ttagatcgaa
      421 aacctaaagc actttccggc ggacaacgcc aacgggtggc tattggtaga acactggttt
      481 cccaaccgga agtattttta ctcgatgaac cactttccaa ccttgatgcc gcattgcgtg
      541 tacaaatgcg ggtagaaatt tccaaattac ataaaaaact aaaccgcaca atgatttatg
      601 ttacgcacga tcaagttgaa gcaatgacat tagcggacaa aatcgtggta ttaaatgccg
      661 gcggtattgc tcaagtaggt aaaccgttag aactttatca ttacccggca aatcgctttg
      721 tagcagggtt tatcggctcg cctaaaatga actttttacc ggtaaaagtg attgcggtag
      781 agccggaacg ggtaaaaatt gagttacctg atgcgaatca tcataatttt tggattccgg
      841 tttccggcga aggggtgaat gtgggagata acttatcttt aggtatccgc ccggaacatt
      901 tagttccttc ggatcaaacc caagtgacct tacacggtat tgtgcaagtt gtggaattgc
      961 tcggaaatga aacacaaatc caccttgaaa tccctgaaat caaacaacca accttaattt
     1021 atcgtcaaaa cgatgtggta ttagttaatg aaggcgacga tctgaatatc ggtatattgc
     1081 cggaacgctg ccatttattt aaggaagacg gtacagcttg caaacgtttg tttgttgaaa
     1141 aaggggtata acaatttcag tttattactg attttagagg gtattttcaa tcttctaatc
     1201 aattaat
//
LOCUS       DANLXF010000341         1175 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.342, whole genome shotgun sequence.
ACCESSION   DANLXF010000341 DANLXF010000000
VERSION     DANLXF010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.342"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..297)
                     /locus_tag="PXD66_002518"
     CDS             complement(<1..297)
                     /locus_tag="PXD66_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDL6320756.1"
                     /translation="MSIQQKKRHSRWYERIFFSTAVQSILGVMFSSVLPAIYFWGEKF
                     NSIETRSNTIIATSLAFVMIAISLRVLMKYPGNKSASFVFSTVISWYAVLLIVLT"
     gene            complement(301..1109)
                     /locus_tag="PXD66_002519"
                     /pseudo
     CDS             complement(301..1109)
                     /locus_tag="PXD66_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_162680708.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT          409 a          230 c          161 g          375 t
ORIGIN      
        1 tgttaacacg attaataaaa ccgcatacca agaaataacg gtggaaaaaa caaaacttgc
       61 tgacttatta cctggatatt tcattaacac tcttaagctg atagctatca ttacaaaggc
      121 aagagaagtc gctataatcg tattagaacg agtttcaata gaattaaatt tctctcccca
      181 aaaatagata gcgggtaaga cacttgagaa cataacgccc aaaatacttt gaacagctgt
      241 actaaaaaag atacgttcat accaacgaga gtgtcttttc ttttgctgaa tgctcataat
      301 ttatcctttt cttaatttct tataaagtct tcctaaaaga gatctaggca ataaccgtgg
      361 taaggctcta agtataaaaa acatgcttga tgagattatt gattgtaatc ccaactcttt
      421 tttcaatttt gctaattggt actcactctt aatgtaattc caccctttac gccgagcgta
      481 cattgcagaa cctgaacgaa catttaccag tacatctggt aaattataca cctcatattt
      541 ctgtgtaata actcgtaacc ataaattgta atcttccata taaagatgat gctgatagcc
      601 tccaacatgc tcgatcacag attttttata ggctactgcc atatggttaa aaggatttct
      661 tagtaatgct gaaatacgaa tttcatcatc attaatgggc acttgtttta tacccagaga
      721 attagacatt gtttcgtcaa attcttcaac ttgtcccccc aataaaacca catcaggatt
      781 tttactaata aaatcaattt gcttttcaaa tcgagatggt aagcaaatat catcagtatc
      841 cattctaaac acccactcat tagagcagtg ttttaagcct tcatttaagg ccggcctaaa
      901 cccacatttt gtggtaaact aacaacaaca agcggtaatt tttcagtaaa atgttgcaca
      961 attcgctcta aatctccagg aattatacca tcaaaaacca caacaatttc acttgctggt
     1021 aaagtttggg atactaaaga atctaaacac atctctaaat aagctggttt ttctttataa
     1081 tacaaagata ttaatatcga aaacttcaca actacttttc ccactttttc aacttatgga
     1141 ctatatacgt tatttttata gtatgataaa aaagt
//
LOCUS       DANLXF010000342         1168 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.343, whole genome shotgun sequence.
ACCESSION   DANLXF010000342 DANLXF010000000
VERSION     DANLXF010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.343"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(47..427)
                     /locus_tag="PXD66_002520"
     CDS             complement(47..427)
                     /locus_tag="PXD66_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320757.1"
                     /translation="MNNKRIKPEQFADFITQRTGRNLTCPICNSQEHYLHDGYDTFTE
                     LCGEKIIGVPTIPYRIEPSPDEVMKFAAPEFHSLHFNEREGTKYWLDQKARTAVIVTC
                     VCCNNMLFFDRERILEWIKGSQNE"
     gene            complement(478..1137)
                     /locus_tag="PXD66_002521"
     CDS             complement(478..1137)
                     /locus_tag="PXD66_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HDL6320758.1"
                     /translation="MTTLGSRIKAYREQLGISQYEVAERCTSIDNRDKNNKWGQSRIA
                     NYERNNRTPDLDDIEILSKVLGVSPETLAFDTNVQLARPTKSNSYPLISNIQAGLWTE
                     AFDFKDSEGYDYIDTEIDAGPDAFFLRISGMSMEPKFSEGDLVLIDIRKRPHPGDYVA
                     AVNGTGEATLKRYRELGEWSESGNPHFELIPLNPDFPTLSSMKQDIRIIGVAVEHRSY
                     L"
BASE COUNT          326 a          247 c          211 g          384 t
ORIGIN      
        1 taaggatagt atgattgcat ctctaggatc attttgaatc attggtctat tcattttgtg
       61 agcccttaat ccattctaaa attctctctc tgtcgaaaaa aagcatatta ttacagcaaa
      121 cacaagtgac aataacggct gttctcgctt tttggtctaa ccaatatttt gttccttccc
      181 gttcattgaa atgcaaagaa tgaaattcag gagctgcaaa tttcataact tcatcaggag
      241 atggctctat tcggtatgga atggttggaa caccgataat tttctctccg cataattccg
      301 taaaagtgtc atagccatcg tgtaaataat gttcttggct attacaaata gggcaagtta
      361 aattccgccc agtacgttgc gtgataaaat ccgcaaactg ttcaggttta atgcgtttat
      421 tattcatttt aacttccttt ctacccaata aaattcaact gttagctttg gtggacttta
      481 caaatagctt ctatgctcaa cagcgacacc aataatacga atatcttgtt tcattgagct
      541 gagtgttgga aagtcggggt taagcggtat tagctcaaaa tgaggattgc cggattcaga
      601 ccattcgcct aactcccgat aacgttttaa agtagcctca cctgtgccat taaccgctgc
      661 tacataatcg cccgggtgag gacgtttgcg aatatcgatt aacaccagat cgccttcact
      721 aaatttaggc tccatcgaca ttccggaaat tcttaaaaag aaagcgtccg gtccggcatc
      781 aatttccgta tcgatatagt cgtagccctc cgaatctttg aaatcaaagg cttctgtcca
      841 taaacccgct tgtatattgc tgatcaacgg atatgagttc gattttgtcg gtctggcaag
      901 ctgaacgttt gtatcaaaag caagtgtttc tggagaaaca ccaagcactt tgctgagtat
      961 ttcaatatca tcaagatcgg gagtacgatt gtttctctca taattagcaa tgcgagattg
     1021 cccccatttg ttatttttat ctcgattatc tattgatgta catctttcag caacttcata
     1081 ttggctgatt cctagctgtt ctctgtaagc cttaattcta ctacctaagg ttgtcatagc
     1141 actctcctat tagattttcc tcatttta
//
LOCUS       DANLXF010000343         1163 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.344, whole genome shotgun sequence.
ACCESSION   DANLXF010000343 DANLXF010000000
VERSION     DANLXF010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.344"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            204..479
                     /locus_tag="PXD66_002522"
     CDS             204..479
                     /locus_tag="PXD66_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL6320759.1"
                     /translation="MAKATVARRIKNAINGNFGDHKSLAGTGGLYEMRIATGAGYRIY
                     YAQQGNVIYILLHGGDKSTQQADIEKAKALWAELKTTGESNGNNTNQ"
     gene            457..780
                     /locus_tag="PXD66_002523"
     CDS             457..780
                     /locus_tag="PXD66_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative addiction module antidote protein"
                     /protein_id="HDL6320760.1"
                     /translation="METIQINEQAAEKHGLRPFDVAEFLTDEATIQAYLAEVLKEGDQ
                     DEFLEALNNVARARGMAELAKQTGLGRESLYKTLAKGSKPRFDTIQKILGAFNLVLIP
                     QVKNG"
BASE COUNT          421 a          217 c          228 g          297 t
ORIGIN      
        1 taatttaaaa gcgtgaacaa tgttcaatga tatgaataat tgaaactgct ctcttttcaa
       61 aatttaacca cttgattatt tgtatcttaa tggatacact aagcaataaa tagaataaga
      121 ggctggcaat gacagagcaa accactaatc aaatcatcat taccgataat ttttcaaaat
      181 agcttgagaa tttaaagaac cctatcgcaa aagcaacggt agcaaggcgg ataaaaaatg
      241 cgattaatgg caactttggc gatcataaat ctttagccgg tacaggcgga ctttatgaaa
      301 tgcggatagc aacaggagcc ggatatagaa tttattacgc acaacaagga aatgtaattt
      361 atatcttgtt acacggcggc gataaatcaa cacagcaagc cgatatagag aaagcgaaag
      421 cattatgggc tgaattaaaa actacagggg aaagcaatgg aaacaataca aatcaatgaa
      481 caggcagcag aaaagcacgg tttacgccct tttgacgtgg cggagttttt aaccgatgag
      541 gcaactattc aagcctattt agcggaagtg ttaaaagagg gcgaccaaga cgaattttta
      601 gaagccttga acaatgtggc aagggcaagg ggaatggcgg agcttgccaa gcaaacaggg
      661 ctagggagag agagcctata taaaacccta gccaaaggca gcaaaccacg ctttgacact
      721 attcaaaaaa ttctaggggc attcaattta gtgcttattc cacaagtcaa aaatggctaa
      781 aacttatcac ttgctacctg ccaaaacctc acattcaaat agccgttgat tgatattaac
      841 ggctttttta ttgcttaatt ctgtaccaat tacgcaccga ttgcggatca atacggggca
      901 agttttcgca aaaccttaat gaaatatgac cgcttgtgat tttgtacaaa acgacacaaa
      961 ccaatgtata aaatcaaaac ttgccacaaa tggcggaatg atattaaatg ccccacaaac
     1021 tagcagtatt tcttaatgac ttttcatttt aagggtacat ttaagggtat gtttaacaag
     1081 tcttaaatgc acttctcaat aataacaagc ctcatagcat acaattcaaa ctcggctggg
     1141 gaccatttaa gcgttgcata ttg
//
LOCUS       DANLXF010000344         1157 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.345, whole genome shotgun sequence.
ACCESSION   DANLXF010000344 DANLXF010000000
VERSION     DANLXF010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.345"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            19..1023
                     /gene="gap"
                     /locus_tag="PXD66_002524"
     CDS             19..1023
                     /gene="gap"
                     /locus_tag="PXD66_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010129843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDL6320761.1"
                     /translation="MAIKIGINGFGRIGRIVFRAAQHRDDIEVVGINDLIDVDYMAYM
                     LKYDSTHGRFDGTVEVKDGQLVVNGKAIRVTAERDPANLKWNEIGVDIAVEATGLFLD
                     DETARKHITAGAKKVVLTGPSKDATPMFVNGVNFDTYAGQDIVSNASCTTNCLAPLAK
                     VIHEKFGIKEGLMTTVHATTATQKTVDGPSAKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPALNGKLTGMAFRVPTTNVSVVDLTVNLEKPATYAEICAEIKRASENEMKGVLGYTE
                     DAVVSTDFNGATETSVFDAAAGIALTDTFVKLVSWYDNEVGYSNKVLDLVAHVYNYKG
                     "
BASE COUNT          375 a          217 c          243 g          322 t
ORIGIN      
        1 atataggtag aaaaatctat ggcaattaaa attggtatta acggctttgg ccgtatcggt
       61 cgtatcgttt tccgtgcagc acaacaccgt gatgacatcg aagtggtagg tatcaacgac
      121 ttaatcgatg ttgattatat ggcttatatg ctgaaatatg attcaactca cggtcgtttt
      181 gacggtactg ttgaagttaa agacggtcaa ttagtagtta acggtaaagc gatccgcgta
      241 acagctgagc gtgacccggc taacttaaaa tggaatgaga tcggtgttga tatcgcagtt
      301 gaagcaacag gtttattctt agatgatgaa actgctcgca aacacatcac cgcaggtgcg
      361 aagaaagttg ttttaaccgg tccgtctaaa gatgcaacac ctatgttcgt aaacggcgta
      421 aactttgata cttacgcagg tcaagacatc gtgtctaacg cttcttgtac aactaactgt
      481 ttagcaccat tagcgaaagt tattcacgaa aaattcggta tcaaagaagg tttaatgaca
      541 actgttcacg caactactgc aacgcaaaaa acagtagatg gtccatcagc gaaagactgg
      601 cgtggtggtc gtggcgcgtc acaaaacatc attccttcat caacaggtgc agcgaaagca
      661 gtaggtaagg tattacctgc attaaacggt aaattaaccg gtatggcttt ccgtgttcca
      721 accactaacg tttctgttgt tgatttaact gtaaacttag aaaaaccagc aacttatgca
      781 gaaatctgtg ctgaaatcaa acgtgcttca gaaaacgaaa tgaaaggcgt attaggctac
      841 acagaagatg cagttgtttc aacagacttc aatggtgcaa ctgaaacttc agtatttgat
      901 gcagcagcag gtatcgcatt aactgatact ttcgttaaat tagtatcttg gtacgataac
      961 gaagttggct actcaaacaa agtattagac ttagttgctc acgtatataa ctacaaaggt
     1021 taattgctaa gttatatttt aaatatagag ccaaattctg aacggaattt ggctcttttg
     1081 ctgcgaacaa taaattgaat atagtcgcgt tttcccataa aatcaatttc ctacaaacag
     1141 gaactaagca cagtaag
//
LOCUS       DANLXF010000345         1152 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.346, whole genome shotgun sequence.
ACCESSION   DANLXF010000345 DANLXF010000000
VERSION     DANLXF010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.346"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..>1152
                     /locus_tag="PXD66_002525"
     CDS             <1..>1152
                     /locus_tag="PXD66_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HDL6320762.1"
                     /translation="EKAITFIEAKVGSALQPGWYLVNQAPKSRYDSENRYVHYNKSPI
                     KLIAWGYFNGIINAATNLHTVSPNLDIEKLRQFITDLRLSFPIQAPEVTNDDMHHPNE
                     IRNLVIALNLVQDPTEHIDELPKKVYPSDLFNFGKAQKTLVGSVSIIYRNVWNEIRTQ
                     HFDGEDAILKALKLVSNRIYQGNSSPQSVNVFCYSKYLRSELRDFVADLVHKCITIQT
                     GCLLQENEFSTLKVAGKTWQFLFHKKTFSIKQIDEQQAVENLNSFAKTKQNAKSENEK
                     KKEFPQQISEFASEGFLQFFFEDNVDNSFNVYVVDERNNVESYYNCFGNKEEKIRKIN
                     KLQNEKQLKTETPASFGSFNHPQFYQLISPNGELCIVPFQSRQHLDYLA"
BASE COUNT          418 a          182 c          197 g          355 t
ORIGIN      
        1 cagaaaaagc aattacattt attgaagcta aagttggatc tgcgttacaa cccggttggt
       61 atttagttaa tcaggcacct aaaagtcgtt atgactctga aaatcgttat gtccattata
      121 ataaaagccc gattaagctt attgcttggg ggtattttaa cggcattatt aacgcagcaa
      181 ctaatttaca tactgttagc cctaatttag atatcgagaa attgcgacaa tttatcaccg
      241 acttgcgctt atctttccca attcaagcgc cggaagtgac caatgatgac atgcatcacc
      301 ctaatgaaat tcgcaatttg gttatagcgc ttaatttagt acaagatcca accgaacata
      361 tcgatgaact accaaaaaaa gtttatccaa gcgatctgtt taattttggt aaggcacaaa
      421 aaacgttagt tggtagtgtg agcattattt atcgaaatgt atggaatgaa attcgaactc
      481 aacattttga cggtgaagat gctattttaa aagcactaaa attggtatcc aatcgaattt
      541 atcaaggaaa ttcttcacct caatcagtta atgttttttg ctatagtaaa tatctacgta
      601 gtgagttacg tgattttgtg gcagatttag ttcataaatg tattacaatt cagactggtt
      661 gtttactgca agagaatgaa tttagcactt taaaagtagc aggaaaaacc tggcagtttc
      721 tttttcataa aaaaacattt agcattaaac aaattgatga acaacaagcg gttgaaaatt
      781 taaattcttt tgcaaaaacc aagcaaaatg ctaaaagcga aaatgaaaag aaaaaagaat
      841 ttccgcagca aattagtgaa tttgccagtg aaggcttttt acagtttttc tttgaagaca
      901 atgtcgataa tagttttaat gtatatgttg tagatgaacg taataatgtc gaaagttact
      961 ataactgttt cggcaataaa gaagagaaaa tcaggaaaat caataaatta caaaatgaaa
     1021 aacaattaaa aacagaaaca ccagcatcat ttggaagctt taatcatcca caattttatc
     1081 aacttatttc accaaacggt gagttatgta ttgtgccgtt tcaaagccgc cagcatttag
     1141 attatttagc ac
//
LOCUS       DANLXF010000346         1149 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.347, whole genome shotgun sequence.
ACCESSION   DANLXF010000346 DANLXF010000000
VERSION     DANLXF010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.347"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..687
                     /gene="purU"
                     /locus_tag="PXD66_002526"
     CDS             <1..687
                     /gene="purU"
                     /locus_tag="PXD66_002526"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HDL6320763.1"
                     /translation="ELNGIFNDETLLTDLKFTLPEGSSYKLLPKQRKRIVILVTKEAH
                     CLGDLLMKNYYGGLDVEIAAVIGNHETLKSLVERFDIPFHLVSHENLTRVEHDKLLAE
                     KIDEYSPDYIVLAKYMRVLNPEFVARYPNRVVNIHHSFLPAFIGAKPYHRAYERGVKI
                     IGATAHFVNDELDEGPIIMQNVINVDHTYTAEAMMRAGRDVEKTVLSQALELVLADKV
                     FVYKNKTIIL"
     gene            complement(724..>1149)
                     /locus_tag="PXD66_002527"
     CDS             complement(724..>1149)
                     /locus_tag="PXD66_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL6320764.1"
                     /translation="YRLEAAKQLVQEGKVNQLLLSGDNKTPYYNEPKTMTSDLLKMGI
                     ATSKIKQDYAGYTTFYSIIRAKKVYKLPPFTIISQKFHCERALFIAKFRDIDAICYAA
                     TYPEGAYQVRMREILARSAMVFSLLIGKEPESLEDIRSK"
BASE COUNT          364 a          225 c          201 g          359 t
ORIGIN      
        1 gaattaaacg gaatttttaa tgatgaaacc ttactcactg atttaaaatt taccttacct
       61 gaaggctcaa gctataaatt actccctaaa caaagaaaac gtatcgttat tttagttacc
      121 aaagaagcac attgtttagg tgacttactg atgaaaaatt actatggtgg attagatgta
      181 gaaattgctg ccgtgattgg aaaccacgaa acattaaaaa gtttagttga acgtttcgat
      241 attccattcc atttagtcag ccacgaaaat ttaacccgag tggaacacga taaattatta
      301 gcggaaaaaa ttgatgaata ttcacctgat tatattgtgt tggctaaata tatgcgggtg
      361 ctgaatccgg aatttgtcgc tcgttatcca aaccgagttg tcaatattca ccactcgttc
      421 ttacccgctt tcattggtgc aaaaccttat catcgtgcat acgaacgtgg agtgaaaatt
      481 atcggtgcaa ccgcccattt tgtgaatgat gaattagatg aaggcccgat tattatgcaa
      541 aatgtgatta atgttgatca cacctacacc gcagaggcta tgatgcgtgc cggacgtgat
      601 gtcgagaaaa ccgtacttag ccaagcctta gaattagtgc ttgctgacaa agtatttgta
      661 tataaaaaca aaaccattat tttataagca aaatagccgg tcaacattat ctgaccggct
      721 atctcattta gaacggatat cttccaatga ttcaggctct ttacctatca acaaactaaa
      781 aaccattgcc gatctggcta aaatttcccg cattcgtacc tgataagccc cctctggata
      841 tgtagcggca taacaaattg catcaatatc cctaaatttt gcaataaaca atgccctttc
      901 acagtggaat ttttgggaga taatcgtaaa tggcggtaat ttatatactt ttttagccct
      961 aattattgaa taaaaagtgg tataccccgc ataatcctgc ttaatttttg aagtcgcaat
     1021 ccccatttta agtagatcac ttgtcattgt ttttggctca ttatagtacg gcgttttatt
     1081 atctccactt aacaaaagct gattgacctt tccttcttgg actaattgct tagcggcttc
     1141 caaacgata
//
LOCUS       DANLXF010000347         1135 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.348, whole genome shotgun sequence.
ACCESSION   DANLXF010000347 DANLXF010000000
VERSION     DANLXF010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.348"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            134..715
                     /locus_tag="PXD66_002528"
                     /pseudo
     CDS             134..715
                     /locus_tag="PXD66_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252416.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            716..886
                     /locus_tag="PXD66_002529"
     CDS             716..886
                     /locus_tag="PXD66_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HDL6320765.1"
                     /translation="MLKCKQITELVSLSQEQKLTLKQRVNVQVHLMLCSRCRAFSQNC
                     KTVDKLMKEFNS"
BASE COUNT          383 a          197 c          224 g          331 t
ORIGIN      
        1 caggacccgc ccctacgatt agtatgttag gtttaaaaat acttcatacc aatatcccct
       61 aagttgggta aaaccttatg aaataggata caagcggtta gtttctctca attttttacc
      121 aaaggatcac gcaatgagcc aaccggcttt acggcaaatt tccccctcgc agctagaaga
      181 tattcgtctg caaatggtga agtttgcctt tttacagtta aaaaataacg aacttgccga
      241 agatgtggtg caagaggcgt ttgtcagcgc ttataaatat gccgacagct tcaaaggtga
      301 aagtgcgtta aaaacgtaga tttttgcaat tttaaaaaac aagatcgtag atttagtgaa
      361 agccaaaggc aaattggtgg cagtatctga gctgatggaa gaagacgatc acgatcttgc
      421 cgataaattg tttaacgaaa atggcggttg ggataaagat gcttatcaat ctaatgagtg
      481 gaagaacacc gaaagccata cctattccgc tcaattctgg caagtatttg aattttgcct
      541 aaataatctg ccggcaaatc aagcaagggt gtttatgatg cgttcccatt tggaaatgga
      601 aaccgaagaa atttgcgcag aatgtgagat ttcaccagca aatgtgcata ccttactgta
      661 tcgggcaaga ttgcagttac aggtctgttt aagtcaaaaa tggtttggag gttaaatgtt
      721 aaaatgtaag caaattaccg aattagtttc tctgtcccaa gaacaaaaac tgacattaaa
      781 acaacgtgtt aatgtgcaag ttcatttaat gctttgttct cgttgcagag cctttagcca
      841 aaactgcaaa accgtagata agctaatgaa agaatttaac tcttagcgta aaagcaagcg
      901 gtaaaatttt taataaatat ttcaaactta agtgagcata aggcttatta taaataaaac
      961 gttccgaaaa aacattgaat ttcattaaaa aattccttta tttatcaaat aaatgcgatt
     1021 tgtttcggaa cgttttttaa tgcttatttt gcaacatacg gattatcaat ggaaagcgtt
     1081 gctgtagaca ttgtgcctcc tagtacaaaa aaaccgcttg gtttccctaa gcggt
//
LOCUS       DANLXF010000348         1120 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.349, whole genome shotgun sequence.
ACCESSION   DANLXF010000348 DANLXF010000000
VERSION     DANLXF010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.349"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..150
                     /locus_tag="PXD66_002530"
     CDS             <1..150
                     /locus_tag="PXD66_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HDL6320766.1"
                     /translation="LAQKKAKVWIYSYISPKTGKRSTKNRIGSYPQMSLVEQSPQVFE
                     QIARL"
     gene            202..789
                     /locus_tag="PXD66_002531"
     CDS             202..789
                     /locus_tag="PXD66_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SocA family protein"
                     /protein_id="HDL6320767.1"
                     /translation="MFDELKIAQATAYLLHKAGGTMEHIKVMKLLYLADRLSWQRYGY
                     SISNDEYYSLPYGPVLSNTLNLMRGEEASLYQKTSIWGEWIADKEDHKISLKKSVNTE
                     DEYFFDCLSESDTEILEEVFATFGNYKPFALVEYTHNPKYIPEWEDPQGRSKPISLST
                     LLTKLGKTPSEIESILEQQAEKGTMDNLFKGLGNA"
     gene            782..1099
                     /locus_tag="PXD66_002532"
     CDS             782..1099
                     /locus_tag="PXD66_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320768.1"
                     /translation="MLEIKRQVTILRPTNYGKEENHYKHLYILLTDPCFDGETGKGDM
                     ILSVNCSSIKDGKALDETCILKAGTHEFITRDSFIFYRHIKIDSLDEIKANIEAGYFI
                     QKP"
BASE COUNT          407 a          211 c          230 g          272 t
ORIGIN      
        1 cttgcacaga aaaaagcgaa agtatggatt tatagctata tcagccccaa aacaggcaag
       61 cgaagcacaa aaaatagaat cggtagctat ccgcaaatga gcttagtgga acaatcccca
      121 caggtatttg aacaaatcgc acgtctttaa aatattaaat taagagaaag cactatttgc
      181 gatgtagcag gagataaaac aatgtttgat gaattaaaaa tagcccaagc gaccgcctat
      241 ttgctacata aggcaggcgg aacaatggag catattaagg taatgaaatt gctttatctt
      301 gcggatcgct tatcgtggca aagatacggc tattctattt ctaatgatga atattattca
      361 ttgccttatg gaccggtatt aagcaacaca ctcaacctaa tgcgaggaga agaagcgagc
      421 ttataccaaa aaaccagtat ttggggcgag tggatagcag ataaggaaga tcacaaaatc
      481 agccttaaaa aatccgtaaa taccgaagat gaatacttct ttgattgcct ttcagagagc
      541 gacaccgaaa ttttagaaga ggtatttgcg acctttggaa attataagcc attcgcccta
      601 gtggaatata cccataaccc taaatacatt cccgaatggg aagacccaca aggcagaagc
      661 aagccgattt ctttaagcac gcttcttact aagctaggga aaacgccaag cgagatcgaa
      721 agcattttag agcaacaggc ggaaaaaggg acaatggata accttttcaa gggcttaggc
      781 aatgcttgaa atcaaaagac aagtaacgat tcttagaccg accaactacg gcaaggaaga
      841 gaaccactac aagcatttat atattctgct taccgatccg tgctttgatg gtgaaaccgg
      901 taaaggagat atgattttaa gtgtaaactg ctcaagcatt aaagacggga aagcacttga
      961 tgaaacttgt attttaaagg caggaacaca cgaatttatc acacgggaca gcttcatttt
     1021 ctaccgccat ataaaaattg attcgttaga tgagattaag gcaaatatag aagccggtta
     1081 ttttattcaa aagccctaat taatgatgaa gcctatcaac
//
LOCUS       DANLXF010000349         1093 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.350, whole genome shotgun sequence.
ACCESSION   DANLXF010000349 DANLXF010000000
VERSION     DANLXF010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.350"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..192
                     /locus_tag="PXD66_002533"
     CDS             <1..192
                     /locus_tag="PXD66_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6320769.1"
                     /translation="PQDGSVIDWDNIKNAKLTKYPLDNSEKRTSYLGCFTTDVSESYT
                     VDGESVRDIQLNALRKVNE"
     gene            192..560
                     /locus_tag="PXD66_002534"
     CDS             192..560
                     /locus_tag="PXD66_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320770.1"
                     /translation="MKITLNLGIEYNGKIRRDCTVRLLTMGGQCTAQEMIADLGFEEN
                     SEALSKREQTLVDMAYLSQQVTFDGIYQGNVTAQFLFDNLATEDYWQLIEATLTLRKK
                     RLENGVSQSESESPANPDLA"
     gene            590..778
                     /locus_tag="PXD66_002535"
     CDS             590..778
                     /locus_tag="PXD66_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320771.1"
                     /translation="MILLSKFAITAEQVWQMPLLEVFAWIESYLESEGVKTPHSPMGA
                     NSEVKRESYVFTRCGKIE"
     gene            831..>1093
                     /locus_tag="PXD66_002536"
     CDS             831..>1093
                     /locus_tag="PXD66_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320772.1"
                     /translation="MAKNMKVQLELTAKDTASPVLNQAAKTAEKAFKNTERAAQKSGN
                     AQTQAAQKAANATESSNRKIEQSYRNARRVAADVSRARETLGVR"
BASE COUNT          348 a          216 c          274 g          255 t
ORIGIN      
        1 cctcaagacg gttcggtaat tgattgggat aacattaaaa atgcgaagct cactaaatac
       61 ccgttagaca atagcgaaaa acgcacctct tatttaggct gttttactac cgatgtgagc
      121 gagtcttaca ccgttgatgg tgagtcggtg cgtgatattc aactcaacgc attgcgtaag
      181 gtgaatgaat aatgaaaatc actttaaatc ttggcattga atataacggg aaaattcgcc
      241 gtgattgtac ggtgcgattg ctgacgatgg gcggtcaatg cacggctcaa gaaatgattg
      301 cggatttagg gtttgaggaa aactcggaag ccctatcaaa gcgtgaacaa accttggtcg
      361 atatggctta cctttcgcag caagttacct ttgatggtat ttatcaaggt aatgtgacgg
      421 ctcaattttt atttgacaat ttagctaccg aagactactg gcagttgatt gaggcaacgc
      481 tcacactccg aaaaaagcgg ttagaaaatg gggtgagcca aagcgagagc gaaagcccgg
      541 caaatcccga tttggcgtag cagaagcctt taagcagtac cgacaagcgg tgattttact
      601 ctcaaaattt gcaattaccg ccgagcaggt atggcaaatg ccgttgcttg aggtatttgc
      661 gtggattgag agctacttgg agagtgaggg cgtaaaaaca ccgcatagcc caatgggtgc
      721 gaatagcgaa gtgaaacgcg aaagctatgt ctttactcgg tgtggcaaaa ttgaataggg
      781 gcgaaagccc cttttttgta ctttaaaatg agtttaaaga gactttaaaa atggcaaaaa
      841 atatgaaagt gcaactggaa ttgacggcaa aagatactgc ctccccagtt ttaaaccaag
      901 ccgctaaaac cgccgaaaaa gcctttaaaa atacagagcg agcggcacaa aaaagtggca
      961 atgctcaaac gcaagcagca cagaaagcag cgaatgctac agaaagctca aatcggaaaa
     1021 tagaacagag ttaccgtaat gcccgccgag tggctgccga tgtgagccga gcccgtgaaa
     1081 cattaggtgt tcg
//
LOCUS       DANLXF010000350         1084 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.351, whole genome shotgun sequence.
ACCESSION   DANLXF010000350 DANLXF010000000
VERSION     DANLXF010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.351"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..>1084
                     /locus_tag="PXD66_002537"
     CDS             <1..>1084
                     /locus_tag="PXD66_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HDL6320773.1"
                     /translation="TLVALAVTAFAASASATTVYDAEGSKVDFDGSLRIVLDRTNSKE
                     LDNHNVVKTKKNAHTGIRNTGSRFGVRVKHDLAQDFYALGRVEFRFNKNTSDDGFGDL
                     YTKRAYVGLGSKQYGELTFGRQVTIADDLSQSVDYNYGINPKGDYIQTDGNQVIRYNY
                     RGVENLHVSANYNFAQDRKDGKVLANKLQNAYGIGALYTVDNWDLRAAYGHTNYKTAT
                     DSTKHRKDGVLGSVGYKFGDLKLSVDTGYSREKGLAVTDDNGVTTFYNGVKSNAFYVS
                     PNFEYQVVPASKVYGNYLYERVKSKSNGSTYAAGKEKTHGFLLGVDYQFHKQVVAFVE
                     GSYTRTKDYDKVTNGYKYSGKSTNKAI"
BASE COUNT          362 a          164 c          211 g          347 t
ORIGIN      
        1 aacattagtt gcattagcag taaccgcgtt cgcagcgtca gcatcagcaa ccaccgttta
       61 cgatgcagaa ggttcaaaag tagattttga tggttcttta cgtattgttc ttgatagaac
      121 aaattctaaa gagctagaca atcataatgt tgttaaaact aaaaaaaatg cacacacagg
      181 aatccgtaac actggttcac gttttggtgt aagagtaaaa catgatttag ctcaagattt
      241 ctatgcatta ggtcgtgttg agttccgttt taacaaaaat accagtgatg atggttttgg
      301 tgatttatat actaaacgtg cttatgttgg tttaggtagt aagcaatatg gtgaattaac
      361 ttttggtcgt caagtaacga ttgctgatga tttaagtcaa tcagtagatt acaactacgg
      421 tattaatcca aaaggtgatt atattcaaac cgatggtaac caagttattc gttataacta
      481 ccgtggtgtt gaaaacttac atgtaagtgc aaactataac tttgcacaag atcgtaaaga
      541 tggtaaagta ttagcaaata aacttcaaaa tgcatacggc attggtgcat tatacactgt
      601 agataactgg gatttacgtg cagcttatgg tcatacaaac tacaaaacag ctactgatag
      661 cactaagcac cgtaaagatg gtgtattagg ttctgttggt tataaatttg gtgacttaaa
      721 attaagtgtt gatacaggtt attctcgtga gaagggttta gctgttacgg atgataatgg
      781 tgtaactaca ttctataatg gtgttaaatc aaacgctttc tatgtatctc caaactttga
      841 atatcaagta gtgcctgcat ctaaagtata tggtaactac ttatatgagc gtgtgaaatc
      901 taaatcaaat ggttctacat atgctgctgg taaagaaaaa actcatggtt tcttacttgg
      961 tgttgattac caattccata aacaagtagt tgcttttgtt gagggttctt atactcgtac
     1021 taaagactat gataaagtaa ctaatggtta taaatactca ggcaaatcaa ctaacaaagc
     1081 tatc
//
LOCUS       DANLXF010000351         1046 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.352, whole genome shotgun sequence.
ACCESSION   DANLXF010000351 DANLXF010000000
VERSION     DANLXF010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.352"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            43..>1046
                     /locus_tag="PXD66_002538"
     CDS             43..>1046
                     /locus_tag="PXD66_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HDL6320774.1"
                     /translation="MADSVKSHEQSSDEIDLIEIFRALWDKKWWIVITTFITTLLAGI
                     YAFTAKEQWTSKATVIAPKLTELGEYLNVRREYSRILRVDPVDPNALSASLFNNFNRL
                     AQSQDTKNEFFITSNVYKTLAEGKDEQGQRTVLSNLSNENTSFIIPDAKKDPNAIGRT
                     LSFSAETPMEAQDTLGKLVQFINSKAFAVELEDFLVDFQNVLTALQYEKSLIQEDLSV
                     NKSVQLENLNKAYETAQKAGIKEYSKAFNQSDASNIAAALSDTKISLSDSKLADSSYL
                     FMLGEKYLKAQIDVANEKGVVYPPRYYQIDYQLKQLEPLFEKTKTVKTQTFSYQASPD
                     "
BASE COUNT          367 a          164 c          194 g          321 t
ORIGIN      
        1 tgtataatgc tgtacagaaa catgcaaatt ttagaggaaa ctatggcaga ttcagtaaaa
       61 agtcatgagc aaagctcgga tgagattgat ttaattgaga tttttcgtgc tctatgggat
      121 aaaaaatggt ggattgttat aacaacattc attaccacat tacttgctgg tatttatgca
      181 tttactgcaa aagagcaatg gacgtctaag gcaacagtta ttgcccctaa attaacagag
      241 ttaggtgagt atttaaatgt tcgcagagaa tatagtcgga ttttgcgtgt agatcctgtt
      301 gatccaaatg cattgtctgc gagtttgttc aacaatttta accgattagc acaatctcaa
      361 gatactaaaa atgaattttt tatcacttcc aatgtgtata agacgttagc tgaaggaaaa
      421 gatgagcagg ggcaacgcac agttttatcc aatttgagta atgaaaatac cagctttatt
      481 attcctgatg caaagaaaga tcctaatgca ataggtcgta cactatcttt ttctgcagag
      541 acacctatgg aggcgcagga cacccttggt aagttggttc aattcattaa tagcaaagca
      601 tttgctgttg aattagaaga ttttctagtc gatttccaaa atgtgcttac tgcattacaa
      661 tatgaaaaat cattaattca ggaagattta tctgtgaata agtcggttca gttggaaaac
      721 ttaaataaag cctatgaaac tgcacaaaaa gcaggtatta aagaatattc aaaagcattt
      781 aatcaatcag atgcatcaaa tattgcagca gctttaagtg atactaaaat ctccctttct
      841 gattcaaaat tagcagatag ttcatattta tttatgttag gggaaaaata cctaaaggct
      901 caaattgatg tggctaatga gaaaggtgtt gtttatccac cacgttatta tcaaattgat
      961 taccaactta aacagctaga gccgttattt gaaaaaacaa agacagttaa aacacaaact
     1021 tttagttacc aagcttcgcc ggatta
//
LOCUS       DANLXF010000352         1035 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.353, whole genome shotgun sequence.
ACCESSION   DANLXF010000352 DANLXF010000000
VERSION     DANLXF010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.353"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..807
                     /locus_tag="PXD66_002539"
     CDS             <1..807
                     /locus_tag="PXD66_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL6320775.1"
                     /translation="VEEFTETLKTLSRDDLSAEERTTKTIDSVKLRIYLNEQARNRKN
                     YINGFVPTDWIPEITDQMTKSKNLWLKYEEEAKTTLKPYQEETISRVIQDEINKKMAE
                     IGTTTLEIGGKEHELEDLENQINALALTDEEKLAEDVENDLISKLDEAKAALAKNQQT
                     LKEKRDELLGLNNKLDSSPLVKKGKNALAEGTNAFASGENAIAFGTDSQATGNNAIAL
                     GANSKANAESAIAIGKGAQALKEKALALGENAIANRASAIAIGDNHSSKL"
     gene            complement(856..>1035)
                     /locus_tag="PXD66_002540"
     CDS             complement(856..>1035)
                     /locus_tag="PXD66_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HDL6320776.1"
                     /translation="RKKIYTDLTTLQADLDEWLMYYNHHRTHQGKMCCGRTPMATLLD
                     GKRIWVEKEFKLKLI"
BASE COUNT          388 a          189 c          186 g          272 t
ORIGIN      
        1 gtagaggaat ttactgaaac attaaaaact ttatctagag atgacttaag tgcagaggaa
       61 cgtacaacca aaacaattga ctccgttaaa ttaagaatat atctcaatga acaagcacga
      121 aatcgtaaaa attatattaa tggatttgta cctaccgatt ggattcctga aattacagat
      181 caaatgacaa aatccaaaaa tctatggtta aaatatgaag aggaagccaa aacaacatta
      241 aaaccatacc aagaagaaac aatatctagg gttatacaag atgaaattaa taaaaaaatg
      301 gctgaaatag gtactacaac actagagatt ggtggtaaag aacacgagct cgaggatctt
      361 gaaaaccaaa ttaatgcttt agctttaact gatgaagaaa aacttgcgga agatgtggaa
      421 aatgatttaa tctcaaagct tgatgaggct aaagcagcct tagcaaaaaa ccagcaaacg
      481 ctcaaagaaa agcgtgatga attactaggg ctaaataaca aattagatag ctcaccactt
      541 gttaaaaaag gtaaaaatgc acttgcagaa ggcacgaatg cctttgcttc tggtgaaaat
      601 gccatcgcat tcggcacgga ttcacaagct acaggtaata acgctatcgc actaggtgca
      661 aattcaaaag caaacgcaga aagtgcgatt gcaattggta aaggtgcaca agcattaaaa
      721 gaaaaagccc ttgctttagg cgaaaatgcc attgccaaca gagcatctgc tattgcgatt
      781 ggtgataacc attcatctaa attgtagaag atcaaaccta atctgacagt cccccgtttt
      841 aaattaccgt gtctgtcaga ttaatttgag cttaaattct ttttccaccc aaatccgttt
      901 tccatcaagt aaggttgcca tcggtgttct gccacagcac atttttcctt gatgtgttcg
      961 atggtgatta taatacatta accactcatc taaatccgct tgtaatgtcg ttaaatccgt
     1021 ataaattttc ttcct
//
LOCUS       DANLXF010000353         1023 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.354, whole genome shotgun sequence.
ACCESSION   DANLXF010000353 DANLXF010000000
VERSION     DANLXF010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.354"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..986
                     /gene="sohB"
                     /locus_tag="PXD66_002541"
     CDS             <1..986
                     /gene="sohB"
                     /locus_tag="PXD66_002541"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HDL6320777.1"
                     /translation="AVVVMLILESKKQNENGSISITNLSEKYKEQQKSLSGFFLSEEE
                     LKHQEKQEKKVAKEKAKAEKKRLKAGEECVESPSRLFVLNFNGDMMAHEVNSLRREID
                     AVISLANPEKDEVLLKLESPGGVVHGYGLAASQLQRLRDRNIYLTAAVDKVAASGGYM
                     MACVANKIVSAPFAVIGSVGVVAQVPNIHRLLKKHDIDVDVMTAGEYKRTVTLVGENT
                     EKGKQKFQQELEETHSLFKQFVARHRPQLDIEQVATGEHWFGQQAIELNLVDEIATSD
                     DLLLNAIKEKEVIELKYKEKKKLAQKLGSQFEQSAENLLAKILHKSRSTLM"
BASE COUNT          335 a          174 c          256 g          258 t
ORIGIN      
        1 ttgcagtggt ggtgatgctg attttggaat cgaaaaagca gaatgaaaac ggctcaattt
       61 caattaccaa tttatctgag aaatataaag agcagcaaaa atctctttcc ggcttttttc
      121 taagtgagga agaattaaaa catcaggaaa agcaggagaa aaaagtagca aaagagaaag
      181 ctaaagcaga aaaaaaacgc ttaaaagcag gcgaagaatg tgtagaaagc ccttcccgtt
      241 tatttgtatt gaattttaat ggcgatatga tggctcacga agtaaattcc ctacgccgtg
      301 aaattgatgc ggtaattagc ctcgcgaatc cggaaaaaga tgaagtttta cttaaacttg
      361 aaagtccggg cggagtggtg cacggttatg gattggctgc ttcgcagtta caacgtttgc
      421 gtgatcgcaa tatttattta accgcagcgg tagataaagt agctgccagc ggtggttata
      481 tgatggcgtg tgtggcgaat aaaattgtct ctgccccatt tgcggtgatt ggctcagtgg
      541 gggtggtggc tcaggtgccg aatatccacc gcttactgaa aaaacacgat attgatgtgg
      601 atgtgatgac tgcaggtgaa tataaacgca cggtgacgct ggtgggagaa aataccgaaa
      661 aagggaaaca gaaattccag caagaactgg aagaaacgca ctctctgttt aagcaatttg
      721 tggctcgaca ccgcccacaa ctggatattg agcaggttgc aacaggcgag cattggtttg
      781 gtcagcaagc gattgagcta aatttggttg atgagattgc aaccagtgat gatctgctcc
      841 tcaatgcaat taaggaaaaa gaagtgatcg agctaaaata taaagagaag aaaaaacttg
      901 ctcagaaatt gggttctcaa ttcgagcaat cagcagagaa cctgctcgct aagattttac
      961 ataaaagtcg ttcaacgctg atgtagcgta tatattatta aagggctatc gtttatatag
     1021 ccc
//
LOCUS       DANLXF010000354         1022 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.355, whole genome shotgun sequence.
ACCESSION   DANLXF010000354 DANLXF010000000
VERSION     DANLXF010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.355"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            11..463
                     /locus_tag="PXD66_002542"
     CDS             11..463
                     /locus_tag="PXD66_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDL6320778.1"
                     /translation="MKPFDLNKALAGEPVKLRNNDKAFVKYLISDDYIRDNKDHQVQG
                     YTVDEENVFLSEVSWAVSGSHFNDGTIAQYDIVGMWEEPRPAVTLTLPCPLKEPQENM
                     WFIDNDFTIVKSMFANAPFVKKFLPQGRCFASEEDAQEWLDAMRNSRR"
     gene            541..>1022
                     /locus_tag="PXD66_002543"
     CDS             541..>1022
                     /locus_tag="PXD66_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HDL6320779.1"
                     /translation="MNEEKYFSVDVSDETHVIRLHETLEQAKQSCLNGATEAYEFAGD
                     MDDHESYESYEAYDLPYAVYGVVLGRAKSDIRPLTDEERASELFGEAEQVIEPPTLLE
                     NNGWISIEDKLPPIETDVLGLCDISGMQLILIVSRELADNEWYFLSVNQYGLDDDVIA
                     V"
BASE COUNT          358 a          155 c          228 g          281 t
ORIGIN      
        1 ggagaataaa atgaaaccat ttgatttaaa taaagcattg gcaggtgagc cagtaaaact
       61 aagaaataat gataaggcat ttgtaaaata cctaattagt gatgactata ttagagataa
      121 taaagaccac caagtacaag ggtatacagt tgatgaagaa aatgtatttt tgtcagaagt
      181 atcgtgggct gttagcggat ctcatttcaa tgatggaaca atagctcaat atgatattgt
      241 tggtatgtgg gaagaaccaa gaccagcagt aacattgacg ttaccttgcc cattaaaaga
      301 acctcaagaa aatatgtggt ttattgacaa tgattttacc atagtaaaat ctatgttcgc
      361 aaatgccccc tttgtaaaaa aattcttgcc acaagggcga tgtttcgctt cagaagaaga
      421 cgctcaagaa tggttagatg caatgcgaaa tagtcgtagg taaatcggca gcactaacag
      481 ctgaatgctg ctgttgaatt tcttgtatat ccacataaac acggaacgcg gagaaagaaa
      541 atgaatgaag aaaaatattt ttcggtcgat gtctcggatg agacacatgt aataaggttg
      601 cacgaaacat tagagcaagc aaaacaaagc tgtttaaatg gtgctactga agcctatgag
      661 tttgcgggtg acatggatga tcacgaaagc tatgaaagct atgaagccta tgatttaccc
      721 tatgcagttt atggtgttgt tttaggtaga gcaaaatcag atattcgtcc actaacagac
      781 gaagaaagag catcggagtt atttggggaa gctgaacaag ttattgaacc accaacactt
      841 ttagaaaaca acggctggat ttccattgag gacaaattac cacctattga aacggatgtg
      901 ctagggttat gcgatatttc aggaatgcag ttaattttaa tagtttcaag agaacttgcc
      961 gataacgagt ggtattttct tagtgtaaat caatatgggc ttgatgacga tgtgattgca
     1021 gt
//
LOCUS       DANLXF010000355         1017 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.356, whole genome shotgun sequence.
ACCESSION   DANLXF010000355 DANLXF010000000
VERSION     DANLXF010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.356"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            1..952
                     /locus_tag="PXD66_002544"
                     /pseudo
     CDS             1..952
                     /locus_tag="PXD66_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248924.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
BASE COUNT          329 a          195 c          228 g          265 t
ORIGIN      
        1 tcatcccgtg ctaggaggat tccttgcaaa tcagctcatt cccaagtctg cgtttgattg
       61 gctgggacag tatccagaag tcatttcttc actgatgtat gcaatgtctg ggcataacga
      121 taaagccggg cttttatctg agatcgtgca gaaagccgat cagcatagtg ttacgctagc
      181 aatcggtggc gatgtgaata agctggtaca gcgaccagtt aattcattgc cgaagcaaat
      241 tgtgctggct ctacgttatt tactggagca gaaaatcaaa ttaaatacgc caaaaggacc
      301 agcagacggc tggtttaccg aagatgggct gtggctggta agtaaaactg ctgccgataa
      361 tatccgaact tatttactgg gacaggggat ttccgtgcct aaagataatc caaaactgtt
      421 tgatgaaatg caatctcttg gtattgtgga agagtctcct tccggcggtg caatttggag
      481 ctgccgtatc caagccgatt ccggttggac accggcgaca acatttacgc tattacgagt
      541 taaaccggag gttgcttggg agaatatcaa cgatagaccg gcgtattttg ccggaaaagt
      601 caaagtgatt gaacagataa ctgaagaggt caatgcagag gttcagcctc cattagttga
      661 agaaaccact aaaatttctc tattaccgag caagcaatcg gaggctatta ttccgtctga
      721 ggaaacggaa atatcgaagg caattacaag ttcagacaat gcaacatctg cggaagtaat
      781 cataaataat gaaaatgatt taacttcttt tgtattggat atgtttgctc ctacagagac
      841 aattgagcaa aatatttctg tcatttcttc aaataaagag cagcaaatcg taatgacaac
      901 acttacggaa caagaaaaag ttccgccgaa aaaagttaaa ttagagaaaa gtaagaaaaa
      961 aaatcttcaa ttaaccactc tagaaccatg gaatgatatg attaatccaa ttactgg
//
LOCUS       DANLXF010000356          980 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.357, whole genome shotgun sequence.
ACCESSION   DANLXF010000356 DANLXF010000000
VERSION     DANLXF010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.357"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..>980)
                     /locus_tag="PXD66_002545"
     CDS             complement(<1..>980)
                     /locus_tag="PXD66_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL6320780.1"
                     /translation="FKLFPLNKANFESQIKGSLSLGLFIESCWKGNFGAWEYAFTSPN
                     LQHQNAQNLLKALDVCKNRNFPIVFWNKEDPMHYEKFLPISSKCDVIFTTDSNKVKDY
                     QRDVPNAKVETLMFAANINICNPANRFRYEAENICFAGSYYGVGHDDRKKQMDALLPT
                     IIKFKGAIYDRMSQVQSADERYSYPKQYRKFIRPAVPFKEIVDIYKQFKIFLNVNTIT
                     DSPTMMSRRVYELLACGTPVISTPSLAIDEQFKGIVQVAKDAKEANKIAKRLLENEWE
                     WLRISHLGYREVMLKHTYEHRAVQIANTLGQDIKPEAPLASIVVASNRPY"
BASE COUNT          320 a          181 c          145 g          334 t
ORIGIN      
        1 atatggtcga ttagatgcaa caacaatact tgccaatggg gcttcaggct taatatcctg
       61 ccctaatgta tttgctattt gtacagctct atgctcataa gtatgtttta acattacctc
      121 tcgatagcct aaatgagaaa ttcttagcca ctcccattca ttttctaaca atctcttagc
      181 aattttattg gcttctttgg catctttagc tacttgtaca atacctttaa attgttcatc
      241 tattgctaat gatggtgtag aaattacagg tgtaccgcat gccaataatt catacactct
      301 gcgtgacatc attgtaggag aatcggtaat agtattaaca tttaaaaata ttttaaattg
      361 tttataaata tcaactattt ccttaaatgg gactgccggt ctgataaatt tgcggtattg
      421 tttaggataa gaataacgct catcagctga ctgtacctga gacatacggt cataaatagc
      481 tcctttaaat ttgataatag taggcaaaag agcatccatt tgcttcttac gatcatcatg
      541 accaacacca taataagaac cagcaaaaca gatattttca gcttcatatc ggaaacggtt
      601 tgctggatta caaatattga tatttgcagc aaacattaat gtttccactt tagcattagg
      661 aacatctctt tgataatctt tcactttgtt gctatctgta gtaaaaatca catcgcattt
      721 agatgaaatt ggtaaaaact tctcataatg catcggatct tctttattcc aaaatacaat
      781 aggaaaattt ctatttttgc aaacatcaag agcttttagt agattttgag cattttgatg
      841 ctgtaaattt ggcgaagtaa aggcatactc ccaagcacca aaattccctt tccaacaact
      901 ctcaataaac aaccctaatg ataaagaacc tttaatttga ctctcaaaat ttgctttatt
      961 taacggaaag agtttaaatt
//
LOCUS       DANLXF010000357          979 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.358, whole genome shotgun sequence.
ACCESSION   DANLXF010000357 DANLXF010000000
VERSION     DANLXF010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.358"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            134..820
                     /locus_tag="PXD66_002546"
     CDS             134..820
                     /locus_tag="PXD66_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320781.1"
                     /translation="MAANSINASHIVGSSITADKLNVNNLAAISANLGKVTAGTITGT
                     TISGGTISGTTITGGIIKGARIEGLTGKFTGELEINQLIGGNIYEAKTWTSKATGKSK
                     TYSETKNSFALSDTYYEYVCQFVLPATKATRTLYVVHTPTGESKSETFYGSGSNRTPT
                     TITLPSVIDISNTISPNVSNPFNDSGAILIANTQYVVTCRAWCAGRGRSAKVNFRAII
                     SSTNGSSYQL"
BASE COUNT          344 a          178 c          202 g          255 t
ORIGIN      
        1 cccttggcaa aatgccggtg taaccgctat ccacggtggc tcaatagtta ctaataccgt
       61 tactgctcaa caaattgcag caaataccat taccgccaac gaaatcgcag ccggcacgat
      121 tgcagctcga aatatggcag caaactcgat taatgcaagt catattgtgg ggagttcgat
      181 cactgcggat aagctaaatg taaacaattt agcggcaata tcggcaaatc ttggtaaggt
      241 aactgctggt acaattacag ggacgacaat tagtggtggg acaatttcag gtacaacaat
      301 tacaggggga ataattaaag gagcaagaat tgagggatta acaggtaaat ttactggaga
      361 acttgaaatc aaccaattga tcggaggtaa tatctatgag gctaaaactt ggacatcaaa
      421 agcaacaggg aaaagtaaaa catattcaga aacgaaaaac agttttgcat taagtgatac
      481 atattatgag tatgtatgtc aatttgtact acctgctaca aaagcgacga gaacattgta
      541 tgttgtccac actccaacag gagagagtaa aagcgaaact ttttatggta gtggtagtaa
      601 tcggacaccg acaacaatta cattaccgtc tgtaattgat attagcaata ctatttcacc
      661 taatgtatct aacccattta atgattctgg ggcaatactc attgctaata ctcaatatgt
      721 agtaacgtgt cgtgcgtggt gtgcagggcg tgggagaagt gctaaagtga attttcgagc
      781 aattatatca tctacaaatg gttcatctta tcaattatag gaggcaaata tgaaaaaaat
      841 tatactactt acaacaatta tggtttcagc ttgtacaact caatataagc aagactatac
      901 aacaaaacca accttttgtt atcaactagc ccctcaagag caattaccgg ggaaaaactg
      961 tattggctca ggagggcat
//
LOCUS       DANLXF010000358          966 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.359, whole genome shotgun sequence.
ACCESSION   DANLXF010000358 DANLXF010000000
VERSION     DANLXF010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.359"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            93..689
                     /locus_tag="PXD66_002547"
     CDS             93..689
                     /locus_tag="PXD66_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320782.1"
                     /translation="MKKLLMTLCCLPAFALANETGESCSKIEDSAKRLECYDSVFVKK
                     DTGKDEATDEKSNWEYEQKKDELRNATTYFAKIRSTNTIDFGFPYNSSSMNLMLRKDP
                     KYGNDVIFSVHGQFNGCMIESCKITVKFDDGKLESYRMIGADGGSNDTLFIENAKAMK
                     TFVDKLKKSKKLIVEASFYNYGKGQFTFDTQGLEWKHF"
     gene            complement(725..>966)
                     /locus_tag="PXD66_002548"
     CDS             complement(725..>966)
                     /locus_tag="PXD66_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320783.1"
                     /translation="FRNRLYHQEVVWNKRIAKKPTHALENLEKTYKQFELALQKIAPE
                     RFAFRQLSQALIWQRQIFFDQQIFEAEIVILPQHI"
BASE COUNT          328 a          134 c          203 g          301 t
ORIGIN      
        1 ccattaaaat ataattattg agattgcgag cttgatcaca gaccgaaaac cacgcttttg
       61 agaaaataag aagggatttc tatggaggtt atatgaaaaa actactgatg actttatgtt
      121 gtttgcccgc ttttgcttta gctaacgaaa ctggagaaag ttgctctaag attgaagata
      181 gtgcaaaacg cttagaatgt tatgatagtg tctttgtgaa aaaagatact ggtaaagatg
      241 aagctaccga tgaaaagtct aattgggaat atgaacaaaa gaaagatgaa ttgcgtaatg
      301 caacgactta ctttgctaaa attagatcaa ctaacacaat agattttggt ttcccgtata
      361 attcatcatc aatgaattta atgttacgca aagaccctaa atatggcaat gatgtaattt
      421 ttagcgtaca cggtcaattt aatggctgta tgattgagag ctgtaaaatt actgtgaagt
      481 ttgatgatgg taaattggaa agttatcgaa tgattggggc ggatggtggc agcaatgata
      541 cgcttttcat tgaaaatgca aaagcaatga aaacctttgt agataagttg aaaaagtcga
      601 aaaaactaat tgtagaggcg agtttttaca attatggcaa agggcaattt acctttgata
      661 cacaagggtt agagtggaaa catttttaat taagaaaaag ctacatagat gcgtagcttt
      721 ttgcttagat atgttggggc aaaataacga tttcagcttc aaaaatttgt tggtcgaaaa
      781 agatttggcg ttgccaaata agtgcttgtg aaagctgacg aaacgcaaat cgttcaggtg
      841 ctatcttttg tagtgctaac tcaaattgct tgtaagtttt ttctaaattc tctagtgcgt
      901 gcgttggttt cttggcaatt cgcttattcc aaaccacttc ttgatgatac agccgatttc
      961 taaaac
//
LOCUS       DANLXF010000359          944 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.360, whole genome shotgun sequence.
ACCESSION   DANLXF010000359 DANLXF010000000
VERSION     DANLXF010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..944
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.360"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            100..345
                     /locus_tag="PXD66_002549"
     CDS             100..345
                     /locus_tag="PXD66_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320784.1"
                     /translation="MTNDGSIIARGSGFTTYALELENDGLKYLVGFVQPNGNAKEFIE
                     PGATIRRSNDQPSIGSVDCDPRKKIHYKFDVHLMNTL"
     gene            564..>944
                     /locus_tag="PXD66_002550"
     CDS             564..>944
                     /locus_tag="PXD66_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4852 domain-containing protein"
                     /protein_id="HDL6320785.1"
                     /translation="MCKKAFPLWGLIFGLLLSTSAFSVSKNFNYQNILMESLHFSENI
                     ELSSLSKDWLLYSDRNTYHKYQNDEFEFQDKIEQAGKKLQKLVDDRKSEEAPIVFTIP
                     VNSEFGNYDFEQGKFEYQPLMNGHM"
BASE COUNT          323 a          159 c          187 g          275 t
ORIGIN      
        1 accagcacag aaggtaaacc tctcacagtt aaacgggtag gaaatgatta cgagtattcg
       61 tatgacaaaa ctactttcaa aaacccgatt aaaaaggcag tgaccaacga cggttcgatt
      121 atcgccagag gctcaggttt tactacttat gccttagagt tggaaaatga tggcttgaaa
      181 tatctagtcg gatttgttca accaaatgga aatgcaaaag agtttattga gccgggagca
      241 acaattcgcc gtagtaatga tcaaccaagt attggttctg tggattgtga ccctcgtaaa
      301 aaaatccatt ataagtttga tgttcatttg atgaatactt tataatacct ttgtggtgct
      361 agagctagcc caagttaaaa aagacaaagc tcggcagatg aacaattgtg gtgtcagagc
      421 tgacccacgt taaaaaacac aatgctcggt aggtaaatcc cttaggttta aaaaatctaa
      481 gggatttttg tttaaaagac ttgctcttta ttatcaggtg gatctatcat aatttaaact
      541 ctctaatgtg tcaggagata aaaatgtgta aaaaagcttt cccattatgg ggcttaatct
      601 tcggattact gctatccact tcagcattct cagtgtctaa aaacttcaac tatcaaaaca
      661 tattgatgga atcattacat tttagtgaga atatcgagct tagttcgtta tctaaagact
      721 ggttacttta cagcgataga aatacgtatc ataaatatca aaatgacgag tttgagtttc
      781 aggataaaat tgagcaagct ggtaagaaac tacaaaaact ggtggatgac agaaaatctg
      841 aagaagcccc tattgttttc acaattcctg taaactcaga attcgggaat tatgattttg
      901 aacaaggtaa gtttgagtac caacccctga tgaatgggca tatg
//
LOCUS       DANLXF010000360          940 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.361, whole genome shotgun sequence.
ACCESSION   DANLXF010000360 DANLXF010000000
VERSION     DANLXF010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.361"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..515
                     /locus_tag="PXD66_002551"
     CDS             <1..515
                     /locus_tag="PXD66_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase family
                     protein"
                     /protein_id="HDL6320786.1"
                     /translation="ANTLWQEKKIRLLLNPQGSMREISTQELSELLSMIDKRYVQHIS
                     CLLTNTTNSAEYLSKIKAPFEISLAPNSDILGYCALVNSADIVIAVDGGGVHISCALG
                     KKLLSFYANHPANIARWYPKPKKDVETFMVIADKWTEDNNDTRGFSLVAPAKWLNHQF
                     AKLIEKYTAC"
     gene            complement(570..782)
                     /gene="rpmE"
                     /locus_tag="PXD66_002552"
     CDS             complement(570..782)
                     /gene="rpmE"
                     /locus_tag="PXD66_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HDL6320787.1"
                     /translation="MKQGIHPEYKEITATCSCGNVVKTRSTVGKDLNLDVCGNCHPFY
                     TGKQRVVDTGGRVERFNKRFSIPSSK"
BASE COUNT          306 a          167 c          177 g          290 t
ORIGIN      
        1 ttgcaaatac gctatggcaa gagaagaaaa ttcgattact attgaatcct caaggtagta
       61 tgcgtgaaat ttctactcaa gaattatcag agctattatc tatgattgat aaaagatatg
      121 ttcaacatat tagttgtttg ctgaccaata caaccaatag tgccgaatat ttgtcgaaaa
      181 ttaaagcccc atttgaaata agcctagcac ctaatagtga tattttaggt tattgtgcat
      241 tagtaaactc tgctgatatt gtaattgcag ttgatggagg tggtgtccat atttcttgtg
      301 cattaggcaa gaaattgctc tcattttatg ccaatcatcc agctaatatt gcacgctggt
      361 atccaaaacc taagaaagat gttgaaactt ttatggtgat cgcagataaa tggaccgagg
      421 ataataatga tactcgaggt ttttctctgg tagctcctgc aaaatggtta aatcaccaat
      481 ttgcaaaatt gatagaaaaa tacaccgctt gttaattcga tatataagaa aaacaaaacc
      541 ccgcattagc ggggtttgca ataatcaaat tattttgagc ttgggatgct gaaacgtttg
      601 ttaaagcgtt caacacgacc accggtgtca acaacacgtt gtttaccagt atagaacggg
      661 tggcagttgc cacatacgtc taagtttaaa tctttaccca ctgttgagcg agttttaact
      721 acattaccgc aagagcaagt tgccgtaatt tctttatatt ctgggtgaat accttgtttc
      781 attggggaac ctcataagaa gccatatcgc cgcctatctt gttagaatct ctaccgctga
      841 taggtaccat atgtaattaa taaaaatgtt gagcaaatgc tcaacgccaa aaagagtgcg
      901 tattttagat aaatttgttg cctagatcaa ctactaaaca
//
LOCUS       DANLXF010000361          904 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.362, whole genome shotgun sequence.
ACCESSION   DANLXF010000361 DANLXF010000000
VERSION     DANLXF010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.362"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            5..895
                     /locus_tag="PXD66_002553"
     CDS             5..895
                     /locus_tag="PXD66_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL6320788.1"
                     /translation="MDKLNAISLFCKVIETQSFTQAAQQAQISLPMASKLVAQLEEHL
                     NVRLLQRTTRKITPTEAGLLYYQQCLPILNELKEADASVSNINSTLQGKITMSVPMDF
                     GSRFIAPYLGKFMEDYPNLALHIEFSDRRVDVIAEGFDLVLRIGSLADSSIVAKKIAD
                     SHLVLVASPDYLARFGEPKGLEALVERDCLLYEYHPQWQFSQQGQKMQIKPQGKIYSN
                     NGYALVQMAKAGLGIINIPKFLIKHELKNGELIPIMPFLTQEPLQISLLYPNRRYLSP
                     KVRILIDFFTQLMQEQKEYI"
BASE COUNT          302 a          176 c          166 g          260 t
ORIGIN      
        1 gaaaatggat aaactaaacg caatcagttt attttgtaaa gtaattgaaa cccaaagttt
       61 cactcaagca gcacaacaag ctcaaatttc gttacccatg gcaagtaaat tggtggctca
      121 gttagaagaa catctcaatg tgcgtttact acaacgaacc actcgaaaaa ttacgccaac
      181 tgaggcaggg ttgctctact atcagcaatg cttgcctatt ttaaatgaac taaaagaggc
      241 cgatgccagc gtaagtaata ttaactcaac gctacaaggg aaaataacaa tgtctgtacc
      301 aatggatttt ggctctcgct tcattgcccc ttaccttggg aaatttatgg aagattaccc
      361 taatttagcc cttcatattg aatttagtga ccgtcgggta gatgtgattg cagaaggctt
      421 tgatttggtg ctgcgtattg gtagccttgc agatagttcc attgtagcga aaaaaattgc
      481 agattcccat ttggttttag ttgcttctcc agattattta gctcgatttg gcgaacctaa
      541 aggcttagaa gcattagtgg aacgagactg cctactctat gaataccatc ctcaatggca
      601 attttctcaa caaggacaaa aaatgcaaat taaaccacaa ggtaaaattt acagtaacaa
      661 tggttatgcg ttagtacaaa tggcaaaagc tggtttgggc atcattaaca ttccaaagtt
      721 tttaataaaa cacgagctaa aaaacggaga gttgattcct attatgcctt ttttgacaca
      781 ggaaccttta caaatcagct tactctatcc aaaccgtcgt tatctctctc ctaaagtgcg
      841 aatcctaatt gattttttca cacaactgat gcaagagcaa aaagagtata tttaacaaaa
      901 aaga
//
LOCUS       DANLXF010000362          900 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.363, whole genome shotgun sequence.
ACCESSION   DANLXF010000362 DANLXF010000000
VERSION     DANLXF010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.363"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            38..511
                     /locus_tag="PXD66_002554"
     CDS             38..511
                     /locus_tag="PXD66_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Type II secretory pathway, pseudopilin PulG"
                     /protein_id="HDL6320789.1"
                     /translation="MVIAVYFISPVIFTLQDRIALHSEIENIQSFIQQIQTKARYTKR
                     NYTLTLSQRNNQWCMIAVIKPINTSKQIICDCLNKHFCDLGKEYHLYTPTHQQIVLKN
                     KSLYPKSFINVDGVSGQLESKCIKLSLNKENEILQFEQLGRVYVAAKNKRSTCKD"
     gene            543..>900
                     /locus_tag="PXD66_002555"
     CDS             543..>900
                     /locus_tag="PXD66_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320790.1"
                     /translation="MASALGAFLLLLVASSYADFYYTQTKQKEQLYLQAEAHQLLNYF
                     QQHFQHIGYQGSHRTDSNFDLFQQAGKSLNIVHKNCIIGFYDLNQDGCLGKRKTKTAA
                     CKLGTVNNTREVLKEIV"
BASE COUNT          318 a          146 c          154 g          282 t
ORIGIN      
        1 catcacatta attgaaatac taaccactat cactattatg gtgatcgctg tttattttat
       61 ctcgccggtt atttttacac tgcaagatcg aattgcactt catagtgaaa ttgaaaatat
      121 ccaatctttt attcagcaaa tccaaactaa agcacgttat accaaacgta attatacctt
      181 aaccctttct cagcggaata atcagtggtg tatgattgcg gtaattaagc caatcaatac
      241 tagcaaacaa attatttgtg attgtttgaa taaacatttt tgtgatttag gaaaagaata
      301 tcatctttat acgccgactc atcagcagat tgtattaaaa aataaaagtt tatatccgaa
      361 atcctttatt aatgttgatg gggtaagtgg tcaattagaa tcaaaatgta ttaagctcag
      421 tttaaataaa gaaaatgaaa ttttgcaatt tgaacaatta ggacgggttt atgtggcagc
      481 aaaaaataaa cgtagcacct gcaaagatta gtttatctgc ttttagccta attgaagtat
      541 taatggctag tgcattaggg gcgtttttat tattgcttgt tgcttcaagt tatgccgatt
      601 tctattacac acaaaccaaa caaaaagaac agctctactt gcaagcagaa gctcaccagt
      661 tactaaacta cttccaacag cattttcaac acattggcta tcaaggctct cacagaacag
      721 acagtaattt tgatttattt cagcaagcag ggaaaagcct gaatattgtg cataaaaatt
      781 gcattattgg attttacgat ttaaaccaag atggctgttt gggaaaacgt aagacaaaaa
      841 cggccgcttg taagttagga acagtgaata ataccagaga ggtgttaaaa gaaattgttg
//
LOCUS       DANLXF010000363          894 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.364, whole genome shotgun sequence.
ACCESSION   DANLXF010000363 DANLXF010000000
VERSION     DANLXF010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.364"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            47..>894
                     /gene="msrA"
                     /locus_tag="PXD66_002556"
     CDS             47..>894
                     /gene="msrA"
                     /locus_tag="PXD66_002556"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005546455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL6320791.1"
                     /translation="MKYNQLLLCLVSAIFTTSSIANITTPNEMRSENLQDIYLAGGCF
                     WGIEAYMEKIYGVKEATSGYANGKTEQTAYRIIGQTDHAETVHVTYDANKISLEKLLQ
                     YYFKVIDPTSKNKQGNDIGRQYRTGIYYQNEQDKQIILKEIQAQQAKYKQKIQVQVEP
                     LKHYILAEEYHQDYLKKNPNGYCHIDLNQANSIIIDPKDYPKPSDATLKMKLTPLQYS
                     VTQRKNTERSFSNEYWDNTAPGIYVDITTGEPLFSSLDKYDSGCGWPSFTKPIINEVV
                     NYTEDN"
BASE COUNT          340 a          165 c          152 g          237 t
ORIGIN      
        1 ctcatcctta aaaaataaaa ttacataagt tataaaggag aactggatga aatacaatca
       61 actgttacta tgtttggtgt ctgctatctt taccacctcc tcaatagcaa acatcacaac
      121 ccctaacgag atgcgatctg aaaacttaca ggatatttac ttggcaggag gatgtttctg
      181 gggcattgaa gcctatatgg aaaaaatata tggcgtaaaa gaggctacat cgggctatgc
      241 caatggcaaa actgagcaaa ctgcttaccg aattatcggg caaaccgatc atgcagaaac
      301 agttcacgtg acctatgatg ctaataaaat ttctttagaa aagctgttgc aatattactt
      361 taaagtaatt gatccaacca gtaaaaataa acaaggtaat gatattggcc gccaatatcg
      421 gacaggtatt tattatcaga atgagcaaga taaacaaatt attttaaaag aaatacaagc
      481 tcaacaagca aaatacaaac aaaaaataca agtgcaagta gaaccattaa aacattatat
      541 tttggcagaa gagtatcatc aggattacct aaagaaaaat ccaaatggct actgccatat
      601 tgatttaaac caagccaaca gcattattat cgatccgaaa gattatccta aacctagtga
      661 tgcaacatta aaaatgaaat taacgccttt gcaatatagc gttacccaac gtaaaaatac
      721 tgagcgttca ttcagcaatg aatattggga taataccgca ccgggcattt atgttgatat
      781 aactacagga gagcctcttt tttcatctct cgataaatat gactccggct gtggctggcc
      841 aagttttacc aaaccaatta tcaatgaggt tgttaactat accgaagata atag
//
LOCUS       DANLXF010000364          887 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.365, whole genome shotgun sequence.
ACCESSION   DANLXF010000364 DANLXF010000000
VERSION     DANLXF010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.365"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            35..769
                     /locus_tag="PXD66_002557"
     CDS             35..769
                     /locus_tag="PXD66_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL6320792.1"
                     /translation="MARSSYFARLAEWTLLEHLLVKQPENISVCSFGQSFGTSGRLDA
                     EYYQPKFASLFAQLANFKTMKLGEIVNLQKSVEPGSEAYQTEGIPFVRVSDLSKFGIS
                     QTDKYLHPKDFGNVVRPKKDSILLTKDGTVGIAYRVPQDLNVITSGAIVHLELKTDEV
                     LPDYLALALNSPAVQLQAERDAGGSIIQHWKPSEILDVVIPVLPKNIQQTISDKVQQS
                     FALRVESEVLLEKAKILVEQEIENMR"
BASE COUNT          297 a          146 c          181 g          263 t
ORIGIN      
        1 ggggggggta gatcctgcta agccttatgg tttattggcg agatcttctt attttgcacg
       61 tttagcagaa tggactttat tagaacattt acttgtcaaa cagcctgaaa atatttcggt
      121 atgttcattc ggtcaatcat tcggtacaag cggtcgttta gatgcagaat attaccaacc
      181 taaatttgca agcttatttg cacaattagc caatttcaaa acgatgaaat taggtgagat
      241 tgttaattta caaaaatcgg tagaaccggg ctcggaagct tatcaaacag agggaatacc
      301 ctttgtgcgt gtatcggatt tatctaaatt cggcatcagc caaacagata aatacttaca
      361 tccgaaagat tttggcaatg ttgtaagacc taaaaaagac agtatattac tgactaaaga
      421 cggcacagtc ggcattgctt accgtgttcc gcaagatttg aatgtgatta catcaggggc
      481 aattgtgcat ctagagctta aaactgacga agttttaccc gattatttgg cgttagcatt
      541 gaattctcct gctgtacaat tacaagcgga aagagatgcg ggaggctcta ttatccaaca
      601 ttggaaaccg tctgaaattt tagatgtagt gattccggta ttacctaaaa atatccagca
      661 aaccattagc gacaaagtac aacaaagttt tgcattgaga gtagaaagtg aagttttact
      721 ggaaaaagct aaaatacttg tagaacaaga aatagaaaat atgaggtaat aatgcctaat
      781 cataatcaag atttgaaaaa agagtggttt aataaagcaa ggatagatta ccactctcct
      841 tttttaatgt tatggctcag ttgtaatagc tggtataact tccatta
//
LOCUS       DANLXF010000365          883 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.366, whole genome shotgun sequence.
ACCESSION   DANLXF010000365 DANLXF010000000
VERSION     DANLXF010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.366"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            2..628
                     /locus_tag="PXD66_002558"
     CDS             2..628
                     /locus_tag="PXD66_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDL6320793.1"
                     /translation="MTKIKFYGNLKKFGSEFSLEVKDTAEAIRALCTQISGLREALRD
                     GVYKVRIGKQYLDPSALEKGLFYCLKKGQTIHFTPVIKGAKSGGVFNFVLGAALIGAA
                     FFTGGASIAAWGAGAKMMGMLGASMLLGGVSQMLTKMPKAPTMGNETEKESSTAFSNL
                     NNLVTQGKPVPLAYGLIRTGSLVISQGVETITIKENQPASNKKTGFRK"
     gene            677..>883
                     /locus_tag="PXD66_002559"
     CDS             677..>883
                     /locus_tag="PXD66_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6320794.1"
                     /translation="MNLTKAESNLNAKITEEKKARVDADKVNADKLTDITSRVANAES
                     TITNFQSTKANKSEVASLAQSSLQA"
BASE COUNT          294 a          167 c          202 g          220 t
ORIGIN      
        1 catgactaaa attaaatttt acggcaattt aaaaaaattt ggtagtgaat ttagcttaga
       61 agttaaagat actgccgaag ctatccgagc cttatgtaca cagattagcg gattgcgtga
      121 ggcattacga gacggtgttt ataaagtccg tatcggcaaa cagtatttag acccgtcagc
      181 ccttgaaaaa gggctttttt attgtctgaa gaaagggcaa accattcatt ttacccctgt
      241 tatcaaagga gcgaaaagcg gtggcgtgtt taattttgtt ttaggtgcag ccttgatcgg
      301 agccgcattt tttactggcg gtgcttctat tgcggcttgg ggagctggag caaaaatgat
      361 gggtatgtta ggggcttcga tgttactagg aggagtttcc caaatgctca ccaaaatgcc
      421 aaaagccccc acaatgggta atgaaacaga aaaagaaagc tcaacggctt tttcaaattt
      481 aaataacttg gtgactcaag gcaaacctgt gccgttggct tatggcttaa ttcggaccgg
      541 ctcgcttgtg atatcgcagg gagttgagac gatcaccatt aaagaaaacc aaccggcaag
      601 caacaagaaa acaggcttta gaaaataagg gataattacc gattcagcac aagcacagca
      661 acttaatcag ttatctgtga acttgaccaa agcagagagc aatttaaatg ccaaaatcac
      721 ggaagaaaaa aaagctcgtg ttgatgcaga taaagtgaat gcagataaac tcacagacat
      781 aacaagccgt gttgcaaatg cggaatcaac tattaccaat ttccaatcaa ccaaagcgaa
      841 taaaagtgag gttgcgagcc ttgcacaaag ttcgttgcag gca
//
LOCUS       DANLXF010000366          867 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.367, whole genome shotgun sequence.
ACCESSION   DANLXF010000366 DANLXF010000000
VERSION     DANLXF010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.367"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..>867
                     /locus_tag="PXD66_002560"
     CDS             <1..>867
                     /locus_tag="PXD66_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator BglG"
                     /protein_id="HDL6320795.1"
                     /translation="KLNFNYQQTNEYQFVIKLLEQLREQDSRFEFNPEVIRFLTYIAI
                     ASTDLYRFKDCNSDNYPLLLDKAWEIHRFIFTQFSAYLNLSEIHDDTCRKDLLKMLIP
                     IAMKMMLSISDDVDLGLFNQTELHRNPILAHTVEKLVDDIEKNYGYSLSIREQYFIFD
                     IFSGMVNRIELERKPLRLAIIALNGRLSTQQLKRNIKRYFSDYILKIETKVLYELAIM
                     EDKPYDYYLCNEYGKNMAIPYKPIYFADSSLNENEYATSLNEVFYHAYQYDEVLPSIK
                     SIEIEAQYRLNEF"
BASE COUNT          291 a          139 c          152 g          285 t
ORIGIN      
        1 aagttaaatt ttaattatca acagacgaat gaatatcagt ttgtaattaa gttactggaa
       61 cagcttcgtg aacaagacag tcgttttgag tttaatccgg aagttattcg atttctgacc
      121 tatattgcga tagccagtac agatctctac cgctttaaag attgtaattc ggataattac
      181 ccattgttgc tagataaggc ttgggaaatt catcgtttta tttttactca gttttctgca
      241 tatttaaatt tatcggaaat tcacgatgac acttgccgta aagatttgct taaaatgctg
      301 atccctatcg caatgaaaat gatgcttagc atttcagatg atgtggattt agggctattt
      361 aaccaaacag aactacatag aaatccaatt ttggctcata cagtagaaaa attagtggat
      421 gacattgaaa aaaactacgg gtatagccta tctattcgag aacagtattt tatttttgat
      481 attttttccg gaatggttaa tcgaattgaa ttagaacgta aaccgctccg actggcgatt
      541 attgccctta atggcagatt aagcacccaa cagttaaaac ggaatattaa gcggtacttt
      601 tccgactata ttttgaaaat tgaaaccaaa gtgctatatg agcttgccat aatggaagat
      661 aaaccttatg actactatct ctgtaatgaa tatggaaaaa atatggctat tccctataag
      721 cctatttatt ttgctgacag tagtttaaat gaaaatgaat atgcaacatc actcaacgaa
      781 gtattttatc acgcttatca atatgatgag gtgttgccta gcattaaatc gattgaaatt
      841 gaagcacaat atcgcctaaa tgagttt
//
LOCUS       DANLXF010000367          867 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.368, whole genome shotgun sequence.
ACCESSION   DANLXF010000367 DANLXF010000000
VERSION     DANLXF010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.368"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..320)
                     /locus_tag="PXD66_002561"
     CDS             complement(<1..320)
                     /locus_tag="PXD66_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015483985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6320796.1"
                     /translation="MKQVMNPINTPTQRFKDGNPATGEYGTIVTAEFLNNVQDSVINT
                     QQELHSVLAEAGIEANDEQVNQVAKAIKKIAGDATRDNFNALANPDGYKHIGRCKSVA
                     ELRT"
     gene            complement(317..>867)
                     /locus_tag="PXD66_002562"
     CDS             complement(317..>867)
                     /locus_tag="PXD66_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HDL6320797.1"
                     /translation="LLPPVSYDPNGRWLALSLEVEGRELDRIDARAKTLIDAVDVSTG
                     IFIDDWERVCGLTANSSLPLETRIERVIAKLNQLGGLSIGYITQVAKELGYCIKISEP
                     QPFIAGLSRAGDMLWHKDIMWTFFVDICNPTDDYKRFRASVSMAGDALMGRYKDPILE
                     SLLEEVKPAFSRVWVRYLGDIE"
BASE COUNT          230 a          217 c          162 g          258 t
ORIGIN      
        1 atagtacgta actccgcaac cgatttgcag cgaccgatgt gtttgtaacc atcagggttg
       61 gctaatgcgt taaaattatc acgagtagca tcgcccgcaa ttttcttgat tgctttagcc
      121 acctgattaa cttgttcgtc atttgcttcg attcctgctt cagcaagtac actgtgcaat
      181 tcttgttggg tgttaatgac cgaatcttgg acgttattca aaaattcagc agtcacaatc
      241 gtgccatatt caccagtggc agggttgcca tctttaaaac gctgtgttgg ggtattaatc
      301 ggattcatca cttgtttcat tcaatatctc ctaaatatct tacccacaca cggctaaatg
      361 caggttttac ctcttcaagt agactttcta aaattgggtc tttgtatcgt cccattaacg
      421 catcgccagc cattgacaca cttgctctaa aacgcttgta atcatctgtg gggttgcaaa
      481 tatccacaaa aaatgtccac ataatgtctt tatgccataa catatcccca gctcggctta
      541 gcccagcaat aaagggttgt ggctcactaa ttttgatgca atatccaagc tctttggcca
      601 cttgagtgat atagcctatc gacaatccac ccaactgatt cagctttgca atcacacgct
      661 caattcgggt ttcaagcggt agtgagctat tcgccgttaa gccacaaacc cgctcccaat
      721 catcaataaa aatgcctgtt gatacatcaa cggcatcgat taaagtcttg gctctcgcat
      781 caatgcggtc tagctctctc ccctcaactt caagggataa cgctagccat ctgccgtttg
      841 ggtcatacga tacaggcggt aataaac
//
LOCUS       DANLXF010000368          857 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.369, whole genome shotgun sequence.
ACCESSION   DANLXF010000368 DANLXF010000000
VERSION     DANLXF010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..857
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.369"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..521
                     /locus_tag="PXD66_002563"
     CDS             <1..521
                     /locus_tag="PXD66_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AlwI family type II restriction endonuclease"
                     /protein_id="HDL6320798.1"
                     /translation="SERQGNILDFMKLSLEANLLPKTHAAGGYADIIYEYEACTSYPK
                     HSLLLEATLADGNNQRRMEMEPVSRHLGDYRIRFNNPFDYSLFVSTYLDKNVISDFRY
                     RKIIPYTRDEETITGMKIISMDTDSLKKIIENKVKYKYLYEIFDKYHEMPLETVDWHD
                     GMIKEATGEYKS"
     gene            583..>857
                     /locus_tag="PXD66_002564"
     CDS             583..>857
                     /locus_tag="PXD66_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HDL6320799.1"
                     /translation="MKNRIEHPKIFISYAWGSQEHDEKVIALATNLKGDGIDVIFDKW
                     QLKEGNDTFKFMEKSVLDESITNVLILIDPIYAKKANERAGGVGTET"
BASE COUNT          326 a          108 c          178 g          245 t
ORIGIN      
        1 tgagcgaaag acaaggaaat attttggatt tcatgaagtt atcattagaa gccaatttat
       61 tgccgaagac ccacgcagct ggaggttacg cagacattat atatgaatat gaagcgtgca
      121 cctcttatcc gaaacattca ttattgcttg aagcaaccct tgcagatgga aataatcaaa
      181 gaagaatgga aatggagcct gtttcaagac atttaggcga ttataggatt agatttaata
      241 atccgtttga ttacagccta tttgttagca catacttaga taaaaatgta atctcagatt
      301 ttagatatag aaagataatt ccatacacca gagatgaaga aaccattaca ggaatgaaga
      361 ttatttcaat ggacacagat tctcttaaga aaataattga gaataaggtt aagtacaagt
      421 atctgtatga aatatttgat aaataccatg aaatgccatt agagactgta gattggcatg
      481 atgggatgat aaaagaggct actggagagt ataagtcata gtgattataa gatagtgaga
      541 aagaatactt attttgaaag gaatacatgg gaattgatac agatgaagaa tagaattgaa
      601 catcctaaga tttttatttc atatgcttgg ggatcacagg aacatgatga gaaggttatt
      661 gctttagcta caaatcttaa aggagatggt atagatgtca tttttgataa atggcagcta
      721 aaagaaggca atgatacctt taaattcatg gaaaaaagtg ttttagatga gagtattacc
      781 aatgtactga ttcttataga tcctatttat gcaaagaaag caaatgaaag agccggtggc
      841 gtaggtacag aaacaca
//
LOCUS       DANLXF010000369          839 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.370, whole genome shotgun sequence.
ACCESSION   DANLXF010000369 DANLXF010000000
VERSION     DANLXF010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.370"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            85..>839
                     /locus_tag="PXD66_002565"
     CDS             85..>839
                     /locus_tag="PXD66_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HDL6320800.1"
                     /translation="MMKKITILGLGWLGLPLAEALQNKGWRVAGTKRTVGELAIECYP
                     LDLNALAVTSEIEQLLAADALVIALPLSQIRPENYGCGIQTLVSLAIEKGLKHLVFTS
                     STSVLPMKSGVFDENAVVDSANLLVKMENWLLSQPIHCDILRLAGLVGKKRHPVFYLA
                     GKQNLSGAGQPVNLVHLEDCIAAISLLLEQPNGQRIFHLCAEQHPSRKDYYSEMAKRF
                     GLADLHFSEENQPLVRLIKADKICQELGFRYYYP"
BASE COUNT          238 a          152 c          198 g          251 t
ORIGIN      
        1 tagaaataaa atagatagca ccacgaggtg ctttttttgt acctgaattt cccctcattc
       61 caattttaat cttgagaaac gtaaatgatg aaaaaaatta ccattttagg gcttggctgg
      121 ctcggactac ctttagctga agcattacaa aataagggct ggcgtgttgc aggtactaag
      181 cgaacggtcg gagaattagc gatagaatgt tatcctctag atttaaatgc gttagcagtg
      241 acaagcgaaa tcgagcaatt acttgctgct gatgcgttag tgattgcctt gccccttagc
      301 caaattcgcc ctgaaaatta cggttgcggt attcaaacat tagtttcgct tgcaatagaa
      361 aagggcttga aacatctggt tttcaccagc tcgacttcgg ttttgccaat gaaatcgggc
      421 gtttttgatg aaaatgcagt ggttgattct gctaacctat tagtaaaaat ggaaaattgg
      481 ttgctgagcc agccaattca ttgtgatatt ttgcgtttag ctggcttggt gggcaaaaaa
      541 cggcacccgg ttttttattt ggcgggaaaa cagaacttga gtggagcagg gcagccggtc
      601 aatttagtgc atttagagga ctgtattgca gccatttcat tgctgttgga gcagccgaac
      661 gggcagcgta tttttcattt atgtgccgaa cagcacccaa gccgtaaaga ttattatagt
      721 gaaatggcaa aacgttttgg gttagcggat ttgcattttt ctgaagaaaa tcaaccgctt
      781 gtaaggctga taaaagcaga caaaatttgc caagagcttg gttttcgtta ttactatcc
//
LOCUS       DANLXF010000370          819 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.371, whole genome shotgun sequence.
ACCESSION   DANLXF010000370 DANLXF010000000
VERSION     DANLXF010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.371"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            15..398
                     /locus_tag="PXD66_002566"
     CDS             15..398
                     /locus_tag="PXD66_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HDL6320801.1"
                     /translation="MVFTSYSIKSKKLCPTILALSVSSILSSQATYAEETTAELSEVN
                     VVAKEDVKASIEKKNKEIIQQELIQNNRDLVRYSPDVGIVNQGRHQKGFAIRGVEDNR
                     VGISIDGVSLFLIQKKIPFINAMVI"
     gene            599..>819
                     /locus_tag="PXD66_002567"
     CDS             599..>819
                     /locus_tag="PXD66_002567"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320802.1"
                     /translation="MNTFGVGYLGEKAEAILVYSNRHGHEMKSAGGYTLPEDSLQTRS
                     LRSSKQTPDDSTHNHHNYLAKFAYRFNDN"
BASE COUNT          264 a          125 c          171 g          259 t
ORIGIN      
        1 atctagggag aatcatggtg tttacctctt attcaataaa gtctaaaaaa ttgtgcccga
       61 ctatattggc tttaagcgtt agtagcattt tgtcttcaca agctacctat gcggaagaaa
      121 caacagcgga attatctgaa gtaaatgttg ttgctaaaga agatgtgaag gcttctatag
      181 aaaagaaaaa taaagagata attcagcaag agttgattca aaataatcga gatttagttc
      241 gttatagccc tgatgttggc attgttaacc aaggtagaca tcaaaaagga tttgctattc
      301 gtggagtgga agacaaccgt gtaggaataa gtattgatgg tgtctcgctt ttcctgattc
      361 agaagaaaat tccctttata aacgctatgg taatttaaat acatctcgcc aatctataga
      421 tccggaatta gctcgtacta ttgaagtatc taaaggtgca gattctttca atcaaggtag
      481 tggtaattta ggcggcggag ttaattatcg gacgcttgag ccttatgata ttgttcgtaa
      541 cggtaataaa tttggtgctt tttatcgcac aggatatggt actcgtaata atgaatggtt
      601 gaatacattc ggtgttggat atttaggtga gaaagctgag gcgatattgg tttattcaaa
      661 tcgccatggg catgaaatga aaagtgcagg aggttataca cttcctgaag attctctaca
      721 aactcgctct ttacgcagta gtaaacaaac tcctgatgat tctactcata atcatcataa
      781 ttatttagcc aaatttgctt atcgatttaa tgataatca
//
LOCUS       DANLXF010000371          799 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.372, whole genome shotgun sequence.
ACCESSION   DANLXF010000371 DANLXF010000000
VERSION     DANLXF010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.372"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(95..784)
                     /locus_tag="PXD66_002568"
     CDS             complement(95..784)
                     /locus_tag="PXD66_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL6320803.1"
                     /translation="MQNNKTTYLNWYDLCVLTLILFGEAIYTSTQQYFITTEMQGNFE
                     ENLTFSNEQNYWALLTQLALLLIAMGYLKWRNFDFSRWVIRPSWLAFFSGIGLFILLS
                     LAMDLYFETLYGVYNLVYPEPFWAVLREVNISTVLYAILNGFYEEIFFLGICLAVKPE
                     YLKWAVIYSLIIRVSFHTYQGMESALGLGIWLGLIIYLLYSRSKQKNLFPFWVAHSIA
                     DILGLSLLAYF"
BASE COUNT          278 a          173 c          130 g          218 t
ORIGIN      
        1 cctaaaagca cctttccata caaataataa tgtttttatg ctactggtac gatcagatct
       61 atcaacttat gccgcttaaa tgtagcacct ttaactaaaa ataagccaac aaactcagcc
      121 ccaaaatatc cgcaatactg tgtgccaccc aaaatggaaa taggtttttc tgctttgagc
      181 gtgagtagag caaataaata attaaaccga gccaaattcc taatcctaac gcactttcca
      241 ttccttgata ggtatgaaaa ctcacccgaa taatcagtga ataaatcacc gcccatttta
      301 aatattcagg tttaaccgcc aagcaaatac cgaggaaaaa gatttcttca taaaagccgt
      361 ttaaaatcgc atacagcacg gtagaaatat tcacttctct caatactgcc caaaatggct
      421 ccggatacac taggttgtaa acaccatata atgtttcaaa atagagatcc atcgctaacg
      481 agagcaagat aaacaacccg ataccgctaa aaaaagccag ccaagaggga cgaatcaccc
      541 aacggctgaa atcaaaattc cgccatttta gatagcccat tgcaatcagc aacaatgcaa
      601 gttgcgtcag taatgcccaa taattttgtt catttgaaaa ggttaaattt tcttcaaaat
      661 taccttgcat ttcggtggtg atgaaatatt gctgggttga ggtataaatt gcctcaccaa
      721 acaggatcag ggttagcacg cataaatcgt accaatttag gtaggtggtt ttattatttt
      781 gcattaatgt ctcctttgt
//
LOCUS       DANLXF010000372          790 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.373, whole genome shotgun sequence.
ACCESSION   DANLXF010000372 DANLXF010000000
VERSION     DANLXF010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.373"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..>790
                     /locus_tag="PXD66_002569"
     CDS             <1..>790
                     /locus_tag="PXD66_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320804.1"
                     /translation="ASIAQQNLQSIWRTDAQSAVDALKIGGANLLVDSEYLTTARWGG
                     SSRVASSQYGDRRLTQVFVTQAGTGHFGVTQGTQKATTRIRQGETYTLSLNAQGTAGF
                     TRTGLNYVYLIREDGGNFRLPTLPLTASLSQRPKVTFTAPWTSNQVRLLIGANGIFEA
                     TDWFAFHSVKLEMGNVATGWTPTAKDIDDKVSAVQSNLTAYQAAQAKADQAKATQISG
                     LTTRMGAAESNLTRTERAVTELNQTTVTTLRDLTARTKTTEGSLS"
BASE COUNT          212 a          198 c          210 g          170 t
ORIGIN      
        1 gcaagtattg cccagcagaa cttacagagc atttggcgca ccgatgcgca aagtgcggtg
       61 gatgccttga aaatcggtgg tgcaaatcta ctggttgata gtgagtattt gacgacagcc
      121 cgttggggcg gtagctcacg agtggcaagc tcgcaatatg gtgatcgtcg cttgactcaa
      181 gtgtttgtga cccaagcagg cacagggcat tttggtgtga ctcaaggcac acaaaaagcc
      241 actacgcgta ttcgtcaggg tgaaacctat accttgtcac taaatgcgca ggggacggca
      301 ggttttaccc gcacagggtt aaactatgtg taccttatcc gcgaagatgg tggcaatttt
      361 agattgccaa ctttgccgct tactgcatca ttgtcccaac gccctaaagt gacatttact
      421 gcgccgtgga cgagcaatca agttcggtta ctgattggcg caaatggcat atttgaggcg
      481 acagactggt tcgcatttca cagcgttaag ctcgaaatgg gtaatgttgc cacaggctgg
      541 acaccaaccg ccaaggatat tgacgacaaa gtatcagcgg ttcagtccaa cctcaccgct
      601 tatcaggcgg cacaagctaa agcagatcaa gccaaagcta cgcagatttc ggggctcaca
      661 acccgaatgg gggcagcgga aagtaacctc actcgcaccg aacgggcagt gacggaactt
      721 aatcagacaa cggtgacgac actgcgtgat ctaaccgcgc gcaccaaaac aacggaaggt
      781 tcgctctctc
//
LOCUS       DANLXF010000373          773 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.374, whole genome shotgun sequence.
ACCESSION   DANLXF010000373 DANLXF010000000
VERSION     DANLXF010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.374"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..715
                     /locus_tag="PXD66_002570"
     CDS             <1..715
                     /locus_tag="PXD66_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDL6320805.1"
                     /translation="QKARKFNEYNTDILYRSFDNFFAHYDTGVKHNNLSLGLNTKLSE
                     NLRGGLFFEYSNKNRNSVQIGARAKYEMDNHQLSGFLRYRGVKHKDMLARNNSVDGYL
                     NYAYRIQLDDKLSISPSVGAYVSRSSRSLIDEDLAINSRTVYASDVGLNIHYKLNDID
                     AYIRPSIGFVNGDITLSQSNDMTNTYKIKDKSNVYSVTTGLKKRFANGVALGADFKLH
                     RYGSQTTESNFGLNVSYNW"
BASE COUNT          265 a          123 c          140 g          245 t
ORIGIN      
        1 ccaaaaagcg agaaagttca atgaatataa cactgatatt ttatatcgta gctttgataa
       61 tttctttgcc cattacgaca caggtgtaaa acacaataat ctatccttag gcttgaatac
      121 aaaattaagt gagaatttac gtggtggctt attctttgaa tacagtaata aaaatcgtaa
      181 tagcgtacaa attggtgcaa gagctaaata tgaaatggat aatcaccaac tttctggctt
      241 cttacgctat cgtggcgtaa aacataaaga tatgctagca cgtaataaca gtgttgatgg
      301 ctacctaaat tatgcttatc gtattcaatt agatgataaa ttaagcatta gcccatcagt
      361 tggtgcttac gtttcacgtt cttctcgctc attaatagat gaagatcttg cgattaatag
      421 ccgcacggtc tatgcaagtg atgtaggatt gaatatccat tataagctaa atgatattga
      481 tgcttatatt cgtccaagta taggctttgt caatggtgat ataactttat ctcaatctaa
      541 tgatatgaca aacacataca aaataaagga taaaagtaac gtgtattctg tcacgacagg
      601 gttgaaaaaa cgctttgcaa atggtgtggc attaggggct gatttcaaat tacatcgtta
      661 tggttctcaa actactgaaa gtaatttcgg tctaaatgta agttataact ggtaataccg
      721 taaactgatt acgatttaaa agttagacta gcccaagact gagttctata ttc
//
LOCUS       DANLXF010000374          763 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.375, whole genome shotgun sequence.
ACCESSION   DANLXF010000374 DANLXF010000000
VERSION     DANLXF010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.375"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..714
                     /locus_tag="PXD66_002571"
     CDS             <1..714
                     /locus_tag="PXD66_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HDL6320806.1"
                     /translation="NHNKFKAKIALQGAQLLSWQPYNAEQDVLWLSEIEPFRIGSAIR
                     GGIPICYPWFGSIKEPAHGTARIQEWRLVEHTCSDENVGLVFALENKAKMEMMLGETC
                     ELYFTHLDPEPAQLALHSYFNIGDIEKVEVQGLPTRCFDKLTDQEVEVPSPRKISENV
                     DCIYPAQAVNFIQDFANQRTIKVEHINATETVLWNPWHNTVSAMTETAYQNMVCVETA
                     RINTLLQQNETLGVRVLVR"
BASE COUNT          243 a          127 c          175 g          218 t
ORIGIN      
        1 aatcacaata aattcaaagc taaaattgca ttacaaggag cacaattgct aagttggcag
       61 ccgtataatg cagagcaaga tgtgctgtgg ttaagtgaga tagaaccgtt tagaattggc
      121 tctgcgattc gtggcggtat accaatttgt tatccgtggt tcggttcaat aaaagagcct
      181 gcccacggca cagctcgcat tcaagaatgg cgtttagttg agcatacttg ttctgatgaa
      241 aatgtcggtt tggtatttgc cttagagaat aaagcgaaaa tggaaatgat gttaggcgaa
      301 acttgtgagt tatattttac ccatttagat ccagaaccag cacaactggc attgcactct
      361 tattttaata ttggtgatat tgaaaaagtg gaagtgcaag gcttgccaac tcgctgtttt
      421 gataagctaa ccgatcaaga agttgaagtg ccatcacctc gcaagataag tgaaaatgtg
      481 gattgtattt atccggcaca agcggtgaat tttattcaag attttgcaaa tcagcgcacc
      541 attaaagttg agcatattaa tgcgaccgaa accgtacttt ggaatccttg gcataatacc
      601 gttagtgcga tgacagaaac ggcgtatcaa aatatggtat gtgtggaaac cgctcggata
      661 aatactctac ttcaacaaaa tgaaacgcta ggagtgaggg ttttagtgcg atagtgttta
      721 atgagagcaa gcagctgaat tagtaatatt gtttacaaat tta
//
LOCUS       DANLXF010000375          743 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.376, whole genome shotgun sequence.
ACCESSION   DANLXF010000375 DANLXF010000000
VERSION     DANLXF010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.376"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..130
                     /locus_tag="PXD66_002572"
     CDS             <1..130
                     /locus_tag="PXD66_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CMP-N-acetylneuraminate-beta-galactosamide-
                     alpha-2, 3-sialyltransferase"
                     /protein_id="HDL6320807.1"
                     /translation="NILNKSNHIEVVALRVNVDNPAYIESYELLEKLGVQIVDIRE"
     gene            132..>743
                     /locus_tag="PXD66_002573"
     CDS             132..>743
                     /locus_tag="PXD66_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HDL6320808.1"
                     /translation="MKKYLISLEKDVKRRELFFSQPNTEDFEVFSAINTMSSDWEALN
                     NHFDLALFEQRYGRKTTKGEVGCTLSHLAVYQKIAEDSTIAEDDYCLVCEDDALFADD
                     FQKHLDALLAQNLKVDIILVGQSKIPTFNDSELELNYPSTFGFLQKNIPNSQYKYAYS
                     YLNYFAGTVAYLIKKSTACQFLAQCAGKNPYWLADDFILFEKNF"
BASE COUNT          252 a          106 c          141 g          244 t
ORIGIN      
        1 caatatttta aataaaagta accatattga agtggtagca ttaagagtga atgtagataa
       61 tcctgcttat attgagagct atgaattgct tgaaaaatta ggcgttcaga ttgttgatat
      121 tagagaataa aatgaagaaa tatttaattt cattagaaaa agatgtaaaa agacgagagc
      181 tattttttag ccagcctaat acggaagatt ttgaggtgtt cagtgccatt aatacaatga
      241 gtagtgattg ggaggcatta aataaccatt ttgatttggc tctgtttgaa cagcgatatg
      301 ggcgtaaaac cactaagggc gaagtaggtt gtactttaag ccatttagcc gtttaccaaa
      361 aaattgccga agatagcact attgcggaag atgattactg cctagtctgt gaagatgatg
      421 ccttatttgc cgatgatttc caaaaacatc ttgatgcttt attagcacaa aatttaaaag
      481 tcgatattat tttagttggg cagtcgaaaa ttccaacttt taacgatagc gaattagagc
      541 ttaattaccc atcaaccttt ggctttttgc aaaaaaatat tccgaatagc caatataaat
      601 atgcttattc ttacctgaat tattttgctg gtacggttgc atatttaatt aaaaaatcga
      661 ccgcttgtca gtttttagca caatgtgccg gaaaaaatcc atactggctt gccgatgatt
      721 ttattttatt tgaaaagaat ttc
//
LOCUS       DANLXF010000376          739 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.377, whole genome shotgun sequence.
ACCESSION   DANLXF010000376 DANLXF010000000
VERSION     DANLXF010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..739
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.377"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            6..677
                     /locus_tag="PXD66_002574"
     CDS             6..677
                     /locus_tag="PXD66_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HDL6320809.1"
                     /translation="MKKSTLAIILGGALLTGTAVAHQAGDVIFRAGAVHVKANSSSDA
                     KSKAATGIDVDLEVKNNTQLGLTATYMLADNVGIELLGATPFSHKINAKAKVLESGAE
                     VDLDRVVILKQLPPSLYAQYYFFELTAKVKPYVGAGLNYTRFYHAKSVSPAVTDLSVK
                     KHSFGPVANLGVDVKLTDNIYFNAAAWYTRIKTTAKFKAAGADHEVKVKLDPVVLFAG
                     FGVKF"
BASE COUNT          234 a          128 c          155 g          222 t
ORIGIN      
        1 tgaaaatgaa aaaatcaact ttagcaatta ttttaggtgg ggcattatta actggaactg
       61 cggtggcaca tcaagcaggt gatgtgatat tccgtgccgg tgcggtacat gttaaggcaa
      121 attcaagttc agatgcaaaa tcaaaagcag cgacaggtat tgatgtagat ttagaagtga
      181 aaaacaatac tcagttaggc ttaactgcaa cttatatgtt agccgataat gtgggtattg
      241 agttattagg tgcaacccct ttctctcata aaattaatgc caaagccaaa gttttagaat
      301 caggtgcaga agtagatttg gatagagttg ttatcttaaa acaattacca ccgagccttt
      361 atgcccaata ctatttcttt gagctaactg caaaagtaaa accttacgtt ggtgcaggtt
      421 taaattacac tcgcttctac cacgctaaat cagttagccc tgctgttact gacttaagtg
      481 taaaaaaaca ttctttcgga ccggtagcaa acctaggtgt tgatgtaaaa ttaaccgata
      541 acatctactt caacgcggca gcttggtaca cacgcattaa aacaaccgca aaatttaaag
      601 cagcaggtgc agatcacgaa gtaaaagtaa aacttgatcc ggttgtctta tttgccggtt
      661 tcggggtgaa gttctaatta tttgttggga acgagttggg ctaatcttag tgattagtcc
      721 ttttttgtta aagttgttt
//
LOCUS       DANLXF010000377          737 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.378, whole genome shotgun sequence.
ACCESSION   DANLXF010000377 DANLXF010000000
VERSION     DANLXF010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.378"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(74..379)
                     /locus_tag="PXD66_002575"
     CDS             complement(74..379)
                     /locus_tag="PXD66_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HDL6320810.1"
                     /translation="MRKPAHPGQILLDGFIEPNNIKIKELADHLGFSRETLSRVLHGK
                     TPMTANLALSLEEAGISTAKLWLNLQTKYDLWELKQQRLNPVMPFHFGGNYGETAHA"
     gene            complement(388..663)
                     /locus_tag="PXD66_002576"
     CDS             complement(388..663)
                     /locus_tag="PXD66_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011039853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL6320811.1"
                     /translation="MIKSFKHKGLKQYFEKGITKGIQLNHQRKIDGILDLIDSAESID
                     EFMPFYQCHELKGNRKGIYSMTVNGNWRITFEFVNGDAYILNYEDYH"
BASE COUNT          237 a          150 c          100 g          250 t
ORIGIN      
        1 attccttgaa taaaccgccc tcgtggcggt tttcttttcc taagaacaat aaaatcctcc
       61 gaagagggca ttattaagcg tgagcagttt ctccataatt tccaccaaaa tgaaaaggca
      121 taacaggatt taaccgctgc tgctttaact cccataaatc atatttagtt tgtaagttta
      181 gccataactt agccgtacta attcctgcct cttctaaact taacgctaaa tttgcagtca
      241 ttggtgtttt gccgtgtaat actcttgata aagtttcacg agaaaaccca agatgatctg
      301 ctaattcttt aattttgatg ttatttggtt caataaatcc atctaataaa atttgccccg
      361 gatgtgctgg tttacgcata actcccccta atgataatct tcataattca aaatataagc
      421 atcgccattt acaaattcaa atgtaatccg ccagttgcca ttaaccgtca tagaataaat
      481 gccttttcga tttcctttta actcgtggca ttggtaaaaa ggcataaatt catcaatact
      541 ttctgctgaa tcaatcaaat ctaaaatacc atcgatttta cgttgatgat taagctgaat
      601 accttttgtt attccttttt caaaatattg ttttaagcct ttatgcttga agctcttaat
      661 cataataaca cctagcaaac acaatgtgat acaaatatat cacgcacaat aaaaaagaca
      721 atctttttta agtgctt
//
LOCUS       DANLXF010000378          728 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.379, whole genome shotgun sequence.
ACCESSION   DANLXF010000378 DANLXF010000000
VERSION     DANLXF010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.379"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            12..698
                     /locus_tag="PXD66_002577"
     CDS             12..698
                     /locus_tag="PXD66_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL6320812.1"
                     /translation="MDTLASRLKIVLDQKGLTQEKFAEQIGVSQPSVFKILNGQTRNP
                     TRIYEIANVLDVDVNWLKTGKGEAPDFAKIGKNPTAYEEESSMLRLEVLDVYASAGNG
                     SFVTGDLTAYTHAVEFENAYFAQVFQRANAKGLSIINVDGDSMEPTIGNGDLLFVDTT
                     KSAYQGDGVYVFSYGENLYVKRLQFAGDELLVISDNPLYKEWRITSENEHKFQIHGKV
                     EFMQGHIRRV"
BASE COUNT          236 a          133 c          159 g          200 t
ORIGIN      
        1 aggagttcct aatggatact ttggcaagtc gtttgaaaat cgttttagac caaaaaggtt
       61 taacacagga aaaatttgca gaacaaatag gtgtttcaca gccatctgtt tttaaaatcc
      121 tgaatggaca aactcgaaat cccacaagga tttatgaaat tgcaaacgtc ctagatgtcg
      181 atgtaaattg gctcaaaacc ggcaaaggcg aagcaccaga ttttgcgaaa atcggaaaaa
      241 atccgaccgc ttatgaagag gaaagctcaa tgcttcgact tgaggtgtta gatgtatatg
      301 cctctgccgg caatggtagt tttgtcacgg gagacttaac cgcttacacg cacgcagtag
      361 aatttgaaaa tgcctatttt gcacaagtct ttcaacgtgc aaatgccaaa ggcttatcaa
      421 ttattaatgt agatggcgac tcaatggagc caactatcgg taacggtgat ttgctgttcg
      481 tagataccac taaatccgct tatcaaggcg atggtgtcta tgttttcagc tatggcgaaa
      541 acttatatgt aaaacgcttg cagttcgccg gtgatgaatt gttagtcatt tccgacaatc
      601 cgctttataa agagtggaga atcacatcag aaaatgagca taaattccaa attcacggta
      661 aagtggaatt tatgcaaggg catattagga gggtttaggt gatggaacat caaaatctaa
      721 ctcatctt
//
LOCUS       DANLXF010000379          728 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.380, whole genome shotgun sequence.
ACCESSION   DANLXF010000379 DANLXF010000000
VERSION     DANLXF010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.380"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..552
                     /locus_tag="PXD66_002578"
     CDS             <1..552
                     /locus_tag="PXD66_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL6320813.1"
                     /translation="AGQERCIADEVPFEIPQNWTWVRLGEVGDWSSGATPNRHNPEYY
                     NGTIPWLKTGDLNNGIISSIPEFITDKALQECSVRLNPVGSVLIAMYGATIGKLGILK
                     IAATTNQACCACIPFNGIYNKFLFYYLMSQKAEFQKKSEGSGQPNISKEKIINYLFPL
                     PPIHEQHRIVQKIEQLFAEIEKL"
BASE COUNT          245 a          124 c          140 g          219 t
ORIGIN      
        1 gcaggtcagg aacggtgtat cgccgatgaa gtgccatttg agattcctca aaattggact
       61 tgggtgaggt tgggggaagt aggtgattgg agttcaggtg caactccaaa ccgacacaat
      121 cctgaatact ataatggtac gatcccgtgg ctaaaaacag gagacttaaa caacggaata
      181 atttcatcta tacctgaatt tattacagat aaagcattgc aggaatgttc tgtaagatta
      241 aatcctgttg gttcagtttt gatagcaatg tatggagcga caattggtaa gcttgggata
      301 ttaaaaatag cagcaacaac aaatcaggct tgctgtgcgt gtattccttt taatggaatt
      361 tataataaat tcttatttta ttatttgatg tcgcagaaag cagaatttca gaaaaaaagt
      421 gagggaagtg ggcaaccaaa tatatcaaag gaaaaaatta tcaattattt atttccttta
      481 ccaccaatcc acgaacaaca tcgcattgtt caaaaaattg aacaattatt tgcagagatt
      541 gaaaaattgt aggagtcatc atcgctttct tgggcgtgtt ggatacgccc tacagtcact
      601 aaattattga tcttttatca tatcggtttt caaccaacct aaaaggaaac ctatatgatc
      661 catctatttg aaaagcacct taatcaatct aatcttgctg aaaataccca atcagcttat
      721 ctttttgc
//
LOCUS       DANLXF010000380          704 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.381, whole genome shotgun sequence.
ACCESSION   DANLXF010000380 DANLXF010000000
VERSION     DANLXF010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.381"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..>704
                     /locus_tag="PXD66_002579"
     CDS             <1..>704
                     /locus_tag="PXD66_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320814.1"
                     /translation="KKLYSRVGYDKGVWEALSVLLPERFDFEVFEVIDYSKEWSWFEI
                     IVENLFWRQPEKINMQKLHQFFKNSPYFNQATWINILYQLATEINHPLNIKYLHNKLY
                     SFSLADRDEIWTAFIALESDQCPAIERLLTWCQKSSGQENIEPEALLLGAIAVSWLFT
                     STDKRSRNGYINALAHLLWNRLEIALALFKEFIEVNDPYVMEGILSAIHGAVAYSENS
                     SGLKALALEIDNQIFN"
BASE COUNT          235 a           96 c          134 g          239 t
ORIGIN      
        1 aaaaaagcta tatagtagag ttggctatga taagggcgta tgggaagcat taagtgtatt
       61 gttgccagaa cgatttgatt ttgaagtatt tgaagtgatt gattattcaa aggaatggtc
      121 ttggtttgag attattgtag aaaatctctt ttggaggcag cctgaaaaaa tcaatatgca
      181 aaaactacat caatttttca aaaatagtcc atattttaat caagcaactt ggattaatat
      241 tttatatcaa ctggctaccg aaattaacca cccattaaat ataaaatatt tacataacaa
      301 actttattct ttttctttgg cagatagaga tgagatttgg acagctttta ttgctttgga
      361 gtctgatcaa tgcccagcaa ttgaacgatt actaacttgg tgtcagaaat caagtggtca
      421 agaaaatatt gaacccgaag cactattatt gggtgcaatt gctgttagtt ggctatttac
      481 ttctaccgat aaacgaagta gaaatggcta tattaatgca ctggctcatt tattatggaa
      541 tagattggaa attgcattgg cgttattcaa ggaatttata gaggttaatg acccttatgt
      601 tatggaaggt attttatctg ccattcacgg agctgttgct tattctgaaa attcatctgg
      661 cttaaaagca cttgcattag agatagataa tcaaatattt aatc
//
LOCUS       DANLXF010000381          701 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.382, whole genome shotgun sequence.
ACCESSION   DANLXF010000381 DANLXF010000000
VERSION     DANLXF010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.382"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            6..>701
                     /locus_tag="PXD66_002580"
     CDS             6..>701
                     /locus_tag="PXD66_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL6320815.1"
                     /translation="MAIYAIWNNKGGVGKSYLTFQIACEYAKQYPAKKVLVVDLCPQA
                     NSSSMLLGGIEKGEQELAKIHSQVNKNTISGYISERLVSPYRSINSATKYVTQVSTIN
                     EYVPKNVYLVVGDEELEIQSSGILHASQAVHVPDSWRLVHLWVRDLVSDIQNAWDNDD
                     NVVFIDCNPSFTIYTELALAASDRLIIPFSADGSSKRAVKSVLSLVYGVKRHSGDVSS
                     HFYTESQKSKLALP"
BASE COUNT          217 a          109 c          136 g          239 t
ORIGIN      
        1 gcattatggc tatttatgca atatggaata ataaaggtgg ggttggtaaa agttatttaa
       61 cttttcaaat cgcttgtgag tatgcaaaac aatacccagc aaaaaaagtt ctcgttgttg
      121 atttatgtcc tcaagcaaat tcatcatcta tgctattagg cggcattgaa aaaggtgagc
      181 aagagttagc aaaaattcat tctcaagtaa acaagaatac tatttcagga tatatttcag
      241 aaagattagt aagtccttac cgttctatta atagtgcaac gaaatatgta actcaagtta
      301 gcactataaa tgaatatgta ccaaaaaatg tttatcttgt tgttggtgat gaggaattag
      361 aaatccaatc atctggtatt ttacatgctt cccaagctgt tcatgttcct gattcttggc
      421 ggctagtgca tctttgggtg cgtgatttag tttctgatat acagaacgct tgggataatg
      481 atgataacgt tgtatttatt gattgcaatc caagtttcac tatttatact gaattagcgt
      541 tggctgcttc agatagattg atcatccctt tttccgctga tggttcatca aaacgagctg
      601 ttaagtctgt attatctcta gtatatgggg ttaaaaggca ctctggggat gtcagctctc
      661 atttttatac agaatctcaa aagagtaagt tagcattacc t
//
LOCUS       DANLXF010000382          688 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.383, whole genome shotgun sequence.
ACCESSION   DANLXF010000382 DANLXF010000000
VERSION     DANLXF010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.383"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..446)
                     /locus_tag="PXD66_002581"
     CDS             complement(<1..446)
                     /locus_tag="PXD66_002581"
                     /inference="COORDINATES: protein motif:HMM:NF016343.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HDL6320816.1"
                     /translation="MNEDKFFSVDVSNDIHILNLHETLEQAKQSCLNGATEAYEFADD
                     MDDHESYEAYDLPYAVYGVVLGRAKSDIRPLTDEERESELFGEAEQVIEPPTLVENNG
                     WISVKDRLPEIRKEVLITNNFIMIAQFNGESWFKPGGFLGIQPVYGV"
BASE COUNT          201 a          144 c          114 g          229 t
ORIGIN      
        1 acaccataga caggctgaat acctaagaat ccacccggct tgaaccagct ctcgccattg
       61 aattgagcaa tcataataaa attgtttgtt atcaagactt ctttgcggat ttctggcaat
      121 ctgtctttga ctgaaatcca gccgttgttt tctacaagtg ttggtggttc aataacttgt
      181 tcagcttccc caaataactc tgattctctt tcttcatcgg ttagtgggcg gatatctgat
      241 tttgctctac ctaaaacaac accataaact gcatagggta aatcataggc ttcatagctt
      301 tcgtgatcat ccatgtcatc cgcaaactca taggcttcag tagcaccatt taaacagctt
      361 tgttttgctt gctctaaggt ttcgtgcaga tttaggatat gtatatcatt tgatacatcc
      421 accgaaaaaa atttgtcttc attcattttc tttctccaaa ataccaatag ttgagagaat
      481 aataagcata ctatctcatg ctattcttaa gtgcatctaa ccacgctcca gcatcttcgg
      541 ctgaagcgaa atataaccct tgatcaaata gtttttctat ataagaatgt gtagggaaat
      601 ttgattttat cacattgaaa ttatcgccaa taaaccacat tccatctctt ggttctttta
      661 atggacaagg aagagtgaga gttactgt
//
LOCUS       DANLXF010000383          686 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.384, whole genome shotgun sequence.
ACCESSION   DANLXF010000383 DANLXF010000000
VERSION     DANLXF010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.384"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..>686
                     /locus_tag="PXD66_002582"
     CDS             <1..>686
                     /locus_tag="PXD66_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HDL6320817.1"
                     /translation="TLNNKSDSILFVESTWQGYKNRWKYKIASYPDHPKRTNQKLLRL
                     VEKAKDKGIPTVFWNKEDSIHFDRFIDSAKHFDHIFTVDQNAIARYREVVPDTTTVNV
                     GMFPVQPRIHNFQGFNFKHLAANFVGSYSHHIHNKRRERQDMLFGAALKAGLPVTIFD
                     RNSDRKSENYRYPKNSFNLNVQPAVSYTETANIYRDYLVSLNINTVEDSPTMFSRRVV
                     EILACGGILV"
BASE COUNT          226 a          116 c          125 g          219 t
ORIGIN      
        1 tgacgttgaa taacaaatca gattcaatat tatttgtaga atctacttgg caaggctaca
       61 aaaaccgttg gaaatacaaa atagcatctt atcctgacca ccccaaacga accaatcaaa
      121 aattgttgcg tttggtggaa aaagcaaaag ataaaggtat tccaacggta ttttggaaca
      181 aggaagattc catacatttc gaccgattta ttgattcagc gaagcatttt gatcatatat
      241 ttactgtaga tcaaaatgct attgctcgtt atagagaggt tgtacctgat acaaccactg
      301 tcaatgtagg tatgtttcct gttcagccaa ggattcataa ttttcaagga tttaatttta
      361 agcaccttgc cgcaaatttt gtagggagtt acagtcatca tatccacaat aagcgtagag
      421 aaagacagga tatgttgttt ggtgcagcac ttaaagcagg tcttccggta accatctttg
      481 atagaaactc tgatagaaaa tcggaaaatt atcgttaccc aaaaaatagc tttaatttaa
      541 atgttcaacc tgctgttagt tatactgaaa ctgctaatat ttatcgtgat tatcttgttt
      601 ctttgaatat taatactgtc gaagattccc ctacgatgtt ttctcgcaga gtcgttgaaa
      661 tattagcttg tggaggaata ttagtc
//
LOCUS       DANLXF010000384          657 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.385, whole genome shotgun sequence.
ACCESSION   DANLXF010000384 DANLXF010000000
VERSION     DANLXF010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.385"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(17..655)
                     /locus_tag="PXD66_002583"
     CDS             complement(17..655)
                     /locus_tag="PXD66_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL6320818.1"
                     /translation="MELKHTIEQRKTIKAFNSTIKISRTELEEMVALSQLAPSKANLQ
                     PWRFVIIDVEILKAALSKNVAFNGPPCETASAIVVILADLQYEKLLGAIVDQSVETGC
                     LHKEFRERKFDFLLNLHNQATQAEIRDQVLIDTSLAAMQLMLIAKDKGYDTHAIGVFE
                     RPAVLKLLNIDAERYLPVMLLAIGKAANPALPSCRLPLEYTVSWNDGQGFKI"
BASE COUNT          201 a          135 c          107 g          214 t
ORIGIN      
        1 tttaaccgct tgcaccttat attttaaaac cttgtccatc gttccaagaa accgtatatt
       61 ctagaggtaa acggcaactt ggcaacgctg ggtttgctgc tttaccgata gctagtagca
      121 taacaggcaa ataacgttct gcatctatat tcaataattt taatacagca gggcgctcaa
      181 acacgcctat cgcatgagta tcatagccct tatctttggc aattagcatt aattgcattg
      241 ccgccaaact agtatcaatt aatacctgat ctctaatttc tgcttgagtt gcttggttat
      301 gaagatttag taaaaaatca aatttacgtt cacgaaactc tttatgcaaa cacccagttt
      361 ctaccgattg atcaacaatt gcgcctaata atttttcata ttgcaaatct gccaatatca
      421 ccacaatagc agacgctgtt tcacaaggtg ggccattaaa ggcaacattt tttgataatg
      481 ctgctttaag aatttcaaca tcaataatga caaaacgcca aggttgcaga tttgcttttg
      541 agggagcaag ctgggataaa gcgaccattt cttctaattc agttctacta atcttaattg
      601 ttgaattaaa agctttgata gttttacgct gttcaatcgt atgtttcaat tccatct
//
LOCUS       DANLXF010000385          653 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.386, whole genome shotgun sequence.
ACCESSION   DANLXF010000385 DANLXF010000000
VERSION     DANLXF010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.386"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            60..>653
                     /locus_tag="PXD66_002584"
     CDS             60..>653
                     /locus_tag="PXD66_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDL6320819.1"
                     /translation="MFTEGKIDFDKFQQLFSDNFSTEPDRYMLNWAGKSTAYQTLQTP
                     TSKTLTPCEEESVDFENTQNIFIEGENLDVLKALQKSYFNSVKMIYIDPPYNTGNDFI
                     YNDSFADTKADYAEKVGDVDEHGKLKRAFVRNSKENGHYHSNWLNMMLPRLHLAKNLL
                     RDDGVIFISIDDNEQAQLKLLCDEVFGEENFVAILSVE"
BASE COUNT          213 a          128 c          126 g          186 t
ORIGIN      
        1 ttgccaactt ttcgcttaaa gatcaacaac tttctcaatt aaaatccctt ttacctgaaa
       61 ttttcaccga aggtaaaatc gacttcgaca aattccaaca acttttttcg gataattttt
      121 ccaccgaacc tgaccgctat atgctcaact gggcaggaaa atcaacagcc tatcaaacct
      181 tacaaacccc aaccagcaaa acgctcactc cttgcgagga agagagtgtg gattttgaaa
      241 atacgcagaa tatctttatt gagggtgaaa atttagatgt attaaaagcc ctgcaaaaat
      301 cctacttcaa ttcggtcaaa atgatttata tcgatccgcc atacaataca ggcaacgatt
      361 ttatttataa cgacagtttt gccgatacca aagcagatta tgcggagaaa gtgggcgacg
      421 ttgatgaaca cggcaaattg aaacgagcct ttgttcgtaa cagcaaagaa aatgggcatt
      481 atcacagtaa ctggctgaat atgatgttgc ctcgtttgca tctcgcaaaa aatttactgc
      541 gtgatgacgg ggtgattttt atttcaattg atgataacga acaggcacag ctgaaattgc
      601 tttgtgatga agtgtttggg gaggagaatt ttgttgccat attatcagtt gaa
//
LOCUS       DANLXF010000386          650 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.387, whole genome shotgun sequence.
ACCESSION   DANLXF010000386 DANLXF010000000
VERSION     DANLXF010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.387"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..450)
                     /locus_tag="PXD66_002585"
     CDS             complement(<1..450)
                     /locus_tag="PXD66_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320820.1"
                     /translation="MAVSAYIGIPGSGKSYEVVKSVILPAVASNRRVVSNIYGLNKEK
                     IHQYLLRKNKKLMIEELGEVIYVTNEQCLESDFLPSMDEQDSFCKAGDLIIIDEVWRI
                     WGNDKDIPKNHRSFIAEHRHFADPITGVTCDLVVINQDISQIPRFIKE"
     gene            complement(460..>650)
                     /locus_tag="PXD66_002586"
     CDS             complement(460..>650)
                     /locus_tag="PXD66_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HDL6320821.1"
                     /translation="ELLPVVIEMLVSRKLIPDLNALFGQLPESMWYFLNIFQIPMAIS
                     LMISAMISRFVRRMPFIG"
BASE COUNT          224 a          130 c           86 g          210 t
ORIGIN      
        1 ttctttaata aatcttggaa tttgagaaat atcttgattt attacgacta aatcacaagt
       61 tacacctgtt ataggatctg caaaatgtcg atgttcagca ataaatgaac gatgattttt
      121 tggaatatcc ttgtcgtttc cccaaatacg ccaaacttca tcaataatta tcaagtcccc
      181 agctttgcaa aatgaatctt gttcgtccat tgatggtaaa aaatccgatt ccaagcattg
      241 ttcattcgtt acatatatga cttcaccaag ctcttcaatc attaattttt tatttttacg
      301 cagtaaatat tgatgaattt tctctttatt taatccataa atgttggaaa caactcttcg
      361 attcgaagca acagcaggta aaattactga cttaaccact tcatacgatt tgccgcttcc
      421 aggaatccct atatacgctg aaacagccat agcactcctc tatccaataa atggcatacg
      481 tctaacaaaa cgtgaaatca ttgctgaaat cattaaagaa atagccattg gtatctgaaa
      541 aatatttaaa aaataccaca tactttcagg caattgccca aataacgcat ttaaatcagg
      601 tatcaatttt ctagacacaa gcatctctat tactacagga agtagttcag
//
LOCUS       DANLXF010000387          648 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.388, whole genome shotgun sequence.
ACCESSION   DANLXF010000387 DANLXF010000000
VERSION     DANLXF010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.388"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..>648
                     /locus_tag="PXD66_002587"
     CDS             <1..>648
                     /locus_tag="PXD66_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL6320822.1"
                     /translation="ELLSLEGFKMKVVHNGLEGLNELESNQYDLVLLDVMMPVLSGVK
                     TLKRIRQKYSLPVLMLSARDDQIDRILGLELGADDYVPKPFNNRELVARMKAILRRTT
                     SRPASTDHSEQNYTENISKSLQFAGVELHSGRQQAYYQGCDLDLTGTEFALLQMLMRN
                     SGEILSRELLSLKILGKNLTPYDRAIDMHISNLRKKLPEREDGLPWFKTLRGRGYL"
BASE COUNT          224 a           94 c          130 g          200 t
ORIGIN      
        1 gaattacttt cattagaagg atttaaaatg aaagttgtgc ataatggttt agaaggctta
       61 aatgagttag agtctaatca atatgattta gtattactag atgtgatgat gccggtatta
      121 agtggcgtga aaacattaaa acgtatacga caaaaatact cattacccgt tttaatgtta
      181 agtgcaagag atgatcaaat tgatagaatc ttaggtttag aattaggggc tgacgattat
      241 gtaccgaaac cttttaataa tcgtgaacta gtcgctagaa tgaaagcgat acttcgaaga
      301 acaacctctc gcccagcttc aacagaccat tctgaacaga attatactga aaacatctct
      361 aaatcattac aatttgccgg agttgaatta cattcaggac gtcagcaggc ttattatcaa
      421 ggatgtgatc ttgatttaac ggggactgaa tttgcattat tacaaatgtt aatgcgaaac
      481 tcaggggaga ttttgtccag agaattattg agtttaaaaa ttttagggaa aaatttaacg
      541 ccttatgatc gtgcaattga tatgcatatt tctaatcttc gtaaaaaatt acccgaacgg
      601 gaagatggat taccttggtt taaaacctta agaggtcggg gttactta
//
LOCUS       DANLXF010000388          644 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.389, whole genome shotgun sequence.
ACCESSION   DANLXF010000388 DANLXF010000000
VERSION     DANLXF010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.389"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..>644
                     /locus_tag="PXD66_002588"
     CDS             <1..>644
                     /locus_tag="PXD66_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL6320823.1"
                     /translation="EIEQTRAAYDRLKRSGVASQNELRRASEATKQRVRELNAELGKT
                     SFGDKAKGFGRGLMSAGVGIAAGAAMARQPAKNQMEFDRQLAMASNTAFSDRDVKGRI
                     EGKKELLAAVQEAVKVGGGTKEDALLTLDTLIASGTMKAETAMSLLPTLQKGAVATGA
                     SSEDMAKIAISSMQQFGIKEEDIGRALDMAVAAGQAGNFELADMARWLPQQMAAG"
BASE COUNT          184 a          129 c          190 g          141 t
ORIGIN      
        1 gaaattgaac aaactcgtgc agcctatgac cgcttaaaac gctcaggtgt tgcctctcaa
       61 aatgagctac gccgtgcctc agaagccact aagcaacggg tgcgagagct gaatgcagaa
      121 ttgggtaaga caagctttgg ggataaggct aaaggatttg ggcgtggttt gatgagtgcc
      181 ggtgtcggta ttgcagcagg tgctgcaatg gctcgtcagc cagccaagaa ccaaatggag
      241 tttgaccgac agttagcgat ggcatcaaac actgcctttt ccgaccgtga tgttaaaggg
      301 cgtattgagg gcaagaaaga gttattagca gcggtgcaag aggcggttaa ggttgggggt
      361 ggaacaaaag aagatgctct tttaactctt gatacgctaa ttgcctcagg aacgatgaag
      421 gctgaaactg caatgagcct attaccaacc ttgcagaaag gtgctgtagc aacaggtgca
      481 agctccgagg atatggcaaa aattgcgatc tcctcaatgc aacaatttgg gattaaagaa
      541 gaggatattg ggcgggcatt ggatatggca gttgcagccg ggcaagcagg taactttgag
      601 cttgcagata tggctcgctg gctaccacaa caaatggccg cagg
//
LOCUS       DANLXF010000389          643 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.390, whole genome shotgun sequence.
ACCESSION   DANLXF010000389 DANLXF010000000
VERSION     DANLXF010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.390"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..493
                     /locus_tag="PXD66_002589"
     CDS             <1..493
                     /locus_tag="PXD66_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6320824.1"
                     /translation="ARPLNTLEVKGLTVVDDSQKPLFSEVNQALYHGLSPIEVVASRV
                     QITRAITTYTKNVTNTDDPSYLDLTTIRTLDYVRKAIQTRQRLRFPRAKNSHRIVAKV
                     RSEILDVLYQLEGEEVIENVEAWKNRLLVVRNQDPTYLDLEIPADVVNGLHVIRNKIT
                     LIL"
     gene            504..>643
                     /locus_tag="PXD66_002590"
     CDS             504..>643
                     /locus_tag="PXD66_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6320825.1"
                     /translation="MSIVYEGSCVLEVDGVEIEITKLDVKTVTGRKLVKTMNSSGRAR
                     GYS"
BASE COUNT          182 a          127 c          160 g          174 t
ORIGIN      
        1 ggctcgcccg ttaaataccc ttgaagttaa aggcttaacg gtcgtagatg actcgcagaa
       61 accgttattt agcgaagtaa accaagcgtt atatcacggt ttaagcccga ttgaggtggt
      121 ggcaagccgt gtgcagatta cccgtgcgat tactacatac accaaaaacg tgacgaatac
      181 tgatgaccca agctatttgg atttaaccac cattcgtacg cttgactatg tgcgtaaggc
      241 aatccaaacc cgtcagcgtt tgcgtttccc tcgtgcgaaa aactcccacc gtattgtggc
      301 aaaagtacgc tcggagattt tagatgtgct ttatcagctt gagggcgaag aggtaatcga
      361 aaatgtggaa gcgtggaaaa atcgcttatt agtggtgcgt aatcaagacc cgacttactt
      421 ggatttagaa atccctgcgg atgtggttaa tggcttgcac gttattcgta acaaaattac
      481 gttaatttta tagggggtgc tagatgtcaa ttgtttatga aggctcttgc gtgcttgagg
      541 tagatggcgt tgagattgaa attaccaaac ttgatgtaaa aaccgtaacc ggtcgcaagc
      601 tggttaaaac gatgaacagt tccggacgtg caagaggtta ttc
//
LOCUS       DANLXF010000390          633 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.391, whole genome shotgun sequence.
ACCESSION   DANLXF010000390 DANLXF010000000
VERSION     DANLXF010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.391"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            61..>633
                     /locus_tag="PXD66_002591"
     CDS             61..>633
                     /locus_tag="PXD66_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320826.1"
                     /translation="MREPLLDEDELKKWLQTIFTDNLVIIVGSGLSCAEGLPGMGALA
                     DRLKERMPECLDDIDKVTWNTISDCLDSEGLEGALLKHQANETIEAAIIKITAEYVLS
                     EEQKAINKCIAENQKLKFSYLLPHISASNPKIARVITTNYDRLIEFAAEYENWGIDSM
                     MVGRYWGKHNPDLSRKLQIRDIRVKGKCPKL"
BASE COUNT          217 a           84 c          146 g          186 t
ORIGIN      
        1 atgacgtctc gtagtaaaca gctagtttgt aagaatagat atttggaagg gaaacgtaaa
       61 atgagagaac cattattaga tgaagatgaa ttaaaaaaat ggttgcaaac tatttttact
      121 gacaatttgg tcattattgt cggttcgggt ttgtcctgtg ccgaaggttt gccgggaatg
      181 ggagcattag ctgatagatt aaaagaacga atgcctgaat gtcttgatga tatagataag
      241 gtaacatgga ataccatttc tgattgtctg gattctgaag gcttggaagg tgcattgttg
      301 aagcatcaag caaatgagac gatagaagcc gctattatta aaataacagc cgagtatgta
      361 ctctccgaag agcaaaaagc gataaacaaa tgtattgctg agaatcagaa gctcaaattt
      421 tcttatttgc ttccccatat ttcagcttca aatcctaaaa ttgcccgtgt aattactact
      481 aattatgata gattgattga gtttgcggca gagtatgaaa attggggaat agattcaatg
      541 atggtcggtc gttattgggg aaaacataat cctgatttga gtaggaaatt gcagataaga
      601 gacattagag taaaaggtaa atgtccaaaa ttg
//
LOCUS       DANLXF010000391          624 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.392, whole genome shotgun sequence.
ACCESSION   DANLXF010000391 DANLXF010000000
VERSION     DANLXF010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.392"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..617)
                     /locus_tag="PXD66_002592"
     CDS             complement(<1..617)
                     /locus_tag="PXD66_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL6320827.1"
                     /translation="MAEILFRSCLCCNAKRIFYLLTFYKGFETSQIATAFSLLVFTSQ
                     AFSLFAGSWGDSFGRYPIMMLGCLLDALAYILLLTTKNYPLLLLATFFFGLGSTLFST
                     NARAFLLANIQDTGDSYQLKTKAQGKFLKVSSLSAMVAPLLTIPFIFYRKAEWLIWIS
                     CCIEIIMLFVMFKTAPKTHECANGQCERFKFGALKGILNEQFIFV"
BASE COUNT          217 a          109 c          126 g          172 t
ORIGIN      
        1 tgtacaaaaa taaattgttc atttagtatt ccttttaatg caccaaattt aaagcgttcg
       61 cattgcccat ttgcacattc atgtgttttg ggtgcggttt taaacataac gaatagcata
      121 ataatttcaa tgcagcagct aatccaaata agccattctg ctttacggta gaaaataaat
      181 ggaatagtaa gtaatggtgc taccatcgct gacaggcttg ataccttcag aaatttacct
      241 tgagccttag ttttgagttg gtaactatct ccggtatctt gaatattggc taataagaat
      301 gctctcgcat tggtactaaa taaggtgcta ccgagcccga agaaaaaggt agcaagtaat
      361 agcagagggt aattttttgt tgtcagtaat aaaatgtaag ctagggcatc gagcaaacag
      421 ccaagcatca tgatagggta acgtccaaaa ctatcacccc aagaacctgc aaaaagggaa
      481 aatgcttggc tggtaaagac caacaaagaa aatgcagttg caatttggct ggtttcaaag
      541 cctttgtaga atgtaagtag ataaaaaata cgctttgcat tgcagcataa gcaagaccgg
      601 aaaagaattt ccgccataaa atga
//
LOCUS       DANLXF010000392          623 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.393, whole genome shotgun sequence.
ACCESSION   DANLXF010000392 DANLXF010000000
VERSION     DANLXF010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.393"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..416)
                     /locus_tag="PXD66_002593"
     CDS             complement(<1..416)
                     /locus_tag="PXD66_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDL6320828.1"
                     /translation="MQQLIKNIEQWAEDRNLINGSTPQKQMLKLMEEFGELCGGIARN
                     NPEMIKDAIGDLIVVCIIKAKQEKQPIYPVQFENHRLNGYRFDISVAVHCLYDIERIK
                     DRGKYSRFFGILHHLALINRFTLNDCLAHAYDQIKDR"
     gene            complement(400..603)
                     /locus_tag="PXD66_002594"
     CDS             complement(400..603)
                     /locus_tag="PXD66_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320829.1"
                     /translation="MTDTERLDFIAQRKLDVRYSDLVDEFIIASYTKPHEQYFHWARN
                     ADLRTAIDEAMKEMRGLNNATTN"
BASE COUNT          162 a          122 c          120 g          219 t
ORIGIN      
        1 cgatctttga tttggtcgta ggcgtgagcg aggcagtcat taagcgtaaa tctatttatt
       61 aaggctaaat ggtgtaatat gccaaaaaaa cggctatatt tacctctgtc tttaatgcgt
      121 tcaatatcat atagacaatg aacagcgacc gaaatatcaa atctgtaacc atttaatcta
      181 tgattttcaa attgtacagg gtaaataggt tgtttttctt gttttgcttt aataatacag
      241 acaacaatca aatccccaat cgcatcttta atcatttcag gattattgcg ggcaatgcct
      301 ccgcagagtt cgccaaactc ttccatcagt ttgagcattt gcttttgtgg ggttgagccg
      361 ttaattaaat tgcggtcttc cgcccattgt tcgatgtttt taattagttg ttgcattgtt
      421 tagtcctctc atttctttca ttgcttcatc aatcgccgtg cgtaaatcgg catttcttgc
      481 ccaatgaaaa tactgctcgt gcggtttggt atagctagca ataataaatt cgtccaccaa
      541 atcagaatat cggacatcta atttgcgttg ggcgataaag tctaagcgtt cggtgtcggt
      601 catttttcct cctttggtgg ttc
//
LOCUS       DANLXF010000393          619 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.394, whole genome shotgun sequence.
ACCESSION   DANLXF010000393 DANLXF010000000
VERSION     DANLXF010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.394"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..>619)
                     /locus_tag="PXD66_002595"
     CDS             complement(<1..>619)
                     /locus_tag="PXD66_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL6320830.1"
                     /translation="LIDLETQKQVDKIIKQSNDFVQKSTQSYQEAETLLLENLGLRAF
                     QADSNPVNVKSLKESFLQTGRLDAEYYQTKYEQYWNLIQSQDYVFIRDEYLHITQKPD
                     WTKPMYQYIEIGDVNIGDGSYQTNWIETQELPANAKTQAQTGDILISTVRPYRGAVTI
                     IGENDQDLVVSGAFTVLRRKENSVFNNEVLKVLLRSELYKDWLLQF"
BASE COUNT          192 a          108 c           88 g          231 t
ORIGIN      
        1 aaattgtagt aaccaatctt tatatagttc tgaccgtaat agcaccttta aaacttcatt
       61 attaaaaaca gagttttcct ttctgcgtaa tactgtaaac gcaccactaa caactaaatc
      121 ttgatcattt tcaccgataa ttgttactgc accacgataa gggcgaacgg ttgaaattaa
      181 aatatcacct gtttgggctt gagttttagc attagcaggt aattcttgtg tctctatcca
      241 attcgtttga tatgaaccat caccaatatt aacatcacca atttcaatat attgatacat
      301 cggttttgtc caatcaggtt tttgggtgat atgtaaatat tcatcacgaa taaaaacata
      361 atcttgcgat tgaattaaat tccagtattg ctcatattta gtttgataat actctgcatc
      421 taatcgtcct gtttgtaaga aactctcttt taaggattta acatttacag gattgctatc
      481 agcttgaaaa gctctcaaac cgagattttc taataataaa gtttcagctt cttggtaact
      541 ttgtgttgat ttttgtacaa aatcattact ttgcttaatg attttatcta cctgcttttg
      601 tgtttctaga tcaattaag
//
LOCUS       DANLXF010000394          616 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.395, whole genome shotgun sequence.
ACCESSION   DANLXF010000394 DANLXF010000000
VERSION     DANLXF010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.395"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..113)
                     /locus_tag="PXD66_002596"
     CDS             complement(<1..113)
                     /locus_tag="PXD66_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL6320831.1"
                     /translation="MFSIIVPSYNRNQEINALLESLKQQTAYNFEVIIVDD"
     gene            complement(106..>616)
                     /locus_tag="PXD66_002597"
     CDS             complement(106..>616)
                     /locus_tag="PXD66_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320832.1"
                     /translation="IAFTALILFLAIVFPENRYVEHALEPVINLLSGTGKVSSSTDNL
                     VKNHLFLPTLNQFISGDGLYYTETGGYYGGSDSGYIRQVLYGGVSYMLACFAFTYYFV
                     RKVAVNWFEGSWIFTLSTIGILAILNIKADTFAFPGLMMLFLMFLSLFGTSGRNVTFF
                     CKNKEAEYV"
BASE COUNT          222 a          129 c          103 g          162 t
ORIGIN      
        1 cagtcatcaa caatgatcac ttcaaaatta tatgcagttt gttgctttaa actctcaagc
       61 aacgcattga tttcttgatt acggttataa gaaggcacaa taatactaaa catattcagc
      121 ctctttgttt ttgcaaaaaa atgttacatt tcgaccgctt gttccaaaca gcgacaagaa
      181 cattagaaac agcatcatca gccccgggaa agcaaaggta tctgctttaa tatttaaaat
      241 agctaaaatg ccgatggtgg ataaggtaaa aatccaactg ccttcaaacc aattcaccgc
      301 cactttgcgt acaaaatagt aagtgaaggc aaaacacgcc aacatataac ttactccacc
      361 atataatact tggcgaatat agccggaatc cgatccacca taatagccac cagtttcggt
      421 gtaatataaa ccatcaccgg aaataaactg atttaaagtc ggtaaaaaca gatggttttt
      481 cactaaatta tcggttgaag agctgacttt tccggtgccg gacaataaat taattaccgg
      541 ttctaaggcg tgttcaacat aacggttttc cggaaaaaca atcgccaaaa atagaattaa
      601 cgcagtgaag gcaatc
//
LOCUS       DANLXF010000395          613 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.396, whole genome shotgun sequence.
ACCESSION   DANLXF010000395 DANLXF010000000
VERSION     DANLXF010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.396"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(25..>613)
                     /locus_tag="PXD66_002598"
     CDS             complement(25..>613)
                     /locus_tag="PXD66_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320833.1"
                     /translation="GFFNFFPEFITILGTGAFNGFLDFPSLVGKVFPEIIDTINEGLD
                     LDIAIENFEVLSQEEMTECMGLYEQALIKKAEEGEIGLLNVDEINSFYEDNSELILLA
                     KQPMVEEIIPKPDSKASSAILKFFDSNSNILIAKLKESGLQLSAVALQNDQNIARVAN
                     VVYNLLPGMVRIFVSYDTVENFLLNNRQWLINKLV"
BASE COUNT          202 a          126 c           80 g          205 t
ORIGIN      
        1 tatccatagt tcttcccctc caaattaaac taatttatta atcaaccatt ggcggttatt
       61 taataagaaa ttttctacgg tatcgtaact tacgaaaatt cttaccatgc ccggcaataa
      121 gttataaacg acatttgcta ctcgtgcgat attttggtcg ttttgtaatg ccacagcact
      181 taattgcaaa ccactttctt ttaatttagc aattaagata tttgagtttg aatcaaagaa
      241 ttttagaatt gcagaggagg ctttgctatc tggtttaggg attatctctt caaccatagg
      301 ttgtttagca agtaagatta gctcactatt atcttcataa aagctattta tttcatcaac
      361 atttaataaa ccaatttcgc cttcttcagc tttcttaatg agagcctgct catataaacc
      421 catacattca gtcatttctt cctgactcaa cacttcaaag ttctcaatcg caatatctaa
      481 atctaaaccc tcatttatcg tatcaataat ttctggaaaa acctttccta ccaaactagg
      541 gaagtctaaa aatccattaa acgcaccagt cccgagaata gtgataaatt cgggaaaaaa
      601 gttaaaaaac ccc
//
LOCUS       DANLXF010000396          601 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.397, whole genome shotgun sequence.
ACCESSION   DANLXF010000396 DANLXF010000000
VERSION     DANLXF010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.397"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..296
                     /locus_tag="PXD66_002599"
     CDS             <1..296
                     /locus_tag="PXD66_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320834.1"
                     /translation="DTRYNKNGQGAVSERNIIDAKNRFKASHPYAKIISCVRGSETYV
                     TRPAKANATTKSVKEKQSQPQESSSLSSMLLGAAVTAGVGFVANKWFSKKDNT"
     gene            324..>601
                     /locus_tag="PXD66_002600"
     CDS             324..>601
                     /locus_tag="PXD66_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320835.1"
                     /translation="MAGLLPPYFKKKNSDHYKDAVKQFGKEQVDEVIAQCDELESWVK
                     EMPTKQLIMYVGCFHTFNKLSQSKAYVQENFPNVLENPFLFQTLSAAS"
BASE COUNT          216 a          102 c          111 g          172 t
ORIGIN      
        1 cagataccag atataacaag aatgggcaag gtgctgtttc tgaaagaaat attatagatg
       61 ctaaaaatcg ttttaaagcg agccatccat acgcaaaaat tatttcttgt gttagagggt
      121 ctgagacata tgtaacacgt ccagcaaaag ctaatgctac aacaaaatct gtcaaggaaa
      181 agcaatctca accacaagaa agttcatcgc tcagttcaat gttacttggt gcggctgtaa
      241 ctgctggggt tggttttgta gccaataaat ggtttagcaa aaaagataac acttaattta
      301 tctctaacca atgtggagta aaaatggctg gattattacc accttatttc aaaaagaaaa
      361 atagtgatca ctataaagat gctgtaaaac aatttgggaa agaacaagtt gatgaggtta
      421 tagcacagtg tgatgaactt gaatcttggg taaaagaaat gccaacaaaa caacttatta
      481 tgtatgttgg ttgtttccat acttttaaca aattatctca atctaaagct tatgttcaag
      541 agaactttcc aaacgtactg gaaaacccgt ttcttttcca aacactttcg gcagcgtctc
      601 a
//
LOCUS       DANLXF010000397          596 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.398, whole genome shotgun sequence.
ACCESSION   DANLXF010000397 DANLXF010000000
VERSION     DANLXF010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.398"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..>596
                     /locus_tag="PXD66_002601"
     CDS             <1..>596
                     /locus_tag="PXD66_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HDL6320836.1"
                     /translation="GNLSGVNQSNIKALQFFIYQSQQTEEPLQRRFENSEIYGPFVVK
                     SENHTYNQYFIKAVDAQKEWINTILDSPFIMAVLLMFAGIPLLIWLSFKITKPVKELT
                     ISANAVASGNLETNPKLEAEWIYEIGEVGKSFNHMVISLKNLTQQQQRMISDISHELK
                     TPLARLQLAIAILRRKTCGLAEIDRIEAEVGKLDQLIK"
BASE COUNT          215 a           93 c          109 g          179 t
ORIGIN      
        1 ggtaatttaa gtggcgtaaa ccaaagtaat attaaagcat tgcagttttt tatttatcaa
       61 tcacaacaga ctgaagagcc gttacaaaga cggtttgaaa atagtgaaat ctatggtcca
      121 tttgtcgtga aatccgaaaa ccatacttat aaccaatatt ttattaaagc ggtagatgct
      181 caaaaagagt ggattaatac gattttagac tcacctttta ttatggcagt attacttatg
      241 tttgccggaa ttcctttatt aatatggtta tcctttaaaa ttaccaaacc ggttaaagaa
      301 ttaactatct cggcaaatgc ggtagcctct ggtaatttgg aaactaatcc taaattagaa
      361 gcagaatgga tctatgaaat tggggaagtt ggaaaaagct ttaatcatat ggtgataagc
      421 ttaaaaaact taactcagca gcaacaaaga atgatttcgg atatatctca tgagttaaaa
      481 acacctttag ccagattaca actcgctatt gctatattac gccgtaaaac ctgtggtctg
      541 gcagaaatcg atcgcattga agcagaagta ggtaaattag accaattgat taaaga
//
LOCUS       DANLXF010000398          593 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.399, whole genome shotgun sequence.
ACCESSION   DANLXF010000398 DANLXF010000000
VERSION     DANLXF010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.399"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..>593)
                     /locus_tag="PXD66_002602"
     CDS             complement(<1..>593)
                     /locus_tag="PXD66_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015483985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6320837.1"
                     /translation="RILVDAYYEDGTTGGGEFVADLQDLVTPDDGGVCFVVNNNGGRW
                     KRVDLSHLTLFDFWAVGDGVTNDESAFVNAMRYSQFFIENGTFRINNAVNSVRDNVKI
                     LGNKTGKLVLGAGIQQAGAEVFNINHSNYFISGFCIETPNKAIGIRFKSLDDAGVKNL
                     HIDNVVFNGTFYGVRAGESIQADTNYPTDNVIVQNCQS"
BASE COUNT          193 a          150 c           89 g          161 t
ORIGIN      
        1 gattggcagt tttgtacaat cacattatca gttgggtaat tagtatccgc ctgaatactt
       61 tcacctgccc tgaccccgta gaaggtgcca ttaaacacaa cattgtcaat atggagattt
      121 ttaacaccag catcatccaa agacttaaat cgaataccaa ttgctttgtt cggtgtttca
      181 atacaaaaac ccgagataaa ataattgctg tgattaatat taaaaacttc ggcccctgct
      241 tgctggatac ctgcacctaa tactaatttg ccggtcttat tgcccaaaat tttcacatta
      301 tcgcgaacag aattaacggc attgttgata cgaaatgtac cgttttcgat aaaaaattga
      361 gaatatctca tagcatttac aaatgctgat tcatcatttg ttaccccgtc accgacggcc
      421 caaaaatcaa ataatgtaag atgtgaaagg tcaacacgct tccaccgccc cccattatta
      481 ttcacaacaa aacaaacccc accatcatca ggcgtaacta aatcctgcaa atcagccaca
      541 aactccccac cgcctgttgt gccgtcctca tagtaagcat ccaccaaaat acg
//
LOCUS       DANLXF010000399          591 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.400, whole genome shotgun sequence.
ACCESSION   DANLXF010000399 DANLXF010000000
VERSION     DANLXF010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.400"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            12..272
                     /locus_tag="PXD66_002603"
     CDS             12..272
                     /locus_tag="PXD66_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005820401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bor family protein"
                     /protein_id="HDL6320838.1"
                     /translation="MATVFSLAACSTQTLELSPAKEAQYDRVQHFFVSGLAQKQEIDA
                     AKICNGADKVGKVETETTFFNGLLGTITYGIYSPRQIRVYCN"
     gene            297..>591
                     /locus_tag="PXD66_002604"
     CDS             297..>591
                     /locus_tag="PXD66_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bor family protein"
                     /protein_id="HDL6320839.1"
                     /translation="MKKLLAMSVLTALLTTACTTQTAHIGGKVTDNIKPTKSVTQSFF
                     IGGIGQEETLNVVEVCGSKDKVQQVETVLSGGDILLGLVTIGIYTPRTANVYCK"
BASE COUNT          186 a          100 c          119 g          186 t
ORIGIN      
        1 aattacttgc aattgcaaca gtattttctt tagcagcctg ttctactcaa acacttgaac
       61 tatctcctgc taaggaagct caatatgatc gtgttcaaca cttctttgtt tcagggcttg
      121 ctcaaaaaca agagatagat gcagcaaaaa tttgtaatgg tgcagataag gtaggtaagg
      181 tagaaactga aactactttc tttaatggct tgttaggtac aattacttat ggtatctatt
      241 cacctcgcca aatccgtgta tattgtaact aatcctagta atttaaggag ttgaaaatga
      301 aaaaattgct tgctatgtct gtattaacag ccctgctgac tacagcatgt acaacacaaa
      361 ctgcccatat tggcggcaaa gttactgata atattaaacc aactaagagt gtaactcagt
      421 cgttctttat aggtgggatt gggcaagaag aaacattgaa tgttgtagag gtttgtggtt
      481 ccaaagataa agtgcagcag gttgaaacag tactttcagg tggggacatt ctattaggtc
      541 ttgtgactat cggtatttat acacctcgta ctgcaaatgt atattgtaag t
//
LOCUS       DANLXF010000400          589 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.401, whole genome shotgun sequence.
ACCESSION   DANLXF010000400 DANLXF010000000
VERSION     DANLXF010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.401"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..>589
                     /locus_tag="PXD66_002605"
     CDS             <1..>589
                     /locus_tag="PXD66_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6320840.1"
                     /translation="FAENANRRTIKVTSTGTNGPVGIVSSNRHSTSYFQQGETYTLSL
                     FARGSKALDYLYLMRQDGNNVRLPVINVASETEFNHYKLTFKAPFTTQQGYVLIGFRQ
                     TSTEQFVEFHSVKLEKGNVATDWTPAPEDVESAVSAVSADLTSYKQTQAATDSAQAQQ
                     LNQLSVNLTKAETNFNAKITEEKKARVDADKVNAD"
BASE COUNT          194 a          126 c          118 g          151 t
ORIGIN      
        1 aatttgctga aaatgcaaat cgccgaacta tcaaagtaac ctcaacaggt actaacggtc
       61 ctgttggtat tgtttcatct aatcgccatt ccacatctta tttccaacaa ggagaaactt
      121 atacactctc attatttgct cgaggaagta aggcactaga ctatctctat ctaatgcgtc
      181 aagatgggaa taacgttaga ttaccagtga ttaacgttgc ctctgaaact gagtttaatc
      241 attacaaact cacgttcaag gctcctttta ccacgcaaca aggttatgtc ttaatcggtt
      301 ttcggcaaac cagcacagag cagtttgttg agtttcacag cgtcaaactt gaaaaaggca
      361 acgtagccac agactggaca ccagcaccgg aggatgtgga gagtgcggtt agtgctgttt
      421 cggctgattt aacgagttat aaacaaactc aagcggctac cgattcagca caagcacagc
      481 aacttaatca gttatctgtg aacttgacca aagcagagac taatttcaac gccaaaatca
      541 cggaagaaaa aaaagctcgt gttgatgcag ataaagtgaa tgcagataa
//
LOCUS       DANLXF010000401          581 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.402, whole genome shotgun sequence.
ACCESSION   DANLXF010000401 DANLXF010000000
VERSION     DANLXF010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.402"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..298
                     /locus_tag="PXD66_002606"
     CDS             <1..298
                     /locus_tag="PXD66_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL6320841.1"
                     /translation="ACLIYLRKFGYSLGRPYADTVYQSKFPNMKELRIQNKGKPIRAF
                     YAFDPLRQAIVLCAGEKGKDKRFYEKMIALADAEFTSYLNNLEKENENVRSNAQ"
     gene            270..569
                     /locus_tag="PXD66_002607"
     CDS             270..569
                     /locus_tag="PXD66_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL6320842.1"
                     /translation="MKTLDQMLNELSPERRQKVEDMAQEMIREIQLSKLREELNLSQQ
                     QLATALGISQPAIAKLEQKDNDPRLSTLKRYIEAMGGKLKLAVELPTGDERVIRI"
BASE COUNT          201 a          107 c          111 g          162 t
ORIGIN      
        1 ggcttgttta atctatctgc ggaaattcgg ttatagtcta ggcagacctt atgctgatac
       61 agtttatcag tctaaattcc ctaatatgaa agagttacgc attcaaaaca aaggtaagcc
      121 aattcgagca ttttatgctt ttgacccttt acgccaagcg atagtacttt gtgctggcga
      181 gaaaggtaaa gacaaaagat tctatgaaaa aatgatcgct cttgctgatg cggaatttac
      241 ctcctattta aataatttag agaaagaaaa tgaaaacgtt agatcaaatg ctcaatgaac
      301 tctctcctga acgccgccaa aaagtggaag atatggctca agaaatgatt agagagatac
      361 aactttcaaa actaagagaa gagctaaatc tctctcaaca acaattagct acagctctcg
      421 gtatttcaca gcctgctatt gctaaacttg aacagaaaga taacgatcca agattatcca
      481 cgctcaaacg ctatattgaa gcaatgggcg gtaagttaaa acttgccgtt gaattgccta
      541 caggcgatga gcgtgtaatt aggatttaat tactttgtta a
//
LOCUS       DANLXF010000402          568 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.403, whole genome shotgun sequence.
ACCESSION   DANLXF010000402 DANLXF010000000
VERSION     DANLXF010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.403"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..524
                     /locus_tag="PXD66_002608"
     CDS             <1..524
                     /locus_tag="PXD66_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320843.1"
                     /translation="TMALAACDQAKEAAQSTTDKAVEVKEAVAEKAAEVKSATEAKVE
                     ELKAAANDAAQATADKTEEAKKAVAEKATELKTTTEAKVEELKAAANDAAQATTDKTE
                     EAKKAVAEKAAELKATTEAKVEELKAAASDATQATADKAEEAKKAVAEKAAEVKVETE
                     TKVEEMKKAIESK"
BASE COUNT          247 a           95 c          142 g           84 t
ORIGIN      
        1 caactatggc acttgctgct tgtgatcagg caaaagaagc cgcgcaatca actacagaca
       61 aagcggttga agtaaaagaa gctgtagctg aaaaagctgc agaagttaaa tcggctactg
      121 aagcgaaggt tgaagagttg aaagcggcag caaatgatgc agctcaagca acagcagata
      181 aaacagaaga ggcaaaaaag gcagtggcag agaaagccac tgagttaaaa accacaactg
      241 aagctaaagt ggaagaacta aaagcagcag caaatgatgc agctcaagca acaacagata
      301 aaacagaaga ggcaaaaaag gcagtagcag agaaagctgc tgagttaaaa gccacaactg
      361 aagctaaagt ggaagaacta aaagcagcag caagtgatgc gactcaagca acagcagata
      421 aagcagaaga agctaaaaag gcagtagctg agaaggcagc agaagtgaaa gttgagacag
      481 aaacaaaagt tgaagaaatg aaaaaagcga ttgaaagcaa atagtgcttt ttagtttctc
      541 tgtagctatc gaaacacgct aaaaataa
//
LOCUS       DANLXF010000403          567 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.404, whole genome shotgun sequence.
ACCESSION   DANLXF010000403 DANLXF010000000
VERSION     DANLXF010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.404"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..449)
                     /locus_tag="PXD66_002609"
     CDS             complement(<1..449)
                     /locus_tag="PXD66_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL6320844.1"
                     /translation="MPNFSFTHLYKKHTKELPTVTLLSALIGLSQVSYANPLPLGNTT
                     GTNNLAVGEGSFALGTGSQAIGKNAIATGGNISPQEFNVELENFRDLVNQIEELRTQI
                     AEQEKQGSVNQQLQDALKSQIAQYDTILERVREKQAQQAILANSVIS"
BASE COUNT          168 a           94 c          109 g          196 t
ORIGIN      
        1 attgatataa cactattggc taatattgct tgttgtgctt gtttttcacg tacacgctct
       61 aaaatagtgt catattgagc aatctgactc tttaaagcat cttgtaattg ttgattaaca
      121 gagccttgtt tttcttgttc agcaatttga gtacgtaact cttcaatttg attaactaaa
      181 tcacggaagt tttctaactc cacattgaat tcctgggggg agatattacc accggttgca
      241 atggcattct tgccgattgc ttgagaacct gtgcctaacg caaaactacc ttcacctaca
      301 gcaaggttat tggtgccagt ggtgttgcct aaaggaagtg gattggcata agagacttgg
      361 ctaagcccta tcagagcgct aagcaaggta acagtaggga gctcttttgt gtgtttttta
      421 tacagatggg tgaaactaaa gtttggcata tcaaaatttc ctcattaaaa cttttacttt
      481 tcattcttaa aaaaatgctc cgtgtcataa tatttaattt gattaaatga aataggaaaa
      541 gattaaattt gtgaaaatat tttgagt
//
LOCUS       DANLXF010000404          562 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.405, whole genome shotgun sequence.
ACCESSION   DANLXF010000404 DANLXF010000000
VERSION     DANLXF010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.405"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..562)
                     /gene="nfuA"
                     /locus_tag="PXD66_002610"
     CDS             complement(<1..562)
                     /gene="nfuA"
                     /locus_tag="PXD66_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005712445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HDL6320845.1"
                     /translation="MHISISDAAQAHFARLLEQQEEGTNIRIFVVNPGTPGAECGVSY
                     CPPNAVEETDTEFKFNRFSAFVDDISLPFLDEAEIDYVTDPMGSQLTLKAPNAKMRKV
                     ADDAPFIERLDYVIQTQVNPQLASHGGKVTLIEITEDKYAVLQFGGGCNGCSMVDVTL
                     KEGIEKQLLAMFPDELVGVKDITEHQH"
BASE COUNT          147 a          124 c          122 g          169 t
ORIGIN      
        1 cgtgttggtg ttcggtaata tctttcacac caactaattc atcagggaac attgccagaa
       61 gctgtttctc aattccctct tttaaggtca catccaccat tgagcaaccg ttacaaccac
      121 cgccgaattg tagcacggca tatttatctt cggtgatttc aattaaggtc actttaccgc
      181 cgtggctggc aagttgtgga ttgacttggg tttgaatcac ataatctaaa cgctcgataa
      241 acggtgcatc atcagccact ttacgcattt tggcgttagg ggctttgagg gtaagttgtg
      301 aacccattgg gtcggttaca taatcaattt ccgcttcgtc taaaaacggt aagctaatat
      361 catcgacaaa tgcagagaaa cggttaaatt taaattcggt atccgtctct tcaactgcat
      421 tgggtgggca gtaagagaca ccgcattctg cccccggtgt gccgggattg accacaaaaa
      481 tacggatatt agttccctct tcttgctgtt ctaatagtct ggcaaaatgg gcttgtgctg
      541 catctgatat tgaaatatga at
//
LOCUS       DANLXF010000405          541 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.406, whole genome shotgun sequence.
ACCESSION   DANLXF010000405 DANLXF010000000
VERSION     DANLXF010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.406"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..>541)
                     /locus_tag="PXD66_002611"
     CDS             complement(<1..>541)
                     /locus_tag="PXD66_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015483985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6320846.1"
                     /translation="GHYLCTKVKGVRFFNNIAIGGRNVSAYGATSCSDIFILGNREQG
                     MAVTTVDVEAGVQVEDVQTGECVVANNICSHDIWMSGAKNAIINSNLCRRLRVTSGNP
                     TDTGSNNVEFSNNQAKSIAISKYGSFDNPSLFIDNINFINNILEPSEFNQDVYIDGTI
                     LKQCQFINNRCISKSKQYSV"
BASE COUNT          168 a          114 c           90 g          169 t
ORIGIN      
        1 tgacagaata ttgcttagac ttgctaatac aacgattatt aataaattgg cactgtttta
       61 aaattgtccc gtcaatatac acatcttgat taaattcact gggctccaaa atgttattaa
      121 taaaattgat attatcaata aacaatgaag gattatcgaa agaaccatat ttcgatattg
      181 caatagattt tgcttgatta tttgaaaatt ctacattgtt gctgcctgtg tcagtcggat
      241 tacctgatgt gacacgcaag cgacggcaga ggttgctgtt gataatggca tttttagcac
      301 cagacatcca aatatcgtgg ctgcaaatat tattcgctac cacacattcg ccagtttgta
      361 catcttccac ttgtacgccc gcttccacat caactgtcgt cactgccatc ccctgttcac
      421 gattacccaa aatgaaaata tcagagcaag atgttgcacc gtaagctgat acattgcgac
      481 ctccaattgc tatattgtta aaaaatcgca cacctttaac tttggtgcag agataatgtc
      541 c
//
LOCUS       DANLXF010000406          539 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.407, whole genome shotgun sequence.
ACCESSION   DANLXF010000406 DANLXF010000000
VERSION     DANLXF010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.407"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..>539
                     /locus_tag="PXD66_002612"
     CDS             <1..>539
                     /locus_tag="PXD66_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320847.1"
                     /translation="ERITPPYRSSFPQNLPTNEEIDKKYHSDGQRIIIRSMTTEYGRG
                     MCGYGDFGRYTFEAKFRNWESYKFGNLINVDLLSNYACQMIFEQFGYDDKKHLEFDKE
                     TRSRYWRRDDTNKIERIGKKYQWLALYEILARVMDNFPTFSNRYADEIGLEYITNLDN
                     FYLPKTQIKIVPPTKTDVF"
BASE COUNT          189 a           81 c          100 g          169 t
ORIGIN      
        1 agaaagaatt acgccacctt atagaagttc atttccccaa aacttaccaa ccaatgaaga
       61 aattgataaa aaatatcatt ctgatggaca aagaatcatt atccgttcaa tgacaactga
      121 atatggtcgt ggtatgtgtg gatatggtga ttttggcaga tatacttttg aagccaaatt
      181 ccgaaattgg gagagttata aattcggcaa tctcatcaat gttgatttat taagtaatta
      241 tgcttgccaa atgatttttg agcaatttgg ctatgatgac aagaagcacc ttgagtttga
      301 taaagaaact cgttcaagat attggcgaag agatgatact aataaaattg agcgaattgg
      361 caagaaatat caatggttgg ctttgtatga aatcttggca agggtgatgg ataattttcc
      421 tactttttct aatcgctatg ccgatgaaat agggcttgaa tatattacta atttggacaa
      481 cttttatcta cccaaaaccc aaattaaaat tgtaccgcca actaaaacag atgtatttt
//
LOCUS       DANLXF010000407          539 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.408, whole genome shotgun sequence.
ACCESSION   DANLXF010000407 DANLXF010000000
VERSION     DANLXF010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.408"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..494)
                     /locus_tag="PXD66_002613"
     CDS             complement(<1..494)
                     /locus_tag="PXD66_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320848.1"
                     /translation="MSALINLFYLYDPWFFHFLRMSFVVGLVACGYLVFQIIKGKSNG
                     IIIPKDSLIAIIALIGFSIIPLLINSTKEIGVLAMYVKMLILFVFGIAIYNVFYHNQQ
                     GKTLFVRDLKIGIAIQWICGVTALLGLPFMVDFLLSSNAMMPRFFGSEQEYRLYNITS
                     SAFF"
BASE COUNT          210 a           99 c           85 g          145 t
ORIGIN      
        1 tggaaaaagg cggaagaagt gatgttatat aaacggtatt cttgttccga accaaaaaaa
       61 cgtggcatca ttgcattgct ggataataaa aaatccacca taaacggcaa acctaataag
      121 gctgtcacac cacaaatcca ttgaatagca atgccgattt ttaaatcacg aacgaataaa
      181 gttttacctt gttggttatg ataaaaaaca ttataaatcg caatcccgaa cacaaacaaa
      241 atcagcattt tgacatacat tgccagcaca ccaatttctt ttgtactgtt aatcaataat
      301 ggaataatgc taaagccgat taatgcaata attgcaatca ggctatcttt aggaataata
      361 atgccattcg atttgccctt gattatttgg aaaaccaaat agccacaagc aacaagcccg
      421 actacaaacg acattcttaa aaaatggaaa aaccacggat cgtaaagata aaataagtta
      481 atgagtgctg acatttcaga cctattatga atttcgctga gtaaaattac gtttaattg
//
LOCUS       DANLXF010000408          536 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.409, whole genome shotgun sequence.
ACCESSION   DANLXF010000408 DANLXF010000000
VERSION     DANLXF010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.409"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..>536
                     /locus_tag="PXD66_002614"
     CDS             <1..>536
                     /locus_tag="PXD66_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL6320849.1"
                     /translation="LVTAIPNFLEKVNNDFAISARNRDLYSHYLPYQGIDDWENSNFN
                     FRTLNGVYYDDNLTSTELLRKFMIQRDYNTLYALSSARTGNADSLAKNYHLVVAKMSY
                     ENLKKELSKKPDSTFNKLKKLYETNGEFLNEYILDNFRGNPLELLTVPALKESLENQE
                     SFPSRLYQKFNIFNQAIA"
BASE COUNT          194 a           92 c           85 g          165 t
ORIGIN      
        1 ttagtaacag ccattcctaa cttcctggag aaagttaata atgattttgc tataagtgct
       61 agaaatagag acttatatag tcactacctt ccttatcagg gtattgatga ttgggagaat
      121 tctaatttta actttcgaac actaaatgga gtttattatg atgataatct aacttcaaca
      181 gaactcctgc gaaaatttat gattcaaaga gattataaca ctctttatgc tctaagttct
      241 gctcgcacag gaaatgctga tagtctggct aaaaattacc atcttgtagt tgcgaaaatg
      301 tcctatgaaa acctgaaaaa agaattatcc aagaaacccg actctacatt taataaactg
      361 aaaaagctgt atgaaaccaa tggtgaattc ttaaatgaat atattttaga caatttccga
      421 ggaaatccac ttgaattatt aacagtacct gcgcttaaag aaagtttaga aaaccaagag
      481 tcattcccta gcaggctgta tcagaaattt aatattttca accaggctat tgcaca
//
LOCUS       DANLXF010000409          532 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.410, whole genome shotgun sequence.
ACCESSION   DANLXF010000409 DANLXF010000000
VERSION     DANLXF010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.410"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            120..>532
                     /locus_tag="PXD66_002615"
     CDS             120..>532
                     /locus_tag="PXD66_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HDL6320850.1"
                     /translation="MHSILKKILGKSKSSSQSSVKSDNVFAIEQDSEGWLYPKNASQL
                     LNTELRQKYLGLLWQQVSMNRQMFEALYQQPIERYAEMVQLLPASESHHHSHIGGMLD
                     HGLEVIAFSAKVRQNYILPPNVAPEEQSKQRDAWTA"
BASE COUNT          163 a          120 c          108 g          141 t
ORIGIN      
        1 agggtaatcc cctaaggggg ataagagtaa cctttccaag gggaaaggtc ttacccaaaa
       61 gggcaaaggg ctaaacggct ttctttcaac gcaaacatca tctttttcgg tctatttcaa
      121 tgcactcaat tctcaaaaaa attcttggca aaagtaaatc ctcgtctcaa agttcggtta
      181 aatctgacaa tgttttcgct attgagcagg atagtgaggg ctggttatat ccgaaaaatg
      241 cctcccaact acttaatacg gaattacgcc aaaaatatct gggtctactt tggcaacagg
      301 tatcaatgaa ccgtcaaatg tttgaggcac tttatcaaca gccaatagag cgttatgccg
      361 aaatggtgca actgttaccc gcttccgagt cacatcatca ttcacatatc ggcggaatgc
      421 tcgatcacgg tttggaagtt attgcttttt cagccaaagt ccgtcaaaac tatatattgc
      481 caccgaatgt cgcacctgaa gaacaatcta agcaacgaga cgcttggact gc
//
LOCUS       DANLXF010000410          517 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.411, whole genome shotgun sequence.
ACCESSION   DANLXF010000410 DANLXF010000000
VERSION     DANLXF010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.411"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            36..461
                     /locus_tag="PXD66_002616"
     CDS             36..461
                     /locus_tag="PXD66_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL6320851.1"
                     /translation="MYKHILVAVDLSEESLVLVRKGAALAEKCGAKLSLIHVDVNFSD
                     LYTGLIDINMSSVQYSVVEETNTALAELASKVNYPVSECLNGTGDFSQVLEEAVEKHN
                     VDLLITGHHQDFWSKFMSSTRRVMNNITVDMLVVPLADE"
BASE COUNT          165 a           86 c          109 g          157 t
ORIGIN      
        1 caaagtaaac aactctcaca agcaaggagc atattatgta taaacatatc ttagttgcag
       61 tggatctttc tgaagaaagt cttgttttag tgcgtaaggg ggctgcgtta gcagaaaaat
      121 gcggagctaa actttccctc attcacgttg atgttaattt ttcggatctc tatacaggat
      181 taattgatat taatatgtct tcggtacaat acagtgttgt tgaagaaaca aatacggcac
      241 tggctgaact tgcctcaaaa gtaaattatc ctgtatctga atgtttaaac ggaacgggag
      301 attttagtca agttttggaa gaggccgtag aaaaacacaa tgtcgattta ctcatcactg
      361 gccatcatca ggacttctgg agtaaattta tgtcttcaac tcgtcgagtc atgaataata
      421 tcactgttga tatgctggtt gtgccattag cagatgaata gcaagaaagt gaatgaaaaa
      481 taaccgcttg agacagttta tctgtcttac aagcggt
//
LOCUS       DANLXF010000411          509 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.412, whole genome shotgun sequence.
ACCESSION   DANLXF010000411 DANLXF010000000
VERSION     DANLXF010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.412"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..>509
                     /locus_tag="PXD66_002617"
     CDS             <1..>509
                     /locus_tag="PXD66_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL6320852.1"
                     /translation="MPRGDKNAMKGFIFNIPSIEYQKKCIANYFAFDQKIQLNTQTNQ
                     TLEAIAQAIFKSWFVDFDPVRAKAAALSEGKSEHEANLAAMSVICGKDTSELNDTEYK
                     ALWQIAEAFPSEFGDEGLPIGWKFNQADNLFDVGIGKTPPRKESEWFSDNANDTEWIS
                     IKDMGNQGL"
BASE COUNT          177 a          101 c          109 g          122 t
ORIGIN      
        1 aatgccacga ggggataaaa atgcaatgaa aggttttatt ttcaatatcc caagtattga
       61 atatcaaaaa aaatgtattg ctaattattt tgcctttgac caaaaaatcc aactcaacac
      121 ccaaaccaac caaaccctag aagccattgc acaggcaatc ttcaaaagct ggtttgtgga
      181 ttttgaccct gtgcgcgcca aagccgccgc cctaagcgaa ggcaaaagcg aacacgaagc
      241 caaccttgcg gcaatgtcgg tgatttgtgg taaagacacc agcgagttaa acgacaccga
      301 atacaaagcg ctttggcaaa tcgccgaagc cttcccaagt gagtttggag atgaagggtt
      361 acctattggc tggaaattca atcaagcaga caatttattt gatgttggta ttgggaaaac
      421 cccaccaaga aaagagagtg aatggttttc tgataatgca aatgatacag aatggatttc
      481 tattaaagat atgggaaatc agggattat
//
LOCUS       DANLXF010000412          506 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.413, whole genome shotgun sequence.
ACCESSION   DANLXF010000412 DANLXF010000000
VERSION     DANLXF010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.413"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..>506
                     /locus_tag="PXD66_002618"
     CDS             <1..>506
                     /locus_tag="PXD66_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HDL6320853.1"
                     /translation="LETTANKIKRSKDDSIEWHRKNEKEIPFWVITETWDFGALSKFY
                     SLLNGQHQRKIAKKFNVDENTLKNWLNEINIVRNLSAHHSRIWNRKFSDIAIPKLDDP
                     RWSKFKKMQQYFSNLSLDDKAKERIFGRVAILWFLVSQTSTNYHWLDKFEDLLNDFPN
                     LPNTKVEL"
BASE COUNT          184 a           69 c           97 g          156 t
ORIGIN      
        1 gctagaaact acagctaata aaattaagcg tagtaaagat gattctattg aatggcatag
       61 aaaaaatgag aaggaaatcc ctttttgggt aattactgaa acctgggatt ttggtgcatt
      121 atctaagttt tactcattgt taaatgggca acaccaaagg aaaattgcta agaaatttaa
      181 tgtagatgag aatacgttaa agaattggtt gaatgaaata aacattgtaa gaaacctttc
      241 tgctcaccat tctcgtattt ggaacagaaa atttagcgat attgctattc ctaagctaga
      301 tgatcctcgt tggtcgaaat ttaaaaaaat gcagcagtat ttttcaaact tatcgctgga
      361 tgacaaagca aaggagagaa ttttcggaag ggtagctatt ctgtggtttt tagttagtca
      421 aaccagcact aattaccatt ggttagataa gtttgaagat ttattaaatg acttccctaa
      481 tttaccgaat acaaaagtag aactga
//
LOCUS       DANLXF010000413          502 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.414, whole genome shotgun sequence.
ACCESSION   DANLXF010000413 DANLXF010000000
VERSION     DANLXF010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.414"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(181..420)
                     /locus_tag="PXD66_002619"
                     /pseudo
     CDS             complement(181..420)
                     /locus_tag="PXD66_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651213.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
BASE COUNT          158 a          104 c           70 g          170 t
ORIGIN      
        1 aaaataatgg cataagtgac tcacttatgc cattttttat atgctttaac gtttattaaa
       61 aatcttaaaa atattgttgg cttcctctaa ataatgcaag attttccgca ctcatattgc
      121 cgttaccaac acctgctacg acaatacctt gatagcctac atctaacaag gctttagtgg
      181 gccaattttc acaatctgct cacctttgat attcgccaat gttttcattt ctggcactgc
      241 ttcaattaag gtttcgatat tcaattgccc tgcttgataa gcactactat ttgcattctg
      301 ctgactacca gcaatcgttc cgcctgtcgc taaaatagtg atattaggta attctgccgc
      361 attagctgtt acaaataagc cacctaacat tgctgctaat gctaattttt tgaatttcat
      421 ataaggctcc ttaattaaaa aaactaaaga cagataatac cgataaatca atgtaaatta
      481 tttgatctga tacataaaag cg
//
LOCUS       DANLXF010000414          502 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.415, whole genome shotgun sequence.
ACCESSION   DANLXF010000414 DANLXF010000000
VERSION     DANLXF010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.415"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..356
                     /locus_tag="PXD66_002620"
     CDS             <1..356
                     /locus_tag="PXD66_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320854.1"
                     /translation="GVIQKHRFYEPDFTTIKNGKVIGLTPEFQLLRLPKPEFADALIN
                     LAKRVSIALPTPFARVDLYWDGKQAKVGEITLTPFYGYPYKWTLSQDIIMGRMWLEAL
                     DRMGKKLEDSFDWGN"
BASE COUNT          154 a           76 c          104 g          168 t
ORIGIN      
        1 aaggtgtaat tcaaaaacat cgattttacg agcctgattt taccactatt aagaatggaa
       61 aagttattgg cttaactcca gaattccaat tattacgttt acctaaacct gaatttgccg
      121 atgctttgat taatctagca aaacgagtat ctattgcatt acctacccca tttgctcgag
      181 tggatttata ttgggatgga aagcaagcta aagttggaga aattacatta actcctttct
      241 atggttatcc atacaaatgg acattatcac aggatatcat aatgggaaga atgtggttgg
      301 aggcactaga tagaatgggg aaaaaattgg aagattcttt cgattgggga aattaatgtg
      361 aaatcaacaa aggtatcgtt ctttcaggct atagcgatta gttttggtgc tattattggt
      421 tggggggctt atgtgatgcc tggagacctt tttctttcaa aaggtactct attaagctct
      481 tctcttgcta ttattttagg aa
//
LOCUS       DANLXF010000415          502 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.416, whole genome shotgun sequence.
ACCESSION   DANLXF010000415 DANLXF010000000
VERSION     DANLXF010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.416"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            35..>502
                     /locus_tag="PXD66_002621"
     CDS             35..>502
                     /locus_tag="PXD66_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDL6320855.1"
                     /translation="MHLNFKKIDNSHIALFYNVLVATFFVTVLTFKKGYSYVPMALGI
                     IATLSFLFYRFKLKQKWLLDKEDKFFIFSLIGYFLTFAMSAIFNGDGIREIDNPIQIL
                     LLIPLIILFRLYPIKKETVLHFIPIGALVAGCIAIYQRFILNLKFPFPEIMRIQ"
BASE COUNT          147 a           82 c           75 g          198 t
ORIGIN      
        1 atgacagcaa ttttcattgt tttaggatat gtcgatgcac cttaacttca aaaaaataga
       61 caactcacat attgctcttt tctataatgt actagtcgcc acattttttg tcactgtttt
      121 aacctttaag aaaggttata gttatgtgcc aatggcatta ggtattatcg ctacacttag
      181 tttcttattt tatcgtttca aattaaaaca aaagtggtta cttgataaag aggataaatt
      241 ttttattttc tcgcttattg gctattttct aacgtttgca atgtctgcta tttttaatgg
      301 cgatggcatt cgtgaaattg ataatcctat ccagatttta ttgcttatcc ctttaatcat
      361 cctctttaga ctttacccga ttaaaaaaga aactgtattg cactttatac cgattggagc
      421 gttggtagct ggttgtatag caatatatca gaggtttata ttaaatctta aatttccttt
      481 ccctgaaata atgcgtattc aa
//
LOCUS       DANLXF010000416          502 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.417, whole genome shotgun sequence.
ACCESSION   DANLXF010000416 DANLXF010000000
VERSION     DANLXF010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.417"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..230
                     /locus_tag="PXD66_002622"
     CDS             <1..230
                     /locus_tag="PXD66_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320856.1"
                     /translation="VANGIVNEPSVDCFSQEIAQFTGCHIFSSLYIAFNTQRSFKSHW
                     DSRDIFAVQMQGKKRWIIHTPTFKKTIVYAS"
     gene            220..>502
                     /locus_tag="PXD66_002623"
     CDS             220..>502
                     /locus_tag="PXD66_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HDL6320857.1"
                     /translation="MHRSKDMPEYAPNLDDVYMDIVLEAGDVLYLPRGWWHDPIPVGE
                     ETVHLAVGIFPAYTHNYLTWVSQNMVEKEIARASLSHYESDKELIAQLAE"
BASE COUNT          149 a           79 c          101 g          173 t
ORIGIN      
        1 tagtcgcaaa tggtattgta aatgaaccaa gtgttgattg cttctctcaa gagatagctc
       61 aatttacagg ctgtcatatt ttttcaagtt tatatattgc gtttaatact caaagatctt
      121 ttaaaagcca ttgggatagt cgtgatattt ttgcagttca aatgcagggt aaaaagcgtt
      181 ggattattca cactccaaca tttaaaaaaa ccattgttta tgcatcgtag taaagatatg
      241 cctgaatatg ctcctaatct tgatgatgta tatatggata ttgtattaga agctggagat
      301 gttctttatt tacctcgtgg ctggtggcac gatcctattc ctgttggaga agaaactgtg
      361 catttagctg taggcatttt tcctgcttat actcataact atctgacttg ggtatctcaa
      421 aatatggttg agaaagaaat tgcccgagct tctttatcgc attatgagtc agataaagag
      481 ttgatagcac agttggcaga ac
//
LOCUS       DANLXF010000417          501 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.418, whole genome shotgun sequence.
ACCESSION   DANLXF010000417 DANLXF010000000
VERSION     DANLXF010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.418"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
BASE COUNT          178 a           93 c           78 g          152 t
ORIGIN      
        1 aaagattata ctcttttagt acatcaacta attcatcagg taaattttct aacttccatc
       61 ttcgtttttc taaaatcgaa aagtaatact ggtcatcgtc taaaatctca atatatgtac
      121 ttccaaagag aacttttgta taaggcgtat catgatagat cgtgacatca gccttcaatt
      181 tgtactcgcc ttccatgcat tccccaaaat ggtattgact aaaaacaatg gttatgatat
      241 aatgacatgg ggcttgaaaa gcaagctgat tgctcaatag gtaatcagac cactttgaaa
      301 gctcggtcct tttcaaaaga aaaggtctta ctaattactc acaaaatctg gagaacaata
      361 tgaaaaacac tacagcgcaa caaaagccaa tgaaagttac agctaaacat attagagaat
      421 ctactactcg ttgttaagag cttagctttg atataaaaag accaactttt tatatcggcg
      481 tagtaggtct aatttgagcc t
//
LOCUS       DANLXF010000418          500 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.419, whole genome shotgun sequence.
ACCESSION   DANLXF010000418 DANLXF010000000
VERSION     DANLXF010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.419"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(205..366)
                     /locus_tag="PXD66_002624"
     CDS             complement(205..366)
                     /locus_tag="PXD66_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007525341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL6320858.1"
                     /translation="MEKIENSSNLVLKLSLENQMLILESLEHPKEPNLVMLEALKKYS
                     LYVAKTSNK"
BASE COUNT          155 a           83 c           83 g          179 t
ORIGIN      
        1 agagcataaa atgctctaac tgttctagtc tcttatctgt ttcctttttt gactcaaaac
       61 gcttttgctt aaaacgaact tctatcgaat ctacaccgat gtaaattcga tgttcctgca
      121 ttttgagtag tcggacagtt tcaaaaaaat gtttaggggt gatgtgagaa agggttatat
      181 ttgccatagt ttagcttgaa aacgctattt attacttgtt ttagcaacat acaaagaata
      241 cttttttaaa gcctctagca ttactaaatt aggctcttta ggatgttcta agctctcaag
      301 aattagcatt tgattttcaa gagaaagctt tagaacaaga tttgaactat tctctatttt
      361 ttccatttta attatctcat tagatgactt tcgcataaca acaggttatg tgtaaattct
      421 cgtgcagtgg gacaagcccg attgtactgc actcgaattg taacataacc tgtaggacat
      481 tatgcgaaaa ttaataatat
//
LOCUS       DANLXF010000419          500 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.420, whole genome shotgun sequence.
ACCESSION   DANLXF010000419 DANLXF010000000
VERSION     DANLXF010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.420"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..282
                     /locus_tag="PXD66_002625"
     CDS             <1..282
                     /locus_tag="PXD66_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDL6320859.1"
                     /translation="IIDELGDDKAVIAQKEIQAEIALCKRIFKQLDWSAVDFRIVKRL
                     YRKGFSENEIAVALVRFTDFEDRHCDSHDYLTRTITKAIQNYQQCSKAC"
BASE COUNT          192 a           68 c           94 g          146 t
ORIGIN      
        1 attattgatg agcttggaga tgataaagca gttattgcac aaaaagaaat tcaagcagaa
       61 attgctttgt gtaaacgtat attcaaacaa ttagattggt ctgctgttga ttttagaatt
      121 gttaagcgtc tgtacaggaa aggattttca gaaaatgaaa ttgctgttgc acttgtcaga
      181 tttactgatt ttgaagacag acattgtgat tcacatgatt atttgacaag aacaatcaca
      241 aaagccattc aaaactatca acaatgctca aaagcttgtt agatgttata aggaaagtta
      301 aaaatcaaca aaagatttga caaatttata taaaatcctt attatatatg aaaaggaagc
      361 gtagccttaa aacgcaaatg tgggttagca catggtcaat ctgaaactaa tctaggatat
      421 ttaaggtcta gcagatggca agcatcagaa aaacttgaaa ctaatctagg atatttaagg
      481 cctagcagat ggcagacatc
//
LOCUS       DANLXF010000420          499 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.421, whole genome shotgun sequence.
ACCESSION   DANLXF010000420 DANLXF010000000
VERSION     DANLXF010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.421"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(31..291)
                     /locus_tag="PXD66_002626"
     CDS             complement(31..291)
                     /locus_tag="PXD66_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320860.1"
                     /translation="MPRNELTKNARAIADLIHRKSATVTHKKLARAIGLSESQFSRTF
                     ADNVEMVAVIIDYLSIELADKDELLELKEKRYKRINLEEATT"
     gene            complement(312..482)
                     /locus_tag="PXD66_002627"
     CDS             complement(312..482)
                     /locus_tag="PXD66_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL6320861.1"
                     /translation="MSQAQLAVQIGWGQPRIANYETGNRTPSIYVAQKIVEGLNELGA
                     NVRIDDVFPVGN"
BASE COUNT          123 a          112 c           84 g          180 t
ORIGIN      
        1 ttctaaaaaa tttttcattg ttggtaactt ctatgttgtc gcttcttcga gatttattcg
       61 cttatatcgt ttctctttca gctctagtaa ctcgtcttta tccgcaagct caatacttaa
      121 gtaatcaata atcaccgcca ccatttccac attatctgca aatgtacggc taaattgtga
      181 ttcactcagt ccgattgctc ttgctagttt cttatgcgtc accgttgcgg attttcgatg
      241 aatcaaatcc gcaatcgctc ttgcattttt tgttaattca ttgcgtggca ttgcaagcct
      301 cttggggtaa attagttgcc aacggggaaa acgtcatcaa ttcgtacgtt tgcacctaat
      361 tcgttcaatc cctcaacaat tttttgagca acgtaaatag atggggtacg atttccagtt
      421 tcatagttag ctattcttgg ttgcccccaa ccaatctgaa cagctaactg agcttgactt
      481 ataccaattt ggtttcgta
//
LOCUS       DANLXF010000421          491 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.422, whole genome shotgun sequence.
ACCESSION   DANLXF010000421 DANLXF010000000
VERSION     DANLXF010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.422"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            3..>491
                     /locus_tag="PXD66_002628"
     CDS             3..>491
                     /locus_tag="PXD66_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005710811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1439 domain-containing protein"
                     /protein_id="HDL6320862.1"
                     /translation="MLSANANLLTISEQEINDYLATKLAEKIPLKDKVGIPKLFELDY
                     RLHSLTSQIGRTEEKKVAISGVVDGLLNAKGKKYAASIHLNMDTTPYYDPQKGALFLK
                     DVRLLSWSATPAKYQAELQMFLPVLADGLSSFLNSTPVYTLDENKTKEAMVKKFGKAI
                     VVE"
BASE COUNT          167 a           86 c           95 g          143 t
ORIGIN      
        1 ctatgctctc tgctaacgct aatttgctca ctattagcga gcaggaaatc aacgactatt
       61 tagccaccaa attagctgaa aaaattcctt taaaagataa agtcggtatt cctaaattat
      121 ttgaattgga ttatcgcctg cattcactta ccagccaaat tggcagaacc gaggaaaaaa
      181 aagtagctat ttctggcgtt gttgatggac ttttaaacgc aaaaggcaag aaatatgctg
      241 cctctatcca tttaaatatg gataccacgc cttattatga tccacaaaaa ggggcattat
      301 ttcttaaaga tgtcagattg ttaagttggt ctgcaacacc ggctaaatat caagcagagc
      361 tgcaaatgtt tttgccggtg ttggctgatg gattaagtag ttttttaaac agtacgcctg
      421 tttatacttt agatgaaaat aaaacaaaag aagcaatggt gaaaaagttc ggcaaagcca
      481 ttgtagtaga a
//
LOCUS       DANLXF010000422          486 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.423, whole genome shotgun sequence.
ACCESSION   DANLXF010000422 DANLXF010000000
VERSION     DANLXF010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.423"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
BASE COUNT          160 a           76 c           99 g          151 t
ORIGIN      
        1 gaatgaaatg aaagaaccat caattttaca attaagctta gtagttggag ggaattctga
       61 aaataaggct aatcaaccag atcttgtatc ttatggcgtt gcttttgcta cagggtttgc
      121 aagagggggg ctttaggcgg tttatccagt acttctgtta atttagccct tgatttagcg
      181 aggagcctac ctattacaat acatgaaaat tctttaccta gcggtgtaaa agaattaatt
      241 ccatcatctt taccaataaa gcctacgttt ggagttccag gtagaacgaa taataaccaa
      301 tctggctcaa actacgattg tggtgatggt acagattatc aaggctagtg gagatgaaaa
      361 tgaaaattca atttccaatt tcttatcaag aatttcgtga aagttattct gagaaacaac
      421 cacttttaat gaaaggtgct atagagtagc aagatttact ttcttggaag gctatcaatg
      481 aagtat
//
LOCUS       DANLXF010000423          479 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.424, whole genome shotgun sequence.
ACCESSION   DANLXF010000423 DANLXF010000000
VERSION     DANLXF010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.424"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
BASE COUNT          161 a           66 c           95 g          157 t
ORIGIN      
        1 gattgttaag tggttatggg ctgaattttg ggataagaat attatctcga tgacacttag
       61 aattgatggt tatagaaatg ctaacttatg gcattattta cgttatcaat cacggattga
      121 ggtattgtat aaaatagcaa atgaacaccc gaatctacta ccattattag cctatgttaa
      181 acctgagtac tggaaatgct cggacttatt tagttatcaa aattgggtaa gaagccgtga
      241 taaacagcct tttattttgt atgctaaatg ccaagataag atgttatgtg aatcaaaggg
      301 gcaatggaga tggttaaaaa aacagtcgag aatagttgta gatcaatgtt ttctacatgt
      361 taatcaatgg cagttattaa cagaaattca aatgcctttc aagatcccag tatgtgtcat
      421 atgtttcata cttactaatt ttagcatgat cagctgtgag ttagatcgat ctagacaac
//
LOCUS       DANLXF010000424          455 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.425, whole genome shotgun sequence.
ACCESSION   DANLXF010000424 DANLXF010000000
VERSION     DANLXF010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.425"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..>455)
                     /locus_tag="PXD66_002629"
     CDS             complement(<1..>455)
                     /locus_tag="PXD66_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595-like element ISMha2 family transposase"
                     /protein_id="HDL6320863.1"
                     /translation="QHKLIELFVAGVTARTAAELVNVNKTTAAYYFHRLRLLIYQHSP
                     HLEMFEGEIEADESYFGGARKGKRGCGAAGKTAVFGLLKRDDKVYTVVVPNTQSATLL
                     PIIREKVKPDSLVYADFYRSYDVLDVSEFNHFRINHSTHFAENHNHING"
BASE COUNT          126 a          104 c           96 g          129 t
ORIGIN      
        1 attccgttaa tgtgattgtg attttcagcg aagtgtgtgc tgtgattgat acgaaaatgg
       61 ttaaattcac tcacatcaag cacatcatag cttcgataaa aatcggcgta aacaaggctg
      121 tctggcttca ctttctcccg aataatcggc aaaagtgtgg cagattgcgt atttggtacg
      181 acaacggtat aaaccttgtc atcacgtttt aacagcccaa atacggcagt tttgcctgcg
      241 gcaccacacc ctcgtttacc tttgcgagca ccaccgaaat aactttcgtc cgcttcaatt
      301 tcaccctcaa acatttccag atgagggcta tgttgataga tgagcaatcg caaacggtga
      361 aagtaatagg cagcggtggt tttgtttaca ttcaccaact cagccgctgt tcgagcagtg
      421 acacctgcta caaacagttc aatgagttta tgttg
//
LOCUS       DANLXF010000425          455 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.426, whole genome shotgun sequence.
ACCESSION   DANLXF010000425 DANLXF010000000
VERSION     DANLXF010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.426"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..>455)
                     /locus_tag="PXD66_002630"
     CDS             complement(<1..>455)
                     /locus_tag="PXD66_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320864.1"
                     /translation="RHDDFRELAKFGQKETYDRQMWLQIRSYLVKESQFSKAKNWLAQ
                     QDFMERWMPEGNEHYGMNIHQFFAENTLNEWQEIYRHRENKSPSFKVIPSVDSHTWES
                     TYGNDGISFYAPIPMLYHGMKMKNAIKDGYWVDLQNNLVCFNPEINEKF"
BASE COUNT          140 a           86 c           58 g          171 t
ORIGIN      
        1 tgaaattttt catttatctc tggattaaag caaaccaaat tattttgtaa atcaacccaa
       61 taaccatctt taatcgcatt tttcattttc attccgtgat aaagcatagg aattggcgca
      121 taaaatgaaa tgccgtcatt gccataagta gattcccaag tgtgagaatc gacactaggt
      181 attaccttaa atgaaggcga tttattttcc ctatgtcgat aaatttcttg ccactcattt
      241 aatgtatttt cggcaaaaaa ttgatgaata ttcattccat agtgttcatt accttctggc
      301 atccatcttt ccataaaatc ttgttgtgcc aaccaatttt ttgcttttga aaattgagat
      361 tctttaacta aataacttct aatttgtaac cacatttgcc tatcataagt ttctttttgc
      421 ccaaattttg ctaattcacg aaaatcatcg tgtct
//
LOCUS       DANLXF010000426          448 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.427, whole genome shotgun sequence.
ACCESSION   DANLXF010000426 DANLXF010000000
VERSION     DANLXF010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.427"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..427)
                     /locus_tag="PXD66_002631"
     CDS             complement(<1..427)
                     /locus_tag="PXD66_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL6320865.1"
                     /translation="MGRPELERAVLSVQNQTYPCKHYVFVDGEKYHQQAADILKNFPS
                     VIVTYLPMNTGADGWVNSSINAIAPFLVKEDIICYLDDDNWFEPTHVESGVHALLEMQ
                     SDYAYSLRNFYDLDGEFLCIDTIESLGEYNCVYQEPLECN"
BASE COUNT          164 a           91 c           59 g          134 t
ORIGIN      
        1 aattacattc taatggttct tgataaacgc aattatattc tcccaacgat tcaatcgtat
       61 caatacacaa aaactcgcca tctaaatcat aaaaattacg taaagagtaa gcataatcag
      121 attgcatttc taataaagca tgcacaccag attcaacgtg agtaggttca aaccaattat
      181 catcatccag ataacaaata atatcttctt tcactaaaaa tggtgcaatt gcattaatgc
      241 tgctatttac ccaaccatca gccccggtat tcattgggag ataagtaaca ataacacttg
      301 gaaaattctt taaaatatca gccgcttgtt ggtgatattt ctctccatca acaaaaacat
      361 aatgtttaca aggataagtc tgattttgta cgcttaaaac agctctttct aattcaggac
      421 gaccaattgt tgaactgata acagctac
//
LOCUS       DANLXF010000427          443 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.428, whole genome shotgun sequence.
ACCESSION   DANLXF010000427 DANLXF010000000
VERSION     DANLXF010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.428"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            55..>443
                     /locus_tag="PXD66_002632"
     CDS             55..>443
                     /locus_tag="PXD66_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HDL6320866.1"
                     /translation="MSIKKVTLASLILLGLTACSSGGSGSSSNNVAKPNTKPQTTQQQ
                     TDKAAAEKAAKDKAAKEKAEAERLAAEKAAKEKAEAERLAAEKAAKEKAEAERLAAEK
                     AAKEKAEAERLAAEKAAKEKAEAERLAA"
BASE COUNT          172 a           76 c          118 g           77 t
ORIGIN      
        1 atgcgtgtgt tttatacaca tctatttttg tttatcttac aaaaggaaca ttttatgtct
       61 atcaaaaaag taactttagc cagtttgatt ttattgggtt taacggcgtg tagtagtggt
      121 ggttcgggca gtagctcaaa caatgtggca aaaccgaata ctaaaccgca aactactcag
      181 caacaaaccg ataaagcggc agcagaaaaa gcagcaaaag acaaggcagc gaaagagaaa
      241 gcagaagcag aacgtttagc agcagaaaaa gcagctaaag agaaagcgga agcagaacgt
      301 ttagcggcgg aaaaagcagc gaaagaaaaa gccgaagcgg aacgtttagc ggcggaaaaa
      361 gcagcgaaag aaaaagccga agcagaacgt ttagcggcgg aaaaagcggc gaaagagaaa
      421 gcggaagcag aacgtttagc ggc
//
LOCUS       DANLXF010000428          398 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.429, whole genome shotgun sequence.
ACCESSION   DANLXF010000428 DANLXF010000000
VERSION     DANLXF010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.429"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(3..>398)
                     /locus_tag="PXD66_002633"
     CDS             complement(3..>398)
                     /locus_tag="PXD66_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HDL6320867.1"
                     /translation="GKVDLALLLNMAQFDPQNLGNMQAVLQALSTSKFTSNINRQYAE
                     DIIRQVSIVTEKLGEEEAKAFAKQQVDAVFLNAGVEQYGLRKVDDNNVKIELTIDNGK
                     VNLNGRELTEDELQMALFMIVMGAGSLGQ"
BASE COUNT          105 a           88 c           65 g          140 t
ORIGIN      
        1 gattattgcc cgagtgagcc tgctcccatc acaatcataa acagagccat ttgaagttca
       61 tcttcggtca gttcacgtcc gtttaaattc acttttccgt tatctatagt taattcaatc
      121 ttcacattgt tgtcatctac ttttcttaat ccgtactgtt caactcccgc attgagaaaa
      181 acagcatcaa cttgttgctt ggcaaatgct ttcgcctctt cttcgcctaa tttttctgta
      241 actattgaga cttggcgaat aatatcttcc gcatattgac ggttgatatt tgaagtgaat
      301 ttacttgttg aaagggcttg caataccgct tgcatattac caagattttg tgggtcaaat
      361 tgagccatat tcagtaataa agccaaatca actttgcc
//
LOCUS       DANLXF010000429          369 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.430, whole genome shotgun sequence.
ACCESSION   DANLXF010000429 DANLXF010000000
VERSION     DANLXF010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.430"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..>369
                     /locus_tag="PXD66_002634"
     CDS             <1..>369
                     /locus_tag="PXD66_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL6320868.1"
                     /translation="AGQERCIADEVPFEIPQNWIWVRLENYSLNHDRRRKPVSVAQRS
                     QQNKLYDYYGATGAIDKVASYIFDGKFILIGEDGGNFFTKKDVAFIVEGKFWANNHVH
                     VLSVDFNLEKYFCYYLNALNL"
BASE COUNT          125 a           38 c           79 g          127 t
ORIGIN      
        1 gcaggtcagg aacggtgtat cgccgatgaa gtgccatttg agattcctca aaattggatt
       61 tgggtgaggt tggaaaatta ttctttaaat catgatagaa gaagaaaacc agtatcagtt
      121 gcacagcgta gccaacaaaa taaattgtat gactattatg gagcgactgg agcaatagat
      181 aaagtggcaa gctatatttt tgatggtaag tttattttaa ttggagaaga tggaggtaat
      241 ttcttcacta aaaaagacgt tgcttttatt gttgaaggta aattttgggc aaataatcat
      301 gttcatgtat tatcagttga ttttaatttg gaaaaatatt tttgttatta tttaaatgca
      361 ttaaattta
//
LOCUS       DANLXF010000430          357 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.431, whole genome shotgun sequence.
ACCESSION   DANLXF010000430 DANLXF010000000
VERSION     DANLXF010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.431"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..247
                     /locus_tag="PXD66_002635"
     CDS             <1..247
                     /locus_tag="PXD66_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HDL6320869.1"
                     /translation="LSILFVISPMALAYSCNDPIPYCKEMQSCEQAKFYLNQCNAGRL
                     DRDNDGIPCENVCGKKGKSEKKDDKKSDKSKSKKTSN"
BASE COUNT          118 a           59 c           76 g          104 t
ORIGIN      
        1 cctctcgatt ttatttgtta tttcgcctat ggctttggct tatagctgca atgaccctat
       61 cccttactgc aaagaaatgc aatcttgcga gcaagcaaag ttttatttaa atcaatgcaa
      121 tgccggtcga ttagatagag ataacgatgg cataccttgt gaaaatgttt gtggcaagaa
      181 aggaaagtcc gagaaaaaag acgataagaa atcagataaa tcaaaatcta aaaaaacttc
      241 aaattagaga caggcgttgt gcctgattaa aaggggtaat gatgaataac cagtcagttt
      301 ggcacttatt ttgcgatgaa tccggtataa gcggtaagcc tttttatgct ttcggtg
//
LOCUS       DANLXF010000431          355 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.432, whole genome shotgun sequence.
ACCESSION   DANLXF010000431 DANLXF010000000
VERSION     DANLXF010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.432"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            16..345
                     /locus_tag="PXD66_002636"
     CDS             16..345
                     /locus_tag="PXD66_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320870.1"
                     /translation="MKKALTLLALSLFALPVQADSLELARNKVKQIFISDEEPKVKDA
                     TWTAPFIFKVGVFDDGTKRDGYAEYVCQVLYDHGFKGKGVLVSVIDIKKLVQTGDWVD
                     LGKAKCQ"
BASE COUNT          120 a           58 c           81 g           96 t
ORIGIN      
        1 ataggggaaa ccgatatgaa aaaagcactt acactacttg cactatcact atttgcattg
       61 cctgtacaag ccgatagcct cgaacttgct cgtaataaag taaagcaaat ttttatcagt
      121 gatgaagagc caaaggtaaa agatgcgact tggactgcac catttatctt taaggttgga
      181 gtttttgatg acggcacgaa aagagatggc tacgccgaat atgtttgcca agttttatac
      241 gaccacggat ttaaaggtaa aggtgtgttg gttagcgtaa tcgatataaa aaaactagtc
      301 caaacaggtg attgggttga tcttggcaaa gctaaatgtc agtaatacat aaggg
//
LOCUS       DANLXF010000432          354 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.433, whole genome shotgun sequence.
ACCESSION   DANLXF010000432 DANLXF010000000
VERSION     DANLXF010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.433"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            31..107
                     /locus_tag="PXD66_002637"
     tRNA            31..107
                     /locus_tag="PXD66_002637"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:65..67,aa:Ile,seq:gat)
     gene            164..239
                     /locus_tag="PXD66_002638"
     tRNA            164..239
                     /locus_tag="PXD66_002638"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:197..199,aa:Ala,seq:tgc)
BASE COUNT          107 a           75 c           90 g           82 t
ORIGIN      
        1 aagaagacaa aaaaccgaag gtcatccgtt gggtctgtag ctcaggtggt tagagcgcac
       61 ccctgataag ggtgaggtcg gtggttcaag tccactcaga cccaccactc agaaggcaag
      121 cgaaagcgaa gcgactatga gtgaagaaaa cggaagatga aatggggata tagctcagct
      181 gggagagcgc ctgccttgca cgcaggaggt cagcggttcg atcccgctta tctccaccaa
      241 tcatcgtgac tttatcatca tacctaagat tacaagcggt taaaaatacc ggattatttg
      301 caattttagg tatgatgata ggcgaaagcc attatctttg ttctttaaca atat
//
LOCUS       DANLXF010000433          351 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.434, whole genome shotgun sequence.
ACCESSION   DANLXF010000433 DANLXF010000000
VERSION     DANLXF010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.434"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..>351
                     /locus_tag="PXD66_002639"
     CDS             <1..>351
                     /locus_tag="PXD66_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320871.1"
                     /translation="KISNHLITLAIYDENIQIQYSPQFIAKNLTMSDIQALQKLGFQF
                     SHSKEYLWKSYAGLGGKITQFKNRKEILEKYKFKQPIIAKAKSYQKSIEFSFDDTISV
                     IAAPITIPASIILFV"
BASE COUNT          133 a           61 c           52 g          105 t
ORIGIN      
        1 aagatcagta atcacctaat tactttagcc atttatgatg aaaatattca aattcagtac
       61 agtccgcaat ttattgcaaa aaatctcacc atgagcgata ttcaagctct acaaaagtta
      121 ggttttcaat ttagccattc aaaagaatat ctatggaaaa gctatgcagg tcttggtggt
      181 aaaatcacac agtttaaaaa tcgcaaggaa atactagaaa agtataaatt taagcaacct
      241 ataatcgcta aagcgaaaag ttatcagaaa agcatagaat ttagctttga tgacaccatt
      301 tcggtcattg cagcaccaat tacgattccc gcatctatta ttttatttgt a
//
LOCUS       DANLXF010000434          347 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.435, whole genome shotgun sequence.
ACCESSION   DANLXF010000434 DANLXF010000000
VERSION     DANLXF010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.435"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..>347
                     /locus_tag="PXD66_002640"
     CDS             <1..>347
                     /locus_tag="PXD66_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HDL6320872.1"
                     /translation="IQKNRVITLRADRIIKQFDDLALAENYAKNIPQDVFYLFDSLLN
                     QQRKEKITPIYKIGLCFTGFKQARKNELIQLAVDNDLRVVQNVTGAVDFLIFDKESKT
                     VGPAKLAKAEKLG"
BASE COUNT          134 a           48 c           60 g          105 t
ORIGIN      
        1 tattcagaaa aatagagtta tcacattaag agctgatcgc attattaaac aatttgatga
       61 tttagctctt gccgaaaatt atgccaaaaa tattcctcaa gatgtgtttt atctatttga
      121 ttctcttctg aaccagcaac gaaaagaaaa aataacaccg atctataaaa tcggtttgtg
      181 ttttactgga tttaagcaag ctagaaaaaa tgaattaatt caactagcag tagataatga
      241 tttgagagta gtacaaaacg taactggtgc agttgatttt ttaattttcg ataaagagtc
      301 gaaaactgtt ggaccagcaa aactagcaaa agctgaaaag ttaggaa
//
LOCUS       DANLXF010000435          337 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.436, whole genome shotgun sequence.
ACCESSION   DANLXF010000435 DANLXF010000000
VERSION     DANLXF010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.436"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..>337
                     /locus_tag="PXD66_002641"
     CDS             <1..>337
                     /locus_tag="PXD66_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL6320873.1"
                     /translation="PRITLTKLESYLVPVPPLLEQQRIVQKIEELLPLVERYEQTEQQ
                     LTKLNNTFPEQLKKSVLHAAIQGKLTEQDPNDELASCLIERIKAEKNRLIAEKKLKKP
                     KSVSEIVMR"
BASE COUNT          126 a           73 c           62 g           76 t
ORIGIN      
        1 ttccaagaat aacattaact aaattagaat cttatttagt cccagttccg ccactcctag
       61 aacaacagcg cattgtgcaa aaaatcgagg aattattacc gcttgttgaa cgctacgagc
      121 aaaccgaaca gcaactcact aaactcaaca atacattccc cgaacagctg aaaaaatcgg
      181 tgttacacgc agctattcag ggtaaattaa ccgagcaaga tccgaatgat gaactggcat
      241 cttgcctgat tgagcggata aaagcggaga aaaatcgtct tattgccgag aaaaaactca
      301 aaaaaccaaa aagtgtttca gagattgtaa tgcgtga
//
LOCUS       DANLXF010000436          332 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.437, whole genome shotgun sequence.
ACCESSION   DANLXF010000436 DANLXF010000000
VERSION     DANLXF010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.437"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            21..326
                     /locus_tag="PXD66_002642"
     CDS             21..326
                     /locus_tag="PXD66_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320874.1"
                     /translation="MSKLNKLLSRKTSRKIIPEQVNFDINLPIYIPLFLSDKTAKIQA
                     VWELEQENGERDAGAVKRNRIQFRYLPEGVHKLTLIVGTPLLSKGSKVYQCLIKVNK"
BASE COUNT          123 a           46 c           60 g          103 t
ORIGIN      
        1 attttacaaa ggaacattct atgtctaagt taaacaaatt attatcgaga aaaacatcgc
       61 gtaaaattat tcctgagcaa gttaattttg acattaactt gcctatctat atcccgctgt
      121 ttttatcaga taagacagcg aaaattcaag cagtatggga gttagaacaa gaaaatggtg
      181 agcgtgatgc tggcgctgta aagcgaaatc gtattcagtt tagatacttg ccagaagggg
      241 tacataaatt aacattaatt gttggtacgc ctttattatc aaaaggaagt aaggtttatc
      301 aatgcctaat taaagttaat aaataatatt aa
//
LOCUS       DANLXF010000437          328 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.438, whole genome shotgun sequence.
ACCESSION   DANLXF010000437 DANLXF010000000
VERSION     DANLXF010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.438"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..>328
                     /locus_tag="PXD66_002643"
     CDS             <1..>328
                     /locus_tag="PXD66_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320875.1"
                     /translation="GQAGSGKSHLLGDFVQKRQNSGKYSLLLLGQDFTEKSNAWNQIL
                     SKQLNLNITPEVFLSALDSIGEMQKERVILAIDALNEGEGKSLWNNQLNGLIELVSQY
                     PNIGLVL"
BASE COUNT          114 a           46 c           66 g          102 t
ORIGIN      
        1 ctggtcaagc tggtagtgga aaatctcatt tattgggtga ttttgtccaa aaaagacaaa
       61 attctggaaa gtattctttg ttgttattgg ggcaggattt tacagaaaaa tccaatgctt
      121 ggaatcaaat cttatccaaa caattaaatc ttaatatcac acccgaagta tttttatccg
      181 cattagatag tatcggagaa atgcaaaaag aacgggttat tttagccata gacgccttaa
      241 atgagggtga agggaaatca ttatggaata accaacttaa tggattaatt gagttggtaa
      301 gtcaatatcc aaatattgga ctggttct
//
LOCUS       DANLXF010000438          327 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.439, whole genome shotgun sequence.
ACCESSION   DANLXF010000438 DANLXF010000000
VERSION     DANLXF010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.439"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
BASE COUNT          119 a           48 c           58 g          102 t
ORIGIN      
        1 ttattctttt ttcagcaatt gtaataggtt tattcctttt tcaagaatca agattaagag
       61 atccaaacaa ccattcagtg atatatttag agcagattca agaaatgtgc agcttagata
      121 aaataggctc ataccagatt gattttgtac gacaaagtgg gaatgagtac aaagagagta
      181 ttcttgttaa tgattttgag ttagcaataa aaaccgtatt atccacgtta agaagggcaa
      241 aaatagatag tgtgtcagta atatctaaca cacctaattt atttattatc caccgagctt
      301 tctacaacgc aagaggtagt caagaag
//
LOCUS       DANLXF010000439          324 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.440, whole genome shotgun sequence.
ACCESSION   DANLXF010000439 DANLXF010000000
VERSION     DANLXF010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.440"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..>324
                     /locus_tag="PXD66_002644"
     CDS             <1..>324
                     /locus_tag="PXD66_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL6320876.1"
                     /translation="DSLYPHIERLIQELKIHKQTDDCQLFNLNLFSRYTRRKGQPMSQ
                     NQLTHIFRHISDLVGFTSSPHRFRHTVATQLMKKPENVYVVKKLLGHKDISVTLGYIE
                     YDVEML"
BASE COUNT          107 a           57 c           59 g          101 t
ORIGIN      
        1 cgattcactt tatccccata tagaaaggct gattcaggag ttaaagatcc ataaacaaac
       61 ggatgattgc cagttattta acctcaattt attttctcgt tatactcgta ggaaaggtca
      121 gccgatgagc caaaaccagc ttactcatat atttaggcat atttccgatt tggttggctt
      181 cacgtcatca ccacatagat ttcggcatac agtagctact caattgatga agaaacctga
      241 aaatgtttat gtagttaaga aattattagg tcataaagat atcagcgtta cattaggata
      301 tattgaatac gatgtggaaa tgct
//
LOCUS       DANLXF010000440          317 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.441, whole genome shotgun sequence.
ACCESSION   DANLXF010000440 DANLXF010000000
VERSION     DANLXF010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.441"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
BASE COUNT          111 a           46 c           57 g          103 t
ORIGIN      
        1 attattagcc tatgttaaac ctgagtactg gaaatgctca gacttattta gttatcaaaa
       61 ttgggtaaga agcagtgata aacagccctt tattttgtat gctaaatacc aagataagat
      121 gttatgtgaa tcaaagggtc aatggagatg gttaaaaaaa cagtcgagaa tagttgtaga
      181 tcaatgtttt ctacatgtta atcaatggca gttactaaca gaaattcaaa ttcccttcaa
      241 gatcccagta tgtgtcatat gtttcattct tactaatttt agtatgatca gcagtgaatt
      301 agaacgttct agacaac
//
LOCUS       DANLXF010000441          315 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.442, whole genome shotgun sequence.
ACCESSION   DANLXF010000441 DANLXF010000000
VERSION     DANLXF010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.442"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..>315
                     /locus_tag="PXD66_002645"
     CDS             <1..>315
                     /locus_tag="PXD66_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011150324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HDL6320877.1"
                     /translation="IFISAGGVRSIWLRHNLANFKQRLNALEKAVAEKGIILSENQVQ
                     ALERKKEDDVACGEIETAHPGYLGSQDTFYVGNLKGVGRIYQQTFIDTYSKVAFAKLY
                     TMK"
BASE COUNT           95 a           53 c           76 g           91 t
ORIGIN      
        1 atttttattt cagcaggtgg tgtgcgttct atttggctac gccataatct cgctaacttt
       61 aaacagcgtt taaatgcgtt agaaaaagca gtggctgaga aaggcattat tctgagtgaa
      121 aaccaagttc aagccttgga gcgtaaaaaa gaagacgatg tggcttgtgg cgaaattgaa
      181 acggctcatc cgggctatct aggttcacaa gataccttct atgtggggaa tcttaagggg
      241 gttggacgca tttatcaaca gacctttatt gatacttata gtaaagtcgc atttgcaaaa
      301 ctgtatacga tgaag
//
LOCUS       DANLXF010000442          314 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.443, whole genome shotgun sequence.
ACCESSION   DANLXF010000442 DANLXF010000000
VERSION     DANLXF010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.443"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
BASE COUNT          101 a           47 c           56 g          110 t
ORIGIN      
        1 ctgatattaa tttgcctgat ctggaagttc ctacggcgga atcaattata tcccctttta
       61 aaaaattgct accagattta cagaattttg aactgaataa acgaaccgca caatgccctg
      121 tttggacatt taatattgaa tacttgagat ttcattatga acttagagaa cattgcgatt
      181 tgatagaacc acataaagaa atcttaaagc tattattcgg cattatttgg gcgtttattg
      241 ctttacgtta tgtattaagt gcttaggagg ataaatgttt aaagtgattt ttatggcact
      301 atcagcaatt gtga
//
LOCUS       DANLXF010000443          312 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.444, whole genome shotgun sequence.
ACCESSION   DANLXF010000443 DANLXF010000000
VERSION     DANLXF010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.444"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            complement(<1..>312)
                     /locus_tag="PXD66_002646"
     CDS             complement(<1..>312)
                     /locus_tag="PXD66_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL6320878.1"
                     /translation="IARMDADDISLPTRLSEQILYLEENHLDLCGCNALLFSEHGNLG
                     LTNKLNSHEALEKLMVAGDIGIIHPTFLARRKVFVESGGYNPLALHAEDMEFIAHILS
                     MG"
BASE COUNT          110 a           66 c           42 g           94 t
ORIGIN      
        1 cccattgata aaatatgggc aataaattcc atatcttccg catgtaaagc taagggatta
       61 taccctccag attcaacaaa tacttttctc cttgctaaaa aagtagggtg aattattcca
      121 atatcaccag ccaccataag cttttctaaa gcctcatgac tattcaactt atttgtaaga
      181 ccaaggttgc catgttcaga aaaaagcaaa gcattacaac cgcataagtc taaatggttt
      241 tcttctaaat ataaaatttg ctctgacaat ctagtaggta aagatatatc atcagcatcc
      301 attctcgcta ta
//
LOCUS       DANLXF010000444          302 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.445, whole genome shotgun sequence.
ACCESSION   DANLXF010000444 DANLXF010000000
VERSION     DANLXF010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.445"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            4..>302
                     /locus_tag="PXD66_002647"
     CDS             4..>302
                     /locus_tag="PXD66_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HDL6320879.1"
                     /translation="MYSKNTTKSEIDKLDQKILRWLIKDARTPYAEMAKVFGVSAATI
                     HVRVEKMRQAGIIESTKIVVDERKVGYDVCCFIGIILKSAKDYDKVIAKLSEFNEV"
BASE COUNT          103 a           45 c           67 g           87 t
ORIGIN      
        1 aaaatgtaca gtaaaaacac gactaaatcg gaaattgata agctggatca aaaaatttta
       61 cgttggctaa ttaaagatgc tcgcacgcct tatgcggaaa tggcaaaagt ttttggtgtg
      121 agtgccgcaa ctatccatgt ccgtgttgaa aaaatgcgcc aagccggcat tattgaatct
      181 accaaaatcg tagtggacga gcgtaaggtt gggtatgacg tgtgttgctt tattggcatt
      241 attttaaaat cagccaaaga ttatgataaa gtaattgcta aacttagtga atttaatgaa
      301 gt
//
LOCUS       DANLXF010000445          300 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.denovo.446, whole genome shotgun sequence.
ACCESSION   DANLXF010000445 DANLXF010000000
VERSION     DANLXF010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.denovo.446"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..>300
                     /locus_tag="PXD66_002648"
     CDS             <1..>300
                     /locus_tag="PXD66_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6320880.1"
                     /translation="IASDWEIIEAAQNKVKAELIRKSEKKAELCLNVAQAVLITNQKV
                     QKDTFSMCGEFSTEIVFSDSKAYSLAVCGKVKGKDIIGNPIESDYIYQRATKDFY"
BASE COUNT          113 a           39 c           63 g           85 t
ORIGIN      
        1 tattgcatcg gattgggaaa taatagaagc ggctcaaaat aaggtgaaag cagagcttat
       61 aagaaaatca gaaaaaaaag cagagttatg tttaaatgtt gctcaggctg tcttaattac
      121 taatcaaaaa gtgcaaaaag atactttctc aatgtgtggt gaattttcga cagagattgt
      181 cttttctgac tctaaagcat atagccttgc agtatgtgga aaggttaaag gtaaagatat
      241 tatcggaaat ccgatagaga gcgactatat ataccaaaga gcaactaagg atttttactt
//
LOCUS       DANLXF010000446         2871 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749863-rid16718683.guided.254, whole genome shotgun sequence.
ACCESSION   DANLXF010000446 DANLXF010000000
VERSION     DANLXF010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749863
            Sequence Read Archive: SRR17934107
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574591.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574591.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 20:57:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 35 of 60
            Pseudo Genes (incomplete)         :: 17 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 1 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749863-rid16718683.guided.254"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: CO"
                     /collection_date="2020-01-27"
                     /collected_by="Colorado State University Veterinary
                     Diagnostic Laboratory, Fort Collins, CO"
     gene            <1..650
                     /locus_tag="PXD66_002649"
     CDS             <1..650
                     /locus_tag="PXD66_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HDL6320881.1"
                     /translation="KAAKEKAEAERLAAEKEVDPIAIIKAKGIDDTNYGLKLGNSKET
                     IEGKVTVRTQSYLYNQPYSVVTAQAVQRNGWENGNYIDKVDGTVTVKGLKTEKLPTEG
                     TATYSGKAFNHDGAKGFNGGDLTYNVNFSKRTGSGRVEGEFGNLIVLEEGTINNTQIS
                     STVHGNNPLNLSGKYTVEFFGKNAEEIAGEIQTKANLTGEVSNFGLAGTRGDIQK"
     gene            846..2255
                     /locus_tag="PXD66_002650"
     CDS             846..2255
                     /locus_tag="PXD66_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL6320882.1"
                     /translation="MKKYPLLFSLFSPVVWAEKVIEHNTDPISFERQPQAVKFEQNVV
                     KNSQKPTAYQPAYSVNQNVEQQINQAILSQDWATLEPLLSRYPQTENFDPLLWHYAQA
                     ALAYAKKDHQTAVFHYRQLLAKPPSLVYPRFDLALILAEDQQFRAAEQEFKAIQAHLP
                     PELAKIARNTQVQIAENEAWQPDFYLQYTQTDNVNNASSSPIVNVNGRIFRKNADSLP
                     QSAKGVRYGLGLSKLHNVKGNHFVGLTLNYSGIYYWDNRDYREQSVSVSPNYSYRTVA
                     YRLTFSPFIEQNWLGSSRYNVQFGATLNGLRYLNSQWLVSGSFTHLQKRYFEELTASR
                     YNSYQHQLSGGVQWQAVKNGRFFANLDGVREIAREKAQSSNKWLARVGAVFQGERWAT
                     QASIGFGKRYFADKHYLFGYKRLDTEYQANLAVWNRTWHWQGIVPKLNFRYQKIDSNI
                     ADFYSRQNKEVFFTLEKLF"
     gene            2401..>2871
                     /gene="gpt"
                     /locus_tag="PXD66_002651"
     CDS             2401..>2871
                     /gene="gpt"
                     /locus_tag="PXD66_002651"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDL6320883.1"
                     /translation="MSEKYTNEKYVVTWDMFHMHARKLAENLLPATQWKGIIAVSRGG
                     LFPAAVLARELGIRHVETVCIASYDHDSQGELNVLHAADTDGEGFIVVDDLVDTGNTA
                     KEIRRMYPKARFVTVFAKPHGAPLVDDYVIDIPQETWIEQPWDLGITFVQPLARR"
BASE COUNT          867 a          550 c          668 g          786 t
ORIGIN      
        1 aaaaagcggc gaaagagaaa gcggaagcgg agcgtttagc agcagaaaaa gaagtagatc
       61 ctatagctat tattaaagca aaaggtattg atgatacaaa ttacggctta aaacttggta
      121 atagtaaaga aactattgag ggtaaagtca cagtcagaac acagagttat ttatataatc
      181 agccatattc tgtagtaaca gcacaagcag ttcaaagaaa tggctgggaa aacggtaatt
      241 atattgataa agttgatgga acagttacag ttaaaggatt gaaaacagag aaattaccaa
      301 cagagggtac tgcaacatat tcaggaaaag catttaacca cgatggagct aaaggtttta
      361 atggtggtga tctaacctat aatgttaatt tctctaagcg taccggttca ggtagagttg
      421 agggagaatt tggaaactta atcgtattag aagaggggac gattaataat actcagattt
      481 cttcaacagt acatggcaat aatcctttaa acctttctgg aaaatataca gtcgaatttt
      541 tcggtaaaaa tgcagaggaa attgccggtg aaattcagac aaaagcaaat cttactgggg
      601 aagtgagtaa ttttggactt gccggcactc gtggcgatat ccaaaaataa ccttttctaa
      661 cgttcaatgc cgtttaacga aaaattaaac ggcatttgtc ttgatattgc ttgtagatac
      721 cataagacac aatagtttag atttcaattt acagcttcta ataaccctct cccacaaggg
      781 gagagagtga taaatcccgt agaggcgaac atataggtcc gcccctacca attttcttta
      841 tctttatgaa aaaatatccc cttttatttt cgctgttcag ccccgttgta tgggcagaaa
      901 aggtgattga acacaacact gacccgattt cctttgagcg tcagccgcaa gcggttaaat
      961 ttgagcaaaa tgttgtaaaa aattcacaaa aaccgaccgc ttatcagcct gcgtattcag
     1021 tcaatcagaa tgttgaacag caaattaacc aagcgattct cagccaagat tgggcaacgc
     1081 ttgaaccgct tttaagccgt tatccgcaaa ctgaaaattt tgacccgttg ttgtggcact
     1141 atgcgcaagc cgcacttgcc tatgcgaaaa aagatcatca aaccgcagtt tttcactatc
     1201 gccaactgtt agctaagccc ccgagtttgg tttaccctcg ttttgatttg gcgttgattt
     1261 tggcagaaga tcagcaattt cgtgcggcag agcaggaatt taaggctatt caagcccatt
     1321 taccgccgga acttgctaaa attgcccgca atactcaagt ccaaattgct gaaaatgagg
     1381 cgtggcagcc cgatttttat ctgcaatata cccaaacaga taacgtgaat aatgcctcat
     1441 cttcgccgat tgtgaatgta aatgggcgga ttttccgcaa aaatgcagac agtttgccac
     1501 aatcagcaaa aggcgtgcgt tatggcttag ggttgagtaa attgcacaac gtgaaaggca
     1561 atcattttgt cggtttaacg ctcaattata gcgggattta ctattgggat aaccgagatt
     1621 accgtgagca atccgtatcc gtttcgccga attatagcta ccgcaccgtc gcttatcggc
     1681 tgactttttc gccttttatt gagcaaaatt ggctggggag tagccgttat aatgttcaat
     1741 ttggggcaac actcaacggt ttgcgttacc ttaattcgca atggttggtg tcggggagtt
     1801 ttacccattt gcaaaaacgg tattttgagg agctaaccgc aagccgttat aacagctatc
     1861 agcaccaact cagtggcggt gtgcagtggc aagcggtcaa aaatgggcgg ttttttgcaa
     1921 atttagacgg cgtgcgtgaa attgcacggg aaaaagcaca aagctcgaat aagtggctgg
     1981 ctcgagtggg ggcggttttt caaggcgaac gctgggcaac gcaggcaagt atcggctttg
     2041 gcaagcgata ttttgccgac aagcactatt tgtttggcta taagcggtta gacacggaat
     2101 atcaagccaa tttagcggtt tggaaccgca cttggcattg gcagggcatt gtgccgaagc
     2161 tgaatttccg ttatcaaaaa attgacagca atattgctga tttttactct cgccaaaaca
     2221 aggaagtctt tttcacttta gaaaagctat tttagtgttg gttttttgtg ctaagcatac
     2281 gattgcgtag ctgccggcac aaaaaaataa atgaaatgat ggcgaaaaaa gcattttcgg
     2341 gttatcctag tgcaattttt tatatcgggg tagcccccaa ttttatatta ggttgtttct
     2401 atgagtgaaa aatacacaaa tgaaaaatat gtcgtgacgt gggatatgtt ccatatgcac
     2461 gctcgcaagt tggcagaaaa tttattacct gccacacagt ggaaagggat tattgcggtg
     2521 agccgtggcg gcttgtttcc ggcagccgtt ttagcacgtg agttgggtat tcgccacgta
     2581 gaaacagtgt gcattgccag ctacgatcac gatagccaag gtgagttaaa tgtgttacac
     2641 gcagccgata ccgatggcga aggctttatt gtggtagatg atttagtgga taccggcaac
     2701 acggcaaaag aaattcgcag aatgtacccg aaagctcgtt ttgtaaccgt gtttgcgaaa
     2761 ccgcacggtg caccgttggt tgatgattat gtgattgata ttccacaaga aacgtggatt
     2821 gagcagcctt gggatttagg cattaccttc gttcagcctt tggcacgtag a
//
